LOCUS       DAZUMK010000001       129783 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000001 DAZUMK010000000
VERSION     DAZUMK010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 129783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 129783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..129783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.001"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            197..1084
                     /gene="hslO"
                     /locus_tag="ACG808_000001"
     CDS             197..1084
                     /gene="hslO"
                     /locus_tag="ACG808_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFS5045668.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            1085..2089
                     /gene="dusB"
                     /locus_tag="ACG808_000002"
     CDS             1085..2089
                     /gene="dusB"
                     /locus_tag="ACG808_000002"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFS5045669.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            2200..3708
                     /gene="lysS"
                     /locus_tag="ACG808_000003"
     CDS             2200..3708
                     /gene="lysS"
                     /locus_tag="ACG808_000003"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFS5045670.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMKPIDK"
     gene            3943..4215
                     /locus_tag="ACG808_000004"
     CDS             3943..4215
                     /locus_tag="ACG808_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6462 family protein"
                     /protein_id="HFS5045671.1"
                     /translation="MISDRTKAYYDLKKRNDVRESAKRLRRQFLRYKDAEIVYSITHK
                     KLLELAGKAGAIYRMDGTVLINRDIFDEYLEQFHEPSTLASQEDKE"
     gene            4266..4409
                     /locus_tag="ACG808_000005"
                     /pseudo
     CDS             4266..4409
                     /locus_tag="ACG808_000005"
                     /inference="COORDINATES: protein motif:HMM:NF039789.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6462 family protein"
     gene            4485..4697
                     /locus_tag="ACG808_000006"
     CDS             4485..4697
                     /locus_tag="ACG808_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFS5045672.1"
                     /translation="MRQGALTLDAQTGRMDIRFDLENYYGGLHCGECLDILINGDWVP
                     TRIEMGDGWYLTGIKTDNLEGLIVRI"
     gene            4779..5852
                     /locus_tag="ACG808_000007"
     CDS             4779..5852
                     /locus_tag="ACG808_000007"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045673.1"
                     /translation="MKCLMKYQWVKLPRNRLPEGKGVMGAWARLASRAAFRKGQALYC
                     GHINAVSPGMWSGGVVGLKSVLGLKSRQKALEVLQTLADLGYVQYTLDSKTKKLTYQI
                     TDWVVKCSGEECMQGAVYATEGYGFLCLPRNITERLAEKRYIFEESDAWMDLWCHSVW
                     EDPNNAFSFMAPTVQYGKYGALLTLETLGQRWGWEKTKVWRFMKKHGDVFALYRLPGS
                     YGCLIFNKLYPAGSEVSVPSQEEVMCILGKIRICGANTQKSGSDHEHMNKLITWYSRR
                     LTGSNLSDAAIQDAENRVAFSDPIIRAYLSPCRNCKNCGYDCQGKGYMTQAVIETSKI
                     RGPCEPVDITKIAKECFTYEQAG"
     gene            5836..6468
                     /locus_tag="ACG808_000008"
     CDS             5836..6468
                     /locus_tag="ACG808_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045674.1"
                     /translation="MSKQDEKKLYEQQENRILAQSDAFISLLTKRGILGDQKIDDEKV
                     RAAQKEKKRNAYHNTQMLLKNYRKMVWVFSCYPEEILQELETPFENFDRMVDRLDLEM
                     AIGNKRMESRLEAAARSRLLLDRFNEAMAVLRKEPTRGEKLYQLIYTAYLAPEKLKLE
                     EILFRLDLSQRHYYRLREQAISILSIRLWSAPSEDVDVWLELLTLFEHLE"
     gene            6656..7015
                     /locus_tag="ACG808_000009"
     CDS             6656..7015
                     /locus_tag="ACG808_000009"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045675.1"
                     /translation="MVKQIKRGGGRMKTAYWSLVGMASAMLISLQPVMADTIWNRFST
                     IMKDVYGQLVGISTIVAVTAAAIALIVRMISRNQRAVDEATSWLKRIVVTWIVLNSLG
                     FVVAYLQPLIAGGNYTG"
     gene            7053..9143
                     /locus_tag="ACG808_000010"
     CDS             7053..9143
                     /locus_tag="ACG808_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045676.1"
                     /translation="MLDWIFEGIVTWISSIVSQMMDAVSGLFLQALGTDMTAMEEYFP
                     FVSKAFTVMQYTAWAILFLVTVWQLFRVFGGPITEAENPWVLLARGSIFALLIGYAKP
                     IFMIVLDIARAPYTALMEITMSAEDFTFAGVEQALSNGLTTIVAIVSVVGLLLLIILE
                     IALGWNYFKLLLETVERYIVVGVLCYTSPLAFSMGASKTTAPVFKSWCRMVGSQLLLL
                     VMNVWFLRGFNSSMGQYIGNGGALSTGQGSIFLWLFCALAFLKTAQKFDSYLAAMGLN
                     VAQTGSSMGMELLMAARVISGVGGGVKNAGSMFHSASTATGTGAAASGFASGFASRFK
                     PNSFVRDAVVDGGSRMGVGGSIGFVGRAFGGMAARNGATLTGDSISSVASRNPGVSGT
                     IAGDIADRSLAGYMPQLQGHQLKDTQITGGQISTTSIGADGKSTALEMYNASQFEKPD
                     APHSVVTASDGSQWYQMASGAGAGAFYDAPAFMGSPSEAAPVAAAFPDASEGTTLRTV
                     GDGVIEASSESGNTMWYNSAYYDEPDAPHTIMQSANGVDWYAMQQHAETPAFEQGEAA
                     FAYNQAQFQQFMPGYEQQVSSVDGSGRKDGHFEVRHENGSGTMFYDTSQYAAPRGDYQ
                     VFEDARGGQWYAIRGEAAVERKPVYQDGKPVYDGDSVRTVSVETVRYKNTPSRFGEPK
                     PRNHGEMKPPRRKR"
     gene            9252..11273
                     /locus_tag="ACG808_000011"
     CDS             9252..11273
                     /locus_tag="ACG808_000011"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFS5045677.1"
                     /translation="MAEPEKQQIGDGSDNYGQAAGQMAKAAKQAGQEAAKQTAAKGAE
                     ATANAAAATVKASVEGGKAAAEIAAGTAAGGPWGAILSAAWAMRHTLFKILVCICLFL
                     LFLITMIVSLPTIVSNSIFGLDGTKPTEGATLMSAYTEMSGAVSGVVDEGYNQSLSHV
                     DQLITNGGYDYDLSMDALINYAQSSAGYDVCYILAAYSASMQQQNTGKDDMVSKLRGC
                     AGEMFPVTSEEKEKEIIIPVSYYTYKPITLTVVTNKVQTGTINGTPQYRYDTASKTYY
                     LPDEAHSSDTAVTVDAYKEVAVDIPVYSGGKITGTTTANYYVANGQETLTPGTEIIKY
                     LECTIHPFDNTVVAKAFGIDLNAKYDQFNLTYGEVIQNMANALKRTLYGSVSGGQAVP
                     LTDAELIAFVNRQNCSATRKHILSTALSLVGKVPYFWGGKSAPGWNDEWNTPRLVTAA
                     GSSTTGTIRPYGLDCSGFSTWVFNTAVGVEIGAGCNGQYPNTYGISASELLPGDLGFL
                     ADDDGWGHVLIFAGYGESGERMWVHSSGGQGVILNTPSYEGSLSYRRLSIVDYDAPVL
                     GEISGTPISTLEVDVTHYCACAKCCGSNADGITASGKQAARGMVAMSSYYPFGTQIMI
                     NGTMYTVEDRGGSGIENDIHRVDIFVPDHNEALRLGRYKTTATIYRIGR"
     gene            11280..11702
                     /locus_tag="ACG808_000012"
     CDS             11280..11702
                     /locus_tag="ACG808_000012"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045678.1"
                     /translation="MDEKEYSNIYAIPANYTDSGKIFGGMLEPRNAVETGILLVILGY
                     PELMLIPMSGTIRIVVMTLTLLPLAVLSMMGIDGDSLFQYIGHIIRYFASRRKLHYRR
                     VGYRYDPKQLRKKKKGSSAKKKGKKTGKAGVRSGLHSG"
     gene            11599..14145
                     /locus_tag="ACG808_000013"
     CDS             11599..14145
                     /locus_tag="ACG808_000013"
                     /inference="COORDINATES: protein motif:HMM:NF024254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5045679.1"
                     /translation="MTQSSSEKRKKAARQRKKAKKPEKLEFVQDFIPVKSLQHGIIET
                     TDGRYIRILEIEPINFMLRSGEEQFNIISSFASWLKISPMRLQFKSITRKADSDKHVA
                     MIHEELEVEESEECRHLGEGYIRLIKDVGSREALTRRFFLIFQYEEIRRGDGDDFSQI
                     YGMMQTAEQNARAYFLQCGNTILQPKDPDEATAEILYMFFNRRSCVDEPFSSRVNRVV
                     VDTMVAKDKVIGVDPIPPIRMAHFLSPRGIDLTHHNYVVMDGLYYSFLFIKGNGYPGM
                     VRAGWMSALINAGDGIDIDVQLRRENRSKTIDKVAQRIRLNRTKLKSMQDTSTDYEEL
                     TNSIQAGYFIKNGIANNNEDLFYMSVFVTVSAKGYEELMWRKQQIVDMLKSMDMYVSD
                     CRFQQEAALRSVMPFLKIEPSLEKKSKRNVLTSGAASAYMFTSFEMSDDTGVLLGINR
                     HNNSLCIVDLFNTKKNKNANLNLLGTSGAGKTFTMQLLALRMRMRGIQCFILAPIKGH
                     EFRRACKKIGGEFIKIAPGSPHCINIMEIRHTISPEMELIDEIDYSEMDSMLARKIQQ
                     LMTFFSLLIPDMSNEEEQMLDEALIQTYAKFGITHDNDSLYVSRSVSPPKMKKMPIIG
                     DLHEELLKNPMTKRIAIIISRFVTGSAQSFNQQTNVDLSNKYIVLDLSELKGKLLPVG
                     MFIALDYVWDTVKADRTKKKAIMIDEIWQLIGASSNRMAAEFCLTIFKTIRGFGGAAV
                     SATQDLSDFFGLEDGKYGRAIINNSKNKIILNLEPDEAKYVQEVLKLTKTEIRSVTRF
                     ERGEALVCSNNNKVPVVIKASKEEQEMITTDRAELEALLKERQQEQTHSA"
     gene            14191..14742
                     /locus_tag="ACG808_000014"
     CDS             14191..14742
                     /locus_tag="ACG808_000014"
                     /inference="COORDINATES: protein motif:HMM:NF039505.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5697 family protein"
                     /protein_id="HFS5045680.1"
                     /translation="MLLQDEQYVVKWLSQYGALPKTQITRMLQKPAQTADKILRNLKK
                     QMRIADVSSGYYIGLDAMDKPDQRTILAVWVLLKFIDYVDPMAHYPAVYPSQLFFLKE
                     NIGYEIVVLYEGEQNLLKLLQPQEDLKYIIVLPRISMAYGMRLPHAPCLFATVDFQGD
                     EEPGVTFYSEEAVSDDKISGHDG"
     gene            14714..15385
                     /locus_tag="ACG808_000015"
     CDS             14714..15385
                     /locus_tag="ACG808_000015"
                     /inference="COORDINATES: protein motif:HMM:NF039349.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6100 family protein"
                     /protein_id="HFS5045681.1"
                     /translation="MIKFLDTMGKIVRLEKRLQEQLASIQWFCENGSTYEVYLHSLNM
                     EETAERLVLLTRALPAYTGVPNAKLEVERRIKDCIPVEIGFTAEGWFCLRIPALLPKK
                     SGGSADYIRSFLYPAMRAFFEKQLPVRYRDGILIYRHVYDKARPERKKRDHDNIEINM
                     VSDIVAMYVMTDDGPSVCSHYYCSAEGSEDRTEVYVVPKWDFPIWLVKETGIPDEGVL
                     LYEKR"
     gene            15372..15548
                     /locus_tag="ACG808_000016"
     CDS             15372..15548
                     /locus_tag="ACG808_000016"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045682.1"
                     /translation="MKKGKFNRKNICKNDAYRAEKKYISSERAKKPSIAAVWGRHYAG
                     NSDKESSHVVGKCL"
     gene            15565..16719
                     /locus_tag="ACG808_000017"
     CDS             15565..16719
                     /locus_tag="ACG808_000017"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045683.1"
                     /translation="MVHFRPGSQVWQIITLLSFVGEFPFKSLSLLGRERVYKALISRL
                     TTLQTIRNFNSGDEITCRLLTVSGKGAGKTIRLYKGALPILEWLYPGVYGYYMDSFWG
                     HRFPGDVSHRDRNHRVAEAAAMFLKAGMEARPYLLPRLQNREILQVVHGTPCFYLAKD
                     LKKVGEAEMNKTMFTRMAGALFSSGRCYAVYNTRDAVMKWSGMGEYKALHSLIELARL
                     NAGILEVDSAILFGQSGETALRTLLESDKTRRLEFRFDSIYRHVHFIPMNGDGIRQLR
                     LLSAPDWKAQLLELLFEPEVRSYDRGLFEYDACVNGVNILSHLDGDIARLIRFRDAIE
                     NQTGRFEVLCFPHQTHFLREYLGGLASIKTIGMDSVEAELCPERRNLFER"
     gene            16709..18571
                     /locus_tag="ACG808_000018"
     CDS             16709..18571
                     /locus_tag="ACG808_000018"
                     /inference="COORDINATES: protein motif:HMM:NF014581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFS5045684.1"
                     /translation="MKDKKRTVGITMAVILGCTAFFYLGGMLGQLFSNYAAWMQADGL
                     AGEAAMKPVSWNPVVCFPMAFTSDGLKGLLGILVLGGGVFAYVKLHDKFDGKSYDPRG
                     FTKSKTGIYGTASWMEEKEMHEVLEVSSVDKAEGTILGEYKGKAVCMPKDTRLNRHIA
                     IFGASGTMKSRAVIRNSLFQALKRGESVIITDSKAELYADTAEMYRQAGYEVKIFNLV
                     TPEHGDSWNCMSDLNGDTLMAQVLTNVIIGNTSSGKGDHFWDNGEGNLLKSLILLVDQ
                     DRTRSPDMKHLTAVYQLLTQNSERQLTAMFDKLPLDHPARAPFNLFAQSSDTVKSGII
                     LGLGTRLQVLQNQAVQRITSQSDIDLTSPARHKCAYYIILSDQDATMAFLSSLFFSFM
                     FIKLTRYADSRPNGRCDVPVNLILDEFNNVGRIGGAEDGSDFARSLSVVRSRDIRVML
                     AVQSLGQLQNRYPNNLWAEIIGNCDIQLMLGCTDEVTAEYISARSGDMSVQVNSTMTV
                     RQTIAIAQVIPQYRHTEGQGRRRLLTPDEVLRLPNEEMLCVIRGCNLLKLTKLDFTKH
                     PMSKQIVRTSIMDYRPATIFTAPILSEPVPVAEPEKKPARKKSLYSSAKPPAEF"
     gene            18592..20004
                     /locus_tag="ACG808_000019"
     CDS             18592..20004
                     /locus_tag="ACG808_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HFS5045685.1"
                     /translation="MATKKQELLPEEEVLQEAAPQTDVNPELEEMSQPEDSSPDTALP
                     QTDSETSTEGASSEEFRDAEAAEAPVSQIDMKTNDGQPPSESAEEISSQTDMESAEDD
                     TSSENASDKTTPQEGSPQTEPEKVKKPRGRRKTKENPSQSDTEADSGEENASDGVERA
                     PKPKRKRASARPVSPVLAIDDQLSVETDAEKAKNDLLDLLESQKTGRILTGTIQGVER
                     PEDNPSRSFAVIYHGEFKVIIPAEEAVEPPADYRGRLPEDVLHYMLTKRLGAEVDYIV
                     KGIDPASGIAAASRLDAMKAKRKQYYLGTDRDGNNLLYSGVCAEARIVSVIRAGIFVD
                     LFGLEIYIPLRELSYQRWMDAAAHFQPGQRILVKVLEVDRSDRNHVKATASVKQSGEN
                     PYEKALRRYSVGNRYVGTVSMVDTNGVFVALDGGIDCLCSYPKRGRPPRGSRVTVRIL
                     GINHDSNRIWGAITHIAAPR"
     gene            20041..20454
                     /locus_tag="ACG808_000020"
     CDS             20041..20454
                     /locus_tag="ACG808_000020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045686.1"
                     /translation="MNHCDLSPAEQKKRKEIAEHTRKMILSELKIESQDDISLMAGYR
                     DYYLQISFSELHPLLVFCLAKALEKPSTDRQRQMTNELNLHSILGSHAINDEVGCYSY
                     RATQWLDAELDAPRFFEILDRCVDEADRGFYKLAG"
     gene            20522..21268
                     /locus_tag="ACG808_000021"
     CDS             20522..21268
                     /locus_tag="ACG808_000021"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045687.1"
                     /translation="MKKCVLFVVLAVLLALTACSKDVGTSDDHGQESKHTAQSQTEKE
                     SEDEDLKETAPIEEPKPVSQSEAKEEVSLSDPVHETDQNSESGKEAQNRAEENAPEPK
                     QPAAGNGQPTPEPKQPEPPKPTETPAEPNQEETPVSATEPESPKTIYDYAFDAEAIRS
                     ELIALGQSMGLTHITDDDGTPCTPDTCSWASPVTASESFQGNSLKRALQNYVTSMPST
                     IASYGGTQITCFTIYVRDNGGGSYTFYFLY"
     gene            21341..25873
                     /locus_tag="ACG808_000022"
     CDS             21341..25873
                     /locus_tag="ACG808_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006506531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS5045688.1"
                     /translation="MKKIIKEKGKRLLAGFLSLLTVIGLIPTTVFAFTPSEGQTVSSF
                     YGDYYVSADGNYYYSASSYSFLVYDSDGNTSVQTISAGNARKKYMISNGSESRFVYCI
                     ESGINYGTSDNGYRSQSGKNSAYFQNLPYSAQYGIMLTSVYGWQPGKSSPVSGANEDD
                     YAVATQVLLWEYQQQLRTSPQDLHANSAGIRADNYLRTIQGRPAEQCYNWILGQMSQH
                     TTIPSFASGKSSSAQVHTLKYDPATKKYSLTLTDTNNTMADLKFSSSDGITVSRSGNK
                     YTFTSSKMITSPVSISVQKNVPGVKNDMLIWGRVGFQTMMCGADDPVVFYMKIDTETY
                     GTGRIRKTSEDGVVSGISFHISGNGVDKTVTTKEDGTVDIQLMPGTYTVTEQSIDRYL
                     PQDTQKITIASGQTSTVTFNNKLKRGSLEVIKSSEDDFVEGVTFHLFGTSLAGLPVDE
                     YAITDASGVAKFENVLISGNEPYTVEEVDTAIRYVVPDDQTAPIQWKQVTKRNFENIL
                     KKFRVEVVKTDHVTGYAQGDASLAGAVYGLYQGDELIASYTTDANGSFISDYFICDSN
                     WTLREISPSPGYLLDETIYPIPAEPGNFTVELNQIPMEVTEQVIMGRIRLIKHIDSEL
                     EDTESTPAAREQEAGMVMLAAVSVDDQPEEAVDGADVQTEETVSGNDAQTETENTEPE
                     VSDEEAQEPEESGTEESAVPEIEPETNPYEPDPVPMDEIEASGHEGIIEQPEKGAKFQ
                     IYLASAGSFENAREAERDELITDADGFAISKDLPYGRYVVHQTEGMEGQAFIPDFTVF
                     IRSNEQTYSYILNNLTQSSFIRVEKHDAETGKIIPAAGVGFQVRDLSTGEIISQTVYY
                     PTPVTIDTFFTNDEGWLMLPCELSYGQYELIEQETCFGYVLDSEPVPFTVDGTQDVVV
                     VEKHNMPQKGIIHITKTGEVFATVAEMDGVDKDGAEIMYQPVYEIRGLEGATYEIRAA
                     EDIYTPDGTLRAAKGDVVDTITTAGNGIASSKELYLGKYEICETEAPFGMVLNQESKQ
                     VELVYAGQEIDVTEVSSGFYNERQKVQMSLEKMLEVNEVFGIGNNNELTNVTFGLYAA
                     EDITAADGAVIPADGLIEVVGIDADGNGVVKTDLPFGSYYLREHSTDAHYILSDEKYP
                     VTFSYGSQEDAVITVKANDGEAIVNELIYGSVSGMKVDENGDALSGAKIGLFAPSVTE
                     FTEENAILVTTSAEDGAFSFNDIPYGKWIIREIEQPEGFVLCTEQFPVVINEDAQVIE
                     VEITNEFIRGNLHLTKFDKDYPENKLSGAVFEVYRDTNGNKELDKEDELLGVMEETEP
                     GQYEMKDLLYGGVLIREKTAPDGFYLDENTYYIMIDTDGNTYEVENEAGKGFYNQAFR
                     GNLKIVKTSSDGKVEGFSFRIVGDNYDKTFQTDANGEIFIENLRVGKYTVTEVEDSVS
                     AGYKHPDPVTVELVKDETLTVNVHNDKVTVDVPKTGDNFNPWIWVGLIAASTAGLGAS
                     IFWAKKRRKKMAETAE"
     gene            25946..26500
                     /locus_tag="ACG808_000023"
     CDS             25946..26500
                     /locus_tag="ACG808_000023"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045689.1"
                     /translation="MKIKLLKGALTVLSLICILCIPVTPIYAEPQGTDGTEMQVMEPE
                     KLEIQLGADWAGVEFQLKTDSGLYPGTIAVGEDGVLRMELGGSKSYVLTCMNSSVSAP
                     EATQAPATTEEAQNESNAGQESNESNSGQDTEGTVAGIPILHLVLFAGGMVLAVGSLV
                     AMRVMKKRRESDAEYEDEDEYEDD"
     gene            26881..27354
                     /locus_tag="ACG808_000024"
     CDS             26881..27354
                     /locus_tag="ACG808_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005333505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3268 family zinc-finger domain-containing
                     protein"
                     /protein_id="HFS5045690.1"
                     /translation="MKKQKKKRKGFQAGSMRCPYCGSPVIYRSADGIYHDNSKNTMLY
                     VCSHYPECDAYVRVHAGTNIPVGSLANHELRTLRRTAHHYFDQLHQSGMMSKQDAYQW
                     LADLICAPLSEAHIGYLGEYYCKQVIEESRRLLTRQKSGKNRCRFQALEGRVAAS"
     gene            27351..27905
                     /locus_tag="ACG808_000025"
     CDS             27351..27905
                     /locus_tag="ACG808_000025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS5045691.1"
                     /translation="MTLTEKERGKIEKNIGLVHKVIRDKLQPPYQVGMYFYEDLFQIG
                     CIGLCKAASTDKGGTFSTYAYRLIWHQICDAMIYATRRQTKETTCDVTPYIAAKQENP
                     EELSDFRMDIKRALEQAKADAPPSTRKGIDAICMMSKGYTSSEIGEKMDASAKLVCAW
                     VSKARKFLKSRPEFAQIAAAYHLE"
     gene            27984..28382
                     /gene="lepB"
                     /locus_tag="ACG808_000026"
     CDS             27984..28382
                     /gene="lepB"
                     /locus_tag="ACG808_000026"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFS5045692.1"
                     /translation="MNIRKKNIWIWLIPFLIMALTLILFKAVFLLGYVPTESMEPTLK
                     KDSYIVGTRIFSKLEIGDIIIFHHDGKLLVKRIAAEEGEAIEHNGVGLMVPEGCYYVL
                     GDNAEHSLDSRYWEYPFVKQKDIVARLFWP"
     gene            complement(28563..28877)
                     /locus_tag="ACG808_000027"
     CDS             complement(28563..28877)
                     /locus_tag="ACG808_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004056038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5045693.1"
                     /translation="MYEDFVPERLAKLRLQKGVSARDMSLSLGQANNYINNIENKKSL
                     PAMQSFFYICEYLGVTPREFFDEGNPYPEALQEFIEEGKKLDPKSMAYLLGIMKELNS
                     KK"
     gene            29027..29542
                     /locus_tag="ACG808_000028"
     CDS             29027..29542
                     /locus_tag="ACG808_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005361115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLOG family protein"
                     /protein_id="HFS5045694.1"
                     /translation="MKGKTCCITGHRDVPDKEINSIKKALHQEIKKAVKDGFTVFLSG
                     FADGVDQYFAEIVLELQKENRNLKLIAVLPYRDRVKGLNHRVQTRALLSACSEMVVIQ
                     EEYQPNVYLQRNRYLVEHSDRVIAVYDGREKGGTVKTIRFAHRLQKELREIPVGLNLD
                     KNRINLGHNNV"
     gene            29630..29977
                     /locus_tag="ACG808_000029"
     CDS             29630..29977
                     /locus_tag="ACG808_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003390711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04076 family protein"
                     /protein_id="HFS5045695.1"
                     /translation="MKKWYDEEYEFEIEVTGFLRGDHTERYCRNGEEVGDKYTCTYGC
                     PVNADGQGICSKVMIMLFPIMEAVRSGGDLENIGGSGKYCKEIVCPDGCVMFRLTAQK
                     LGNENFFKGKFFD"
     gene            30005..30418
                     /locus_tag="ACG808_000030"
     CDS             30005..30418
                     /locus_tag="ACG808_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015518012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HFS5045696.1"
                     /translation="MLDKIPNFDDMIALVGQSLYDVWQKLCSAIDEQYDMDCIWNSGG
                     KAWTYEYKYRRGGKTLCALYARENCIGFMVILGKDERARFEADRSEYSESVQNVYDDS
                     KTYHDGKWLMFEPVDTSLFGDFMQLLAIKRRPNRK"
     gene            30677..31045
                     /locus_tag="ACG808_000031"
     CDS             30677..31045
                     /locus_tag="ACG808_000031"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045697.1"
                     /translation="MKRELGIARCGLACCLCSENCSGCNSGECPDREWCENRKCSLSK
                     SIGHCYECEEECRKGLLAKIKPYGFTEFVKKYGEKELLDCLERNEANGIVYHRNGIMG
                     DYDDFDDVEKLMDFIKTGER"
     gene            31104..31538
                     /locus_tag="ACG808_000032"
     CDS             31104..31538
                     /locus_tag="ACG808_000032"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HFS5045698.1"
                     /translation="MSEKFPQEAKKIMDERFGHDTLLSLATTDQNIPYVRTVNAYYED
                     GCFYIITYAASNKIKQIGNNPIVAVAGEWFTAHGKGINLGWFCKKENHEMAQKLRQAF
                     SEWIDNGHNNFDDENTIILCVQLTEGILFSHGTRYDIDFAGN"
     gene            31600..32022
                     /locus_tag="ACG808_000033"
     CDS             31600..32022
                     /locus_tag="ACG808_000033"
                     /inference="COORDINATES: protein motif:HMM:NF024076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HFS5045699.1"
                     /translation="MLDLKDINYMPDIFEISDYIGNPLFDQFCEFMDREYKALRKIEY
                     SKDVWARGWNIKLRKAGKSLCVIYPKENYFTVLVVIGNKEKEKVEGILSQLSNEVQTI
                     YRETKEGNGQRWLMIELHSNDCIYQDTLKLIRIRRESK"
     gene            32053..32658
                     /locus_tag="ACG808_000034"
     CDS             32053..32658
                     /locus_tag="ACG808_000034"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003646004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HFS5045700.1"
                     /translation="MTELEKCMAGELYNCHDEIFLQYKKQAKELLDIYNRLTYDQKEE
                     KKGILHKMFGGIGTNVSVGAPFLCDYGCNIITGSNVSINMNCSFIDCNQIKIGNNVMI
                     ASNVQIYTATHPVELAERLTPDWTPESGKHFCQVYALPVEIGDGCWIGGGVIILPGTK
                     IGAGSVIGAGSVVTRDIPPNCVAVGNPCRVIRRINGEPKHD"
     gene            32651..33319
                     /locus_tag="ACG808_000035"
     CDS             32651..33319
                     /locus_tag="ACG808_000035"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5045701.1"
                     /translation="MIKLVAFDLDGTIGDTIPMCIKAFRQAVSPYAGHLLTDREVVET
                     FGLNEIGMIKSIVPEKWKQALCDFYFLYEKLHHECSEPYEGIPDLIAALRAKGFILAL
                     ITGKGERSCRITLNEFGMEHDFDEILTGSEHENVKAQSLKLLLKKYDLLPAQCLYIGD
                     AVSDVSEAGKAGVICLSALWGTTAKAEELKSINADYIFETVGELKNYLMKNTNHEPEE
                     VSIP"
     gene            33316..33864
                     /locus_tag="ACG808_000036"
     CDS             33316..33864
                     /locus_tag="ACG808_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HFS5045702.1"
                     /translation="MKGFNRQNQLFSLCGLNCGLCPMFLNKYCPGCGGGEGNQSCKIA
                     RCSMEHNGVEYCFQCDEYPCPKYEHIDDYDSFITHGNRKVDMKKAHQIGMEAYNAEQE
                     EKARILDILLADYNDGRKKTLFCVAVNLLDLQDLQAVLRQIENRADLDTLTLKEKSAF
                     AAGLLQEAAAKNNIELKLRKKK"
     gene            34009..34770
                     /locus_tag="ACG808_000037"
     CDS             34009..34770
                     /locus_tag="ACG808_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS5045703.1"
                     /translation="MDKWFTIDQIDEDTYIISEYRHWEETHCYLLNGSSRSLLIDTGL
                     GICNIYDEVMKLTNKPITAVATHIHWDHIGGHKYFPDFYAHAEELSWLNGEFPLTIET
                     IREMVADRCDLPDGYDTGTYEFFQGTPTKVLEDGENIELGGRNIQVLHTPGHAPGHMC
                     FWESDRGYLFTGDLVYKDTLFAYYPSTDPDAYLASLEKISELPVKKVFPAHHSLDIQP
                     EILSRMRNAFRQLREDGKLHHGSGTFDYGDWAVWL"
     gene            34868..35332
                     /locus_tag="ACG808_000038"
     CDS             34868..35332
                     /locus_tag="ACG808_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5045704.1"
                     /translation="MKYVCTVISVADISAARKFYEELFGLEVYQDYGKNIAFTCGLAL
                     QQDFDWLVNIPKEKVLKKSNNAEVVFEEQNFDGFLNKLKEYSEIEYLGEVIEHSWGQR
                     VIRFYDLDEHLIEVGEDMQMVVKRFLASGMTMEEVSAKMDVSIEDLTKLLNS"
     gene            35383..35793
                     /locus_tag="ACG808_000039"
     CDS             35383..35793
                     /locus_tag="ACG808_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HFS5045705.1"
                     /translation="MKMPEKIDISMFAPCGMNCKVCYKHCYHKKPCAGCLNSDKGKPE
                     HCRKCKIKDCIKEKALSHCFECAEYPCKLIKDLEKSYNKRYQASLIENSRFIQEQDLE
                     HFMEQQKIKYTCSKCGGIISIHDRECSECKEKMK"
     gene            35823..36278
                     /locus_tag="ACG808_000040"
     CDS             35823..36278
                     /locus_tag="ACG808_000040"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFS5045706.1"
                     /translation="MLLTYREATKSDANLLINIYNSSFYEDYIHYGECPGYGKTKEQM
                     ELSIIKFPKYIIIKDSIPIGVISFENKGNGNYYLGCLCVIPMYQRMGIGSQAFQYMLS
                     ICPEWKQITLVTPSNKEQNIKFYTEKCGFNTDGEEMDGNVEVTKFIMKR"
     gene            36508..37113
                     /locus_tag="ACG808_000041"
     CDS             36508..37113
                     /locus_tag="ACG808_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045707.1"
                     /translation="MKEIINFIEANVDGKTLFTKELVYELENGVLQGVYSDQISFSNL
                     KYSQSGFQLDMFIVSNEKIWLMGKDGEREKLRKDFSGVSLFRFELAKRKSTNSLTGCF
                     RFISASGKNVAAEAIVSGIYDVRLENDVLKLSEDQVLYRDQPIQEGNFKPVAFQSEHR
                     FYVKANKLHYEYNGKCFDVDAKTMRRNDSSDTFPPFISIEK"
     gene            37254..37604
                     /locus_tag="ACG808_000042"
     CDS             37254..37604
                     /locus_tag="ACG808_000042"
                     /inference="COORDINATES: protein motif:HMM:NF024087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HFS5045708.1"
                     /translation="MINKKMIAFCGTYCGVCEWKDKIGCKGCKANRGIMFWGKCDKAI
                     CCIEKGLEHCGECSDMPCQKLRDLFDDPEHGDHGARLRNLKNWKDGICTYEKLGNTAQ
                     EKAKNLKTIDNTND"
     gene            37666..38289
                     /locus_tag="ACG808_000043"
     CDS             37666..38289
                     /locus_tag="ACG808_000043"
                     /inference="COORDINATES: protein motif:HMM:NF045597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3072 family TudS-related putative desulfidase"
                     /protein_id="HFS5045709.1"
                     /translation="MEIGKSDWNNHTLIFGLIIKICEFVRVYTMFNDGRSKKVVFVAH
                     CFLNQNSISDGTAIYPAANKDVVDFFLNADIGIVQMPCPEFCCLGLDRGNIHGADSPV
                     VVENTRIRSAMQNDESNLKLTRLADYVVQHIMEYKKYGFEVIGIIGANRSPNCGVETT
                     SDNDAEINGMGLFIDKIFRRLLRENISIPMIGIKGTDNIQEKLQQLI"
     gene            38375..38866
                     /locus_tag="ACG808_000044"
     CDS             38375..38866
                     /locus_tag="ACG808_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside adenylyltransferase"
                     /protein_id="HFS5045710.1"
                     /translation="MDKKETATKEDLMAVVSLFENMGILYWVDGGWGVDLLAGKQTRD
                     HRDIDINFDAQYTERLLDILSEHGYKVETDWKPVRIELYSDEYGYLDIHPFVLNDDGT
                     SKQADLEGGWYEFEKDYFGSAVFEGRTIPCISLKGQKIFHSGYELRDKDKHDLSVLES
                     ISE"
     gene            38885..39433
                     /locus_tag="ACG808_000045"
     CDS             38885..39433
                     /locus_tag="ACG808_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HFS5045711.1"
                     /translation="MEEATRYIALLRGVNISGKNKVPMAELKKIFEELGFGAVKTYLN
                     SGNVIFSSDEENIGSLTDRIETTIKKWFGLDIPIFVISKEDLEDILQHAPAWWGDENK
                     EIYDNLIFIMPPATFAEVWGEIGEPREGLEKIKNYKAAVFWSFSRKDYQKTNWWRETA
                     SANISGKLTIRTANTVRKIVGM"
     gene            39469..39618
                     /locus_tag="ACG808_000046"
     CDS             39469..39618
                     /locus_tag="ACG808_000046"
                     /inference="COORDINATES: protein motif:HMM:NF024050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HFS5045712.1"
                     /translation="MQNNELLNHLDKLHTTELGVERIKRNLALDTDHVVDWCRNMIPS
                     VEASM"
     gene            40105..40716
                     /locus_tag="ACG808_000047"
     CDS             40105..40716
                     /locus_tag="ACG808_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5045713.1"
                     /translation="MPKGIMLTPEQQEERREEIISVALQLIEKNGFQKTSMREIAILA
                     NMGKSSLYDFFKTKDEIVVYAVEKEIEETIKKVHRIIADESSPEQCLRKIMLNHLGVP
                     KQYRTVLMWLNTESDYLEEDYRKRLKTARYTYQDIIKSVIENGVESGVFRKTDADLVA
                     RLLINSIISIIYTSRPSASPEKMLDETMDIFLHGIMKDGGELL"
     gene            40713..43280
                     /locus_tag="ACG808_000048"
     CDS             40713..43280
                     /locus_tag="ACG808_000048"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HFS5045714.1"
                     /translation="MIYYTLPLLHKQATLEMVGGKGMSLSKLLTAGIPVPEGFHVTTA
                     SYKIFVEKNHIQPHINKLLDGIDSNNTSQLENVSTQIGMLFHNGEMPQEVSDAIKTAY
                     AGLGNIAVAVRSSATAEDLPDASFAGQQETYLNIQGENEVLDAVKRCWVSLWTARAIA
                     YRVKNGIKQEIVALAVVVQKLAFSDASGVMFTLNPINGRRSEMIVNAAWGLGESVVSS
                     LVTPDTIVVDKNAERIVSYEAANKEIMTVRTSDGTEEIPTPKQLRKKHALTRNQVMRL
                     TQLGKKIEKYYEMPMDVEWALEKDKLYIVQARPITVLPPEWTLPEKDVIYTKGSLAEH
                     LPNPVTPLFATLGLEIVNRASALLWIDMFGKSAKKLLPENGAYTIINGYVYLSANSKP
                     FLIAVKSLSPRSLRRALTNSVARWETARKEFEDVIKQWEEKPMHMLNAHQIMEGIQTV
                     FYGACIYFTRIQLTLPAASISETLFTKFFQGAARRAGITDTSVLLLGFDTIALQSEKN
                     LWDLSEWAKQNNTLGFYLKSNPATKIAEDFKSSILPAEVSQEVWIEWKSRINAYFKEF
                     GCTAYEFDFAYATPQEILTPTFESIKAFLEEKGENPYSRQIAFEKRRKQAENEILQQI
                     GGHRKKLFLKLLHWAQNTAPMRENAIYLMGMGHPLIRRMLQEISERLLTGGAISHLDD
                     IYWLTKTELETLITQLDKNIPLSNLSEAIPARQAEHDTFRGYVSPSQLPEKNKKAVSH
                     ATQIQKDGKIVLTGIGTSAGVVTAPACVLNSPADFEKFQPGSILIAVTTTPAWTQLFA
                     SASAIVTDIGGPLSHSSIVAREYGIPAVMATHTATRSIKNGQMITVDGSEGTVTIDE"
     gene            43273..44433
                     /locus_tag="ACG808_000049"
     CDS             43273..44433
                     /locus_tag="ACG808_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5045715.1"
                     /translation="MNKKPIFALKLATLVIALGYSLILPVMPFYMENLGAGGRELGWL
                     TAVYALAQTVCAPFWGALSDRIGRKPIISIGIIGYSISLFLFGLASSFWTLFIARSLS
                     GILSSATSVASLAYVGDSSSEEERSKNMSQLGASMSVGVMMGPLLGGILAGYSLALPF
                     FTGAGISFIAFLLILTLLPESIERSGQTEKRKPFDFSDLKSIIFGSAGMVLILIFVVN
                     FCQTGLQGITGLYVVDKFGFSTKQVGVIWMALAGILIVVQGFLTGPLAKKIGEKRLIL
                     IGMFCKALVAFAMVLTTGFVSVLVIFGLFAVSSAITVPTLNATLSKTDNQHKGTLMGF
                     ASTAGNLSKVIAPLLTGYLYESNIELPYITTGGIALIGVVICYIWIKNQKNK"
     gene            44643..46028
                     /gene="mgtE"
                     /locus_tag="ACG808_000050"
     CDS             44643..46028
                     /gene="mgtE"
                     /locus_tag="ACG808_000050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFS5045716.1"
                     /translation="MNLKKLEFEKLIEQISTLYREKKYSQLKSILADINPTDIAVLFD
                     EFPNEQRILLFRLLPKEEAAETFVELDGETQEALIHAFSDTELREVIDELYLDDTVDI
                     IEEMPATIVKRILRNTDAQTRKSINTLLKYPEDSAGSIMTPEFVDLKKDNTVEDAFKR
                     IRRTGVDKETIYTCYVTDSSRHLIGVTTVKELLLHDYGDKIEEFMETNTIFVNTHDDK
                     EIAANFLDKYNFLALPVVDMENRLVGIITVDDAIDVLQEENTEDIQKMHAIMPNEKPY
                     LKTGIFETWKSRITWLLFLMISATFTSMILNAFEDKLSALIVLTSFVPMLTGTSGNSG
                     GQASAVIIRALSLKEIDFKDISKVIWKEFRVGVLCGITLAAVNFVKIWFVDRTLLGMS
                     AITIQVDLVISLTLIIEIIFAKMVGCVFPILAKKMKLDPAVISSPFITTVMDALSLLI
                     YFWLATMILHI"
     gene            46456..46713
                     /locus_tag="ACG808_000051"
     CDS             46456..46713
                     /locus_tag="ACG808_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HFS5045717.1"
                     /translation="MQNNELLNHLDKLHTTELGVERIKRNLALDTDHVVDWCRNRISS
                     VEASISRKGKNWYITVDGCIITVNAYSYTIITAHKVQEQQR"
     gene            46749..47474
                     /locus_tag="ACG808_000052"
     CDS             46749..47474
                     /locus_tag="ACG808_000052"
                     /inference="COORDINATES: protein motif:HMM:NF025502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4304 domain-containing protein"
                     /protein_id="HFS5045718.1"
                     /translation="MTSREKKNKVIQQVIKPALKEAGFHSLRGTQAFSIHKEACTIAV
                     NIQSSQFNSNVTGFSFWLNIVVDTPDLSDEQLQDVTFFKSFTEACFLPHQGRLHPLRD
                     IRGYVIDGYKNYKPMDTPYEKIQAIIANDMQQYIIPGLSEIDSIADYQECVALWQEES
                     KSQFVRLVDYFSEAHMLTLPFQKDTWIGTVNMKELVEKKKTLCLTTDEIMQNKSIYCK
                     VRELSTHPQEDTWPLICMTLSAE"
     gene            47701..48516
                     /locus_tag="ACG808_000053"
     CDS             47701..48516
                     /locus_tag="ACG808_000053"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS5045719.1"
                     /translation="MDHSNSKIVFDEKLNIEVLTLTGMIESLPPHFHDYYELGYIESG
                     SRRVICQGKEYTIGKNDLLLFNPKDNHTCLELKKELLDFRCFHITTERMEELTLECLG
                     FAICPYFEPQIVYQSDLIPQITELIDLIENGSYCDLQKEELLYMIIGDLVADYSQPLE
                     CEQIEASDIVERACEYIERHYASNISLCDLSTFTGFSKYHFIRMFTREKGISPYRFIE
                     CTKMTEAKKMLKAGEDLSNITYQLGFSSQSHFTNFFKKYAMVTPKQYSKLYNA"
     gene            48627..49070
                     /locus_tag="ACG808_000054"
     CDS             48627..49070
                     /locus_tag="ACG808_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008689502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFS5045720.1"
                     /translation="MLLNVQQRQALIKYLLSQEKKDTDSADSPCDSFQIHDPQAEVGG
                     VFTEIEMGELYFCLEQRLVRVRGQVIELTAKEFDILALLLTHPKRVFTYELIMELVWN
                     EDYTFYSKKAVSNHMSNLRKKLKVTPDALDYIKNIVGVGYKFEAP"
     gene            49244..49942
                     /gene="vanR-D"
                     /locus_tag="ACG808_000055"
     CDS             49244..49942
                     /gene="vanR-D"
                     /locus_tag="ACG808_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-D"
                     /protein_id="HFS5045721.1"
                     /translation="MNEKILVVDDEKELADLVEVYLKNDGYTVYKFYNGKDALKCIES
                     VELDLAILDIMLPDVDGFQICQKIREKFYFPVIMLTAKVEDGDKIMGLSVADDYITKP
                     FNPLEVVARVKAQLRQYMRYKQPSLKQEAECTEYDIRGMTISKSSHKCILFGKEIQLT
                     PTEFSILWYLCERQGTVVSTEELFEAVWGERYFDSNNTVMAHIGRLREKMKEPSRNPK
                     FIKTVWGVGYTIEK"
     gene            49932..51077
                     /gene="vanS"
                     /locus_tag="ACG808_000056"
                     /pseudo
     CDS             49932..51077
                     /gene="vanS"
                     /locus_tag="ACG808_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_028530133.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS"
     gene            51177..52268
                     /gene="vanY-D"
                     /locus_tag="ACG808_000057"
     CDS             51177..52268
                     /gene="vanY-D"
                     /locus_tag="ACG808_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_049898469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transpeptidase-like D-Ala-D-Ala carboxypeptidase
                     VanY-D"
                     /protein_id="HFS5045722.1"
                     /translation="MWKGVKRNVERQNNNENQYGRNRRKDKRRKLFFYRAACAMLGLL
                     IVCVIFGAVYFLRESKDPALPSKENTKTSKDYSFLADGQSEEESPASEPAISNRANAI
                     DLNIIAANAIVVNKDTDALLYQKNGTDRIAPASTAKMITALTVLEYCSPEDEMRVGAE
                     IEMIHSDSSTAWLMKGDTLTVRQLLIALMLPSGNDAAYTLAVNTGKTIAGDNSLSNQQ
                     AIQIFMDKVNEKAKAIGVIDSNFVVPDGYDAEGQYTTAYDLAIIAKACLEDPIISEIV
                     ASNTSYERWPNGREVTYNNSNELLDPNSPYYRPEVIGLKTGTSSLGGACVVSAAVIDG
                     ETYICVVMGSTKESRFQDSVDILDKIKAR"
     gene            52288..53259
                     /gene="vanH-D"
                     /locus_tag="ACG808_000058"
     CDS             52288..53259
                     /gene="vanH-D"
                     /locus_tag="ACG808_000058"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023043160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-D"
                     /protein_id="HFS5045723.1"
                     /translation="MNKKMDITVFGCESDEMEVFQKISYELGVTATLIKDSISESNAG
                     LADGCCCVSVSHKAELSEPILLALKNAGVKYISTRSIGFNHIDIQAAGLLGMVVGTVE
                     YSPGSVADYTVMLMLMLMRGTKSILRETQRQNYCLNDLRGKELRDMTVGVLGTGRIGQ
                     AVMERLKGFGCKVLAYDRNQKAGTDYVSFHELLKKSDIVTLHIPLAEDTRHMIGCEEL
                     EMMKQEALLINTGRGALVDTAALVEALKGQKIGGAALDVLEGEEGIFYHDCTQRTIEH
                     PFLSVLQGMPNVIVTPHTAYHTDRVLVDTVSNTIRNCLNFERSLGNV"
     gene            53252..54283
                     /gene="vanD"
                     /locus_tag="ACG808_000059"
     CDS             53252..54283
                     /gene="vanD"
                     /locus_tag="ACG808_000059"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023043159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanD"
                     /protein_id="HFS5045724.1"
                     /translation="MYKLKIAVLFGGCSEEHDVSVKSAMEVAANINKEKYQPFYIGIT
                     KSGAWKLCDKPCRDWENYAGYPAVISPDRRTHGLLIQKDGGYESQPVDVVLPMIHGKF
                     GEDGTIQGLLELSGIPYVGCDIQSSVTCMDKSLAYMVVKNAGIEVPGFRVLQKGDSLK
                     AETLSYPVFVKPARSGSSFGVNKVCRAEELQAAVTEAGKYDCKILVEEAVSGSEVGCA
                     ILGNENALMAGEVDQIELRHGFFKIHQEAQPEKGSENAVIRVPAVLPDEVRERIQKTA
                     MKIYRILGCRGLARIDLFLREDGCIVLNEVNTMPGFTSYSRYPRMMTAAGFTLTEILD
                     RLIELSLRR"
     gene            54289..54897
                     /gene="vanX"
                     /locus_tag="ACG808_000060"
     CDS             54289..54897
                     /gene="vanX"
                     /locus_tag="ACG808_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023042861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX"
                     /protein_id="HFS5045725.1"
                     /translation="MKKNFAFLDEMIPGIRWDAKYATWDNFTGKPVDGYMVNRVMGTK
                     ELGVALRKAQKMAEKLGYGLLLWDGYRPQCAVDCFLNWTSQPEDNLTKKRYYPNIKRN
                     EMVAKGYVASKSSHSRGSTVDLTIFHLNSGMLVPMGGDFDYMDERSHHTASGLSEEES
                     KNRQCLRYIMESSGFEAYRYEWWHYVLVDEPYPDTYFDFCIA"
     gene            55058..55969
                     /locus_tag="ACG808_000061"
     CDS             55058..55969
                     /locus_tag="ACG808_000061"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5045726.1"
                     /translation="MKSNRMFGILCILLERKKITAQELAEYFEVSVRTIHRDLLDLSS
                     AGFPVTTQQGIGGGISLLPNFKYSKSALNKEDIDLIVSGIQGFASIDESSKIKTLLAK
                     LRLGQEDKLLLENDIIIDFTSWNHKSTTIEKIKIIRSAIASRRLLELEYYSSNGYSKR
                     TVEPYKLIFKEEYWYLFAYCTQRQDFRVFKLNRISKLLLCEQIYTERTDYEIPVLQSA
                     FSNGVGQLVTVRMDKSYEFLAIDFFGQSNIREENNSLYISFYTEYPEWIVSTFASLGD
                     KAEIIEPKTLRDDIKAFLEQARKQYDK"
     gene            56112..56753
                     /locus_tag="ACG808_000062"
     CDS             56112..56753
                     /locus_tag="ACG808_000062"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5045727.1"
                     /translation="MTDTKEKILAVALQLFAKDGYEAVSVSKIAEKLGITKGALYKHY
                     ENKKDIFNSIVARMSQSDHEIARKYGVPENVFDCAPEQYEKIGLKNLKNFAVSQYRFW
                     TSDEFAADYRKMLTLEQYRNPELNKLYQDSLVRGPVLYLKDIFREMIRCGVLKENDPY
                     QLAIEFFSPLFLLINMSDENESYVDIEKRLIKHIDFFIESHTKERENKNEIYS"
     gene            56737..57453
                     /locus_tag="ACG808_000063"
     CDS             56737..57453
                     /locus_tag="ACG808_000063"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HFS5045728.1"
                     /translation="MKYIRSQKYNIPELQAKIMGPNPVKLEEELLLHCKISQNAIVCD
                     LGSGQGLTSLFLVKEYGFTVYATDLWSDPEDNRKFFRKMGLSDEQIIPVKADATALPF
                     EEEFFDAIISTDSYNYFGRDPKFLDDKLLPYLKHGGYVYIAIPGMKKDCHSNLPPELL
                     LSWTPDQLDYMHDIMYWENIIRHCKGARVISIHEMESNEEVWNDWLKQENEYAISDRK
                     AMEAGGGKYLNFISIVLQRL"
     gene            57782..58225
                     /locus_tag="ACG808_000064"
     CDS             57782..58225
                     /locus_tag="ACG808_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008689502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFS5045729.1"
                     /translation="MLLDARQRQELVNYLLGKEKQNGGSADILCIRSGAYELLTENEG
                     IFTEIHMGDLYFCLEQRLVRVDGQVVELTAKEFDILALLITHPQRVFTYELIMELVWD
                     EAAACYSRKAVSNHMSNLRKKLKITPDGLEYIKNIVGVGYKFEIP"
     gene            58417..58794
                     /locus_tag="ACG808_000065"
     CDS             58417..58794
                     /locus_tag="ACG808_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5045730.1"
                     /translation="MEIIISSNTSKPIYEQITSQIKAMIMSGELQTGDPIPSMRALAK
                     SIHVSVITVQKAYEDLQRDGFIETTVGRGSFVAAQNKDFYQEEQQRLAEEHLQEAADI
                     GRTSGISLGKLVELLTMFYQEEE"
     gene            58797..59657
                     /locus_tag="ACG808_000066"
     CDS             58797..59657
                     /locus_tag="ACG808_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005933713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5045731.1"
                     /translation="MDTILQVENLTKQYEGFQLDHVSFDLPKGTIMGLIGENGAGKST
                     TINAILDLIKKDDGTVTFWGQELSSSKQLKEDIGVVFDGVNFYETLTPAKVGKISGSA
                     YKQWDEYLYRDYLKRFGLPLDKEIKTLSKGMKMKLCIAVALSHKPKLLILDEATSGLD
                     PVMRDDILDVFLDFVQDENHSILMSSHITTDLEKVADYITFIHQGKVLFCKTKDELRY
                     KYGIIRCGAAVFDQIDKAEILAYRKDDYQWNVLVADKEKARKKYKAAVVDDATIDDIL
                     LLYVKGERAK"
     gene            59654..60301
                     /locus_tag="ACG808_000067"
     CDS             59654..60301
                     /locus_tag="ACG808_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HFS5045732.1"
                     /translation="MKSLILKDLYNIGHNTKSMLFILAVFAVAFIPTSGVGAYIFICG
                     ILCSMMIVTTFSFDDNSKWARYAMIMPISKRDLVIGKFIVLAIFCAIGSVFGLAVGLI
                     GGGIAGKITFSPAAIVELLFLTLAATVISLIFGSMSISLVFKFGAEKGRVLLLVSFLI
                     PAAICFGAYKLLAILGVELTDQKVFILLCCSPLIALVWSYGMYQISYRIFLKQEL"
     gene            60318..60503
                     /locus_tag="ACG808_000068"
     CDS             60318..60503
                     /locus_tag="ACG808_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HFS5045733.1"
                     /translation="MKKYEYVNIEYSAKGVMFSYVEKHREIIDSYAEKGFRYVGFVPT
                     EVGANGCIRKIDLVFEK"
     gene            complement(60867..61469)
                     /locus_tag="ACG808_000069"
     CDS             complement(60867..61469)
                     /locus_tag="ACG808_000069"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5045734.1"
                     /translation="MPRQFKQARLINKLKMTEAAEKLGISQPTLSAWEGERKSPSIDG
                     LEKMSVLYGVTTDFLLGRSEQGISVQSVPIAPETLPIFHGKPVWSAEYGWMLVDAGNH
                     TLLLPNGQTVPFADAKRLFTLPQLFSEPSLPSGKPLILSEIQQFTRIWLEPISPDSNL
                     RTELRGWYQVKKHFVQNEYGNRFYLDTYRAKWLAFHPEQQ"
     gene            complement(61483..62844)
                     /locus_tag="ACG808_000070"
     CDS             complement(61483..62844)
                     /locus_tag="ACG808_000070"
                     /inference="COORDINATES: protein motif:HMM:NF016232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5045735.1"
                     /translation="MPEMQIIEWKEIWKDDYLAWICGFANAQGGTLYIGKNDNGETVG
                     LSNSHKLLEDLPNKIRDAMGIIVPVNLHTDGETEYIEISVQPYPIAISYKGVYYYRSG
                     STNQKLTGLELESFLLRKQGATWDNLPLPSFTISDIDNGVVKRFIKWAAKKGRIDESA
                     LDEQKETLMKKLHLMNNDYLTNAAMLLFANDPEEWQLGAFTKIGYFESDSELRYQDEI
                     HGSLLDQIDRIIETIHLKYMKAKITYEGMQRIERYFVPDAALREALLNALCHKDYARG
                     IPIQVSVYNDRLYIANCGRLPENWSAEKLMEKHSSEPFNPNIAHVYYLAGFIESWGRG
                     IEKICDACREDGVPLPEYDITGNSVMIKFIAPKDRIVYGPGETANGTVNGTVNGTVNL
                     SDKEKEILALLSEDPAYTYQNLADKLNIGRKAVFGRIKNLKEKGIIERIGSDKNGYWQ
                     INE"
     gene            complement(62837..63043)
                     /locus_tag="ACG808_000071"
     CDS             complement(62837..63043)
                     /locus_tag="ACG808_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFS5045736.1"
                     /translation="MKFISTTEAGKKWGLSSRRVGILCSEGRIPGTQKTGNTWLIPED
                     AEKPADARIKSGKYIKKCEEQSNA"
     gene            63266..64123
                     /locus_tag="ACG808_000072"
     CDS             63266..64123
                     /locus_tag="ACG808_000072"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5045737.1"
                     /translation="MYEEVGISSNNVPITLSIWYTERRSPTIVFLPGTMSHPLMYENF
                     LCGLSERGFNIIGVHYLSHGKSPKIKKNYTMEDMLHNVYDATTYGIENFGENIAVMGS
                     SQGGILAAMAAGKDTRLKAVFPHNIMLTTLKETMSLTKYPAWTHRFMGLIQWGFRVGG
                     KLLPNYKVPGDAYLDYDRVFYSEQAKQDCLNDPMLLPYYPLRFVASLFNADLSCLENG
                     SIQCPVILITAKGDPLFSFDYTSKVFDLIHAPQKELLTLELNRHMIFNEDTDTTINAL
                     EPTLHKYLG"
     gene            64250..64783
                     /locus_tag="ACG808_000073"
     CDS             64250..64783
                     /locus_tag="ACG808_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5045738.1"
                     /translation="MIEGEKLFALHGLKKVTVDDLVAAVNISKGSFYAFYPSKEHLYV
                     EINFRLQKELFESIETTIKKKKYKNHRDLAKDAIKLSLTGLITSPILSQIDLSIMDYL
                     QRKLSPDIFESHMHNDIRILEILEDLGVVFLVPHTVIIKSLYSVLSCLEQFKEDEELN
                     MIQNLLVNGIIQQVVAE"
     gene            64886..65731
                     /locus_tag="ACG808_000074"
     CDS             64886..65731
                     /locus_tag="ACG808_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5045739.1"
                     /translation="MIDVKKLYFSYTEKPFVEDVSFHVGRGEIFGFLGPSGAGKSTIQ
                     KVLTGLNTKYKGSVKVAGIEIRERTNRFYENIGVDFEFSTCYEKFTARQNLAYFASLY
                     EKQPRSIDELLHMVGLENDGDKKVADFSKGMRSRLNFIKALIHDPDILFLDEPTSGLD
                     PTNNRLMKDIILAEKKRGKTIIITTHNMYDATELCDQVAFIVAGKVSALDSPHNLIMS
                     RGAAKIQYTYYDNGEKTGECLLDRTTEDKMLKNLISENRLLSIHSSEPTLNDIFVDIT
                     GRTLQ"
     gene            65728..66411
                     /locus_tag="ACG808_000075"
     CDS             65728..66411
                     /locus_tag="ACG808_000075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045740.1"
                     /translation="MRLGRLIRGDIHFQWKYGFYFIYFILTVLYVCGIAALTGHWKTD
                     IASIMIYSDPAAMGLFFMGAIVLLEKSQKVLNAMVVSPVKVSEYILSKTVALIAISTI
                     IALILGFVSGSNHLLGITVGTALTSAIFTMIGIIAATKISNLNQFLIVIMPIEIVCFV
                     PPIVGLFVKLPYLFRFFPFTACMNLITGKSVLLSPDTVLVIATLMILYMVARHTVEHM
                     WKSLGGVKL"
     gene            66408..67106
                     /locus_tag="ACG808_000076"
     CDS             66408..67106
                     /locus_tag="ACG808_000076"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045741.1"
                     /translation="MKKILSSTIQIFKQIKSDPMMFAACFSPFIMGTLIKFGIPFLER
                     ITKFSLQAYYPIFDLLLSIMAPVLLCFAFAMITLEEIDDKVSRYFSITPLGKSGYLFT
                     RLGVPSIISAVIAFIVLLFFSLEKLSVGMTVCLALLGSVQAIIVSLMIITLSGNKLEG
                     MAVTKLAALTLLGIPAPFFIDSYYQFAVGFLPSFWVAKAMQSEAFLYFSIGFVVALVW
                     YYFLTKRLFRKLAG"
     gene            67118..68029
                     /locus_tag="ACG808_000077"
     CDS             67118..68029
                     /locus_tag="ACG808_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFS5045742.1"
                     /translation="MEKGRLLQNENINKAIQVSKITLIINLGLSLLKFAAGYIGKSSA
                     MLSDAVHSASDVLSTIVVMVGIKISEKQPDKEHPYGHERMECVASIILSVALAITGVG
                     IGYSGIKKIFSGQYNTLSIPSGIALTAAVLSIVIKEWMYWFTRNAAKHTNSDALMADA
                     WHHRSDALSSVGSLIGILGARLGFAILDSIASVVICGCILKAALDIFKESINKMVDHS
                     CDNVTETKIREVVLKQQGVDGIDELKTRMFGAKMYVDIEILADGNLALYDAHGIAEAV
                     HQTIENNFPQCKHCMVHVNANELLYST"
     gene            68081..68221
                     /locus_tag="ACG808_000078"
     CDS             68081..68221
                     /locus_tag="ACG808_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045743.1"
                     /translation="MEQYWWIIIVVVLIVVGLLKLVIMKKYNQKRNSNNRSKDSEDDK
                     MY"
     gene            68478..69254
                     /gene="srtB"
                     /locus_tag="ACG808_000079"
     CDS             68478..69254
                     /gene="srtB"
                     /locus_tag="ACG808_000079"
                     /EC_number="3.4.22.71"
                     /inference="COORDINATES: protein motif:HMM:TIGR03064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0045184 - establishment of protein
                     localization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HFS5045744.1"
                     /translation="MKKKKIICIMLILIFTSGIVIGAIQLYKQYHEYSEGEDSYTDLE
                     DYVKLPEEPEAPSASPVDDETESGGREWPAVDFASLQEINPDIVGWIYIEGTEINYPV
                     VQGNDNQYYLKHLFSGEWNGSGCIFLDSRNRVDFSDRHSIIYGHHMKNGSMFSGLTEY
                     KKQEFYDSHSVALLLTPDKNYEIEIFAGYVASVQDKAWELAFPSDPDFTGWLDKAKER
                     SCFTSGITPAVTDRMLTLSTCSYEFNNARFVLLGILKPEE"
     gene            complement(69370..69570)
                     /locus_tag="ACG808_000080"
     CDS             complement(69370..69570)
                     /locus_tag="ACG808_000080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045745.1"
                     /translation="MMLLDIFAFLRILGQQKGFGNRFTIVLLQVVENHLSTCKPIAAK
                     LPQLFRSTSIKIVRLDHYGNET"
     gene            complement(69728..70153)
                     /locus_tag="ACG808_000081"
     CDS             complement(69728..70153)
                     /locus_tag="ACG808_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS5045746.1"
                     /translation="MEVIIKINGQALSVEVSVEVYEFLDRADHKEENLSHEQRRHWDI
                     REFDEYIVAAEDRTNQETPEQYVCRRETLDELLSMLESCTATQRRRFLLYALDGLSYE
                     QIARLDGCSKYTVRDSVEAARKKFQDFFKNRPHETRFSG"
     gene            complement(70664..71002)
                     /locus_tag="ACG808_000082"
     CDS             complement(70664..71002)
                     /locus_tag="ACG808_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003509323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5045747.1"
                     /translation="MKSSITSILVGVIFIGVFVTLFVLIVRAFLKYLKSSNVRKEKAA
                     VSQSLGETLKAYRTHCQMTQEFVAETIGVSRQAVSKWENGTSDPSTSNLFALAKLYGI
                     SVEELIKDVK"
     gene            complement(71117..71533)
                     /locus_tag="ACG808_000083"
     CDS             complement(71117..71533)
                     /locus_tag="ACG808_000083"
                     /inference="COORDINATES: protein motif:HMM:NF025645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4368 domain-containing protein"
                     /protein_id="HFS5045748.1"
                     /translation="MVRAIKPLNEFVEKILVHERARKGSIETTQEVEIYFNFVGRYVP
                     PHFGEVNLTPEEQEALRKKEERKNRLHQNYLRRKANGKQREYEDRIKTEKRRQMEAKK
                     AALRAEDMANGVYALTSSLQRKEPQKGILSARSAVI"
     gene            72019..72390
                     /locus_tag="ACG808_000084"
     CDS             72019..72390
                     /locus_tag="ACG808_000084"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045749.1"
                     /translation="MILYLDARTTVKDLIIDYIEVELANGETASLNWDESDIGRADDG
                     FSARYKGVYFGEVYANGRLEQLQDMKITDIGLYSESDTPLNICITSMEFEDDGRLLAF
                     EAPILHGNIVYQNESGEVIAC"
     gene            72384..72704
                     /locus_tag="ACG808_000085"
     CDS             72384..72704
                     /locus_tag="ACG808_000085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045750.1"
                     /translation="MLKSGKYIAEHDNGIFDRYRIVMSVKETEKSYIFELLEYVSRYS
                     SAQMDMLFDKSKRVLISKFKGGHAMRIWSDHDFTLYPYQAGIPFHFERVSEGGSAEGS
                     GVYG"
     gene            72697..73407
                     /locus_tag="ACG808_000086"
     CDS             72697..73407
                     /locus_tag="ACG808_000086"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFS5045751.1"
                     /translation="MGKKKTYRGELVDEIRGLGQSQGLNHVFTTFLEISATCISAEMD
                     PSNAEEREKRYEEMTSAMDPKTLNGYAHMLALLALAIHEHEEDPCDILGSIYHELRLN
                     NEWNGQYFTPDNICRMMARLVNPVDERSETEAPIMINEPTCGSGTMVIGAVWAMKQKD
                     FDFRHKSFFVAQDIDIRCVWMAYIQLSLYGIPAVVIHGDTLAMKEWSRWYTPLASVPF
                     IKRECAVNSTEKGAATSG"
     gene            73400..73948
                     /locus_tag="ACG808_000087"
     CDS             73400..73948
                     /locus_tag="ACG808_000087"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045752.1"
                     /translation="MDSTEKSLDDLTFADLRVHYGTGRAFLIRQEYRRNVYGYRKGVK
                     TDLGDLEEKDWIQLATGLIQKSGEQQLQKNLLEWEQEHNYCNSSLKEMEVTALELHMA
                     RIFDDPLWVAYIPFNRKYRPEVLESARLVWVQTECCGIPGQITQEQLDQSAGNALGIT
                     CPICGRCSPFQVCTPKEVSGNG"
     gene            73941..74123
                     /locus_tag="ACG808_000088"
     CDS             73941..74123
                     /locus_tag="ACG808_000088"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045753.1"
                     /translation="MDKLFIIVKGGRVEEIYSTKTETELYAEILDFDSMERTEEMEKD
                     LQFRYEAIRELMYRID"
     gene            74137..76107
                     /gene="ligA"
                     /locus_tag="ACG808_000089"
     CDS             74137..76107
                     /gene="ligA"
                     /locus_tag="ACG808_000089"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFS5045754.1"
                     /translation="MGAKYKTNHERMLELIEQLTKADIAYYRDDNPIMTDRDYDILMD
                     ELKDIESRIGLVLSGSPTQKVSGEILESLAEVRHSKPMLSADKTKSVEDLVKFAAKQP
                     VLISWKMDGLTLVLRYENGQLVQAITRGREGTIGEDVTHTVRTFLNVPLTIPTKDSFE
                     VRGEGVISWANFEKINSGLEEPYTHPRNLASGSTRKLDAGEAKKRLLEFWAFELVSDY
                     LESESKLAQQQFLAHNGFSVVPYIYLDAVHDETMVRDAISAMDPKKFPYPVDGIIMEY
                     DDVAYGKGLGATGHHENRLIALKWEDELYETTFIGLDVAVTRTGMVSLTGIFEPVSID
                     GTQVSRAYLHNYENYLDLALGRGDKIRVYKANMIIPQIAENLNKTGTCCVPLNCPCCG
                     KMLSFRKSSGGIRQLFCENPHCSAKLVRKFVHFCEKTRMNIEGLSEKTLEQFVSHGWI
                     RTFADLYALDQHRDEIVQTEGFGEKSFERLQASIEKSRHCTLAKFIAGLGIPMVGRHA
                     GRDLDRHFHGSWEAFEQAIQDGFDFTQLPDFGETMHNNIYTWYADTEEEKLWRPLLEK
                     IEFEKENFDMNTNTANPFYGKTVVATGKLENYTRDGIQMKLMQLGAKPASSVTKKTDY
                     LIVGEKAGSKLTKAQQLGIRTLTEEEFEAMLA"
     gene            76292..76627
                     /locus_tag="ACG808_000090"
     CDS             76292..76627
                     /locus_tag="ACG808_000090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045755.1"
                     /translation="MSASRLRIILMRMTFGIVPIVASAQRDVSSEISLRSRKETKNMN
                     QQKKNLEITGRLICPLSVGTAAFIAENGGIRRTSNVLRMERISPDEIRFETCNTNYRL
                     HLIRQEVTA"
     gene            76624..77370
                     /locus_tag="ACG808_000091"
     CDS             76624..77370
                     /locus_tag="ACG808_000091"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045756.1"
                     /translation="MSSEIFYDKAFILVGEKYIPVVNHGSSNCFDFDSRGREIPEKHW
                     SVLNYPHTGRMLFTAEEMQEIAAVHEEANRNNRGGTRKSRNRSFEEGEFGRWILAGMK
                     SAHTVEDYRKHGNTVTVIDYDHDYWQRHCVSTTEELLDKIKELSGHSITVSFWDDRHV
                     THPPMRRKGTPFDFGTLPEFYVLRAAQGYFVKRSSRKIWFARFQKPKSQMIRKFKTEK
                     AAQDYLDSNQKFFSGYAFEIECVQNGGVTA"
     gene            77433..77861
                     /locus_tag="ACG808_000092"
     CDS             77433..77861
                     /locus_tag="ACG808_000092"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045757.1"
                     /translation="MVRLLGNCSNLAVYAFTQEQVQQIFATLRDELDRAQKRYTQSYK
                     KRFSLSDEQYASLPKYPTIVLPLPDGSSLRAVAIDDENFPAINVYWDTEKPEIDGPIC
                     FAEYNPERGPCHEVCIGAYQSDKEDTVYYKPYMAERESNE"
     gene            77854..78654
                     /locus_tag="ACG808_000093"
     CDS             77854..78654
                     /locus_tag="ACG808_000093"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045758.1"
                     /translation="MSNYIYCRTLKLDWKEVSRLIAECAGKILNRTIHGTAGYEDDHY
                     WGFQVTTDRFTIAEIDKLIRFVNGDEEMQQEAIPQDSDKSAAIGESLSRALLEKALRL
                     SWCHESTTESTLWLVNIREKRPAVYKRIVEISPHDICLDNLRSKSELIAYLHENGPTH
                     STLMDFCADYRERYHNELCWNYPISDGLHLGTFFVLVKEGVLALPYDDADKVDYELLC
                     LDDAKMCDRESMENLITEWDSFDRDLRSAMQGMRAFYRREEEQHESEN"
     gene            78638..79066
                     /locus_tag="ACG808_000094"
     CDS             78638..79066
                     /locus_tag="ACG808_000094"
                     /inference="COORDINATES: protein motif:HMM:NF025447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4262 domain-containing protein"
                     /protein_id="HFS5045759.1"
                     /translation="MNQKIDWLIHLVANGACDECGEVETDFLPYMCNAHTHGLERYGH
                     LDFQMVLFLPTEEIGRILNTLGLRVQSGERFRSGDMVSGIYEDCDVRLDEYDETGRKV
                     LRVIIPDANNIFPGEGDCMLPYCLQLLKTDELCVERGIPS"
     gene            79063..79440
                     /locus_tag="ACG808_000095"
     CDS             79063..79440
                     /locus_tag="ACG808_000095"
                     /inference="COORDINATES: protein motif:HMM:NF025554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YodL domain-containing protein"
                     /protein_id="HFS5045760.1"
                     /translation="MKITLYQIIPEPENRYLIFSDLRSIRAASGGHVPAEIYEAVFSG
                     DLDIAVPRNAKNKMDQELAELEAVYVRFNVSHPEGFRGRSMTMSDVVEVIRSEKESRF
                     FFCDRFGFEEIAFEKEKADFGRL"
     gene            79461..79673
                     /locus_tag="ACG808_000096"
     CDS             79461..79673
                     /locus_tag="ACG808_000096"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045761.1"
                     /translation="MKKLILNYKGRDSWDRPVYESEGRLYVDVDPRKGWKPNICTKYN
                     NEFDGEPDTPIAEDTVVEFVPCRDIW"
     gene            79687..80367
                     /locus_tag="ACG808_000097"
     CDS             79687..80367
                     /locus_tag="ACG808_000097"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFS5045762.1"
                     /translation="MKEFMTKMVPVNLSDRFPFKCRMCGACCRHVRESVPLESLDAFR
                     LAKHLRDKGENVSCVEDVLAAYAEPVLLSENGYTVFMLKTVGADEACIFLKDNRCTIH
                     SVHPRACRTYPIAVGPYELGGYEQYLSMEQPHHYSGPQMSVKKWIQKRCSKQDFEFLN
                     TDIGSAQEIEKLLKKIPEHNRTRAVMMFLFYKYSDYDLDKSFLPQFKSNNDKLLEVLR
                     KMADDKDN"
     gene            80441..81172
                     /locus_tag="ACG808_000098"
     CDS             80441..81172
                     /locus_tag="ACG808_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFS5045763.1"
                     /translation="MDERLKNIVDNFEAMKIGVDEPFPFHCTMCGKCCINREDILLTP
                     RDIYNMAKELGITTKELFETYCETYIGCDSRVPIVRLKPRGSIKRCPLMKDRKCSVHK
                     AKPAVCALFPIGRCLMFEKDSNPSEKISASQIQYIFTNPGCGDNAETHTVREWLGEFG
                     MSLEDEFFVKWQQAIMEFGQFFREAEKTASERIMELSWTAAFVGLYLHYETDQEFLPQ
                     FEKNVREITALLQMAPIKEGGRANE"
     gene            81222..81830
                     /locus_tag="ACG808_000099"
     CDS             81222..81830
                     /locus_tag="ACG808_000099"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045764.1"
                     /translation="MESLNDAFEIGRIHLAFATYDLSRPIGQRQTNNIHIYIAVDEFL
                     ELCRKLEGGELRYLLKNKKTSGDKTPLYQCLGGTSAEKLAKYGRSRADGKSLSRTAQL
                     LAGSKSDFLFVADSGPGETDQKGLIVPKFGSKPENHVAVSMTFETFSELLLTTRLHYS
                     AWLGAWYANQYHPGNQRTAQAQENPQYQEKQGEAEYASDPMF"
     gene            82072..82821
                     /locus_tag="ACG808_000100"
     CDS             82072..82821
                     /locus_tag="ACG808_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS5045765.1"
                     /translation="MNTYTTSEVAKMIGIHPNTVRLYEEWGLIPNAERKANGYRIFTD
                     FHIEQFRLARTAFQIEVLQNGLRKKIVETVKVSAKKDFDQALVLANEYLSQIQKEERN
                     AEEAIAITMQILEGKAISETPPRCLKRKEVSEYLNISMDTLRNWELNGLMRIKRKQNG
                     YRYYTDEDIKRLKIIRSLRCANYSLEAILRMLHALSDDPHTNIKRVLNTPKDDTDIIS
                     VCDRLIISLQKAAENAKKMIGILNDMKSIFS"
     gene            82895..83629
                     /locus_tag="ACG808_000101"
     CDS             82895..83629
                     /locus_tag="ACG808_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5045766.1"
                     /translation="MEEIISVKQLSKSYGRLNAVQGLDLSVTQGSVFGLLGANGAGKS
                     TTIECMLGTRKPDAGTVSILGMAPLLQRKQLFENVGVQFQEANYQDKVTVSELCEVTQ
                     SLYKNAADYGELLKQFGIFDKAKSMVKELSGGQRQKLFIVLALIPQPKVVFLDELTTG
                     LDAKARREVWKILSDLKEKGLTIFLTSHFMDEVEALCDTICILKKGKAVFYGTVEEAI
                     AGSPYDKFEDAYLWYSDEGVSENENI"
     gene            83616..84359
                     /locus_tag="ACG808_000102"
     CDS             83616..84359
                     /locus_tag="ACG808_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5045767.1"
                     /translation="MRTFKTMLKNEMKLSLRDMNMVIFAICIPLVVLIILGVIYGDKP
                     AFEGAEYTFLDQSFGALSTIAICAGGVMGLPLVVSDYRSRKILKRFKVTPVSPVMILT
                     VQVVIYIIYSVASLALLYIVSTLFFGFHLLGSLPAFLGGYILVMLSIFSIGMMVGGIS
                     PNSQTASVIASLLYFPMLIFSGATLPYEVMPSALQKIADILPLTQGIKILKAASLGLP
                     IENIFVPLIVMAVLAVICIGVSFRFFRWE"
     gene            84613..84945
                     /locus_tag="ACG808_000103"
                     /pseudo
     CDS             84613..84945
                     /locus_tag="ACG808_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576328.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
     gene            complement(85165..85512)
                     /locus_tag="ACG808_000104"
     CDS             complement(85165..85512)
                     /locus_tag="ACG808_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007705912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFS5045768.1"
                     /translation="MPKEREKESFASLGDDIKAARQVLNISRRELAKMVNIDPRYLAN
                     IENSGSLPVFYQLVKICKLPVERYFFPEAGEQDDPERQRTAHKLSLCPKQYLLVIEGT
                     IDGAIGIEEAEDA"
     gene            85709..86149
                     /locus_tag="ACG808_000105"
     CDS             85709..86149
                     /locus_tag="ACG808_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008689490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045769.1"
                     /translation="MLKLYFGNSITIISTLLLGANIAYMLWGYLGRQTIQKWGMILLL
                     FILLHGAFWYFANVRDLYSNSIIFATDGSVEMGLFSVSSIQSIVFWAASVIVWLLGIV
                     SIFKLEYRQHIFYIIAIVSLVQIAFIEGSRIWLYCSAPAHFDYL"
     gene            86163..86795
                     /locus_tag="ACG808_000106"
     CDS             86163..86795
                     /locus_tag="ACG808_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008689492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5045770.1"
                     /translation="MAKRNTKDIILFESLKLFADKGYDGVTVRDIAAEVGIKQSSLYK
                     HYKSKQEIFDTLVDTMQSRFRDASTSFQLPNGELREMAKEYATGGNEILKKISSEIFH
                     FYLKDPYASQFRKMLSIEKYKNQEIDRIYREVYIDTAISYQAALFEEMINQGFMRQAD
                     PEIMALQFFAPIFLLLNKYDGIVEKEKEAIAILGKHIEQFDMIYRKEAIL"
     gene            86792..87280
                     /locus_tag="ACG808_000107"
     CDS             86792..87280
                     /locus_tag="ACG808_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008689495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HFS5045771.1"
                     /translation="MKAAIVYATKYGCTKKCAEMLKTYLNGEADILSAKSDKISLAQY
                     DTVFIGGSVYMGKIQKEITHFCKRNLKHLLHKKIGLFACCYTPKDTEGFFETLFPLEL
                     LHHASYVTSVGGKMDYDKMNFLYRKLFQSLKKIDGFNEGFIEPEINADEIKKLAEVAN
                     MN"
     gene            87311..87520
                     /locus_tag="ACG808_000108"
     CDS             87311..87520
                     /locus_tag="ACG808_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFS5045772.1"
                     /translation="MCPICGNKTRLKIREDTGLINFPLYCPKCKQETLICIKQLHISV
                     IKEPDAKTQSRQPMSLILMVIGFFF"
     gene            87867..88259
                     /locus_tag="ACG808_000109"
     CDS             87867..88259
                     /locus_tag="ACG808_000109"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5045773.1"
                     /translation="MAKDNGAPQSANWISSGFGKLLAGLDMPHIRYHDLRHTAATNMH
                     QLTGDFYTVGEVLGHTLAGIGASLGLSMNFEAVTTRYVDVRLERKQEVLEAYHAAVHP
                     TLKQAGAKEPGPDRAKPAQKKKSHEMER"
     gene            complement(88756..89148)
                     /locus_tag="ACG808_000110"
     CDS             complement(88756..89148)
                     /locus_tag="ACG808_000110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045774.1"
                     /translation="MKNKVYIGYIIDKSQKDKTIFNNLTVTNKKSIWLGLITIYEIQV
                     NEEKINTVINCLQKNMVDQIGFVKQEFYFHFYCENELIVVYRNKIFHATADKSTWEPA
                     YNYGKSLGIKEKQLDFLTPSENKERYFS"
     gene            complement(89175..89792)
                     /locus_tag="ACG808_000111"
     CDS             complement(89175..89792)
                     /locus_tag="ACG808_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5045775.1"
                     /translation="MKLSEKIQILRKRDGYSQEELAEICNVSRQSISKWEADIALPET
                     EKLIILSNTFKVTVDVLLKDTLSVEGIKEVSSCGNNAIKKKKSALFEGALIKESIEDE
                     NILDYIYVHKVELWNTGGKPKYWTVIFFTSAQPDFPELVSKVMISDKNRGGNWFVDFK
                     QENTKFIVFRNKILKYEIGNAKEKSIVCDECRKLGIPDEQMHWQE"
     gene            complement(90488..91066)
                     /locus_tag="ACG808_000112"
     CDS             complement(90488..91066)
                     /locus_tag="ACG808_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008689488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFS5045776.1"
                     /translation="MKLQDYIQNKPILDTERLLLRPLQYDDIDDLKEWLGDKSLYQYW
                     GKRPGKSDLNPELLFQKKERPTKSFHWGIVYKKDNKVIGEMWVYLIENDRMAKVAFRL
                     SPIYQGNGLMAEALTEVVIFCFEKTELQRLWADVHVLNIASYKTLEKAGFKREGHIRD
                     GKMVNTYCDYYLYGMTRTDYYETKGMTQLQSK"
     gene            complement(91076..91552)
                     /locus_tag="ACG808_000113"
     CDS             complement(91076..91552)
                     /locus_tag="ACG808_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HFS5045777.1"
                     /translation="MNDYQLKPIGKVKNDHNGAFIELEPEYIPGLQALEGFSHINVIW
                     WFSDCDNQADRKLLQTEQPYKNAPAVMGVFATRSPARPNPIALTTSEIISIDLNRGII
                     RLTFIDANDNTPVLDIKPYTPSFDRVETPGVPVWCSEWPRSTEASGCFNWEQVFNF"
     gene            complement(91654..92466)
                     /locus_tag="ACG808_000114"
     CDS             complement(91654..92466)
                     /locus_tag="ACG808_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS5045778.1"
                     /translation="MFRIGEFSKLTQVSIRMLRYYDEVGLLKPAEVDKWTGHRMYSVT
                     QIPSLNRILYLRDSGFLVSEIALALEMNDRLLLETLEKKRLEIEQTIQNEQEKIRKIA
                     IAKSEIQENKGELHYNISIKAIPEYQVLSLRRVVPTYYSEGDLWNELSAFAKEQKIEM
                     SNHTFSVYHDTEYKEQNVDIELCVPVNKLCKDIAPFCFRTIEPVPVMACTMVYGDFSN
                     IKGAYIAFAEWLQKNSEYRMSNPMRQIVHRGAWNECNPAKYLTEIQIPLELR"
     gene            complement(92574..92903)
                     /locus_tag="ACG808_000115"
     CDS             complement(92574..92903)
                     /locus_tag="ACG808_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFS5045779.1"
                     /translation="MANRTRNFVLRVPVTPEERALIQQKMAQLHTKNFSAYARKMLID
                     GYIVHMDTRDIRAQTAELQKIGANVNQIARRINSTGIVYAQDIEDIKGALAKIWQLQR
                     YILSSQR"
     gene            93229..93441
                     /locus_tag="ACG808_000116"
     CDS             93229..93441
                     /locus_tag="ACG808_000116"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045780.1"
                     /translation="MADEKLNTGPGEQNTPPAPGPVTEEQPQAPAPEQAAAPQPEQSG
                     PAQPEAGDVVVSFDKINELMAEKRRP"
     gene            93886..94719
                     /locus_tag="ACG808_000117"
     CDS             93886..94719
                     /locus_tag="ACG808_000117"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014124402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5045781.1"
                     /translation="MYDNLLTIFEKPSLYKKMEIPFWDDEHISLQMLKAHLNPEFNGA
                     SRKLDFINKSADWITRIVRPSEFNELLDIGCGPGIYAERFAQFGYSVTGIDFSKRSIN
                     YATSSAAKQNLNIQYLYQDYLNMNLNKTFDFSTLIYCDYGALSSEDRKTLLKVVYTHL
                     RPGGKFLLDVFSMSKYNCFEEKNTWNIYPTGGFWKKEKYLEFNSSFRYSDCVTLEQTS
                     IISPNDPAAIYYIWNTYFTKETLINEMKSEGFKICEVFSDVAGKPYCEDSLTIAVLLE
                     K"
     gene            complement(94790..95095)
                     /locus_tag="ACG808_000118"
                     /pseudo
     CDS             complement(94790..95095)
                     /locus_tag="ACG808_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860255.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            95555..95749
                     /locus_tag="ACG808_000119"
     CDS             95555..95749
                     /locus_tag="ACG808_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005930852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFS5045782.1"
                     /translation="MDYMTLKEAGEKWGVTPRWINYYCAASRIPGAIKMGTVWLIPKD
                     AEKPLDMRTKQGKELKHETI"
     gene            95736..96407
                     /locus_tag="ACG808_000120"
     CDS             95736..96407
                     /locus_tag="ACG808_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5045783.1"
                     /translation="MKPYKILIIEDDPVIQSELEILLRGNGYTAVSVKDFSKVNDTFR
                     AEDPHLVLLDIKLPNESGFSICSQIRSNSNVPIIFVTSCNTDMDELNSIMLGGDAFIT
                     KPYHTAILLAKIASLLKRSYPVQQNEQMVYGATVLHLESSSLDYDGQSVELTKNELKI
                     LYYLFKNAGKICARRDIVEFLWDNQLYVDDNALSVNINRIREKLTGIGLTDFIKTKHR
                     QGYTV"
     gene            96404..97417
                     /locus_tag="ACG808_000121"
     CDS             96404..97417
                     /locus_tag="ACG808_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002578902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS5045784.1"
                     /translation="MNRRQYWKNRIPFLLTNLICMVALTVFLLVCGNPISVVLLISAV
                     WALILLAGLFLTYWKRQRQMRQLLDMTGQLSERYLVSEVMELPEQAEDQVYYRLLKMA
                     GKSMLEQIGTVCQERQEYKEYIEQWIHEIKTPITAMKLLCENHRTDWTKELLLELEKT
                     NRFTEQALYYARSEHTEKDYSVREMALAQVVHQAIADNKYLLLQSGVRLKVEEMQDTV
                     YSDEKWVRFILNQLIANAVKYRAEQPIIRFSARRQRDQVVLVVEDNGIGISLADLPRV
                     FEKGFTGQNGRMIQQSTGIGLYLCKRLCDKLGISITAASSEHGASISLAFHINCLIHE
                     VQS"
     gene            97519..98271
                     /locus_tag="ACG808_000122"
     CDS             97519..98271
                     /locus_tag="ACG808_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5045785.1"
                     /translation="MKEVLKLEHIQKYYGNGGNITKAIQDISFSVQEGEFVGIMGASG
                     SGKTTLLNCISTIDTVSAGHIHLDGTDVTEINEKQIARFRRENLGFVFQDFNLLDTLT
                     ISENIALALTINKVPAGEIDGRVQEMSSKLNITDILKKYPYQVSGGQKQRCACARAII
                     NNPKLILADEPTGALDSHSSQMLLSTIQSINETLGATILMVTHDAFSASYANRILFLR
                     DGTIFTEIRKGNDSRRTFFNKILDVLTMMGGN"
     gene            98276..100330
                     /locus_tag="ACG808_000123"
     CDS             98276..100330
                     /locus_tag="ACG808_000123"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5045786.1"
                     /translation="MLASLSQRNMKRQLSEYKIFWFSLIGVVALMYAFNSLIVSHTMQ
                     QLFRLFAESGNGDIGVIAALFSAVVVFALGWFISYMMDFILQRRSKEISTYMILGVEK
                     GDICKMIFKENAFLGLMAVIVGFGFGILVSKILESFVSNLFHFQEALLAFLSVSAVGL
                     TCIEFCIVYIIALIKTNRKVQTVKLIDLLNYSRNNSSVREHQSKTGFMFLLISVVMLI
                     VSFYLFAGTKQTVASITAGAVSAMLGLLFFFRGFIYIIHEMLNKSQNWKYKGSRLVTL
                     RMFLSKSNKMSFSLGVISILFTCTIVCIGMTNAFYQVMEKAVVMQPFDLAIVHVGENG
                     DYSQYSSFLNERIDVDNQYSYCLYTDKSTQFTDIRNRVLTKYWNRASKTVSINDYVIA
                     ENQYDAFMKYSDYCNLRAMLGLPQIPLSEEQYIIHCLPYLKDTFTNFQIEKGTLVCKD
                     IFTEAFSQYGGYGNGQDFVIVVPDHYIENMEAMYSLYVAQSETVIDMSELENEFPQVR
                     PLNSNVVASGENGYTTKILDKGNYYYMGKLASTPTSQAILIILPLCYLSLVIGIISIV
                     ILAVQLLTEVKTIKRQYNVMRTLGNEVIVLEKMLRDHIFLYFALPFIPAIVIGSCLLK
                     SMSHTLFIASYDVPVFDNLTALIALVVLSALLIFTLIYLLYAFIASQSMRKEIIPLTL
                     EK"
     gene            complement(100601..100816)
                     /locus_tag="ACG808_000124"
     CDS             complement(100601..100816)
                     /locus_tag="ACG808_000124"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5045787.1"
                     /translation="MGRALQKPPITQEELAQRIQFMGMEDMTPLIISRIEKNQRHVCD
                     AELLMLAKALHVSMEWLVSDTDEIKIK"
     gene            101133..101615
                     /locus_tag="ACG808_000125"
     CDS             101133..101615
                     /locus_tag="ACG808_000125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFS5045788.1"
                     /translation="MEKKICYTYIVYKWVLRNLLVFLFSGKEFDTMKYCPYCGKELAN
                     PAASFCTECGESLSSAVPEEAPVTEAQREKKKSSKKHVKKKRYAGKTKRPDVPAKAGG
                     ASEPVREDDYDGYYDDVRPIDEGGGREEIDKALVKKIILVVACVLLIAGACVALMYLI
                     "
     gene            101690..102253
                     /locus_tag="ACG808_000126"
     CDS             101690..102253
                     /locus_tag="ACG808_000126"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045789.1"
                     /translation="MIYLSKGIVKENSTEHLLQVARCGQEYSLSGEQAVLWLNGRFGF
                     SEVKTESEKRTLKHLARMGLAETGAENTDVARYRILTQCVCCPAINAKPEIFLSRAEK
                     EILMWLWNAGLRLTVAEIIFLREHKIRPEPRYLHAENRQALVEAIYTKNTIADNCLEQ
                     IMECAECRDETIRILLGLLKKKKLIVL"
     gene            102255..102713
                     /locus_tag="ACG808_000127"
     CDS             102255..102713
                     /locus_tag="ACG808_000127"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045790.1"
                     /translation="MKQMKERFYELPAPLQKQIAIRIVGGAVFLLLFVIILLGFRDFY
                     FSLPCLLLSGFLLINGIRLCYNSFAGNYMCIQGTCEKIETVGLRKRTKGIWVQLEGNV
                     VKIPIRQRMKVLSIGDTVIIYLSDKTPVYEHEGNYMICSYYALGIEKRGN"
     gene            102715..102876
                     /locus_tag="ACG808_000128"
     CDS             102715..102876
                     /locus_tag="ACG808_000128"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045791.1"
                     /translation="MEADKEMLNRLLNEIQELVKENENEDGTYRFDLLKAVIALDFAK
                     TEIEKAIFK"
     gene            103097..103594
                     /locus_tag="ACG808_000129"
     CDS             103097..103594
                     /locus_tag="ACG808_000129"
                     /inference="COORDINATES: protein motif:HMM:NF012651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFS5045792.1"
                     /translation="MLKVFATQVVVSKGYDNTPALRFSENGDSVRFRIGKKVYDPHAE
                     NDSRWINLPVKAFNGVCERIKKMQLKEGSFVNLIGRLDEDSWEDKDTKETRKMMVIIL
                     DDIEYASGGGKSKENQAAAGQQNAAPQNTAPQNAASVPATGAEASGNFTGYEAFGGGS
                     FFDEN"
     gene            103691..104071
                     /locus_tag="ACG808_000130"
     CDS             103691..104071
                     /locus_tag="ACG808_000130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045793.1"
                     /translation="MTNETFCLLSEEEKHTKFKAAMKGKTKGCLNEEELHSILMSISL
                     NRQYAISVYRDGEEFCRVLTATNGLKRRTILGHAAADIGEIPGKQFDSSQAAAVILHD
                     AVDCSDVNCLYIFIPKERSQKGTE"
     gene            104071..104826
                     /locus_tag="ACG808_000131"
     CDS             104071..104826
                     /locus_tag="ACG808_000131"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045794.1"
                     /translation="MNKKDQKRHMAYEALVLLGMLALLTFICRLWPILLLIILGIFAA
                     VIRLLFLSSRKIEVIVPLPLLPEPVKETTEKDVQVLAYSVILRRVTELVLSEYPEARW
                     VWEAPNAEKLIQESQEVFILLNRAGGYRRAKVVIRNLQVVGIEYNPQVEENAVLEPED
                     NAPEPEEEPEVQNYELLAFEWADAHIFELNARCNEAIGENLSELILLAEELPVRESWA
                     DICRELIRAGLTDVQCVSEGIKINLTQSNAERK"
     gene            104830..106704
                     /locus_tag="ACG808_000132"
     CDS             104830..106704
                     /locus_tag="ACG808_000132"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5045795.1"
                     /translation="MSAYMNNIFNYSRPLPEPFDTLTNKKVSVSSKYGDGTNATLCST
                     VIKAVHAVCRCMDGSAEGAVGVIDHRTVAEYKSSMGPDEYHLVVYDSNSGSLMASVYD
                     KNTETFENYVLNASGRDGAAVMMALFPVLMNDEEFSDNFELYRDQFSHGFSDLRSATE
                     YMAMLCDNAYRRIKDTSCSAAVKVSVDKAGNLMRVSQVQLDSGAFEPTHVIAGEFTIF
                     AKTARVIVKSADAIVEHTDFVGKYELHPRTMSSQEKQLIPVLPEWYIIPQEVVDICKH
                     AQATTGKPTQMRNFLLRGPAGTGKTMSAKAIAAGLGLPYMAYTCSAGTEIFDFIGQIF
                     PDSDSGSTGDAQLDHEKAILASMGGINYANVSKMMNLPDLDDMDYDPAGVYQALTGVE
                     NAAATSQDCMSIVLDRVTEKVRALSRRDENSKGGGQTYTYTETDFIKALKNGYVIEIQ
                     EPSTIVQPGVLVGLNSLLEQTGTITLPTGEIIKRHPDAVVVVTTNIEYEGCRGMNQSV
                     IDRMSLVRDVELPTPEVMVQRAMSVTGATDEYQISQMVQVVNDLSEYCRKNSITDGSY
                     GMRSLIDWIVSSEITGDVYESALYTIISKATTDEVDREALVSAVLEPIFTKKRKKATA
                     "
     gene            106732..109002
                     /locus_tag="ACG808_000133"
     CDS             106732..109002
                     /locus_tag="ACG808_000133"
                     /inference="COORDINATES: protein motif:HMM:NF023203.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045796.1"
                     /translation="MARVNHKLVKQRLNEQRSKISDRQFFSSRLLAGHFEDLAAAQTR
                     RYRYNRRVRVNLYWKPREKHVASTDNMFVRINTGNSLVTKVKGRENRYQIVCGLFAHE
                     LGHVLYTDFLAAQTHTNYLGSGRWYPYPPDLKTTADARNEKAFWNYVKTDPKNLEMVQ
                     MIAHHISNVIEDGYIENRMLNNFPGTLGYGLEKLREQHFEHIETVTQMIESEDEGKHI
                     FESILQIMLSYAKFGEIKYGDEPLSDERIQAVFGLITDIDSALLSRSGKDRLNVVNMV
                     LVRCWDYIESFCEECKKRQEEAVASGGSASLAETLSEVLGAIAGGSEMGEGSSTPVPE
                     ASGGSEESATAGKRAQTHADADSEDDSEETDSSQTDSETEENPSESGGSDNSTDEDAA
                     PIDGGSSGSGKQETSDTEQGRIPYHQSESLSEPVGGSVEKNEDYEREHYDSAAADIER
                     LLDKMAEKAACEQLENERIQELNDVAQNISYGNIHEGVPIRINRIASVDEELMEQYDA
                     ISGPLLNISRQLQKSLLKQLKENRRGGKQTGLIMGRRLDAHALCRNDGKVFYKNNLPN
                     EIPELAVGLLLDESGSMCSCDRCTYARASAIILYDFCQSLDIPVMVYGHSTDYTDVGN
                     TVALYSYAEFDGFDHDDKYRMMDIAARGSNRDGAALRFVAEQLSKRPEAVKILILVSD
                     GQPADSGYGGSAAEEDLRGIKQEYQRKGILFVAAAIGDDKQNIERIYGDSFLDISDLN
                     QLPIKLTAVVKRHVRV"
     gene            109082..110206
                     /locus_tag="ACG808_000134"
     CDS             109082..110206
                     /locus_tag="ACG808_000134"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045797.1"
                     /translation="MHFLTLAALEISQIQEDKELDNQIAEALKELELQKQIETKNFML
                     DFAIGRCQNLQSSFSRAVNDGVSELMYPYCESLEDPEYLEFEDRTEKLREEYEDAVDC
                     IKLPQGTVVEQYGDPLWGRFVVRDGKVFQRDAGSLHHEKRTKKAKRMVALPNYPRKKL
                     YKSFEKYAEERCGFSFDEKHQGYGYYYNPNAIWDWYSIGGRWPEMFLVKDDCTEYSIG
                     ERSWCNSDRKSEAPEGYRWVCAARKKDIAWDAMRDWRNQKAAERFHKLEQMFLAGKTD
                     PDFHGEIVPDGVMHWGELVYRKGSTLEEYLEEYGIPGSWKYPVGSHDIVDEDQWLSKD
                     DSVLDAESEKYVPVDWRSCIDGYIDDVDEDTVLVAVDYHM"
     gene            110239..110475
                     /locus_tag="ACG808_000135"
     CDS             110239..110475
                     /locus_tag="ACG808_000135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045798.1"
                     /translation="MDSVWVRDSEMFDIVGLTDEELTDIDMCGTDNEGRWHDMEPTPF
                     IAVIKAESEEEACKKAATQMRYDPRCLFAIKVSE"
     gene            110491..111354
                     /locus_tag="ACG808_000136"
     CDS             110491..111354
                     /locus_tag="ACG808_000136"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045799.1"
                     /translation="MIINRIGAEFEYDGTTYVIGAPIVGTPESEYEGLYGTITEIRDG
                     EDKETENETPDIYCSFEVPALPCEVKKLEEVFSELYDQKKTIDDIILDLVIMAPSMVE
                     PLDDLKECRQHPRIYILLEDWAVDGEQGNSSEVYTDFNDAKRILVQKLKEEQESGCIP
                     QWVDDEKFKEHSTDSLYECYIDGEYCESHYHIAIVSQQFCVSNRFVREMGWLYQASCQ
                     LEDFVSQVSDWDELDQLTDEQYNRMVQDPRFPERLQNKLGKNDSYWESYWESVSEVAH
                     EFVSEYLKKET"
     gene            111360..111701
                     /locus_tag="ACG808_000137"
     CDS             111360..111701
                     /locus_tag="ACG808_000137"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045800.1"
                     /translation="MNTRINYLYRDADNYKMPNSCVVIGEISEVQIAEVISCLDCGEY
                     FIPRQVGLPEKRFDRFDEEVDHCWFELSADGFEATENVSNVDMTVVQLLELFRSAKNN
                     WHDDVPLGGAV"
     gene            111698..112135
                     /locus_tag="ACG808_000138"
     CDS             111698..112135
                     /locus_tag="ACG808_000138"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045801.1"
                     /translation="MSYSEWHTYGYGICVSDITDESVERLQKLISLAPEYQKKIQAWL
                     DECEISEPAYEDYLEFDQDYMLGLATILKEVILEAEDIDLVACDSHDGTDYLLYVPDY
                     PWNMGKHRQLMTEEAVAGLFRKYVSILTDEAIEIDYQSVENGG"
     gene            112147..113079
                     /locus_tag="ACG808_000139"
     CDS             112147..113079
                     /locus_tag="ACG808_000139"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045802.1"
                     /translation="MPYKSDTRPLTSIVSYPERGEGGNNRYRGNCSPRLIEDLLEFFQ
                     PAEICDYMCGSGTTKAAADKLGIGSHLYDLHSGFDIMNCEIPERPEFIFWHPPYWDIV
                     TYSDVMYKAADVQKSYGYDPRQYDLSRIPDWAGFVEKMNYAMMKQFSALEKGGRMAVL
                     MGDIKKKGVLYSMLSEIVKPGSLENIIIKAQHNCFSDQIQYSGKFIPILHEYVMIVRK
                     DSPLLVPIIMAKKTEVDIRDMPGATWRDVVAAVLEQCSEPVTLTFLYEKIEPHKKAQA
                     NKWWKEKIRQTLQINPTYFFHDGRGMWSLNRSAA"
     gene            113195..113521
                     /locus_tag="ACG808_000140"
     CDS             113195..113521
                     /locus_tag="ACG808_000140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045803.1"
                     /translation="MSVYYNPDTYRKIIRNGEECWVYDWGKNADINSFNYMLDRWEGT
                     QQQFFINGETKQYFLINKQERTVMEISNPEVIRFLYENLYMANINETFIELFTSIGGF
                     KTVKYE"
     gene            113660..114280
                     /locus_tag="ACG808_000141"
     CDS             113660..114280
                     /locus_tag="ACG808_000141"
                     /inference="COORDINATES: protein motif:HMM:NF024734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFS5045804.1"
                     /translation="MEPRRCIDTDTIWTKIQTIAEKSNGFVKTSDIEAAGISRPMLKK
                     YVDMGKLDPVRKGLYTLTDEFTDEFALLQAQSAKIIYSYGTALFFWGMSDRAPNILDI
                     TLPRGTNISRLRRDNPNLRCHYVQTEVYDLGITETKSPQGGMVKLYDRERCICDVIRD
                     KDQIELQLFTQAIKDYFKTNPNNRKLLKYGKVFGIEDKIRTYMEVL"
     gene            114280..115119
                     /locus_tag="ACG808_000142"
     CDS             114280..115119
                     /locus_tag="ACG808_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFS5045805.1"
                     /translation="MKTPEQLKGAIRSMAAKKNLRAQEVLQMFLFERIIDRLAASSFR
                     DNFILKGGLLISSMIGIGERTTMDMDTTARGIQMEEDEIVSAVKEIIAMDVGDGISFE
                     FQKIEPIREDDAYNNFRVHLRAKYGKIDSPMKIDITTGDIITPAAIRYDFPFVFEEKT
                     VSVMAYTLETVLAEKYETIIRRNIGTTRARDYYDLHTLYRSRKDVVQMEVLRAAVIHT
                     AEKRDSVDDIMDWRDILKDIREEPQLYLLWDNYAADNKYIGDLKFNEVLDTVDEIAKI
                     LDL"
     gene            115197..116180
                     /locus_tag="ACG808_000143"
     CDS             115197..116180
                     /locus_tag="ACG808_000143"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFS5045806.1"
                     /translation="MNKEELHRVIGEQQPNICQVVAVKDSNIIYSDTWNDFKQDDNVH
                     IASVTKSIIAILIGIAIDKGMIKSIHQKVLDFFPDYVIKRGEKTIHDITLYHLLTMTA
                     PYKFKSEPWTKVCMSEDWTKAVLDLLGGKSDIIGEFKYSTLGIHILSTIITKVSNMPT
                     LEFANKYLFKPLGIKNRHLYTVKNKEEHIGFVTSKEPKENGWFCDNKGTVTSGFGLCL
                     SAEDMAKIGQMCLDKGMYDNKRIVSSEWIEKITTPYLTTDEKFGFMQYGFLWWIIDSE
                     KKIYSAIGDSGNVIYVNTEKNIVISVTATFKPLVFDRVQFIREYIEPFVLG"
     gene            116665..117807
                     /locus_tag="ACG808_000144"
     CDS             116665..117807
                     /locus_tag="ACG808_000144"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045807.1"
                     /translation="MQKQCQDGFYTTFSSYPFMLDYHKEQSKNSRWVKARVSDLEIQP
                     LGKGSALSTDLPAFASGTTQDAVDDTANNLGLAMRVDGELFPMRMTAYKSLLDRAKIG
                     GTALPKLSREVLAEVLNACLRLYSADALLLIRDEKVAAVHSGDAVDYSVLPIDELLTA
                     LKTKLDARFSGNEFESGYCDHAMVSAAWTMPDQKEDLLGAYTKLLDSQGKTAMASKLT
                     PGVRFMSSDTGVASAKVSALLVSGKRSIHIGGCIAVDHRHQSKVSDFDTALDQLFAQF
                     GDSIAKLQKLLEIHLDYPVNAMTRVCKKLSLPKKAAVEAIAMYEMAYGGGPATAHDVF
                     LAMQEIPFILRTENTPESKMLVIEENMARALSFRWSDYDLAKAVSY"
     gene            117807..118844
                     /locus_tag="ACG808_000145"
     CDS             117807..118844
                     /locus_tag="ACG808_000145"
                     /inference="COORDINATES: protein motif:HMM:TIGR03033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda-exonuclease family protein"
                     /protein_id="HFS5045808.1"
                     /translation="MAKPIVLCDTAGMTNDRWLECRMHGPKGDIPYTVGGSDVAAIFG
                     VSPWTTPLELWKIKKGQMKPAKKANANQLQMGHLLEPVAAYWYGAKTGNTVTEDTNLY
                     QHADHPYALANFDRRFTRASDNEPGILECKSCTYHKADEWADGAIPLYYELQLRFYLA
                     VADVNIGAFSAVWGNNPDNDLAMPEIIRDKAKEDMIFERLDEWIWSLENDKPPTMADV
                     APKLALESLARIYGASQAGLPTIEFSRKYESPLRRIAMLQGKISECNREIKTYEKEIE
                     AHSVRIAEVMKEHEHGVLETTSDKLLIDFVTKTTRRPDSKALKEKYPAIYTDVLKASE
                     SRKVKVSVQPI"
     gene            118858..119382
                     /locus_tag="ACG808_000146"
     CDS             118858..119382
                     /locus_tag="ACG808_000146"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045809.1"
                     /translation="MENIYYEGWEQELIYQFLPYDRCKKRAYICSPLSADTNEGIAQN
                     MQATRAYMFYAMKKMRMNASAPHAYLPMILCDNIPSDRALALQFGLELLKGSDILLIC
                     GNRISSGMRGEIAHAIRLKIPMIAFDEGVYLEVQKELTKRDCDKRKVRLDRENFLMGI
                     SAPLSYLENAEMFR"
     gene            119488..121659
                     /locus_tag="ACG808_000147"
     CDS             119488..121659
                     /locus_tag="ACG808_000147"
                     /inference="COORDINATES: protein motif:HMM:TIGR01448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFS5045810.1"
                     /translation="MLCEFDRLIYPQSITAVDASSYMIALYHPCEKIKDSTGNTVTQV
                     KAVGYCLPTSSNLRYDMLGHWSKNPKFGVQFEVESYNEVVIPTKEGIIAYLSSGQIKG
                     IGPKIAEKIYAVFGQQSLEVLDKEPERLLAIPGISEIKLKKIYDSYLVNRGARDVVAF
                     LSPHGITPNRAVRLYKEYGEKTMDIVKNHPYQLCDMAGIGFKTADHIAMSMGFDQLST
                     ERVDEGLLYTLADAEAKGHLCMEKHEFVKACLKILDTPALTSEMVANRAARLVFSGQL
                     VSYQGNVYRAKTVHVEEQLASAIHQQMKHRKMHSYGDLDAAIDAEEQKLKMKFAPEQR
                     EAVKMALTQGLSIITGGPGTGKTLIQRAILDIYQKNNPKSEICCCAPTGRAARRMEQA
                     TGVPASTVHKALGLMADEDGDYDGPEALTADLIVVDEISMLDVYLAGYLFDAVKYGAQ
                     MVLIGDADQLPSVGPGAVLSEMIASGCIPVVRLDKVFRQNAGSRIATNAKLIRHGNVG
                     LEYGDDFQFINSPRLSDSAKLIVDLYLRETEKYGVDNVALLTPYRQKTETGVNALNEH
                     LREKVNPPDAQKPEVVFGNRKFRCGDKVMQIKNHDDVNNGDIGYIRKIIRIGDDTTVH
                     VDFGDGRMKEYDSSGLDMLDLGYASTIHKSQGSEYQSVIINLQCAHSIMLTRPLIYTA
                     ITRGKERVTIVGEKRALCISIKRTDTEKRGTCLAKRLQGLA"
     gene            121674..122117
                     /locus_tag="ACG808_000148"
     CDS             121674..122117
                     /locus_tag="ACG808_000148"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045811.1"
                     /translation="MATILDQYLEKQNSIRSQIAENSLPPEDLLIMQELNYRICVLET
                     FQSFCKSAPITMDTKVMGYHFQMVDAYVRFTLNERRFGLKADAEGQKRRETALSSFER
                     VVQDGRKRFSSFKAGTQEQYKTSISQYVHTILPVWMQYRNTYINL"
     gene            122131..122889
                     /locus_tag="ACG808_000149"
     CDS             122131..122889
                     /locus_tag="ACG808_000149"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045812.1"
                     /translation="MSQNNSNEKAAMLAKIETIHQVDGFDPTPFAVDYTDLGTGEVRK
                     RLPVMIQMAWFRLKYPEGRIAVQVNSAKDCFVATARVYPNYKDAADCYLAEATASRGY
                     CEEKPSVSPREWAQTAAVGIALRNAGFGLQFSMAGEDFEPSAVDELGISAAQPQTESA
                     EKHEVSEPKPEPAQEEYTVNEPVQKELTPEEKLAQAMNVPCPISKFSGKTLGEVLSLD
                     PGAIKWVATKFTGGEEIKAAAKLICEFSLQQTIA"
     gene            122961..123338
                     /locus_tag="ACG808_000150"
     CDS             122961..123338
                     /locus_tag="ACG808_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4313 domain-containing protein"
                     /protein_id="HFS5045813.1"
                     /translation="MCKKTKKLRFKWEFGDEMVSLHVNQYAYGGRLHIGMINYGEDGP
                     EPFADMTVNLPAYDLEDNEAFINGDISKDLLRFIRENKLGKVMPYTVKSGYGRYAAVA
                     FDLEKLAEYDPTGVASFKENCGL"
     gene            123412..124470
                     /locus_tag="ACG808_000151"
     CDS             123412..124470
                     /locus_tag="ACG808_000151"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3854 domain-containing protein"
                     /protein_id="HFS5045814.1"
                     /translation="MEIFHMSDVVSLLGIPMPPSGRSSYYVQCPCCDENPRKKHLNIN
                     LKKEVYRCPRCGIYGGIFDLYSLYTGVPRDKVRKALVEKLGMPDHRPKPQKRIQPQAK
                     PECPITDVDTRHATYSALLDKLTLAADHKENLLGRGLTEDDIIRLGYRTTPVVGMTAI
                     ADKLQSEGMYLAGVPGFYRNESGTWTFIHEERGILIPMRDRQGRIQGLQIRRDKVKKR
                     KFRWVSSTERPDGCPAEGWTHLAGPVRPMLVLTEGPMKADVIHALTGLTVLAVPGVNS
                     LTQLETTLSDLRAEGVAEIKTAFDMDFATNYHVQNGYNNLLSLLGNMGFQFGTYLWDP
                     RYKGLDDYIWEYCFQKRH"
     gene            124604..125524
                     /locus_tag="ACG808_000152"
     CDS             124604..125524
                     /locus_tag="ACG808_000152"
                     /inference="COORDINATES: protein motif:HMM:NF037161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HFS5045815.1"
                     /translation="MLIAIDHGNYAIKTTNHSFISGLSEHTVKPPMTDEILEYGGKYW
                     TLSGRRLSYMRDKTQDDRYFILTLFAIAKELENGGNYTPVEQVQLAVGLPPEHFGALK
                     DRFANYFKRDGIIKFVYGDRPYSIKIDRVMVFPQAYAAVIPQSSLVVHTVRMFIVDIG
                     GYTTDVLLLKNGKPDLQFCRSLETGIITMNNEIIRKVGALHDMRIEDEHISAVLQCEN
                     TILPEDVKETIREATKLHAQDILNQLRELQVDLRSNPAVFIGGGSILLRPFLINSPLV
                     AKADFVESPNANALGYEMLGRKTLSLRPLG"
     gene            125544..125906
                     /locus_tag="ACG808_000153"
     CDS             125544..125906
                     /locus_tag="ACG808_000153"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045816.1"
                     /translation="MKKDGKYRFSLQFSADSEEQVRAGELLERLGNRKSAVVVAALND
                     YLDSNPNLQDAHCKIEVKLTPGYNRDGIEEIIRRIVEERLAAVRRDEPQMAFSEESKT
                     DTLEADIAQMLDNLDMFS"
     gene            126076..126420
                     /locus_tag="ACG808_000154"
     CDS             126076..126420
                     /locus_tag="ACG808_000154"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5045817.1"
                     /translation="MDYVILGKNIRKYRQMRGMRQKDLAEICDCGNSHIGQIENARGI
                     PSLDMIVRIANALSVTVDQLLKEYYSEPEKVYLREIAERIEKYPVKQRVYACEGLAEF
                     LNTIEKFSQTDE"
     gene            126644..127054
                     /locus_tag="ACG808_000155"
     CDS             126644..127054
                     /locus_tag="ACG808_000155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS5045818.1"
                     /translation="MNHQQKSERDELRARFTVWLETTVYRARLKYLEREKPKVDTVSI
                     EELPESALSVLEQTSHCGISGSAFDFEEERLAEAFAKLPIKRQEILTMLFVEERKPEE
                     IARLLNCSPQHVYDQRYQALKKLRIALTKDGDKF"
     gene            127051..127242
                     /locus_tag="ACG808_000156"
     CDS             127051..127242
                     /locus_tag="ACG808_000156"
                     /inference="COORDINATES: protein motif:HMM:NF024059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5045819.1"
                     /translation="MNRIEFEKMLQAAVSGSHEALEQLFLLYAPLIDKHSKIDGQIDE
                     DLRQYLLIHIALNISKFVI"
     gene            127621..127830
                     /locus_tag="ACG808_000157"
     CDS             127621..127830
                     /locus_tag="ACG808_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008976866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6462 family protein"
                     /protein_id="HFS5045820.1"
                     /translation="MGYKRKDVEQTKQYAKKFVRYKEGAEKYSLGLTKFQELAKEAKA
                     VYKIDGIALVNCEIFEKFLESFREF"
     gene            127938..128108
                     /locus_tag="ACG808_000158"
     CDS             127938..128108
                     /locus_tag="ACG808_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004606461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045821.1"
                     /translation="MTKMGRPKVDNAKRKSITVRLSDKTYDELTRYAAEHKMTKTEVA
                     LRSLEEYLSKQK"
     gene            128151..129383
                     /locus_tag="ACG808_000159"
     CDS             128151..129383
                     /locus_tag="ACG808_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5045822.1"
                     /translation="MAKTRKDERGRALRKGEVQRASDKRYMYTYTDPLGRRRSIYAQD
                     LATLREKEKQLMKDQLDGLDLYVAGKATINDTYDRYISTKYDLRATTRSNYDYMYNRF
                     VRHTFGKKKIADIKYSDVLQFYCYLLKKEKLSLGTLDSVHTLLHPTFQLAVRDEIIRK
                     NPSDGVMKEVSKKADKTRGVRHALTREQQRAFMEYISNHPVYYHWWPLFTVLLGTGCR
                     IGEALGLRWDDLDFDNRILSINHSLVYYPVGESRKSVLRISKPKTEAGIRTIPMLDIV
                     RDAFHMEHEEQEETGFNETEIDGMTGFVFVNRFGSVLNPQAVNRTIKRIISGYNAEEV
                     INAKRERREPIILPDFSCHHLRHTFCTRLCEHETNLKVIQAIMGHRNIETTMDIYAEA
                     TDQKKQESFENLSKLDIF"
BASE COUNT        38130 a        24998 c        32099 g        34556 t
ORIGIN      
        1 ctgatatgac tcccgaaaac cggaacaaaa accggttttc gggagatttc tttatttaag
       61 ttgtaaatag ggtgatgtcc tgttttctag atgattctga attattttta aaagaagcat
      121 tcccaagttg aaaaaagcag tagttgctag tatgattcac tatgagttta aaaacggaaa
      181 aaggagtaca taacaaatga aagattattt ggttaaggca cttgctcacg acggattcgt
      241 ccgagcatac gccgttcaag caacaaatac cgtagcggag gcacaaagac gtcatgatac
      301 ttggcacacc tcctcagctg ctttgggacg gacattggtc ggtagtctgc tacttggtgc
      361 gacgctcaaa ggagaagaca aactaactgt tcgaatccaa ggcgatggtc cagcaacagg
      421 aattgtagta gatgcaaatg gaaagggaga tgtgaaaggt tacatcaaaa atccgcatct
      481 cagtcttcct ctaaatagtg aaggtaaaat cgatgtgcgg ggagcagttg gaacacaagg
      541 aatgtttact gtcacaaaag acttaggatt aaaagaacct ttttctgggc aaacgccgat
      601 tgtttctgga gaaatcgctg aagattttac ttactatctt gctatctcag aacaaatccc
      661 ttcagctgtt ggactgagtg tattggtcaa tactgacgac agcatcaaga cggcgggagg
      721 attcatgatc caaatcatgc caggtgccga tgaagaaaca atcagcaaga tcgaagaaca
      781 attaaaaacg acaccaagaa tctctaccct cttagatgaa ggacagacac cagaagaaat
      841 attgaaagga ttgcttggta cagaagaatt gaacattttg gaaaaaatgc ctgttcaatt
      901 caaatgtgac tgttcaaaag aaaaatttgc agaagcaatt atcgcgttag gtgcagaaga
      961 aatccaagca atgatagacg aagatcacgg tgcagaagcg gtttgttctt tctgtaataa
     1021 caaatacaag tatagcgaag aagaattaga tggattgaag aaagaagcct taggagggat
     1081 ttaagtggaa cacacatgga aaattggcaa tgtggaaatc cctaatcgtg tcgttgttgc
     1141 accgatggct ggaataacaa attcagcatt tcgagtcact gtcaaagaat ttggtgcggg
     1201 cttagtcgta tgtgaaatga tcagtgacca aggtattcat ttcagaaaca aaaaaacttt
     1261 agagatgctc cacattgaag aaacagaaca tccattaagt ttacagatat ttggcggaaa
     1321 taaagatagt ctagttgaag ctgctcaatt tgtcgaagaa aacacagctg ccgatataat
     1381 agatatcaat atgggctgtc cagtaaataa agtaatcaaa gcagaagcag gagcaaaatg
     1441 gcttttagat cctgagaaag tatacgaaat ggtccaagct gtttcttcag cagtaaatat
     1501 cccagtaaca atcaagatgc gtattggttg ggatgaagag cacgtctttg ctgtagaaaa
     1561 tgcattagca gcacaatcag ctggagcaag tgccatcgct atgcatggac gtacacgggt
     1621 tcagatgtat gaaggaaaag cggactggga agtattgaaa gaagtgaaaa aacatttgac
     1681 gatcccattt atggggaacg gggatgtcaa aacgccagaa gatgcaaaat ggatgcttga
     1741 ctatgtcggt gcagacggtg tgatgattgg tcgtgcagcg ctgggcaatc catggatgat
     1801 tcaccgcaca cagcattatc tcgaaacagg agaactgatt cctgaaccaa caccagccga
     1861 aaaaattgct acagctaaac ttcatttaca acgcttagca gatctcaaag gagaaaaagt
     1921 agcttgtaga gaattcagaa cacatgcggc ttattattta aaagggattc ctcgagcagc
     1981 gaaagtgaaa gtagcaatca atcagacaga aaaacagcaa gaaatcaatg acctgctgga
     2041 tcaatttgta gaaaaagcgg aaaaagtact attgaaacaa acgttgtgat ttctaagttt
     2101 ttcgtgaatt ttcaagccga aattccaagt tcggcttgct ttttttgtgt ttcattggca
     2161 ttataggaaa gagattaaca tgagaatgga ggaacttttg tgacacagga acaacaacat
     2221 caagtagaag atttaaatga tcaaatgctt gtacgtcgtc aaaaaatgga acaattgcgt
     2281 gaagatggaa tcgatccatt tggaaaacga tttgaacgca cgcacaactc agaagaattg
     2341 catgaactat ttgatccaag aacaaaagaa gaactagcag aaatgggatt gaccgctagt
     2401 gtagctggtc gtatgatgac tcgccgagga aaagggaaag ctggatttgc gcatctacaa
     2461 gatcgtgaag gccaaattca aatttatgta agaaaagacc aagtaggtga tgaggcctat
     2521 gaagtattca aacatgcaga ccttggagat ttctttggtg taactggaca aatcatgaaa
     2581 acagacacag gcgaagtttc tatcaaagct tcagaaatca cgattttgtc aaaagcactt
     2641 cgtccactgc cagataaata ccatggctta acgaatattg aacaacgcta cagacaacgc
     2701 tacttggatt taatcagtaa tcgcgaaagc tttgaccgat ttatgaaacg aagccaaatt
     2761 atcagcgaaa tccgtcgcta tttagatggc aatggctata ttgaagtaga aacaccagta
     2821 ttgcacaatg aggcaggtgg agctgccgca cgcccattta tcacgcatca taatgcactg
     2881 gacattgatt tatatttgcg tattgcgcta gaacttcatt taaaacgact aattgttggc
     2941 ggaatggaaa aagtttatga aatcggccgc gttttccgta atgaaggaat tgatactaca
     3001 cataatccag aatttacgat gttagaagca tacacagcat atactgattt caatgatgta
     3061 atgaatctga cagaaggtat tatccgtaac gcagctgaaa aagtactagg aacagcaaaa
     3121 atcacatatg atggacaagc tgtcgactta gaaagtgact ttaaacgcat ccatatggta
     3181 gatgcaatca aagaacaaac tggtgtagac ttctggcaag aaatgacatt ggaagaagca
     3241 ttagctttag ccgaaaaaca caatgtagaa gtaaccgaag ctatgggtgt cggacatgtt
     3301 atcaatgaat tctttgaaac atttgttgaa gaaacattga cacaaccgac gtttgtatac
     3361 ggacatccag tcgaagtttc gcctttagcg aagaaaaatc cagaagatcc tcgattcaca
     3421 gatcgattcg aactattcat agtggggcgc gaatttgcta atgcctttac agaactgaac
     3481 gatccaattg atcaaagaga aagattcgaa gcgcaagaaa aagaacgtga actaggaaat
     3541 gacgaagctc acggtgtaga cgaagacttc ttggaagcct tagaatacgg tatgccgcca
     3601 actggtggtc taggcatcgg gatcgacaga ttagtaatgt tgctaactga tgcacaatca
     3661 attcgggatg ttttgctatt cccgacaatg aagcccatcg acaaataaac ccagtaaaat
     3721 caagggtttc cggctcctca aatccgagtt gacaacaaat tgacaacaat ttttcgtatc
     3781 aatacgaaga attacgacgc agaatgaata gttggcggct gaaacgcaat acagcacgta
     3841 tcgctgagag aacacttttt gaaagaaatt tcaaaggtgt tctctttttt tcgtttggtg
     3901 caactctctg tcgaacaaca aattgaatgg aggatttaat ttatgattag tgacaggacg
     3961 aaagcctatt atgatttgaa aaaacgaaat gatgtgcgag aaagtgcaaa gagactgcgc
     4021 aggcagttcc tccggtataa ggatgcagaa atcgtctata gcattacgca caagaaactg
     4081 ttggagctcg ctggtaaagc aggagccata tatcgaatgg atggaactgt tctgattaac
     4141 cgggatattt ttgatgaata tctggaacag tttcatgagc cgagtacctt ggcttcacag
     4201 gaggataaag aatgagcggg aatctatgga tgttttcgga tatgttggac gatgaagatg
     4261 cgaagatgat acagcatgaa tttgtcacct attatgaggg cgctgattat tacggactgg
     4321 gtctgaaagt ctttacaagg ttggcccatg aagcaggtgc ggtgtataag attggaaagc
     4381 gggtcttaat acggcgagat atttttgaag agtatctcag gatattgcga cgcaaagagg
     4441 atacatccga gatggatgaa aacatgaaaa gtgaggataa tgatatgaga caaggagcat
     4501 taacattaga tgcccagact gggcgaatgg atatccgatt tgatctggag aattattacg
     4561 gaggtctcca ctgcggagag tgtctggata ttcttatcaa tggagattgg gtaccgacca
     4621 gaattgaaat gggagacggt tggtatctga ctgggatcaa gacggacaat cttgaaggtc
     4681 tgattgtaag aatttaatag taacttatcg ccgggcggtt tctatttaca ggagccgccc
     4741 tttttttatg cccattttca aaagaggagg tgttccgagt gaagtgtctg atgaaatatc
     4801 agtgggtaaa gctgcccaga aatcgtctgc ctgagggcaa gggggtaatg ggcgcatggg
     4861 cgcgtctggc atcccgtgcg gcattccgca agggacaagc cctctactgt gggcatataa
     4921 acgctgtaag ccccggaatg tggtcaggag gtgtcgtagg gttaaagagc gtcctcggct
     4981 taaaaagccg tcagaaggct ctggaagtgc ttcagacgct ggctgaccta ggttatgtgc
     5041 agtatacgct tgactctaaa acgaaaaaac tgacatatca aattacagat tgggtagtga
     5101 aatgttccgg agaggaatgt atgcagggcg ctgtttatgc cacagaaggc tatggttttc
     5161 tatgccttcc gcgtaatatc acagaacgtc ttgcggagaa gcgatatatc tttgaggaat
     5221 cggacgcatg gatggatctc tggtgccatt ccgtatggga agacccgaac aatgcgtttt
     5281 cttttatggc tccaactgtc cagtatggga agtacggggc tttactcaca ttggaaacct
     5341 tgggacagcg ctggggctgg gaaaagacaa aggtatggag gtttatgaaa aagcatgggg
     5401 atgtctttgc cctataccgc cttccaggct cttatggctg tctcattttt aataaactgt
     5461 atccggcagg atcagaggtt tcagttccaa gccaggagga agttatgtgt attcttggca
     5521 aaatacgcat ttgtggtgca aatacgcaga agtcaggttc agaccatgag catatgaata
     5581 agctcatcac atggtatagc cgcagactga ctggaagtaa cctgtcagat gcagccatac
     5641 aggatgctga aaatcgcgtt gcattttcag accctataat acgcgcgtat ctttctccgt
     5701 gtaggaattg taagaattgt ggatatgact gccaaggtaa aggttatatg acacaggcag
     5761 tgattgaaac gagtaaaatc cgcgggccat gtgagccggt ggatatcaca aaaatagcaa
     5821 aggagtgttt tacatatgag caagcaggat gaaaaaaagc tgtatgaaca gcaggaaaac
     5881 aggatattgg cgcagtcaga cgcatttatt tcccttctta caaagcgagg gattcttgga
     5941 gatcaaaaaa ttgatgatga aaaggtgcgt gctgcccaga aggagaaaaa gcggaatgct
     6001 tatcacaata cccagatgct tctcaagaat taccggaaga tggtttgggt gttcagctgt
     6061 tacccggagg agattttgca ggaactggag actccgtttg agaactttga ccgaatggtg
     6121 gacaggctgg atctggagat ggccattggg aataaaagga tggagagccg tctggaagct
     6181 gcggcaaggt cgagactgtt gctggatcgg tttaacgagg caatggcggt tctccgcaag
     6241 gagccgacac ggggtgagaa gctgtatcag ctgatctata cggcctatct ggcgccggaa
     6301 aagctgaagt tggaggagat actgttccga ttggacctgt cacagaggca ttattacagg
     6361 cttcgtgagc aggcaatttc tattctttcc attcgtctgt ggtcggcgcc cagtgaagat
     6421 gtggatgtat ggctggagct gctgacgctc tttgaacatt tggaatgaca atttcagggt
     6481 aaacaaattg gcacaaaaat ggcatatgtt ccgccgatga caggggaata aagccgtgct
     6541 atacttgtat catcccaaac tgggattggg caaacaaaga tgccgtttct gaagctgaaa
     6601 tgcttttaga agcggcattt tttattgcct aaaatcaaag aaggaggtac tcggcatggt
     6661 taaacaaatc aaaagaggcg gagggcgcat gaagaccgcc tactggtcac tggttggtat
     6721 ggcgtctgca atgctgattt cgctgcagcc tgtcatggcg gacacaatct ggaaccgctt
     6781 ttcgacgatc atgaaggatg tgtacggcca gctggtggga atcagcacca ttgtggcggt
     6841 aacggcggcg gccattgcgc tgattgtcag gatgatttcc agaaaccagc gtgcagtcga
     6901 tgaggcaacg agctggctca aacgaattgt agtgacgtgg attgtcctca attccctggg
     6961 ttttgttgtt gcttatctgc aacccctcat tgccggcggc aattacacag gataaccaaa
     7021 aaagagaaag cagatggagg aggtgataat agatgcttga ttggattttt gaaggaatcg
     7081 tgacgtggat cagtagcatt gtttcacaga tgatggatgc ggtatctggt ttgttccttc
     7141 aagctttggg aacggatatg acagcgatgg aagaatactt tccattcgtg tctaaggcat
     7201 ttacggtcat gcagtacaca gcatgggcga ttctgttcct tgtaacggta tggcagcttt
     7261 tccgtgtctt tggaggacca atcacagagg cagaaaatcc gtgggtgctt ttggcaagag
     7321 gttcaatctt tgctttattg attggctatg caaagccgat tttcatgatt gtgctggata
     7381 tcgcccgtgc gccatatacg gctttgatgg agattaccat gtcggcagag gattttacct
     7441 ttgcaggtgt ggaacaggcg ctgtccaatg gtttgaccac gattgtagct atcgtatcgg
     7501 tggtagggct gctgcttctc atcattttag agattgccct tgggtggaat tattttaagc
     7561 tgttactgga aaccgttgaa cgctatattg tggttggcgt actctgttac acctcccctc
     7621 tggctttttc tatgggtgct tccaaaacaa cggcgccggt tttcaaaagc tggtgtcgta
     7681 tggtgggctc acagcttttg ttgctggtga tgaatgtatg gttcctcagg ggctttaaca
     7741 gctccatggg acagtatatc ggaaatggcg gtgcgctctc cacaggtcag ggcagtattt
     7801 ttctctggct gttctgtgcg ctggcatttt tgaagacggc gcagaagttt gacagctacc
     7861 tggcggccat ggggctgaat gttgcccaga ccggttccag catgggtatg gagcttttga
     7921 tggcagcgcg ggtgatcagc ggcgtgggag gcggtgtgaa aaatgcaggc agtatgttcc
     7981 atagtgcaag cactgcaacc ggaacgggtg cagccgcaag cggatttgct tccgggttcg
     8041 catcacgctt caagccaaat tcttttgtcc gtgacgcagt tgtggatggc ggaagccgta
     8101 tgggtgtcgg tggcagcatc ggttttgtcg gtcgtgcctt tggcggcatg gcggcacgta
     8161 atggcgctac tcttacagga gactccattt cttctgtagc ttcaagaaat cctggtgttt
     8221 ccgggacaat tgccggggac attgcagacc gcagccttgc gggctatatg ccccagctgc
     8281 agggccacca actgaaagac acccagatta caggaggtca gatcagtaca acttcaattg
     8341 gtgcagatgg aaagagcaca gcgcttgaaa tgtataacgc atcacagttt gagaaaccag
     8401 atgcaccaca ttccgtggtt acagcttctg atggaagcca gtggtaccag atggcaagcg
     8461 gagccggggc aggtgcattt tatgatgcgc cggcatttat gggaagcccg tcagaagcgg
     8521 cgccggttgc ggcggctttc cccgatgctt ccgagggaac aaccctcagg acggttggcg
     8581 atggcgtgat cgaggcaagc tccgagtctg gcaataccat gtggtacaac agtgcctact
     8641 atgatgagcc ggatgcgccg catacgatta tgcagtcggc aaacggtgta gactggtatg
     8701 cgatgcagca gcacgcagaa acacctgcct ttgagcaggg cgaggcagct tttgcctata
     8761 accaggcgca gttccagcag tttatgccgg gatatgaaca gcaggtatcc tctgtggatg
     8821 gatccgggcg taaggatggt cattttgagg tccgccatga aaatggatcc ggaaccatgt
     8881 tctacgatac atcccagtat gcggctcccc gtggggatta tcaggtgttt gaagatgccc
     8941 gcggaggcca gtggtatgcg attcgcggcg aagccgccgt ggaacgcaag ccggtatacc
     9001 aggatggcaa accggtttat gacggtgaca gtgtacggac agtctctgtg gaaactgttc
     9061 ggtataagaa tacgccatca cgctttggag agccaaaacc ccgcaaccat ggggagatga
     9121 aaccgccgag gcggaaacgg taaaataagt gcaggcagcc gaatcagaga gatgctggtt
     9181 cggctgtttt gcatatttca tgttgaaatt acgctgcttt tgcagcagct tgatataagg
     9241 aggtggaccc aatggctgag ccagaaaaac agcaaatcgg tgatggctct gataattatg
     9301 gacaggcagc cgggcaaatg gcaaaagcgg caaagcaggc aggccaggaa gcagcaaagc
     9361 aaacggcagc aaaaggtgcg gaggctacgg ctaatgcggc cgccgctaca gtaaaggcca
     9421 gtgtggaggg tggaaaggcc gccgcagaaa tcgctgccgg cactgccgca ggtggtccat
     9481 ggggcgctat cctgtctgcg gcatgggcaa tgcgccatac tctttttaaa atactggtat
     9541 gtatctgtct gtttctgttg ttcctgatta ccatgattgt ttccctgccg acgattgtat
     9601 caaacagtat tttcggactg gatggtacaa agccgacaga aggagcaacc ctgatgtcag
     9661 cctatacgga gatgtcaggt gccgtgtctg gtgtggtaga tgaaggatat aaccaatcgc
     9721 tttctcatgt ggatcagctg attacaaacg gtggatatga ttatgatctt tcgatggatg
     9781 cacttatcaa ttacgcacag agttccgccg gatatgatgt ctgttatata ctggcagctt
     9841 attcagcgtc catgcaacag caaaatacag gcaaggatga tatggtatca aagcttagag
     9901 gctgtgccgg ggagatgttc cccgttacat cagaagaaaa ggaaaaagag atcatcattc
     9961 ctgtttccta ttatacatat aagccgataa cacttaccgt agttacgaat aaggtgcaga
    10021 ccgggacgat taacggtacg ccccaatatc ggtatgatac ggcatcaaag acctactatc
    10081 ttccggatga ggcgcatagc agcgatacgg ctgtcacggt ggatgcctat aaagaggtgg
    10141 cggtggatat tcccgtttat tccggtggga agattacggg aacaaccacg gcaaactatt
    10201 atgtggctaa cgggcaggaa acactgaccc caggtacaga gatcatcaaa tatctggagt
    10261 gtaccattca tccatttgat aatacagtgg ttgccaaagc gtttggcatt gacctaaatg
    10321 caaaatacga tcagtttaat cttacttatg gcgaagtaat ccagaacatg gcgaatgcgt
    10381 taaaacggac gttgtatggt tctgtgagcg gcggacaggc ggttcccctg acggacgccg
    10441 agctgattgc ttttgtgaac cggcagaact gtagtgcgac aagaaagcat atcttgagca
    10501 ccgcgctgtc ccttgtgggc aaggttcctt atttctgggg tggtaagtca gcacccgggt
    10561 ggaacgatga atggaatacg ccaagactgg taacagctgc aggatcttct acgacaggca
    10621 cgatacgccc ttatgggctg gactgttcag gcttcagtac atgggtattt aatactgctg
    10681 tgggtgtaga aattggtgca ggttgtaacg ggcaatatcc aaatacctat gggatttctg
    10741 catcagagct tttgcctggt gatttgggat ttttagcaga tgatgacgga tggggacatg
    10801 tccttatttt tgccgggtat ggggagagcg gagaacgaat gtgggtgcat tcttctggtg
    10861 gacagggcgt tattctaaat acgccaagtt atgaaggaag cctttcctac cggcgtctta
    10921 gcattgtaga ttacgatgca ccggtattgg gtgagatttc aggaacaccc atttctactt
    10981 tagaagtgga tgtcacccat tactgtgcat gtgcaaaatg ctgcggcagt aatgcagacg
    11041 gaattaccgc cagcggcaaa caggcggccc gcggcatggt ggcaatgtca agctattatc
    11101 cgtttggcac gcagattatg ataaacggaa ccatgtatac cgtagaggat cgcggtggtt
    11161 ccggcattga aaatgacatc caccgtgtgg atatttttgt gccggatcat aacgaagcgc
    11221 tgcgtttggg cagatacaag acaacagcta cgatttacag aataggcagg tgagaaattt
    11281 tggatgaaaa ggagtacagc aatatttatg ctataccggc gaactataca gattccggca
    11341 agatttttgg cggaatgctg gagccgagga atgcagtaga gacagggata ttattagtca
    11401 ttttgggata tccggaattg atgttgattc ccatgtccgg aacgattcgc atcgtcgtta
    11461 tgacgctaac acttttacct cttgctgttc tttccatgat gggtattgac ggcgattcgc
    11521 tgtttcagta tataggacac atcattcggt attttgcaag caggagaaaa ttacattaca
    11581 gaagggtggg atatagatat gacccaaagc agctccgaaa aaagaaaaaa ggcagctcgg
    11641 caaagaaaaa aggcaaaaaa accggaaaag ctggagttcg ttcaggactt cattccggtt
    11701 aaaagtctcc agcatggtat tattgaaacg actgatggga gatatattcg gattcttgaa
    11761 attgagccga ttaactttat gctgcgttcc ggagaagaac agttcaatat catatcgtct
    11821 tttgccagct ggctgaaaat ttctcctatg cggcttcagt ttaaaagcat taccagaaag
    11881 gcggattcag ataagcacgt cgccatgatt cacgaagaac tggaagtaga ggaaagtgaa
    11941 gaatgccggc accttggaga aggttatatc cgtctgatta aggatgtggg aagcagggaa
    12001 gcgcttacaa ggcgcttctt tttaatcttc cagtatgaag aaatccgccg tggagatgga
    12061 gatgatttct cccagattta tggcatgatg cagacagcgg agcagaatgc ccgtgcttat
    12121 tttctgcagt gtgggaatac gattctccag ccgaaagatc cggatgaggc aacggcggag
    12181 attttatata tgttttttaa ccgtcggtct tgtgtggatg agccgttttc ctcccgcgtg
    12241 aaccgtgtgg tcgtggatac gatggtggca aaggataagg tcattggcgt ggatcccatc
    12301 ccgccgatcc gcatggcgca ttttctgtcg ccgcgcggca ttgatctgac ccatcataat
    12361 tatgttgtga tggacggatt gtattattcg tttctgttta tcaaaggaaa cggttatccg
    12421 ggtatggtac gggcaggatg gatgtcggca ctcatcaatg ccggggacgg gattgacata
    12481 gatgtgcaac tgcggcgtga gaatcggagt aagacaattg ataaagtagc acagcgcatt
    12541 cgcctcaacc gcacgaagct gaaaagcatg caggatacca gcaccgacta tgaggaattg
    12601 accaattcca tacaggcggg ctacttcata aagaatggca ttgccaacaa caatgaggac
    12661 ttgttctata tgtccgtatt cgttacggtt tccgccaaag gctatgagga actgatgtgg
    12721 cgtaaacagc agatcgtgga tatgttaaag tctatggaca tgtatgtgag cgactgtcgt
    12781 ttccagcagg aagcggcgct ccgctctgtg atgccgttct taaagattga gccgtcactg
    12841 gaaaagaaat caaaacggaa tgtgttgacc agcggcgcag cctctgccta tatgtttaca
    12901 tcttttgaaa tgtcggacga tacaggcgtg ctgttgggca tcaaccggca caataattcg
    12961 ctgtgtatcg tggatctgtt caatacaaag aaaaacaaga acgccaacct gaatctgctt
    13021 ggcaccagcg gcgcaggcaa gacctttacc atgcagcttc tggcgctccg tatgagaatg
    13081 agggggatcc agtgttttat cctggcacca atcaagggac acgagttccg cagggcctgc
    13141 aagaagatcg gcggcgagtt catcaagatt gctcccggtt ccccgcactg catcaacatc
    13201 atggaaatcc ggcataccat ctctccggag atggagctga ttgacgagat tgactacagt
    13261 gaaatggatt ccatgctggc tcgtaagatt cagcagctga tgacgttctt ctccctgctg
    13321 attccggata tgtccaatga ggaagagcag atgctggacg aggctttgat tcagacttat
    13381 gcaaagttcg gaatcacaca cgacaatgat tcgctgtatg taagtcgcag cgtctccccg
    13441 ccgaagatga aaaaaatgcc gattatcggg gatcttcatg aggaattgct gaaaaatccc
    13501 atgacgaagc gcattgctat tatcatcagc cgctttgtaa caggttccgc ccagagcttc
    13561 aaccaacaga ccaacgtgga cctgtccaat aaatatatcg tgcttgacct ttcggaactg
    13621 aaaggcaaat tacttccggt cggtatgttc attgcactcg attacgtgtg ggatacggtt
    13681 aaggcagatc gtaccaaaaa gaaagccatt atgattgatg aaatatggca gctgatcggc
    13741 gccagctcca accggatggc ggcagagttc tgcttaacta tcttcaaaac cataaggggc
    13801 tttggtggtg ccgccgtctc cgcaacacaa gacttgtccg atttcttcgg tttggaggat
    13861 ggcaagtatg gacgtgcgat tatcaataac agcaagaata agattattct gaatctggag
    13921 ccggatgaag ccaagtatgt gcaggaagtg ctgaaactga ccaaaacaga gatccgttct
    13981 gttacccggt ttgagagggg cgaagcattg gtctgctcta acaataacaa agttcctgtt
    14041 gtaatcaaag cttccaaaga ggagcaggaa atgattacaa ccgaccgggc tgagttggag
    14101 gcgcttttaa aggaacgcca gcaggaacaa acgcattctg cgtagaatac gcattttatg
    14161 aaaaatacgc acaaaaagga ggtgttgcct atgctgctgc aagatgagca gtatgttgta
    14221 aagtggctct cacaatatgg agctttgcct aaaacccaga ttacacgaat gctgcaaaag
    14281 ccagcccaga ccgcagataa aatccttcgg aacctgaaga agcagatgcg gattgcggat
    14341 gtgtcgagcg gctattatat tgggctggat gcgatggata aaccggatca gcggaccatt
    14401 cttgccgtat gggttctttt gaaattcatt gattatgttg acccaatggc gcattatccg
    14461 gcggtttatc catctcagct ttttttctta aaggagaata tcggttacga aatcgtggtg
    14521 ttatatgaag gcgagcagaa tcttttgaaa ctgctccaac ctcaggagga tttgaagtat
    14581 attattgtcc ttccccgtat ctctatggca tacgggatgc ggcttcctca tgcaccctgc
    14641 ttgtttgcaa ctgtggattt tcagggggat gaggagccag gggttacttt ctattcagag
    14701 gaggcggtga gcgatgataa aatttctgga cacgatgggt aaaatcgtga gattggaaaa
    14761 gcgattgcag gagcagcttg cttctataca atggttttgt gaaaatggga gcacttatga
    14821 agtttatctg cattcactta atatggaaga aacagcggag cggctggttc ttttgacacg
    14881 ggcgcttccc gcttataccg gtgtgccgaa tgcaaagctt gaggtagagc gtcggataaa
    14941 ggattgtatt cccgtagaaa tcggttttac ggcggagggc tggttttgtc tccgcatccc
    15001 ggctttgctt ccgaaaaagt cgggcgggtc ggcggattat atccgctctt ttttatatcc
    15061 tgctatgcgg gccttttttg aaaagcagct tcctgtgcgg tatcgggacg gtatcctgat
    15121 ttatcgtcat gtatatgaca aagccagacc cgaaagaaaa aagcgggatc atgacaacat
    15181 tgaaatcaat atggtttcgg atatcgtagc catgtatgtg atgacggacg atggcccatc
    15241 ggtctgctct cattattact gcagcgcaga aggatcggag gaccggactg aggtttatgt
    15301 agtgccaaaa tgggacttcc ccatttggct ggtaaaggaa acagggatac cggatgaggg
    15361 ggtactgctc tatgaaaaaa ggtaaattta accgaaaaaa catatgtaaa aacgatgctt
    15421 atcgggcgga gaaaaaatac atatcttctg agagggcaaa aaagcccagt atagcagcag
    15481 tttgggggcg tcattacgcc ggaaattccg acaaagagag cagtcatgtt gtcggaaaat
    15541 gcttatgagc ggaggtgagg gtctatggtt catttccgcc cgggcagtca ggtctggcag
    15601 atcatcacgc tcctttcatt tgtgggggag ttcccattca aaagcctctc tctgcttggg
    15661 cgtgaacggg tctataaggc tcttatttcc agactgacta cgcttcagac gattcgtaat
    15721 ttcaattccg gtgacgaaat cacctgccgt ctcctgaccg tatccgggaa aggggcgggc
    15781 aagactatac ggctctacaa gggtgcgctt cccatactgg aatggctgta tccgggtgtg
    15841 tatggatatt atatggattc attctgggga caccgctttc caggagacgt atcacaccgg
    15901 gacaggaacc accgggttgc ggaagctgcg gcgatgttct tgaaagccgg tatggaggca
    15961 aggccatatc ttctccccag gcttcagaac agggagattc tgcaggttgt gcatggtacg
    16021 ccctgttttt atctggctaa ggatctgaag aaagttgggg aagcggagat gaacaagacc
    16081 atgttcaccc gcatggccgg cgcattattt tcttccggca gatgttatgc cgtttataat
    16141 acgagggatg ccgtgatgaa gtggagcggc atgggtgagt ataaagccct ccacagtctg
    16201 atcgagctgg caagactgaa tgccggtatt ttggaggtgg attcagcgat actgtttggc
    16261 cagtctggag agacagcgct gcgaaccctg ttagaatcag acaaaaccag acggctggag
    16321 tttcgattcg attccattta ccgccatgtc cattttatcc caatgaacgg ggacggtatc
    16381 cggcagctgc gtcttctttc tgcaccagat tggaaagcgc agctccttga acttttattt
    16441 gagcctgagg tcagatccta tgaccgaggg ctttttgaat acgacgcctg tgtgaatggc
    16501 gtaaatatct tatcgcatct ggatggggac attgcaagac tcattcgctt tcgggatgca
    16561 attgaaaacc aaaccggacg gtttgaggtt ctatgctttc cccaccagac gcacttcctc
    16621 cgggaatact tgggcgggct tgccagcatc aaaaccatcg gaatggactc ggtggaagcg
    16681 gagctttgcc cagagaggag gaatttgttt gaaagataaa aaaaggacag tcggcattac
    16741 catggctgtc atactgggat gcacagcgtt tttctatctt ggcggaatgc ttggccaatt
    16801 gttttcaaat tacgcagcct ggatgcaggc ggatgggctt gctggtgagg cggctatgaa
    16861 accagtcagt tggaaccctg tggtgtgctt cccaatggcg tttacctcag acgggctgaa
    16921 aggattgctc ggaatcttgg ttcttggcgg aggagtcttt gcatatgtca agcttcatga
    16981 taagtttgac ggaaaaagct atgaccccag aggtttcacc aaaagcaaga ccggaattta
    17041 cggaaccgcc tcatggatgg aagaaaaaga aatgcacgag gtgctggagg tgtcctctgt
    17101 cgataaagca gaaggaacga ttctgggtga gtataaggga aaagcggtct gtatgccaaa
    17161 ggacaccaga ctcaaccggc atatcgccat attcggtgca tcgggtacca tgaaatcgcg
    17221 cgctgttatc cggaattcat tatttcaggc gctgaaacgt ggtgagtctg tgattatcac
    17281 ggacagtaag gcagaattat atgccgatac ggcagagatg taccggcagg caggctatga
    17341 ggtaaaaatc tttaacctgg tcacgcctga gcatggggat tcgtggaact gcatgtcaga
    17401 cttaaacgga gacacgctga tggctcaggt actcactaac gtcattatcg gcaataccag
    17461 ttccggtaaa ggcgaccatt tttgggacaa tggcgaagga aacctgttaa agtctttgat
    17521 tcttctggta gaccaggaca gaacccgaag tcccgatatg aaacacctta ctgcagtata
    17581 tcagctcctg acccagaaca gcgagcggca gctgacggca atgtttgaca agcttccgct
    17641 tgatcatccg gcaagggcgc cttttaattt attcgctcag tccagcgaca ccgtgaagtc
    17701 cggcattatc ttaggactcg gcacacggct gcaggtactg cagaaccagg cagtgcagcg
    17761 gatcacaagt cagtctgaca ttgatttgac ttcccctgcc agacacaaat gtgcatatta
    17821 tattatcctg agcgaccagg acgccactat ggcattcctg tcatcgctct ttttttcgtt
    17881 catgtttatc aaactgactc ggtatgcaga cagtcggcca aatggccgct gcgatgtgcc
    17941 tgtaaattta atcttggatg agtttaacaa tgtgggccgc attggtggtg cagaagacgg
    18001 gtcggatttt gcccggtctc tttcggttgt ccgttccaga gatatccggg tcatgctggc
    18061 agtgcagagc ttgggtcagt tacagaaccg gtatcccaat aacctgtggg ccgagattat
    18121 cggcaactgc gatatccaat tgatgttggg atgtaccgac gaggttacgg ctgagtatat
    18181 ctcggcaaga tcaggggata tgtccgtaca ggtaaattct actatgacag tgcggcaaac
    18241 cattgcaatc gctcaggtca ttccccagta tcgtcatact gagggacagg gacggagaag
    18301 acttctgact ccggatgagg tcctgcggct tccaaacgaa gaaatgctct gcgtcatacg
    18361 aggctgtaac ctcttgaagc tcacaaaact ggattttaca aaacatccca tgtcgaagca
    18421 gattgtgagg acatccatca tggattaccg gccggcaaca atatttacgg caccgatact
    18481 cagtgaacct gtcccagtgg cagagccgga gaaaaagccg gcacgcaaaa aaagtcttta
    18541 cagttcggca aaaccgccgg ctgaatttta attttacagg aggtactaac tatggcaaca
    18601 aaaaaacaag aattgctccc tgaggaggaa gttctgcagg aagcagcccc tcagactgat
    18661 gtgaatccgg agcttgagga gatgtctcag cctgaagaca gctccccgga tacggcactg
    18721 ccccagactg actctgaaac ttcaacagaa ggcgcctctt cagaggaatt cagggatgca
    18781 gaagcggcag aagctcccgt ctcccagatt gatatgaaaa caaatgacgg gcagccgcct
    18841 tcggagtcgg cagaagaaat atcttcccag acggatatgg agagtgccga agacgacacc
    18901 tcttcagaaa acgcatcgga caaaactact ccccaagagg gctcccccca gactgagccg
    18961 gagaaagtga aaaagccccg cgggcgtagg aagaccaagg aaaatccatc ccagtctgac
    19021 acagaagcgg attcagggga agagaatgca tccgacggcg tcgaaagggc gcctaagcct
    19081 aaaaggaagc gtgcgtctgc cagaccggtt tcgccggtgc tggccattga tgaccagctc
    19141 agcgtcgaga cagatgcaga aaaagcaaag aacgatttgt tggacttact ggagtcccag
    19201 aaaaccggcc gcattctgac cggaaccatc cagggtgtgg agcgccccga ggacaacccc
    19261 tcccgttctt tcgcagtcat ttaccacggt gagttcaaag tcatcattcc tgcagaggaa
    19321 gcggtggagc cgccagcgga ttatcgcggc aggcttccgg aggacgtgct gcattacatg
    19381 cttaccaagc gactgggcgc ggaggtggat tatatcgtca aagggatcga cccggcatcg
    19441 ggcattgcgg cggcgagccg tttggatgcg atgaaggcaa aacgcaagca gtattatctg
    19501 ggtacagaca gggatgggaa caacctgctc tactcaggtg tgtgcgcaga agccagaatc
    19561 gtgtcggtga ttcgtgccgg catttttgtg gacttgttcg gtctggagat ctatattcca
    19621 ctcagagaac tcagctatca gaggtggatg gacgcagcgg cccatttcca gccggggcag
    19681 cgtatcctgg tgaaggtgct ggaggtggac aggagcgacc ggaaccatgt caaggcaaca
    19741 gcttcggtca agcagtctgg tgagaacccg tatgaaaaag cgctgcgcag gtattccgtc
    19801 ggcaaccggt atgtgggtac ggtcagtatg gtggacacca acggcgtatt tgtggcgctg
    19861 gacggcggca tcgactgctt gtgcagttac cccaagcgcg gccgcccgcc cagagggtcg
    19921 agagtaacag tacgcatact gggaatcaac catgattcca accgaatctg gggtgccatc
    19981 acccatatcg cagcgcccag ataatatagg aaactcaatc tgagagaaag gaggaaatcc
    20041 atgaaccatt gtgatctcag cccggctgag cagaagaagc gcaaagaaat tgccgagcat
    20101 acccgaaaga tgatcctgtc cgaactcaaa attgaaagtc aggacgacat atccctgatg
    20161 gcgggatacc gggattatta tctgcagatt tcgttttctg agcttcatcc acttttggtg
    20221 ttctgtttgg ccaaggcact tgagaaaccg agcactgaca gacagaggca gatgacaaac
    20281 gagctgaatc tgcacagcat ccttggcagc catgccatca atgatgaggt cggttgctat
    20341 tcttaccgtg cgacccagtg gcttgacgca gaactagatg caccgcgttt ttttgagata
    20401 cttgatcgtt gcgtcgatga ggcggaccgt gggttctaca agcttgcggg ttaagatgcg
    20461 ttgtatttct ccatactcag tcgtgtaatg taagtaaaaa cgacaaaagg aggaatgcgt
    20521 catgaaaaaa tgcgtattat tcgtggtact ggcagtgctt ctggcactta cggcctgttc
    20581 caaagatgtg ggtacttcgg atgaccatgg gcaggaaagt aaacataccg ctcagtctca
    20641 gacagaaaaa gaatctgaag atgaggacct gaaggagact gctccaatag aggaaccaaa
    20701 accagtttcc cagagtgagg cgaaagagga ggtatccctt tcggatccgg ttcatgaaac
    20761 ggatcaaaat tctgagtctg ggaaagaggc gcagaaccgg gcagaggaaa atgctcccga
    20821 accaaagcag cctgccgcag gcaatggaca gcccacgccg gagccaaaac aaccggaacc
    20881 acccaaaccc acagaaacac cggcagagcc gaatcaggaa gaaactcctg tatctgccac
    20941 tgaaccggag tctccgaaaa ccatctatga ttatgcgttt gatgcagagg cgattcgctc
    21001 agagctgatc gcacttgggc agtctatggg tctgacacat attacggatg atgacggaac
    21061 tccatgcacg ccggacacct gttcctgggc ttctccggta acagcgtccg aatcttttca
    21121 gggcaatagc ttgaaacggg cgcttcaaaa ctatgtaacc tctatgccgt ctaccattgc
    21181 ttcttatgga ggaacgcaga ttacctgttt taccatttat gtacgggaca acggaggagg
    21241 aagctataca ttttattttc tatactaagc ataagcctgg ctccctgtat gatacgggga
    21301 gctttttttg tgcccaaaat tgaaaggaag gtaaacctta atgaagaaga tcatcaaaga
    21361 aaaaggcaag cgcctgcttg ccggattttt aagtctcctg accgtcatcg gcttgatacc
    21421 gacgacggtt tttgctttta caccatcgga gggacagacc gtatcctcct tttatggcga
    21481 ttattatgtc agcgcggacg gaaactatta ttattccgca agttcataca gttttctggt
    21541 atatgacagc gatggcaata cctccgtgca aaccatctct gccggaaatg ccagaaaaaa
    21601 atacatgatt tccaatggtt cagagagccg attcgtgtat tgtattgagt ctggtattaa
    21661 ttatggtaca tctgataatg gatatagatc ccagagcgga aagaacagcg catattttca
    21721 gaacctccct tacagcgccc agtatggtat tatgctgacc agcgtgtatg gatggcagcc
    21781 ggggaaaagc tctcctgtgt caggcgctaa cgaggatgac tatgcggtgg ctacacaggt
    21841 tcttttgtgg gagtaccagc agcagctcag aaccagtccg caggatctgc acgccaattc
    21901 ggcaggtatc cgtgcggaca actacctgcg taccattcag ggccgcccgg cggaacagtg
    21961 ctataactgg attttaggtc agatgtcgca gcatacgacc attccgagct ttgcatcggg
    22021 caaaagcagt tctgcacagg tacatacgct aaagtatgac ccggcaacca agaaatactc
    22081 cctcacgctc acagatacca ataatacgat ggcagatctt aaattcagca gcagtgatgg
    22141 gattacggta agccgttccg gaaacaagta taccttcacc agcagcaaaa tgattaccag
    22201 cccggtatcc atcagcgtgc agaagaatgt gcctggggtg aaaaacgata tgttgatctg
    22261 gggccgcgtc ggtttccaga cgatgatgtg cggcgccgat gatccggtag tattctacat
    22321 gaagattgat acggaaacct atggtaccgg gcgcatccgc aagacctcag aagacggcgt
    22381 ggtatccgga atcagtttcc atatttccgg taatggtgtg gacaagactg taaccacaaa
    22441 ggaagacggt acagtggata tccagctgat gcctgggact tatacagtaa cagagcagtc
    22501 gatagaccgc tatctgccgc aggatactca gaaaatcact attgccagcg gacaaacttc
    22561 aacggtaaca tttaacaata agctcaagcg cggctctctg gaggttatca aatcttcaga
    22621 agatgatttc gtggaaggcg ttacattcca tttatttggc acttccctcg caggtttgcc
    22681 cgtagatgag tatgccataa cagatgcttc cggtgtagcg aagtttgaaa atgtcttgat
    22741 cagcggaaat gaaccttaca ctgtggaaga agtcgatacg gcaatccgtt atgtcgtacc
    22801 ggatgatcag acagcgccga ttcagtggaa acaggtgaca aagagaaact tcgagaatat
    22861 cctgaaaaaa ttccgggttg aagtggtcaa aacggaccac gtaaccggct atgctcaggg
    22921 cgatgccagt ctggcagggg cggtgtacgg tctgtatcag ggcgatgagt taatcgcttc
    22981 ttacacaaca gatgccaatg gctcctttat cagtgattat ttcatttgtg attctaactg
    23041 gacgctccgt gaaatcagcc cttctccggg atatcttttg gatgagacca tttatccgat
    23101 tccggcagaa cctggtaatt ttacggttga actgaaccaa attccgatgg aagttacgga
    23161 acaggttatc atgggtcgta tccgcctgat caaacacatt gattcagaac tggaggatac
    23221 tgaaagcaca cctgcagcga gagaacagga agcgggtatg gtaatgcttg cggccgtaag
    23281 cgtggacgac cagcctgaag aagccgtaga cggcgctgat gtccagactg aggaaactgt
    23341 aagcggcaac gatgcccaga ccgagacaga aaacacagaa cctgaagtaa gcgatgaaga
    23401 ggctcaggaa cctgaagaat ccggtacaga agaaagtgct gtcccggaaa ttgaaccgga
    23461 aacgaatcct tatgagccgg atccggttcc tatggacgaa atcgaagcat ctggacatga
    23521 aggtataatc gaacagcctg agaaaggtgc caagttccag atttaccttg ccagcgcagg
    23581 cagctttgaa aatgcccgcg aagctgaacg agacgagctg attaccgatg cagacgggtt
    23641 tgccatttcc aaagatctgc cttatggccg atatgttgtc catcagactg agggcatgga
    23701 gggacaggca tttattccgg atttcacggt gtttatccga tctaatgagc agacgtattc
    23761 ctatatccta aacaacttga cccagtcaag ctttatccgc gtggagaagc atgatgcaga
    23821 aaccggaaaa attattcctg cagcgggagt cggtttccag gtacgagacc tgtccacagg
    23881 agaaattatt tctcagacag tgtattatcc gacacctgtt accatcgaca catttttcac
    23941 caatgatgag ggctggctga tgctcccctg tgagctttcc tacggccagt atgagctgat
    24001 tgaacaggaa acctgttttg gttatgtttt ggacagtgag ccggtgcctt tcacggtaga
    24061 cggcacacag gatgtggtcg tggtcgaaaa gcataatatg cctcagaagg gcatcatcca
    24121 tatcacaaag accggcgagg tctttgctac cgttgcggaa atggacggtg tggataagga
    24181 tggcgcagag attatgtacc agcctgttta tgaaatccgt ggacttgaag gcgcaaccta
    24241 tgaaatccgt gcagcggaag acatctatac gccagacgga accctaagag ccgcaaaagg
    24301 cgatgtagtc gataccatta cgaccgccgg aaacggaatt gcttccagca aggagctcta
    24361 tctggggaaa tatgagatct gtgagactga ggcgcctttt ggtatggtac tcaatcagga
    24421 aagcaagcag gtagagcttg tatatgccgg ccaggagatc gacgtaacag aagtttcttc
    24481 gggcttctac aatgaacgcc agaaagtcca gatgagtctg gagaagatgc ttgaggtgaa
    24541 cgaagtcttt ggcatcggca ataacaatga acttaccaat gtcacatttg gcctgtatgc
    24601 ggcggaggac attaccgcag cagatggcgc tgtcatcccg gcagacggcc tcatcgaagt
    24661 tgtcggcatt gatgcagacg gcaacggcgt ggtcaaaacg gaccttcctt ttggaagcta
    24721 ttacctgagg gagcatagca ccgacgccca ttatattttg agcgatgaga agtatccggt
    24781 cacattttcc tacggcagtc aggaggacgc cgttattacg gtgaaggcca acgatggcga
    24841 agccattgtc aatgagctga tctatggctc tgtatccggc atgaaagttg atgaaaatgg
    24901 agacgcacta agcggtgcca agattggctt gtttgcacct tccgttacag agtttacgga
    24961 ggagaatgca atccttgtaa caacttctgc agaggatggc gctttctcct tcaatgatat
    25021 accatacgga aaatggatta tccgggagat cgagcagccg gaaggtttcg tgctttgcac
    25081 agagcagttc ccggtcgtaa tcaacgaaga cgcgcaggta atcgaagtgg aaatcaccaa
    25141 tgagtttatc cgcggcaacc tacatctgac caaatttgac aaggactatc cggagaacaa
    25201 gctcagcggt gcagtatttg aggtatatcg tgataccaac ggcaacaaag agcttgataa
    25261 agaggatgag ctgcttggtg taatggaaga gacggagccc ggtcagtatg agatgaaaga
    25321 tctcttgtac ggcggtgtgc tcatcagaga gaagacagct ccggacggtt tctaccttga
    25381 tgaaaatacc tattatatca tgattgacac cgatggaaat acctatgaag tcgagaatga
    25441 ggccggcaag ggcttctata atcaggcgtt cagaggcaac ttaaagattg tcaagacttc
    25501 ttccgatgga aaagtggaag gcttcagctt ccgcattgtc ggtgataatt atgacaagac
    25561 gttccagact gatgcaaatg gtgagatttt tatcgagaac ctgcgtgtgg gcaagtatac
    25621 cgtgactgaa gtggaggaca gcgtcagtgc aggctacaag catccggatc cggtaacggt
    25681 agagctggtg aaagatgaaa cattgacggt gaatgttcat aacgataaag tcactgtgga
    25741 tgtgccgaag acaggcgata acttcaaccc gtggatttgg gttggcttga ttgctgccag
    25801 caccgctgga cttggtgcaa gcatcttctg ggcaaaaaag cgcagaaaga aaatggcaga
    25861 gacagcagag taagaatatg taaatgaaca taaaggagtc gggcaggaat tttctgcccg
    25921 attctgattt taggaggtaa tgaccatgaa aataaaattg ttaaaagggg ctttgacagt
    25981 cctctccctc atctgtatcc tctgtattcc ggttacgccg atctatgcgg agccgcaggg
    26041 gacagatggg acggaaatgc aggtcatgga gcctgagaaa ctggagattc agcttggtgc
    26101 agactgggct ggcgtagagt ttcagctgaa aacggattcc gggctgtatc ccggtaccat
    26161 agccgtcgga gaagatggtg ttctgagaat ggaactgggc ggcagcaaat cttatgtgct
    26221 gacttgtatg aattcatccg tatctgcccc agaagcgacg caggcccctg ccacaacaga
    26281 ggaagcgcaa aatgagagca atgcgggaca ggaaagcaat gaatcaaata gcgggcaaga
    26341 tacagagggc acagttgccg gtatcccaat ccttcatctg gtactgtttg caggcggcat
    26401 ggtcttggcc gtcggcagcc tggtcgcaat gcgggtgatg aaaaaacgca gagaaagtga
    26461 tgcggagtat gaggatgaag atgaatatga ggatgattag tcccagactg agtcccaaat
    26521 acacgaaaag ccctaaagga atattttata taaaaaatca aaataaatat tcctataagg
    26581 cttgacatac taattcttat ttgctatact ataaccgtgg caggggcttg cgttcaggct
    26641 ctcggtacgt ttgtggtata gcgtcagacc tgattcaagg gaatcaggga accgttacgg
    26701 attctgcgca ttgagcggat ccacaacaac gcaagtcaat tgaatattaa agccggaagg
    26761 ctgcttaaat acttacagag tcaatttgct ggatgagaca ttgaaattcc agtgccttgg
    26821 ctctgtatgc ttttttgcag accttccggc tttttctttt atccaaacgg aggtaagacg
    26881 atgaaaaaac aaaaaaagaa aagaaaggga tttcaggcag gttccatgcg atgcccttat
    26941 tgtggcagcc cggttattta tagaagtgct gatggaatct atcatgataa cagtaaaaac
    27001 accatgctct atgtgtgcag tcactatccg gaatgcgatg cctatgtccg tgtccatgcc
    27061 ggaaccaata tcccggtggg aagtctggcc aatcatgagc ttcgcactct cagacgaacc
    27121 gcacatcatt attttgacca gctgcaccag tctggaatga tgagcaaaca agacgcctat
    27181 cagtggttgg cggatttgat ctgcgcacct ttatcggagg cgcatatcgg ttatctgggc
    27241 gagtattact gcaagcaggt cattgaggag agccgcaggc tgttgacgcg ccagaaatcc
    27301 ggaaagaaca ggtgccggtt tcaggcatta gaagggagag tggcggcttc atgacactga
    27361 ctgagaaaga acggggaaag attgaaaaga atatcggtct ggtacataag gtgattcggg
    27421 ataagctgca gccgccatat caagtaggga tgtatttcta cgaggatctt ttccagatcg
    27481 gctgcattgg gctttgtaaa gcagcatcaa cagacaaagg cgggacgttt tcgacctatg
    27541 cctaccgttt gatctggcac caaatctgtg atgccatgat ttatgcaacc aggcggcaga
    27601 cgaaggagac cacttgtgat gtgacgccgt acattgctgc aaaacaggaa aacccagagg
    27661 agctgtcgga tttccgcatg gatatcaaac gggcactcga acaggcaaag gctgatgcgc
    27721 cgccttccac caggaaaggc atagatgcta tctgcatgat gtcgaagggg tatacaagca
    27781 gcgaaattgg agaaaaaatg gatgcttcgg caaagcttgt gtgtgcatgg gtatccaagg
    27841 cacggaagtt tttgaagagc cggccggagt ttgcacagat tgcagctgcc tatcatttgg
    27901 aatgatgacg ggcagaacat aagggatgct gacagccttg tttggttttc agcatcccga
    27961 tttttttatt ttggagggaa attatgaata taagaaaaaa gaatatctgg atatggctga
    28021 ttccatttct tattatggct cttacgctga ttttatttaa ggcagtattt ttgttgggtt
    28081 atgtgccaac agagtccatg gaaccgacgt tgaaaaaaga cagctatatc gttggaaccc
    28141 ggattttctc caaacttgaa ataggcgata tcattatttt ccatcatgac gggaagcttc
    28201 tggtcaagcg cattgcagcg gaggaggggg aagcgataga gcataacgga gtcggtctga
    28261 tggtaccgga ggggtgctat tatgttcttg gagacaatgc ggagcattca ctggattcga
    28321 ggtactggga atatcctttt gtaaagcaaa aggatattgt ggcgagattg ttttggccat
    28381 aagatttttt actccgcttc atacgaaaac agacattatg aaataggctg tatttgaatt
    28441 gggtagcagt gtagttttga cataataaat gaaaaagaat aaatatggga tatttcgagg
    28501 aacaatctag gaacacatag aagatgatag taagaaaaaa gcagccaacc ggcgtggccg
    28561 ctttacttct tgctgttcag ttccttcata atgccgagga gataagccat cgacttggga
    28621 tccagtttct ttccctcttc gataaattcc tgcagagcct ccggataagg atttccttca
    28681 tcgaagaact cccgcggtgt tacgcccaaa tattcgcaga tgtaaaaaaa ggattgcatg
    28741 gctggaagtg actttttatt ttcgatgtta ttgatgtagt tgtttgcctg tcccaatgac
    28801 aatgacatgt cgcgtgcaga tacacccttt tgtaatcgca gttttgccag ccgttccggg
    28861 acgaaatctt catacatagc ctttacctcc cattctctaa tagattgtac ctcacacata
    28921 tgatttattc gcaaaaataa aaggggtgtt tacgttggcc taaaaaaata aatctattat
    28981 aattaggtaa aatgaaaaaa taaaactatc gagcgaggga gatacaatga aaggaaagac
    29041 atgttgtatt accgggcata gggatgtgcc tgataaagag attaacagca taaagaaggc
    29101 tctgcatcag gaaataaaaa aagcagtgaa ggacggcttc actgtctttc tgtctggttt
    29161 tgcagatggc gtggatcaat attttgctga gattgttttg gaactgcaga aagaaaacag
    29221 gaacttaaaa ctgattgcag tgcttccata ccgtgaccgt gtgaaaggat tgaatcacag
    29281 ggtacaaaca agagctttgc tgagtgcctg ctctgagatg gtggtaattc aagaggaata
    29341 tcagcccaac gtctacttac agcgcaatcg gtatttggtc gagcattcag accgcgtgat
    29401 tgctgtgtac gatgggaggg agaaaggcgg tacggtgaaa acaatccgtt ttgcacaccg
    29461 cctccagaaa gaattgcgtg aaattccggt aggactaaac ttggacaaaa acaggataaa
    29521 tcttggacac aataatgtat aaaaagagtg gagctgcagg cagttccatg atataataaa
    29581 acctttaaaa atgaaaattg tctgaaaaat gatggaatgg aggtcattca tgaaaaaatg
    29641 gtatgacgaa gaatacgaat ttgaaattga ggtaacaggg tttcttcgtg gtgatcacac
    29701 agagcggtat tgccgaaacg gagaggaagt cggagataaa tacacctgta cttatggatg
    29761 ccctgtaaat gcggatgggc aaggaatttg ttctaaggta atgataatgc tttttcctat
    29821 tatggaggca gtcagaagcg gcggggattt agaaaacatt ggcggcagcg gtaaatactg
    29881 caaggaaatc gtatgcccgg atggatgtgt gatgttccgc ttgacagcgc aaaaacttgg
    29941 aaatgagaac ttctttaaag gaaagttttt tgattagaaa aagataaaag aagggaaatc
    30001 ggatatgctt gataaaatac caaattttga cgatatgatt gccttggtcg gacagtcttt
    30061 atatgacgtg tggcaaaagc tatgctctgc gattgacgag caatacgata tggactgcat
    30121 ttggaacagc ggcggtaagg catggaccta tgaatacaaa taccgcaggg gcggaaaaac
    30181 actctgcgcc ttatacgcaa gagagaactg cattggtttt atggtaatct tgggaaagga
    30241 cgagcgggca agatttgaag ctgatcgaag tgagtattct gaaagtgttc aaaatgttta
    30301 cgatgactca aaaacctatc atgatgggaa atggctgatg tttgagcctg ttgatacatc
    30361 tttgtttgga gacttcatgc agctattagc cattaagaga agaccgaaca ggaaatagaa
    30421 gtccgtggag gagaagatgg cgaaaaaaga tacacaataa aggttgctga cagctgcgag
    30481 aacagagact taacagatag tatggtgatt ggcacatcgg cgatcacaaa tgtggaaatg
    30541 agtagccttt caatttcacc atacgcagat ggatggtgtt catcatgcgg gtaggttttt
    30601 aacaaacctg caaaatgaaa tcaatggatt gaaatagaga aatccgtatt acatattgga
    30661 tttaaggagg ataaaaatga aaagggaatt aggaatcgct cgttgcggat tagcttgttg
    30721 tttgtgttca gaaaactgtt ccggctgcaa ttcaggagaa tgtccggaca gggaatggtg
    30781 cgaaaacagg aaatgttctc tttccaaaag tatagggcat tgttatgaat gcgaagagga
    30841 atgcagaaaa ggcttgttag ctaaaatcaa gccctatgga ttcacagaat ttgtgaaaaa
    30901 atatggtgag aaagagttat tggattgtct tgaacgaaat gaggcaaacg gaattgtcta
    30961 tcatcgtaac ggtataatgg gtgattatga tgattttgat gatgttgaaa agcttatgga
    31021 ttttataaaa acaggagaaa gataatgtcc gatttatcgg ataaacctta ttagtcatca
    31081 gcagttagaa ggagaatatg tttatgagtg aaaaattccc gcaagaagcg aaaaagataa
    31141 tggatgaaag atttgggcac gataccttac tgtcacttgc cactacggat caaaacatcc
    31201 cgtatgtgag aacggtgaat gcttattatg aggatggctg tttttatatc atcacatacg
    31261 ctgcctccaa caagataaag cagataggta ataaccccat agtagcggtg gccggcgaat
    31321 ggttcacggc gcatggcaaa gggataaacc ttgggtggtt ctgcaaaaag gaaaaccatg
    31381 aaatggccca aaagctgaga caggcattca gcgaatggat tgataacgga cacaataatt
    31441 ttgatgatga aaatacgatc atcttatgtg ttcaattaac agagggaatt cttttctctc
    31501 atggcacgag atatgacata gatttcgctg gtaactgatt gcaacctgca gaagccatgt
    31561 ctgtgctatt gtaattggaa agggggactg tgaatcagta tgttggattt gaaggatata
    31621 aattatatgc cagatatatt tgaaataagt gattacattg gtaatccgct gtttgatcag
    31681 ttttgtgaat ttatggatag agaatataaa gcattgcgta aaattgaata cagcaaggac
    31741 gtttgggccc gcggatggaa tataaagttg agaaaagcag gaaaatcttt atgtgtaatc
    31801 tatcccaaag agaattactt cactgtgctg gtggttatcg gcaataagga gaaagaaaag
    31861 gtcgaaggaa tattgtctca attatctaat gaggtgcaga caatctaccg tgaaacaaaa
    31921 gaaggcaacg gacaaaggtg gcttatgatt gaactgcatt caaatgattg tatatatcag
    31981 gacaccttga aactgatccg cattcgcaga gagagcaaat aaaaaaacag aatcattgag
    32041 ggaggtgtct ctatgacaga gttagaaaaa tgtatggccg gcgaattata taattgtcat
    32101 gatgaaatct ttctgcaata taagaagcag gcaaaagagc tgcttgacat ttataacaga
    32161 ctgacctatg accagaaaga agagaagaaa gggattttac ataagatgtt cggcgggatt
    32221 ggaacaaacg tgtcggtagg ggcgccgttc ctgtgtgatt atgggtgtaa tattattacc
    32281 ggcagcaatg tgtcgattaa tatgaactgc tcctttatcg actgcaatca gataaaaatc
    32341 ggaaataacg tcatgattgc ttccaatgtg cagatttata cggcaacgca tcccgtggaa
    32401 ctggcagaac gcctgacacc agactggacc ccggagagcg gaaagcactt ttgccaggta
    32461 tatgcactgc ccgttgagat cggggacggg tgctggattg gcggtggcgt catcatactc
    32521 cccgggacta aaatcggggc tggcagtgtg attggagcag gcagcgttgt tacccgagat
    32581 atccctccaa attgtgtggc ggtcggaaat ccatgcagag tgattcgcag aattaacggg
    32641 gaaccaaagc atgattaaat tggtggcatt tgacttggat gggacaatcg gagatacgat
    32701 accaatgtgt ataaaggcat tcagacaggc agtatccccc tatgcagggc atttgctgac
    32761 agacagggag gttgttgaaa cctttggatt gaacgagatt ggaatgataa aatccatcgt
    32821 tccagagaaa tggaagcagg cgttatgtga tttctatttt ctatatgaaa aactgcatca
    32881 tgaatgctcc gagccttatg aaggcatccc ggatttgatt gccgctctgc gggcaaaagg
    32941 atttatcctg gcactgataa cgggaaaggg cgaaagaagc tgccggatta ctttgaatga
    33001 gtttgggatg gaacatgatt ttgacgaaat cctgacgggc agtgaacatg aaaatgttaa
    33061 ggcgcagtca ttgaagctgc tgctaaaaaa atatgatttg cttcccgcgc aatgtctata
    33121 tataggggat gcggtttcag atgtgtctga ggccggaaaa gccggggtca tatgcttgtc
    33181 tgctttgtgg ggaacaactg caaaggcaga agaattgaaa agcatcaatg ccgattatat
    33241 ttttgaaacg gttggagaat taaaaaatta cttgatgaaa aatacaaacc atgaaccgga
    33301 ggaggtgtca ataccatgaa aggatttaac cgacaaaatc aattattttc cctttgtggc
    33361 ttgaactgtg ggctttgccc catgtttttg aataaatatt gccccggctg cgggggcggc
    33421 gagggaaatc agtcctgtaa aattgcaaga tgcagcatgg agcataacgg tgtggaatac
    33481 tgctttcagt gtgatgaata cccttgtcca aaatatgagc atattgacga ctatgattct
    33541 tttataacgc atggaaaccg aaaagttgat atgaaaaagg cccatcagat tggaatggaa
    33601 gcctataacg cagagcagga agaaaaggca cgtatcctgg acattctact tgccgactac
    33661 aatgacggga ggaaaaagac gctgttctgt gtggcagtca atctgctgga tctgcaagac
    33721 ctgcaggcgg tactcaggca aattgagaac agagccgatt tggatacact gacgctcaaa
    33781 gaaaaaagcg cttttgccgc aggactgctt caggaagcgg cagcaaagaa caatattgag
    33841 ttgaagttgc gaaagaaaaa gtgaggactt caatactgca attatttaat aaagtattga
    33901 aaaggataga gaatatagat aaatgaacgt aataaacgcc cctttgcaga aaaacaagtg
    33961 atgttctgca aaggaaatca gatattaatt atggaggtat gcttgtaaat ggataaatgg
    34021 ttcacgattg accagattga tgaagataca tatattatca gcgaataccg gcattgggag
    34081 gagacgcatt gctatctgtt aaatggctcc agccgcagct tattgattga tacgggtttg
    34141 ggaatctgca atatttatga tgaagtaatg aaattgacaa ataagcctat tacggccgtg
    34201 gctacccata ttcattggga tcatattggt ggccataaat attttccgga cttttatgcc
    34261 catgcggaag aattgagctg gctgaacggt gagtttccgt tgacaataga aacaatcaga
    34321 gagatggtgg cagaccgctg cgatttacca gacggttacg atacaggcac ttatgagttt
    34381 ttccaaggga cgccaacaaa ggtactggag gatggagaga acattgaatt aggtggacgg
    34441 aatatacagg tattgcatac tcccggccat gctccggggc atatgtgttt ctgggaatca
    34501 gacagaggtt atctgtttac cggagacttg gtttacaagg acactttatt tgcctattat
    34561 ccgtcaacag acccggacgc ataccttgct tctcttgaaa agatttcgga gctaccagtt
    34621 aaaaaggttt ttcctgcgca tcatagtttg gatattcagc cggagatttt aagcagaatg
    34681 agaaatgcgt tcaggcagtt acgggaagat ggtaaattac atcacggcag cggtacattt
    34741 gactatggag attgggcagt atggttataa agagaaggaa gcagtataat ttcatttagt
    34801 tgtgtgctat acaaaaggct ttatcagaac taaagaacag agaaagaggc ttgaaaggag
    34861 catttctatg aagtatgtat gtacagttat atcggtagca gatatcagcg ccgcaagaaa
    34921 gttttatgag gaactgttcg ggctggaggt ataccaggat tacggaaaga atatcgcttt
    34981 tacctgcggt ctggcattac agcaggattt tgactggctg gtaaatatac caaaggaaaa
    35041 agtattgaag aaatccaata atgcagaagt ggtttttgag gagcagaatt tcgatggctt
    35101 cctgaataag ctgaaagaat actcggagat tgagtatctg ggagaagtta tcgaacatag
    35161 ctgggggcaa cgtgtaatcc ggttctacga tctggacgaa catctcatag aggttggaga
    35221 ggatatgcag atggtagtaa agcggttcct tgcttctgga atgactatgg aagaagtatc
    35281 tgcgaaaatg gacgtatcta ttgaggattt gacaaaactt ctgaatagtt aatttgcagc
    35341 gggcatataa acaaattaag atttaccgaa aggagtagaa ctatgaaaat gccagaaaaa
    35401 attgatataa gtatgtttgc tccatgtgga atgaattgta aagtttgtta taagcactgt
    35461 taccataaaa aaccttgtgc tgggtgcttg aatagtgata aagggaaacc tgaacattgt
    35521 cgtaaatgta aaataaagga ctgtatcaaa gaaaaagcgt tatcccattg ttttgaatgt
    35581 gctgaatatc catgtaagtt gattaaagac cttgaaaaaa gctataacaa acggtatcaa
    35641 gcaagtctta tagaaaacag ccgttttatt caggaacaag atttggagca tttcatggaa
    35701 cagcaaaaaa taaaatatac ttgttctaaa tgtggtggaa ttatttccat tcacgataga
    35761 gaatgtagcg aatgtaaaga aaaaatgaaa tagcaaaatc ccagtttgaa aggaaaaaca
    35821 aaatgctact gacatacaga gaagccacta aatcggatgc caatttacta ataaatattt
    35881 ataattcttc attttatgaa gattatattc actatggcga atgtcctggt tatggtaaaa
    35941 caaaggagca aatggaattg tctatcatta aatttcctaa gtatattatc attaaagata
    36001 gtattcctat tggggtgatt tcctttgaaa ataaaggaaa tgggaactat tatcttggct
    36061 gtttatgtgt tattccaatg tatcagagga tggggatagg ttcacaggca ttccaatata
    36121 tgctgtcaat ttgccccgaa tggaagcaaa ttacattggt gaccccttca aacaaagagc
    36181 agaacataaa attctatact gaaaaatgcg gattcaacac tgatggcgaa gagatggacg
    36241 gaaatgtaga agttactaag tttattatga aacgataaat cccagtttat cgaggatatt
    36301 attgtataaa tcaattcagg tctgtcggta agtaacacag cagagatctg gatggacatg
    36361 tcatagcggt tggcgagtat ttagaaaagc tgctgtatag ctgagcggga aagaaagtca
    36421 gaagttgtgg ggtttaaaat tttaagttgc ttttatgcgt tcttggtgga aaaggtgtat
    36481 ttcaattgaa aggatggtcg ggtgctaatg aaagaaatca tcaacttcat tgaagcgaat
    36541 gtggatggca aaacgctgtt tacaaaggaa ttagtttacg aacttgaaaa tggtgtgttg
    36601 cagggcgttt actcagatca aatatctttc tctaatctaa aatattctca gagcggattt
    36661 cagttggata tgtttattgt atctaatgag aagatatggc tcatgggaaa ggatggagaa
    36721 cgggaaaaat tgcgaaaaga tttcagtggt gtatccctgt tccgatttga actggcgaag
    36781 cgaaaaagca cgaatagttt gactggctgt tttcgtttca tttcagcttc agggaaaaat
    36841 gtggcagcag aggctatcgt aagtggaatt tatgatgtgc gtcttgaaaa tgatgtgctg
    36901 aagttatcgg aagatcaggt cttgtatcgg gatcagccga tacaagaagg gaattttaag
    36961 cccgttgcat ttcaatcgga acatcgtttt tatgttaaag ctaataagct acactatgaa
    37021 tacaacggca agtgttttga tgtggacgca aaaaccatgc ggcgtaacga cagctctgat
    37081 acctttcctc cgtttatttc aatcgaaaag taagaaatga cgaatcggtt tggcaggagt
    37141 gcagagatgt tgattgtttt gagtatataa atgttctaaa agatatgcga aatgtgtcac
    37201 aatagaagat gctttggaaa tgctaataga taaatagtaa agggaagttc tcgatgataa
    37261 ataaaaaaat gatagccttt tgcggtacat attgcggagt gtgtgaatgg aaagacaaaa
    37321 tcgggtgtaa gggctgtaag gccaatagag gaattatgtt ttggggtaag tgcgataaag
    37381 caatatgctg tatagaaaag gggcttgaac attgtgggga atgctcagat atgccctgcc
    37441 aaaagctcag agacctgttt gatgatccag agcatggtga tcacggagca agacttcgca
    37501 acctgaaaaa ttggaaagat ggcatctgta cctatgaaaa attaggcaat acagcgcagg
    37561 agaaggcaaa aaatttgaaa acgattgata acaccaatga ctagaaaaga aagataagat
    37621 tatttcaatg gataagtatt agggcgatga cggtacagtt ccacggtgga aattggtaaa
    37681 agtgattgga acaatcatac actgattttt gggttaataa ttaaaatttg tgaatttgtg
    37741 agagtatata ctatgttcaa tgatggaaga agtaaaaagg ttgtttttgt tgcacattgc
    37801 tttttaaatc aaaattccat ttcagatgga acggcgattt atccggcagc aaataaagat
    37861 gttgttgatt ttttcttaaa tgcagatatt ggtattgtcc aaatgccctg tccggagttt
    37921 tgctgtttag gacttgatag gggaaatatt catggagctg atagcccggt agtggtagag
    37981 aatacacgca tacgttcagc aatgcaaaat gacgaatcga atttgaaact aacaagactg
    38041 gctgattatg tagtacaaca tatcatggaa tataaaaaat atggatttga agttatcggt
    38101 attattgggg caaatcgttc tccaaattgt ggtgtcgaaa caacttcgga caatgatgca
    38161 gaaatcaatg gtatgggatt gtttatagat aaaatatttc gccggctttt gcgagaaaac
    38221 atatctattc ccatgatagg cataaaggga acggacaata tacaagaaaa acttcagcag
    38281 ttaatatgag tgagagccga accaaacagc ggttgctacc gtgaatatca aaaaatatga
    38341 ttgatagttt cagaattgga aaaggaggag ttgcatggat aaaaaagaaa cggcaacaaa
    38401 agaagacttg atggcagtcg ttagcctgtt tgaaaatatg ggaatactat attgggtgga
    38461 cggtggctgg ggcgttgatc tattggcggg taaacagaca agggatcata gagatattga
    38521 tatcaacttt gacgctcaat atacagaaag gctattagat atactttcgg agcatggtta
    38581 taaagtagag acggattgga aacctgttag aatagagtta tatagcgatg aatacggtta
    38641 tttggacatc catccatttg ttttgaatga tgacgggacg tcaaaacaag ccgatttgga
    38701 aggcggctgg tacgagtttg agaaggacta ttttggtagt gctgtttttg agggtagaac
    38761 aattccctgt atatctttga aaggacaaaa aatttttcac tcgggttatg agctaaggga
    38821 taaggacaag catgatcttt ctgtccttga aagtatatca gaataaggcg aaatggtgat
    38881 ttgcatggag gaagcaacga gatatattgc gcttttgcgc ggtgttaata taagtggaaa
    38941 aaataaagtt ccgatggctg aactgaagaa aatttttgag gagcttgggt tcggagcggt
    39001 taagacctat ctgaacagtg gcaatgtgat tttttcaagt gatgaagaga atatagggag
    39061 ccttacagac cggattgaaa caacgataaa aaagtggttt ggtctggata ttccgatttt
    39121 cgttatatca aaagaggatc tcgaagatat tttgcaacat gcgccggctt ggtggggcga
    39181 tgaaaataaa gaaatctatg ataatctgat ttttattatg cctccggcta catttgctga
    39241 agtatggggc gagatcggag aacccaggga aggattagaa aagattaaga attataaggc
    39301 agcggtattc tggtctttca gccgaaagga ttatcaaaaa acaaactggt ggagggagac
    39361 ggcaagtgcg aacatcagcg gcaaactgac aataagaacg gcaaacactg tcaggaagat
    39421 tgtcggaatg tagttgtttc tcaaattccg gtttgtgggg tgaaaaatat gcagaacaac
    39481 gaattactga atcatttgga taagctacat acgaccgaat tgggtgtaga aagaattaag
    39541 agaaaccttg ctttggatac cgatcatgtt gttgattggt gcagaaatat gataccttct
    39601 gttgaagcat ccatgtgaag acggcagttt tgatgtcaag ggcggaaaaa taaaagtaca
    39661 aaaagtgtag aaaacaaggg gtttccggga gttgggaatt gccgacgggt agttccggct
    39721 cccggatttt ttacgcttca tagttctgtg ggaactggtc tatggcgaaa attggcgtag
    39781 ggccccgttg acaggttgga tatggggtag gttgtgggga taggattgtt ataggtaggt
    39841 tgaggaaaca agatggacaa tataaggcaa cactataata aaaaatcaaa aaccgcagga
    39901 cccattctat ggtatagaag ccaaaaagct gctgaaagct ggcgaagctt tattagatat
    39961 cacctatcag cttggcttta gtagccaaag tcattttacc aattttttgt atagaaaatt
    40021 agacttgaca agcgaccatc ggtcgctata taatgaaggc cgaacaagaa acgaccgatg
    40081 gtcgctatga ggaggtatca taatatgcca aaaggaatta tgcttacacc agaacaacaa
    40141 gaagagcgcc gagaagaaat aatcagtgtt gctttgcagc tcatagagaa aaatggattc
    40201 caaaaaacat caatgaggga aattgcgatt ttagcaaaca tgggaaagtc cagtctatat
    40261 gattttttca aaacgaaaga cgagattgtt gtatatgcag ttgagaaaga aatagaagaa
    40321 acaattaaaa aggttcatag gattattgcc gatgaatcct cgccggaaca gtgtcttaga
    40381 aaaatcatgc tgaatcatct tggagtacca aagcagtatc gaacggtgct gatgtggtta
    40441 aatacagaat ctgactattt agaagaagat tatcgaaagc ggctgaaaac tgcgcgttac
    40501 acatatcagg atattataaa atctgtaatt gaaaatggag tggaatcagg cgtcttccgt
    40561 aagacggatg ccgatttagt ggcgcgtttg ttaattaact ccattatatc aatcatttac
    40621 acatcccgac catcagccag tccggagaaa atgcttgatg aaacaatgga tatatttcta
    40681 cacggaatta tgaaagatgg aggtgaatta ttatgattta ttatactcta cccttgttgc
    40741 ataaacaggc aacccttgaa atggtcggcg gaaaaggtat gtctttatca aaacttttga
    40801 cagcgggtat ccctgtgccg gagggctttc atgttacgac tgcttcatac aagatttttg
    40861 ttgaaaagaa ccatattcag cctcatatta ataagttgct ggacggtatt gactctaaca
    40921 ataccagcca gcttgaaaat gtatctacgc agatagggat gcttttccat aatggagaaa
    40981 tgccgcagga agtatcagat gcaattaaaa ccgcatatgc cggattggga aatatagcag
    41041 tggctgtccg ttcctccgca accgccgagg atttgcccga cgcttctttt gcaggccagc
    41101 aggaaaccta tcttaacata caaggtgaga atgaagttct tgacgctgta aagaggtgct
    41161 gggtttccct atggacagct cgcgctattg cttaccgcgt gaagaatggt ataaagcagg
    41221 aaattgttgc acttgctgtt gtagtacaaa agcttgcgtt ttccgatgcg tccggcgtta
    41281 tgtttacgct aaaccctatc aatggcagac gtagcgaaat gattgttaac gccgcgtggg
    41341 gacttggcga atcggtggtt tctagcttag tcacccctga tacaatcgtt gtagataaaa
    41401 atgctgaacg aattgtatcg tatgaagcag caaacaaaga gattatgaca gtccgcacct
    41461 cagatggaac agaagaaatc ccaactccta aacagttgag gaaaaaacat gctcttactc
    41521 gcaatcaagt tatgcgattg acacagcttg ggaaaaaaat tgagaagtat tatgaaatgc
    41581 ctatggatgt agagtgggca ctggaaaaag ataagttgta tattgttcag gcccgcccca
    41641 ttactgtcct gccgccggaa tggacgttac cggaaaagga tgttatatat acaaaaggca
    41701 gtctggcgga acacttgcca aatcctgtta cgcccttatt cgccacactt ggacttgaaa
    41761 tagtcaaccg cgcttcggcg cttttatgga tagatatgtt tggtaaaagt gcgaaaaagt
    41821 tactgccaga aaacggagca tatacgataa ttaacggata tgtttatctt tccgctaatt
    41881 caaagccttt tttgattgct gtcaaatccc tttcaccccg atctttacgc cgtgcgctca
    41941 cgaacagtgt tgcacgctgg gaaacagcac gaaaagaatt tgaggatgtg attaaacaat
    42001 gggaagaaaa gcccatgcat atgctgaatg ctcatcaaat aatggagggg attcagactg
    42061 tgttttatgg agcgtgtatt tatttcacga ggattcagct tacattgcca gccgcctcta
    42121 tcagcgaaac attatttaca aaattttttc agggagcggc ccgccgtgct ggtataacag
    42181 acacttccgt tctcttgctt gggtttgata cgattgcgct gcaatcggaa aagaacctgt
    42241 gggatctttc ggaatgggca aagcaaaata acactttagg cttctatctg aaaagcaatc
    42301 ctgcaacaaa gatagcagaa gatttcaagt cctccatttt gcctgcggaa gtttcgcagg
    42361 aggtgtggat agagtggaaa agccgaatta atgcctattt caaagagttt ggctgtactg
    42421 cttatgaatt tgattttgca tatgccacac cacaggagat acttacgcca acctttgaat
    42481 ccataaaagc atttttggag gagaaagggg aaaatccgta ttcgcgccaa atcgcatttg
    42541 aaaagcgcag gaaacaagcc gaaaacgaga ttttgcaaca gataggtggt caccgtaaaa
    42601 agctgttttt gaagctgctc cattgggcac aaaatactgc ccccatgcgt gaaaacgcta
    42661 tttatttgat ggggatgggg cacccgctga ttcgccgtat gttacaagaa atttctgaac
    42721 gtctcttaac tggcggagct atttcacatc tggacgatat ttactggctc acaaaaacag
    42781 aattggaaac gctcataaca cagcttgata aaaatatacc cctatctaat ctgagtgagg
    42841 caatccctgc tcgacaagcg gagcatgata cattccgagg ttatgtgtcg ccttcccagt
    42901 tgccggaaaa aaacaaaaaa gccgtatcac atgcaactca aatacagaaa gatgggaaaa
    42961 ttgtattgac gggcatagga accagcgcag gcgttgttac ggctcccgcc tgtgtactga
    43021 acagcccggc agattttgag aaattccagc cgggaagtat cttgatcgcc gttaccacaa
    43081 ctcctgcatg gacgcagttg tttgcctccg caagtgcgat tgtaacagac attggcggcc
    43141 ctcttagcca ttcaagcatt gttgcccgtg aatatggtat ccccgctgtc atggcgacac
    43201 acacagccac gagaagtatt aaaaatggac aaatgataac ggtggacggc tcggagggga
    43261 cggtgacgat tgatgaataa aaagcctatt ttcgctttaa agcttgctac tttggtgatt
    43321 gcgttaggat atagcttaat tcttccggtc atgccatttt acatggaaaa tctaggtgct
    43381 ggtggtcgtg agcttggatg gctcactgcc gtatatgctc tggcacaaac agtatgcgca
    43441 cctttttggg gtgcactgtc tgaccgaatt ggtagaaagc cgattatcag cattggtatt
    43501 ataggatatt ccatcagtct gttcctgttc ggtttggctt catccttttg gacactattt
    43561 atagctcgca gtttatcggg tatattgtca tctgctacgt ccgttgcatc tttggcatat
    43621 gttggagatt ccagctcgga ggaagaaaga agcaagaata tgagtcaact tggagcttca
    43681 atgagcgttg gcgtaatgat gggaccatta ttgggaggca ttcttgcagg ctattcgctt
    43741 gcgctcccat tttttacagg agctggcatt tcatttattg cctttctgct aattctaaca
    43801 cttttgccag agtcaattga gcgttcagga cagacggaaa aaagaaaacc ctttgatttt
    43861 tcagacctga aaagtataat ttttggctca gcaggaatgg ttttgatact tattttcgtc
    43921 gtgaatttct gtcaaacagg cttgcaagga attacggggc tgtatgtcgt agataagttt
    43981 ggattttcca ctaagcaagt tggtgttatt tggatggctc tagcaggtat tttgattgta
    44041 gtacaaggtt ttttgactgg tcctttagca aaaaaaattg gtgagaagcg actaatactg
    44101 ataggtatgt tttgcaaagc cttagtagcg tttgccatgg tattgacaac tgggtttgtt
    44161 agtgtgttgg tgatttttgg cttatttgcg gtatcttctg ctattacagt acccacatta
    44221 aacgcaaccc tttcaaaaac tgacaatcag cataaaggaa cactgatggg atttgccagt
    44281 accgccggaa atcttagcaa agttattgct cctttattaa ctgggtatct gtatgaaagc
    44341 aatattgaat taccatatat aaccacggga gggatagcct taatcggagt ggttatctgc
    44401 tatatctgga taaaaaatca aaagaacaaa tgatggaaga ggacgtttat atggaggtta
    44461 tacacagtaa ctaatctggt atagaaattc aattgaggct tttctgcttt attaaagtct
    44521 tgctaccact taaacctata atatccttaa ttcattatga taaattccaa ttcaacagtt
    44581 ttgatacata aggaggtaac aatactatgg aatagaataa aaaacgaaag gagataagga
    44641 tcatgaatct taaaaaactt gaatttgaaa agcttataga gcaaatctcc actctttaca
    44701 gagagaagaa atattcacag cttaaaagca ttttagcaga cattaatcca actgatattg
    44761 ctgttctttt tgatgaattt ccaaatgaac agaggatttt gctcttccgt cttcttccta
    44821 aagaagaggc ggctgaaaca ttcgttgaac ttgacggtga aacgcaggaa gctctcatcc
    44881 atgctttttc agatacagag cttcgtgagg ttattgacga gctttacctt gatgatacag
    44941 ttgatattat tgaggaaatg cctgcgacaa ttgtaaaaag aatactcaga aatactgatg
    45001 cgcagacacg aaaatcaatt aacactcttt taaagtatcc tgaggacagc gcaggctcaa
    45061 ttatgacccc tgagtttgtt gatctgaaaa aggataacac tgttgaggat gcctttaaaa
    45121 gaataagacg tacaggtgtt gataaggaaa caatttatac ttgttacgtt actgacagct
    45181 caaggcatct tattggtgta actacagtaa aagaactttt gttacacgat tatggcgata
    45241 agattgaaga gtttatggaa acaaatacaa tttttgtaaa cacgcatgat gataaagaaa
    45301 tagcggcaaa tttccttgat aagtataact tccttgctct tcctgttgtg gatatggaaa
    45361 acaggcttgt gggtatcatt acagtcgatg atgctattga cgtattgcag gaagagaata
    45421 ctgaggatat ccagaagatg cacgctatta tgcctaacga gaaaccttat ctcaaaacag
    45481 gcatatttga aacatggaaa tccagaatta cgtggctttt gtttcttatg ataagtgcaa
    45541 catttacgag tatgatttta aatgcttttg aggacaagct ttcagcactg attgtactga
    45601 cctcgtttgt gcctatgctt acgggaacaa gcggtaattc gggagggcag gcgagtgccg
    45661 ttattatccg tgctctttca cttaaagaaa tagattttaa agatatttct aaagttatat
    45721 ggaaggaatt cagagtaggt gttctgtgcg gaattacact tgctgctgtc aattttgtta
    45781 aaatatggtt tgttgacaga acattgctcg gtatgagtgc tattactatt caggttgatc
    45841 ttgtaatcag tctgacactt ataatcgaaa ttatatttgc caaaatggtt ggatgcgtat
    45901 ttcctattct tgcaaaaaaa atgaagcttg acccggctgt aatatcatct ccgtttatta
    45961 cgacggttat ggatgcgctt tcacttttaa tttatttctg gctggcaaca atgatacttc
    46021 atatataaaa aagcagtttt tcaaacctgg ttttttatag tgcgcccgac ggcacacgct
    46081 tctaaagggt gaaaatcctt agtctgcctt agtagtggga aggacacagc cgaacagcaa
    46141 gggtgtccat cgtgaggtgg aatctgaagg aagctgtaag caaatctctg gtctgacaaa
    46201 cagaaatcac atcaggttgc agttaaggat aaggtggtta tacaaactaa agtccaataa
    46261 ctactcggaa ttagctgtaa taaatgtggc agatatatgg agagaaagaa gtgtgtggta
    46321 ctcggggagg tctcgccagc aggagaaaac agagtaagaa agaggtgttt cagaaaattg
    46381 ctgaaagaag aaatatagca ctgaaactta tcatgaagaa gtgattttca aattccggtt
    46441 tgaggggtga aaaatatgca gaacaacgaa ttactgaatc atttggataa gctacatacg
    46501 accgaattgg gtgtagaaag aatcaagaga aaccttgctt tggataccga tcatgttgtt
    46561 gattggtgca gaaataggat atcttctgtt gaagcatcca tatcgagaaa aggaaagaac
    46621 tggtatataa cggttgacgg gtgtatcatt acggtcaacg cttatagcta tacgatcatc
    46681 acggcgcata aggtacagga acagcaaaga tagcaataaa gaactgacag aaagagcggt
    46741 acaagaatat gacttcacga gaaaagaaaa ataaagtaat tcaacaagtt atcaaaccgg
    46801 cgctcaaaga agcaggtttt cacagccttc ggggaacaca ggctttttcc atccataagg
    46861 aagcctgcac aatcgccgta aatattcaaa gctcccaatt taacagcaat gttactggat
    46921 tttccttttg gctgaatatc gtagtagata cgcctgacct ttcagatgaa caactgcaag
    46981 atgttacttt tttcaaatct tttacagaag cttgcttttt gccgcatcaa ggcaggttac
    47041 acccgctccg tgatatacgg ggttatgtca ttgacggtta taaaaactac aagccgatgg
    47101 atacccccta tgagaagatt caagccatca ttgcaaatga tatgcagcag tatatcattc
    47161 cggggctatc cgagattgac agcatagcag attatcaaga atgcgttgcg ctgtggcaag
    47221 aagaatcaaa aagtcagttt gtacgtctgg tagattactt ttcggaagcg catatgctga
    47281 cattaccgtt tcaaaaagat acttggattg gcaccgtaaa tatgaaagag ctcgttgaga
    47341 agaaaaagac actatgcctt accacagatg aaatcatgca aaataaatca atttattgca
    47401 aagtgcgtga attatcaacg catccgcaag aggacacatg gccattgatt tgcatgacgc
    47461 tctctgcaga ataagatgat ttgaaatgtg cggaacttcc tttgagaagg aggagcaaat
    47521 aatgaagcgt tatggaaatg attcgggatc attgcctccg taacgcttgt tcgtattttt
    47581 atacaggtat aaaagtacga tattcgacag accttaacac aaatagcaac attatgcaag
    47641 aaaaagaaat acgaatacac tataatgata ttattggagt aatttttgcg gaggtgactt
    47701 atggatcatt ccaacagtaa aattgttttt gacgaaaaac tgaatataga ggtattaaca
    47761 ctgacaggaa tgattgaaag tttgcctccg cattttcatg attactatga attaggctat
    47821 atagagagcg ggagccgacg agtaatatgt caaggtaagg aatacaccat aggaaaaaat
    47881 gatctgctcc ttttcaaccc gaaagataat catacttgtt tggaattgaa aaaggaactg
    47941 ttggatttta gatgtttcca catcactact gaacgcatgg aagaattgac attggaatgt
    48001 ttaggatttg ccatatgtcc gtattttgaa ccgcagattg tttatcagag tgatcttatt
    48061 ccgcaaatca cagagttgat tgacttaatt gagaatggtt catattgcga tttacaaaag
    48121 gaagaattgc tttatatgat cattggcgat ttggttgcag attattctca accattggag
    48181 tgcgaacaaa tagaagcttc ggatattgta gaacgtgctt gtgagtatat tgaaaggcat
    48241 tacgcctcaa acatatcttt atgcgattta agtaccttta cgggtttcag caagtaccac
    48301 tttattcgga tgtttacgag agaaaaggga atatctcctt atcgctttat tgaatgtact
    48361 aaaatgactg aagccaaaaa aatgctgaag gccggagaag atttatcaaa tattacctat
    48421 cagcttggtt ttagcagcca aagccacttt accaattttt ttaagaaata cgccatggtt
    48481 actccaaaac aatatagtaa gctttataac gcttaaaatg tattttttgt tgagtataga
    48541 tccatactaa caggagatga tgatatggga aattagccgt gatagaaatc tctgagtcag
    48601 gagataaaat tggagaaaaa ctgcttatgc tgttaaacgt ccagcagcgg caggcattaa
    48661 taaaatacct tcttagtcaa gaaaagaagg ataccgattc agctgatagc ccatgtgaca
    48721 gctttcaaat ccatgatccg caggcggagg tcggtggagt attcacagag atcgagatgg
    48781 gagagctata cttttgtttg gagcagcggc ttgtacgtgt gagaggacaa gtgattgagt
    48841 taacagcaaa agaatttgat attcttgcgc tattgctcac acatcccaaa agggtattta
    48901 cttatgaatt gatcatggag ttggtctgga acgaggatta tactttttat tccaaaaaag
    48961 cggtaagcaa tcacatgagt aacctgcgga agaaactcaa agttacaccg gatgctctgg
    49021 actatattaa aaacattgtt ggtgtcggct ataaatttga agcaccataa aaaacatata
    49081 atttgccatt gttagcagaa tagcggtaat cgtcaagaat ctggataaac tggcccgtca
    49141 gctcgccgtg ccttgacgat tatttgcgat tctgctattg ttgtttcata tacggttttg
    49201 acacataata aagacagatt atcgcaatgt aaggagtaat gcaatgaatg aaaaaatctt
    49261 agtggttgat gatgaaaaag aattggccga cttagttgaa gtatatctga aaaacgatgg
    49321 atataccgtt tataaatttt ataatggcaa ggatgcacta aagtgtattg aatccgtgga
    49381 actggattta gccatattgg atatcatgct tccggatgta gacgggtttc agatctgcca
    49441 gaaaatccgg gaaaagtttt acttccctgt tatcatgctg acagcaaaag tggaggacgg
    49501 ggataaaatc atgggactgt ctgtggcaga tgattatatt acgaagccgt ttaacccgtt
    49561 ggaagtggtt gcgagggtaa aggcgcagct aaggcagtac atgcggtaca agcagcccag
    49621 cttaaagcaa gaggctgaat gcacagaata cgatatcaga gggatgacaa ttagcaagag
    49681 cagccataag tgtatcttgt ttggaaagga gattcagctg acgccaacgg aattttcgat
    49741 tctttggtat ctgtgcgagc gtcaggggac ggttgtttct acggaggaat tatttgaagc
    49801 agtatggggg gaacggtatt ttgacagcaa taataccgtg atggcgcata tcggacgtct
    49861 ccgggaaaaa atgaaggaac cgtcaagaaa tccgaaattt ataaaaaccg tgtggggagt
    49921 gggatatacc attgaaaaat agagataaaa ccagtcatga agatgactat ttacttttta
    49981 aaaacagact gtcccttaaa atactgctga taatggcatg ctccattcta attatagcgg
    50041 cagtgtatct attcgtttta aaagataatt ttgcaaatgt ggtggtagcc attttagata
    50101 gctttatcta tcatgatcgg gatgaggcgg tggctgttta tctgagaacc ttttaggcgt
    50161 atgagatatg gctttttctg atagcggtta tgggtgtgtt ttttatgatc tttcgccgtt
    50221 atctggacag tatctcaaag tattttaagg agatcaaccg ggggattgat accctggtgc
    50281 atgaagatac caatgatatc gccctgcctc cggaactggc ttcgaccgaa agaaaaatca
    50341 attccatacg gcatacactg acgaaacgga aaacggacgc cgagcttgcg gaacagcgga
    50401 aaaacgacct tgttatgtac ctggctcatg acttgaagac tccgctttca tcggttatag
    50461 ggtatttaaa cctgttaagg gatgagaatc aaatctccga ggaactccgg gaaaaatatc
    50521 tgtccatttc actggataaa gctgaacgtc tggaagagct gatcaatgag ttttttgaaa
    50581 ttacgaggtt taatctttca aacatcacgc ttgtgtacag caaaatcaat ctgacgatga
    50641 tgctggaaca gctggggcac gagtttaagc cgatgttggc cgggaaaaat ctcaaatgtg
    50701 aatttgatgt tcagccggac atgatgctgt cctgtgatgc caacaaactg cagagagtgt
    50761 tcgataatgt gctgagaaat gccgtcagct actgttatga gaatactacc attcaggtga
    50821 atgccaggca ggcggaagac tatgtgctca tccaagtcat aaacgaaggg gatacgattc
    50881 ccggggagag attggaaaga atctttgagc agttttaccg cctggatgtg tcacgaagct
    50941 caagcaccgg cggagccggt ctgggacttg cgattgcaag ggagattgtg gaactgcacc
    51001 atggacagat cactgcccgc agcgaaaacg gtatcaccag ttttgaggtt acattgccca
    51061 ccgtaggaaa atcgtaagaa attccgagat aaaccgtgtg ttatccataa aataatgcga
    51121 aaaaataaat cgctccattc tggtattctt tatatcagag ggagcgattt ttttgtatgt
    51181 ggaaaggagt taagagaaac gtggaacgtc aaaataacaa tgaaaaccag tatggaagga
    51241 accgcagaaa agacaaaaga agaaaattgt ttttttacag agcagcatgt gccatgctcg
    51301 gtctgctcat agtctgtgta atttttggag ctgtgtattt tctgagagag agtaaagatc
    51361 ccgctcttcc atccaaagaa aatacaaaga caagcaagga ctattcattt ctggccgacg
    51421 gccagagtga ggaggagtct ccagcttcgg agccagccat atccaaccgg gcgaatgcga
    51481 ttgacctgaa cattatagca gcaaatgcca ttgtggtgaa taaagacacc gatgcgttat
    51541 tgtatcaaaa aaacggcacg gacagaattg cgccggccag tacagccaaa atgattacag
    51601 cgttgaccgt gcttgaatat tgttctccgg aggatgagat gagagtcggt gcggagattg
    51661 aaatgatcca tagtgattcg tcaaccgcat ggcttatgaa aggcgatacc ctgactgtca
    51721 ggcagctcct gattgccctg atgcttccgt ccggcaatga tgcggcctat acccttgcag
    51781 tcaataccgg aaagaccatt gcgggtgata atagcctgtc caatcagcag gccattcaaa
    51841 tattcatgga taaggtaaat gagaaggcca aggcaattgg cgttatagac tcgaattttg
    51901 tggttccgga tgggtatgac gccgaggggc agtataccac ggcctatgat cttgccatca
    51961 ttgcaaaggc atgtttggag gatcccatta tttcagaaat tgtggcgagc aatacgtcat
    52021 atgaaagatg gccgaacgga agggaggtca cttacaacaa ttccaatgag cttcttgatc
    52081 cgaacagtcc ctattatcgt ccggaggtca tcggcctgaa aaccgggacc agcagccttg
    52141 gtggtgcctg tgtcgtctcc gcagcagtga ttgacggaga aacctatatc tgcgtagtca
    52201 tgggctctac gaaagaaagc aggtttcagg acagcgttga tattttagat aaaatcaaag
    52261 cccggtaata ggataaggag gaaacgaatg aataaaaaaa tggatattac tgtttttgga
    52321 tgtgagtcag acgaaatgga ggtttttcaa aagatttctt atgagcttgg tgttacagct
    52381 acactcataa aagattctat atcagaaagc aatgctggat tagctgatgg atgctgctgc
    52441 gtaagcgtaa gccataaagc ggagctgtca gaaccgattc ttcttgcact aaaaaatgca
    52501 ggggtaaagt atatcagtac ccggagcatt ggctttaacc atattgatat acaggcggcc
    52561 gggttgctgg gtatggttgt tggcacggta gaatactcgc cgggaagtgt ggccgattat
    52621 accgtcatgc tgatgcttat gctgatgcgc ggcacaaagt cgattctgcg tgaaacccag
    52681 aggcagaatt attgcctgaa tgacctgcgc ggaaaagaac tgcgggatat gaccgtcggt
    52741 gtgttaggaa ctgggcgaat cggacaggca gtcatggaac gcctgaaggg attcggctgt
    52801 aaggtattgg cgtatgaccg aaatcaaaaa gcaggaacgg actatgtttc gtttcatgaa
    52861 ctgctgaaaa aaagtgatat tgttacactg catattccgt tggcagaaga tacccgccat
    52921 atgattggat gtgaagagct ggaaatgatg aagcaggagg cgcttctgat caatacagga
    52981 cggggcgctt tagtggatac cgcagcattg gtagaagcat taaaaggaca gaaaatcggc
    53041 ggtgccgccc tggatgtttt agagggcgaa gaaggtatct tttaccatga ctgcactcaa
    53101 agaacgatag agcatccttt cctgtctgtc ctgcagggaa tgccgaatgt cattgttacg
    53161 ccgcacacag cctatcatac ggatcgggta ctggtcgata ccgtgagcaa taccatccga
    53221 aattgtctga attttgaaag gagtcttgga aatgtataag cttaaaattg cagtcctgtt
    53281 tggaggctgc tcagaggaac atgatgtttc agtgaaatct gcgatggagg ttgcagcaaa
    53341 tataaacaag gaaaaatacc agccgtttta tattggaatc acaaaatccg gcgcatggaa
    53401 actatgcgat aagccctgcc gggactggga aaactatgcg ggatacccgg ctgtgatttc
    53461 tccggacaga aggacccatg gcctgctgat acaaaaggat ggcggatatg agagccagcc
    53521 tgtagacgtg gtgcttccga tgattcatgg aaaatttggc gaggacggaa ccatacaggg
    53581 tctgcttgag ctgtccggca ttccttatgt gggatgcgac attcaaagtt ctgtaacctg
    53641 tatggataag tcgctcgctt atatggttgt gaaaaatgcg ggaattgagg tacctgggtt
    53701 tcgagttcta caaaaggggg acagcctgaa agcagagaca ctctcgtacc cggtctttgt
    53761 aaaacctgcc cgttccggct cctcttttgg cgtgaataag gtatgccggg cagaggaact
    53821 gcaggcagcg gtcacagagg cgggcaagta tgactgcaaa atattggttg aggaggccgt
    53881 ttccgggagt gaggtaggct gtgccatact gggaaatgaa aatgctctca tggctggcga
    53941 ggtggatcag attgagctga gacacggttt ttttaagatt catcaggaag cacagccgga
    54001 gaaaggttct gaaaatgcag ttatcagagt tccagctgtc ttaccggatg aggtaagaga
    54061 acggattcag aaaacagcaa tgaagattta ccggatactt ggctgccgag gattggctcg
    54121 tattgatctg tttttgcggg aggacggctg cattgtgctg aatgaagtga ataccatgcc
    54181 gggttttact tcctacagcc gttatccccg catgatgaca gcagccggtt ttacgcttac
    54241 tgaaatactg gatcgcttga ttgaactttc acttaggagg taactgccat gaaaaagaac
    54301 tttgcctttt tagatgaaat gattcccgga atccgatggg atgctaaata tgccacatgg
    54361 gacaatttca ccgggaaacc ggtagacgga tacatggtaa accgtgttat gggaacgaag
    54421 gagctgggag ttgctttgcg taaggctcag aagatggcgg agaaactggg atatggtttg
    54481 ctcttatggg acggctatcg cccccagtgc gcagtggatt gttttctgaa ttggacttcc
    54541 caaccggaag acaatctgac gaaaaagcgt tactatccaa atatcaaaag gaatgagatg
    54601 gttgcgaagg ggtatgtggc ctcaaaatcc agccacagcc gtggaagtac ggttgacctt
    54661 acaatttttc atttgaatag cggtatgctt gttcctatgg gtggagattt tgactatatg
    54721 gatgaacggt cacaccatac cgcaagcggt ctgagcgaag aagaatcaaa aaaccggcag
    54781 tgtttgcgtt atatcatgga gagcagcgga tttgaagcct atcgttatga atggtggcat
    54841 tacgtcttgg tggacgagcc atacccggat acatattttg atttttgtat cgcctgatga
    54901 atctgcccag atgcgccgca gcaagctgac ggggtatctg gcgtggaaac ataagctccg
    54961 taatcttttg ccatagatag gttcactttg tctgttacag cggaaacttg acatttgatg
    55021 tcttatttct gtaaaattga cagaggaggt gccttgaatg aaaagcaaca gaatgttcgg
    55081 tatattatgt attttattgg agcggaaaaa aataacggca caggagctgg cggagtattt
    55141 tgaggtctct gtgcgcacga tccataggga tttgttggat ttgtccagtg cagggttccc
    55201 agtgactaca cagcagggca ttggcggcgg tatatctttg ctgccaaatt ttaaatacag
    55261 caaatcagca ctgaataaag aggacataga tctaattgtg tccggcatac aagggtttgc
    55321 aagtattgat gagagttcaa aaataaagac actgcttgcg aagctgcgtc ttgggcagga
    55381 agataagctg ctgctggaga acgatatcat cattgatttc acatcctgga accataagag
    55441 tacgaccatt gaaaaaatca aaatcattcg ttctgcgatt gcctcccggc gcctgttgga
    55501 actggagtat tacagcagca atggatactc caaaagaacc gtggaaccct ataagctcat
    55561 tttcaaagaa gaatactggt atcttttcgc ctactgcaca caaaggcagg atttccgtgt
    55621 ttttaagctt aacagaatat cgaagctgtt gctttgcgag cagatctata cggaacggac
    55681 agattatgaa attccggtat tgcagagcgc attttcaaat ggcgtggggc aattggttac
    55741 agtcagaatg gataaatcct atgagtttct ggcgattgat ttctttggcc agagcaatat
    55801 ccgggaagaa aataattctc tttacatttc cttttatacg gaatatccgg agtggatcgt
    55861 aagcactttt gcaagcttgg gggataaagc ggaaattata gagccaaaga cgctgcggga
    55921 tgatatcaag gcatttttag agcaggccag aaaacaatat gataaatgag aaccagcgat
    55981 tctcactttt acgataggaa atgctttgca cagattggct cataaaaatc ctcttgacaa
    56041 gtaaacgagc gtttactata atatcaaaaa gaagaggtaa acgattgttt acattgtagg
    56101 aggtacatag aatgactgat acaaaggaaa aaatattggc cgttgcattg caattatttg
    56161 caaaagacgg atatgaagca gtttccgtca gtaaaatagc agaaaaattg ggaattacaa
    56221 aaggggcgct gtataagcac tatgaaaata agaaggatat ttttaacagt attgtggcgc
    56281 gtatgtcaca atcggatcat gaaatagcac ggaaatatgg tgtgccagaa aatgtatttg
    56341 attgtgcacc agaacaatat gagaaaatcg gattgaaaaa tcttaaaaat tttgccgttt
    56401 cacaatatag gttttggact tcagatgaat ttgccgctga ttatcgtaag atgctcacat
    56461 tagagcagta tcgtaatcca gaattaaaca agttgtatca ggatagcctt gtgcgtgggc
    56521 ctgttcttta tttaaaagat atttttcgtg agatgattag atgtggtgtg ttaaaggaga
    56581 atgacccata ccaacttgca atagaatttt tctccccgct ttttttactt atcaatatgt
    56641 cagatgaaaa tgaatcttat gtggatattg aaaaacggct aataaagcat attgattttt
    56701 ttatagaatc tcacacaaag gaaagggaaa acaaaaatga aatatattcg tagtcaaaaa
    56761 tataacattc ctgaattaca ggctaaaatt atggggccaa atccagtcaa actggaggag
    56821 gaacttttac ttcattgcaa aatctcccaa aacgccatag tatgtgatct tggcagcggt
    56881 caggggttga cttccctctt tcttgtaaaa gagtacgggt ttactgtata tgccacggat
    56941 ttgtggagcg atccggaaga taatcgaaaa ttctttcgca aaatgggttt gtccgatgag
    57001 cagattattc cggttaaggc agatgccacg gcacttccgt ttgaggaaga atttttcgat
    57061 gcaatcatca gtacggattc ctataattat tttgggcgag atcctaaatt ccttgacgat
    57121 aaattgctcc catatcttaa acatggtggc tatgtttata ttgctattcc cggaatgaaa
    57181 aaagattgtc atagtaatct gccaccagaa ttacttctct catggacacc agatcagctt
    57241 gattatatgc acgatataat gtattgggaa aacattatcc gccactgcaa aggggccagg
    57301 gtaatttcca ttcatgagat ggaaagtaat gaggaagtat ggaacgactg gttaaagcaa
    57361 gaaaacgagt atgcaatcag tgatcgtaaa gctatggagg ctggcggagg taaatatttg
    57421 aattttattt ctattgttct tcaaagatta tagcaataaa atcaagagac ggccgagttt
    57481 gattatgaag aagccggatg ctgccttgat acttcaccgg gaagagtcat gtactttttt
    57541 atgatccgga acagtatttt aagtatataa ggccagtgcg aaaagggtag gggcataaag
    57601 cccaggtaag gtgtcattgc acaaaaaaga tatgaaatat gacagaacgc tgtcatgttt
    57661 atagtttatc atagaatgct gaaggacgga ggggcgtaaa gggaacggag gtgatatctt
    57721 tgggaaatta gctgtactgg aaatatccga gccgggagat aaaatcggag aaaagctgct
    57781 tatgctgttg gacgcaagac agcggcagga gttagtaaac tatcttcttg gcaaagaaaa
    57841 gcagaatggt ggttcagctg atatcctgtg tatccgctcc ggagcttatg agctgttgac
    57901 agagaatgaa ggtatcttca cagagattca tatgggtgat ttgtactttt gcctggagca
    57961 gcggctggta cgtgtggatg gacaagtggt cgaactgacg gcgaaagaat ttgatattct
    58021 tgccctgctg atcacgcatc cccaacgggt gttcacatac gagctgatca tggaactggt
    58081 ctgggatgag gctgctgcct gttactcacg caaagcggta agcaaccata tgagcaatct
    58141 gcggaagaaa ttgaagataa caccggatgg cttggaatat atcaaaaata ttgtcggtgt
    58201 aggttataaa tttgaaatac cataacaaag atcacaagga ttttacttcc cctgtggtct
    58261 tgttttgttt atgctccttt tccaaagaac agatcccatt tgttattgtt ctcataagaa
    58321 ttttgccacc tgccattacc ttattgacag ttgtgactga agttgctata atgtactcgt
    58381 tcaataagta cattatatga cggatggagg gatcctgtgg aaattataat aagcagtaat
    58441 acgagtaaac ccatttatga gcagattacc tcacagataa aagctatgat tatgagcggt
    58501 gaactgcaga cgggcgatcc tattccttct atgcgggcat tggcaaagtc aatccatgta
    58561 agcgtaatta ccgttcaaaa ggcatatgaa gatctacaaa gggatggttt tatcgaaacg
    58621 acagtcgggc gtggaagttt tgtagcggca cagaataaag acttttatca ggaggaacag
    58681 cagcggttag cggaagagca tttacaggag gccgctgata ttggcagaac cagtggaata
    58741 tcgttgggaa aattagttga gcttttaact atgttttatc aagaggagga gtgactatgg
    58801 atacaatctt acaggtggaa aatttaacga aacagtatga aggctttcag ctggaccacg
    58861 tttcgttcga ccttccgaaa ggaacgatta tgggactgat tggcgagaat ggagccggaa
    58921 agagtacaac gatcaatgca attcttgatc tgattaagaa agatgacggt acggttactt
    58981 tttgggggca ggaattatct tcatccaaac agttgaagga ggatatcggc gttgtattcg
    59041 atggagtcaa tttttatgaa acattgacgc ctgcgaaagt ggggaagatc tctggatccg
    59101 cctataaaca atgggatgag tacctgtacc gggattatct gaaacgtttc gggcttccgt
    59161 tggataaaga gatcaaaaca ctttccaaag gcatgaaaat gaaattgtgt atcgctgtgg
    59221 cgctttccca taaaccgaag ctgttgattt tagacgaggc gaccagcggt cttgacccgg
    59281 ttatgcggga tgatattctt gatgtctttt tggatttcgt gcaggatgaa aaccattcca
    59341 tcctgatgtc ctcccatatc accacggatt tggaaaaggt tgccgactat attactttta
    59401 ttcatcaggg aaaagtcctg ttttgcaaga caaaggatga actgcgctac aaatatggaa
    59461 tcatccgctg cggtgcagcc gttttcgatc agattgataa agcggagatc ctcgcttacc
    59521 gcaaagacga ttaccagtgg aatgtgctgg tggctgataa agaaaaggcc aggaaaaaat
    59581 ataaagcggc cgtcgttgat gatgctacga ttgacgatat tctgctgctg tatgtgaaag
    59641 gagagcgggc aaaatgaaaa gccttatatt gaaagattta tataatatcg gacacaatac
    59701 aaaatctatg ctgtttatac ttgcggtgtt tgctgtggct tttattccta cctctggtgt
    59761 gggagcatat attttcatct gcggcatctt atgcagtatg atgatcgtaa ccactttttc
    59821 ttttgacgat aactccaaat gggcgcgata tgcgatgata atgcccattt caaaaagaga
    59881 tttagtgatt ggaaagttta ttgtcttagc aatcttttgt gcgattggaa gtgtattcgg
    59941 cttggctgta ggcttgatcg gcggaggcat agcaggcaaa attacattca gccctgccgc
    60001 tattgtggaa ctgctgtttt taaccttagc ggcaacggta atttccctca tttttggaag
    60061 tatgtcaatc tcgctggtgt ttaaatttgg agccgaaaaa gggcgcgttt tgcttttggt
    60121 atcgttcctc attccagcag caatttgttt tggggcatat aagctgcttg caatactggg
    60181 cgtggagctg acagatcaga aggtgtttat ccttttatgt tgttccccgc ttatcgcgtt
    60241 ggtttggagt tatggaatgt atcagatcag ttatcggatt tttttgaaac aagaattgta
    60301 agataggaga gattgctatg aaaaaatatg agtatgttaa tatcgaatat tcagcaaaag
    60361 gagtgatgtt ttcttatgtt gaaaagcata gggaaatcat agacagttac gcggagaaag
    60421 gctttcgata cgtcggtttt gtccctacgg aagttggggc aaacggctgc atcagaaaga
    60481 ttgacttggt ttttgaaaag tgattatgtt ttcaatcgaa aatatgggcc tggcgtatta
    60541 tcgagcagtt tgaggcgaaa tgattcaacc ataaatttaa aattcacaaa aaataggaca
    60601 atagaaggat acatcgggaa agaaagcaga atctgccctg atacaattta cacaacaaaa
    60661 gcagatggaa gaagtccact gcttaagtca ggtgaattga aggggaggag gtgcagtatc
    60721 cttatagagg gaataaaaat tctccttaca ctgttgtccg tggttgaaca taagtcttac
    60781 aactgaatat tgtttggcgg cctgttcaaa agcgtaaaca acaaatgcac cgatgcctga
    60841 tattatatcg ggtatcggtg ctttcttcat tgctgttcag gatgaaaggc aagccatttt
    60901 gctctatagg tatccaaata aaaccgattc ccatactcat tttggacaaa atgctttttg
    60961 acctgatacc agccccgcaa ttcagtccgc aaatttgagt ctggagagat tggctccagc
    61021 catatccggg taaactgctg gatctcagat aagattagcg gttttccgga tggcagactc
    61081 ggttcggaaa ataactgggg cagcgtaaac agccttttgg catcagcaaa cggaacggtc
    61141 tgtccatttg ggagcaatag agtatgattg ccggcatcaa ccagcatcca tccatactcc
    61201 gcagaccaga ctggttttcc gtgaaagatt ggaagggttt ccggagcaat tggtacagat
    61261 tggaccgaga tcccctgctc tgaccgtccg aggagaaaat ccgttgttac accatagaga
    61321 acagacattt tctcaagtcc gtcaatggaa ggagacttgc gttctccctc ccatgcgctt
    61381 agggttggct gagagatgcc gagtttttcg gcagcctctg tcatctttaa tttgttgatc
    61441 agccgtgcct gtttgaattg tcttggcatc tgttcaccac ctttattcgt ttatttgcca
    61501 ataaccattt ttatctgagc cgattctttc aataatacct ttttccttta agtttttgat
    61561 tctcccaaat acagctttcc ttccgatatt caatttatca gcaagatttt gataggtgta
    61621 agcaggatcc tcagaaagaa gagcaagaat ctctttttct ttgtcactta aatttacagt
    61681 tccgtttaca gttccattta cagttccatt tgcagtttcc cctggtccat atacaatccg
    61741 atcttttggt gcaataaatt tgatcataac cgaatttccg gtaatatcgt attccggcaa
    61801 tggtacacca tcttcacggc aggcatcaca gatcttttct atgccgcgcc cccagctttc
    61861 aataaaaccg gcaagataat atacatgggc aatattaggg ttaaagggct cagacgagtg
    61921 cttttccatg agcttctctg cagaccagtt ttcaggcagt cggccgcagt ttgcaatata
    61981 taatctgtca ttatatacac ttacctgaat cggtatgcca cgggcatagt ccttatggca
    62041 cagggcattc aagagagcct cgcgcagtgc cgcatctggg acgaaataac gctcgatccg
    62101 ctgcattcct tcgtatgtga tctttgcttt catatacttc agatggatag tttcgataat
    62161 ccgatcaatc tggtcaagca gtgaaccatg tatttcatcc tgataccgaa gttcagaatc
    62221 actttcaaaa taaccgattt ttgtaaaagc accaagctgc cactcttcgg gatcgtttgc
    62281 aaaaaggagc atggcagcat ttgttaagta gtcattattc attaagtgca gctttttcat
    62341 cagcgtctct ttttgctcat ctaaagcgct ctcatcaata cgtccctttt tggccgccca
    62401 ctttataaaa cgcttaacaa caccattatc aatatcagag attgtgaagg acggcaaagg
    62461 aaggttgtcc caggttgcgc cctgtttacg gagcaaaaag ctctccagtt ccaatccggt
    62521 taatttctga ttcgtactgc cactgcggta ataataaacg cctttgtatg agatagcaat
    62581 aggatagggc tgtacagaaa tttcaatata ctcggtttcg ccatctgtat gaaggttgac
    62641 aggcacaata atacccatag catcacggat tttattcgga agatcctcaa gcagcttatg
    62701 ggaattagac aggcctacgg tttcgccatt atcgtttttg ccgatgtaaa gcgtgccgcc
    62761 ctgtgcattc gcaaaaccac agatccatgc cagatagtca tctttccaaa tttctttcca
    62821 ttctataatc tgcatctcag gcattgcttt gttcctcgca tttctttata tatttaccgc
    62881 ttttaatgcg tgcatcggct ggcttttctg catcttcagg aataagccat gtgttaccgg
    62941 tcttctgagt tccggggata cgaccctcgc tgcagaggat acctacacgg cgagaagaaa
    63001 gtccccactt tttgccggct tccgttgttg aaataaattt cataatttat cgccccctta
    63061 actttatata ttatattcgg tttgcggaat aatatcaagg gttttgctaa aaatgtcaga
    63121 atgatttccg gctacacgtt ttccttttct tatttcttac ctcattttta tacataaatc
    63181 atatatttat agctattgac tttccgcttg taacaagtta taatatgact ataaaatcaa
    63241 tttagtcata taggaggcat aaggtatgta tgaagaagtt ggaatttctt caaataatgt
    63301 tccgataaca ctttctattt ggtatacaga aagaaggagt cctaccattg tttttttgcc
    63361 aggtactatg tcacaccctc tcatgtatga gaattttcta tgtggattga gtgaaagagg
    63421 atttaatata ataggcgttc attatctttc acacggaaaa agccctaaaa tcaagaaaaa
    63481 ttatacaatg gaagatatgt tgcataatgt atatgacgca acgacttacg gaatagaaaa
    63541 tttcggtgaa aacatagctg ttatgggttc cagtcaaggc gggattttag ctgcaatggc
    63601 ggcaggtaaa gatacaagat taaaagccgt ttttccacat aatatcatgc tgactacgct
    63661 aaaagaaact atgagcctaa caaaatatcc tgcatggaca caccgtttta tggggctgat
    63721 acaatggggc tttcgcgtgg gcggaaagtt gttacctaac tataaagtac cgggagatgc
    63781 ttatttagac tatgacagag tattttatag tgaacaggca aaacaagatt gcttaaacga
    63841 ccctatgctt ttaccttatt atcctttaag atttgtcgcc agtttattca atgctgactt
    63901 atcgtgcctg gagaatgggt ctatacaatg tcctgttatt ttaattactg ccaaaggcga
    63961 tccgcttttt agctttgatt ataccagcaa agtatttgat ttaattcatg cgccgcaaaa
    64021 ggaattactt actttagagc ttaatcgcca catgattttt aatgaggata cagacacgac
    64081 gataaacgca ttggaaccta cattgcataa atatctcgga tagtatattg atcatatgag
    64141 cagtaaatgt tatacttttc gtgacgatac taacaattta gtcataagga ggttatgaga
    64201 attgccacgg tttacagaac aagagaaaga gatcatcagc ggtaaacttt tgatagaggg
    64261 agaaaagctg tttgcattac acggtttgaa aaaagtaacg gttgatgatt tagtggccgc
    64321 cgtaaacata tcaaaggggt ctttctatgc tttttatccc agtaaagaac atctatatgt
    64381 ggaaatcaat tttcgcttac aaaaagagct atttgaaagt atagaaacaa caattaaaaa
    64441 gaaaaaatat aagaaccata gagatttagc aaaggacgct attaagctaa gtttaacggg
    64501 tttgattact tcacctattc tttcgcagat tgacttatct ataatggact atctgcaaag
    64561 aaaattatca ccggatattt ttgaaagcca tatgcacaat gatattcgta tactggagat
    64621 actggaagat ctaggggttg tatttttggt tccgcatacg gttattataa aatccctgta
    64681 ttctgttttg tcctgtttgg aacaattcaa agaggatgaa gaactgaata tgatacaaaa
    64741 ccttttagtg aatgggatta ttcagcaagt agtagcagag taacaatttt gtttggttat
    64801 ttgacaggca ggtagccaaa caaatttttt acattttata tgactatata tctataagcg
    64861 tcataagatt aaaggaggaa aaataatgat tgatgtaaaa aaactgtatt tcagctatac
    64921 tgaaaagccc tttgttgaag atgtgagttt ccatgttgga cgtggagaaa ttttcggctt
    64981 tttaggaccc tctggagctg ggaagtcaac catacaaaaa gtattgactg ggcttaatac
    65041 aaaatataaa ggcagcgtaa aagttgcagg tattgaaata agggaacgta caaatcggtt
    65101 ttatgagaat attggagtgg actttgaatt ttctacctgt tatgagaaat ttacggcacg
    65161 acaaaacctt gcctatttcg cttcgctgta tgaaaagcag ccccggtcta ttgatgaatt
    65221 attgcatatg gtaggcttgg aaaatgacgg agacaaaaag gttgccgact tttccaaagg
    65281 tatgcgttct cgcttgaatt ttatcaaagc gttaatccat gaccccgata tattatttct
    65341 tgatgaacca acaagcggcc ttgaccctac aaataatcgc ttgatgaaag atattatcct
    65401 tgcagagaag aagcgcggaa aaactatcat aatcaccact cacaatatgt atgacgcaac
    65461 ggagctatgc gatcaggtag cttttattgt tgcgggaaaa gtcagcgcgt tagacagccc
    65521 acataattta atcatgtcaa ggggtgctgc aaaaatacaa tatacctatt atgacaacgg
    65581 cgaaaaaacc ggggaatgtc tgcttgatag aaccacagag gataagatgt taaaaaattt
    65641 aatttctgaa aatcgtctgc tttctatcca tagtagtgaa cctacgttaa atgacatttt
    65701 cgttgatatt acggggagga cacttcaatg agattaggac gcttaattcg tggagatata
    65761 catttccaat ggaaatatgg tttttatttt atttacttta tattaacagt tttatatgtg
    65821 tgtgggattg ccgctttgac gggacattgg aaaacggata ttgcttctat catgatatac
    65881 tctgacccgg cagcaatggg attgtttttt atgggagcga ttgtcctctt ggaaaaaagt
    65941 caaaaggtgt taaatgctat ggtagtatcg cctgtaaaag tatcggagta catactttca
    66001 aaaacagttg cgttgattgc tatttctacg atcattgcgt tgatattggg gtttgtttcc
    66061 ggcagcaatc atttgcttgg tatcacagtt ggtacggcat tgacatcagc aatctttact
    66121 atgattggaa ttattgcggc tacaaaaatt tcaaacttga accaatttct cattgtgatt
    66181 atgccgatag aaattgtttg ttttgtacca cctattgttg gtctgtttgt gaagttacca
    66241 tatctgttcc gcttttttcc attcacagct tgcatgaacc ttataactgg aaaaagtgtt
    66301 ttattatcgc ctgatacggt acttgtaatt gctacgttga tgattctcta catggttgct
    66361 cgacatacag tagagcatat gtggaaaagt ttgggaggtg taaagctatg aaaaagatat
    66421 tatccagcac catacagata tttaagcaaa tcaaaagtga ccctatgatg tttgcggctt
    66481 gcttctcccc ttttattatg ggaactttaa ttaagtttgg tattccattt cttgaaagaa
    66541 taacgaagtt ttctttgcaa gcatattatc cgatttttga tttattgctt tctatcatgg
    66601 cgcctgtatt gctttgcttt gcatttgcaa tgattacatt agaggaaatc gacgataaag
    66661 tgtcacggta cttttcgatt acccctcttg gtaagtcggg gtatctcttt acaaggttgg
    66721 gagtaccctc aattatttcg gcggtcattg cttttattgt actgttgttt ttttcattag
    66781 aaaagttgtc cgttggaatg acagtatgct tggcgcttct cggctcagta caggcaatta
    66841 ttgtttcgct tatgattatc acattatcag gcaataaatt agagggtatg gcggttacaa
    66901 aactagctgc acttacattg ctcggaatac ctgccccttt ctttattgat agttactacc
    66961 agttcgcggt tggctttctt ccatcatttt gggtagcgaa agccatgcag agtgaagcgt
    67021 ttctttattt ctctatagga tttgtggtag ctttagtctg gtactacttc cttacaaaac
    67081 gcctgtttcg gaagctggca ggataaggag gcatattatg gaaaaaggta gacttctgca
    67141 aaacgaaaat ataaataagg ctattcaggt ttccaaaatc acactaatca tcaacctggg
    67201 tttatcgcta cttaaatttg ctgccggata tataggaaag tcaagtgcca tgctgtcgga
    67261 cgctgtacat tcggcttctg atgtattaag cacaatcgtt gtgatggttg gaattaaaat
    67321 atcagaaaaa cagcctgata aagaacatcc ttatgggcat gaacgtatgg aatgtgtggc
    67381 ttccattatt ctctctgttg ccttggctat aacgggtgtt ggaataggat attcagggat
    67441 caagaaaatc ttttcaggac agtacaatac gctttctatt cctagtggaa tcgcacttac
    67501 ggcggcagtc ttatccattg ttataaaaga gtggatgtac tggtttacaa gaaatgctgc
    67561 aaagcataca aattctgatg cgcttatggc tgacgcatgg caccaccgct ctgatgcact
    67621 ctcgtcggtt ggttccttaa ttggcatttt aggagcgcgt ttaggctttg ctattctcga
    67681 ttctattgca agcgttgtga tttgcggttg tatcttaaaa gctgctttgg acattttcaa
    67741 agaaagcatt aacaaaatgg tagaccatag ttgtgacaat gttactgaaa caaagattag
    67801 ggaagttgtg cttaagcagc aaggggttga tggcattgat gaattaaaaa ctcgtatgtt
    67861 tggagcaaaa atgtatgtag atattgaaat attagctgat ggtaatttag ctctttatga
    67921 tgctcatgga attgctgagg cagtccatca aacgattgaa aacaactttc cacaatgtaa
    67981 acattgtatg gtacatgtta atgcgaatga acttctctat tctacatagg acagggaaga
    68041 tgatttaaga ctattgaaag ggaaaggagg gaagtaggtt atggaacaat actggtggat
    68101 tatcattgtc gtggttttaa ttgtcgttgg tttacttaaa cttgtaatta tgaagaaata
    68161 taaccaaaag agaaattcta acaatcgttc aaaagatagc gaggacgaca agatgtatta
    68221 aggtttattg aaatttgtta tgttgtagaa tgatgtttta gatcctagaa agtgctttaa
    68281 ggggggttaa ctttatggaa atctttaagt accattctat acaatcaaat acaatttaat
    68341 aaatctttac aaaaaatcga atgcaataca gctttaaagg ctcgtacaaa tcatatgttt
    68401 tgtgtgggct tttttaattt aagaggttgt agattgtcct gccgaatcgt gtaatgaaaa
    68461 taaggaggcg aatggacatg aagaaaaaaa agattatttg catcatgctg attttaatat
    68521 ttactagcgg cattgtgata ggcgccattc agttatataa gcagtaccat gagtattctg
    68581 aaggcgagga ctcctatact gatttggagg actatgtgaa gctgccggag gaaccggagg
    68641 ctccttccgc atccccagtg gatgatgaaa ctgagtctgg gggacgggag tggccggctg
    68701 tagattttgc ttccctgcag gaaatcaatc cggatattgt aggatggatt tatattgaag
    68761 ggacagaaat caattatccg gttgtgcaag gtaacgataa ccagtattac ctgaagcatt
    68821 tgttcagcgg tgagtggaac ggatctggct gcatctttct ggacagcaga aacagagtgg
    68881 atttctcaga ccggcacagc atcatttatg gacatcatat gaaaaatggc agcatgtttt
    68941 ccgggcttac ggaatataag aagcaggagt tttatgattc acattctgtg gctttgctgc
    69001 tgacaccgga taaaaattat gagatagaaa ttttcgccgg atatgtggcc agtgtccagg
    69061 ataaagcctg ggaattggct ttcccctcag acccggattt tacagggtgg ctggataagg
    69121 caaaagaacg ctcgtgcttc accagcggaa ttactccggc agtcacagac cggatgctga
    69181 cgttatctac ctgcagctac gagtttaaca atgccaggtt tgtattgctt ggcatattaa
    69241 agccggaaga gtaacggatt cagaagaaaa tagaataatg gattgaattg gggggatgct
    69301 attacgaaaa aaggtgcgtt tccccgataa ttcgtaatga aaactctctg ctcctatatc
    69361 aagtgcagat tacgtttcat ttccatagtg atctaacctt acgattttta tagaagtgga
    69421 tctgaataat tgcggcaatt tagcagcgat gggcttacag gtggaaagat gattctcaac
    69481 gacttgtaaa agaacgatgg taaaacgatt tccaaagcct ttctgctgac ctaaaatgcg
    69541 taaaaacgca aaaatatcca gtagcatcaa attgtcacca ccacaaacat gtccgttttt
    69601 acaaggggat agtagtagcg tttcaaattg attactgtca tgggctgacc tcctaaaatg
    69661 gcgagaggga caagcagctt gctactgcct gtccctctcg gtgatggaga ttgttcccct
    69721 cacttggtta gcctgaaaat cgtgtttcgt ggggtctgtt tttgaaaaaa tcttgaaact
    69781 tttttctggc cgcctccacg gaatccctca cggtgtactt ggagcagccg tccagccggg
    69841 cgatctgctc atagctcaat ccgtccagcg cgtagagcag gaaccggcgg cgctgtgtgg
    69901 cggtgcagct ctccaacatg gaaagcagtt cgtccagcgt ttccctccgg caaacgtatt
    69961 gctctggcgt ttcttggttg gtgcggtcct cggcggcgac tatgtactcg tcaaactccc
    70021 ttatgtccca atgccgccgc tgctcatggg ataggttttc ctccttgtgg tcggcccggt
    70081 caaggaactc atagacctca acgctgactt ccacggacag ggcttgtccg tttattttga
    70141 tgatgacttc catctgcctt tcctccgttt cgattggggt tggtgcgaat cgaaacgggg
    70201 ggagcggaga gcggcaacag tgccgaacat cgggtcaact tgacccgaat tttaccgtct
    70261 gaaaaagaga ggtttccggc cctcctgcgc cgaatttttt tattttcttg taaactggag
    70321 gcccggtaaa aacaaaggct ctgaatttct gcaaatatcc ccggcactcg tctgcaatcg
    70381 cagacgaacc gcaacggaat agctcatgta ttgtcgcata aaaaagggcg caagtgattg
    70441 gcttttcact tgcgccgtgg tggacgtttt cgctatttta ttgtggcttg tcgcccatgt
    70501 aagaggcgcg tcaaaaggga tatttgctca ttgcgaccct ccactggccg cataaaaaag
    70561 aggccttgat tatttcaaag ccaccgaaac taataaggtg cgtggaaata tagttgctac
    70621 acgacggctt tttttctttg ccgtcgtgcg gcaactattg aagttatttt acatctttta
    70681 ttaattcttc taccgatata ccatatagct ttgctaatgc aaacaagttt gaagtacttg
    70741 ggtctgatgt tccattttcc cattttgata ctgcttgtct gctgacccct attgtttctg
    70801 ccacaaactc ttgtgtcatt tgacaatggg ttcggtacgc ttttaatgtt tctccgagag
    70861 attgactcac ggcagctttc tctttacgaa cattactcga ttttagatat ttcaggaaag
    70921 cacgaacaat gagaacaaac aaagtaacaa acacgccgat aaagattaca ccaaccaaaa
    70981 tagacgttat agaagatttc atatgggtac gcctcctttc ttctttgtta tggctttatt
    71041 ttatcaaaat tgtcgtgttg cagctaccaa ctattacgca attataggtt gcattcgtgt
    71101 tgcggcatat ttttactcaa ataacagcgc ttcgggctga tagaatgcct ttttgtggtt
    71161 ctttcctctg caggctgcta gtgagggcat acacaccatt ggccatgtcc tcggctcgta
    71221 aagctgcttt cttggcctcc atctgccgcc gcttctcggt ctttatcctg tcctcatact
    71281 cgcgttgttt tccattagct ttgcggcgta gatagttctg gtgcagtctg ttcttgcgtt
    71341 cctctttctt ccgtagggcc tcctgttcct ctggggtgag gtttacttct ccgaagtggg
    71401 gaggcacata tcggcccaca aagttaaaat agatttcaac ctcctgcgtg gtttcaatgc
    71461 tgcccttgcg ggcgcgttca tgcacaagga ttttctcgac aaactcgttt aatggcttaa
    71521 tcgctcgtac aatggggtga ttttctcgtt tctcatattc gcgtcatcct tgcatgaaaa
    71581 atgctctaag tgttttcagt aaccatgaga aaaaccatga ttaagaagtc acttagagca
    71641 tatatcacca cttgaaaatc tgaaatagat atgttatact acctacatca gtatcagtaa
    71701 ttgctttcga gcgattagag tttttccggt tgcacagtgt ctgagagtat atactttcag
    71761 gcactttttt tgtttcttct gcttcttttc caagaagcac agaatgggga gtccggaggc
    71821 acaagccctc cgcccatctg ctacaatttc atatcaggcc ctcgggcaat aaaggcactc
    71881 agagaatttc ggttctttgg gtgctttttt tatatagatc ggagctttta cggctcccct
    71941 aatctttgtt ccggggagcc tttcggcata cccgtgtata tagataaaac cacgcaggaa
    72001 tgcagaaagg aaatgcctat gattctttat ttggatgcca gaactaccgt caaggatctg
    72061 ataattgatt atattgaggt ggaattggca aacggagaaa ccgcctctct taattgggat
    72121 gaaagtgata ttgggcgggc tgacgacggt ttcagtgccc gatacaaagg ggtgtatttt
    72181 ggagaggtct atgccaatgg cagactggag cagctgcagg atatgaaaat aaccgatatc
    72241 ggtttgtatt ctgaaagcga tactcctctc aatatctgta tcacttcgat ggaatttgaa
    72301 gacgatggca ggctattggc gtttgaagct ccgattttac atggaaacat tgtttatcag
    72361 aacgaaagtg gtgaggtgat cgcatgttga aaagcggaaa atatatcgcc gaacacgata
    72421 acggaatatt tgaccgatac cggattgtta tgtcagtaaa ggaaacggag aagtcatata
    72481 tttttgaatt gcttgagtat gtaagccggt atagttcggc acagatggac atgctgttcg
    72541 acaagagtaa gcgggtcctg atttcaaaat tcaagggcgg ccatgcaatg cgtatatgga
    72601 gtgaccatga ctttacactg tatccatatc aagccgggat tccgtttcat tttgaacggg
    72661 tcagtgaagg cggcagtgcg gaaggaagtg gcgtctatgg gtaagaaaaa aacgtacaga
    72721 ggcgagctgg ttgatgaaat cagaggtctg gggcagtcac aagggctgaa tcatgtcttc
    72781 actacttttt tggagatttc agcaacctgt atctctgcag agatggatcc gtcaaacgca
    72841 gaagaacgcg agaagcggta tgaggaaatg acatctgcta tggatcctaa aaccctcaac
    72901 ggctatgcgc atatgctcgc tctgctggct cttgctattc atgagcatga ggaggatccc
    72961 tgcgatattc ttggcagtat ctatcatgag cttcgcttaa ataacgagtg gaacggacag
    73021 tatttcactc ccgataatat ttgccggatg atggcgcggc ttgtaaaccc agtggatgag
    73081 cgctctgaaa cggaagcccc tatcatgatc aacgagccta cttgcggttc gggtacgatg
    73141 gtgattggtg cagtatgggc catgaagcag aaagatttcg attttcggca caagagtttt
    73201 tttgttgccc aggatattga catccgctgt gtgtggatgg catatatcca gctcagtctg
    73261 tatggaattc cggcggtggt cattcatggc gacaccttag caatgaaaga atggtcccga
    73321 tggtacactc cgttggcgtc tgttcctttc ataaaaaggg aatgcgctgt caatagcaca
    73381 gagaaagggg cggcgacaag tggatagtac agaaaaatct ttggacgatt tgacctttgc
    73441 tgatctgcga gtccattacg gaaccggacg tgcttttctg atcagacagg agtatagaag
    73501 gaatgtttac ggctaccgaa aaggcgttaa gacagatctg ggagacctgg aggaaaaaga
    73561 ttggatacag ctggccacgg gtttgattca aaaaagcgga gagcaacagc tgcagaaaaa
    73621 tctgttggaa tgggagcagg agcataatta ttgcaatagt tccctcaaag agatggaggt
    73681 gactgcactg gaacttcaca tggccagaat ttttgatgat ccgctctggg tggcctatat
    73741 ccctttcaac cgcaagtatc ggccggaggt tctggaatct gccagactgg tatgggttca
    73801 aacagaatgc tgcgggatac ccgggcaaat tacacaggag cagctggatc agtctgccgg
    73861 aaacgcttta gggattacat gcccgatatg cggccggtgt tccccgtttc aggtctgcac
    73921 gcccaaggag gtgagcggaa atggataagc tgtttatcat tgtaaaaggc ggaagggtgg
    73981 aagaaatcta cagcacaaaa acagaaactg agttgtatgc ggaaattctt gattttgact
    74041 caatggaacg gacggaagag atggaaaaag atcttcagtt ccggtatgag gcgattcggg
    74101 aattgatgta cagaattgac tgaaaggaga atgcacatgg gtgcgaaata taaaacaaac
    74161 catgagcgta tgcttgagct gatcgagcaa ctcacaaagg cagatattgc ctattatcgg
    74221 gatgataatc ccattatgac agaccgggat tacgatatcc tgatggacga attgaaagac
    74281 attgagagcc gaatcgggtt ggttctttcc ggttccccga ctcaaaaggt gtctggagaa
    74341 atattggaaa gcctggccga ggtacgccac agtaaaccta tgctttcagc ggataaaaca
    74401 aaatctgttg aggatctggt gaagtttgct gccaagcagc cggtgctgat aagctggaaa
    74461 atggacggtc tgacgctggt actccgttat gagaacggcc agctggttca ggcgatcacc
    74521 cgcgggcgtg aagggaccat tggtgaggat gtgacacata ctgtgcgcac ttttcttaat
    74581 gtgccgctta ccattcctac gaaagattcg tttgaagtcc gaggtgaggg cgtcatctca
    74641 tgggcaaact ttgagaaaat caattccggt ctggaggagc cctataccca tcccaggaac
    74701 ctggcatccg gcagcacgcg taagctcgac gccggcgaag ccaaaaagcg attgcttgag
    74761 ttttgggcgt ttgagcttgt gagcgattat ctggagtcgg agagtaagct tgcccagcag
    74821 cagtttttgg cacataacgg tttttctgtt gtgccctaca tctatctgga tgcggttcac
    74881 gatgaaacaa tggtgcgtga tgcaatttct gctatggatc cgaagaagtt cccttacccg
    74941 gtagacggaa ttatcatgga atatgatgat gttgcctacg gaaaaggtct gggagccaca
    75001 gggcatcatg aaaaccgttt gattgcattg aagtgggaag atgaactgta tgaaaccaca
    75061 ttcattggct tggatgtggc agtcactcgc actggaatgg tcagtctgac aggcatcttt
    75121 gagccggtgt ctattgacgg cactcaagta agccgggcgt atctgcataa ctatgagaat
    75181 tatctggacc ttgcattggg gcgtggtgat aaaatccgcg tgtataaggc gaacatgatc
    75241 attccgcaga ttgcagagaa cctcaataag accggaacct gctgtgttcc gctcaattgt
    75301 ccctgctgtg gcaagatgct gagtttccgg aaaagcagcg gcggtattcg ccagctgttc
    75361 tgtgaaaatc cgcattgttc tgccaaactg gtgcgaaaat ttgtccactt ttgtgagaaa
    75421 acgaggatga atatcgaggg actgtcggaa aaaactctgg agcagtttgt aagtcatggc
    75481 tggattcgga cgtttgcaga tctttatgcg ctggatcaac accgtgatga gatcgtgcag
    75541 acggaaggct tcggggagaa atcctttgag cgcctgcagg cgtccatcga aaagagccgc
    75601 cactgcacgc tggcgaagtt cattgccggt ctggggatcc ccatggtcgg cagacatgcc
    75661 gggcgtgacc tggacaggca tttccacggc tcgtgggaag cgtttgaaca ggcgattcag
    75721 gacggcttcg actttacgca gctaccggat ttcggcgaga cgatgcacaa caacatttat
    75781 acatggtatg cggatacaga ggaagaaaaa ctctggcgcc cgctgcttga aaaaattgaa
    75841 tttgaaaagg agaattttga tatgaataca aatacagcaa atcccttcta cggcaagacc
    75901 gtagtggcaa ccggcaaact ggagaattac acccgtgacg gcatccagat gaagctgatg
    75961 cagctggggg caaaaccggc aagttcggta acgaaaaaga ccgattatct gattgttggt
    76021 gaaaaggccg gcagtaagct tacgaaagcg cagcagctcg gtatcagaac actgacggag
    76081 gaagaatttg aagcaatgct ggcgtaaatt cgccggaata acagtggcgg ggcagatttt
    76141 ctgctccgct tttcactttt tcatagaaag gaaaagagga tgaatccaaa atatcgctgt
    76201 cctgcttgcg gagcagaaag ctttgaggtg acagctcatg tcacgcagga ctggaagatc
    76261 gactgtaacg gaacctttct ggaatctcta aatgagtgcg tcgaggttac gcattatcct
    76321 gatgagaatg acatttggga ttgtgccaat tgtggcttca gcgcagcggg atgtgagttc
    76381 cgaaatcagt ctgaggagca gaaaggagac aaagaatatg aaccaacaaa aaaagaatct
    76441 tgaaatcact ggcaggctta tctgtccact gtctgttgga actgcagctt ttatcgcaga
    76501 aaatggtggg atacgcagga caagtaatgt tcttcggatg gagaggattt ctcctgatga
    76561 gatccgtttt gagacctgta ataccaacta ccgcctgcat cttatccggc aggaggtgac
    76621 ggcatgagca gtgaaatttt ttatgacaag gcattcatcc ttgtcgggga gaaatatatc
    76681 cccgttgtaa atcatggctc gtcaaactgc tttgactttg atagtcgggg tcgtgagatc
    76741 ccggaaaagc attggtcggt tctgaactac cctcataccg ggaggatgct gtttactgca
    76801 gaagaaatgc aggagatagc agccgtccat gaggaagcca ataggaataa ccggggcggg
    76861 acacgcaaaa gccgaaatcg gtcttttgaa gaaggcgagt ttgggcgctg gatcctggca
    76921 gggatgaaat cggcacatac cgtggaggac tacagaaaac acgggaatac cgtgacagtg
    76981 attgattatg accatgacta ttggcaaagg cactgtgtgt ctacgacaga ggaattgctg
    77041 gataagatca aggagctttc aggccacagc attacagtat ctttctggga cgaccgccat
    77101 gttacccatc cgcccatgcg gcgcaaggga acgccctttg atttcggcac actgccggag
    77161 ttttatgtgc tgcgggcagc gcaggggtat tttgtgaagc ggagcagccg aaagatctgg
    77221 tttgcaaggt ttcaaaagcc gaaatcacag atgattcgga agttcaagac cgaaaaggcg
    77281 gcacaggact atttggacag caatcaaaaa ttcttttcag gatacgcatt tgaaatcgaa
    77341 tgcgtacaga acggtggtgt cacagcatga gggagtcaag gccagaccgc ttccgccgtg
    77401 tggcagaagc ccgggtcaac aagattatca aaatggtaag actgcttgga aattgttcca
    77461 atcttgccgt atatgctttt acgcaagagc aggtacagca gatttttgct acattgcggg
    77521 atgaattgga cagagcgcag aagcgatata cccagtctta caagaaaagg ttttcgctgt
    77581 cggacgaaca gtatgcttcc ctgccgaaat atccgaccat cgtactgcct ctgccggatg
    77641 gaagcagtct tagggcagtt gcaatcgacg atgagaattt tcctgcaatc aatgtttact
    77701 gggatacaga aaagccggag attgatgggc caatctgttt tgcagaatac aatcctgaac
    77761 gcggtccctg ccacgaggtc tgcattggag cttaccagtc tgacaaggaa gacacagttt
    77821 attacaagcc atatatggca gaaagggaat caaatgagta attacatata ctgccgcaca
    77881 ctcaaactgg actggaaaga agtgtccaga ctgatcgcag aatgtgcagg caaaatttta
    77941 aacagaacga ttcatggcac agcgggttat gaggatgatc attactgggg ctttcaggtg
    78001 accactgatc gttttaccat tgccgaaata gacaagctta tccggtttgt taatggggat
    78061 gaggagatgc aacaagaagc aattcctcag gattcggata aatcagccgc aatcggagag
    78121 agtttgtccc gtgctctgtt ggaaaaagct ttaagactgt catggtgcca tgagagtacc
    78181 acggaatcga ctctttggtt ggtcaacatt cgggaaaaac gaccggcagt ctataaacgg
    78241 attgttgaga tcagcccgca tgatatttgc ttggataacc ttaggagcaa aagtgaattg
    78301 attgcttatc tccacgaaaa cggtccaaca cattccacgc tgatggattt ctgcgcggat
    78361 tacagggagc ggtatcacaa cgaactctgc tggaactatc caatctctga tgggctgcat
    78421 cttggcacgt ttttcgttct cgtaaaggag ggcgttctgg ctcttcctta tgacgatgcg
    78481 gataaggtgg actatgagct gctttgtctg gatgatgcca aaatgtgtga cagagaatct
    78541 atggagaatc tcataacgga gtgggatagc ttcgaccggg atttacgctc cgccatgcag
    78601 ggcatgaggg cgttttaccg cagagaggag gagcaacatg aatcagaaaa ttgactggct
    78661 catccatctg gtggccaacg gcgcctgtga tgaatgtggt gaggtagaaa cagacttttt
    78721 gccctatatg tgcaatgctc acacccacgg gctggaacgc tatgggcact tggatttcca
    78781 aatggtactt tttttgccga cggaggagat tggacgaatc ctcaatactt tggggctaag
    78841 agttcagtct ggagaacgct tccggtctgg agatatggtt tccggaattt atgaagactg
    78901 cgatgtgcgc ttggatgagt atgatgaaac cgggcgcaag gtcttgcggg tcatcatacc
    78961 ggatgcgaac aatatcttcc caggggaagg agattgtatg ctcccatact gtctgcagct
    79021 cttgaagaca gatgagctct gcgtagaaag ggggatacca tcatgaaaat cacgttgtat
    79081 caaatcatcc cggaaccgga aaacagatac ttgatattca gcgatttgag aagcatccgg
    79141 gcggcaagcg gcggacatgt tccggctgag atttacgaag cagtattcag cggggatctg
    79201 gacatcgccg tgccccggaa tgccaaaaac aaaatggatc aagaacttgc cgaactggaa
    79261 gcggtatatg tacgttttaa cgtatcgcat ccggaaggct tccgtggacg gtccatgacc
    79321 atgtcggatg tagtcgaggt catccgttcc gaaaaggaaa gcaggttttt cttctgcgac
    79381 cggttcggat ttgaggagat tgcgtttgaa aaggaaaaag ctgattttgg gcgcctgtag
    79441 ggcgcagaaa ggatacaatc atgaagaaac tgatattgaa ctataaaggg cgcgacagct
    79501 gggacagacc ggtttatgaa tcggaaggcc gactgtatgt ggatgtggat ccacgcaaag
    79561 gctggaaacc gaatatctgt acgaagtaca ataatgagtt tgatggagag ccggatacgc
    79621 cgattgccga agataccgtt gtggagtttg ttccgtgtcg ggatatctgg taagcgagga
    79681 ggacgtatga aagaattcat gacaaaaatg gttcctgtca atctgtcaga ccgatttcca
    79741 ttcaaatgca ggatgtgcgg cgcttgctgc cggcatgtca gggaaagtgt gccgctggag
    79801 agtctggatg cttttcggct tgccaaacat ctgagggaca aaggtgaaaa tgtgagctgt
    79861 gtggaagatg tgcttgctgc ctacgcagag cctgtcttac ttagtgaaaa cgggtatacg
    79921 gtatttatgc tgaaaacggt tggagcggat gaagcgtgca tttttctgaa agataatcgg
    79981 tgtacgattc attctgttca tccgcgggcg tgccgtacat atcccattgc tgtcggacca
    80041 tatgaacttg gcgggtatga acagtatctc agtatggagc agccacatca ctattcgggt
    80101 ccacagatgt ctgtgaaaaa atggattcaa aaacgctgta gtaaacagga ttttgaattt
    80161 ttgaacacgg atattggttc agcacaggag atagaaaaat tattgaagaa gattcctgag
    80221 cataaccgga caagggccgt gatgatgttc cttttctaca agtactcgga ttacgatctg
    80281 gataagtctt tccttccaca gtttaaaagc aataacgata agctgctgga ggttctgcgc
    80341 aaaatggcgg atgacaaaga taactaaatt ttcaagtcag tatgacacaa caacaaggcg
    80401 tcgaaattcg gcgccttaat tatttttagg aggaaatacc atggatgaaa gattaaaaaa
    80461 tatcgtagac aattttgagg caatgaaaat cggtgtggat gagccgtttc catttcattg
    80521 caccatgtgc ggcaaatgct gcatcaatcg ggaggatatc cttttgacgc cgcgagatat
    80581 ctataacatg gcaaaggagc ttgggataac gactaaagag ctgtttgaaa cctattgtga
    80641 gacttatatt ggctgcgact cccgtgtgcc tatagtcaga ttgaagcctc gtggctccat
    80701 aaaaagatgc ccgttgatga aagacagaaa atgctctgtc cataaggcaa aaccggcggt
    80761 atgcgctctg tttccgatag gaagatgcct gatgtttgaa aaggattcaa atccttcaga
    80821 gaaaatttca gcaagccaga tacagtatat ttttaccaat cctgggtgcg gtgataatgc
    80881 tgaaacccat acggtgcggg aatggcttgg agagtttgga atgtcactgg aggatgagtt
    80941 ttttgtcaag tggcagcagg caatcatgga attcgggcag tttttccgtg aagcggaaaa
    81001 gacggccagt gagcgtatca tggagctgtc atggacagcg gcttttgtcg gcttatatct
    81061 gcattatgaa actgatcagg aatttctgcc gcagtttgaa aagaatgtcc gggaaatcac
    81121 tgcactgctg cagatggcgc ccataaagga aggcggtaga gcaaatgaat gaggatcgta
    81181 accagcatca ggttatccga aaggatgctc gcaactgctt tgtggagagc ttaaatgatg
    81241 cttttgagat tgggaggatc catcttgctt ttgcgaccta cgatttgagc cgtccgattg
    81301 gacaacggca gacaaacaac atccatattt acattgcggt ggacgagttt ctggaattat
    81361 gccgcaagct ggaaggtggg gaacttcgtt atctgctaaa gaataagaag acaagcgggg
    81421 ataaaacacc gctttatcag tgcctgggtg gtacttctgc cgaaaagctt gcaaaatacg
    81481 ggcgttctcg ggcagatgga aaaagcctgt ccagaacagc acagctattg gcaggaagca
    81541 aatcggattt cctgtttgta gcggatagcg gacctggtga aacagatcag aaaggtttaa
    81601 tcgttcccaa gttcggaagt aagccggaaa atcatgtggc agtcagtatg acatttgaaa
    81661 cattcagtga actgttgctt acgacaaggc tgcactattc agcatggctg ggtgcatggt
    81721 atgcaaacca atatcatccg ggcaaccagc gaacagcaca ggcacaggaa aacccacagt
    81781 atcaggagaa gcagggagaa gcagaatacg cttctgatcc aatgttctaa agtagaatga
    81841 aaaagcggtg cagtctgatg attgcaccgt tttttgcgcc ttgaggggcc catttcaatg
    81901 ggctgaatcc aaaccgactg caaagataca gagccggtaa acttgtgagt tgaaccattt
    81961 gtcggcgttg gcacatttgt gaatgcaaag aaattttatt ataataaacc ctacactata
    82021 acaccatact ttttatatat ggtatgctta acagaacgga ggtggttaca aatgaataca
    82081 tatactactt cggaagtagc aaagatgata ggcatacatc cgaatactgt acggctttat
    82141 gaagaatggg gactgatacc aaatgcagag cgtaaagcaa acggttatcg tatatttacc
    82201 gatttccata ttgaacagtt tcggcttgcc cgtaccgcat ttcaaataga ggtccttcag
    82261 aacggactga gaaaaaagat tgtggaaacg gttaaggttt cagcaaagaa agactttgat
    82321 caagcacttg ttttggcaaa tgaatatctg agtcagatac aaaaggaaga aagaaatgcg
    82381 gaagaagcta ttgctataac aatgcagatt ttagagggga aagcaatatc cgaaacaccg
    82441 ccgcgttgcc tgaaacgtaa agaagtttcg gaatatctga atatttccat ggatacactc
    82501 cgaaattggg aactaaacgg tctgatgcga ataaagcgta agcaaaatgg ctatcgctat
    82561 tacacagacg aagacataaa gcgattaaaa ataataagat cacttcggtg tgccaattat
    82621 tcattggaag caatcctgcg tatgctccat gcgctctcag acgaccctca taccaatata
    82681 aaacgggtac ttaatactcc aaaagacgat acggatatta tatccgtatg cgacagactg
    82741 attatctcat tgcaaaaggc agcggaaaat gccaagaaaa tgataggcat tcttaatgat
    82801 atgaaaagta tattttccta accctccacc tttacaccaa ccttttttga actgctaaaa
    82861 taatgtagta aaaacgaaaa aggaggacag gttgatggaa gaaatcatca gcgtaaaaca
    82921 actttcaaaa tcttatggca gattgaatgc cgtgcagggc ctggatttgt cagtaacaca
    82981 aggttcggtc ttcgggttgc ttggggcaaa cggtgcagga aaaagcacca caatcgaatg
    83041 tatgttaggc acaagaaaac ccgatgcagg tacggtttcc attttaggga tggccccact
    83101 cctgcaaaga aaacagcttt ttgaaaatgt cggtgttcaa ttccaggaag cgaattatca
    83161 agataaagtg acggtttccg aattgtgcga agtaacacag tcgctctata agaatgctgc
    83221 agattatggg gaacttctga aacagtttgg catctttgat aaagcaaaaa gtatggtaaa
    83281 ggagctttca ggaggacagc ggcaaaagct gtttattgtt ttggctttga taccgcagcc
    83341 taaagttgtt tttctggacg agctgaccac ggggcttgac gcaaaagcca gacgagaggt
    83401 atggaaaatc ttatctgacc tcaaagaaaa aggactgacc atttttctga cctcgcattt
    83461 tatggatgag gtagaagcat tatgcgacac aatctgcata ctaaaaaagg ggaaagccgt
    83521 cttttatgga acagtagaag aagccattgc aggcagtcct tatgacaaat ttgaagatgc
    83581 gtacttatgg tattctgacg agggggtaag cgaaaatgag aacatttaaa acaatgctga
    83641 aaaacgaaat gaaactgtcc ctgagggata tgaacatggt catatttgca atctgtattc
    83701 cactcgtggt tttgattata cttggcgtta tttacggcga caaaccagct tttgaaggtg
    83761 cagagtacac atttctcgat cagtcctttg gagctttgtc aacgattgcg atttgtgcag
    83821 gcggcgttat ggggttgcct cttgtggtat ccgattaccg aagcagaaag atattgaaac
    83881 gctttaaggt cactcctgtc agccccgtaa tgattttaac ggtgcaggtg gtgatttaca
    83941 taatttattc cgttgcatcg cttgcattgc tctatatagt atcaacgctc ttttttggct
    84001 tccacctgct tggttctctg cctgctttcc ttggcggata tatattggta atgctctcca
    84061 tattcagcat cggtatgatg gttgggggaa tatctcccaa ctctcagaca gcgagcgtaa
    84121 ttgcaagcct gctctatttc ccaatgctta ttttttctgg agcaaccctg ccgtatgagg
    84181 taatgccgag tgcattgcaa aagatagcgg atattctgcc tttgacacag gggataaaga
    84241 ttttgaaagc tgcttcgtta ggacttccga ttgagaatat ttttgttcct cttattgtca
    84301 tggcggtact tgctgtcatt tgtatcggtg tgtcctttcg ttttttcaga tgggagtaac
    84361 aagtaatatg ggatggctct tattctctct ttgtgggagc gaaaccgtat taggattaga
    84421 gaggacacga tgtctgcaac acgccgagca tgtgaagcgg gccacccttc ccgccgcctt
    84481 cgtcaccctg aattttgacg acggcgggcc gcgccggttc gactacaacg atttcaagga
    84541 gaacgagaaa aggattctcc aacacttcgg ccatttcaag ggcatggagt acgagccgcg
    84601 cagcaaaagc agatcgtggg ctacgccaag acccgcgacg tttacgccgc ctaccgcaag
    84661 gacgggtata gccggaaatt ctatggggag catgaaacgg aaatcctgat tcataaggcc
    84721 gccaaagctg cctttgacga gctgggccta aagaagctcc ccaccatcaa ggccctgcaa
    84781 gcggagtatg ccgaactgct ggccgggaag aaaaaagact atgcggaata cgtcaaggcc
    84841 aagaaggata tgcaggagat tttgacggcc aaggccaacg tggacaggct gttatccacc
    84901 gccccgaccc agccggaaaa agagaaggaa caggcccagc ggtgacgccg ggctgtgtga
    84961 ttttgggaag cgtccgcagg acgcgcagcc gcccgcaggg gcgtttcgac aggggcgcgc
    85021 agcgcacttg tccgggggat tgggggcgag ccaccaacaa gcaaattttt cagttttgag
    85081 cggcagcgaa aaattgaaaa atggccgtta tgttgcactt cgtttccggc ccttcggata
    85141 ggcgtgaaaa aagcggggcc gccgttaggc gtcctccgct tcctcaatcc cgattgcccc
    85201 gtcaatggtc ccctcaatca ccagcaaata ctgtttcggg cataggctca atttatgggc
    85261 cgtccgctgc cgctccgggt cgtcctgctc cccggcctcc gggaaaaagt atcgttccac
    85321 gggcaacttg catatcttca ccagctggta aaaaaccgga aggctgccgc tgttctcaat
    85381 attcgcaagg taacgagggt cgatgttcac cattttggcc aattctctgc gggatatgtt
    85441 cagaacttgc cgggccgcct taatgtcgtc gcccaagctg gcaaagctct ctttctctcg
    85501 ctcttttggc atatcacatc acccctatac attttataat tcatatgata cgccgggagt
    85561 gatgtaatat gcactttaga tacattgaat aatacatttc ccattaaaat gctttacttt
    85621 ttccgtttag gggattgaca ggcgaacaaa tagtagctaa aatagaagga gaacaaacgg
    85681 tagccaaatg gaaaaggagt atatgaacat gttaaaacta tatttcggga acagcattac
    85741 aattataagt acacttttgt taggtgcaaa tattgcctat atgttatggg gatatttagg
    85801 tcggcagaca attcaaaaat ggggcatgat tcttcttctc tttatcttgc tgcatggggc
    85861 attttggtat tttgcaaacg tccgtgatct atattctaac tctattatat ttgccactga
    85921 cggcagcgta gaaatggggt tattttctgt tagcagtatt caatctattg ttttttgggc
    85981 agcaagtgta attgtgtggt tgctcggaat agtatcaatc tttaagctgg aatatagaca
    86041 acatatcttt tacattattg ctatcgtgag ccttgttcaa atagcattta ttgaaggctc
    86101 tcgtatatgg ctttattgct ctgctcctgc tcattttgat tatttgtaag tggggtatcg
    86161 acatggctaa aagaaataca aaagacatca ttttatttga gtccctaaag ctgtttgcag
    86221 ataaaggtta tgatggagta acagtgcgcg atattgcggc agaagttgga attaaacaaa
    86281 gttctttata caaacattat aaaagcaagc aggaaatttt tgatacattg gttgatacaa
    86341 tgcaatcccg ctttagggac gcttccacat cttttcagct gcccaatgga gaattgcggg
    86401 agatggcaaa ggaatacgca acgggtggca acgagatatt aaaaaagatc agtagtgaga
    86461 tttttcactt ttacttaaaa gacccttatg cctctcaatt tcgcaaaatg ctttctattg
    86521 agaaatacaa aaatcaagaa atcgaccgta tctaccgaga agtctatatt gatactgcca
    86581 tttcttatca ggccgcgctt tttgaagaaa tgataaatca aggttttatg cggcaagcag
    86641 acccggaaat aatggcacta caattctttg caccaatttt tcttttatta aataaatatg
    86701 acggcattgt tgaaaaagaa aaagaagcga tagcaatatt gggaaagcat atagaacagt
    86761 tcgacatgat ttatcgtaag gaggcgatct tatgaaagct gcaatcgtat acgctacaaa
    86821 atacggctgc acaaaaaaat gtgctgaaat gctaaagact tatcttaatg gcgaagcaga
    86881 catattatca gcaaaatctg acaaaatcag tttagcgcaa tatgacacgg tttttatcgg
    86941 cggttctgta tatatgggca aaatacaaaa ggaaattacc catttctgca aacgcaattt
    87001 gaagcattta ttacacaaaa agataggtct atttgcgtgt tgttatactc caaaggatac
    87061 agaaggcttt tttgaaacac tttttccact tgagctatta catcatgcgt cctacgtaac
    87121 ctcggttggt ggcaaaatgg attatgataa gatgaatttt ctttacagaa agctcttcca
    87181 gtcgttaaag aaaattgacg gattcaatga aggttttatc gaacctgaaa taaatgcaga
    87241 cgaaatcaag aaattagccg aagttgccaa tatgaattga ggtggtttag tgtgcggaga
    87301 acaatggata atgtgtccta tatgcggtaa caaaacaagg ttgaaaatac gagaagatac
    87361 ggggttgata aacttcccgc tatactgccc aaagtgtaaa caggaaacct taatctgcat
    87421 aaaacaacta catatatcag ttatcaaaga gccagacgct aagacgcaga gccgacaacc
    87481 tatgagctta attctcatgg ttatcggctt tttcttttga taatttgtat cactcgctgg
    87541 caatctttga cggataaagc cgttgtttga cagcgtttgc caacggcggc acaaggagga
    87601 cgtcacatgc cgggagccta taccgtaact ttcgcacaat ctcatagtgt attctcattc
    87661 cgtttccagc cggtaaacag ggtgtcctcc gccgtgctct cctgcgcttg cgggcatggg
    87721 ccgatttctg ctttggcgga gaccggttcc tttcatatcc ccctattcca cgggaaaagg
    87781 ggtgattcca ttgggctact tgaaaagccg cacagaagga ggcggcagca aagcagggaa
    87841 agcccttcta tgataatgac cttgttgtgg cgaaagacaa cggtgcgccc caatcggcaa
    87901 actggatttc ctccggcttc ggcaaactgc tggccgggct ggatatgccg catatccgtt
    87961 atcacgattt gcggcacacg gcggccacga acatgcacca gctgaccggg gacttctata
    88021 cggtcggtga ggtgctgggc cataccctcg ccggtatcgg cgcgtcgctg ggcctctcca
    88081 tgaactttga agccgtcacc acccggtacg tcgatgtgcg tctggaacgg aaacaggaag
    88141 tgctggaagc ctaccacgcc gccgtccacc ccacgctgaa acaggccggg gccaaggaac
    88201 cggggccgga tagagcgaaa ccggcgcaga agaaaaagag ccatgaaatg gagcggtaaa
    88261 cggtataagt tcttaccgct ccgtgaagct cttaacaacc cctcgtggaa tgtgggcact
    88321 atttcacgat ttgacctcga aaatcccaca taaaacgatg gagggaaaga aaaagaaaag
    88381 tcgcacaaac ccagtgttag taaggatttc ataaggatgc tttttagatt tatatgatgt
    88441 aaaaaaccac atacaagggt ataaaagact atcgaatcaa aatgaacttt tttgacaata
    88501 atgagtactt taaaaacggt tcattagata ggttcattaa aacggctgcc tccaagagcc
    88561 gttaaaataa aacctatgat tggcaaaata aaacgacggc ttattaaccg tcgtctaagt
    88621 attttattgc acataaaaac gatacttcag ataacggttt ttttctttac cgctatgcag
    88681 tattgctttc tgtattgacc ttataggatt ttcaccaaat ttcttaaaaa tgttgttgat
    88741 tgaatacttg ttgttttatg agaaatagcg ttctttgttt tcgctgggtg tcaaaaaatc
    88801 taactgcttt tcttttattc ccagactttt tccataatta taggctggtt cccatgttga
    88861 tttgtcagca gttgcatgaa atatcttatt cctataaaca acaatcaact cattctcaca
    88921 ataaaagtga aaataaaact cctgttttac aaatccaatt tggtctacca tatttttttg
    88981 caaacaatta attacggtat ttattttctc ttcatttact tgaatttcat aaatcgtaat
    89041 caatcctaac cagatacttt ttttgtttgt taccgtcaag ttgttaaata tagttttatc
    89101 tttttgactt ttatcaatga tatagcctat atacacttta tttttcatta gaacctccac
    89161 ttagtaattc tatatcattc ttgccagtgc atttgttcat ctgggattcc taatttgcga
    89221 cactcatcac aaacaattga tttctcttta gcatttccaa tttcatattt taatattttg
    89281 tttctaaata caatgaattt agtattttcc tgtttaaaat caacaaacca atttccacca
    89341 cgatttttgt ctgaaatcat tacttttgaa actaattcag ggaaatccgg ttgagcagaa
    89401 gtaaaaaata taactgtcca atatttcggt tttccaccag tattccataa ctcaactttg
    89461 tgtacataaa tataatctaa tatattctca tcctcaatgc tctctttaat taacgcacct
    89521 tcaaataaag ccgatttttt ctttttgatt gcattattcc cacaggaaga aacttcctta
    89581 attccctcaa ctgaaagagt atctttcagt aaaacatcaa cagtaacttt gaatgtattg
    89641 cttaaaataa ttaacttttc agtttctgga agtgcaatat ccgcttccca tttagatatt
    89701 gattgtctgc taacattaca gatttctgct aattcctctt gcgaatatcc atctcgttta
    89761 cgtagtatct gtattttttc tgatagtttc atttagttca cttcctctct tgttaaataa
    89821 ttataacaag tttgatttac aaacactatc aacttttcag ttcttttccg caacctcaag
    89881 gtgcatctcg aatgttatgt gacattttgc aaatttgtca cattctcttc catataggtt
    89941 aaagccttaa aacatcaggg tttcgggaat atagattact gtttcctatt gtatttgtgc
    90001 ccccctgtta gatactgggg gcgaaagcag tcgcggctaa agccactcct gcaaggctag
    90061 aacagacaga caccttgcac tccgtacggt gtctgcccgt tctgcctttc aaattgcttc
    90121 tttttctgct gacgagagta tacttatcag ttcatgcttt atcaagagtg caagcattgc
    90181 tcacgcaaca ggctcttgac aaatcctgct ctgataagta catggtaatc aagcagaaat
    90241 gaatctttat gctgtttttc aacaatagaa aagcacacct gtcttcaata tcagatgtgc
    90301 ttatctacaa ttccatgttc aagttactac aagccttgcc tgtgacagtc tttaaccctt
    90361 tatttttcaa tgtttcttca acaatcctta tgacagccta catttcatgc ggctttccaa
    90421 tgttttatgc gattgtatag agtacgttag accttttgag agtgacgcga aacaacacta
    90481 ttttttatta cttgctttgc agctgtgtca taccctttgt ttcataataa tcggttctcg
    90541 tcataccata taaatagtaa tcgcaatagg tattcaccat tttaccgtcg cggatatgtc
    90601 cctcacgctt aaaccctgct ttttccagtg ttttatatga cgcgatattt agaacatgga
    90661 catcagccca taatcgttgc aattctgttt tttcaaaaca gaaaatcact acttcagtta
    90721 atgcttctgc cataagccca tttccttgat aaatcgggga aagccggaac gccacctttg
    90781 ccatacgatc attttcaatg agatataccc acatctcacc aattacttta ttgtcctttt
    90841 tataaacaat tccccaatga aagctctttg taggccgttc ctttttttga aataacaatt
    90901 ccgggtttaa gtcacttttt ccgggacgtt tcccccaata ttgatacaac gatttgtcgc
    90961 ccaaccattc tttcaaatca tcaatatcat catactgcaa aggacgtaat aggagccttt
    91021 ctgtatcaag tatcggtttg ttctgaatat aatcttgtag tttcataaag cccccttaaa
    91081 aattgaatac ttgttcccaa ttaaagcagc cggacgcttc tgtgcttctc ggccattcgc
    91141 tgcaccatac gggaacgccg ggggtttcca ctctgtcaaa actcggcgta tatggcttta
    91201 tatccaatac gggagtgttg tcgttcgcgt caataaaagt aagccgaata attcccctgt
    91261 taagatcaat actgataatt tctgatgttg ttaaagcaat agggttagga cgggcgggcg
    91321 atcttgtggc gaaaacgccc attactgctg gtgcattttt atagggctgt tcggtttgta
    91381 acaatttacg gtctgcttgg ttatcacaat cgctaaacca ccagattaca ttgatatgac
    91441 taaatccttc caatgcttgc agtccgggaa tatattccgg ctctaattct ataaatgctc
    91501 cgttgtgatc attctttacc tttccaatag gttttaattg ataatcgttc atatcgtacc
    91561 tcctgttttt cgttgtgagt tcagtataat acctcacatc atgtgagata caagagggga
    91621 aataaaaatg ccctgcattt ttgcaggaca ttactacctt aattccaaag gtatttgtat
    91681 ttcagtcagg tactttgccg gattacattc attccatgcc cctctatgca caatttggcg
    91741 cataggattg gacattcgat attcactgtt tttttgcagc cattcagcaa aagcaatata
    91801 cgctcctttg atatttgaaa aatcaccata caccatcgta caggccataa caggtacagg
    91861 ctcaatcgtg cggaaacaga atggagcaat atctttgcat aacttattta caggaacgca
    91921 taactcaata tctacatttt gttccttata ttctgtgtcg tgataaacgg aaaatgtatg
    91981 attggacatt tctattttct gctctttggc aaaagcagaa agttcattcc ataaatcacc
    92041 ctcggagtaa tatgtcggaa caactcttcg caaagataat acttgatact ctggtatcgc
    92101 cttaatggaa atattgtaat gaagttcccc tttgttttct tgtatctcgc tttttgctat
    92161 cgcaattttc cgtattttct cctgctcgtt ttgtattgtc tgctcgattt ctaatcgctt
    92221 tttttcaagg gtttcaagaa gtaaacgatc attcatttca agcgcaagcg caatttcaga
    92281 aacaagaaag ccactgtcac gcaaatacaa tattctgttc agactgggga tttgagttac
    92341 agagtacatt cggtgtcccg tccatttatc gacttctgct ggtttcaaca gccccacttc
    92401 atcataatat cgaagcatac gaatagatac ttgtgttagc tttgaaaatt cacctattct
    92461 aaacatctta tcacctcttg tatatcatta tacttgaagt tttgtatact tcaagcagat
    92521 ttggcacata tccataacgg agaaacggca tagccccaaa agccgcgcct ttttcagcgt
    92581 tgacttgata ggatgtatct ttgtaactgc catatcttcg ccagcgctcc tttgatgtcc
    92641 tcaatgtcct gcgcgtacac aatcccggta ctgttgatac gccgggcaat ctgattgaca
    92701 ttggcgccga tcttttgcag ctcggcggtc tgcgcccgga tgtcgcgggt gtccatgtgg
    92761 acgatatacc cgtcaataag cattttccgg gcataggcgg aaaagttctt tgtgtggagc
    92821 tgggccattt tctgctggat caaggcccgt tcttccggcg tcacagggac acgcagcaca
    92881 aagtttcgtg ttcggtttgc catgttttca ccatcctttc tgaacagggg tttggggctt
    92941 tccccaacaa gcaaccggcc acggcccgca gggacagggt caggccggtt ttcggaagtg
    93001 tggctacact tcctatgatt gcatcttatt aggaacactg taaatacaaa gcttttcaaa
    93061 gcctacaaac cgaaaaaaat aatatttgat ctatcacatt acgcagaaag ccgtccggca
    93121 tgaaaaatcg ggcggctttt ttgcgtggat agaggaaagg aggtaaaaaa taattacaga
    93181 aatttagccc tcaaaattgt gcaactttca cgaaaaggag gatagtgaat ggcagatgaa
    93241 aaattgaaca ccggccccgg cgagcagaac acacccccgg ctcccggccc ggtaacagag
    93301 gaacagcccc aggctcctgc tcccgaacag gccgccgctc cccagccgga gcagtccggg
    93361 cctgcccagc ccgaggcggg ggatgtggtg gtgtcttttg acaaaatcaa tgagctgatg
    93421 gcggaaaagc ggcgtccttg aaggcaagcc ccagggcgtg agagtcaaac cgtgttattt
    93481 ctttgcgttc attcattttc atttcaccct atatcattct acatttctgg tttaattatg
    93541 tgaatgaaac tgaatttcag ttttatgagt attttatttc gtaattttat gtagatacta
    93601 ttgacaatgg ctttgggata ggataaaata aataaaaaca aatagaaaaa ggtgatgaaa
    93661 gtccggcgta tcagatgcca cataatcggt ggtgttgata tgactggaaa agagtagata
    93721 aaatcaattc aataggtaaa aagataactg attgaacagt ttatttttca tgcctaaggt
    93781 ttgattttat tttactctta acttttctta tccttaggaa agattagccg tagagtaaaa
    93841 tctacggctt tttcttttga ccaaacatga aaggagaact tagttatgta tgacaattta
    93901 ctcactattt ttgaaaagcc atctctttac aaaaaaatgg agatcccctt ttgggatgac
    93961 gaacatatat cgttgcaaat gttaaaagcg catttaaatc cagagtttaa tggagcaagt
    94021 agaaaattag attttataaa taaatctgcc gactggataa caagaattgt tcggccatca
    94081 gaatttaatg aactccttga tataggttgt ggtcctggta tatatgctga aaggtttgct
    94141 caatttggtt actcggttac agggattgat ttttcaaagc gctctataaa ttatgcgaca
    94201 agttctgccg ctaaacaaaa ccttaatata cagtatctgt atcaggacta cttgaacatg
    94261 aacttgaaca aaacttttga tttttcaact ttaatttact gtgactatgg tgctttatca
    94321 tcagaagata ggaaaacttt attgaaagtg gtatatacac atctaaggcc cggagggaaa
    94381 tttttgctgg atgtattttc aatgagtaag tacaactgtt ttgaagaaaa gaacacatgg
    94441 aatatatatc cgacaggcgg cttttggaag aaagaaaagt accttgagtt taactcgtct
    94501 tttcggtact cagattgtgt cacattggag caaacatcca tcatttctcc aaatgatcct
    94561 gctgctattt actatatctg gaatacctat tttacaaagg aaactctaat caatgaaatg
    94621 aaatcagaag gttttaaaat atgtgaagta tttagcgatg tagcaggaaa accgtattgt
    94681 gaagatagtc taacgatagc tgtcttgtta gagaagtaaa aaagaaaatt ttatattgca
    94741 gtggcactct gccgaataaa aaaaaccgta agtcggcagg gtgatttgct ataaccggca
    94801 aatagtgttc cgggcaaagt ttcagcttgt ggctgacccg ttgccgctgt tcgctgtccc
    94861 ctcgcatgat ttccgggttg aaataccgtt ctacgggaag cccgcagatt ttaataagct
    94921 ggatcacaac cgggaggctg gggatcgtgc ctttattctc aatattggca agatagcgcc
    94981 attcaatacc caccatttca gccagcgttt tacgcgccag atgttttgct ttccgtgcgg
    95041 cttttacatc cgcgccgaaa gtttcaaaac cgggacaatc ttcaacattc gccatatagc
    95101 atcacccatt tacattgtat aattcatact ttgtccgtgg aatgttgtta tatgcgggtt
    95161 aattaaactt tataattcat atttctttga ttataagaac gcaaaaccat gaaaacaaag
    95221 caaactaatc cgggccctcc tgaacagctc ctaagaaagc gccggacatg tcagcctgtt
    95281 ggccctatac ccatcgtttt cctatgggtt tattctacgc atttcaaagc tcgttccggc
    95341 aggtatttga tatatcattc tgccacccca ccgaccaccg ccgaaaagcc ttgatttctg
    95401 cgagcttttt gtcctctaaa tgcgtagata ggatgagcat ttcagctctc gtgttctgcg
    95461 tactgtttac agtgatgcta cattctataa ttcatattct gttccttgta ttcttcgaga
    95521 aaacgaatta taatgaataa attggaggtg tattatggac tatatgactt tgaaagaagc
    95581 aggtgaaaaa tggggcgtga ctccccgatg gataaattat tactgtgctg catcccgtat
    95641 ccccggagcc ataaaaatgg ggacagtatg gttaatcccg aaagacgccg aaaagccctt
    95701 agatatgcga acgaaacaag gaaaggaatt aaaacatgaa accatataag atactgatta
    95761 ttgaagatga tcctgttata caaagcgaat tggaaatcct tttgcgcggc aatggatata
    95821 ctgctgtttc agtaaaggat ttttcaaagg tgaacgatac ttttagagca gaagatccac
    95881 accttgtctt attagatatt aaattgccca atgaaagcgg cttttctatc tgttctcaaa
    95941 ttcgcagcaa ttcaaatgtt ccaattattt ttgtaacaag ctgcaataca gatatggatg
    96001 aactcaacag catcatgctg ggcggtgacg cctttattac aaaaccctat cacacggcga
    96061 ttcttctggc aaaaatcgca tccctactca aacggtcata cccggtacag cagaatgagc
    96121 aaatggtcta tggtgctacg gtgctacact tggaatccag tagcttggat tatgatggac
    96181 agagtgtgga actaaccaaa aacgaactga aaattcttta ttacctattc aaaaatgcag
    96241 gaaaaatctg tgcccgtaga gatatagtag agtttttatg ggataatcaa ctctatgtgg
    96301 atgacaacgc tctaagcgtc aacatcaacc gtatccgtga aaaattgaca ggtatcggtc
    96361 tgacagattt tatcaaaaca aagcatcgac aggggtacac agtatgaacc gcagacagta
    96421 ttggaaaaat agaatcccat tcctgctgac caacctgatc tgcatggttg cgcttactgt
    96481 attcctgctg gtatgtggca atcctatttc tgtggtacta ctgatttctg cggtatgggc
    96541 gctgatcttg ttggcaggtc tgttccttac ttactggaag cgacagcgac aaatgcgaca
    96601 gcttttagac atgaccggtc agctttcgga acgctatctc gtttccgaag tgatggagtt
    96661 gccggaacag gcagaggatc aggtttacta tcggcttttg aaaatggccg gaaaatctat
    96721 gttggaacag attggaacgg tttgtcagga acgtcaggaa tacaaggaat atattgaaca
    96781 gtggattcac gagatcaaaa cgcctattac cgctatgaaa ctactgtgcg aaaaccatcg
    96841 gacagactgg acaaaggaac tgctgttgga actcgaaaag accaaccgct ttactgaaca
    96901 ggcgctttat tatgcccgca gcgaacatac tgagaaagat tattctgttc gtgaaatggc
    96961 tctggcccaa gtggtgcatc aggcgattgc ggacaacaaa tacctgctgc tccagagcgg
    97021 ggtgcggctg aaagtggagg aaatgcagga tacagtttat tctgatgaaa aatgggtgcg
    97081 gttcattcta aaccaactaa ttgccaatgc ggtcaaatat cgggccgagc agccgattat
    97141 tcgcttttcc gcccgtaggc aacgagatca agtggttctt gtggtggagg ataacggaat
    97201 cggaatttca ctcgctgacc tgcctcgcgt ctttgaaaaa ggatttactg ggcagaacgg
    97261 gcgcatgatc cagcagtcca cagggatcgg cctatacctg tgtaaacgcc tctgtgacaa
    97321 gctgggtatc agcattacag cggcatcatc tgaacatggc gcatctattt ctctggcttt
    97381 tcacattaac tgtctgatcc atgaggtgca gagctaaaat ggttctgcac ttcttacatt
    97441 tctgttagaa ctttgtaaga aaacttgata caaatggcgt tcatattatt ttacaatagg
    97501 attagaggtg ataatactat gaaagaagtt ttgaagctgg aacatatcca gaagtattac
    97561 ggaaatggcg gtaacatcac aaaagcgatt caagacatca gtttttccgt tcaggagggg
    97621 gaatttgtag ggattatggg cgcgtctggc tccggcaaga ccaccctgct taactgcatt
    97681 tccactattg atacggtcag cgcaggacat atccatttgg acgggactga tgtgacagag
    97741 ataaacgaaa agcagattgc ccggtttcgc cgggaaaatc tgggctttgt atttcaggac
    97801 tttaacctgc tggacaccct gaccatttcc gagaacattg ctctagcatt gacaatcaac
    97861 aaggttcccg caggtgagat cgacgggcgg gtacaagaaa tgtccagtaa actaaacatc
    97921 acggatattc tgaaaaaata cccctatcag gtatccggcg gccaaaagca acggtgcgcc
    97981 tgcgccagag ccatcatcaa caatcctaag ctgattctgg cggacgaacc caccggcgca
    98041 ctggacagcc attcgtccca gatgctgcta tccactatcc agagcatcaa cgaaacgctg
    98101 ggtgcaacca tactaatggt aacgcatgac gccttttctg cgagttatgc aaatcgtatt
    98161 ctctttttgc gcgacggcac gatttttacg gaaatccgca aaggcaacga ttctcgcaga
    98221 actttcttta acaagatttt agatgtcctc accatgatgg gagggaattg aaattatgct
    98281 tgcaagtctt tcacaacgta atatgaaacg gcagttaagt gaatataaga ttttttggtt
    98341 ttcccttatc ggcgttgttg cccttatgta tgcgttcaat tctcttattg tttctcatac
    98401 tatgcaacag ctttttcgtt tatttgccga gagcggaaac ggtgatattg gcgtgattgc
    98461 cgcccttttt tctgccgtcg ttgtatttgc acttgggtgg tttatcagct acatgatgga
    98521 cttcatctta cagcgtcgca gcaaggaaat tagcacctat atgatattgg gtgtagaaaa
    98581 aggcgacatt tgcaaaatga tatttaagga aaatgctttt ttgggcctta tggctgttat
    98641 tgtgggcttt ggctttggga ttttagtttc taaaatatta gaaagttttg tttcaaatct
    98701 attccacttt caagaagcat tattagcttt tctttctgta tcagcagtcg gcttgacttg
    98761 catcgaattt tgcattgtct atataatcgc actgattaag acgaatagaa aagtacaaac
    98821 ggttaaattg atagatctgt tgaattacag tcggaacaac agtagtgttc gtgaacacca
    98881 atctaaaact ggcttcatgt ttttgctgat ttccgtggta atgctcattg taagtttcta
    98941 tttgtttgcg ggaacaaaac agacagtggc aagcattacc gcaggtgcag tttcggcaat
    99001 gttggggctg ttgttcttct ttcgaggatt tatttatatc atacacgaaa tgcttaataa
    99061 atctcagaac tggaaataca aaggaagtcg tttagttacc ttacgaatgt tcctttcaaa
    99121 atccaacaaa atgagtttct cattaggtgt tatctcaatt ttattcacct gcaccatcgt
    99181 ttgtattggt atgacaaatg ctttttatca ggtcatggag aaagcagttg ttatgcagcc
    99241 attcgacctt gcgattgttc atgtaggtga gaacggtgat tatagccaat attcctcatt
    99301 cttgaatgaa cgcattgatg tagacaacca atattcctat tgcttatata cagataaaag
    99361 cacacaattt actgacatta gaaatcgtgt cttaacaaaa tactggaaca gggcaagcaa
    99421 aaccgtatca atcaacgact atgttattgc agaaaaccag tacgatgcct ttatgaaata
    99481 tagtgattat tgtaatttgc gagcaatgtt gggcctgcct caaataccat tgtcagaaga
    99541 acaatacatc attcattgcc tgccatatct gaaagataca tttacaaatt ttcagattga
    99601 aaaaggcact ctggtttgca aggatatttt taccgaggcg ttctcacagt atggtggata
    99661 tggtaacggc caagattttg taatcgttgt cccggatcac tatattgaaa atatggaagc
    99721 gatgtacagc ctctatgttg ctcaatccga aacagtgatt gatatgtctg aattggaaaa
    99781 cgaatttccg caagttaggc cattgaactc caatgtggtt gcttccgggg aaaacggata
    99841 cacaacaaaa atcctcgaca aaggaaacta ttactacatg ggcaaattag caagtacacc
    99901 taccagccaa gctatcttaa ttattctgcc attgtgctac ttgtcgctgg ttatcggcat
    99961 aattagtatt gtaatactcg ctgttcaact tttgacggaa gtgaaaacaa taaagcgtca
   100021 atacaatgta atgagaacac ttggaaacga ggtgattgtt cttgaaaaaa tgttgagaga
   100081 tcatatattt ctttacttcg cattgccatt tattcctgca atagtcattg gtagttgctt
   100141 actaaagtca atgagccata cgctttttat agcttcttat gatgtgcctg tatttgataa
   100201 tctaaccgca ttgatagcac ttgtggtttt gagtgcttta ctgattttta cccttattta
   100261 tctcctgtat gcgtttattg cctctcagtc tatgaggaag gaaataatcc ctctaacatt
   100321 agaaaaataa gtagaataga aatttcatct gccggacgac ggcaaaagaa aaaagccgtc
   100381 gtacagcagc cctattcacg cgcccatgaa acggcagctc taaatttgag ccgccgtttc
   100441 cttttgcaga aaacccaacg acgaccctac accttgcaat ttgacatggc ggcaatcagg
   100501 aggacgccgc catgcacgga aaagtttttc gacataaaac aacaaaggcc atcttattaa
   100561 aaaagcctgt tctgcatcgt ttttttgcca caaatagatt ttattttatc tttatctcgt
   100621 ctgtatcact aaccagccat tccattgata catgcaaggc ttttgccagc ataagcaatt
   100681 ctgcatcgca gacatgtcgc tggttcttct ctatacgtga gatgatcaga ggcgtcatat
   100741 cctccatgcc cataaactga atcctttggg caagctcctc ttgtgtaatc gggggctttt
   100801 gcagtgcgcg gcccaatctt actttatcag agcatatatt tccttttatt gtaaacatgc
   100861 ttctacccat tcataaatct ccattttctc tgtcttattt agatattttt tatcgcgtct
   100921 attgatttta tactgaaaca agatataatg ttggataggg tggatattta ttatcttgtt
   100981 tagatagaaa tatgcttttt ttatgattgt ccgcgaccaa tgcggtttat gctttgataa
   101041 tagccatgta gtgagattgc gtagggaaga acttcattgg cgtggtatgg cagataaaat
   101101 ggcacaaaag tggcatagga aatgccgatg acatggaaaa gaaaatctgt tatacttata
   101161 tcgtctacaa gtgggttctc aggaatctgc ttgttttttt attttcagga aaggagttcg
   101221 atactatgaa atactgtcca tattgcggca aagaacttgc aaatccggca gcatcttttt
   101281 gtacggaatg cggggaatcg ttatcttctg cggtaccgga ggaagcgccg gttaccgaag
   101341 cgcagcggga gaagaaaaag agcagcaaga agcatgtaaa gaaaaaaaga tatgcaggaa
   101401 aaacaaaaag accagatgtt cctgcgaaag ctggtggagc atctgagcca gtgagagaag
   101461 atgattatga cggctattat gatgatgtcc gtcccattga tgaaggggga gggcgtgagg
   101521 agattgacaa ggcgctggtt aaaaagatta tattggtggt ggcatgcgtt ctcttaattg
   101581 ccggagcttg tgttgcactt atgtacttga tataaacagc gtaataaaat gttgtaaatc
   101641 accttatcca atcgtgtaat gtaattgaaa ggtcaaagag gtgatgaaca tgatttattt
   101701 gtccaaaggg attgtaaaag aaaactccac ggaacatctt ctccaggtgg cgagatgtgg
   101761 tcaggaatat agcttatccg gagaacaggc ggtgttgtgg ctgaacggga ggtttggatt
   101821 ttccgaagtt aaaacagaat cggagaagcg gacgctgaag catttggcaa gaatgggtct
   101881 ggcagaaacg ggagcagaaa atactgatgt ggcaagatac agaatcctca cacagtgtgt
   101941 ttgctgcccg gcaatcaatg caaaaccaga gatttttttg agccgcgcag aaaaggaaat
   102001 attaatgtgg ctttggaatg ccggacttcg gctgacggta gcagaaatta tctttcttag
   102061 agagcataag atcaggcctg aaccccgata tctccatgct gaaaaccggc aggcgttggt
   102121 ggaggcgatc tacacaaaga ataccatcgc agataattgt ctggagcaga tcatggaatg
   102181 cgcagaatgc agggatgaaa ccataaggat attgctcggc ctgctgaaga aaaagaaact
   102241 aatcgtattg tgaggtgaag cagatgaagg agagatttta tgagttgcca gcacctttac
   102301 aaaagcagat tgccataaga attgtagggg gtgctgtttt cttgctgttg tttgttatca
   102361 tcttgcttgg ttttcgggat ttctatttta gcttaccctg cctgctgttg tccgggtttc
   102421 tgcttataaa cgggattcga ctatgctata atagctttgc tggcaactat atgtgcatcc
   102481 agggaacctg cgagaaaatt gaaacggtcg ggctccgaaa aagaaccaaa gggatctggg
   102541 ttcaactgga gggaaacgta gtgaaaattc ctattcggca aagaatgaag gtgctctcta
   102601 ttggcgatac tgttattata tacttatcag acaaaacccc ggtatatgag catgagggca
   102661 attatatgat atgcagctac tatgcactgg gaatagaaaa gagaggtaat taaaatggaa
   102721 gcagataagg agatgttgaa tcggctcttg aacgaaatac aggagttggt caaggaaaac
   102781 gaaaatgaag acggcaccta ccgatttgac ttgctgaagg cagtcattgc tttggatttc
   102841 gcaaaaacgg agattgaaaa agctattttt aagtgatttt tcagatggat tgactattga
   102901 agctgcatat gctataatgc ttatatcagt ttcaagattt ccaaagagaa atcacgagtt
   102961 tcagagtttt caaagtgtca attagcacaa ttgtgctggt tggcactttt tttgtttctt
   103021 ttggtttctg cctgcaaagc aggtttgaaa taaatatttt ttatagcagg taacccctgc
   103081 gagaaaggag atttccatgt taaaagtttt tgcaacacaa gtcgtcgtat ccaaaggtta
   103141 tgacaacacg ccagctcttc gcttttctga gaatggtgat tctgtgaggt tccgtatcgg
   103201 caagaaggtc tatgatcctc atgctgaaaa cgacagccgc tggatcaacc ttccggtcaa
   103261 agcgtttaac ggcgtatgcg agcgcatcaa gaagatgcag ctgaaagagg gttcctttgt
   103321 gaatctgatt ggcaggctgg acgaagattc ctgggaagat aaggatacca aagaaaccag
   103381 aaaaatgatg gttattattc tggatgacat tgagtatgct tccggtggag gcaagtccaa
   103441 ggaaaaccag gcagctgcag gacagcaaaa tgcggctccc cagaatactg ctccgcaaaa
   103501 tgctgcttcc gttcctgcga caggcgctga agcatccgga aattttaccg ggtatgaagc
   103561 atttggcggc ggatcattct ttgatgagaa ttgattggag caacaattga atcgggcgta
   103621 ttaaggcgca catgagttac agatactgta tgctcatgtg cgcttttttg cgtttcaaag
   103681 gaggtgtctt atgacaaatg aaaccttttg ccttctctct gaggaggaga aacacacaaa
   103741 atttaaggct gcaatgaagg gcaagacaaa aggatgtctg aatgaagaag aacttcatag
   103801 catcctgatg agcatttccc tgaacagaca gtatgctatt tcggtttacc gagacgggga
   103861 agagttttgc cgcgttttga cagcgacgaa tggtctcaaa agaagaacca ttcttggtca
   103921 tgcagccgca gatattggag aaatacccgg aaaacaattt gactcatcgc aggcggcggc
   103981 agtaattttg catgatgctg ttgactgcag cgatgtaaac tgcctctata tctttattcc
   104041 aaaggaacga agtcagaaag gaacagagtg atgaataaga aagatcaaaa aaggcacatg
   104101 gcatatgagg cgctggtact gttgggtatg ctggctttgc ttacttttat atgccggcta
   104161 tggcccatcc tgttattgat tattctcggt atttttgcag cggtgataag actgcttttt
   104221 ctttcatcca gaaagattga agtaattgta ccacttcctc ttttaccaga accggtaaaa
   104281 gaaacgaccg agaaagatgt acaggtgctt gcatattcag tcatcctcag acgggttaca
   104341 gagctggtgc tatctgagta tccggaagca agatgggtct gggaggcacc gaatgcagaa
   104401 aaacttattc aggaaagtca ggaggttttt attttactca atcgtgccgg tggttatcgg
   104461 cgtgccaagg ttgtgatccg aaatctgcag gtggttggaa ttgagtacaa tccacaggtt
   104521 gaggagaatg cggttttgga gccggaggat aatgccccgg aacctgaaga agaaccagag
   104581 gtacaaaatt acgaactgct ggcttttgag tgggcagatg ctcatatttt tgagctgaat
   104641 gccagatgca atgaagcaat tggggaaaat ctctcagaat tgattttgct cgcagaagaa
   104701 cttccggtcc gggagagttg ggcagatatc tgccgggaac tgataagagc cgggcttacg
   104761 gatgttcagt gtgtatctga gggaatcaaa attaatctta cgcagtcaaa tgcagaaagg
   104821 aaataacgta tgagtgcata catgaacaac atctttaatt acagccgtcc cttaccggaa
   104881 ccgtttgata cactgacgaa caaaaaggtg tcagtatcat cgaaatatgg agatgggaca
   104941 aatgcaactt tgtgttcaac agtcataaaa gcggtacatg cagtttgccg ctgcatggat
   105001 ggcagtgccg aaggagctgt cggggtcatc gaccacagga cagttgctga atacaaatcg
   105061 tccatggggc ctgatgagta tcatctggta gtatatgaca gcaactccgg ttcgttaatg
   105121 gcaagcgtgt atgacaagaa cacggaaact tttgaaaact atgtgctgaa cgcatcaggg
   105181 cgtgatggag ctgccgttat gatggcgctg tttccggtac ttatgaatga cgaggaattc
   105241 agtgataatt ttgaattgta ccgcgatcag ttcagtcatg gattttcgga tttacgctcg
   105301 gctacggagt acatggcaat gctatgtgac aacgcctaca gacgtatcaa agatacctcc
   105361 tgttcagcag ccgtcaaggt gagtgtggac aaggccggaa atctgatgcg tgtatctcag
   105421 gtgcagctgg attccggcgc ttttgagcct acccatgtga ttgcggggga atttacgatt
   105481 tttgcaaaaa cagcgagggt tattgtgaag agcgcagatg ccattgtgga acacacggat
   105541 tttgtcggca agtacgaact gcacccaagg actatgagca gtcaggaaaa acagctgata
   105601 ccggtgcttc cggaatggta catcattcca caggaggtcg ttgatatctg taaacatgct
   105661 caggcaacca ccgggaaacc gacacagatg aggaattttc tccttagagg gccggccgga
   105721 accggaaaga ccatgagcgc aaaggcgatt gccgcaggac tggggcttcc gtatatggca
   105781 tacacttgct cggcaggaac agaaatattt gacttcatcg gtcagatatt cccggattcc
   105841 gattcgggtt ctaccggtga tgcacagctt gaccatgaga aagcaatttt ggcgagtatg
   105901 ggcggaatca attatgccaa cgtgtctaaa atgatgaacc ttcccgattt ggatgatatg
   105961 gactacgatc cggcgggggt atatcaggca ctgacgggcg tagagaatgc ggctgccaca
   106021 tctcaggatt gcatgagcat tgtgctggat cgtgtaacag agaaggtacg tgctctgtcc
   106081 agacgggatg aaaattccaa gggcggcggt cagacttata cctacacaga gacagacttt
   106141 atcaaagctc tgaagaacgg atatgtcatc gagattcagg aaccttccac gattgttcag
   106201 cctggtgttc tggtgggact aaactccctg ctggagcaga ccggaacaat caccctccct
   106261 accggtgaaa tcatcaagcg tcatccggat gcagtcgtag tagtaacaac caacattgaa
   106321 tacgaagggt gccgaggcat gaaccagtcc gttatcgaca gaatgagtct ggtgcgggac
   106381 gtggagctgc cgacgcctga agttatggtg cagcgtgcca tgtcagttac aggtgcaaca
   106441 gatgagtacc agatttccca gatggtgcag gtggtcaatg atctgtcgga gtattgccga
   106501 aaaaacagta ttacagacgg ttcatatggt atgcgcagtc tgatcgactg gatcgtaagc
   106561 agtgagatta ccggcgatgt atatgagtcg gcgctatata cgatcatcag caaggcgacc
   106621 acggatgaag tagaccgtga ggcactggtc agtgcagttc tggaaccgat tttcacaaag
   106681 aagcggaaga aagcaaccgc ataaatgtag tagaaaggag gtcaaccctg tatggcgagg
   106741 gtgaatcaca agttagtaaa acagcggctc aatgagcagc gcagtaaaat ttcagaccga
   106801 caattcttct cgtcccgtct tttggcggga cattttgaag atctggcggc ggcacagaca
   106861 aggcggtatc gctataaccg ccgggttcgt gtcaatctgt attggaaacc gagagaaaag
   106921 catgtggcgt caacggacaa tatgtttgtg cgcatcaata ctggaaattc tctggttaca
   106981 aaggtaaaag ggcgagaaaa ccgttaccag attgtgtgcg gtttgtttgc acacgaactg
   107041 ggacatgtcc tttatacaga ctttctggca gcacagacac ataccaacta tttaggaagc
   107101 ggtcgttggt atccctatcc accagacctg aagacaacgg cggatgcacg gaacgagaaa
   107161 gcattctgga attatgtgaa aactgatccg aaaaatctgg agatggtgca gatgattgcc
   107221 catcatatct ccaatgtaat tgaggatggg tatatcgaaa acaggatgct gaacaatttt
   107281 ccaggcacat tggggtacgg tcttgaaaag ctgcgggagc agcattttga gcatatcgaa
   107341 acggtgacgc agatgattga gagtgaagat gaaggaaagc atatttttga aagcattctt
   107401 cagatcatgc tctcgtatgc taagtttggt gaaatcaaat acggggacga accgctcagc
   107461 gatgaaagaa ttcaggcggt gtttggtttg attaccgata ttgacagcgc tttactgagc
   107521 aggtccggaa aagaccggtt gaatgttgtc aatatggtgc tggtaaggtg ctgggactat
   107581 atcgaatctt tttgtgaaga atgcaaaaag cgtcaggaag aggcagtagc ctccggcggc
   107641 tccgccagtc ttgcagaaac cttgtcggaa gtgttgggtg caattgccgg tggttccgaa
   107701 atgggtgaag gaagtagtac accggtgccg gaagcctctg gcgggtcaga agaatctgcg
   107761 acggccggca aacgggcaca gacccatgca gatgccgaca gtgaagatga ctctgaggaa
   107821 acagacagct cccagactga ctctgaaacg gaagaaaatc catctgagtc tgggggaagc
   107881 gataattcta cagacgaaga cgctgccccg atagatggtg gcagttccgg ttcagggaaa
   107941 caagaaactt cagatactga gcagggacgt attccttatc accagtcaga atcactttca
   108001 gaacctgttg gtggttccgt ggaaaaaaac gaagattatg agcgggagca ttacgacagt
   108061 gcggctgccg atattgaacg tcttctggat aagatggcag aaaaggcggc ctgtgagcaa
   108121 ctggagaatg aaaggattca ggagcttaac gatgtcgctc agaacatttc ctatgggaat
   108181 atccatgaag gagtacctat ccgaatcaac cggattgcct ctgtggatga ggagcttatg
   108241 gaacagtacg atgctatttc aggacctttg ctgaatattt cccgccagct gcagaagagt
   108301 ttgctcaagc agctcaagga aaatcggaga ggcggtaaac agaccgggct tatcatgggc
   108361 cgccgtctgg atgctcacgc actgtgcagg aatgacggca aggtgtttta taagaacaat
   108421 ctgcccaatg agatcccgga actggcagtc ggcctgcttc tggatgagtc agggtccatg
   108481 tgttcctgtg accgctgtac ttatgccaga gcttcagcta tcattctgta tgatttctgc
   108541 cagagcctgg atatcccggt tatggtctat ggccattcca ctgattatac cgatgtcgga
   108601 aatacagtag cattgtattc ctatgcggaa tttgatggct ttgatcatga tgataaatac
   108661 cgcatgatgg atattgcggc cagaggcagt aaccgtgacg gggcggcact ccgttttgtt
   108721 gcggagcagc tgtctaagcg gccagaagca gtaaaaatcc tcatactggt ttctgacgga
   108781 cagcctgctg acagtgggta tggaggttcc gcagctgagg aggaccttcg cggcatcaag
   108841 caggagtatc agcgtaaagg tattttgttt gtcgctgcgg caatcggaga tgacaagcaa
   108901 aacattgaga gaatctacgg agactcattc ctggatatct cggatttaaa ccaactgccc
   108961 ataaaactaa ccgctgttgt gaagcggcat gtgagggtat aacaatatca aatcataaaa
   109021 aagacgacgg gcagctggat ttctacagct gcccgcctgt tattcagaaa ggaaggtaat
   109081 tatgcacttt ttaacattag cagctttaga aatctctcag atacaggaag acaaggaatt
   109141 ggacaaccag attgcagagg cgctgaagga attggagctt cagaagcaaa ttgaaacaaa
   109201 gaattttatg ctggattttg cgattgggag atgtcaaaat cttcagtcat cgttctcccg
   109261 tgcagtgaat gacggcgttt ctgagctgat gtatccttat tgtgagtcgc tggaggatcc
   109321 ggaatatctg gaatttgaag atcggacaga aaaactgcgc gaggaatatg aggatgcggt
   109381 tgattgcata aagctgcctc aaggaacagt tgtggagcag tatggcgatc cactctgggg
   109441 cagatttgtt gtccgtgacg gaaaagtgtt ccaaagggat gcaggatcgc ttcatcatga
   109501 aaagcgtaca aagaaagcaa aacgaatggt ggctctgcct aattatcccc gcaaaaagct
   109561 ctataagagt tttgagaaat atgcagaaga aaggtgtggc ttttcttttg atgaaaagca
   109621 tcaaggctat ggatattatt ataatccaaa cgccatatgg gattggtatt ccattggcgg
   109681 gcgatggccg gagatgttcc tggtaaagga cgactgcact gagtattcta tcggtgagcg
   109741 aagctggtgc aactcggacc ggaaatcaga ggcgccggaa ggataccggt gggtatgcgc
   109801 tgccaggaaa aaggacattg cgtgggatgc catgcgggac tggcgaaacc agaaagccgc
   109861 agagcggttc cataagcttg aacagatgtt cctggcagga aagacggatc cggatttcca
   109921 tggagagatt gtcccggacg gagttatgca ctggggcgaa ctggtttacc gcaagggctc
   109981 taccttggag gaatacctgg aggaatatgg gattccgggc agctggaagt atccggtagg
   110041 ctcgcatgac atcgtggatg aggaccagtg gctgagcaaa gatgacagtg tgctggatgc
   110101 agaatctgaa aaatatgttc cagtagactg gcgcagctgc atagatggct acattgatga
   110161 tgtggatgag gatacggtac tggttgccgt agattatcat atgtgaggag gattcagatg
   110221 aatacatatg ttatttgtat ggactctgta tgggttcgtg actcagaaat gtttgatatt
   110281 gtcggtctga cagatgaaga attgacggat attgatatgt gcggcacaga taacgaaggc
   110341 agatggcacg atatggagcc gaccccattt atcgcagtca taaaggcgga aagcgaggag
   110401 gaagcctgca aaaaggcggc cacccaaatg cgctatgatc cacgctgtct gtttgcaata
   110461 aaagtttcag aataaaggag gtatcaaaaa atgattatca atcgaatcgg tgctgagttt
   110521 gaatacgacg gcacaactta tgtaatcggc gctcccatcg tgggaacgcc ggaaagcgag
   110581 tatgagggtc tgtatgggac gattacggaa atccgtgatg gagaggataa ggaaacggaa
   110641 aatgaaacgc cggatatata ttgctctttt gaagttcctg cactgccctg tgaagtaaaa
   110701 aagctggaag aggttttctc tgaactgtat gaccagaaaa agaccattga cgacatcatc
   110761 ctggatcttg tcatcatggc tccgtctatg gtggagccgc ttgatgacct gaaagagtgt
   110821 cgccagcatc caaggattta tattcttttg gaagattggg cggttgacgg cgaacaggga
   110881 aattcttccg aggtctatac ggacttcaat gatgcgaaac gcatactggt ccagaaactg
   110941 aaagaggagc aggaaagcgg ctgtattccg caatgggtgg atgatgagaa gttcaaggag
   111001 cattccacgg actccctcta tgaatgttac attgacggcg aatactgcga gagccattac
   111061 catatcgcaa tcgtctctca gcagttctgc gtttccaatc ggtttgtcag ggagatggga
   111121 tggctttacc aggcgtcctg ccagcttgaa gattttgtat cacaggtgtc agactgggat
   111181 gaattggatc agctgaccga tgagcaatat aaccgcatgg tccaggatcc ccgtttcccg
   111241 gaaaggctcc agaacaagct gggcaaaaac gattcttact gggaatctta ctgggagagc
   111301 gtttcagagg ttgcacatga gtttgtgagt gagtatctga aaaaggagac ataaagacta
   111361 tgaatacaag gattaactat ctctaccggg atgcagacaa ctacaagatg ccaaattcct
   111421 gtgtggttat tggcgagata agcgaagtgc aaatcgcaga agtcatatcc tgtctggact
   111481 gtggggaata tttcattccc cggcaggtgg ggcttcctga aaaacgcttt gacaggtttg
   111541 acgaggaggt ggaccattgc tggtttgaat taagtgcaga tgggtttgag gctaccgaaa
   111601 atgtttccaa tgttgacatg actgtggtgc agctcttgga gttgttccgc agtgcgaaaa
   111661 acaactggca tgatgacgtg ccattaggag gtgcagtatg agttactcag aatggcatac
   111721 ttacggctat gggatctgcg tttccgatat tacggatgag tctgtggaac gcctgcagaa
   111781 gctgatttcc ctggcaccgg agtaccaaaa gaaaatacag gcatggctgg atgagtgtga
   111841 gatatcggag ccggcctacg aggattatct ggagttcgac caggactata tgcttggcct
   111901 ggcaaccatc ttaaaagaag tgatccttga ggcagaggac attgatcttg tggcgtgcga
   111961 cagccatgac ggcacggatt atctgctcta tgtgccggat tatccctgga acatgggaaa
   112021 acacagacag ctcatgacgg aggaggccgt cgcagggctt ttccgaaaat atgtttccat
   112081 tttgacagat gaggcgatag aaatagatta ccagtctgtt gagaatggag gttaggaggt
   112141 ggcgttatgc cttataaaag cgatacaagg ccgctgacca gcatcgtctc ctatccggag
   112201 cgtggcgaag gcggcaacaa ccgttaccgg gggaactgtt cccccagact gatcgaggac
   112261 cttctggagt tcttccagcc ggcagaaatc tgcgattata tgtgcggaag cgggacgacc
   112321 aaagcggcgg cggacaagct gggtattggc agtcatctct atgacctgca cagcggtttt
   112381 gacatcatga actgcgagat cccggagcgc ccggagttta tattctggca ccctccatac
   112441 tgggatatcg tgacctattc ggatgtcatg tacaaagcgg cggatgtcca gaagagctac
   112501 ggctatgacc cgaggcagta tgacctgtca cgcataccgg actgggcagg ctttgtggaa
   112561 aaaatgaatt acgccatgat gaagcagttt tctgcccttg aaaaaggcgg caggatggcg
   112621 gtgctgatgg gcgatatcaa gaagaaaggc gtgctgtaca gtatgctttc cgagatcgtc
   112681 aaaccggggt ctttggagaa tatcattatc aaggcccagc ataactgctt ttcagaccag
   112741 atccagtata gcgggaaatt cataccgatc ttacatgagt atgtgatgat tgtccgaaag
   112801 gattcaccgc ttttggttcc gattatcatg gcaaagaaaa cggaggtcga tatccgggat
   112861 atgccgggag cgacatggcg tgatgtggtt gctgccgttt tggaacagtg cagcgagccg
   112921 gtgacgctca cgtttctcta tgagaaaata gagccgcaca aaaaagcaca ggcaaataag
   112981 tggtggaaag aaaaaatccg ccaaacgctt cagatcaatc ctacatactt ctttcatgat
   113041 ggcaggggaa tgtggtcgtt gaacagaagc gcagcatagt atctgcgtct gggggatgtc
   113101 ttttgtaggc atccctcagt tgctttaact atatgtagaa gaaggatggc ctttttgcta
   113161 taatacaaat atacttatga acaggaggcg aaatatgtct gtttattata atccggacac
   113221 gtacagaaaa attatcagaa atggtgaaga atgttgggta tatgattggg gcaaaaatgc
   113281 tgatataaat tcgttcaatt atatgctgga tcgctgggag ggcactcagc aacaattttt
   113341 tatcaatggt gagaccaagc aatattttct tattaataaa caagagcgta ctgtcatgga
   113401 aatatcaaat ccagaagtaa taaggttcct gtatgagaat ctttatatgg cgaatattaa
   113461 cgagactttt atcgaactgt ttacctcaat cggagggttt aaaacagtaa agtacgagta
   113521 gattctgatt tttttcttgg ggatgtcatt tcggcggcat ccctttttgt ataactgttt
   113581 tcaatttgga tatactaatc tccgagggtt ctatttacat tcgcacttca atagtgtata
   113641 atgtggagtg cggatgtaaa tggaaccaag gaggtgcatt gatacggaca cgatttggac
   113701 taaaattcaa acaattgcag aaaaaagcaa tgggtttgtt aagacttctg atattgaggc
   113761 ggctggaatc agcagaccga tgttgaaaaa gtatgttgat atgggaaaat tagatccggt
   113821 gcgaaaaggg ttatatactt tgaccgatga atttactgat gagtttgcgc ttcttcaggc
   113881 acaaagcgca aagataatat attcctatgg caccgcactg ttcttttggg ggatgtcgga
   113941 cagggcgccg aacatcttgg atataacgct gcctcgcgga acgaacatca gcaggttaag
   114001 gcgtgataat ccaaatttac gttgtcatta tgtacagacg gaagtttacg atcttggaat
   114061 aacagaaaca aaatcccctc aaggcgggat ggtaaagctg tatgacaggg agcgctgtat
   114121 ttgtgatgtg atccgggata aggatcaaat tgagctccag ctttttacgc aggcaattaa
   114181 ggactatttt aagacaaatc caaataaccg aaaattgctg aaatatggca aggtatttgg
   114241 aattgaagat aagataagaa catatatgga ggttttataa tgaagacacc ggaacaacta
   114301 aaaggagcca ttcgcagcat ggcagcgaag aaaaatcttc gtgcacagga agtgctgcag
   114361 atgtttttat ttgaaagaat cattgatcgt ttggcagctt catcctttcg ggacaatttt
   114421 attttgaagg gcggactctt gatatcatca atgatcggta ttggtgaacg cactactatg
   114481 gatatggaca caacggcgcg tggtattcag atggaagagg acgagatcgt ttctgcagtc
   114541 aaagagatta tcgcaatgga tgtgggagac ggaatttctt ttgaatttca gaaaattgaa
   114601 ccaatccgcg aggatgatgc gtacaataat ttccgggttc atctgcgtgc aaaatacggg
   114661 aagattgaca gcccaatgaa gatagacatt actaccggag atattattac gcctgcagcc
   114721 attcgttatg atttcccgtt tgtgtttgag gagaaaacgg tttcggtcat ggcatatacg
   114781 cttgagacag tgcttgcgga aaaatatgaa acgattatcc gcagaaacat tggaactaca
   114841 agggcaaggg attattatga tttacatact ctttaccgca gccgtaaaga tgtggttcag
   114901 atggaggttc tgagagcagc agttattcat actgcggaga aaagagattc ggttgatgat
   114961 atcatggact ggagagatat tttgaaggat atccgtgaag agccgcagtt atatcttctg
   115021 tgggataatt atgctgcgga caacaaatac attggagatt tgaaattcaa tgaagttctt
   115081 gatacagtgg acgaaattgc aaagattctt gatttgtgat atcaagtaag aaaagattaa
   115141 attttttttg aaacacttat aatagaaatc ggaatttgaa agtgaggtgt aattaaatga
   115201 ataaagaaga attacatcgt gttataggag agcaacagcc taatatatgc caagtggttg
   115261 cagtcaaaga tagcaacatt atttatagtg atacttggaa tgatttcaaa caagatgata
   115321 atgttcatat tgcttcggta acaaaaagca ttatagccat acttatagga attgctattg
   115381 acaaaggtat gattaaaagt attcatcaaa aagtattgga ctttttccct gactatgtga
   115441 ttaaaagagg agaaaagacc atacacgata ttactttata tcatttgctt acgatgaccg
   115501 caccgtataa attcaagtcg gagccttgga caaaagtatg tatgagtgag gattggacaa
   115561 aggcagttct tgatttgctc ggcggtaaaa gcgatattat aggtgagttt aagtattcaa
   115621 cactcggtat acatatattg agtacaataa taaccaaagt aagcaatatg ccaacattgg
   115681 aatttgccaa taaatattta tttaagcccc ttggaataaa gaacagacat ctttacacag
   115741 taaaaaacaa agaggaacat attggctttg taacttcaaa agaacctaaa gaaaacggtt
   115801 ggttttgtga taacaaagga acagttactt cgggttttgg cttgtgttta tcagcagaag
   115861 atatggcgaa aataggacaa atgtgccttg ataaaggaat gtatgataac aaaagaattg
   115921 tatcttctga atggattgaa aagataacaa caccatattt aactactgat gaaaaatttg
   115981 gatttatgca atatggattt ttgtggtgga ttatagatag tgaaaagaaa atatattctg
   116041 caatcggcga tagcggaaat gtaatatatg ttaataccga aaaaaatatc gttatatccg
   116101 ttacggcaac tttcaaacct ttggtatttg atagggttca gtttataaga gaatatatcg
   116161 agccatttgt tttaggatga caaattctga tttatcaatt cataaagttt tgaaataaaa
   116221 agaatattgt aacccggaga tgttgattat cagcatctct tttttatatc aaaaaggagg
   116281 tatatagtat gggatggttg ataggtatcc ttagttttat aggcggcagc gtgtttggcg
   116341 ttgtttttat gtgtatgttt cagattaacc gaacttacag aaataagcgc tcggagtaaa
   116401 catttgcagt aagaatgctc ttttctcctg gtggattgac tattgcaaat aaatctgtta
   116461 taatatttac atcagtatca agtttggcta cggctaaaca cagtttaaca gagtttatca
   116521 aagtgtcatt tgcaattttg cagatggcac tttttttgtt ttccggccaa ttcatttggg
   116581 cataagccag cagtctttcg ggattgctgg cttttgttat atatcaaggc ggcaatcccg
   116641 ccagaaaaga aaggagcatg aaatatgcaa aaacaatgtc aggatggttt ctacaccaca
   116701 ttcagttcct atccctttat gctggattat cacaaggagc agtccaagaa cagccgctgg
   116761 gtgaaagcca gggtcagtga ccttgaaatt cagccgctgg gcaaaggctc tgccctgagc
   116821 actgatctgc cggcatttgc gtcaggaacc acacaggacg ctgtggatga cacagcaaac
   116881 aatctcggcc tggccatgcg tgttgacggg gaactcttcc ccatgcgcat gacagcctac
   116941 aaaagccttt tggacagggc aaaaatcggt ggcacggcgc tccctaaact gagccgtgaa
   117001 gtgctggcgg aggtgttaaa cgcctgcctg cgtctttatt cagcggatgc acttttgctg
   117061 attcgtgatg aaaaagttgc ggcggttcat tccggggatg ctgttgatta ttcagtgcta
   117121 ccgattgatg agctgctcac tgctctgaag acaaagctgg atgcccgttt ttcaggaaac
   117181 gagtttgagt ccggctactg tgaccatgcg atggtaagcg cagcatggac gatgccagat
   117241 caaaaagagg atttgctagg agcgtacaca aagctgctgg attcccaagg aaaaacggct
   117301 atggcttcca aactgactcc gggtgtccgt tttatgagtt cggatactgg agtggcttca
   117361 gcaaaggtct cagccctact ggtgagcgga aagcgttcta tccatatcgg gggctgcatc
   117421 gcagttgacc acaggcatca gtccaaggtt tccgactttg atacagcgtt ggatcagctg
   117481 tttgcccaat tcggggatag cattgccaaa ctgcagaaac tgctggagat ccatttggat
   117541 tatccggtaa acgctatgac cagggtatgt aaaaaactta gtctgccaaa aaaggctgca
   117601 gtcgaagcta tcgccatgta cgagatggca tacggaggcg gcccggcgac agcccatgac
   117661 gtatttcttg ctatgcagga gatcccgttt attttgagga ctgaaaacac gccggaaagt
   117721 aagatgctgg tcatagaaga aaatatggct cgtgcgcttt cattcagatg gagtgattac
   117781 gaccttgcaa aggcggtgag ttactgatgg caaaaccgat tgttctttgt gataccgcag
   117841 gtatgacgaa tgacagatgg ctggaatgcc gtatgcacgg ccccaaaggg gatattccct
   117901 ataccgtggg tggaagcgat gttgcagcta tttttggtgt atcaccatgg acgactccgc
   117961 tggagctgtg gaaaatcaaa aaagggcaaa tgaagcctgc aaaaaaagcc aatgccaatc
   118021 aattgcaaat gggacatctt ttagagccgg ttgctgccta ttggtatggt gcaaagacag
   118081 gcaataccgt tacagaagac acgaatctgt atcagcacgc agatcatcct tacgcccttg
   118141 ccaatttcga ccggcgcttt acacgggcgt ccgataacga accgggaatt ttggagtgta
   118201 aaagctgtac ttatcacaaa gcggatgagt gggcagatgg agcgattccg ctctactatg
   118261 aactgcagct gaggttttat cttgcggtag cggatgtgaa cattggcgct ttttctgccg
   118321 tttggggaaa taatccggac aatgatctgg caatgcctga gattattcgt gataaggcga
   118381 aggaggatat gatctttgaa cgcctggacg aatggatctg gagccttgag aatgataaac
   118441 ccccgacaat ggcggatgtt gcaccaaagc ttgctttgga gtcactggca aggatttatg
   118501 gtgcaagtca ggcgggactt ccaacaatag agttttcccg caagtatgaa tcgcccttgc
   118561 gcagaattgc aatgcttcaa gggaaaatct ctgaatgcaa cagagaaatc aaaacatatg
   118621 aaaaggagat tgaagcacat agtgtgcgaa ttgccgaagt catgaaggag catgagcatg
   118681 gcgtcttgga gacgaccagc gataagcttc tgattgattt tgtgacaaaa acaacccgca
   118741 ggcccgattc caaagccttg aaggaaaaat acccggcaat ttatactgat gttctaaaag
   118801 catcggaaag ccgcaaggta aaggtgtcgg tccagccaat ttagaaagga gaccataatg
   118861 gaaaacattt attatgaagg ttgggagcag gagttgattt atcagttcct gccatatgac
   118921 aggtgcaaaa aaagggcgta tatctgctcg ccgctcagtg cagacacgaa tgaagggatt
   118981 gcacagaaca tgcaggcgac aagggcgtat atgttctatg ccatgaaaaa gatgcgtatg
   119041 aacgcaagtg cgccacatgc gtacctcccg atgatccttt gcgacaatat tccatctgat
   119101 agagcgcttg cccttcagtt cggtctggaa ctgctgaaag gcagcgatat cctgctgata
   119161 tgcggtaacc ggatcagcag tgggatgcgt ggggaaattg cccatgcaat ccgcctgaag
   119221 ataccgatga ttgcttttga tgagggtgta tacctggaag tacaaaagga actgacaaag
   119281 cgtgactgcg ataagcggaa agttcgcctg gacagggaga atttcttgat gggtatctcc
   119341 gctcctttgt catatcttga aaatgcggag atgttccgat gaaagaccaa ctggctgcag
   119401 cttcacaaaa aactcccgtt gtatctttgg atacgattcg ggagtttttt gtatctataa
   119461 cacgactcag agaaaggagt aaaaattttg ctatgtgaat ttgataggct catatatcca
   119521 cagagtatta cggctgttga tgccagcagt tatatgattg cactctacca tccatgtgaa
   119581 aagatcaaag atagcacagg gaacacggtt acgcaggtga aagcagtggg atattgctta
   119641 cctacatcaa gcaatctgcg ctacgacatg cttgggcatt ggagtaaaaa tcccaaattt
   119701 ggcgtccagt ttgaagtgga gagttacaac gaagtggtaa taccgacgaa ggaaggcatc
   119761 attgcctatc tttcttccgg gcagattaaa gggattggtc ccaagatagc ggagaaaatt
   119821 tacgcagttt ttggccagca gtcactggag gtactggata aagaaccgga aaggcttctt
   119881 gccataccag gtatcagtga gataaaactg aaaaaaatct atgattctta tctggttaat
   119941 cgtggcgccc gggatgtggt ggcatttctg tcaccgcatg gcattacgcc gaaccgagcg
   120001 gtacgcctat acaaagagta tggggaaaag acgatggata ttgtaaaaaa ccatccctac
   120061 cagctctgcg acatggccgg gattggcttc aagacggcag accatattgc tatgagcatg
   120121 gggtttgacc agctatcgac tgagcgtgtg gatgaaggac tcctgtatac gctggcggat
   120181 gcggaagcta aagggcatct ctgtatggag aaacatgagt ttgtgaaagc ttgtctaaaa
   120241 attctggata cgcctgcact gacttcagaa atggtggcga atcgcgccgc aaggctggtt
   120301 ttcagcggac agttggtgtc gtatcagggc aatgtgtata gggcaaaaac tgtacatgtg
   120361 gaagaacaac ttgcctccgc cattcatcag cagatgaagc accggaagat gcacagctat
   120421 ggggatttgg atgccgccat agatgcagag gagcagaagc tgaaaatgaa gtttgcaccg
   120481 gagcagcgcg aagcggtaaa gatggctctg acgcagggac tttccattat cactggtgga
   120541 cctggaacag gaaagacttt gatacaacgt gccattttag atatttatca aaaaaacaat
   120601 cccaaaagtg aaatctgctg ttgtgctcct acgggtaggg cagcaaggag gatggagcag
   120661 gcaaccgggg ttccggcttc aaccgttcat aaagcccttg ggctgatggc ggatgaggac
   120721 ggagactatg atggacccga agcgcttact gcagatctga ttgtggtaga tgagatttcc
   120781 atgctggatg tttatctggc cggatatctg tttgatgcag tgaaatatgg cgcacagatg
   120841 gtgctgattg gcgatgcaga tcagctaccc tcggtggggc ccggcgcagt gctcagtgag
   120901 atgattgcca gcggatgtat cccagttgtg cggttggaca aggtatttcg tcagaatgcg
   120961 ggaagccgca tcgcgacaaa tgcgaagctg atccgccatg ggaatgtggg tctggagtat
   121021 ggggacgact ttcagtttat caattctccc agactctctg attctgccaa actcattgtc
   121081 gatctctatc tgcgggaaac tgagaaatat ggggtcgata acgtggcgct gcttacgcca
   121141 taccggcaaa agacggaaac cggagtcaat gcgctgaatg agcacttgcg tgagaaggtg
   121201 aacccgccgg atgcacagaa accggaggtt gttttcggaa atcgaaaatt tcggtgtggg
   121261 gataaggtca tgcagatcaa aaaccatgat gatgtcaata acggcgatat cggctatatc
   121321 agaaaaatta tccgcattgg cgatgacact acagttcatg tggactttgg tgacgggcgc
   121381 atgaaagagt acgattccag tggattggat atgttggatt taggttatgc ctctactatc
   121441 cataaatcgc agggatcgga gtaccagtcc gtcatcatca atctgcagtg cgcccattcc
   121501 atcatgctga caaggcctct tatctatacg gcaattacac gaggaaaaga gcgtgtaacc
   121561 attgtcgggg aaaaaagggc gctttgtatt tcaatcaaaa gaactgacac agaaaaacgc
   121621 ggcacctgcc tcgctaagcg gctgcagggg cttgcttgaa gaaaggagca gctatggcta
   121681 caatattaga tcaatatctt gagaaacaga attctatcag gtcccagatt gccgagaaca
   121741 gtcttccgcc ggaagatttg ctgattatgc aggaactgaa ttaccgcatt tgcgtcttgg
   121801 aaacatttca gtctttttgt aagtccgcgc caatcacaat ggatacaaag gtcatgggct
   121861 atcactttca aatggtagat gcgtatgtac gttttacctt aaatgaaaga cgattcggat
   121921 taaaagccga tgcagaaggc cagaagcgtc gtgaaacagc actcagttct tttgagcgtg
   121981 ttgtacagga cggcagaaag cggttttcca gctttaaggc agggacacag gagcagtaca
   122041 aaacaagcat cagtcaatat gttcatacga ttctgccggt ttggatgcag tatcggaaca
   122101 catatatcaa tttataagga ggctataata atgagtcaga ataactcgaa tgagaaggcg
   122161 gcaatgctcg ccaaaatcga aactattcat caggtggatg gatttgatcc tacgccgttt
   122221 gctgtggatt atacagattt gggaaccgga gaggtgcgta agcgcctacc ggtcatgatt
   122281 cagatggcgt ggttccgtct gaaatatccg gaaggacgca ttgcggtaca ggtgaattct
   122341 gcaaaggatt gctttgtggc aacagcccgc gtgtatccaa actataagga tgcagcagac
   122401 tgttatctgg cagaagcgac ggcatccaga ggatattgtg aggagaaacc ttcggtttca
   122461 ccacgggaat gggcacagac tgctgctgtt ggaattgctc tacgaaatgc aggctttgga
   122521 cttcagtttt ctatggcagg tgaagatttt gagccttctg ctgtggatga gctgggaatc
   122581 tccgccgctc aacctcaaac agaatcagca gaaaaacacg aggtgtcaga accaaaaccg
   122641 gaaccggcac aagaggaata cactgtaaat gaaccggtgc aaaaggaact gactccagaa
   122701 gaaaagctgg cacaggcaat gaatgtgcct tgcccaatct caaaattcag cgggaaaacg
   122761 cttggcgagg tattatcgct ggatcctggt gcaattaaat gggtggcaac gaaattcact
   122821 ggcggcgagg aaattaaagc tgccgcgaag ctgatttgtg aattttccct tcaacagaca
   122881 attgcataac aggcgctaaa gaccagggga ttcatgcagt catcccctaa ccgggaacaa
   122941 gacgaaaagg aggacaaaaa atgtgtaaaa aaacaaaaaa gcttcgtttc aaatgggagt
   123001 ttggagatga aatggtctcg ctgcatgtca atcaatatgc ttatggcggg cggctgcaca
   123061 tcggtatgat caattacgga gaagatggtc cggaaccctt tgcagatatg acggtcaacc
   123121 taccggccta tgatctggag gataacgaag catttattaa cggagatatc agcaaagacc
   123181 tgttaaggtt tatcagggag aacaagctgg gaaaggtgat gccatatacc gtgaaatctg
   123241 gatacggcag atatgcggcg gtggcatttg atctggaaaa gctggcggaa tacgatccta
   123301 caggggttgc gtcatttaag gaaaactgtg ggttataagg aaaacagcaa tgagaagaag
   123361 cgggggacag cgtgctgttc cccgcaaatt tgaaaaagga aaggaggcag catggaaata
   123421 tttcatatga gcgatgttgt ttcgttgctg ggaatcccga tgccgccgtc tggaagaagc
   123481 tcctattatg tgcagtgtcc ctgctgtgac gaaaacccac ggaaaaagca tttgaacatc
   123541 aatttgaaaa aagaagtgta tcgctgtccg cggtgcggaa tatatggtgg tatatttgat
   123601 ctgtattcct tatataccgg agttccgcgt gacaaagtgc gaaaagctct ggttgagaaa
   123661 ctgggaatgc ctgatcaccg gcctaaaccc caaaagagga tacagccgca ggcaaagcct
   123721 gaatgcccaa taacggatgt tgatacccgt catgccacat acagtgcgct acttgataag
   123781 ctgacgctgg cggcagacca taaagagaac ctgcttggca gagggctgac ggaggatgat
   123841 attattcggc ttggatatag aacaacacct gtggtcggta tgacagcgat tgccgataaa
   123901 cttcaatctg aagggatgta cttagctggg gttcctggtt tttataggaa tgaaagcggt
   123961 acatggactt ttattcatga agagcgcggt atcttaattc caatgaggga tcggcaagga
   124021 aggattcagg gacttcagat acgcagagat aaagtgaaaa agagaaaatt tcgctgggta
   124081 tccagtacgg agcgaccgga tggttgccct gcagaagggt ggacacatct tgcaggcccg
   124141 gttaggccta tgctggtgct gacagaaggt cctatgaaag ctgacgtgat tcatgcactg
   124201 actggtctga cagtgctggc ggttcctggt gttaattctc tgacacagct tgaaacaacc
   124261 cttagcgatt tacgtgcaga aggggttgca gagattaaga ctgcttttga tatggatttt
   124321 gcgaccaact accatgtaca aaacggttac aacaatcttc tcagtcttct tggcaatatg
   124381 ggatttcagt tcggtactta cctgtgggat ccacgctata agggactgga tgattatatc
   124441 tgggaatatt gttttcaaaa gaggcattaa aactggggtg gagcaaaaat gcttcaccct
   124501 tttttcgcgc gttttttgaa tcgaatgcgt ataaaagttg taaatgcgca tggcgaatcg
   124561 tgtaatgata aatataagaa taatttttgc aggaggtata catatgttaa tcgcaatcga
   124621 ccacggcaac tacgccatta agactacaaa ccattctttc atttccgggc tttctgagca
   124681 cacggttaag ccgcccatga cagatgagat tttggagtat ggaggaaaat actggacttt
   124741 gagtggacgc cgtctgagtt atatgaggga caaaacccag gatgaccgtt atttcatcct
   124801 gacattattt gcaatcgcca aagagctgga gaatggaggc aattatacgc cggtcgaaca
   124861 ggttcagctg gctgttggac taccacctga gcattttggt gctttaaaag acagattcgc
   124921 caattatttc aaaagagacg gaattatcaa atttgtttat ggtgacaggc catacagcat
   124981 taagatagac cgtgttatgg tgttcccgca ggcatatgcc gctgttattc cccagagcag
   125041 tctggtggtt catacggttc gtatgtttat cgttgatatt ggtggttata ccaccgatgt
   125101 gctgctcttg aagaatggaa aaccagattt gcagttttgc cggagcctgg aaactggtat
   125161 tattactatg aacaatgaaa tcatacgaaa ggttggtgcg cttcatgata tgcgtatcga
   125221 agatgagcat attagtgcag tgcttcagtg cgagaatacc attctgccgg aggatgtcaa
   125281 ggagacaatt cgtgaagcga cgaagttaca tgcacaggat attctgaacc agcttagaga
   125341 attgcaagtg gatttgcgtt ctaatccggc agtttttatc ggagggggaa gtatactttt
   125401 aaggcctttc ctgataaatt cgcctctggt agcaaaggcg gattttgtgg aatctccgaa
   125461 tgcgaatgcg ctgggatatg aaatgttggg caggaaaacg ctttctcttc gtccacttgg
   125521 ataagggacg gagggacaat ataatgaaaa aggatggaaa ataccgcttc tcgcttcaat
   125581 ttagcgcaga ttcagaggaa caggtaaggg ctggagagtt gctcgaaagg ctgggaaaca
   125641 ggaaaagtgc ggttgttgtt gcagccttga atgattatct tgattctaac ccgaatttac
   125701 aggatgcaca ttgtaaaata gaagtcaagc taacacctgg ctataatcga gatggaattg
   125761 aggaaatcat acggcgcatt gttgaggaaa gacttgcagc ggttcgccgt gacgaacccc
   125821 agatggcttt ttcagaggag agtaagacag atactctgga ggcagacatt gcacagatgt
   125881 tggacaacct ggatatgttt tcatagaccg acagtgcatt taataggttc aacaatggct
   125941 ttttaacgct tgaaaattaa aaaagtccat gtaaatgagt tgatattcca cttatagtgc
   126001 gtatattgga atatcaagtt cttcaattcc attgtcagat tatataaaat agaaatgtaa
   126061 ggagaatgta aaaccatgga ctatgttatt ttaggcaaga atattcggaa gtaccgacaa
   126121 atgcgcggaa tgcgccagaa agatttggcg gagatctgtg attgcgggaa tagccatatc
   126181 ggccaaatag aaaatgcgag agggattcca agtctggata tgattgtacg gattgcaaat
   126241 gctctttcag taacagtaga tcaattgctc aaagagtatt attcagaacc tgagaaggtc
   126301 tatttgcgag agattgcgga acgtattgag aagtatccgg taaagcagcg tgtatacgct
   126361 tgcgaaggtc tggctgaatt tttgaatacc attgaaaaat tcagtcaaac agatgaataa
   126421 tgcgcatata accaaacaga aaaaagttta cgctttatag aacggctatg ctacacgaaa
   126481 gtgcagtatg gtcgtttttt gttatctgaa tacaaggagg tggtcccatt ggattaaacc
   126541 attgacagct gcaaaaaggt aacaagtcga gaaaattaag gcacgccatc acggcgtgcc
   126601 ttgacttttg ccctggtggt gtgccccgta aggaggtaca aagatgaacc accagcagaa
   126661 gtctgaacgt gacgagctgc gagccagatt tacagtttgg ctggaaacta cagtttaccg
   126721 tgccagactc aaatatttgg aacgggagaa acccaaggta gatacagttt caattgagga
   126781 attaccagag agcgctttat ctgttcttga acaaacctca cattgtggaa tatcaggatc
   126841 tgcttttgat tttgaagagg aacggttggc agaggcgttt gcaaaactgc caataaaacg
   126901 gcaggagata ctgacaatgc tgtttgtgga ggagcgaaaa ccggaggaaa ttgcccggct
   126961 attaaattgt tctccgcagc atgtttacga ccagcgttat caggcattga agaagctgcg
   127021 catagcattg acaaaggacg gtgataaatt ttgaatcgca tagaatttga aaagatgctt
   127081 caggcggcag tttccggcag tcatgaggct ctggagcaac tatttttgct gtatgcaccg
   127141 ttaattgata aacatagtaa aattgacggt cagatagatg aagatttaag acagtatcta
   127201 ttgatacata tcgcgctcaa tatttcaaaa tttgtcattt aggcgaagac ggtctcaggt
   127261 ttttctgagg ccgttttcat gtttttataa attctgttcg agatttgaga gatgatgagg
   127321 ctttttatag gtaaaggcat ccccatgcca tggcaccttg acaacttcat agcttccaac
   127381 tctacgttcc cgaaccgaga agcggctatc cgtttgtgtg gcgagcaaaa ccatgagcac
   127441 tgatatacgg gcggcacccg tataacgcaa tgatccggcc ggggataatg atacttccgt
   127501 aattcgcggc ccggccataa ggcaggcggg gagatgagat atctatggat ctgcagccac
   127561 agacggagaa ggaggcggtt attttgaaat aaattatttt ttatagtaag gaggctgatt
   127621 atgggctaca aaaggaaaga tgtagagcag acgaagcagt atgcaaaaaa gtttgtgcgc
   127681 tacaaagaag gagcggagaa atacagcctt ggactgacaa agtttcagga actcgcaaag
   127741 gaggcaaagg ctgtctataa gattgatggt attgctttag ttaattgcga aatttttgaa
   127801 aagtttttgg agtcattccg agaattttga agtgggtggt gctttatggc ttttttacat
   127861 aaagggactt tatgtgcttt agggcttgac tttctgtctt acaataagta taataaaata
   127921 catgtggagg tagcggtatg acgaaaatgg gtagaccgaa ggtggacaac gcaaagcgta
   127981 aatccattac ggttcggtta tcagacaaaa cctatgatga gttgaccagg tatgctgctg
   128041 aacacaagat gacaaagaca gaggttgccc tgcgaagttt ggaagagtat ttgtccaagc
   128101 aaaaataagt gctgtgagtc ccttcttcat tattgaagga ggtaacagac atggcaaaga
   128161 cacggaaaga tgagcgggga agagcactca ggaaaggaga agtacagagg gcatcagaca
   128221 agcggtatat gtacacctat acggatccat tgggcaggcg taggtccata tacgcgcagg
   128281 atttggcaac gcttcgagaa aaagaaaagc agctgatgaa ggatcaattg gatggtttgg
   128341 atctgtatgt tgcaggtaag gccaccataa atgataccta tgatcggtat atctctacga
   128401 agtatgattt gcgggcgact acacgcagca attacgatta catgtataat cgctttgtca
   128461 gacatacttt cggtaagaag aaaatcgcag acattaagta ttcggacgta ctccagtttt
   128521 actgctactt gcttaaaaag gaaaagttat cgttagggac gctggactca gttcataccc
   128581 tgcttcatcc gacattccaa ttggcggttc gagatgaaat cattcggaaa aatccgtcag
   128641 acggagttat gaaggaagtc agcaagaagg cagataagac aagaggcgtg agacatgctt
   128701 taacgagaga gcagcaaaga gcatttatgg agtatatttc caatcaccca gtgtattatc
   128761 attggtggcc gttatttacg gttttgcttg gaactggctg ccgaattgga gaagcactgg
   128821 gacttcgatg ggatgatctt gattttgata atcggattct gagtattaat cacagtctgg
   128881 tttattaccc agttggagaa tcgagaaagt ctgttctgcg gatatcaaag ccgaaaacgg
   128941 aggccggtat cagaacgatc cctatgcttg atattgtacg tgatgctttt cacatggagc
   129001 acgaggagca ggaagaaaca ggattcaacg agaccgagat tgatggaatg actggttttg
   129061 tatttgtcaa tcgttttggc tcagttttaa atccgcaggc tgtgaatcgt actatcaagc
   129121 gaattatcag tgggtataat gcggaagagg ttatcaatgc taagcgtgag cggcgagagc
   129181 cgattattct tccagatttc tcctgtcatc acttgaggca taccttttgt acaagactct
   129241 gtgagcatga aacaaattta aaagtgattc aggcaattat gggccatcgg aatatcgaaa
   129301 cgacgatgga tatctatgcc gaggccactg atcaaaagaa gcaagaatct tttgaaaact
   129361 tatcaaaatt ggacatcttt taggaagggt tctcagcgtg agaagctgac aacaaacttt
   129421 ctgcgaagac aacagtttga caacaatttt cttttttccc tgatgtaata cgaagcattg
   129481 tgaagaatgg ggaagcaagg aaatgaaagg atttgaaccg atatgatggc atgtaagttg
   129541 ggtggaaaac ttttcccaac aatgagataa tcgaataaaa gctgtctaag gaattcctta
   129601 ggcagctttt gctgtgtaga aaggaataaa taataacgaa agttcaaaag ctaatgccga
   129661 ctattctaga cattaaagaa gacgaaaacg aacgataaag aaagaaaagt tatgaataat
   129721 cgtttattga aaaaaacttg aaaaaataca aaaaattcgt tgaccaagct aaatttagtt
   129781 ggt
//
LOCUS       DAZUMK010000002        58700 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000002 DAZUMK010000000
VERSION     DAZUMK010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 58700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.002"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(91..1110)
                     /locus_tag="ACG808_000160"
     CDS             complement(91..1110)
                     /locus_tag="ACG808_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5045823.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(1132..2139)
                     /locus_tag="ACG808_000161"
     CDS             complement(1132..2139)
                     /locus_tag="ACG808_000161"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFS5045824.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(2132..2797)
                     /gene="pgmB"
                     /locus_tag="ACG808_000162"
     CDS             complement(2132..2797)
                     /gene="pgmB"
                     /locus_tag="ACG808_000162"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFS5045825.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            complement(2790..5084)
                     /locus_tag="ACG808_000163"
     CDS             complement(2790..5084)
                     /locus_tag="ACG808_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFS5045826.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            5344..7509
                     /locus_tag="ACG808_000164"
     CDS             5344..7509
                     /locus_tag="ACG808_000164"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFS5045827.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            7813..8646
                     /locus_tag="ACG808_000165"
     CDS             7813..8646
                     /locus_tag="ACG808_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFS5045828.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(8748..10448)
                     /locus_tag="ACG808_000166"
     CDS             complement(8748..10448)
                     /locus_tag="ACG808_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045829.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            10955..12115
                     /locus_tag="ACG808_000167"
     CDS             10955..12115
                     /locus_tag="ACG808_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS5045830.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            12131..12574
                     /locus_tag="ACG808_000168"
     CDS             12131..12574
                     /locus_tag="ACG808_000168"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5045831.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(13057..13587)
                     /locus_tag="ACG808_000169"
     CDS             complement(13057..13587)
                     /locus_tag="ACG808_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFS5045832.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            13692..14243
                     /locus_tag="ACG808_000170"
     CDS             13692..14243
                     /locus_tag="ACG808_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS5045833.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(14396..14869)
                     /locus_tag="ACG808_000171"
     CDS             complement(14396..14869)
                     /locus_tag="ACG808_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS5045834.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            15007..16479
                     /locus_tag="ACG808_000172"
     CDS             15007..16479
                     /locus_tag="ACG808_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFS5045835.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            16476..17186
                     /locus_tag="ACG808_000173"
     CDS             16476..17186
                     /locus_tag="ACG808_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFS5045836.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            17538..17822
                     /locus_tag="ACG808_000174"
     CDS             17538..17822
                     /locus_tag="ACG808_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFS5045837.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(18103..18522)
                     /locus_tag="ACG808_000175"
     CDS             complement(18103..18522)
                     /locus_tag="ACG808_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFS5045838.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(18524..19108)
                     /locus_tag="ACG808_000176"
     CDS             complement(18524..19108)
                     /locus_tag="ACG808_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFS5045839.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(19155..20630)
                     /locus_tag="ACG808_000177"
     CDS             complement(19155..20630)
                     /locus_tag="ACG808_000177"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFS5045840.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            20811..21122
                     /locus_tag="ACG808_000178"
     CDS             20811..21122
                     /locus_tag="ACG808_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045841.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(21237..23573)
                     /locus_tag="ACG808_000179"
     CDS             complement(21237..23573)
                     /locus_tag="ACG808_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFS5045842.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            23715..24383
                     /locus_tag="ACG808_000180"
     CDS             23715..24383
                     /locus_tag="ACG808_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5045843.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            24386..25429
                     /locus_tag="ACG808_000181"
     CDS             24386..25429
                     /locus_tag="ACG808_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5045844.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(25666..26547)
                     /locus_tag="ACG808_000182"
     CDS             complement(25666..26547)
                     /locus_tag="ACG808_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS5045845.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            26706..27434
                     /locus_tag="ACG808_000183"
     CDS             26706..27434
                     /locus_tag="ACG808_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFS5045846.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            27590..27907
                     /locus_tag="ACG808_000184"
     CDS             27590..27907
                     /locus_tag="ACG808_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS5045847.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            27900..28508
                     /locus_tag="ACG808_000185"
     CDS             27900..28508
                     /locus_tag="ACG808_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFS5045848.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            28563..29354
                     /locus_tag="ACG808_000186"
     CDS             28563..29354
                     /locus_tag="ACG808_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFS5045849.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            29492..29980
                     /locus_tag="ACG808_000187"
     CDS             29492..29980
                     /locus_tag="ACG808_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFS5045850.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            30031..30903
                     /locus_tag="ACG808_000188"
     CDS             30031..30903
                     /locus_tag="ACG808_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFS5045851.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            31079..31441
                     /locus_tag="ACG808_000189"
     CDS             31079..31441
                     /locus_tag="ACG808_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS5045852.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            31450..32202
                     /locus_tag="ACG808_000190"
     CDS             31450..32202
                     /locus_tag="ACG808_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5045853.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            32216..34018
                     /locus_tag="ACG808_000191"
     CDS             32216..34018
                     /locus_tag="ACG808_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5045854.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            34295..35335
                     /locus_tag="ACG808_000192"
     CDS             34295..35335
                     /locus_tag="ACG808_000192"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFS5045855.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(35387..37531)
                     /locus_tag="ACG808_000193"
     CDS             complement(35387..37531)
                     /locus_tag="ACG808_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS5045856.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(37888..39261)
                     /locus_tag="ACG808_000194"
     CDS             complement(37888..39261)
                     /locus_tag="ACG808_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5045857.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(39261..39656)
                     /gene="rbsD"
                     /locus_tag="ACG808_000195"
     CDS             complement(39261..39656)
                     /gene="rbsD"
                     /locus_tag="ACG808_000195"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFS5045858.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(39684..40601)
                     /gene="rbsK"
                     /locus_tag="ACG808_000196"
     CDS             complement(39684..40601)
                     /gene="rbsK"
                     /locus_tag="ACG808_000196"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFS5045859.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(40709..41722)
                     /locus_tag="ACG808_000197"
     CDS             complement(40709..41722)
                     /locus_tag="ACG808_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFS5045860.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(41803..42402)
                     /locus_tag="ACG808_000198"
     CDS             complement(41803..42402)
                     /locus_tag="ACG808_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFS5045861.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            42588..43289
                     /locus_tag="ACG808_000199"
     CDS             42588..43289
                     /locus_tag="ACG808_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5045862.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            43299..46010
                     /locus_tag="ACG808_000200"
     CDS             43299..46010
                     /locus_tag="ACG808_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFS5045863.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            46461..48089
                     /gene="treC"
                     /locus_tag="ACG808_000201"
     CDS             46461..48089
                     /gene="treC"
                     /locus_tag="ACG808_000201"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFS5045864.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(48149..49330)
                     /locus_tag="ACG808_000202"
     CDS             complement(48149..49330)
                     /locus_tag="ACG808_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS5045865.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            49582..51105
                     /locus_tag="ACG808_000203"
     CDS             49582..51105
                     /locus_tag="ACG808_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFS5045866.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(51174..51902)
                     /locus_tag="ACG808_000204"
     CDS             complement(51174..51902)
                     /locus_tag="ACG808_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFS5045867.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(51915..53195)
                     /locus_tag="ACG808_000205"
     CDS             complement(51915..53195)
                     /locus_tag="ACG808_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFS5045868.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(53336..53938)
                     /locus_tag="ACG808_000206"
     CDS             complement(53336..53938)
                     /locus_tag="ACG808_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFS5045869.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            54183..54389
                     /locus_tag="ACG808_000207"
     CDS             54183..54389
                     /locus_tag="ACG808_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045870.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            54701..55420
                     /locus_tag="ACG808_000208"
     CDS             54701..55420
                     /locus_tag="ACG808_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFS5045871.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(55501..56184)
                     /locus_tag="ACG808_000209"
     CDS             complement(55501..56184)
                     /locus_tag="ACG808_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFS5045872.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            56402..58588
                     /locus_tag="ACG808_000210"
     CDS             56402..58588
                     /locus_tag="ACG808_000210"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFS5045873.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNISVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT        18812 a        10450 c        11860 g        17578 t
ORIGIN      
        1 cagataaaca atgtgttaga agtagtatct tcttttacat aaaaagctga ccataggata
       61 aatggtcagc gtatttagtg agtcagctat ttagtatggg ttttctaaag gaacgactgt
      121 ctctcttttg atcaagcgat gagggatgac caaacgtacg ccactgctca attcttcatg
      181 gatcaattcg cgcagctttt gcatagcgac acgtcctaat tcggagatat cgatatcgat
      241 acttgtaagg taagggtgtg ttaatgttga gaagatcgag ttgttgaagc taatgacgga
      301 gagtgtctct ggaatgacat agccatacat ttgcgccaac tgcatcgtgc gaagggcgaa
      361 gatatcgtca atgaccacca atgctgtggc ttcgttttct ttcaacgttt tctcaaagga
      421 cagataatcc tccggatgct ctagtactac actggataac gcaggaagac cagccatcat
      481 cattgctttt tgataaccga aatatcgttc gaaatacaaa ctttcatgag tcgtgtttgt
      541 cacaaacagg atattcttat gtccattctg gatcaaaaat tctgttgctt gtttacctag
      601 aagctggtta tcattatcta cataaacgat ttcgttctca tgatgataag gctgtccgat
      661 caaagtgaaa gggatgtggt tttggtataa ataatcaatg acagtatctt gactatctga
      721 atacactaga ataaaaccat ctacttgttt ttgtaaatgc atacgctgga cgttctctaa
      781 cagcaattca aaatttttag cagtagcgat tgctgtagtc atatcataat tccgcgcttc
      841 ttcgttgatt gcttccatga tctcaagata aaatgggtta ccgatccgct cttttgaatc
      901 aagagggggt aagattacgc cgatggcact ggaaatgcgt tttcctagat tgcgggcagt
      961 catgttagga acataaccta gttcatccat aatttttcgg actttcttct ttgttgtctc
     1021 ggaaatacta ggatgatcgt tgatgacacg agagacggtc gaggtggcaa caccggcttt
     1081 tttcgccaca tcttttacgg taatagccat gataaaaaca cctttctctt gttattgtgc
     1141 agagaaccga taaatcgtct gactgatccg ttcttgacca ggatggaggt tgatcgatcc
     1201 aaatttgggg aaatgaacga tatcagggat ttcttgcggt tcgattgcta atccgtagtt
     1261 tgaatgcatc tgttttccat tgacagagaa atcagcttcg aatccagtcg tagaaaataa
     1321 taccatactt ttattagaag ttgctatagt catttggcga ccagaatgct tctcgaataa
     1381 ttgtaaggaa gttttggaaa gcctaggatg atgcaaaaga aatacatcat ctagtccatc
     1441 tggataacat gcaaggatct cttgcagtgt tggggatttt ctaaagtcga ttggcagttt
     1501 ttcttttttt gtccaatttc cagtaggcag tttttcttga tctaattcaa gcacaccatc
     1561 taaaaagacc gttaactgat gttcatagat gtctcttgtt ccatttcctg aaaggttgaa
     1621 gtaggcatgg ttggtcggat tgcagatggt ttcaacaagg ctactagcag aggttgtcat
     1681 ctctaattcg ttttttgtta aacggtaagt gatcgtagca gtgattggtc ctggataggt
     1741 agaaaattca tcttttaagt aaaagaccac acctattgat gatggagttt tgaaaacttc
     1801 tacttcccaa aattggaaag accagccttt cgtgccaccg tggatatggt gtgatccgca
     1861 gtttttctcc aatctatgct caccccaaga agcatcccgg atccttccgg ctacaggtcc
     1921 gacagttgct ccaaaaaagc ttttgtctgt tagaacatct tcgaaacgat cgtaagacaa
     1981 taaaacattt tctgattttc cttctttgtc tggtgcgaag atttggtgca ggcgggcacc
     2041 gtaattgaga agaacgactt ctaagtagtc gttcttcaat gtgattttct ttatttcagt
     2101 ctctggaact ggtacgacgg agatctctgg attatccatg gtcatgccac ttttctttca
     2161 agtaatcgat cgtaagaaaa gtcgtatcag ggacaagcgg gatctcctct ccaaggtctt
     2221 cagcacgtcc cacgccaatt ggttgagcgc cacttgagag gatggcttgg ataccggctt
     2281 gcgcgtcttc gatcccgata cattcttctg cttttagtcc gacagctttt gctgcaagga
     2341 ggaagatatc tggcgcaggt tttccgtttg ttgcttctgc cgggtcggca atagcatcaa
     2401 agtaaggtgt aagacccatt ttttcaagta ggaatggccc gtttttactt gcagaagcaa
     2461 gagcaatctt gattttttct tcttttaaag aatccagtaa aggcacgacc ccggggaaga
     2521 catcttttgg ttcgatcgtc tggatcatct ctaaataata atcgtttttt cttttcgcta
     2581 attctgcaaa ttcttccttt gaaaaagagt gttcttttcc gccataagct aaaagcaaag
     2641 agagtgagtc ttctcggctg acgcctttta gctgttcgtt gaatgtgtgg tcgatcgtaa
     2701 tgccgatttc ctgaccaagc tttcgccaag cacgataatg gaattcagca gtatcggtaa
     2761 tgacaccgtc taaatcaaac aatacccctt taaacatttg cttcctcctt tcttagcgtt
     2821 atttctagac gatccgctaa agaataagac tgttcataga cgtaaatttc tagaggagaa
     2881 ccttctaata actcgaaagt aacttgttca tctgtgactg agatgaacaa taaacgtccg
     2941 cgataattga tatggaaaga ataagctttc caagtttttg gcagaaatgg tgcgaatctc
     3001 aactgatcat gggcagtttt catttgagca aatccttgga caatcgacaa ccagctacca
     3061 gtcatggaag tgatgtgcaa gccgtcttct gtgtcgttat tgtaattatc caaatccaag
     3121 cgagctgttc gctggtacat ttctacagct ttgctttcca ttcctaattc tgctgctaaa
     3181 atcgaatgga tacttggcga aagggaagac tcatggacag tcattggttc atagaaatca
     3241 aagttttttt gcttttcttc ttttgtaaaa gtatcattga agaaataaat cccctgtagt
     3301 acatctgcct gcttgatgaa gcaagatcta aggattttgt cccaagacca atgctgattc
     3361 agaggaagat cgcttgtggg aatatcggat acaggcatga gatctttatc taagaatgtg
     3421 tcatgctgga caaagattcc taattcttcg tcaactggga agtacatgtt ctctgcgata
     3481 tctttccatt tggctagttc ttcttcgctg atagtgatag cagcttcttt ttgataagtt
     3541 aaataatttt ctcgcgtgta acgcaagacc caagctgcga tcgtatttgt ataccaattg
     3601 ttattgatat tgttctcata ttcgtttggg ccggtcacac cgtggatcat gtatttattt
     3661 gctcgttttg aaaaatggac acggtctgcc cagaagcgtg cgatttctgc taaaacttcc
     3721 agtccttcat ttttcaaata ttccgtatcg ccggtgtaat tcgtataatt gtagattgca
     3781 taggcaatcg cgccatttcg gtgaatctcc tcgaatgtga tttcccactc gttatggcat
     3841 tcgacaccag tgaaggtgac cataggataa agcgcgcctt ttaatccttg ttgctgagcg
     3901 ttgtggattg cttgcggcag ttgattatgc cgatatttca gcagattctt agttacttca
     3961 ggtttggcta aagcaagata aagagggaca gcatacgctt ctgtatccca gtaagtggct
     4021 ccgccgtatt tttctcctgt gaatcctttg gggccgatat tcaatcgttc atcttcgccg
     4081 tagtaagtag agaatagttg gaacaaattg aatcgaatac cttgttgtgc ttcttcgtct
     4141 ccttcgatga ccacatcggc aagcttccat cgttctgccc aagccgctgt ttgctcttct
     4201 tttgcttgga tataattgtt tgtctggttc gttaattctg agacagcttt ttctgcttgc
     4261 tgcgcttctg gaatatctct actagtaaga acgataattt ctttttccac tgaccatgtc
     4321 tcaccttgag ataattctgc agacaagcaa ccatgtaatg ctaaagtatc ttcgtgatag
     4381 tcagggatga ctgattttcc attaacgaaa tggcgcattg ctgcagtaac agtaaattgt
     4441 tcaatcccaa agttgtttgg aatagtttta gtcgtcaaat agccgatttc ttctatagtt
     4501 ccacgatcaa tctcattcca aaaatgttca tcatagttgc tgtcttcgtt ttgaacattt
     4561 ccatccaatt ttgatacgag tttgactgtt ccgctacctt caagcatttc tatagttaaa
     4621 tagatataag ctgattcttt tttgacaatg ctcagaaaac gttcgaagga aatgcgtact
     4681 ttattagtag aagtagtgat tgtgaatgta cgagataata caccattttt catatctaat
     4741 tcccagtaaa agtcttctgg ttccaaagaa gccaagtcga tcagctgatc gttgatcgaa
     4801 atatctatcg cgataaagtt caaggcgttg atcacttttc caaagtattc tggataacca
     4861 tttttccacc agccgacacg tgtcttatcg ggataccaga cacccgccaa ataagtacct
     4921 tgatggtggt cccctgagta agtttcttca aagttcccgc gcatccccat ataaccgttt
     4981 ccaatacttg ttaaagattc ttgcagtcga agattttttt tatctaattg tgtcgtgctg
     5041 attttccatg gatcaatagc aaatagtcgt ttgatctgtt tcatttttct cctcctaatg
     5101 agctgtttta tacgttcata ataaacgcaa ccgattgcac aagtcaatag acaaaagaaa
     5161 atatttcata gaaaaaagct taaaaaaata aatttttagg agaaaattat agaagggaac
     5221 acacgtatta taggttttat agaatggtat agacagaaaa ataaaaaata aagcatttac
     5281 aaaaaagaaa acgcacgcta taatacgttt aacgaaaccg attgcgtaaa ggagctaaac
     5341 ggaatgaaaa agatatttag ttttgaattt tggcaaaaat ttggaaaggc tttaatggtt
     5401 gtggtagcag ttatgccggc agctgggttg atgatcagta tagggaaatc aattcccttg
     5461 atcaatcctg attggacgcc gcttgtcaca acgggcggag taattgaaaa tattggttgg
     5521 gcaattattg gtaatcttca tattttattt gctttagcaa taggaggaag ttgggcaaaa
     5581 gaaagagcag gcggagcatt tgcagctggg atctcattta ttttaatcaa tcggatcact
     5641 ggggctatct ttggtgtcac aagtgacatg cttgcgaatg aagatgcatt tacccacact
     5701 ctgttcggta cgaagatcat ggttaaagga ttttttacaa gtgtattgga agcaccagct
     5761 ttgaatatgg gagtattcgt agggatcatc gctggatttg tcggggcaat ggcttataat
     5821 aaatactata attatagaaa attgccggat gctttgtcat ttttcaatgg aaagcgcttt
     5881 gttccgtttg ttgttatctt atggtcaaca atcgtggcat tgattcttgc tgtggtgtgg
     5941 ccaaacgtac aggctggaat caataatttt ggtttatgga ttgcagaatc acaagaaagc
     6001 gcaccgattc tagcaccgtt tttatttgga acattagaac gtttactatt gccatttgga
     6061 ttgcatcata tgctgacgat tcctatcaac tatacacagc ttggcggatc gtatgagatt
     6121 ttatcaggcg cacaggcggg gacacaagta tttgggcaag atccgttatg gttggcttgg
     6181 gccactgatt tagtgaatct taaaggtgca ggggatatgt cgcaatatga gtttgtgttg
     6241 cataactgga cgccggctcg tttcaaagta ggacaaatga ttggttcttc aggtatcttg
     6301 atgggaatgg cttttgcgat gtatcgtaat gtcgatcctg ataagaaagc aagatataaa
     6361 tcaatgtatt tttcagcagc gttggctgtc tttctaacag gtgtaacaga accattggag
     6421 ttcatgttta tgtttgctgc tgtgccatta tatgtcattt attcagtgat ccaaggggct
     6481 gcatttgcga tggcagatat cttgccattg cgggtccatt catttgggaa tatcgaattg
     6541 ctgactcgga caccgttagc tatcaaagcg ggtctaggtg gagatcttat caacttcgtg
     6601 ttgtgtgtga tcgcttttgg tgttgttacg tatttcttag caaatttctt gatcaaaaaa
     6661 ttcaactttg ctacaccggg acgtaacgga aattacgaca atgacagtga agaaaccgtt
     6721 gtatctaaca gcggaacagg aacagcagat cagcaagtgg tccagatcat tcatttgctg
     6781 ggaggaaaag aaaatatcaa agatgtcgat gcttgtatga ctcggttacg cgtaagtgta
     6841 aatgatcgag aaaaagtagg gactgaagaa gcctggaaac gagcaggggc gatgggattg
     6901 atcgtcaaag acaatggagt ccaagcagtt tatggaccaa aagcagatgt cttgaaatca
     6961 gatatcgaag atctgctgca atcaggagca gagattccag agccagaaat ggtttctaca
     7021 ggaaatggac aagcaggtag taagcaatac ttaggtatag aacaagaact agtttccgca
     7081 gcttctggag aagtgattcc tttatctgaa gtaaaagatc cggtattctc acaaaaaatg
     7141 atgggtgacg gctttgctgt tattccaaca tcaagagaag ttgttgcgcc gatcgctgga
     7201 aaagtgacaa gtattttccc tagcaaacat gcaattgggc tggaaacgaa agatggtatc
     7261 gaagttttga tccatatggg aatcgataca gtccaaatga atcagccagc ttttgagatt
     7321 gctgtcaaag aaggccaaga agtaggaaca ggaacgaaat tagctgagat ggatcttgat
     7381 gtgatccaaa acgaagaaaa agatccaacg atcatgatcg tatttacaaa cgataaagta
     7441 gaagaagtag tgatcaagca attaggtaca gtgacagctg gtaaagtgat cggctccatt
     7501 aaaatgtaag gagaggttgt gaaaaagctg tcctgcatca agtcataaga acagccaaac
     7561 aaaaatagct cctcctattt ttcgtttggt tgggcttatt accgagaagc agcagtttga
     7621 atactgttta gtggagaggt cgtgaaaaag ctgccctgca ccaagtgata aaaacagcca
     7681 aacaaaaaat agctcctggt ccattgagct tattactgag gagttagcag gagaacacta
     7741 tttgctggag aggttgtgac aaaaattttg tcacaaccct tttgtttcga aatgaagaca
     7801 aggagaaaac caatgaaatt catgacactc aacacgcata gctggcttga gccagaacca
     7861 gagaaaaaat tgcaagaatt agcagataag atcctgttag aagattatga agtgattgct
     7921 ttacaagaaa tcaaccaatt actggaatct gaagaagtag aacctgcgaa gctaatgaag
     7981 ttctgtccgg taaagaatca agtacctatc cgtaaagata attttgccta ccgtttagtt
     8041 cagcttttaa aggaacacgg aaaagagtat ttttggagtt gggaaatgag ccacatcggt
     8101 tatgataaat atgaagaagg aaatgcactg cttacgaaaa aaacattgga aagtcaggtc
     8161 cttaccgtct cccaatttca aaagaaagaa gattatcaga cacgcaagat cttgattggg
     8221 aaaacaaaga tagatgatca ggacattttt gtctcgagct gccacttttc ttggtggaca
     8281 gataaaaaaa gtggctttta ttttgagtgg aaaaacttgg aaaactactt tttggaaact
     8341 cgcgtaccac tttttttcct aggtgatttc aataatccag tagactctca aggttattat
     8401 actgtaagag aaagttgcct tcttcttcaa gattcgtatg ttgtcgcgaa tgaaaaagga
     8461 aaagcagcta ctgtggaaaa gaaaatagat ggttgggaac aaaatacaga aaaattgcgt
     8521 atagatttta ttttcgtccc tgaaggaatg caggttaaaa aatatcaacg gatttttgat
     8581 gggatcgata gtccaatcat cagtgatcac tacggggtgg aaatcgaatt agatgtgaac
     8641 gaataaaaaa tataacatat aacataaaaa aactgattaa aataaaactc tccaaacgat
     8701 tataccgttt ggagagtttt ttgatcggat gtatttatcc cttttgccta agcacgtaaa
     8761 agcttgtatc tatgataaat aaataaacat agcaaggtaa accagccaat caaagtaaga
     8821 gaaagaatga aatagaaata gattggcgtt tgataatgaa gcgtgagctc gttgattcct
     8881 ttttgctgct tcacgattgg ggtcccgata tctgttactt cataatcagt caatgttttt
     8941 ccattttgct gtagcacagt ccgatcgtag acgataacag ggatatgaac agttttgtcc
     9001 ccagtgtttt tccacttgat tgtcaaaaga ccgcctggat gtgtttttat aaaagttgtt
     9061 tcgttttcta gtatttcttc gttataccgg tcgtatttat tgtcttttgt ctccttatag
     9121 attggcagat agtcaggtgt ggttttttgg aaaacgaaca agctggtaga catatctttg
     9181 ctgaagaatg ttttccttgc ttcttccggc gtatcgaata gataagtatg tcgactgaca
     9241 aatgtttcat tttcccactt ttccagatgc tggtataagt tctggatcgt ctgtgctgaa
     9301 aaaataagaa tgccaaatat acttaaaaga cggaaccact tttgttcaga ccactggttc
     9361 agtgaaaggg caaggtaaag tagaagcaat agcgtaaatg gcacgaaaaa tcgaaatgga
     9421 aactggatca aactaataaa aggcacattt ttttcgatca atgtcgtcca tggaatcaag
     9481 ctagtagaaa gaaaaaagaa aacaagactt gtgtacaaca cgattttttg gaataaggtg
     9541 ttcttttgtt gaaagaaaat aaaaagcaaa aaacctgata atatcaatat aggaaaggcc
     9601 ttagggtaat ggagccaata actgccatcc agatctatcg ccttttgact catttctctg
     9661 ttaacaaagg gaggaagaat cttattccca ctgtaaacaa tagctaatcc tgcccaaata
     9721 tttgctgtca gaacaaaata aagcaagacc gacttaatca gttgaagcag tgcttttcct
     9781 ttttcagtct ggttaaaaaa gtaataaaga taaaaaggga tatacatcaa agctaacatg
     9841 atcgaagaca gtaaatggat ctgtgtcata agtcccactg agacagcgac tttgataaca
     9901 gggaactttt tatgaaggac gaaatcagca atagggatca tacataaagg ataaaaacaa
     9961 gtcccccagc tagtgaatcc ttggttgata gaccagtatt gaatagaata cgtactcatg
    10021 aataaaacgg ctactggtac acttacccaa tttcgaacat tgacatatct cagtaaaatc
    10081 agcatagaaa atcctgaaat aagataaaga atgaaatttg aaagaatctg ataatgaaac
    10141 cagctacctg caagcaacac gatcaatcct tgtaaataag cgaaaaatgg gccgtaaacg
    10201 gcattcacga tcctgccact ttgctggaat ccataaaggg aaggaaaata atggaaatcc
    10261 ccatatttaa tttgttgcgc ggcatcatag aaccgattgt aatggaatgg ggcatcagca
    10321 cccaaaatta gtttatgtgt gaataaatga ggcatgagaa atgccaaagc aatgaaaaaa
    10381 attgctaaaa atgggccata ttttttcaat tgtcgaactc ctttgcaaac tttagataaa
    10441 ttcatcatat atcctatgcg agaggtaggc aagaaattag aaaattcatg aaaatattta
    10501 tgaattttac aagaaaaaca taagtgaaag ttctaaggag agaggatgcg acataagttt
    10561 tgttgcatct ccttgttttg aaacagaatt tttcttaata ctcgaaacaa agtagctttt
    10621 tcatgacctc tcggtaaacg gtgttcaatc agctgcttct cggtaataag ctcactagcc
    10681 tagaaaatat gagaagctat tttctgacta gcgctcttat tactcaaagc ataacgcagt
    10741 tttcacaacc tcttccttaa acggtgttca aaagcttgca tctgcggtaa taagcccaac
    10801 ccaacaaaaa atatgagtag ctattttttt gggctgtact tattacttga tgcaggacag
    10861 ctttttcaca acctcatcgg ctttcatttt ctattttttt cctgaaccca tataataaaa
    10921 gaaagaacaa aaaaacttgc aagggaggac acatgtgaag cagagtaagc aaaaacagtc
    10981 tcgtcgactt ttgtttcgtt tgtttttatt aatatgtatc gtcgtagggt tactgatcgc
    11041 tttatatcca ttttatgtgg attctttaaa tagttttatt gatcaaaaac gtatcgaaca
    11101 agtacaaaaa cggaccgcag ctgaaattga agtgcagcga aagaaaatgg aggagaaaaa
    11161 ccaacgatta actgaccaag gattcaatcc tggtactgat cctttcaatg aaaagaacag
    11221 aaatgaaagt acaacatcca gtcaattaga ggaatggctg atcggaagta tcaatatacc
    11281 taagatccaa ttaaatattt cactttatga tcggctcaat ggtaggatcc ttgaaaatgg
    11341 agcaggcgtc ttgcaaggaa cctctttccc gcttggtgga aattcgacgc attcagtgat
    11401 ctctgctcac agcggtttac ctaatcgacg gttatttacc gaattagatc gtttagaaaa
    11461 cggagacacg tttattttga ctgttttggg ggagaaattg gcttatcaag ttaaaaatat
    11521 acaagtggta ctgccagatg acacgtctgt tctaacgatt tcagaaggag aagacttagt
    11581 taccttgctt acatgtacgc cgtatatgat caacacgcat cgtttgctag tgacaggaca
    11641 tcggattcct tataacgaat cggtaaaaaa agaagaagga aaaggaaatc aggaaagaac
    11701 catgcggcaa tggtttattc ttgcaggaac gataatagct gtagtcatac tgttagtgtt
    11761 tatcggacga ctaatctatc agtatagatt aagtaaaaaa gtgcttgatt tttcatttat
    11821 tattagtgat tcgtcaggga aaccggtaaa tggtagttca ttcattttga aacataagaa
    11881 aaaaacactt actagaaatg gtgtgccgtt ttcagtacaa agcgaccatc atgggaaagt
    11941 caagctggat caattgcctg gtggaactta tcgaatcgtt tcagctgatg atcctaaagt
    12001 tgctgtttct tttggtatta gaaaagtaaa acaagagaag atatactttt ttgaggggcg
    12061 aaaacttgta aaagagcttc aaaaaaatgg tttttatttt aaaatcaatg aataagtaag
    12121 gagagaaaaa atgatccgca caacaacaaa attgtctgcc aaagaactgg aatctgttct
    12181 atcgatttgg ctgcatgcta acttggattc tcatgaattc attccatcaa cttattggga
    12241 gaatcaagta gaatctgtta ggaaagcttt accagatgca gtgatttact tagcagaaat
    12301 agatcaagaa attattggtt ttgctggggt gattgatcag tttatcgcag gaatctttgt
    12361 gaaaagggaa taccggcaac aaagaatcgg tacgcaacta atgaaaaaaa taaaagaaac
    12421 caataaaaca ctgactttga cagtctatca aaaaaatcaa tcagctattc atttctatct
    12481 ttcacaaggt tttacaattc aaacacagca aaaagatgaa gcagtaaatg aggaggaact
    12541 tgtgatgaaa tggaatcaaa acgctgattt ttgatgaaca gaataacgaa ataatgctcc
    12601 cagcctaatt ttttgctggg agcattattt cattttgaag tcactaattt aactgtgtga
    12661 atatgcagtt gttacaatct ttgataaacg gtgttcaatc agctgaccct cggtattagc
    12721 tcaaaaaacg gcaaaatatg aggaactatt tttccatttg tttctctaat actcaggtca
    12781 aaacgctgat ttcacaacct cttgataaac ggtgttcaaa cggctgcttc tcggtaataa
    12841 gcccactagc ctagaaaata tgaaaagcta ttttctaact agcgttctta ttactcaaag
    12901 cataacgctg atttcacaac ctcttgataa acggtgttca atcagctgac cctcggtatt
    12961 agctcaaaaa acggcaaaac atgaggaact atttttccgt ttctttctct aatactcagg
    13021 tcagaacgct gatttcacaa cctctctttc acaaccttat tttactcgtt tataattttc
    13081 atcgaagtat tttccgctac ggatatcatc tggcattcca tcgtatggtt cttcgctaat
    13141 aacatcttca ttgtctatac ctgcatcacc catataagtg atcgtagcaa attcaggaac
    13201 aagtaatttt ggataagttt catatttttc acgagctgct tccatttctg cgatatgttc
    13261 gttttggaaa gtaacagtga tttctggatg ttcttcaacc catttaggga aaaagatcat
    13321 tccgtgaagt ttcttttcat ttggcataaa gtccaatgcc acgtctgtcc ctgttggctc
    13381 tgtccaagtg attttgaata cgccgtccgt caatttaacg atatcagctt cttggtcttt
    13441 tacccaacgg cctttgacca ttccgccatg gatacggtaa tcaacagtat ggtcattttt
    13501 ggcataccat tcatattccc aaccattgtc atacgtatag ataaagtgag ttccaataaa
    13561 atcttccaat tctttaaatt ctttcatgtt taagcatctc cttataaatt aaaacatgta
    13621 ataaactaca tgttttcgat tactcattta tgatagaatg gatttaaaga tttgtcaagg
    13681 agggaaaact gatggcaaga aaaaatacac ttcgatatat tttgttaggg ttattaagta
    13741 agaagaagat gacaggatat gaattgaatc aatcgttcaa aaatgaaata ggagaatttt
    13801 ggcaagcaaa acatagtcag atttatcctg aacttgcaaa aatggaagag caaggaatca
    13861 ttcagcatca agtagagatc acaggtgaaa aattggaaaa gaaagtttat gaattaacag
    13921 aagaagggaa ggaacaattg gcagagtgga ttcatacacc caccaatgaa ctaccggtta
    13981 atcgagacga atttgttctg aaattgtatt tcgtcaaaga cgtgaatgat ccggcattag
    14041 tagaaattat tcagcagcag cgtgaccttc atgaagaaaa acgacttcat ctattagagc
    14101 gaaaaaaaac gatctttcct actgaaaaag aaaaacatga aaattacgga cactacctga
    14161 ttttagcgca tgcgatcaat cgggaaacag aatatgcaaa ttggctaaca gatgtattag
    14221 aagaaagtaa ccgaaaaaaa taaaaaaata tccgaacagt gatggagaat tcctttctat
    14281 atgaaagaaa cgcagatcac cgttcggata ttcttatgtt caaacaggcc ttttttcaag
    14341 tatttatgta gcatctactt attaaaaagc ttggtactta ggagattagt ggcgtttaga
    14401 gttcttttaa caactgttcg tagtcagctt tttcatcgac aggaaggagc ttgtaagtta
    14461 attcgtctgt ctcgttgtca tgaatgatgt acatcttagc attctcttct ttgaagttac
    14521 cgatttgtaa aatcgtatag cgttcgtcga tcaattcgat atctttgatt gcttttgaaa
    14581 aaactttacg gacatggtgc attttctttt ggcggatttc tgccttttct gcagcttttg
    14641 acaaagtaaa tccgtcatct aaatattttt tgatcagttc tacttttatg actgtaggta
    14701 aacgatattt tctaggacaa ttcgcatcag cttcgatcga acgaatgaat cccttttgtt
    14761 cccaatacct taattgacgt ggagacacac ctacgatatc acttaattca ctgatgccaa
    14821 caaccaattg atcattttcc aacaaacgtt taagggaagc cccaaccatt tttgtcactc
    14881 cttttgtcct cttgcttccc ttaattatat ccgtattaac ttatgtgtca agtaggttga
    14941 caaatagcga atttaggact aaagtattct agtagtttca gaaaaaattc agaaagaagg
    15001 agaaatatga gtaacaacca acccgtagat atttacggaa aaccatataa tcggacactt
    15061 ttagttgttg tcttattaat tggtacattt tgtaccgttt tgaatcagac cttattgacg
    15121 acagcttttc ctacattgat gaaagctttc gatatttcag cgtctgatgt gcaatggtta
    15181 acgactggtt tcttgctagt caacgggatc atgatcccaa taacagcatg gctgatcaat
    15241 aaattcagtt caagaaattt atatctttct gccatgacga tctttttgat cggaacgatt
    15301 acttgcttta cagcacctaa ttttagtaca ttactaatcg gtcgtttgat ccaagcttgc
    15361 ggtgtgggag tatcgatgcc attattgcaa aatatcatgc tatctatttt cccgccagaa
    15421 aaaagaggat cagcaatggg gatgtcaggg atcgttattg gacttgctcc tgctttaggc
    15481 cctacattat ctggttatat cattgataac taccattggc gtgacttgtt tggtatggta
    15541 ctgccaatcg ttgttcttgt cttggtttta gcattcttcc taatgaaaag tgttattcca
    15601 ttgtcaaatc ctagcattga tattttatcc gctattttat caacgattgg attcggtagt
    15661 ttactttacg gattttctag tgtaggagat gacggctggg gaagcttgaa agtaattggt
    15721 ttcttagttg ttggtgtgat tttcatcgcc ttgttctcat ggcgtcaatt acatttggaa
    15781 catccattct tggaacttcg tgtattcaaa tcatctacat tcacgattgc agctgtttta
    15841 gctggtgtaa caaatatggc gatggtaggg gcagaaatgg tcgtaccatt gtatattcaa
    15901 aatattcgtg gagaatcagc attccattct ggcttgatgt tgttgccggg tgcattgatc
    15961 atgggggcta tgatgcctgt gacgggtgcg attttcgata aatacggcgc gaaacgttta
    16021 gccatcacag ggatgttcat tttgacagcg gcaactgcac catttgcttt cttgacaaaa
    16081 acgactccag ttttatatat cgtgatcctt tatgcgatcc gtatgtttgg gatctccatg
    16141 gtcatgatgc cggtaacgac ttctggtatg aatgcattgc caaataactt gatcagtcat
    16201 gggacagcgg taaataatac attccgtcaa atcgcaagct cgattggtac agctattttg
    16261 atcagtgtgt tgacgaatgt gacaaaagca aatatcccaa gcaaatctgt attggataat
    16321 acgccattag cttataaaga tcaggcaatc aatgccacac tgtcaggtta ccatgccgcg
    16381 tttatcgtag cgatcatctt tgggattctt ggctttatta ttgccttttt attgaagaaa
    16441 aaagaacctg ttctaatgaa agcgggggat aatgcatgat tattggagtt ttgattgtca
    16501 gcttgcttgc ttttgcttta acgaacgtgt tcgctaaaaa aacatggcaa acctttttat
    16561 ctttgatttt cggactaatc ttcgtcgctt cactaagttt gatcgtcgct aatttgtcca
    16621 atcactttgg aatggaaaaa gtaacggaaa caaaaacaga aaaaattgtt tccagtgctg
    16681 attcccaagg agcagatatg cttttgtata aagctttagg gaatggaaaa gaaaaagtgt
    16741 atctttaccg aacaaatgaa aaacaagaaa aaccaaaagc aactggtacg gataacgaaa
    16801 caaataaagt agaaaagaca gacggcgatg ctgaaaaagt gacgaaaact acttattggg
    16861 aatacaaaaa cgacatgtat aaattctggt tcaatattgc tgataacaat cacgaatatg
    16921 acagccgagt gaatacattc aaaatcccag agacatgggt agaattaagt acggaccaag
    16981 cagccaagtt agctgaatta gtgaagaaac agcaaagtac gatggaaagt gaagcaaaag
    17041 cttatgttca agacggtatg gtaaaagcaa tgactgaaaa tccaaaaatg agcaaagcag
    17101 agcaagaaca acgtacgaaa gccttagctg ctgaattcca acaacaagca ttcgcaaaat
    17161 tagtgaaaga agcaaaaggc gaataatatt ttgaacacca tgtaggaggt tgtgaaaaag
    17221 ctgctctgca tcaagcagta agaacaatca gataaaaata gcactccata tttttcatct
    17281 gattgggctt agtgccgtag atgcaagctt ttgaacaccg tttaaggaag aggttgtgag
    17341 cctgccgttt aactctgagt cacaaggagc aatttcaaaa aacagcttct catgtttgtt
    17401 gactattgtg acttgtgacc gaggagttgg caggagaaca ccgtttattc gaaaaaagga
    17461 actgcgacaa gacttttgtc acagttcctt cttttttttg ctatgctata ctagatgtaa
    17521 aaaataggag aaataaaatg agggaaattt attcttttga atcaaaaatc tatgcttccg
    17581 aagtcggcaa gggcggggca tatattattt ttccgtatga tataagaaaa gaattcggtg
    17641 ctggaagagt taaagtccaa gcaacttttg acaatgtgcc gtatgaaggc agtatcgtga
    17701 atatgggcgt gaaaaatcca gatggttcag tctgctacat ccttggacta cgaaaagata
    17761 ttcgtaaaaa catcggaaaa gaaatcggtg atattgtagc agttacagtg aagcaaaaat
    17821 aaaagaggtt gtgacaaaag acgtgtcaca acctcttgat aaacggtgtt caaaagccag
    17881 tttctcggta gtaagtcaaa tttatgcaaa aatgtggaaa cccatgttta caaaattttt
    17941 cttactactc gaaacgaagc ggctttatca caacctcttg ataaacggcg ttcaatcagc
    18001 tgtccctcgg tattagctca aaaacggcaa aatatgagaa gctatttttc catttttttc
    18061 tctcatactc aggtcataac gctgatttca caacctcttc tttcataatg gtttttcacc
    18121 atctttataa taaactggtt ctttgatatg gaaatctatt ttttccaacc aatctaaaat
    18181 cttgtaacct aactcttctg acatcggaaa agaggcgttg atttgtgcat tggagatatg
    18241 gtcaacatgg ataccggaag tgatcgtaca acttcccgga acggcagttt tgatgatcac
    18301 agctattttt tcggctaaga catcgtcttt atggaaccga ccatcgtggc tagggaaacg
    18361 tacagtttgc atctctgtat cacgagaaaa tgtagtgact gttccgatat gtggtgtatc
    18421 tccgcctgtt aaagtgatca ataagtcttt accgattact ttaacagcag cttggatcgt
    18481 gaaacctagt ttggttactt caactatcgt ttctttttcc atattaatca ccctgccaac
    18541 gttttgaatg ctcatttaca atttcaaatt gatccaaaag tttcattgta tggtgattaa
    18601 caagatcttg gatcgtttga ggctgattat aaaaagcagg aattggtggt atgatttgca
    18661 cacctagtcg tgctaattta gtcaagtttt ccaagtgaat ggcatttaat ggcgtttcac
    18721 gaggaacaat gactaacttt cgttgctcct tcattgttac atcagcagca cgtgcaatca
    18781 ggttgtcgcc taaaccaaca gagatacctg cgactgtttt catacttgcc ggtacgatga
    18841 tcattccatc ggtcaaaaat gatccgctgg cgacagcagc acctaaatct ttgtttgaat
    18901 aatggtaatc aaacaggctt tcgaattcag ttagtgataa gtcggattcc agctttaagt
    18961 tctgttttgc ccatggactg atgattccgt gtgtttctat catcggatat tcttttagtt
    19021 tcttcactag gttgatggca taaatggtgc ctgaagcgcc acttacacca atcacgattt
    19081 ttttcttccg ttgtgccaat tgttccattt tccgcactcc tttttatgat gtcaaaacag
    19141 ttgcagatca ttcattattt caaatatttt tcccaatctt ctacttcttg gaattttgca
    19201 cgtacaaaat gttctttcat atcgaaagga actgtaccgt caaagatagt ttttgcactt
    19261 atcccacgga aacgaataga gccaggatta tattctggta attctgatgg gtccaatgga
    19321 tggttcctca ttcctgggat cacgatgata tctttatctc cttggaaacg agtgttgaaa
    19381 gtccaaacta catcgttcat gtcgaaaata tcaacatctt catcgactaa aatgactgtc
    19441 ttcaattctt tgaaggctga taaagcaaga atagctgctt gacgctgaat accttcatcc
    19501 gcttcgtttt ctttacggat ttgcatgatg gtcatcaatt taccaccacc tgcaggagga
    19561 ttgtagacat ttactacttt tcctggaata gctttatcga ccaaggcaag aatacttgct
    19621 tctgtaggaa taccggccat agatacgtgt tcttcactgg gaccaatcgt tgtttgcatg
    19681 attggattgt cttttcggtg agtaactgct ttgactttca ctacttgtac agctggatta
    19741 gcatccccag tatagccagg aaattctggc attgcttttc ctgagttcgt gttgatatct
    19801 tcttgcattg tttcatttgg catgatttct gcttcaatga tgaattcagc acgagcaata
    19861 cccacttcat cgactgctat accatcaacg atttgaatag gttcgttacg caaagctcct
    19921 gcacaccata gttcattgta gccaagtgga gtggtaggtg gttcgaaagt cgtgccaatg
    19981 gcaattgctg gatctaaacc aatattgata gtgataggca ttggtttatt caaggcttcg
    20041 aattgttttt ggaagtgacc aatgtggcgt ccgccaggca tgatgtacat accgatcgta
    20101 tctttatctt ctagaaccat acgatggatc gttacatcgc tcattgtttt ttctggatct
    20161 gatcctaaga ctacacccat tgtgatgtat ggaccagcat cttcaggagt atttgtaggt
    20221 gcaggcaaaa tgttgcgaat atcgaagtct gcatcggtag ctagatgaac gacttcttgt
    20281 gctggagcgt ctgctttgtc tactttcaca ggtttgattg gattttctac agcgtctttc
    20341 aagaaacgac caagtgttcg ataatcatgg tgcaaaattt cacctacacg tttacgacta
    20401 gccattgttc cgatgttcac acgtgtgccg gggaaacctt tgatattgtt gaaagtcata
    20461 gctggtcctt cttgagtcgg acgttttact gtaccgccag caccgatata acggtaaaca
    20521 ccagataatt ctgcttcagg atcgatttca atatctgttt cgtgatattg acctggaagt
    20581 gtttttagtt cttcgatgac ttttcttaaa tcatacggtt gatctgtcat gagagattcc
    20641 tccttagttg tctttaccta tctatagtaa aaggtgaaaa agaggaaaac aattcaactt
    20701 gttcggtaaa gtattctaca atggaatgga tgcgtttttt tgataataga ataaactatg
    20761 atatacttat cactaaaaga aagcgcatac ttagtgttgg aggaaggaaa gtgaaaaaaa
    20821 tggaggagtc atttcctaaa gcagtaaaag tggaaaatat agcgaatatt ttgaaagtta
    20881 catttgaaaa cggagaagta aaatacgtga aatctcattg gacagaagag ataaccgatg
    20941 ccttgcaatt tggaaaaaaa ggaagaggaa aaagaaaaaa tcttctcgca ctttctcgta
    21001 atatgtggat tgggactgaa gtaacgattg aagcagatgg aacagtattc ataaatggaa
    21061 aagacaggta tacgccagaa gagttgtggt acaaaggaaa gaaaagtata ccagaattat
    21121 aaaaaataat taaaaaaaac agcgcgaaac aaggaacata ttacttcctc gtttcttgct
    21181 gttttctact atttcagcgc tcttatgact caaatctaaa tgtggttgtc acaaccttat
    21241 tctgcacgca aagcgatagc ggcgtctttt ttcgctgcca ttcgtgcagg aagatgaccg
    21301 ccaagcatcg tcaatatagt agagacgatc actagaataa tcgcatggac aggatttagt
    21361 tgagcaacat tttttagatc cgtcatgttg tataagatgc tattgatagg gaacgtagct
    21421 agccatgcaa taaacacacc taatatccca gaagaaatac ctaaaataca cgtttctgcg
    21481 tcaaatacgc gagtgatgtc ttttttacgt gcacctaaag cttttagtac accgatttct
    21541 tttgtccgtt caatcacaga tgtataagta atgatactga tcatgatcat acttgttact
    21601 aaagagatcc ctgcaaaagc aataaggaca taagtgatag catccatcaa tccgccagtc
    21661 agttcagtca tcgttccagc taaatcagta taaatgattt tatcttcatc tgatttgcct
    21721 ttattgtaat catctagata atccaagatc ttttctttgt cttcaaaatt gttcggataa
    21781 atcatgatac ttgaaggcaa gctgtctcca cctaaataac taagcaatgt ttgtttggca
    21841 ttttcgtcaa ctttttcagt agttaagaca tttacaccac tgtctctttg agctttgacg
    21901 atttctgatt ctttgttttc gttgacgatt tgagtcgtta attgatcgct ataagcgatt
    21961 cctggagata agagattcat tgtagaagaa gatttcacgc gtaggatccc cgagatcttc
    22021 aattcatcag aagcattctg atacatcgca tcataatctg tattagggat aaaattccca
    22081 gttggaagtt ttgtatagta agtattgttg tttactaatt tgaatgttgt accaacgata
    22141 tcatcaaaat caagtttttc gtcttctttc acatcaaatc caagattctt caatgcgttg
    22201 atattagtat tattattgcc gtctacgatc agcacgacat ctgttgctga ggctggataa
    22261 cttccggcaa gtaaagaata attgtctttt aaaaagtttt ctttgctagt gtcaagctgt
    22321 gtagggaaac ttgacactcc cacacctgtc atcgctgaca tcgtactaga taaagaaaga
    22381 gattccgtat caggattttg attcgagaag ctgacaggct gtactttgcc atttacatca
    22441 cgtaaaagat tgattcctgt agtacgggta aatccgatat tattacttaa gttaggatcg
    22501 atgtctgtta cgtaatcgac gtatgtttga tcaatcttat tcgtgtgctg tgctttatct
    22561 tcttcgctga cttttgcagt aatcgttttg ctgtctggat aatccgcttt gcttgcaccc
    22621 aatccggcat cgtcacgagt ctggttagtc gttaccttag aaatagtaat cggaaatttc
    22681 gccatcgttt cagcttgcgt gttatcgatt tgtttttgga atccagaaga aagggacaga
    22741 acgatcgcga ttccgatgat cccaatactg gaagcaaagg aggtcaagaa agtacgtcct
    22801 ttttttgttc ggatattgtt gaaggaaagc tttaacgctg tccaaaaact catctttgtt
    22861 ctgcgtaagt taaattgatc atcttttggt cgttcgatgt gaggatggga atctgttaag
    22921 atcttaccat cagaaaattc aatgattcga tctgcgtatt gatgggccag ttcaggatta
    22981 tgtgtgacca tgatcactaa tttttctttc gataactctt ggatcaattc catgatctgg
    23041 atacttgttt ctgtatctaa cgctccggtt ggttcatcgc acaaaaggat atctgggtca
    23101 ttggctagtg ctcgagcgat ggccactcgc tgcatttgtc caccagataa ctgatttggc
    23161 tttttatgca tatgatttgt caagccgact cggtgcaatg catcttctgc ccgttttcgt
    23221 ttttcgtctt tagaaacacc acttaatgtc atcccaagtt caacgttttc aataatgcct
    23281 agatgaccaa tcaagttgta actttgaaaa atgaaaccga tagagttgtt tcggtaggca
    23341 tcccagtcac tgtccttaaa gtctttagta gactttccgt tgattaccat atcaccggaa
    23401 tcataattat ctagtccacc aatcacattc agcatagtcg ttttaccaga accactagga
    23461 cctaaaatcg ctacgaattc tttttgtctg aaagcgacag acacaccatc caatgctttg
    23521 gtggtggttt cgccgacttt ataatatttt tttatatctt ttaattgaag cataatcacc
    23581 cttctcccgt attgaaatat tcggtatact aataagtata tcagaacaat gatcgtagta
    23641 tacgaaataa atgttaactg aagatgaact acaagaaaaa atctagaaat ttttcaaaaa
    23701 acagagggag atcaatgaat cgtattttag tagtagaaga tgatccgcaa ttacagttgc
    23761 tttaccgttc tgtgttggaa cgagcgggct tttcagtcat caccgcattg aatggagagg
    23821 atgcgctgaa aaaactagaa gcgacacgta tagaattaat cattacagat atcatgatgc
    23881 cgaaaatgga tggctatgac ttgctagact cattacgtag caccagattt gacacacctg
    23941 ttctaatgat tacggcaaag gccgattttg aggataagaa acgcgggttc aaattaggag
    24001 cagatgatta tatgacaaaa cccatcgatg tcaatgagat gatcctacgt gtggaagcat
    24061 tgcttcgacg ttcaaagatc aattacagca gtagcttgat aatcggacaa acaaaattag
    24121 agctagatgc ttatacagtg ttacaagatc agcattcgtt gattctgcct caaaaagaat
    24181 ttctgcttct gtataaatta ctgtcttatc ccaacaagat cttcactcgc caacagttga
    24241 tggatgatat ttggggatta gatacgaata cagaagaacg aacgatcgat gtacatatca
    24301 aacgtttaag gacccgtttt gaaaagacgg aagattttca aatcatcaca gtacggggac
    24361 taggctataa ggcggttatt taaagatgaa aaaaaagcta ttttcacaat tatggatcta
    24421 ttttgctagt attgtcttca tttcgatctt agtgacactg ctgtgttttc tcggttttat
    24481 tttcttattg tctcgacatt ctatctcgcc tgctgaatcg gctggaaaac caacgttata
    24541 cccgttattc gtctttgcgg ggttcagtat ggtcgtgggt acaggaatat cgatttttgt
    24601 tgggcggcgt atcctgcatc caatcagtgc gctagggaca aatatgagtc tggtagcaac
    24661 gggagatttt tcgattcgaa tggatgaaca gcagaaagta gcagaagtac agcaactgta
    24721 taaagatttc aacgttatgg tccaagaact caatagtatc gagacactga ggaatgactt
    24781 tgtgtccagt gtatcccatg aattcaaaac accactagca acaatccaag gatatgttca
    24841 attgttacaa gccccaaatc tctctgacga agaacgccaa atatttttat atcggattat
    24901 cgaaagtatc acgcagcttt cacaattaac agagaacatc ttaaaattga acaaactgga
    24961 aaaccagcgt atccaactgg aaaagaaaga gtatcgatta gatgaacaaa tcagagaagt
    25021 aatcgttttt tttcagccta aatgggagaa agaacagcta gaattggata tcgaacttgc
    25081 tgcagtaaat tacacaggca atgaagagtt tttgtaccaa gtgtggctga atatcatgga
    25141 taacgccata aagtacaatc aaatcaatgg acagattcat atcaaactat ttgagacagc
    25201 cacagagatt gtactggaag tgaccgattc aggtgtagga atgaatgaag agaccagaga
    25261 tcgaatgttt gaaaaatttt atcaaggaga tacaagtcgt caaatttctg ggaatgggct
    25321 aggactttca ctggttaaaa aaattctaga actgcatgat ggacgtattg attatagcag
    25381 tatcgaagga gtaggaacga cagcaatgat acggttaagt aaacaatagg aaaaagaata
    25441 aaacccctaa aaattctatt gaaggttttt aggggttttt agtcgttttt ttgtgattag
    25501 ggaaatgttc ttacggttct tttggcataa aaatcgctgc tttcttattg aactacgaga
    25561 aatgttagag gtgtcattga aacaaggaca tgatgcaaga aagagagcga aagtcatgcg
    25621 tcgctctctt tttttgggtg gatagatgga cagaaaatgt agggcttagt tttcttcttg
    25681 atttaaatac tcccaaaatg tatctgaagc caaagaatgc tcaccgactt tacgagtaaa
    25741 tgcaagataa ctctctgttg ttggagaaat agggagaatc acagtatttt cttttaatag
    25801 attcttgact gttttttcca taacaatagc aatccccatg tcatttgcaa tcatattgat
    25861 gatcaagtcg attcttgctc ctttataaga gatttttggt tcaaatccag cttcgcgaca
    25921 cagatctagt acaggttgat acaagttcgt agtggggcca aggagcaaaa agttctcctt
    25981 tttcagttcg cttagatcca atttcttttt actagctaaa ggatgatttt taggtagaac
    26041 tgctacaaaa tcgtctgttt cagtaggcaa ccactcaatc gaacttggtg gggcctggaa
    26101 acttctagca aagtggatcg tgtcactatg ttcattggcg tcattgtaag ttaattcgac
    26161 actttccact tctttcaact ggactgtaaa ttcaggatgg cgtttcaaaa actcggtgat
    26221 tttggaaaaa cctttataat tgatcattgt cggtatcgtt aagatattta atgaaagatt
    26281 gttgtctttc gtataggttt caagcacttc ttgcatagca tggtattgtt ctacgacatt
    26341 ttttacatat gggagcaata agcttccagc ttcggtcaat tcgatttttc tatgggcgcg
    26401 ttcgaataat ttgacgtcta attctgtttc taacgaaaga atctgtttag aaatatttcc
    26461 ttgggtggtg aattgtcgat cagcagtttt ggtgaaattc attgtttcgg ctaaatcaag
    26521 aaaaacttcg aatttatgga tatccatcat attcctccta cttattccaa taaagaatac
    26581 tttaacacaa aaaatgaatt ggtgtaatct tctctgtctg ttacaatctt tcttgcagcg
    26641 aaagcaataa ttacagaaat ttttctgatt tttttcgctt cgtagaaaaa gagaggagtt
    26701 ttttgatgcc aactggagtt ttgatcaatg taggttccgt tttgtttggc ggactaattg
    26761 gcggattagt aggaaataaa ttaagtgaac attttaaagc acagctgacg atggtttttg
    26821 gtgtgtgttc gatggggatg ggaatctatt ccattgcgcc gatgaaaaat atgccggctg
    26881 ttatttttgc tttagtgata ggaacagcga ttggtttgat tgttcattta ggtaatggga
    26941 taaataaggg ggctgcgctg atgcaagtac ctatttctaa aatcttccct agtgaaaagc
    27001 ttggtatgac ccacgatgaa tttatcagca cattagtgac ggtcattgtt ctattttgtg
    27061 caagtggtac ggggatctat ggaagtttag attctgggat gacaggagac agtacgattt
    27121 tgatttctaa atctgtcctt gattttttca cagcagctat ttttgcgtgt aatttagggt
    27181 atgtcgtttc tgtgatagca attccccaat tcattatttt ttatattttg ttcttattgg
    27241 caaaatttat ttatccattg actacaccag acatgatctt agattttaaa gcttgcggcg
    27301 gattcttgat ggtcgctacc ggttttagaa tgatcaacgt gaaaatgttc ccagtagctg
    27361 acatgatacc tgcaatgatc gttatcatgc cactgagctg gatgtggaca aattggatta
    27421 tgccactgtt ataataaaaa tcaaagagaa gccatttttt gctgactaaa aaggatcaaa
    27481 tcttttagtc agtttttttg tatttgacaa actgattttg aaactatatt atatgttgta
    27541 taatatagat tgaaataaaa tattacactt gagaatagaa ggtgacgtaa tgcacatcca
    27601 gattccaact gttcttcttg atggtactgt gttagcagca ttaagcaagg aagatatgta
    27661 tgggtatgcc ttaacaaaaa tcgtacaaga aagtctctct gtcagtgaat ctacactata
    27721 tccggttttg agaaggttga aaaaagaagg atatcttgag acctatgatg aaccttttga
    27781 aggcagaaac cgtcgatatt actcgttgac accaatggga agtcgtcatt tagaagaaat
    27841 tattgatgaa tggattgttt ttcgagattc tttagaccgg ttattggagg agagcacaaa
    27901 tgaatgaata tttgaatgaa gttgtggatc atcttcaaga aattcctgaa gacgatcgtt
    27961 ttgatttgat ccagtattat gaggaatatt ttttagattc tggaaaaaca ttagatcaga
    28021 tcattgatga atatggtaca ccaaagcagt ttgccttaaa attaaaaatc agttactttt
    28081 cagaagaaga acaagcagaa aacgagttag tgaaaaatcc aaaaagacaa attcgcctga
    28141 tttggttaat cattatcggt ttatttgctt ctcctgtcct cattccacta gcactggggt
    28201 ttatattggt tattggggga ctattggttg ctttactagc gctcattttt agtatctatg
    28261 ttttatgtat cagtatctta ggtgttggcc tattcatgat catctcagga atcagtgtgt
    28321 ttggacaatc gattctgagt ggattattct ttatcggtgt cggtctgttc gccacaggaa
    28381 gcgcagtatt tatttcgcca ctgttgctga aaggaacaaa agggcttttt tatgtcatga
    28441 tgaactttgt taaatggatg ggacgacgtt ttattacaaa aagagatctg cattcagcgg
    28501 gcgtttgaag gaggacacat aaattgagat tgaaacattt tttgattacc ggtattggtc
    28561 tgatgcttac tggtggcctc ctagcaggtg caacttatac attaggcgct caaaagagtc
    28621 tgacttggga taatggtcct cggttgatcg atatggttac tgagacaaaa aaaatcaagg
    28681 atgcaaacaa aatcgtcatc catgcagaaa atcaaacagt ttcggtcata cgcggattgg
    28741 attttgagat aaaaacgact tatgataaga cagaaaagcc agaaataaag gagaaaaatg
    28801 gcgtgttgac tgtgaatgca gtcaaaaaat caacaggtgc aatcattagt ttagaagaca
    28861 caagtgcaca aatcactatt gttgtccctg cagatcatga attatctgaa ttgacaatcg
    28921 aagggaataa tagtgcaatt gatatcaacg aagtatcaat caaaaaagtg aatactaaga
    28981 cacatgatgg ctgggtatcg ttcaatgaat ttacaggagg aacaataact gcgaaagatg
    29041 aattaggctc acttcgacta gatgaggcca atgtggataa cttgatcgtc acaggtgaaa
    29101 acagcaactt ccatctagaa gacagtttga tcagcaaaga aggaaaagtc atcctaaacg
    29161 gtggtaattt atctttgcat gagatggaaa acagtggcta tactatccaa acgtctggtg
    29221 aatcttatat tgaaaaagac taccagccaa taacaaatac tttgttagaa caaggacaga
    29281 aaaagctaaa gatcactgct aaagacacat cgatcaatat tacgacggat caagaggaca
    29341 atgaaacaga ttgatcctgt actcaactgg atattttaag aaaaaacagc aaagatcctt
    29401 ccaatctttg ctgtttttca tcgcttaacg cacataacta tagtaaaata agaaagaaaa
    29461 tatctaaaaa gaaaacaggg tgaaagagta tatgaaggta gaagaaggtc aaagagaaaa
    29521 gttaaagaca gaaatggaaa gattacatac ttatatcact caactaagcc aaaccttcta
    29581 tgatccagac aaagaaaaag tgatggttaa ttacccgaac aattcagaag gtcgccaatt
    29641 agagcaagtg taccatgaag ttttcaaaca tttattgacg gtgaagaaag agctggatta
    29701 ttattcttta ccgattatcg atacaggaat attgaagtat gatgggaaga aggaacgatt
    29761 tatctttaaa tctgttcggg aagatttgcc tctttcagca gggatggatc tagaggtctt
    29821 ggtagaagac tattttacag aagaaaagca ctgggttcga acaaaattgg attatttacc
    29881 gcaggcagcc ggagggacac aggcaagcgg ttggtacatc actgaagaca aagagttaga
    29941 actagaaggt gtaatggcgc gtattcgaaa aaaaaattaa aaaagtaaca ttagatattt
    30001 attgaaggat aaaggagcgc aggaagtagc atgagagaaa aaagagcatt ttatatcaat
    30061 ggttatgcag ggattgtcgg attggtcgta ttagctttag ttggattgtt tttcttctat
    30121 ttaggtatgt ggcaagcgaa agtttgggca ctttttttaa gtatttttct ttggttgatc
    30181 acgttattgt tgttaagttc ggcaactgta gtcagtccta atcaagcaaa agtaattttg
    30241 ttttttggac aatatttagg tacgattcga gaaaatggct ttttccttac gatacctttg
    30301 gcacaaaaaa tgactgtttc gttgaaagtt cggaatttca atagttctgt tttaaaagtc
    30361 aatgatttgg atggaaaccc catcgaaatc tcagcagtag tagtctttaa agtcgttgat
    30421 acagcaaagg cattattcga tgtagcgtat tatcaagatt ttgtagaaat ccaaagtgag
    30481 acagccattc ggcatatcgc tagccaatac ccgtatgata cattcaatga gaatgatttg
    30541 acgcttcgag gaaacacgac ggcagtgtct gacgagttgc aaaaagagct gcaagagcgt
    30601 ttagcagtag caggtgtcga agtaatcgaa acacgcttga accatttagc ctatgcaaca
    30661 gaaatcgcta gtgccatgct ccaacgtcaa caagcaaaag ccattttgtc tgctcgacaa
    30721 accattgttg aaggagctgt cacaatcact caaatggcct tagagcaaat cgaagatgga
    30781 caagaaatca atttcaccga tgatcggaaa gtccaattga tcaataattt acttgtctca
    30841 atcattacag acaaaggaac gcaacctgtt atcaatacag gcgacatgaa agaccgtaag
    30901 tgacatactt tttgtaagat aggaagaagg agtttaggac ggattattgt cttgaactct
    30961 ttttttagtt gttttaaaga aaatgacaaa attgaaagaa gaatgatagt tgattcgaag
    31021 gcagaattgt tcgctttttt ctataatcag tatataaagt aagagagaag gggaaaaagt
    31081 gaaaaaactt ctgatcggtc tacttgtttt catcgttgtg gtacttgcag gggtgaaaat
    31141 cgcagattac gtagttatgg gtggagaaga atactatgta caaattacga cagatggtac
    31201 aaaaacagtt tcaaaatcag actctggaca agaaacagtc aactatagat atatcctgcc
    31261 tggctatacg aaagagggta aggaaaaaga actggacttc aagggacaat tgactcgacc
    31321 gcttcgtaaa ggagcatatt taaaaatcac ctggaacaaa aataaaggtg tcacttcgta
    31381 tgaagaagtc gaaaaaaaag acgtgccgaa aacagcactg gaaaaactaa ttaaagggta
    31441 gggaaagaaa tgaacaaaat agtcgaagta cagaatgtaa caaaagtata cggaaaaaac
    31501 aatgagaaaa agacgcaagc attaagcggc atcagcttcg atgtagaaaa aggcgaattt
    31561 attgggatca tgggcgcttc tggttctggt aagtcgacac ttttgaatat tttatccaca
    31621 ttagataaac caacagatgg gcatatccga atcaatcaaa aagatgtgac cacattaaaa
    31681 ggaaatcaac tagcagattt tcgtgcgaat gagattggct ttattttcca agatttcaat
    31741 ttattagaaa gcttgacagc acaagaaaat atcgctgttc ctctttcttt acaaggcgtc
    31801 agaccaaaag aaatcaagca acgtgtccaa aaaattgcag aacgtttatc catctctcat
    31861 attttggaaa gctatccttc tgagatttcc ggtggacaaa aacaacgggt cgccgccgca
    31921 cgtgcattga tcacacaacc aacgattcta ttaggggatg agcctacagg tgcgctggat
    31981 tccaaaagtg cccgggatct tttagatacg atggacgaat tgaatacaaa agatcatgta
    32041 tcgattcttc ttgtgacgca tgatccgttt tctgcaagtt attgccaacg tattttattc
    32101 atcaaggatg gcggtatcca tcaagaagtg aaacgtggag atcaaagccg tgattccttc
    32161 tatcgagaaa tactatctat tcttggaaac ttggaacagt agggggagat tgactatgct
    32221 ttggaaatta tcacttactg ggatcaagag ccgtttaaga gactacttgg tgttattctc
    32281 aggattagtt atggcatctg ctatttttta catgtttgaa tcaatggcaa gcaacgaagc
    32341 ttttttgaaa agtaatacga tcgttgcggc tacagttatc attttccgat tcggttctgt
    32401 tttactaggg atcatcacat tcgtttatat tttatatgca aattcctttt tgatgacgat
    32461 gcggcaaaaa gactatgcaa tgtttatgat gctgggagca aaaggcagaa aaatcgcaca
    32521 gatgattttt tctgaaacat tcttagtagg gatcgcagct actttagtgg gttcagctgt
    32581 cggtgtagga ttgacaagta tcgtccaccg attattagtg gatcagttag atctcaatat
    32641 ttcgcatttt acacctttta gtattcacgg gttgttgatc actgttttgt tttttgctgt
    32701 tttgttttta ttagctgctg ttgtcaatgc tttttcgatt gtcaaaaaac cgattttagc
    32761 attgattcgt gcggatcaga caccaacaag aatcaagcag cataaattct tgtttttcct
    32821 tgaagtagta ctgggaattg cttttctagg ctgcggttac tatatgatga aacacgtggg
    32881 gacctatcaa gtctttggta taggggtagc cttagttacg atcgtattag gaacttattt
    32941 cattttccat tctgtgatca ttttcttttt atcattacta aagaaatcag aacagatttc
    33001 attgaaaaaa ttgaacaatt tcacattgtc acagttaaac tttcgtattc gtgaatatac
    33061 acaaatgctc tcaatggtcg ctatgctgtt tgccttggca ctgggcgctt tgacggtagg
    33121 attaggtttt aaaaatgaaa ttgcagactt gacagaaaac aattctggat atgatctagt
    33181 gatgaataat cctcgggcag aagatcagca aaaaatcaaa gaactttcac caacactttc
    33241 tgcttcttat actcaaaaag aagatgcggg aacgatttac tataatgcaa gtgaatttgc
    33301 ggctcaacca ttgatcatga taaaacatga agatggaaca ccgccgaaga tcacgaaaga
    33361 aaaagttcct gctaaaaaaa tgaatgaact gactgtacaa gaggaacttc gaagctatga
    33421 attgccggaa cagcaagaaa aagaaatcaa ggtagtcagt caatcagaat ttgatcaact
    33481 gaacttgcca caatccactt tacagctagt tcaggtaaaa gacttccaag caaacctgaa
    33541 accaatcgaa acacttgttg ctcaaaataa aatgaacaat ccatctttgc aaaaagtaga
    33601 gtacagcaac cagaaatatg aaatgtatga agtgtataat ggtctattct ctggttttga
    33661 atttatggga ttcttcctag gtttagcttt cttgaccatg cttgctagct gtttgatgtt
    33721 caagatcttg tcaggaagca agagtgacat tgctcgttat gaaatgcttg aaaagatagg
    33781 aacacgacgc gggttgctta aacaatcgat ccgtagagaa gtaggcgtgt tattcttagc
    33841 accaggaatc ttaggtgtag tccatgtctt attcggatta caaatgtttg gtctattcat
    33901 gcaaaatcca tacaaagata tttgggttcc gtttacgata ttcttcgtat tgtatttcat
    33961 ctactatgta ctgacgacat ggttatatac tggaatcgta ttgaaaaaga gattgtgaaa
    34021 aagctgtcct gcatcaagta ataagaacag ccaagcaaaa atagctcctc atatttttcg
    34081 tttggttggg cttattaccg cagatgcaag cttttgaaca ccgtttgttc ggaaaaagag
    34141 gttgtgacaa tatttttgtc acaacctttt tttcacggtc ttttattaga atatagtaaa
    34201 ttttttctcg ttactcacgg aataagctgt taagaaaacg gtttccatgt atactggaat
    34261 cagtgcaaat gaaagaaaag agggtgcaag gaacatggaa atcaatgaac aaaattttgg
    34321 cgaaaactat cacttgatta cgatcacgaa tcaaaatggg acatctttgt ctgtcagtga
    34381 tcttggggca cgggttgttc attttcaaac aataatcgat aatgaaaaac gagagttgat
    34441 cttaggtttt gatgaagcaa aagaatatct agaaaaagac ccttatatcg gtgcttcaat
    34501 cggacgtatg gctggacgga ttacgaatgg cacattcaaa ttagatgatg aaacgttcga
    34561 agtaaaaaca gacccggaca ctggtcattg tttacatggt ggtgcaccta gctttgaagc
    34621 caagcagtgg cattatacag tcatcaatgg tgaaaacgaa gcatcggtca ttttctatca
    34681 gactagccca gatatggaaa acggatttcc tggaacttta gatgtagaag tacggtatac
    34741 gctgacgaac gatgatacat ggcgagtgac ggtccaaggg atcagtgacc gcaaaacagt
    34801 attcaatcct acaaatcatg tctatttcaa tttaaccggt gatccagcac agtcaatcga
    34861 cgaacatata ctttggttga atagccgatt atttgctacg ctcaataaag atacaacgcc
    34921 tacaggagaa gtagtttcag taagtggcac agcatttgac ttccaacgtc ctaaaaaatt
    34981 aggtgaggtc tttgccagtg attttcgcca atcaatgata gtgggcggca tcgatcatcc
    35041 atttttgttg aaaaaacaat ctggtttact aaatgctgcg gaattgacaa gcccagatga
    35101 aaaagtatcg atccaagtaa aaacagatgc ttcaggaatc gtgattttta cagcaaattt
    35161 tggagaagat ggaccggaaa tgcgcgggaa taaattagta aatcatggcg gtatcacatt
    35221 tgagactcaa gaactgccag gagcggagcg acttgaaagt tttggagatg tcactctgga
    35281 acctaaccaa gtacgcgagt ttgtaacgga atacaaaata aatataaaga aatagaataa
    35341 attaaaaagg gagtgtgaca gtgtcacatc ccctttttaa tttattctat ttctttaaag
    35401 gcagatcaag ccagaatgat ttgtctaaac ttaacaaggt tagtacagga acagttgcag
    35461 ctttaactgt acgagtagtt gtttctggta tctgtcttgc cgtaaaattg ttccacaaat
    35521 cccagattga agcgacacgg aagatggaag aagcgttgtt gacgcgtttt atcgttgaaa
    35581 attctaatcc tgtgattggc acgccatctg ttgtccattt gattggcaca tcgaatcgct
    35641 tagacatatt gaggatttca gcgtttgctt cttcagttgt tgctgcttct tttttcggtt
    35701 catcatattt tgtcaggtcg tacgcttcga tgatcgcgtt caatttttgt gaatattggc
    35761 gatctgtagc ataacgtcct tccaaatatt tggttgcttc tttgtagtct tttgtttccg
    35821 acttccaagt tggtttgtag aaatcaggat tgccagtcag tccatttttg atcaagtcgg
    35881 catagtcttc caaactttcc ttgtaagaag gatatttacg gaattgtgcg cggatttggt
    35941 agctttgtcc ggaactatct tgttcaagtg tattgaaggt cacgctgctt cctttatatg
    36001 atcctttgat accaaaaagg ttatgatttg gttcgcttga taataagctg tttccagatc
    36061 cactttcaag gatagcttga gccatcatga ctgatgcgta gattccttct ttttgcccga
    36121 tttcctgtgc atcatctgca atctttttca agaactgatc agttgtttgg ttttgactga
    36181 aatggaacga gccatctaat ccatctaaaa tagatgagcc gcctgtcagc gaacctgaag
    36241 cagcaggtaa acctgctaag tttactcgga cagcaaaatc aggcgtgaaa tctgaaatag
    36301 aagttactcg aactttatct cctggttgag gcgcatggat aaattgtccg ttcccaatat
    36361 agatacctac atggtgagta acaccggaag aaggtcccca gaagacgagg tcgccaggtt
    36421 gggcctgact tactggaatc tgcgtacctg catattgctg ttcgcctgtc cagccaccga
    36481 tataatgacc ggttgctttc atatatacgt aataagtcaa accagaacag tcaaaagtat
    36541 ttggaccttt tgctccccat acatatggtt ttccaagatg cttgaatgct tctcgaacga
    36601 ttgcttgttg gacattcgtt gtatttgtag gcaatacagc atcaattggt gattttacaa
    36661 caaaatcaga agaagcagaa gatggattct ctataccgct ttgatttgct gtatttggac
    36721 gagatgaagc attgttcgtt ccgccattcg atgaaccatt gtttggatgg tttggttctc
    36781 tctgagtatt tccattgtta ttcgatccag atgggtttgt ttgattcgaa gaattcccgc
    36841 tgttcgaatt attgttatta ttgtttgaat tatttgaacc attattattg ttgttattac
    36901 ccacatctgg tttgtttggc gtttccggat ctggtgtact agggacttct tcactaggat
    36961 cagttggttt ctctgttgaa gtatccggtc cagctttaaa ttctgcagta atagataaat
    37021 tgttcgtacc tactgtaaag gtctttgcat cataatttac ttctttggca ccattaacag
    37081 cgactgaagc aatttgttca ccttcagctg gcgtaatagt gtaagttacc ttcgtttccg
    37141 ctaataaacc ttctaccgtt ccactttccg taatatccaa aggttgctgt tcaggggaat
    37201 ccgctgtcga agcagtcagt tggatcttac caatcaaaga aggagcaatt gtaagggaat
    37261 atgtctcggc tggcttttca ttttctgatg gatcagccgg atcaatttca gttggctcgg
    37321 tcgatcctgt tgttgagtta gttgttccag ttgggtcttc tgtaaaagaa tccgttgttt
    37381 cactagtagc tgagctggat gtagtcgtat ctgttgtgac tgtttgatca gctatttgat
    37441 tatctacggc aaatacggta ttggtcgaca aaacagacgg agcaatcagc caagtagcag
    37501 ctagcaaacg tatcgttttt ttatttttca taataaacta gtccttttcc tttcattgat
    37561 tttctgtctt attcagcatt cattagacat tttttggaac agataatcgt gtcgaaaagc
    37621 tttttctttt cataaatctg tctgtgtatt ctaaataaaa caatttgtaa ataagcaacg
    37681 agcattcatt cgtttttctt tgacgttcat tttattgcat agaccacctt tgtttgggga
    37741 taaacaagtg gggataaaaa tggttatgca tatgtaaatt ataccaaagt ttggaaagta
    37801 tcacaaaaat ttataaaaat aatgatccta aaaagaaaaa gactgaaata tgtgataaac
    37861 atctcatact tcagtctttt tcattaacta ttcataagac attaggtctt catcaaaccc
    37921 gctaacgaga gtagtgatca ttgctacgac tagactaaca gccagtcctg ctaagtacca
    37981 gaaaaaagta ccggaaatga atgcaggcaa tgtggtcaga ctaccaaata caaaagcgat
    38041 cgctttcact tgggcaaatc ccattacagc tccaccagca gctgcaccaa tgcaagcagc
    38101 aacaaatggt tttttcaatg ggatcgcaat tccgtaaaca gttggttcgg tcactcccgc
    38161 aagtaaaatc gaagcaaaag cagaaccgga gatcgaacgg gtttttgaac gtttacttcg
    38221 cagaaataca cctaaagctg caccagccat tccaaaattg gctgctcctg tcaacggaac
    38281 gattcggtcg aacccataag cagagatatt actgatcatg acagggttta cagcccattg
    38341 gataccaaat attaccaaaa tattccaacc accagcgatg attgcgccac tgatcggtcc
    38401 gttaatatcc atcatccaag taattgcggc agctaaacct tcgccaaggt aaacacccgc
    38461 aggcccgaat accattaacg tcaaaggaac cgtgataact aatgaaagta aaggtacaaa
    38521 tactaattgc atattttcgc gaatatattt tttgaggaat ttttcaacaa aagaataaaa
    38581 gaaaatacct aaaatcgccg gcatgacagt agacgtatac tgcatcagta ctacaggaat
    38641 ccccagaaaa tcagtagctg ctttgccact gtctgaaagt aaagaggtca gattcggttc
    38701 aatcagtgct gcagcaatga cagccgcaat atatccattc actttaaacg ttttggcagc
    38761 agaaaaagca agaaagattg gcagaaaata aaagacggca ttcgacgcag cagacaaaat
    38821 atgataagtt ccgcttgttt cactaagcca gccaatcgta gtcgctaaaa caaccagacc
    38881 ttttaatata ccagaaccgg caaacagcgg aatgatcggc gtaaagattt ccgatatcaa
    38941 accaaatagt ttttcgatcg gatttagctt ttctttcgga gctgaatgat cgatttgacg
    39001 cgtcaattcc ttgtaagcag gagccaatcc ctgatccatg attatttctg catatctatc
    39061 gagtaaaatg aaatacttaa tggaagggat ttgctgtttt aatttttctg cattggctac
    39121 tggctgtttt ttcaatttga tacgaagccg agtcgcacat ggaaggacag aatcaatatt
    39181 gtcttttcct cccaactgtt caatcagcat atgaatctgc tcggacagta ccatctgttc
    39241 aggattcatt gtcgtttcca ttagaagacc actccactca ctaatataat attggcgtaa
    39301 ggtgtcattt cgcctgtacg aataaatgca tgacagttat tcaactcttt tttcatttct
    39361 tcatgtggga taaagctaat cggtgtatct ggtaacaata atttgatttt ttccaatatt
    39421 tccgggttca ttgtctttat ttcttccgcc aattcaatcg attgtacctc aagttcttct
    39481 aaaacgtttt ctaaaacaga taaaaaactt ggaagaccgt ttgagacagc caaatctatt
    39541 ttttcagtac caaaggggac aggcatgcct gcatccccga tacttaattt atcaaagtgc
    39601 cccatttgtg cgattactcg tgaaatatct gaattgatta cttttccttt tttcatgttt
    39661 gtttcttcct ctcattgtta attttagact gctgattgtt ctaaagcttc gcggtaaggg
    39721 atagaaggtt gcgcaccata agactgcaca gcaacagaag aggctagatt tccataaagg
    39781 atcgcttctt tcaagttact aaggtctggc tgaagtgtgc tgcttaatgc gccaataaat
    39841 gtatcacctg cggctgttgt gtcaacggca tccactttaa aagcagggac gataccgtgt
    39901 tctttttctg tatgatagaa agcgccggca ctgcctagtg taatgattac tgttccaata
    39961 cctaattcat ggagtttttc ggctgctgcg accaaactgt tatgatctgt cactctgatt
    40021 cctgtaatga tttctgtttc tgtttcattt ggtataatga tatctgtctt ttcaagtagt
    40081 tcagtaggaa tcgtttcacg agctggtgca ggattcagga tcgttgtttt tcctgcgtct
    40141 tttgcaatag aaaaagctag gatcgtcgca tctaagctac tttcaaactg agcaataata
    40201 aagtcactgt ttgtaatcag ttgcttgttt tttaggactt cttgttcatg gaaagcatta
    40261 tttgcccctg catggatcaa gatactattt tctcctgctg cgtctaccat aatcattgct
    40321 tgtccggttg tggcgccttg gatttcagct atcccagatg tatcgatgtt ttcttttgtc
    40381 agtagatcta ataactgttg tccttcgctg tcagccccaa cgcctccaat aaaagatgtc
    40441 cgtgcgcctg aacgtttggc tgccactgct tggttggcac ctttacctcc gccagcgtga
    40501 aaaatctcat gcgcatgcat cgtttcgcct ggcttgggca tgttggttaa acgaagggta
    40561 gtgtccatat taatacttcc aattactgta accgtgttca ttttacaaac ctccaactat
    40621 ctcatttatt ttgatgggaa aattattttt taaaacgttt tagtaaaacg ttttaatagt
    40681 tcgataaaaa tatagcacac cttgaaagct atttcaaggg tgcagttgaa aatctttttt
    40741 ctaatcgtac aggtaatctt ttttcttccc attctttttc tgggaattgg atccgatcta
    40801 acaataactt ggcagatgtt tgtcccaatt cgaagattgg ttgtgcgatc gttgtcagtt
    40861 tcggcttgat atactcgcac atatctatat tatcatagcc aataatgctg tagtcttcag
    40921 ggatgctttt tccagcttcc tctaatccgc gatataatcc aaaagctaat tcatcattta
    40981 aagcaaacac accagttgca gcagaatcaa gaagttctgc tgtgatctgg tagccacctt
    41041 gttttgagaa ctgtgtaggt aagaggatca gttggtcgtg agggatttgg taaacatcaa
    41101 gagcagattt aaacccctca atccgagcgt ggacattttc cggtggattt tcaggataga
    41161 caagggcgat cgtttgatgt cctagactaa gcaagtgcat gcccgctagg taaccaccgc
    41221 gaaaatcgtc tgttcgtacg gcatcactaa atccttcaga ttttttctga tccaaaacaa
    41281 tgaagggccg gccttgtttt ttcaaatttt cgttgattgc atctgtagag acagcagaag
    41341 tcgcgataat gaatccatcc acacctcgac gtgtgagctc ggctagatat tcgatttctt
    41401 tttgatgatc agaatcagca ttacataata tcgtgacgaa attctgctta tacaagatat
    41461 cctcaattcc tcgcatcagt gtactgaaga atggattcgt gatatcagga acgagtacac
    41521 caattgtttt cgtttccttg gtaatcattt gtctagcaaa atagtcaggc tgatagccta
    41581 attcttcttt ggcagctaaa actttctgaa cagttttcgg actgaatttc tccgtatttc
    41641 catttaatat caaggagaca gttgtgattg aaacgccgga aagtttagcg acttctttga
    41701 ttgtgatctt ttttttcacc atgttcatac tcctatctaa atcctgtggt tattttccca
    41761 tgaaatcaaa aagaagtaaa tgaaaatcac gtgtgccagt acttatttgc tttgttggat
    41821 agcttcgatt atttgtttcg ttgagcgtac tcgtgcagtt ttcggaaata tcttttttat
    41881 gaacaattga tgggattctt tgtcacgatc actgcaagca tcttcagcaa gcacgatatg
    41941 gtacccgtgt tgaaaggcat ctaatgcagt agcatataca ccgtttgttg tcgctacacc
    42001 gcttaaaatg atagtttcga ttccacgccg gcgaagttga agatcaagag aagtaccaaa
    42061 aaaagcactt ggattgtatt ttgtgatagt caacatatta tctgtatctt tcaaagaatc
    42121 cttcaaaagt aaatcagtga attctggagg tacagaagca gtcattccac ccatatcagt
    42181 ttgttgactc aaataaggga aagtggagat gtctacgttc actaaaactt tcaaggcagc
    42241 ggtacgtttc aatgttctta ctaacaaatc attgtttgtc agaatagatg ctgcagtgtg
    42301 tggagagagt gtaccgttat ttacgatctc tttttgcatg tcgatcgcga caaaagcagt
    42361 ttttgataaa tcaaaaatca tattttcctt taaatcattc atgatgttcc ttctttcaaa
    42421 taacaatgtt ttttatgtaa gtaaatcata aatcgtttat ttgtcaacta aattaaagga
    42481 ttcctttctt tagtctagag aaattcagtg gtaaaataaa taagtgtgag aaaacgcatt
    42541 cgatgcatag atatagcgag aaatatcgtg aaaatggagg aatcagagtg aagtatgtcg
    42601 aagtaaaaaa tgaatacaaa cgatacaaaa tgggagaaac gaccattaca gcaaatgacg
    42661 atatttcttt ttcaattgaa aaaggcgaat tagtgattat acttggtcca agtggagcag
    42721 gaaagtcaac gattcttaat attttaggtg ggatggatac acctgatgaa ggtcagatca
    42781 ttattgatga cacagatatt gcgcaatttt cagataaaca gcttactgct taccgacgaa
    42841 cagatgtagg gttcgttttc caattctaca atctcgttcc taatctaaca gcaaaagaaa
    42901 atgtggaatt ggcaacggaa gtttctcctg atgcgcttga tcctgtagag gtattgaggc
    42961 aagtgggcct agctcatcga ttgaataact ttccgtccca attatcagga ggcgaacagc
    43021 agagggtttc aattgctcgt gcactagcta aaaatcccaa acttctttta tgtgatgaac
    43081 cgacaggcgc gctggatttt gaaacgggca agcaagtatt gaaattattg caaaacgcta
    43141 gcagacaaca cggcaatacc gttttgatca ttacacataa ttctgcttta gcgcccatcg
    43201 ctgatagagt cattcacatt aatgatgcta aagtaaggtc tgttgaatta aatgatcatc
    43261 cttcatccat tgatgagatt gtatggtagg gggattttat gaaaacaaga acatatctta
    43321 aagcaggttt tcgcgaaatt cggcaatcta aaggacgatt cattgcaatc atccttattg
    43381 tcatgctagg aacactttta tttgtaggtg tcaaaactgc tggacctgtc atgcaaaaaa
    43441 cgatggatca ctatgttggt acagcaggac tttctgattt gcaaatcgtt tctactggtg
    43501 gcttgacaca aaaagatatt gctcaagcag aaaagatccc agatgcaaaa gtagaaacag
    43561 gaaaacagat ctattattcc aatactggca aaaacgaagt gattcaaata ttttcttata
    43621 acaaaaataa caagcagaac aagctacaat taactgatgg aaaactcccg gaaaaaccaa
    43681 accagcttgt attggatgaa aaagcggaag atgaagggta taaaatcggt gatatttatc
    43741 agattgattc agatgaattg aaagaaaaag agtataccat cacgggcttt gtccgttctc
    43801 cattgttcat caacaatttg gagagaggat acgcgaacgt cggaaatggt agcgtcgatt
    43861 attttgtcta tttacctgaa tccgttttta aaacagatat ttattctgtc ttatatcttg
    43921 attttacaaa cgtaaagaat ttgaatacgt acagcaaagc ttacaaagat aaaatggagc
    43981 aaaatcaaga aaaggcagaa aagtatttac aagatcgacc cgaagaacgc ttagaagagc
    44041 tgaaaaaatc ggccaacgaa gaattagaac cggcacagca aaaaatagcg gatggcaaag
    44101 cacaaacaac gcaagcaaga acacggttag aagatgctaa aaaacagtta gcgcaacagt
    44161 cagcaactat ccagcagctt cctgaagcgc aacgaacaat cgctgttcaa acattatctc
    44221 aacaagaagc tcaacttgct gaacaagaaa ggcaattagt tgagaaagaa cgtgaactgg
    44281 cctctgcaga ggaagaattg aatcgaaatg cagaaaaaat tgaaaattta actaaaccat
    44341 catacctata caatgaacgt tcagaaaatg tcggttttca agaatttggt gatctgtctg
    44401 atcgtatcgc tgcgattgcc aatgtttttc ctgttttctt tttctttatt gccgcattga
    44461 tcacctttac gacaatgaca cgtatggtag aagaaaaccg cagggaaatc gggacattaa
    44521 aggcgttagg ttatacgaaa gttgaaatcg caaaaaaata tgcaatctat gcaagtctag
    44581 cctcagggat aggtattgtt ttaggaacga tattaggaac gaatcttttg ccaaggatca
    44641 tttttgagct gtctaacgag cggtatgata ttgggagtgc gcttattttt tatgactggc
    44701 cgccgatttt acaagcagct gccgcattct tcattgccgc atttggcgct gcaatgatcg
    44761 ttttattcaa agatttaaga gaacggcctg ctgcattgct tcaaccgaaa gcaccgaagc
    44821 cagggaaacg catttttctt gaatacatca ctccgctgtg gtcacgcctt agctttaatc
    44881 aaaaaatcag ttaccgaaat ctcttccgat ataagtcaag aatgcttatg gcgattatcg
    44941 gaattgctgg atgtgccggg ctgatggttg caggcgtggg attgaaagat tctctaagtt
    45001 ctgtttcagc caaacaattt ggaccgatca ttgactatca agcaatcgtt acattggatt
    45061 catccacgga agatagtcgt caaaaagcag cggatatgct gcagaagcaa gaaaaagtaa
    45121 atgagatcct agctattcat actcaaaccg tagaattgag aaaaaaagga catgcttcgc
    45181 aaagtctgac agtgatcgta ccaagtaaaa cagctgattt ggatcgtttt atccatttaa
    45241 ctgatcttga agagaaccca cgctccttgc ctaaaacagg aatcgctatt acgcaaaaag
    45301 tagcagaact atttgactta tctacaggag atcatatttc gctttatgat gaagatcaaa
    45361 aagaatggaa agtcgaagta aaagaaatcg tgaaaaacta tctaggtagc tttatttata
    45421 tgtcccctga atattatgct tcagttactg atcaatcgat gatggataat gcatttttgc
    45481 tgcgtacaga taagatgaca acaaaagaag aagaaatgct ttccgagaaa ctgcttgcca
    45541 cagacgctgt gacaaatacg tctttcatgt ccaaacaaat cgaaacgcaa gaagaatcaa
    45601 tggcgaactt agactcggtt gtcttaatct ttgtcgtatt atcaggactg ctggctttta
    45661 tcgtcttgta taatctaact aatatcaata tttccgagcg tgtacgcgaa ttatctacaa
    45721 taaaagtcct tggatttttt gataaagaag tcacgatgta cattgttcga gaaaatatca
    45781 tctttactct tttaggaatc atcggaggat ttggtgtagg atatatattg actgacttca
    45841 tcttgcaaca agcgtctatg gagaatgtca tctttccatt agtgatcaca tggtgggctt
    45901 atgtcttatc agcaggatta actatcgtat ttacggttat tgtgatgatt gttacccatt
    45961 tcaaattaaa gcatatcgat atgattgatg cgttgaaatc gaatgaataa aaacagaggt
    46021 tgtgaaatca gcggtttgac ctgagtatta gagaaacaaa cggcaaaata gtttttcata
    46081 ttttgccgtt ttttgagcta ataccgaagg acagctgatt gaacaccatg aatcagaggt
    46141 tgtaaaaaag ccgtcctgca tcgagcaaaa agaatactca aataaaatta gctcctcatg
    46201 ttttacattt gagaagactg attgccgtag ataaaagctt ttgaacacca tttatagaag
    46261 aaacaagaat attttgctat tttaaacaag aggatagaca aatacatgtc tgatctcttg
    46321 ttttttattg ggcaacagac ctcttagaat gtataaagat aaattttaca aacgctttcg
    46381 gttcagttat aataataaat gataacttgt atatataagt tgtggtaggt aataaatcag
    46441 ttgaatggag gtaaatatcc atgacattta aagacaaagt catttaccaa atctatccca
    46501 aatcttacaa agacagtaat ggtgatggaa ttggagattt aaaaggaatc aaagaaaaac
    46561 tcccgtatct gaatgatcta ggcatcgaca tgatttggct aaaccctatt tatccaagcc
    46621 ctcaaaaaga caacggttat gatatctctg attacacagc catcgatcct atctttggta
    46681 cgatggagga attcgaacag ctaatagccg aagcaaagaa ataccggata gaaatcatgc
    46741 tcgatatggt gctgaatcac gtttcgacag aacatgaatg gttcaaaaaa gcactagcag
    46801 gggaccagca ttatcaggac ttcttcattt taagagaaga gccaacggat tgggtatcaa
    46861 aatttggtgg aagtgcttgg tcgccatttg gagatacggg gaaatattat ttgcacttat
    46921 atgatcggac acaagctgac ttgaactggc ggaaccaagc agtaagagag gaactattca
    46981 aagttgttcg tttttggatg gaaaaagggg taaaaggatt ccgtttcgat gttatcaatg
    47041 ttatcggtaa agacgaacaa ttgaaaaata ataaagaaaa tgatggcaaa cctgaatata
    47101 cggacaaacc aatcacccat acgtatctaa aagaattgaa ccaagcaacg tttgggacgg
    47161 attccactat catcacagtc ggtgaaatga gctctacgac gatcgaaaat tgtatcttgt
    47221 atacagaacc tagtagagaa gaattatcaa tggtctttaa tttccatcat ttgaaagtcg
    47281 attatgaaaa tgggaataaa tggtctactc ctccatttga ttttgcagaa ttaaagcgac
    47341 tatttcatac atggggagaa aagatgagtg agggtaacgg ctggaatgct ctatttttta
    47401 ataatcatga tcagccacgt gcgctcaatc gatttgtagc tatcgatcag tatcgaaaac
    47461 aaggagcaga gatgctagca accatgatcc atttgaaccg tgggacgccc tatatttata
    47521 tgggagaaga aatcggcatg atcgacccag actattcatc gatttccgac tatattgacg
    47581 tagaaagttt gaatgcttat aagttactcc ttgaacaagg attcacagaa aaagaagcgt
    47641 ttcgtcggat caaagcaaaa tctcgcgata actctcgaac acccatgcaa tggtcagaca
    47701 cagaacaagc aggatttact acagggaaac cttggttaaa agttggaggc aagctagaag
    47761 agattaatgt agaaaaagaa ctatcttctg aagattctat tttgtcctat tacaagaaat
    47821 tgattcggct gagaaaaaca tatccaatcg ttgcacaagg agattatcat gcttatgaag
    47881 caaatcatcc ggaagtttat ggctttcttc gccaatttga aggacagcag ctgcttgtat
    47941 taacaaattt ctatgataaa gatacaagga tcgccatccc agaaggattc acagaagcca
    48001 atattcttat cgataattat tccgatactg ttcttcatca aagtctggag ctaaaaccat
    48061 accaggcaat tgctttgctg aaaaattaaa acaaaaacac aaaacgtcgt tcctacgagc
    48121 tattctcgta agatcgacgt tttgtgtttt atttaaaata atcttcttca gaaaatgaag
    48181 agttttcata ctctccttta tcgccataaa atttattgta gcgttgtttg aatttacgga
    48241 aaataaaagt aacgagtaat ttgactaacg tatagatagg aataccaaaa ataacgccca
    48301 caattcccat tagatcaccc atgacaagta aaacgattaa gatcgtaatc ggatggatct
    48361 gtaaacgatc acccattact cgaggaatca ccaagtcacc gtggagaagt tgtacggcaa
    48421 accagacaat gacaaatttg acagccataa aagtagagtc ttggaatgca atgatcaaac
    48481 ctggaaagaa ggcgataaac ggcccaatat atggaatgat acataacaac ccagcagaaa
    48541 tagctaaaac actagcaaat tctaatccga tcagtaagaa acaaacccag tagactgctc
    48601 caaggataaa tgaagcaatg atttgcccct ttagaaatga acccaattgt tgattagcaa
    48661 tctttgtcaa atttttaaaa tctgtacgaa atgctggtgg gacaatcgct aaaactgact
    48721 gataaaattt ttgaggatct ctcaaaagga aaaaagcgac aattggacca gtaaataaag
    48781 taataaaaat agctgtaaca gttgaaaaaa tgttgctgat ccgttgtgcg ccactttccc
    48841 aataatttcc aataaatcca gctatatcag acgtcacgtc gtcaatagaa gcgaaaaatt
    48901 gattaaatga atcggcaaaa ggtgttttct ccaaaaattg tcggagactc tcttgaaaat
    48961 cttgaaacaa atctggcaaa ctactgatca atgtctttgc ctgttcttca agaaaaggaa
    49021 taagccatat gcttccaagc cccaataaaa ataaaataat cacataaacg ataatgactg
    49081 acaagttcct cgaaaggtgc ttgctcaaac gtttgatgac tggttccaaa agataataca
    49141 agatcagtgc aaacaataat gggggcaaga tagtagcgat aatgattccg acaggcttga
    49201 atataaacga gatctgatgg aaaaaataaa tgatcagcgc aagtaagata actattccta
    49261 aaacatagta agaggttttt cctcctaaaa agcggatcaa acgattcgtt gtatctgttt
    49321 cttttttcac tctttttcct cctttatcta taaattcatt atagacaaaa gcgggtataa
    49381 aaggaaaatg atagaggttg attgcaaaaa tatcgtcaat cattcatata aactttctca
    49441 tcaattaaaa taagaaaacg cttgacagtt cttttaacca tatgataaat taaatcctgt
    49501 tgctaaaacg taactattac ggtttgcgaa aagcgaatcg gaacattcaa atgaaagaag
    49561 atgagaagag gagtgtcata aatgaaacga ttgataggta tattggccat gctggtttta
    49621 gcaggtatgt taactgcttg cggggcaagt gggaaagccg aggacagtaa agaaaaattg
    49681 agcgtgatga cgacctttta tcccatgtat gattttacaa aagcaatcgt cggggatgaa
    49741 ggagaagtag agttattgat tccagcaggg actgattccc acgattatga accttctgct
    49801 aaagacatgg caaagatcca agatacagat atctttgttt ataatgatga aaacatggaa
    49861 acatgggtcc ccgcaatcca aaaaacatta caagaaggca atgtccatac gataaaagcg
    49921 acagaaggta tgcttttact tccgggaagt gaagaaggac atgatcatga ccatgaacac
    49981 ggggaagaag gccatacaca tgagctagac ccacatgtat ggctagctcc aagtttagca
    50041 atcaagcagg tagcaaacat acgtgatcag ctgattgaag cttacccaga aaaacaggaa
    50101 gtatggacaa aaaatgcagc agcctatacc gagaaacttc aagcactcca tcagctttat
    50161 caagaaacct ttaagcaagc aaaacaaaga tcttttgtca cgcaacatgc tgcattcaac
    50221 tatctagcgt tagaatatgg attgaatcaa gtatcgattg ctggtctttc ttctagtgaa
    50281 gaaccgtctg cggcgagaat tgctgaactg aaacactttg ttaaagaaca tggaatcaac
    50341 tatatttatt ttgaagaaaa cgccaaagat agtattgcca gaacgttagc aaacgaagca
    50401 ggtgtatctt tagaagtatt gaatcctttg gaaggattga cgaatgaaca aatagaaaat
    50461 ggagaaaact atctttctat catggaagca aacctcgaag cgttgaaaaa aacgacagag
    50521 acagaaaacc cgctagaaga tactttgacc cccaaaaaag aaaagagcgt ctacaacggt
    50581 tattttgaag acgaggatgt acaagatcgc tctctttcag attgggcagg aacttggcaa
    50641 acagtagata cgtatgtaga agatgggact tttgatcctg tttttgaata taaagaaaaa
    50701 ttgaatcagg ataagacagc acaagaatat aaagaatatt atacgaaggg ctatcaaaca
    50761 gatattgctt caatcaagat cagtgagaaa gagatggcat ttacgtttaa gaatggaaca
    50821 acgaaacgtt caaattatcg ttatgtaggg aaagaaatct tgacctacga ggcaggaaac
    50881 cgtggcgtaa ggtatctatt cgaagcagaa gataaggaaa gtggcgcttt tagatatatt
    50941 caattcagtg atcacacgat tgcaccggct gactccgaac attttcattt atatgcagga
    51001 aatgaaagtc aagaagtatt gctgaaagaa atggagaatt ggccaacatt ttatccagat
    51061 acaatggatg gacgtgaaat tgcacaggaa atgcttttgc actgataggc aaaaaagaac
    51121 cgaagggttt ggatcggctg gtcatttagt cggtccaaac tattcggttc tttttatgaa
    51181 atcaatttct ttaatccacg gatagaggct gggatggtct cgccgtttgc caacgtgact
    51241 tctctggttt ttttattaat cgaccgcaca ttttgtgtat tgataacata ggatttatga
    51301 caacggataa aattccgatg cattttctcg atttctttga tcttctcata gaattgtaat
    51361 tgggcatgag tcaaatggac ttccagttta tgtggcgctc ctagcgaagt agaaaaatat
    51421 tggatgtctt tgatcggtaa aaatttgatc tcatcattaa actctatttt gaagatatct
    51481 ttttggattt gggacatttg aaaatcttcg aaaattttgc tcaggatatg ctcgacattt
    51541 tcttttaaat cataaagatc ttctttgacg atataatcag tcggttcgat gttgcttttt
    51601 aagattttca tcgacatttc cgtatgggaa gtcacataga ttatttttcc taaaggatct
    51661 aattcacgga tcaatttacc gatcgctaca ccatccattt cactgtcttc tagttcgata
    51721 tctaaaaagt aaataccaaa agaagaagtt ctgatcttgg ctgcattgag aagttcttcc
    51781 ggatcagagg tagaaatatg gacataactg tctaagtttt caatcatgat tctatttttg
    51841 atcaccgttg ataaaacttc taattgtttt ggatcgtctt cacaaatata aatgggaatc
    51901 atatcattac tcctttattt tatttgtata taaatcgttt gagaaaaacg ctggttttca
    51961 attgatgttt caaggaaaag ataaggattg gtctttgtca attcatccaa gataaacaag
    52021 ccaaggcctt cgttttttgg atttgttttt gttgagaacc ctttttgttt caactggttt
    52081 aacggtaatg tatttgttgt tgtattgatc acacgaatca tcacggaatg attcttcttg
    52141 agtatggtca attcgatttt tttcttcttt gcagcactag ctccttcaat cgcattgtcc
    52201 aagataatac caagcatacg tacaaggttt accgtatgtt tcgtatccag ttcgattggc
    52261 tcaggaaaat gaagctggac gatcaaccct ttttcctgtg ccatcatgag ttttaatgac
    52321 agtaaactac ggatctcagg aactttcata ttatccaaca tacttagatt ggccggaaaa
    52381 aggttcatca tctggcgtgt aggtaatatc gtttgatcat aataagaccg taatccttct
    52441 aagtcgcctt ctgaaatata gctgtgaaga gaaaaaagca gattttggta atcatgtttg
    52501 aattctctca agtcttgata gtttttttct agatctttga tataatcctg ttgcatcctg
    52561 acttgttgct ctaataactg atcagctaat ttcttattag agattttcca ataataaacc
    52621 aagatgatca ggccaacaca cagatataga atgaaaaaac tcgtattcaa agctaagaca
    52681 tcaggtgttt caccagaaaa acgggtataa aaaataatga tatagtatgt aaataatatc
    52741 aatgtcccga tcgtaccgat catacggcgg tccacagtgt gaaaccaagt ctctaacgat
    52801 ttttttaaac aaaaagctaa taaagcagat aaaaaagcgg ataagacaat gtgaaaatac
    52861 tgcaatgaat ctcctggttc aataaccgaa gatttcaaaa caaaaaaatc gccgatgctt
    52921 gttaagtgat ctcctaaaag cgacgtcaat aacccaaaag gaagcgcgta aaccaattgt
    52981 tgacgcgaag tcgaaaagaa gaaaaaagta agcatcagca ttcctatagc aattagagca
    53041 gtaaaaatcc ctaaaatcga atagacccat ccaatcaaaa tacaagaaat cagaatgact
    53101 cccgcaaaca gatatttgta tcggatgtga tggtcaatca ataagataca aattgtgtaa
    53161 atcaaatatt gcaacgctat ggaccataaa aacataaagt acagctccca ttgattttta
    53221 ccaaattata gcacaattgg cgtattattt ttttaatttt tcagggattt tgaattcaga
    53281 actcatacca aagcagcagc cagatacaaa aaagtcagat aagttagtta attgtttaat
    53341 gattgttttt ttcatgaaag attcctccaa aattcatcaa gtagtttgta ataggaagca
    53401 acgtagctat ttctaaaaca atcccaagta gcaaataggt agaaaagtaa gaagctgaga
    53461 aaaataacgt aatgagagaa aaaattagaa acatgctgat cgccttcttt aataatgggg
    53521 gacgtttctc gggttcaatg ggattgattg ctgtacctct tgatccatag cgaagcagta
    53581 acaaaaagca gctgattaac aaaaagacca tcattgggaa ggagatagtc aattgcagat
    53641 gtttcatcac caagggaacg ccccataaat aaagtagtgt ccacagcaga caaacgaatt
    53701 cagagtcgga atgcaatcca taagcatatc tgcggatgca gatataagaa agaaaggtga
    53761 ttcctgtttc cgcccatgag cctgtcacca gagaaaagac tagaacgacc gctagttttc
    53821 caagattatt caataaaact tctaagacaa attttacttg gacatacacc atatcttctt
    53881 ccgatttgtc tccgctgaat aaggcttcta ataatttgtt cacaacttta tctttcatct
    53941 catacgtacc tccaccacaa attttatgct aaaaaaaata aaaaacgtct tttgtttcat
    54001 aaaaagtggt tttgacatca aaaccgtcat gatctcctaa aaaaatcttc gttttccaag
    54061 aaccttacca aaaaaagtga cagttttgat acgaaaagta ctttttatga caaatttgtg
    54121 ctttttcgag tgatttatgt taaaaaatta ctgaccttat aaaaggtgat ggagggattc
    54181 ttatggaaaa tgaacaattg tttgaagtcg gcgaaacata tgcatttaaa tctgatttat
    54241 ttgaatcacc aataaaagga aaaatagaaa aaatctatga aaatagtgtg atgatacgtg
    54301 tcatgaaatg cgcagtagaa gacaatgata ccgctcataa tctgcactgg cagttagttg
    54361 tacgcaaatc aagtacaata gaagaataaa aacagataaa atgttgagag cataaagagg
    54421 ttgtgagtct cccgtttagc tctgagttac aaggaataat gttaaaaaac agcttttcat
    54481 gttttatgaa tattgccttg tgaccgaggt gttggcagga gaatactgtt tatttgaaaa
    54541 aagggctgcg acaagacttt tgtcacagcc tttattttca tgaataggag ttacttttgg
    54601 ctacttttag agaagtagct tttaaggagc agataaagtc aaggctttta tttctataaa
    54661 tgaagtagta aaatgaagaa cagattttat tacaggaagg atgccgataa ttgtgatcat
    54721 tgttgcagga acgatcggtg cagggaaaag cacactaact gaaatgcttg cccaagattt
    54781 agaaacaaaa cctttttatg agaacgtgga agataatgaa gtattgccgc tattttattc
    54841 taatccagaa aagtatactt ttttattaca gattttcttt ttgaacaaac gctttttggc
    54901 aatcaaagat gctttcagcc atgatgacaa cgtactagac cgctccatct acgaagatag
    54961 tatgctgttt catttgaatg cagatttagg gcgtgtttct gaagtagaag taaagcagta
    55021 tgaaggtctt ttagaaacga tgctgaagga gttagaagaa atttcgccac aaaagaaacc
    55081 agatctactg gtttatattc gtgtatcctt tgaaactatg ttagcgagaa tcaaaaaaag
    55141 aggccgggaa tatgaacagc tagaacaaga tcctgaactc tattcctact ataaagaact
    55201 caatcgacga tacgaagagt ggtatgaaca gtttgatata tgtcccaaaa tagtgatcga
    55261 tggggacaaa tatgatttcg tagccgatcc ggcatgtgga gaacaaatcg tacaagaaat
    55321 caagatgcgg gcaaagaaaa tgacagaaga ggcagatgcc cagtcgatcc gctacactac
    55381 aaataacccg aaagcaaagg gaatctttac aactaaataa tataaaaact aagcaaattg
    55441 ctcactcgca gatatttctc tgtgagcagc atcttgctta gtttttttga ttcattgaga
    55501 tcaaatagtg aaccgattcg tatgataaaa ttgccataaa tgaaatgtct gagtgagggc
    55561 catgatcaaa aataaaatgg aaacgatcaa aatcgaatgg gcgccaaaca gatgatgaca
    55621 gaaaacaagg acaatagctc ctgcgataaa ggcaagaacg acacttagat agtggaaaaa
    55681 tcgatcaaaa tctgcttttt ctttagaaat acaataatca tataagttcg ttgctacccc
    55741 ttttaaattt ccagttgtaa ataaattagt gtaggcacgc ccgttgattt tatcaaaagc
    55801 tacccactgg atcgatgcag taaaagcaat caaaatcgtt actaatgcgg gatgctcatg
    55861 gacgggtgtc aaactaacaa agaaaaaaat cactgcttca taatatagaa caaacaagcg
    55921 ccagaaatgt ttttcttttt ttctaaaata atcaattaga tactttgtta gaagaatccc
    55981 taagaaaaag aaaagggtag acaatacttt tttaccaaca cttagccatt ctccgtcaaa
    56041 tgcttggatg atcgatagaa tgatattgcc agtttgagca gaggcaaaag caccatattg
    56101 gatataagta taactgtcca tggctcctcc gataaatgtc agcaaaagtc ctattgtacg
    56161 cccctcgtga aaggaagtct gcattttttt catcaccttt tatgaatatt tttgtcttcc
    56221 ttatcttagc ataaacgatt gaaaaaaagg aaaaattttc agaaaactaa aatcatatcg
    56281 cttcccattt tcatttttag aaaggtctgt tagtataaag aaaaaataga caacgaaact
    56341 ttaaataacc aacttgaaat aaacaaattc aaagaagaaa gatgggaagg aaatcaatca
    56401 gttgaatgaa aaagaaattc tccgattaca aaaagaattg aaacgctggg agaacaagta
    56461 taaaaagctt caacaagtct ttcggctaca agaagaaatc attgctacct atgaagcggc
    56521 ttatggcgaa gcacccggac ttattgagga agaagtacaa gtaaaagaaa gtgaaggaaa
    56581 cagctctttc tttaaaatga tggagaaacg ttcctataat cgcttcaatc aagaacaaaa
    56641 agcagctatc acatatgata tgaagaaaca tcttcgaatc atagcaggag ctgggagcgg
    56701 caaaacgcaa acaatctgtg caaaagctgt ttatctgatg acgcaaaaac aagtagacga
    56761 agaacgcata ctaatgatca cttttacaag gaacgctgca aatgaactaa aaaaacgagt
    56821 agataatttc agccagagaa aaaccgctgt ccatatcgga acattccata gtatcttttt
    56881 taggatatac aatgaaattt gtcgaaaatt tcctgaagta gcaatgcaag gaatacaagg
    56941 agacttttca aaggatactg cacaaaaagt gaatgctgtt ttgcaacagc tgatacgtaa
    57001 atataatctt tatatatttg atcaatatgg agaaaaaacg attgcttctc ggttagacta
    57061 ttggcagaat atgaaccttt cagtagaaga aatggttcag ttagttaaag aaaaatatga
    57121 ttccattgat aagaatgcac gacagccgat atcagaacga ctgtatggat tgctcacgga
    57181 actccaagaa caaaaaagaa gccagcaatt gctagagttc aatgatgtac tccaaaatct
    57241 gaaaagcgct ttggagaatg aggagatcca gcattatatc ggccaaaaat atgattatct
    57301 attcattgat gaatttcaag atactaatcc acttcaatgg gaaatcgtga aacggatgac
    57361 aaaggaaaac aggatcaagt tgattgttgt tggagatgat gatcagagta tctatggatt
    57421 cagaggatca gagccagcat acatcaagaa ctttgaaaaa gaatatccta ccaaaactct
    57481 tttcttatta actaattatc gttctcgggc agctattgtc caaggagcaa atagattgat
    57541 cagctataat aaaaatgatc ggataccaaa ggcgatgatt ccagcacaaa aagaagcagg
    57601 tgttatggaa gcctatttgt tttctgatac taaagcagaa agtcagtggc taatcaaaca
    57661 aattcagaca tttattcaaa aaaatgggaa atataaggaa tcaatcatat tataccgatc
    57721 agcttcacaa acgcagcagc ttgtccaatc tctgctgaaa acgaatattc cttttgtact
    57781 ggaagcagat agtccatatg aaggtatctt tggtatcaag tctttccaac atttctatca
    57841 aaaaattgtt cattggcaaa cagcactgaa tctgcaaaag aaaaatcagg cctaccagca
    57901 attgctccgg cagctgatgg cagactgcta cctgaaaaaa agtgaatgcg atcaatactt
    57961 ttctcctaaa tatcaaaata tatccgttgc agattatatt ttgtcgataa gccccaattt
    58021 aaaatcgaaa tctgcagaaa tccgagagtt tgaggaagcg ttgcgtaaaa cagaccagca
    58081 tcaagaaatc tattcgcttg ttcaaagcta tcttcatctg ccacgtatag ctaaggaaat
    58141 cgaccagagt gatcaagaat ggatacgaga agaagtaaag cagcatgcta ctttcagaag
    58201 actccaagcc ttatcgcaag aaacccaagc gacagctaaa gaattaaaaa cccgcttgat
    58261 tgcttacaga caaggaaaac ttgatgcact atgtatccag agcattcaca aaagtaaagg
    58321 tcttagttac cgaaatgtct ttttgattgg ttgtaacgaa ggaagtttgc cttataacgg
    58381 agcgacagaa aagaaagtaa tcgactttaa tgaaataaaa gcagaaccag taacgacgat
    58441 agaagaagaa cgaagactat tttatgttgc gatgactaga gcaagaaatc gtctgtatct
    58501 atgtgtacct caaatgaaaa attcaagaaa attgaaagta tcgagattta tcaaagaaac
    58561 cgggagtcgt gtgaaaaaag gaaagtgata gaagagaggg tgtgacagaa gtggtcagtt
    58621 tcaagaaaga agaaggaatg tacgaaaatt gcttctcaaa tttttgtgaa atttcgactt
    58681 atttttcgaa gaagctactt
//
LOCUS       DAZUMK010000003        58308 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000003 DAZUMK010000000
VERSION     DAZUMK010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 58308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.003"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(59..187)
                     /locus_tag="ACG808_000211"
     CDS             complement(59..187)
                     /locus_tag="ACG808_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFS5045874.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="ACG808_000212"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="ACG808_000212"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFS5045875.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="ACG808_000213"
     CDS             complement(1674..2729)
                     /locus_tag="ACG808_000213"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFS5045876.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="ACG808_000214"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="ACG808_000214"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFS5045877.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="ACG808_000215"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="ACG808_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFS5045878.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="ACG808_000216"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="ACG808_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFS5045879.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="ACG808_000217"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="ACG808_000217"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFS5045880.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="ACG808_000218"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="ACG808_000218"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFS5045881.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="ACG808_000219"
     CDS             10491..12050
                     /locus_tag="ACG808_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFS5045882.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="ACG808_000220"
     CDS             complement(12161..14092)
                     /locus_tag="ACG808_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFS5045883.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="ACG808_000221"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="ACG808_000221"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFS5045884.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="ACG808_000222"
     CDS             complement(15021..15773)
                     /locus_tag="ACG808_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS5045885.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15932..16141)
                     /locus_tag="ACG808_000223"
     CDS             complement(15932..16141)
                     /locus_tag="ACG808_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045886.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16152..17480)
                     /locus_tag="ACG808_000224"
     CDS             complement(16152..17480)
                     /locus_tag="ACG808_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045887.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            17616..17771
                     /locus_tag="ACG808_000225"
     CDS             17616..17771
                     /locus_tag="ACG808_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFS5045888.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(17845..18432)
                     /gene="yihA"
                     /locus_tag="ACG808_000226"
     CDS             complement(17845..18432)
                     /gene="yihA"
                     /locus_tag="ACG808_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFS5045889.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="ACG808_000227"
     CDS             complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="ACG808_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFS5045890.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20012..21301)
                     /gene="tig"
                     /locus_tag="ACG808_000228"
     CDS             complement(20012..21301)
                     /gene="tig"
                     /locus_tag="ACG808_000228"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFS5045891.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21455..22387)
                     /locus_tag="ACG808_000229"
     CDS             complement(21455..22387)
                     /locus_tag="ACG808_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045892.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22544..23203)
                     /locus_tag="ACG808_000230"
     CDS             complement(22544..23203)
                     /locus_tag="ACG808_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFS5045893.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="ACG808_000231"
     CDS             complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="ACG808_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFS5045894.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGASGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24244..24534)
                     /locus_tag="ACG808_000232"
     CDS             complement(24244..24534)
                     /locus_tag="ACG808_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045895.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            24944..26749
                     /gene="glmS"
                     /locus_tag="ACG808_000233"
     CDS             24944..26749
                     /gene="glmS"
                     /locus_tag="ACG808_000233"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFS5045896.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(26821..28176)
                     /gene="glmM"
                     /locus_tag="ACG808_000234"
     CDS             complement(26821..28176)
                     /gene="glmM"
                     /locus_tag="ACG808_000234"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFS5045897.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(28200..29369)
                     /locus_tag="ACG808_000235"
     CDS             complement(28200..29369)
                     /locus_tag="ACG808_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HFS5045898.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYEFDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(29366..30253)
                     /gene="cdaA"
                     /locus_tag="ACG808_000236"
     CDS             complement(29366..30253)
                     /gene="cdaA"
                     /locus_tag="ACG808_000236"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFS5045899.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(30442..34122)
                     /gene="nifJ"
                     /locus_tag="ACG808_000237"
                     /pseudo
     CDS             complement(30442..34122)
                     /gene="nifJ"
                     /locus_tag="ACG808_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
     gene            34320..34841
                     /locus_tag="ACG808_000238"
     CDS             34320..34841
                     /locus_tag="ACG808_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFS5045900.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(35540..36105)
                     /locus_tag="ACG808_000239"
                     /pseudo
     CDS             complement(35540..36105)
                     /locus_tag="ACG808_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(36284..36940)
                     /locus_tag="ACG808_000240"
     CDS             complement(36284..36940)
                     /locus_tag="ACG808_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045901.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            complement(37508..38443)
                     /gene="whiA"
                     /locus_tag="ACG808_000241"
     CDS             complement(37508..38443)
                     /gene="whiA"
                     /locus_tag="ACG808_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFS5045902.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(38477..39475)
                     /gene="yvcK"
                     /locus_tag="ACG808_000242"
     CDS             complement(38477..39475)
                     /gene="yvcK"
                     /locus_tag="ACG808_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFS5045903.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(39472..40356)
                     /gene="rapZ"
                     /locus_tag="ACG808_000243"
     CDS             complement(39472..40356)
                     /gene="rapZ"
                     /locus_tag="ACG808_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFS5045904.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(40491..41222)
                     /locus_tag="ACG808_000244"
     CDS             complement(40491..41222)
                     /locus_tag="ACG808_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFS5045905.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(41226..42491)
                     /locus_tag="ACG808_000245"
     CDS             complement(41226..42491)
                     /locus_tag="ACG808_000245"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFS5045906.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(42754..45570)
                     /gene="uvrA"
                     /locus_tag="ACG808_000246"
     CDS             complement(42754..45570)
                     /gene="uvrA"
                     /locus_tag="ACG808_000246"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFS5045907.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(45580..47574)
                     /gene="uvrB"
                     /locus_tag="ACG808_000247"
     CDS             complement(45580..47574)
                     /gene="uvrB"
                     /locus_tag="ACG808_000247"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFS5045908.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            47833..49335
                     /locus_tag="ACG808_000248"
     CDS             47833..49335
                     /locus_tag="ACG808_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFS5045909.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            49337..50074
                     /locus_tag="ACG808_000249"
     CDS             49337..50074
                     /locus_tag="ACG808_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS5045910.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(50442..51608)
                     /gene="dnaJ"
                     /locus_tag="ACG808_000250"
     CDS             complement(50442..51608)
                     /gene="dnaJ"
                     /locus_tag="ACG808_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFS5045911.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(51759..53588)
                     /gene="dnaK"
                     /locus_tag="ACG808_000251"
     CDS             complement(51759..53588)
                     /gene="dnaK"
                     /locus_tag="ACG808_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFS5045912.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(53639..54202)
                     /gene="grpE"
                     /locus_tag="ACG808_000252"
     CDS             complement(53639..54202)
                     /gene="grpE"
                     /locus_tag="ACG808_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFS5045913.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(54296..55339)
                     /gene="hrcA"
                     /locus_tag="ACG808_000253"
     CDS             complement(54296..55339)
                     /gene="hrcA"
                     /locus_tag="ACG808_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFS5045914.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(55574..56740)
                     /gene="hemW"
                     /locus_tag="ACG808_000254"
     CDS             complement(55574..56740)
                     /gene="hemW"
                     /locus_tag="ACG808_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFS5045915.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGNRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            complement(56756..56983)
                     /locus_tag="ACG808_000255"
     CDS             complement(56756..56983)
                     /locus_tag="ACG808_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFS5045916.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            complement(57303..57626)
                     /gene="relB"
                     /locus_tag="ACG808_000256"
     CDS             complement(57303..57626)
                     /gene="relB"
                     /locus_tag="ACG808_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFS5045917.1"
                     /translation="MRFFKRGNYRAMITVRVSDAKKEWLSYMAEFYGITLSELLKNYS
                     MEQLEDEYNRQTAEIAHKHWIRSGKTTVFMEESLMNLVGWNDLPFKIHARNSEAALIG
                     KYVYW"
     gene            complement(57969..>58308)
                     /locus_tag="ACG808_000257"
     CDS             complement(57969..>58308)
                     /locus_tag="ACG808_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFS5045918.1"
                     /translation="KMAAKYVNEEEFCFSKTKIYKRNPFVSEYVKRLAKGICQLCQEK
                     GPFIKDGVPYLHCHHIEYLSQGGKDVIENCIALCPNCHARIHELELPDDKEKLLKIVE
                     KRENSFSKIP"
BASE COUNT        16045 a        12669 c        10197 g        19397 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcccgc ttttttgaag taagcgtcat aataaccagc acttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tctgagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 agttggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcttcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgcttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttctgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgacagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcaattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg tgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ttccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggga attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat atcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt tgccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccgatcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatatgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atggaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaaggtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtggtg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagagac tgggaaaaac cagcctcttc ttttttattt ctatgcttct gctgctgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgcca tgaggagcca
    12301 gtaatttgat cccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcagctc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcagagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccggcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagcaat tttttaccat acggaatgat ttcttctaca gattggaagg ttgtattgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaat ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagccggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cccaaaccgt taatttcagt ttcagtatct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttca tggctaaacg tttaacagct gcctcttctg ggtccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattaaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctacgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt ttttctactc gcaatataaa tcagtcaaaa
    15841 acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcaa aaagactggt
    15901 tttttatttt tttgtctatc atcaagccgt tttatcgttt tttattttcg cttcttgttt
    15961 ttttcatata aacagaagca ggttttcttt ccttatgaag aagataataa taaagtccca
    16021 tcccgccaaa catgaccaaa aaaccgatga ctgatcgaat aggaaaatta ggaacaaaaa
    16081 agagacagac ggataataat gatgccggaa ccattattcg ataatagagt tgtttatcca
    16141 ttttcttcac ctcatatttt ttgtaaagta atcaattgtt ctaattctcg gtaatctaaa
    16201 aattgcacac gcagatgctg ctcacctttc ttgacatcga attggatact tcgccgcttt
    16261 ttctttattc gtttgaaggt aacatcagag agtctatgtt tgatttcctt taacggtaat
    16321 tctgacaggg attctgaagg aacatagatt cttaaggata aaagatactc ttgatatttt
    16381 tcatctgtta cctctacttc tagatattcc ctaatataat aacaataagc ttcgattcca
    16441 ttatccaaag caacagtcgg atcgataact ttatagcaca tcatgcgcga gcgattctcg
    16501 tcaacccaag gaaaatacgc ctgagcattt agtgttccgc gaacacgcct ttctttataa
    16561 tctccttgtt gaatatcata actttttgtt acaaaaaatg aagcattctt tttaggaaca
    16621 ggttgcattt gtgcattctg atactggaca gattggatcg tatggtcagt gtaaatgtat
    16681 tcaacgaatg gcggtttcaa taaaccgaca ccattgtccc aagcaagatg atcaaaccca
    16741 agctggtccc taaaatagga ttgaaacatt tgattgtttg gcttttgcca gttaaagggg
    16801 tctcgataaa gctggataca tatgacttcg ttttctaatt ccttcacttt ttctacttgt
    16861 tggatgtcga gaaaaatcgg tttagggatg atttgatgat aatagcgact ataatacatt
    16921 tcccaacacg aagtgaaaca taaaccttgg taaaaaagat cgtatcctgg aaattggttg
    16981 caatcgacta ctacttcccc gttttgttgt ttcattttag gtaaatgttc gatttcatcc
    17041 tttttctcaa acaatagccg ttgagagatc tgttttgtat tgtgatagat aaactcgcta
    17101 taagctcgca tataggcgaa caccccatga gcaatcattt ttttctgaat gaagtctcga
    17161 atcaaaaatt gcttttcttt gaaaacagag atagacaata cattttttat caataattga
    17221 ttatcttcca agcgaaaact acattcgttc tcgtcatttc ctacaactaa ccaactttcg
    17281 tctcttaccg atgctagcat tattttttct tgaagctttc gaagatcata cggatccgct
    17341 tgttgtgtga aagagcggat aaatgttggc tggatcaaca gacgtgtcaa aatagcccaa
    17401 tcgcttattt cctgtttttt ttcttcttca tttaagtctg tgagctggat agtcatcaat
    17461 aatttatctt cgccaatcat tttctcacct ctttaaaatc tttttataat ttaattataa
    17521 ccaacgataa agaaaaacta aaactttatg tcttagaaac caatttggac gattacatga
    17581 tagactaagt aaaaagaaac aaagaggtgt ttacaatggg gttgaaattt gaacgtacag
    17641 ataatttaga taaacttttc gaatcatttg ctgtcgatcc ggaaaaaaca gttgcaccaa
    17701 aagaagagat cgatcgattt ctaaaaccac agccacaaaa tgaaaagaaa aaagacaaga
    17761 aacaaaagta agttaagctc aaaaaaagac ttaccagcca aacttttatg aaagagtttg
    17821 actggtaagt cttttttatt ctttttatga ttcaattgct tgttcgattg ctgcccacgc
    17881 ttcttctttt ccctgttttg taacggaaga aaagagaata aacgtgtcat tgcgatcaaa
    17941 atccaacgct tttttgattt cgctctcatg cttgttccat ttaccacgag ggatcttatc
    18001 tgccttagtg gcaacaacaa ttacagggat atcataatac ttcaaaaatt gatacatctg
    18061 gatatcttct tgagaaggct tatgacgcaa atctactaaa gaaacgaccg cacgtagctg
    18121 gtctctagtc gttaaatatg tctcgatcat ctttccccat tttgctcgct cagttttgga
    18181 tacttttgca tacccatatc ccggaacatc gacaaaatgt aatgcgttct cgatcaaata
    18241 aaaattcaat gtctgggtct ttcctggctt accggaagta cgggctaaat tctttcgatc
    18301 aatcaatgtg ttgataaaag aagatttccc tacatttgat cgtcctgcca gagctatttc
    18361 tggcaattgt gtatctggat attgttttgg tgccactgca cttatgataa tttcagcttg
    18421 atggactttc atggtctctc tccttaacaa ggtgatgaat aaagcaagcg gctgtcgcaa
    18481 aagccaaaat gacttttacg gcagcctctt ttcatcaacc cgcttttttc tctgaatctt
    18541 tatagattac tgttggctca ccagacaatt cagctgcttc ttttgtgata atcacttttt
    18601 cgatgttttc atttgaaggg atatcaaaca tcacatccat catgatttct tcaatgatgg
    18661 aacgaagtcc tcgagcaccg gtattgcgtt cgattgcttt tttcgcaatg gctctcaacg
    18721 cttcaggttc gaattcaagt tcggtattat ctagagaaag aagtttttga tattgtttga
    18781 ctagcgcatt tttcggctca gtcaagatcc gtaccagatc atctgttgtc aatttctcta
    18841 aagctgccat aacaggtaat cgtccgataa actcagggat caatccgaat tttagtaaat
    18901 cttcaggaat gatatgctgc atgacacttt cgtcttcaga aagtttttgg ttttgtgtac
    18961 caaatccaat cgtcttttcg cccatacgat tcttcacaat cgtttcgatg ccatcaaatg
    19021 caccacctac gatgaacaat atgttcgtcg tatcaatctg aataaattct tgatgtggat
    19081 gtttacgtcc tccctgaggt ggcacgcttg caacagttcc ttctagaatt ttcagcaagg
    19141 cttgttggac accttcgccg gaaacgtcac gagtaataga tacattttcg cttttacgcg
    19201 caattttatc gatctcatca atatagatga tccctttttc tgcacgttcc acattgtaat
    19261 cagcagattg aagcaattta agcaaaatgt tttctacatc ttcccccaca taaccagctt
    19321 ctgtcaaact tgtcgcatct gcgattgcaa aaggtacatt caatgttttt gctaatgttt
    19381 gtgctaaaaa tgttttacca gaccctgtag gtccgatcaa gcagatattg cttttttgca
    19441 gttcaacatc atctgtgttt tcttgtgctt gctgattgac acgtttatag tgattgtata
    19501 cggcaacagc taacgagcgt tttgcgcgat cttgtccaat cacgtagtta ttcaatactt
    19561 caagaatttc tagtggttta gggacttcag tgaattcgcg tacagcttct tcataaaatt
    19621 cttcatcaat gatttcttta cataggtcga tacattcatt acagatgtag acccctggac
    19681 cagctacgat ctttttcact tcttcttggg ttttcccgca aaaagaacag cgtacggtct
    19741 cattgctact tggattgtca tacatggtct tcacccctta acttgcaaaa agtatgcaag
    19801 aaactatcaa agactttaca gtcttttact atcataacat agtttagtaa aaagaaaaag
    19861 tgtttaacaa aggatcaact gacctatacc tttgcatttt ttactcttaa caaaaaataa
    19921 ggaactgtga caaaatcgtg tcacagtccc ttatttcgaa taaacaattt tctagaagta
    19981 gcttcttcag aaatgattta ttacttgatt tctattcttt cgcttctgtc gctgtacctg
    20041 tgatcaattc gattgctttt ttcattgtca catcatgttc cagcatatct tctgtcaaca
    20101 cgcgtttgat ttgttcgatt ggcatgttgt atgtttctga taattcttgg atttctgcat
    20161 tgatttcttc ttctgttgct tgaagatttt cagcagctgc aattgcttcg atcacaagat
    20221 ttgttcttgt gcgcatttct gcttcacctt cgaattgttt atgcaagtct tcttctgtag
    20281 aaccagttaa ttgatagtac atttcaggag agataccttg acgttgcatg ttgttcaaga
    20341 attcgtccat tgaacgatgt acttcatcat gaaccatcac gtgtggcaat tcaacgattt
    20401 ctgcgttttc aacagccata cggatcgctg cttcatcttt tgcatcgtcc gctgcttttt
    20461 cttttgtttc tactaattct ttgcgatatt tttctttcaa ttcgtctaat gttgatactt
    20521 cgtcatcaac atcttttgcg aattcgtcat ctaattcagg cacttctttt gcttttactt
    20581 catggattgt cactttgaag actgcttctt ttccagcaag atcttctgct tgataatctt
    20641 ctgggaaagt tacatttaca tctagacttt cacctgattt atgaccaact aattgatctt
    20701 caaaacctgg aatgaatgag cctgaaccta attcaagtga gtaattttcg ccttttccgc
    20761 catcaaacgc ttcgccatct acaaagcctt cgaagtcgat tactactgta tcgccatttt
    20821 cagctgcagc atcttctttg ataactaatt cagcttgtgc ttcttgttca cgtttgatac
    20881 gtgcatccac gtcagcatct gttacttcac ggtcttgttt ttcaacaact agatctttat
    20941 attgtcctaa ttttacttct ggtttcactg ttacttcagc agtgattacc cagtcttgac
    21001 ctttttccat gctttcaaca tcgatttttg gttgagaaac aggatcgatg ccagcttctt
    21061 tgacagcagc ttcataagct tctggtaata cagcattcaa tgcatcttca tataaagctt
    21121 cttcaccgta catacggttg aatacttgac gagaaacttt ccctttacgg aatccaggta
    21181 cgttcaagct ttttttcact ttattaaacg cagttgttaa tcctttttgg atttcgtctt
    21241 gcgcaatcgt aaattttaaa acaccatcat tcgtgccttt tttttcaaat gttgcagtca
    21301 tttaattccc tccgagttta gaaattcatt ttatacatca aaaacgtttc atgcatacct
    21361 ttaaatagta cactaaccct ttcaaattgt aaacatattg aatggataga atccttgttt
    21421 ttcataacat ttttatcttt ttcactgata aaatctaatg gaagaagatc atttgctgca
    21481 aaattttttc tctttttttt aaaaaatctt gtttttcatc ttctgtacct tcatacatcg
    21541 tttttcctgt acacgcctct acagtcagtt gaaaccagcg cttcgggtct tcgatgtatg
    21601 tatcatagac aggataaaca attgcagatt gcaacgtgaa ttcttcattt aacacggaac
    21661 taagcagcgc atcttcatta ccgtaacgat ctgaaaatac acataattgc tggtaacttg
    21721 cctgctgctg cggctttggc agatcttttg gtatgacttt gactaacttt tcttcaatcg
    21781 tcaaatagaa aacaggatgg cattcgttca attgcctaag tgtctccagt aattttgccc
    21841 ttgctaaaat atggacctct ggcgatacaa taagtttact cgctgtttgg ataaactcat
    21901 gataaggaag ctgttcgatc tcatcaatag acgcaagctg acgtgcaggt tccaacgaag
    21961 gaagttcctc cgatttattg tggagtgcct tgatcttctg cctctccatt tgtccggata
    22021 attcttctgc aaattggatt ttttcaatga cccgctgctc gaaagcttca taaaaatctg
    22081 tacttgctat caattttcta gcatataaaa aatcttgtgt actgattaac aagtcaaaat
    22141 aaaattcagc aaactcctca ttttgaagat actgtttttc atgcaagagt gcttgcttca
    22201 atgcttcttt tttctcgtcc aactcgtaaa gacaaaaaac aagcaggcta ttagcctcaa
    22261 agctttcttt caactcgtaa gctcgttgaa ataattcttt tgcctgctga tattgctggt
    22321 ttgctagtgc ttgttgtgct actcgcatta aattctcata tttttctgga aaatcaatcg
    22381 gcttcacgca gtgtcctcct cttgctcatg ttcagctcat gttcaatagt agcaaaacaa
    22441 aaaaagcatg acaattaaaa ataactgaag caccctctaa aagctagatt ttagatctga
    22501 cttttagaag gtgctttcaa ctgttactgc tttttattgc tcatcagctg ttgacttcct
    22561 ggttgaatgc tttgatcaga ttgatcgtaa caacacttgt attagtgaaa aaatcgccta
    22621 gatgttgctt tttctgatta aagattgctg gaagataccc taacatcagc aaaggaaatt
    22681 ttagaatgaa ccggcaagca ccttctctaa ctaaaacggt tgaccagcta agttcttctt
    22741 catcaaagct gatgactcga ataccaaaga tcattttccc aatcgtttga ccatgattga
    22801 atttcgtcaa taaaataaaa tacgctagat aaataagtaa ggctgtgaat ccgtagatac
    22861 tcagataaga atctgaaaaa ttaagccaac ctagattttt ggcaatacca agtgttaaac
    22921 gcgtaatagc tccgatacac aaaagatcga ccaagaaagc aaacattcgg ataaaaaaac
    22981 ctgcatagaa atacttagga aaatcatttg cttttggcgg ttcatttctt gtgtccgtat
    23041 actttttcca tttacgttgt ttctttttga tttcttcggg cgtcaaaggc tctctttcaa
    23101 aagagctcag taccttttgg gaaaagttgg agtcaggtcc tacagcaggc tgctgttgct
    23161 tatctttttc ttgttcctta ttttcatttg aaagctctga catgcttact cacctccgta
    23221 ataatacatt gcttttggtg attccgctgt ccctagattc tcaagaacgg ataagatctg
    23281 actagtctca gaaggcttca atccctggaa ttctgctagt ttacttccta accatgtact
    23341 agcaaaccca gatggcgtgg aagaatattc tactagttcc gcatcttcaa gctgttgttc
    23401 ctttcgcata gcttttaaag catcttcagg aaatcccaat tcatcgacta aacctacttc
    23461 ttttgcttgg acaccatcat aaatccttcc atcagcaatt tttttgactt cttcttcaga
    23521 tttattccgt ccctcgctga cgattgctac aaaacgacta tatgcgctgt cgatatatgc
    23581 ttgaagcacc gcttcttctt cttttgtctc tggtcgcgtt cctgaaccca tatctttcaa
    23641 cgctccgctt ttaactgttg tatcttccac acccagtttt tctagaagtc ctgaataatt
    23701 caatcctgac atgatcacac caattgagcc ggtcactgtt tcttctgttg caaagatttt
    23761 atctgcttga gcagaaatat agtagccacc gctagctgcc atgtttttca tgctgacata
    23821 cattgggata tcatgttctt tacgaatcgt atccaatttc ttagcgattt ccgcactttc
    23881 gtagacaccg ccaccaggag aatttacttc taaaaagata cctttgactg ttggatcttc
    23941 ttcaatcgcc ttcagttgtt ccataaaatt tttatgattg taaccttcag aagcaaataa
    24001 acctgaagcc cctcccgcag caatcgttcc atctactgat aattttacga ttttttgcgt
    24061 actgtctccc tcttccaaca catttgaagc tagttcttca tccccatata accaactgtt
    24121 caatccctca atttgatcgt tttcctgagg cttagtcaaa ctagctgaaa ctaacgaaac
    24181 cacaaataat cctgcagcaa tcagaactgc tgcccaacgt ctcttattca ttctttctcc
    24241 tttctaatac aagtttttta tgacgatcac agtttctgac tgctcagaag tgtcgaaccg
    24301 ctctgctttt tcttcccgcg catacccgga aaacgactct tttgtcaatt catcttctac
    24361 cgtaaactca cgagcttcag caacttccaa ggcatcaggt gcgatttctt ggatgctatc
    24421 ccagccagta tctaaaaata ttttcgtatc atctgataca ccagctgatg cttcgaattt
    24481 ggacaattgt ttttttaaca ttcccagtgt catgatctca atttttgaat ccatttttac
    24541 tcctcctcta tccgattact ttctattgta ccgaactcct gaaaaagatg atagataaaa
    24601 acccattatt ttttgacata aaaagagcat ttgacaaagc atttgatata taccaattta
    24661 ttttttattc tttttttgtt gacaagaaca ttcgcaaaag gttatgattt taaatgtttc
    24721 cagataaaaa acaaaagagg aggatagtta taccttttag gatagcgcca ggtctgtgta
    24781 aacagatgac gaggagaggg tttatcgaaa gattcggcgg atggccctag ggactgcact
    24841 ctacagaaaa tccacaaaaa atatctgcaa agataaaaca aaaggatttt cctgcagctg
    24901 gaagcattca tacgaattca ttatagaaag taggatgttt tttatgtgcg gaattgttgg
    24961 aatgattgga ttaaaaaatg taacaccagg attgattgac ggtctagaaa aattagaata
    25021 ccgtggttat gattcagctg gtatctttgt ctcagatggc acaactgatt atctggtcaa
    25081 agcacaaggc agaattcaaa atttgaaaaa caaaataaat attgatacta caggaaatat
    25141 aggtattggt catacacgct gggcaactca cgggcaacct tctgaagaaa acgcccatcc
    25201 acatacttcc caaagtggac gcttcgtgct cgttcacaat ggcgtaatcg aaaactttga
    25261 agaacttaaa attgcttatt tagcaaatga ccactttatt ggtgagaccg atactgagat
    25321 tatcgctcat ctaattgaaa ctttcgcgaa ggatactaca acgcaagagg cgtttttaaa
    25381 agctttgcgg gtcatcaaag gttcttacgc tttcgctttg attgatcgga cagcacctga
    25441 tgttatttac gtcgctaaaa ataaaagtcc actattaatc ggactcggcg atggattcaa
    25501 tgtgattgcc agcgatgcaa tggctatgct tgctcatacg aaggaattcg ttgagattga
    25561 agatgaagag atggttaccg tcacatcaga aaaagttatt attcaaaatt ttgcaggtga
    25621 catcgtagaa cgttcttcat tcgaagcaca agtagattct tcagatatcg agaaaggtac
    25681 ctatcctttt tatatgctga aagaaatcga cgaacagcct attatcatgc gtcgtatcgc
    25741 tcaaaaatac gtaacagaag acaatcgtgt ctctcttgat caaaagctgg tcgatacatt
    25801 gtctgatagc gatcgaatct atatcgttgc ttgcggaact agttaccatg cggggttagc
    25861 aggtaaacag actctggaaa aattgacaca gatccctgtt gaggttcatt tagccagtga
    25921 atttggctat aacacacctc ttctttcaaa aaaaccattc tttattttct tgagtcaaag
    25981 tggcgaaaca gcagatagcc gccaagttct ggtaaaaatc aaccgtctcg gctacccttc
    26041 acttacgatt acaaatgttg ctggttctac tctttcgcgg gaagcatcct ttacactgct
    26101 attgcatgct ggacccgaaa tcgcagtagc atcaacgaaa gcttatactg cacaaatagc
    26161 tgtactaact ttattagcca aagctatcgg tgataaaaaa gaatttgaaa cttctcttgt
    26221 attcgatatc actcatgaat tgagtttagt tgctaatgcg atggaatcgg tcatcgccca
    26281 aaaagattat ttagaagagt tggcagaagt atatctcagc gatacaagaa atgcctttta
    26341 cattggacgt ggcgctgact acaacgtttc tcttgaagct gctttgaaat tgaaagaaat
    26401 cagttatatc caggcagaag gttttgccgc tggcgaattg aaacatggaa cgattgcttt
    26461 gatcgaagaa ggaacaccag tgattggaat catcagtgaa gaagtgactg gtgcgcatac
    26521 tcgaggaaac ctgaaagaag tcgaaagccg tggcgctaaa acactagtta ttgtctcaga
    26581 gggattagaa aaggaaagtg accagctggt gctaccaact gtccaccctt atctaactac
    26641 tctaaccaca gtcgtaccga ctcaattact tgcatattac gcaacgctat tacggggatt
    26701 cgacgttgac aaaccacgta atttagcgaa atcagtgaca gtagaataat aaaagaacaa
    26761 ggaccagtgt cagacatctg acactggtcc ttgttctttt attttttatg aaaatatctc
    26821 ttaatccagt ccgatttctt ctttgactac tgccgcgatt ttatcgacat agtaattaac
    26881 tttctcatca gtaggcgctt ctgccatcac tcgaagtaat ggttctgtac cggaaggacg
    26941 aactaggatt cttccttctg atcccatttc agcttctgcc tcttcaatca cttttttgat
    27001 tgccggaaca tccatcgcac cgtttttatc acttactcgg atattcacta atttttgcgg
    27061 atagattgtt acctctgctg caagctctga taatttctta cctgtctgtt tcatgatatt
    27121 cagtaactga attcctgaaa gcatcccatc tccagttgtg ttaaaatcta agaaaatcat
    27181 gtgcccagac tgctcgccgc caaagttata atcgtttttg cgcatttctt ctacaacgta
    27241 acgatcacca acttgtgtaa cgacatcttt caaaccgatt ccttccaccg ctttgtggaa
    27301 tcctaagtta ctcatgacag tcgtaacgat cgtatctttt ttcaatcgat ttttttctgc
    27361 caagtattta gcacagataa acatgatttt atcaccgtcg acaatgtttc caagttcatc
    27421 caccgcaata atacgatcgc catcgccatc gaaagctagt ccagcatctg ctcctttttc
    27481 aacgaccatc tcagctaaac gttctggatg ggtagagcct acgccatcat tgatatttaa
    27541 cccgtctgga tttgtgccca tggtataaaa atctgtttca agatcggcaa acaaacgatt
    27601 aacacttgtt gaagttgctc cgtttgctgc atcgacacaa atggtcaaac cggaaaggtc
    27661 accgttgatc gtttgtacaa ggaattgaga atatttcaat aagccttctg gaaattcttc
    27721 tacagttccc aatccttcag ctgatggacg aggcaattca tccacttcag catctaataa
    27781 tgcttcaatt tctagctctt ggtcatctac tagtttaaaa ccatctgcac cgaaaaattt
    27841 gattccatta tcttgtgctg gattatgaga agcagaaatc atgacccctg cgcttgcttt
    27901 ttgcaaacgt gtcagataag caacacctgg tgtagagatc acacctagct ggaacacttc
    27961 gatacccacc gataaaagtc ctgaaatcag cgcttcttct aacatttgac ctgagattct
    28021 tgtatcccgc cctactaaca cgcgtgggcg ttttccttct tcttcatgct ggcttaggac
    28081 ataacctccg caccgtccca gcttaaatgc aagttctggc gtaagttcct tgttggcttc
    28141 accgcgtacg ccgtctgttc caaaatattt acccatagtt gtaaaattac tcctttaact
    28201 taattttctg aggaatttgt tctgctcatt tgtgttgatt cgcttgtttc tgatgtttct
    28261 tctgtacttg tggtcgagct gcttgtccca gattccgaac tggaagaaat ggttgttgtc
    28321 gatggttccg ttcctgaatc agcagatgaa atacttctac tcgtacttgt attttcttgt
    28381 gtccgactgc tcgtcgaagc agagctactg cttgacgttt gcaacacggg tgtaatctcc
    28441 acactaactg attcgggctt gatacttaac ccctcttcta ctggtatttc aattttacga
    28501 gttgttcgat gattgatacc tgagacattg atcggtacag caatactgtc accgatcgat
    28561 gcaagcaaat cgctagagcc gcttacttct gcttccagtt ttgaaaaact gaaatcatag
    28621 ctttctaccc cggcagctgg tgttccttgc tgtatcccat aaagtcgtac cgacttgctt
    28681 ggcttgatcg aatcagctct cactgttact tgcactggat cagagacgat acttaatact
    28741 tcccctgctg cattcagcgc ctgtattggg acctcactat taatcccatc atctgtgacc
    28801 atcgaagcgt ccacatcagc tactactcgt ttgatttctg ccaatgtttt gtcacctgta
    28861 gtgatagaga cttttttagg actaacggaa gtatcatcaa attcatatcc agatggtgtg
    28921 aacgttgaag gaatcaacgt ttccacatcg aaatctttta ttaccttttt ctcaaccgtt
    28981 actgtaatag tcgatggttc gatctttgct gtgacagcac tgctcaaatt ttgaattttt
    29041 agcggaacct catgtgttcc ttcgcccagc ttagtgatat ctgctgtgac gcggaacatc
    29101 cgtgtatctt cgtttgcctc tgcattcaac tgaactcggt ttgcactgga aagtttgaca
    29161 gtcactgcat tttcatatcc atggatatag tagcgctttg aatcataaga aatcttgatt
    29221 ggcaccccag taactgtttg attatacgct tcatttccag aaagcgtatt tggaacggac
    29281 tggccatttg cattgaagta cagcgcaata ctgaacagca atgcaataaa actgtaaaga
    29341 atgcttgtcc gtttctcttt tgagatcatt tgcgatcccc tcccttagtc ataccgtcta
    29401 ggaaagattg aatcacgttt ttcttgtttt ttgttttctc ttcagcaatc agttctctac
    29461 gcaaaattgc caaatattct tgctgggata agccagccat cagttcatta tctaatgtaa
    29521 tgctgacatc cccagtttct tcagagacaa cgatggtaat cgcgtcgctg acttcgctta
    29581 taccgacagc tgcccggtgt cgagtcccga attcttttgg aatcaacata ctttctgata
    29641 gaggaagata ggcgcttgct actgcgatct ttccttcttt tacaataact gcaccgtcat
    29701 gcagcggtgt attaggaata aagatattga tcagcagctc tccagtgata tctgcatcta
    29761 atgcaattcc tgtctcaata tactcatcta atccagtatg ccgttcgata gtaattagcg
    29821 caccgatttt tcgcttggac atatattgga tggctttgtc aaaagacaag atcatcttct
    29881 catcttcctg ctgttcgctt tttgattgtt tgaaaagcga actgcgtcca agatgttcca
    29941 gtcctctccg gatttccggc tgaaaaatca cgatagcagc tatcaccccg tacgtaatga
    30001 cttggttcat cagccaagac aaggtgtgca gaccaatgat ttccgctacg attcggataa
    30061 caataaaaat agctacacct ttcagaagct gaatcgcttt tgtgcctcgc agcagttgga
    30121 tcagtttgta tacaagatac cagacaacca gaatatcaag tatgttcacc aaaaaattca
    30181 gagagaacaa gtcaccggaa aacatttgct gccaatagtc caaattaaaa agttccccga
    30241 tttgaaatga cataaaaagc ctctcttctc ttttgagatt tcctctctag tatatcatat
    30301 gttttaattt tagaggctga tctgctcgta tttttcttga gaaaaacgca taattttcag
    30361 gaaaattgca agtatgcgtt ttatttttct ctgcaataaa tatttttaaa cttcagagaa
    30421 ttattcatta tattttttct gttatttttc ctttttttct tttgtagctt gttccatttt
    30481 tttacgtcgt tctgctctgg cagctcttct ttcttctgct tcaaggtccg tcgttttttc
    30541 ttttctagtc ttaacgggta catcgataga ttcctctggt tcaagtcgtt tcttgatttt
    30601 ttctaattct cccgtcagtg cagcatagtt gtaaaaacgt gttttagcgt cttggatagt
    30661 cttttcaaat aaagcttttg caagatttgg atttgcgttc tctagcgagg aaaagcgaac
    30721 ttgtttcctc atgaaatcag gcatagcaga gaaatcaggt tttttgtagt ccaaagtcat
    30781 tgggttcttg tttttttcga ccattgcagg gttatatcga tataatgacc aatagccaga
    30841 agaaacagct tcttttgctt ctttaagcga ttctttcatt ccaccaagca aaccatgtgt
    30901 gatacaaggc gtataggcaa tgattaaaga aggtccagga tacttttctg cttcttcaaa
    30961 agcctttatc gtctgcattt gattggcacc tgaagcaatt tgcgccacat aaacatttcc
    31021 atatgtcatc gccatcattc ctagatcttt ctttgaagca tactttccac tagccgaaaa
    31081 tttagcgatt gccgaagctg gtgttgcttt tgaggtttga ccgccagtat tggaatagac
    31141 ttcgttatcc aaaacaagaa tattcacgtc tgctccgctg gcaagtacat gatcgatccc
    31201 gccaaaacca atgtcgtaag cccatccgtc tccaccaatc atccattggc tgttttttac
    31261 gaatagatcc tttctcttgt atatatcctc cagttcaggg acaattgatc gttcgctttc
    31321 gatcgcagct tgcagttttg ctgctctttg ctgcgttcct tcgctctcaa acatgtgtac
    31381 caaccagtct tccatcaatt ctcgtagttc ttctgatact tttggcaata cttctttgat
    31441 tgaacttgcc aaagcctcgc gacgagtctg tgccgctaga agcataccat aaccaaattc
    31501 agcattatct tccaataaag aatttgacca tgcaggccct tgaccacatt cattggtcgt
    31561 ataaggagaa gcaggagaag caccgcccca aatagaagaa cagcctgtcg cattcgcgat
    31621 catcattcga tcaccgaaca tttgtgtcaa taattttaca taaggtgttt cgccacagcc
    31681 tgagcaagcg cccgagaatt caagaagcgg ttgattgaat tgtgtagaca agacagtatt
    31741 ctttcctttg actggatttg ctttttgtct taatgtcatt gcaaaagccc aattcacagc
    31801 ttgttctttc tgttcttcat aaggcttcat cactaaagct ttttcttttg cgggacaagc
    31861 ctctacacac agaccacaac ctgtacagtc ttccactgaa acttggatac ggtattttac
    31921 gccgtctgcg cctcgcattt ctctcacgat aaatccttct ggcgcttctt ccatttcctc
    31981 ttcatctgct aaaaatggac ggatggcagc atgaggacaa acaaaggcac attcattaca
    32041 catcgtacag cgatcactaa tccactcagg tacttctaaa gcgacgcccc gcttttccat
    32101 agaagccgtt cctaaaggca tttcgccagt tgtcattcca tttgctacaa gtgtaccaac
    32161 agagagttga tttccttcct gtgcattcac tggttcaagt atttcttgta tataaccagt
    32221 gttcctttta ttgatttttt caggcatctc caacgttctc caatggtctg ggatcgcgac
    32281 ttgatgaagc agttcggtcg tccgatcgat tgcttgtatg tttttttcta cgatagccat
    32341 tgatttatgt ccatagcttt tcagggcttc ttcttttagc agcggaagca cctcatcaaa
    32401 aggaatgatg tcagctaatt taaaaaaagc cgtttccatt gccgtattga tccggcgtcc
    32461 caaaccgact tcttgggcca atctcatggc attgatcgta tagaatctaa tgttattttc
    32521 agccaagtaa cgttttaatt ttaagggcag atgcgtttct aattattcgt cgctccaaag
    32581 agtattcaat aaaaaaatgc cacctggctt caatcctttg accaagtcat agctatgcag
    32641 gtaagctggt gtgtgacagg caacaaggtc cgcatgctcg acaagatacg ctgaacgtat
    32701 cggcgtatct ccaaaacgaa gatgagagac agtcagaccg ccagattttt tcgaatcata
    32761 ataaaaatag ccttgcgcat atttatcggt gtgatctcca ataatcttga tagctgattt
    32821 atttgctcca actgttccgt cagatccaaa tccccaaaac ttggcctgga atgtttgcgg
    32881 ttctgtcaaa tccagacttt cctttggttc caaagattga taagtcacat catccacgat
    32941 ccctatggta aaccgttttt tgcgtatgct tgggtctttt ttcaattcat caaaaactgc
    33001 tgagatttgg tcgggagtca catctttaga ccccaatccg tagcgaccac caataacagc
    33061 cgggcgaagt tcagaatcat ataacgcact ttgaacgtct aataagagag gttctccacc
    33121 tgcccctggt tctttacttc gatcaagaac agcaattgtt tttactgtat gaggcaactt
    33181 ttgcacgaat gtttccactg gaaaaggacg ataaagatgg atattcaaaa aacctgtttt
    33241 ccgtccgttt gcattcaagt aatcaatcgt ttgttcaatc gtctgtgcaa ctgatcccat
    33301 agaaacaatg acttcttccg catcgtctgc tccgtaataa gtcaccaagt cataagaagt
    33361 tcctcgtaat cggttgatct ctttcatata tttttgaacg atttcaggta atttatcata
    33421 ataggggttg atcgtttccc gttgttgaaa atggatgtct ggattctggt tcgtaccact
    33481 aacagtcgga tgattgggat tcatccctcg tttacgaaat gaagctaatt tctcctgatt
    33541 gatcagagga gctaattctt catattccaa aacttcgatt ttttgaatct cgtgactcgt
    33601 acggaatcca tcaaagaaat tcaaaaaagg tacttctcct tcaatagaag caagatgggc
    33661 aacaggtgct aaatccatca cttcttgtac actgctttca gctaacatag caaatcctgt
    33721 ttgtcttgca gccatcacgt ctccgtgatc tccgaaaata ttcaacgcat ttgtcgtcac
    33781 tgcccgacta gcaacatgga aaacacttgg cagtagttcc ccagcaatct tgtacatatt
    33841 cggtatcatc aaaagcagcc cttgagaagc ggtatatgta gttgtcagtg caccagtttt
    33901 caatgatcca tggaccacac cagctgctcc tgcttcggat tgcatttcta ctacttttac
    33961 tggttgaccg aatacatttt cttttcctgc tgctgaccat tgatctacta attcagccat
    34021 ggtcgaactg ggtgttattg gatatattgc agcaatctcg gtaaatgcat aagaaatata
    34081 tgcggctgcc gtattgccat ccattgtttt catttttcgc atgattcatc atccttgttc
    34141 atattctctc ttattatctg ttccattata acgtaaaaca ttccattttt tcttttattt
    34201 ataaaaatat ttcacatatg tctatttgaa ttggtcatct aatttttaga caaacagtct
    34261 aaatcgttgc ctttttttgt cagacatgta taaactataa gcgaaagagg tgttttaaca
    34321 tggatatatc agtgattgat gcaacgaaaa caaatacaca aaaaggaatc ctttatggtt
    34381 catcgaatgc tcctaaaaaa atggtcgaat tcatcaacct atcttgtccg tattgccgac
    34441 aatggtttga agaatcttat gagctattag aagaagcagt ccaaagcggg caattgcagc
    34501 gagtgatcaa attgttcgat aaagaaaaag aaagcttgca gcgaggaaac gtgatgcacc
    34561 ggtatctcac aatcagcgat ggtcaaaaag cgatcaaaga gatcaaacaa atattcgata
    34621 ctcaagacga gtggaaacat ctttctctcc aagaagtagc ggattttgca gtagataaac
    34681 taaagctgac agaacaaaaa gacgaacagc tgagccaagc tgtcatcaac gaagctgagc
    34741 aagcacatat tcgatttgtt ccgactgtca tcttgggtaa agaaatcttt gatgaatcta
    34801 tttcaataga ggaattaaaa gaattgatcc aaacaaaata aaaaaataag gagcgtagga
    34861 caaaagtaaa gaacactttt gtctcacgct ctaaacacct ataaacggcg gaagcagaag
    34921 caactccttc ttatttcttg gagttaaacg cttctgtccc agcctcctta ttccgaataa
    34981 atggtgtcca aaagcttgca tctgccagat tatctcattt cttatgacaa gctgagcaaa
    35041 ttatctccac caaataattt attcatattt ttgacttgcg catactaact aattcttaaa
    35101 ttttcataca aaatttctca aattccctta acgagtaata tttatgtacg gtgattcaca
    35161 ctagtatccc aatcactttt taagttatcc cgatttcaat tctacttcaa aaatagttgc
    35221 ttttatgtat gctgttagtt gattagatgg tcgtttgttg agctaggtcg tttattttaa
    35281 acaagttgtt tctttagctt atacctatct cgtctaccaa acgcaagatg gcaaacacaa
    35341 atgcatacgc atctgtattt gccactttga ttcgtataaa gctaacaact cctaactcct
    35401 ctcatttaat agttgctctc ttttttattc agatacattt ttgctgatca cagcctagta
    35461 tttcaatggt tttgaacact aaaccaaatt gttccattac aacattcact ggctgatgaa
    35521 atggattaat agatttcgat tattcaaagc gattttctat tggtggattg tgttcccaac
    35581 taacaagacg gattccaaag tcattatata actcttcaag agttaaccct ttcctgttcc
    35641 caatagatgt atctctccca cgctcgatat aaaatatgga gtttcgaatg ccaagacctg
    35701 gcaaatcttt ccttgttaca gttacactgc tactaacttc cccatataaa atccagctcc
    35761 tccaaaatga taatatattt ttcgaccatc atctaactca tgtaacaata attgttgtgc
    35821 cctcttgtca ctcgccaaat gcaaacgcca cgtgcgatac catgccatgc tacctgcatc
    35881 tgcagaaaat ccatcaaata atgcaacaat tgtatcttct atcacaatcc tttccccatc
    35941 tacttcatat tctaatctaa aatcaaactc ccctcgggta atcgcaggtc ttggtggctt
    36001 aggacccaat attattctac ttaaaaccgc attcgttccc agatgaaaca cagctataac
    36061 aacaataatg attaatattt tgtttctatt tgataaattt ctcattattc gcaaatcacc
    36121 tctacattct cacatactag ttcgtaatac tcctttaata tcaaaatatc tactgatcgt
    36181 aataatattc gctatgaatt cctactactc gtataagaaa ggccaaatca tcagctttta
    36241 cttgctgatg atttggcttg tttctattta tcgtaattgt tttctaatca ccaaaattgt
    36301 taacaatcgg ttcagatatt tcccaattca ctagccttat attatattga tttaacaatt
    36361 cttcttcatt tattgtattt tgatcaagcg gaccaaattg ttctaataat tctttgtcat
    36421 gtaaaatatc ccttctagta atgcctcttt ccctttcaat gaaagaagca ctaggaaaag
    36481 tatgattata accattcaac ctatctccca tgtaataatt agcaggtcct ggaaaataaa
    36541 atatttgctt attatcacta acttctaaca gcaacactaa atcttctccg ctggctaaac
    36601 gacttgtcca tctacgatat ctccctatgc cttcgttcat tccaaatcca tcaaattcaa
    36661 ctattaacgt gtcttctatc acatgtaatt cttcattcaa ttcatactca agtctaaaag
    36721 gaaattctcc atatgtaatc tctggcaatg gtggtgctgg agttctccaa ataaatacaa
    36781 taactgtgaa tagaaaaaat ccaacgatcg atataaatgt tatgagacct gctcgaagta
    36841 aaacccgata taataaaaat gctgtatttc gctttgctat atctagaaaa ataaaaagta
    36901 ctgcaatcat aatgatagta aaagttgtaa aaaaacgcat ttttgccctc cctttataaa
    36961 catcgattct caaattctaa accaaatctt ggtagttcaa gttcaggtat aacgtattcc
    37021 aaccatacat ctcaattact atttatgaac ttatgattat cactgaataa atcactccat
    37081 ccccttgtta aaacttctac attaattaca cctggttgtt ttcgactagc taatccaatg
    37141 aacttatcaa ccatccttat ccaccaacca cgagaatctt gaccagaagt aagaagagca
    37201 gttctcaagg atgatcgagc agttccttcc agttttttaa ttaatcaggc cagttataga
    37261 taaatacttg atcttatcta tatcaggact tctatattaa gcaaaaattt atgtacaaaa
    37321 ctaaaatctt cattcagttg tctccccgct ttttatacga ttgtattaaa aacaatagaa
    37381 gcctttccaa attatgttac tccagtaaag atggaattat agtttgacgg aagaaaagca
    37441 agtataactt ttaaaaaaaa ctgatacatt cgcatctcct gcttcatgta tcagtttttt
    37501 ctttggatta aactgcttct tcacgcagtt tttccgcaaa ttcatttatt ttgcgaattc
    37561 ggtgattaat tcccgatttt gatatcgcac ctgagggaat catttcccct aattctttca
    37621 agctgacctc tggatgttga agccgaagtt ctgcgatttc ctgaagtttt tcaggaaggg
    37681 catgtagacc gacctttgct tcgatcaatt cgatgttttc gatttgttta gaagccgcat
    37741 caatcgtctt gttcagattt gctgtctcac aattgactag acgattgacc gaatttctca
    37801 tatcccgtac gattcgaaca tcttcaaatc ggagcattga gttggttgca ccaatcaatg
    37861 ttaagaaatc agcaattttt tctgctcctt ttaaataaga aatatagcca ttgcgccgtt
    37921 caagcgttcg agcatgtaga ccgtaataat ttaacatatc gcaaatgtct tggttatgct
    37981 gttcataaat agaaaatatt tccaaatgat aacggctcgt ttccggatta ttgactgaac
    38041 ctgaggccat aaaagcacca cgtaagtaag aacgcatttt ttgcgaattt cccataatct
    38101 cgtccgaaac gtgtccattg aacatcatac catccatgat atcaagatcc atcaacacat
    38161 cttttgtgcc ttgcttcaac cggacgatat agacattgtt cttttttaat ttcatttttt
    38221 tacgaacgag tagctcactt cgtacctgat aatgatcttt caaaagagaa tagatacgtc
    38281 tggcaattgc agcattttct gtttggacgt tcaaaataaa ctgctggttc acaataccta
    38341 aagagccgtt cattcgcagc aaagcagcta attccgcttt cgcatgttct cgatgaactt
    38401 ccaaaccagt tagttctttc ttcacatctg cagcaaatga catcctattc tcctcctttc
    38461 tccaacaata aattgcttaa tattttgaac cgaaaactaa acggaacaat tcttctacta
    38521 ctttgtctcc atcgtggaag acaccaccat ctttcaattc caaaaagtcg gtggaaatca
    38581 ctcgacatcc ttcatttctc aatccgttga agtcgtgtgc gacttgtact agatactcat
    38641 catagatctc tgggtccata taatcttcag gcactttttc cgtattgacc aatactgtat
    38701 cgataaaagg ctgattcaaa tgcttgtgta aaacacgtat atgatcggca tctgtgaaat
    38761 gttccgtttc ccctttttgg gtcatgatat tacagatata gacggtttca gcagttgtct
    38821 tgagcattgc ttcgccgatt tctggtatca ctaaatttgg aaggatactg gtgaataaac
    38881 ttccgggtcc caaaacgacc atgtcggcgt ccataattgc tgaaacgact tttcgagccg
    38941 ctttggcttc acttttatca tttgtgttgc agacatacac acggtcaatg gttttacggt
    39001 cgatcgcgat tttagattct cctactgcag tcgttccgtc tttaaagaca gcatgcaagg
    39061 tcaaaggagt ttctgaagaa ggataaacat gtccatctac atgcatcatt tttgctaata
    39121 attgaatcgc ctcataagtg cttccccgca tctcggaaac tgcggcaatg atcagattac
    39181 cgatcgtatg attggctaaa aactgatctt cctttttgaa acggtattgg aaaatatctt
    39241 catagaattg tggcatatcc gataacgcca ctaagacatt tctcaagtca ccaggaggag
    39301 ccatagcgat cgattctctg attgcaccgc tgcttccccc atcatctgca acagtcacta
    39361 ctgctgtgat atctgctcct tgatttcgca aacttttcag gataactggc agaccagtgc
    39421 caccgccgac cactacgatc tttggttttc ttatccggta ggttttcatt ttcacgaccg
    39481 gttcaccgtc tctttccttt tatccttgtc ccgatgtgtc acattgactt tatagttttt
    39541 ccctaaagca tttgccaaac gttcagtcaa tgccacagaa cggtgttgac cgcctgtaca
    39601 gccgattgca actgtcaagc tgctttttcc ttctttaatg tatccaggca atacagtttc
    39661 cagaagatcc aaaaatttat ggtagaatga ttgcgtttca tcaaaggaca tcacataatc
    39721 atagactggc tgatcttttc cggtcaatgg tcttagttcc gggatataat gagggttggg
    39781 caaaaaacgc acatccatca caatatccga atcgatcggc aacccgtatt taaaaccaaa
    39841 tgatacaaat tcgatccgaa atccttggtc tccctttgat ttgaatgact cattgatttt
    39901 ttcacgtaat tgtcgtggtg tcaatgtcgt cgtatcaatg atcacggaag cttgggcttt
    39961 caagtcgtct agtatcgccc gttctttacg tatcccttca gtgatcaaac cgtccatcgc
    40021 cattggatgt gctcgtcttg tttctttgta gcgagccact agttcttcgt ctgaagcatc
    40081 taaaaataag atagaagtgt cgatgaagtt cgtattttcg atttcgatca gcatgttttg
    40141 gatttcttca aagaaggaac gagaacgtaa atccactcct aaagccattt ttgtgacttt
    40201 gcctgattct ttgattaatt cccagaattt tggaatcaat cgaggcggca tattatcgat
    40261 acaaaaatag cccatatctt caaaactttg gatagctact gttttccctg caccgctcat
    40321 acctgttatg atgactaatt gcaaattatc tgtcataatt acgaactcct ctcacattgt
    40381 gcatagtaaa attatatcat tccgggcgta tcattaccag ttttcttttt cttttacaaa
    40441 aagatttgga ccaaatatct tttaggatat ttgccccaaa tccaattttt ttacttttcc
    40501 gatgctgtat ccaatgcctt gcttctttca gctagcgctc gttcaatcgt ccgatctgct
    40561 aaaatagatt gggcatcgat cactggataa tggttatctt cagcaaattc atgcatcaat
    40621 gacaattctg tgcaacctaa gataactttt tcacagttta ggcgctctac cgcttcttct
    40681 aagatttcgt aatatagctc ttgattcaaa taatccgatt ccttgatctc atgatagatc
    40741 agataattga ttttttcttg aagaacagta tcaggaatca ctgtttcgaa tccaagattt
    40801 ttgacttcgc gttcatagat tccagctttc atacttcctt ctgttcctag aatcgctact
    40861 ctccctgtcg tatgctgacg gacaagttca tttgctgctt ctcttggcat atgaaggata
    40921 ggaatatccg ttgccgcttg taattcttca aaaaagtagt gtgcggtgtt acaagtcaac
    40981 acaatgaagt tcggctgcaa taaattttgt ttctcaatat catccagcag aaacggcatt
    41041 ggattttctt ctgatcgatc taaaatataa gctgtacgat caggaactgt tgcatgattg
    41101 aataagacat aattcaaata ttcttgatct tttgtagctt tcgttcggtg gttgatcaag
    41161 cgaacaaaac tttctgtcgc catcgtgccc attccgccta aaatactgaa aaaattctcc
    41221 atgaactagc cttctttcac atgaaaatac tttttgaatc ttggaatata gttatggaac
    41281 atatatttca tcaacaacca gcgcttgatt gaccggtctt tttcataaaa cacagtagta
    41341 ccgtaacgat tttctttgat catttgctca gctagttttt tgtcttcatt ttcccgagca
    41401 tattctaaaa agattttttt gggcacaccc atccataatc ttgctttatc tgattgattc
    41461 gttccatagg tcgtttcaac aaagggtttg ttaaacactc gatcttctgt gacgaaacga
    41521 gccagattca agccgttcaa tgtcacaaag aaactgctac gaccttgtct caaattgatt
    41581 tcaaataatt tatattctcc atctctagga tcatacttca tatcgaaatt ggcaaagcct
    41641 gtatattcga ttttttccaa aaatgcctta atggtttgat agattttttc gttataatct
    41701 ggcatgatca caacgtaatt accaatcgat gctggtgtag gatcttctaa cagtggatga
    41761 ccaagacaca tcatccgaac ttgatgatct tcatctacat acgcatttag cacacgcata
    41821 ttactatcat cacccgggat aaaatcttgt acaatcattt cacttttata accggcttcg
    41881 tatatccggc ctaaaattaa atcgaactct tctctagtct ctaaaataaa agcttttttt
    41941 cttccttcaa attggacaga aagatactcc acactgtttg caggtttcaa agcaactggg
    42001 aattcaaaag gcaattcctg ttccaaatgt ccatttacta acatttcttc tctgacgatc
    42061 aatgttttag gatacggaag atcatattct tcacaaactt cataaaagct tactttattg
    42121 atcagtcttt caaataaaga ataatcaata tatggacaga taaaatattc tgacagctct
    42181 tgtttatgtt gggagatcaa ttctgcatat ccatcaccac aagcaatcaa aagatatttt
    42241 ttactcttat cagaatatct ttcttttgcc aaacgaagca tcgtctcgat aaacacaggg
    42301 tctttatcaa atcctggaat aacttctaca ttgacaattt tactgtagcg ggtaggggct
    42361 aactgatccg aagcatatgc ttggcagatt ttgccgtatg cttcattaaa agaacgtgcc
    42421 ataccataaa cgttcatgtc gcttcctaat aatataggga taaattcttc attttcaata
    42481 ctgttcatca ttatctctca cttactcata atctcttgtt tttcttttcc aactacacaa
    42541 ggaatatcat accataaaga aaaaagcaat caaaggaaac tcctccgatt gctcaattct
    42601 tttttgaacg gattgaactt ataggaagtt tttgtctata ccaatcagtg tagaaagaac
    42661 taccatgtaa tgattctaga acactgatat taataaaaca agcaaaaatt cattttttta
    42721 tgttttttta actatctcag ctatctatcc tctttatacc attatttttt tcaaatagtg
    42781 acctgtataa ctttctttta cgttaatgat ttcttctggt gttccagtcg caaggatcgt
    42841 tcctccacct tctccacctt ctggtcctaa atcaatgata taatctgctg ttttgattac
    42901 atctagattg tgttcaatca ctaaaactgt gttaccagca tctactaatc tttgtaatac
    42961 atgcaacaag cgggcgatgt catcgctatg aagtcctgtc gttggttcat ctagtatata
    43021 gaaattcttt ccattagaga ttttgtgcaa ttcactggca agtttcatcc gttgtgcctc
    43081 accaccggac aatgtcgttg ctggttgccc catcgtcaca taacctaagc caacatcaac
    43141 aatcgtttgc agtttgcgat gaatctttgg aatgtgcttg aagaattcta cagcatcttc
    43201 tactgtcatt tccaaaatat cagcaatgct ttttcctttg taatgcactt ctaatgtttc
    43261 agagttatat cgtttgccat gacatacttc acaaggaaca tagacatcag gcaaaaagtg
    43321 catttcgatc ttaataattc catccccgcg acaagcttca caacgaccgc cttttacgtt
    43381 gaaactaaaa cgccctttct tataaccccg catttttgcc tcgttcgttt gagcaaataa
    43441 cccacggata tcatcaaata cacttgtata agtcgctgga ttacttctcg gcgtccggcc
    43501 gattgggctt tgatcgatat cgatgatctt ttcgatactt tcgtagccgg aaattgtctt
    43561 gaatttacct ggcttagcag aattcttatt taatttttgc gctaacgatt tctttaagat
    43621 actattgacc aatgtactct ttccagaacc agacacacct gtgactgcta caaattctcc
    43681 taatggaaat tcaacactaa tatttttcag attgttttct gaagcaccag tgatcttgat
    43741 tgctttccca ttacctttac ggcgttcttt tggtacaggg attgattttt tccctgaaag
    43801 atatttccct gtcaaagaat tttcatcatt agctacttct tcaggagtgc ctgcagccac
    43861 gatttgtccg ccttgttctc cggccccagg tccgacgtca atcagataat ctgctgccca
    43921 catcgtatct tcatcatgtt cgactacaac gagggtgttg ccaagatcac gcattttctt
    43981 taatgagcca atcagtcgat cgttgtcgcg ttgatgcaat ccgatcgatg gttcatctaa
    44041 aatatacaaa actccagaaa gattcgatcc aatttgtgta gccaatctga ttcgctgcgc
    44101 ttctccccca gataatgttc cggaagcgcg actcaaagtt agatagtcta gtcccacatt
    44161 ttccaagaat gaaagtctgt catttacttc ttttaaaatc ggtttggcaa ttactttctc
    44221 ttgttcagat aacgttaagt ttgagaagaa atctactgct ttttgaatag acaactcatt
    44281 tgtttgcccg atattcgttc cattgatttg aacagataga gcttggggat tcaatcgata
    44341 acctttacat gtctgacaag ttaattctgt catataaaga cgcatttgct cacgcgtgaa
    44401 atcactgttc gtatcatgat aacgacgctc gatattcttc aatacccctt cgaaagtcgt
    44461 ttctacatcg cgaacgccac caaaatcatt ttcataatgg aaatggaaaa gatcatctgt
    44521 cccattcaag atgatatcct gatgctcttc cggtaattct tcgaatggtg tatctaaatc
    44581 gataccgaaa ctttcacaag cctgcgctaa catttgagga taatactgag agctgattgg
    44641 attccatgga acgatagcac cttgactaag tgtcttcgtt ctatctggaa tcactaaatc
    44701 ttgatcgact tctaatttga cacctaaacc atcacagtct ggacaagctc caaaaggcgc
    44761 attgaatgag aacagtcttg gttctagttc gccaacagta aatccgcaat aaggacaggc
    44821 atagtgttcg ctaaacaaca tttcttcttc accgatcacg tcaaccagag catagccgtc
    44881 tgccaatcgc agagccgcct ctaaagaatc aaatagacga gaacgaatcc cttctttcac
    44941 aatgatccgg tcgatgacga tcgcgatatc atgttttttg tttttctcca gctcaggcac
    45001 ttcacttaaa tcatagatct caccatcgac tcgaacgcgg acgtatccct ctttttggat
    45061 tcgttcgaat atttttttat gttgtccttt tttctttacc acgatcggtg ccaacacttg
    45121 gatctttgtg cgctcaggca aagccattac ttgatctgcc atttgatcta ctgattgact
    45181 ggtaatctcg atatgatcgt tgggacaaat cggatgtcct acacgtgcaa acaacaagcg
    45241 taaatagtca ttgatttccg tcacagtacc tactgtcgac cgaggatttt tacttgttgt
    45301 tttctgatca atagaaatcg ccggactcaa tccgtcgatg ctatctacgt ccggcttatc
    45361 catttgtcct aaaaactgac gagcataagc agaaaggctc tctacgtatc tccgctgccc
    45421 ttcagcgtac agcgtatcaa atgctaggga actttttcca gaaccggaca aacctgtaac
    45481 gaccacgaat ttatctcgtg gaatcgttac atctatattt ttcaagttat gggcacgtgc
    45541 accgtgaatc gtaattttat cgttagccat gttgtcctcc tactttgaag cttttaattc
    45601 taaaatcata tctcgcacat tagcggctgt ttcaaaatcc aatgctttcg ccgcattttt
    45661 catttctttt tctaatttca tcaataacgt atctttttct tctttcgtca tttcttcata
    45721 cgagacttgt accgcttctt ttgtatcatc ttccgattct ttcgtaatag aaatcaaatc
    45781 tcggatttct ttaatgatcg tttttggaac gatcccatgt tcttcattgt acttctgctg
    45841 aatcgtacgg cgtcttgatg tttcatccat cgctagacgc atagaatctg ttactttgtc
    45901 tgcgtacatg attactttac cttcttcatt ccgagcagct cgtccaatcg tttggactaa
    45961 ggaacgttca ctacgtaaaa agccttcttt atccgcatcc aagatagcaa caagggaaac
    46021 ttcaggtacg tctaatcctt cacgaagcaa attgattcca actaacacat caaattcacc
    46081 taatcgaaga tctcgaatga tttctgttcg ttccagcgtt ttgatatcac tatgcaaata
    46141 tttgactttg atccctaatt ctttgaaata atctgtgagg tcttctgcca tttttttcgt
    46201 caatgtcgtt acaaacaccc gctggtcttt ttcgactcgt tcatttattt cgcctactag
    46261 atcatcgatt tgtcccatga ttggacgaac ttcgatcact gggtcaagca gccctgtcgg
    46321 acggatgatt tgttggataa cggtatctgt ttgttcatgt tcatatggtc ctggcgtcgc
    46381 agagacataa atgatctgat tgacatgttt ttcaaactct tctaaccgaa gtggccgatt
    46441 gtctaaagca gatggcaaac ggaaaccgta atctactagc atctgttttc tcgctcggtc
    46501 accattatac attcctctga cttgaggcat ggttacgtgc gattcgtccg ccacgatcaa
    46561 aaaatcttca ggaaagaaat ccaatagcgt ataaggcggt tcaccttctt tacgtccgtc
    46621 catatgccga gaataatttt caattcccga cgtgtagccc atttctaaca tcatttcaat
    46681 atcgtaattg gttcgctgtt ctaaacgttg agcttccaga agtttgtttt catttcgcaa
    46741 gacagttaaa cgctgttcta attccgcttt gatattcgct acggcatgct ccatatgttc
    46801 atcattcgtc acaaagtgag tcgccgggaa gattgccacg tgttccgttt ctcctaaaac
    46861 ttcacctgtc agtgcattga cttctcggat acgttcgacc tcatctccaa aaaactccac
    46921 acgtaaggca cgttcatctc ttgaagccgg aaaaatctcc acgacatcac cgcgcactcg
    46981 aaaacgaccg cgctgaaaat ctatatcatt tctttcgaat tggatactga ctaagtcacg
    47041 aatcaactga ttgcgatcta gttctgctcc ctgtcgaatc gaaactactt gtttttggta
    47101 ctcaaaaggc gaacctaatc caaaaataca tgaaacggat gcaatgacga tcacatcatt
    47161 tctttctaat aatgaactag tagccgaatg tcgtaatttg tcgatctcat catttacact
    47221 tgaatctttt tcgatatacg tatcgcttga aggcacataa gcttcaggct gataataatc
    47281 ataataagaa acaaaatatt caacagcatt gttagggaaa aattccttga attccccgta
    47341 cagctgacca gccaatgttt tgttatgggc aataattagt gttggtttat tcactttctc
    47401 aataagattg gaaatcgtat atgttttccc tgttccagtc gcacctaata agatttgtgc
    47461 ttttttcccg ccgactacgc catccactaa ttggttaatc gcttcaggct gatcaccagc
    47521 cggttggtat ttcgagacaa gctcaaagtg tcgggaggta tctctctcaa tcattcttcg
    47581 tcctcctttt cattcattcc ttttcatttt acgaccaata aaaagaaaag gaaagcaaag
    47641 gcattcctta ctaaaaaatt ataccacacc gaacatactt tcgccacttt tttatcttat
    47701 tatgcatgtt acgttttata acataaaatg ttagaaactc gcattttttt cattttttcg
    47761 agcttacagt cttgtctgat tcagcacttt tcgcctataa taataaaata ttggaaattt
    47821 aggaggttga ttatgaaaag aaagcattta tttcactcat ttgttttctt aatgacagga
    47881 cttgtgacac ttgcgtttgg acatgttgca caagctgaca acactacaac ggcagagtca
    47941 agcacaagcg agacaatgca aaaaatagag cccaaaaaag acgtatacgt aattgccagt
    48001 gactctgctt ttgctccatt tgaatttcaa aacgcaaagg gcgagtacga aggcatcgat
    48061 gtaaaattga tggaaaacat cgccaaactg caaggattta acatccgaat ggactaccgt
    48121 ggtttctctg ctgctttgca agctttggaa tcaggacaag cagacggaat gattgccgga
    48181 atgactgtaa cagatgaacg aaagaaaagc tatgattttt ctgattctta ctttgatagc
    48241 gggatccaat tagctgtcaa aaaaggagat acttcgatca agtcttatga agatttgaaa
    48301 ggaaaaacag tcggagcaaa agttggtacc gaaagtgctg aatttttaag cgaccatgaa
    48361 tctgaatatg gctattctat caaatatttt gatgctgctg atcaattgta cgacgggtta
    48421 aaagttggcg caatcgatgc aatgatggat gattatccgg taatcggtta tgcgatcaaa
    48481 caaggacaag aattggaaac accgatcaaa aaagaatctg gcggaaaata tgcatttgcc
    48541 gttaaaaaag gacaaaatcc tgagcttctt aatatgttca atgaaggatt gaaagaatta
    48601 aaaagaacag gcgaatacga taagattatt aaagaatatg tagcggatgg ttcagaaact
    48661 tccagtgagt cttccactgc tgatgaatca acatttcttg gacttatcca aaacaactat
    48721 caagcattgt tgaaaggact ttggagaaca attctcctaa ctttgatctc ctttgctttg
    48781 gctatagtaa tcggtgtgat ttttggatta ttcagtgtag caccaatacg cgctcttcgc
    48841 acaattgcta gtatttatgt cgatatcatc cgaggaattc caatgatggt tcttgcgttc
    48901 tttattttct ttgggttgcc tggtatcgtt ggatttacga ttcctgactt ccttgctggt
    48961 gttatcacct tgactcttaa cgcaagtgct tatatcgcag agattgttcg aggcgggatc
    49021 aacgccgttc ctgtcggaca aatggaagct tccagaagtt taggtctttc ttataatcgg
    49081 acaatgcaaa aaatcatttt gccgcaagcg atacgcatca tgatcccatc attcgtcaat
    49141 cagttcgtta tctcattaaa agacacaacg atcatttccg caatcggtgt agtcgaattg
    49201 ctgcaaactg gtaagatcat tgttgcacgt acaacacaaa gtacgtatgt ttacttgatc
    49261 attgcgatca tgtacttgat tttgatcaca gcattgacaa aattagcaaa agttttagaa
    49321 aagaaggtga agtaagatgg cagaaaaaat cttagttgag catttagtca aaaaatacgg
    49381 cgataacaca gtattaaacg acatcaatgt gtccatcaac gaaggagatg tcgtctgtat
    49441 cattggccct tcaggttcag gaaaaagtac atttttgcgt tgcttgaatc gtttagaaga
    49501 accttctgac ggtgaaatta ttattgacgg cgcccacttg atggataaaa gcacagatat
    49561 caacttggtg agacaacata tcggaatggt attccagcac tttaacctat tccctcatct
    49621 ttctattttg gaaaacatca ttttagcacc gatggacctg aagaaagaat ctagagaaga
    49681 agcagagaaa aaagcaatca aattgctgca gacagttggt ctagaagata aaaaagatat
    49741 gtatcctgaa atgctttccg gtggacaaaa gcagcgtgtg gctattgctc gtgctctagc
    49801 gatgaaccct gacatcatgc tttttgatga gcctacatct gcactcgacc ccgaaatggt
    49861 cggtgatgtc ttgaacgtca tgaaaaagtt agcagaacaa ggtatgacga tggttatcgt
    49921 tacgcacgaa atgggcttcg ctaaagaagt ggccaatcgt gtcatgttca ttgacgacgg
    49981 aaacttttta gaagacggaa cgcctcaaca aatctttgaa aatccgcaaa atgaacggac
    50041 aaaagatttc ttgaataaag tattaaatat ttaataaaaa ccaaaaatgc ccggtcgctt
    50101 catgacgacc gggcattttt agtttcttgt tttttcactt aactgtacca gccgcatcgc
    50161 ttgttctcct gcacaaacaa gcaatcgctc tacctgttcc atcgcataag aaagtaccat
    50221 cggttcattg acgatatcaa gtacatagtc gataccacct tcagaaacta tgatgattat
    50281 cttattctaa agctggcttt tttgttggta caaacatgac aactacaaaa aaaccaggcg
    50341 aacgtatgat tgttcgtctg gtttttaatt attaatgact tagcgttttt actagaaata
    50401 gaaaatttgg gcaagtctac tttatatttt tactagaaaa actacttccg ctttttcccg
    50461 ccaaaagcat ctttaaactt atcgaagaaa ccgtcttctt gctgttcttc aactttgatg
    50521 ccgccagctt cagcgaatgc acgtaatgct tctctttgtt gttcatttag atttttcggt
    50581 gtgatcagtt ttactttgac atgttggtcg cctgttgcac caccacgtaa cttcggagcg
    50641 ccttttccac gtaaacggaa gttcgtacct gtctgtgttc cagcagggat cttcaatttc
    50701 acatctccat ggacagttgg tacttggact tcatccccca aagctgcttg tacaaaactt
    50761 aaaggcaatt catagtaaat ttcagaacct tcacgatcaa agatatcact ttcttctaca
    50821 cgaaaaacca catacaaatc accaaatggt ccgccgtttt ctccagcttc tccttgacca
    50881 gctaaacgca tttgttgtcc ttcttctaca ccagctggta cattcacttt aactgaatgt
    50941 gctttcttct catgtcctga gccatgacag gttggacaag gttctttgat ttctttccct
    51001 gtgccatgac aaacatcaca agtttgacgg ctcatcactc gtccaagtgg cgtttggcgt
    51061 tctacattga tcgtacctgc accatgacat ttatgacaag tagttggttg tgtgcctggt
    51121 tttgcaccgt ttccaccaca agtatgacag atttcttccc gattatactt gatttctttt
    51181 tctacaccaa aaatcgcttc ttcaaaagac aagtcgaccg tatattgcaa atcagcacct
    51241 tgacgaggcg catttggatc gactgaccga ccgcctcctc caaagaaaga gtcgaagata
    51301 tcttcaaaac caccgaagcc gccaccagag aagccgccga atccaccaaa tccgccacca
    51361 gctccaccag caccgtaatt tggatctgtg cctgcatgcc cgtattggtc gtatgcagcg
    51421 cgtttttgcg ggtcacttaa aatctcatat gcttctgata cttctttgaa tttttcttcc
    51481 gcatcaggtt ctttattgat gtcaggatgg tattttttcg aaagcttacg ataagctttc
    51541 tttatttcat cttctgatgc tccttttgag agccctaaga cttcataata atcacgtttt
    51601 gttgccatag gctttcctcc tattggttct catcattttc acgtcgcttg ttatcatatc
    51661 atattttgtt tgaaacaaaa accgatactt caaactattt ttcaaaagaa aaagccaaag
    51721 tagcaacttt ggccttctct cgaaattcag tgaactgatt atttatcgtc gccatcgact
    51781 tcttcaaaat cagcatcaac gacatcatct gctccgcctt gagctgcttc tggattttct
    51841 tgagcttgtt gttgtgcagc ttgttcgtat agtttaacag tcaagttttg aacgatttcg
    51901 ttcaatgcat cgcgtttagt tttcatttcc tcgatgttgt ttgcttcaac agcggctttt
    51961 aattcgtcac gagctgtttc tgcttttttc acttcttctt cgtccacttt gccttctaat
    52021 tctttcaatg ttttgtccac agagaatagc aatgcgtcta catcattacg taggtcaact
    52081 tcctctttac gagctttatc tgcttcagcg tttgcttctg catctttcac catacgttcg
    52141 atttcttcgt ctgttagacc tgaagaagat ttgatcgtga ttttttgttc tttttgtgta
    52201 cctagatctt tcgcagatac atttacaatc ccgtttttat caatatcgaa tgttacttcg
    52261 atttgtggaa taccacgagg agctgctgga atatctgtca attggaatct tcctagagtt
    52321 ttgttgtcag cagccattgg gcgttcacct tgtaatacat ggatgtctac tgctggttga
    52381 ttatctgccg cagttgagaa tacttgtgat ttacttgttg gaatcgttgt attgcggtca
    52441 atcaatttag tgaacactcc acccattgtt tcgataccta atgacaatgg tgtaacgtca
    52501 agtaaaacaa catctttcac atcaccagtg atcacgccac cttggatagc agcacccata
    52561 gcaactactt catcagggtt aactgatttg tttggttctt ttcctgtttc tttacgtact
    52621 gcttctacaa ctgcagggat acgagtagat ccaccgacta aaataacttc gtcgatttct
    52681 gattgtgaca gacctgcatc ttttaacgct tgacgtactg gtacttttgt acgttctact
    52741 aagtcagctg ttaattcatc aaattttgca cgtgtcaatg tcatttccaa atgcaatggt
    52801 ccagattctc ctgctgtgat aaatggcaag ctgatttgtg tgcttgatac gccagataaa
    52861 tcttttttcg ctttttcagc agcgtctttc aagcgttgta aagccatttt gtcttgagac
    52921 aagtcaatgc tgttttcttt tttgaattct gctaccatgt agtcgatgat tttgttatca
    52981 aagtcgtccc cacctagatg gttgtcgcca gctgttgaca atacatcaaa gacaccgtct
    53041 cctagttcaa ggatagatac gtcaaaagta ccgccaccaa ggtcgaatac taagattttt
    53101 tcgtcgcgat ctgttttatc taaaccatag gcaagagcag ctgcagttgg ttcattgacg
    53161 atacgttcta cttctagacc agcgatctta ccagcatctt ttgttgcttg acgttgtgca
    53221 tcattaaagt aagctggcac agtgataact gctttttcga ctttttcacc taaatagtct
    53281 tctgcgaatc ctttgatata ttgaaggatc attgctgaga tttcttgtgg tgtatatgat
    53341 ttaccttcta catctacttt gtaacctgct tcaccgatat ggcgtttgat tgatgaaatc
    53401 gtatttgggt ttgtcacagc ttgacgtttt gcaacttccc ctacttggat ttctccattt
    53461 ttgaatgata caacagaagg tgttgtacgg tttccttctg ggttagcgat gatttttgct
    53521 tcgtttcctt ccaatactgc tactgcagag ttagtcgtac ctaagtcaat accgataatt
    53581 ttactcataa ttaacgtctc cttctttaat tgaaagtgta agttttatat ccgcacaatt
    53641 attgtgcaac aatgaccata gttggtctta acacgcgatc atgtaatttg tagccttctt
    53701 gtagtacttc tacaatcgta tcggctggtg tatcttcagt cgctggaaca gtttgtactg
    53761 cttgatgaag attgggatca aaggcttctc ctttagcagg gattttttca attccttctt
    53821 cttccagtgc gttacgcaaa ctttccaaga ccatttccac gccttttttc aaacctgccc
    53881 cttgatcatc tgacacttct gtggctaatg cacgttccag gttgtcaatt gatggaagta
    53941 attttttggc taaatcttgt gaacggtatt tttgtaattg ctcacgctca ttttttcctc
    54001 gatttgccat atttgcgatt tcagcacgag cccgcaagta tttatcttcc atttcttcta
    54061 actcagcttt taatttcgct gtttcgaatt cttccgcttc agcttctgaa atacccgaat
    54121 cttctgcagc ttcgacatcg atttccggtt ctggctgagc atctgtcatt tctttatcta
    54181 gttcttcttg attttctttc acgatacagt cttcctttca ttattcagtc agctgtttga
    54241 ttgctcttac taagaaagag agaacgatca gctgcaaata gcttcgttat tcacattacg
    54301 agtttgacaa gtcaagcgag cggtagtaat cagcaagttt agctgctagt tctcttcgat
    54361 aaacatcgac tagaccgaac atccgagaat aaggcatact agtaggccct aacaaggcta
    54421 ttgtacctct tccatgacca tctatctcgt aacttgcttg aatcatactc atattctgca
    54481 ataaatcatt gcctaattca gcaccaatct tgatatgaat attgctatcc atcggagtga
    54541 tcatttgtgt aagtccgtct gagtctttca taaaagaaaa catcgatttg aactgatcaa
    54601 gatcttgacg gggctcataa tccaagagat tcatttgtcc actgacataa accttttctt
    54661 caaaaacatg accaagaacg gtgtcaaata actgagacat cccttctgtc gtctggaaat
    54721 atttatgaag aatcatcgga atctccgtac gaagtttatg gtacaccgtc atcagaggat
    54781 ctccgactag acggtcgttt acaatctgta ccattttttc tagttcctga cttcccatat
    54841 attcgggaag agtgaacact tggctttcta cattccccat atctgttacg acgatagcaa
    54901 taatctgtcg attattcaag ggtacgatac gaaagccagt cagtcggcgg tcttttattt
    54961 caggtcccaa tgaaaaagtc gtatagcttg tcagctcaga taatatctca gcagattgct
    55021 taataatctc attgatttca tgaaattctc tgcctagaga ctgacgaatc agcagcaaat
    55081 catcttctgc tacgcgagca ggttttaaca aatgatccac gtaataacga tagcctaaag
    55141 cagatggaat ccgcccagaa gaagagtgag tcttcaacag caatccttca tcttctaatg
    55201 ctttcatatc attgcgaatc gttgctgagc tggccttgac tccctcagac attaatgttt
    55261 tggaaccaac gggcacgcca ctactcgtat agtgttggat gatcagacgt aaaatgtcgt
    55321 tttgtcgctt tgttaacatc ttcctcacct ctcattagca ctcatatatt ctaagtgcta
    55381 attcacttat aaatatagca agaagccctt ctcctgtcaa gagaaagact ccatttttta
    55441 gcactctaaa cacaagagtg ctaattctaa ataattgatg taaatccagt tatatcaata
    55501 cttcaatacc tcattgtcaa actttctaaa ctttttcttt tcaagtttcg ataaaaaaca
    55561 aatattcttc aaactatttt tcaaataaaa agcgctcaaa cacattgttc cccaaaaaag
    55621 tccctcttgg tgtcaaataa acacggtctt cttcattaac aagtaaccct tcttgctcta
    55681 attcccttag cacatcaccg tacaccgatt caattgtttt ttgatagcga tcatagaaat
    55741 gttttttagt aatccctgtt ttttttctta gtcctaagaa catttcttct tctatttgat
    55801 tttttcgtgt caatttttct tcttccaata ccgggcgttc atttgctcgt aatgggcgta
    55861 aatagtgttg gattggtcct ttgttgcggt aacgaatatc tccaagataa ccgctagcac
    55921 cagctccaaa cccaaaatag tggtcattgt tccagtacat gaggttatgt tgagattcat
    55981 gtccggagag cccaaagtta cttatttcat attggtgacg tccatgcttt tccattgctt
    56041 caatcgtctc ttcaaacatt ctggtttccg tttcctgatc aggcagttcg agtcttcctt
    56101 gtcgtaccca gttcatgaac attgttttgt tttcgaggat caatgagtac aaagaatagt
    56161 gaggcagatc caaagccaaa gctttttcca aagtatcccg atttccttct agtgtttgtc
    56221 ccggtaatgc ataaatcaaa tcgatgctta cattggtaaa attttctgct tctaaaaatt
    56281 gcattgtttg atacacatct tctgcagtat gtttacgtcc aatttttttc aataagcgat
    56341 tgtcaaatgt ctgcactccc atggataaac gattgacgcc atagtttttc atcacttgaa
    56401 gtttttcacg ggtcaaatca ccaggattcg cttctacagt aaactcatta ccattttgaa
    56461 aaggcaggat ctctctagca cctgataata gtcgatcaag ttgtttagca gataaagaag
    56521 ttggtgttcc gcctcctaca taaagtgttt ccaaattcgc tgtaggatga ctttccagca
    56581 ttaatctcat ttcattcagc agcatttcca catactcatc tactggctgc ccttctaaaa
    56641 aaactttatt aaagtcacag taatagcaga tatgctcgca aaatgggata tgaatgtacg
    56701 cagaaacctt gttttctgcg ggatttattg tttctatcat tgaaattctt tctccttacg
    56761 tcgaaaagtc gtgcctgttt tcgaccttct taaaatcgcg ttcaagcacc aactcattga
    56821 aataagccgc atatttagaa tcaaggaaac ctagctctaa aaatgcttgc ttattgtcat
    56881 cggagctaca cggtgctttt acagcctctc gataaacggt aatcgcaaag ctgaccttcg
    56941 gcattaagtc aaaatctgca aaaatatgaa aagctatttt cacagattcc tccttaagtg
    57001 ctcagaccac aacgctttgc ttacaacctc tcatatacgc atactaaacg tatttggcga
    57061 acaaatccac taaaaaatcc aagaatcgac ttcttggatg atttttttac actcatgatt
    57121 cttcctaaac aattttgtcc ttattttatt cataaaaaaa tctagtcaaa tctctcactt
    57181 caagagtttt aactagattc caattattat ttttttgtaa aaataaacag tctcttaatc
    57241 atagacattt cttcggtgcc ctacttgaat agcgataaca acgaattgat tatcttctat
    57301 ctctaccaat acacgtattt gccaatcaga gctgcttctg aatttcgggc gtgaatttta
    57361 aacggcaggt cattccagcc caccaaattc attaaggatt cttccatgaa tactgttgtt
    57421 ttcccacttc ttatccaatg tttatgggca atttcagccg tttgacgatt gtattcatct
    57481 tctagttgtt ccatcgaata gttttttaga agttcagata aagtaatccc ataaaattca
    57541 gccatataag aaagccattc ttttttggcg tcacttacac gaaccgtaat catagcccta
    57601 taattgcctc tcttaaagaa tctcatgcat ttcctgatgt gattttagaa aaatttactt
    57661 gctaaaaaac tgtgaaaatg taggagctag catccttgta gtaaatgatc gagctcttta
    57721 ttatacatta aaaaagttca gaaattttga tgaagtagcc aactactttt tcatgagtaa
    57781 agcaacacta gaaaaagaat cctccagtac ttagaattta ctggaggatg tacttttgaa
    57841 gaagcctata ggttatttaa aaaatattac tacaggaagg aacgagaatt acttcgttac
    57901 ttataataga acttagtctt atatagaagg catagctcac attagcttat ttgtcctata
    57961 aaaataaatt acggtatttt agaaaagcta ttttctcttt tttcaacgat cttcagcaat
    58021 ttttctttat cgtcaggtaa ctcaagttca tgaattctag catgacaatt gggacataac
    58081 gcgatacagt tttcaatcac atcttttcca ccttgcgaca aatattctat atgatgacaa
    58141 tgtaaataag gtacaccgtc ttttataaat ggcccttttt cttggcaaag ttgacaaata
    58201 ccttttgcaa gtcgtttaac atattcagat acgaagggat tacgtttata tatttttgtt
    58261 tttgagaagc aaaactcctc ttcgttaaca tattttgcag ccattttt
//
LOCUS       DAZUMK010000004        57796 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000004 DAZUMK010000000
VERSION     DAZUMK010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.004"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            88..1605
                     /locus_tag="ACG808_000258"
     CDS             88..1605
                     /locus_tag="ACG808_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFS5045919.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            1577..2866
                     /locus_tag="ACG808_000259"
     CDS             1577..2866
                     /locus_tag="ACG808_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5045920.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            3041..3937
                     /locus_tag="ACG808_000260"
     CDS             3041..3937
                     /locus_tag="ACG808_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5045921.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            3951..4814
                     /locus_tag="ACG808_000261"
     CDS             3951..4814
                     /locus_tag="ACG808_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5045922.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            4820..5383
                     /locus_tag="ACG808_000262"
     CDS             4820..5383
                     /locus_tag="ACG808_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS5045923.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            5405..6916
                     /locus_tag="ACG808_000263"
     CDS             5405..6916
                     /locus_tag="ACG808_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFS5045924.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            6928..7887
                     /locus_tag="ACG808_000264"
     CDS             6928..7887
                     /locus_tag="ACG808_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFS5045925.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            7973..9052
                     /locus_tag="ACG808_000265"
     CDS             7973..9052
                     /locus_tag="ACG808_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5045926.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(9304..10704)
                     /locus_tag="ACG808_000266"
     CDS             complement(9304..10704)
                     /locus_tag="ACG808_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFS5045927.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            10922..12583
                     /locus_tag="ACG808_000267"
     CDS             10922..12583
                     /locus_tag="ACG808_000267"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFS5045928.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            12540..13238
                     /locus_tag="ACG808_000268"
     CDS             12540..13238
                     /locus_tag="ACG808_000268"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFS5045929.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            13254..14678
                     /gene="araA"
                     /locus_tag="ACG808_000269"
     CDS             13254..14678
                     /gene="araA"
                     /locus_tag="ACG808_000269"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFS5045930.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            14985..16145
                     /locus_tag="ACG808_000270"
     CDS             14985..16145
                     /locus_tag="ACG808_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS5045931.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(16122..16262)
                     /locus_tag="ACG808_000271"
     CDS             complement(16122..16262)
                     /locus_tag="ACG808_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFS5045932.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            16263..18122
                     /locus_tag="ACG808_000272"
     CDS             16263..18122
                     /locus_tag="ACG808_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS5045933.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            18232..18666
                     /locus_tag="ACG808_000273"
     CDS             18232..18666
                     /locus_tag="ACG808_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045934.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(18925..19086)
                     /locus_tag="ACG808_000274"
     CDS             complement(18925..19086)
                     /locus_tag="ACG808_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045935.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(19105..20127)
                     /locus_tag="ACG808_000275"
     CDS             complement(19105..20127)
                     /locus_tag="ACG808_000275"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFS5045936.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            20255..20608
                     /locus_tag="ACG808_000276"
     CDS             20255..20608
                     /locus_tag="ACG808_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFS5045937.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20620..21786
                     /locus_tag="ACG808_000277"
     CDS             20620..21786
                     /locus_tag="ACG808_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS5045938.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(21878..22498)
                     /locus_tag="ACG808_000278"
     CDS             complement(21878..22498)
                     /locus_tag="ACG808_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFS5045939.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(22690..22986)
                     /locus_tag="ACG808_000279"
     CDS             complement(22690..22986)
                     /locus_tag="ACG808_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFS5045940.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            23190..23840
                     /locus_tag="ACG808_000280"
     CDS             23190..23840
                     /locus_tag="ACG808_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFS5045941.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            23856..25583
                     /locus_tag="ACG808_000281"
     CDS             23856..25583
                     /locus_tag="ACG808_000281"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5045942.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            25580..27472
                     /locus_tag="ACG808_000282"
     CDS             25580..27472
                     /locus_tag="ACG808_000282"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5045943.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            27830..28036
                     /locus_tag="ACG808_000283"
     CDS             27830..28036
                     /locus_tag="ACG808_000283"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045944.1"
                     /translation="MQKCRKPFSQNFSYYSKLSDFFTTSSLNGVQKLASAVISPTKRK
                     IGGAIFVWLFLLLDAEQLFHNLFL"
     gene            28176..29045
                     /locus_tag="ACG808_000284"
     CDS             28176..29045
                     /locus_tag="ACG808_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5045945.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            29254..30279
                     /locus_tag="ACG808_000285"
     CDS             29254..30279
                     /locus_tag="ACG808_000285"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFS5045946.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHGG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            30318..31268
                     /gene="manA"
                     /locus_tag="ACG808_000286"
     CDS             30318..31268
                     /gene="manA"
                     /locus_tag="ACG808_000286"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFS5045947.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            31362..33065
                     /locus_tag="ACG808_000287"
     CDS             31362..33065
                     /locus_tag="ACG808_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5045948.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            33067..35100
                     /locus_tag="ACG808_000288"
     CDS             33067..35100
                     /locus_tag="ACG808_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFS5045949.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            35111..35551
                     /locus_tag="ACG808_000289"
     CDS             35111..35551
                     /locus_tag="ACG808_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5045950.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(35751..36878)
                     /locus_tag="ACG808_000290"
     CDS             complement(35751..36878)
                     /locus_tag="ACG808_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5045951.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(36881..37558)
                     /locus_tag="ACG808_000291"
     CDS             complement(36881..37558)
                     /locus_tag="ACG808_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS5045952.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            37822..39588
                     /locus_tag="ACG808_000292"
     CDS             37822..39588
                     /locus_tag="ACG808_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFS5045953.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            39603..40040
                     /locus_tag="ACG808_000293"
     CDS             39603..40040
                     /locus_tag="ACG808_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5045954.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            40053..41204
                     /locus_tag="ACG808_000294"
     CDS             40053..41204
                     /locus_tag="ACG808_000294"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFS5045955.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(41266..41964)
                     /locus_tag="ACG808_000295"
     CDS             complement(41266..41964)
                     /locus_tag="ACG808_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFS5045956.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(41957..43309)
                     /locus_tag="ACG808_000296"
     CDS             complement(41957..43309)
                     /locus_tag="ACG808_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFS5045957.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(43532..44932)
                     /locus_tag="ACG808_000297"
     CDS             complement(43532..44932)
                     /locus_tag="ACG808_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFS5045958.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            45093..45830
                     /locus_tag="ACG808_000298"
     CDS             45093..45830
                     /locus_tag="ACG808_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFS5045959.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            45955..46434
                     /locus_tag="ACG808_000299"
     CDS             45955..46434
                     /locus_tag="ACG808_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFS5045960.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            46431..47198
                     /locus_tag="ACG808_000300"
     CDS             46431..47198
                     /locus_tag="ACG808_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFS5045961.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            47428..47997
                     /gene="lepB"
                     /locus_tag="ACG808_000301"
     CDS             47428..47997
                     /gene="lepB"
                     /locus_tag="ACG808_000301"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFS5045962.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(48101..48703)
                     /locus_tag="ACG808_000302"
     CDS             complement(48101..48703)
                     /locus_tag="ACG808_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFS5045963.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            48809..49039
                     /locus_tag="ACG808_000303"
     CDS             48809..49039
                     /locus_tag="ACG808_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045964.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            49030..49278
                     /locus_tag="ACG808_000304"
     CDS             49030..49278
                     /locus_tag="ACG808_000304"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5045965.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            49384..49968
                     /locus_tag="ACG808_000305"
     CDS             49384..49968
                     /locus_tag="ACG808_000305"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFS5045966.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            49971..51044
                     /gene="prfA"
                     /locus_tag="ACG808_000306"
     CDS             49971..51044
                     /gene="prfA"
                     /locus_tag="ACG808_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFS5045967.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            51037..51876
                     /gene="prmC"
                     /locus_tag="ACG808_000307"
     CDS             51037..51876
                     /gene="prmC"
                     /locus_tag="ACG808_000307"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFS5045968.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            51869..52048
                     /locus_tag="ACG808_000308"
     CDS             51869..52048
                     /locus_tag="ACG808_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045969.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            52070..53095
                     /locus_tag="ACG808_000309"
     CDS             52070..53095
                     /locus_tag="ACG808_000309"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFS5045970.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            53270..54514
                     /gene="glyA"
                     /locus_tag="ACG808_000310"
     CDS             53270..54514
                     /gene="glyA"
                     /locus_tag="ACG808_000310"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFS5045971.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            54667..55614
                     /locus_tag="ACG808_000311"
     CDS             54667..55614
                     /locus_tag="ACG808_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFS5045972.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            55748..56377
                     /gene="upp"
                     /locus_tag="ACG808_000312"
     CDS             55748..56377
                     /gene="upp"
                     /locus_tag="ACG808_000312"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFS5045973.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(56516..56758)
                     /locus_tag="ACG808_000313"
     CDS             complement(56516..56758)
                     /locus_tag="ACG808_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5045974.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            56879..56951
                     /locus_tag="ACG808_000314"
     tRNA            56879..56951
                     /locus_tag="ACG808_000314"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:56912..56914,aa:Thr,seq:cgt)
     gene            complement(57070..57708)
                     /locus_tag="ACG808_000315"
     CDS             complement(57070..57708)
                     /locus_tag="ACG808_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFS5045975.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT        19207 a        10110 c        11956 g        16523 t
ORIGIN      
        1 taaaaaataa gtaagtttaa catttatatt attgacaacg ctttcaacga ggtgtattat
       61 tcatgtataa attaaaggag gtcagttatg gaagccacaa tgttgattag tagtgtcaag
      121 cgtgtaggca aagttgatcc gcggttgtac agttcattta ttgaacatat gggacgagca
      181 gtatatgaag ggatttatca gccagatcat ccatcagcta ataaaagtgg atttcgccaa
      241 gacgtcattc acttggtaaa agaattgaac attcccatga ttagatatcc tggtggaaat
      301 tttgtgtcag gatttcgttg ggaagacagt attgggccca tagcaaatag acctagaagg
      361 ttggatttag cttggcggac aattgaaacg aatgaagttg ggattcatga attttataac
      421 tggtgtcagc atgtgggggc cgaaatcaat atggctgtca atctgggtac tcgtggagta
      481 gatgcagctc gtaatttagt cgaatattgt aattttccta aaggaactta ttggagtgat
      541 ttaaggcgtt cgaatggtca agagagccct tttggaatta agacatggtg tttagggaat
      601 gaaatggacg gtccttggca gattggacat aaaacagcgg aagaatatgg acggttagca
      661 gaggaaacgg ctaaggcgat gaaactcgta gatgattcaa ttgaggtggt tttatgcgga
      721 agttcgaatc ggcaaatgcc tacatttggt gattgggagt taacagttct tgatcaagcg
      781 tacgatcaaa tagattattt gtctctgcat caatattatg gcaaccaaaa aaatgatctt
      841 tctcattatc ttgcacgttc cttagacatg gatcggttca tttctgaagt gacagcgatg
      901 tgcgatacag tcaaggcgaa gaaacatagt aagaagcaga tcaacctgtc ctttgatgaa
      961 tggaatattt ggtatcattc agcggaacaa gatgagcaag cagcgccttg gcagattgct
     1021 cctcctttac tagaagataa ctataatttt gaagatgcat tactattagg ctgtctatta
     1081 atcactttat tgaaacatgc agatagagta aagattgctt gtcttgctca attggtaaat
     1141 gttattgcac caatcaggac agaaacaaac ggagaagctt ggagacagac gatctattat
     1201 ccatttatgc aagctgctgt gtatggacaa ggagaggtgt taagcccaca aattcgttca
     1261 gcaaactatg cgacggaaga ttttgaagaa gtgccgtatt tagaaagtat agccgtaata
     1321 aatgacaaag aacttgtcat atttgcagtt aaccgtggag aagaagaaat gaatctaact
     1381 gttcacttgt ctgaattatc attagaggga gtgattgatt tttctgaaat gagtggattt
     1441 gatacaaaag caactaatct tcctaaaagt gaacaagtaa aaccaagtca ttcaacaaat
     1501 gtaaaagctg aagatgggaa aatcaattgt ttattgaaac cgctatcttg gaatgtggtc
     1561 agatgtcgtt tatcagatga aggaggaaca aaaaatgaaa ggtaaaaaat ggttaattgg
     1621 ctctttgttg gtagctgcaa caggtatttt aggagcttgc ggtggcggaa gttcagcaga
     1681 atctgattct gataaagaag tgactttgac tttttggaat gggtttactg catctgatgg
     1741 tgaaatattg caagaaatcg ttaatgaatt taataaaaca aatgataaaa acatcaaaat
     1801 tgaaatggat atcatgactt gggccaattt gaatgaaaaa cttcctacag cgatttcttc
     1861 taaaaaagca cctgacttta ttgcattaaa ttatccagat tttgcacagt atgtagaaaa
     1921 cggagcggta caaccattag acgatttctg ggaatatgat ggagtagata aatctgattt
     1981 taaagaaacg gctattgaat taggtgaagt agaaggtaaa cagtattttg tccccatgca
     2041 ggtacaaggc atgtatatgt attggaataa tgatttgttt aaacaagctg gattagatac
     2101 tgaacaacca cctactactt gggaagaatt gacagaaatg gcaccaaaat taactgatag
     2161 tagtaaaaat gtatcaggtt ttgtcttcaa taaggatgga actgcaccgt tatataactg
     2221 gatgttagca aatggcggta aattagtgaa cgatgattat actaagtctg aatttgcgtc
     2281 tcctgagaac cttaaaacgt taaaagcgat ccaaaaaatg atccatgtag acaagactgg
     2341 tccagaatcg atttctggcg ccgaaatgga caatttaatg aatgctggtc aactagccat
     2401 tgaaatcaat gggccatggt taaataacgg attgaaagca aacgagattg attataatgt
     2461 gactacctta ccacaaggga gtgattcaga caaaaaaaca gctattttag atggcgtagg
     2521 atatgccatt ccggcaagta cagataataa taaaaaagaa gctgtctatg agtttttgaa
     2581 atattggaat acaactgaaa ttggtaaaaa atggagtatc gaaaatggtt ttcctgccta
     2641 cttggattca gtagcaaaag atgaagaagt aaaaaatgat cctattgtat ctgaattatc
     2701 gaaacaaatg gactatgcag agccatattt accaggtttt acccaaatct ctgcgatcaa
     2761 taacgatatc atcaatccac tgattgaaaa attattagca ggagaagatc ctgagacact
     2821 catgaacgat gcagatgatg ccatcaatca attactgcaa aaatagtaca gaactttata
     2881 caggatagac tgttggcaag atacagatat ctgaatgatc gacctagtat ttgcagatag
     2941 gtgttattgc aaaaatcaca gcgattgttc ggatatctta ttcagattcg tttaaaccaa
     3001 gattgtatat tttatcaaaa tgaaagatga ggtggactat atgaacggat tcaccaaata
     3061 ttggaatcgt cctgatcgtg ctgcgtattt gtttttaact ccatcttttt tagttttatt
     3121 attatttact gtcataccgc ttttaggaac atttgctatt agttttacag atttgaatat
     3181 tttctttaca tcaagaaatt ttgtaagttt tgaaaatttc acacgtatat ttagtgatga
     3241 acgagcagtt aattcgttga ttcattcttt atatttcacc gtactcgaaa cgccaacaca
     3301 aattatcgtt ggtttattag cagcagtagc attatctaaa aataccagat tcaatcgttt
     3361 ttgtcgttcg acattttaca ttccagttat ttgttcattg acgtcaattg cgattatttt
     3421 ttctatgttg cttgatccga atgtaggggc gattccttac ttgttttcac aagtcggctt
     3481 gccgatccct caattttttc gcgatccaac ccttgctatg ccgacggtag ccatcatgac
     3541 tgtttggaag aattttggtg ttactatgac gattctcttg actgctattc agggaatctc
     3601 tccctctttg tatgaatctg ctcaaatgga tggtgcaaca aatcgacagc agttttttaa
     3661 cattacgcta cctcagatcg ttccctcctt aggtttttgt attttaacga atctgattgg
     3721 ttcgatgcaa gtgtttgacc aagtatatgt tgcaaccgct ggtggcccac aatttaagac
     3781 agaaacagct gttcaatata tttacagtcg aggatttaca gcaccttatg aattgggcta
     3841 tgcatcagca ctgtcttcgg ttttgtttat cattgttgca actatagcta tctccactaa
     3901 tgtgtacatg tcacggaaag aaaaacaaat gaaataggag ggaaacctgt atggcaccag
     3961 aaagctcgat tacagttact gtgaatagtc gaaaagcagt tagtggtaaa aaaatcattc
     4021 gaacgattag tatgctagtt atattgttgg ttattgcgtt tccttttttg tggctgatta
     4081 tttcttcttt taaacacgaa aaagacatta tctcatttcc gcctagaata tttgcagata
     4141 gctataccct agataactat attaaggttt ggacaacgat ccctttatta gattacatca
     4201 aaaatacagt gatttttgct ggagggacag tgatcacttc tgtattcttt gattctcttg
     4261 cggggtatgc atttgctcgt atgcgtttca aaggaaaatc agttttgttc tatttcgttt
     4321 tgctgacaat gatgatccca ttccaagtct tcatgattcc tctatttatt gaagtcaatt
     4381 tgcttggcat gcttgatact tatgcaggtt tgatcattcc tcgtatgaca actgcatttg
     4441 gtatcttcat gatgcgttcc ttttttatta ctttaccaga ttctttggaa gaggcagcac
     4501 gcattgatgg attaagtgag tttaatattt ttttgaaaat catgttgcca ttgagcaaac
     4561 ctactctttt atcattaggg attttcacgc tgatgaatag ttggaatgac ttgctttatc
     4621 cattgatatt gacaagtagt tcaaaaatga gaactttacc agcagggtta gctttattta
     4681 cagggcagaa catttctttt tatggacctg taatggctgg aacggttatt tcaatgctgc
     4741 cattactagt ggtttatatt tttgcacaga aatattttgt tcaaggaaca gctatgtctg
     4801 gaatgaaaga gtgaggaaga tgaaaatagt tgagactaaa ctttatggac agttgattgt
     4861 ggtttctgat tacatcttat taggtattct ctgggtaatc gtgtgtttgc ctgttattac
     4921 tatcgtgcct gctacatgtg ctgtttttta tgtaatggcg caatggaaag gtgagggatc
     4981 aggaaatatc atttattttt tctttcaagg aataaagcaa cattttggta agaacttgtt
     5041 ccttagtctg ttaacgatca ttatttattt tcttgttaat caattattag aagaacagac
     5101 aatcgtactt tcaattagtg gatatgtagt tttacttatt tattccatgt ttcttttatc
     5161 ctggatatat gtgatttctg aaaacaaaga tatcacattt ttacaaattt ttgaacaaag
     5221 tgcatggaaa gtattttttg attttttgag aaatattctt tgttgcgtat tgttattagg
     5281 atttgtactg ctcatatttc ttttccctcc ttttattttt atctttgctg gtggcgtatg
     5341 gaaacttgta tctattatct tgactatagg aaatgaaagg tagggaaagc tgttggacga
     5401 agaagtgaaa gtaaaaatca gagaggattt tacggtagga gagatcagtt ctcggctgtt
     5461 tggctcattt gttgagcata tgggaagtgt cgtctataca gggatttatg aaccgggcca
     5521 tgaaacagct gatggtgacg ggtttagaca agatgtcctc tcttatgtaa aggaattaga
     5581 attaggtgtc attcgctatc ctggaggaaa ctttacatct ggttatgatt gggtggatac
     5641 tgtaggtagg aaagaacaac gaccaaaaaa aattgattta gcatggcgag gtatagaacc
     5701 aaattctttt ggactccacg aattttttaa atggatcagt catgtacaag cgaaaccgat
     5761 attcacggtg aatttaggaa caaaaggaat tgatgaagca aaaaatttag tagaatattg
     5821 taattttcca aaaggaacct tctggagtga tttaagaata aaaaatgggc aaaaaaagcc
     5881 ttttggcgtt aaattatggt gtttagggaa tgagctagac ggtccatggc aagtgggggc
     5941 taagagtgct tatgaatatg gtaggttagc aaatgaagcc tcaaaagtca tgaagttagt
     6001 ggatgattca attgaaacaa tcttagttgg aagctctacg ccacgtttac catcgtaccc
     6061 tgaatgggat cgtatcgcat tagaagaagc ttatgagaat gtagattatt tagctttgca
     6121 taactatatt gataagtatg atgaagaaga cttgactaaa cctcctaaaa atgagcgtga
     6181 tgatacagcg acataccttg caaaagctta tcgattcgat cgtcaaattg aagaaatcgt
     6241 tgctacttgt gattatgtaa aaggatattt gagaagtgag aaaacaatgt atctagcgtt
     6301 tgatgaatgg aatgtacatg cccaaccgga aaaaagacat agggattttg aagtcggtag
     6361 tcccatcgat tggtgttact ttactatgga agatacatta ttatttggtt cattagggct
     6421 ggctatttta cgacacgcag atcgcgtaaa gattagttgt caatcattgt tagtaaacac
     6481 aataccttta atactgacag atgctaaagg acaagcatgg aggaatccaa cttattatgt
     6541 gatgcaacac ttatcgaaat atgctaaagg tcaagtattg aaacaacaaa tcatttgccc
     6601 tgtttatgat tgtgaaaaga taaaaaatgt tccttatgtg gacagtgtga tcgtagataa
     6661 cggcaatcag ctgactattt tcttgatcaa tcgcacaaat gaacaacaaa agataacgat
     6721 tgaacatgat ttcaatttgc tagatcaagg agagcactac acattaacca gtagcgatct
     6781 gttggacaaa aatacacgtg aagaaccaga aaaaataaaa cctgaaataa actatttttc
     6841 aatagaaaaa catgacgaat taaatgtagg gatagaaaaa tactcttgga atgtaattag
     6901 aataaagaaa atgtgaggaa atttgttatg atgaagacac tttataataa tcctatcgtt
     6961 cggcaacggg cagatccttg ggtatataaa catacagatg gttattatta ttttacagga
     7021 tcggttccag gctaccaagt gattgaatta agaagggcaa agtcactgaa tgaattagaa
     7081 catgctgcaa gcctgattgt atggcaggct catgaagaag gaccgatgag tgaattgatc
     7141 tgggcacctg aagtccatta tatcaatgga aaatggtata tttactttgc tgcaagcaat
     7201 cataaaacta tacgtgatga atctcatcat caccgcatgt tcgtacttga aaatcagcat
     7261 atagatccaa tgaatacaga ttggatagaa aagggacaaa taaaaacgaa aagggaaact
     7321 ttcagtttag acggaacaac tttctcttct caaggaaagc tatattatgt gtgggcccaa
     7381 caagatcctg aaattactgg gaattctaat ctttatctat ccgaaatgga taatccttgg
     7441 acgttaaagg ggaagcagat cctcctttct atacctgagt atgaatggga aaaacggggt
     7501 ttttcagtaa atgaaggtcc agcagtcctt actcgcaatg ggaaaatatt cattacgtat
     7561 tctggtagtg caacagatga aaactatgcc atgggcctgc tgtgggctga tgagcacaag
     7621 aatcttttat taaaagaatc atggcataaa ttatcggagc cagtattcgt ttcttctgaa
     7681 aaaaataagc aatttggtcc tggtcacaac tcgtttacgg tttcagaaga cgggtgcttt
     7741 gatattttga tctatcatgc tcgaccagag aagaaccaaa aaggggatcc gttagacaat
     7801 cctaatcggc acgcgaacgc gcaatgcttt acgtggaatg agcaaggatt tccagaattt
     7861 ggtgaacctg ttccgtatgc cttatgagca caagaaacct tgatttttgc ttgaataaac
     7921 cttgcagact cgttatgata tatgtacgta caaataaaaa ggggcaaggc aaatggcaaa
     7981 atatcaagat attgcagaag agataaaaca aaagatcctt cgtgaggaat acgaagtaaa
     8041 tacaaccatt ccctcagaat tcaagctgca agaaatatat gacgtcagca gacatacgat
     8101 tcggcaagca atcgctgtgt tggtaaatga gggctatctg cgtaaagaaa aagggtcggg
     8161 cacatatgta gatgacagct ataagaaaga aaaaacacca ttgaacagaa caaaaacgat
     8221 cggtgtcatc accacatatc tctctgatta tatttttccg tcgattatcc gaggaatcga
     8281 aaatgaatta cgagaatctg ggtattcatt gcttttagcc agtacgaata acgatcacga
     8341 attggagaaa gagtgtttga agaaaatgat tgcttatggg gtagatggtt tgatcgttga
     8401 gcctacgaaa agtaatcagt ataacccaaa cttagcactt tatgttgctt tgagagaaca
     8461 aggcattccg atggtgatga tcaatgctgc ttatgaagag ttaaatgcct cttatatttg
     8521 tttagatgat gtgaaagtcg gttttatggc agcagaatat ttgataaaaa aaggacatca
     8581 gcgtctgttg ttcatcacaa aaatcgacga tcttcaagga aaatacagga tgaaaggttt
     8641 tatcagcgcg tgtgaacagt ataatgtgcg ttttttgacg gaagatatca ttactttcac
     8701 cacggaaaca cgagaaacaa tcgctgaaaa aacagtacaa cggttgaaag aagaacaagc
     8761 tttagatggc atcatttgct ataacgatca aatagctagt ttaatgctag ataagttgac
     8821 agaaaatggc tatcatattc cagatgacct atcgatcgta ggaacagacg attcttcttt
     8881 aagccattta gggaataaaa aattgacaac attgacgcat ccaaaagaaa agttgggaca
     8941 agatgccgca cggtggatcg tcacaacgat tgaaaaagga caggcacagc caaatattct
     9001 ctatgaacct gtactagttg aacgggaaac agtgaaagaa aacgaaaaat aagagacggg
     9061 tttttaccgc ctcttattgc tcctacggtt actcgtcgca atgtttgtga gagcttttgt
     9121 catcgctcaa ggctgaacgt ttctttcacc acctctccac taaacggtgt tctcctacca
     9181 actggtcaca agtcacaata ttcatcaaac atgagaagct gttttttgaa attgttcctt
     9241 gtgactggaa actaagcggt tccttcttat ttcttgaagc tgaccacttc tgtcacaacc
     9301 tctttaagaa tgatggatag aaatacgttt aggataagtt tttttagatg cactttgttc
     9361 gatttcctct aaagatttcc cacgagtttc tggtacgatc tcttgaataa ataaaacccc
     9421 taaaaagcag atgatcccaa aaatgccgaa aacgatttct tgagacatag aagcagtcat
     9481 cacaggaaaa agcaagccaa ctaggaaaga accaatccaa ttaaaagaag aggctaggcc
     9541 agatgctcgc ccgcggacag ctagtgggaa tatttcgcct acgattaccc atgtcagtgg
     9601 agcccatgtg caagaataaa atgcgacata aatacataaa aacaagagaa tcaacaacga
     9661 atttgtatga gcatccagta cagttcctaa aacagctggt aggatgaaag aaagtcccat
     9721 cacgctaccg cctattttca ataaagttcg ccgattgaac ttgtctgcaa taaccaaaaa
     9781 gagcaaagaa ccagcaacta aaatgattcc ttggatgatc ggccacatca atgcatcgct
     9841 cgctgcatgc ccagtcgctt tttctacgat cagcggaata tagtaaaaaa tagcattagc
     9901 tccttgaaat tgctggaata ctgctacacc aataccggca atgactaagg aacgatattt
     9961 ttctaaaaac aaagtatgcc aagaagttcc ggttgtcttt tcttgtttga cattcagctg
    10021 gatttgttcg aattcctgtt cagcttcttt tggtgtccga atatagttca atactctttt
    10081 tgcctcttcc aagcggccag attgcataag aaaacgcggt gactctggta gagctaaaac
    10141 accgaaaaat aaaatcagcg cggggacagc agcgagccct aacatcacgc gccaagccat
    10201 cgtttcaggc aaccctttta gcaaataatc tgcaacatag gaaaggagca tgccagaagc
    10261 aatcatcact tgattgatcc ctgacaatct tccccttagt ctagcaggag ccatttcaga
    10321 catataagca ggaactaggg cagatgcagc gccaacagct aaacctagaa gaatccttgc
    10381 tgcaatcaga aaataagaac cattgtgagg ggcagatgca gaaaggacag aaccagccat
    10441 aaaaataagt gcagaaagaa gaatcatttt tcgtcgtcca agcttatcag aaagaagacc
    10501 agataaagag ccgccaagga tcgcacctaa catgaccgaa gacgtgatcc atcctatcaa
    10561 agaagcttta cctgccaaac cccaatcatg ctgtaaaaaa ggtaaggcgc cggtcataac
    10621 accaatatca tatccaaata gaatgccgcc aaatgcgcca aaaaaataaa taaacgatga
    10681 ggaaactttt ttttcttttt tcataagtat tctctccttc tagaatttcg agaaggcggt
    10741 tgatcagatt ttgtaagcaa tttcatttta taacttatgt acgtacaaaa caagtgtttt
    10801 ttattgtatt cttgtaaata actgcttgac atgtacgtac aaaagtatac aataatcatt
    10861 gtaagcgcta ctaaaaagac ggtttgatca ggtttgacga gaaggaggga gttaaatcat
    10921 tatgaaggaa gtaaatgcgc aaagtatgtt agatgtcata gatggtcgta catctttagg
    10981 gattgagttt ggatcaacta ggatcaaagc tgtattgatt gattcatctt ttactcctat
    11041 tgccgctggt agttatgagt gggaaaacaa actagaaaat ggtatctgga cctattcatt
    11101 agaagatatt tggaaaggac tgcgcgtcag ttaccgaaaa atggcagcag aagtattcga
    11161 aatctatggc gagaatctta cgacaatcgg gtcgattggg ttcagtgcaa tgatgcacgg
    11221 ttatctagca tttgatagca aggaacagtt actagtacca tttcgtactt ggcgaaatac
    11281 aatcactgaa gaagcagaac gaatattaac ggaaaaattt caatacaata ttccgcagag
    11341 atggagtatc gctcatttgt atcaatctat tttgaataaa gaagaacatg tgaaagaaat
    11401 taattttttg acaacgcttg caggttatgt ccattggcag cttactggtg agaaagtact
    11461 aggcgtagga gatgcatcag gcatgtttcc tatcgatcct tcaacgaaaa cctaccatca
    11521 agaaatgaaa aatatttttg gtcagttaat cgaaaaagcg cacgtagcga ttgacttgga
    11581 tcacattttg cctaaagttc tagtagcagg agaagaagct ggcaagttaa gcgaatcagg
    11641 ggcttatctg atcgacccta cgggaaattt gagaccagga atcccggttt gtccgccaga
    11701 aggggatgca ggaaccggta tggtcgctac gaacagtgta gccaaaggga cagggaacat
    11761 ttcggctggt acttccgcct tcgctatgat cgtattagaa aaagaactag aaaatgtcca
    11821 tccggaaatc gacatggtca ctacaccttc aggagaacta gtaggcatgg ttcatacgaa
    11881 taactgctct tcggatatca atgcttgggt cagtattttt gaagaattta ccaattcttt
    11941 gggtatggag ataagcagag ataaattatt cacggtatta ttcaacaaag cgctagaagg
    12001 cgatacggat tgcgggggat tactttctta tggttatttt tccggtgaaa acattacagg
    12061 agtaaatgaa ggaaggccat tatttgtacg tacaccaggt agccgttttg atttagcgaa
    12121 tttcatgcgg atccatttag ccagtgcttt tggtgcaatg agaatcggaa tggatatatt
    12181 gaaatctgag aatgtccaaa ttgatcgttt agtggggcat ggggggattt ttaaaacacc
    12241 agaagttgga cagcggattc tagcatctgc tatggaggca ccagttacag tgatggacac
    12301 agccggagaa ggtggagcgt ggggaatcgc gttattagca gcctatatga tggatcgaca
    12361 tgggaagtct ttagaaaact tcttggcaga agacgtgttt ggacaagacc aaggaatcac
    12421 catcacacct tcagcagaag aaatagcagg ctatcagata tttatgagac gttaccgtga
    12481 ggggatcgac attgaaaaaa cagcgattca tcaattgcaa gaaaaaggag tgatagaaaa
    12541 tgctggaaca actgaaagaa gaagtatacc aagcaaatct tgacttgcct aaacacggac
    12601 tcgtcaaata tacatgggga aatgtgagcg cttttgatcc tgaaacacga tatttcgtta
    12661 tcaaacctag cggagtcagt tatgaagaga tgacagcaga cgacatggtc gttgtggact
    12721 tggacaatca tatcattgaa ggtaaactca atccctcttc ggatacgcct actcatgctg
    12781 ttttatatcg ttcatttcct caaatcggag gaatcgtcca tactcactcg acatgggcga
    12841 cgatctgggc acaagcagga ttggatgttc ctgcgatggg gacgacgcat gcagatactt
    12901 tttatggatc ggtaccttgt gctcgatttt tgacacaaca ggaaatcgac agcgggtacg
    12961 aatatgaaac aggaaaagtt atcatagaaa cattcaaaga acgcaaaatt gatcccttag
    13021 caattccagg ggtactttta cacggacacg gtccatttac ttgggggaaa gatgcgcaaa
    13081 gtgcggtaat gaacgcagtc gtactagatg aagtatgcaa gatgaattta tttaccagac
    13141 agctcaattc attttcagaa gagctgcctc aacggatttt agataaacat tatttacgaa
    13201 aacacggaaa aaatgcgtat tacggacaaa acaaataaaa ggagtggcta attatgttga
    13261 atattggaga aaaagaattt tggtttgttg taggatcaca gcatttatat ggggaagaag
    13321 cactgcgaga agtaaagaaa catgcacaag aaatggtcga tgaactgaat gaaaatggcc
    13381 aattgcctta tccaatccgc cttcaagaat tagcagttac tgctgatacg atcacaaaaa
    13441 tcatgaaaga ggtcaattat cgagaagaag tagcaggtgt gatcacttgg atgcatactt
    13501 tctcaccagc aaaaatgtgg atccgcggga caaagcttct acaaaaacca ttgcttcatt
    13561 tagctacgca atataacgaa agcattcctt ggaaaacgat cgatatggat ttcatgaatt
    13621 taaatcaatc cgctcatggt gaccgcgaat atgggttcat caatgctcga ttaaataaac
    13681 aaaataaaat cgtcgttggc tactggaaac gacctgagat ccaaaaagag attgctgatt
    13741 ggatggatgt tgcagtagcc tataatgaaa gttttggaat aaaagtggct cgttttggtg
    13801 acaacatgag aaacgtgggt gtcacagaag gagacaaagt ggaagcacag attcaatttg
    13861 gttggactgt tgattatttt ggtatcggag atcttgtaca agtaatcgat cgcgtatcag
    13921 acgaagaagt cgatcaatta tttgaagagt acaaggaatt gtataccttc gactatggtg
    13981 attatgaaga aaagacatgg gaggaacatg taaaagtaca agctcagcag gaaatcggta
    14041 ttcgccgttt cttagaagaa ggaggctata acgcttttac gactaatttt gaagacctgt
    14101 atggaatgaa acaactacct ggactagctg tacaacgttt gatggcagaa ggatacggtt
    14161 ttgctgggga aggcgactgg aaaactgcag cgattgatcg gttgctgaaa atcatggctc
    14221 gagggaaaga tacaggattc atggaagatt atacttacga gctagctagt ggccaagaag
    14281 caatcttgga atcccatatg atggaagtag atccgacgct tgctgcgacg aaaccaagaa
    14341 tcgttgtttc tcctttatca atgggagata gagaagatcc ggcgcgtctt gtatttgatg
    14401 ggaaagcagg agaaggggta gtcgtatcga tggcagattt cggtacgcat tataaactgc
    14461 tgatcaatga agtagaagcc tttgaaccaa cgactgaagc acctaattta cctgttgcac
    14521 gtgtgttatg gaaaactaaa ccgaatttcc atgaaggtgt ccattcatgg attcaggcag
    14581 gtggcggtca tcataccgtt gtctcgctaa atctaacaac agatcaaatt gaaacgtggg
    14641 caaaattagt tgagttggaa acagtggtta ttagataaaa agaggttgtg aaaaagccac
    14701 ttagttttaa gaaataagaa gagattgacg aaatttgctt ttcaactttt ttgaaattta
    14761 gtcttattat caatgtcagc taacggaaca ccatttatca agatatctca ccctcaatat
    14821 tttgctctcg aaaataaggc ttatggcagt aacttgtcat agccttattt ttatcactca
    14881 gcctactctg ctctaactgc tctttttgct tctaggaagt caaatggtaa acagttgtct
    14941 aattgcagaa atttcgagat acttgttagt aaggaggggg ttttatgtat cacgttcatt
    15001 tgattgatgc tattccagaa caagagatag aaaagttaca aggggaatat ttcaaaataa
    15061 tagatgattt gccagccgaa ggaatagtta ttcgtagtac gaaagtgaaa gatgaatggc
    15121 ttacaccaga tttgctagca atttcaagag ctggtgttgg ggtgaacacg atcaacgttg
    15181 aaaaagcttc tgaaaacggg acgatcgtca tgaatacacc aggtgtaaat gctaatgcag
    15241 taaaagagct tgttttgtgc tgtttgctct tatcttcccg tccgataatc gaagcaagtc
    15301 gtatggtaca aacactaaca ggtcctaata ttttagaaca ggcagaaaac aaaagaagtg
    15361 cttatgtggg acgagaattg caaggcaaaa ccatcggtct tcttggtctt ggagcgatag
    15421 gcacaaaagt agcgctaagt tgctatagct tagggatgga tgtcttgggt tattctatcc
    15481 gcgatgccca gttggattat gttcggcaag cagatttaga aacagttctt tctacctcgg
    15541 attacatcgt agtcatgctg ccgttgacag aagatacaaa aggattgatc gatcaggcga
    15601 atatagaaaa aatgaaaaaa gatgctgtac ttatcaatgt aggaagaagt gaaattgttg
    15661 ataaatatgc tgttatgcaa gcattggaaa aacaacatct ggctaaatac ttgactgatt
    15721 ttccagaaga agagttctta gaaaatgacc gtatacttat gttgcctcat ctagggggat
    15781 cgactcagga agctttggca gacagcggac gtttggcagt tgaagcctta aaagattatc
    15841 ttttgttcgg tacggttaga gaagcggtca attatccatc cgcacgaatg tttttccaag
    15901 cgccgtttcg attcactata ttttatcaaa agaaaaccaa tatattagca gagatatttt
    15961 ctctgctgaa tgagaacgag ctagcgatcg cagatatatc gcgtaaccat aagaatgagc
    16021 atgtctacat attgattgac atagacagta ctgattttga acagctcaat caagtgaaac
    16081 aacagctaga aaaaatttct ggagtacgaa aggtccgctt attaaagaaa ccagaaaggc
    16141 cataaagaaa gagaatattt ggatttcatt aattcttctt tccgttcttt actttcgaat
    16201 ttcgtacaac taaaataaga gaagcaacat tcttttattg atagaaatga aggagcaagc
    16261 atttgaaaag atatataagc agactttcga ccaaacagaa aaagatttta aaaggaacag
    16321 cagcaatcat cagtatcttt tttcttgttg tttttagtaa tttatttttg cagtggtgtc
    16381 aaaatgatct atccgtagac cttgcgttga aatttgcttt ttcttggcat acggaaaaat
    16441 tcttcttagc ctgtctcgtc ttgctgatta ttcttatttt tctgattgca ctagcaggat
    16501 ctgtcccttt aggttccctt acgtatgtcg tagccatcgg tgtattaggt tttgcaaatt
    16561 atatgaaaat gagttatcgg caagaaccga tttatccaga cgatttgaag atgatcactg
    16621 aaatcggatt gctgaaagat atgaccggca ctatgctgtt tacagtgatc ttagctgccg
    16681 caggaaccgt gataggattg ttttgctggt acatgtttcg aagcctgaaa aaaggaaggc
    16741 gattccaatt gatccgatta acgacactgc ttgtggcaat tggactgcta gggtatatca
    16801 gtaatttcaa caatcccgat aatttgttgc gaaaagcata taataaaacg gcactatgga
    16861 taccttacag ccagaaaatg aactactata acactggttt tatcggtggg tttttgtaca
    16921 atctgaaggt agaaccaatg gatgaacctg aaggatattc gaaagaaaag atcaaagaaa
    16981 tcactgaaaa ataccagaag ctagccgatg aaaaaaataa aactgtagag gaagagtcgc
    17041 cgaacatcgt gttcgtgatg agtgaaagtt tctcagatcc tagtcgatta aacggcgtag
    17101 aagtatctgg tgagccattg gcagattatt atgaagtagc tgatcaaaca tatagtggca
    17161 atatgctttc tcaaaactat ggcggtggaa cagcaaatat cgaatttgaa gcattgactg
    17221 gtttttctat ggcgttattt aatgcgcaac tcaccacccc ttacacgatg cttgtaccaa
    17281 aaatggatca gcttccttct atcgtttcag cgttaaacgc acaatcatac cagacgacag
    17341 cgatccatcc atacaatact tcgatgtata aaagagagga tgtttatcag acattaggat
    17401 ttgaccagtt catcagcgag cgtacgatga cctatacgga tacgatagag aataatccat
    17461 atatttctga tgagtcagct tacaaagaag taatgacact tctcaaggaa gaaaaaacac
    17521 ctcagttcat tcatttagtc acgatgcaga cacatatgcc ctacgatgga aagtatgata
    17581 atctagacta tcctgcaaaa acttcagatg gaagtgggac acttgactta gagaattatc
    17641 tgcaagatat ttcctatagc agcacggcac tgaagcagtt cacggaagaa ttgaagaatc
    17701 tttctagaag gactttagtt gttttttggg gggatcatct tccagggatc tattcagata
    17761 cgatccaagc aaaaaatgac aaacaaacat tgcatgaaac acaatttctt atgttcgaca
    17821 gtaaaggaga attagaaaag caaacgactc acgatgcaat caccagtccg ttttattttg
    17881 cagcgaatct aatggaacag acgaatcaga caacaaatgg cttttatcag ctgcttttat
    17941 cattagaaca ggaacttccg gcgtttgaac gagaactgta ctatcaaaac ggacagtggt
    18001 ataaagaagc gcaattcaat cgctcgcaac aagagattta cgacgaatat caattgatcc
    18061 aatatgacat tgtcgcaggg aaacaatata gtttagcaga tggtttcttt gagcacgaat
    18121 aagaatggcg agctgtatag aaaccattaa attttttcaa gtacttgtca tttgaggata
    18181 aaaacaagta gaataaataa taacaacaat ggagaaagta ggaaaacgaa catgttaccg
    18241 aatattggct attatgtgca gtccatcgat caaatcgtca aggaaacaga agcaatcgga
    18301 gaaggaatgc atccgaaata tgaagtgatc agaaacgcaa ttgatgaagg aaaaacaagt
    18361 gaactggaca aatcgactct tgaagagatc atcctgttat ttgaagaagg aacaaaaaaa
    18421 tatcgggcaa tgcttatgaa agtcagtact ttgcgtccac cagcaaaagc aatgggcatt
    18481 cataaaaatt ttgaaaaagc ttatctttca tatgtagcag gctgtgaaga aatgatccaa
    18541 agtatcgatc ttgaatcagg ggtagatgaa gatctattca atacatctga agcaaaacaa
    18601 gaccaagcga cagaccaatt gacagctgcc atccaaaaaa tgactaattt gttgttgaaa
    18661 aaataaaaga aatctttgat aacaataaaa actagagtta tctaatataa atgtaatatt
    18721 acctttatga tagagtgatc aaaatccttt atgataatga catacctata accaacaaca
    18781 accttgtact tactcaaaat cgagtaaata ctccttttcc gtcgagtcta accatctcgg
    18841 cggtttttta tttgtagaaa agaacatcag gatagacgga aaagtcttgt cctgatgttc
    18901 ttttcagata gtatggaaag ctgattagtt ttctacattc tgtacatatt gttcaaagta
    18961 gttctgtaaa tcggctttga gtttctgata ttcctcttcg ctatactctt tatcttcgat
    19021 tttcccatca aagaaaggaa tatttagttc ttcccgtagt ctgtctaata cgtcttgctg
    19081 attcatttca ttctcctttt cttactaacg atcaaaataa acacagacac attctggtac
    19141 atagacatct tttttcagta attccagtaa tcctgtttca ggatgacggc gatataatgt
    19201 cagattatcg gtgttctgat tggcacacac gacaaaatta ttgcttggat ctagtgcgaa
    19261 atcgcgggga aaatcccctt ctgttgagat gatttgcttg ttttgaatgt gggaaccatc
    19321 ttcaaaaact gcgaatacag caatcgagtt atgtcctcgg ttagaagcat aaaggaactt
    19381 gccatcattt gaaatgcgga tagctgcccc tccattttca ccgtcgaatt cttctggcaa
    19441 ggtcgatatt ttctgtaatt cttcaaaagc acctgtttct ttattataag aaagcacgcg
    19501 gactgtgctg tctaattcac caaataaata agcgaattgt ccatttggat gaaagacaag
    19561 atggcgaggg cctgttcctg gctctgcagt gaaggtagtt acaagattca atttgccgtt
    19621 ttcggacaca tcataagtat acacacgatc tgtaccaaga tcacatacga ctaatcgttg
    19681 gtcaggagtc agatccgtat agtggacatg cgcatgatct tggtttttat gaggacctgt
    19741 cgcttcttga tggaatattg aatctgctgc tttcagttcg ccatctccta atatatggta
    19801 gacatttact tcacctttat gatagttcgc cccgtatagt aattgacgtg cttcatcaac
    19861 cgctacataa cacaaaggag cgccagactc tgttaccgca ttcaaaaaat gaaaatcctt
    19921 atcgtacgca ccggcaccac ctaatccatc cacactagta actgtgtaaa gattgccagc
    19981 tttacttttt gctacatagg tagggctatt ttcttcaaca gctaatgaaa ggttggctaa
    20041 ttcttctttt tctgtatcaa ggtcgatcgt atagattcct ttactttcgc gacgcgtata
    20101 ggttcctaaa attatctttt cgatcatatg agctgattcc tcctaattgt aatgaaatag
    20161 ttttcgtttt tattctatca cataattatt ccacacggct aaagaatgag aatgtgttat
    20221 actgtttttg aaagcaatta aaggagagag tgcaatgatt caggcaaaga ttacagaaat
    20281 tgggacagaa gcaataaaca aggaagaacc catgctgatt ttgttcgata aaacagcaac
    20341 agctgcctta cgaaaatatt cggtgattca agaagtatca aaagacgaac ctttttcttt
    20401 gaaagcaggc ggaacaattg cttttgatga tcaggtatat acgatcgatt atgtcggtcc
    20461 gatggccaat gggaatttaa agacagtagc tcacgtatca ttgatttttg atgcttgtcc
    20521 agaagaagga caaattgcaa atggaatcta tcttcatccg catagactgc ctgctattgg
    20581 tttgggaagc caaatttttt ataactaaca ggaggaatta tggaaggaaa aaataagaag
    20641 aacagaccgg tatcgtggtt ttggcgctgg tttttaaata atcaagtagt cacagcctta
    20701 cttgttgtat tgttaatatt gctgatcgtc tttttattta caaaagtttc atatttgttt
    20761 gcaccaatct ggcaattttt agcaatcgta ggtctgccta tcattttagc aggaatcttg
    20821 tattacttga tgaatccggt agttgattac tttgaaaagc gaaaagtacc aaggctttac
    20881 agtatcattg ggctgtttgt tttggttgtt gcactaatcg catgggggag cgtagtgatc
    20941 atccctaaga ttcaggaaca aacagtgagc ttcatcagta acttccctca atatgtagat
    21001 acgattgata ataaattaac cgagattttg cgggacccgc tgttcaacca atttcgcgaa
    21061 cagttggaaa cagcaggtga taagttcatg aattcggcag gcgatatgat ccaagatata
    21121 tctaagagta cagttcagag tcttggaagt tttgtcggcg cggtagcgac tattttagtg
    21181 gctctgctga cgatgccgtt tatcttattt tatctattga aagacggtaa acaattagca
    21241 ccttattttg tcaaattttt accgacacgc atgcaaaaac cgacgttgaa tgtgctgagt
    21301 gaaatgaata gtcaagtttc ttcttatata agaggacaac taactgtggc ttttgctgtg
    21361 gcaatcatgt ttatgattgg ttttgcggtc attggtttag agtatgctgt cacgttgggg
    21421 attattgcgg gttttttaaa cctgatccct tacttaggat catttttagc gatgattccg
    21481 gctatctttt tagccattgt agcaggtcca ttcatgatca tcaaagtact gatcgttttt
    21541 gcagtagagc agacaataga aggacgcttg atctctccat tagtactagg gaatcagtta
    21601 gcaatccatc cagtaacgat tttattagtt ctgctgactt ctgggaaatt gttcggaatc
    21661 atcggtgtga tcttggggat tcctgtttat gcagcagcta aagtagtgat tactcatgtt
    21721 tttgaatggt acacaacaat ctcttctctt tatgaagaaa atccgttgaa aaagcagaaa
    21781 gaataacaaa aagagacaga tgggaaaata tccaaggcac aaatgctgct ttgaagattt
    21841 tttcctctgt ctcttttgta tgcatttatc tcaattttta aaatgattta cctaaagcag
    21901 cagctttatc taatgcattt tgcatcaact catctgcacg gtcagggtca taatcgattc
    21961 cttcgatgaa tagctgatct acttgatcga tgcctacaaa gtttaaaata ccttttacat
    22021 attgagaggc aaagtcttgt ccgttgtata caccgccatt agattggatg tgcagtgctt
    22081 ttttacctgt cactaatcct tctggcccgt tttctgtata gcggaacgtt tttccagcta
    22141 cattgacggt atcgaaccaa gcttttaatt ttgttgggat atttagattc cataaggcgt
    22201 ttgcgataac gattttatca gcagcaagga actgatcagt caattcattg aaacgagcaa
    22261 ttttttgttg ttgactttct gataaggaag tgaattctgc accagcacgt aatgcttccc
    22321 aacctgataa taattcttca tcgatttccg gaatatcatc gcgataaagt tccaaaacag
    22381 tcacttcatc atctggatga ttttctttat aacttgttaa aaaagacgtc aatgccttaa
    22441 ctgtgcgcga ttcttctgca gttaggggat gacctttgac tactaaaaca gatgccatat
    22501 gcttaccttc tccttttatt gctatgattg ttataactat gactaacaaa atcagtttac
    22561 tcgaaaaaga aaaggctatc aagtcatatt gataaggttg aagaataaat aactaaaaaa
    22621 agacagggta taaaagaggc tgtgacacga ttttttgtca cagcctcttt tgaatctaac
    22681 aagcaatatt caagcaacac tcgactgaag agcttccatc ccaccaatga tataaacagc
    22741 acgaaaacca ttagcagtaa gttgatcggc taatgcttct gatcgcctgc ctgtatagct
    22801 tagaatataa taaagtttct ttggatctag attttttaat tgtcttggca agatagcagc
    22861 ggggatagaa agcgagtggc ttaagtggtt ttttgtgtaa tctaaggtat ttcttgtatc
    22921 taaaacgatg ctgtcagcat cgagaggcaa tttattgaag tcttggacac taattgattg
    22981 ggccatttat ctcatccttt gtttagtatg tggctatttt aatgggcaac tctcattaat
    23041 tcaaatggaa ggattcctct tatcactttt ttaatgtatc ggatgataat gaaaaaaact
    23101 atgcaaatgt cttgcaaaac aggtaggagg tgttataatg acatagcgtc atatgacaat
    23161 gtgtcatctt gaaagaaagg agaatccata tgccgacaca gacatttttc catttgccta
    23221 aagaaaaaca aaaaagacta atcgaggctg cgagaatcga gttttctaga gtacccttaa
    23281 aagaagcttc gattgctaat atcgtcaaat tagcggatat ccctagaggc agtttctatc
    23341 agtattttga agataaagag gatctttatt tttattactt tgagacaatg agacgtgaca
    23401 gctctaaaga tatgttacaa ctaatgaaag aatccgatgg tgatctattc aaaggattcg
    23461 aatcctattt ttctaaaatg atttatgaga ttttggaagg cgagaatgct tctttttatc
    23521 gtaacttgtt tatgaacatg gattaccgtg cctcaagaaa agtcgctcct catacgagtc
    23581 gccaaaaaac gcctcacgat ccacataaag aacacaaaca agatgcaaaa gagttgcttg
    23641 aattgatcga tcggacgaag ctgaaagtaa aaaatgatca tgaattggaa ttactgatcc
    23701 aattagtgat gaatactgta tttacaacaa ttgcgcattc gtatcgctta ttttcaacat
    23761 ctgaagatta ctcagtagag caagcaatca gtgaattcaa tttgaaactt tcttggctga
    23821 aaaacggagt atataaatag aaagaaggga actagatgct aaaaatcgta aaacggatct
    23881 ccttgacttc agcaattgca gctgcagttt tcatggtgat ccaagtactg gcagatttat
    23941 atttgccgac acttacctcc aatattattg ataaaggggt agcgcaagga gatgtcgatt
    24001 atatttggca tacaggtttt gtgatgatcg gcttttcttt gatcagtatc ttagctgcta
    24061 tcgcaaatac attttttgct actcgtgaat cacagaaact gggtaagcaa ttgcgtacgg
    24121 atgtctataa aaaatcagag agtctgacaa aagatgcgtt tgataaatat ggaacggcct
    24181 ctttgatcac aagaacgacc aatgatgtga ctcaaatcca aatggttaca caaatgtttt
    24241 tacgtatgat gatcaatgca ccaatcacgt tgattggcgc gagcatactt gcttatcaaa
    24301 aagatcatca gttgacaaaa atcttcttag ttgtcatccc agtaatgatt attttgatcg
    24361 gcggaatcat gtactttgct gttccattat tcaaaagtat gcaaaagaaa actgaccgtt
    24421 taaacctggt atttcgagaa ggattgacag gggtcagagt gattcgagca ttcgataaga
    24481 cacgctttga agaaaatcgt tttgatttag cgaataaaga ttatacaaat acggcaatca
    24541 aagttaatac gatcgtcgca ttgatgatgc caatgatgac attgatcatg agcggtacaa
    24601 acgtagcaat cacttggttt ggtggacact acatagcaga tatgactttg gaagtcggta
    24661 atctgattgc atttatgact tatgcgatgc agatcttgat cagctttatg atgctttccg
    24721 ctatttttat catggtgcct cgtgcacaag cttcagctga ccgtattaat gaagtgttgg
    24781 atgaaaaaat cggtatccat gaccctgaaa atcctaaaac agtttctttt gctggaaaaa
    24841 acgcaacatt ggccttcaat catgtcaatt atcgatatca tggagcagaa aaacttgcat
    24901 tggaagatat tgatttccaa gccaagtcgg gcgagatcgt cgcaatcata ggggggactg
    24961 gttcaggtaa aacgacctta gtcaatttga tccctcgatt atatgacatc gaatctggtt
    25021 cgatccaaat aaatggaacg gacattagtg acatgactca atataatttg cgagaattaa
    25081 tgggctttgt tcctcaaaaa gcagttctgt tctctggaac gatccgagac aatatgcagt
    25141 atggtaagcc ggatgcaaca gatgaaatga tttggaaagc attagaaatc gcacaagcta
    25201 aagattttgt ttcagagatg gaagatggct tagatagcca tgtggaacaa ggaggaggaa
    25261 acttctctgg tggacaaaga cagcgtctgg caattgcacg ggcattagtc aaaacagcag
    25321 atatctatgt gtttgatgat tccttctcag cgttggattt caaaacggat gccaatcttc
    25381 gccaagcatt gaaaacgaat atgaaagaaa gtatcaccgt tttagttgct caacgagtaa
    25441 gtacagtaat ggatgcagat atgatccttg tgctagatga aggaaaactg gttggcaaag
    25501 gaacacatga agaactatta gcaacaaacg aaacttatca agaaatcgtt cattcacaac
    25561 taagagagga ggacttagca tgagtaaaac aaacacacgt ccctctcatg gaccagcagg
    25621 cggtccagga catggtggac ctggcagaaa tctcggtgta aaaggtgaaa agcctaaaaa
    25681 tttctgggga acggtacgcc gattgtttgg ctatatgtct aaacgtatgg ttgcgatcat
    25741 cgctgtattt gtactagcaa tagcagccgt gatcttccaa atccaaacac caaaggtctt
    25801 ggggaaagca accacagaaa tattcaaagg tgtaatggct ggcgctcaac agatgcaaca
    25861 aggccaacaa gtaacaaaat ttccgataga ctttgataaa atcgcgcaga tcattgcgac
    25921 agtaatcgtg atgtatttga tttctgctgt attcaacttt ttacagcaat ttattatgac
    25981 acgtgtttcc caacgaacag tttatgaatt acgtcatgat ttggaagcca aaatgaatcg
    26041 tgtaccgatt tcctattatg atacacatac aaatggggat atcatgtctc gagcaatcaa
    26101 tgatatggat aacatcgcaa gcacgctgca gcaaaatttg actcaatttg taactagtgc
    26161 agtcacattg attggggtca tcgtgatgat gctttcaatc agctggcaat tgacgttagt
    26221 cgcgttattg atgatccctt taagcttgat catcgtgatg atcgtagcgc cgaaatcaca
    26281 agtgtttttc gcagatcagc aaaaaagttt aggtttattg aataaccaag tagaagaaac
    26341 atatggtggt cataccattg ttaaaacatt caatcatact gaaaaagacc aggaagtatt
    26401 tgaaaaagaa aatcagaatc tgtatgccgc tggttggaaa gctcaattta tttcggcaat
    26461 catcatgccg ttgatgaatt tcgttaaaaa tctaggctat gtgttcgtcg ctgtactagg
    26521 cggagtaaaa gttgcaaatg ggaacatgac attaggggat gttcaagcgt tcttgcaata
    26581 tacaacacag ttctctcaac caatcactca acttgcaagt ttgatgaaca ctatccaatc
    26641 aacagttgct tcagccgaac gggtatttga agtgttggat gaagaagaga tgtccaatga
    26701 aaaatctggc cttccagaag aaaaagatac accatacaaa gttcgttttg aaaatgttca
    26761 attcggctac acaccagata acttgctaat gactgatttt aacttggatg tcaaacctgg
    26821 tgagatggtc gctattgtag gtccgactgg tgcagggaag acgacactga tcaaccttct
    26881 tgaacggttc tacgatgttt ctggaggtag tattcgttac gatggtgtag atacccgtga
    26941 tctttcacgt gatgaattgc gcgcacagtt ttcaatggta ctgcaagata cttggctgtt
    27001 tacaggaagt atctatgaca atatcaaata tgggaacgac gatgcaacag acgaacaaat
    27061 cattgaggct gctaaagctg ctcacgtaga cgattttgtt cgtaaattgc cagaaggata
    27121 taacactgta ttaaacgagg atgcaagcaa tatttcgcaa gggcagcgtc agctaatcac
    27181 aattgcgcgg gcattcttag caaatccaga tgtcctgatc cttgatgaag caacatctag
    27241 tgtagataca cgaacagaaa tactgattca aaaagcgatg aaccgcctat tggaaaaccg
    27301 gacaagtttc gttgttgctc accgcttgtc tacaatacgc gatgcagaca acattattgt
    27361 gatgaatcat ggttcgatcg tagaaacagg aaaccacgat acattgatgg aaaaagacgg
    27421 attctatgca gatctttata acagtcagtt ttctgaagaa gaagcttcat agaatagtac
    27481 aaatttatta ttttttttag gggctgtgac aaaagtcgtg tcacagtccc tttttccgaa
    27541 taaacggtgt tcaatcagct gtccttcggt attagctcaa aaaacggcaa aatatgagaa
    27601 gctatttttc cgttttgtat ctctaatact caggtcaaac cgctgatttt acaacctctt
    27661 ccttaaacgg tgttctcctg ccaactcctc ggtcacaagt cacaatagtc aacaaacctg
    27721 agaagctgtt ttttgaaatt gctccttgtg actcggagtt aaacggcagg ctcacaacct
    27781 cttggtaaac ggtgttcaaa agccagtttc tcggtattaa gccaaattta tgcaaaaatg
    27841 tagaaagcca ttttcacaaa atttttctta ctactcgaaa ctaagcgact ttttcacaac
    27901 ctcttcctta aacggtgttc aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa
    27961 aataggagga gctatttttg tttggctgtt cttattactt gatgcagaac agctttttca
    28021 caacctcttt ttataagagt ttttactcat taatttacaa aaaacaagca aaatgcgaat
    28081 tttctcacat tttttttaac aaaatcaaag ttattgcttg aaagaagaag aatccatcgt
    28141 taaaataaag cttgacgtag aggggagcaa gaaatatgaa agattttcaa ttgggggctg
    28201 ccacctattt aaaaagtgta gccattcggg tgaaagaccg agataaaatg attgattttt
    28261 acaagaacgt tgttggcttt gatttgaaaa gagaagaaaa cgaattagcc attttaggaa
    28321 cagaagaagc tggaagtgaa atgttgctgc tggaagaaag tcctagagcg aatgatcaca
    28381 tgggggtgat caaaaagctg aaccgttttt cattgattat ccctagcaca gaagaaatgg
    28441 cagatatcct ttgtcgtatc cgtaaaatga attatccaat caaagaagct ttagaagatc
    28501 atggacgtat ggggattttg ctcgtagacc cagaagagaa tgaattggaa atttaccatg
    28561 aagagaataa gaacgaccaa agagaaattc cagaaacaat ggatcaagaa gcattattaa
    28621 gaaaatctga agaaaaattc agtaaactat caacaggttc ttattttgaa aaaatccatt
    28681 taaatgttac taacttaaca aaagaacgcc aatttcttaa tcatgtactt ggtttgaagg
    28741 tacataatga agaagagggc atccatgtat tgaatgaagg agatttccat gttggtttga
    28801 cagaagctag cggaggcgca attgatttgc ctacagatga agtgattgga cttgattttc
    28861 tgaaattcat gatcgatact gaaagcatgg aaggactgat tgctcacttg cagcaactat
    28921 cagttgattt cttcttagac aaaaagcaca cagtactcac aatctatgat gctataggaa
    28981 tcgaatggtg gttcgtcagt gagccaaaag aaaagaataa aactgacatt cgatgcgacg
    29041 cttaactttt tctttcttaa cgattgtcgc atgtctcgtc attttatgaa cggtcatact
    29101 atcgcttatt ctagagatag aaagagaaaa tagtataccg ggggaaactg cgcggttttt
    29161 cgcgcagttt ttttgtgtaa aaaaggttga ttcctcatag attaagcttt aagcttggac
    29221 tgtaagaatc tgatagaaaa gcaggagaaa aagatgtatc cacacacgaa acagcttatt
    29281 ttgaaaaaga tggaagaagg attttatcca ggtgtagcag tcgccttcat aaaagaaaat
    29341 caagtagaaa catttacagc cggctttgcc caagtcgttc ctgaaaagca gctgatgaca
    29401 gaagatctgt tgtttgatgc agcctcactg acgaaagtcg ttgcaaccac ttcattagtt
    29461 ttacgtctat tagaagatgg aaagatagaa ctcgatcaac cgcttcataa atatcttcct
    29521 gaatttgaaa atcagacaat cactttgcgt catttactga cacatacttc tgatatcaaa
    29581 acatggatac caaacagaga taagctatct caggaagaat tgatcgccgc ttatttgaag
    29641 ctagaaccag gtgagcaact aggaaaagaa gtaaaatata cagatgccgg tacgattctt
    29701 cttgggttga tgctagagga aatttaccaa gaaactgcag tggaaatctt tcaagaacaa
    29761 gtattggaac cattaggaat gatgaacagc ttatttcttc cttttccttc caaaaaaatc
    29821 gttcccacag aacaactgcc ttcgggtgag gttttgcgtg gacaaactca tgatccgaaa
    29881 gcaagagttt tggctgaaca tggaggtaac gcgggtctct ttattgattt gaaagacagc
    29941 attacttttg tccaaatgta tttgaacaag ggaaaaatcc gtaatggtcg ttttttagaa
    30001 gaagagacga ttgattcttt actgcaagat caaacaccga aaaaaaatgg ctatcgttct
    30061 ttaggatggg acttaaaaaa ggatgtagaa gacggtcatg cgattctttt tcatactggt
    30121 tatacaggta cgtttttctt gattgatata aaaacacaag aagcgttgat atttttgtca
    30181 aatcgcgtac atcctgtcga tcatcgtgcg gagtatctca tatgtcgaga tgaaattgtt
    30241 gaaaactttt tgaaagagaa agcggtatta ccgaaataat gctataatag ctatgaaaaa
    30301 tgagaggagt ttgaattatg caggaaccta tgtttttaaa accagttttt caagaaaaaa
    30361 tttggggagg aagtcgttta cgttcagtct ttggttttga tatccctaac gataaaatcg
    30421 gagaagattg ggcgatcagt gcgcatcctc atggtgtcag tgtagtagaa aatggcgaat
    30481 tcaaagggaa aagattagat gaactatggt cagaacacaa ggaattgttt ggccatcctt
    30541 cagaaccagt gtttccatta ttgatcaaga tcttagacgc agaagatgat ctatctgtac
    30601 aagtacatcc agatgatgcc tatgggatga aacatgaagg tgaattagga aaaactgaat
    30661 gctggtatat catcgatgct gaacctggtg cagaaatcat ctatggtcat catgcaaaga
    30721 caagagaaga actagctgag atgatcaaag atgggcgctg ggatgacctt ttgaagaaag
    30781 taccagttaa aaaaggtgat ttcttttatg taccaagcgg aacgatccat gctattggta
    30841 aaggaatcat gattttagaa acacagcaaa gttctgacac gacttaccgc gtgtatgact
    30901 acgacagaaa agatgcgcaa ggacaaacaa gagagctgca tatccaacaa tcgatcgatg
    30961 taacaactgt ccctgcgaaa acaccagaat tacaaatcaa agaagtccga aaagggaatt
    31021 catcgatcgt cacttattta gaaactgagt ttttcaatgt atatgagtgg gatattaaag
    31081 gaatcacatc tttcaaaaaa caagcccctt atacattgat gacggtaatc gaaggagcag
    31141 gagaccttgt cgttgacgga aaaacatatc cattagaaaa aggaacaagc tgtattattc
    31201 caaatggcgt ttctgagtgg gcggtccaag gtgaactagc aatcattgcg tctgaaccag
    31261 gcaaatagtt cccagatagc tgaaagcagc agacggtttt tcgtttgctg ctttttctac
    31321 ataaaatccg tccatcaata aatagaagtc tggtgaaaaa gatggaatac aaatggaaag
    31381 caaacaacag cagtcgcgtg tctatgaaac aactgagaaa agctggacat cacttaaatg
    31441 aaatgatcat gccaaatcta agtctgttta tcgcttgggg aatcttgtca ttagcaacaa
    31501 aaggtgtgtc tggcgatttg cacaccacat tagaagaggt atgccattgg atgatccagc
    31561 tgcttttacc catcttgatc agttattcag gcgggaaatc ggtaggggga caaaaagggg
    31621 cagtcgccgg tgcaattgct tctttagggt tgatcgctcg ttccaatgct ccacaaatcg
    31681 taggagcgat gattatcggg ccgcttgccg gcatcgtata tgaacaattt gtttctagat
    31741 tccatgtgaa attcaaggct ggatatgaaa tgcttgtcag caacctacta gtaggactga
    31801 ctggtagcgt tatttgtata attggaatcg tttttattga accagtttta aatagtgttc
    31861 acttgttgct cgcctccgtt gtgtcttggc tagtcacaat gaatctgtta ccacttgtca
    31921 gtctgatctt agaacctcta aaagtactat ttttcaataa tgcaatcaat catggtgtat
    31981 tgacaccttt aggaatcgaa ttctcacaaa cagtcgggta ctcttcctta tttttgatgg
    32041 aggcaaatcc tggacctggt ttagggatct tactttctat cctttgtttt gcagaaaaac
    32101 aggaaaaagt aaatgcgagt ggagcattga tgatccatgc gattggcggg attcacgaaa
    32161 tctattttcc atttgtcttg ctgcgaccgt atctattttt agctgtaatg gctggtgggg
    32221 caagcggcac gttgatattt caatggttag atacgggatt agctgcacct gtatcgcctg
    32281 ggtcactact cgtgattttt gccaatgcgc cttatccaca actagctggg gtaagtatag
    32341 ggatagcaat cagtacattt gtgaccttca tttgtgcagc gatactattg aaattaccaa
    32401 ataaagaaaa agagaacaaa acggtcaaag aaaggggaga aatcttggaa ccatctatca
    32461 ttcattccgt catttttgct tgcgactcag gtatgggatc aagtgctatg ggagccgccc
    32521 tgctgaaaaa gaaattagca gcagaaaaaa tcgatctttc tgtcggatac agctctatct
    32581 atgaattgaa taaccaagta aatcagctgg ttatcgtgca acatgaacta gcgggtcttg
    32641 caagagaaaa agtacctcaa agtcaaatct atgtactgga tcactttttg gaaacagata
    32701 tcttgctgtc acgaataaaa gaagcgatcc aatttccaaa gcgggaagaa aaaaaagaga
    32761 tagcagatat cacagaacaa aaaacgtcgc ctgctaatat tgtatttttg tataaaaaca
    32821 acagacgcgg ctcacaaact atgggcatga cgattttaag tcaattagca aaaaaagcga
    32881 aaaaacagtt tgttatctca aaagaatcaa tagaaaaaat caaaatccaa gaagaaacga
    32941 tttatatcgc acaaagagaa ctagctgtaa ctgaacagct agaagaaaag atccctcggc
    33001 tagttctagt tgaacattgg gtagctacga atgaatacga aaaactcgtg aagggagaaa
    33061 tataagatgt atttatcgcc cagagaaaaa caattattag cagaatttct cagtagcccg
    33121 tcacctgtat ctatccaaaa aatgatgaat ttattgaagg tcagtaaacg gactatttac
    33181 cgcgaattag ataatcttga agtctcattg aaatcggtaa atgcttcttt gaaaaaagca
    33241 gctagaggct catttgtcat tgaggcatca gaagaagtat tagagcaatt aaaagaaaat
    33301 acagatggga aaagtaatga cttatccact gctaaacgac aacacgcgat tttagccgag
    33361 ttgttactga cgacagagcc gttgagtctt gctcatttca tggaatcata tcgcatcagt
    33421 aataccactt tttatgcaga tatcaaacag ttggaagaaa gtattcgcca actgcctttg
    33481 gaaatcattc ggaaccaggg atacgagatc gaaggaccgg aaaaataccg ccggcttctg
    33541 acagcgaatg tattagaatt agaaatcaat gaatatgaac tttttcatag tatttctttc
    33601 gatgcatcac tgaattattt ttttcgattc gttgatccgc aacatctatc tcttgcacgc
    33661 aaagtaatcg gggaagaatt gatccaaaaa aagaccaatc taagtgatag aaagcttgaa
    33721 catttggttt tgatgctgac attgacgatg gagcgtgtga cgaaaaatca tttactgaca
    33781 aatgagacat ataatagtct ggcaaacaaa gaattactac agacagctaa acgattgttt
    33841 tcacggatcg cttctgagac aaaacaactt tatccagtaa atgagatcgt cttttttgca
    33901 ggattattaa atgacttcac gaactcattt gatcagaatt tctttgaaga gacgttcgat
    33961 acacagcttg cttatttagt aaaacaactg attcaagaag taagtgaaga gacgaatgtc
    34021 catttttatg aagatgaaac tttgtacaaa atgctgttga ctcatttatc tggtgtgttt
    34081 tcaagagccg ttttacaaga agagacactg accaatccga ttttagaaaa aatcatggtt
    34141 caatacgagg aagttgcgaa tgcaatccgt aaagcagtag agcggatttt tcaagaaaaa
    34201 aaattatccg aagaagagat cgcttatatg gtccttcatt ttgcgaattc attggaacga
    34261 agtccgaaag aaagcaatat cagtattgca ggattttcgc caagtggatt agcttctact
    34321 agcatgttgg aactacgatt gaaaaggtat tttcccttca tccaacaaat tcatttctat
    34381 cggattgctg atttaaaaaa aatcgattta caagaagaat atgatgttgt tatttctaca
    34441 tctattttac caggatacgg tggaaaatat attttagtct ccccgctact gctagaagat
    34501 gaagtcaagc agctaaaaga agctttttcg catattgaga agaaaaaaag gcaaaaaatt
    34561 acgtataaca gcaataacga gtggatgata gaagatactt atgaggaaac aatgcatttc
    34621 atggatcaaa tcaatcaatt actcggtcgt ttttttatcc aagaactgga taatcctgag
    34681 acgatttccg aaacgattcg cagcttattt ccttattttc ctgaaggact agtaacggat
    34741 gaagaggtgg ttcatcgccg gctgcttaag cgatatgaac aagcacctat cggtattcct
    34801 catacaaata tggggttatt ccatacatcc agctcattga tttctaaacc gatcttttgt
    34861 attttcaatt tgaaacatcc gttgaccatt gaagggatgg atagacagcc agtcgatttg
    34921 tcccgtatgt tagttatgct ggcgccttcg ccaatccaag aaaaagacgg gattctttta
    34981 ggaaagatca gtggagcaat catcatgaat gacttgaaca ccgagatctt ccgctcagga
    35041 aataaagaaa tcgtttatca gttgttagcg aaaatcctga ttgaagaaat aaagaaatga
    35101 aggtgaaaag atgatacagc taccagcaga aaaaatattg ctcgatcaat cctatcaaaa
    35161 ttggaaagaa gcgttgaccg agatcggcca gtggatgacc caacaagggg gaatcaaacc
    35221 ggcatatatc caatcaatga tcgaaagaca agaaaaagcg agtgtctata tcgggaattt
    35281 cgtagccttg ccacatggga ctgacaaaga tgaagaagta gtagaagaag gaatctattt
    35341 agtccaagtg cctgatggtg tggactttgg aacaaaagag gaaccaaaaa tcgcgacact
    35401 cttatttgta gtggctttaa aaaaagaaca tcagttagcg gtgctccaag atttagcctt
    35461 tttctgttca gacatcgacc aagtaatggc gctcagtgat gcggaaacca ttgaagaagt
    35521 ccaaatgatt tttgaacatg gacatatata accaatgttc acagtaaaaa aagctgcctt
    35581 gcatcaagta ataacttcaa ccaagataaa aataagagga gctatttttg ttgggaggtt
    35641 cttattactg cggatgcaag cttttgaaaa ctatttattc tagaaaaccg tttatttgaa
    35701 ataggggacg gtgacaaggt ttttgtcacc gcccctatta tttttgtgtt ttaatagctc
    35761 ttttttatat agatagtgcc catacctatg aataataaaa cgatactcca tataccaagt
    35821 gtcaagatac cattcatgaa agtaacctgt tctgaagcta gtggcgagaa aatatctcct
    35881 cctatgatca cattttgaaa atcaacataa gaagttggca agtaatgact gattgattct
    35941 atccatccaa tcgaagaaaa caagcgatac tctgctaaga aaagacaaag aagtgtacct
    36001 aaaagattga ctagagaatg ttcaaataaa gcagatagaa gaaaactgat tgtacttaaa
    36061 aagagcaccc acaacaacag gaacagaaag ctctggaaaa gatactcata catcggcagg
    36121 attcgtatct cctgaccaat gatcgttcct acaggataac ggaagtctcc caaaccattt
    36181 ttgattatta ctagtataga gacgatcaaa aatggcatca cgagacttag catagctgca
    36241 ccataataaa tggtaaaacg gatgctatgt ttctttacca tactatccgg taatagattg
    36301 actagagaga ttacctgctt tcttttttca taagagatga tggaagaaag aaacaaggcg
    36361 gttatacata aaatcaaaaa actagaaatc attcctgaaa aaatattttc gtagtaattt
    36421 gctaaagaga gatgcgctgg caagtcaaaa atagaatatc tttggatctg atgttctttc
    36481 atataacgga gcaactcgac acgtttttgt tgttcaatga ttggaattcc ttgtctttga
    36541 ccagaaatca gtcgatccag tatatctttt tcataatgat aagttgcttc gaccatttgc
    36601 tggacattcc ctgtgttttt tgcatgtaag attgcttcga tcagtttgtt gcttttctct
    36661 aaactttcca caaattcttt ctcattctca tcttctttca acgtttcaat caaacgttgc
    36721 atctcttcat agttatgatt cagttcatcg aattctgtat aagcaagttt atgatcaaaa
    36781 aaaagtggga atgcaaagag cacgaccaac aatagaggaa ataaaaagtt tactttgttt
    36841 tttagtaaga gattgcgttc aaatgtaagt aaagacatag ttacacctca tataatttac
    36901 gataaatagc taaaatctct tccatatttg aggattctgt gacgatttct ccatttttta
    36961 gaaaacatgc tttagaacaa atcgattgga tattcgcgat agcgtgggaa gaaagaagaa
    37021 tcgtcttccc ctctttctgc cattgtctga tgaggctatt caagatatca atacttcctg
    37081 ggtctaatcc gttcaacggt tcatcgaata acatgacaga tgaatcactg atcaagtaag
    37141 cagcaagcaa gacatgttgt ttcattccta gagacatttt tccaatcggc aaagtaaggt
    37201 actcagtcat cttcaatcgt tggaaaacag tttggcattc tactttgctc tgccaatagt
    37261 atttgatcaa agtcaaataa tcaacagccg atagatagtc ggataactga ttcgttgatt
    37321 ctagaaaaaa gaaagaccgg cgataatctg tataagcagt aggagagatg ttgtgaaggg
    37381 acaattgatc ataagttgtt ggcagcagtt gggcaatgcc attcagcaaa gttgttttgc
    37441 caaatccgtt cggggcaatc aaagcagcta cttctcctga ttctactaaa agattcaatt
    37501 gtcgaatcac acgtttttta ccacgtttca tgttcatgtt tttgatcagt aatgtcatag
    37561 ataggcctcc aatataaaat ttaataacat aatagaatgg aaaacatcta aattaaatgc
    37621 taacaaaagt tattgtttgc atattataaa aaaattaaaa taacagtttt atttaaaatt
    37681 gaagttttct gataccgaaa tctttttatt ttgttgacat actcaaacct ttggcacaaa
    37741 gtttcgtgcc aaagtacgac tatttctctt tttagtaagc ggatacaatt agagtatcaa
    37801 aaggagagag ggagagacaa aatggaacgg acgattccca aaaacaacat ttcgctaaaa
    37861 gcgagggtac aaaaattagg cagtacttta tcaagcatgg tcatgccaaa tattggagca
    37921 ctgattgcct ggggtgtatt aacggctcta tttatcccag atggttattt accaaatgag
    37981 gctttcgcta ccatggtaag cccaatgcta acttatctga ttcctttatt gatcggttat
    38041 actggcggaa aagtaattgc tggtgaccgc ggatcagttg ttggggcaat tgcaacgatg
    38101 ggtgtgattg tcggaacaga tattccaatg atgctaggcg cgatgatcat gggacctctt
    38161 ggtggatatg ccatcaagaa atttgatcag ctattccaaa aacggatcaa atccggtttt
    38221 gaaatgctag tcaataattt ttcagcaggt cttatcggtt ttggattagc gttattaggt
    38281 ttttctgcga ttggtccagt tgtcgatgca ttgactcaag caatggcaaa aggtgtagag
    38341 atcattttat ctgcccattt gattccattg acaagtattt ttattgaacc agcaaaaatc
    38401 ttatttttga ataatgcaat caatcatggg attttgacac cattgggaac tgaacaagta
    38461 gcagaggctg gaaaatctat tctattcttg cttgaagcaa atcctggtcc aggacttggc
    38521 gtattacttg cttttacttt gtttggcaaa ggagcagcaa aatcttcagc accaggagcc
    38581 atgatcattc actttttagg cgggatccat gaaatctatt ttccttacgt tatgatgaaa
    38641 ccgttattat tcttatcagt tataggcgga ggaatgtcag gaagtttcat tttccaattg
    38701 atggatgctg gactaagagc gccagcttct ccagggtcga tcattgcgat tctagcgatg
    38761 acaccgttca attcttacct tcctgttatt ttgggcgtga ttgttgccgc agctgtttcg
    38821 tttggtattt ctgcagtaat tttgaaagcg gatgcgaaag atacaaatga agacttcgaa
    38881 aaatctgttc aagagacaaa ggcagctaag caagcttcca aaggtgccca aggtattcct
    38941 caaggagcta cattctctgg cgtacaaaaa atcatttttg cttgtgatgc aggaatggga
    39001 tcaagtgcga tgggcgcttc gatcttgaga aaaaaagtac aggaagcaga cttgcctcaa
    39061 acggtgacca atcaagcaat cagccaactg acagatgact cgaatacatt gatcgttact
    39121 caggtcgaac tacaagaaag agcaaaacaa aaagcaccga gcgcgcgttt tgtctcagta
    39181 gaaaactttt tgaattcacc tcgatatgat gaaatcattc aagaattaag cagcaagcag
    39241 aaaacatcag tttctgaaat tgagaatgag ccagcagaag agtttctttc agcaaacatc
    39301 aaacaaatca aagagattct acttgtccac gatgatcgtt ccggatcagc aacaatgggg
    39361 atgaccgtgc tcaaagatat attagacaag aaccatttaa ctatcccgtt aagaaaagtc
    39421 aatatccaag atttaaaaga tgaacctgat tcactgatcg tagccaaaga aagtttagcg
    39481 gaaaaagtac agatggctgc acctcaatcc attcatttat cagtcaacag catggtcaat
    39541 ccgcaaaaat acgaatcgat tgcacaatat ttgaaacaga cagcttaagg gaggaaaaca
    39601 gcatgcaaag tttgccattc gacatgatcg agctcaatcg atcttttaaa accaaagaag
    39661 aagcgatccg ctattgcggt cagatgcttg tagaagcagg gtgcgttgat gaggaatata
    39721 tcgatgccat ggtagataga gacaaaatgt tatctgttta tatggggaat tttatcgcga
    39781 ttcctcatgg aacagatgaa gcaaaaaaat atgtgaaaaa atccggcatc tgtgtcgttc
    39841 aagtaccaga tggtgtagat tttggtaccg aagaagaaga aaagattgcg actgttttgt
    39901 tcggtattgc aggagtcgga gatgagcatt tgcaacttgt acagcaaatc gcactttatt
    39961 gcagtgatat ggacaatgtt gtccagctag cagacgcgct cagcaaagaa gaagtaacgg
    40021 aaaatctagc aattgcttaa ggagagagaa aaatgagagc agtacatttt ggtgcaggaa
    40081 atatcggtcg aggatttatt ggagaggttt tggcagctaa tggatttgag attactttcg
    40141 tagatgtgaa tcacacgatc attgatgctt taaatgaaag aaaagaatac gaaatcgaat
    40201 tagcagatga atcaaaagaa aaaatcactg taaaaaatgt caaaggaatc aacaatcaaa
    40261 caaatccaga aaaagtaatc caagaaatcg ctgaagcaga tttagttact accgccattg
    40321 gcccgaatat cttacctttt attgctgagt tgattgcaaa aggtatccaa gaaagagaaa
    40381 aggaaggaag cacaacatct ttagatatca ttgcctgtga aaatatgatt ggcggttcag
    40441 catttctaga aaaagaagtg tataaatatc ttccggaaac atcatttact gacaaatata
    40501 tcggctttcc aaatgcagca gtcgatcgaa tcgtcccatt acaacaccat gaagatcctt
    40561 tattcgtcca agtggaacct ttcaaagaat gggtcattga cgatagccaa agaaaaaata
    40621 aaaaaatcca attaaaaggg gttttatatg tagatgatct agaaccatat atcgaacgca
    40681 aactatttag tgtcaatact ggtcatgcga ctgtcgctta tacaggtgcc ttgttaggtt
    40741 accagaccat tgatgaagca atgcaagatg cgttagttgt ggcacaatta aaatcagttt
    40801 tgcaggaaac aggaagtttg ctgattgcca aatgggggtt tgatgcagag cagcatcatg
    40861 cgtatattga aaaaatcatt catcgcttcc aaaacaaaaa tatctctgat gcgatcacac
    40921 gtgtcgctcg gacaccatta cgtaagttag gttatcaaga aagatttaca cgtccagtga
    40981 gagaactaca agaacataac ttgacttgcc cacatttgac ggcaacaatg gggattattt
    41041 ttaattacta tgatcctgaa gatgaacaaa gccgtcaatt gcatgaaatg aagatccatg
    41101 aaaacttgga acaattgatt caagaagtca caggaatcaa tgatccgaaa acaatcggga
    41161 atatcaagca aaacgtgaac cgttatgcca agcaagtcgc ttaaaatagg atacgaaaaa
    41221 ggggaatgtg acgaaatttt tgtcacattc ccctttttcg aataactagg ctgtttgtcc
    41281 ttcaagatag ggcagatcca attctttacc gtaacgttgt tctaaagcta ggcggatcaa
    41341 acgagctgcc aggttctcat ttcttgcaag gataggtcca tggaaataag aaccgaagac
    41401 atttcggtaa atgacacctt cagattggtc ttcaccatta tttccttgac cttgtaccac
    41461 tttccccaaa ggtcgttcac cttcacctaa gaaagtccga ccattgtggt tttcaaatcc
    41521 ataataagtt tcattgaatt cttcgttatg gataacgata tcaccgataa accggttatt
    41581 ttcctgactt aacgtgtagt gatccagtgc actgattcct tggatctttt ctccattagc
    41641 tcccatgtag tagtgaccta gcaattggta gccaccacaa atggctaaca tgacgccatc
    41701 attttcgata tagttgatca aagaatcttt ctttttctgg atgtcttttg aaatgattaa
    41761 ttgctcaaag tcttgaccac cgccaaagaa aaccaagtca tatttatcag catcaaaagg
    41821 ttcatgtata ctaacgatct ccgtatggca agaaaccccc attttttctg ctatatattt
    41881 caacatcaaa aggtttccgt tatcaccata tgtgttcatc aaatttccat aaaggtgggc
    41941 gatattgagt tcataattag ccacggttca ttcctccttt gataaatcct tgagcagcta
    42001 gttctttacg aagctgcaat acagcagtgt aagtagctaa aatatagaca tgctcagttg
    42061 gcaattcttt gattttttcg attacttctg gtaattcttt tgtttctgtc aatttatttt
    42121 ctggaatccc ggcaactttt agacgtaaag tcatatctgt atgacggtct cctccagaaa
    42181 taacagctgg gatatccata tcggcaaagt tttcaaaatt accatcccaa atccagctga
    42241 catcgattcc gtctgcgtaa ttggcattta ataaagccac taaagaaaat ggataaggag
    42301 ctagaccaat catatcgatt acttgattga tacctacagg atttttgaca agtactaatg
    42361 tacatttttt cccattgata ttgatgactt cttgtcggcc gaatactttc tcgtcatatc
    42421 ctaaaccttg tttgatttta tcaggatcga cattgaagta ctctgccact gcagtagctg
    42481 ctaaagcatt gtagacatta tacataccgc caacttcgat tccgtattca tgaccatcga
    42541 tcacgaaatc tgctgacaca ttgtccatcc gtaccatatc agttaatcga taatccaatt
    42601 caggacgttt gaagtcacaa tttggacagt aatatttccc taagtttgca taagtgatca
    42661 ttttataatg caagatgtga tgacaatttg gacataagac accgtctgta ttgtaatgag
    42721 ccatttgttc acgatcttct tcatggtcaa agccataata ttttctagga ttgactgttt
    42781 cgatagaatt gaagattggt gaatcaccgt tactgataat cgttgcattt ggtgactttg
    42841 cggcaccgtc tacaatcatt ttataggtcg tatagatttc tccataccga tccatttgat
    42901 cacggaaaat atttgtaaat aagaacagtt ctggtttcat atattctgtg actttgctca
    42961 aacttgcttc atctatttct aggacagcaa actttttctg tccttttttt ggttttgcct
    43021 gcaagaaagt cgatacaatt ccctgtacca tattcgcacc agtagggttg gttagaactt
    43081 catcgaattg ctggcgcaaa atattgactg ttaaagcggt agtcaatgtt ttaccgtttg
    43141 ttccggtgac aacgacgact tggtagtctt ttgccaaact atctagtata tgtggatcga
    43201 ctttcagcgc tagtttacct ggtaaacttg atccgccttt aaaaaaagtt tttaatgccc
    43261 attgcgacgt ttttccaact gcaatcgcaa aatgactgcg tattcccatt atgtaacctc
    43321 caaaaacgat ttgctgacga ataaattcaa ctaatcatac catatgttga aaaaaaggtt
    43381 aaattgcttt tcttattctt aagaaaaggt tacggctgaa agtgcatgaa atcggacaaa
    43441 atcgcatgat ttaacaaaaa aacagcttga aacaaaatga ttgtttcaag ctgctgtgat
    43501 tttattattc cctcttacct atattcccat tttaaccgat aataattttc tcgcttggat
    43561 attcatagcc tagatctcgt gtttcttttg gcacgaataa catcaatgta tatagcattc
    43621 cgatccgccc aataaacatc agtgtagcaa tcgtgatttt gcccacaaca gacaagtctc
    43681 cggtaatccc taaagataaa ccagttgttc caaacgcaga agttacttcg atgatgatcg
    43741 agatcagcgt ttgttcttct gtagcagata aaaaaacgat acaaaagaaa cacataccaa
    43801 gagaaagcat aaagacaacg actgattttc gcacatcatc ttgatcgatt ctccgtccaa
    43861 aaatattgat gttatcttca cttttgagga aggaatatag atacaaaccg atgattgcta
    43921 cagtagttgt tcgaatcccg ccgcctacag aactaggact gcatccgata aacatcagta
    43981 aagaaaaaat gatcagtgtt gtgatttgaa aatcacccaa atcgtgaatc tgcagaccag
    44041 cattacgagt cgtaatcgaa taaaacatcg aattgatcca tttgacactc atattggaat
    44101 cttggaataa atggtccttt tccaatagat aaatcaagac agttcccgaa acaaacaaga
    44161 taacaaaagc taacacagct aatttggtaa acaaggaaaa acgaaacgga agcttcgcat
    44221 tggaacgttt gtataatagc cattcccggc actccatcaa tacaggaaaa ccaattcctc
    44281 cgacaaaaat caaaaacatg atcaggatca agaaaaaata gtcatgtgca aagggcttga
    44341 tggaatcccc agtgacgtca aacccagagt tcgtgacagc cgatatcgcc tgataaaaac
    44401 cgtaaaaaac ggcatcacgc caacgatcaa aatagcctct gtaataaaaa taaatagaaa
    44461 agaatgtccc aaaaagtaat tgaaaccaga ttaaaatagc aaaagtgatc cgaatcagtc
    44521 ggacgatacc agacaaacgc ggttgattca tatccgtcat gatcagctgt cgctgtttca
    44581 gtgtgatccg tcgtttagaa aaaatgacaa aagcagtaga aatcatcatg atccccaaac
    44641 caccaacttg aaataatatt tccagcagta taataccgtt atcattgaac acagaattga
    44701 tatcaaaagt cgatagtcca gtcacactca ctgtactgat tgccatgaac agcatgtcca
    44761 caaaagaaac atggctatct ggttcgcgaa aaatcggcat ataaaataga acgagtgata
    44821 agaccgtcat caatatataa taaaaaacga tgatttggat cgatgagaaa tgattggcag
    44881 caaacttctg tcctcgccgc tgcagccttc gaaagaataa atcaacatgc acagtactat
    44941 cctcctgaca atagtcgttt ctaccagtat acaaggggaa ttaggaatac tcaaagaaat
    45001 tatcaaacag ataacaatac agctttcgat taaaatagaa atggcttata ataaggaaaa
    45061 tcctagaaaa aagaaggagg aggaataaca aattgattaa tgctcaacaa atcgtagaaa
    45121 aaatgaaaaa tcaaaaaata aattatgata cagttctgaa aaaaatgatc atgcaatggg
    45181 agaaagaaga agtaaagcct aagatattaa tacatacatg ctgtgctcct tgcagtacgc
    45241 acacattgga attcatgtct caatatgcag agatcacgtt atttttttca aattcgaata
    45301 ttcatcctaa aagtgagtat ttgcgaagat tacatgaaca aaaacgattt gtagaccaat
    45361 ttaatgagaa aactgaccat catgttgcgc taatcgttga tgaataccgt ccaaatgaat
    45421 tcaccaaaat ggttttaaca aaccatcttg aaaacgaaaa agaaggtggt gctcgctgca
    45481 gtgcatgctt caatatgcga ttagatcttg ttgcgcaaaa agcgcaagaa ctaggatacg
    45541 attactttgg aagtgccctg accatctcgc caaagaaaaa tgcagctttg atcaatcaga
    45601 tcggaatgga tatccaaaag atttacggga cacaatactt gccaagtgat ttcaagaaaa
    45661 acaaaggcta tgaacgctct ttgcaaatgt gcaaagagta tgatatttat cgccaatgtt
    45721 attgtggttg cgtgttcgct gccaagcagc aaggatgcga tctgaaagaa atcaatcggg
    45781 aagcaaaagc ttacttaaaa acatcaaccg tagtttttga ggaaaattaa agaaatcttt
    45841 cggaaaaacg taagaaaaaa ataaagaatt tatggtagaa ataaggtaga ctgtataatt
    45901 tgtccgtagt aaagtaagga aacaagaata aatttcaatg aaggaagaga taagatgcga
    45961 aaagtagcac ctattttgaa gccaatcgct gtattaacaa tctcgacgat tgtcgcgact
    46021 actacaggta ttgtgaatag ttccagctac cgtgcaccaa gttcaagttc tagtactgta
    46081 gaagaaacaa caccaactac ttcagtctcg acatctgaac caacaacaac ggaaagtttg
    46141 aatgaaacca ctacgacaag tacatgggac gatacaacaa ctagttcgtc gtatgatgag
    46201 acatatacaa ctgacagctc aagcgaaaca agtaatacgg taccagatgg cactccatcg
    46261 gattcaacca atccaagtac aagtccggat acaagtgcta gctccgaaac gataccaagc
    46321 agtacgactg tagatgataa cacgcaaaat gatgatgcag cacgtacagc acagcaaaac
    46381 caagattcag gaaataccca aacgtataac cagcaagcag gtgattctca atgacatttt
    46441 tactagacct tatcaacggg gtgcataaat tccttggcta tttagacatc agtcctaaat
    46501 atcttaaccg tggatatacg attttaagtg tgattcccac gctttattta ttgagaattg
    46561 tttatggact ttggcaaaac caaaactatc ttcaattttt cttatatgga cttgcatttc
    46621 tagtgcttgt ttattttact gttttaaatg tattttatta ctttttagat aaaaacacga
    46681 aagccgatgt cactcagctt tttgtgaagt atttacctga tgaagcgttc aacatccaga
    46741 cagaagaatc gatagataat ggaaattcga tcgataaagt gaatacacaa gaggtcatgg
    46801 tttcttatga cgaagattat caattgaaat tagccgagaa tatgcggtat ttgattggaa
    46861 atggtgagat caaaacaaat gaactcggtc gcatggatgg atttctggtc gatagaaata
    46921 cattgtatcc ttattactat gtcaagaaaa caggagagaa agagtatgtt ctaaatattg
    46981 gacgaagcta ctcggacatg gaaaaagtag ggaccattcg acttgactca acagaagaca
    47041 cattggaacc tgtcggattg ttcatcgtcg gcggtgattt tgtcaaagac gggttacgct
    47101 atcatgagcc ttatcgtctg aaattgattg caaaaaaggc acagtcgcag gaagaaccag
    47161 tggtttatag tcgtagccaa agaagaagac attcataatg tgatgaaacg ccgtgtatca
    47221 gtggttgcga taaaaacgtt tattcggaat aagggggatg tgacacgtct tttgtcacgt
    47281 tcctttttta tataacgaga aagataagac gaacacatca cttattcttt cctttcattt
    47341 ataaacattg tagaataaag aaagtacaac gaagtattgt gaaaaaacgt aataaataac
    47401 gaggaaaata caagtaggag gtaagctgtg aaagaatttt tgaaaaattg ggggattttg
    47461 atccttgtat tagcggctat tttattagct agagtgtacg tgtttacacc agttactgta
    47521 aacgggcatt cgatggaccc tacattaagt gatggacaac gattgatttc atctaagatc
    47581 tcaaactatg aacgaatgga tattattact acaaaggaac cgggggatga agaacggatg
    47641 attgttaaac ggatcatcgg tatgcctgga gatacagtca agatggaaaa tgaccagttg
    47701 accattaatg gcaaaaagta tgatgaacct tatctggatg agttcaaaaa agaattttca
    47761 gaagataagt tacaaggaga atatgcttat agctcaggat tccaagcaca agcagaaagt
    47821 tcatccacct ttacgagtga ctttgaatat actgtgccaa aaggaaagta tcttgttcta
    47881 ggcgataacc gcttgatttc aaaagacagc cgaatgtttg ggttagtcga caaagatatg
    47941 atccaaggaa aagttgtttt ccgttattgg cctctttcag aaattaaaat catgtgatga
    48001 aaaaataaaa agaagctgtg acatgttttt tgtcacagct tctttttccg aaaattcctt
    48061 cttatttctt ggagttgctc actttttttg caatctcatt ttaagctttt tggctgatgg
    48121 ataagaaaag cgaagcgttt ttgttcataa atagcttttc cccacgatgg aaggtatgac
    48181 tagtagcaga gacaaagccc gttttttcca atcgattatt caattcttct tggctaaagc
    48241 cattatggac tttcggatga ctaatttgtt cattcttgtc aaagtctacg atgatcaatt
    48301 gaccgccagg agctaaaatt tcataaagtt ttgtaagaat attctccgta tcaggaatgt
    48361 gtaaaagtac aagtgataga aggatgagat tagcttggac agcaggaaat tcgacagaag
    48421 catcagcgtg gatcgttcgg acatttttca gatctgctgc ttggatcttt tcttctgcca
    48481 ttttcagcat agtttctgaa gcatcggcaa tgatcagttc tttgaatctt tccgcaagcg
    48541 gtaaactaac aagcccagtt ccgcctccgt aatccagcag gactttttgg tggctgtcgc
    48601 gcggtagata agtagttagt tcttcgcgaa taatcttggc taatgcttga cggtcagggg
    48661 aatcataatg gttagcgatg gaatcaaaaa tattttttgt catcaaatag ctccttacgt
    48721 aaaactttga aattttaact tattatagca cgtgaaaaga aagaaagcat gctatactca
    48781 attcaaaaag ggagaatcta gaaagaatat gccgatatcg tttgtaaaag atagagagga
    48841 aaaaggaaag tgtgtccgag aaatattact agacttgcct gagtggtttg ggcttcctga
    48901 atcaacggaa aaatatatcg aggaatcaag taaactacct ttatggtgtg aaaaaagaaa
    48961 agaagaatat ttaggcttta tcactctttc acagacaagt gaagatactg ccgaaatcta
    49021 ttctattgta tgggaataaa aaaagactat catcaccaag gattaggaac aaaacttttt
    49081 aaagaagcag aaggttacgc agcaaaacat tacaaatatt tgcaagtgaa gactgtagac
    49141 gaggggcact actctatata tgatcaaact atttgtttct acgaatcatt aggattttca
    49201 aggttggaag tttttccaaa tttatgggat gaatggaacc catgtttagt tcttgtcaaa
    49261 aaattggaac aaaagtgagc aatagattta atactaatct gtgcttgatg aaaattctca
    49321 ctgtaaaaaa tgctgaaaat caagtataat accataggaa caaactggaa ggtgaaaaag
    49381 agcatggcac aactattttt taagtatggc gcaatgaata gcggcaagac gatcgagatc
    49441 ctaaaagtcg cacataatta tgaagaacaa aataagccag ttgtcttaat gacaagcggt
    49501 attgatacaa gagatggttt aggcgttgtt tcaagccgaa tcggactgaa acgtaatgcg
    49561 atgccgatat ttgaagaaac agacgtattt gacgtaattc agaaaatgga aagcaaaccc
    49621 tattgtgtgc tgatcgatga agcgcaattc ttaaaaaaga aacatgtcat tgcttttacg
    49681 agaattgtgg acgaattgga tattccggtt atggcatttg gtttgaaaaa tgattttcga
    49741 aacgagttat ttgaaggatc aaaatattta ttgctttatg cagacaaaat cgaagaaatg
    49801 aaaacaatct gttggttctg ccacaaaaaa gcaatcatga atttacatta tattgatgga
    49861 aaccctgttt atgaaggcga tcaagtgcaa attggcggaa atgaagctta ttaccccgtt
    49921 tgtcgaaaac attactttca cccatcaatg attacagaag gagaataagc atgtacgatc
    49981 aattacaagc aattgaagac cgttatgaag agcttggaga acttttaagt gacccagaag
    50041 tgatttctga tacaaagcgt tttatgcagc tttcaaaaga agaagcaaac acgcgcgaaa
    50101 cagtcgaagt ttatcgtcgt tataaagaag tagtacaagg aatcaccgat acagaagaac
    50161 tgcttggtga aaaactggat gatgaaatgg ctgaactagc aaaagaagaa ttatctgaat
    50221 taaaaaaaga aaaagaagtc ttagaagaaa aaatcaagat cttgctttta ccaaaagatc
    50281 caaatgacga taaaaatatc atcatggaaa tccgtggagc agcaggcgga gacgaagcag
    50341 cattattcgc tggcgatctt ttcaatatgt accaaaagta tgctgaagca caaggctgga
    50401 aaacagaagt gatggaagct aacgttaccg gaatcggtgg atataaagaa gtgatcatga
    50461 tgatcaccgg agacaacgta tattcgaaat taaaatatga aagtggtgcg caccgcgtcc
    50521 agcgtgttcc ttcaactgaa tcgcaagggc gtatccatac ttccaccgca acagttgtcg
    50581 tgatgccaga agctgaagaa gtagagatcg atattgaaga caaagatatc cgtacagata
    50641 tctatcatgc ttcaggtgct ggcggacagc acgtcaataa aacagcatct gctgtacgtt
    50701 tgactcactt accaacaggg atcgttgttg ctatgcaaga tgaacgttct caaatcaaga
    50761 accgtgaaaa agcaatgaaa atcttgcgtg ctcgtgtgta cgataagatc cagcaagaag
    50821 cacaaagcga gtatgatgcc aatcgaaaat cagcagtcgg aactggggat cgttcagaac
    50881 ggatccggac gtataatttc ccgcaaaatc gtgtgactga tcatcgtatc ggattaacta
    50941 tccaaaaatt agatcagatt ttagcaggga aattagatga gatcgttgat gcattgatca
    51001 tgtatgatca aacatcaaaa ctggaagcta tgcaaaatgg gtaaaatgac ctatcgagaa
    51061 gtcctgtcac gggcttcttc ttttttagag gatcatggga aagaagcata tagtatccaa
    51121 tttttatttt tggaacgaaa acaatggaaa aaacttgatt ggctccttca tatgaacgaa
    51181 gagatttcag aagaagaaca gcggttgatc gaaaccgatc tgcgacttct gcttgcagat
    51241 catccacctc agtatttgct aggatatgca gatttttatg atcatcggtt aaaggtaact
    51301 gaagcaacac tgatccctcg accagaaaca gaggagttag tcgaatggtg tttagacgag
    51361 acgcctggtg taccgttaga agtgatagat attgggacag ggactggagc aattgctatt
    51421 agtttgaaag cagcaagaaa aaattggcat atatctgctg tggatctttc agaagaagca
    51481 ttagaagtag cgaaagaaaa tgctcagaaa gaaggaacga aaatcagttt ttatcatgga
    51541 gatacattag gaccagtaat ggatcagcaa tttgatgtga ttatttctaa tccgccgtac
    51601 atcagtcgga atgaatggga actgatggat gaaagtgtcc ggtcattcga acctaaaatg
    51661 gccctttttg ctgagaacga tggattagct atttatgaaa aaatagcagt tgaagcacca
    51721 agcgtactaa aatcaaatgg aaagattttt ttagaaatcg gatttcgcca aggtgaagca
    51781 gtaaaaaaaa tatttcagca agcatttcca gataaaaaag tggcgataaa aaaggatctt
    51841 tttggcaatg aacgaatgat ccaagtttat ggttgaagtt gtggatgaaa aattttttat
    51901 ttgttttttt attataaaaa aacacttatt tgacaaggat gtaaacgact ttttggttta
    51961 tcaacaagtt tatccactat tgacaaatag attatccaca aacagtggaa aacttgagga
    52021 taaactcaga caaaataaga atgtatgaaa aaggggttgt ggataaactg tggaaacgaa
    52081 acgatatgat gaaacagaat tgcaagaggc agccgaagca ctgaaagaag gagaacttgt
    52141 tgcttttcct accgaaacag tatacggatt gggcgccaac gctcttttac caaacgctgt
    52201 caagaaagtg ttttctgtaa aagggagacc gcaagataat ccattaatcg tccatgtcgc
    52261 ctcttttgaa caagtaaaag agtatgtgga caactttcat ccggcagcag agaagattgt
    52321 gaagaacttc tggcctggac cattaacttt gatatttaag atcaaaaaag acactttgcc
    52381 ctcagttgtg acgggaggat tatccacagc tgcatttcgt ataccggata acaaaaaaac
    52441 actagaagta atcgaacttt ccggtgttcc gcttgtcggt ccaagtgcaa atacgtcggg
    52501 aaaaccaagt ccaacgactg cagatcatgt atatcatgat ctacaaggaa aaattaccgg
    52561 gattattgat gatggagcta cgcgaattgg tgtagaatcc actgttcttg atttaagtga
    52621 tccaacagcc atgcctatga ttttacgacc aggtgcagta acgaaagaac aaatcgaagc
    52681 agtcatcgaa tctcctgttg ctattgatca gcatcttgta aaagaaaatg aaacaccaaa
    52741 agcccctggg atgaaataca aacactactc acccgataca cgtgttctga tggtcagaga
    52801 tggcgattgg tcaactgcag ttcaatgggc aaaaaataag aagatccgtg caggtgtcat
    52861 agcaagcccc gaaattgcgg atcaagtgcg gacagatact gcagctgttt atatgtataa
    52921 cgacaactca gtagaagcgg cggctaaagg attgtttgca ggtctaagag gattagatga
    52981 accaacactt ggattagatt tgatttttgt acaagtgtat ccagaaactg gattggggaa
    53041 tgcttacatg aatcgcttga aaaaagcagc aggacaaaat tattttgaaa aatgatcaat
    53101 tggttattgt cgcattcttt tagtatgata caatgctatc aagtaaatct tctatcagtg
    53161 aacgaaaaac taagcgtaac taatcgtaat agaagagata cgaatatttg gcaatttgtt
    53221 tttgatggga aaaataaaat tgtaggaaaa taaaaaggag ctgttgtttg tggtagatta
    53281 caaaacgttt gaccctgatc tttgggctgc gatcgccaaa gaagaagaaa gacaagaaca
    53341 taatttggag ctgattgcct ctgaaaactt tgtctccgaa gcagtgatgg ctgcacaagg
    53401 aagtatcttg acaaataaat acgcagaagg ttatccaggg catcgctatt atggtgggtg
    53461 tgaatttgtc gatatcgttg aaaatttagc gattgaccgt gcaaaagaat tattcggagc
    53521 gaaatttgcg aacgtccagc ctcactccgg ctctcaagcc aacacggctg cttatctcgc
    53581 tttggtagaa ccaggggaca cgatcttagg aatggatctt tccgcaggcg gacatctaac
    53641 acatggatca ccagtcaact tcagtggcaa aacctatcac tttgttgctt acggtgttga
    53701 tccgacaaca gaagttattg attataatgt ggtacgtatt ttagctagaa aacatcaacc
    53761 aaaattgatc gtagcaggtg ccagtgctta cggacgaacg atcgactttg cgaaattcag
    53821 agaaatcgca gatgaagtag gcgcaaaact gatggtagat atggcacata tcgcaggatt
    53881 agtagcagca ggacttcatc ctaatccagt tccttatgca gatatcacaa cgactaccac
    53941 tcataaaaca ttgcgtggcc ctcgaggcgg catgattttg acaaacgatg aagcattagc
    54001 aaaaaaaata aatagtgcgg tatttcctgg tattcaaggc ggccctctag agcatgtgat
    54061 tgcgggtaaa gcagtagcat tcaaagaagc attagatcct gcatttaaag aatatagtga
    54121 acaaatcatc gccaatgcaa aagcaatggt caaagttttc aatcaagcaa ttggaaccag
    54181 agtcatttca ggggcgacag ataatcatct gatgttgatc gatgttcgcg agttaggtat
    54241 aaatggaaaa gaagcagaga gcattttgga cagtgtgaat atcactgtaa ataaaaactc
    54301 gattcctttt gaaacactaa gtccattcaa aacaagcggt attcgaatcg gcacgccagc
    54361 aatcactact cgcgggttca aagaagaaga tgctgtaaaa gtggctgaac tagttgtcaa
    54421 agcattacaa gcaaaagatg ataacgctca actagacgaa gtcaaaacag gtgtacgcga
    54481 attaactgaa aaatttccgt tgcataaaaa ataaaaattt aaattccatt agtaaacaga
    54541 aaagttcaat gttgggcttt tctgtttatt ttttgggaaa acggattctt tgacaatatc
    54601 ggtgatgctc tctagtatat gaggtgtaat tttagtcttc acactaaatc tcagggagga
    54661 aaagtcatga ctgatcgtat ctcgttagaa aaggaagcag tagcattcag tgatgcaaat
    54721 gcgcctcatc cgcgtatcta tgaactagca ccggaagaag gaagagtttt actagaagaa
    54781 gtacaagaat cacctgtgaa caaatattcc gtcgatattg aagacttgat ggtggatact
    54841 ggcgaatggg gaaaactcaa tattcgtctc gtacgaccag aagggaatat ggataaatta
    54901 ccagttattt tttatattca tggagcaggc tgggtttttg gcagtgctca aacccatgat
    54961 aaattgatca gagaacttgc ggttcgtacg aattccgtcg ttgtctttcc agaatatagt
    55021 cgatcaccag aagcaaaata tccaacagca attgaacaaa attatgctgt gttgcaacag
    55081 ctgaataaat tagccaagtc aaaaaatctg gatctcgaga atctaatcgt tgcaggagat
    55141 tcagtaggtg gtaacatggc tacagtcatg acgatcttaa caaaagaacg aaagggcctc
    55201 ccaatatcca agcaattgct gtattatcca gtcacaaacg ctgattttga tacagattcc
    55261 tatcatgaat tcggagaaaa ctattttttg acaagagaag gaatgatgtg gttttgggac
    55321 caatatacga cgaatgaaga agagcgtgct gaaatcacag cttcgccttt gagagcaaat
    55381 ataaatgaac taaatgatct tccttcagca atgattttaa cgggagaagc ggatgtatta
    55441 agagatgaag gtgaggctta tgcgcgcaaa cttcgtgatg caggggttcc tgttacacaa
    55501 gtacgcttcc aaggaatcat ccatgatttt gtcatggtaa atgctatgga tcagacaaag
    55561 gcaacgcgtg cagcaatggc attatctact tcttggatca agaaaaaaga ataagctttg
    55621 cttgtccgca ggaaaacttc ttttcaaaaa cggattttct taggaaaaat ggcgagtttg
    55681 actagtcaat cacttgattt tgttttacaa ttaaaaagaa acgacagaac aaaggagagc
    55741 ttaaaaaatg ggcaaatttc aagtgatcga tcatccattg attcaacaca aactaactat
    55801 tattcgggac aaaaactgcg gaacaaaggt ttttcgtgaa gtagtcgatg aaatcgctat
    55861 gttgatggca tatgaagtat cacgggatat gccattggaa gacatcgtga tcgaaacacc
    55921 gatcactgaa acaacacaaa aaacgttgtc tggtaaaaaa gtagcgatta ttccaatctt
    55981 gcgcgcaggt attggaatgg ttgacggtat cttggaattg attccagctg ctaaagtggg
    56041 acatatcggt ctttatcgag atgaagaaac attggaacca catgaatatt tcgtcaaact
    56101 tccagaagac attgatgcac gtcagttgtt tgtagtagat ccaatgttgg caacaggcgg
    56161 atcagcaatc atggcaatcg atgcattgaa agaacgtggc gcgtcgaaca ttaaatttgt
    56221 ttgtttggta gcagcacctg agggagtaaa agctttgcaa gaagcgcatc ctgatattga
    56281 catctataca gcttctctag atgaaaaatt gaatgaacat ggttatatcg ttcctggact
    56341 aggggatgcg ggtgaccgtt tgtttggtac aaaataagta aatacgaaaa agggtgtgac
    56401 acaacttttg tcacatcctt ttttggtcat ttttaacgtt tgatgcagaa cagctttttc
    56461 acagctttcg tttgcatata aaaaaaccat cccaaaaatg cagagatggt caaattcaaa
    56521 tcttttttag ttctttgtag ttaacgatta ctacaccctt actttgcgtc atcaagttcg
    56581 cattttctgc agtttcatca atctcaacaa cagcggaatt tttcaattgc tttgaaatcg
    56641 ttccagtttg cgacttgccg tgaattgtga acattacgtg agtattgatc ttgaattttt
    56701 caccaatttc cgatggtacg atagttgtat caaattttcc aaaagttgcc attttcataa
    56761 aacctgcctt tcacatcaat tataccacgt tttatgaaaa atatcatgaa attaaactaa
    56821 ttttccatcg gactctttac aatcagccaa atagagtata taatttcttt tggataaggc
    56881 cagcttagct cagttggtag agcaacgcac tcgtaacgcg taggtcgtag gttcgatccc
    56941 tatagctggc attaaaagag aagacagata tatcaaggat ttttaatcca ttgatacatc
    57001 tgtctttttg tttaaaggaa ataaagtatg ctttcttagc aatagagcat gtattatttc
    57061 tataaaaatt taggagaata aaacgactag caatatagct gtgaaatcaa atatcacatg
    57121 aactgcccaa cttgtaatga tcgagttgct tttaatggca gcttggttga ataacaatct
    57181 tgccacccct tgtatcaaaa ggatgtgtgc tacagtgtgt aaaatatttc cattatcata
    57241 agtactgtaa tggataaatc caaagatgat tgctgacaag aaactagcga caggaatgct
    57301 gaacttacta gaaaaaatag cgagaaaata cactgagaac aattcttctc caagtatcat
    57361 gataggcaaa ataataaata ataacgggct gaaagcctca tttattggat tgcctgttaa
    57421 accccatttc aaaatttgtt gcaaaaatac cgagactgcg gcactgatta ctacattgat
    57481 aaaaaaatat ttggctatat ttttgacagg agcgacaggt ttcgagaaca tttttttcgt
    57541 atctttttta ccaaaaacaa gtgtaaatgc tataaatcca gtgatagtta aagaaaaaat
    57601 gctaataatg gatggcgcaa atagcaaccc taacaaaccg agtacaacga caatagtgcc
    57661 caatatccct tttttaagtt ctattttttt tcctaaaatg ttattcatga taataactcc
    57721 tactattcag tttttgaaga tagagtcagt atacatgaag agttaaaata ggcaatgttt
    57781 ttttatggat atttgt
//
LOCUS       DAZUMK010000005        53713 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000005 DAZUMK010000000
VERSION     DAZUMK010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.005"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            29..1660
                     /locus_tag="ACG808_000316"
     CDS             29..1660
                     /locus_tag="ACG808_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFS5045976.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(1722..2363)
                     /locus_tag="ACG808_000317"
     CDS             complement(1722..2363)
                     /locus_tag="ACG808_000317"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFS5045977.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    2549..2625
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            2645..2953
                     /gene="rplU"
                     /locus_tag="ACG808_000318"
     CDS             2645..2953
                     /gene="rplU"
                     /locus_tag="ACG808_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFS5045978.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            2980..3324
                     /locus_tag="ACG808_000319"
     CDS             2980..3324
                     /locus_tag="ACG808_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFS5045979.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            3337..3630
                     /gene="rpmA"
                     /locus_tag="ACG808_000320"
     CDS             3337..3630
                     /gene="rpmA"
                     /locus_tag="ACG808_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFS5045980.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            4064..6871
                     /locus_tag="ACG808_000321"
     CDS             4064..6871
                     /locus_tag="ACG808_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS5045981.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            7062..7553
                     /locus_tag="ACG808_000322"
     CDS             7062..7553
                     /locus_tag="ACG808_000322"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFS5045982.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            7656..8645
                     /locus_tag="ACG808_000323"
     CDS             7656..8645
                     /locus_tag="ACG808_000323"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFS5045983.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            8678..9481
                     /locus_tag="ACG808_000324"
     CDS             8678..9481
                     /locus_tag="ACG808_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFS5045984.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            9502..10416
                     /locus_tag="ACG808_000325"
     CDS             9502..10416
                     /locus_tag="ACG808_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS5045985.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVERWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            10499..10873
                     /locus_tag="ACG808_000326"
     CDS             10499..10873
                     /locus_tag="ACG808_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFS5045986.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            11012..11257
                     /locus_tag="ACG808_000327"
     CDS             11012..11257
                     /locus_tag="ACG808_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS5045987.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            11429..12493
                     /locus_tag="ACG808_000328"
     CDS             11429..12493
                     /locus_tag="ACG808_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFS5045988.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            12739..13164
                     /locus_tag="ACG808_000329"
     CDS             12739..13164
                     /locus_tag="ACG808_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS5045989.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            13157..13606
                     /gene="nusB"
                     /locus_tag="ACG808_000330"
     CDS             13157..13606
                     /gene="nusB"
                     /locus_tag="ACG808_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFS5045990.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            13703..14557
                     /locus_tag="ACG808_000331"
     CDS             13703..14557
                     /locus_tag="ACG808_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFS5045991.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            14558..15895
                     /gene="xseA"
                     /locus_tag="ACG808_000332"
     CDS             14558..15895
                     /gene="xseA"
                     /locus_tag="ACG808_000332"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFS5045992.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            15900..16124
                     /locus_tag="ACG808_000333"
     CDS             15900..16124
                     /locus_tag="ACG808_000333"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFS5045993.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            16126..17016
                     /locus_tag="ACG808_000334"
     CDS             16126..17016
                     /locus_tag="ACG808_000334"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFS5045994.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            17025..17843
                     /locus_tag="ACG808_000335"
     CDS             17025..17843
                     /locus_tag="ACG808_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFS5045995.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            17950..18399
                     /locus_tag="ACG808_000336"
     CDS             17950..18399
                     /locus_tag="ACG808_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFS5045996.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            18417..20099
                     /gene="recN"
                     /locus_tag="ACG808_000337"
     CDS             18417..20099
                     /gene="recN"
                     /locus_tag="ACG808_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFS5045997.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(20154..20270)
                     /locus_tag="ACG808_000338"
     CDS             complement(20154..20270)
                     /locus_tag="ACG808_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFS5045998.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(20343..21299)
                     /locus_tag="ACG808_000339"
     CDS             complement(20343..21299)
                     /locus_tag="ACG808_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFS5045999.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            21470..21838
                     /locus_tag="ACG808_000340"
     CDS             21470..21838
                     /locus_tag="ACG808_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFS5046000.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            22008..22439
                     /gene="mraZ"
                     /locus_tag="ACG808_000341"
     CDS             22008..22439
                     /gene="mraZ"
                     /locus_tag="ACG808_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFS5046001.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            22460..23419
                     /gene="rsmH"
                     /locus_tag="ACG808_000342"
     CDS             22460..23419
                     /gene="rsmH"
                     /locus_tag="ACG808_000342"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFS5046002.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            23424..23825
                     /gene="ftsL"
                     /locus_tag="ACG808_000343"
     CDS             23424..23825
                     /gene="ftsL"
                     /locus_tag="ACG808_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFS5046003.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            23826..26018
                     /locus_tag="ACG808_000344"
     CDS             23826..26018
                     /locus_tag="ACG808_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFS5046004.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGMFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            26044..27006
                     /gene="mraY"
                     /locus_tag="ACG808_000345"
     CDS             26044..27006
                     /gene="mraY"
                     /locus_tag="ACG808_000345"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFS5046005.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            27020..28381
                     /gene="murD"
                     /locus_tag="ACG808_000346"
     CDS             27020..28381
                     /gene="murD"
                     /locus_tag="ACG808_000346"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFS5046006.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYRHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            28407..29495
                     /gene="murG"
                     /locus_tag="ACG808_000347"
     CDS             28407..29495
                     /gene="murG"
                     /locus_tag="ACG808_000347"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFS5046007.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            29510..30730
                     /locus_tag="ACG808_000348"
     CDS             29510..30730
                     /locus_tag="ACG808_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFS5046008.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            30886..32211
                     /gene="ftsA"
                     /locus_tag="ACG808_000349"
     CDS             30886..32211
                     /gene="ftsA"
                     /locus_tag="ACG808_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFS5046009.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            32234..33475
                     /gene="ftsZ"
                     /locus_tag="ACG808_000350"
     CDS             32234..33475
                     /gene="ftsZ"
                     /locus_tag="ACG808_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFS5046010.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            33492..34169
                     /locus_tag="ACG808_000351"
     CDS             33492..34169
                     /locus_tag="ACG808_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFS5046011.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            34183..34782
                     /locus_tag="ACG808_000352"
     CDS             34183..34782
                     /locus_tag="ACG808_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFS5046012.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            34863..35084
                     /locus_tag="ACG808_000353"
     CDS             34863..35084
                     /locus_tag="ACG808_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFS5046013.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            35133..35915
                     /locus_tag="ACG808_000354"
     CDS             35133..35915
                     /locus_tag="ACG808_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFS5046014.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            35936..36643
                     /locus_tag="ACG808_000355"
     CDS             35936..36643
                     /locus_tag="ACG808_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFS5046015.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            36905..39703
                     /gene="ileS"
                     /locus_tag="ACG808_000356"
     CDS             36905..39703
                     /gene="ileS"
                     /locus_tag="ACG808_000356"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFS5046016.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(39752..41278)
                     /gene="zwf"
                     /locus_tag="ACG808_000357"
     CDS             complement(39752..41278)
                     /gene="zwf"
                     /locus_tag="ACG808_000357"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFS5046017.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(41293..41940)
                     /locus_tag="ACG808_000358"
     CDS             complement(41293..41940)
                     /locus_tag="ACG808_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFS5046018.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(42124..42453)
                     /locus_tag="ACG808_000359"
     CDS             complement(42124..42453)
                     /locus_tag="ACG808_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFS5046019.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            42630..43358
                     /gene="sfsA"
                     /locus_tag="ACG808_000360"
     CDS             42630..43358
                     /gene="sfsA"
                     /locus_tag="ACG808_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFS5046020.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            43374..44387
                     /locus_tag="ACG808_000361"
     CDS             43374..44387
                     /locus_tag="ACG808_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS5046021.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            44387..45664
                     /locus_tag="ACG808_000362"
     CDS             44387..45664
                     /locus_tag="ACG808_000362"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS5046022.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            45727..48429
                     /locus_tag="ACG808_000363"
     CDS             45727..48429
                     /locus_tag="ACG808_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS5046023.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENRIKK"
     gene            48581..48898
                     /locus_tag="ACG808_000364"
     CDS             48581..48898
                     /locus_tag="ACG808_000364"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5046024.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            48928..49248
                     /locus_tag="ACG808_000365"
     CDS             48928..49248
                     /locus_tag="ACG808_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS5046025.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            49356..50816
                     /locus_tag="ACG808_000366"
     CDS             49356..50816
                     /locus_tag="ACG808_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS5046026.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            50884..51105
                     /locus_tag="ACG808_000367"
     CDS             50884..51105
                     /locus_tag="ACG808_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046027.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            51136..51318
                     /locus_tag="ACG808_000368"
     CDS             51136..51318
                     /locus_tag="ACG808_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFS5046028.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            51318..51731
                     /locus_tag="ACG808_000369"
     CDS             51318..51731
                     /locus_tag="ACG808_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFS5046029.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            51854..53035
                     /locus_tag="ACG808_000370"
     CDS             51854..53035
                     /locus_tag="ACG808_000370"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5046030.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(53106..53270)
                     /locus_tag="ACG808_000371"
     CDS             complement(53106..53270)
                     /locus_tag="ACG808_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046031.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            53424..53497
                     /locus_tag="ACG808_000372"
     tRNA            53424..53497
                     /locus_tag="ACG808_000372"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:53458..53460,aa:Arg,seq:cct)
BASE COUNT        17729 a         9119 c        11618 g        15247 t
ORIGIN      
        1 aaaatttcat gaaaaaggag ttagataggt gttcaaaaat tatgtttcct cactaaaaaa
       61 agaattttca ggttatgatc tttcaagatt tcagaaagat cttttggctg ggatcacagt
      121 agctgctgta gcattgcctt tagccttggc ttttggggta tcaagcggag ctgatgcggc
      181 cgcaggtttg attacggcta tcattgccgg tatcgttatt ggcggtttat caggcggttt
      241 ttatcaaata tcaggtccga caggtgcgat ggcagctatc ttgatgtcta ttgcggcaac
      301 aaagggaatg caaggtgtat tgcttgctac ttttttagcg ggtctatttt tacttgcagc
      361 aggacttttc cgattaggaa cactgacttc ttttatccca gcacccgtaa ttacggggtt
      421 tacttcaggt attgctatca ttattgcatt aggtcagatc gataatttat ttggtgttca
      481 ctcggaaggt acaaacgtta ttgaaaaact aagtagttat caaacgatag gatttccgat
      541 aaacacagcc tctttgatca tgggagggct agtcatttta gggatgttta tctatccaaa
      601 aaaatgggcg aaacgaatgc ctagttcgct gctggctatc attttggcaa cagcagttat
      661 gctattgttc catttaccag tagcaactgt gggtaagatt ccacaaacat tgatcagtag
      721 taatcgtttg aatatgggag attttagctt ttctgccttg caacaagtgg cagttccagc
      781 tattagcatt gctttattag gaatgattga aagcttgcta tgtggtgctt cggctggccg
      841 aatggcaaat aagcccttag atagcaatca agaattaatg gcgcaaggaa ttggaaatct
      901 gttgcttcct ttctttggcg ggattcctgc tactgcggcc atcgcccgta caagcgtggc
      961 catcaaatct ggtgcacaaa ctagattagc tggtatgata catgcagttg tcttactgct
     1021 ctccatgctt gtttttgcgc cagtgatgtc acagattcca atgccagcac ttgcaggtgt
     1081 tttgattgtg acggcttggc gtatgaatga atgggaaacg atcaaggagc tatttactaa
     1141 taaatattgg tcagcagttc tattgtttat ccttacgatg atttgtacag ttatctttga
     1201 tttaagtatt gctatcgtga tcggggtgat cggcggctgt gtattttttg ttgtcaaaag
     1261 cgctgctatc acgatttcat tagaaacgat cgattggcaa agaatggggc tgccagaaac
     1321 ggaaaagctg aataactgga cagtagtgta tattagtggt ccactgtttt ttatgtcttc
     1381 agaaaaactc aaaaataaat tggaagaact aaatcatcac gatggtgtca ttttctccat
     1441 gcgaggcgta ccaagtattg atttgacggc acgtgcagtt tttgatgaat ttcaagaaaa
     1501 agcagagcaa agaggacaaa cgattatcta tacttccttg cagccagaag tagaaaaaca
     1561 actttctcac ttgtgggaac atacattcac gcatcaacat caaacagttg ctcatgcgtt
     1621 gacagcttta catgcgaaat atactgcaat tagccattaa aacaatgaaa aagaacagac
     1681 aagactgtaa actgaagatt ttatggtctt gtctgttctt tttaatattg ctgtaaaagt
     1741 gaagctagta cttgccagtc ttgatggata acaggtacaa gggagcgatt gtagaaaata
     1801 gctgctggat gaaaaagtgg aagaattcga tagttttttt ctgaccattc gtaggcatcc
     1861 ttttgttctg ttaattgtaa gacaggccct gtatagagtt gaccatgatt tttggtaacc
     1921 gtatattctt tgccaagtaa acgttgaaga ccaatgtttc caagtgtcgc aattaatttt
     1981 ggctggacct tcatcagttc gtaatccaat attggtgcat gcgcaaatac ttcggaacga
     2041 gtaggtgtcc gatttggata aacagtttct gactgttgtg tccgtttatt gatccgatga
     2101 gtcacgcgat acgggcggct ccttacagca ctggtgatat acacctcttc tcgtgttaag
     2161 ccagttgaag aaagtgcctg catcaattct tttccagctt gaccgctaaa aggaacatgc
     2221 gtatcaatct ctgttttccc tggggcttct cccacgagca tcagtttagg atgaagaggt
     2281 ccttgcccag cgataaatcc ttctacagga cggccgttca ttcttaaaga agcttcttgt
     2341 atcagttctt ttgggtattc catttatgct cctccttttt cttttattat aagaataaaa
     2401 gaaacacaat gcgactattt tatttggtag aactgtgagg atagaaaagc agaagtattg
     2461 cttgaatttt tgttttactt tttgatttta aatttagttg actaacggtt gctgttctgg
     2521 taagctagta ctgttgtaat tgtggactcc acagctacaa ccgctcagaa caagtaataa
     2581 gcacagcata tgctgtcact tcggatggcg agtctaagta tatcagaagg aggtgcttta
     2641 tagcatgtac gcaattatta aaactggtgg taaacaagta aaagttgaag tgggacaagc
     2701 aatctatgtt gaaaaacttg acgttgaagc aggagaaaaa gttgtttttg acgaagttat
     2761 cttagtaggc ggagaatcta caaaagtagg cgctccaact gttgcaggtg caactgttga
     2821 aggtactgtt gaaaaacacg gcaaacaaaa gaaagtcgtt actttcaaat acaaacctaa
     2881 aaaacattca caccgtaaac aaggtcatcg tcaaccatat acaaaagtgg ttatcgacgc
     2941 gatcaacgct taatagcgtt tgagaaagaa ggcctatata tgatcaaagg aacatttaag
     3001 cgaaatgacg caggtcagat cgtttcattc actttgaccg ggcatgctga tgctggtcct
     3061 tatggaagtg atatcgtttg tgcgggtgtt tctgcgttag cgatcagcac tgtcaacggg
     3121 attgcctcat tagccggttt tgagccgatt gttgaaatga atgaagaaga aggcggttat
     3181 ctttataccg aagtcacttc aggaatgaca caagaacaaa acaatattgc tgagattcta
     3241 ttagaaaatc ttttattagg attacaatcg atagaagctg aaaactcaga atatatccaa
     3301 attaaaacaa ttaatgataa ataggaggtg cagaccatgt tgttatctat gaatctacaa
     3361 ttatttgccc acaaaaaagg tggcggttct acatctaacg gccgtgactc agaatcaaaa
     3421 cgtctaggtg ctaaacgtgc tgatggacaa acagttacag gtggatcaat tctttaccgt
     3481 caacgcggta caaaaatcta tccaggtgta aacgtgggta tcggtggaga cgacacttta
     3541 tttgcaaaag ttgatggcgt agtacgtttc gaacgtaaag gccgtaacaa aaaacaagtg
     3601 tctgtttatc cagtagctca agaagcttaa ttgaatctta tgattaaaga ggttgtgaca
     3661 agacttttgt cacagccttt tttgtttata aaaataaaag ttgtagtgga ggctacgaag
     3721 tttactttgt ctttttacac ttaatatttt tttgccaatt tggaatcagg atgttttgat
     3781 aactatcgtt ttcaattgat ggaagctata ttgtttgcta tcagtgaaat aattatatgg
     3841 taaacagtgt tctttttctt gtttccatta taaaaatcgt atcgggattt ttcgttcttt
     3901 taaattgaac atttctatct atccgcgtgt tatcaacaaa acaaaatcag gtaaatagtt
     3961 gacgaaacag aaataaatac atagtattat gttttgaagc ctaagtacaa tagtctatag
     4021 aaaaggagcg ataccattgg caaaacgcat tgaccgaatt tatatgtatg tcaaagaaaa
     4081 aaccgctcat ttaacaaagg cagaatatga tcaaggagta acaacacagg aaattgctga
     4141 agtgttaggg attcagcgaa caaatagcag taaagatctg aatcagctag tgcgcgaagg
     4201 aaaactgata aagacagacg gacgaccagt acgttacata tatcaaacac tcgttacgca
     4261 cgtaaagcca gttacaaaac atgtagtcag ttacaaagaa acgccggtct ctgaagaaaa
     4321 aaatacactc caaatagata ctaaggatat ctttgcaaaa atcattggag ctaacggcag
     4381 catgaaaaat tcagtggaac aggcaaaagc tgcgattttg tatccgccaa gaggattaaa
     4441 ttgtttgatt acaggaccta ccggatcagg aaagacttat tttgcacacg cgatgtatca
     4501 ttttgcaaaa gcgcatcatg taatagatga ggaaacggaa ctgattgttt tcaattgtgc
     4561 tgattatgcc aataaccctg agcttttgat gagtcattta tttgggtacg caaaaggtgc
     4621 ttttacaggc gcagatgaag aaaaaatggg gattatcgat caagcggatg gcggtatgct
     4681 gtttttggat gagattcacc ggttgccgcc tgaaggtcaa gaaatgatct tttactttat
     4741 ggatcatgga acttacagtc gtcttgggga aacaacgaaa tcccatgaag caaatgtacg
     4801 gatcgtaggt gcgaccacag aagacccagg ttcttctcta ttagaaactt ttgttcggcg
     4861 tatcccaatc aatatcaaac ttccggcttt tgaaaaacgt cctgcaaatg agaaagtcga
     4921 tttagtaaaa atcatgattg cccacgaagc aaaccgcacg cagcgaaaga tttctttgac
     4981 agaagatgta gcaaaagcat tgatcggcag tgtgacctat ggaaatatag gacaattgaa
     5041 gtcaaatatc cagttagtat gtgcacgagg atttctaaat catatgcaat cacccgaaat
     5101 ctcgatcaca atcgatgatt tgacagaagg gattcgtagc ggattgattc aattagcaag
     5161 caaccgcagt gcaatggcag agctttctaa aatattggag ccaaagataa ctgtagcacc
     5221 aaatgatacg atcatgaaaa tccaatcgga ctcctatgag ttaccttata atctatatga
     5281 tatcatcgga gataaagcag ctttactgaa gtcggatggt ttggatcaag aagcaatcaa
     5341 tcatttcatt tctacagata tcaacatcca tctgaagtct ttctataaag accatggttt
     5401 ttcttttaat gcagacagca aattagcaga atttgtagat ccgaaaatca tagaagtgac
     5461 caatcagatt tatgtaatgg tcaaaagatc tttaccatat gagtttcagc aaaactttat
     5521 ttatgctatg agcttgcata tcagttcttt tttgaaaaaa atcaacttag gagaagaacg
     5581 gcatacgaat gataacatcc gtgagatggc ttttgatttt ccagatgaat atgctgttgc
     5641 gaaagaagtg cgtagttata tcgaaacgta ttttcaagtg gagattccag aaagtgaaga
     5701 ctattacttg accgttctac tcgtctcgtt acgggcaaat caagcaagcg gcagaatcgg
     5761 tgtagtcgtt gcagctcacg gaaacagcac agcaagcagt atggtccaag ttgtccaaca
     5821 gttgttagat gcagagaacg tacgagcagt agacatgcct ttggatatgg atccgaaaac
     5881 agcattggac cgaattgaac gcaatgtaca agaagtagat gaaggaagcg gggtcatact
     5941 cttagtagat atggggtccc ttgcgtcgtt taattcgcaa attcaaagag acactggaat
     6001 tccagtacgt actgttgata tggtaacaac ttctctagtt ttggaaactg tacgaaaggt
     6061 ctcagtatta gggacagatc tagatatgtt gcatgaatca ttgaaaaatt ttcgaggtta
     6121 cgcagaaata tcttctgtag aatctgttca ggaagcaaaa cctattcata agaaaaaagc
     6181 tgttttggct atctgcgcat ctggcgaagg tacagcacaa agaatcaaag aattgatcga
     6241 acgagcggta aacaagcgtc aagaaacaga attagaagtt ctagctttgt cgattgtgga
     6301 agtgaaagaa gaactgccag ttatccaaaa agaatatcag atcatagcta ctactgggat
     6361 tactgatttg aatatccaag ccccatttat tcctttagaa cgttttattg atcaaaatat
     6421 cgaagtcatt ttggatcagt tgctgatgga atcagaattg gaggaaactg aattcatctc
     6481 cctagatgaa gagtcagcta aaaatacgtg tgttgagttt atctctgata acttcatctt
     6541 tattaatggt agtaagttaa tcgatccaat gtggcaattt tctacacaaa tctctcagac
     6601 gacgggaatc ggcgatgaag aatatggatt caaaatcaac ctagtgatgc acacagcagg
     6661 aatgatcgaa cgtattattc ggaatgaacc attgactgtg gaagaaaacg aattgacaaa
     6721 tacaacgaat gatcctcttt atagccaatt ggcagcctca gttgttctgt tggaagatca
     6781 gataaaagta aaagtgccga tagaagaaat gtattatctg ctacgtctag tccataatca
     6841 gttagataaa aaagaataca ctgtgccatg aatacacaaa aacgatacac ttttatagtg
     6901 tatcgttttt gtgtattggt taagaatcct ttaaattcaa ttagtgtatc gtaaaaaagt
     6961 tggcacgctt tttgcttata gtagtgtgtg ctaggaggtg gttgttcaca cagaaatgat
     7021 aggggtttct gtgaagtagt gaggaagaag gagttttgca gatggatatt cgattggcac
     7081 gtatagatga tcgtttaatc cacgggcaag tcgcaacagt ttggtcgaag caaacaggtg
     7141 ttgaacgtat cattgtgatc agcgatgtag tcgctcaaga taaattgaga aagtttctac
     7201 taaaagaagc tgcaccgccg gggatcacag caaatgtaat aaccgtgcag aaaatgattc
     7261 aaatctatca tagtggatta ttatcacgag caaaagtgat gcttttattc accaatcccc
     7321 aagatgttga agcggttgtc cgctgcggtg tacagttgaa gtcgttgaat atcggcggta
     7381 tgagttttac tgaaggcaaa tcaatgatta ccaattttgt ttcagtaaat caacaagata
     7441 ttgattcctt tcgttacttg aacagtcaag aagtggagtt agagattcga aaagtacctg
     7501 ctgacaggaa aatccttttg atggaactac tagcgaaaat caaaaaggtg tagcaagaat
     7561 ggttgttgga aaaggtcaaa taattctgtt gtaatggtta gcaattatta atatttttga
     7621 atttgttaca acaaaagtaa ataggaggta taaatatggt agggattatc ctagcaagtc
     7681 atggacaatt tgctgaagga atcttacaat ccggttcaat gatctttggc gaacaagaaa
     7741 acgtcaaagc cgtgattttg aaaccaagtg aaggtccaga tgacttgaga gccaaattgg
     7801 aagaagcggt tgcttctttt gataatcaag acgaagtgtt attcttagtt gatttatggg
     7861 gcggaacacc gttcaatcaa tcaaacacat tattcgaaga gcacaaagat aaatgggcaa
     7921 tcgtcagcgg tttgaattta ccaatgttga ttgaagccta cgcatcacgc ttttcaatgg
     7981 aatctgctca tgaaattgca gcacacatca ttgaaacagc aaaagacggt gtaaaagtaa
     8041 aaccggaaga attagaaccg gcagaagcac cgaaagcagc agttgaagac gcacaaccta
     8101 aaggagcact tccagaagga actgtggtcg gagacggaaa aatcaaatat gtattagcac
     8161 gtgttgactc acgtctgtta catggacaag ttgcgacagc ttggacaaaa gcagtgcaac
     8221 caaaccgtat tatcgttgtt tcagatgcag tatcaaagga tgatctgcgt aagagactaa
     8281 tcgaacaagc tgctcctcca ggtgtaaaag caaatgttat tcctattagc aaaatgatcg
     8341 aagtagcaaa agaccctcgt tttggaaata ccaaagcatt gcttttattt gaaaatcctg
     8401 aagacgtatt gacagctgtt gaaggtggcg tagacatcaa agaattaaat gttgggtcaa
     8461 tggcgcattc ggtcggtaaa gtcgttgtaa gtaaagtatt gtctatggga caagaagacg
     8521 ttgaagcgtt tgagaagctt gaacaaaaag gtgtgaaatt cgatgtacgt aaagtaccta
     8581 atgattcacg cgataacatg gatgatattt tgaaaaaagc aaaagctgaa ttagcaaaag
     8641 cgtaataaat aatattaaaa tttataggag gcttatcatg tctattattt caataatttt
     8701 agtcgtatta gttgcctttc tagctggtat ggaaggtatt cttgatgaat ttcaatttca
     8761 tcaaccacta gtggcatgta cattgatcgg tttagttacc ggtaacctcg aagctggtat
     8821 cgtacttggc ggtacgcttc agatgatcgc tttaggttgg gcaaatatcg gtgccgctgt
     8881 agcaccagat gctgcattag catcagtagc atcagcaatt attttagttt taggtggaca
     8941 aggtgttaaa ggtgttcctt ctgcgatcgc gattgccgtt cctttggctg ttgcagggtt
     9001 gttcttaaca atgatcgtac gtacaatcgc tgttccaatc gttcatttga tggatgcggc
     9061 tgctgaagaa ggaaacatcc gtaaggttga aatgtggcac atcattgctg tatgtttaca
     9121 aggggttcgt atcgcggtcc cagcagcagc gttgctattt atcccagcac aaactgtgca
     9181 atcattcctt gaatcaatgc cagcatggtt aacagatggt atggctatcg gtgggggaat
     9241 ggtcgtagct gtaggttacg ctttggtaat caacatgatg gctacaaaag aagtatggcc
     9301 attcttcgtt attggtttcg ttgtagctgc aatttctcaa ttaacattga ttgctctagg
     9361 tgctttaggt gttgcattag ctcttattta cttgaacctt tctaaaatgg gtggttcatc
     9421 aaataatgga ggcggaagca actccggtga ccctttaggc gacattttga atgactatta
     9481 atcttgaagg aggaaaataa aatggcagaa gaaaaaatca aattatcaaa aaaagatcgt
     9541 ttagctgttg cgtggcgctc taccttcatt caaggttcat ggaactacga acgtatgcaa
     9601 aacggtggct gggcattttc tatgattcca gcaatcaaaa aattatacaa aacaaaagaa
     9661 gaacgttctg cagcattaaa acgtcacttg gaattcttta atacacaccc atacattgct
     9721 tctccaattt taggggttac attggctctg gaagaagaac gtgcgaatgg cgcgcctgtt
     9781 gatgatgtag cgattcaagg ggttaaagta ggtatgatgg gtcctttagc tggtgttggt
     9841 gacccagtgt tctggtttac agtacgtcca atgctaggtg cactaggtgc ttcattagct
     9901 atgggaggaa atatccttgg accaatcatc ttcttcttag gttggaacat tattcgttgg
     9961 gcattcatgt ggtatacaca agaatttggc tacaaagctg gttctaaaat tactgatgac
    10021 ctttctggcg gattgttaca agacgtaaca aaaggtgctt caattttagg gatgttcgta
    10081 ttagctgccc ttgtagaaag atgggtatca atcaaattct taccagttgt atcaactgta
    10141 aaattagata aaggtgctta catcgaatgg gataaattgc cttcaggtgg tgaaggaatc
    10201 aagagtgcat ttgaacaagt taataatggc ttagcccttt ctcctgaaaa ggtaacaaca
    10261 ttacaagaca acttagacca attgattcct ggtttagctg cattaggatt aacattgtta
    10321 tgtatgtggt tgcttaagaa aaaggtttct ccaatcgtta tcatccttgg tttattcgta
    10381 gtcggtgttg ttggacacgt catcggactt ttataatcca taaaaatata gctcgggcgt
    10441 tgtcccgagc tttttttata tagtatactg ttcataaaca aatatctgga gggagaaaat
    10501 ggttcaatca atcaatacaa aagtagactt ggtaattgat gctacttcgt ttacgggtct
    10561 gacagattac gggcaaatca tgataggtga taatggtttt gagttttacc atgcgcgaga
    10621 tagacgcaaa tatatccaaa ttccatggga agaagtggat tatgtactag catcagtcat
    10681 gctaaaggga aaatggattc ctcgatatgc cattcaaacg aaaaaaaatg gtacatacac
    10741 attttcttcc aaagaaccta aaaaagtgct tcgtgcgatc agagaatatg tagatgaaga
    10801 tagaatggta caatcattga gtttctttga tgtagtgaaa cgtggtattc gtagtatgtt
    10861 taaacgcaag taaaaaataa agaaaacttc taagaggagg ttttctttat ttttttactt
    10921 tgcagaagaa aatatgaaat agcaaaacca attatttgtc ccctttaaat cccatatgct
    10981 aaaattgatt agcaaagaag gaggttatat tatgagttgg ttatggacat tgattgttgg
    11041 agccgtgatc ggagcaattg caggtgcgat caccagtaaa ggaaattcga tgggctgtgt
    11101 aacgaatatc attgctggac tagtcggttc aagtattgga caagcgattt tcggcgaatg
    11161 gggaccaaaa gctgcaggta tggcacttat tccatctatt ttaggcgcag tgattttggt
    11221 agcagttgtc tcatttttct ttggaaaacg atcataagtt tttacaaaat agtaaactaa
    11281 aaggagttgc agcgagactg tgactccttt ttggctttat ttatttaaaa taaaaagaag
    11341 ttttccggaa atttttattt aactgtcgtg tacgaagaaa agtttgctat aatggaaaaa
    11401 agcgtgcaaa gaaagaggga gtttcattat gatgctacgt gtagaaaaat tgcgcaaaaa
    11461 aatgcaagaa gaaaatttgg attcattttt ggttaccagt ccgtacaatc ttcgttactt
    11521 aacgaacttt acaggtacga caggattagc tgttattact ttggaaaaag cattttttat
    11581 tactgatttt cgttataccg agcaggcggc agcgcaggct caagggtttg aaattattaa
    11641 aaatgtcgga cctatttttg atgaggtagc aaacttagta cgcaaagaag aactgcactc
    11701 attagctttt gaggaaacaa cagtctcatt tttagaatat tcggttttag aagaaatcat
    11761 cgatgcggaa ttagtacctg tttcacagat gatcgaagaa ctcagagaag tgaaagatga
    11821 agaagaaatc gctattatcg aaaaagcatg tcatattgcg gatatggcat atgatcacat
    11881 cctgaaaatg atccaaccgg gcatgactga gattgaagtc gcaaatcaac tagactttta
    11941 tatgcgttct cttggcgctt caggtgtttc ttttgatacg atcgttgcta gcggattgcg
    12001 ttcggctatg ccgcatggtg tagcaagcaa aaaaatcatc gaacaaggtg acttgatcac
    12061 attggacttt ggttgctatt acgaagggta cgtttccgat atgacacgta cattcgctat
    12121 tggtgatcct ggggaaaaat taaaagaaat ttatcaaata gtcttagatg ctcaattagc
    12181 agttattgat gctgcaaaac caggaatgac tggtgtccaa ttagatgcag ttgctagaga
    12241 ttatatttca aaacatggtt acggggaagc atttggacat tctacaggac atgggatcgg
    12301 tttagagatc cacgaaggac caaacgtttc ttctcgtgca gaaaagcaat ttgtaccagg
    12361 aaatgtgatc actgatgaac cagggatcta cttaccagga atcggtggag tacgtattga
    12421 ggatgacttg ttgatcacag aagatggaaa tcgtgtgttg actcgttcac caaaagaatt
    12481 gatcatctta tagtttcatc aatagaagaa gtaagcgtaa aaatcagtgt tcacagactt
    12541 tgttatagaa aatcactcga tttaatgata cactatagaa gaacttttaa gctgtaagag
    12601 ataataaagt tgggtgtttt ctccaattca tagcagtttg cagctgatcg gcggcagttt
    12661 tcttaccacg tgatcactat gccgcatcat agagaattcg gatgtatctc gtgcttaggc
    12721 aaagggagga ataaataaat ggcagatgaa aaaaatttga ttttaaacgc aaaccaagat
    12781 ttaggagaga tcgtgatcgc tcctgaagtc atcgaagtaa ttatcgggat tgcagcttct
    12841 aaagtagaag gcgtatacgg aatgcgcgga acttttgcca gcaatgtcac tgaattatta
    12901 ggacgtgcag ctcacggaaa aggtgtttac ttagcaaacg aggaagatgg cttgaaagta
    12961 gatctttact gttatctaga atatggtgtt tcagttccta aagtagcaat ggaaatgcag
    13021 gaacgggtca gccagcaagt ccttttcatg acggatatcg atttatctga agtcaatatc
    13081 catgtagtag ctgttgttcc tgaaaaatta gaacaaccaa caatcgaaga actttttgaa
    13141 gacgaagagg aagagaatga ataaagaatt atcaagacac gagattcggg aaatggcatt
    13201 gcaggcttta tttccattag acttcaatgc agacctaaca aaagaagatg cgattttcaa
    13261 cgcaatcgaa ttagaccacc gcgacatgat caacgaagat gaatcagagt ttgttcctgt
    13321 ttatcttgat acgctagttg gcggtgtctg tgcaaaaaaa gatgaattgg ataaagtgat
    13381 tgaaaaacat ttaaaaaaca attggcacat caatcggatc tcaaaaatgg atttggtgat
    13441 cttgcgtatc gcgatttttg aaatgactta tgtgacagat gtaccagcgg ctgttgcttt
    13501 aaatgaagca atcgagttgg caaaaacatt tagtgatgac cgttcacgta aattcgtcaa
    13561 cggtgtttta tccaatgtat tgaaagaatt agaagctggg gcgtaagtcc tagctttttt
    13621 ctatgaatca tcataaacct gttataaatc gggaaaattc tatggtagaa tgaaaacaca
    13681 aaaaatatct gggagtggtt agatggcaga attgattaac ggaaaagaat tagctgaaaa
    13741 aatgcaggca gagattgctg aaaaaatcca aaaattgaaa gataatggta tccatcccgg
    13801 attagtcgtt ttattggtag gggaaaatcc agccagccaa atctatgtaa gaaataaaga
    13861 acgtgtagca aaagaaatcg gaatccattc attggtagag cgttatcctg agacgatttc
    13921 tgaagacgaa ctacttgcag aaattgaaaa atacaatcaa gatcctagtt ttcatggtat
    13981 attggtccaa ttaccattgc caaagcatat agatgaagaa aagatcctgt tagctatccg
    14041 ccctgaaaaa gacgtagacg gttttcatcc gatgaattta ggcagactcc ttgcgggtaa
    14101 accggacaag attccttgta ctccatacgg catcatgaaa atgtttgaag cttattctat
    14161 cgatccggcg ggtaaacgag cagtagttat cggacgaagc aatatcgtcg gtaaaccgat
    14221 ggctcagcta ctcttgatgg cagatgcaac tgttactgtt gctcattcta aaaccgaaaa
    14281 tctggctgaa ctggcacaag aagcagatat tttagtcgta gcaataggac gtggacattt
    14341 tgtcacaaaa gaattcgtaa aaccaggtgc tgtagtgata gatgtaggga tgaatcgtga
    14401 tgctaacggt aagctgatcg gtgatgtgaa atttgatgaa gtggagccta ttgcaggctg
    14461 gatcactcct gtgccaaagg gtgtagggcc aatgactatc acgatgctga tgtatcaaac
    14521 cgtaaaatct gccgaagctt ttggagcagg tgaatagatg gcacaagaat atttaactgt
    14581 cactacatta acgaagtatt tgaaaagaaa atttgatgcc gatccttatt tagaacgcgt
    14641 atatttaaca ggcgaaatat cgaatttccg tttacgtgcc aatgcccatc aatatttcag
    14701 cttaaaagat gaccatgcaa aaatatctgc tattatgttc aaaggagcat tccaaaaatt
    14761 acgattccag ccaaaagaag gaatgaaagt ccttgtcgtt gggcgtattt cattatatga
    14821 agcgggaggt tcttatcaaa tctatgtaga gcacatggag cctgatggaa taggtgcttt
    14881 atatcaagaa ttggaagaac gaaaagaaaa gctgatgaag gaaggccttt ttcaagggcc
    14941 taaaaaaatg ctgccacgtt tcccaaagcg aatcgcggtc cttaccagtc caagcggagc
    15001 ggttatccgc gatatcatca cgacggtgaa aagaagatat ccaattgtcc aattggtttt
    15061 gttcccaaca gttgtacaag gaaaccaagc agcggacaat gttgtcagga atatccagag
    15121 agtagaagag atgggcaatt tcgatacaat gatcatcggt cgtgggggtg gttcgattga
    15181 ggatttatgg ccgtttaatg aagagcgggt tgctcgggcg atttatcaag ctaatacgcc
    15241 ggtgatttca tctgtaggac atgaaacaga taccacgatc gctgatttag tagcagatgt
    15301 tcgggctgcc actccaacag cagctgcaga attagctgtt cctgttttgt cagaagagtt
    15361 gatgcgcatt caagaacggc aagctagatt gcagcaggcg tttgtacgcc agatccagcg
    15421 caaacaagaa cggttcgagc gtgctcgacg ttcctatgta tttcggcagc ctgaaagatt
    15481 gtatgaaggt cagaccatca aattagacca gttgaaacag cgattatatc aagctacaca
    15541 gcaaatttat catgcgaaag aaaaacaaac gatcgcatta agccaccaat tggaacaagt
    15601 ggctccgctt tatcgagtta aagcagctcg ccaagaaact gcttatctag aaaaacgctt
    15661 agaagagaaa atccaacaat atatgaaaca acagcagcag cgttttgctc aagctgttca
    15721 atctcttgat ttgttgagtc cattgaaaat catgggaaga ggctacagct atacgacgtt
    15781 aaatgatcaa gtagtaaaaa gtgtcaccga gattcatcaa ggggatttgc tgacgatcca
    15841 ttatcaagat ggaatcgtta aaggacaagt acaagagata gacaaaggag aataaaaata
    15901 tggaaaatcc aacttttgaa gagtcactgc aagaattaga aaaaattgtc atgcagttag
    15961 aacaaggaga cgttccatta gaatcagctc tagattcgtt taaacgagga atggaattaa
    16021 gcaaacattg ccaagataca ttagctaaag cagaagagac cttgacgaaa atgatgacag
    16081 aatcaaatga agaagttgat tttgacggaa acgaggaaag ctgagatgag attaactgac
    16141 ttttcagcta ttcatctacc tgctgtagaa aaagaaatct tgtctttttt agatgagcat
    16201 acaacggata gaggactcta tgatgcgatg agttattcag tgaaagcagg cgggaaaaga
    16261 atacgtccct tattactttt aacggccgtt gcttcctttg atgagcctat tgatgttccc
    16321 gtctatcaag tagcagctgc tttagaaatg gtgcatacgt attccttgat ccacgatgat
    16381 ttgcctgcga tggacaatga tgatcttcgt agaggaaaac ctaccaatca taaagtattc
    16441 ggcgaagcat tagctatatt ggccggagat ggtttgctta caggagcatt ccagctaatc
    16501 agtatggcac acttaggtaa ttctcctaag cttctgctgc ttcagcaatt agctgtttgt
    16561 gcgggatcac aaggtatggt tgctgggcaa gctgcagata tcgagggcga atcgaaaaag
    16621 ctttctttag aagaattagc atttattcat gaaagaaaga cagggcattt gattcgttat
    16681 gccttgctgg ctggtggcat attagcgaag caacctgaag aaatcttatt gctacttcaa
    16741 cgattagccg aacatttagg tttagctttt cagataagag atgatctact cgatgtgatt
    16801 ggtacaacga aaacattagg gaaaacagct ggtaaagatg aacgtatgga aaaaaatact
    16861 tacccgcgtt tattaggcct agaaaagaca cgtgaggcgt tggagattga gctgttatcc
    16921 gccaacaaga tcatagacaa attagaagag gacgtttttt cttttgatgg cgggctgatg
    16981 cgccagatga tcaagcaatt tgatgtggag aaatgaggga aagaatggaa aaagaacgcg
    17041 tagatgtatt agcggtcaaa caaggattat ttgaaacaag agaacaagcc aaacgttcag
    17101 tgatggctgg tttgatttac aacgaaaaaa acgagcggtt tgataaaccg ggagaaaaaa
    17161 ttccagtgag tagtgaatta agagtcaaag gtaaaaaact tccttatgtt tctcgagggg
    17221 gattaaaatt agaaaaagca ctcaaagcat ttgatctatc tgttgaagga aaaacattgt
    17281 tagatattgg tgcatccact ggcggattta ctgatgcagc tttgcaaaat ggcgcaaaaa
    17341 tgagttatgc tttagacgtt ggctacaatc agctagcttg gaaaataaga caagatccta
    17401 gagtcgtcgt aatggaaaga gtgaattttc gttatgcaaa acccgaagac tttaccttag
    17461 gaattccaga agttgctgtt atcgatgttt cttttatttc actaaaactg atgctgcctc
    17521 cattacatgc tattttaaaa gaagagggag aagtgattgc tttaatcaaa ccccaattcg
    17581 aagcaggcag agaagctgta gggaaaaacg gaattgttcg tgaccctcag acccatcaaa
    17641 aagttttaga agacattttg tcatttgcgg cacaaggtgg atatgatgtg ctggaattaa
    17701 gttattctcc gatcacgggg ggagaaggaa atatcgagtt cttggctcac ttgaggaaag
    17761 tacccgaaag cggaacaatc aattcagcta ttaatatggc agaagtggtt tcaaatgcac
    17821 acgaacagtt tgaccataaa taaattgtaa ctgtgaaaat caagtgagaa gtatgcggat
    17881 tcttctttat tataccaagg aaataagcta tcattgatta agaagaatat gctaaggagc
    17941 gaagacgtaa tgagaaaaaa agatcggcat cgtttgatta ctcgattgtt gagtgagcaa
    18001 gatatccgaa agcaggaaga atttgttgaa atcctgaaaa gcaaaggaat atctgttact
    18061 caagctacga tttccagaga tataaaggaa ctgaaactga tcaaggtacc ggcttcagat
    18121 ggcggctacc gttatagcat accagctgaa acaaacgaag atgtaaatac taaattggaa
    18181 aaactattaa aagacgcttt cgtggcagtt gaccaaatgg aaaaatttgt tattttgaaa
    18241 acattgccgg gaaatgcttc ggcagcagca aatttgattg ataaacgtta caagaaagaa
    18301 ttattttcga ttatcaatga tgacgataat gttttgatga ttacacgaac tgaagaccag
    18361 gcagttttat tgaagaaaga cttcctgcat tacctttaag ttgaggagcg aatgtgatgc
    18421 tacaagaaat cagtattact aactttgcga ttattccgga attgcggctt tcttttcacg
    18481 aaggaatgac cgcgctgacc ggagagactg gtgcgggtaa atccattatt atcgatgctt
    18541 taggtctgtt agcaggagga agaggttcaa gcgattatat acgtcaagga gcagaaaaat
    18601 gtgtgcttga agggttgttt gaactaccaa aacaagaagg attctctgaa ctgatggttg
    18661 aattagggat cgaaacagat gaggacaatc tgattgttcg acgggatatg tccttaactg
    18721 gaaaaaatgt ttgtcgagtg aatggtcata ttatcacatt agctaatctg agaagaatcg
    18781 gcagttattt agtagatatc caaggacaaa acgaacatca ggaactgctc cagcctgaat
    18841 cacatcttgc tttattggat cgttttggag atgtagcttt ccaacagaag aaaaagagtt
    18901 atcagcaaga atatatctct tatcgtgagc tggaaaaaag ggtacgaaaa gtgcagcaaa
    18961 atgaaaaatc ctatgttcag agaatagata tgctccattt ccagcaagaa gaaatagctg
    19021 ctgctgattt gcaagtcgga gaagaagaga aattaaaaga agaaagagaa aaacttagca
    19081 attatcaaaa aattgctgat ggattagctg caggttacgg agcactgacg aataatgaac
    19141 aaaacagtgt agatggtgtt ggcttggccg tttcagaact gcaagggatc gcccatctag
    19201 atgtggagta tgaagcaatt tatgaaaata tccaaagtgc ttactatttg ctgcaagatg
    19261 caatcggcga tatgagtcgt cagattgatt tattggagtt agatgagagt cgtttagaag
    19321 aagttacgca acgtttagaa ttaatccgac agttgaaacg taaatacggc gaatccattg
    19381 agtcaatatt ggcttactat gacgaaatca ccgaagagct agcttcttct gatttttcag
    19441 aaggtcaatt ggacaaaatg aaagaggaac tagagcaaaa agagcttcta cttcagcaac
    19501 aagccgcaga tcttcatgaa gcgcgtaaaa aaatcgcaaa agaattggaa aaatccattc
    19561 tgcatgaatt gaaaagtctg tatatggaaa acactgagtt tgaggtacgt ttcttaaaag
    19621 aagaaaatag acaattgaat catgatggat ttgaccaaat cgaattttac attacgacca
    19681 atccaggaga gccactgaag cctttggtga aagtcgcttc tggtggcgaa ctgtctcgga
    19741 tgttgcttgc tttgaaaact attttttctt ctgagcaagg tgtgacaagt attatttttg
    19801 atgaagtgga tacaggtgtg agcggaagag ttgcacaggc catcgcagat aaaatttctc
    19861 aaatatcgaa atactctcaa gttttgtgca tcactcatct gcctcaagtt gctgcagtcg
    19921 ccgactatca atattatatt gtgaaagcag tgattgatgg acgtacgcaa acttctgttt
    19981 cagagttgaa aacaaaagaa agagaagaag aaattgcacg tatgctggct ggaagtgaga
    20041 ttacaaaact cacactcgaa catgcaaaag aactattgaa actcgcaaaa aggacctgag
    20101 ccacctcgca gtggttcagg tccttttcag cagctgatag ctatatttta tttttacatg
    20161 acactcatta cagcagtcaa tacaacagat ccaatcgtta aaatcagcat tgtccaaatg
    20221 acaaattttg ttactttagc aaaagtactt gtttgttttt tttcacgcat tgttttcaac
    20281 ctcgtcatta attagttgat tcaagtgtac aatctttttt ccatttccac aagagggctt
    20341 ttttataaaa acttcccttt ttttaaataa taggtagtta cgatacaaat aaataatgtt
    20401 aatagaacta gccatataaa ggcatcatcc ctttcagcaa taggtaactt aatgttcatt
    20461 ccatagatac tgccgacgat tgtcggtata gttaaaataa tcgttaaaga agttaaaatt
    20521 ttcataatat tattcagatt attcgatacg attgctgaaa aagtctgatt catttgttct
    20581 actagtttta attgaatgcg agaagtagtc attgcctgct ctgtttccac caatacatca
    20641 cgtaaatgat ttttcaaagc atgattttct tggaaaagtt tattttgcgc tagttctttc
    20701 aatgcatcta aattagcaga agtcgcttct ctgaaataaa cgaggctttt ttggatatcc
    20761 atgatttgat aaagttgttt gttttcagtg gatacttgaa gtttggcttc caaagtatcg
    20821 cattgagatt gaagcgtagc taaataactg ttgtaagaga gtgtgacctg ccaaatgaat
    20881 tgaaagaaga tattgagatt aggcgaaata tcactttttg gaaaggtctg gctaaagact
    20941 ttctttaaaa acagtggttc atgattcgta attgtgataa gcttcttttt gggcgtcaaa
    21001 ataatagaga gcgggtatgt atccacttga aaataaccgc taggacttgt tttagcaaaa
    21061 ggatattgta acaataataa cgctggcttt gtcaactttg attgattgaa ttcttctgta
    21121 cgggaatttt ctgcatcgtc caatatggaa gtgatgtaat ctgacggaat ttcaaattga
    21181 tgtgtcagct gatgaatctc ttcttgatta ggacgttcta cacaatacca gatcgtttgg
    21241 tcagtttcct ttccggtatt acgcagtttc tcattatcta atgtaaaata tttgatcatg
    21301 attttcctcc tgttgcctgt ttcttttatt ataacggata gggaattgga atacatggaa
    21361 tgtgtttgta agatatagaa tataactgca taaatttaaa taaaatgaaa cagttgtgta
    21421 aagtatatac agtatacttg aagcaacgat aaaaaaggag tttacgttca tgggcacatt
    21481 cacaccactt atcttgtttt ggtatattta cccggtaatc gttttgtttg gttgccaatt
    21541 tcttgtaaaa ttattttcgt taaatcgtcg ttttaaaata aaagcccctg atttggcgat
    21601 tccttttttg tggggcggta tgcatgcatt gtctagaaat acagggacaa tttctatctt
    21661 accgtttttc tttatttctg ttttgctaat gggaatttgt attgctgttt tccaagcata
    21721 ttattatgaa gaaattattt atccgcgtta tttcaagatg acttggcgtt tggtttttct
    21781 gttgacaatc gtcttgtacg ctgtcttgat cattgtgaac atactttctt acctatgaaa
    21841 aaaatagaaa agctgtaaaa agtcttttaa aaggcttttt gcagcttgat ttttaaaaaa
    21901 atgtttatga ataaaaaaag ttgttcccgg atttcccaga aaaggtggta gaaagtgggg
    21961 gattgtggta gactaatttt agttagtgga ggaatggggg gctttcaatg ttgatgggtg
    22021 aattccaaca taatattgat gcaaaaggac gtttaatcgt gccctcaaaa cttcgtgaag
    22081 aacttggcga aaagtttgtt ctgacacgcg gattggatgg ctgtcttttc ggctatccga
    22141 tgagcgaatg ggaaaatctg gaagctaaac tgaacgaaat gccgttggct aaaaaagacg
    22201 cacgtacttt tgtgcgattc ttctattcgg ctgccactga atgcgaactt gataaacaag
    22261 gacgaatcaa tatacccagc acgttaagaa attatgcagc tttgacaaaa ggttgcgtag
    22321 tcatcggtgt atctaaccga attgaaattt gggatgagac acgatggcaa gatttttctg
    22381 cagcagcaga agagaacttt gacgaaattg ctgaaagtat gattgatttc ggactataag
    22441 aataggaaga ggctgactaa tgactgaaac atttcaacac tatacagtca tgttgaaaga
    22501 aacggtagat ggattgaaca tcaaaccgga tggcgtttat gtagactgta cactcggcgg
    22561 tgccggacat agtgaatatt tattaagcca actaagttct aaagggcatc tgtatgcgtt
    22621 tgaccaagat caaaaagcaa tcgatcacgc caagctgcgt ttagcttctt atgtcgaaca
    22681 aggtatggtc acatttatca aagcgaactt cagagaatta gaagaacgct tgagtgagca
    22741 tgtagataag gtagacggca ttttgtatga tctaggggtt tcctctcctc agctagatga
    22801 agcagaacgt ggatttagtt atcatcaaga tgcaccgttg gatatgcgca tggatcagtc
    22861 agctccttta tccgcttatc acgtaattaa tgaatattcc taccatgaat tagtaaagat
    22921 tttcttccgc tatggcgagg agaaattttc caaacaaatt gctcgtgaaa tcgaacgagt
    22981 acgaaaaaca aaaccaatcc aaacaactgg tgagcttgtc gaaatcatta aacaagtcat
    23041 cccagctccc gcaagaagaa aaggcgggca tcctgcaaaa agaatttttc aagctgtgcg
    23101 gattgcagta aatgacgaac tcggtgccga agaggcctct ttagaacagg caatccggtt
    23161 attgaaagtc aatggaagaa tcagcgtaat cacattccat tcattggaag acagaattgt
    23221 caaaagtatg tttaaagaat atagtacggt acaagatcta ccaccgggac ttcctgtcat
    23281 acctgacgaa ttccaaccag aactgaaagt cattactcgt aaaccaatcc ttccttcaga
    23341 ggaagagcta acagaaaaca atcgatcacg cagtgcaaag ttacgtattg cagaaaaaat
    23401 aagaatgaaa gaggagtaga atgatggcag aattaaagaa aatgcaagat tacgaatatg
    23461 atttacctga attggtggat cagccggaac aacaaatgcc tgattcttct tcgaatcaag
    23521 aaatcattct tccacaatca ccggcaaaac gattaaagca gatttctgct ttggaaaaaa
    23581 ttgctgtggc aagcgtggcg attgctattg ttgctttatg tgttttaaca gttatgctgc
    23641 gcacaaatat cagcggagct gaaaaagaaa tcacgtcaat acaaacagaa atcaatgata
    23701 aaaagcaaga aaaaacgagt ttagaacaag aaaaaaacga actttctcgt actgaacgga
    23761 ttaaacaaat cgcagaagaa aaaggattgt ctattaatga cgacaattta aggaaagtga
    23821 agtaaatgag tcttaaaaat aaaattcggc ggtttatgga aaagaaaaat ctaaatccga
    23881 tgaacaatcg caaaaaagta ggtatcattt tatttgctac gagtattgga atgttctttt
    23941 tatttgccgt ccgtttctcg tatatcgtta ttggaggtca tgttgctggt acctcattag
    24001 ctgaaaaaac aaaacagttg taccaaggaa gtgaagtagt aaaagcaaaa agaggaacga
    24061 tttatgatcg taacggtgtg gcgctcgcag aagatgcctc ctcttattca attaaagcga
    24121 tactctcaaa aacatatacc tctggggata aaaaactgta tgttgaagaa aagaattttg
    24181 ataaaatagc agaaatcctg cataagaatc tttctataga caaaaaagat gcactgaata
    24241 ttttagagga cggcgcaaaa aaagagctgt atcaagttga atttggtagc tatgggaaaa
    24301 atatctccca agaaaccaag caaaacatcg aagcggacat gaaaaaagaa ggtgtcgctg
    24361 gtctttattt tgtcgaccat caggctcgga tgtatccaaa tggcgtcttt tcttctcatt
    24421 ttattggata cgcagttcca gacaaagatg agaatggctt agttggtaaa ctaggtttgg
    24481 agtctgctta caatgatatc ttgagcggta aagatggtaa gattatttat caaaaagata
    24541 attttcaaaa tcctctgcca ggtacagtgg cagaagaaga aaaagctgtc gacggacaag
    24601 atatctacac gactttagac agcagactac aaagttattt agaaacactg atggatcaag
    24661 taaatgaaga atatcagcca gaagaattga cagcagtcct gatgaaagca aaaaccggag
    24721 aaatcttggc aatgggacaa cgacctactt tcaatcctga aacaatggaa ggattgactg
    24781 gaaaagatgc tatctggcgt aatttcttgg tacaagatag ttatgaacca ggatcaacca
    24841 tgaaagtttt cactactgca gctgctatcg aagaaggaga attcaacgag aatgaaacct
    24901 tccaatctgg aaaaatccaa gtagaagatg ctacgatcaa tgaccatgac tttggtgaaa
    24961 aaggtgtgct gaccatgcgt caagctcttt catggtcaag taacgtcggg atggtcatac
    25021 tggaacagcg attaggcgga cgatggtata actatttaca aaaactggga tttggacaaa
    25081 gtactcattc tggattagat gatgaagtaa acggtgcttt acctacatta aatattgtcg
    25141 accgagcaat gagtgcttat ggacaagcgg taggtgtgac gaatttccaa atgatgaaag
    25201 ctttcacttc tattgcgaat aacggtacaa tgattcaacc tcgatatatc agcaaagtgg
    25261 tggatcccca aactggagaa gagcgaacaa ctcaaacaga agttctaggg caaccttttt
    25321 caaaagaaac aacagaaaaa gtccgtgaat atatgagaga tgttgtcgaa agcgaaaact
    25381 acggtagtgc ttatggtgta tatagtgtac ctggatacaa tgtatcggca aaaaccggaa
    25441 cagcccaaat cgcttctgat acagggggat atcaaacagg agatacagcc tacctgtatt
    25501 cgattgtgga gatggttcct tctgaagatc cagactatgt cttgtatcta acaatgaaac
    25561 accctaagac atatgatcga atggcattgg ctaaaatcgc aaatccattg atgaaacggg
    25621 caatggattt taaagaaact gaagaagatt cagatacaga aacaaaaaca gaaaaagtat
    25681 ccgtagccga ttatcgtaat cttgaagcgg acgttgcagc agctgatgct caaaaaagtg
    25741 gactgcaacc tgtagtgatc ggtaatggta aaaaagtaca aaaacaatct actgccaacg
    25801 gtgatcagtt gatctctgga gaaaaactga tcctatatac tggtggtgat aagctgatgc
    25861 ctgacgtaac aggatggtca aaagcagata tcatgaagct ggggaaaatc ctcggtattg
    25921 aagttagttt tgatggagat ggttattgtg tcaaacaaga acttgctcca tatgaaaaaa
    25981 tcactgaaga caaacttagt tttactttag aagaataaga agagaaatag gagagaaaag
    26041 agcatggaat ggacacaagc attgattccc attgtcagca gttgtgcaat gacaattgcc
    26101 gcaatgccgc tatttattgg ttattttcaa atgaaaaagc aaggtcaggc gattcgtgag
    26161 gagggaccaa agtggcacaa tagcaaagcc ggaactccta caatgggcgg cttagtgttt
    26221 ctgatcggct ctattttgac aggaatttgg gttggagctt ggcaaaaaca gctaactcct
    26281 actcttttca tcttgctttt tgtattagcg ttatacggag taatcggctt tcttgatgat
    26341 tttataaaaa tattcaagaa aagaaacatg ggactgaact cgaagcagaa attgattgga
    26401 cagattctag ggggaatcat cttttatctt gtttaccgtt ctgaaggtta tccaggtgta
    26461 ttgaatttct tcggactaga tctaccttta ggaatcattt atggtatttt cgctctcttc
    26521 tggcttgttg gattttcaaa tgcagtaaat ctgacagatg gcatcgatgg attagttgct
    26581 ggcttaggaa gtatctcttt tgcagcttac ggtctaattg cttggcatca gcatcagtat
    26641 gatgtattaa tcatttgctt aagtgtttta ggaggtttac tgggtttctt cgtctacaac
    26701 agaaaacctg ccaagatttt tatgggagat gtcggttcat tagcgttggg cggactttta
    26761 gcggcaattt ctataatgct aaaccaagaa tggacattgc ttttgattgg tttgatctat
    26821 gttatggaaa cagcaagtgt gatgcttcaa gttacttcct ttaagcttac aggaaaacga
    26881 atttttaaaa tgtctccaat tcatcatcac tttgaaatgt gcggatggtc ggagtggaag
    26941 attgatacag ttttttggtt gatcagcatt gtgacttcgt tgatcacatt atggatcgtt
    27001 tggtaggaga tgacgggaaa tgaaaaaaat atcaacatat gaaaataaga aagtgcttgt
    27061 cttaggactc gcaaaaagcg gagtaagtgc ggccaaatta ttacatgaat tgggcgcact
    27121 tgtcaccgtt aatgatggaa aaccttttga cgaaaatccg gaagcacaag agctgctttc
    27181 tttagggata aaagtaataa caggtagtca tccaatcgaa ttgttggatg aagaattttc
    27241 attgatggtc aaaaatccag ggattcccta caggcatcct ttagtagcaa aagcccaaga
    27301 aatgggtatt ccggtaatta cggaagttga gctagcttac gaagtagcgg aatgcccaat
    27361 tattgggatt acaggaacga acggaaaaac aacgacaaca acgatgacag gactactact
    27421 gaatgcagga gcagatcaag gaattgcacg tttagcgggt aatattgggt atccagcaag
    27481 tggtgtagcg caagaagcaa aatcagaaga taaaattgtg atggaattgt ctagcttcca
    27541 actcatggga atcacagatt tccgccctca tatagcggtg attaccaata tctatgaagc
    27601 acatattgat tatcatggta caagaaaaga atatgtcaaa gctaaatgga accttcaaaa
    27661 aaacatgaca gaaaaagatt atctgatcct taactggaat cagtcagaac tacaagaatt
    27721 agcgcaaaga acaaaagcac gcgtactgcc tttctcgaca aaagaagtgt tggaagacgg
    27781 tgtgtatgct gacgattact caatatacta taaaaaagaa aaaataatgg aaatatctga
    27841 acttggcgta ccaggtaagc ataatgtaga aaatgcacta gctgctattt ctgtagcaaa
    27901 attgtatggt atatcaaatg aagctatacg agaaacgttg cattttttcc atggcgtacc
    27961 acatcgtacg caatatgtag gagaaatcca aggcagaaaa ttttataatg attcaaaagc
    28021 aaccaatatt ttagcgacta aaatggctct aagtggattt gaaacgtcta aggtcgtgtt
    28081 attggctggc ggattagaca gaggaaatac atttgatgaa ttaattcctt cattaaaagg
    28141 aatcaaagca atggtcgtat ttggccaaac aaaagaaaag ttgatggatg ctggaaaaaa
    28201 agcaggcatc gagacaatag taacagccga ttctgtagaa caagcagttc ctttagcatt
    28261 agaaaacagt acagatggtg atgtagtttt attatcacca gcgaatgcca gctgggatca
    28321 atacccaaat tttgaaacta gaggaaacag attcatggaa gctgtgaacc gattaaaata
    28381 gaaaaagtag aagcgagcga taaaaaatga aaattttggt tacaggcgga ggaacagggg
    28441 gacatattta tccagctctt gcctttgtca attatgtaaa gactcaagaa ccgaatgctg
    28501 aattcatgta tgtgggagca aaacgaggtc tagaaaacaa aatcgtccct gatacaggaa
    28561 tgcctttcca taccttggag attcaaggat ttaaacgaaa aatttccatg cataacatca
    28621 agacggtccg attgtttctt aagagtatcc gtcaagcaaa aaaaatcctc agtgattttc
    28681 aaccagatat cgttatcggt actggaggat atgtttccgg agcggtagtg tacgctgcag
    28741 ccaaaatggg tattccaaca gttatccatg aacaaaacag tgtaccaggt gttaccaata
    28801 aatttttaag ccgatatgtg gatcggatcg ctctttcatt taaagatgcg gcttcctttt
    28861 ttcctgaaaa caaagcagtt cttgtaggta atcctcgagc acaagaagta gcagatacta
    28921 aaaaatcaga tattttgaaa acatttgatt tggatccaga aaagaaaaca gtcttgattt
    28981 ttggcggtag tcaaggagca cttaaaatca atcaagcagt atcagttttt ttaagtgaat
    29041 atattccttc ttcttaccaa gttttgtatg cttcaggaga tagatattat gaagagatcc
    29101 taaataaaat aaatcctgaa atacaaaata ttagtgtcca gccttatatc aaaaatatgg
    29161 cagaggtcat ggcttccagt gatctgctgg tggggcgtgc tggtgcaact tcgatcgcag
    29221 aattcacagc tctggggtta ccggcagtgt tggttcccag cccatatgtt acgaatgatc
    29281 atcagactaa aaatgcaatg agtcttgttc acgcaggagc agcaaaaatg attgcagata
    29341 atgaactaac aggtgaatca ctaagtcaga cagtcaacga aatcatgggt gatgaagaat
    29401 tgcaaaagca aatgtgccga gcttctaaag aacaaggaat ccctgatgct tcaaaacgtc
    29461 tttatgactt agtaaaacaa ataatcaaaa aataaaggcg gtggtcttag tgagtaagaa
    29521 aaaagaacca aaaaaattgt ctcatccgtc ttctgatgaa aacaatttga ctccttggca
    29581 aaaggctaac cgaaaatatc tggcagaaca agagatgaaa aaccaagagc caaaaaaaga
    29641 agacataaca ttagatacac ccctttcaga ttctgaagaa gaaaaaacgg ttcaagagga
    29701 acagcaagaa gaaagccagt ccgagaaaaa aatcgaattt tttgaagaga tcaagcaaaa
    29761 gaaaaacggt ggaccatata acggttcttt tttgaaccgg ctgcccaatt taaaaagtca
    29821 acggcacaaa gtactatttc gtcggttagc tttgatcatc actgtattgg gtataccgct
    29881 gatttttttg atctattatg tttctcctta tagcaaatta caagcagttg cagtatcagg
    29941 caataagaca gtcaacagcc aagaaatcat ctctgataca aaattgtcat taggagaaaa
    30001 tgtttgggga cagtattttc atcgatctac ttatatcgat cgccttaaaa aagcacagcc
    30061 aagaatcgaa acagcaaata tacatttcaa agggatgaat gaattcgatt tggatgtaac
    30121 agaatataaa gagattgctt tgatcgcaaa aaacaatcaa tactacccag tgattgaaaa
    30181 tggaacagta ttagatgaaa aagtagcaaa tcctacaaaa aatctcccta tcttggaaga
    30241 gttcaaagac tctgcaaaaa tcaaagaatt aacaaagcaa tacaatcagt tatcatcaga
    30301 attacaaaaa gcaatttctg aaatcaagta cacgccaaga gcaagcaata aaaatctgat
    30361 ccaattgaat atgaatgacg gcaatcaagt tattgtaaat atcaataatc ttgcgaacca
    30421 aatgaaatat tattcgcaag tagccaaaga tatggatgaa aaaggtgtga tcgacatgga
    30481 agttggtatc ttctcgcatc cttacggtag cactcaagaa acggaagaat cgggccaatc
    30541 ggaagaaaca actgaaagtg aagaaaatgc ggatcaaaca aacgaatcaa gtgaagaggc
    30601 agaaacgcaa aaagcacaaa cacctggatc gtctagcaca aacggatcat ctgattcaaa
    30661 tgattcaagt gaaacaagtc aaatagaaga taatccaagt acaactccgt caagtttaaa
    30721 tgaaaattga taaaacaact ttaaaaagta agaatgtact ttctaaaaac cagtaattta
    30781 tggtagaatt ggaagcagtg aatatatcta ttaataagta tattttaaaa cggacgctag
    30841 ttgttcgctt gtatatggaa tagtaagtag gagggaatcc cattcatggc aaaaacagga
    30901 atgtatgtag gccttgatat tggaacgaca tctgttaaag ttgtcgtggc tgaatatatc
    30961 gacagccaaa tgaatattat tggagtagga aacgcaaaat cagaagggat taaccgaggc
    31021 attatcgttg atatcgataa aacggtccaa gcgatacaac gagcagtacg acaagcagaa
    31081 gaaaaagcag gtatccagat caaaggcgta agtgtcggtt tgccagcaaa catgcttgaa
    31141 gtagaaaatt gccagggaat gattgcagtt aacggagatt caaaggaaat caccgatgaa
    31201 gatgtcagaa atgtggcttc agctgccctt gttcgttcaa tacctcctga acgtcaaatc
    31261 gtgtcaatat tgccacaaga ttttactgtc gatgggttcg agggaatcaa agatcctcgt
    31321 ggaatgatcg gcgtaagatt agaaatgtac ggtctgttgt ttacaggacc taaaacgatc
    31381 atccataaca tacgtaaatg cgtggaaaat gccggattaa tcgtcaacga aatggtgatt
    31441 actcctttag cgctgactga atcaattctt tctgacggag aaaaagattt tggtacgatc
    31501 gtcattgata tgggtggcgg ccaaacaaca acggctgtta tgcatgataa gcaattgaag
    31561 tttaccaatc ttgaccaaga aggtggcgaa tttgtcacta aagatatctc tattgtatta
    31621 aatacgtcat ttaacaatgc agaagcgttg aaaataaact acggggatgc atacccagaa
    31681 cggacatcac cagatgaaga atttccagta gatgtgattg gtcagtcaga accagtgaaa
    31741 gtcgatgaac gctacttgtc agaaattatt tcagctcgaa tggaacagat tttcaacaaa
    31801 gcaaaagatg cattggacca aatcgaagca ttagagttgc caggtggcgt cgtattgaca
    31861 ggcggagctg ctagtcttcc tggtgttgtg gacttggcac aagaaatctt cggcgtaaat
    31921 gtaaaattgt atgttccaaa tcacatgggg ctgagaaacc cggtatttac aaatgtaata
    31981 agtatcgtgg attattcagc aaacttgagt gaagtgtatc aattggcaaa aattgctgtt
    32041 acgggtgaaa cctcagcagc tcgtcaaatg gtagttgaac aagaaacaac aaacacatat
    32101 gagagctatg aagcacctga agaaacaatc tacgatgagc cggaaccaaa agagtccgga
    32161 gaaaatgtaa aaaataaaat caagggtttc tttacaaata tttttgatta attcatcctg
    32221 ggaggaataa gatatggaat tctcaattga taataatatc aatgacggcg cagtaatcaa
    32281 agtcatcgga gtcggtggtg gcggtggaaa tgctgtaaac cgaatgattg aagaaaacgt
    32341 taaaggtgtt gaattcatta cagccaacac agacgtgcaa gcattaaaaa attcaaaagc
    32401 tgaaacagtt atccaattag gccccaaata tactcgtggt ttaggtgctg gttctcagcc
    32461 agaagttgga caaaaagcgg cagaagaaag tgaacaatcc ttacgcgaag ctttagacgg
    32521 tgcagatatg attttcatca ctgcaggaat gggtggagga accggtacag gtgctgcacc
    32581 aatcgttgca ggtatcgcga aagaattggg cgcattgact gttggtgtcg tgacacgtcc
    32641 attcactttt gaaggaccga aacgtggccg ttttgctgca gaaggtatcg ctcgtttgaa
    32701 agaaaatgtg gatacattat taattatctc aaataaccgt ttattagaag ttgtagataa
    32761 aaagacaccg atgcttgaag ctttccgtga agcagataat gttttacgac aaggtgtaca
    32821 aggtatctct gatttgatca cagcgccagg ttacgttaac cttgactttg ctgacgtgaa
    32881 aacagtgatg gaaaaccaag gtacagcatt gatggggatc ggtgtggcta gtggagaaga
    32941 tcgcgtagta gaagcaacga aaaaagcaat ctcctctcca ttgcttgaaa catctattga
    33001 tggagctgaa caagtacttc ttaacatcac aggcggtttg gatatgacat tgttcgaagc
    33061 acaagatgct tcagatatcg tagcaaatgc agcaactggc gatgtaaata tcattctagg
    33121 tacatctatc aatgaagaaa tgggcgatga aatccgtgtg acggtcatcg caacaggtat
    33181 cgatgaatca aaaaaggaac gcaagtctag ccgaccagct agacaagcac aaatgcaatc
    33241 acctgctcaa aagactgtat tagatatgga tcaagctaaa ccaacttctt cagaagaaga
    33301 aaacagtttt ggcgattggg atatcagacg tgaacaaaat gttcgtccta gagttgatga
    33361 ttccaatttt gacaatatcg aaaaaaaaga atttgataca ttcaaccgcg aagaaacaaa
    33421 atctcatggc gatgatgaat taaatacgcc accgtttttc cgtcgtaaaa aataaaggag
    33481 aggaagagca catgattgct gacaacttgc agaaaatcaa gcaagaaatt caggattcgt
    33541 gtgctcttgt tcatcgtgat ccgaaagaag ttacattagt agctgtaaca aaatctgttg
    33601 aatccgctca agctgaagaa ttggttagag ctggcgttat gaattgtgca gaaaaccgag
    33661 tagataaatt attagaaaag aaacaagatt tatttgattt tcctgaaata aagtggcatt
    33721 taattggtaa tttacagcgc agaaaggtaa aattgattgt aaatgaaatt gattactttc
    33781 atgcgttgga cagtttaaga ttagcagaag aaatccagaa gcggttagag aaagaattat
    33841 cctgttttat tgaagtaaat gtttctggtg aggaatcaaa acatggaatt tcaccagatg
    33901 agcttttacc gtttgtggaa tctttagctg cctgcacaaa aatcaaggtt gttgggttga
    33961 tgactatggc gcctaaagaa gcttctgatg agcaaatcca taaagtgttt ggtacgctga
    34021 aagaattgcg tgatcttgtt caagcaaaag gttatgccca tgcaccatgt agtgaattaa
    34081 gtatggggat gagtcaagac tttcaaattg cgattgaaga aggtgcaaca tttatccgtg
    34141 taggaacagc gctattcaaa gacgaataaa ggaggaacaa aaatgccgct tttaaacaga
    34201 ggagcaattg ctaatttttt cggattagct gatgaggaag agtacgaata ccaagattat
    34261 cctgaaacaa caaaaaaggc tgtacagcca actaaaaaaa atacaacaca gaatcaatca
    34321 actcattctg tatctgctgc gaatactcat gaaaaacaaa aaacggcgac ttcacgtcct
    34381 gtaacgcatg aaagtattca gccggctgaa aagaaaaaag taattgaact acatcaatca
    34441 cctacaaaaa ctgtagcgaa agaaagaaca gcttcaagaa cctctacaca acctaaagca
    34501 aatcttgctg gaaaaatcac tgtaatggaa ccaagagctt attctgaagc tatggcgatt
    34561 gctaaacgaa tcattgctgg agaagcaacc ttagtgaatt ttcatttagt agaagaaaaa
    34621 caagcgagaa gaatcgttga ttttctaaca ggaacagttt atgcactaga tggagatatc
    34681 aaacgtgttg gagatgagat ttttctttgt acaccgactg ggatggagat tgacagttca
    34741 accgctcaat cttttgcaga tgcgaatttg tttgatctgt aagaggagga actacgattt
    34801 atactattgc actattactt catttacttc aacgggcagc ctatttctat agcattatct
    34861 tgatggtata tgcattgctc tcttggtttc caggcgggta tcaatcaggg ctaggacgat
    34921 tattagctaa gatctgcgaa ccgtatatca gtttgttcga tcgtctgcct ttgaggattg
    34981 gacccttgga tttcacaatt ttaggggcaa tcctgatctt gaacttagcc gtacaaggtt
    35041 tagcacagat cgttatatgg ataactgtcc atttagttta ttgattgaag caacaaaatc
    35101 tttattctca gaatagatag gaggggaaaa ttttggatgc aaatgtatac cagcactttc
    35161 gggcagatga acgtacattt atcgattcgg taggcgattg gattgaacag gttcaaggac
    35221 aatatgcacc ctatctgacc gaatttcttg acccaagaca atcctatatt ttagaaacac
    35281 tgattcgaca aagcagtgat ttgcgtttca tgttttatgg cggatatgaa caggcagaaa
    35341 gaaaaagatg tttgattttc cccgattatt acgaacctgt agaggaagat tttgaaacag
    35401 cattgtatga aatccattat cctagcaaat ttgccagcct aagtcatggg aaaatccttg
    35461 gaactttagt gggaacgggg atcaaacgct cttactttgg cgatatcatc tcggacggtg
    35521 atcgctggca agtatttatt gcaaatgagg tggatcattt tgttgctgca caagtaacaa
    35581 agattggaaa ggttgctgta cgcttggaac caatcaaata cacagaaatg attatgccga
    35641 aagatagctg gacacaagaa agaggaacag ttagttccat gcgactagac agtgtgatct
    35701 ctgagattta taatatttcc agacagcggt ccaagcagct gatcgaagct ggtaaagtaa
    35761 aagtgaactg gacagaaaat acaagacctg attttgtact tgaactatta gatattattt
    35821 cagtcagagg ttatggcaga gtccagatcc aagaacttga ggggaagacg aaaaaagaaa
    35881 agtacagggt actgttcggt gtattacgta aataaaataa aaaggtggtt tactcatggc
    35941 tttaactcca ttagatatcc aaaataagac attcccgaca aaaatgcggg gatataatca
    36001 agacgaagta gatgatttct tagatttagt tgttcgtgac tatgaggaat tgactcaaag
    36061 aaatcgagaa ttagaaaaag cagtcaaaca ttctgaagaa aaacttgagt acttcaatga
    36121 attaaaagat gctttgaacc aatcaatcat cgtagctcaa gatacggctg ataaagtaaa
    36181 aacaagtgca agcaaggaat ccgaagtgat cgttacttct gcacaaaaca aagcagatga
    36241 gttagttgcg aacgctgaaa aacgtgcaca tcaattaaca actgatgcag aagaaaaagc
    36301 aagaaaaatc ttgacagatg caacagaaaa agcaagacag cttgcgacag aaactgaaga
    36361 tcttaagaaa aaaacacgag tattccatca acgtatcagc ttgatgcttg agtctcaatt
    36421 agaacaagtg aaaagtccgg aatgggatga aattttacaa ccattctcca gctacgtgac
    36481 tgacagccat gaagtgatca aagaagtttt agcaaaacag cttgacaatg aaaatgatac
    36541 tgatgtaaac tcagacacag gagtcataga agcacctatg acggcatttg atacacaggc
    36601 tattgatatc tcgatcgtgc ctggtgaaaa tgaaaacgag tagaacagaa aagcactttg
    36661 ttattcttaa agcgagccga tgagagtgga agttcggtaa gacactgcat ggtgaaagat
    36721 cctctacttg agacctgctt ttccgaaatt agagtaagaa gcagcggctg acctcgttat
    36781 tggtcacaga gagtttgcac tagatagatg caactaaatt tgggtggtac cacggcatgt
    36841 tcgtcccttg cggctgaaca tgcttttttg tgtccggaaa acaaataaag gagtgtatgg
    36901 ttgaatgaaa atgaaagaaa ccttgcaatt aggaaaaaca aaattcccta tgcgtggtaa
    36961 tttgccaaac cgagaaggcg agtggcaaaa agaatgggaa gaaaatcgaa tctaccaaaa
    37021 aagacaagag ttgaacgaag gaaaacctag ctttgttctt catgatggac ctccatatgc
    37081 caatggtaat atccatttag gacattcatt gaataaaatc agtaaagaca ttatcattcg
    37141 ttcaaaatca atgtctggct atcgtgcacc atatgttcca ggatgggata cacatggatt
    37201 gccgattgaa caagtattgg ctaataaagg cgtgaaacgt aaagaaatgt ccatggcaga
    37261 atacttggaa aaatgtcgtg aatatgcact ttcccaagta gataagcaac gagcagattt
    37321 caaacgccta ggagttgcag gagattggga acatccttat gttacattag atccttctta
    37381 tgaagctgca caagtacgcg tatttggtaa aatggctgaa aaaagctata tttacaaagg
    37441 attgaaacca atttactggt ctccgtcaag tgagtcttct ttagcggaag ctgaaattga
    37501 atataaagat gtaaaatctc catctatttt tgtagctttc aatgtcaaag acggcaaaga
    37561 tattcttgat gaagatacag cttttgtcat ttggacgaca acaccatgga cattgccagc
    37621 aaaccaaggt attgctgtaa atccaaaatt tacgtatgta ttggtggaag ccgatggccg
    37681 taagtttgtc gtagcgaaag atttgctaga aacagtccaa tcagaaatcg gttgggaaaa
    37741 tgtaacagtt ctaaaagaat ttgctggaca agaaatggaa tatatgactg caagtcatcc
    37801 attctatgaa cgtgaatcac ttgtgatttt aggcgatcat gttactttag acgctggtac
    37861 tggattagtc catactgctc caggacatgg ggaagatgac tatatcgctg gtagcaaata
    37921 taaactatct gtagtgtcac ctgtagactc aaaaggattg tttacagaag aagcacctgg
    37981 atttgaaggt atcttttatg acaaagcaaa tcctatgatc actgatctat taaaagaaaa
    38041 aggagctttg ctgaagctag atttctttac tcatagttat cctcacgatt ggcggacgaa
    38101 aaagcctgtg atctatcggg caactccaca atggtttgca tctatcgata aattccgtca
    38161 gaatattttg gatgaagtag aaaaagtaga gtgggttatc ccatggggta aaacacgttt
    38221 gtataatatg atccgtgacc gcggcgattg ggtaatttcc cgtcagcgtg catggggagt
    38281 accacttccg attttctatg ctgaaaatgg agaagcaatc atcacaccag aaacaatcga
    38341 acatgtggca aaattatttg ctgaacatgg atcaattatc tggtttgaaa aagaagcgaa
    38401 agaacttctt cctgaaggat ttacccaccc aggttctcct aatggcgaat ttacaaaaga
    38461 aaaagatatc atggatgtct ggtttgattc tggttcttct catgaagctg ttttacgtca
    38521 gcgaccagaa ttatcattcc cagcagatat gtatttagaa ggatcagacc aatatcgtgg
    38581 ctggttcaac tcaagtatca cgactagtgt ggctatcaat ggtgtagcac catacaaatc
    38641 agttatttct caaggatttg ctttagatgg tgaaggccgt aagatgagta aatctttagg
    38701 aaacatcatt gcaccagaaa aagtgatcaa acaatttggt gcagatatcc ttcgcttatg
    38761 ggtatctagt gtagattatg aagcagatgt tcgtgtttct atggatatct taagtcaagt
    38821 ggcagaagta tatcgtaaaa tcagaaatac gatgcgtttc ttattagcga atacagaaga
    38881 tttcgatcct aaaaaagata ctgtttcgta caatgattta cgttctgtag acaaatatat
    38941 gaccgttcga ttgaaccaag taatcaaaga aatccgagaa gaagggtacg acaaatataa
    39001 cttcatgcat atttaccgta cagttatgaa cttcttgacg gttgatctat cgtcattcta
    39061 cctggatttt gctaaagatg tagtttatat tgaagcagaa gatgattatc aacgtcgttg
    39121 catgcaaact gttttctatc aaacagctgt agctttgaca aaattattga ctcctatcat
    39181 tccgcataca gcagaagaaa tctggtcatt cctgaaagaa gaagaggagt acgttcagtt
    39241 aagcgagttt cctggttatg aagagtttgc aaaccaagaa gaattgatgg atacctggac
    39301 tgctttcatg gacttccgtg ataatgtctt aaaagcattg gaagaagcaa gaaacagcaa
    39361 attgattggt aaatctttgg aagcaaaact aactgtttat ccaaaacaac aagttcgtga
    39421 aatgttaaaa gctttggatg cagatattgc acaattattg atcgtttcac cagactactt
    39481 tgaagttatg gcagcagatg aacaagtgcc agataacgct atggtatttg atgatgttgc
    39541 tattacagtg gaaaaagcag acggggaaac ttgcgatcgc tgtcgccaag ttcgtaaaga
    39601 tgtaggagta gatgaaaaac ttcctcatct atgcggtcgt tgtgcaaaaa tcgttgaaga
    39661 cttctatcct gaagctgtag ctgaaggatt cgaagaaaag taaaagggaa aagggggtgt
    39721 gacatgttct ttgtcacacc cccttttgtt atcaatcgtt taattttcca cgttcacgat
    39781 accagttatc tggttgccag atccattcga atccgtcttt ttcaagtagt tcgaaagctg
    39841 ctttaggtcc cattgatcca gctttgtagt tgggaaaatc cggttgtgtt tggtcccaag
    39901 cttttcgaat gacatccacg atatgccaag attgagcgac ttcttcccaa tgagagaagt
    39961 tcgttccatc accattcaac gcatctaata gtaatttttc gtaagcttct gggctatttt
    40021 caaccatttc tgcactattt ctaaattcca atttgattgg ttccgtttca aatccttgcc
    40081 ctacttcttt cccattcaag gataaagaaa atccttcagt tggctgaata tagatcgtta
    40141 gaacattagg tggaagtgtc gtatcgtcac atggttcatc gacagatgtt ttgaatacat
    40201 taacaggaac ttgtttgaat acgatgttga tccgagtccc tttttcagtc aaacgtttgc
    40261 ctgtacgtac atagaaagga acacctgacc aacggaagtt atcgatagtg aatttgcctg
    40321 cgacgaatgt ttcagtctct gattgagcat ccactttgtc ttcttcgcga tagcccttga
    40381 attgtacacc atccaattca ccttgagcat attgcccgcg aacgaagttt tctaacactt
    40441 cttcttcgtt ataaatgcgg atagctttca atgctttgat cttttctgta cggatttctt
    40501 tttcggaaaa tgctactggc ggttccattg caagtaatgc tacaacctgt aaaatatgat
    40561 tttgcaccat gtctttaagt gcgccactat gatcgtagta gcccccgcga tcttctacac
    40621 cgaggttttc gccaaaagta atttggatat tgtcgatata gcgattgttc cattgagcct
    40681 caaaaatatt attggcaaaa cggatagctg agatgttttg gatcatttct tttcctaaat
    40741 aatgatcaat cctgaaaata tcttgttctg ggaatacttc tctgatttgc tggttcaatt
    40801 caaaagcaga ctggtaatca gaaccgaacg gtttttctat gatcaaacga ttgaagccat
    40861 tttttgtcat aatattttgt gatttcaaat gagaaacgat cgttccaaaa aattgaggtg
    40921 ccatagccaa ataatagaca cggttaccgt ttaattgata gtgtccatct aatcggtcag
    40981 acaattcttt taatgtctga taatgcgttg aatcatttac atcatgggat tgatagtaaa
    41041 aatggcttgc aaaggtagtt gcttcttcat ctgaaggatt caatgcacgg attgtttctt
    41101 tgacaacttc acggtagtgc tcatcgctcc agggacggcg agcggttccg attacagcaa
    41161 aatgctcgcc taaatttcct tttctgtaaa ggcggaacaa agacgggtac aatttacgct
    41221 gggctaggtc acctgtaccg ccgaatatcg taaacaatac atttttttca ttagacatgt
    41281 ggatacactc ctttatattt tatcgacaaa aatcgtgctg gccgccttaa agctgacggg
    41341 gattttcttt ctatcatttt ggatagtgat cggtccttca taagctgcga tgtcaacgat
    41401 ctcatattct tcgttgatat tcaggtcaat agtgactaag taatccaaca attctttttc
    41461 atcaagcact cgtgcgatac gcacttttgt accgacagga taggactcca atgtttgtcg
    41521 gcgttcttca tggacaactt cctctttttt aggaatcgat cctccatgag gacaatgttc
    41581 aggatgattc aaatagtttt ctaaatgatc tgcaagatgt tccgaagtca catgttccaa
    41641 tacttcagca tcatcatgga cttcattcca gctatagtct aaatgttcaa ctaaaaaaac
    41701 ttcccataag cgatgtttgc gaatcagact gctagctttt tgcagtccgc ttgcagtcaa
    41761 ttgtacacct tgataagggg aatgttctac aagctcttct ttcacaagtt tagaaatcat
    41821 ttcactgaca gatgcagctg agacatctag actagatacg atttgtttat tgttgacttt
    41881 cgttgtatct ccacctagtt ctaaaatgat tttcaaataa tcttctcgat ttggtgtcat
    41941 attcgatcat cctttcatgg taaaaaacca tcattagtaa gtgtaacaaa aaaaacgtta
    42001 attgactatt tttctaaata aaaaacaaag gatttttaga cgtttttaca acaaaaaacg
    42061 aaagatgata gagaagactc attctttcgt ttataaagga tatatatata cattgggagg
    42121 gatctatttt tcagataaca acttatatga agtgtctgca gattcgtcat aaagagtttg
    42181 ttcgggataa gaaaagataa gataaaaacc atactgatca cgtacgacta aacgatattc
    42241 atctatttct accacagatg tgccaagaga ctgaccgttg atcaaaaacg tgtaatcaga
    42301 tgtaatctgt aattgttgtt tttgctggct atcactaaag aaccatgtac cttgatactt
    42361 tgagagtcct tctgactttt ccgcttgttt tttctttttc aagtaaataa aacttgagag
    42421 aacggtaatg agtgaaccaa gaaaatagat catttttttt atcaccaaat cactccttta
    42481 tgagaaaata atcgcataga catattcttt cttctttatt tcattgtacc gaataaaagc
    42541 taaaatttct ctaagacttt cgactggttt ttttactgtt tttctacaaa ttatgatagg
    42601 atacatagta ggaagaaagg tgacataatt tgaagtatca gcaagtggaa atagcttttt
    42661 ttattgaccg cccgaatcgg tttatagcac attgtatgaa aaaagacgga gaaataatta
    42721 ttgcgcatgt caaaaataca ggtagaggaa aagaggtcct gataccaggc gctgaagtgg
    42781 ctattgtatt tgctcctgga cctaaacgga aaaccgccta tgatctgatt gctgtaaaaa
    42841 agaagggtga ctggtttaat atcgatagtc aattgcctaa tcggctggca attgatggta
    42901 ttttagacgg cacgatcctt ttaccaggat taactggtcc atttacggta tacaagcgtg
    42961 aagtaactta tcttcactcc aaatttgata tttatttaga gacggtggtc ggacaaaaag
    43021 catttgttga agtcaaaggt atgacacttg aaaataaagc gattggtgct tttcctgatg
    43081 cgcccacact cagaggattg aaacatatag gagaattaac gtatgccgca caagatggtt
    43141 atgccgctta tgtcttgttt attgctcagt tcgaacatct tcatctggca acgatccatg
    43201 aagaaatgca accagcacta gctgatatgg ttcgacatgc gcagcaaagc ggagttcaaa
    43261 tcctcgccta taattgtcaa gtatcggcag atgaggtaac tgtcaagcag gccattccat
    43321 ttgatgtaga tatccctttt gaagatccta tacaatagaa agaaggtaaa ataatgatca
    43381 acttaggtat tatcggaaca aattggatta ctcatcaatt tgtccaagca gctttaagca
    43441 caaaaaaata tgaattgaca gcggtatatt ctagacattt ggaaaaagca caggaatttg
    43501 gttctcatta tgaaggggat atcgaattcg caacagattt gaagactttt ttcaatatta
    43561 ctcatatgga caccatttat attgcttctc cgaatagtct tcactttagt caagctaagc
    43621 aagcaatttt ggcaggaaaa aatgtgatag ttgaaaagcc agctttttcg acgccaaaag
    43681 aaatggatga aatcatccat ttggcaaatg aacacaatgt gtattttttt gaagcagcaa
    43741 gaaatatcca tgaatcaggt tttaaaaaga ttgccgatta cttaccatta aaagacgaga
    43801 taatcggtgc aaatttcagc tatatgaaat attcttcgcg ctatgaccaa gtattagatg
    43861 gagaagaacc gaatatcttc tcgactcatt tctcaggtgg tgcattgatg gatttaggtg
    43921 tttaccttgt atatgcagct gttgcttggt ttggaatgcc aaaagaagtc cattatttcc
    43981 caagaaagat ttctactggt gtagatggtc ttggatgggg gatcttacgt tatgagaatt
    44041 ttgatgtatc gatccagcca ggtaaaatcg gtgattctta tttaccgtca gagatttatt
    44101 ttgactcagg aacgctagtg atgaatggag tcaatgcaat tgaacaagca caatttcatg
    44161 atcgcgcgca taaagaaatc attgatttgg cggtaacagc tgcagaaaat ccaatggcag
    44221 atgaagcaga ggattttgcc aatgtcatgg ctcatccgaa agatccagct tggggattgt
    44281 tgtatgaaga gtggatcgag ctagctagaa atgtgaacca agtgatttat gatttaagaa
    44341 aaaatggcgg catcaaattc gatgcagata atgaggaaga tttttaatga acgaatttgt
    44401 acaagtattt cccgaaaatt ggcagaaaaa atatgaggat aaagggttta tctcaccttc
    44461 tcttattcaa cgagcagttt ttcagccact aagtaataaa caaagtatag tagcggtttc
    44521 tcctacaggt tcaggtaaaa cactggctta tctttggcct ctactactaa acgtggaacc
    44581 aggtgaagca agtgcgttgg tcatttttgc ttcttctcaa gaactagcta ttcaagtagc
    44641 agatgttgca cgtgaatggg gaaaagacaa agagttgaaa gtccaatctt tggtgggcgg
    44701 agccaacgtc aaacgtcaga ttgagggttt gaaggaaaaa ccagaaatct tgattggtac
    44761 accaggaaga atattggaat tgatgaaagc aaaaaaaata aaagcacatc aagtgaaaac
    44821 aatggtattt gatgaagcag accagctatt tgacgctggg aacagccaaa tcattgatca
    44881 aattcttcat caagctccaa cagagtatca gctagctttt ttttcagcga cagcagatcg
    44941 ttcccttgaa agcattgaaa aaatcactgg gaaaacgttt gagacaattg acgtgacagc
    45001 agaggacgat tctcgaaaag gactgagaca ttacttttta cgtgtaccga tacgaaagaa
    45061 agatgactac ttgagacgtc tcacgcatat agaagatttt catggtctga ttttctttaa
    45121 tcaactgact gaattaggcg taatggaaga aaaattgctg tatagaggtg taccggtagg
    45181 aagtctggcc tctgatcaga acaagctact ccgaaaagca gcgctagaac aattcaaaaa
    45241 gaaaatggtc agagcacttt tgaccacaga tgtggcggct agaggtttag atattacagg
    45301 tcttccttat gtagtgaatg cagaagttcc gttaagtgaa gaagcttatt tgcatcgttc
    45361 gggaagaact ggtcgaatgg gtaatgaagg aatagtaatt acactcgtac aagataataa
    45421 tctaagagat ttaaaacggc ttttacgtcc atcaaatata acacttgaag agattttttt
    45481 atacggaggt aagctgcaaa ctgagcagcc aaccaaagaa gatgcagctc ctgcagtaaa
    45541 taaaaagtat gcgaaaactt tttctggaaa ggagcagaac ccagaatata acaaagaaaa
    45601 aataagaaaa aaagttaaaa ataaatcaaa aaaagagaga aataaaggag caaggcgcaa
    45661 ataagcgcct ttttatgcta tgattatgaa aacgggaaca aaattggttg ccagaggagg
    45721 ggttatgtga aagaacgtca agaagagatc ttgcagctat tgacagaaaa ccaacaagga
    45781 ttgacagcaa gtgatgtagc tgagcgctta actatcgatc gaagcaatgc aagccgatat
    45841 ttaagtgaac tatacaaggc ccatcatatt gtaaaaaccg ctggccgacc agtcgtttac
    45901 tctttgccaa cggaaaagtc aaaatctgat gaagtgcatg tcgactcttc tacgcaagtg
    45961 acttttgaaa cactcgttgg agaaaatgat tctttaaaag tcagcataca gcaggcaaaa
    46021 gctgcgattc tttatccacc gcgggggttg catacgatta tttttggaga aactggtaca
    46081 gggaagtcga tgtttgctga atgtatgtat cattttgcaa tcgattcaga gatgctttct
    46141 gcagatgcgc catttgtttc tttcaattgt gcggattatg ctcaaaatcc gcaactgctc
    46201 tttggacaca tctttgggat caaaaaaggg gcttacacag gagcagctca agatagtccc
    46261 ggtctaatag ccaaagcaga cggcggtata ttgtttttag atgagatcca ccgtttaccg
    46321 ccggaagggc aggaaatgct gtttactttc attgataaag gtatttaccg tccgcttgga
    46381 gaaagtgcac aagtacatga agcatctgtt cagatcattg gggcgacgac tgaatcttca
    46441 gaaagtttct tatctacgtt caatcgacgg attcctatgg caatcacctt gccgaatctt
    46501 gcttcacgtt cactggatga gcgttatgaa atcatctctc tttttatcaa acaagaagcg
    46561 aatcgtttga atcaacgtat cgatatcgaa aaagaagcga ttcttgcctt catgctttat
    46621 gatgcggaag gaaatatcgg tcaagtgaaa cgagacttga aactagtctg tgcaaaatct
    46681 tttttacatt accgtactca tcatgagaaa aagctaatca ttcgaaaaga agaactttcc
    46741 cttcaagtac aaaaaggatt actgaaaatc aaggaaatac cggaacgtct cgaccgattc
    46801 atggacagca aaagtcagta tttgacattt gaaccaggat ttgctgatgt tgtctggtca
    46861 caagatccag aacgaaacat gcaggtctat aacgatattg aggaaaaagt cacttctctt
    46921 tccgaaaccg gtgtggagaa cattgatcta gagaccctca tttcaaaaga tgtggatgct
    46981 tatttccaga catatgtcaa agaattgact aaaagttcga ttcccaaaga tcttttgcca
    47041 gatgaaatct ggcggttaac caatcaattg tatgatgtgg ctgaaaaacg gttagaccgt
    47101 atttataatg aaaaagctcg ttttgctttt gctttgcatc tgcaaagtac attggatcgt
    47161 gtgagagaag gtcatatgat cgtccaccca gatttgaata acgtacggaa aaattttaag
    47221 agggaattcc aggtagcaat cgatctttca acgatcattg aagaagaaga acaagttgag
    47281 attccttttg atgaaatcgg ctttattagt atgtttcttt ctatcaatct aggagaaagt
    47341 gagcctgtta aagaaaacaa agtagatgtt ctcatattga tgcacggcag acagactgct
    47401 tcaagcatgt tggagatggc acaagaacta ttaggaacaa aagtagggaa agcttttaat
    47461 atgccactgg ttatggaagt tcaaacaatg tatgaagaag tgttacaata tgtcaaaaac
    47521 tatcaagagc atttgacgaa tggcctactc ttgttaacag atatgggatc attaaacacc
    47581 ttcagtgatt tgatttatga agagactggc attcggacga aagctatcac gatgacaagt
    47641 accatgattg tgatagaagc gatacggatg gcaagtgttg gaagaagctt agaagatatt
    47701 tatcaaaata tccagctttc ctttgaatcg atcgtacgtg atcagttcaa agcggaaaag
    47761 tctgaaactt tacagcgtaa aaaagccgta atcgtgacat gctttactgg tgaaggagta
    47821 gctgccaaat tatatcagcg tattatgcca gtgattaatc aaaataaggt agaactgatc
    47881 cagatgcagt ttttagaacg tgaatcattc aaaaaacaca tcgatgcctt attggaagaa
    47941 tatgagatca aagcaattgc gggaacagtt gatgttgatt atcaaaatat cccatttttt
    48001 tcagcctatg acgtttttga tgatgaaaaa ctaaatattt taaagcgtat agctgatgat
    48061 gagatcccga cagctaaaat tgtcgattca cttgctggat cattaagcga tgtcttttct
    48121 attgatgaac tgatccgttc attgcaaaag acagttcagc agatccagac cgatttgtcg
    48181 attatcgtgg aacctggagt agatactgga atcgttatcc atttagcctt catgttagac
    48241 ggattgatca aacaagaaag ccatcggcaa tttgagaaac tgtctatttt ctcaaaaatt
    48301 tatcgtttgg aaatggatat cgttcgggca aacttgatgg cactagaacg aaaatatcag
    48361 gtaaaaattc cagaagatga tatcgcattt cttacacaaa tgtttcttga aaatagaata
    48421 aaaaaatgaa cacactaatc atactgttta cacactaaac atctgtgtgc gcactataaa
    48481 aaagagctaa aaagcatgaa aacgctattt cagctctttt ttttattggc atgcttcttg
    48541 cgttatataa aagtgtaaaa aaataaaagg agtgttaaaa atggcaaaaa aaacaatcat
    48601 gttagtttgt tcagcaggga tgagtacgag tttattggtc acaaaaatgc aaaaagcagc
    48661 agaagaaaaa gggatggaag ccgatatttt tgcggtctct gcctcagatg cagataataa
    48721 tttagaatct aagaatgtcg atgtattatt attaggtcct caagtacgtt tcatgaaagc
    48781 tcaatttgaa caaaaacttg cgcctaaagg tattccatta gatgtcatca atatgcaaga
    48841 ctatggcatg atgaacggag aaaaagttct tgcacaagca gaaaatctaa tgaaataaat
    48901 caagtatgca gttgggggta taacaacgtg gaagatcaac aaaatctaga agccatcatg
    48961 gggttaatca tgtttggcgg taatgcaaag agcgatgcaa tggaagcgat tgcggctgct
    49021 aaaatcggtg attttgagtt agcggatagg aaaatcgctg atgcagaaga atcacttata
    49081 caagcgcatc atgcacaaac agacatgttg acacaagaag cacaaggaaa tcatatgcaa
    49141 gtgacattat tgactgttca cagtcaagat cacttaatga catctattgc gtttactgat
    49201 ttagcaaaag aaatcatcga actttatcgt cgcgtataca gcgaataaat acaacttctt
    49261 ttttgaacgg aagaagaaaa atttttgaac ggatttttta ttttgttata aggagaactc
    49321 tctttatata aaaaacttat aggaggaagt aaaaaatgaa cggactatca agttggatgg
    49381 agaaatacat cctgccggtt gccgcaaaaa tcggagcgca aaaacattta gttgctttgc
    49441 gtgatgcttt tatcggcaca ctgcccgcaa caatggcggg atcagtcgct gtaatgatca
    49501 atgcaattat tcgtgattta ccgcaacagt ttaacagtac ctatgctgac agtttagcag
    49561 cagacaaagg aatctttgcc gtattgaatg tcatcatcgg aatcaatggt tttgtctgga
    49621 acggtacact agcaatcgcc ggattgatct ttgccttttc ttgggggtat aatcttgccc
    49681 gagcttatgg agtaaatgat ctagctgggg ggatcgttgg tttagctacc ttgattcaag
    49741 gtatttcctt tggtccagca ttatcagcag cattagatgt gaatgttcct tcaaatgtcg
    49801 cagatgcaat caatagttct gagattggtg caacgattgc agatggcaca ctgtcagttg
    49861 gattatgggg ttggctaaaa ctagatcatt tgaatggaaa cgcttatttt acagttatga
    49921 tcatgggagc tatttcagtc attatttttt gtaaattgat gcttgccaac attacgatca
    49981 aaatgcctga ttcagtacca ccagcagtat ctaaagcatt tgcagccatt ttaccagcga
    50041 cgatcgcttt atacgtcatt gctattatca attatatcgt aggtcgtgta gctgatgggc
    50101 aactgatcat tgatttggtg caaaaatata ttgcagaacc attcctagga ttatctcaag
    50161 ggttgggagc agtattgatc gtcacagtat ttgttcaagt attctggttc tttggtattc
    50221 atggaccaaa tgtattggca ccagtgctag agggaatctg gggacaagca caactaatca
    50281 atattgatat tttccaaaaa ggttataacg gaaaaactgg tacaccagcc gtgttagatg
    50341 caatcgaaga tggaaaagct tatatgtggg tccgcggatc gttcgatgct tttgcatggt
    50401 ttggcggatc aggcggaacg atcgtcttac tgatagctat cttgattttc tctaaacgtg
    50461 cagattatct aacagtagct aaattatcca ttgggcctgg tatcttcaat atcaacgaac
    50521 cagtgatgtt tggtctgcca atcgtattga atgcaatcat gtttatccca ttcttgattg
    50581 ctccagtagt agcaacagca attggatgga cagctactta tcttggccta gtcgctccgg
    50641 tatcgcagca agtcacatgg gtagtaccgc cgttcttatt gtcattctta gcaacaggag
    50701 cggattggcg ggcaccgatc gtaacattag tatgtatggt cgtgactttc ttgatttggg
    50761 caccatttgt catctctgca aacaaaatgg accctgccgc aggcgaacaa caataagatg
    50821 aacgtgagga gcgggacgct ttaagtttcg ttcctcataa tgaattttcg aggaggaact
    50881 gaaatgatta caattgccag tgcaatgcaa caagatgaaa tcaaagaatt gttagaagga
    50941 tacaatgaag aaggaatgac ttttacattt aaagaaaaac aaggaatcaa gctgttcttt
    51001 gaaacaagtg cagaagatcc ggaagttgcg gctaaaaaag caaaggaact aatcaaagca
    51061 cagccttggg gaacagtcct ttattttcaa tcaacagctg tttagaaaat ggtttaaaaa
    51121 gataggatgg ttgagatgat aaaaaacggt ttatttttat gtagtattgg ttctttgatc
    51181 gtgttgtata gtttgaatcc acattcgcaa aggtatgatt tgtatagtat ggtcacaggt
    51241 ctttttctaa caggattagg aatctatttc ttcatgaagg ggaaaaaaag agaagaagca
    51301 aaggagaaaa ataactaatg gaaatcacga aaaaattaaa tattccagca tcgttttttt
    51361 acgataaagt tatggactct gtccttttcg atgtacgtaa agcgaccggc aaatcagtaa
    51421 ctagaaaaca gctgaaaaat ttggagtacg tcaaacaatt ctcgcaaaat aaccgggctc
    51481 gcataaaaat cgaagaagtc attgaaaacc agtcttataa gtttcgtaca tcgacaacga
    51541 agaatgaatt cgtcgttcat tatcaaatca aaccattgga cgattcgacc tgtgaggttc
    51601 attacacaga aaaaatggag tcttatggca tgcttcaaaa gttgaatgat atgcttttag
    51661 gaacaatctt gatgtatttt aaacggagac gtttcaaaaa aatgctagat atgatcgaag
    51721 caacgtatta aatcaatgaa acatggtact atttagttat tgaaaaatga agtaagctag
    51781 aaggaatttt tcataggaag atagatcttt cctgcgaatc ctcactagct tttttgtagg
    51841 aaggggcagc aaaatgacaa acaaagtaag agagctaggt gtatcttcga ttgaggaaat
    51901 gtttgattta gccacttatg catttaatgg ggcagataca cctgaaaggc gagaacgttt
    51961 tcgtacactt gcggagaatt cttggaatta cggatttttt gatgaggagg atcggctaac
    52021 aagtcaagtc atggcgaccc cttttccagt gaatttttac ggacaagagt acctaatggc
    52081 cggcattggc tatgttgctt cttatccaga agcaagggga caaggcggga tcaatcagat
    52141 catggaaaag atccttgaag attgccgtga tcgtaaagta agcttatctt atctggcgcc
    52201 attctcctat cccttctatc ggcgctatgg ctatgaacaa tcatttgata aaatcagtta
    52261 tcaactaaac agtcgtgatg tgccgtttat caaaaaaaaa tctggaaaaa taaagcgtat
    52321 ggattttgaa gatgcaaaag ctgcaatcag aagaatttat gaacagatgc cggaaaacca
    52381 acgagctggt ctgaagcgcg aagactggtg gtatacgtat aaattcaagc aaaaaggcaa
    52441 ttaccagttt gctttgcatt ttgatgataa tgaggaagca acgggatata tcgtttatca
    52501 gcttgcaacg tcttccttta ttattgcgga gtggggattt ttggatcatg atgctttttg
    52561 cagccttgtt cgcttcgtaa gttctcacaa tggtgctttt gaaacatttg aatattcatg
    52621 cggctacggc ggtaatgatc aaaattatct attagaaaat ccatttgctt ctgtaatgat
    52681 cacaccttat atgatggcga ggatcgtaga tatttctttg tttttggaaa attatccatt
    52741 taaaaagaga caagctgcat ttgccctaca aatagaagaa gatcattatg cgaaatggaa
    52801 tgaaggaatc gtggaagtaa caatcgaaaa tggaaaaaat catctctcaa aagtagaaca
    52861 aacaaaactc ccaatcgttc gaggaagtat ccaacatatc acgcagctat tattagggta
    52921 tcgtaaagtg gaagagttgg tattccaaga aagaatacag gtgacaaaag aatatgaaga
    52981 aacgttaagc tcaatcttgc cacagcaaca gccaattctt aatgactatt tttgataaaa
    53041 aacgggtgat gacaatattt ttgtcaccac ccttttgttt ttcatttggc atttgaagga
    53101 aaatgttaaa gtaaatcctc ttttttcgta aatttgaatt ttttgttttc ttttgtttta
    53161 gggttttctt ttttgtggtc ttggattgct tctttcgtaa tcttttcccg atttttttta
    53221 gatgaatcta agcgttctgc gtatactttt tcttttggct tatcattcat tggaaaggac
    53281 ctccctacat tatttcattc tacctctagt ttatcatctg ttaaggacta gtggaaacgt
    53341 tttgcctaac ttataagctg atttttttag cagataagct tgcaagtaaa aaacaaacaa
    53401 gctacaattg atttattgaa attgttcctg tagctcagta ggatagagca atcgcctcct
    53461 aagcgatagg tcgctggttc aattccagtc aggaacgtat aaataacccc tgtatccttt
    53521 gcggtacagg ggtttgtttt gtatttgtgc caaaaatgtg ccaaactagt tatcattttt
    53581 atataaacta tcgaacacag tattgatttt ttctttatcc tgatcttcaa gctctcgaac
    53641 aatatgaaga taagtagaca ttgtagtttc taaactgcta tgtcctaaac gtttcgatac
    53701 gctaagtata ttt
//
LOCUS       DAZUMK010000006        49873 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000006 DAZUMK010000000
VERSION     DAZUMK010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.006"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            161..1381
                     /locus_tag="ACG808_000373"
     CDS             161..1381
                     /locus_tag="ACG808_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFS5046032.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            1378..4098
                     /locus_tag="ACG808_000374"
     CDS             1378..4098
                     /locus_tag="ACG808_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5046033.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            4082..5026
                     /locus_tag="ACG808_000375"
     CDS             4082..5026
                     /locus_tag="ACG808_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS5046034.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(5095..6105)
                     /locus_tag="ACG808_000376"
     CDS             complement(5095..6105)
                     /locus_tag="ACG808_000376"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS5046035.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(6285..6629)
                     /locus_tag="ACG808_000377"
     CDS             complement(6285..6629)
                     /locus_tag="ACG808_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFS5046036.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(6622..7056)
                     /locus_tag="ACG808_000378"
     CDS             complement(6622..7056)
                     /locus_tag="ACG808_000378"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFS5046037.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            7194..7931
                     /locus_tag="ACG808_000379"
     CDS             7194..7931
                     /locus_tag="ACG808_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5046038.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            7924..9135
                     /locus_tag="ACG808_000380"
     CDS             7924..9135
                     /locus_tag="ACG808_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5046039.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            9339..10121
                     /locus_tag="ACG808_000381"
     CDS             9339..10121
                     /locus_tag="ACG808_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFS5046040.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            10191..10853
                     /gene="trmB"
                     /locus_tag="ACG808_000382"
     CDS             10191..10853
                     /gene="trmB"
                     /locus_tag="ACG808_000382"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFS5046041.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(10951..12705)
                     /gene="ade"
                     /locus_tag="ACG808_000383"
     CDS             complement(10951..12705)
                     /gene="ade"
                     /locus_tag="ACG808_000383"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFS5046042.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            12837..13766
                     /gene="comGA"
                     /locus_tag="ACG808_000384"
     CDS             12837..13766
                     /gene="comGA"
                     /locus_tag="ACG808_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFS5046043.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            13723..14772
                     /gene="comGB"
                     /locus_tag="ACG808_000385"
     CDS             13723..14772
                     /gene="comGB"
                     /locus_tag="ACG808_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFS5046044.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            14769..15047
                     /gene="comGC"
                     /locus_tag="ACG808_000386"
     CDS             14769..15047
                     /gene="comGC"
                     /locus_tag="ACG808_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFS5046045.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            15022..15492
                     /gene="comGD"
                     /locus_tag="ACG808_000387"
     CDS             15022..15492
                     /gene="comGD"
                     /locus_tag="ACG808_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFS5046046.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            15464..15811
                     /locus_tag="ACG808_000388"
     CDS             15464..15811
                     /locus_tag="ACG808_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046047.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            15774..16199
                     /gene="comGF"
                     /locus_tag="ACG808_000389"
     CDS             15774..16199
                     /gene="comGF"
                     /locus_tag="ACG808_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFS5046048.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            16171..16530
                     /gene="comGG"
                     /locus_tag="ACG808_000390"
     CDS             16171..16530
                     /gene="comGG"
                     /locus_tag="ACG808_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFS5046049.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            16702..17709
                     /locus_tag="ACG808_000391"
     CDS             16702..17709
                     /locus_tag="ACG808_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5046050.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            17734..18918
                     /locus_tag="ACG808_000392"
     CDS             17734..18918
                     /locus_tag="ACG808_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFS5046051.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(19355..20938)
                     /gene="abc-f"
                     /locus_tag="ACG808_000393"
     CDS             complement(19355..20938)
                     /gene="abc-f"
                     /locus_tag="ACG808_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFS5046052.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(21043..21171)
                     /locus_tag="ACG808_000394"
     CDS             complement(21043..21171)
                     /locus_tag="ACG808_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046053.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(21516..21659)
                     /locus_tag="ACG808_000395"
     CDS             complement(21516..21659)
                     /locus_tag="ACG808_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFS5046054.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(21882..22361)
                     /locus_tag="ACG808_000396"
     CDS             complement(21882..22361)
                     /locus_tag="ACG808_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS5046055.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(22434..22718)
                     /locus_tag="ACG808_000397"
     CDS             complement(22434..22718)
                     /locus_tag="ACG808_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046056.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(22796..22990)
                     /gene="ssrS"
                     /locus_tag="ACG808_000398"
     ncRNA           complement(22796..22990)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACG808_000398"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(23062..24348)
                     /locus_tag="ACG808_000399"
     CDS             complement(23062..24348)
                     /locus_tag="ACG808_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFS5046057.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            24486..25157
                     /locus_tag="ACG808_000400"
     CDS             24486..25157
                     /locus_tag="ACG808_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFS5046058.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            25123..25611
                     /locus_tag="ACG808_000401"
     CDS             25123..25611
                     /locus_tag="ACG808_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFS5046059.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            25624..26571
                     /gene="prmA"
                     /locus_tag="ACG808_000402"
     CDS             25624..26571
                     /gene="prmA"
                     /locus_tag="ACG808_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFS5046060.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            26572..27336
                     /locus_tag="ACG808_000403"
     CDS             26572..27336
                     /locus_tag="ACG808_000403"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFS5046061.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            27569..29782
                     /locus_tag="ACG808_000404"
     CDS             27569..29782
                     /locus_tag="ACG808_000404"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFS5046062.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            29793..30239
                     /gene="dtd"
                     /locus_tag="ACG808_000405"
     CDS             29793..30239
                     /gene="dtd"
                     /locus_tag="ACG808_000405"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFS5046063.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            30410..31669
                     /locus_tag="ACG808_000406"
     CDS             30410..31669
                     /locus_tag="ACG808_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFS5046064.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(31758..32705)
                     /locus_tag="ACG808_000407"
     CDS             complement(31758..32705)
                     /locus_tag="ACG808_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFS5046065.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            33099..34391
                     /gene="hisS"
                     /locus_tag="ACG808_000408"
     CDS             33099..34391
                     /gene="hisS"
                     /locus_tag="ACG808_000408"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFS5046066.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            34406..36175
                     /gene="aspS"
                     /locus_tag="ACG808_000409"
     CDS             34406..36175
                     /gene="aspS"
                     /locus_tag="ACG808_000409"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFS5046067.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            36284..37159
                     /locus_tag="ACG808_000410"
     CDS             36284..37159
                     /locus_tag="ACG808_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS5046068.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            37425..39308
                     /locus_tag="ACG808_000411"
     CDS             37425..39308
                     /locus_tag="ACG808_000411"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFS5046069.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(39383..39667)
                     /locus_tag="ACG808_000412"
     CDS             complement(39383..39667)
                     /locus_tag="ACG808_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFS5046070.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(39657..40391)
                     /locus_tag="ACG808_000413"
     CDS             complement(39657..40391)
                     /locus_tag="ACG808_000413"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFS5046071.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            40520..41143
                     /locus_tag="ACG808_000414"
     CDS             40520..41143
                     /locus_tag="ACG808_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFS5046072.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            41335..42456
                     /locus_tag="ACG808_000415"
     CDS             41335..42456
                     /locus_tag="ACG808_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFS5046073.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            42463..45591
                     /locus_tag="ACG808_000416"
     CDS             42463..45591
                     /locus_tag="ACG808_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5046074.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            45610..48804
                     /locus_tag="ACG808_000417"
     CDS             45610..48804
                     /locus_tag="ACG808_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFS5046075.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(48950..49657)
                     /locus_tag="ACG808_000418"
     CDS             complement(48950..49657)
                     /locus_tag="ACG808_000418"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5046076.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16256 a         8790 c        10415 g        14412 t
ORIGIN      
        1 gtgacaaaaa tcatgtcaca atcttttttt cgaataaact tattttttga agtcggccat
       61 ttatcgtaat ttctttgcat aatacgaaca aattttcggt tattgatgaa aaaaaacatt
      121 gaaaatggta tgataaaaga aaaaaaggag gaaattttta atggtgaaat ttatccatgc
      181 ggcagattta catatggatc ggtcatttga aggactgaca actcttgata aaactgtaca
      241 ggaaaaacta ttgaagacga atctgaccgt tttatcaaat atcattgaac aggcaataat
      301 aaataacgtt gattttgttt tacttgcagg agataatttt catcaaaatc gtccttcatt
      361 aaaaatacag aaacattttt cagaacagat gaaacggtta gaaaagaacc atatacctgt
      421 atttataata ttcggaaatc atgatttcta tcaaaaagag cggtattggt tctcctttcc
      481 taaaaatgtc catttattta catccgaaac ggtagagaca aaaaaaataa cgataaaaag
      541 tggagaaact gtctctctca gtggatttag ttatcgtcaa ccgtggattc aaaaagacaa
      601 agtgatggaa tttccttcta gagaactcac agattaccat attgggttgt accacgggga
      661 acctggcgtc tctcaaaaag gaaactatgc tcctttccaa ccctcaaaga tgcaagaaaa
      721 gggatacgat tattgggctt tagggcatat ccatgtgcca acggtcttaa atgaaaagca
      781 aacaattgtt tatccaggtg cgcctcaagg tcatactaaa aaggaacaag cttctacatc
      841 tattctacta gttgaattaa gtaaaggaag ttgccaaata catccgatta aagtagcaga
      901 ggtctattgg aagacaaagg aaatatcatt gaggaaagca agaacgacaa aagaggtcat
      961 tgttcacatt cgtcagcagc tttcaaaagt agaggatgga tttagtttaa ttgagataaa
     1021 attaaaagat tatgaccatc tcaatacaga tgttttggaa cggatacaat caggagagct
     1081 attagactat ttgttagagg aattctcaga taggataaat gatttcttta tttggcgtat
     1141 ttctctcata gagaatactt tttatacgaa gaccccactt gctgcttctg ctaaattgat
     1201 ggaacagtta tttcaatact atcagacacc tcaagatttc caacaaattt taaatgaagt
     1261 atatagtcat caagaagcgg ctagaatatt gagcgaactg ccggaatatc aagaagaaac
     1321 acttgaaaaa gccaagcagc tgcttaatca agatttttta tttgaggagg atcaggaatg
     1381 aagattttaa ggatcgagat tgctgctttt ggaaaatggc gtcaaaaaag ttttgacttt
     1441 tattcaggca atcagctgat ctatggggga aacgaagcag gaaaatcaac gatttatcaa
     1501 tttattcaag caatattatt tggtttccct tcaaaaggcc gtaaaaaaaa ggattataca
     1561 ccaaaagacg gctcagccta tggcggcaaa atatggctta aacatcctgt atatggtgaa
     1621 tttgctgtcg agcgctacaa gcagcaaaat cgcggaaaat cgaaggtgtg gctgggggat
     1681 caagtgggaa gtgatgagtt actagaaaaa ctgatcgcac ctttgacgaa agagttattc
     1741 cagcaggtat ttactttcca gcaagagcag ttgatggaac tagatcattt gagagaaaaa
     1801 gagctacatg atgcgctgat ttctttaggc atcacgggca gttctcaagt attccaaaag
     1861 cggcaagaat attatcagaa agcacaacaa ttattcaaga aaaaaggcca aaaattacta
     1921 atcaatcaaa aattaaatga atggaaagag ctgcaagaaa aaattcatca aaaacaagaa
     1981 caggaagctt attttggcaa gttgatccaa caagaacagg aatacgagtc gcaacagcag
     2041 cagttattgg cacaattaaa agaggcgaac aaacaatttt cattagccag acagcaagaa
     2101 cttaattttt ctttatatga agaatggcaa tctctcaaaa aaaacagcac tacaggtctt
     2161 cctgatgagc aacttctttt agatctggag tctttctcca aacggtatca gcaaacaaat
     2221 gagcgtttgg aagaattaga agcaacactg gaaaagcata gcgggatgga tcagcagtca
     2281 gccagatatt atttttattt agagcaggag caggaactaa aagatctcca gcaacttcaa
     2341 tttacagcgg aacaattaaa cgaagaatgg caaagattac aaattgagac tagagaaaca
     2401 aattcagaat tgaagatttt ggaacaaagg tggcattggc agaaaaatcc gttaccggaa
     2461 gaatttttat atgaagaaga atggaagcat cttctggctg aagaaagaag gattgctgat
     2521 ctagaaagcc agatttctgt gcgtctccaa atagctaaag aacagcaaga gatgattgaa
     2581 gcagaagtga atgacttgga agagacctat ccagcattat tacaaaatca gtcagagata
     2641 gcaccagtct cttcatctaa accgtatatg ctgatggctg gcggagcaat aatcattgca
     2701 ggtatatttg taccaatgcc cttgaaagtg atccttttac taattggtgc aggcatgatt
     2761 ggctatttct tctacaaaag gcaaccaaaa attaaaacta aaaatgattt ctctgagata
     2821 aaaggaatgt ggcaagaaaa actaggacaa cttgatctag ccttagctca attagctgaa
     2881 gcagaagaaa aacaaaagag aactggagaa gaaaaacaga ttcttcagca agaagtatat
     2941 cgttttgcgg atcatcggca cttaggaaag atggatacgc tcaaactcat gaaagaatat
     3001 ggacaggaag tcatagatta tcagaaatta tatggcgtat ttgagcggaa acataaaaga
     3061 gaacaggaaa tacaagaaaa actacgacaa atcgaccaaa aatttgagtt tgttaaagaa
     3121 tggttaccga tacaagataa gacaatttca gaaaaaatgg tattgctttc ccgttttata
     3181 caagagatgg aacagatgaa atttgccaga agctatcagc aaaatacttt attgaagcaa
     3241 gaaatcaatc aattgaaaaa gaaacaaaaa gtatggatac aagaatacag cgaacaattg
     3301 actcaagcag gcttatctta tccttcggaa gtacctgtct ttttacaaaa agccaaacaa
     3361 gccgaacagc agatgaaaag atacaatgaa ttatcggaaa tgcttactcc actatttcct
     3421 gaagagctga caaaagagaa attatctgta agactgcgcc aactgaaaga aaagcaatcc
     3481 caactccagc aagaagaaaa agaagcaagt gaaaatcggc aacgactcca acttcaagta
     3541 gaagagttgc aaagagacgg cacattggac gaactttatc aacaagctag ccggcaaaaa
     3601 gcaaaattac aggaattact ggaggaatgg ggagcacttg aaatcgccgg gacattctta
     3661 ggtgatcttt caacagagat gagtgaaaga caattacccc agttgttaag agcagcaggt
     3721 gattatttag gtattttaac aaaaggaaat tatcagaaga tattactgaa aaatgataca
     3781 ctgactttaa cagacggata tacagaattt cccatctatg agttatctac aggtacgaaa
     3841 gatcagctga ttatggcgat gagatttgct tacctctcat tagaagctaa ccgttcgata
     3901 tgcccgatca tcattgatga tggctggctt cattatgatc atcagcgaaa gtatcaatta
     3961 gctgaacttt tagctaaatt tggagaaaag tatcaaataa tttgtttttc ttctgataaa
     4021 gaaatggtaa gctattacca tgagatgaac caaatcgtaa aacaattaga aggagcaaat
     4081 aatgaaaaaa attcgtgatt tagtaatcga tgagcagttt gaacaatttg tcctgatcaa
     4141 aagtgcagat gtaagaatcg ctaaaaacgg aaaaaaattt attgctttta ctttccaaga
     4201 tacttcaggt actatcgatg gtaaatactg ggatgcttcg gaggaggaaa tcaataaatt
     4261 tacagccggc acagtcgttt ttctaaacgg taaaagagag gtttatcaag gaaaccctca
     4321 agtaaagatt cttcatatgc gcttaacaag ggtagatgag ccaggagagc ctagtttata
     4381 catggaaaga gcccctttga agaaagaaga aatggaagaa gaaatcaatc aattgttgtt
     4441 tgagatcacc aatgcgcatt ggaatcgtat tgttcgctat ctacttaata aatatcaaaa
     4501 acaatttttt gagtttccgg cagcaaaaag aaaccatcat gcatttgcca gtggattagc
     4561 ttatcatact ttgacaatgc ttcacttagg gaaatcaatc gccgatgaat atcctgagct
     4621 gaatcgagca ttgttgtatg ctggtatcat tttgcatgat ttaggaaagg tgattgaact
     4681 ttccggtcct atgtcgacag aatatacgct tgcaggaaat ttgatcggtc atctggtatt
     4741 gattgacgaa gaaataacta aagcctgtct tgcgttgaaa atcagcgaag ctgacgaaga
     4801 tgttctcgta ttacgtcata tggttttagc acaccatggc ttattagaat atggctcacc
     4861 tgtacgtcca agaataatgg aagcagaaat attacatcaa atcgacaata tcgatgcttc
     4921 gatgcagatg atgttgactt cgattcgtca aacagaacca ggaaaataca ccgatcggat
     4981 tttcggtatg gacaaccgaa gtttctatgt accaaaagat ttatagcaaa aatttatttt
     5041 tttcattcaa aacaaaaaaa gcgagacata agtcttcgct ttttttgttt tcttttattt
     5101 tgatgaatca gttgtttctt cagttgaaga ttcttttgtt gaatcagttg attttgtgtc
     5161 agaactcttt gttgaagctg tagtttcact tgagtctttc gtacttgatg aagttgttgt
     5221 gaatgcactc aacacatttt caaaggcatc atctttgatt tttacatttg catcttttaa
     5281 ttcttcaccg attactttag ttgtgaatgc attgtcactt agtttagttt cagtagcgat
     5341 atcttttagt tgatctttgt atttatccat gtcattgcct ttattttggt ttttcaccat
     5401 tttgacaacg tagtattctg tcgcataaga agtaggattt gttgtagtaa taacatcgga
     5461 gatctcaccg tctttcaatt tgaaagctgc ttcttttact tctgcaggaa tagttgttgt
     5521 cgttgagtcg aatttaactt taccgccatc ttcttttgtc tctgtatcag ttgatttgtc
     5581 tttggctaat ttagagaaat catctccatc atccgctgat tttttcacat cttttgcttc
     5641 atcttcactt gataatttga tgatttgtgc ttctacttct ggatggaaag atttccaagc
     5701 agttttcaaa tcgtcatctg taatatctac gtgagctttc aagccagctt caaaagctaa
     5761 attgttacga atatattctt tataagtatc tttcgtatat ccagcagctt ctaattgtga
     5821 ttcaaaagta tcacctaatg atttcgcttg tttgtcgtat tctgcatcca cttgcttatc
     5881 ggttacttta tcgccatatt tgttattgaa tactttataa atgatcatcc gttgtactaa
     5941 ggattggttc gatgattcaa gttttgcttc atcatagaaa tcagatacag tgatcgttcc
     6001 gcctttcatc gttgcgatat ctttattagt atcgcctgaa caagctgcta gagttaaaac
     6061 agccaacgca ctagtagctg ccaaaataat tgattttttt ttcattaaaa tttgcactcc
     6121 tgtaattagt tattattttt atatattaac tataccatat cccatttcct ttaaaaacgt
     6181 tgtcgtggta gtttcaaaca aaattcaaaa tttcatcaca ttcctaggaa atacgcaaac
     6241 ttttctcact tatatagtta aatctgttga gaaaataatc attattatat ttgttcagga
     6301 tactctggca gatcagcttt tagtttttct acttgttcat tgatttgtgt gatacgtggt
     6361 tcggcttgga atttaaaatc ttcgatatct tttttgattg cttcttggaa agcaggaatc
     6421 acagattcag ctgtttcttt tgtttcgatc aatgcgcttt taaaatgatt caggctgtta
     6481 tttaaatcat ttgtcagagt tgttgcttct tccaattctt cgatcagttt ctttcttgtt
     6541 tcattcccgc tcctgggcgc caataacaga cctccgacag tgccagcagc agctccaaat
     6601 aacagtcctt tgaaaaagtt tttaatcatc gcaggacacc tgctttacga ttgtttcttg
     6661 aatagctttt agttcttccg atccataact atcttgatga ttgacaaagt gaatagaaaa
     6721 atcatcctct ttgctgtagc gagggactaa atggatatgt gaatgaaaaa cagattgata
     6781 ggctagttca cggttgttat tcaagatatt aagacctttc atttctggaa aagctttttc
     6841 caatgcttga gcaatcttag gcacacgcga aaaaacctct cctgcaagtt cgggatcata
     6901 ttcaaaaata tctgctacat gacgtttagg cacaactaat gtgtgtcctt tcgttgcttg
     6961 agagatgtct aaaaaagcat agaccttgtc gtcttcatat attttatagc ttgggatttc
     7021 ttgattgatg attttgcaaa aaatacagtt ttccatataa aacatccttt ctataaagaa
     7081 taaatacttc agcttaactt taccataagt taaagctatt gtaaggaaaa aaacaaaata
     7141 ccgtcatttt cttcgtggta taataaataa aaataaatgg cggaggaaaa agaatgagtt
     7201 taattatcga tcatgtcaca ggggggtacg gacatctccc tgtgttaaaa gatatcagtt
     7261 tcgatgtaca gcctggagag atagtagggt tgattggact gaacggagca gggaaaagta
     7321 cgaccatcaa gcacattatc ggtctgctgg aagcttcaaa aggaacgata gaaatcaatc
     7381 atctatcctt aaagaaaaat acggaagaat atcgcaaaca aattgggtat attccagaga
     7441 ctccttcttt atatgaagaa ttgacattga gagaacatat cgaagtcact gcgatggcct
     7501 acgatatacc tatggatata gcaatgaaaa gagcagaaaa gttgttggag acttttcggt
     7561 tatctaatcg tcttgactgg ttcccagcta atttttcaaa gggaatgaaa cagaaagtga
     7621 tgatcttatg cgcctttttg atcgagcctt ctttatatat catcgacgaa ccatttttag
     7681 gcttggatcc actagctatc aatgcattgc ttgaattgat ggtagaaatg aaaaaagaag
     7741 gagccggtat tttgatgtct acgcatattt tagctactgc agaaaaatat tgtgatcgtt
     7801 ttgtggtctt acataacgga gagatacgag cagacggttc tcttgagatg ttgagaaaag
     7861 aatttcagct gccggattct tctttggacg aaatctatat tgctttaaca aaggagcagg
     7921 cacatgagta gtttttttca aaaacgttta gcaagacatc aaaagaaaat gatgaaatat
     7981 atgcgttatg tattgaacga tcattttgtt ttagtttgtc ttttcttgat tggcggtgtg
     8041 ggattatatt attctaattg gttgaaaacc ctgcagccac cgtttgcaat tggagggctc
     8101 ataatctctg tattttggat gctctgttta ttcgtgggta agatggctac ttttgcagag
     8161 gcagcagata ttgttttttt acttcctaaa gaaaaagaaa tgcaaaccta tttggaacga
     8221 gcttttcgct attcttgcat ttttccgttg atctttttga tattggcatg cggattggcc
     8281 atgccattag tggtggttag tacggagcaa tccttttcga tgtttttcgt ctatctgatc
     8341 atattatgga gtttgaaatt cagtcatctg caaatcaaga gagcagaatt gtttcgacta
     8401 gatcaacaag ttattcgtta ttggaaatta ggctggtttt tttcttctct gatctttttg
     8461 tttctatcat tttattggct aatatgggca ggaatagcag gtgcattagt acaggcaagt
     8521 ctatattact ggtttttgtg gaagcaacaa gaaaaaggat tggattggga gaaaatggtg
     8581 catgtagaag aacataggtt gcatcagatc tatcaattta tcaatttatt cacagatgtt
     8641 cctgaaatta cggcaaagat caaaagaaga cgctatttgg atcctatatt gactcatatt
     8701 aaaagagaat caaaaaacac ttatctttac ttgtttgcaa gaagattggc aagaggttct
     8761 gattttagtg gtctttattt ccgattagta attataggtg ggttattgat tgccggagta
     8821 gtcgactttt attttagtct gattatcggt ttcttatttt tatatttgat cggtttccaa
     8881 ctgttacctt tagcgaacca gttccgctat atggttctcg ttcagcttta tccaatccat
     8941 aagaatcaga agaagcaggc gattcagtat ctactcacat gggtattaat tatcgcgtcg
     9001 gttatttttg gtttgattgc tgcagtcgta ttagatgggc agcagcgatg ggttccgcta
     9061 gcggcttatt tgattgttac tatgctcttt gttaaactat atgtaccaac tcgtttgaag
     9121 aagatgtcag actgaaaaca aagctgtgta gggtaaaaat gagaatgaaa aatagagcgt
     9181 tgacagtaag aatgctgttc gttctatttt ttttgagaaa agctggtttt ttgaatggca
     9241 attaaatgaa aaagaacgta tatgtgcttg acgtgtagtg ttcaggcgac tagaataata
     9301 agtactgcaa aacaaagagt aagaggacgg gagaatgcat gatggagttt cagttagacc
     9361 aagagtggcg gttacgtccc atcaaaggcg acacaggaaa aacatacata ggcatgcgtg
     9421 atgaggacaa agtctttatt aaacgaaaca cgactccgat gttggctgct ctttcaaggg
     9481 aaggaattac accaaaactt gtttggacaa aacgaacggg gaatggcgat actttgacag
     9541 cacaagagtg gctggaaggt cgcttgctga ctcctaatga gattggtcaa agaaacgacg
     9601 tgattgatgt tttgtaccat ttacatcatt caaattctct taaaaccatg ctgacccgta
     9661 tcggtggtcg tgtcatgacg ccggagaaga tgctgcaaaa atatgaaaaa gaattaccgg
     9721 aagaactcca gaaaaatcaa tttctgcaaa ttgtccatcg ttttcttcgt gagcagatcc
     9781 cgtcaataga taaggaaaac ttatcagtag tacacggaga tgtaaaccat cgtaattggt
     9841 tagtatgtaa aaattattta tatctagtcg attgggattc aatcatgttt gcagaccctg
     9901 cggtggatat tgcaacgatt ttaggaaatt atgtgccact gtctagctgg aatcagtggc
     9961 tgatcagtta tggcatacgc ccgactgacg aaatgcttga aaaatttcat tggtatgcgg
    10021 ccatgaattt gctccaagaa atcaccagat attgcaaacg tgaagatcat cgacggatga
    10081 acgaagaaat tttacaatta aaacgaattt tcagtggctg aggtttggcc caaactctgc
    10141 ggagatttgg ccagaccttt ttaattagag gaaaggaatg gagataaata atgcgtgtcc
    10201 gaaatcgtcc gggagcagcg gaaatgttag cggctcaccc gaattttgta attagtgatc
    10261 caacattgtg gaagggaaaa tggaatgaat tgtttgaaaa cgatcatcca atccacattg
    10321 agattggtat ggggaaaggc cagttcatca ctggaatggc aaaagcccat cctgaaatca
    10381 attatattgg agtagaaatg caggtcagtg ttgtttcgat tgcactagac aagttgatcg
    10441 aacaaccgct tcctaacctg aaacttctac atgtggatgg atcagcgttg acagagtatt
    10501 tcgctgacag cgaagtagat caaatctatc tgaatttttc cgatccatgg ccaaaaaaac
    10561 gtcatgaaaa acggcgattg acatataaga cttttcttgc tgtagatgaa cagattcttc
    10621 gtccaaatgg agaaatccat ttcaaaacag ataatcaagg attgtttgag tattctctgg
    10681 caagtttttc tcaatatggc atgatcctca aacaggtatg gttagattta catcaaagtc
    10741 aatttgaagg caatattatg acggagtatg aagaaaaatt ttcttcaaaa ggtcaacgta
    10801 tttatcgagt agaagctcaa tttcaagata agaccaaaaa gaaaagtctg tgaaactaac
    10861 tttctgcttt cataaaaaca ttaatatcca taaataaatc ccagaaaatg aacattacag
    10921 atgtaatgta ttttctggga tttatttgat ttaaaatgta tttatttcat cggtttttcc
    10981 acaaggtgga atcaattgga agcgagaaaa tgaaaataat cctttgtcgg tgatcttcaa
    11041 ttcgggtatg acagataatg ttaagaaaga aagtgtcaaa aaaggatcaa aggttttagc
    11101 tgctcctagt tgatgtgctt tagttattaa ttcattcaac tgttcataga cgatcgaaaa
    11161 aggttcctgt gtcatgattc cgcctatagg aagagaaaca caagctattt cctgctgttt
    11221 gttgacaacg gtcatcccgc cacctttttg aatcaaaaga ttagcagcat acagcatatc
    11281 ctcgtcgttt gtcccaacga caactagatt atgcgagtca tgggcaatcg ttgtcgctat
    11341 cgctccttgt ttcagctgaa atcctttcac tatgcctaat ccaatgtttc ccgtttgatg
    11401 gtgacgctcg atcaccgcaa tctttaattg gtctttttcg attgaaggaa tgaatcgttc
    11461 gccttgtcgg tccacttttt ctaccaaatc tttagtcact agactattcg ggataatttc
    11521 gatcacatgg gcttgtgaag aagaaagaag cagctctaac gaatgtggat ttaatggttg
    11581 agcgtttagt tttggcagac aacacgtata tgaatttgaa tgatcagcaa ataaatggtc
    11641 ttggagttgc ccagattgaa caactaatgt tcccttatag aaaacttcat gaattggcag
    11701 cttggtaaga tcgtctgtta agaagaaatc agcttgatac ccaggggcaa tcgcacctag
    11761 tgttttcaaa tcaaaacatt ctgctgcatt caatgttgcc atctgaatgg cttgtaatgg
    11821 atcgattcca agtgcaatag cttgacggac gatatgatcg attgatcctt cttttactaa
    11881 atcattgatc agtttatcat cggtgacaaa gaagcatctt cttgaatttt caggtgtgat
    11941 cgccggtagt agcgatgcca gatcttttgc aacggtccct tctcgaacca ttaaatacat
    12001 accaagatcc aaacgttctt tagcttcttt tgcagttgta gcttcatgat ccgtccgaat
    12061 acctgctgca ggataaacat ttaactcttc catgccgata ccagctgcat gcccgtcaat
    12121 ttttttctta tgctggtgca tcaattggat cttttctagg atgtctgttt cattatttgc
    12181 tactgcttga tagttcatga cttcagctaa gcctaaaact tcgggttttt gtaaaaaagg
    12241 ggctaatttt tttgcattta aatttgcacc attggtctca aaaggggtta caggtacaca
    12301 agaaggtagc ataaaaaaga tattcatgag tgtttgtctt gcatcttcta tcataaactc
    12361 aagacctgta ctcccagcaa tgtttcctat ttcatgggga tctgtcacta cagtagtaac
    12421 tccatgaagc aaggatactt ttgaaaattc gcttggtgat aaaagtgagc tttcgatatg
    12481 cacatggcca tcgatgaatc ctggcactag atattgccca ttagcattat gtacgctttt
    12541 tccttcgtat tcaccgattc ctacgatcat tccttcgcag attgctacat ctttttccat
    12601 gatttctttc gtaaagacgt tgacgatctt agcatgcttg atcactaggt cagcaggctt
    12661 ttttttactt gctacttcga tatatttcct taatgttgtt tccaatggag tgcctcccaa
    12721 gataaatttt tataagtata acataaattc ggtttttgcc aatatttttt tgtaaattca
    12781 ttttatttga aacaaaaaga tcaaaaagac gtatgttttt tgtggagggg agaataatgg
    12841 aaataaaacg attatcggat caaatgataa gtgaaagttt tgccaaacat gcccaagacg
    12901 tatatatatt gccagcttca gaagtatttt acatctattt tcgctatgga aaaaaacgaa
    12961 cattctggaa gaaagtctct tcggcagatg gacagcaact gatctcaaga tttaagtatt
    13021 tgggacagat ggatatcgga gaaaaaagaa aagctcagtt aggtgcattt acttattctt
    13081 tagaaaaaca ggaagttcgt ctgcggttgt cttctgtagg agattacctt caacgagaga
    13141 gtttagtgat tcggctatta tacggtactt ttgaaacacc ccatcgttgc tttagttctc
    13201 gagattttgc aactatttta cagaaaaccg tcagacgtgg attatacctt ttcagcggac
    13261 cagtcggatc aggcaagact tctctaatgt atcggttggc tttggaagaa aaattgcaag
    13321 tgattactat cgaagatcct gtagagatag aagaaccgtt ttttctgcag ctacagatca
    13381 atccgaaaat agcacagact tatgatacac ttttgagact cgcattacgg catcgtccag
    13441 acttgctaat cattggcgag ataagagatg aatcaactgc acatgctgcg gtaagagcag
    13501 cactaacagg gcaccgggtt tttgcaaccg tacatgcacg caatcttgaa ggtacattgg
    13561 tcagaatcca tgaactgaca aagaaaaaag aagagctatc tgaatgttta gctggtgtca
    13621 tttatcaaga acttttggaa gatgccaatc accatccttc tgttttatgg agttatcaat
    13681 ttatggagga agaatcagta aaaaaatcat gggaggaaag ttatgaagaa gctcaggaaa
    13741 atatttggca gtcagaacta ttctaaagga ttaacaagaa gccagcaaaa ccagtttgca
    13801 caaaccatgg gagaactgct tttatctggt tttagtctgc aagaatccct ttatttatta
    13861 ctcacgatcc gttcgtttcc acctgttatt ttggcaaata tccaacatca gctaaaggaa
    13921 gggcaagcgt ttagtgaggt actttcgcaa ataggatatg gagcagagca actggtacag
    13981 atcgagttag ctgaacgtca tgggaatctg ccgacaactt tgttggaaat tgccaaacag
    14041 tttcggcttg ttgaaggatt tcgcaatgat ttgaaaaagc tgcttgcata tccgatgcta
    14101 ttgctcacct ttttgctagg tatcttgttt agtatgcggt tgttcgtttt gcctcagctt
    14161 attggttcag gaatggtcat gcaagagcat tggggtattc agctgataca agtctttcat
    14221 tggtatgttc tactattcat cctagcggtt actctattgg ctttactagt aaggtatcgt
    14281 ctgatgaagt tgtctcctgt tgatcagagt gaatggataa gcagacaatt attgattgga
    14341 ccgatatatt ccttgtatca atcggcttat ttgtcactag agattggaaa gctttttcat
    14401 gaaggtttcg aattaaggca aatcatagtc tgcttaaagg aaacgaaaca agaaagtctg
    14461 attcagcatc ttgcctctaa gttgattcaa ggattggagg caggtatgtc attggctgag
    14521 cagttccaga attatcgttt tctatcagaa gaattcagta aaattgtttt acagggagag
    14581 gcaaagggaa gtttaggtaa agaactgatt ttttacagcg aatggacaag aaaacagctt
    14641 ttcaaacgtc ttcatcactg gatgcatttt attcagccaa tcattttttt gggcgtagcg
    14701 gctttgattt tattagttta tgccgctgtt ttattacctg tttacggaag catagaggag
    14761 gttttgccat gaaaaagaga aaaatatctt attatagcgg ttttacattg atcgaaatgc
    14821 taatcgtctt actgatcatc agtgtattag tgttgttgtt cgtccctaat ttgtcgcgtt
    14881 ataggaatca tgtagaccaa gaaagtcgtg aagcgatcat ccagttggta gacacacaaa
    14941 aagagctcta tgcgcttcaa aataatggaa gagttcctac tgtggaggaa ttgttgaatg
    15001 aaggctatat caaacgagaa catgcagaaa tctatcagcg gccttgaagg taaggtttta
    15061 gcatcaggat atacgttgtt tgaatcactc ttactgattt tattacttac aacgatcctt
    15121 tcttttccag ttctcgcatt ttctgcatgg aagaatgaat tggcagtcta tcaatttttc
    15181 ggccaatttg aaaaacgaat ctattctacg caaaaaatcg ctattgtgaa tcagatccaa
    15241 acaggctttt attggaatag tgaagagaat caaatcattt ttcatgttcc gaatccagat
    15301 aaaataaatt gggaagtatt agatattcct gatgagatta ccttgaaacg ccatgcttct
    15361 attacttttg ctgctagaac cgggaatgaa agttcactaa aagcttatca attttattgg
    15421 gaagaaaaag agcggacgat tacgtatcaa ttccagatag ggagtggtcg ctatacaaaa
    15481 agaattgaat aaaacaggat ttgttttgtt ggagtccctt acagcgctta tgatcgcttc
    15541 tgtctgtgtc tacttagtga gctttggcca gctgtttttg cttcaggcag aaaagaaaag
    15601 tggggaggaa ctgctattta ttcgaatgat ttatgaagat atcaagacgc agcggatgta
    15661 tcacaaaaga ccagagcgaa gggaagaaat gatcccatac gatatccaaa ttgtcgataa
    15721 taatggagta atcgaaaaag tgtttgtcaa tatggagaaa gaagcgatca gtattgaaaa
    15781 aacaagtaat tccagcattt acgctgattg aatgtctaat tggactgatg atattgagca
    15841 gtctattgct cacattcaat cttttgattc agcaaaccat ccaagtaagt gaatttttgc
    15901 agcgtaaaga tcaaaaagag tggctaatat ttttagcaca attagaagaa gaactcaaga
    15961 gcagtcacaa tgtctctgtg aaaaatcagc aactgcacta cacgatcgag gataaacagt
    16021 atatcatcga aagatatcag gcaatgatac gtaaaagaag aacttccggc ggacatcaac
    16081 ctatgttaac aagtatcact gagcttcaat tattggaaaa agaacacaca atttcttttt
    16141 atgtacactt tgaaaatgga gaagatggat atgccatatg gacaaaaaac gatcagtaga
    16201 aaatgttata agggctctat tttattttct gtcttgcttt tatttctttt atgtacgttt
    16261 ttattgatgg cagcattaga aagctaccgt ttgtcaatgg atcttaatat acgaatcaaa
    16321 aattattata tagcaaaaat aatgaagcag atggctttat cggaacagtc tacgcttgcc
    16381 gaaagttcgg aaggagtttt ttactacaca actggatctg taacatatca gtgggtacaa
    16441 cagtccctat tcttagaggt ggaagtctca ccgtttatct ttcggttcac agaagaagta
    16501 aaaaatgata cagatactag tacagaatga tggaatgttc gtgaaaacgc cctcttttct
    16561 gttataacac tgtttttaat aaaaaaatgt ttgccgtttc acaaataaaa aacagtgaaa
    16621 acgattgaaa ttttgtcaat tgttcgatag aattaaacag gtgaaaaaat tgttataata
    16681 tatctagaaa tgaggtgcac catgttacca gaaaaaatgg aacaagggtt taatcaaaac
    16741 ctggaagcta ttcagctttt acagaatgca ttgggaactt cctttttaga agcctatgtc
    16801 gaaaatgcag aaaatttaat agatgattat caagtacgtg tggtggatgg agtgccgaca
    16861 aaagagacaa cgcaacggat cacagctctt tatgaagagt tgaagaaact ttctttcgag
    16921 ccagaagaat ggcgcagact gtctcaatta cttttattga agggcagcca gacagaacat
    16981 ttgcaggcga accatcaact cactcctgat agtatcggtt ttttatttgt ttttcttatt
    17041 gaacaactat acacaaataa aaaagcaccg gttaagattc tggatattgc ggcaggaatg
    17101 ggcaacttgt tattaacagt tcttttaaac ctatcgaatg cggggtacca aacagaagga
    17161 attggtgtgg atattgatga tacattgttg gctgtcgcag cttctacaag tgatttgaca
    17221 caagcaaatg ttcaatactt tcatcaagac gggttgcagg aattattaat agatcctgtc
    17281 gattttgcaa tcagcgattt gccaatcggt tattatccga atgacgaaaa agcaaaagag
    17341 tttttaacaa gtaccgaaga aggccatagt tatgcacatc atttattgct agagcaatca
    17401 atgaaatatg tgaagccgga tggttttggt ttgtttttga tgccgagtgg atttttagaa
    17461 acggatcaaa gtgaagaaat caaaaaatgg tttaaagaag aaggttatct tcaaggaatg
    17521 attcaattgc cagatgaatt gttccgtaat aaacaatcgc aaaaaagtat tttgatctta
    17581 cagaaaaaag gaccacaagc acaacaagta aaagaagtct tgcttgtgaa acttgcttca
    17641 ttaaaagaac cagagaaggt gacggaattt ttcaacgagt ttaaaaactg gaaatcatct
    17701 gctctgtaat cattcaacga gaaggagagt catatgtcta aaacaattgc aatcaatgct
    17761 gggagttcaa gtttgaaatg gcaattatat caaatgccag aagaaaccgt agttgcgaaa
    17821 gggatcgtcg aacgtatcgg tttacaagat tctattttta ccatcaagta cggcgataat
    17881 caaaaattcg aacaaatcat ggatatcgaa aaccacgaag tagcagtaaa aatgctgtta
    17941 gatcagttga ttgaactgca aattttaagc tcttatgatg aaatcaccgg agtagggcac
    18001 cgtgttgttc acggaggaga aacttatggg gattcagtag tgatcgatga gaaagtaatg
    18061 gaccgcattg ctgaattagc agaatttgct cctttgcata atcctgcaaa cttaatgggg
    18121 atcaaagcgt ttaaaaagat tttacctgac attttgagtg tggctgtatt cgatacatct
    18181 ttccactcaa caatgccaaa gcaaaattat ttatatagct taccggtgtc ttattatgaa
    18241 gatttcaaag taagaaaata tggcttccat ggcacaagtc atcgttatgt gtcggaacgt
    18301 gcggcaaaat tattgaacaa accgatcgaa gaactaaaaa tcatcacttg ccacttagga
    18361 aatggtgtat cgatcacagc agtcgatgga ggtaaatcca tggatacttc tatgggattc
    18421 acaccattag ctggggtgac aatgggtaca cgttcaggtg atatcgatcc agctgttctc
    18481 ccatatttaa tggaaaaatt agagattgat attgatgaaa tgatcaatgt tttgaacaag
    18541 aaatctggtc tgctaggcct aacaggattg tctagtgata tgcgtgatct tgaaaagaac
    18601 tatgaaaaag aacatattcg tttagcttat gacattttcg tggaccgcat ccgaaaatac
    18661 atcggcggtt atgtgacaac gatgaatggt gttgatgcaa ttgtctttac tgctggtatc
    18721 ggcgagaatg acggacatgt acgtagtgaa atcatcaaag gtatgtcttg gtttggttgt
    18781 gaaatcgatc cagcgttgaa tgaattgcgt ggtgaagaag tagatatctc tacaaaagaa
    18841 tcaaaagtaa gagtattagt gatcccaaca gatgaggaac taatgattgc ccgtgatgtg
    18901 gagcgtctgc gcggctaagc caaagcattc gttaggataa aagatgaaac gcatgcaaga
    18961 gggaattctt gcatgtgttt tttttgcgga gaagaccgca aaatggtaat ttaacttcaa
    19021 tttcccttct tttttacagg atcttcttag ggaattttgc ttggaaaagt ggtcaaaagg
    19081 gaaaagactg gcattttatt tattgatttt caggaaaatc ccaatatttt attttcattc
    19141 atctttataa gggacattac atatgtaaaa agaaaaaaca tttttagatc agcctcgtgg
    19201 gctgtcgact ttattcttcg ctttatgttt agctagaaaa ccgatccgaa acattcattg
    19261 atcgaataat aaaaacttga ttttccacct gtactttgcc tgaatcgtga aagcacaggt
    19321 atgaaaatca agttttttgt tgcgaaaaaa cgtcctagtc aatcagttgg ccgagcggct
    19381 ggcggctgat ctgctcctga tcaataacga gtgcttcttg gaagtgttgc tgcaaaaagt
    19441 agcggtcatg gctgactgct aggagcgttc cttgatagtc ttgcagcatt tcttcaattt
    19501 cttctcgagc atagctgtcg aggtgattgg ttggttcatc caaaatcagg acgttgattt
    19561 tttctaaaaa gagtttcagc aacgccaaac ggattttttc accaccggat aaatctttga
    19621 tccgtttacc cacatcgctt tgataaaagc cacttttggc taattgccgt cgagcgactt
    19681 cttcgtttcc agtcaatgtg atggcgtaag ccagcaaacg gctatcggga tcggcaaact
    19741 gcagctgctg cggcaaataa ccgatttgca agctactacc taatttgatg gtgccaccgt
    19801 aaggcttttc ctcaccgagt aataatttga gtaaggttga ttttcctgca ccattgtcgc
    19861 cgatgatggc taaatgatct ccacgataga ctgcaaaact gctgtctttg aatagtactt
    19921 gatcagcgta ctgctggtgc aagttttgaa tgatgaaaac ttctttgcca gattttccac
    19981 cattagacaa gctttgcagc cgatttttcg ggggaggtgg cggtttgacg agtgtcagtt
    20041 tcgccaggcg gcgttccagt tctttggctt ttttgaagaa ggactcattg tctccttcat
    20101 gtgcccactg acgaaaacga cgaatcatga ctttcaaccg ctggacttct ttttgctgca
    20161 gttcgtaatt ttttgtcagc tcagccaagc gagcttcttt caagactcga aaacggctgt
    20221 agtttccggg atactcaatc aatgcacctt cttccagttc ccatgttttt gtggtgactt
    20281 gatcgagaaa ttgccgatca tgagaaacga ccagaaccga tttttttgta ttctgtaaat
    20341 agttttccaa ccagcgaata ctgttaagat ctaaatgatt ggtcggttca tcgagtaaaa
    20401 gtaaatcggc atcggctgct aaaattttgg ctaaagcgac tttaactcgt tcgcctccgc
    20461 taagttctgt taaccgtgct tcctgacggt gtggaatccc taagccttgc aagatcgctg
    20521 caatccggtc ttctaatacg taaccgccta gttcttcaaa gcgatgctgc agctcaccgt
    20581 aacgggccag acacttctct aagtttgtat ctggttctgt catcaatgct tcgtagcgac
    20641 tcatttgctg tttgaggtcc tgtatctcag cggcagaatg gagcaagtag tggttgacgg
    20701 tcgcttcgga aactgtaagt tcttgttcga ccgtcccgat ctttaagcct tttttgcgac
    20761 tgataacacc gctgtcgaca ccttctctgc cggtcaagat ttgtaaaaag gttgatttgc
    20821 cagttccgtt gataccgact aaggcaattt tttcaccagc ttcaatttga caggataaat
    20881 cctcaaataa tggattcccg ccgattgttt ttttaataga aattgcttgt atgatcatga
    20941 tctctctcct tgagaccact caaaaaggcc atggttatcc atggcctgtg ttatttaaac
    21001 tcagattatc atgagaaaaa taccttgaga tggccattga ttttacgtga attcaactgt
    21061 tttggacaaa cttgtcccgt caacagcaac tgtaaaacca attgcatggc taaatgcgat
    21121 atggtatcgt tggtaatttt cattacttgt tattttagtc atgcgcatca tagtattttc
    21181 cctccttcgt gtagttgtct tttccagtgt acctgaaagt gtcagaaagt tcaactagcg
    21241 ctgacattta cttaatattt atcgatgtgt aattctgtca gagagctttt tttatctgac
    21301 tgagggtaat gtctcgtgga ccacatcatt gtattctgag ctaaaccagc gttcaggcga
    21361 aatcatatgg tgtttgataa aaaactttgt ctacatgact tccttttgcc atttgcttaa
    21421 taccgttgca ttaaacagct cttctctttg gaaatctcac caacatcgcg ttcaggagta
    21481 tcgttgtggt tgcaatggca agcggtagcc tttcatcaat tcttgaatga tttaccgaat
    21541 gtagcacttg ccttctcgcg gtacccgaac ccgaaataaa attttagttt ggcgataaaa
    21601 tcggtatccg ccatcgatac ttcatatttt ttcgtatcag attcatcgga aacttccaca
    21661 ttgacgaacc aataagcttt ggcatgctta ggtcgtttta aaccacacca gggcgactaa
    21721 tcgccctggt gttcactttt ttttatttta cttgaactca ttgactgttt tccctttatt
    21781 ttaaacatta taaagaccga tgagtgcttg ccgcgtcttt tgtttttatc acggtttttt
    21841 gtgcaaaaaa aactgccgat agttggcagg tcgtctacgt gttaagcttt ggtgtggtct
    21901 ttagctggca attggttttc taaatcagtt cggacaacaa gcacatcgca agctgcgttt
    21961 ctaataacat attcagaaac agaaccaata aataaacgtt ctacagcatt caatcctgtg
    22021 gctcctaaca taatcaaatc tacttgatta tcttctggga tttctctggc aataatttgt
    22081 tttggtgagc catattctac gacagaggtt acattattca agccagcttt cttcgcattg
    22141 ctttcataat ctgccaatgt ttgctttgcc atttcagtag cttgttctgc aagcattcca
    22201 tcaaaggatg aaacggtttg gaaagaacgt gtgtcaatga catgtgctaa tagaagttct
    22261 ccgttatttc ggatagctac attgaccgcc tttttaaaag ctaattcagc ttcatcagaa
    22321 ccatctacgg caaccatgat ttttttgtat tgttgtaaca taagcattca ctcctcaagt
    22381 caatcgtatt gtactctttt attgtagaac actttaggga aaaagagaat tatttactgc
    22441 tttggttcag caattttttt tgcaaatcgg atactgaagg tggcagaaac aataattacg
    22501 atcagtaaaa agaccaaggc aataaagcgt tgattgaaaa aagctgttaa aatacccatt
    22561 acgcctaaaa cagcatatgc agctccgtaa aattgtagga atccttgatt tttatcattt
    22621 ttgttgatca atacaaaaaa gatatcctgt ttattaaata aataatagct aacgaataat
    22681 aatactaaag tggcgataat cattaaaatc ctaatcatga acaaatcctc caaaataagc
    22741 aacaaataga aaaagcaatt tgccaagagc aaattgcttt attctttaaa agatggtaat
    22801 ctccgttaat gccgttgttt gtcctgtagt attgatcccg cgaattcaag caggtgggtc
    22861 ccatggtcgt agcttcgaac taccaaatga taaggcatgt taccagcagt gacacaagac
    22921 gggtcccaag atatcaataa aaatgttcgg tcaacacaca aagaggacaa ttcgaacatc
    22981 acagatttcc cacctctata gtagcatatg aatttaaaaa gagcaatgag agagctcgca
    23041 gaaacagaac cgttttcttg tctagttttt tggaggatgc tttttccact cttttatacg
    23101 gtaatactgt tgacccagag cctgttcata tttccctgta tcttttggct gataatactg
    23161 agcattcttc agtttatctg gtaaatattg ctgattgacc caagcctgat caaaatgatg
    23221 aggatactga taacccacgc cacggttgag tgatttcgca cctttgtaat ggctatctct
    23281 caaatgatca ggaacatctc cagctttgcc ctctcgaata tcagcaagtg ctgcatctaa
    23341 tgccatgtag gcagaattgg attttggcga caagcaaaga tcgacgacga cgtctgctag
    23401 tgggatacgt gcctcaggca atcccaattt ttcagcagct aatacagcgt tcactgtacg
    23461 agccgcagca gcaggattac ctaaacctat atcttcataa ccgataacca tcagacgccg
    23521 gcaaatactt gccagatcac ctgcctcaac taaacgggcg agataatgaa gtgcagcatc
    23581 cacatcactt ccacgaatcg atttttgaaa agccgaaata acgtcataat gagcatctcc
    23641 atttttgtca tgggtaagag cttttctttg gacacattct tcaatgatcg acagtgtaag
    23701 atgaatcctt ccttctttat ctgaaggagt cgatagtgta gccagttcca gcccattcaa
    23761 tgcactccgc aaatcgccgt tcgttgcacg cgaaaggtga agaagagcat cttcgtctaa
    23821 ctggattgct tgttgtccaa ggccgcgttc tttatccctt aaagcatgtt cgactgccaa
    23881 ctggatgtct tgttctgtta atggtttgac ttcaaagatc tgtgtacggc tgcggatcgc
    23941 tggattgatg gtaatataag gattctcagt agtcgctccg atcaagatga ttcggccact
    24001 ttctaaatga ggaagcagaa aatcctgttt tgttttatct aatcggtgta cttcatccaa
    24061 taagaggatc actgtaccac tcatttttgc ttcttccgct acaacttgta gatccttttt
    24121 tgaatctgta gcggcgttta gcatgcgaaa tgcataatta gtagagccgg cgatcgcact
    24181 tgcgatactc gtttttccag taccaggagg tccatataga atcatggaag agagcatgcg
    24241 agcatcgacc atgcggcgta tgatttttcc ttccccgact agatgctgct gaccaacgac
    24301 ttcatcgatc gttcgaggtc tcatgcgata agccaatggc tgttgcatag gattttcctc
    24361 cttcttgttt ttacgtccct taattatatc atatttagca agatgcctag aaagaacagc
    24421 cttctgttgt tttgttttca tagcttatcc aactaatatt gtgtatgata aagtaggtga
    24481 gaaaaatgac ggataaacaa aagatagatg aatttttttg gaataaaacg acgccagaag
    24541 tggcacggga tttattgggg atgtatttgg agcatgacac accagaagga agattagctg
    24601 gatatattgt agacgcggaa gcatacctag gtccagaaga cgaggccgcc catagctatg
    24661 gtttaagaag aacaccgcgt gtgcgggcaa tgtatgaaaa accaggtacg atctaccttt
    24721 acacgatgca tactcatcgg attttgaaca tcattactca gccagaaggg attcctcaag
    24781 gagtaatgat acgtgcaatc gaacccgccg caatgattga tcaaatgagt aaaaatcgtg
    24841 gcggaaaaac tggtccagat atcagcaacg gtccgggaaa attagtggaa gcattagcaa
    24901 ttcctcaaga actttatggt cagtctatag cagatagttc gttacgctta gtttttgaga
    24961 aaaaaaagac accaaaaaag atcaatgctt tgcctagaat cggtatccca aacaaaggtg
    25021 tatggacaga aaagccgttg cgttttgttg tgtcgggaaa tccttatatc tctttacaaa
    25081 gaaaaaacca aatcgagaaa gattggggtt ggaggaaaga aaatgaaaaa gaaggatcta
    25141 ttaacatatt tagatgaaat aattgaaaaa caagtgacag actatgatgt agctttagat
    25201 tgggatacaa aaaatcatac gatcgagatc gttgttcgct tgtttgcaga aaataaagca
    25261 caatttgaga ttgatgatgc agaagggatc gtttcgcaag aaccaattat cgaatttgaa
    25321 gacggcgtac ttctttttaa tcctcagaaa tctgtttttg atgaacgcaa ttatctagca
    25381 gtaatccctt atgaaggaaa aaaagggttg gcaaaatcag tagctgattc tttagtggaa
    25441 tatctaaatg aagtattagc tcaagggcaa agcgatttgc ttgatttttt ggatgaagac
    25501 gatgacgaag ctgtattcga gcttcactgg gataatcagc aactagcaga attagtagaa
    25561 gtaaaacagc aaaatgaagc agatacttat ttaccgtacc caagttatta aggagtgaca
    25621 gatatgaaat ggaatgaagt aaaagtagaa actgaaagtg aagcagtcga agcagttgct
    25681 aatattttga tggaggcagg ggcgagtggt gtcgcgattg aagattcttt agatgttgaa
    25741 cattttcagt cggatcaata tggtgagatt ttagataaaa atgattttac atcgctgaaa
    25801 gaaggcgcac tagtcatggc ttattttccg gaaacgactt tcttaccgga aatcttgcct
    25861 tttatcaaag atcgtatcac acaattacct gagtttggtc tagctattgg aaaaaataaa
    25921 gtaacagtta gtgaagtaga ggaaagcaac tgggcaacag cctggaagaa atactatcat
    25981 ccggtgcgta ttacgcgaat gctgacggtt gcgccaagct gggaaaacta ccagacagaa
    26041 gatcctctag aaaaaattat cacactagat cctggtatgg catttggcac aggcactcat
    26101 cccacgactt ccttgacatt gacagcacta gaaacagtct tgcgtggtgg tgaaacattg
    26161 ctagatgtgg gaactgggtc gggcgtgcta agtattgcag caaaatatct tggtgcaaaa
    26221 gatgtctatg cctttgattt agacgaagta gccgttcgtt cggcgaaaga aaatatggat
    26281 atgaatgaaa tagccaaaga tgtccatgta tcagctaacg atcttttgac aggaatcgag
    26341 attgaaagtg atgtaatcgt tgcgaacatt ttagcagata tcatcttatt gatgatccca
    26401 gatgcttggc gtttactgaa gcaaacaggc acgctgatcg tttcaggaat cattgaagag
    26461 aagaagcaaa tggttctgga tgccatgtta gaacaagggt ttatcgtgga tcaaattttc
    26521 cagcaaaaag actggtatgc aattattttg aaaaaaccag aggtggaata gatgcagcgg
    26581 tattttttaa aagaatcata tgaatcaaaa gactattttg ttttagaagg ggaaaactat
    26641 caccacattg tacgggttat gaggatggaa cccgaggatg aagttttttt ggcattcaat
    26701 gatcaaatag ccattgttgc agaaatcacg gagattactg aagaggctgt ttatctgaaa
    26761 gaaattaaaa aagaagaaaa tcaaaaggaa ctgccaagtt cgattaccat agcgagtggc
    26821 tatcctaaag gggaaaagct ggattggatc gttcagaaat ccactgaatt aggagcgcaa
    26881 gcttttcttg gtttcccggc atcagcctct gtcgttaaat gggatgcaaa aaaacgtatg
    26941 aaacgacaac aacggcttga aaagattgct caagaggcag cggaacaatc ccatcggcag
    27001 atcgtgccat cagtagagtt attggaaacg aaacaagatt tatttgagcg gctagatgat
    27061 tatgacgtgg tcttggttgc ttatgaggaa tcagcaaagc aaggcgaaaa atcaaatgca
    27121 gccaaaattc ttagtgaaac aaaaccagat agtcgaatct tagctgtctt tggaccagaa
    27181 ggtggattat cccgcgatga agtcgattat tttgtagaac acaaagggat actttgtgga
    27241 ttaggaccaa gaatccttcg tacagaaaca gcacctcttt atttattaag tgctgctagc
    27301 tatcaatggg aattaatgaa tccttctttc agctaacgtt cttcattaat aaaagagtat
    27361 cgacagaaag aattgaaaaa atagtcaaca atttgtataa tagaaacaat tatttataca
    27421 atggatacga aaaatcgttt gaaaaagctt ttaaaaagcg aacatttccg taaaaaaacg
    27481 tataactgca cggattttgg gacaattatc aaacagatat ttgtcccgat tcccgtttta
    27541 ccgaaaaaag aaagggggat cgattcctgt gacaaaagaa gaaattttaa cgggacccgg
    27601 tgtcataaag ctcgttagtc aatacatggg tcccgaacat gtagcatttg ttgagaaagc
    27661 atgcgaatac gcgactgctg cacatgacgg acagtttaga aaatcaggcg aaccctacat
    27721 tattcatcct atccaagtcg caggtatatt agcagattta aaaatggatc cccatacagt
    27781 ggctacaggc ttcttacatg atgttgttga agatacagaa atcactttag aagatctgag
    27841 agaagaattt ggcgatgacg ttgctatgtt agtggacggc gtaaccaaat tagggaaaat
    27901 aaaatataaa tcccacgaag agcagctagc agaaaaccac cgaaagatgc tgctagcaat
    27961 ggctcaagat ttacgagtta tcatggtcaa attagctgat agactacata acatgcgtac
    28021 gttgaagcac ctgcgagaag ataagcaaag aaggatcgct caggaaactt tagaaatcta
    28081 tgcgcctctt gcacatcgtc tagggatcag ccggatcaaa tgggaattag aagatacagc
    28141 acttcgttat ctaaatccaa aacagtatta ccgtatcgtc cacttgatgc agacgaagag
    28201 agaagaacgt gaaaaatacg taagtggtac tgttgaagat attcgaatag cgacagaaga
    28261 gctggggatt tttgcagaaa tctatggacg gccaaaacac atttattcaa tctatcgtaa
    28321 aatgaaagat cagaaaaagc agttcaacga aatttatgac ttgctagcta ttcgagtgat
    28381 cgtagattca atcaaggatt gttatgctgt attaggagca atccacacaa aatggaaacc
    28441 aatgcccggc agatttaagg attatattgc catgccaaaa gctaatatgt accaatcttt
    28501 gcatactacc gtgatcggtc cggcaggaaa tccggtagaa attcaaatcc gaacacagga
    28561 aatgcatgaa atcgctgaat tcggggttgc tgcacactgg gcctataaag aaggaaaaaa
    28621 tgaaaaagta gaaccagatg gtatgacgaa acaattaagc tggttccatg agatactcga
    28681 acttcaagac gaaagctatg atgcttctga atttatggaa ggcgtaaaag gagatatctt
    28741 tagtgataaa gtctacgtct tcacaccaaa aggagacgtt actgagttac caaaaggatc
    28801 cggaccattg gactttgcat acagtatcca tacagatatc ggtaacaaaa ccactggtgc
    28861 aaaagtaaat ggcaaaatgg tgcagcttga ttacaaattg aaaaacggag atatcattga
    28921 gatcatgact tctccaaatt catttggccc aagtcgcgac tggttgaaat tagttgctac
    28981 tagcaaagca agaaataaga tcaaacgttt cttcaaagcc caagatcgag aagaaaatgt
    29041 gatcaaaggc cacgaatccg tggtcaaatg tattacagat ctaggattta cgcctaaaga
    29101 tattttgacg aagaacaaac tgcaagaagc actcgatcgt tttaattatc aaacagaaga
    29161 tgatctctat gcagctgtag ggtatggaga agttagcccc ttgacgatgg ccaatcgtct
    29221 gactgaaaaa gaacgtaaag aacaaaaaat cgagcagcaa aagcaagaag cagaagaaat
    29281 catgaatcag ccgaaaaaag aacctgacaa aatgaaagta cgtcatgaag gtggcgttgt
    29341 cattcaaggg gtagaaaact tactaattcg tatcagtcgc tgctgtaatc cgattcctgg
    29401 tgatgatatc gttggttata tcactaaagg cagagggata tccattcatc gtcgagattg
    29461 tccgaatgtt cagcctgaca aaccaaatgt agcagaacgt ttgattgaag tcgaatggga
    29521 agatacatcg aatacacgaa aagagtatga tgcagatttg gaaatttacg gctataatcg
    29581 ttcaggctta ttgaatgatg tacttcaaac agtcaatgcg ctaacgaaaa atctcaacag
    29641 cgttgaagca cggacgaata aagataaaat ggcgacgatc catttgacgg ttggtatcca
    29701 gaatttatcc cacctaaaga gtatcgtgga taaaatcaaa gcagtacctg atgtctacag
    29761 tgtacgccgg acgaatggat aggaggacag atatgagagc agttgttcaa cgggtaagta
    29821 aagctgctgt gtcagtcgat cagcaagaaa ttggaaaaat tggctacggc ctacttgttt
    29881 tattaggcat tcatgaaacc gatacgcaaa aagatgtgga ctatctggta aaaaaaattg
    29941 cacaaatgcg tatttttgaa gatgaacaag gaaaaatgaa tattagtgta gaagatgtgg
    30001 gaggacaaat tttaagtatc tctcaattca cactgtttgc ggatacgaaa aaaggaaatc
    30061 gaccaagctt tgtggaagcc gcacgaccag aaacagctat tcctttatac gaatcattca
    30121 atgaaggaat cagaagtcgc ggcatcacag tcgctaccgg acaattcgga gcagatatgt
    30181 cggtttctct cgtcaatgat ggacctgtaa cgatcattat tgattctaaa aacaaataaa
    30241 gtaatctggt tttcactagt aaaaactatg aaacacgtcg cttaagttag actgataaac
    30301 tctaacttaa gtggcttttt tgtctgcaaa aagcgcttct tttgtcagca gtcggaaaaa
    30361 aagttaagct taaattatac aagttatttt ttataggagg ttgctgaaga tgaaagaagt
    30421 accagtagta gatatcaaaa tcacagattt aaaaaaattg tatcaaaaag gatacaacat
    30481 gttggaggag ttacgccatg aagcggacgc tcccgtcgtc aaagcaaaga ttttcaataa
    30541 agaagctatc acgatctacg gatcatcagc agcaaaagta ttttatgatc caaggaattt
    30601 caaacgaaaa ggtgctatgc caaaattagt attgaagaca ctttttggtc aaggtggagt
    30661 acagactttg gatggagctg ctcatcacca tcggaaaaat atttttatgg atctaatgac
    30721 accggaaaga atggaagatt atcatcgtat cttagataag aatctgacac aagcacttga
    30781 agcacagcat ggtcaatttg aactatttga tttaagtaag atggttttct ttacatctat
    30841 ttgcgagtgg gcaggtatca atttatcagc gatttctaaa gatgaggtcg aaaaattagc
    30901 agaatatcaa atctctatga tcagcggaac gtttacttct ccgattgatc acataaaagg
    30961 tgtcgaaaat cgcaaaaaat cagaaaaatg ggcgcaaggc ttgattgaag aagctcgaca
    31021 aaatcctgtg gctggaaaag aaaatgtggc attatatgct tttgccaatg ccacagattt
    31081 agatggtcag cttttacctc tggaagtggc agctgttgaa ttattgaaca tcatccgtcc
    31141 aacggttgct ctaactgtgt gggcggcatt gatgggacat gcacttttca gtagaccaga
    31201 tttgtatcag caattaaaaa atgacttttc gacgttacaa gatccgttta ttcaagaaat
    31261 gagacgctat tatcctttct tcccaatgct acctgcgatt tcgctgaaag aagtagaagt
    31321 agatggttat cgtataccag aaggcagttg ggttatttta gatttatatg gaacagacca
    31381 tgatgaacga acagttgaag cgccagattc ctttatgatc aaacggtatg tagggaaagc
    31441 aaaagatatt tcctataaag aagaatatga gatgattgca caaggcgggg gaaattttcg
    31501 acaaatgcat cgctgtgcag gagaatggat cactcttcat agtctacgtg tgttttccga
    31561 tcagctagta aataagtttg agttctctgt acctgaacaa gattggacga ttccattcaa
    31621 tcagtttccg acttatccga atagccgagc gctactttac aagaattaga ttagcataaa
    31681 cttatcgaaa aaaaggaaga tcaaacagtg ctgatcttcc ttttttcata ttactataaa
    31741 ttgataaggt caattgatca aaatccacga aatgcttcat caatggtttg ataatcttca
    31801 ttggataaat gcaaatctaa agcttggaca ttgtcagata cttgctctgg acgcttagct
    31861 ccaggtataa ctacagacac tcgtggattc ttcatgtacc aagctaaaac aagttgcgca
    31921 ggagtggctt ggtatctttt agcaatgggg cgaagtacat cgacagcggt taaaatttct
    31981 ctgaaacgtt ctccttggaa atctggattt ctttttcgag gatctccttc accaaattgt
    32041 ttttcctcac caagttcata ttttcctgtc agcaaaccgg aagcaagagg aaaataggga
    32101 acaaaactga ttttgttttt ttcaagataa gggaataatt ctttttcagc ctgccgatgg
    32161 atcaaactgt atttatcttc tactacatcg acatatccat ctgcattcgc ttctttcaat
    32221 tgttctaacg tgaagtttga cacaccgact gctcggattt ttcctgcttc ttttaattcg
    32281 tgcaatgtag caactgattc attttttggt gtgctttcat ccggaaagtg gatataaaaa
    32341 atgtcgatgt aatcagtttg cagacgtttt agtgcatctt ccaccgattg tttgagaaac
    32401 tcaggagaat tatcaaatgt tcgcccttta tttggtacat gagcagcttt tgtagcgatg
    32461 acgacacgtg agcgatcata ttctttttcc ttcagtacct cgccaatcag ttcttcggaa
    32521 cggccgtttc cgtaagcata agcggtatcg atcaaattga tcccgctatt caaagctgta
    32581 cgaactactt gttttcctgt ctcatcttcc aaaccgtcaa aaagattatg tcctccaacg
    32641 gcatttgctc ctaatccaag ctgttctgcg tagacttgtg aatgtccgat tcttatttct
    32701 gccatagctg attcctcctt tttgtctagt ctaaaacaaa ataccaaaaa gctgaactaa
    32761 aacgtttttg tgaggggaaa tagtcagata tctcttgact ttgagactgg tttttagtag
    32821 gttataagta atatcgaatg tcaatgaaag agcgagtaag tttttgaaac tttacaagag
    32881 aggatcatcg aggctgaaaa tgatctaaag tttcattagc ctaaagacac tctggagatg
    32941 gacgagtgaa aagccgtccc gcgtacgagc gttattcgta agaggaaaga gaaatctttc
    33001 aaaattaggt ggtaccgtgt atgaatttca ttcacccttg ttcatgatga acaagggtga
    33061 tttttttatt tacatagcta agaaagggag agaaaactat gagtttccaa agaccaaaag
    33121 gaacaaatga tattttgcca ggcgaatcag aaaaatggca gtttgtcgaa gaaacagcac
    33181 gtttattatt cagagattat cagtataatg aaatgcgtac accgattttt gaacactatg
    33241 aagtgatatc ccgaagtgta ggggatacaa cggatatcgt ttcaaaagag atgtatgatt
    33301 tttatgacaa aggggaacgc catgtgacgc ttcgaccaga aggtacagca ccaatcgttc
    33361 gttcttttgt tgagcataaa ttgttcggcc cagagtatgc gaaaccgttc aaaacttttt
    33421 atatgggacc aatgtttcgc tacgaacgcc ctcaaaaagg acggttacgt caattccatc
    33481 aaatcggagt agaagcattt ggatcagaaa accctgcaac tgatgtggag agcatggtga
    33541 tggcacttga tttcttcaaa cagttgggga tcacgcaaat ccggctagtc atcaattcat
    33601 taggtgataa agaaacgcgt caagcctatc gtcaagcatt gatcgactat cttttacctt
    33661 ttgaatcaga gctgagtgaa gactctaaac gccgccttca tgaaaatcca ttacgcgtat
    33721 tggacagtaa agacaaacga gatcaaaaat ttgtggcaga agctccatct atcttagatt
    33781 atctaagcga gcctgctcaa aaacattttg ataccgttgt ttccatgctg gatgcattgg
    33841 ctgttcctta tgaaatcgac agtaatatgg ttcgcggact agattattac acacatacga
    33901 tttttgaaat catgagtgac gcaccaaaaa tgggggcaca agcaacgatt tgcgcaggcg
    33961 gacgttatga caatctagtg gaagaactag gtggtccttc aacacctggc ttcggatttg
    34021 caatgggtat cgagcgatta ctcttgacta tggaggcaga ggaagtagtt atccctgcat
    34081 ttaatgagtt ggatgcttac gtggtcgcac tcggagacga aacaaatatc gaagcattga
    34141 aagtggtcca agcgattcga aactttggtt tttcagctga ccgcgacttc atgaaccgta
    34201 aagcaaaagc gcaatttaag acagcagata aagcgaatgc caaattagta ttggtgatcg
    34261 gtgaagatga gcttgctagt caaacgatta atgtcaaagc aatggcaagc cgaaaagaaa
    34321 aaagttttcc attagaagag atatatgaac attttgatga agtctatgat gaaatgactt
    34381 tattcgagta ggaggaaaaa aataaatggc acaaagaacg atctattgcg gattagtttc
    34441 cgaacaacaa ttagaacaaa cagttacatt aaaaggatgg gtacaaaaaa gacgagacct
    34501 tgggggagta atctttatcg acctacgtga tcgtgaagga atcgttcaaa tcgtctttaa
    34561 tcctgaaatc aacaaagaag catgggaaat cgcggataaa tgtcgcagtg aatacgtgat
    34621 cgaagtgact ggggaagtta gaagacgcgg agaaaatgca gtaaatccta aaatggccac
    34681 tggtgaattc gaagtgattg caagtgaaat cgtgattttg aatcaagcaa aaaccccgcc
    34741 atttcaaatt gaagatgatc aagcaatcag tgacgaaata cgtttgaaat atcgttattt
    34801 ggatctgcgt cgtccaaaaa tgactaaaaa catcaaatta agaaatgcga caacaaaagc
    34861 gattcgtcat tatttagatg ataatgactt tctcgatatc gaaacacctt acctagggaa
    34921 atctacacca gaaggcgcaa gagactacct agttccttca cgtgtccatg ctggacattt
    34981 ctatgcgttg ccgcaatctc ctcaattgtt taaacaattg ctgatgaatg ctggattcga
    35041 ccgttattat caaatcgttc gttgtttccg cgatgaagat ttacgtggag accgtcagcc
    35101 tgaatttacg caagtagata ttgaaacaac gttcttaaca gcagaagaaa tccaaactta
    35161 cacagaggga ttgattgcga aggtaatgaa agaagttcgc ggtatcgaag tcacattgcc
    35221 atttcctcgt atgacctatg atgaagcaat ggctcgctac ggcagcgaca aaccggatac
    35281 acgttttgat atggaattga tcgatctaag cgatacagtc aaagaagtag aattcaaagt
    35341 cttccaaatg gctcttgaaa acggcggtgt cgtgaaagca ttgaatgcta aaggcgctgc
    35401 agatcgctac tcacgaaaag acatggatca gttaggtcaa tatgtaggac aattcggagc
    35461 taaggggctt gcatggctga aagtagaaga agacggattg aaaggtccaa ttgctaaatt
    35521 catgggagaa gcaacagaag caatcattaa agcaacagat gcaaaaccag gtgacttgct
    35581 gatgtttggc gcagataaaa gtgaaatcgt cgctgctgca ttaggtgcta tccggacacg
    35641 tttaggaaaa gaattaggac taattgatga atcgaaattc aatttcttat gggtaactga
    35701 ttggccacaa tttgaatttt cagaagaaga aggtcgatat gtctctgctc accatccatt
    35761 cacgatgcca aaagaagaag atatcccgtt attggctgaa gaccctgcaa aagtctatgc
    35821 agaagcttat gatatcgtct tgaacggtta tgaactaggc ggaggttcta tccgtattca
    35881 tactcgtgag cttcaagaaa aagtactaga aactctaggc tttacaaaag aacaaatgga
    35941 agaacaattt ggtttcttgt taacagcttt agattacgga ttccctccac atggcggtat
    36001 tgcattagga ttggaccgtt ttgcaatgtt attagccgga gaagataata ttcgtgaagt
    36061 cattgccttt cctaaaaacg gaaaagcagc agatgtgatg agtgatgcac caagtatggt
    36121 ttcgccttta caattgttcg aattgaatat tgatgtaacg aatatagaag aatagccgat
    36181 aaaaaggtaa aaaggatttg tcgaatacgc ttcttttgac aaccttgatt taattgaaaa
    36241 acactttata atgaagccat aacatataaa gcggggcttt tccatgaaaa aaacattcga
    36301 aaattgggct tatcatgatt acacgaccaa gctttcggta tctatcgttt atgcgttgat
    36361 cgcatcagtc gcattgaatt tcttttatca gccaggacat atttattcaa gtggtattac
    36421 tggtctagct cagattttga cgactttgtc aaaatcactg ataggattcg aagtaccggt
    36481 atcaacaact ttgtatttac tgaatatacc tttgtttgta ttggcgtggt taaaaatcgg
    36541 gaagaagttt acaattttca cttttatcac agtcactttg acctcccttt gtatccatat
    36601 tttaccggaa acagtcctat ctaccgatcc aatcatctgt gcgttgtttg ggggggctgt
    36661 gatgggacta ggtattgggc tagcgttgag aaacggccta tcatcgggcg gtctagatat
    36721 cgtgagtatc acgattcgta aaaaaacagg aaagtcgatt ggttctattt cgatctactt
    36781 caatgcattg atcgtttttg ttgccggaat tctatttggc tggcaatata tgttttacag
    36841 tacgttgtct attttcgtca gtggaaaagt aactgatgtc gtttatacta aacaaaagaa
    36901 gatgcaggta atgatcgtaa cgaaacatcc agaaaaagta gtaaatgaaa tccaaaactg
    36961 tatgcgtaga gggatcacga tcatcaatga agcagaaggt gcttataagc atgataagca
    37021 gacagtattg atgacgatcg ttacacgata cgaactgcct ctacttagag aagcaatgaa
    37081 agcttctgat ccacaagctt ttgtaagtat tgcagaaaac gtccagatac ttggtcgttt
    37141 ctatgaggat ggcatttaga tcgagtttga caaaaattca tgaaatactg ctgtagacag
    37201 acgttcgttt gtctgcagtt tttttctacc atagtagaat cagcaaagaa aaactgcttt
    37261 tttatttgaa attttcttaa agttgaaaaa aagcatgttt atttttttaa atttatggga
    37321 tgatagaaaa aaatagtaga gaaaggacac gattctactt gctattttca cttaataccc
    37381 gtatattacg aatgaaaaaa gtttatgaaa agttggaata ttgaatggaa aaacaaaaaa
    37441 ggttaaaaaa cagccgctac atcactgggt ttgatgggct ccgttcatta gctgtggtgg
    37501 gtgttatctt ctatcacctt ctgccaacta gtatgaaagg cggctatttg ggcgttccga
    37561 tttttttcgt cgtatctggt tatttgatca cggatctgct gcggcaggaa tgggaacaaa
    37621 atggaaaaat caatatttgg caattttatg tcagaagaat gaagagactc tatccggcac
    37681 ttgtttttat gcttgtgaca gcttcggctt atatcacttt gtttcagcga gggctgttaa
    37741 acaatcttcg aggagttgtg gtcagcagct tactgtatgt caacaactgg tggcaaatca
    37801 ataacggttt atcttatttc gatcgatttg ctaatgagtc accgtttacg catatctggt
    37861 ctttagcagt agaaggtcaa aactatttgg tttggccgat cttgtttgta ttgttgatgg
    37921 tatttgttcg taaaagaaaa tggatctttt atactgtgtt aggtacatcc ttcctctccg
    37981 ccatcttgat gatgatcttg tatacaccag gaggcgatcc aacccgtgtt tattacggta
    38041 ccgacactcg tctgttttct atatggttgg gaagtgcgct tgcgtttgtt tggccaagta
    38101 cgcgtttaaa gaaaaacatc ccgaaacaag cgaaaaatat tctgaatatc gctggaggaa
    38161 tctcgctttt ggcactgatt ctttgcttct tctttttaga tgatcattac aatttcatct
    38221 attacggagg aatgctccta gtgagtctgt tatgcgtagt gctagttgca gtgacagcac
    38281 accccggagc aagtttgaat cgatggctga caaatccagt atttacttgg attggcaaac
    38341 gaagctatgg catttatctg tatcagtttc cagttatgat tttttatgaa gcaaaggtga
    38401 ctgatattgc cgatcatgtt ttgttgcata ctttagtgga aatcacattg attctaatca
    38461 tcagtgaact ttcttatcga tatgtggagc gtccgctagc acgttttgat tatggtcgta
    38521 cattccaagt gatgaaagga tggttcagca aaccgatcat cagtaagagc aaaccatggg
    38581 ttgttccagg cacattactt gtagcaattg cactcgttgg tttcgttacg gcaccgaaaa
    38641 acgctgtaac agccgatcaa aaacaactgc aggcaaaaat cgctgaaagt aaaaaattag
    38701 cagaagaaac cagaaacaat tcagaaaaca ccactagttc ggttgaccaa gcgatccttg
    38761 ataagtacga gttgtccaag gcacaaggga ataaggcaca aaaattagaa ctaacggcat
    38821 ttggtgattc agttatgttg gatgctgcaa cggatctaca agaactttat ccaaaagtag
    38881 tggtagatgg agatgtcgga cgacaattat atgccagtgt accgtacatt gaaaaattaa
    38941 aggaacaaga tttgttgaaa gacactgttc tgatcggact aggaacgaat ggctcattct
    39001 cggaagctca gtttgatagt gtgatggatg caatcggtga tcgaaaagta tactgggtca
    39061 atgtccgagt accgacgaag cgctggcaaa atgacgtgaa tgcgatgctt gaacagatga
    39121 cgaaaaaata cgacaatatg acgctgattg attggtacag ctacagtaat gaccatagtg
    39181 actggtttta tgatgatcaa gtccatccaa atcctgaagg gatgacacat tacattcact
    39241 tagtcagcga agccttgcta ggaggaagtt caagtacgaa cagcagtgag acaaacgtga
    39301 cagaatgata gctacaaaaa acttcaggca aaaacatttg tttttgcctg aagttttttt
    39361 atatttaccg tgtttttaac attcatatag attcaggaag caaaacggtt gagtgtgtct
    39421 gtaaataacg ttctttttgt tttcgggtga gttttttgaa tgcagcttct gcttttgttg
    39481 cttcgcttct tgaagaaaat acttctgcat ggatcatctt aacaggtctt cttttttgaa
    39541 gacgagtata tttagcacca gtaccattgt tatgttcctc taatcgtcta gaaagatcgg
    39601 ttgtatatcc accataaaat gtctgatctt tacagaataa tacgtaaaaa taatgttcat
    39661 ttgccataga gcatctcacg aatctctgca gtgtattctc catccatatt gtaagtgatc
    39721 aatggtggag cgactttgaa tccgtctaat ttgccttctt tgattccttc aactaacaaa
    39781 atatttgcct cttttccagg ttttgggtaa acaaattgga tccttttagg tgcaattcga
    39841 tagtcttcca tgacctgaat gatttctaag aaccgatcag gacgatggac catagtaaag
    39901 cgtccatttg tttttagtag cttacttgaa atatcgatca cttcttctaa cgtcgtatga
    39961 atctcatgac gggcgattgc caggtgttta ttagggttcg ttttatttgt tggcagccct
    40021 ttgaaataag gtggattaca gacgactaag tcacaagaat tatgtcgaac gacagataag
    40081 ctgtctttca gatcgatggt gtggacagtc atttgttcat ttaaatggtt aagcgcgata
    40141 ctacgtcttg ccatgtctgc tagacgaggt tgaagttcga tagcgtcgat agccgcctgg
    40201 gttcgtttac ttaaaaaaag tccaactgcg ccatttcctg cacaaaggtc aacaatcttc
    40261 ccttttttag gtacacggga aaagtgagca agcagcacag catctaaaga aaaagaaaac
    40321 actttggaac tttggataat cttgatatct tctgcgtata attggtcgat tcgttcgtct
    40381 tcgtgaagca tggattccct ccaaaatgaa aaaaatagtc gtctgtatta tactattttt
    40441 attaaaaaga accaaggtgg cttgtcaaac ctctattttt tatgcatact atgaccataa
    40501 gaagggaaag gaagagacta tgttttatcg atttatgcgc ggggtagttc gagtactatt
    40561 agccttagtt aatggaaatg cccgctatga gaataaagaa gttttaccgc aagatgagaa
    40621 ttacatacta gttgctcccc atcgtacttg gtgggaccca ttatatctgg cggtagctgc
    40681 agcacccaag gaattcagct ttatggcaaa agaagaactg ttcaaaaatc cagttctgcg
    40741 atttatatta actcgatcca atgcttttcc agttaaaaga gaaaatccag gaccaagcgt
    40801 cattaaaaca cctgtaaaga ttttgaaatc gacaaatttg agtttgatca tgtttcccag
    40861 tgggacaaga catgctacag aacttaaagg cggaatggca ttgatttcca gaatgtctaa
    40921 agcgaaaatc gtgccagctg tatatcaagg tccgttgaca cttggggatc tgttcaagcg
    40981 tcagagagtg acagtgcgtt tcggcgaacc aatcgactta tcagatatca agaaaacgga
    41041 taaagaaggt atggcggaga tcgaacgacg aacacaagca gcttttgaca aattggacaa
    41101 agaagtgaac cctgatttca aatatgaagt caaaaaaaaa taaaatgata agtttatgcg
    41161 tgggcggtct tttgaatcta ctgtcctgtt cagttcagac tttttcaatg aaaacgaggg
    41221 agagacgtca agtctgccct ctttttgcgt tccaatatgt aaaatagaag acaggtgtca
    41281 tgtaatggat caaagtgtta taataattag aaggacattt tttgaggtga ttgaatgcgt
    41341 tttttacata cagccgattg gcatatcgga aaaaaactac aaggttatga cttgattgaa
    41401 gaccagcgtc atgtactatc ttctatacta gatatcgcga aagaagaaaa agtagaggca
    41461 atcgtgattg ccggagactt atatgatagg agtgtcccag ctgttgatgc cgtagaatta
    41521 tacaatgaat tgatggtcga ttggaattta aaggaaaaga tcccgatttt tgctatatca
    41581 ggaaatcacg atagcagtac gagactttca gcgggtacgc catggttcag ttattcgaac
    41641 tattttttgc gaacacagct agcagaagca tttgaaccag tcgagtttaa aaacgtacaa
    41701 tttttctttc ttccttactt tgaaccaatt gcagcgagat tatattttga agatgacaat
    41761 atccgaacga tcaagcaagc ggtagccaag gtgatagata aaatgcagga gctatttgat
    41821 tcagaaaaaa aacaagtatt gattagccat ttttttgtaa tgggaagtgt aaaatctgat
    41881 tcggaaacga aaatcgaagt aggcgggttg gattcgatcc ctggtgaact acttacaccg
    41941 tttgattatg tagcactagg acatttgcat aatcaagctg ctttgagaca ggataatgct
    42001 cgctatagcg gttccccatt gaaattctct ttatctgaga tcaaccaaaa aaaaggcgta
    42061 tggatcgttg acctgacaac aggcctggag ttgaatttta gggagatcga acccctgcat
    42121 gatattgaag aaataacagc gagtttcaaa gaattgctgg atcctgcctt ttatcagtcc
    42181 gtcaatcggg agaattacat ctatatctat ctcactgata gaacgatcat cccgaatatg
    42241 atgaaccagc ttcggcaaat atatcctcgt attttgggga ttgaacgggc aaacggtcga
    42301 gcagaacaaa caaagcgcaa acgaaaagta ctagaaatga aagatcctca aaaattagca
    42361 gaagcatttt ttagtgaaat gacggaagag cagctaactg caaaccaaga aaaatggctc
    42421 ggagaaacgt tgcaagaaat catggaaatg gaatgatcaa gaatgaaacc gaaaaaatta
    42481 caacttaaaa attttggtcc ttttatcaat gagaccgtag atttttcacg tttgacagaa
    42541 gcaccgcttt ttttgatcag cgggaagaca ggagctggca aaacgacgat ttttgatggg
    42601 atcaccttcg cactttttgg tgaaacttcc ggaagacttc gctctggaaa agagatgcgt
    42661 tctttgtttg ctacaccaga cgaagaaaca agcgtgactt tttcttttga acatcagcag
    42721 atgagctatg aaatcgaacg aaaaccagaa caagttttag caaaacgaaa aggaaacgga
    42781 acaaaaaaac aggcagccaa agtaacgttg accatttttg atgggaaagg aaaagaaata
    42841 aaacaaatca caaaacgtac agaagcagat cagttgatca gagaactgat gaatctcgat
    42901 gcgaaacagt tttctcaaat cgttttacta ccccaaggag aattccgtaa ttttttggtt
    42961 tcatcaagca gtgataaaga gactgtttta cgtcatttat tcggtactca atttttccaa
    43021 cagttcaatg agcgattaaa agaaaaagca aaacaacagc aacaaaaatt ggatcattta
    43081 gagcaagagc tgcatcttct gcaaaaaagg tttgttttag tcaaagaaga ggaaccagca
    43141 gcagggtttg aagccgtttt agcccaatgg acaaaccatc aggcaatact cgaaaaacaa
    43201 atcacggaag aaaaagaagc tctaacggaa cttcaggaaa aacaaaagca actagaaaat
    43261 tcctactatc gttttgaaag cttgaaaaaa agttatgaag aaaaaacgat attaataaac
    43321 aagcaaaagg aattagctgc tcaaaaagac gaaatagagg aaaagaaacg atggattggg
    43381 tattatgaat ttacgcaacg attagtcgaa ccaatccagc gcctacaaga aataaaagtt
    43441 gaaaaaatag aactgtcaca aaaaagtagt gagcaaacaa agaaactagc gatgactacg
    43501 cagaattatg aagagtggca aaaaaatgaa gcaaaccgcg tacgccgaca aacaaaaatc
    43561 aaagaagctg ctcaaaaact gcaaaacaaa cagatgatgg tgccaattgt cgaatcctta
    43621 cgtagtaaaa aacaagacgc agaacaatta acaatttcat tggataaaga agaattgaat
    43681 gccgagaagc tgtatcaaaa tctcgctatc tatcaggaaa gaactcgaat tcttcaagaa
    43741 aatttactgg atcaggaaaa actgcaagaa gatcgtcttg cttttggaca gttaaaatat
    43801 ctagatgaaa agcagcaaga aatagaaaaa caaaaagcaa aagacactgg gtcgctcgca
    43861 cagataagag aagaactcaa aaaagcaact gaagaaataa caaaaaatag tcagctattg
    43921 aaagaacaga cagaacaagc agaacagctg aaaagccaat gggcaaagca gcaaattgct
    43981 cgtcttcagc tttttttgcg tccaggggag ccatgtcttg tttgtggatc aacggaacac
    44041 cccatacaac aattaaaaca cgaacagcca aataaggaag atatcttaaa agtagaacaa
    44101 caactggcag aagctgagtc tgctgtagaa agaactcagc aaatcattgc caaaagtgaa
    44161 gcacatgaaa ataatcttca acaacaatta tcagatttag aacaaaaaat catggcaacg
    44221 aatatacaat tggctgaaca agaagaacac ttgcaacagg ctttgaaaaa ggcatttcct
    44281 gagataacag aacaaaaaac aaaaacaact cttgtagtaa tcgaacaatc tttagataga
    44341 cgaaaaagca agatggccaa tgcaagaata gagctggaag aaataagaca acaagcagaa
    44401 aagctcgaac aacaattagc tgtctacaaa cagcagcaga cagaaaaaaa taatcagctc
    44461 cagcaactgc aaggagaaat caaaagttta gaacaacaat tgcaagatac ggatagtaac
    44521 tgtttagaag aggagatacg acgacttgaa aaacagttag aagaagactc gaatcaacta
    44581 gctgttgagc agaaagcagg agtgcaattg ttgcaagatc ttcatacgct tcggacacag
    44641 cttgatcttc aacatgaaca aatagaaaaa atagatgaaa aaatagaaaa ggaacaagaa
    44701 agaatagcta aaaagttaga aagccagtcg tatttttcaa caattgaaga aatctgtgca
    44761 tttgcacaac agcaagcaca atataaagaa aaccaacgta cagttacttc ttatcatgaa
    44821 gagcaattag tgacagctga ccggctgcgt caattagaaa atatccagct aactgagaca
    44881 ttcccagata cagaagagat cagccaacag ttagaagaaa taagacgtat gatcgaaata
    44941 aagcaagaaa agatatacgg tttacaagaa caaaaaatta gtaatcaaaa aatctacgat
    45001 gaatgtcttt cgatttatca cacaagccag acgaaattag atgagcttac gcaattacag
    45061 cagctttcgc aaacattcaa cggagaaaat cctaagaaga ctagtttgga acggtatgtg
    45121 cttcaggttt atcttcaaga agtgttacaa gtagcaaatg atcatctcca acgtttaacg
    45181 aaaaatcgtt atcaatttga gctagcggat accattggca gttatcgagg gaaaaccgga
    45241 ttagaaatca atatctatga tgatcaggca ggtatgtctc gaagtgcgca taccctgtct
    45301 ggcggtgaga gttttattgc agcactttct ttagcattag ctcttgccga tgtgatccag
    45361 acacaagctg gtggtgtagc aatcgatgca ttgtttattg atgaaggatt tgggtcatta
    45421 gatgaagaag ctttggaaat ggcaatggaa gctttagaaa cgatcgaaag tgaaggaaga
    45481 atgatcggca tcattagtca tgtaagagaa ttgaaagaac gagtattaca acaaataaga
    45541 attgatacag aaggtagcgg acaaagcaaa atacgctatc aacttggata aaaagggaga
    45601 ttggtaacta tgaaatggag tattccagaa aaagtcattg cacgtggacg tcaatacttg
    45661 caagaaggcc gagtgttatc ggttgtccct gaccaagaag aaaacgtgtg gcatgcagaa
    45721 gtcttgggca gtgaattgta tatggttgat ttggatggta cagcaaaaga gatagattac
    45781 tgccaatgtc cctattggga ggaacataaa tattgtaagc atacagttgc tgtggaactt
    45841 tatttacggt cgaaaaatct atctcgtatc atgaaaaaag accagccaat gctagaaccg
    45901 gtaaaagcaa caagtgaggg agaaatcatt gcaaaaggtt tttctcgtct aaaaacacaa
    45961 aacaatcctc aagcggtgca acctttgatt attgaatacc aagtagaaac gattgagact
    46021 aatcagtatc acccagagct ttctattttg gcactttact tgcgtatcgg ttcattggca
    46081 tcaccgaaaa aaacatatat cgtaaaaaac atctatgatt ttttacaagc ttacacagaa
    46141 aaagaaactt acactgttaa taaacagtac cagtttcgtc tagataaacg ggcgtttcaa
    46201 aaagaagatc aaaaaatact cacgcatctt gcaggaagtg ctcagacgca tcaattatta
    46261 ggtacgaacg gcttacaagt caaaggaaaa ctagataaaa gatatctgct tctcccagta
    46321 gaacaagccc gcttcctgtt agagcagatg aatcagacta cacgtttgca tctgcagata
    46381 aatgaacaaa acgatcaagg aatacaattt tccgatcaaa acaaaccttt atcattcaaa
    46441 gtggaaaaat ttggagaaga ttatttgctg caagcattga atgattttga ttttttcttt
    46501 atgcattatc agtggggagt tatccaaggg acattttatt ctctgacgaa atatcaacaa
    46561 acggtttatc aaacttggaa acaattgatt cgccgattgg agaaaccaga agtagtgttc
    46621 aagaaaaaag aattaccgtc tttgtttaaa gagattatcc ctctgctgtc agagatcggc
    46681 gacgtagcta ttgcccctgc agtagaagag gaagtagcag atatccccgt cgaatttatt
    46741 ttctttttcc gaaaagcaaa aggaaaaatc caagctcgag tcgatttcgt ctatggcgaa
    46801 gtcatctatt cgactgaccc gaagcatgag gtctcgacag aacaggcttt tcaggtgtta
    46861 cgtgacacag tagaagagaa acgagtactt gatctattca gaatgtatcg ctaccagaaa
    46921 aatgaaactg gttatgaacg tccattgcca agcggggaag aactatatgc ctttttccgc
    46981 acagaactat ctgtcttccg tcagtttggt gaagtcagac ttggaagaaa attgcgagag
    47041 ttgtatttag atgcgcataa acatcaaccg aaaatcgaag taaccgagac cggttcatgg
    47101 ctcgatattc gattcgatgt gacagggata gaggagcaag aaatcgatca tgtcctgcaa
    47161 agcttgctga gaaatgatgc tttttataca ttagaaaatg gtcaggttct ttcttttgat
    47221 tcagaagaat ttcaacaaac aagccaagtg ttgcaacaat tacgggaatc gatacgtaca
    47281 gaagaaggaa cgatccatgt acctaaaaat caaggattga tcattcaaaa tcaattggaa
    47341 aaaagcaatg ctacttttag tgaatcgttc caaacgatgg tacaagatct cattcatcca
    47401 gaaagatacc aggcacagct tcccaaagga ttgaatgcaa cgatgagaga ttatcaaaag
    47461 caaggttttc gatggttgaa gatgttaggt cattatcagt ttggcggtat tttagccgat
    47521 gaaatgggac ttgggaaaac actacagaca attgcctttt tactttcgga aaaagaagaa
    47581 cggaaaagct tctctgcttt gatcgtagcg cctgcgagct taatttacaa ctggcaggca
    47641 gaggtgagaa aattcgcccc ttctttaagt atccaagtaa tcaatgggaa taaaaaagaa
    47701 cgtgaagaat tactggctaa agacacagat atccgagtga cttcttatgc cagcttaaga
    47761 caggatctag ctaattatca gtcgcaaaaa attgattatt tgattcttga tgaggcacaa
    47821 atggtcaaaa atagcagtac gaaaactgca caagcattga gagaattagc tgtacctcag
    47881 cggtttgcac tgagtgggac accaatcgag aataatttag aagaattgtg gtcattgttt
    47941 gcaacgatta tgccaggatt tttcccgact aaaacaaaat tcagggaact tgccgcagaa
    48001 gaaattgcgc aaatgattcg accgtttatt ttacggcgag ataaacaaac tgtgttgaaa
    48061 gacttgccgg aaaaaacaga gatgaatcta tattctgcac ttacagaaga acaaaaaact
    48121 gtttatttag cttacttgcg tcaaatgcgt gaagaaatca catcaatgga ttctgaagca
    48181 ttcaagaaaa accggatcgg gatacttgct ggtctgacaa gactgcgaca aatttgctgc
    48241 gatcctcgtc tgtttattga agattaccaa ggcggttccg gcaagctcga acaagtaaaa
    48301 gatctactgg tagctgccaa agagaacaaa cgaagagttt tgctgttttc tcaatttaca
    48361 gggatgctga ctatcttgca agaagagtta gccgagctag ggatctcgac attttattta
    48421 agaggcagta cgaaaccaca agatcggtta agtatggtag acgcatttaa tgctggcgaa
    48481 aaagatgttt ttctgatttc cttaaaggct gggggaactg gattgaatct cacgggagca
    48541 gatacggtca tcttgtatga cctttggtgg aatcctgcca ttgaagaaca agctgctgga
    48601 cgtgcacatc ggatcggaca aaaaaatgtg gtagaagtgt ggcgtatgat ctctgaagga
    48661 acgattgagg aacgcatgga ttcactccag caagaaaaac gcgaactgtt ccaaaaagtg
    48721 atccaaggta atgaagaaca gttgacgaaa ctgactgaag aagacatccg catgatttta
    48781 agtgtaggag aaatcgagga gtaagagact cgaaaaagcg ttcagtaatg ggtaagaaag
    48841 cctttctaaa gaagcgattg tttatttgat ttaaaaaata gcaaggaggc tgggattcca
    48901 ttttatccca gcttccttgc tatcattttt ttatttctat ttttaaaggt tatacaaaga
    48961 ttgtcttcat tgtctctaac agctgatcaa aagaagaaat ctcgatatcg caaattggag
    49021 tctgttcaat tttgcgttct cgatggttga accaaagcgc ttgccagtta gcactttttg
    49081 cacctagaac atcattgtcg taattatcgc caacgtataa ggtctcttct ggaagcatat
    49141 gaaattcttt ctcagctagc tgaaaaatct ctttttctgg tttttggaaa cctgttgctt
    49201 gagaaatgag catattgcgg gaaggaatcc aattgttcaa ttgaagttgc ttcagttttt
    49261 ttgtctgatg gtcagtcggc ccatttgtga taattcctaa tgggacattc ttttccttca
    49321 agaaatcgag ggtttttttg acctcctcat gcaagcagat attatcaagt tcttcctcgt
    49381 aaattttttg aaataagagg ccatcttctt ccgtaatatg agggtagtct aaatctttta
    49441 gtgattggct gatccgatgt gctcgcatat attctagtgt ccaatctcct gccattactt
    49501 ttgggaagtt ttcatcacta tgatggcgga accggatata tagtggatgc atatctgcat
    49561 ctgaaacaag cggaacgact cttttgaccg cgtttcgaaa tggctgctgc tgatcataca
    49621 tcgtatcatc aacatcaaac acaactgatt taatcaaaat tttacacgcc tttcggtttt
    49681 cattgatttt ttcacaaatc cttgactatt gtaacagaaa aaattgtcta aaaaagctga
    49741 taaataaagg tttttcttac tatatttcta tttccttgac agaataaggt ttttatattt
    49801 cagttttttt cactaaaaat cgtgaaacac gtcaataagt ttgatgaaca aattaatcat
    49861 tatggtagaa gtc
//
LOCUS       DAZUMK010000007        49485 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000007 DAZUMK010000000
VERSION     DAZUMK010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.007"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(71..790)
                     /locus_tag="ACG808_000419"
     CDS             complement(71..790)
                     /locus_tag="ACG808_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5046077.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(921..2072)
                     /locus_tag="ACG808_000420"
     CDS             complement(921..2072)
                     /locus_tag="ACG808_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFS5046078.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(2289..3029)
                     /locus_tag="ACG808_000421"
     CDS             complement(2289..3029)
                     /locus_tag="ACG808_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5046079.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(3033..3356)
                     /gene="rsfS"
                     /locus_tag="ACG808_000422"
     CDS             complement(3033..3356)
                     /gene="rsfS"
                     /locus_tag="ACG808_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFS5046080.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(3413..4006)
                     /gene="yqeK"
                     /locus_tag="ACG808_000423"
     CDS             complement(3413..4006)
                     /gene="yqeK"
                     /locus_tag="ACG808_000423"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFS5046081.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(3990..4640)
                     /locus_tag="ACG808_000424"
     CDS             complement(3990..4640)
                     /locus_tag="ACG808_000424"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFS5046082.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(4650..4961)
                     /gene="yhbY"
                     /locus_tag="ACG808_000425"
     CDS             complement(4650..4961)
                     /gene="yhbY"
                     /locus_tag="ACG808_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFS5046083.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(4977..6086)
                     /gene="yqeH"
                     /locus_tag="ACG808_000426"
     CDS             complement(4977..6086)
                     /gene="yqeH"
                     /locus_tag="ACG808_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFS5046084.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(6083..6610)
                     /locus_tag="ACG808_000427"
     CDS             complement(6083..6610)
                     /locus_tag="ACG808_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFS5046085.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(6790..7575)
                     /locus_tag="ACG808_000428"
     CDS             complement(6790..7575)
                     /locus_tag="ACG808_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFS5046086.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(7568..8437)
                     /gene="accD"
                     /locus_tag="ACG808_000429"
     CDS             complement(7568..8437)
                     /gene="accD"
                     /locus_tag="ACG808_000429"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFS5046087.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(8438..9817)
                     /locus_tag="ACG808_000430"
     CDS             complement(8438..9817)
                     /locus_tag="ACG808_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFS5046088.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(9825..10244)
                     /gene="fabZ"
                     /locus_tag="ACG808_000431"
     CDS             complement(9825..10244)
                     /gene="fabZ"
                     /locus_tag="ACG808_000431"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFS5046089.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(10269..10745)
                     /gene="accB"
                     /locus_tag="ACG808_000432"
     CDS             complement(10269..10745)
                     /gene="accB"
                     /locus_tag="ACG808_000432"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFS5046090.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVISEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(10748..11983)
                     /gene="fabF"
                     /locus_tag="ACG808_000433"
     CDS             complement(10748..11983)
                     /gene="fabF"
                     /locus_tag="ACG808_000433"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFS5046091.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(12002..12739)
                     /gene="fabG"
                     /locus_tag="ACG808_000434"
     CDS             complement(12002..12739)
                     /gene="fabG"
                     /locus_tag="ACG808_000434"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFS5046092.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(12751..13668)
                     /gene="fabD"
                     /locus_tag="ACG808_000435"
     CDS             complement(12751..13668)
                     /gene="fabD"
                     /locus_tag="ACG808_000435"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFS5046093.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEATPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(13775..13999)
                     /locus_tag="ACG808_000436"
     CDS             complement(13775..13999)
                     /locus_tag="ACG808_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFS5046094.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(14052..15014)
                     /locus_tag="ACG808_000437"
     CDS             complement(14052..15014)
                     /locus_tag="ACG808_000437"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFS5046095.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(15024..15464)
                     /locus_tag="ACG808_000438"
     CDS             complement(15024..15464)
                     /locus_tag="ACG808_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS5046096.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            15770..16735
                     /locus_tag="ACG808_000439"
     CDS             15770..16735
                     /locus_tag="ACG808_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFS5046097.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(16792..17022)
                     /locus_tag="ACG808_000440"
     CDS             complement(16792..17022)
                     /locus_tag="ACG808_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFS5046098.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(17108..17995)
                     /locus_tag="ACG808_000441"
     CDS             complement(17108..17995)
                     /locus_tag="ACG808_000441"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS5046099.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(18084..19307)
                     /locus_tag="ACG808_000442"
     CDS             complement(18084..19307)
                     /locus_tag="ACG808_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFS5046100.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(19324..20370)
                     /locus_tag="ACG808_000443"
     CDS             complement(19324..20370)
                     /locus_tag="ACG808_000443"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFS5046101.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(20534..22261)
                     /gene="ptsP"
                     /locus_tag="ACG808_000444"
     CDS             complement(20534..22261)
                     /gene="ptsP"
                     /locus_tag="ACG808_000444"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFS5046102.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(22261..22527)
                     /locus_tag="ACG808_000445"
     CDS             complement(22261..22527)
                     /locus_tag="ACG808_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFS5046103.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            23006..25240
                     /locus_tag="ACG808_000446"
     CDS             23006..25240
                     /locus_tag="ACG808_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5046104.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            25388..25708
                     /locus_tag="ACG808_000447"
     CDS             25388..25708
                     /locus_tag="ACG808_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFS5046105.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(25913..26137)
                     /locus_tag="ACG808_000448"
     CDS             complement(25913..26137)
                     /locus_tag="ACG808_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFS5046106.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(26335..27915)
                     /locus_tag="ACG808_000449"
     CDS             complement(26335..27915)
                     /locus_tag="ACG808_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFS5046107.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(28316..29692)
                     /locus_tag="ACG808_000450"
     CDS             complement(28316..29692)
                     /locus_tag="ACG808_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFS5046108.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(29820..30908)
                     /locus_tag="ACG808_000451"
     CDS             complement(29820..30908)
                     /locus_tag="ACG808_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS5046109.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            31080..31514
                     /locus_tag="ACG808_000452"
     CDS             31080..31514
                     /locus_tag="ACG808_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5046110.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(31592..32125)
                     /locus_tag="ACG808_000453"
     CDS             complement(31592..32125)
                     /locus_tag="ACG808_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFS5046111.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(32219..33397)
                     /gene="mutY"
                     /locus_tag="ACG808_000454"
     CDS             complement(32219..33397)
                     /gene="mutY"
                     /locus_tag="ACG808_000454"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFS5046112.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(33390..34187)
                     /gene="recX"
                     /locus_tag="ACG808_000455"
     CDS             complement(33390..34187)
                     /gene="recX"
                     /locus_tag="ACG808_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFS5046113.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            34294..35676
                     /gene="rlmD"
                     /locus_tag="ACG808_000456"
     CDS             34294..35676
                     /gene="rlmD"
                     /locus_tag="ACG808_000456"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFS5046114.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(35850..36428)
                     /locus_tag="ACG808_000457"
     CDS             complement(35850..36428)
                     /locus_tag="ACG808_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFS5046115.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            36750..37220
                     /locus_tag="ACG808_000458"
     CDS             36750..37220
                     /locus_tag="ACG808_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFS5046116.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(37328..38554)
                     /locus_tag="ACG808_000459"
     CDS             complement(37328..38554)
                     /locus_tag="ACG808_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFS5046117.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(39308..39508)
                     /locus_tag="ACG808_000460"
     CDS             complement(39308..39508)
                     /locus_tag="ACG808_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS5046118.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(39642..40250)
                     /locus_tag="ACG808_000461"
     CDS             complement(39642..40250)
                     /locus_tag="ACG808_000461"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFS5046119.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(40335..40805)
                     /locus_tag="ACG808_000462"
     CDS             complement(40335..40805)
                     /locus_tag="ACG808_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFS5046120.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(40853..41767)
                     /locus_tag="ACG808_000463"
     CDS             complement(40853..41767)
                     /locus_tag="ACG808_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFS5046121.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            41913..42716
                     /locus_tag="ACG808_000464"
     CDS             41913..42716
                     /locus_tag="ACG808_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFS5046122.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            42894..43841
                     /locus_tag="ACG808_000465"
     CDS             42894..43841
                     /locus_tag="ACG808_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFS5046123.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(43909..44202)
                     /locus_tag="ACG808_000466"
     CDS             complement(43909..44202)
                     /locus_tag="ACG808_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFS5046124.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(44230..44895)
                     /locus_tag="ACG808_000467"
     CDS             complement(44230..44895)
                     /locus_tag="ACG808_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFS5046125.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(45035..45667)
                     /locus_tag="ACG808_000468"
     CDS             complement(45035..45667)
                     /locus_tag="ACG808_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS5046126.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDWPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(45674..46741)
                     /gene="liaS"
                     /locus_tag="ACG808_000469"
     CDS             complement(45674..46741)
                     /gene="liaS"
                     /locus_tag="ACG808_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFS5046127.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEITKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILKLERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWEVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVETKVPLMKEA"
     gene            complement(46738..47469)
                     /gene="liaF"
                     /locus_tag="ACG808_000470"
     CDS             complement(46738..47469)
                     /gene="liaF"
                     /locus_tag="ACG808_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFS5046128.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDIEVIRI"
     gene            complement(47637..48116)
                     /gene="greA"
                     /locus_tag="ACG808_000471"
     CDS             complement(47637..48116)
                     /gene="greA"
                     /locus_tag="ACG808_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFS5046129.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(48252..49427)
                     /gene="mltG"
                     /locus_tag="ACG808_000472"
     CDS             complement(48252..49427)
                     /gene="mltG"
                     /locus_tag="ACG808_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFS5046130.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
BASE COUNT        14176 a        10457 c         8302 g        16550 t
ORIGIN      
        1 aagacaatct gttagtgaga ttgtcttgct tttcgaaaaa gactgaatac ttttcgttaa
       61 aactggttgc ttagtcttct aatgccacat tatggaacac atgctgtaca tcttctagat
      121 cttccagtgc atcaatcatt ttttcaaatg tttcttgatc ttctggagat agtgttacct
      181 cgttttgagg gatcatttgg atctcagcga ttgaaaaatc agtgattcct ttttctttta
      241 atgcttcttg tatactatgg aaatccgcta cttcaccgta aacgatgatt tgtccttctt
      301 cttccattac atcgcgaaca tcgatgtctt tttccattag gtattcaagg atctcgtcag
      361 cgtcttctcc tgcaaaagca aagattgctg tattgtcaaa catgtaagaa accgctccgg
      421 aaacacccat gttcccgcca tttttcccaa atgctgcccg tacgtctgct gctgttcgat
      481 tcacattgtt cgtcaatgta tcaacaatca ccattgagcc gcttggtcca aatccttcat
      541 agcgcaattc agagtatgtt tcatctcctg caccttttgc tttttcgatt gctcggtcaa
      601 taatatgttt tggtacatta taagttttag cgcgatcaat gacgaaacgt agtttttggt
      661 ttgcatgcgg atcaggatca cctgattttg ctgccgcgta gatttcaatc ccaaattttg
      721 cataaatccg gctattatta gcatctttag cagttttctt tgctacgata tttgcccatt
      781 tacgtcccat attttcactt cgctttctga tttgatagtt tcataacgca aaaacgtcct
      841 gttccattat acttgtgaat cacgattctg tgaagctttt tttctttctt ccttcattct
      901 tctcattttt agtcaattat ctatactcgg attgggattt gcccgaaatt ctgttccgga
      961 atattgcctc ccaattgata gatttgatca cttctaacta acagttccgc ttcttgtgcc
     1021 tctttttttc cgattcggga ataaatagaa atctcttttt tttcttttaa gaatcttctt
     1081 ccagcatcgg taaatccaag tagtcgaaga taatttttat tttgttggac aggaatctct
     1141 tgctttttga tattcagcaa gacataacaa gataatcgtt ggatccgtgt ccatgtatat
     1201 ctttttgttt ttacctttga cacaaaatcc tcaaaactat cagcagttgc agctgctgtt
     1261 ttcatgcgcg attctattcc ttcggtcatc tgatagatat ctgtcagttc cagcaaagta
     1321 gaagataaca accgataacg caaatacggc caataagatt cccaagtctg cagcgaacaa
     1381 gttgaaagat cttcttctgt ctgaggcggc acaagtccag ctattctttt tccttcttct
     1441 aacgcatggc gaatggctgt tgcactggct atcctctgat ctgtgatgat tttttcatga
     1501 tagcctgcac ttttacgtcg gatcggatgt aattccatag ggtggacata cttggcattt
     1561 tcctttgcat aacttaatcc caagatatga tttggcgaag aaaaatcaag cgtaatatcg
     1621 ggatacagac tgcgaaaaac ctcagtcatt ttttgcggat aactatacgt ttgatcagac
     1681 agttcttgaa atgcctcttc tatcctatct tgattctctt ggacaaagcg tccgaaactg
     1741 ctgtaatcaa acgaagtctc ttcttctgtt ccaaaacaca aactgtcaca gtccagtgtt
     1801 tgcaagattt ttactgcacc tttagcaaaa tagtcagctg actgaaccgc ccattcgacg
     1861 ggaagctcca ctactaaatc cacaccattt tgtaaagctt cattcgcgcg atgccattta
     1921 tcgatgatag ccggttcgcc tctttgcaaa aaattcccgc tcattacagc aatgacgacc
     1981 tctgctcctg ttatttttcg tgccatttcc acatgatagc gatgtccatt gtgaaaagga
     2041 ttgtactcta cgataatccc gcagcttttc atgcgcatcc tccttcatag ttatcgataa
     2101 aaaagaaata gagacaagta aaaaaaggca aaaatcggaa aaaatacccg aactatataa
     2161 actattctcg caaacacaat acttctgcga cgaaaagctg cactgcaaga gcaatgccta
     2221 tatcgttcgg atatataatt tatgttgaag atttattctt ttcttgttaa ctcgagcagg
     2281 caaaaccgct agtctttgta tgctacaaag aaccagcgct ttgatttctc tgatgctgct
     2341 tcgtctgtaa aatcagaaaa tgcttcgact ttcgaaaagc cgctgttttc cagcattctt
     2401 aaataacttt ccaatggata ggtacgctct tgatgtagtt catcttcacg aataaatcga
     2461 ccagaccctt cttgttcttc aacgaaaaag gtaagaaaat gttcgatgct gtgtttctcg
     2521 cttcctggat aactatccca caaaaaagca aaattatccc cttgataatg atagctatat
     2581 tctggaaaaa cctcatctac ttgataggtg gagtgtacgt caaataaaaa tcggccattt
     2641 tcttctaacg cttgatagac ttcatcaaac acttgctgta cctcttgtct atttttcata
     2701 tagcacagcg aatcagaaaa acaggtaatt gcctgatatt gtccgatttc tgataaatcg
     2761 agcatattgc cttccacaaa ttggatcgat gcgtcttctt catatgctcg ctggctggca
     2821 atcatcaaca tctcttctga aaggtctaac cctgtcactt tatatccatc ttttgcaaag
     2881 gcaaccgcta atgctccagt accgcatgcc agttccaaaa tattttttgt gtcttcttgt
     2941 aaatgacgtt tcgaaaaagc taaccattgc tgataaagtg tttcgtccat cacttcatcg
     3001 taaatgaaag caaatgtttc ataagccata tgttaatcca accattcact aagatcgact
     3061 agcggagcat ctgaccatag tttttccaga ttatagaaat ttcgttctgg cgcatggaaa
     3121 acatgtacga tcacatctcc aagatcgatc aatacccatt ttccgccttc ttttccttca
     3181 atacgtttta cttcatattt gttttcttct tctttatcaa tgatttcttc tgtaattgcg
     3241 ttgatttgac gttcgctatt tgctgaacag atcatgaaat aatctgccaa caaggaaaca
     3301 tttcgaacgt ctaaagcgat aatgtcttct gcccgttttg aatctgctgc ttcaatcgca
     3361 atttttaatt gttctttact atctatgatg atttcctcct aatatgagca gcttattttt
     3421 ttgctaccca gtaattatat gtttcgattg tttttggata aatctgttgt tcttgcgcaa
     3481 gcaaatgggc caatgtatgt tttgtttcaa atgctacagc ttcgtctaga tcagcccatg
     3541 caatggctcg tgcatcttgt accccaggaa aatctcgacc tggttcaata tagtctgcta
     3601 cataaatgat tttgtcaagg agactcatct tagcagcccc agtcgtatgc acccggattg
     3661 catgaagaat ctctgcatcg gtgattccta attcccgctc tacaaacgaa gcgccaacaa
     3721 gaccatgcca gattgcatta ttataattca ataactccgg atcaaaaccg tctcttacga
     3781 ttaccatttt gaactcctca tctggtcgtt cttttgcata atcatgtgtc aatgcagcaa
     3841 tactagcttt ttcaggagaa gcaccgtatt ttttggcaag agccacggct gtttcttcta
     3901 ctccaagcac atgcttgaag cgttgttcgc tcattctcat ctgtattttt tgcatcaggt
     3961 tttctctaga ataagcagta tattcattac tatagtccat attcatacaa cccttcattt
     4021 tgaatataat caattacttt atctggaaca aggtagcgga tagaacagcc ttctttgatc
     4081 ttttgacgta ttttcgtgga actgatgtcg atctcaggta catctaccca aattacagga
     4141 tatggagtat ccgttgtgta tcctggtcga cgtataccca caaagttgac catagaagtc
     4201 agctcatcga ttttatacca ttttggcaaa tattctacca tatccccacc gataataaag
     4261 taataatctg tatccggatt gttttgcgtc aattccttca tagtatcata ggtgtaactt
     4321 ttcccaccac gagctaattc aatcgtttca atctgcaaaa aaggattgtc ttccaccgct
     4381 aattccaaca tgttgagtcg atgttttgca tcaatcgtct gtttctcatc aacatgaggt
     4441 ggctgataag aaggcatcaa aaatacgcgg tcaagaccta aattccggcc agcttgttca
     4501 gccatcacta aatgtgcaag gtgaacggga ttgaatgttc cgcccaagat ccctacttgc
     4561 tttttcttct ctaagaattg aggcatttct tcaggaaata attgtgaacg aacaaatgtc
     4621 ttagcttgcg tattcatcat ggtcactcct tatattgctt ttacttcttt tgatagcctt
     4681 tgatactttt cttttgtgga aggtttgtat aatacaataa cacgtccaat gatttggacg
     4741 atttcgcaat ctatttcttt ttctaatact tgagctgctt cttctgcaat ctcatctgta
     4801 ttttgaagta acgatacttt gatcagttct cttttttcta aagcttcttc aatttgaacg
     4861 atcattgctg aattgatccc accttttccg atttgaaaaa tcggctgaag atgatgcgct
     4921 ttgctacgta aaaaacgctt ttgttttcct cttaaactca taattttcct tcttccttaa
     4981 atcaatgctt tgcgtttcaa tacatctaca ccctttggcg cccagcccgc aattactact
     5041 ggcgctgtta cagtgatcca gcctagaccg gcaaaaacga tgtctgtttt ttctttcaca
     5101 gaaaactcaa aacgaacaag ctccggaaat gcctcgactt catcaggacg aggaggctgt
     5161 aataatccac ctacatgttt ttgataaaat tcgtctgctt tttccagttt cgttcgatga
     5221 atatttacat catttgacac ataagcaata aatgaagcac gattgccgct gataaaatca
     5281 aatcttgcaa gtccgcccaa gaaaagtgtt tgcccttcat tcagctgata aactttaggt
     5341 ttgatttctt ttaacggtgc aatgatccgc aaatcttttt tacctaagta gtgcgccatt
     5401 tgatggcgat ggataattcc cggtgtatca atcaaaaaat gcccgtcatc taaaggaatc
     5461 tcgattttat ccaaggtcgt tcctggaaac tgcgaagttg tgattacatc ttgtactccc
     5521 gctgtatttt tgatgatttg attgataagc gtcgattttc cgacatttgt tactccgacg
     5581 atatagacat cccgtccatc gcggtattct tcgatttttt ctaatagttc cgtcatctca
     5641 tgagatttct tagcactagt taataataca tctactggac gaattcctgc ttcgtaagca
     5701 cgttccttca tccattgtgt taatttagtt tttttcaatg atttaggtaa tatatccact
     5761 ttgttcccaa ctaaaagtac tggattgtca ccgacaaaac ggtgtagtcc tgggattagt
     5821 gaaccattga agtcaaaaat atccaccaca ttgacgatca atgcatcttt ttgccctaat
     5881 tcattcagta agcgcaaaaa gtcgtcatcc gtcaatgaga catcttggat ctcattataa
     5941 tggcgtaatc tgaaacaacg ctgacaatag acttccccgg aagctaaacc tttttccaaa
     6001 gccgtttgcg gcgtgtatcc taattcaccg ggtttgtttg tttgtataac ggcaccacag
     6061 ccgatacatt gtaattcttc tgtcattcga ttccacctcg ccagcccata tcagggtgtt
     6121 ttgctaatag ataggccata attctttttt cccaaaaacg attaaattgt gtcttccaac
     6181 tatctgttgc gataatgggt tttacaagga tgctacgtat ccctgcacga ttggctccac
     6241 gtatatcagt cataatctga tcgcctacca ttactatctc atcatctgct aaatccaatt
     6301 gtttctttgc tatgttgatc cctcttgcta aaggtttcat tgctcgtgca acatacaaaa
     6361 gatcaaattt ttcaatcgct cgcgcgacac gttttgaatt attgtttgaa acaacaacaa
     6421 cagggattcc tgcatttttc atttctaaaa tccaagtaag caactcttct gttccatctg
     6481 gattattcca tgcaatcaac gtattgtcta aatcggtcaa tactgctttg atgcctagat
     6541 tctttaattg ggcaggtgta atctgataga tggcttctac catccatgtt ggtttgtaat
     6601 ttgaaaacat gctgtctcct catctgcaaa aaatgaagga gcacataaag tactcctcaa
     6661 ccccgtatta tactgcatat cagcaaaaaa aactacctct atactcgttc gtctttcaga
     6721 aaaagcattt ctttacaaaa tgaccaaaaa taaagattga tcacagaaaa atttagaaac
     6781 aaaaaacaac taatattggc ggaacctctt gtatcgtttt tccagtaatt tatgctgatc
     6841 taatcgaaca agttccgtca atctttcaat caacgctttt ttcataatgt gtgcgacttt
     6901 atatcgtggc aactgctcgc cgttgaacgt ttcaggaata actcgatcaa cgattcctaa
     6961 ctcgcgtagt tctggcgcag taattttcat caactcagct gcttctgcgg cgcgactacc
     7021 gtctttccac aaaatcgacg caaatccttc tggagaaagc acagcataaa tcgtttcttc
     7081 cagcatccag acttcatccg ctaaacctag agcaagggca ccgccacttc caccttcacc
     7141 aatgatgatg gcgatgatcg gtacctttaa atcagacatt tcgagcagat ttctggcaat
     7201 agcctctcct tcgccgcgct cttctgctcc tacaccgcaa tatgcacctg ctgtattgat
     7261 caaggtaatc accggtctat tgaacttttc cgcttgtttc atcagtcgta aagcttttcg
     7321 atagccttca ggatggggag aaccaaaatt tctgcggata ttttcctgca gatcccgtcc
     7381 tttttgaata ccgatcactg ttaccggttt gccatctaat cgagcgattc caccaacaat
     7441 cgcttcgtca tctgcaaata aacgatcacc atggcattcc tcgaaagaat cgaagatctc
     7501 ttcaaaaaaa tccaagcttg tccatcgttc tttagctctt gctaactgaa cgacatcatg
     7561 tgctgtctta tccacgatct atccatcctt tctgtgtatg caattcaaga agcgtatgga
     7621 ttttctgttt caattccatg cggggaacaa tctgatcgac aaaaccatga gataataaga
     7681 attcagcctt ttggaaatct tctggaagtt cctgcttgat cgtctgttcg atgacgcgtc
     7741 ttccagcaaa accgatcaag ctttggggtt ctgctagaat aatatcgcct tccattgcaa
     7801 aactggcagt tactcctccg gtcgtcgggt ccgttaaaac agttagataa aaaagacctt
     7861 cattgctatg ccgtttgaca gcagcagaga tttttgccat ctgcattaga gaaaagattc
     7921 cttcttgcat ccgtgcgcca cctgaagctg tgaacaagac gactggaaga ctttcctttg
     7981 ttgctcgttc gaataatctg gtgatctttt ctccgacaat cgttcccata cttcccatga
     8041 taaaattagg atccatcact ccaataatga agggaatccc ctgaatggtt gctttaccag
     8101 tgaggactgc ttcatgaagt cctgttttat cttgcatttt tttaattttt tccgtatatt
     8161 cgggaaatgc tagcggatct ttggttacaa gatccgtatc ccattcttca aaactttttt
     8221 catcaataat caaagctaga cgctgccatg caccgattcg aaaagcgtat ccgcaatgag
     8281 ggcatacttt ttcttcaccg atgtctttcg tataaaggat atgtttgcaa tttggacact
     8341 ttgcccacat gttatcagga acagatggcg agttagcagg tttgctctct ctattaggat
     8401 tgatccgaat atatttcttt ttcttaaaca gagccatcta tgcgcctcct atctccgttt
     8461 ctggtgtcca attcggtaaa aattcttctt gtaaaaatgc tgtgctgtaa tcacctgcta
     8521 tgactgcagg atgactaatc aaatccatct gaaattctgc gttcgtgatg attccttctg
     8581 tgataaattc acttaaggct cgctgcatct tcattaatgc ttcaaaacgc gtatttccat
     8641 ggacgattat ttttgcaatc attgaatcat aatatggcgg gatcgtatat cccgggtaaa
     8701 ctgcactgtc tactcgcagt cccatcccac ctgctggaag cagtaagttt tggatcttcc
     8761 ctggagaagg agcaaaatgg aaagcaggat tttctgcatt aatccggcat tcgattgcgt
     8821 gtccttggaa tactacttct tcttgacaca cattcaaagg ctcaccggct gctatctcca
     8881 cctgtttttt tactaaatcg attcctgtga ccatttctgt tactggatgt tccacttgga
     8941 ttcgagtatt catttccatg aaataaaaat cgccttcttc atccatcaga aactcgatcg
     9001 ttccggcatt ttcataattg actgctttag ctgctcgaac agcagcgtct cctagtgctt
     9061 ctctttttgt ttgtgagatc actacagaag gagattcttc cagtactttt tgattgttgc
     9121 gttgtaaaga acaatctcgc tcgcctaaat gaatgacatg accaaatcga tcacctaaga
     9181 tctgaacttc tatatggcga gcaggataaa tgattttttc caaatacata tcatcatttc
     9241 caaatgccgc tgctgcttct tgctgagctg aagaaaaatg ctgtgggagc tcttcttttg
     9301 attgaacttt ccgaatgcct ttcccgccac cacctgcagc tgctttcagc atcactggat
     9361 aaccaattct gtctgctatt tccaatgctt cttctactgt agataataca ccagtacttc
     9421 ctggaatgac tgggacattt gcttcttgca tcaattgacg cgcattgatc ttatttccca
     9481 ttgcatcgat cgttgcactt ttaggaccaa taaatgtgat attgcactct tcacacatgg
     9541 aggcaaatcg gctattttca gataaaaaac caaatcctgg atgaattgct tcggcttttg
     9601 tcacgattgc agcgctcagt acttcctgaa cattcagata tgaatcaacg gcttttgccg
     9661 ggccaatgca gattgcttca tctgctaact gtgtatgaag tgcttcggca tctgcttctg
     9721 aatatatcgc tacggtttgg atccctagtt cgcggcatgc acgaatgata cgtacagcga
     9781 tttcaccgcg gttagctatc aatactttag aaaacatatt tcacctatcc aatcataaag
     9841 gttaattccg cttcggctac ttttttaccg tctactttag cgattccttt gcctattccg
     9901 gctgaagctt tcactttcaa tatctcaact tcaaggtata aggtatctcc cggtgtaact
     9961 ttcttacgga attttgcttt gtccaatccg ccgaaataag ccgtctttcc cttgaattgt
    10021 tcaagagaaa gtaatgcaac tgctcctgct tgagccattg cttcaacaat caatacccct
    10081 ggcataacag gttcttctgg aaaatgtcct tgaaaaaagg gctcattgat cgttacattt
    10141 ttcttagcaa cgatccgttc tccttcaacc atttcttcca ctcgatcaat caaaagcatt
    10201 ggataacgat gtggaatgat ttcttttatt tcttgtatgt tcattgtttt ccctctttcc
    10261 tgctttgctt atttttgctg aatcctaaag agtggctgtc catattcgac aactgactcg
    10321 ttttctacaa ggatttctgt gatcactccg tcggcctcac tagttatctc attcatcagt
    10381 ttcatcgctt caataatgca gataacttct cctttggcaa cttgatcacc tacttttttg
    10441 aaagctggtt tgtcgggcgc tggttgcaga taaacgattc ccacgattgg cgaaacaacc
    10501 tcgattccat caaatactgc cgattctttt tctttcatta tttctggagc agttgttgct
    10561 tgttcagata tgacattgac tggctccgaa actggcaacg tttcgtttgt ttgaaccata
    10621 ttcatgcctc gacttgtttt atttttattc atatacaatt caaattgacc ttcacgcaaa
    10681 tcgaattccg ttagcgagga ttgatcaaat tgactgacta attctctgat ttcattgata
    10741 tccaaaatta ttcctcccag cgtttcatgc acagaaccgc attatgtccg ccaaacccta
    10801 atgaattact cattgcataa gtaaaacttg caggtttgct ttcgtttttg acaactgtca
    10861 agtcaatctc aggatcttgt ttttctacat tgatcgtagg tgggataaat tgatgttcta
    10921 atgccagcaa agttgccaag gcttcgatcc cgccggctgc tcccaataaa tgtccagtca
    10981 tcgatttcgt gctgctgacg taagtatctc tgaaattttc acctaatgcg atttggattg
    11041 cttttgcttc tgcctggtca ttggctggtg tacttgttcc gtgagcattg atatatccga
    11101 cttgttccgg ctgtacacca gcttcttcca tcgcaagctt cattgcttta gatgcaccag
    11161 aaccatctgg agtaggagct gtcatatggt aagcgtcaca gtttgcaccg tatccaacga
    11221 tttctccata gattttggca ccgcgagctt gtgcatgttc taaagattca agaactagaa
    11281 cgcctgctcc ttcacccatc acaaaaccat tccgattttc gtcaaacgga atcgaaccac
    11341 gattaggatc agttgattct gagagtgctg ttaaagctgc aaatccagca atccccatct
    11401 cacaaatcgt tgcttctgtt cctccagcaa gaatgacatc agaatagcca tgtttgatgt
    11461 tacggaatgc ttccccaatt gaattcgttc cgctagcaca agctgttacg atagctgtac
    11521 aaataccttt tgcacctaca cgcaaagcaa tatttccagc tgccatgttg gcaatcgcca
    11581 ttggtacgaa cattggagaa actcgttttg cacctttttc atgtagacga ataacttggt
    11641 tttcgattgt tggaagacca ccgatcccag atccgatgat gacgccaaac cggtcttgat
    11701 tcattttttc tttatctaaa ccgctcatct ccattgcttc taaagcagcg tagattccat
    11761 agatcgaaaa catatccatt cgtttgctat cttttttaat aaaatattta tcgtaaggga
    11821 aatctttgac ttcacccgct aaggtaatcc ctgtttctga agcatcaaat ttagtgatcg
    11881 gtgcgatgcc attcgtcccc tcttttaagc tattcaaaaa gctgtctgcg tcatttccaa
    11941 taggtgaagt gactccatag ccagtaatta ccactcgatt catttgtttg ctctcctttt
    12001 cttatccgtg catgaccaat ccgccatcga cattgatgac ttgtccggtg atatatggat
    12061 tttgtgctaa gaaaactgct gtttgtgcaa tatcttctac ttgtccgaaa cgctgcatcg
    12121 gaatttgctt ttccgcttgt tccttgactt tgtctgctaa aacttccgtc atatcagttg
    12181 tgatgaatcc tggcgcaatg gcattacatg taatgcctct agttgcagct tctcgtgcca
    12241 cagattttgt taatcctact acgccagctt tactagctgc ataatttgct tgaccaatat
    12301 ttccaatcaa cccagataca ctagagagat taatgatggc cccttctcgc tgcttcatca
    12361 tttttttcaa tacgtgctgc gtcatattga aggttccaat caaattgata tccagacatt
    12421 ttttgaaatc ttctgcatcc attcgcatca caagtttgtc atttgtgatt ccggcattgt
    12481 ttactaacac atggatcgat ccaagctttt cttctgcttc tttgatcatt tgtcccgctt
    12541 tctcataatc agagatgtca ccggaaactc ctacgcattt tacaccgaat gcttcgattt
    12601 cttctatctt ttcttttgga atctctccgc gaccattcaa aatgatattt gctccagctt
    12661 ttgcaaaagc taaggccatc gctttgccga ttcctcttgt actacctgtg ataaatacat
    12721 tttttccttt tacatccata acgttcctcc ttatagtatt gctattgttt catcaaatgt
    12781 ctgcttattc tcaacacgca gaactggtat tgttttatcg atttttttca taaatccgct
    12841 taatactttt ccggggccga cttcgatgac ttgttcgatg ccgattgttt ttaacttatg
    12901 gatactctca tagaaacgaa caggttgcat gacttgctgt tcaagtagtc ccgcgattgt
    12961 ttctttcttc attatttccg ttgttgtgtt gcttatgata ggaaaagaag gttcagagaa
    13021 ttgaatctga cttaggtcct tcgccagttt ttttgctgcc ggttctaaaa tagctgtatg
    13081 aaaaggcccg ctgacattta gagggatcat tcttttgaac cctttttctt ttaatagtaa
    13141 aacagcttcg tctactgctt tttcttcgcc accaatcacg atctgctgag gtgtattata
    13201 atttgcagga gatacgatgc cgtacttgct agcttcatga cagctttcct cgatcgtttc
    13261 aatcggcgca ttcatcactg caaccatttt cccgcttcca gctggggtag cttctgtcat
    13321 ataagcgcct ctttttgcta ctaacgcaac tgcttcagtg aaggatagcg cattactagc
    13381 tactaatgcc gaatattctc ctaaactcag tcctaaagct gcatctggga tgattccatg
    13441 ctccttcaat aagcgataat acgcgatgct gactgttaga atagctggct gtgtatattg
    13501 cgtctgatcc aaacgttcat tttctgtaaa gcaaagttca gccatatcgt agccgagaat
    13561 ctcacttgct tcatcaaatg tctgctttac aacggcttct tcatataaat cttttcccat
    13621 accttgatat tgtgcacctt ggccactaaa taagaatgct gttttcatgt ttttctccaa
    13681 tatatccaga atatatgttt gattcttcct attcaaaaag gccgagacat tcctatttca
    13741 ggaaatgtcc cagcccccaa cctaatttta atgattatgc agtttgtttt tctacgtaag
    13801 tcactaaatc ttgaaccgtt ttgattccgt cttctgtttc gattttcacg tcaaattcat
    13861 cttcaatttc attgatgatt tggaataaat ccaagctgtc agcttctaag tcttcttgga
    13921 tgtcagtagt caatttgatt tcttcagttt cttttcctaa ttcttctgcg atgatttctt
    13981 gaattttttc gaataccata ataatatatt cctccgatat tgtttgcttt ttatttttat
    14041 aatttaacag tctacaactc catgagaata cttccccatg ttaaacctcc gccatagcct
    14101 gtaaatatta ctttctgctg ttttcctaag gtgatctttc cactagaaac tgcttcatcc
    14161 aataagatag ggatgcttgc agcagatgta ttcccatatt tatccatatt ggtcaggaac
    14221 ttttcttgcg gcatctttac ttttcgggca attttctcaa taattctgag gtttgcctga
    14281 tgcaaaagca gataatcaac tggtttgtct ttcatgactt cttggatatt gtttgcaacg
    14341 tctcgtacgg caaaatcaaa aatatctctg ccagtcattt gcaaataata cgaaccttct
    14401 gaatcttctg aatagaacgg atttttattt tgctggtagc cagcggtcaa tgagctactt
    14461 ctggtaccgt cagctgctaa tttttctttc agcaattttt tctgtggatt cgcttctaag
    14521 agaatcccac ctgctccatc gccaaacaac acagcagtcg tacgatcaga ccaatcgatt
    14581 agcttagata gtgtttcacc accaatcacc agtcccttag ttgagccgca gcggatcatt
    14641 ttttcagcca tgcttaacgc ataaacaaat cctgagcacg cagcactgat atcaaaagcg
    14701 atagcatgag ttgctcccaa tttcccttgt acttgtgctg ctacagatgg tgtcgtgtaa
    14761 tcgggcgaca ttgttgctac taaaataaaa tccaaatctt tagggtttag atttttctgt
    14821 tttatcaatt tttccgccac taaaatgcat aaatcagaag tctcctgatt tgttacacat
    14881 cttcgttcac gaatacccgt ccttgacgaa atccattcat cagaggtatc catcatcttg
    14941 gaaagatcat tattagtaat aatcttttca ggaaggacgc tggctgttgc agtaatcatt
    15001 ccaaattgtt ccattaaata tcctcactta tagtcttgca agaagtcgtg caagtttttc
    15061 agtgcatcca gcaaggcatg ctgttcctcc gtagccattc cgtctaagat gttcttgacc
    15121 atttcacgat gaaaatgctg atggacccga aatagtaact ttcctttttt cgttagtccc
    15181 aactttacaa cgcggcggtc atcttcactg cggatacgtt cgacatagcc tttttttact
    15241 aacttgttaa tagcagtggt caaggtccca acagtaattg atagttcttt ggctacttct
    15301 gaagttgttt ttttcttgta catcccgatt gcctcgatcg tgtgcatctc tgtgattgac
    15361 agatctttga attgagattt tttcagctca gactcttcga ttgtcaaaat atcattgaag
    15421 acactaacaa gataatcatt tactgttttt aaatctggtt ccatgttcca ccgtccacta
    15481 attagtttga ttatcaaatc ttttgatgtt caaactatat cttaactttt ttcaaaatgc
    15541 aaccttaaaa gatcattttt ttactatatg agcttttttt aagattttac tcatttaaaa
    15601 caactgataa atcagcattt ataatttctt ttattagaaa aatcatctat ctcacatatt
    15661 ttgattatca aagtattttc tgtgaacaga actatagaat tccctaaaac ttgtccatta
    15721 tttcaaaatt tgttatagtc tttttattca aagcgtaaaa ggagatccta tgttaaaaga
    15781 aaaaatgaaa actctcccca tgctgccggg tgtctatctt atgaaagacc gagatggtaa
    15841 ggtcatctat gttggtaaag caaaacggct caaaaataga gtaagcagct attttcatca
    15901 gaataaacag cattcaaaaa aagtattacg tatgattcat catataacag attttgattt
    15961 cgtcgtagta gacacggaat tagatgcttt attgctcgag tgtcaattga tccaacatta
    16021 ccgtcctttt tataaccgac aaatgaatta tttcagtaat tataattatg tacatattac
    16081 caataaggga tttgtattaa cagatactcc tacggctcga acatacgggc cttttcgtct
    16141 ctataaaaaa atgccgtcta ttttgcgtat aatggaagaa acttatcaaa tgccttggtt
    16201 gtctgaaatt agtttgttag ctttgcgagt ccagcttcct gacctacagg aaatgacttt
    16261 tgaacagaaa aaaaaggaac tccaagggct gtttcaaggc cgaaataaaa agctactgac
    16321 ttacttgaaa aaaagacaac aacatttcat ctatcagttg aatttcgaaa aagccggtat
    16381 gcttcaaaaa gatatcgaat tagttactta ttttattaga cggatccagg aacaaaagca
    16441 gtttttaaga acaccatcgc tcaccttcag catgccatta gcagcagatg agagtcagaa
    16501 aaagcactac ctcgtttgtt atggtcaact tgcagaaaca atgattacct ctggtgacgt
    16561 ttctcctgac ttctattatg aaaagaaaga agctcattta tctttgaaac gtcaattgtc
    16621 aaaagaagaa atcgatcctg tacaaatctt gatcagttac cgcaaaaaat tagaaaaaga
    16681 acagtttgaa attgaactgt taaacaaaaa agaagctgaa aagcaactaa attagttgct
    16741 tctcggcttc ttttaggcat cttcaacttc aaatgtcaat tatcctaaaa tttattggat
    16801 taagtcatag atttccatcg ctacgatatc gatattatca aattcgtatt gctgatgagt
    16861 atctgtttca gttaattcaa atgtatcggt cgctttatca taagttactg tacacttctc
    16921 tacgccttct ttttcaaaac ggcgaacttg cagttcatta tctgctgact cgaccatagc
    16981 ctccagtcgt tttatgattg cgactaattg tgaatgtgtc atcggaggtc ctccttttct
    17041 tatttctgtt atattgtatc aaaagcatag cctacttgca ttgattttct ctaaaaaaac
    17101 tcgtttttta tgattttttt tctggtattt ttccttcagg ccaccaaagt ttgatcaaag
    17161 cttgggttcc gtcattctca aaacctttat ctgccaactc ttcatatagt ttcaatgctt
    17221 gggtagttgc tggcaataca agatccattt tttctgcttc ttctaatgca atctttaaat
    17281 ctttgataaa atgtttgaca aaaaaaccag gtgtgtaatc ttcttttaaa atacgaggag
    17341 aataattgct taaagaccag ttagctgcac ttccacctga aagcgtctca atcactttct
    17401 ttaaatcaag tccgtttttt tgtgcataga caagcatttc cgtcattcct gtcatcgtac
    17461 ctgcaatcat gatctgatta gccatttttg tatgctgacc tttcccagca gaaccatgca
    17521 atgtaaatgt cgtaccaaat tctttgaata aaggcagtac ttgatcataa acggcttctt
    17581 ctccaccgac catgatcgtc agtgtaccat tctttgctcc taagtcacca ccggataccg
    17641 gtgcatctaa cgcatctgcg cctttttctt ttgctgtttt tgcaattttt tctgccaggc
    17701 ttggtgtact tgtagtcaaa tcaactaaaa tatgtcctga gatatctgca gaaaaaatcc
    17761 ccgactgtcc atagtagatt tcttctacat ctttggggta acctaccatt gtaaagagaa
    17821 tattactttg ttctgctacc gcttttggtg tatctgccca gatagctcct tctgttacta
    17881 aaggatcagc ttttgctttt gtccgattat agacagctac ttcgtgtcct gcttctagta
    17941 gatggcgtgc gacagcactt cccatgacgc ctgttccgat aaaaccaagt ttcatttttt
    18001 cgacctccta aaaatcaaaa atccttgctt ctatgctagt atacgctaga aacaaggatt
    18061 ttttcataaa aaatgtttga attttatctt ttaaatgaat ttaattttat tttgattcgt
    18121 tcaatcgagt cttcttcttc ttttcttcgc atctcttctt catgataaac aaccatgtct
    18181 tgataaaatt cgatcatagc ttcgccgaaa tgtgtagatg aaatatcata aagtttagct
    18241 tctaaaactt ttggatcttt aggaacttca ctggaaatat aatcaataag tgctggtgca
    18301 aagtcttcgt cggtttcaaa agttttccct aatgaaggat gatcgaacaa tcggttcaaa
    18361 taagcatttc cttccacgac acaaggtgtt ccggctgcca tcgcttctgt ataagtaagc
    18421 ccttgtgttt ctgaagtaga agcactgaca aaataatctg ccgctttata ataaagtgct
    18481 acatcattgt tgtccacttc tcctgtgaac tgcacaaatt cttctaactg cagttctgag
    18541 atcaattcct ctaactcttc tacataaggt ccttttccta cgataactaa tctggcgtta
    18601 ggataagcgg agatgacctg aggaaaccca ttaatcaagg cttggatatt tttctcataa
    18661 gaaatcctgc ttaaagaaag aatcatcaaa tggttttcct gaagacctag tgactccctt
    18721 aatttgctga tatcctgctg agtgatatca gaacgtttga atttttcaat gtcgatacca
    18781 gtaggaatca cgcgaagcgg tgctattaca ccatagctgc gtaatgtatc aataacacgt
    18841 tcacttggac aaactacacc tgtcgtatga tttgcaaata atctagacaa gtattttaca
    18901 tgggattgcc tgatcacttt tcctttaccg atataatgaa ggtagtcttc atacatcgta
    18961 tggtaagtat ggatcagagg tattttcaac ttcttagcta ccatcttccc aagtatccct
    19021 gctccgaatt ctgtatgggt atgaataaga tctagttcta gttctttggc aaccaaataa
    19081 gcataccaca tgcctctaac aacaactctt cgatccttga aagaaataaa tggtacactt
    19141 ggcattcgaa tcacatcttt ttccaactta tccgcattag gatcggtcgt tgtaaaaata
    19201 tagacttcat gcccatgtct ttctaattct tgttttaatg ttttgatcga tgtagcaact
    19261 ccgctgactt gcggaaagta ggtatcggta aaaaagccga ttttcatgtt tttcgcctcc
    19321 aaactagtcc ttgacctgat ttactttttt taatttgcta gagacttttt cattcagtac
    19381 actttcgtat acatttttaa gttcttcacc gattttattg atacttcgct cttttgctgt
    19441 ttgataaccg gcttgtgtcg tactcggtag ttcttgattg gtaatctgtt ggatcagtcg
    19501 gataaattct tcgtttgtct tccccatata gcagttcttg ctatcttcta accagccatt
    19561 atacactgga atatctcgaa caagtacgtt ttgtttgctt gctaaagctt ctaaaacaac
    19621 gatcccttct gtctcttcat aagaagggaa aaagaataga tctgcacctg aatacgcacc
    19681 ttctatgatc tctccacgaa tgtaaccagg aaacaggaca ttttcaggat gatcttcttt
    19741 tacaattttc cggatgttcc gaggaatcga gtacatggga acatgaccaa accaaataaa
    19801 tgtgtattcg ggcatcttct tagcaatttc tacgaaatca atgattcctt tacgttcgaa
    19861 aaacaaaccg acacaaatga ttactttttg atccggagac agtttgaaat attctctgaa
    19921 cttttgttct ttcattggat cagggtgata tttctctaag tcgatcccat tggaaatagc
    19981 ttgaatcggc acacggatac catagctttc caacaactgt tttgaataag gtgttggcgt
    20041 gatcagatag tccgcttttt ggtacaatcc taccagatat ttttttacga taggagaaag
    20101 ctggttcgat ccgataaagg aatttcggaa atcttcctct gtagaatgcg catgatagac
    20161 aacttttttt ccttctcttc tggctttatt gaccatatta tgactattga ttccgtatgt
    20221 attgatgtgc aggatgtcat aatcttcttt ttcatctagt gtataagaga taccgacttc
    20281 cgtcagagca cgtttttgat gatcaagcgc acgcccgatt ccagattttg caagcatctt
    20341 ttcactttca aaatataata ataccttcac cttacgtccc ccttcactta tatagtataa
    20401 catagcctac aaactgtcac tcaaacttaa gccctatttt tgtctattct tactgacaaa
    20461 aacgttttct taaaaaagaa caaaaaaact ggcgtagctc ttttacaagc cacaccagct
    20521 tttgatacgt tgtttatttt acatattcat gaacaagttc aacgacttct tccatcatat
    20581 cgcattcttt caatgcacga tcagcaagtt cagccatttt cgctgtgtcc aagcgtttca
    20641 ttaaactacg cgttttaaga atagatgtcg cactcattga gaactcatct agacccatac
    20701 ctactagcaa tggaacagct gtttgatctc cagccatttc tccacacata cctgcccatt
    20761 ttccttcagc gtgtgctgca tcaattacat ttttgatcaa acgtaaaatt gatgggttat
    20821 atggttggta caagtaagaa acgcgttcgt tcatacggtc tgctgccatt gtgtattgaa
    20881 tcaaatcgtt cgtacctaca gagaagaaat ctacttcttt tgcaaacttg tctgctaaga
    20941 ctgctgctgc tggaatctcg atcatgatgc cgacttggat cgtatcagat acttccttgc
    21001 cttcacttat caatttttgt ttttcttctt caaagatcgc ttttgctgcg cggaattctt
    21061 tgagtgtagc taccataggg aacatgatac gtaaattacc atgaacagat gcacgaagca
    21121 atgcacgcat ttgtgtacgg aacatttcgt cgccttgttc agataaactg atacgcaatg
    21181 cacgatatcc taagaatgga ttcatttcat gaggtaattg cagatatggt agttctttat
    21241 ctccaccgat gtccattgta cgaacaacta caggttttcc ttccatacct tctaatacag
    21301 ctttgtaagc ttcatattgg tcatcttctg ttgggaagtc tggagaatcc atatacagga
    21361 attcagtacg gtacagacca actgcttctc caccattatt atggacacca actagatctt
    21421 ttggtgttcc gatgtttgca gctaattcaa aatgcttgcc gtcagctgtg acagtttctg
    21481 cattttttaa tttttcccat tctgcttttt gtgctgcgta atctgcacct gctttttcaa
    21541 attcagcttt ttgttcatct gtaggatcga tgattacatc gccttcgatc ccgttgacag
    21601 ccaaaatatc gccttctttg actttagcag taatttcttt tgtcccaacg attgctggaa
    21661 tttctaaaga acgtgccatg atagcagaat gtgaagtacg tccaccaatg tctgttacaa
    21721 acgcttttac aaaattgcga tctaattgtg ctgtatcact tggagtcaag tcatgtgcaa
    21781 caacgaccac ttcttcgttg atcattgatg gatttggtaa agtaacgcct aataagtgcg
    21841 ctaaaatacg tttagcaacg tcacgaatat ctgcagcacg ctcttgcata taagcattat
    21901 cttccattgc ttcgaacata ccgatataca tatccgttac ttcttttagc gctgattcag
    21961 catttacgct gttgtcttta atattttgct tgatttgacc taccatttct ggatctgaaa
    22021 gaaccatcaa atgggcatca aatacttgcg cttctgcttc acctaagctt tgcgctgctt
    22081 tttcacgaat ttgctgcaat tcttcagtag atttagctaa tgcagcatcc aaacgagctt
    22141 cttcagccgc agtatcttct actgttgcct tactgaatgt taaatccggt tgaactagca
    22201 ggtaagcttt tgcaacggct acaccgtcac tagcggcaat cccttttagc atttcaacca
    22261 ttattcagat aagccttctt tcttcattgt ttcaacgatt gctgccattg catcagcttc
    22321 gtcagcacct tctgcagtaa tagtaacatc agaaccttga cctacaccta aagacataac
    22381 gcccatgatt gatttcaagt ttactgattt acctttatac tctaagttta catcagagtt
    22441 aaatttgctt gctgtttgta ctaatagtgt agctggacga gcgtggatcc cagtttctgc
    22501 tactacgtga aattctttct tttccatatt aatcggtctc ctttaagtga gtgttgtttt
    22561 tatgtgaggg ttttcttttt tagtaattca gagaatccta acaaatgcca ccctttcatc
    22621 tgtaaagatt aacatttttt ttcgttagat acaacagttt tcattcaatt tcagataaaa
    22681 gtattcaaat tattccggat tctttttcat ccttttcttc acattttata acaaacgtgt
    22741 ttatttcctt tttcttgtcg ctttctcttc tcctgacgtt tatcattctt tcctttgacc
    22801 ctctttgttt tcatatgaaa cgaattactt gcttcccttt ttatttttag tataataaga
    22861 gtcaaaggtc aggaatggtc aatagagatt tcaccttttc tacctcataa atctcttaaa
    22921 aaactagggg ttattctttt acctcatgaa aaacgagatg taaaatatgg atgtacccat
    22981 ttttgtgaaa ggacgtgaat gattcatgct ttgtcaaaac tgcggcaaga atgaggcaac
    23041 gattcacttg tatgccactg tcaatggtca gcggacacaa ctcgattact gtcagagctg
    23101 ttatcaaaaa atcaaaaatc aacaaaatgg aggtctgact aatatggccc aaaatgatcc
    23161 atttggattt ggcagtttgg acgatctttt ccgttccatg tctcgccaaa tgcaacagca
    23221 aggaaattat gagcaaacac caccaacaca atttggcgga aacaacaact tcaatggtgg
    23281 tcagccacct caaggcaata cagaaggttt attgggagaa tacggaatca atatcacaga
    23341 acaagcacga caaggtgata tcgatcctgt gatcggacgc gatgacgaaa tcaaacgagt
    23401 cattgaaatt ttaaatcgcc gaaccaagaa caatcctgtc ttgattggag aaccaggtgt
    23461 tggtaaaaca gcagttgttg aaggtttagc acaaaaaatc gtagatggtg atgtaccgca
    23521 aaaattaatg gacaaagaag tcattcgttt agatgtcgta tctttagtcc aaggtactgg
    23581 tatacgagga caatttgaag aacgtatgca aaaattgatt gaagagatcc gtcaagcaga
    23641 aaatgtgatc ctattcatag atgaagttca cgaaatcgtc ggtgctggtt ctgctggtga
    23701 tggaaatatg gatgctggta atattttaaa accagcttta gctcgaggag aattgcaaat
    23761 ggttggtgct actaccttaa atgagtaccg catcatcgaa aaagacgctg ctttagaacg
    23821 tcgtatgcaa cctgtacggg tagatgaacc aactgtcgaa gaaacgatca gtattttgaa
    23881 aggtttgcaa aaacgttatg aagactatca tcatgtaaaa tatactgatg aggccatcga
    23941 agcagccgca actttatcca atcgttacgt ccaagatcgc ttcttgccgg ataaagccat
    24001 cgatttgcta gatgaaacag gttctaagaa aaacttgacg atccaaatcg ttgatcctaa
    24061 aacaatcgag aaaaaactgc aagaagcaga agaacaaaaa gttttagctt ctcgagaaga
    24121 agatttcgaa aaagcagctt attatcgcga tcagatcaat aaacttcaaa aaatgaaaga
    24181 acgtcagctc actgaagagg aaacacctgt gattaccgaa aaagatatgg aaaaaatcgt
    24241 tgaacaacgg acaggtattc cagtgggtga attaaaagaa aaagaacaga cacaactgaa
    24301 aaacttagca gatgacttga aagctcatgt catcggacaa gataacgctg tagatcgagt
    24361 agcgaaagcg attcgccgaa accgtgttgg attaaacaaa cagaatcgtc caatcggctc
    24421 cttcttattt gttggaccaa ctggtgtagg taaaacagag cttgcgaaac aacttgccta
    24481 cgaactattc ggttcacaag attcaatgat tcgtttcgat atgtctgaat atatggaaaa
    24541 acatagtgtc tctaaattga ttggttctcc tccaggttat gtcggatacg aagaagctgg
    24601 tcaattaaca gaaaaagtac ggagaaatcc ttacagtctt gtcttgttag atgaagtaga
    24661 aaaagctcat ccagatgttt tgcatatgtt cttgcaaatc ttagatgatg gtcgattgac
    24721 agatgcacaa ggaagaacgg tcagctttaa agatacgatc atcattatga caagtaatgc
    24781 cggaactggt aaagtagaag caaacgttgg ttttggtgcg gcacgcgaag gtgttactcg
    24841 ttctgtattg aaccaattaa acaactactt tacaccagaa tttctgaacc gttttgacgg
    24901 gatcatcgaa ttcagtgcat tatcaaaaga aaacttgatg acaatcgtca cactaatgct
    24961 agacgatgtc aaccaaatgc tggcagcaca gcaacttcac atcgaagttc ctacgaatgt
    25021 caaagaaaaa ttagttgact taggctatga tccatctatg ggtgctcgtc ctcttcgacg
    25081 cacgatccaa gaacaaatcg aagatggtat cgctgaattt tatcttgatc acccttctat
    25141 ccatgagttg aaagcaaaac tggataaaga tggaaaaatc atcgttactt ctaaaccaga
    25201 acgtttggct aaagaatcag ccgaagaaac ggctgaataa atccagtttt atagcgtgag
    25261 acggtatttc tttgagaaat gccgtctcat tcttttttca gaaaagtcaa aatactgttc
    25321 attttataaa aaagaaatta taataagaga aacaagcaat taaaaataca taaaagaggt
    25381 gagcgccatg aaattggtaa atgtaacaaa cagccattcc cgtcttgtat taaaccagct
    25441 tgaaaacact gatgcccatc tagtcaaggt ctacacagcc ggaaatacca ctattgtcta
    25501 cactgaagcc cctgaacata atgaaattct gctgatcaat gacaaacgta aaatccagcc
    25561 gaaagaaatc gaagatgcta aaaattattt cttagataaa atcaaagacg ccgtttacca
    25621 cgaggatgaa atcaaagtaa tcgaattggc aggccttgtt gaaatctcga ttccaaaaca
    25681 tgaattaggc gctacattag ccgtgtaaac aaattttgaa cataataatt aaagagcgtg
    25741 ggacaaaagt aaagaacact tttgtctcac gctctaaaca cgtataaacg gcgaaagcag
    25801 aagcaactcc ttcggaaata agctgaaatt cacgaaaatt gcttttcaaa tttttgtgaa
    25861 ttccagctta tttcttggag ttaaacgctt ctgtctcagc ctctttttct ttctagcttt
    25921 ttgatttttc tcttttcttt tgtacttttc tcttttcatt ttcagttcta cgcgtgtctt
    25981 tatttttaac tttttcctct ttatggatga ttgatcgtac tttttgtgca atttgctcgt
    26041 ttttgtccca gtcttttaca tctaataatc gacataatcg taacatctct tcttttttct
    26101 gtttttttct ttcacgtctt tgccattctg tttccatgtg tcacactctc ctttctttaa
    26161 tcatatgtac gcaaaataat ttttatattt ttttgattag acatttctat gattttaata
    26221 tagtaaaaag agccgtcacg gaaaatccgt cacggctcta aacaaaatat gagaggctgt
    26281 ttttgtttgg ctattcttat tacttgatgc agggcagctt gtgtcaagac ttctttacat
    26341 caaacttttt aattttacat tgggatattt atccgcaaac cagcgttctg caaattggtt
    26401 ctcgaataag aacaacggtt ggtcgaaacg gtcacgagcc aagatattgc gacttgaact
    26461 catcttctca tccaaatctt ctggatcgat ccagcgagcg attttgtgtc ccattggaga
    26521 catgattact tcggcgttat actcatttaa catacgatat tggaatactt cgaattgtag
    26581 ctgaccaaca gctccaatga tatattcttc cgttagatat gttttataaa gctgaatcgc
    26641 tccttcttga actaattgat agattccttt gtggaaggat ttttgtttca tgacattttt
    26701 tgcagtcact ttcataaaca gctctggagt aaatgaaggg agttcttcat aagcaacttt
    26761 tagttttcct tcgaacaatg tatccccaat ctgataattg cctgtatcat atacaccgat
    26821 gatatcgcct gctactgctt tttcgacgtt ttctctcgaa tctgccataa attgtgtgac
    26881 attgcttaat ttcagttttt tatttgtacg ttccaaccag acatccattc ctcgttcgaa
    26941 tgttccagaa cagattcgga caaaagcgat tcggtcacga tgggctggat tcatatttgc
    27001 ttggatcttg aatacgaaac ctgaaaattc ttcctcataa ggacttacca tctgatcatc
    27061 ttgtgttttg tgtccataag gagctggtgc caattcaacg aaagtctcta aaaaagtctg
    27121 tacaccgaag ttagtcaatg cagaaccaaa aaatactggt gtctgatcgc cacgagcaat
    27181 tttttcttga ttaaatgcat ctccagcctc tttgattagt tctacttctt ctaagacttg
    27241 ttcatataag gaattttcag ttagaggatg attttcggga agcagtccat cttcatccaa
    27301 ttctactaat cgttcatcct gataattttc cggacgataa aactctacac gattatggta
    27361 gatatcataa agaccttcta gccctttacc cataccgatt ggccaattca tcggataaga
    27421 ttcgatatct aatagctctt ctaattcttc taacaattcc aatggttcac gaccatcacg
    27481 atctagtttg ttgataaaag taaatatcgg gatgccacgt ttttttacaa cttggaataa
    27541 tttctttgtc tgtgcttcga tcccttttgc actatcgatt accatgaccg cactatccac
    27601 ggccatcaaa gtacgataag tatcttcaga aaaatcttca tgtcctggtg tatccaagat
    27661 atttactcgt ttcccttgat aatcaaactg cataacagaa ctagtaacag aaatcccacg
    27721 ttgtttctcg atttccatcc agtctgattt ggcaaaatta cctgtttttt tgccttttac
    27781 ggtcccggct tgacggatag ccccaccaaa caatagtaat tgttctgtaa tcgttgtttt
    27841 cccagcatcc ggatgggaaa tgatcgcaaa agttctgcga ttgtccactt gttgctttaa
    27901 ttttggatta ttcataacct aactccattt cttccattca attatttata ttttctctag
    27961 gcacagattg tccgatctct taaagaaaga caatttcata tagggctgtg acaaaagccg
    28021 tgtagcagtc tctttttccg agtaaacggc gttctagaga cagcctatca gcaataagcc
    28081 cacccaagcc aaaaatacga aaagctgttt ttgcttggct gctcttatta cttaatgcag
    28141 gatagctttt tcacaacctt tgtcaactac tacaactgaa tcagtatagc atggattcag
    28201 cgaatgaaaa agccaccaaa ccataagagt caaatatttt tttgtcagct gactaaaaga
    28261 aaagacgtgt tgcttgttta ttcaagcaac acgtctttca taaccaagtt tccaattatc
    28321 ttcttggctc gtcatcttcg aattcttttc tgaagtatct tcgttcttct tcaggttccg
    28381 cttctgtttc ttccggatca tgaaaaatga cacggatttt aaggattcga gaaccttcca
    28441 tttcttcaga aaccagcgta atattatcga catcaaacga taacttttcg ccttcatccg
    28501 gaatcatgcc taatgctgta atcaaataac cagccatcgt atctacatca ctcatatgca
    28561 gatcactgtc aaaagcttca ttgaactcat caatcagcat acgtccttgg atgatatatt
    28621 cgtgttcatc gattttttca taaagattct caacttcatc tgattcatca tcgatttctc
    28681 cgacgatttc ttctaaaagg tcttctagtg ttgctaaccc aacgacgccg ccatattcat
    28741 ctaataaaat agccatctga ttctgcgtct ttttcatttc atataacaga tcatcaataa
    28801 agaccgtttc aggaacgaat aaaggctcct gcatgatttt cttgatatct aaattatcaa
    28861 agccgaattt atgggcagct tttagtaaat tctttgtatg aaggattccg actactttat
    28921 ctttgtcttc gttataaaca gggattcttg aatagttttc ggataaaact tcattcacat
    28981 tttcttccac agtgtcgttg atatcaatca taaatgcatc cgttcgtggg accatgactt
    29041 ctcgggcaac tttcgtatca agagaaaaaa ccccttggag catttctaat tcttcattat
    29101 tcaatacgcc ttcgttttca agcatataac gcatttcatc tctggtcatt tttgaatctt
    29161 catcatcaaa agtcatcggc gtgatacgcg ccaataaact tgtggaagca gaaagcagcc
    29221 agacgaaggg cttagctact acaccaatca agcgtatcag ccctgaagtg aactttgcga
    29281 cttcttctga tttatttaaa gcaatacgtt tagggtacaa ttctccaaaa acaatcgata
    29341 cataggtcaa aatagccagc acaatgatat ttgcaatatt ttttgccgct gtgccgccac
    29401 ctaataccgg agctaaccgt tccgataaag tattagctag tgaagcacca gacaaaatgt
    29461 tgaccaaagt gattcctact tggattgtcg ataggaaatt atttggattc tgcaatattt
    29521 ttaaaagctt ttgagatttc acatctcctt catcagcttt ttgctcgatt cgattcttat
    29581 tgatcgaaac aacagcaatt tcggaagctg ctaaaaaagc attgatcaat gtcaaaacga
    29641 tcaataataa aatctgcgct aacagcgact gactctcagg gtcagcattc ataatcgcat
    29701 tctctcctta taaaattaat ttttagttta cgatagaaaa aaacgtggca ctatagccac
    29761 gttaaaatgg tatcacaaag caattttctt tgcaataaaa acaagtgtac taaaactacc
    29821 tacgaggtcg tagcaatttt cttcttgttt tcattttgct tgtcttcttt gacgattttg
    29881 attatatagg aaatgatcgt cttgcagatt gcgtaaaaag gaacacctaa gaagatacca
    29941 agcaatccgg caatatttcc agctactaga agaacgataa tgatcgttaa tggatggatt
    30001 ttcagtgatt tcccaattac attaggataa atgatattcc catccaactg ttggacgatt
    30061 agcacaacca cacaacaaag taatgctttt accggatcat tgaagactgt tactagaaat
    30121 gcaggtgcta gtcctagata aggtcctaaa tatggaatta ggtttgtcaa tcccgcaatg
    30181 accccaaata agaaagcata atccacacca attaccatat atcctaaaaa tgtgaaggta
    30241 ccaacgaata aacattcgat tgcttgtccg ctaatgtaat tagccaatgt tttattcagt
    30301 tgccccaata actccacaat ttggtttctt cttttttctg ggaaaaactg ttggatgttt
    30361 ggcactagtt tttctccgtc tttcaacata tagaacaaga tgaatggtgc tgtaatgatt
    30421 actacggttg ccgaagcgat cgttccaacg atcgaaccta aactattgga tactccacta
    30481 aggaattgct gaatgattgt cccgtaagaa atgtctaatt gctcaaaata tttattgata
    30541 tctacttcct tgaacaaagg caattcggct acctctttta accagctttc tacctgtttg
    30601 atgaaatcag gcatattaga tgccaatgag gaaatttgct gtactagact tgggatgaca
    30661 ctaagtaaaa tccaaataat tgctgcaact aaaaggagca gcacaattgc gacagcccat
    30721 attcttttga tttttgcttt catcaacagc ttgacaatag gattcaataa atagtaaaga
    30781 aagcctgcaa tcagtacagg tgcaaataac gtggaaaaaa atgtcccgat cggttcgaaa
    30841 ataaagttta tttctgatga aatcatgatc aatgcagcta tgatcaataa ttcaagcgac
    30901 caaaacatta gtttagactg tctaagtttt tcaaacaata gatttcctcc tttctttctt
    30961 actagtatat attatattcg ttaaaaattc ttttattctt tttttatttt tgaaaacttg
    31021 ccatactttt tatttgtttt attttccagt atactatacg aaaggaaggt ggctagacca
    31081 tgaaagttgt acaaacaaaa gatacaatga gcaatattta cttagatgct gtacggatca
    31141 ggcatcaagt ttttgtagtc gaacagggtg tgcctttgtc tcgagaaata gacaaagatg
    31201 aagcccattg tatccacttc gtcctgtact cagataaaaa agaaccgcaa ggaactgtac
    31261 gtcttttgcc tttagaaaac ggaaaaatga aattacagcg gatggcgatt ctttctgaat
    31321 acagacacca agggttagga aaaatattga ttgaagaagc agaaaacttt gctaagaatc
    31381 aaggctataa tacgatttta ttaggggcac agtccacagc tgaaactttt tacgaaaaac
    31441 taggatatac agcttacggc gatccttttg aagatgctgg catgcctcat atttatatga
    31501 aaaagacttt ataaaaattt atttcgaaaa aagcaaaaaa cagcaagctt ttgagaaaaa
    31561 tctctcactg gcttactgtt cttttttgtg tttacttacg cgacaattgc ttgtaacggt
    31621 tataccaaat atcaatatag ccttccgaga acgggccatc tccattattg atccagtcaa
    31681 ccagaatctt cacattttct tttaggatga agtcaatatc gttcgggtaa ttcatcattt
    31741 tgctgtgcat ctcatattca tcgacatcca atagtcgttt ctcgccgtca ggaaatacct
    31801 tgatatccaa gtcatagtcg atatatttca aggcctcatc gtccagcaaa aaaggtgaag
    31861 ctagattaca ataataggaa accccctttt ctctgatcat tgctattaca ttgaaccagt
    31921 attttttatg aaaataaaca atcgctggtt cgcgcgttac ccatcggcgt ccatcagatt
    31981 cagtgactaa tgtgtgatca ttcacgccga tcatagagtg ttcgcttgtt tttaatacca
    32041 tggtatctcg ccacgttcga tgcaatttgc cgtcgtgctt gtaactttgg atcgtcacaa
    32101 actctccttc ttttggaact cccatagcaa cccttcctta agcagcattt tgtgctttgt
    32161 gtttgggtgt tcattacacc gacatcataa ttattatatc aataaaaatg agctttgcct
    32221 agaaaggatt cctattgttt cgatttttct ctaactgatc taccagtttc atttggacct
    32281 taggaaaaac gaccgaatca aatgcatcag gcttcaacca cttcgaagtt ttgttctcgg
    32341 taaattcttg ttctgctccc tcggttgccc gtccgtaaaa aaggagaaca tgccatttca
    32401 gatgactaaa aatatgagta acttctccta aatgtcgtgt ctgccaaaca aagagttcct
    32461 gcttttcaaa tgggagattt tgctcttctt ctgctactag gtcatcaaac agatcgattt
    32521 ctggctttgt ttcccattct tttttcaatc gctcatactc ctcttgtgtc acttccatca
    32581 ttggaaatgt ccacatattc gccagcaatt tttggctatc tctttcttca aaataatacg
    32641 ctccagaatg attttgaaga gctgcactga tataataaac gtcttttggc tttgcttttt
    32701 ttgtttttac aggaaaactc gtttggattc ctcgtttgtt agctaaacaa aaggcttgga
    32761 tcggacaagt ctcacacttt ggagaagttg gtgtacagat agctgaacct agatccatca
    32821 ttgcttggtt gaattcacct ggatgctttt catcaatgat tttccgcatc gcttcatcaa
    32881 agattttcct gctagaagct tttgcgatgt ctgcttcaat gcaaaacaat ctgctgacta
    32941 ctcgcatcac gttgccgtca actgcaggtt ctggaagtcc aaatgcgata cttgcaatcg
    33001 cgcctgtcgt atatggtcct attcccttca atgagctaat ttcttcaggc gtttgcggca
    33061 tttccccatc aaattctgac atgatctgtt tagcagcagc ttggatgttc cgggctcttg
    33121 agtaataacc aaggccctcc catgctttta aaagcttttc ttctggagca tttgctagct
    33181 cttcaattgt aggaaaccat tccatgaaac gataaaaata atcgatcact gtgtctactc
    33241 tggtttgctg aagcatgatc tcagagatcc agatacgata aggatcacga ttatagcgcc
    33301 aaggcaggtt tcttttttct tgttcatacc attgaatgaa ctgatcttga aattcttttg
    33361 tttctttatc tgtccatttt tcccattctt tattcacttt cctcttcctt ttccgtaatg
    33421 aatcgctgga tcatatccaa atcaaatcct ttttgatata agctttgctt tacttttaga
    33481 tttctttttt taggttcaaa ccttaaattt cttcgccata gtttatctcc ttgtttgact
    33541 aaataatcat attctatttc ttgatctgct tcttgcggaa gatcagctat cgcttgttgg
    33601 atcacttctt gagaaaagcc tttcgtcatc aatgtttgtt ggatttttct caataattct
    33661 ttttggcttt ttccgtgatt ttttccaaac gctttttcgg ctgtctgatg tgcattttga
    33721 acttggtctt caaatgtata ttggctgagc gcttgttcaa tgatttcagg tttcaaacct
    33781 ttttgctgca tctgctgcgc taatttcttt ggtcctttgt cacttaagcg catattcgtg
    33841 cgcacataac tttccccata gaccaaatca tcgacaaggt ccaattcttt taagcgggct
    33901 acgattttat ttcgatcttc ttgaaggatt tccttttctt ttaagtatgt tcggatttct
    33961 ttttccgatc gcaactgata acttaagtaa ttcattgcta ggtgaaaacc gaaatcagct
    34021 ccgctactct tcttgatctc ttggatagtt tcttcgtcta attcccgtcc ttttaataat
    34081 cgatagcgta ctaatacatc ttcagatact tgtaaagctt ctccttcaga taaatctacg
    34141 cgatagaaag ggcctttgcc tttactgatt ctcgtaacgg ttaacataaa catcctcctc
    34201 gaacatatat tctctatcat accacagaaa atccccgtaa tcaggtatta tatgttagaa
    34261 taaaccacag aaaaaagaaa aagggagata cttatggcag aaacaattgt caaaactgga
    34321 caaacggttc ctttgaaaat caagcgttta ggcataaatg gtgaaggaat cggttatttc
    34381 aaacggttga tcatatttgt tccatatgca ctaccaaaag aagaagtact agtgaaaatc
    34441 acgaaagcaa ctcctcgcta tgcagaagga gaattaataa aagtcaaaaa aacaagcaaa
    34501 gatcgcgtag tggcaccatg tcctgtttac tatgaatgcg gtggctgtca attacaacac
    34561 ttagcttatc aagctcaatt ggattttaaa aaggatctgc ttttgcaagc attggaaaaa
    34621 ttcaaaccag caggctttcg atcttatcaa cttttgccga cgatcgggat ggatgagcct
    34681 tggcattata gaaacaaagc acaatttcag ttacgaaaaa ataagcagac tcaaaaaatt
    34741 gaagctggtt tatacgaagc aaactctcat cagttagtcc ctataacaga ctgcttggtt
    34801 caagaacctc gaacacaaaa agtaatgaat actgttgttc aactattgaa taaatttgat
    34861 gcacccatct atgatgaacg gacaaacagt ggaatcttcc gcacaatcat ggttagaatc
    34921 ggtgttaaaa ctggggagtt acaagttgtt tttattactc gcagtaaaaa attcccgcaa
    34981 aaaaacgcaa tgatccgtga gatcaatgaa cgactgcccg aagttgtttc aatcatgcaa
    35041 aatgtccagg cacaaaaaac gtctctcgtg atgggggatg aaacgatcca cttatgggga
    35101 aaagaagcca ttgaagaaca aatcaatgaa gtgactttcg atctttctcc tcgtgccttc
    35161 tttcaactca atcctcagca gaccgaattg ctttatggcg aaggaatcaa agcattagat
    35221 gtccagccta atgaaacgat cgtggatgct tattgcggag ttgggacgat cggcttaagt
    35281 gtagcaaaac aagccaaaga agtacgggga atggataccg ttcctcaagc aatcgctgat
    35341 gcaaaaaaaa atgcggaaag attaggatat cacaacactc gatatgaagt cgggacagca
    35401 gagcaagttc tgcctaaatg gttgaatgaa ggttttcagc ctgatggtat catcgtagat
    35461 ccaccacgta cgggtttgga taacgcattg atccaatcat tattgaaata ccctccaaaa
    35521 aaacttgttt atatctcctg taatgtttct acacttgcca gagatctggt taatttaagt
    35581 aaaaagttca agattgaata tttacagtcc gtcgacatgt ttcctcagac tgcacgttgt
    35641 gaagttgtcg tgaagatgac gagactcgct gactaaatga aagaggttgt gaaaaagtct
    35701 gccctgtatc aagtaataag aacagccaaa caaaaatagc tcttcatgtt ttatgaatac
    35761 tgtacttgtg accgaagagt taacaggagg acactattta tttaaaaaaa gggttgtgac
    35821 atagtttttg tcacagccct ttttgattat tattggatat tcacgtctat cacttgataa
    35881 aatgcatttg tcgtatcgct gatattccat actgctaaaa tcacattata tccttttcga
    35941 tcctgcggaa gtgtgatcgt ttggttgact gtttttggtg gaacagttgc tttgtcgtct
    36001 atttttgtga tcaattcaaa atcactaaat ttcaaaggtt gattaggatt ccaagttggt
    36061 ttggtaatgt agtaatccca agtacttgtc cgatgttgtg ctgttaaatt ccaagtaata
    36121 tttaatgttc ctggattaac catgttttta tgccatctgt tagatgtctg ttcatctaaa
    36181 ggctcaaagc cgctgactcc tgcactagca atttttccat caatgaatgt atttttggta
    36241 gcttcgatac tttgcggttc ccattgtgct cttccgacat tttgatttaa gtttcctgct
    36301 gctgatgttc ctaggtatgc acggcttcct ggagaagtaa tataaccatg cgcaaaacct
    36361 ggaatgctaa aaccgaacaa ccctaaacta actaacccaa taaacaataa cgtactaatc
    36421 tttttcatgt agaaacctcc ttcatttgat aaccacattg taaacgcttt attgtattaa
    36481 cacaaacatt atgcttttat aattttatgt tgaagtaaaa aaggtgaaaa aagtaagatg
    36541 cagattataa cagaagtaga cgagaaaagt acaaatagaa aacatgagaa tcaaatcata
    36601 tgatcttttt caaaaacatc attcatataa acaaatgttc aactttttaa taacaacaat
    36661 tatcaatttt tttcttactt cacaaactaa atacaggtct tgctgtgagc cttcatggta
    36721 aactaatatt atagaaagga gttagtaaaa tgaagatgat aaaagatatt cgtttttggg
    36781 tttgtgttat aggaattgtc atattaggct ttttatcagg gttgcttagc ggaaatcccg
    36841 gaaaatatta ctattctttg caacttccac catttgcacc acctagctgg atatttgggc
    36901 cgatgtggac cttactgtat atattgatgg gtatttctct ttatttgtta cttaatcaca
    36961 acaacaaaaa acagcgaaac aatcttgtcg gtctttttgt gattcagttc atttttaatt
    37021 ttatatggtc tgctctattt tttaatttaa gaaatatttt cattgcagca atcgatatta
    37081 cacttttagt tattttcttg tcagtgctta tgtatcagtt gtggctgcat catcgcctgg
    37141 caatgtggct catgattcct tattatttat gggtactatt cgcaactcta ttgaattatt
    37201 caatttattt tttaaactaa gcgctaaaaa gagacgtcct gtaatttaac acatagaaaa
    37261 agtgccacct aagttagatt ttttgtctaa tttaggtggc actctttatt aaccaaacta
    37321 tttttttcta gtacccactt gttgttccat actcattatt ctggtcataa ccataaccat
    37381 tttgttcctg tccagcgtaa ggatcataac tttgagtatc ttgacttggt gtatagtact
    37441 gacttgtatc ttctgtttgc tgataatagg tattcggatc ttggtactga tcatatgtgt
    37501 tttctgaata ggaagaataa tcggtgtacc cttcactatt cgatccatca ccgtttgtcg
    37561 aatcgccatc ttccgaatca tcaaagaaca tcattgaagc attctcattt ttcaaattag
    37621 gtaatgtatc gcttgttttt atatttaatt gtttcttcag ttcattttgg atcgaaagaa
    37681 gatcattttt tcctaaaatt tgatagtaga tatcattgat catttgtcct tcgccttgca
    37741 actggtcttg tttgattgtt tcaaacgccg gggtgtagtt cgtcgctaca tcaagcatgt
    37801 catcccaatc taagtccgtc gtcacattgt tctcaactgc ttttaaaatt tttctataat
    37861 tgcttacgcc atctaaactt aaaactttat tgacgatttt cgtcactact tcccgctgac
    37921 gacgttgtcg accgatatcg ccttctggat cttcgtgtcg cattcgagaa taggctaaac
    37981 ctttttctcc attcaatgtt tgttcaccta ctggtacatg gacaccatcc aaagtaaagt
    38041 caattttatt attcactttg atgccaccga ccgcattgat caaatcttcc atgccatcca
    38101 aattgatcgt tacataatga tcaataggaa tatcgagcag ttgctcaatt gaatccatgg
    38161 ccatttcgac accaccaaat gcgtaggcat gatttaattt atctaccgtt ccataaccta
    38221 cgatattcgt ataaatatct cggtccaaac tgatgatcgt tgattttttt tgtttcggat
    38281 taactgttac taccatcatc gtatcagaac gtccttgttc cgttcttcct aatccaccgg
    38341 tatctaaacc taatagcaaa accgaaaaag gttctttgtc atcaatgctt actctctcag
    38401 aacgtttgga ggatattcga ttagagtttt tagaaatttt gtttactgtt tggctcgctt
    38461 ctcccataaa tctgattccg tatgctgata atccggcaat cagcaaaacg aataccccta
    38521 acaaggttaa tagaactttt tgccatcgct tcataataca cctctgtttc acattttcta
    38581 aaatattgtc tcatataact gttcaatcag caaattcact ttttaatcat aagaaaactt
    38641 taaaaaacat ttttgtgtaa cgattattat tttttaaaat ttctcaaaat tcttaactat
    38701 cataccatac cgtgaaaaag atcgaaagac cgaatacacg cagttcgcaa tcgttttatc
    38761 tttttttatg tattttttcc agaaaaagcg aacaacaagg tattttccaa ataatcgtgc
    38821 agtaaaatga tcgcacatac ctttcccatc tttttaacag tctttgccta ccgttcagat
    38881 tatcattcga tttcacgaca atttcttcct atgactggtc cttttgttaa acccatcttc
    38941 tattcgtttt atttccagct cactgcatgg ctcttaaaat gactcgaact agtgattgca
    39001 ttaccatcat cttttaatgg atagatatgc tcctcttcaa agtaaaacga ttttctttta
    39061 aagtaataga attgacatgg taatgagtat gtatactagt ggtttcacaa atggtctttt
    39121 attcaacatc caaaaactga aatttcttta aaatccatta ctaacgaaga aaatgttcct
    39181 tgcttgataa ttagttagga catgtcaagt gaacaaatgc atttacttag ccaaaaaatc
    39241 taaaaacttc ataaatagac aaaaagacac gactctctaa gaagtcgtgt ctttattcaa
    39301 caaattatta taatttagtc acttcagcag cttgaggtcc gcgtgcacct tcaacaatcg
    39361 tgaattctac ttcttgacct tcttctaggc tcttgaagcc gtctccttga attgctgaga
    39421 aatgaacaaa tacgtcatct ccgccgtcta ctgtaataaa cccaaatcct ttttcattgt
    39481 tgaaccattt aaccgttccg tgttccataa atgtatatcc tccaaaaaag ttcttgcaag
    39541 taaccttgca agaacattat acccaattta gaaaacgcta gcaagctttc gctcttttat
    39601 tttccaaatt atttttaaga ctgatatgac ggaggaaatg gttattttgc tgcttcgaaa
    39661 cgtttgttta cttcatccca attaataaca ttccagaatg ctgcaatata atctggtcgg
    39721 acatttttat atttcaaata ataagcatgt tcccaaacgt ccaaacctag aattggtgtc
    39781 tttccttcca tcaatggaga atcttgattt gcagtagagg taatagctaa ttttccattt
    39841 tccattacaa gccaagccca tccagaacca aatcgtccag cggctgcttt tttgaattct
    39901 tctttaaaag aagaaaaatc accaaaagct tcattaatcg cttcttttat ttctcctgta
    39961 ggttcgccac cagcatttgg tgccatgatt tcccagaaaa atgaatggtt agcatgtccg
    40021 ccaccattgt tacgtacagc tgtcttgata tctgttggaa tagcgtccat atcagataat
    40081 aattcttcta ttgttttttc gcctaattct gggtatttct caattgctga atttaaattc
    40141 gtcacataag tattgtgatg cttatcatga tgcagatgca tcgtttcttc gtcaatataa
    40201 ggttccaatg cgtcatatgc ataaggtaga tctggtaatg tgtaagtcat aaaaaaattc
    40261 ctcctcaatg ttttcttaat aatgtctaca ttacaaagag taacaaagag aaggaaattc
    40321 ttcaattgtt atgcctagtc gtctttttca ctttgaccta cttcttcttc aaaaacctca
    40381 gtttctttaa aaataaacaa cttgctgaaa atatagttcg caaggataac aataatttga
    40441 gtgatgattt tcgccacata ctcattgctg ttcataacat tgatcaacaa taccattgcc
    40501 cccatgtcca atccgtaaga aaggatacgg tagaagaaga acttaccgca ttcaataagt
    40561 gcatgtgtaa aattcggtga ttttgaatgg aatacccaaa gtttgttcgt tacaaaagca
    40621 aacaatacag aacaaaacca cgaaatagaa ttggataagg ccatacttaa atcaaaatat
    40681 tggcgtgcga gagcaaaact aacaaaattt acaatcgtag ccaaaccacc aaaaaagaga
    40741 tagactagta tctcccaata tccttttgct tctaaatatc cttttaactg tcgatactgc
    40801 ttcatgagcc aacctacctt attaaaaatt cttatttatc atactctata tttcaataac
    40861 tttcatcttt atttaaaaaa attaattttt tttgatcctt tgcctttgtc tctgcttgga
    40921 tcgtcacatg tgtaatacca tattttaatt ttaataaaga aattatacga ggatatatct
    40981 tttcaatctc gcttagtagc tgatcttcaa aatcaatatg tccgtcgaaa ataattatct
    41041 tttcgctata ctgccaaata tggacatgat gaacatcctt taccccatcg attgcttcaa
    41101 tttcttgttc cattttttca tagtcgatag caggtgcact ttgtatcaaa atagcggcag
    41161 cttttttgat caccattatc gcttctatca atatatatat cgaaatgatc agtgtaataa
    41221 ctggatcgat ccagtagata tcgaaaaaac ggatcaaaat tgctccgata atcacactta
    41281 ccgaagacag tgtatcactt aacaaatgca aataactaga ttttatattc aaactttcat
    41341 ctgcttcttg tcgtaataat aatactgaga acaaattcgc gagcaatcca ataactgcta
    41401 ctgttagcat caaatgacta tttatctttt ctggtgaagt gaaccgcttg aatgcttcaa
    41461 cggttaaaaa caaggataat atgatcaata tgaatgagtt gatgaaagca ctcaatattt
    41521 ctaaacgctg gtagccatac gtacgttttt tatttgcttc ttttcgggcc attttttgtg
    41581 ccccgtaaga aaaaataatt gccaatgtat cactaaggtt atggaaagca tctgaaacaa
    41641 gagacaaact ccctgacata atccctccaa taaattctga taaagtgatc accgcattca
    41701 aaaccactac tccaaatagt ttttttgatg atctctgtgg ctgtgtaggc actattcctt
    41761 cctccatttt atttatattg gatagtcttt agacaagaat aacatttttt tgatatttca
    41821 ccaatcattc agctgtttcc ttaaagcact tgtatttcac ttgatacttc gctagaattg
    41881 caagtgatga cactactaga tgaggagtac ttatgacaac aaaacaatta attatcagtt
    41941 cctttatacg attcaaagac ctaaaggagg cgagaaatgt cccattttgg aaagccattg
    42001 tctatctttt agttttgagt ataattatgg ctttgccaat ctcttttcaa gtttttcaag
    42061 tcttaaacaa cattcaattg gatgggcaaa agattgctac tcgcattcct gattttacaa
    42121 tccaaaacgg gcagcttcaa accgaagaaa aagaaggatt catttaccaa acaaactcca
    42181 ttatcttcac atttgatcca gagggaaaac gaacagaaca ggacatctct actgatttga
    42241 tggggaattt tgttagtgtc gggatgttaa aaaacaaact tatcatagct cttcctaata
    42301 caggcacgac ttctgctttg ttgaacaata accaattaga acttccctac accaatgaat
    42361 ctttaaaaaa cttaacaggt aagcagctcc gttctttttt aagtgaagca tctataccac
    42421 tttggataaa agtcctgaca tttatatttt ctatttatcc tagttttctg aatctgctga
    42481 tcactttgct atttaccaac attgctgctt ttctctatgc acgctttcga ctaacaaaag
    42541 ctacttttct tgattgttta aaaacattga tctattcaat ttcattgcct actataattg
    42601 caacaatctt gatggtcttt cttccttctt ttgacactag cgccttcatt gcttttgcag
    42661 gaatctttat ttttgctcaa gctgtcaaag gatgggcaaa aatatcgatc aattaagaaa
    42721 taaagacaac tcgttatttc tacttcaata gaccctttta gaggttgtga catttttttg
    42781 tcacagcccc ttttttattt gaaaaatatt tctttctcaa aaaacaagca aggcgcttca
    42841 aagttcttct tgtatgcgct acacttagag taaagaaagg cgggaaatta actatgtcag
    42901 aaagaatcat ttatgcagat cgtccatttc gtgatgaatt tatcgaagaa atccatacta
    42961 tagcgcctgg ttatatcttt aaaacagaat tacaaccaga ggatctacaa aatgtagaga
    43021 tcagtttggg ctggaactca gactaccaag aaaaactatt agcctcttct accttgaaat
    43081 gggtccaatc tatctcagct ggtgttgatc agctacccct tgagactttt tcaaagaaac
    43141 atattttact ttcaaatggt agtggcatcc acagtgaatc gattacggag catgtcttag
    43201 gaatcatcct tgggtattct cgtggtcttt ttcaagcaca aaaggcacag agtgacatga
    43261 aatggttagg ttcatcggtc cattaccaat ctatccaagg gaaaaaaata ctaatcattg
    43321 gaactggcca tattggaaaa atgattgcaa aaaaatgcaa agcattagac atgaactgct
    43381 atggaatcaa tacatccggt catccagcag aaggaatgga aaagactttt tctttaaatc
    43441 aactaaagga agttcttcct gaagtagatt tcgtagtcaa tatcttacct ctaacggagg
    43501 aaacaactgg gctctttgat cagcatatgt ttgaacactt tgatccaaaa tctgtattca
    43561 tcaatgttgg ccgtggtgct tctgttaaga cacaagatct ggtgcaggct ttaaataacc
    43621 agcaactttc ctttgcagca ttagacgttt ttgaggaaga gcctcttcca caagatcatc
    43681 ctctttggaa aatggacaat gtactgatta cttcccacat tgcaggtttg acacctgatt
    43741 ttcaaaagaa attaatggct atttttttaa ccaatctgaa aagctacttt gccacccatg
    43801 agcttcaaac caatcaagta aaattgtctg caggatatta atagctgaaa acaatatttt
    43861 caaaagaaaa aagagaaggt gacaaaaatt gtgtcaccct ctcttttttt attgtttttt
    43921 aaagtgatag gttggctcct ccaggttggg atcataatca acaagtaaat catatccttt
    43981 aaagaaccat tcatctgctt cctcgataaa aaaaagtgtc ccatccattt ccactttctt
    44041 tacaggatgt tctggctgtt caactgacat tccaatcgaa aaaccatcat gaacctctgt
    44101 tttgccgtag acttttccaa aaaaacgtat tccgtaagtt tctggcagat caatttctga
    44161 gtcaaaccac ttttgtgctt ttggcgttac cgttaatttc atcccaacaa cctactttct
    44221 cttttctttc tattgattca tcttatcgtc tagaatatcc acatcgcctg tctctccaat
    44281 aaccagtaga ttatcatttt cacgaacata ttcatctgct gcaggagaag cgataaccgt
    44341 ttgatctact cttcgtatgg cgactactgt taagtcaaaa cgttgcctga aatttaattc
    44401 agctaatgtt ttgttgaaaa atttaggatt ggagactcgg atttctgcta aagaatattt
    44461 atctgacagc tctaagtagt ctagaatatt ctttgatact aagttatgtg caatccgtat
    44521 ccccatgtct cgctcagggt gtacgacacg atctgctccg atcttctcta gtacacgggc
    44581 atggtactca ttttgggctt ttgctaaaat attcggtacg cccatttcct ttaccatcag
    44641 agtaactaag atgctggctt ggatatcttc tccaatagcg acaattacat gatcgaaatt
    44701 tcttatacct aatgaacgaa gtgtcatctc atcctgtgca tttgccacaa cagcatgggt
    44761 ggctatattc atatattcat tgactcgatc ttcattactg tcaatagcca gtacttcttg
    44821 tccagcttcg atcagtgtac ggcagatgct tccgccaaat cgtccaaggc ctacaattgc
    44881 aaaattttgt ttcaccattt tcctccttta gtatcttctt ttctcagtat aacaaagcct
    44941 ttccaaaaac aaaagcaagc atcattattg taaaaaagcc aagaaaaata atagttattt
    45001 tctcagcttt tgattatcct ctttatcatc gtttttattt caccaaacca tgtttaaacg
    45061 catagatagc tgcttgagtt cggtcttcta cctctaactt agctaatata ttcgacacat
    45121 gcgttttgac tgttttcagt gtgataaaca attcatccgc gatttcctga ttacttttcc
    45181 cttcagaaat cagcatcaaa atttcatttt ctcgattcgt taattcttca tgcaatactg
    45241 gatcatttcg ccgactcatt ttttccatca tttttgttgt cacttcaggt tctagtactc
    45301 gctcgccgcg ttgcgtagcc cttatagcat cagctatttc atgtgctgta gacgtcttca
    45361 acaaatatcc tgctgctcct gcttcaattg caggataaac tttttcatca tcaataaaac
    45421 ttgtgacgat aatgattttg gcttttggcc aatcttttaa gattgctttt gttgattcga
    45481 tgccatccat ttcttccatg actagatcca ttaaaatgac atccggtcga agtgccatcg
    45541 ccttttcata gccttgacgt ccattttcag cttctccaat cacttcaatg tcttcttgga
    45601 tagataaata agaagagacg cctaagcgta ccatttcatg gtcatctact aataaaactt
    45661 ttatcatctt tatctatgcc tccttcatca aagggacctt ggtctccacg ctcgtacctt
    45721 gccctttaaa actaatgatt tttactgttc caccaatacc agctactcgt tctttgatat
    45781 tgttcaatcc ataacttccc gttttggttt cattcatatt aaatcctgtc ccgtcgtcaa
    45841 tgatacgtaa taaaagattg ttatctattt tatgcaaata gacttctaat tcattagcct
    45901 tagcatgtct taatgtgttt gataacaatt cttgaacgat tcggaacaag tgatcctcaa
    45961 tggaactagt taattttaca tcttctacct cccattttag cgaaatctgg attttattct
    46021 gcaattcctt caataactgc tcaatgcctt gttttaaact tttttcttct aaattgactg
    46081 gacgcagatg aagaagcagc gcacgcatct cagactggga agcgttgatg atctctgcca
    46141 ccattttcaa ctgcttacgg aacatttctg gtgtctcact tttttctgct tgctccgtca
    46201 atgccgacat catcatcatc gctgcaaata attgctgtga aactgaatca tgaagctcac
    46261 gagctaatcg atgcctttct aattttaaaa tctcttcttt ggtttgtcca tctacatatt
    46321 gaggacggct agtcataatc tgtaattcac tggatacttc gatcattttt tcttttattt
    46381 tagtaatttc catatcaata tctttgatgt acagatcatc cgacgcgctt gggatcggct
    46441 gatgcaaaag tttggattca taattgccag aagaaagtgc acgaagcttt tcttcgattt
    46501 tcccgtattt tgccttttga ataagtgata acaatagaaa tacgatcaat cctacaccta
    46561 atgaaattgc catcaaatgg aagataagcg gaacgtataa taaacgcgca cgcagcaatt
    46621 ctccccacca atgactttgg ttactggcat aaaaataagt gaaaagtgaa aatagaataa
    46681 taaggaaagc cgcaatcccc gaataaacag ctagcattgc tttggatatt cttcccatca
    46741 tattcgtatc acctcgatat ctccaaccaa agtattggta atgatcttca atctgcgagg
    46801 attttcatca taatcactgc tgcagatttt gatctgttcg ttttttaagg aaaactgctc
    46861 ttcttcaaac aagacatttc ctactaatgt cgcatgctct aaacgaatag ccactcccag
    46921 gggtactaag atccgtgttc tgcctatccc ttttcttaca atcacaatat tatcatcttt
    46981 tggcaaaagt gtattgccca aatcgatgat agtatcacca gaaatgatgg caatgttgat
    47041 atcatcccat tcatagacgt cgtttccaat acgttggtta ccaaataatt gctgtctttt
    47101 acgctcatta ttatgtgttt ttaattgctc tgtttggacc atcatgatct gtttttttcg
    47161 ccaaaaagca ttttttgtca aatctattcc tgaaatctct acacctttca gcccgataaa
    47221 taaaacggcc aatacaacca tcatccataa agcaggactg ttgactaagc taaaaaaaat
    47281 gaccaagctc cctaagatca attgaaaatt ttgaaattta gtacgaggat atttgcgcat
    47341 agctagatat atattgaaaa taccgaatat tactaaaagc aaaagtccag tattgtttac
    47401 gatttgccaa acagcaaata acagcagcag tgcttcgact accacaaaaa aacgccaaga
    47461 actattcatc gctttttctc cctcctgaca cattctcatt tcatcatacc tatttttttg
    47521 actgttggct ataggacttt agatgtaaga tcgttcagtg aaacctgcaa tactcaaaga
    47581 gaaaagatct agagtgcgtc atcagtaaga tgaactctct agatctttca gtcagactac
    47641 gcttgttcta ctttgacgat tttaactaac atatcgccac ctggtgtagc aatggttact
    47701 tcatcatttt tacgtttgcc aatcaatgct tgcgcgatag gagaatcatt ggaaatcttt
    47761 ccagaaaacg ggtctgcttc agcacttccg acaatcgtgt actcttcttc atctccatca
    47821 ggcaactcga tgaacgtaac tgttttaccg atcgatacct catctgcatc tacgccgtca
    47881 ttatcgatga tttgtgcaaa acggatcata ttttcaattg ttgtgattct tccttcaaca
    47941 aatgcttgtt catctttagc tgattcgtat tcggagtttt cggaaagatc tccgaaaccg
    48001 cgagcgattt tgattcgttc aatgatttct ccgcgtttaa ctgtttttag ttcttctaat
    48061 tctttttcta atttttcttt accttctaga gtcattggat aaactttctc taccatgtgt
    48121 aacgtctcct tttttcgata aaataccgca aaaggtaatc ttttgaggaa tatagtccaa
    48181 aaaagattac cacgcttttc ttaaaatgta aatgtttttc taaaaatgat caatgactag
    48241 cccctgtttt tctaagaatt atttacatat ttttgcacta gttccatatg ttcttcatac
    48301 gttttagcga aataaacatt tccagtgctc gtatctgcta cgaaataata atagtcattt
    48361 tctgctggtt ctaatactgc cttgattgcc tcttcgcttg gattgtcaaa aggaccaggc
    48421 ccatagccgg tattggtata caaattgtat ggtgaatcta cagctgtatc ttcatacgtc
    48481 accaattctt tatgttctcc taatgcatat aaaacagaga tatctgactg aagtggcata
    48541 tttgccttga ttcggttaaa gaagacttgt gcaatatttt tacgatcatt ttcttttacg
    48601 ccttcttttt caactagaga agccaaagtt aaaacttctt gtacagtcaa atttttctgt
    48661 ttgatagctg aataatagtt ttgcatgacc gtatttgttt tgttcaccat ctcaatcaca
    48721 agatccttaa gcgaagtact cgtgtagtaa tcataagtag ctgggaataa gtagccttcc
    48781 aaacggtatt ttgtgttctc agctttagag gcactttcca acaattctgg gaatttttga
    48841 tacaactcgt taaagaaagt ctcatcattt actaaatcta aaaattcttt cttgtcttta
    48901 ccagttacct tcgctacccg ctcagcaatc tgatcgatgt cgtagccttc tggaatggca
    48961 attttcccat ctgcaacttt tgttggctca gatgtgccgc cttcttgcaa ctgcttgcca
    49021 atctcatcaa gagtcatatt aggcgctaac tgataataac cagcttgaaa gccagttaag
    49081 tttttaaatt ttgtataata attgaaaaca atcccacttt taataatatt gtctttctcc
    49141 aaaatttcgc caatctgctt attagaagag ccgcttggga tttccacttg gactaattgg
    49201 tcgtccgatt tatctaaagg cttcaacccg ctatctacat aacgatatac ggtaaacccg
    49261 agaaatccgc caataatcaa taatgtaaga gcgatcacta aaatgatctt cctcacgatc
    49321 ttatcttctt ttttacgcag atttctccgc ttgtcgatta tatctgctgt agctgcctta
    49381 ctggcttctt ttttgtcttt gcttgggtgt tgtttgtttc ctgacaaaac aactcctcct
    49441 tactatcatc ttcgtaactc tgtttattat acatgaaatt tctac
//
LOCUS       DAZUMK010000008        48456 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000008 DAZUMK010000000
VERSION     DAZUMK010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.008"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..333)
                     /locus_tag="ACG808_000473"
     CDS             complement(<1..333)
                     /locus_tag="ACG808_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046131.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFE"
     gene            complement(508..2643)
                     /locus_tag="ACG808_000474"
     CDS             complement(508..2643)
                     /locus_tag="ACG808_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFS5046132.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(2726..4381)
                     /locus_tag="ACG808_000475"
     CDS             complement(2726..4381)
                     /locus_tag="ACG808_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS5046133.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(4383..4697)
                     /locus_tag="ACG808_000476"
     CDS             complement(4383..4697)
                     /locus_tag="ACG808_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFS5046134.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            4867..5127
                     /locus_tag="ACG808_000477"
     CDS             4867..5127
                     /locus_tag="ACG808_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFS5046135.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            5141..5455
                     /locus_tag="ACG808_000478"
     CDS             5141..5455
                     /locus_tag="ACG808_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFS5046136.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(5940..6494)
                     /locus_tag="ACG808_000479"
     CDS             complement(5940..6494)
                     /locus_tag="ACG808_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFS5046137.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(6620..8548)
                     /locus_tag="ACG808_000480"
     CDS             complement(6620..8548)
                     /locus_tag="ACG808_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFS5046138.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(8854..9570)
                     /locus_tag="ACG808_000481"
     CDS             complement(8854..9570)
                     /locus_tag="ACG808_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFS5046139.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(9571..11394)
                     /gene="glpO"
                     /locus_tag="ACG808_000482"
     CDS             complement(9571..11394)
                     /gene="glpO"
                     /locus_tag="ACG808_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFS5046140.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(11408..12904)
                     /gene="glpK"
                     /locus_tag="ACG808_000483"
     CDS             complement(11408..12904)
                     /gene="glpK"
                     /locus_tag="ACG808_000483"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFS5046141.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(13040..14500)
                     /locus_tag="ACG808_000484"
     CDS             complement(13040..14500)
                     /locus_tag="ACG808_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5046142.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(14490..15785)
                     /locus_tag="ACG808_000485"
     CDS             complement(14490..15785)
                     /locus_tag="ACG808_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS5046143.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVKEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(16117..16794)
                     /locus_tag="ACG808_000486"
     CDS             complement(16117..16794)
                     /locus_tag="ACG808_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFS5046144.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(16824..17915)
                     /locus_tag="ACG808_000487"
     CDS             complement(16824..17915)
                     /locus_tag="ACG808_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFS5046145.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(18016..18450)
                     /locus_tag="ACG808_000488"
     CDS             complement(18016..18450)
                     /locus_tag="ACG808_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFS5046146.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(19034..20491)
                     /locus_tag="ACG808_000489"
     CDS             complement(19034..20491)
                     /locus_tag="ACG808_000489"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5046147.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENL
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIEDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(20488..22383)
                     /locus_tag="ACG808_000490"
     CDS             complement(20488..22383)
                     /locus_tag="ACG808_000490"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS5046148.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(22441..24216)
                     /locus_tag="ACG808_000491"
     CDS             complement(22441..24216)
                     /locus_tag="ACG808_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFS5046149.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYLVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESSEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(24266..25762)
                     /locus_tag="ACG808_000492"
     CDS             complement(24266..25762)
                     /locus_tag="ACG808_000492"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5046150.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     EIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRIWKEEDCAPVMLDGDEQYLQANTVDYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(25956..27578)
                     /locus_tag="ACG808_000493"
     CDS             complement(25956..27578)
                     /locus_tag="ACG808_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFS5046151.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(28028..29341)
                     /locus_tag="ACG808_000494"
     CDS             complement(28028..29341)
                     /locus_tag="ACG808_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFS5046152.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCILMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLLGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFMGFMWILNVK"
     gene            complement(29586..29810)
                     /locus_tag="ACG808_000495"
     CDS             complement(29586..29810)
                     /locus_tag="ACG808_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046153.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(29830..30252)
                     /locus_tag="ACG808_000496"
     CDS             complement(29830..30252)
                     /locus_tag="ACG808_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5046154.1"
                     /translation="MESVYRQERKSNRKKGQTMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGVLTFNFSDPEGNYFVIGKAESSD"
     gene            complement(30394..31104)
                     /locus_tag="ACG808_000497"
     CDS             complement(30394..31104)
                     /locus_tag="ACG808_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFS5046155.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGDESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(31122..31618)
                     /locus_tag="ACG808_000498"
                     /pseudo
     CDS             complement(31122..31618)
                     /locus_tag="ACG808_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(31655..32107)
                     /locus_tag="ACG808_000499"
     CDS             complement(31655..32107)
                     /locus_tag="ACG808_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS5046156.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(32242..32847)
                     /locus_tag="ACG808_000500"
     CDS             complement(32242..32847)
                     /locus_tag="ACG808_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFS5046157.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(33297..33917)
                     /locus_tag="ACG808_000501"
     CDS             complement(33297..33917)
                     /locus_tag="ACG808_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5046158.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(33919..34788)
                     /locus_tag="ACG808_000502"
     CDS             complement(33919..34788)
                     /locus_tag="ACG808_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFS5046159.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANKAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(34811..35218)
                     /locus_tag="ACG808_000503"
     CDS             complement(34811..35218)
                     /locus_tag="ACG808_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFS5046160.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDRHKLAELYEQVGLKFSELHENDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(35237..36526)
                     /locus_tag="ACG808_000504"
     CDS             complement(35237..36526)
                     /locus_tag="ACG808_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS5046161.1"
                     /translation="MSVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINDQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     ILLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(36553..36855)
                     /locus_tag="ACG808_000505"
     CDS             complement(36553..36855)
                     /locus_tag="ACG808_000505"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5046162.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(36880..38973)
                     /locus_tag="ACG808_000506"
     CDS             complement(36880..38973)
                     /locus_tag="ACG808_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS5046163.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLEEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(39462..40190)
                     /locus_tag="ACG808_000507"
     CDS             complement(39462..40190)
                     /locus_tag="ACG808_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFS5046164.1"
                     /translation="MGLCLFALPTQGFAVAGGHGGGTSSGRSSGRGSFGGRSYGGSYF
                     GSRSYGRDSYGYYGPSTGPSSYEQTGFLALAVLSVGGVSAFSIVKNKRQKKRLKSEIL
                     SRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFAEHQKVLQKNKAD
                     GVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLISGSKTQRQYFSQ
                     IWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(40336..41049)
                     /locus_tag="ACG808_000508"
                     /pseudo
     CDS             complement(40336..41049)
                     /locus_tag="ACG808_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
     gene            complement(41050..42957)
                     /locus_tag="ACG808_000509"
                     /pseudo
     CDS             complement(41050..42957)
                     /locus_tag="ACG808_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
     gene            complement(43071..44402)
                     /locus_tag="ACG808_000510"
     CDS             complement(43071..44402)
                     /locus_tag="ACG808_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS5046165.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRVMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKANFGQLNELVPFGFAGMIASFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKNAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQSGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFVGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(44368..45846)
                     /locus_tag="ACG808_000511"
     CDS             complement(44368..45846)
                     /locus_tag="ACG808_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS5046166.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLKNPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(46151..47986)
                     /gene="tdc"
                     /locus_tag="ACG808_000512"
     CDS             complement(46151..47986)
                     /gene="tdc"
                     /locus_tag="ACG808_000512"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFS5046167.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEQVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     EQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKEIAKAMEKKLNEIIQ"
     gene            48213..>48456
                     /locus_tag="ACG808_000513"
     CDS             48213..>48456
                     /locus_tag="ACG808_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5046168.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIK"
BASE COUNT        13889 a        10404 c         8091 g        16072 t
ORIGIN      
        1 ttcaaaagat ttatttactt ttttgtatag atttcgattg ttccacctag tcaatacaaa
       61 tgaggtaatc aggaaactga aaaaaatcaa tacaaaaaag ccgattaaga aatacagcat
      121 tttcttttct gataaaaaag cgtacaagga aaaatccttg gctctttctg gtctcgttaa
      181 tgggaaaaag acccatataa aaggaagaac aacgatcatc catcctaaaa gagccactaa
      241 cgtttgccaa atctttaacc agtaattacc ttttgaaaaa tacttatctt tcaccaaaat
      301 tgatgcaact tcttctcttg ttactttttt catatcctca cctcacgttt ttttgttacg
      361 acgtaggagt ttaccataca tttcatttct tttcacataa agtgttccta aatttttcct
      421 aagaaaaaga acaaaacatt tctcatcagc aaatgaaaaa tattttgttc tttttatcag
      481 ttgttctttt tattttttat ctcttaatta tgttgctctt tccaatacga caatgctttt
      541 cattcctcta gatatcgccg tgtacatttg tctgcgtctt ctttgctcat ctccatcgtt
      601 ttcaaatgga tataagatta catgatcaaa ttccagtcct tttgctagac tcaaaggaat
      661 gatttggcag ttttctaatc catatgaatt ttttttaatc gattcttgta gcgtttgggt
      721 ttcctcttct gtttgggtga taactgccgc tttttctcct tttagagaaa ccaaaatctc
      781 agctagtatt ttcaacaatt cttcagagga tcgaaaagag cggtattcgg gttcttttcc
      841 ttgcggtcgg atcgatacga tgtcgatctt ctcttgatcc acagcatagg ttttgaaaag
      901 ctctgtgatt gctccgctag aacggtaact atttctaaga tcataccgat ggatcggcag
      961 atggtagtca tcgaaacaac gcataatatc agcaaaagta gtggaagaat taaagattgc
     1021 ctgattctcg tcacctacta aagtaaagtg agtttttgga aataattcga tcaatagacc
     1081 taattgtgcc agggtataat cttgtacttc atcaatcaat agatagcgaa atgctggtac
     1141 atttaccttt tcaacaagta aatgacgaat agctaatata atgactgctt catcaacagt
     1201 acgtgtgggt tgccaagcat aagctgagcc atatcgtttt tcataaattc tttcaaacaa
     1261 ctgatgctca tccacccatg ccatttcttc gatttgtctg gttatttttc gatatttttt
     1321 ttgtaaaagt ttccttgcat aagctacaat actttgttcc gaatcatcag aaatcaattt
     1381 accgaataat tccaactgcc tatcttctgt caacgagagt acctgattct gcacagcagg
     1441 actttttgct tgtttcaaaa gatgattctt ccattcgctc atcagtgcct ttttcgttgc
     1501 ttgaagccta tcgatcaatt taggatgagc aggaactttc tcatatattt tttcgatcgt
     1561 ttttttagaa aggactgttt ttccattttt agtaagtcca cggataaaat tcggatgatc
     1621 taaaaacaaa ggatctgatt gttttagatt atcgataaac tttttgctac gcaaacgttc
     1681 tgtctgctcc gatacatttt ctcccgtgat acgtttgaaa taagcctctt caccttctat
     1741 ttcctctgct gaaagctgtg aaaccagctg tatgatggtt agattcagtg gatttctttc
     1801 acctaatgat ggtaggacat tcgaaatata gtcgataaac cgatgattag gtgatagaat
     1861 cagcaagtca tcggatgtca tcgtatctcg atataaatag agcagatacg caatacgctg
     1921 catgatagca gaggttttac cacttcctgc tacgccattt accaagatat tttgactttt
     1981 cacatcccga atgattgtgt tttgttccga ttggatcgta gcagtgatag ccttcatttc
     2041 attggtctca tcttggccta agacttctaa taatacgtca tcttgaatag ctacagacgt
     2101 atcaaaaaac ttgaccaaac gatctctttc caaaagaaat tgtcgtcttt ctttaataac
     2161 cgtatcgatt ttattttgac gaaccgtata agaagatgga ccaatcatgt tattgtaaaa
     2221 aagttcggca attggtgaac gccaatcata aacaagattt tcatctgcat catctgtgaa
     2281 atttgctgta ccgatataaa aggattcttt tggctcatta tccagaaagt ctacaacgac
     2341 ttttccaaag tagggacttt tcaataaacg ttcgatcttt gctagctgat tttctgcgtt
     2401 tttctgtttg atattcatct gatcgatttc tctatttttc atttcaatca tcgaaaaagt
     2461 atccaggtta tctaagtaac tgtcaaaatt caatcgaagc tcgccagcca tttctttcaa
     2521 cgactggttt ccttctattt ttgccgtatc catcgattcc cgcagttcgt tttttttatc
     2581 aattagcttt tgatacacgt tcttcagata tgcttcttct aactgttttt gtgtgttgac
     2641 catacacatt ctcctctttt tttaaagtgg tggaaccgat tagtttacct cttttaagag
     2701 tcgaatgcaa gctttttaac gagtatcatt tgttaggata tatcagttct tcaggtcgga
     2761 cagtttgata taaagaaaat gcaccatcaa ggttttttac gtggtagcca gactgcttca
     2821 agattctttc cgctagatag ctacgtaatc cgctatgaca gctgacgata tattcttgtt
     2881 ggctgtctaa ttcatttaat cggtcccgaa gttcattcaa agggatatgt ttggcttcct
     2941 tgaatttgcc cttttccaat tcttctgctg ttcgcacatc taataaaatt tttccttttg
     3001 ctagttctgt cggcaattca tgccattgta cggtttcact aagcccttct gcaatgttca
     3061 ttgctgcata tcctagcatg tttactggat ctttggctga gccaaatggc ggtgcatacg
     3121 tcaattctaa ttccggtaaa tcgaagatcg tcaaatgccc tttgattgct gttgctaaaa
     3181 tatcgatccg tttatccaca ccttttgccc caacaccttg tgcaccataa atctcgccag
     3241 tctctggatg gaagatcaac ttcagtaaaa tatctgttgc ttctggataa tagcttgcat
     3301 gatcttttcc tgatacatga atgacttcaa aagacagttc gttttctttg gctgttcttt
     3361 cacttaaacc ggtggatgcg gcagtcattc caaacgttcg tataattgct gttccaatac
     3421 cgccttgatt tcttcgagaa attccggaaa tcgtgtccgc aacttgtcta ccttgacgat
     3481 tggctggtga agcaagagaa ataagtgcat cttttccagt gatttgctgt tttacaacga
     3541 tagcatcccc caccgcataa atatcaggaa tattcgtttg ataacgttcg tctactagga
     3601 tacccccgcg tatgcctaat tcgattccag cggtcactgc tagctggtta tcaggacgaa
     3661 caccaatgga taaaataact aaatcagcag ggatggtacg cccatcatcc agtcgcaatc
     3721 gtttcccgtg gtcttcaaat ccagcgacag catgagaagt aatcacttct actccttgat
     3781 gagacaattc tttttctata aaagctgcca tttcttcatc cagtgttgga agaatatgtg
     3841 gtgcttgttc gatcaccatg acttctaaac ctcttctttt taggttttct gccatttcca
     3901 atccgatgaa tcctgcacca acgatcacgg cacgctttgg ctgtttttct aatgcatgca
     3961 tcactttatc gatatctggt acatttctaa tagaaaaaac attgtcggct tctgttatcc
     4021 ctggaatcga tggaacaact ggttttgcac caggagataa aaccaatgca tcgtactgtt
     4081 caatgtgttt ttgaccctca tgtacgactt ctaccgtttt gttttcggga aagatggctg
     4141 tcacttcatg atttggacgc acatctaaac aaaaacgagc ggataatgat tctggcgttt
     4201 ggacaagaag attttcccgt tcactgattt ctcccgaaag ataataaggc aagccgcaat
     4261 ttgcaaaaga aacatacggt cctttttcaa aaacaacgat ttctgcatct tccattaatc
     4321 ggcgaagccg tgtagcagca gacattccac ctgctacacc tccgacaata acgattttca
     4381 tcttatttcc ctcctactgt tcgatcaaac cattgattca tgccaccacg tacattcact
     4441 acatcataac cagatttctt taattcttta gctgcttgtt tactcctcat ccctgattga
     4501 caaatcacat agacggtctt ttctttgtct ccatcatatc tgtttatact ttgtaatgga
     4561 acatttttag ctccttttat atgcccgccc cggtattctg caggtgttct aacatcaagt
     4621 aacgctggat tttccaaaag cttttccttt aattctggga ctgttatcga ttttattgat
     4681 tgaaatagca aattcatttt ctgcacctca tttacatttt tataaatacc tcatggggta
     4741 tattaaacag gagctttatt aatatgtcaa ccagaatgac tcttcatttt tttcactact
     4801 ttttatattt gcataaaata ccgataaggg gtattatcta attataaaaa tagaggagaa
     4861 tgtgttatgg agattgataa taaaaatatc ctgaaccgtt tgaaacgtac agagggacaa
     4921 attcgtggta tccaaaaaat gatcgaagac gaaaaagaat gtatggatgt catcactcaa
     4981 ttaagcgctg ttcgttctag tattgatcgc gtaatgggca tgatcgttgc agataatctg
     5041 aaaaattgct ttgagaaacc tgatagtaat ccagaagaac aagcggctaa actagaacaa
     5101 gcgatcaaga tgattattaa aaagtaaagg aatgatttca atgaaagtcg ttttccatat
     5161 tgatgaatta gaaaaatggt cagaaacagg gaaaaacgta aaaaacctga taaaagcttc
     5221 tccagaaacg acgattgtcg tatctgtaaa tggcatcgcg attaccggtt atctagactc
     5281 tgccaatgca gaattcttag atttgcaagg agtcgttttt cacgcttgcg ccaatgctat
     5341 gcgtgctaat catatttcag aaagttcttt acctgaacaa gtaatcgttg taccagcagg
     5401 tgtccttgat ttagttgaac tgcaatccca aggttatgct tatatcaagc cttagtcagt
     5461 agaggttgcg acagaagtga tcagcttcaa gaaatttcgg cttatttttc gaagaagcta
     5521 cttctggaaa accgtgaatc aaaggttgtg aaaacgctgt cctgcatcaa gtaataagaa
     5581 cagccaaaca aaaatagctt ctcatatttt tcgtttggtt gggcttatta ccgcagatgc
     5641 aagcttttga acaccgttta aggaagaggt tgtgaaatca gcggtttgac ctaagtatta
     5701 gagaaaaaaa cgggaaaata gctcctcata ttttgccgtt ttttgagcta ataccgaagg
     5761 tcagctgatt gaacaccgtt taccaagagg ttgtgagcct accatttaac tccgaatcac
     5821 aagaaataat ttcaaaaaca acttctcctg tttttggttt ggttggctta ttatcgagaa
     5881 gtagctactg ggagtctatt tatcaaaaaa aggaatgcga cataggtcac accccttttt
     5941 tattttcttt gaaacatctt tcggtactcg acagcataca agacaaaaga ttgtaaaaga
     6001 ccgtaagtga tgacggcaaa aatgactctc acccacagcg ttggtaattc tgggaaaagc
     6061 agcaaaagga tcatgccaac atagatgaca gcagtcgcta ctttgccata ccacttcgct
     6121 ccacttaatt tgatattttt agatagcccg attaatccag ctacaaacat aaagccttct
     6181 tttatcaaga atagaccaaa caacagccaa agccacggtc gataccatgc catagacaaa
     6241 atcagaaaaa gttgtgtcaa tttatctgca aacggatcaa gcaccttccc taagtctgtt
     6301 acctgattat atctccgagc aatgaccccg tctagataat cagacaaacc tgagacaaca
     6361 atgaccgctc ctgctatata aaatgactgt ctcagcacaa aataaagata aatcggcagc
     6421 aaaaaaatac ggaatatcga taaaagattc ggaatcgttt gtatttcttt acgccagtct
     6481 gtgcttaatt tcattttatc gccctacctc ctcagcactc ttttcttttt attttagctt
     6541 acacggggaa taaaaccaat gatttcactg tttttatttt tctttacagc cactgatatt
     6601 atgagctaat ttgagataat cacttgctgt tttcatcgtt tactcttctt tcttcctgtc
     6661 gtttgcgcat ttcttttact agtactttat gagctttttt tgatcctcgc tctaggtcac
     6721 gctgccgttt acgtgtttta taatcgataa acagactgtc taaatcgtaa cctgtcgggt
     6781 acagatcttc tgctgaacgc accagacgaa cacgctgacg aggcacgtgg attatttttt
     6841 tgtctaaata tacatcaatc gtatcttcac ctatatcttc ataaactaac gcttcttttt
     6901 ggtattcatt gacaaatatc cgatcacctt ttgcaaagag ctggcgttct tttttattct
     6961 ccttcctatt tgtaactgct gtcttaggaa gaaattcttt tttctgtgtc cgataggctt
     7021 tttccgccaa gtaacgctct gcttgatgga tcaatttcat cgacatatcc attttatgag
     7081 caatccacaa agcttggctt tctcctgttt ctccaacccg caactgatat ttaggacgaa
     7141 gtgcttcccg atcgaaagcc atagcagccg gcacaaaatc ttcatgatct ccggcgaatt
     7201 tcttgatttc tccataatgg gtcgtggcaa tgatcaatgc cccttgctca tacatagatt
     7261 ccatgattgc gatcgctagt gcagcccctt catttggctc tgttccactg ccaatctcat
     7321 ctaaaagtac caacgtattc ctttttatct ttttcaaaat agcagcgatg ttttgcatat
     7381 gaccagaaaa tgtactcaac gcattttcta aattttggtg gtcaccgaca tcgacgaaaa
     7441 tctcatcaaa gacagcaagc tctgtccctt cttttgccgg aacttgcata ccgaacatcg
     7501 ccatcaaagt caataaaccg actgtcttca acacgactgt tttgcctccg gcatttgctc
     7561 cggtgataat caagcctcga tagtctttac cgatttcaac atccaatggt accgcatgct
     7621 caggtaaaaa tggatgtctg ccttgcttga taatgatatg ttccgattta ttgattctag
     7681 gtgtgcttcc attgatttct cggctaaatt ttgcccgagc aaagatgata tctaacaccg
     7741 tgatcgtttc gatcaataaa tcgatcactt cttcgttttc tgcgatagca ccagtcaatg
     7801 ccgccaggac ttgatattct tcactgattt cttcagcttt taacaactga tagtgttcat
     7861 tcaatttaga aatcacagtt ggttcaataa aaaccgtcgt tcttttattt gattcatcga
     7921 taatcgttcc atctatttta tttttatagc tggctttgat aggaatcgta tagtattcgc
     7981 ctttttgaac aatcattgct tcttggatca tttctttgtt tttcggatga cgcaagaatt
     8041 tcagtaattt tgactggatt tctttttcga ttagttgtaa ttgtttgcgt actttacgca
     8101 aattacgtga cgcgtcgtca ctgactttat tattttttat tttctggtca atcaactctt
     8161 ctacatttat caaatcagga agatgtttgc tataagcaaa caacaagggt gtctggtatt
     8221 gattcttatc aaaaaacttt gtgatcatcc gactgcttct aagaaaatcc gcatattcga
     8281 tcaaatcggt tggctgcaga atcaaccctt ttttgacttg ttctgtcagt gcatcgatcc
     8341 tcggcaaccc cataaatgga acatgctgac tgctttctaa gatcaagcgt gcttctcttg
     8401 tttcttcttg acgatccaag acagtttgta ggtttgtcga gaccgtcata tcgctgattc
     8461 gttttttgct atagtttcca attgctcttg cttggatttc ctttttgatc cgatcaaatt
     8521 gtaattgact aatcatttcg ttgtccatat tctctccttt tcttgaagag atacctgtcc
     8581 caaaataaat aagggcctag agataaataa aaggatagaa cacagcttaa aaaagtcatg
     8641 tcctatcctt attagaatac aaagatattg ttttcctgac aggtaccgtt tcataagcac
     8701 accaaataaa ctttgtttat tggctattcg acttactgat tagataccag ctacactcac
     8761 aaaacatacc ccattcttct aggtcttatt tattttccac ttaactatag caagacacat
     8821 tttggttgtc aaactttagt atgttttata gatttacacc attaatgaat agacacctac
     8881 cgcgataatt gctccgatca ttggacctgc gattggtacc catgaatatg cccaatctga
     8941 agttccttta ttagctattg gcaataccgc atgtgctaat cgaggaccaa agtcccgagc
     9001 agggttgatt gcatatcctg ttggccctcc taatgaaaga ccgatggcta agatcaatcc
     9061 acctacagcg aatacgttcg ttccaggtgc aaattcgttt tgtccaaaag ctagcaaccc
     9121 aagaactaat acaaatgtac caattaattc tgtgatcaca tttgctggat aattccgtat
     9181 agcaggacct gttgcaaacg ttcctaaaat cgcgctttca tctttcgttg cattccaatg
     9241 tggcaaataa gaaagccaaa caagaatggc ccctgcaaaa gctcctaaaa cttgcgcaat
     9301 gatgaaagga acgacaagat tccaactaat tgcccctgtc atcgccatag ctagagagac
     9361 tgcaggattc aaatgagctg ggcccatgaa tccagaaata tagacagcca ttgttacagc
     9421 gagtccccaa ccaaatgcga tgacaatcca gccagaagcc tgtgctttac ttttgtttaa
     9481 attcacagcc gcacacacac catctccaag caaaactagg atcagtgtcc caataaactc
     9541 ccccatgatt tgagtcatat cactgttcat ttatttctcc ccctttagtt cccgcagatc
     9601 tgattctgca atcaatgctt ctaaacgttt agtctgttgc gccttctcct cttctgtcca
     9661 ttcaaaatat ccagcaattg catcgatcac aggttgcttg atctcatcaa gctgatctcg
     9721 ttcaaacaac agatgatttg tacgacgaat gagataatcg cctggggcca aaaccatttc
     9781 ttcttctaaa ccataacgta accgagctga ctcagcgaga ctcaaaccag gataaggtgc
     9841 catttctttt gctaattcaa agatacgtcg ggcattagtc ccgtaaaaat cagcgatata
     9901 agtagcatct tcttcctcta aaccggctgc tactccttct tttgctaatt ctgtcactgt
     9961 ttcttctaat ttagttggat cgaagtttcc acctgaaatc tgatattttt tagagtcgat
    10021 ttctttcgtc tctattccgt attcttcttt taataattgg cgtatcaatc gcaaagctcc
    10081 ttctgccatc ttacggtaat cggtgatttt cccaccagat aaagtcagca aaccatctgg
    10141 ttctctttct aaagaactac ctctggaaat cgtagaaggt gctttttcat cacggctgtt
    10201 ttccaaatgg ttcaacacgt cttctacttc agcacgagaa actttgtttt ccttatattc
    10261 acttactgta tcgacaactt tattgaagct cttgtctgaa atcgatccat tatctccacc
    10321 attgtaatca gaaccagaat taccaatcaa cagtggacga agtcctgccc agcttgcttc
    10381 gatatcattc aatgtgatat ttgcttctgg atagcgatga ttgatcacat ccaatagata
    10441 atctacatct tcttgtgtga ctttcggatc cgtaaaatct ccttggtaat ccgtatctgt
    10501 cgtaccaaag taagtcttgt tttctcttgg aatagcaaaa accatccgcc catcttgctt
    10561 tcctgtatcg aagtatgtgg gttgcggtac aggcagtttt ttcgcatcta caactaaatg
    10621 gatcccttta gttggacgca ttttaggaga gactggacgc gtaaaattca agtttcgtac
    10681 tttatctacc caaggaccac tcgtattgat cactaatttt gctttgattt cgatcacttc
    10741 atctgtcagc aggtcacgag ctttgacgcc aacgatttga tcgccatcat ataaaaagcc
    10801 aaccgctttc attttactta ctagataagc cccatcttcg gcagcctttt tgatattatc
    10861 aatcactaaa cgggcatcat tgttgcggaa atctagatag acacccgcgc cttttagccc
    10921 ttcttttttc aaaaacggtt ctctttccaa tacttcttct ggtgtcagcg tataattctc
    10981 atatttggtt cctgtcacat ttgccagttt gtcataaagg tccattgcta ctttgaccga
    11041 gaacatattg aaggttgttg ctccttcgcc ctcatagatt ggcaaaagca ttggatctgg
    11101 ttttgggatg tgtggggcaa tactttgtac gactgcacgt tcaccaactg tgtcagctac
    11161 tacttccaca tcaaatgtct tcagataacg aataccgcca tgaacaagtt tggtcgagcg
    11221 agaggaagtc ccttctgcaa aatcctgcat ttcgatcaat cctgttttga ttcctgatgc
    11281 tgctgactgg actgccacac cggcaccagt gattccgcca ccgatgatca acagatccaa
    11341 ttcttcttgc tgcatttttt gaatgctatc ttgtcttgtc ttgtttgaaa acatatggat
    11401 ttcctcctta ttcaaatgct tgggtagctt ttactgcttt tttccaaccg ttgtaaagtt
    11461 tttctcggcg ctcttcctcc atttgaactt caaatagctt gccagcttca taaaattcgc
    11521 ggatctcgtc tgtatctttc caataaccga cagctaatcc tgctaaaaat gctgccccta
    11581 gcgccgttgt ttccaagttt tctgctcgtt ggatcggtac attcaagata tctgtttgga
    11641 attgcatcag atattcattg tttgctgcac cgccatctac tttcaataca ggaatcttga
    11701 ttccagtgtc atcttgcatc gtatcgatga tatctctcac ttgataagca atcgactgta
    11761 aagtagcttt aatgatatct tcttttgtcg taccacgtgt caagccaaac atagcccctc
    11821 gtgctgcctg atcccaataa ggagcaccta gcccaacgaa tgcaggaaca acataaacct
    11881 catcttcgct tgtagagcgt ttcgctgcgt cttctgaatc gctagctttt tgcaacattt
    11941 gaagtccatc tctcagccat tgaatcgaag aaccagccac aaaaatactt ccttctaaag
    12001 catagtaaac ctttccattt atgccgtaac caattgtcgt caataaatta tttttggata
    12061 attgcggttt ttcaccggta ttcatgacaa taaatgaacc tgtaccataa gtattcttga
    12121 ccatgcctgg ttcgaatgcc atttgtccga ataatgcggc ttgctggtct cctgccatcc
    12181 ctgaaattgg tgcttcgctt ccataaaaat gataaccttg cgtataacca taaacttctg
    12241 agttagaagt gacttttggt aacatcactc gaggaatatt tagtaaatct aaaatctcct
    12301 gatcccaatc tagctgatgg atattgaaca acatcgtccg actggcattc gagtaatcag
    12361 taacatgtgc ttgtccatcg gtcagtttcc atactaacca agaatcgatc gttccaaaca
    12421 tcaattcgcc tttttcagct cgttcttgcg cgccttctac atgatcaaga atccagcgta
    12481 tcttcgttgc tgagaaatag gcatcgatca ctaaccctgt tttttcgtgg aaaaattctt
    12541 gatagccttc attttttaag ttatctgcaa ttccagctga ctgtctggat tgccaaacaa
    12601 ctgcatgata gattggacgt cctgtatctt tttcccatat tactgttgtt tcacgctggt
    12661 tcgttatgcc aatcccagca atctgatttg gtttgatccc tgattcgatg aacgctccgg
    12721 caataactga ctgtacagaa ttccagattt catttgcatc atgttccacc caactttctt
    12781 gcgggaaata ttgcgtgaac tctttttgag aactgccgat gtgtcttcct ttcttgtcaa
    12841 agataattgc tcgtgagcta gttgtacctt ggtcaatcgc catgatatac gttgattctg
    12901 tcattgtgat tccctccatt atatgcaaac gctttcttta actactctta tcatactact
    12961 gttttaaaaa aagaaactgt caaaatttta ttcttttttt ctttttttga cagtttttta
    13021 ttatgaagct attttttaat caattctttt cttttttcag cgtctctatt cttcttttca
    13081 atcgaataat gtcgtaaggt gacgcaaatt ctgacagata atattcacac tgttttttgt
    13141 ttctctggca aaatgtgatc accaaatcat attctttacc ttcttcataa cttccaatct
    13201 caataccgcc gattcccctc agttctctga tcagatattg ttgaaaagca atacgataaa
    13261 tcggcagact ttctaaatcg attccaatca ctactggttt tgcaatataa aaatcgatca
    13321 tcaaaagaac attggcatac tggatcatca ggtaactaag ctcactatct tcagctcttt
    13381 tcctcggcaa ttgctgcacc gctgtttcct gtaatttcgc cagtaactgt gttaaggaat
    13441 gcccgagcat tttctgctgc tgttccaaaa gccgatcacg ctcatagact tcgatctttc
    13501 caacaaagaa aaaccattca ttgttgatct gggaaatttg gtagcctact gccttttctt
    13561 ctttgatttt caaacgacga ggacgataat gccataacat cgtttcccga atgtaagtat
    13621 ctaaaacagc ggttggtaac tttttgctcc gtgtcaaatc atactgataa aatatttctt
    13681 cgtctactat cgaaaaagaa acaaaaaagc tgtaaaacat cattgtttcg tatacattca
    13741 gttctgctgc tgttcgacta agatgcatca cgatgattgg atcgattgct tgataaagcc
    13801 ggtccgactg atagagtaat ttcttttctt tcaaagttgc gtacgtactt ttttcattca
    13861 atagtctttt tttcgtaatc ccgagccagc aagcaatctg tgcttctgct gatgccgtaa
    13921 acgttgtttc taatacacgg tccaatccta gaatgaacgg tcttttttct ggtgtatttt
    13981 gcaaaaaaag cggtcgctga tcttccggga tatattggaa tagttcataa taaaaatatc
    14041 gtatctgtag ctcttctccg tataattgac cgtttttgat ttgtaatcca aattcctcta
    14101 agagctggtt caattctttg atctttcgaa aaacagaaga ctcactgata ttaaacgctg
    14161 tcgctaatcg aacgatggat acttgctgat gttctaataa atagacgagc atcttaaact
    14221 tcaagctttc ctgcaataaa aacaatagaa ttttttccaa acttacatct tcgtccaaca
    14281 caagcagcgc ctgatactca tttcgctgta cttctacttt tttttccatc attcggccaa
    14341 tcaattcaat atcttccaaa tattgatcaa acgaggcttt tgacagttgg acatgctctc
    14401 tcaaaccatt gatagaggct gttccccctt gcagaacaag gtttcgcaaa atcaggatct
    14461 gtaactgctc ttttctttct aataatgctt caatcttcat aggcagtatt cgccaccttt
    14521 gaccacacca tatctggcac acggtattct ggctggaaaa atagctcatg ttcttctaac
    14581 tgttcaaacg tccatccttc ttttactgca attgtcagtt ggtcaagaag gtggaacatc
    14641 tcttttttgc tttggagctg tgctccaaga atcttttttt cagaatcaaa tagaaatttg
    14701 agtttaactt tgctttgatt tattgctgta agcttgtagt ctacgtatcc tgtttcaatt
    14761 ttttggggat aaaaaatgct ctcttcttct gtcaaaccta cgcttcctaa gaaatagcca
    14821 aaccactgat tgccgactgg acgataggtt ccctgatcct ttgtctgtga gccactaatt
    14881 gtcttgctga catttcgtgc tgtacggaca gcgttattta ctagagaaac atacattttt
    14941 tcatttgttg gacgaaaagt tacttggatc gcatcgccaa tcgcataaac gtctttttga
    15001 gacgtttgca agtagtcatc tacccagatc gttccatcat cattcaatgt taattgttct
    15061 tcccacatct gattgtcggg gcgagaatga ttagccagca aaacaagatc atagttctcc
    15121 tttctttttt ccgtctctac aatagcggtt tcattttcgg tcaaagacac aacttgttct
    15181 tctaaaaaaa gtgaaagccc ttgatttttc atttccttta ctaacggttc tgtcatttca
    15241 ggatctagat aacgaaaaag aattgacttt cggctttcgt aaagatggat ttgttttttt
    15301 cttgatgcca acccttcagc tatttcgatt cctgcttgtc ctgcaccaat aattgcaatt
    15361 ttctttgcat tcatgatctt ttgctgaaga gccgttacgt tttgtatctt tttaactggg
    15421 tgaataagcg ttgattctgc agaaatattt cgaaactttt gatttgaacc agtcgctaga
    15481 atcaaacgat caaaatccaa tttttttcct cctgctaaaa cgactttttt ttctttcaat
    15541 cctattactg tcttttctgt gtaaacatgg attcgatagc ggtcagtcag ttcttctttc
    15601 gtgatccaag aaagttgttt ttcactggta acctttcctt ttaatatcaa attgaacccg
    15661 ctgggaatcc atcctaactt cgattgcttt tcaatcaaat atatttcact tgttggcatc
    15721 aattggcgaa gataaatagc cgcatgaatc cctgcaaacg acccgccgac aattacaatg
    15781 ttcatctgca ttcctctttc ttataaggtg ttctactgct aacttctcaa tcagaaaaca
    15841 cagtattcat aactcgcttt caaccttatg atacatagcg atgcagcaga atcaaccctc
    15901 tccatcatac cgtgttctag gtctgcttct caataatgta ctagtggacc taattgaaga
    15961 gtatcatttc tgaagagatg tttcaaaaaa tgtactctta taaaagaaaa aaactcgcct
    16021 tttacgtacc tctgtccaaa taggaaacag aaaagaacga aaagacgagt tttagcgtgt
    16081 gtaatgtaat agttagaata gacacatcat ttatttttaa ttctttaatg cttcaacaac
    16141 tgcttcgcct aattgatgag cagattgagg attttgccct gtaagaagcc gcccatcaat
    16201 ttgaaccact tctgaaaaag ctgctccaga ctgatagttt gctccttttt ctttcaaagc
    16261 atcttctgct aagaatggta cttcattcgt tgtaccattt gcttcctctt cttcattact
    16321 gaacccagtc actgtacgtc cagctaaaat agattttcca gactcatctg taactggcaa
    16381 caatcctaca acaccgtgac aaacagctga gaccagccct tcatttcgat agatttgtga
    16441 agcgactttc ccaagatcta ttgcttctgg aaaatcccac attgttccgt ggccaccagc
    16501 ataataaacc gctgcataat catttgcatc cacatcatca ggacgtaagg tatttcccaa
    16561 ggcccgattt ctgaaatctt catcttcata ataggcccaa tcgatttcat ccatattcaa
    16621 aacactttgc ggttctaaag gtacatatcc gcctttagga ctgacaaaat ctgcatcgat
    16681 cccatttttt actaatacat cataaaaatg agtcagttca cttaaccaca aacctgtagg
    16741 acgctcgatc ttttcgtact tttctacatt tgttaatacg atcaatactt tcattttccc
    16801 atcctctttc tataaaaatt gcgttattga taacgtgctt tcaagaaatt atataatggg
    16861 cttggcaaat aaagctcttt atatgcttct tcaaaaggtt tcgttacaaa ttcatcctca
    16921 cggttttcca ataatttctc tgcccagtca ggatcgacga tcatttgctg tccaattgct
    16981 gcaagttctg catatttcaa tacctgctct acatcttcgc gattgcggat cccaccgaca
    17041 ccaactaaag ggatctttcc ttgaatcgtg ttatggacca cttcaagaat cgatttatcg
    17101 ttgtatttat cagaacgagc tattcgatca tacatattta atgaaacatg cagataatct
    17161 agttttgact cacgtaatcg ttcaagaagc cagatcgtgt cttcaaaacg gatacctggt
    17221 gtttcatatt cttctggtga gaaacgatac cctacgatga atggtttttt agcatattgt
    17281 tcaaccgctg agaaaaccgt tgttaataat tcttcaataa aacgataacg attttccaag
    17341 ctaccgcccc attgatcctc tcgtctattc gaatgcggag agaaaaattg ttggatcaaa
    17401 tacgtatttg ctccatgcaa ctcgattcca tcaaatcctg attcaatcgc tctacgtgtg
    17461 gctttgccaa atgcttggat tgtttcaacg atttcttctt cactcattgc tcgtggtgtt
    17521 tccgcatcag gacgttttgc tgcaactgca cttgcagata caggctgttc tccagataaa
    17581 gttgctcgac ttgtcattcg tccaccgtgg aaaatctgaa taatcgcttt tgcgccttct
    17641 ccttggattg ctttcgctag ctcacttaat cgaggaatca tttcatcatg agcgatgctg
    17701 agttcacctg gccagccttt tccgatatct tggacatttg cagcaccagt aataacagca
    17761 ccaagacctt tagaacgttg tttataataa gtgatctcat cttcggtgat cactccattg
    17821 aaaaagctga gttgtgtcgt cataggcgac agcatcaatc ggttcttcaa ttgaactcct
    17881 gaaggtaaag taattgattg atcaaattgt gacattgtca ttcctcccat aattcgctct
    17941 gattaaccta tcgagaatca tctatcatta aaccactcga ttagttgtac atgcaactaa
    18001 tttgcttaaa aaattttatt ttttttgtaa cttatctgtt gcatcattca ttgtctgggc
    18061 taattcttta cttgcttcat ttcccaaaac tgctttgtat gactgatgaa gttcttccca
    18121 gctaccatcg acttccttag caaattggcg tccttgttct gtaagataaa ctaatgacaa
    18181 gcgaccttcg ctaactgttt tgacgtatcc tttatattct aatttttcaa caaaacgagc
    18241 aatcgttgat ggggcggaat agagcatttg tgcaatatct ttttgtggta ttccatcttt
    18301 ttcttttaat atcaataaaa taaaaccata agttggtgcc agacctgtcc ttctaaacgc
    18361 ttcatcagct aatttattca acatgcgatc tagctttttc acgctaaaga aaagacattc
    18421 atctaacgct ttttcaattg gcttttccat ttcatcccct ccattggcgt acatatcata
    18481 gtatgatttt tattttttgt caaaaaacac ggaataatgt ccgcctcctc tgtctagatt
    18541 tgaaaaaaag aagtagccat cttcaaagaa tttcggcttg ttttcgacaa aactccttct
    18601 ggaacaccgt gaatcagagg ttgtgaaaaa gtcgtttagt tttgagtatg aagaaaaatt
    18661 ttgtgaaaat ggctttccac atttttgcat aaatttggct taataccgaa aaactggctt
    18721 ttgaacaccg tttatatcag aggttgtgaa aaagccgtcc tgcatcaagt aataagaaca
    18781 gccaaacaaa aatagttcct catattttgt cgttttttga gctaatgccg atggtcagct
    18841 gattgtacac cgtttattga gaggtcgtga gcctgccgtt gaactccgag tcacaaggag
    18901 caatttcaaa aaacagcttc tcatgtttta tgactatggt gacttgtgat cgaggagttg
    18961 gcaggagaat actgtttatt cgtaaagaag gggctgtgac aagacttttg tcacagtccc
    19021 ttatttcctt ttattatagg atttcgccat ttgattggat gacttgtctg taccaatcaa
    19081 atgatttttt ctttaaccgt ttctttgtgc cattcccttc gtcatcttga tcaacataga
    19141 tatatccata acgttttgac atttgtgaag tagaagcgct gacgatatca atgcagcccc
    19201 acgacgtata accaaataaa tctactccat cttcgatagc ttccttcatc tgctcaatat
    19261 gctgcttcaa atagtcaatg cggtaatcgt cttgaacggt tccatcttct tctactttgt
    19321 ctatagctcc gagtccgttt tcagctatga acagtggttt ttgataccta tcatataatt
    19381 gattcaaagc aattctcaaa cccttaggat caatctgcca gccccattca cttgatggaa
    19441 gatacggatt tttaattcct ccagttatta agtttccttg gaccccttcc aaattttctg
    19501 gatgagcact gactgcacta gacatgtagt agctaaaaga aacaaaatca acagggtagg
    19561 ctgctaagat ttcttcatca cctacttcta tttttaagtt gacacctttt tcttcaaaca
    19621 ttcgttgcgt atacgctgga taatatcctc ttgcttgaac atcactaaag aacaaagaag
    19681 ttctttgctg ctcaattgtt ttttgaatat catccggatc acaagaatac ggaacaagaa
    19741 gctgatagct gatcatacaa ccgacttgtg aacctttgat aatttcatga caagcttttg
    19801 tagctaaagc acttgctata aactgatggt gtgcaccttg gtatttcgct tgttccaaat
    19861 ggggatggtt ttcttcaatt actcctacgc taacaaaagg gtgatgtttt acacagttga
    19921 tctcattgaa agtcatccaa tattttactt tatttttata gcgtttgaat actgtttcag
    19981 cgaaacgaac gtaaaactga ataagctttc gattaggcca tccaccatat tctgtaacca
    20041 aatataacgg catttcataa tgggaaagag aaacgactgg ctcaatccca tattttagac
    20101 attcgtcaaa cacttgatca taaaaaaata atcccgcttc attgggtgtt tcttcatcac
    20161 catgaggaaa aattcttgtc catgcgatag acatccgata gcatttaaaa cccatttcag
    20221 caaataaacg gatgtcttct ttaaagtgat gatagaaatc atttccgtgt cttttaggat
    20281 aataatactt gctgttggga tcctgcgccg cttttacttg ctgatgcgtc atcatatgcc
    20341 attgatctgt ccatttttct ttaggtaaat cgctgtcgaa tgtatacaca tcagacacag
    20401 ataatccttt ttcatctaca tcccatgcac cttcacattg attggccgct gtcgctccgc
    20461 cccataaaaa tccttctgga aattttttca tatcgatccc cctaaacttg ctagttttct
    20521 tgtttgatta tttttcttat gactagtaat gatatttttg aatctactac tatttgtaac
    20581 aataaccatt gtggtcggat catatccctc tttgattaat tcttgcaaat ctacttccat
    20641 caataaatcg cctttacgta ctttttttcc atcagatact gcactttgaa agtatttgcc
    20701 atttaaattc actgtatcaa taccgatatg gatcagtatc tctgctcctt cttcactgac
    20761 taccccaatt gcatgtttcg ttggaaaaac tgttgaaact tccccgttta caggactgta
    20821 gatttttccg tccgttggaa tgatcgcaaa cccttctcct aaagatccac tagcaaatgt
    20881 tggatcgttt acttcagata aatcaattat ttcacctgct gcaggagtac ttaacactat
    20941 atcctttgag gattcttctt cgatttcatc cttgtagccg aaaagaaaag ttagcataca
    21001 ggaactgatc atcgctaaga aataactagc aactagccaa acaaatcctt caccaatata
    21061 aatcgggaaa gacaaaatac ttcttgtagc aactgccaaa gcaccggctc ctgcggcacc
    21121 agctactcca ccaaatatag ctccacatat acaagcaatg taaaatggtc gtttatattt
    21181 aagagtcaca ccataaacag caggttcagt gattccaaat aatgctgtga taaatgcaga
    21241 taaagataaa ctttttactt tcttattttt cgttttcaaa aataccccaa gtactgctcc
    21301 tgcttgcgca gctacggcag tcatacatgc tggacctagc gtatcacgac cgtagaaaga
    21361 aagattgttg gtcattactg gtacaagtcc ccaatgtaac ccaaaaatca ctaacacttg
    21421 ccaaaatccg ccgataacag caccagataa aacagggcta aagttataaa cagctaaata
    21481 tccagctgca atcagctgac ttgcataatc tgcaattgga cctacaacta ataaagtaat
    21541 tgggaccatc acgatgatac aaatgaatgg tgtcaataaa tttcgaatac tttcgtgcaa
    21601 gcttttgcga atcttaggct caatataaga aagaacccat acagctaaaa ttgctggaat
    21661 aaccgtactt gtatatcttg ctagaatcac gggcatccct agaaatcgca aggttatgct
    21721 atcactatat gcactgacaa tcgtaggata aacgagagct gctgcaataa ccatagcgat
    21781 gaaacgatct gtattgaact tttttgcagc agtatatgcc aaaaatatag gcaagaaata
    21841 gaaaaatgca tcagctgctg cattcaaaat gatataagtt cctgattcag tagttaataa
    21901 attcaaactc gtacataagg cgagtactcc tttgatcaaa ccacttcctg ccaagacgcc
    21961 taaagtcggg gcaaaaacag cggcgatcat gtctataaaa gagtttaaaa tgcctttttt
    22021 ctcagttcct ctctttccac tagatgcatc atcttctagt ttcatttgtt tggataatac
    22081 gtcatacact tcatttactg catttcctac tacgacttga aacattcctc cagcttcgac
    22141 tacagtgatc acaccttcta gttgctctac cttctcctta tctgccttct tattatcttt
    22201 tagggtaaag cgtaaccttg ttgcacaatg gtaaacattg ctgacatttt cttcgcctcc
    22261 gacaaatcgg acaatatctt tcgctaattg tgaatagtcc atttttactt cccccttgtt
    22321 aattaatgcc gtagcaatcc ttcttacaag ataagtatag ttcttagaaa acggtttctc
    22381 cattcaatta gtaccccttc taatagcgaa cgaaactgta caatagtgtg tatggtttac
    22441 ttagccgagc aaatcactca ctgcttgaaa gaaaacaagt atatcgtttc tcttatagtc
    22501 aaaggagaca aaaaaaacac gcttgatctt cctttgctga tgtataagtg gtatctcaag
    22561 ctgatactct gtgatttttg tttctacttg gcttttgata agacagcaca acaaagtctc
    22621 ggcatttaac ggaagcataa ctaggtcaga actaggagag attagttgct gaatggaatc
    22681 tatcttttta tggattttta tttgattttc ttctttgtag taatgatcaa gcagttttgt
    22741 tttctttttt tcttcaacta tggtcagctt ttcacgcaaa atctgataaa tctccgaaga
    22801 ttcaaactca tctagaacac taggtagata aatcaaatca tcccactcaa aaataagctc
    22861 ttgctcagta gtcaaaattg catcatattc cgttattttg tttgattgat ttttgaaaag
    22921 atacactggt tctatccggc attctttgat ccacatgttc atactttgtt gaatctgttt
    22981 ttttagtgta ttcaaaatac taattggctg gttactaaca atcagtatct ccccttgttt
    23041 tcgaaaagga ttcaacgctt cggcaagata caaaaccaaa taagcgcttt cagctaaatt
    23101 gggacgtaat tgaaaatgta ttgggaagaa acgccgaatt aaataaattt ctaaaggata
    23161 acgggagatg atcttttgta caaaatgatt catggtatta tgtccggcat ccatcctcgt
    23221 tttcatttgt ttgatatggg ttagcaaaaa ttctgtttca tggaacaata acgaaacagg
    23281 aagatctaaa aaacgaataa tcgtttgctc aaatgctctt agattcttct ctatttcttt
    23341 gtttttttca tcattaatca tcaaataatt taactctaat agtcgatttt caattgcttg
    23401 tttttcttga ttggaaaaag aatagggtag ttctgatgat aacttttttg tgagggtctc
    23461 taccatcgga ataaataatg tatacctgtt tgttttttct aaaagttctt ctcttcgaag
    23521 aatggtcaaa acgagatacc gagaaaatcg tatgaagtcc tctcctgaaa caatataatt
    23581 atatgatata agaacctctt ttaatgtctt ttggacagtc ggcatgatat tggaaaaaat
    23641 tgtactgatt tgttgaggca accttgcgtg ttggatcacc gtttctgttc caacaagaga
    23701 taagaaaatt ctttttgaat tttcttcagc aatgattttt agtccttttg tcgacgaaac
    23761 ttccagttct aatttaggat tgcgctcgat ccaacgcaaa atcgttttta tttgtaatga
    23821 tacggctgtt tttgaaagaa agaaaatgtc tgctatcttt tgcatactga catacccttt
    23881 ttgaaacaat aagtacgtca caatagttga agctcggaaa tccatgctgg aatagatttt
    23941 atgtttcgtc acctcttgta tgatttgctg tttagtttca gattcaattg attctaaaaa
    24001 ataacctgaa cgtttatttg actggataat gattttcttc tcgtagatag caagcatatt
    24061 tttgatttca gtttggatcg ttttcttgtt ggtacctaaa gcttcagcta attcttcgct
    24121 tgttatccaa tgattcttat ttcttaatac tacaagaatt tggagttgtc gtttatttaa
    24181 gtccatttgc ttacacctcc tctttgttat aaacatacag gaaaaacctc tcaaatgaca
    24241 atagtcactt gcagaggttt aaaaatcatt tgaagaaaga tctatattca aggtttttac
    24301 cattagactg gataatttgt ttataatatt caaaactatc ttttttcaat ctcctaaagc
    24361 ttccgctccc atcattttgt ctatccacat aaataaagcc atatcgcttc ctcatttccc
    24421 ctgttcctgc cgatacgata tcgattggtc cccaccataa gtaaccaatg atttggcaac
    24481 catcttttat tgcctcagca atttcctgaa gatgtatttt tagatactca caacgttttg
    24541 gatcgtggat tcgattcaat tcatctggtg cttcatctag tccaatgcca ttttcaacta
    24601 tgaataatgg taattcataa cggtctgtca attcattcat gacataacga aaccctagag
    24661 gatcaattgg ccaactccaa ggttcgggag atttttcctt caaaaaggga ttatcgaaac
    24721 catttgctcc gccagtatct acgcgcattt ctgcttcttt tttatagaca gcgctacgat
    24781 aataactaaa tgcaatataa tctaccgtgt tagcttgcaa gtactgttcg tctccatcta
    24841 acattactgg tgcacagtct tcttccttcc atatcctgta aatataagct ggataatgcc
    24901 ctcggcacat gacatcggag aaaaaccatg aattgcgttt aaattgcagt gaaccaaata
    24961 cattctctgg atcacagtca tatgcataag tcgctaatgc agaaaagcta cacataatac
    25021 ccatcttagc atttggatcg attttcttta ataattgagt agcacgggcg ttcgctagaa
    25081 acatatgatg acaggcttga taaatctctt tttttgttag tgtagcaatg ggttcaagtt
    25141 tatctctagc agggaatgta accaatccag cagctgcata aggcatacga aaagcattat
    25201 tgatctcatt aaaggtcatc caatatttca ctttcccttg gtagcgttca aagacggtct
    25261 gcgcataaaa ttcaaaaaag tcgattaatt ttcgattttg ccacccccca tactctccta
    25321 ccaatgctaa gggggtttca taatgactaa gagtaattac tggctcgata tcatatcgat
    25381 ttaatgtatt gattagtctg tcataaaata cgagaccagc ctcatttggc ttctgttcgt
    25441 ctcctcttgg aaatattcgt gcccatgaga tgcttgttcg aaaagctttt aaacctaact
    25501 ccttcagtaa acgaatatcc tcttcaaatc gatgataaaa atcaatcgct tcatgagaaa
    25561 gataatggag tgattcgtct aaagcaatct cccaaatctt tttttcttca ttccactgaa
    25621 tactaggatg ctggttcatg atcccgataa gcgtatctgt cacattaggt aatttgccgt
    25681 cttccaacca tgcaccttca cattgattag cagctactgc tccgccccat aaaaaatttt
    25741 ctggaaatat ttcctgatcc ataactatct ctcctcgcta tcaacaattt cttacttgaa
    25801 gtatagctcg attcatcaaa acaagatatt ctaactattt cggcagaaga gaagaaaaaa
    25861 attgaatgct actcttcatt tttaatattt atatataaca aatacataag acgatccccg
    25921 aaagaaaaca ctcgttaacc atctaagcaa acgatttatt ttacttccaa cacaaatgct
    25981 tcataaggtc gtaaatcaac tttgccgtct ggtaactgtt tcagaggata atttgacaca
    26041 ataaccctta aagcttcacg tggaacagtg aacgtctgct tttcactgga catattacaa
    26101 ataacgagcc attgctggtc ttcatatatg cgagaatagg caaagacatg ctcaggcgtc
    26161 ttttccacta attcatagtc tccccagacg atgatgtcat gttttctacg caaatcgatc
    26221 aatttcttgt acatataaaa aacagaatct tgatctgcta acgcttcttc tgcattgatt
    26281 tctttataat ttggattgac tcgaagccat ggaattccag tagtaaaccc agcatgttca
    26341 cgggcgttcc attgcatagg cgtcctagca ttgtctcttc ctttcgcatt gattgattcc
    26401 aagatttcct cttttgtaaa tccagaagaa atcctttcgt tatacatatt gattgtctca
    26461 atatcttttg cttccgcgat atcatcgatt gggcaattcg tcatgcctaa ctcttctcct
    26521 tgatagatat aaggggtgcc tttcatcata tggagcaaga tagcaaacat cttggcactt
    26581 tcttttcgat acacatcgtc atttccccaa cgagaaacaa ttcgcggaag atcgtgatta
    26641 ttccaaaata aactattcca tccttcatca cctaaagatg tttgccattt ggataatact
    26701 tttttcagtt cagagatact caagggtttc aaatcccatt tgtctttgcc ttcttgctga
    26761 tctaatccta tgtgttcgaa ttggaagacc atggataatt catttctttt aggattggaa
    26821 taaagtttag cgatttcggt agttgctccc catgtttcgc caacagtcat cagatttttg
    26881 tcaccaaaag ttgcttgatt catttcctga agatactcat gcagtttagg tccattactt
    26941 gtgatttctt tatccggttc tttgccaatc atatcgatca cgtccatacg aaagccacca
    27001 atacctttat cgatccagaa attcatcatt tgataaatct cttggcgaac ctgtttattt
    27061 ttccaattca aatcaggttg acgcttgcta tacaagtgca gaaaatattg ccccgttttt
    27121 tcatccaact gccaagcaga accactgaat attgagcgca atctattagg ctcttttcca
    27181 ttgaccggat cacgccaaat ataatagtcc cgataaggat tttctttatt tttacgggcc
    27241 tcaatgaacc acggatgttc atcagaagta tgattcacca ccagatccat gatgatacgg
    27301 atatttcttt tctttgcttc ttcaatcagt tcttccattt cttccatcgt accgaattct
    27361 gccatgattg cttgataatt actgatatca tacccattgt catcattcgg agactcgtat
    27421 actggagaaa gccaaattgc atcaatacct aaatacgcca gataatccaa cctggaaata
    27481 atcccccgca aatctccaat accatcttga tttgaatctt gaaaacttct tggatagatt
    27541 tgataaacaa ctgaatggtg ccaccatttt tgttccatga ttttcctcct ataaatggat
    27601 gtttctattt tttgcttaat catttatttt agtctctctc actaaaaatt gtaacgcttt
    27661 caaatagcta ttgcaccttg ttatcggtaa cagaataaaa caaaaaaaca gctgaggaaa
    27721 ttccctcaac tgttttttga aaggattgat ttattcaggg aagaatgctg atttcacaac
    27781 ttctttataa acggtgttca ctcagctgtc cctcggtatt agctcaaaaa acggcaaaat
    27841 ataagaaact atttttttcg tttttttctc taatactcag gccagaacac tgatttcaca
    27901 acctctttat aaacggtgtt cactcagctg tcccttggta ttagctcaaa aaaaggcaaa
    27961 atatgaggaa ctattttttc gtttttttct ctaatactca ggccagaaca ctgatttcac
    28021 aacctcttta tttcacattc aatatccaca tgaatcccat gaatacaaag aacagaccat
    28081 acatgatggg agatacttct tttcctcttt tagctgctaa catggtaatt ggatagaaga
    28141 taaaacctaa tgcaatccca tctgaaatac tataagtcaa aggcatgccg atcaagatca
    28201 agaatgaagg gatcgcaatc tctaagtttc cccattcgat ttgtcgcaaa gattgtgcca
    28261 tcaatactcc taccataatc aatgctggtg cagttacttg tgaagtaaca actgatagta
    28321 atggagaaaa gaacaagcct aacaagaaga aaatccctgt agtaatagct gtgatacctg
    28381 aacgtcctcc aacggcgata ccagctgatg attctacaaa tgcaccaaca ggagaagtac
    28441 ctaataagga acccgcaagc atagctgttg aatcagaagc taaagctttc cctacacgag
    28501 gcatcttgtt gtctttcatg aaacctgctt ggtttgctaa tccaactaaa gttcctgctg
    28561 tatcaaagaa agtcacaagc aagaatgtca gcactacgac ccacatttga agagaattga
    28621 tgtcccctac atgttttaat gcaactaaaa atgttggttt caaacttggt gctgcggcaa
    28681 caatttcaga aggcattgga atcaaacctg tgaccagacc taaaatcgtt gtagctgcca
    28741 tcccgatgaa gatgccacct ggtacacgcc gaacaagcaa gatagcagtt acgactaatc
    28801 caaaaatcgt caaccaagta gaaccaacgt tgagtgatcc taatcctact aatgtagatt
    28861 cattcgaaac gatgatccca ccttcgctca gaccaaggaa agcaataaat agaccgatac
    28921 cgccagaaat cgcatatttc aaatctgctg ggatcgcatc aatgatcatt tcccgcagct
    28981 tgaagattgt gatcaaaata aaaatcaaag aagcaacgaa tacgccggct aatgctgttt
    29041 cccatggaat ccccattcct aaacaaacag aataagcaaa aaaagcattg atcccaagag
    29101 caggtgccgt tccaattgga taacgggcta atacccccat taaaatacag ccgatcgcac
    29161 tggctaatgc agtcgcagta aacactgctc cttcatccat tccagaagca cctaatacag
    29221 ttggatttac aaataagata taagccattg agataaatgt tgtaaatcct gccagtacct
    29281 cacgttttac cgtggtgttc aatttttcta tttcgaagta ggaaagtatt ttttccttca
    29341 tcttccatcc ccctaaatcc gaacattata aataaagtcc gttaaaattt cgatttatta
    29401 ataaaacaac gtgttttatc atatactaaa acaaaacata agtaaacaaa aaattcggat
    29461 tttaagcaaa atccgaacgt tccatctgat taaatccctt tatctcaaag ttcatgaaca
    29521 ttcactttgg attacaagaa tttctttatt gtgttaataa agtttcttca ttctagtctc
    29581 aaaaatcaat agtgtgattt gattttacca tttcgtttct gttcttcttt gatgaattct
    29641 tttgtatcga caggattcag attatttagt ccgccaaaac ctgtatcgac catttttccc
    29701 cagccttttg tcttcttttt tcctgatttg agattctttg ctcgaagctt ctcactatgt
    29761 tttgctcgcc gatacttcaa ataaccttct aatcttccca ttagatccac tgtttctcct
    29821 cctttttatt caatcagacg actcagcttt tcctataaca aaataattgc cttcagggtc
    29881 cgaaaaattg aaagtcaaga cgccattgtt gtctgtgatt tctcctggtt cttccatgtt
    29941 ctcgtagata gtttcaaatt catccgtaaa aaatactaaa gaaggcggtg gtcctaatac
    30001 ctctggtgaa aacttctgca caaatacttt tgggaatacg cctaattcta attcttttga
    30061 gatgctgagt acaatactct taaattcttc tggcaattcg attgtttcag aaatttctgc
    30121 accaattttt tctacaaaga atcggcaaat catctctacg tcattgacat aaagcatcac
    30181 acgtgttttt tgtatcatcg tttgaccctt ctttctattt gattttcttt cctgtctata
    30241 aactgattcc attatatcac gaaagcgcaa acaccttgtg tttgatacta ttcaagaaaa
    30301 ataaagcaat agtttctagg attgctttcc tccacaaact ttttttacaa aaaaaaaacg
    30361 gcaatgaatg ccgctcttct tatttcgatt cttttataat tctgcttctg attcatcttt
    30421 ggttagtgtg tgtctaacac tagttacgcc aaatgtttga tcgatttttt ccttcaacgc
    30481 ttcgattgcc tgtgcgccac gtgccgcttg ttcagcattg atggattcaa tgaccagcac
    30541 cgctgcttgt gtttcttccg tcagcatcgt tctttgggct tcacgccagt tgagacagcg
    30601 acagatcgct ccttgttcat catagtagat gatttcttcc ggaagtgccg gagcatcgga
    30661 atctgcacct aaaggcagga atgattcgtc accttgggct tttcctaaat gcagatttcc
    30721 tgcgatcttt tcgatatctt ctccgccgca aggtacgcca aaagacaagg aaatactatt
    30781 atagatatct acaagcggat tgattgggaa aaattcccgt tcttgatttg cacgtttcag
    30841 taaagcttca atagaagaac gtgcaccttt tttagtcttg aatgtggtaa atgcctgacg
    30901 ccactgagca atgacttcgt tttggctgaa tgtttcttct gtaaggtaat ttcttgcttc
    30961 ttttgctgcc tcattcaata attgatgata cgtttcttca ctttcttttg ttacgtggtt
    31021 atcgatacca tttacggata agatttcgat ggttgcttca ggaaatactt cccagaaatt
    31081 ggtatccact attacttttt tcatctgttc acacctcttg attagaataa ttgcttgatg
    31141 aatagatagt ctgtttgggg atcatctccc atataaaaaa catgttcgct gtatttgata
    31201 aatccttttt tacgataaaa agcgagcgca acatcgttgt gttcccaaac accgagccaa
    31261 atggctgttt tttgcttttc ttttgctttt tcaatagctg tatctagtaa aaatgtaccg
    31321 ataccttgat gtttaaattt ttttcgtaca tagattcgct cgatttctaa aaaatgatca
    31381 gccatttcct cagattgact cgttcctgta tttagtttca ggtaacccgc aggctcttga
    31441 tcgatactca ctaggaaaaa atctgtttct ttatctttta gctcttctgt aagtttttct
    31501 agtgcataag cttgtgtcaa gtaatcagct aaatgctccg gtaattatct tttccaaatg
    31561 tatctgtaaa tgtttcgata ctaatttgtt gtagtaagtc tacatctgaa ggcgtcattt
    31621 ttcgaatttc gtatttattc acggcttctt cctttcaata gatcctttta ttgccttttt
    31681 tgacaaaatt ccaatcttcg ctaacattct tgcatacttt atttaaaaga tattccaatt
    31741 gttttgcttc ttcgtcagaa agaccattta atgcgactgc attggagtag ttgttttctc
    31801 gaatgatgaa aggatagatt tctgctcctt ttttagtcgg atagagtttc ttgatttttt
    31861 tattttcttt atcttccaaa cgttcgatca tcccattgga ttctaatttc ttgattgctc
    31921 gggcagtcgt tgtacgatct actttgatca tttctgctag tttttcttgg atgattccag
    31981 gattttcgca aatacgtact aaataaagat actgtcctct tgttaaatcg acttctttga
    32041 attcgatatt gctaatagag tctagcgctc gggcaatcat tccgatgtct cgaaggattt
    32101 cagccatttc atcccacctt tattttgttt gtaagactag tttacctctt tttgttgcaa
    32161 atgcaactat ttttaataaa aaaatcaagt tcttttttaa taactagtaa agtatattct
    32221 tttcttcttt tacgaaaaag ctcaatcaca atagatctgg tttttgtttt ctaaaataag
    32281 taactgtttc ttcgttgttc gcttttctga tagctttggc aactcttcta tactaaaaaa
    32341 tcccatgttg gaagtttcat tattctcaat aaacttggcc tcttcaccgt ggatcgcaca
    32401 agcaaaaaca agtttataat actgaaaaag ctgtggaata tctttttgtt tattcgtatc
    32461 aaaaactgct cgcaactgag tagtttctac tgttaatcct gtttcttcat agacttcttt
    32521 acgcacattt tcttcaggag acaatcctat ttcagcaaat ccaccaggaa gtgaccattc
    32581 tttcgtccgt ttatcttcga ttagtaggac cttgccatct tttttgatat aggcacgaac
    32641 atctactttt ggtgttggat gtccttctcc aatcgttgtg agcgctttta tttcttcgaa
    32701 agtttctttt cctatttcac tgatcagttc caatgaaatc gtccggatct cctcaaaacg
    32761 ctctttatca aatacatctt ttccataata tagaccagct tgagaaagtg cgagtagtct
    32821 tttgtattga gccaacttct tgttcattct cttcccttct tttttagcaa atgtatgact
    32881 atgtaatcaa gtatagaaga ctggtttaat tacggcaaat aaatataatg aaatctgcaa
    32941 taaataaaag aatgttaaaa aactcgagaa caaaagacat tcgacttctc tggggctgtg
    33001 aataaatggc gttcaaaagc ttgcatctgc ggtaataagc cccatcaaac gaaaaatatg
    33061 acgagctatt cttgtctggc tgttcttatt aattgaggca ggacagcttt ttcacaacct
    33121 ccccactaaa cggtgttctc ctaccaactc ctcggtcaca agtcacaata ttcatcaaac
    33181 atgagaagct gtttctttga aattgctcct tgtgactcgg agctgaacga caggctcacg
    33241 acctctcggt aaacggtgtt caaaagccaa tttttcgata ttaagccatt tctttgttac
    33301 ttattgtcat tgatttcttt ttctaattta tccaatagtg gagtgtaatc cacttgagta
    33361 tagtctgtta catcaaattc atatagtgtt cctaactgga atttgtcata ttttcggtcg
    33421 gcgatgatct ttttgaatgc ttctttcgta atatgattgg tccctttcgt tcgatcttcc
    33481 aatacgtcac catagtgata atgatccatg atatagctca aatgacgatc tgattcacga
    33541 tcacgtttgt accgacgttg ccataacact tcaaagtctg ctgttaggcg aatcgtgatc
    33601 acctgatatc cgtatctatc tgctaattct tggagtcgtg ctttttgctt atcactaaaa
    33661 ggatactcgg acacaatcac tttctttcct gcttccatat acaaatcaag tgctttgtaa
    33721 tagaaaggat aaactttcgt cctttcttgt tccgcccgtt cttccaaatt gttaaacccg
    33781 atggattctg catagtattc cttgatttca tcgagcgtga tggcatacat atctggaaat
    33841 cgttcccgta gtttattcat aagatatgtt tttcctgtac caggactacc agccagaaga
    33901 atgaaatact ttttcatctc atttatttcc taagttaatt ggactttcca agttcataaa
    33961 cagtccaatc ttatgcttga caatttcttt tactgcttta tttgctggaa tcacattatg
    34021 gcgaagagac ttgttgattt ctgtttcact aaaggtttct ttagctactt tcgtccagtt
    34081 gactaaaagc tccgttccaa cattgaattt gcggatcccg ttattgatca attcaggata
    34141 atcttcttct ttcacacctg ttcctccatg gataactagg gggacatcaa ctactgcatc
    34201 gatttctttc agcaagggaa tgtttacttc tgtttttgat ttgaattgtc catgattcgt
    34261 accgatagca atagctaatg catccacgcc tgtagcttct acaaatttca ctgcgtcttc
    34321 cggtcttgta tataccttgt cattttcagc gacatggatt ccttcttctg taccgccgat
    34381 tgttccaagt tctccttcta cggaaacacc gtgcgcatgg gcatattcta ctactgcttt
    34441 tgtcttcaag atattttctt tgaaaggtag atgggacccg tcatacatga cagaagtata
    34501 tccgctatcg attgcttctt tgatatcatc aaagtctcgg gcgtggtcta agtgtaagac
    34561 tgcatcaacc atttcttctt ctgcaatagc tgtcattaca ttgactaaca catgcatgcc
    34621 gatatattta gcggtatcga cacttgtttg aataatgatt ggcgaaccca tttcttttgc
    34681 tgcgcctagc atggcaggaa gcatttctag gttatgcgta ttaaaagctc caactgtaaa
    34741 attcaactct tctgctgttt ttgttacttc tttcaatgtt gtatacatct tttattgcca
    34801 ccttatcttt ttatttgttt cctttgatcg ttacttgttt tgcaatttgt cgcatctcat
    34861 ccattttgcc catgtagtaa tcgatccctt gatcatcacc agcacgagca gcatctgcac
    34921 gcttctggtt atagaggctc atcagttttt tatacccatc tccgatcgtc tgtttatttt
    34981 ccaagctctt ttctaaatat tggatggctt gatcgttctc atgcaattca gaaaatttaa
    35041 gtccaacttg ttcataaagt tccgctaatt tatgcctatc ttgggtagtg gaaatctctt
    35101 gtttcaattg tgtaatctct gattgaatct tttcggtcgc ttctggcgat agggcttttt
    35161 cttcttttgc cagaactttt ttcggttctt tttctttttt cttaaaaaat ccaaacatca
    35221 ttttccctac tttcaattaa aacgctttca aaactgggct taagatccag gcatataaga
    35281 gtgctgcggc tactgtatct acatcggttg ctgtagcatt gatgaatccc atcgaattaa
    35341 agatcgttac tagcaaagcg ggcaataatg taataaagaa accatgagca ataccgccaa
    35401 tgatcgctcc tcggcgtccg ccaactgcgt tgccaaatat ccctgctgtc cctccggcaa
    35461 agaaattggt tagcatacca ggtaaaatca tcgctaagcc gaacatgggc aaagtaaaca
    35521 tggcaataat cgtaccaatc gttgtcgtaa tgaaacctaa aatgacggca tttgggctat
    35581 aaggaaagaa aactggacaa tctaaggcag ggatcgcatc aggaactaat ttcatagcta
    35641 tccctcgaaa tgccggcacg atttcaccta acagtagacg tacaccagct agcaatatat
    35701 acacacccac tacgaattgg atggcttgta aaaaggcaaa catgatatag ttttgatctc
    35761 ctgataattc agaagcgaat ggttcgcctg caaataaaac agtgatgata tatagcggga
    35821 ccatcacgac catgacagac aaataagtat cttgcagaaa ttcgaaatgt gtcggcaact
    35881 tgatatcttc gatcgacttt ttattttcgc ctttctcacc aaaaagctta gcaacaccgg
    35941 cttcaaataa gtacccgatc gtacaaaaat gacctaaagc aatatcattt gatccagtga
    36001 ttttacgaat gatcggttgc gctactgccg gcatagcaat ggcgaaacaa gctccgataa
    36061 aaccaccgac caagatcaat acgatccctc gcagaccggc aaaatagcca aagactgtcg
    36121 tcatcgttgc catccataaa attgcttgac cagttaaaaa aatgtacttc cattttgtga
    36181 aacgagccaa aataatattg aatacaaaaa tggctaagaa cgtcagtgca atatctctac
    36241 ctaaacccaa atcattcatt gcttgatcat tgatagattc aatagaaggg atgatccctt
    36301 gcatatgaaa tccttcagta aagattttcc cgaaataagt taaacttcca acgatgatgc
    36361 tcgaaccggc actcaatact tgaaatccta ggagtgtttt taacgtgcca gagatgactt
    36421 gtcctgctga tttcttctgc agtagtagac ccaacatagc aatcaatgca atggtgatcg
    36481 atgcctgagt caaaatattt tcgatgataa aattgataac gctcatgttt ttaccctctt
    36541 ttctttttgt atttagataa ctcccttatc ttgcagaact ggggtgattt tttcttcaat
    36601 ttcatttttt gaaacaatat ttttcaaata gatcatcgcc gttttaggat caactgcgaa
    36661 cttttcgaac tgcgaacgaa aattttctgc tgtgatgatc acatctggtt tcatggaaac
    36721 tgcggaagaa atatcacaat ggtccagttt ggcttctact cctaatttgt tcaaaacatc
    36781 ctcagcagac atctgtgctg caaaactact tcctaaacct gcgccgcata cgaataaaat
    36841 gttcaatttg ttcatctttg ttctcccccg tatcatttat tatttctcaa ctactgaaaa
    36901 aagcagttgt ttgaaatctt cgatatttgt gcattcaatc aatcgatcca acttctcttc
    36961 gttgttgatc aattcaatta gttctttcat gatgttcaga tgggagtagg agtctactgc
    37021 tgccaaacaa aaaataatct ttactggatc catttcagga ttaccgaaat cgattggttg
    37081 ctcaatcgta gctacgctga ctcctaattt attgacccca tcttctggtc gggcatgggc
    37141 tagtgccatg tgtttgccaa tcacgatata cggtccgtaa tcattcactg cacgaatcat
    37201 cgcttcgaca tagcgctctt cgacaatgtt ttctttaacc aatggtctag cagctagttc
    37261 aatcgattcc tcccaagtag tggctttttc tttgatcatg atattgctgt ccgtcaaaat
    37321 atcttttaac ataggctgga tctccctttt gttgatttct aaattgtttg tttcgaataa
    37381 tttttctaac ttgccataga tgattcgatc tactcggcca ccactttctt tcatgacatc
    37441 tattatttga gtgaataact ccgtcgattc atgactgtta ggctctaacc gctgtagttc
    37501 ttcatttctt ttcaaaaagt cttctataac caagatattc gtctcagtta aaatcggatc
    37561 gacaactaaa agcggcttat ttcgataatt caattgaaag gtgctgaaaa ccagattgac
    37621 atccaattta tctactaatt cgatttcttt tgaacttaaa actgcagtaa tctcgatctg
    37681 aggaaacttt tctttcagat tctctgctaa cagatttcca gtagccatcc catggttaca
    37741 gacaacgact gatttataaa cataattcaa gtctcgcttg attacactgg cgatcgtcga
    37801 aaagtggatg acaagaaaag ctatctcgtc ctccagtatc ttgccaccaa ttacttcttc
    37861 gatcgtagga acaaaatgag tgactgcgcg gtagattggg aaataatttg ttcggatgtt
    37921 ttctttcaat ggattcatga cttggatatg attcttgatt cttacaatca gtgcagccat
    37981 gtgcttatag agattttcga ataaggcttc ttcctttaga tggaaaggta cgtgtgtctc
    38041 gttctctaca aattggatca attggatcgt tagtaattga gcttgtaccc actcgattga
    38101 attggagata tctctagcat tgaacgtctc aatagtgaaa cgaagataat ttctttctac
    38161 ttgcgggaca ttgtccaatt gaaattcctc gatcatgtgg tcaataaatg cagaaaagct
    38221 actgtctttt atttctgtat ttttccaatt aatggcagag ataaattcat gtttcctgat
    38281 tcgctggatc cagatcaatg taaaaagcag gatttgtttt ttataaatat catcttcttg
    38341 tttcaacaac ttattggaat agatctcctg tatttttttg atttcctcca aaggaaaata
    38401 atggagaaag attttttgag ccggtgttaa ttgtgcatct tgattcataa agaagtcggt
    38461 cattcccaga ttctgattga taaaatcata gatcatcgta cggatagatc gttctgaacc
    38521 tttataaacc agtccttgct ttccataact gacgatctct atcccataac tgaccagtgt
    38581 tgctcgtaac cgtctcatat cttcatctaa tgtactttta gagatttgaa attcttcttc
    38641 ttttttgttt agatggatcg ggattcggct gtaagcaacg gctaaaacta gatcaaaaat
    38701 acgttcgcgc cgattcaaaa agccgtcttt cacatctttt atttcagttt ctaatcgagc
    38761 ttgcttttcc ttttccagct tcaacaaaaa acctttgttg cgaattgtac ggatctccgg
    38821 aagcttttta ctgctcaacc agatattcgc ttcaagaact tcctgacgaa tcgtccgctg
    38881 actgacattg aactcacttg ctagatgttt tgcttttact ggatattggg ctgtcatgat
    38941 tctgtgaagt atttcaattg ttcttgtttt catagaaatg atctccttct gatttcaagt
    39001 atatggttcc gcttacataa taaaaagtag atatttctgc cacttgaagt ggaaagaaag
    39061 taagcaaagc aaacaaaaaa taagccatat gctttttcat ctcctcaacg aaaaagacat
    39121 acgcctatta ttacaaaaat tttgcccatc tatacaaaaa agaggttgtg aaaaagctgt
    39181 cctgcatcaa gtaataagaa caactaaact aaaatagtgc ttcctatttt ttgtttggtt
    39241 gttcttatta tcgcagatgc aagcttttaa acaccattta gtggagaagc tgtgacagag
    39301 atgttcagcc ttgagcgata agaagcgatt ttgaaaaatc gcttcccata ttttacaaaa
    39361 tcgtgcctta ttgccgatag gctatctctg gaacaccgtt tattcagaaa aaggagctgc
    39421 gacaaggctt ttgtcacaac tccgttcata aatatttcct gtcaatccag cataatcggt
    39481 tgaataaaat cggcttgcca tcttttttct ttctcagaaa aatcaaaata ccatatctga
    39541 gaaaaatact gtcgttgtgt cttacttcct gagatcaatc gcccatcttt tcgatcgact
    39601 gaataatcac agcatctgaa atcaattcgg atggcaaaac tattttcgtg gatctgtcga
    39661 taatttccta cttcctgaat agttattttt ttagtatgat ttcttacacc gtctgctttg
    39721 tttttttgca gaactttctg atgttcggca aacagcttgt ctgtataaca gcttctggct
    39781 ttttcaggag tttcttcttc ccaagcggat tggatcttca tgaatatctt ttggatttcc
    39841 aaaaactgtg ctttcttttc tttagccgtt cctgacatgc gggacaatat ctccgatttc
    39901 agccttttct tttgtctttt attcttgacg atagaaaaag cagaaacccc tcctactgac
    39961 aaaacagcta atgcaagaaa ccccgtttgc tcataactag aaggacctgt acttggacca
    40021 taatacccat aagaatctct gccgtaagaa cgactgccaa aataactgcc cccatatgaa
    40081 cgaccgccaa atgaacctct gcccgaactt cgaccgctag atgtacctcc accatgacca
    40141 cccgcgacag cgaatccttg ggtcggcaga gcaaacaaac ataggcccac taacaaaatg
    40201 attgctaaat aattgatttt ttcattgtct tcctcgttat cttttattac aaaaaactat
    40261 ttttatcata gccttgctaa aaggaagagt caataaccag atgcataaac tgttttttta
    40321 gcaattttgt cttatttagt cattgagtaa catcggacag atgtatgtgt cggcagtcca
    40381 taattcctct atcttcagca tgaacaagct tgcagacaag agatctgcgc ttcctccagg
    40441 agagatttgg ttttcctgac aaaagtgcaa cagtgctgta aatggacgat ttggtaaagc
    40501 actttgacta gcagcatgag caagtttttg catttccact aacgttttct gatcactgcg
    40561 atgcaatacg catgtgtcat ctagcgaact catcaaaact aacaacatat gcatttgttt
    40621 ctgtacatat gaatcattat ctagctcctt agctgcttgg attgcaagcc tgatatgcgg
    40681 ataccctaat atcgcttctt catatgcacc atttacgcca tatttttgct tcaccctatg
    40741 accatgagtg gtttttttat gacgatattt tgtatcagga aaagcagcaa gcttgctgac
    40801 atcatcaagt attttcgata aagaaaaagg ctgttccata ctgatttttt gtgcaatgac
    40861 actgatcaat agccccattg cccaaatcgc tcctttatgg gtattgatgc catttgtagc
    40921 aatcagcatt gccttttccg cttttcgccc aatctcagca attctttctc ttaactcctg
    40981 atcaatagca taatgccatg ctatttgagc catttcttca aaaaaaggag ttagcgtctt
    41041 agcagactta tccaaaatgc gccgaccttc ttttacggct tttgcgatcg tatggaaatt
    41101 atcatctcct aaaaccatat cagctgcctg tttgcttact tcactacctt tgattcccat
    41161 agcgattcct atatcggctc ttttcaaagc aggtgcatca tttacgccgt ctccggtcat
    41221 tccgacgatt tctccatttt tctgcaaagc tttcacgatt cgcagtttgt gttctggtgt
    41281 cgttcgagca aagacgtcga cttttggtac ttgtcgaatc aactcttctt cagacataag
    41341 atccaaatcg atcccttcta aaactttagc agtatgtttt aagccaattt gttccgcgat
    41401 cgcttttgct gtatctttgt gatcccccgt aatcattttc actgagattc ctgcttgttg
    41461 gcattccttt acagctttga ctgcactttc ttttggcgga tcaatcaacc cagcaatccc
    41521 tgcaaaagtt aattgggtta aagactcatg cgtgagttcc tgtttcgaat ctactttttt
    41581 gtaagcaaat cctaaaacac gctgaccttt tttcgcaaac gctgtggctg tttgacccca
    41641 tgcttcttat tctgaagaag aaagagttga tttagccaat aatacttctg gcgcaccttt
    41701 gacgtaaatc acccctcctt tttcctccgc atgcatcgtt gccatatatt tgtattcaga
    41761 gctgaacgga atttttgctt ctattttttt caaaggcaga tcggctgttt cggtgtggcg
    41821 aatcagtgca agttctgtcg gattgccact taaattttct gttttctgtt cttctttcgt
    41881 ctttaattcc tgacaatttt tcataataaa cagtacgcga gtttcttcct ttgctgctac
    41941 atccacaact gtcatttcat tttttgtcaa tgtccctgtc ttatctgaac agatcactgt
    42001 catagaacct aatgtttcta cagaaggcag tcctttgatg atcgcctttt cttttgccat
    42061 ttcatgtact ccgacagata ggatcatggt caacacagct ggcaatcctt cgggtaccat
    42121 tgacaccacc aaagcgatca tagctgaaag aaggatatgc caatccatgc catagcggaa
    42181 tgttgtaaaa acaactaaga acaagatcaa acaaagaatg ccttgaaaaa tttgtttgtt
    42241 caatcggttg atcttcttga tcagaggcgt cgtttgttgt tcgaccgatt gcagtgcttg
    42301 attgatttta ccgatttctg tcgtgtttcc tgtttctaca acgacaccga ctgcagaacc
    42361 agattgtaca agcgttcctg aaaaagccat atttttttgg tcaccgattg attcttctgc
    42421 tgccaaaacg gaatgatttt tttcagatgc ttctgattct cccgtcaaaa tcgattcttc
    42481 tactagtaga tcatgtactt cgatcaaccg aacatctgct ggaacgacgt ctccggcacg
    42541 taagacaatg acatctcctt ttactagttc tttcgcaggg attttttctt gcatcccgtt
    42601 cgatagtact atcgcttctt gtcccatcag ctgcttcaaa ccattcaacg attcttctgc
    42661 ttttctttct tgccaataac taacgagtcc gttgacgatc actacgaata aaataatact
    42721 tccttcgatg tattcgctcg aagcaaattt caaaaaagag gcaatgatca acacgatcat
    42781 cagcagatca gtaaagtgtt ttgctatttt tttccatcgt tttgtctgct gtcttgcttc
    42841 tatctcatta tatccttgat cagtcaggcg tttctgccgt tcttcttgcg acaagccttc
    42901 tgttgatgtt tgatagtttt tcaatacatc ttctaccgtc aatctgtacc agttcatcct
    42961 atcccctcct gtttaattga tactgttatt atacgcaggg aagttttttc aggcgtatcg
    43021 attatttcag gggggtctga aataattcgt tttgcagaat gtatctgggc ctaactgata
    43081 tttctattgc ttattctttt atatacaaaa tttaagaata caaaccagat aaccaataaa
    43141 aaaggaataa agcgtgtcgt gctttcgaat aacaagctga aagcaattga agtaaaaaac
    43201 accaacaaag caagatcttt atatctttgt gtagtgttcc gtttcttttc tttaataaat
    43261 tgaacatgtg cacacaagat cgaagaccac acaaatagaa aacaactcgt tgtcatgcct
    43321 gccaaaagct gaaaaaacaa tcgactatca gctacaaaaa aatgtagaca aagaccgaca
    43381 aaaaataaca tccctgtaat taaaataccg tgtaaaggta cgcctttctt agaaagcgca
    43441 caaaaccaat ggggcaaact tttctctttt gctgtttgat ataaaatcct gctagtactg
    43501 tagactgcac tatttgctga ggacaaagaa gcaaccaaaa tcattagatt gacaattgct
    43561 gctccgcttt ggcaatggat cacttgcagc atagtcacta aaggactttc ttttaaatct
    43621 aaacaatgcc atggaacaac aaataaaatc gacaaaatcg ttcctatata gaaaaagagg
    43681 atacgccaag gcagacagtt gattgcattt ttcaaattct tttgtgggtc actcgtctca
    43741 ccaactgtta atccgatcat ttcaattcca gcaaaactga ataaaaccat aggaaatgaa
    43801 gcaatcattc cagcaaagcc aaacgggact aattcattga gctgtccaaa atttgctttg
    43861 attccaatag actgggtatg aaaatctgag acactcaatc ctatacccaa taagataaaa
    43921 aggaaaatcc ccaagatctt gatgattgag aaaaaagatt ccaattcacc aaaccacttg
    43981 actgcaacaa ggttgactgc cattaagatc attagtgcgc tacctccgct tatcaaagct
    44041 ggtacatgta caaaccagaa atggatatac aatcctattg ctgttaattc cgcaagtcca
    44101 gctccaagcc agcaaagcca ataagtccaa ttgacaaaaa atgcccactt tttgcctaaa
    44161 taatagtcaa cgaaatcact gatagagtta aatgtactat ttgctaaaaa taaatcaccc
    44221 atgacccgca tcaaaccata aatagccaaa ccgcatatca aataaacgag aataacagct
    44281 ggccctgctg atgcaattgc ctttgctgaa cctaaaaata atcctgttcc aattgcgcca
    44341 cctaatgcga ttaactggat atttcgatta gttagagtcc gtttcattgc cggtttttcc
    44401 atcaagttca cctttttctt tttcaagcag catggcgact tcctgttccc aagaaggttt
    44461 ctttattttt tcaatacaga gtggtactac aaacaaaatg acactgatgg ctgtgactgc
    44521 aattagattg atccatgtcc cagctttcat cgtcagtccg acagaaaatc cgattgccgc
    44581 aaataaaaca ataacgacag tccaaactcc aagattgctc tttttctctt tttttccaat
    44641 tctaaatggt cgattcaact ttgcatgtgt ataacgcaat ctcaaaactg cgatccccat
    44701 gatgacatag acgatacagt agataacagt agttgaattt acgagcatca aaaatgcttg
    44761 attgacgcca ggaatcagca aataaaaaag tgcaaagatt gagataatga cagactgagt
    44821 caaaataacg gatttggata cgtcaaattg attcgtcttc caaaaattga attttggtgg
    44881 atacgcacct tttctagcac tttctgtcac tgtctttgct ggtcctgagg cccaagcaga
    44941 taattggact gctacaccaa tgaagacaag taaactaaac agattcacaa taagatgtgg
    45001 caaaccaaga atttggcaat aaatcgaaat agattgcgcg atattattca attccatttg
    45061 tccttttggt acgacatttg ctacaactaa agcattcaaa atattgacga aaaatataaa
    45121 aattaacgcg gcaaatactc cgcgaatata cgttttaact ggatttttca accgagtgat
    45181 atacacagag gacatttcga taccagtaaa aataaacatg atcggcgcaa aataaacaaa
    45241 tgaggatgca gtcgtagtat ctggtattaa tttgtcccat gaaaaactgc ctaatgttcc
    45301 ggttggtacg atcccaactt tgatccacgc agcaaaccct aataagagca tcatcgtcaa
    45361 aggcacgtac aatcctaacc aaacaccgac ttttccgccg atttttgcca tatcaaattt
    45421 caaattcaaa aaagtgatga tccaataaac gactaaaatg acaactaatg taaatttgct
    45481 gttcaaacca agcggcacat tatcgataat attaccaaac aacggagcaa gtgctgaagc
    45541 gaccatcacc atcccaggaa acatttggac ccataacagc catgagacaa cgaaccccca
    45601 tttttttcct agagcattcg atacccaaag ttctggacca cctttttggg gaaacattcc
    45661 ggcaaattca ccagagatca atgagattgg aaaagcgtac aataatactg caaccagcat
    45721 gtaaaaaatc atcgtccagc tcgttgctgc tacatctgga atatttcgta cacttgctac
    45781 aagggcgcag gtcattccga taaaggtcat ggtcgtgatt tgctttgatg aaccagtatc
    45841 tttcatcttt attccttctt tcaatagcga catatgtttt tgttttcttt tatttttgtt
    45901 caagaacaga aatgacaagg tttcatcgat tcagtacaaa acccagtatc accgaaacat
    45961 ccattgaacg atcttagtga actgggtttg atacatgaaa cgaacgatcc aacttataga
    46021 acgttttata aaaaagaata tcttggcgag gaatatatga aaaacagggg gaaatacaat
    46081 tgttttgatg aagcaaataa ttgtctgttc tccatatgtc tcttgttatt ctttcacaca
    46141 aaaatcagtt ctattggatg atttcgttta gtttcttttc cattgctttg gcgatttctt
    46201 ttgtataaaa attgaaaatt cgatcatcat tcaaataagg agtcatgata gccgcacgta
    46261 gcagagtgat tttctgttct tttttccatt cttctttcgt tagacccatt ctttcaacaa
    46321 aacgaatcgg agaatcaccg taatcttctt gtgtaaatgt agtatgagaa gtaataaacc
    46381 gttcaccata aacatcgcca tccatataag aagaccgatc gaacattttt tcatttaatt
    46441 cattgatagc gttcaaatct gtacagcctt gttctttgaa tacccaattg accatattaa
    46501 aatcaggatg attcaatgga tagacttcga ttgtttttcc tttaacagta aaagtcagat
    46561 gatcgagaaa ttctctgaat ctttgagctg cctcgattga agccccaatc aattgaccat
    46621 atccggtcac attcaatggc aatacacgat gtgctgtcca gactgcggcg gcggttgctc
    46681 cagcttttga tccttccaaa atataagctc ctaacatatc tggagccttt actgattttt
    46741 caaacacata tggcgcgaaa taagaaatga tatttctcat atttttatgt ttgattacga
    46801 ttcctccagc agcataaggg acatagccca tcttatgagg atctatcgtg acagaatccg
    46861 cttctgaaat agctctgaac ccttcatata cctctttgtc aatcgttacg cagtggtgga
    46921 aaacatgatg ttcttcaaag aattcagcta aggaagcata cggcacaaat tctcctgctt
    46981 cgtttagaaa cagtgaacgc gcatagccgc catatgcggc atcaacatgt aaataaaaat
    47041 agatcccttc atcttttaac ctttctctta attggacgat tttatctaca ctgtctactt
    47101 gaccttcttc tgtcgttcct acgacagcaa ccacacctag aattgggatt ttttgttcag
    47161 ctaattcacg aattgttttt tccaaagcat taatatccat tcgataatcc tcttgaactg
    47221 ggatagcaac catctgatct aagcctactc cgcaaatatc taacgccttc atccaagaat
    47281 agtgttttgt ttgaggcacc agccatttcc ctaatctttg gatatttttt ccgctacggg
    47341 aagaagctgc tttgacctca tccatttctt cgtccgtaaa gctttcggtc atttccaaaa
    47401 tttcttcaac agataaattc aaaagttccc actcagacat ttttttcact tgttcaggca
    47461 gcacttcctt gacagctaaa ggaatcgatt tgatgcagcg ggcgtaccat aatccttcaa
    47521 gattagcaat agaaccatct gctgcaatgt gtccccatcc gtctgtcata ttgaataatg
    47581 aagcaaaatc ctgtcctact tctgcttcca tttgcgaagt tgccatagaa gattccaatg
    47641 caacattatt tgggttccac agcatcgcat agttgtaagc taataaagca ggcatcaatg
    47701 tttcagcatt catttgtccc cagtaacggc ctgctgaatg ccatgggatc gatcctgcgc
    47761 gtaatcgctg actgacttca tccaagacct ctatcatatg gtcgcgggtg gctagataat
    47821 ctggagtgag cttatctcct tcgctaattg caggcatatc tgaaggaata taattttctc
    47881 tccatccaag atgttcatct accatcttgt tcaacaacat tttataaact ggtccatttt
    47941 cagctttgtc gcctataaag acggccttga tatccatatc tttcattttt cttccctcca
    48001 tattttgttg gtcaatgtct cgcagataca tgtgtttatc tgtaacacct cttcactata
    48061 gcgccaaaaa tgagaaattt ttttttataa catcaaatcc acgtagattt ttacaacgcg
    48121 aattgtaaaa ttaagttaga aaaataaaaa ggcatttatg gtacactcaa attgtatttc
    48181 cgacgaaaga aaacaaagga gtgtaaccat aaatgaccta caaacatctt accatagacg
    48241 aactgacaat gatagaatca tattatcttc aacataataa gccggttgaa atcgctaacc
    48301 gaatgggacg tgctatacaa actatttata atgtagtcaa taagttcaag caaggcaaga
    48361 ctgctcttga ttattggcac cagtataaag aaaataagaa aaaatgtggt agaaaagtca
    48421 ttcaattacc tgctcatgaa gtagattaca ttaaag
//
LOCUS       DAZUMK010000009        46927 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000009 DAZUMK010000000
VERSION     DAZUMK010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.009"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            190..420
                     /locus_tag="ACG808_000514"
     CDS             190..420
                     /locus_tag="ACG808_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFS5046169.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            517..1818
                     /gene="murA"
                     /locus_tag="ACG808_000515"
     CDS             517..1818
                     /gene="murA"
                     /locus_tag="ACG808_000515"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFS5046170.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1831..2007
                     /locus_tag="ACG808_000516"
     CDS             1831..2007
                     /locus_tag="ACG808_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFS5046171.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2142..2438
                     /gene="yidD"
                     /locus_tag="ACG808_000517"
     CDS             2142..2438
                     /gene="yidD"
                     /locus_tag="ACG808_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFS5046172.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2623..3090
                     /locus_tag="ACG808_000518"
     CDS             2623..3090
                     /locus_tag="ACG808_000518"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5046173.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3175..4059
                     /locus_tag="ACG808_000519"
     CDS             3175..4059
                     /locus_tag="ACG808_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS5046174.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4173..4409
                     /locus_tag="ACG808_000520"
     CDS             4173..4409
                     /locus_tag="ACG808_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFS5046175.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4497..4577
                     /locus_tag="ACG808_000521"
     tRNA            4497..4577
                     /locus_tag="ACG808_000521"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4531..4533,aa:Tyr,seq:gta)
     gene            4584..4655
                     /locus_tag="ACG808_000522"
     tRNA            4584..4655
                     /locus_tag="ACG808_000522"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4616..4618,aa:Gln,seq:ttg)
     gene            4780..4950
                     /locus_tag="ACG808_000523"
     CDS             4780..4950
                     /locus_tag="ACG808_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046176.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVRVKSKRP
                     TNHTKKWMAKNR"
     gene            5170..5877
                     /locus_tag="ACG808_000524"
     CDS             5170..5877
                     /locus_tag="ACG808_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5046177.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5936..6580)
                     /locus_tag="ACG808_000525"
     CDS             complement(5936..6580)
                     /locus_tag="ACG808_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFS5046178.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6706..6993
                     /locus_tag="ACG808_000526"
     CDS             6706..6993
                     /locus_tag="ACG808_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046179.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7101..7421
                     /locus_tag="ACG808_000527"
     CDS             7101..7421
                     /locus_tag="ACG808_000527"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5046180.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7502..8815
                     /locus_tag="ACG808_000528"
     CDS             7502..8815
                     /locus_tag="ACG808_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFS5046181.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            8812..9531
                     /locus_tag="ACG808_000529"
     CDS             8812..9531
                     /locus_tag="ACG808_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFS5046182.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            9659..10210
                     /gene="raiA"
                     /locus_tag="ACG808_000530"
     CDS             9659..10210
                     /gene="raiA"
                     /locus_tag="ACG808_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFS5046183.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10432..12966
                     /gene="secA"
                     /locus_tag="ACG808_000531"
     CDS             10432..12966
                     /gene="secA"
                     /locus_tag="ACG808_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFS5046184.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            13092..14193
                     /gene="prfB"
                     /locus_tag="ACG808_000532"
     CDS             join(13092..13163,13165..14193)
                     /gene="prfB"
                     /locus_tag="ACG808_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFS5046185.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14298..14984
                     /gene="ftsE"
                     /locus_tag="ACG808_000533"
     CDS             14298..14984
                     /gene="ftsE"
                     /locus_tag="ACG808_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFS5046186.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14977..15861
                     /gene="ftsX"
                     /locus_tag="ACG808_000534"
     CDS             14977..15861
                     /gene="ftsX"
                     /locus_tag="ACG808_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFS5046187.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRHREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            16013..17461
                     /gene="cls"
                     /locus_tag="ACG808_000535"
     CDS             16013..17461
                     /gene="cls"
                     /locus_tag="ACG808_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFS5046188.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEIAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYILQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLIYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17798..18655
                     /locus_tag="ACG808_000536"
     CDS             17798..18655
                     /locus_tag="ACG808_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFS5046189.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18770..19690
                     /gene="pstC"
                     /locus_tag="ACG808_000537"
     CDS             18770..19690
                     /gene="pstC"
                     /locus_tag="ACG808_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFS5046190.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19690..20574
                     /gene="pstA"
                     /locus_tag="ACG808_000538"
     CDS             19690..20574
                     /gene="pstA"
                     /locus_tag="ACG808_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFS5046191.1"
                     /translation="MNAKRSDKIATGVLYVVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVSEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20589..21395
                     /gene="pstB"
                     /locus_tag="ACG808_000539"
     CDS             20589..21395
                     /gene="pstB"
                     /locus_tag="ACG808_000539"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFS5046192.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21411..22169
                     /gene="pstB"
                     /locus_tag="ACG808_000540"
     CDS             21411..22169
                     /gene="pstB"
                     /locus_tag="ACG808_000540"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFS5046193.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKNKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22182..22859
                     /gene="phoU"
                     /locus_tag="ACG808_000541"
     CDS             22182..22859
                     /gene="phoU"
                     /locus_tag="ACG808_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFS5046194.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            23435..24127
                     /locus_tag="ACG808_000542"
     CDS             23435..24127
                     /locus_tag="ACG808_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFS5046195.1"
                     /translation="MLLIGGKHGLFALGSLIINIVLLLLLLLIFTKIEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNLWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            24124..24891
                     /locus_tag="ACG808_000543"
     CDS             24124..24891
                     /locus_tag="ACG808_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFS5046196.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTTIELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24991..26559
                     /gene="liaX"
                     /locus_tag="ACG808_000544"
     CDS             24991..26559
                     /gene="liaX"
                     /locus_tag="ACG808_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFS5046197.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26856..27176
                     /locus_tag="ACG808_000545"
     CDS             26856..27176
                     /locus_tag="ACG808_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFS5046198.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            27176..27526
                     /locus_tag="ACG808_000546"
     CDS             27176..27526
                     /locus_tag="ACG808_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFS5046199.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27693..28631
                     /gene="hprK"
                     /locus_tag="ACG808_000547"
     CDS             27693..28631
                     /gene="hprK"
                     /locus_tag="ACG808_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFS5046200.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28646..29476
                     /gene="lgt"
                     /locus_tag="ACG808_000548"
     CDS             28646..29476
                     /gene="lgt"
                     /locus_tag="ACG808_000548"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFS5046201.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29520..30545
                     /locus_tag="ACG808_000549"
     CDS             29520..30545
                     /locus_tag="ACG808_000549"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFS5046202.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHCLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPVEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30560..31498
                     /gene="galU"
                     /locus_tag="ACG808_000550"
     CDS             30560..31498
                     /gene="galU"
                     /locus_tag="ACG808_000550"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFS5046203.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            31623..32063
                     /locus_tag="ACG808_000551"
     CDS             31623..32063
                     /locus_tag="ACG808_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFS5046204.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            32066..32668
                     /locus_tag="ACG808_000552"
     CDS             32066..32668
                     /locus_tag="ACG808_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFS5046205.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(32727..33833)
                     /locus_tag="ACG808_000553"
     CDS             complement(32727..33833)
                     /locus_tag="ACG808_000553"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFS5046206.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            34043..35044
                     /gene="ccpA"
                     /locus_tag="ACG808_000554"
     CDS             34043..35044
                     /gene="ccpA"
                     /locus_tag="ACG808_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFS5046207.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(35381..37726)
                     /locus_tag="ACG808_000555"
     CDS             complement(35381..37726)
                     /locus_tag="ACG808_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFS5046208.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            38005..38907
                     /locus_tag="ACG808_000556"
     CDS             38005..38907
                     /locus_tag="ACG808_000556"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFS5046209.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            39120..40895
                     /locus_tag="ACG808_000557"
     CDS             39120..40895
                     /locus_tag="ACG808_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFS5046210.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(40961..42547)
                     /locus_tag="ACG808_000558"
     CDS             complement(40961..42547)
                     /locus_tag="ACG808_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFS5046211.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            42797..43021
                     /locus_tag="ACG808_000559"
     CDS             42797..43021
                     /locus_tag="ACG808_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFS5046212.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            43163..44914
                     /locus_tag="ACG808_000560"
     CDS             43163..44914
                     /locus_tag="ACG808_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFS5046213.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            44914..46698
                     /locus_tag="ACG808_000561"
     CDS             44914..46698
                     /locus_tag="ACG808_000561"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5046214.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKTEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
BASE COUNT        15258 a         8181 c         9982 g        13506 t
ORIGIN      
        1 tatttggaat aagggactgc gacaagactt ttgtctcagt cccttttttt gcatattttt
       61 agcatttatt tcctaaaaaa tgtaagggga ttcctatatt tttaagtata cttgtttttc
      121 cttttataca ttttatggta ttatcatttt attagttatt ataaaacaat cgttagaaag
      181 gacgtcatta tgcaagtata cggaattgat gcggtgcttc gaatcgtgag tcatttggcg
      241 tttatctact tagccttctg gtctttacgt tcgttacgca ttgaaacttt tttcagacca
      301 atgcataata cgcaaattag aatggcaatc gtactttttt caattttttt agggtacact
      361 gcaagtaatt ttttcttaga agttatcgct ttatgccgaa atcttttcgt tagcctgtaa
      421 attttaaaaa aaggtactat tttaaaagca aggatgttat aatagaaaag attttgagta
      481 agtgtatgct cattggaatt ttttggaggg aacaacatgg aagagatcat cgtaagaggt
      541 ggcaatcaat taaatggaac agtaagaatt gaaggagcta aaaatgctgt tttgcctatt
      601 ttagctgcta gtttgttagc agaagaaggt attacgacat tagacaacgt tccgattctt
      661 tctgatgtct tcactatgaa tcaagtgatt cgtcacttaa acgtagatgt agattttgat
      721 gaacaaaaga atcaagtaac aatcgacgct tcaagacaat tagaaatcga agcgccttat
      781 gagtatgtta gtcaaatgcg tgcttcaatc gttgtgatgg gtccgctact tgccagaaat
      841 ggacatgcaa aagtagctat gcctggtgga tgtgcgattg gtaaacgacc aatcgatttg
      901 catttaaaag gatttcaagc tttaggagca aaaattattc aaaaaaacgg ctatatcgaa
      961 gcaattgcgg atgaattgat cggtaacacg atttatttag atttccctag tgtaggtgct
     1021 actcaaaata tcatgatggc tgccgtaaaa gcaaaaggaa caaccatcat tgaaaacgta
     1081 gctcgcgaac cagaaatcgt tgatttagca aacattttga ataaaatggg cgctcaagtc
     1141 tatggagctg gtactgaaac aatgcgtatt gaaggcgtgg atcatttaca tgcagtcaac
     1201 cactcaatcg tacaagatcg tatcgaagca ggtaccttta tggttgctgc tgcaatgaca
     1261 caaggcaatg tgctgattgc agatgctatc tctgaacata accgcccatt gatttctaaa
     1321 ttgatcgaaa tgggtgcaga aatcattgaa gaagaaggcg gagtccgtgt catcggacca
     1381 aaacatatct tacctacaga tgtaaaaacg atgcctcatc ctggtttccc tacagatatg
     1441 caagctcaaa tgacagctat ccaattagtt gcagaaggta caagtgtagt gactgaaaca
     1501 gtgtttgaaa atcgtttcca acatttagaa gagatgcgcc gtatgaacgc tcatgtcaaa
     1561 atcgacggaa atgtagcaat catggatgga aaccatgaat tgcaaggtgc agaagtatat
     1621 gcaacagact tgcgggcagc agcagcatta gttttagctg gcttgaaagc aaatggaatc
     1681 acacgtgtgc gtaacttgaa ttatctagac cgtggatatt ataatttcca tatcaaacta
     1741 cagcaattag gtgcagacgt tgaacgtgta gacatggatc aaacttctgc tgaaaaaaca
     1801 gcacaaacga ttgcttaagg aagtggctga atgagtaacc aacgatatat cttattaact
     1861 ttgataaaga ttttaatggt gatcttattg cttatccttc tttttgtggc cggaacgatg
     1921 atcggatatg gtgtcatcgg tggcggcaat ccatttaaag tatttcagcc aagtttgtgg
     1981 attcatattc gagacttttt tcattgataa ataagaatca cagtggttct agtcgatatg
     2041 aagtgagcca tgaagcagaa catgataaaa gaaaacagaa gggaacgttg acgttagtcg
     2101 cgatcccttt ttttagtaaa agaaaagaag gaatctaatt gatgaagaag atattgattt
     2161 atttagttag aggatatcag cgctttattt cgcctgcatt tccacctagc tgccgttatt
     2221 acccaacctg ttccaattat atgataaaag caattcaaac ccatggcgcg ctaaaaggga
     2281 cggtaatggg gactgcacgg atcttacgct gtcatccttt tgtcaaaggc ggtatcgatt
     2341 atgtaccatt gacattccga ttaacgcgaa ataagaaaga taaaaaagaa ccagattata
     2401 aaaaggttgt gaaaaatacg ggtagcactg agcgataaag aaacaatttg gaaaatagct
     2461 tcttatattt tgcaaaattg tgagttattg ccgaagtgtt ggattttgaa caccgtcaaa
     2521 aaaaaggttg tgaaaaatac ggctagcact gagcgataaa gaaaaagagg aaatttattg
     2581 tcaaagtaac aactccgcaa ctacttatat gggggacaaa aaatgatcga agaactaaac
     2641 aagaaagatt taccatggtc cttgttatta gatgcagatc ctgataaaga aaaagttcaa
     2701 gtatatgttt ccagagggag tggctggata tggaaagaaa aagaaaaaac aataggtgta
     2761 ctgatctatg tggcaaggga aaaagaatac gaaatcgtca atgtagcagt aaccccaacg
     2821 tatcaaggaa aggggattgg cggaaaatta cttgagacag cttttcagaa gctttcacaa
     2881 ctcgcttcca gtcaaactca gatcatcata cgaaccggca gtactagcag tgcggcgtta
     2941 catctctatc aaaaaatggg ctatgtggag attggtcgag tcaaagacta ttttatccat
     3001 cattactctg agcccatttt tgaaaatgga gatcaattaa gggatcaagt gattttagca
     3061 attcacttac ctaaaaatag ctttaactaa taaaatttca attcaagaaa atgctttcaa
     3121 atgctaaata gatacattac aatagcttta gcaaacaaaa aggagaatat ttttatggga
     3181 atattagttt ttgattttgg tggatcggct gtcaaatatg gttgttggga tggcaaagag
     3241 attaaaggta agggacaatt tgcaacgcct gaatcttggg aagaaatgaa agcgcaatta
     3301 tttcaagttt ataaaaaaat actcgtttct tttgaagtcg aaggaattgg aatcagctct
     3361 cctggagtag tgaataatga gaaacaggtg attgagggaa tcagtgcgat tccttatatt
     3421 catggattta atattttccg agatttggaa actctgtttc aacttccagt aacgattgaa
     3481 aatgacgcca actgtgccgg aatggctgaa ttttatgaag gtgctgcccg cgattatcaa
     3541 gatgttgctt ttgttgtggc aggaactggg attggcgggg cgttgttcac gaaaggacaa
     3601 atcaataaag gggcacatct ttacggtgga gaatttgggt tgatgttctt aaatggcgat
     3661 aagacattca gtaaacttgg tacagcggta cagatggctt ggcgttattg tgaacgcaaa
     3721 ggtctcgata aaaatgctta taccggaaaa gatgtctttg aattagcaga aactggagat
     3781 gcaatagcaa aagaagaagt tgagagtttt tatacgtatc ttacaaaggg attattcagt
     3841 attcaatttt cgtttgatcc agaagtgatc gtattaggcg gcggggtttc tgcaaaagaa
     3901 gggctcattg atgagatcaa aagccgaatg aagaagctga cagaacaatt cgatctgcat
     3961 gatttcgaac cgcagatttt attatgtgaa tacagaaacg atgctaatct tgtaggtgca
     4021 gcagcaaact atctcgctgt acgtcaaggg gagaattaag agctttagag aaggtgcagc
     4081 ttgcataaaa gaagtgaatg aaatgaatag gagattttta cctaaggaat gttttatttg
     4141 ctgcagtatg ataaaatatc tgagaggtga aaatgatgtc aaaaaaaaat aaagatattg
     4201 aagtgcgtat agaagaagtg aaaaaagata tcaaaggtaa gaattatgaa gtcactcagt
     4261 tatttatagg gaaaaaaatg attggtgaga tcttagccta tggaccaaaa gaatatgaaa
     4321 ttttctttgg tgaagaagat tttggaaaag aaaaaagtct tgaaaatgcg atagaaacag
     4381 tgattcgaca ctggaatctg cacgaataaa aaaatatcaa aaaaactttc aataaagggt
     4441 tgcaaaaatc tggaaaatta ggtattataa ctaagtcagg ttgatacaaa cctttcggag
     4501 gagtagcgaa gtggctaaac gcgacggact gtaaatccgt tccttagggt tcagtggttc
     4561 gaatccactc tcctccattt cattggggta tagccaagcg gtaaggcaag ggactttgac
     4621 tccctcatgc gttggttcga atccagctac cccagtatac cacaatcgta agctctcgtt
     4681 agttcgagag ctcttttttt tgctttgcta ccttgttcct tgaagattct gctgatttaa
     4741 gatagagtta agttaactac aagaattgga gggatccaca tgaaatggaa agaaaaagta
     4801 gacgaagcag ctgaaaagtt atatgatttg gtcaaatcag aaaaatacaa tattgaagtg
     4861 aatattccga aaaaaggcgg aaaagcggtc cgggtaaaat caaaacgccc aacgaatcat
     4921 acaaaaaaat ggatggctaa aaaccgctaa taataacaaa agatccataa gtcggcattt
     4981 tcatcgattt tagggtcttt tgttattttc taggttggac atatctaaaa aattgtttct
     5041 gacaatagga gtaggaagaa cgatgtgtgt ctaaatcctc tgttgatgga tagaaacctt
     5101 tatctttttc agttgtgaat caggtatata ccagttataa tagaagaaac gaaaggacgg
     5161 gtgtacgtta tgtcgaatca attacctgta tatatacaga ttcatgacca attaaaaaat
     5221 gaaatcgaac aaggtatatg gaaagtcgga gaccgtcttc cttcagaaag agaattagca
     5281 gtgaaattca atgtcagccg tatgacgttg agacaagcaa ttcaaaactt ggcagatgaa
     5341 ggaatcttag aaagaaagat cggttcgggt acatatgttg cacgagaaaa agtacaagaa
     5401 aaaatgtctg gtgcaacaag ttttacagaa attatggaat cacaaaatcg agtaccgtct
     5461 agtcggacaa tctcttattt cttgacttcg ccaagctcaa gtgaaatgga aaaactaaac
     5521 ttgagtaaag atgacacgat cttgcgaatg gaacgtattc gtttcgcaga tgatgtccct
     5581 atttgttttg aagttgccag tgtgccacaa aagctgatcg atggctacag caaagcagag
     5641 attactgaat cgttgtatcg cacgctagaa gaaaaaggcg ggaaaaagat cggtccagcg
     5701 aatcaaacga ttactgctgt gttggcttct gagaaaattg ctgactattt agaggtaaaa
     5761 cgaggagatg cagttcttcg tttgcgccag atttcttatt ttgaagacgg tacgccgttt
     5821 gagtatgtgc gtacgcaata cgtaggaaat cgttttgagt tttatttgga aaaataaaaa
     5881 acaggtgatc cctgattccg tattagaaat aaaagagaat cagaatcacc tgtttttata
     5941 tatgatcagt taccagttgc tcgtgataag aaacaggacc ctcttcaaat tggacttgtc
     6001 cgttcagcag ctcaatgatg gattctttaa agtggtcagc acttttttca tctacagcac
     6061 agatgacgct aacttgatca gtatattgga tttcttttat aaaaaaatgt tggagttcaa
     6121 gataattctg taattttcct agttgcggat acgtgatcat cacagagatc tcctgttgca
     6181 aggtaccttc tacgattcca atgtcagaca atgcttgaga gaccgcatga gtgtatgctc
     6241 gtattaatcc gcctgtgccg agtttcgttc ctccaaaata tcgagtgact actgcagcta
     6301 cattgatcag ttgatttttt ttgaggactt ccaacatcgg tatgcccgcc gttccacttg
     6361 gttctccatc atcgctgctt cgctggattt catttttttc tccaatgatg aaggcgctgc
     6421 agttatggtt cgctttccaa tgttcttttt ttatttcttg aatgaaactt tttgcttcgt
     6481 cctctgattc tattcgtcta agagaacaga taaagcgtga ttttttgatc tcgatctcac
     6541 tttgtccatt ttcttttatt gtacgatagc gctcgatcat cttgtcacct cttttttcat
     6601 tttctttatt cagtataaat aaagttctag gttgtttcaa cactgtaacg gttgcgcaat
     6661 tgctgtagta tgcgataata aagacaaagg caggtggatc tggggatgca ggaacgaatg
     6721 aaaaagtacg atgcaattac gcattatttg aagaacaatg gcggctctca agtaacgttg
     6781 acctttaccc aatttgacga actgctgttt cccagtaacg gacttcccaa aacagcaagg
     6841 gaaagtacag attggtgggc aaatgattat aaacaccctg aaaaaggtgc ctatggctgg
     6901 ataaatgcag gctatgaagt agttgtcatc aatttggaca aagaatatgt cgtgttcaat
     6961 aaattagtga aatctagttg gctatttgat taaataaacc gcgtttagaa aacgttgaca
     7021 aatttctttc tttttatata atagagtaac agacgcgaag aaaacgtttg cgtagtttgt
     7081 tttaaaaagg agagaaaagt atggctaaaa aaacaatcat gcttgtatgt tcggctggta
     7141 tgagcacaag tttactagta acaaaaatgc aaaaagctgc tgaagcaaaa ggaatcgact
     7201 ctgatatttt cgctgtgtcc gcttctgacg cagataacaa tcttgcaaat aaagacgtgg
     7261 atgtgttgtt attaggccca caagttcgct ttatgaaagc tgattttgag aaacgtctgg
     7321 aaccaaaagg cattccttta gatgtcatca atatggctga ttatggtatg atgaatggcg
     7381 aaaaagtgtt agatcaagca atcacgctca taggtgaata aatagaacag tataactatc
     7441 gaacaagcga tccaaaaaat agatcgcttg ttttttcgta tattttttta gggcgaataa
     7501 tatgaatcat ttttggggta gaaggcttct gacgagagat attaaagcaa tagactgtga
     7561 ggaggtaaat ttaccaaatt atgagaagat cgcagcagtg gaacgtttga gcaactggat
     7621 tctttgtcat cgctgtggaa tgagaacgtc cgtcattgat ggacagcttg cagattacgg
     7681 ttatttttgt acacattgtc tatatttagg gcgatgcgac actcaacaag atttatattt
     7741 gtttgatcag cctgaagcaa aacctcgaga agtcgtcttt ttttggacag gacagcttac
     7801 tgaaaaacaa acggaaatct caaagagaat actttgccat tcagaaaaaa gacatcatct
     7861 tatttgggct gtaacaggtg cagggaaaac agagatgctt tatcctattt tagtggaaac
     7921 tttaaaagct ggaggaagag tagcgatttg tacaccgcga gtcgatgttt gtaatgaatt
     7981 gtttttacgc tatcgttatg tttttccaga agaaaaaatc acgttgctcc atgggaatac
     8041 agcaacggct tataccttta cttcttttgt tatctgtacg attcatcagc tataccgttt
     8101 ttatcggtcc tttgatttaa tagtgatcga tgagatcgat gcgtttcctt ataacgggga
     8161 tgcaggatta gctcatgctg tgcaaacagc gattaaacca gacggacgat tcatctactt
     8221 aagtgccaca cctgataaaa agttactaaa ggagatcaag cagacgtttg acatccacaa
     8281 acttgcttta cgctttcatc ggcgtttgct tcctgaaccc gtcttggttt tcatgaataa
     8341 ttggcgacaa aaatgcaaga acaagaagaa gataaaatct ttggtgcgga tcattcatca
     8401 attaattaaa gaaaaccata tccttttatt ttgtccaagt atcacgatga tgcatcgttt
     8461 gaaagccaca cttcaagagg ctcttccgag atatgagatc acagatgttt cttcaagaga
     8521 caatcagcgg gcggaaaaag tccagaaaat gcgagaagca aaatatgaca tactgttaac
     8581 cacgatgatc cttgaacgtg gagtcacatt tgaaagaata tccgttatcg ttttaggagc
     8641 ggatcatccg gtttttacta aatcttcttt ggtacaaatc gcaggaagag ccgatcgtaa
     8701 agggccttat acgaacagtc aagtatattt tttctatgaa gaaaaaactg cagctattcg
     8761 aaaagcttgt caggaaatca aagaaatgaa tgaagaaggg aagaagatac tgtgaggtgc
     8821 agttgctgca gtaaagatat cgtccaaaat ttaacattca gcgagattat tggtgtttcg
     8881 aaatacagat tacctcagac ttgttcagtt tgtcgcgcac aatttcattt attggaaaca
     8941 agaggctgtt ctggctgtca aaaaccaatc catttcaatc tagagagtac gaaaaagaaa
     9001 gtaaattcta tccccgttta ctgcagtgac tgtcaagaat ggaagaagaa atatccgacc
     9061 tatgattttc agcaccgtgc cttttttgtg tacgatgaaa atatgaaggc ctggctcaat
     9121 caatacaagt ttatgggaga tatccgacta gcaggcacct ttgcatataa atggacgcag
     9181 ataaaaaaag aatacccgac atacatctat tgtccaatac ctttatctga aaaaaggcga
     9241 acagaaagag gattcaatca agtaagcgag atgttgaaag cagcaagaat tccttttcag
     9301 atgctactca aaagagagaa gcatctgctg gctcaagcaa aaaacacgcg aaaggaaaga
     9361 ttggagatgc ctcaaccttt tgaacttgca gtcacctttt ctaagataca aaataaaaaa
     9421 atattgatta ttgatgatgt ctatacaaca ggacagactc ttttccacgc agcagattgt
     9481 ttgcagatcg cacagccgaa aatcatccga actatttctt tggcgagatg aattagatgt
     9541 ggaaatttga tagcgattta atttgcaaat aacctagagt tcgatataat agaattaaga
     9601 agagggaaag agtggtcctt ctccttcttg attgtggtag acgaaagggg taatttttat
     9661 gtttagatat aatgtacgcg gcgaaaatat cgaagtaact gaagcgattc gcgactacgt
     9721 tgagaaaaaa gtaggcaaac tagaacgcta tttcagtgac gcacccgatg caacggcgca
     9781 tgtgaactta aaagtttata cagaaaaaac agcaaaagtg gaagtaacga tcccattacc
     9841 ttatcttgta ttacgtgccg aagaaacttc tccagattta tacgcaagta tcgatttggt
     9901 tgttgacaaa ttggaacgcc aaatccgcaa attcaaaaca aaaattaatc gtaaatcccg
     9961 tgaaacgggc atgaatactg caaatgctgc tgctttattc aatgaagagg acgaaaacga
    10021 aaatgattct gaattagata tcgtacgtac gaaacgcctt tcattgaaac caatggatag
    10081 tgaagaagca gtcttgcaaa tgaacatgct gggacataac ttctttattt ttgaagatgc
    10141 agaaacaaat ggtacaagta tcgtttaccg ccgtaaagat ggaaaatacg gactgattga
    10201 aacagactga tcagccttac ttaaataaca acgaaaactt gcagcagcta ctgacagctg
    10261 ccgcaagttt ttttgagttt cctcctcttt tagatgaaat atgaataaat tatgaagttc
    10321 ttactttaat acgcttaaga tatttcaatt atctataact aatgataaaa tgaaaagtaa
    10381 agtctacata gtctggataa tacacagaag gaaaaggaga ttacacaacg catggccaat
    10441 ttgattcgtt cacttatcga aaacgataag aaagaactaa aacgtttaga tagcattgca
    10501 aaaaaagttg aagcatacgc tgaccaaatg gcagcattaa ctgatgaaca attacaggca
    10561 aaaactgctg aattcaaggg cagataccaa aaaggagaaa cattagatca attacttccg
    10621 gaagcttttg cagtcgtccg agaagcagca aaacgtgtat taggtttata tccataccac
    10681 gtacaattga tgggtggaat cgtgcttcac gatggaaata tccctgaaat gcgtactggt
    10741 gaaggtaaaa ccttgaccgc aacgatgcca gtatatctaa atgctctgac tggcgaaggg
    10801 gtacacgtag ttaccgtcaa tgaatattta gcaacccgtg actcgacgga aatgggcgaa
    10861 ctttataatt tcttaggatt aacagtgggg ttgaatatca actcaaaatc ttcggatgaa
    10921 aaacgcgagg cttataattg tgatatcact tatagtacga ataatgaatt aggatttgac
    10981 tacttgagag acaatatggt cgtttaccgc catcaaatgg tacaacgtcc gctgaattat
    11041 gccattgtcg atgaagttga ctcaatcttg attgatgaag cgagaacgcc gctgatcatt
    11101 tcagggcaag cagaaaaatc cacagcttta tatacaagag cagacaactt cgtgaaacgt
    11161 ttgaaagaag aagaagatta taaaatcgat gtacaatcaa aaacaatcag tttaaccgaa
    11221 gcagggatcg aaaaagcaga agaaacattc ggtttggata acttatacga cattgaaaat
    11281 actgcattga cccatcactt ggatcaagca ttgagagcca actatatcat gcttttagat
    11341 atcgattatg ttgtccaaga cggaaaagta ctgattgttg accagttcac tggtcggatc
    11401 atggatggac gccgttattc agatggactt caccaagcaa tcgaagctaa agaaggcgta
    11461 gagatcgaag acgaaacgaa aaccatggcg acgatcactt tccagaacta tttccgtatg
    11521 tacaagaaac ttgcgggaat gactggtaca gctaaaacag aagaagaaga attcagagaa
    11581 atttataaca tccaagtata ccagattccg acaaatcgtc cggttatccg tgatgatcgt
    11641 ccagatttgc tttacccaac attacaaagc aagttccacg ctgtcgtgca agatatcaaa
    11701 gatcgctacc gcaaaggtca acctgtgtta gtcggaactg ttgcggttga aacatctgag
    11761 ttattatcag atatgctgaa tcaagaacgt attcctcatg aagtgctgaa tgcgaaaaat
    11821 cactttaaag aagcagagat catcatgaat gctggtcaaa aaggtgctgt gacgattgct
    11881 accaacatgg ctggtcgggg aacagatatc aagttaggac ttggggtaag agaacttggt
    11941 ggtctagcag ttatcgggac agaacgacat gaatctcgtc gtatcgataa ccagcttcga
    12001 ggacgtgcag gacgtcaagg agaccctggt atgtcacagt tctacttgtc attggaagat
    12061 gatttgatga aacgattcgg ttcagaacga atcaaagctt tcttagatcg gatgaagatc
    12121 ggtgaggaag atgctgttat ccaaagcaag atgctgacga aacaagtaga atctgcacaa
    12181 aaacgtgtag aaggaaataa ctatgacaca cgtaaaaacg tcttacaata cgatgatgtg
    12241 atgcgtgaac aacgtgaagt aatctataaa cagcgacaag aagtaatcat ggaagaaaaa
    12301 agcttatcca agccactaat gaacatggtc aaacggacaa tcagtcgtgt agtagatgcg
    12361 catacacaat tagagaaaga aaattggaat ttggaaagta tcatcgattt tgccggcagt
    12421 actcttgtcc atgaagatag tattactctt gcagatatcg aaggaaaaac agctcaggag
    12481 atcaaagagt atctaaacga acgagcacaa gaagtattta ataccaaagc ggctcaacta
    12541 aatggtccag agcaactgtt ggaatttgaa aaagtcgtga tcttgcgtgt tgtggactct
    12601 aaatggacag accatattga cgcaatggat caattacgtc agtcaatcgg cttacgtgct
    12661 tatggacaga acaatccatt agtcgaatat caaacagaag gctataagat gtttgaagat
    12721 atggtaggcg ccatcgagta tgaagtcact cgaatcttta tgaaagctga gatccgtcaa
    12781 aacgtacagc gtgaacaggt ttctcaagga acagcttctc actcagaaga cggagatgcc
    12841 aaagaaaata gtaatactag cgcgaaaaaa cagccaatcc atgtgaacaa ggtcggaaga
    12901 aatgatcctt gtccttgtgg aagcgggaaa aaatataaga actgtcatgg aaaaaatgca
    12961 aactaatttc tgacagcagg attggtgaga caaattcttt ttttaagaat tcgtctcacc
    13021 ttctttctgt tatttgctga ttctgttaga ctagatataa tgaaaaaagt atagtgaaag
    13081 aaggaaaaaa catggaaata tccgaaattc gaaatctcct gacagaaatg caggaaaagg
    13141 tcacaagttt caggaggtct ctttgactta gatcagctgg aagaagacat cgcggaagca
    13201 gaagcaaaaa tggctgaacc tggtttttgg gatgacagcg aagccgcaca aaaagtgatc
    13261 agtgaaaata acgctaataa agaaacctat gaccaattcc attctcttgc cgaagaacta
    13321 gaagaaatcg aattgatggt ggagatgctg caagaagaac ctgatgcagc aatgcaagaa
    13381 gaagcagaaa cacgtatcaa tgcattagat gaaaaaatga agacgtatga attatctatg
    13441 ctgttagacg gaccttatga tcgaaacaat gcgatcgtcg aattgcatcc aggggctggc
    13501 ggaacggagt cccaagactg gggcagcatg cttttacgta tgtatacacg ttgggcagag
    13561 cagcatggct atcaagtaga aacactagat tatcaagcag gtgatgaagc agggatcaag
    13621 agtgtgaccc tgctgattaa aggacataac gcttttggtt atctaaaatc agaaaaaggt
    13681 gttcatcgcc ttgtacggat ttcgcctttt gactcagcga aacgacgtca tacttctttt
    13741 tgttcggtag atgtgatgcc agagcttgat gaaacgatcg atatagaaat caatccagat
    13801 gatttgaaaa tcgatacgta ccgagcaagt ggtgcaggtg gtcagcacat caataaaaca
    13861 gaatctgctg tacggatcac tcacttgcct acaggaacag tagtagctag tcaggcacaa
    13921 cgctcacaat tgaaaaaccg tgagcaagcg atggggatgt taaaagctaa gctttatcaa
    13981 ttagaagtgg aaaagaaaga acaagaagca gctgcacttc gaggagaaca aaaagaaatc
    14041 ggctggggtt cacagatccg ttcttatgtt ttccatccct attctatggt aaaagaccat
    14101 cggacaaacg tagagacagg aaatgtccaa gcagttatgg acggtgacct tgatttattt
    14161 attgacggct atttaaaaat gcatctacaa taagaaccca ttgttaaatt gaaataaaat
    14221 tgtaagattt tgcaagcatg ttgaaacatt gccatgctat aatagctaaa gactgaaaaa
    14281 gatatggaga gaacgccatg attgaaatga aggatgtaat gaagaagtac tcaaatggta
    14341 cgaccgctat ccgcaatctc tcgattgtca tcgaacaagg agagtttgtc tatgtggttg
    14401 gtccgtctgg agccggtaaa tctacattca tcaaattgat gtatcgagaa gaaaaagctt
    14461 caaaaggaac tttgaacgtc gcaggtcatg atctaatagc aatcaaaaac cgcgaagttc
    14521 cttatctacg cagagaaatc ggcgtggtgt tccaagacta taaattatta ccaagaaaaa
    14581 cagtatatga aaatgttgcg tacgctatgc aagttatcgg cagaaaacct cgagatatca
    14641 aaaaacgagt aatggaagtg ctggaccttg ttggtttgaa acacaaagtt cgagtctttc
    14701 ctagcgaatt atccggtggg gaacagcaac gtgtctctat tgctcgggca atcgtgaata
    14761 cgcctaaagt acttatcgcg gatgaaccga caggaaatct ggatccagaa aactcatggg
    14821 aaatcatgaa attattagat cggatcaatg cccaaggaac tactgtcgtg atggctacac
    14881 ataacagtac gatcgtaaat acgattcggc accgagtaat tgctatcgaa aacggccgca
    14941 ttattcgaga tcaagcggaa ggggaatatg gatacgatga ttagaacctt tttccgccat
    15001 ttattagaaa gtatcaagag tttgaaacgt aacggctgga tgacagttgc ttcagtcagt
    15061 gcggtaacga tcacgttgac tttagtcggt gtattcatgg cagttatcat gaatgcaact
    15121 aaattagctc aggatataga aggaaacgtt gatgtatctg tattcgtaga tatcggtaca
    15181 aaagacgaag agatgaaaaa gctagaaaaa gaattgaatg acttagatca tgttaaaaaa
    15241 gttgaatttt caagtaaaga acaggaattg aaaaaaatcc aaaacgaaat gggtgattcc
    15301 tggaaattgt ttgaaggtga caataacccg ttatatgatg tgtatgtagt aagtgctact
    15361 gatccttctt atacaaaaca agtagcagaa gaagcagcag atttgaagaa tgtttctcgt
    15421 tctgattacg gtggagcttc ctcagatcgg attttccaaa tcgctggtgc agttcggaca
    15481 tggggattag ttgcagcggc attgctgctg ttcgtagcga tgtttctcat ttcgaacacg
    15541 attcggatca cgattttgtc tagacaccgt gaaattcaaa tcatgcgttt agtcggtgca
    15601 aaaaatggat atattcgctg gccgtttttc cttgaaggtg gctggatcgg tctgatcggt
    15661 gcgattcttc cgattttgat catcacattt ggttatgcat ggtttttcca attgatcaat
    15721 ccatcattat tacgttccca ttattcattg attcatccac aaaatatcgt ttggaaaatc
    15781 aatcttttaa tggtcggcat tggtgtgatc attggttcac ttggctcgat catttctatg
    15841 agacgtttct tgaaattcta ataccacaat ataaataaaa tgagaaagtt ggacagtttc
    15901 gttccagctt tcttttttta ttggctagga gaatagaagg aagcggcttt tttcccttta
    15961 ctttaaaaaa caacttgggt attcttaagt tggtagagga agaggtgaag ccgtgaaaat
    16021 tatcgttgac aattttttta cgatcctttt gattatgaat attttgcttt cctttatcat
    16081 cgtttttcga gaaagaaagg aaactgctca aacatgggca tggctgctcg ttttgatgtt
    16141 cattccagtt gtaggattta ttttatatat ttttttagga agagggattt ccaaagagaa
    16201 aatctttgat ttgaagatcc aagggaaaat cggtaagaac ctggaaattg aagaagacaa
    16261 acaggcattg atgcgagggc tttatcctca tccgcctact ggacaagtag atgtgaaaca
    16321 attgatctat atgctcacgg tatttgaaag tacgctctat acgactaaaa atgaaatcac
    16381 tttatttaca gatggtcgtg aaaaattcga tgcgctgatt gaagatatca agcaagcaga
    16441 agaccatatc cacttccagt attatattta tcgcagtgat gcattaggtg aagaagttcg
    16501 agatgcattg acagaggcgg caagacgagg ggtaaaagtc agagtccttc ttgatgcatg
    16561 gggttccaca caagtttctt ccagcttttt tcagaatttg aaaaaagcag gcggagaaat
    16621 cgcatttttc tttcctctat ttgttccgta tattaatcct cggatcaatt atcgcaatca
    16681 tcgtaaaatc gtagttattg atgggaaaat cggatataca ggtgggttca atgttggaaa
    16741 tgaatatcta ggattggtaa agaaatttgg ttattggcga gacaatcatt tgcgtattta
    16801 cggagaagcg gtgtatatct tgcaaaaccg ttttttgatg gactggaatt ctcagcatac
    16861 aaaagaagta ggctattctc ccaaattttt tccgtccatt cactctactg gtgatatagc
    16921 tgttcaaatc gtgacaagcg gaccagatac cgaacatgaa caaatcaaaa tgacttattt
    16981 gaaaatgatt tcgttagcca aacgggagat attgatccaa acgccttatt acataccgga
    17041 tggatcgatt catgaagcac tgaagttggc tcttctttca ggcgtaaaag ttcatatcca
    17101 aatacctaac aaaccagatc atcttctcgt ttactgggca acgtattctt tcgctgcaga
    17161 gctgatcgag tatggagcaa ggatcgaaac atatgagaat ggatttattc atgcaaagac
    17221 gatgattatc gatggtggca ttgtttcagt tggctcagca aatatcgatg ttcgttcatt
    17281 tcgcttggac tttgaggtca atacactgat ttatgatgaa cgaatggctt cacgtgtaag
    17341 aaaagcattt tttgaagact cgaaaatttc tacgcatctc acaaaagaaa tgtatgaaaa
    17401 ccgaggaata atcatcaaga tgaaagaagg attagcaaga ttgatttcgc cgctccttta
    17461 aaacacaaaa aggacatgag aaccatcaac agatggagat ctcgtgtcct tttctaacgg
    17521 ggccatttct gagagtacgt cagaaatgga gaaagcgtct cgcatgagcg aaattaactt
    17581 tggggtatct cttgtttttt ggggaaagca agaaataagc taatatatgc tattaaaaga
    17641 gacacatttc aaagccagta tagcatagaa acttttaatg agatagtttt ttttacaatt
    17701 atgaggattt attttgttat ttaatgattt tgattcgttt tcatcaagta atgaaaatga
    17761 taaaataaac atactttagg attaaaggag atttctaatg aaaaaactta aattattctt
    17821 accaataata ggattagcta ttcttttatc aggctgttca aagtggattg atcgaggaga
    17881 atccattacg gctgttggct cttctgcttt acagccttta gtagaaacag ttgctgaaac
    17941 ttaccagaca gaaaatcctg gaaaattcat caatgtacaa gggggcggaa gcggaactgg
    18001 tttgtctcaa gtacagtcag gagcagtcga catcggaaac tctgatttgt ttgcggaaga
    18061 aaaatcgggg atcgatgcct cagcattagt cgatcatcga gtagcagttg ttgggatcac
    18121 tcctattgtg aacaaggata ttggcgtaaa agacatcagt atggaaaacc tgaaaaaact
    18181 atttacaggc gagatcaaaa actggagtga agtcggtgga aaagatcagc cagtagttat
    18241 attgaaccgt gcttcaggaa gcggaacccg tagcacgttt gaaaaatggg tattggacgg
    18301 agaaacagct gttcaggcac aagaacaaga ttcaagcgga atggtgcgcc agatcgttgc
    18361 cgacacaccc ggtgcaatca gctatgttgc tttttcttat gtttccgatg atgtagatac
    18421 gttgaatgtt aacggtgtag aaccgacaga tgaaaatgtg acaacgaaca aatggacgat
    18481 ttgggcatat gagcatatgt acacaagaga tcatccaaaa aaactgacaa aagaattttt
    18541 agactatatc ttatctgatg aagtacaaga taacatcgtt ggagaactag gttatatccc
    18601 tgtttctcat atgaaagtcg aacgtgattg gcaaggaaat atcgttaaag agtaatcttt
    18661 acacaaaatt tacaaactta gctaaacttt tacatgttat taaaaccaaa ttgatgttca
    18721 atttgtacac tgagtgagta tgttaaacct gattaagggg gagtcatctg tggaagatgt
    18781 gaaaaaagta ttattaacaa aatcaaaacg ttcaaaaatg gaacagcgtg gaaaattcat
    18841 cagtttttta tgtatcgcgc tgatcgtgtt ggttgtatta tctatttttt attttgtagc
    18901 tagtaaagga ttggccacct tttttactga taagatcaat gtatttgatt ttctgtttgg
    18961 tacagattgg aatccaagcc aaatcggagc aaatgggaaa ccaatggtag gggcattgcc
    19021 tatgatcaca ggttcgttta ttgtgacctt tttatctgct attgttgcca caccatttgc
    19081 cattggtgca gcagtattca tgacggagat ctctcctaaa aacggaacga agatcttaca
    19141 gccggtcatc gagctgcttg taggtattcc ttctgtcgtt tatggtttta tcggtttatc
    19201 agttatcgtg ccttttgtca gaagtatttt tggcgggaca gggtttggta tcttagctgg
    19261 tacatttgtg ttgttcgtta tgatcttgcc gactgtcaca acgatgactg ttgatgcatt
    19321 aaaggcagta ccacggcact atcgggaagc ttcattagcg cttggtgcca caagatggca
    19381 aacaatctac aaagtcgtgc tacgagcagc cgtgcccggg attttaacag cagtcgtttt
    19441 tggtatggct cgtgcgtttg gtgaagcctt agctatccaa atggtcatcg gtaatgcagc
    19501 attgatgcca acaggactta ctactcctgc ttctacattg acgtccatct taacaatggg
    19561 aatcggaaat acaatcatgg gaacagttga aaataatgtg ttatggtcat tagcgctgat
    19621 cttgctattg atgtcattat tctttaatat cgtgatccgt atgattggga agaaaggaga
    19681 gctgaaataa tgaacgcaaa acgctcagat aaaattgcta caggtgtttt atatgtagtt
    19741 tcggggatca tcgtattgat tttagcagct ttactacttt atatcttagt tcggggaata
    19801 ccgcacattt catggtcctt cttgaccgaa ccttccaaag cctaccaaga aggcggtgga
    19861 atcgggatcc agctatttaa ctcgatttat ttgcttttga tcacgatgat catcagtttt
    19921 cctatttctt taggtgcggg tatctatctt tcagaatatg ctggaaaaaa ctggctgaca
    19981 gatgtgattc gcacgtcgat tgaaatcttg agctcattgc cttctgtcgt tgtcggttta
    20041 ttcggtttct tggttttcgt tattcaattc cagtatggtt tctcgattat ttctggtgca
    20101 ttggctttga ctgtattcaa cttgcctctt ttgactagaa cagttgagga gtctttacaa
    20161 gcggtccatt acacacaacg agaagcagga ttggctttag gtttgtcacg ctgggaaaca
    20221 gtgataaaag tggtggtttc agaagcgaca ccagggattt tgactggtgt gatcctaagt
    20281 tctggaagaa tttttggtga agcagctgca ttgatctata cggctggaca aagtgcacct
    20341 gcgttagatt ttacgaactg gaatccatta agtgtttcaa gcccgatcag cattttccgt
    20401 caagctgaaa cattagctgt ccatatttgg aagatcaata cagaaggcac gatgcctgat
    20461 ggtgtttctg tttcggctgg cgcttctgcc gtgttgatca ttgctgtttt actgttcaac
    20521 ttcggcgctc gtgcgattgg caaacgatta taccgaaaaa tgacttcagc ctaaggagaa
    20581 gaagcaaaat gaaaaaatat aactggaatg aaacaaatgt catcaccatg ccaaaagaga
    20641 aagaagtagc actatatacg gatgatctac atgtctggta tggcgacaat gaagcaatca
    20701 aaggagtgga tctggaattt gaaaaaaata agatcactgc actgattggt ccttccggct
    20761 gcggaaaatc tacgtatctt cgttcgttga atcgaatgaa tgatgggatc gctaatacga
    20821 aaatcactgg gaaaatcatg tatcaaggcg tagatattaa tgcgcctcaa gtggatgttt
    20881 atgagatgcg caaacggatt ggcatggttt ttcaacgtcc taatccgttc agcaagtcga
    20941 tttatgacaa tattacattt gctttgaaac agcacggcga aaaaaacaaa aagaaattag
    21001 atgaaatcgt tgaaacaagc ttgaaacaag ctgccttgtg ggaccaagta aaagacagct
    21061 tggacaaaag tgccttggct ttatctggcg ggcaacagca acgtttatgt attgctcgtg
    21121 cgatcgcgat gaaacctgat atcttgctgc tagacgagcc ggcaagtgct ttggacccta
    21181 tttcgacagg aacggttgaa gaaacacttg taaacttgaa agaagactat acgatcatca
    21241 tcgtgacgca taacatgcaa caagcggctc gtatcagcga ctatactgcc tttttctatc
    21301 taggaaaagt aatggaatat gacaaaacta acaagatatt taccagacca aaaattcagg
    21361 caaccgaaga ttatgtgtcc ggtcattttg gttaagacgg aggcctcaac atgacaaaag
    21421 aaattatttc atctaaagat cttcatctgt attatggaaa gaaagaagca ctaaaaggga
    21481 tcgatctaag tttcaatcaa ggggaaatca ctgcgatgat cggtccttct ggttgcggga
    21541 aatccactta tctgcgttct ttgaaccgga tgaatgacct gattccgaat gtaacgatca
    21601 ccgggagtgt tttatataaa gacaaagaca tctatggtcc aaaaacagat gttgttgaac
    21661 taagaaaaga aatcggcatg gtattccagc agccaaaccc atttcctttt tctatctatg
    21721 aaaatgtcat ctatggattg aaattaaaag gggaaaaaaa taaaaaagtc cttgatcaag
    21781 tagttgaaga aagcctgaag gcagcgtctg tttgggatga tgtgaaagac aaattgcata
    21841 aaagtgcttt atctttatcc ggtgggcagc agcagcgtgt atgtatcgca cgtgttttgg
    21901 cagttaatcc ggaaatcatc ttattggatg aaccaacaag tgcgcttgat cctgtttcta
    21961 ctgggaaaat cgaaagtatg ctgttagaat taagagagcg gtatacgatg atcatcgtga
    22021 cccataatat gtctcaagct tcacgtattt cagataagac ggcattcttt ttagatggtc
    22081 acttgatcga atacaatgat acgaagaaga tattcatgaa tccagaaaaa caagagacag
    22141 aagattatat ttcaggtcga tttggataag gaggaaacgg aatgctacgt acacaatttg
    22201 aagaagaatt gctgaatctc cataatcagt tctacgaaat ggggatgatg gtgagcagcg
    22261 ccgtgcacaa gtcagttcgt gcatacatca aacacgataa aatcttagcc caagaagtta
    22321 tcgaaaacga tataaatatc aataacatgg aaacacgctt ggagaagaaa agttttgaaa
    22381 tgatcgcatt gcaacagcca gttacaactg atttgcgtat gatcattacg gtcatgaaag
    22441 ctagctcgga tttagaacgt atggccgatc atgcggtgtc tgttgctaaa tccacgattc
    22501 gcgtaaaagg gcagacgaga ataccggaaa tcgaaaaaga gatctccgat atgtccgatt
    22561 atgtgaaaaa aatggtggac aacgtcctag ttgcttatgt caaaacagac gaaaaagacg
    22621 cacgtacgat cgccaatatg gatcaaagag tcaatgacta tttcaatcga atctataatg
    22681 caacaatcaa aaatatgcag gaaaatcctg agacagtcat cagtggaaca gactacttga
    22741 atgttgccag ttatctagaa cggatcggag actatgtgac aaacatctgt gaatggatcg
    22801 tctacttggc tacagggaaa atcactgagt taaatacgaa ccacaatgag attttctaaa
    22861 ctaaagcggt gattctgccg tttgtttagg aaaagaggtt gtgaaatcag cggttgtgaa
    22921 aaagctgttc tgcatcaagt aataagaaca gtcaaacaaa aatagctcct catatttttc
    22981 atttggctgg acttattacc gcagatgcaa gcttttgaac actgtttatt cgggaaaggg
    23041 tttgtgacaa aatatgtcac aaaccctttt catcatttca tagaaaggat aaagaatgca
    23101 aaaaaaaatt agcatcatca ctatattttt tatttgtgtg cttacttcat gggttattca
    23161 gcatcaagct gctttatatg aagtacctgt catcgagatc gaaaaagtac gaataaaaga
    23221 ggatcatcaa ttgattacag gaagactgat gaacacgaat caaaaaggag aatcgattta
    23281 tttatcggtt ccttatcaaa aaaatcaaat agaaacagca aatataaaag cgagacaaat
    23341 gtatctagtc caaatgcatg gagatgaatg gcagctagta gcaaaaaaaa atgatggctg
    23401 gcttttcttc ttcacaagta tcttcattgg cgccatgttg cttattggcg gaaaacatgg
    23461 tctatttgct ttaggaagtc tcatcatcaa tatcgtttta cttttacttt tgttgctcat
    23521 ctttactaaa atagaaggga tttctctatt aaatatatct atcctgttcg tcgttatagg
    23581 gatgattatt agcatattgg ctttagatgg atggaataga agcagtataa tcaaaatttc
    23641 agctgcagtt acgagtgttt ttttagcatt ttttatttgt cttttgacaa tgaatctttg
    23701 gaaagaccaa ggactgcgct atgaggaact gaattatctg acacgaccgt atcgttctgt
    23761 ttttctttct agcgtactga ttggaacagc tggaggaaca ctcgataccg ttatcacagt
    23821 cattgcgaca ttagaagaat tgactaagcg acaaccagaa ataacagcga aacagttatg
    23881 gctttcggga aggcaagtgg gtcgagatgt gattggctcg atgaccaata tccttttgtt
    23941 cgtctatatc agtgggtcag ttccatcgct tcttttatat ttaggaaatc aatggtcgtt
    24001 caaagaaacg atagaacagc atttatcatt ggaattttta cgcgtcattg ctggcagctt
    24061 aggaatagta ttatcgatcc ctatctcggt cctctttttt ctgactgtaa ggaggctgaa
    24121 acgatgaatg tacttattat acttgttggg attttagcaa tcagcgtgct acttattggc
    24181 ggtacgcaag gtatgtatat tcttttaggc ttattcatca atttagggat ctttttcttg
    24241 ttgttattcg gttatcatca gaagtggccg atactggcat tgtcggtcat aggattttta
    24301 ttgattgctg tggttatctt attttttatc aacggttata atctaaaaat gcgtgcagct
    24361 tttgtgagta tactgatctt tttattttgt ttcctactat tgcttcctat cactgatttt
    24421 ttagcaatcc aaggttttac aacgatagaa ctagaagaat tatctggctt agataagact
    24481 ttagcgattg attttagatt acttacgaga tcgttgcttc tgattagtct tagtggtgct
    24541 gttttagatg cgagtgtagc aatttctagt gggacattcg aagtatatca ggcaaatccg
    24601 caattatctt tttaccaatt gcgccattct agtttcgcta ttgctaaaaa aatattggcg
    24661 tcaacagtaa tgacacttct ttttgcattc atgggaagtt ctttagcgtt gatcctctgg
    24721 tttattgatt tagacattcc ttttcagcaa gtgatcaatg aaaaatcatt tgttctagaa
    24781 tatacgatgg ctattcttac cacattgtcc gcactacttg ttcttccttt aacctgcgtg
    24841 actacagcat atctctttac aaaaaagaaa gaacaactca agatggagta aaagttacaa
    24901 cttaagtcct atgacatttt tactgaatca gtgcataata tggatgtaag gaaaagcttg
    24961 caaacaattt tgcttaagga ggcaatttcc atgaaagaaa gagaacgtat tttagattta
    25021 gtgaaaaaag gaatcctttc gactgaagaa ggcttagatt tattagaaag catggcaact
    25081 gaaaaagacg aaaagcaaat caaaaaagaa gcagacaagg taactgctag tcataaagaa
    25141 aaagatcagg caagtcaatt gatcgataaa cttgaaaacg gcgaagaaga aatttctgag
    25201 ccagttgatc caaaggaaag agaaagacaa gatcaagaaa acttagaaaa gatcttagat
    25261 gaactagcaa ctgaagcgaa taaaacttcg gcgcgtctag atgaagtaaa tgctcaattc
    25321 gctgacaata aggcagcacg caatgagaaa caggaagcat tgatgcagct gaatacaaaa
    25381 gaagaattag gcgagttgac agaagaagaa ttagcacagc gtcagacgtt agaagcagaa
    25441 atcaaagagt tagaagctag tggcgataca ctgttagaag aacaattgaa attagaagca
    25501 gaattaaaag atatccgtaa gaatcaatgg tctgagaaaa aagaaacgtt tactgataag
    25561 tttgaattgc cagatgattg gaaagatcaa gcaacagata cactgaatca agtaggcgaa
    25621 aaaatgtcag aagctggatc acagttagga aaattcttga agaaaacatt ccaaactgtt
    25681 tcagaaacag tgaatgacaa catggaatgg aaagatgtca gcttgcgtgt tcctgggatt
    25741 gcgacaacga aatttgagca tgaattttac tatgaagctc ctgcagcaag tatccttgat
    25801 atcaaggcag caaacggaaa cgtgacgtta aaaacgtggg acagcaacga tgtgaaagtt
    25861 gaagctaaga tcaaacttta cggaaaaatg ggtgctgaac catttgaagc attctctgaa
    25921 cggagccaga ttgaggttaa tgaagatcat atctccttcc aaattccaaa taaacgtgtg
    25981 cgtgcagacc ttgttttcta cttacctaaa cgagtatacg accacgcagc tattaaactt
    26041 ctaaatggca acatcatgat tgaaacattg gaagctaaag atatttatac aaaatctacg
    26101 aatggtaaca tcatcgtgga ccaattaact gcaacgatgc tggaagtaga aggtgtaaac
    26161 ggcaatatcg atattcgaaa cgggaacatc ttagattcta ttatcgaaac agtaaatggt
    26221 acagtgacat ttggtgcgac agcagaaaac ctttctgtgt ctttggtaaa tggtgatgta
    26281 cgtcttacga ttaaagaaga taatttgaaa aaagtagaag caagttcagt gaacggaaat
    26341 gtcaaagtcg ctttaccaga tggcatcggt ttggaaggac atgctaaaac aagtcttgga
    26401 agtatcaaca gccgtctgtc caattacgaa gtagttcgtg agaaaaaaga acggacaaac
    26461 caaatgttgc aattccgtcg tgtttcagat ggtgaaatcg cgcaagtgca actttcaacg
    26521 acaacaggaa gtatctattt aaaagataca gataaataaa gaggttgtga aagagatgtt
    26581 cagccttgag caataagaag cgattttgta aaatagcttc ccctatttta caaaattgtg
    26641 ccttattgcc gataggctat ctcttgaaca ccgtttacca agagattatg aacttgccaa
    26701 ttatcgccga gtcacaagga ataatttcaa aaaaagctcg tcacgtttta tgaatatggt
    26761 aatttgtgac cgagaatttt gcaaaaagac accgattaag aagaaggttg tcacaacgat
    26821 tttcttcatt taaaatagta gaacagaggt gagacatgat gaaaaaaaag ttgacaaaat
    26881 ccaataaaaa tgttgttttg acagggactt tagcaggaat aggagaatac atcgggattg
    26941 atccgactgt tatccgtgtg ctttatgtat tcctgagctt agttgctttt ggaagtccaa
    27001 tcattttata cattttatta gctttgatta tcccatctgc gcgttctgca cgaacaaact
    27061 atggacataa caatgcatat tatgaaaaaa acaattatcg tgaacaaact cgtgaacaaa
    27121 caaaaaaacg caaagaagcg gaaaaaatcg atgatgacga atggagtgac ttttaatgtc
    27181 gtactttcaa cgtattttag ttaatacgtt aacattcata tcattggctg ttctttttcc
    27241 tgcgatggtc catgtgagca gtatttggac ggccgttctt gccagcttcg tattgtctgt
    27301 gttgaatatg tttgtcaaac cgattttgac gatactgtcc ctgcccttta cattagtcac
    27361 attgggattg tttacgtttg ttatcaatgc agcgattctt aaaatgacct ccttttttgt
    27421 gggagcagaa cattttagtt tttcttcatt cggtgcagcg ttgttagtag ctatcttgat
    27481 gtcgcttgtc aatgtaattg tcagcgaaca taacagtaga aaataaaaga aagctgtgcc
    27541 gaactatcgg cgcagttttt ttatttagaa tcaggaatgc gttttctatt acattggtaa
    27601 ttaaataaaa acgttttttt taattggtag tacttttggc tagacaatga taaaatgata
    27661 gagaacactc taatagaaat gaggtagcat gaatgtcaga agttgtaaaa gttagccaat
    27721 tagtgaaaaa attatctttg gaaatcgtga caggcgacga gaaaagctta gaccgtgtca
    27781 tcgtaactgg ggacatctca cgcccaggac tagagttgac tggttatttt aattattatt
    27841 cccataatcg tttacagcta tttggcagta aagaaatcag ttttgcggaa cggatgatcc
    27901 cagaagagcg tttgatcatc atgagaagat tatgtagtga agatatgccc gcatttatta
    27961 tttcaagagg attaccagct ccagaagaaa tgatccaggc agcaaatgaa aatgggatag
    28021 ctgtactgcg ttctcccatc tcaacttctc gcttattagg agaattatcc agttatttag
    28081 atggtcgcct tgcccctcga acaagtgtcc acggtgtttt agtggatgtt tacggattag
    28141 gtgtactgat tcaaggtgac agtgggatag gaaaaagtga aacagcgttg gagttgatca
    28201 aacgcggtca tcgattgatc gcagacgatc gagtagatgt ttatcagcaa gatgaattaa
    28261 cggtgatcgg agaaccgcct aaaatcctgg aacatctgat tgaaatccga ggaatcggga
    28321 tcattgatgt aatgaatctt tttggtgcga gtgcggtacg tggctctatg caagtccagt
    28381 tggctgttta tctggaagca tgggcaaaag ataagaaata cgaccgtctg ggctctgaag
    28441 acacgaccgt tgaaatcgcg gaagtaggca ttccacaaat aaaaatccct gtcaaaacag
    28501 gacgaaacgt tgcaatcatc attgaagtag ccgcgatgaa tttccgtgca cgtacaatgg
    28561 gctttgacgc aacgaaaaca tttgaagaaa gattaagtcg cttgattgaa gaaaactcag
    28621 gcaacgacta gcaaggagtg acaatatgtt agcacaaatc aatcgagttg cttttgagct
    28681 tgtagggatt cctatctact ggtatgcatt gatcatcgta tcaggtatca ttatcgccat
    28741 gtggcttagc agcagagagg ctgttcgggt aggattaaaa gcagatgatg tgacagattt
    28801 tatgttgtgg ggattgccta tctccattat tggcgctcgc ttatattatg ttttatttga
    28861 tcttccccag tatatagctg atccaatcca aatattcaat atccgttcag gaggcttagc
    28921 tatttatggc ggactgattg caggcgcgat cacgctgtat ttttttacta aatatcattt
    28981 tatttctttg tggacttttt tagatatcgc tgcaccaagt gtattattag cacaagcaat
    29041 cggtcgttgg ggaaatttca tgaaccacga agcatatgga ccagacacga cgcgaagctt
    29101 cttagaaagt ctgcatttac cgcaattcat cattgataat atgtatatcg aagggatcta
    29161 tcgtcaacca acatttcttt atgaatcact ttggagtttg attggattta ttttgctttt
    29221 gtttttacga aagaaaccac accttttgaa aaaaggtgaa gtccttttat gttatctaat
    29281 gtggtatagt ttcgggcgtt tctttattga aggattacgt acagacagtc tttacttttt
    29341 aggtgtgatc cgtatttctc aattattatc tgctgtgtta tttgtcggta cattgattct
    29401 atttatttgg cgcagaaaaa aaggaaattt accggattat gaccgctcat taggaaaaaa
    29461 tcaacaaatt atctgatttt tagagcgtta aaaatgaaaa aaatttcagg agtggtaaaa
    29521 tgaattatag agtagcggtt ttaggaccag gctcttgggg tactgctttg gcacaagtcc
    29581 tcgccgaaaa cggacatgac gttcggattt ggggacatcg tgcttcacaa gtagaagaaa
    29641 tcaacaccca acacacgaat caacgttatt taccagatca ttgtctaccc gattccatcc
    29701 aagcattcac caatatggaa gatgcagtaa aagacgcaga tgcggtgcta ttcgtcattc
    29761 caacgaaagc aatacgttct gttgcaaaag aactagtgcc taaattgaat acaaagccag
    29821 tgatcatcca tgcaagtaaa ggattagaac aggggacaca taaaaggatc tctgatatct
    29881 tgttagagga aatcccagta gaaaaaagaa aagctgttgt tgttctttct ggaccaagcc
    29941 atgcagaaga agtagcgaaa catgacatca ctacgatcac agctgcaagt acagatgcaa
    30001 aagcagcaaa atatgtacaa gagctgttca tgaataatta ttttcggatc tataccaatc
    30061 ctgatgtgat cggtgtagag atgggggcag ctttgaaaaa tattattgct attggtgctg
    30121 gcgctattca cggcttaggg tttggcgata atgcaaaggc agcaatcatg acgcgcggat
    30181 tagctgagat cagccgttta ggtgtagcaa tgggtgccaa cccgctgacc tttatcggat
    30241 taagcggagt aggcgatcta gtcgttactt gcacaagtgt tcattctagg aactggcgcg
    30301 caggcaactt gttaggacaa gggcagcccc tagaagaggt gctagccaat atgggaatgg
    30361 tcgttgaagg tgtgaatact acccaagcag cgatggaact agcccaaata atggaaatcg
    30421 aaatgcccat cactcaaaca atctatgaag tgctgtatga acaaaaagaa atcaaaacag
    30481 ccgcaaaaga aatcatgtta cgtgatggga aaaaagaaaa tgaattctca ttaacaattt
    30541 tttgaaggag cgaatgtata tgaaagtcaa aaaagcagtt attccagcag caggattagg
    30601 aacaagattt ttaccagcaa caaaagctat ggcaaaggaa atgttgccaa ttgttgataa
    30661 accaacaatc cagttcatcg ttgaagaagc gttgaaatca ggtatcgaag acatcttgat
    30721 cgttacagga aaagctaaac gaccaatcga agatcacttt gattcaaata ttgaattaga
    30781 aacaaaccta agtgaaaaag gaaagaccga attattgaaa ttagtagaag aaacgacaga
    30841 tgtcaacttg catttcatcc gtcaatctca tccaaagggt ttaggacatg ccgttcttca
    30901 agcaagagcc tttattggaa atgaaccatt tgtcgttatg ctaggcgatg atataatgga
    30961 agacgaagta ccattgacac agcaattaat cgatgattac gaacgtactc atgcttctac
    31021 gatcgccgtt atgcaagttc ctcatgatga aacttcaaaa tacggaatta tcaatccagg
    31081 agaagtattg gaagatgggt tatacaatgt caaaaacttt gttgaaaaac cagatccaag
    31141 caaagcacca agtgatttag ctattattgg acgttactta ttgactccag aaatttttga
    31201 tgtattgtcc gagcaacaac caggagccgg aaatgaaatc caactaactg acgcaatcga
    31261 tacattgaat aaaacacaac gcgtatttgc ccgtaaattc acaggtaaac gttacgatgt
    31321 cggtgacaaa tttggtttta tgaaaacaag tattgaatat ggtctaacac atccagaagt
    31381 aggtgcacct ttacgtgagt acatcctgtc tttaggtaaa acattagaag cagaaggaag
    31441 caagatcgat cataatccaa aaggaataac agaaatcaaa gaagaaaaga aaaaataacc
    31501 cttttttcta acgagcttca gctgctaatt gaataaaaag tagttgaagc tcgtttttat
    31561 ttgtttaaaa aacacgaaaa tgctatcctt atagaaaaga gagacaagaa gggagtttga
    31621 tcatgtcagg aggagaaatt gcaggattga tcgcagcaat cgcctttgcg atacttgtcg
    31681 tttttatcgt tcgtgtgttg ttgcaagttt ctaaaacttt agaaaaagta gatcagactg
    31741 tagcagaagc aaatacgacg atcgaaatcg tcacaaaaga cgtagatctt ttgtctagac
    31801 aagtagaagg cttactggtc aaaagtaatg aactgctctt ggatatcaat ggaaaagtcg
    31861 caacgatcga tccattgttc acagcagttg ctgatttaag tgaaagtgta tcagaactta
    31921 atcattctag taaaaacatc gcgacgaaag tcggcggaat cggtaaaacg acagctaaag
    31981 cgacggttgc cggaaaagtc ggtgggacag cgatgaaatt ttttaaaaac aaaaaacata
    32041 ctgaaacgac aggaggaaaa taaaaatggc taaaaaaggt ggatttttat taggtgcagt
    32101 agtcggaggc acagccgcag caatcgccgc gttattgtta gcaccaaaat ctggtaaaga
    32161 acttcgggat gaattagctg cccaagcgga tgatttgatg gatatggctt cagattacac
    32221 agatatggcg aaagaaaaat cgactgagtt aacaggtatt gcaaaagaaa aagcgcaaga
    32281 tctttctcaa caagcaggtg atctagcagg tagtgtcaaa caaaaagtaa gtgaatcagc
    32341 agatgaagca ggaagtgaat ctgacgatat cttgttaaat cttaaagaac aatcagctga
    32401 cttatctaat cgtttcaaaa aaacggcaga agatatgaaa aaacctgcag atgaattagg
    32461 aagtattgct aaagatactt cagaagatat catgattgat gtaaaagacg ctgctggtaa
    32521 agcaggagat actgtcaatg aaggaatgtc agaagcaaaa ccagttgcag atgactcaaa
    32581 agaaatgatg aaaatggcaa aagaagatgc gaaagaagct aaaaaagaaa taaaagatac
    32641 aatgacagat tcatctacaa atcactaaaa tcacaaattt atagaataaa aaggtgggag
    32701 cggactagct ctcacctttt ttactcttat ttttctaaaa caagtaattc ttttggtgta
    32761 gtcgtgaatg gcacacagcc atcgcttgtc acatatacac agtcttcgat acgtacccct
    32821 actttttcag gaatatagat tcctggttca agagagaagc acatcccttc ttcgattact
    32881 aagtcatttc cttctacaag ggaagggaat tcgtggacgg ttgttccgat cccgtgacct
    32941 aaacggtgat tgaaatattc gccgtagcca tagctgttga tcacgtttcg tgcgatttga
    33001 tcaagttcac ctgctgtcac acctggacgg acggcttctt gtgcggcaag ctgtgcttca
    33061 agagtgatgt tgtagatttt ttcttggaag tcggttggtt tttgataagc aattgtacga
    33121 gtagcatcac tgcaatagcc attccaaaca acgccaagat cgaataacac aagttcattc
    33181 ggtgaaatct tcgtatttcc tggtgtacca tgagggcttg cagcatttgc tcctgcaagg
    33241 actaatgtgt cgaaagacat ttgtgaaaca ccttgttttt tcaattggta ttcaatttct
    33301 gcaatgatcg cttgttcagt cacgccttct ttcactgctt taaagccaat ctcaaatgca
    33361 acatcggccc atttaccagc ttcgatcagt ttgtcttttt cctctagagt tttgatcagt
    33421 tgtagacgtt ggatgatgga agttagatca gtgtaagaat ccgttcccgt caagatatca
    33481 gagatagcat cgaagcgctc gacagtcaag gcgttctttt caatccctac attgccaggc
    33541 tgaccatagc gagcacgaag ttcttttccg atgatctccc aagggttttc gctgtctaaa
    33601 tagccacgga catcatacgc ccaactactg ttttgagcat cttcaacttc caaagcagga
    33661 gtgaaaagga aaggatcttg ttcaagaggg ataaaaagag ctagtacacg ctcatgagga
    33721 tcactttcgt atccggaaaa gtaagcgatg tggccagggt cgctgatata agccaattcg
    33781 acttggtgtt gtgccatcca ttgacgtagt tcattgattt ttacgttatt catttggcaa
    33841 cctactttct aaattaattt tgtattcaat aattgtacca ctgatttatt aaaagaccaa
    33901 cgttctaaaa actgaaaaaa gtaaaaaatt tcatgaaaac agagataaaa cggttgcaat
    33961 tcttgagatt atttgatatt ctattttaga aatgtaagac gtattttcag aaaacatata
    34021 aaatatgaat aggagaacac aaatggaaaa gcaaacaatc actatctatg atgtagcgag
    34081 agaggcaaat gtttcgatgg ctactgtttc acgtgtcgta aatggcaatc caaacgttaa
    34141 accagcaaca agaaaaaaag tcttggaagt tatcgatcgc ttggattatc gtccaaacgc
    34201 agttgcgcgt ggcttggcaa gtaagaaaac aacaacagtt ggtgtaatca tccctgatgt
    34261 cagcaatatg ttctttgctt ctttagcacg tggtatcgat gatgtggcaa caatgtacaa
    34321 atacaatatt atcttagcaa attcagatgg caacgaccaa aaagaagtca atgtattgaa
    34381 taacttactg gctaagcaag tggacggcgt gatcttcatg ggtcaccata tcacagacga
    34441 aatccgtgga gaattttcac gttcaaaaac tcctgttgta ttagctggct ctattgatcc
    34501 agatgaacaa gtaggtagcg taaacattga ttatgtatcc gcaacaaaag atgcagtaaa
    34561 caaattggca aaaagcggca ataaaaaaat cgcttttgtc agtggtgcgt tgatcgatcc
    34621 catcaatgga caaaatcgtc tgaaaggcta taaagaagca ttaagcgaaa ataacctacc
    34681 atatagcgaa ggattgatct ttgaagcaca atacaacttc aaagatggtc tatctctagt
    34741 cgatcggatc cataatagcg gtgcaacagc tgtctatgtt tcagatgatg aattagccat
    34801 cggtattttg gatggtttat tagaccgtgg cgtgaaagta ccggaagaat tcgaaatcat
    34861 cacaagtaac aattcactac tgacagaagt agctcgtcct cgtttaacaa gtatcacaca
    34921 accattatac gatttagggg ctgtatctat gcgcctattg acgaaaatga tgaataaaga
    34981 agaagtcgaa gaaaaaacga ttatcttgcc ttatggaatc gaagaaaaag gatctacaaa
    35041 ataataaaag gacaaccatt cagaggttgt gtgcctgccg tttaactctg cgttacaagg
    35101 agcaatttca aaaaacagca tctcatgttt gatgaatatt gtgactcgtg attcggagtt
    35161 ggcaggagaa caccgtttat ttgggaaaag gggttatgac aaaattttgt tataatcctt
    35221 tttttgtgtc ttaaaaaaga gagaaagtgg ttgaaattac atttatcgta tataaaacaa
    35281 tccgtgtata gatgctgcat tttgttcacc accatcaaaa aaataaggga ctgtgacaat
    35341 actattgtca caatccctta ttactgtgta ttctattaaa tcaatcatct ttctcatctg
    35401 attctttagt tgcttgattc gctcttgcat aagctgaagc ggtgttccag taatctgtat
    35461 agttttcatc ggtaccgcca acgccaaatt taaaggcgct tttcttcggt ccatttttcg
    35521 cccatagcga ttcaactgtt tttcctggtg tttggatgga ttttttattg acagtaactt
    35581 ttccaggtgt ttgaccagta aatccagata cttttttctt catcacatca gatgataatt
    35641 cgaatttctc atcagtgcca aaaatgtcag gttttgcttg gtaagcttga ttgatcagat
    35701 tggcaagata attagccgaa cgtatccttg ctttgctgtc cattccagta ttgtcatcgt
    35761 gccctgccca gctactgatg gtaatagacg gagtagagac aattagccat gaatctcggt
    35821 attcattggt agaaccagtt tttccgaccc aatctgcttt tcctagattt ccgtttaagc
    35881 ttgagatggc atctttgaat ggtgtagtga tttttgcatt gatcacacta cgcatcatgt
    35941 cattcatgat cgatgccgtt gcttccgaat agatccggac agagttgctt tcgtgtttat
    36001 aaattacatt tcctgcgtta tctgtgatgg aatcgatgat atagccttct tcatagacgc
    36061 cgccgtttgc taaagcctga aatccgttgg tctgttgaag cgtcgtgaca tctactgtac
    36121 ctaaaggagc agattcaacg ccccaattgt catttgccgg gtagttcatc tttgccaagt
    36181 agtgctcgta agcatattgt ttcgatccac cggaattcaa cacatcttga tataagtgat
    36241 aagccgggat attgttggac cattctagtg actcacggac ggtttggaag gtgttcgatc
    36301 cttcgttagt agcattgacg attttctcgc cagcattttc tccttcttgc caagttgttg
    36361 cataatctga tacacgggat tctgatccga taagccccat gtcgattgca ggtccataaa
    36421 caagaactgg tttgattgat gagccagctt gacgttctgt atcgaaggca tggttatttt
    36481 gattctgact gtaattccgt ccgccaacga atccgtagat gcggccagtt ctgttatcca
    36541 tcaatacatt tccggtttct acttgactgc cagcaccatc atctaacatg tatccataat
    36601 tttcaacgcc attttgcaat gcagcataga catctttatc gattgtcgaa tggattgtat
    36661 atcctttatt ttggatcgtt tgctgtgcct tttgataata agcgtcactg accgatgaat
    36721 cagacatatc agcgttgtct ttttcagcca attgttgtgc aatgattctt gttgcttcat
    36781 tcatcacagt ataatacaag aattctctgt cagtctgttc tgcgatttgt tgtggtagaa
    36841 agtcttttgt aaggtcatag gcttttgctt cttcgtattg ttctttcgta atttggcctt
    36901 cacgatacat actgaataaa acaaaatctt ttctggcaag gccggcagaa agatcttctt
    36961 ttagcgcgcc tgtgttcgtg tatggactat acgtgatcgg gctttggggt aatccagcga
    37021 tataagcagc ttgcggcagc gtcacatctt ttgcatctac accaaagatt ccttttgcag
    37081 cttcctggac tccagcaatg ttttgtccct tattattacg accaaatggt gagacattca
    37141 aatacgtagc aatgatctca tcttttgaaa agtatttttc tacttgtgca gaaagaatga
    37201 tctcgtttgc tttacgtttg aatgttgttt catctgtaag gatctgctgt ttgactaatt
    37261 gctgtgtcaa agttgatcca ccagaagagc caataccagt agcttcagat actaatgctc
    37321 gtaaaacagc tttaggaacg taaccttggt gtttatcgaa gtattcatcc tctgtgctga
    37381 taattgcagt tttcaataaa ggtgaaatct tatcagatga aatagtcgtg cgcatcagat
    37441 ctgtctggat ttttgatatc tctgtattat ctgcataaac aatctttgac gtttctgtaa
    37501 tgtttcctag ttctgtctgc agtgcttttt tagtcggcaa ttgtgtgtct tcgacgagat
    37561 aggcaaaata gccagcgcct attccaatac ctagagctgc gaacatcaaa agaagaacag
    37621 taataaaaac agtcaatgat tgaaaaactc taaaaataat cttaggaaca aaccattttt
    37681 ctttttttct tctattctgt ttttttcgag tatttttgcg tggcaaaagg gtcacctcaa
    37741 ctaatggttt tatttcgcta attatagcaa ataatagaac aaaaaaacag acaaggctgt
    37801 ttttggcagg aatttctttt ttaaaagagt taaaacgtga gaaaatgatc tttattataa
    37861 aagagttctt aagcttttct ttagagaaaa tttcagttac tatggagaac agggcaaaga
    37921 aaaatcaatc attttctttg tatatcatct taccaatata taggaattgt tagtaacaat
    37981 tgcctttcaa ggaggaatcc atccatgtta ttaggctcac acgtcagcat gagcggaaag
    38041 aaaatgctgt taggagcagc agaagaagca gccagttatg gatcttcgac ttttatgatc
    38101 tatacaggcg cccctcaaaa cactagaaga aaagccatcg aagagatgaa tattgatgcc
    38161 ggaaaagcgt acatggcaga aaaaggtcta acaaatatcg tcgttcatgc cccttatatc
    38221 attaatttgg ggaatacgat caaaccagaa atgtttcctt ttgccgtcca atttctgaga
    38281 gaagagattg ttcgtgcgga agctttaggc gctactcaaa ttacacttca cccaggtgct
    38341 catgtagggg caggaacaga agccggaatt gctagaatta ttgaaggctt gaatgaagtt
    38401 ttgacaaaag aacagacggc acaaatcgct ttggagacaa tggcaggtaa aggtacggaa
    38461 atcggtcgaa catttgagga attagcgcaa atcatcgatg gagtaaaatt gaatgaaaaa
    38521 ttatccgtta cttttgacac ctgtcatacg aatgatgccg gttataatat taaagaagac
    38581 ttttcaggtg taatggaaga gtttgataag atcatcgggt tagatcggct taaagtactc
    38641 catatcaatg attctaaaaa tccgcaaggt tcgcataaag accggcacgc caatattggt
    38701 tttggaacaa tcggattcga cacgctgaat aagatcgtcc atgatccaat gtttgacaat
    38761 atttcgaaaa tcttggaaac tccctatgta ggcaaagata aaaagaaagc ttacgcacca
    38821 tatggaaaag aaatcgccat gttcaaagcg caaacattcg atccagaagt gtttcctgag
    38881 ttattagcag aagaagtgat actctaagat tttctatctg acagcagcaa aaaaggacca
    38941 ggcacattgc cttgtccttt tttgtttaag aaacaaagga gtactttttg cagtcttttt
    39001 catcatgcat cttctccccc ctctttttaa atttagacaa acaaggtata ctggtagaaa
    39061 agtaaaaaaa cgaaaagttt cacaagcaag tggcaaaacg tgattggaag gacgaaaaga
    39121 tgctgaacac tttaaaaccc ttgatcaacc aactaaaaaa gaaacataca tactttattc
    39181 ttggcatagc cctattattt acaattattc gtttgtattt atatagtaaa gcaatctatg
    39241 gaacagacat gtcggcaacc tatgatgacc agctgctgat tcgctatgct caaaatattt
    39301 tggacggtaa atggctaggg gagtatacga ctacaacact ttccaaagga atctcctaca
    39361 gcttattttt agtacttgca cataaactgc atctttctta tgcagttttg ttcggcatgt
    39421 tcagtatttt tgccagtata ttggttgcac tcgcgtttaa accgattgtg aagaacaagt
    39481 atcttctttt aagtatctat ttttattttt tgttttctcc gattagtttt acacatgaat
    39541 attccactcg aacatatcgt aatgccctcg tgattccggc tgttgagatc attatcgctt
    39601 gtttattagc tatttattac cgcaaatacg gttctagcag aaaattgctg ccatggtttt
    39661 tagggctttc tgtcgctttt cctttctttt ggttcattcg cgaagattct atctggcttc
    39721 ttccttttac tgtagtagct ttgggtatca gtttcgtcca gatcttgcta gggcaagact
    39781 tttctttcaa agaagtaaag aagttaaaaa aacgactctt tcttctctcg aaacgggaat
    39841 ggctgaaact ttcattgttt ttacttccta tagttggctt ttccatcgtc tatcaatgga
    39901 tcgaatataa aaatgagaca acatacggta ttgcaaccat caatgaccgt tccagtggag
    39961 aatttggacg tttgatgaaa caactgatcc gaatagatga tgaaacgaac ttgaataaag
    40021 aaaacagtga gatatgggtc tcacatgctg cactaaataa agcattagcc gcatctccta
    40081 cattccatac tgtttctacc cgtattgacg aattatatac gactcacgcg tggactcaag
    40141 gtggaaaagt aaaagaatta cccggagata tcatcttttg ggcattgcga gatgtggtaa
    40201 gcgaatctgg ttattaccaa aacaatgcaa aagaaaccaa tgatttttgg aaaaaagtca
    40261 accaagaatt agaagaagcc tatcacaagg gaacaatcaa aaagaaaaaa gagttctatc
    40321 tgatgggatc aggagatgga aagacgatga gtgatctgcc aatcgtattc gatttccttc
    40381 aatcaggata cgcttatgca tggcgatata aagatttcca tcaaggagga aattatagtt
    40441 tcggaacgca agaacaagtc aaacaggctc aagaattatt aaaccttccg ctaatggaga
    40501 attggatcga tagtaatgaa ccgagaacac ctaatttcca attaacaaaa tcagcaaaag
    40561 tcgcgaatgt catcataaaa atttaccaag ttctggctat tcccgtgctg attgcttcaa
    40621 tagcagggat attacttatg atactaggaa ccgtatttgc aaaagctgat cgtgcctatt
    40681 atagttcctc tttgattgtg gtcaccggat taatactgac acaattggtt tttcttttcg
    40741 gtgtctcatg gttttgttct tatgcgccag aaaaaaagga ctatttttta agtgtctaca
    40801 ctggtgcagg cgtgccatta acgcaattca tcgtagtaac aagcatgcta gttttcagta
    40861 aattaaatct gaaaaaagct ttatcttcta aataaaaaga cccttggtag ctagattttt
    40921 aacctaacta tcaagggtct ttgtgtggaa aaatcagttt ttattcgatt tcttctaccg
    40981 tttcctgcaa aagctgttca tattttttgt ttcctttata taaatcacca atcgtccatg
    41041 ccaacaggag aaggacaaga acaatgatta cataagcgat gtgatcagcc aatgctagtt
    41101 gagcggaagt ggcttgctcg ccgaagaaac tttcgatctg tcctggtaat cctcgtaagt
    41161 tcaagtaagt tagactaatc acagagaacc agccaagaag ttttacgatg aatgaatttt
    41221 taaatcggct acccatctcc gcttggctgt tggtcatcat cagtaaagga atcatcgaaa
    41281 acggcaacgc aaaagccaag aacacctggg attcattcat taagttattc aaagcggcat
    41341 gttcttcgat cgttcctttt gtacttgtaa acaaaacaca aatcaataca ggaatcacag
    41401 aaagcaatcg tgtgaccagt cttcttagcc aaatcgggat acgcatatgg atgaatcctt
    41461 ccatgatgac ttgaccggtc aacgtaccag taatagtaga gttctggcca gaagcaagaa
    41521 gtgctacagc aaataaagtg gacaagatac cagattttgc tacagcaatc aaaataccat
    41581 tgctcatagc tgatggatca gataaagctt catacagacc aaaaaaggaa ggatcttgga
    41641 cagaaccggt tttgaagaca gcgacaccca tgatcaatag cagtgcattg actagaaaag
    41701 ccattgacaa ctggatatta gaatcccaag tagaaaaacg tacagcttgt gctacatctt
    41761 cttcgttttg gtgatcgatc ttccgagttt gtgaaactgc tgaatgaaga tataagttat
    41821 gcggcatgac tgttgcacca ataatcccta aagctccagt cagcggagtt tcgccgccga
    41881 tgaccggtga gtctgagaat gtatgactgt tagggataaa gccagcaaat aaactgtgcc
    41941 aatctgggtt agacaagcca acttgataga caaaaacgaa taaaatgacg aaaattaagc
    42001 agacaaccag tgcttcgatc ttacgaaacc cgacttttgt cagtagtaag agaacgaaaa
    42061 catctaatac agtaataaag acggcaatca ccaatggaat atcaaaaagt aagtagaggg
    42121 cgattgctgc accaatgact tctgcaatat ctgttgccat aatggctaat tcagtcaaga
    42181 tccataaaat gacacctaat gtcttacttg tgcgtgctcg gattgcctgt gccaaatcca
    42241 tttggctcac gattcctaat tttgctgcca tatactgtaa aagcatagca atcagactag
    42301 acatcaaaat aactgacatc aataaatatt ggaaattttg tcctccggtg atcgaagtag
    42361 accagttacc cgggtccata tacccaactg ctactaacgc accaggtcca gagtaagcaa
    42421 gaagtgtttt aaaaaaactt ttcccctttg gtacatcaac tgttccattt acttcagcta
    42481 aagataaacc atttgtatgt tcaataagtc gatgcttatg aaattgttta ttggattgat
    42541 tattcaagaa aaaaccacct ttcctattta tctacgatta cattctacac ctcaaaaaga
    42601 aaaaattaaa gaaaaagatt cggtacgcct aatttttttc atggcgcgtc ttgctagatg
    42661 acgaaagaaa ttttgttctc ctctaaaaaa accactattt tttgcatgaa actaaagaat
    42721 acgcctttct tttttcaata aactttagta aaataagtca ttgagagcaa ttcggaaata
    42781 gaaaggagag atttatatgt tatcaacagg tattgtcgta cttattgcta ttattgcttt
    42841 gctaatcggc gcagtaggtg gctttttcct agcgcgtaaa tatatgaaag actatttaga
    42901 aaaaaatcct cccgttaatg aggacatgtt gcgatcaatg atgatgtcaa tggggcaaaa
    42961 accatctgag aagaaaattc gccagatgat gcagcaaatg aagaaccaag ggaaaaaata
    43021 ggttctgacc caacgacttt ttacgggctg attttttcag tccgtttttt atttcgtaat
    43081 ttgttagact attcagatta ttcagcaaaa aagacaagca cagacaatcc taggctcttg
    43141 ataaagacaa aggaggagta aaatgtcgat ttttagaaaa ttaggctggt tttttcgtca
    43201 ggaaaagaaa cattatttaa ttggggtttt ctcgctattc gcagtggcct tggtacagtt
    43261 agttcctcca aaagtgattg ggatcatcat tgatgagatc gccgataaaa atttatcaat
    43321 aaagatcatt ttcgggtgga tcttcctatt gattatcgct gcttttttac aatatctttt
    43381 tcgttatatc tggcggacga atatttgggg aagtgcagct cgattggaga aagaacttcg
    43441 gcagcagtta ttcaaacatt ttacagaaat ggacaatctc ttttaccaaa aatatcggac
    43501 tggggatttg atggctcacg caacgaatga tttgaatgcg atccagaatg tagcaggagc
    43561 tggtatcttg acctttgcag attcagtgat tacgggcgga acaacgatca ttgcgatgat
    43621 tttgtttatt gattggcgct taacactgat tgcgctgatt cctctgcctt tgctggccgt
    43681 cacttcacga gtgctggggt caaaactcca tgatgctttt cgtgattcgc aagaagcttt
    43741 ttctagtatc aatgataaga cacaagaaag tatcactggg atcaaagtca ttaaaacatt
    43801 tggacaagaa aaagaagatc tagcggattt tgaagaaaag attgaagatg caattattaa
    43861 aaacaaacga gtcaattttt tagactcctt atttgatcca ttcattacct tgattatcgg
    43921 tctgtcttat gttgcaacaa ttattttagg tggaaagtat gtcttggaag gcacgatctc
    43981 gatcgggcag atggtctcat ttattagtta tatcggtatg cttgtttggc cgatgtttgc
    44041 gattggtcga ttgttcaacg tattggaaag aggaaatgct tcttatgatc gcgtcaatga
    44101 acttttgcat gaaaagacgc atatcgtgga acaactgggt gcagtgcaga ccccggcaaa
    44161 aggcgagctg aatgtagcta tttcctcttt taattatccg aaagaagaat caccagcatt
    44221 agaacaaatc aagtttgcga tccaagaagg agaaacatta ggtattgttg ggaaaactgg
    44281 tgcgggaaaa acgacgatca tcaaattact gatgcgtgaa tacgatgatt atcaagggaa
    44341 aatccaattt ggcggacgtt cgatcaaaga atattcattg aatgctttat taggatcaat
    44401 cggctatgtg cctcaggatc acttcttgtt ttcgatgacc attcgagata atatccgttt
    44461 ttctaatcca aaactttcag aagaagaagt agagcatgcg gcttctttag catttatcaa
    44521 caatgaaatc aaggaattac caaaaggtta tgatacctta gttggagaac gtggagtctc
    44581 tttatctggc ggacagaaac aaaggatttc gattgctcga gcattgatca aaaatcctga
    44641 attattaata ttagatgatg cactctctgc agtagatgcc aaaacagaag aagctatctt
    44701 gcataattta aaagaaataa gaaaagagaa aacaactatc attgcagctc accgtttaag
    44761 tagcgtgatg catgcaaaag aaatcattgt tgtcgatcaa gggaaaatca ttgaacgagg
    44821 aacccatcat gagctgcttg cactaggcgg ctggtatgaa caaatgtggg agaaacagca
    44881 attagaagcg aagatagaag gaggcgaggc ctgatggatg aaaattatca atcggaatgg
    44941 acaaaatcga ttccacttaa agaacagaaa aagatcatca aacgactgat gaagttcgcc
    45001 aaacctttta gaagaacgtt catcgttgct atcttatttg cgtttgcttt gtctgtgatc
    45061 aatgtcctat tgccaaggat catccaaact tttatggatg atcatctggc taaacagtcg
    45121 gctacgacac aagttatttt gttttttgct ggtatgtact tatttggagt catagtaaaa
    45181 agtatcattt ggtttttcca atggtatttg tattccatgg cctcattgaa gacctatcag
    45241 tatattaggg taaaattatt tgaaaagctg catacactag gtatgcgtta ttttgatcag
    45301 acaccggcag gatcaaccgt ttctcgagtg acgaatgaca cagaaacgct atttgaattt
    45361 tggtacgtct ttttgatggt attgactggg atctttgcag tcgtttcttc attccttgcc
    45421 atgtttcaaa tcaatggaga aattgccttg tggactttgg tgtttttgcc gattttagga
    45481 atcgtcatct ggtattacca gaaattcagt tctcgtattt accgaaatat gcgggaaaaa
    45541 ttaagtcagc tgaacacgaa attgaatgaa tcgatttccg gcatgcgtat tatccagcag
    45601 tttcgccaag aagctcgttt agctaaagaa tttgaacaag tgaacaatga gtatttagac
    45661 acgcgttatg cgatgatcaa aacgaattcg attctgttaa gtccgatcat caattttcta
    45721 tatgcattgg cgattgcgtt ggtgttgact ttgtttggga tcgatgcgtt gcattctccc
    45781 gtagaagttg gactgatcta tgcatttacg acctatgtcc agggattttt taatccaatg
    45841 tctcaaatga tggacttttt gagcatcttc acagatggga tggttgctgg tagtcgtatc
    45901 ttgaagatta tggatacgga agaatatgca ccacaacaaa accctggagc agatcaaatg
    45961 attacttcag gtaagactga gtttcgcaat gtaagctttt cttatgatgg agagcacaat
    46021 gtattaaaca atatctcttt tgttgctaat ccgggagaga ccgttgcatt agtgggacac
    46081 accggaagcg gaaaaagctc gatcatcaac gtattgatgc ggttttacga attttatgaa
    46141 ggtcagatat tgattgatga tcatgacatt cgagaatatc caatggaaga gttacgtaag
    46201 aaaatggggc tggtcttgca agatgctttc ttgttttatg gggatatcgc aggaaatatt
    46261 cggatgatga atccagctat cacagacgaa cagatacgcc atgcggctga gtttgttcaa
    46321 gcaaacaaat tcatcgatga attaccaaaa ggatatcact caaaagtgat cgaacatggt
    46381 gccagctatt ctagtggaca acgacagctg atcacttttg cgcgaacaat cgtgactgat
    46441 cctaaaattc tagtattgga tgaagcaaca gcaaatatcg atactgaaac agaaggattg
    46501 atccaagaag gattggcaaa aatgcgtcaa ggacggacaa cgattgcgat tgcccatcgg
    46561 ctgtcaacga ttcgagatgc agaattgatt ttagttttag acaaaggtca tatagtagaa
    46621 cgcgggaatc atgaaacact tctagcgcaa aatggtttgt atgcagatat gtacaaactg
    46681 caaagcagtg gtatataaaa gatgttgtga cagtagaatt ttgacctaag cactagagtg
    46741 aaaataaaac atagcttctc atatttttca tctgtgagag ttagtgtcgt agtcaccaga
    46801 tttaaaaaaa gattcgacag ctagtcagaa gtgcctaata gccactaaga aagcgttgac
    46861 aaaagcattt gctttcgata atataaagaa aacgatacaa cttggatagt gattgcctga
    46921 tgcaagc
//
LOCUS       DAZUMK010000010        45054 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000010 DAZUMK010000000
VERSION     DAZUMK010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45054
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.010"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..919
                     /locus_tag="ACG808_000562"
     CDS             <1..919
                     /locus_tag="ACG808_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFS5046215.1"
                     /translation="SKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILI
                     LVSKPFFLSYKGDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFL
                     YSYGYIKMSGLKMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQV
                     AFLRIDQTAPWLFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVV
                     FVPALNIVILAVLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNA
                     SLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
     gene            938..1957
                     /locus_tag="ACG808_000563"
     CDS             938..1957
                     /locus_tag="ACG808_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFS5046216.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(2059..2775)
                     /locus_tag="ACG808_000564"
     CDS             complement(2059..2775)
                     /locus_tag="ACG808_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS5046217.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(2744..3529)
                     /locus_tag="ACG808_000565"
     CDS             complement(2744..3529)
                     /locus_tag="ACG808_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5046218.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(3520..4269)
                     /locus_tag="ACG808_000566"
     CDS             complement(3520..4269)
                     /locus_tag="ACG808_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFS5046219.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            4567..5091
                     /locus_tag="ACG808_000567"
     CDS             4567..5091
                     /locus_tag="ACG808_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFS5046220.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            5107..6606
                     /locus_tag="ACG808_000568"
     CDS             5107..6606
                     /locus_tag="ACG808_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFS5046221.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(6694..7296)
                     /locus_tag="ACG808_000569"
     CDS             complement(6694..7296)
                     /locus_tag="ACG808_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS5046222.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            7400..8356
                     /locus_tag="ACG808_000570"
     CDS             7400..8356
                     /locus_tag="ACG808_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFS5046223.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(8661..9617)
                     /locus_tag="ACG808_000571"
     CDS             complement(8661..9617)
                     /locus_tag="ACG808_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS5046224.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            9791..10351
                     /locus_tag="ACG808_000572"
     CDS             9791..10351
                     /locus_tag="ACG808_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046225.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            10344..11168
                     /locus_tag="ACG808_000573"
     CDS             10344..11168
                     /locus_tag="ACG808_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFS5046226.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            11178..11795
                     /locus_tag="ACG808_000574"
     CDS             11178..11795
                     /locus_tag="ACG808_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFS5046227.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            11813..12496
                     /locus_tag="ACG808_000575"
     CDS             11813..12496
                     /locus_tag="ACG808_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFS5046228.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            13179..14462
                     /locus_tag="ACG808_000576"
     CDS             13179..14462
                     /locus_tag="ACG808_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5046229.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            14478..15365
                     /locus_tag="ACG808_000577"
     CDS             14478..15365
                     /locus_tag="ACG808_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5046230.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            15382..16218
                     /locus_tag="ACG808_000578"
     CDS             15382..16218
                     /locus_tag="ACG808_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5046231.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            16221..16844
                     /locus_tag="ACG808_000579"
     CDS             16221..16844
                     /locus_tag="ACG808_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046232.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            16841..18133
                     /locus_tag="ACG808_000580"
     CDS             16841..18133
                     /locus_tag="ACG808_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS5046233.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      18492..18589
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            18705..19286
                     /locus_tag="ACG808_000581"
     CDS             18705..19286
                     /locus_tag="ACG808_000581"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFS5046234.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            19289..20596
                     /locus_tag="ACG808_000582"
     CDS             19289..20596
                     /locus_tag="ACG808_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFS5046235.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            21012..21500
                     /gene="purE"
                     /locus_tag="ACG808_000583"
     CDS             21012..21500
                     /gene="purE"
                     /locus_tag="ACG808_000583"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFS5046236.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            21493..22617
                     /gene="purK"
                     /locus_tag="ACG808_000584"
     CDS             21493..22617
                     /gene="purK"
                     /locus_tag="ACG808_000584"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFS5046237.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            22630..23925
                     /gene="purB"
                     /locus_tag="ACG808_000585"
     CDS             22630..23925
                     /gene="purB"
                     /locus_tag="ACG808_000585"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFS5046238.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(24105..24482)
                     /locus_tag="ACG808_000586"
     CDS             complement(24105..24482)
                     /locus_tag="ACG808_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046239.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            24738..25466
                     /gene="purC"
                     /locus_tag="ACG808_000587"
     CDS             24738..25466
                     /gene="purC"
                     /locus_tag="ACG808_000587"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFS5046240.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            25466..25720
                     /gene="purS"
                     /locus_tag="ACG808_000588"
     CDS             25466..25720
                     /gene="purS"
                     /locus_tag="ACG808_000588"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFS5046241.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            25722..26393
                     /gene="purQ"
                     /locus_tag="ACG808_000589"
     CDS             25722..26393
                     /gene="purQ"
                     /locus_tag="ACG808_000589"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFS5046242.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            26394..28616
                     /gene="purL"
                     /locus_tag="ACG808_000590"
     CDS             26394..28616
                     /gene="purL"
                     /locus_tag="ACG808_000590"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFS5046243.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            28601..30040
                     /gene="purF"
                     /locus_tag="ACG808_000591"
     CDS             28601..30040
                     /gene="purF"
                     /locus_tag="ACG808_000591"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFS5046244.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            30063..31115
                     /gene="purM"
                     /locus_tag="ACG808_000592"
     CDS             30063..31115
                     /gene="purM"
                     /locus_tag="ACG808_000592"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFS5046245.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            31112..31690
                     /gene="purN"
                     /locus_tag="ACG808_000593"
     CDS             31112..31690
                     /gene="purN"
                     /locus_tag="ACG808_000593"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFS5046246.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            31687..33222
                     /gene="purH"
                     /locus_tag="ACG808_000594"
     CDS             31687..33222
                     /gene="purH"
                     /locus_tag="ACG808_000594"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFS5046247.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            33238..34512
                     /gene="purD"
                     /locus_tag="ACG808_000595"
     CDS             33238..34512
                     /gene="purD"
                     /locus_tag="ACG808_000595"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFS5046248.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            34634..35548
                     /locus_tag="ACG808_000596"
     CDS             34634..35548
                     /locus_tag="ACG808_000596"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFS5046249.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(35681..37246)
                     /locus_tag="ACG808_000597"
     CDS             complement(35681..37246)
                     /locus_tag="ACG808_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5046250.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(37239..38690)
                     /locus_tag="ACG808_000598"
     CDS             complement(37239..38690)
                     /locus_tag="ACG808_000598"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS5046251.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIRESKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(38704..39720)
                     /locus_tag="ACG808_000599"
     CDS             complement(38704..39720)
                     /locus_tag="ACG808_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5046252.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            39940..41028
                     /gene="chvE"
                     /locus_tag="ACG808_000600"
     CDS             39940..41028
                     /gene="chvE"
                     /locus_tag="ACG808_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS5046253.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            41082..42608
                     /gene="mmsA"
                     /locus_tag="ACG808_000601"
     CDS             41082..42608
                     /gene="mmsA"
                     /locus_tag="ACG808_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS5046254.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            42610..43815
                     /gene="mmsB"
                     /locus_tag="ACG808_000602"
     CDS             42610..43815
                     /gene="mmsB"
                     /locus_tag="ACG808_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFS5046255.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            44073..45050
                     /gene="guaC"
                     /locus_tag="ACG808_000603"
     CDS             44073..45050
                     /gene="guaC"
                     /locus_tag="ACG808_000603"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFS5046256.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
BASE COUNT        14271 a         7772 c         9970 g        13041 t
ORIGIN      
        1 atcgaaagtc gtcttaacag tggctttgcc agcactggca tttcgagctt tcatgcaaga
       61 tatcaataca gaattactga tgcaaggaat caatgtcttg atttggggat tagttattta
      121 tattattctt attctagttt caaaaccttt tttcttaagc tataaagggg ataagcaaac
      181 aacgttgcgt gtcctgacga tctttggttc tacgaccttt ttcggaacgc ctatcgtcag
      241 tgcgatttat ggtcctgtcg gcgtgatgta ttcctcaatt ttcaatattg gctaccgaat
      301 ctttttgtat tcttatggat acataaaaat gagtggcttg aagatggaag ctaaaaatat
      361 caaagcgatg ttcgtcaatc caatcattat cgctactttt gccggccttt tcatttggct
      421 gttccaaggg tatctgccac aagtgtcagt tacaggtgca gaaggagaga taagccaagt
      481 agcgtttttg cgaattgacc agactgcacc gtggctattt cagccgatgg attatttagc
      541 tggtttaagt tcaccattgg cgtggctagc aatcggcgca acattaggtg aagtaagctt
      601 caaaaaagct gcatctgaaa aaacttcatg gtattacagt ctagtcaaag tggtatttgt
      661 acctgcattg aatatcgtga ttctagcagt tttgacggca acgaatattc taccagtcaa
      721 ttttgaagca ctggcaacaa tcgttatcat gatggctaca ccagctgcaa cagtggcagc
      781 agcctatgct atcagttttg atagggaggc acttttagcc tccaatgcat ccttactgtc
      841 aactattttt gctgtggtac tgacacctgt atggatcgtt gtattacaaa tgattcatgg
      901 cataggttta ttttcataag aaatggagga agaaagtatg agaatcgcat gttacggtgt
      961 tcgtccgaac gagatagatt tttttgaaaa attgaatatt taccattatg atctcagtct
     1021 ttatgaagaa ctgttgacac atgacaatat cgtgtcagca aaggaccatg atgctgtatt
     1081 gttgagggga aattgtatag cagatgagac caatcttgca aaaatgcagg aatatgggat
     1141 tcgttatgtt tttacacgga ctgtgggagt caatcatatc gatctacaag cggcagctga
     1201 ttttggtatg acagtagccc gtgtaccatc gtattcgcca aatgcgatag cagagttatc
     1261 tttgactttt gccatgatgc ttttgagaaa tactgcgtat acgacgatcc gaacatcttt
     1321 caaagatttt cgagtggatg agcaaatgtt tagtcgtgaa atcagaaatt gcacagtagg
     1381 gatcatagga acaggcagga tagggttgac ggaagctgtt ttgtttaagg gactaggagc
     1441 caaagtgatc ggctacgata ttcatcagac agaagctgct aaaaaagtat tgacgttcca
     1501 ctcgcttgaa gagttattag ccaaaagcga tattgtcagt attcatgtac ctcatatccc
     1561 aggtgaaaac gatcaaatga tcaatgaagg atttttagct caaatgaaaa aaggtagcat
     1621 cttgattaat acagcccgtg gagagcttca agataatgaa gctattttaa gagctttgaa
     1681 aagcaatcat ttagctggtt ttgcaaccga tgtttttgca aatgaaacag aggttttttt
     1741 ccgatccttc aagccatggg agacgattcc agaccctgct atccagcaat tagtagaaat
     1801 gtaccctaga gttcttgtaa ccccgcatgt aggttcaaat acagatgagg cattatcaaa
     1861 tatgatttcg acaagtttcg agaactttcg tgaaataata gaaactggaa aaacgaaaaa
     1921 tgaggtatct cttcctaaag cccgtcaatt aagataagaa aacatggata ttcgtcaata
     1981 aaatgtttaa aaatagtcca agtaaacagt tgttacttct gtatacttgg actattttgt
     2041 atgggataat tcgatgactc aacagtaatc tgtgagcttt ttccgcccat tctttgtcaa
     2101 ttcataacag gttagcggcc ggccgatttc caaatatttg attctttcgt tcaatatatc
     2161 ttgttctact aaaaaattca aatatttctt gacggatata cgtgacagtg agcttttttg
     2221 agccagttct tttgttgaaa aaatagtatc ttcttttaga ataaatgcac ttattttttt
     2281 taaagtcagt tttgacagac ctttaggcaa atcgatgaat gatggtattt cgtgaatggg
     2341 agtggtgtgg ttgaatactt gatcgatcaa tttctgatcg gctttttcag tcgtttggat
     2401 tgtctgagag aaagcaagat acttttcgat cgccagctga aaacgttgca aagtgaacgg
     2461 tttgatcaaa taatctaagg caccgtagcg gatcacattc tttatggttt cgatatcatc
     2521 tgcagcagtg atcaaaatca ctgggatatc atttccttgc tgtctaagcc aagataaaaa
     2581 atggatacca ctttcgccag gcatgtatac atcaagcagc agcagatctg cgtttttttg
     2641 tctgaaatat ttttttcctt cttctacatt tctgaaactt tcgattacgt tcatattagg
     2701 gatgctttcc acaaaagtac gattcaattc tgcaaccatt ggatcatctt cgattatcag
     2761 gacatttgtc atcattttga tcttcctttt ttggtaattc tagatagacg attgtaccgc
     2821 catttgtttg tttggaaaat tctaaaaatc caccgtggcg tttgacgatt gaatggatga
     2881 gattcaatcc aaacccacga ttgattccct tcgtcgaaaa acctagcgta aatatttttt
     2941 ctgagatttc ttctgatatc ccgacaccgt catcgcagac ggtcacgatg aatacttcag
     3001 cttctgtgtc atagtttaaa tgaatccgga cctgtccgtt tctttcatgt ttttgtagca
     3061 cggcttcttt tgcattatca agtaagttcc ctaacacttg caaaagtgaa tggatggagt
     3121 catccatctc cattttaggg ataaaagaat cgctttctaa cagcatttca acagattgtt
     3181 ctttggcttc gtttattttg cctaataaaa aaccggcaat tgcaggtact ttgatcagac
     3241 ttgagacttg gccaatctct tttttataat tttgatttag ctgctggata aaatgggtga
     3301 cttcttcata cttcttcaaa tcaatcaaac cagaaatgac atgaagttta ttcatgaatt
     3361 catgggtttg agctctcatg gagtttatat attgtttcgt ccccccaagc tcctccatta
     3421 atttcattac ttctgatcga tttcgtaaag ttgcgactgc tcctaaaaaa cggtcattgc
     3481 taaaaagagg tgtaacgcta gaaatagttt ctatcccatt taaatacatg gattgagtcc
     3541 gaacggtcgt ttgatgctca atcgtctctt taaacaaaac gtcatataga ttatcactga
     3601 tttttttcct acttcgaaat ccaatagctg ctctgtttgg tagaaaatgt tttttgcagg
     3661 tgtattgatc aagaggatag ttttatcaga agagatggct aagattcctt catctacttc
     3721 attttcgata atcgtttttt cacttaactg ttctgctatt tcttgtggtt caagtccaag
     3781 cagtactttt ttcatttttt gggcaagaaa aacggcgcct atgatgccta cgctaagtcc
     3841 aataatcaat gaaaaaacaa tcattttttg tccatctttg acttgattgt caatcgtgtt
     3901 gtttgttaag ccaacacaaa ctgcaccaat aatctcttga tcataataaa tagggacgaa
     3961 atagcggaag ccgaacccta acattccttc tttttcagag aaaaattctt gtcctccaat
     4021 cacttttttc acatcttcat gacttgagaa aggaagaccg atgtaacttg ggtctgcgtg
     4081 agaatatcga atgccttcag ggtccatgac aacgataaaa tctaaattcg atgcaatcat
     4141 gatttcattt gttattcctt gaatcgcatg tgtcgggtca ccagtctgca gctttttttg
     4201 gatgagttta tcattggcaa cgattttagc aatgctagag attttatctg acacactaga
     4261 tcggttcatc gaaagaataa aattcctgat caatatggca gaaacaatca aagaaacgaa
     4321 tgtggagaaa caaacgacaa ggatgatcgt gttctggagc tttaatggtt ttttcttaaa
     4381 aaaaggaaac ataggctacc tcgaaagaat ttattgtgaa aattttaaca tgaaaacaaa
     4441 aatttaacaa attgtcaacg ataaacacaa ccttcctata aaaaacacca ctgagtcggt
     4501 aaaagaagct atacagaatt ttcaaacatt ggtatgatag acgtagaaat ggaaggaggc
     4561 gtgtatatga tagggggaat cgcacttgag acgatttatt ggtatacatt gctgatttgt
     4621 gcaggattgg cagtcctatt gattattttt ggagatgtat tcgattttga tggcccgctt
     4681 gatccgatgc ttattatccc ttggttaact ttcacaagct tatttggttt tctaggtgag
     4741 cgtttaacag atcaatcatc tttattttta tttattgcta gtggaggcat atcgatagtg
     4801 ttggtatttt tgcttaattt ctatatattg atgccgatga aacatgccga atcaacgatg
     4861 tcgtcttctg aaaaaactct ggaaggacaa gtagctgttg tgatcacacc gatccctgta
     4921 cgaggtatgg gagaaattca gttgaaaagt gttaccggtt ccattagtcg tccagcttgt
     4981 ttttacagcc cccaagaaca gacagctgag cggggaacga aagtattgat cattgagatc
     5041 aaggaaagaa tctgttatgt catgccttat gaaagcatga taaaagctta aaggagcgtg
     5101 tgttaaatgg aaataccagt tgtttttatc gcgatcgcaa tcgcagcttt gattttactg
     5161 atgttgctga tcgtctttgt agcgaagtac cagacagcaa aaccggatga agcgttgatc
     5221 atcagtggaa gttatttagg aagcaaaaat gtgcacattg atgaaagcaa taacaaaatc
     5281 aaaattgtgc gtggaggtgg cgcattcgtc ttgccagttt ttcaacgttc gaatcggatc
     5341 agtctattat cgagtaaatt ggacgtctcc acccctgaag tgtatacgga acaaggcgta
     5401 ccggtgatgt gtgatggtac ttcgatcatc aaaatcggtt catctgtaga agagattgct
     5461 acagcagcag aacagttttt aggcaagaca agagaagaac tggaaaatga agcaagagaa
     5521 gtattagaag gacacttgcg ctcgattctt ggttctatga ctgtagaaga aatctatcaa
     5581 aatcgcgata agttcagtca aagcgtacaa gaagtcgctt ctgtagacct tgcaaaaatg
     5641 gggttgatca tcgtttcttt cactatcaaa gaggtcagag acaaaaacgg ttatcttgac
     5701 tctttaggga aaccaagaat cgcccaagtc aaaagagatg cagatatagc agaagcggaa
     5761 gctctaaaag aaacgcgtat caaaaaagct caagcagaaa aagaatcaca agcagctgaa
     5821 ttacagcgac aaacagagat cgcagaatca ttaaaagaaa aagaactgaa attagcaacc
     5881 tacaaacaag aacaagatgt tgctaaagca aaagcagacc aagcctacaa tttagaaagt
     5941 gcccgagcac aacaacaagt aatcgaacag gaaatgcaga ttaaaatcgt agaacgtcaa
     6001 aaacaaatcg aactggaaga aaaagaaatc acacgtcgtg aaaagcaata tgattcggaa
     6061 gtgaagaaaa aagcagatgc cgatcggtac gctaaagaac aagaagcaca agctcaaaaa
     6121 gtcaaagaag tgactgaagc cgaagcagaa cgattccgtg tggaagcact ggcagaagcg
     6181 gaagccaata agacacgttt agctggacaa gcagaagccg aagcagcact agcaaaagga
     6241 aaagcagaag ccgaagcaaa acaaaagatc gccaatgcct tcaaggaata tggcgaagca
     6301 gctgtcttga gtatggttat cgatatgcta ccgcaattga tgcgcgaagc agcacagccg
     6361 ctaggaaata tcgaaaaaat ctctgttgtc gatacaggaa gcagttcagg agaaaccagt
     6421 ggagcgaacc gagtgacaaa ttatgccaca aatttgcttt cttctactca agaaacacta
     6481 aaagcgacaa caggacttga cttgaaggaa atcgtagaga atttgtcgac acgtgggact
     6541 actagccaag taaatatgta tgaagcgcca aaagaggcag aacaaaactc gatgaaggaa
     6601 gaataaagta gtacacttca ccgttcaatc aaaaatggag gaagactttt taaagttctt
     6661 ctctccattt ttttatttta ttgcaaaaga aaattagtcc tcgctatttt tcttttgttt
     6721 ttggatctta gcttctatct cttgtaaatt tgttcttagc aatcgttctt tcttttggag
     6781 ttcgaccaaa tcttctgata gttcttcctt cttttctgtt tcaggaggac gaagaaagaa
     6841 attgataccg attgcaatga atgtccctat aaaagtatct agtacacgtt ggatagcata
     6901 caaagcggat tcgccttgag ggatgctcaa cgcaatcagc aacaacgtag ctattgcgga
     6961 aatgataccg gaattattgt taataccatc tgacacgacg atcacaataa taactagaat
     7021 aggaagcaag atcaattcta ccaaaaaatc attgtgaaaa tagcttttga caaaaaaata
     7081 aatgatagct aatgtaccac ctaatgtatt gccgatgatc ctagactttc caaaagaaac
     7141 ggttgtagtc aagtcctgac gcaaagaaaa aacagcagat aatgcagcaa tcatcgggat
     7201 tcctcgttga gtcaaatgaa aaaacaagat acataaaaaa acagctaagg cacttttgat
     7261 cgtacgcata cctagtcgaa aacggccaaa ttgcatagag attctccttt ttggtctttt
     7321 tactatcata cctaaacttt tgtctgatag ctagtttttc attattcaag tataattaaa
     7381 ataaaaaagg gggaagtaaa tgcgtatagg aatgcgaaca gtcaaaacag tgatcagtgt
     7441 cttcttttcg atggtcacag cgggtagctt atcgctgttg tattggccag ctgcagggat
     7501 cattgcatta ttaagtgtag gaaataccaa gcgctctaca ttgcgtacgg gcatgaatcg
     7561 aatcgcagcc tttatattag ctacggcaac agcactgttc agtttttttg ctgttgggta
     7621 tactattttt ggcttcagtc tttttttatt cctgttcatc ccgcttgcct ctcgcttcaa
     7681 attaaccgaa gggatagtag taaattcggt tttggtcact cattatttag tggaggaaaa
     7741 catgtcttgg cagctgatcg gcaatgaagc gggattaatg gcgattggat tagtatttgc
     7801 tttgcttgca aatgtttaca tgccagatcg tacgaagcag ctgaaggaaa accaaataca
     7861 gatcgaaaaa gaatttcgaa atattttgag acaaatggca gaatttcttt tatcagaaaa
     7921 taaagaagat gtgcagatca aatgcgaaca tttactgaca ttcattcgcg aaagccaaga
     7981 agatgccaga gaacatcaag aaaactactg gctcagacag ccgctatact acgaaactta
     8041 cttctctatg agaagagcac aggccaatgt gattaaagac atgctggaaa atctagagag
     8101 aatccagcaa ccagcttatt atggaaaaca tatctatggt ttattgattt atactgcaga
     8161 aacattttca gagtcaaatg atggcagaca aattttaaca cgtattgaag aagtttatgt
     8221 attgtatcgg caaatgcctc tgccgactag tcgccctgaa ttcgaggatc gagcagaatt
     8281 gttccagttt ttgcagtctt ttaaaagttt tattgaaatc aaggcagaat tctcccaaca
     8341 aaaaatccaa aaatagaggt tgtgaattca gctaatattc aggtcaaaaa gctgatttca
     8401 caacctcttc tttaaacggt gttctcctgt caactcctcg gtcacaagtc acaatattca
     8461 acaaacatga gaagctgttt tttgaaattg ctccttgtga ctcggagctg aacggtaggc
     8521 tcacaacctc tttcttaaac ggtgttcaaa agctttcctc tgcggtaata agtccagcca
     8581 aacgaaaaat aggaggagct atttttgttt ggctgttctt attacttgat gcagaacagc
     8641 tttttcacaa ccttttaaaa ttacatattt ttccatagaa cttgcatttg atccaagatt
     8701 tcgatgacat cttttgtcaa gaagagagag cgattgggca agtcaccttc aatcatgtgt
     8761 accatattct ttatttcgta attcattgct tcagatgtat tcccgctttc gatgaattcc
     8821 tttgtaccat ctgtaaataa gatctccgca cgatcagcac gcgggtaatc agcaatcgtc
     8881 acataaccgt tctcaaaggc aacgataccg acttttggca tttttgcctg aaaagtcaag
     8941 ctgacagcag caagttcatt ttctttgtta cgcaagatag tgacggattg ttcgtccacg
     9001 cctgtttcaa aaggaaccat tgtagaggca actacttcag gttgagagct caaaaagaaa
     9061 cgagcgaaag atacagcata cgtaccgata tccaacagag ctcctccagc taattctgga
     9121 ttgaagaacc ggtttttagg gtctggttct ttgtaactgc cgaaaggtgc ttggatcatt
     9181 tttaaagccc ccagttttcc tgtatccatt attgagcgta actgctgata aagtggcatg
     9241 ttgaagattg tcatcgcttc tgctaagatc aaattctttt cttctgctaa gcgcatagcg
     9301 tcagccagtt cttttttatt catcgtgatg gctttttcac atagaacgtg tttgcctgct
     9361 tccaatgctg ccaagatatg gtcgatatgc tgtctatttg gtacagcaat gtacacgatg
     9421 tcgatttcag catcgtctaa catttcttga tagcttccgt aagcttttgg aatattgtaa
     9481 cggtgagcga agttttccgc tttttctgaa gttctggaag caactgctgt tagttcgctc
     9541 gtctcttgat tgaagctttc ggcaaactca tgtgcgatgg taccaagacc aacgattccc
     9601 cattgatatt ttttcatctg aaaacgctcc ttcatagttg gattgagttt cttgtctgtc
     9661 tattattata acaaaaatca cgaagaataa agaggagagg acttgaaaca ttaaaaaaaa
     9721 tgaaagcagc gatttaagag gtgctttttg atcaaaactt aagtagaata gttaaaagga
     9781 ggggataaac gtgcatcaag tatatattga tcttattatg agtgtattat tagaacagtt
     9841 tgaaactgaa aaaaattttt tagatgaata cttgctgatc agtccagaac aatggcaaaa
     9901 atggaaagat ggcgagaaaa atctgccatc tgaagcgatg cagaaagtaa aaaatctttt
     9961 cactgattat gaatggatgc taacgcaaaa gattttgcgt caaacgatta tttttcccga
    10021 aaaacgaaac gttgccgttg cagagtacaa gcaaatcaaa acacgcatcg cgcaaaaatg
    10081 gcttagcagc aaagcagcaa aagtcgagct gatccctttg aaaaataatc agcaggctgg
    10141 tagatacctt gacttgaaag taagtattca atatgatgaa tggggctatg atgatatttt
    10201 gaattttcgt ctgccagcag atgttcaaaa acagatcgaa ggagaaaaaa tcgaacttct
    10261 cgattgggtg aatgaaaatc tggaagaaac ttacgttcat gaggcgaatg gggaacgtga
    10321 agaacaacga aaggattttt caagtgaata aatttttagg atttggagca gttgtgatta
    10381 tgatcgcagc aatgggctat gttattttta gcgatgatga ttcgcatgtt caagccggca
    10441 atcaagcagt cgaacgaaca agcaccaaaa caactgctag taaagaaaag aacagtcaaa
    10501 caaccagcag caaggtaaag gacaaagaaa caaaagatcg tacgtccaaa caattgccag
    10561 atgttaaaac agacgattgg aagctagttt tggttggtcc agatcacaaa ttagaaaaag
    10621 aaattgatga gtctactcaa ttggcatcat taagtaacgg atacttgatc gacaaacgca
    10681 ttgctccaga atatgaagca tttgccaagg ctgctgaagc agctggattt ccattagtga
    10741 tggtctcagc ttaccggtct gtatcatccc aacaacaagt tttcgaacaa aatgtccaag
    10801 atgtgatgag tcgtcaaggt gtatcagaag aggaggcgac aaagattact aaagaaacga
    10861 ttacggagcc aggatacagc gaacaccaca caggtctggc agtcgatgtc gtcgatcaaa
    10921 actggtacaa cagttatcct tctactgtat tggatgcaag ttacggagat cagcctggag
    10981 ctaaatggat cgcagataat gctgcgcagt atggatttat cgttcgttat ccaaaaggac
    11041 acgaagatat aactaagatc acatatgaac cttggcactt acgttatgtc ggtaaagaaa
    11101 acgctgagta tatcaccaaa caccaattga cgctagaaga atatctgaat gaattaaatg
    11161 aaaagtagga attgatcatg gcgaaaaaaa agtggaaaaa gcaaaagcgc aagaatatcc
    11221 gttggccagc agtggttgcc ggtgtagcag tgatactctt agcctttgtt ttttccttga
    11281 agaatctgtc ttctcctttt actgataatc agcaagaaaa tgaagcgcaa tcccatcagc
    11341 aatttattga acggcttgtt ccgcatgccc aagaactaca agatggttat ggagtgcttc
    11401 ctagtatcat tttaggacaa gctatattag aatcaaattg gggaaaaagt accttggcta
    11461 gcaaatacaa taatcttttc gggatcaagg cttatgggga tcagaagaaa gtaagcctgg
    11521 aaacaaagga atttgttaac gaagaatgga taactatcca aggagacttc aaagtgtatg
    11581 attcatggga acagtccatg gatgaccata cacaactgtt cgtccaagga gtagattgga
    11641 atcctgcgct gtatgaaaaa gtgattacgg ccaccaacta tcaggaagcc gcacaagcat
    11701 tgcaagatgc aggttatgcg acagaccccg gttatgcgca aaaaatcatc caggtcatcg
    11761 agacgtatca attgaatcag tatgaccgga gatagcaaag gagaagtggc tgatggcaga
    11821 acaatttacg ccagaaatca tgacggaatt gagaaaagat atcgtaagag taccggaaat
    11881 cattttagaa gcaagcggga tcaagatatt tggaaaattg atccgttcga tcatctttac
    11941 tacggatatt gcgatcatac gtaatacaaa tgcgaatgca gtaattgctg tgtatccttt
    12001 tacacctcat ccagcgatta ctaaaagcat tattgaggca gctgacattc cagttttctc
    12061 cggtgtaggt ggtggtttga cacaaggtgc tcgttcttcc tacatgagca tgttcgcaga
    12121 agcgcaaggt tcgttagggg tcgttttgaa cggtcctacg cctttagaga cagtaaaaga
    12181 agtttgtcga gtagttgata ttccagttat cagtacggta acgtcgaaat atacagaaat
    12241 agatgaaaag ttagcagctg gtatttcgat tatcaatatc agtgcaggta aagaaactgc
    12301 agaagcagta cgccattttc gtaaaaaata tcctgaactt cctatcattg cgacaggtgg
    12361 tccaacggaa gaaagtattg ctgagaccat tcaagcagga gcaaatgcca tcacatatac
    12421 gccaccaagt agcggagagc tttttagtcg taaaatggaa aaatatcggg aaactgagaa
    12481 aaagaatcat gaataacagg tgagaatcaa aagacgagga agagcagata gtggctcttc
    12541 ctcgtctttt gtactccagt actatttgtt cagaattcaa aaaaaagaga aatttttttg
    12601 tttttttacc tgtaatgtac ataagaagag ttaaaacctt ttgttattat tagcattcta
    12661 acaaaaaaat gtcttgattt attgaagcgc tttctataga atgaaaaacg aataaaaact
    12721 aaaaaaggca cccgacaatt tagtagcagc taaattgtcg gggcttgaaa agtcagcggg
    12781 aactgaccaa tcaagttgcc atagtcaatg cttgtgcatc gacgtgtata ttgtacctaa
    12841 tttcatacag aattactagt tatttttctg ttttttaggg aagagcgcac acgaagaagc
    12901 atgcttattt agctatgtca aggatattgg gcagttgtct gatatccttt tttctttgcg
    12961 taattatcct cccatcattg aagcaagaag tgtttttagc gatgaaagaa gaatcagcac
    13021 tatctaattt ttcttgaaag tattgagttg ttcatcctag gcagaacgat aaggcaagtc
    13081 ttggttttta ttccccttga aaaagatcgt ttaataaata tgttctgtat aaaaatacag
    13141 tatattgaaa aataacaatg gaagtagagg tgcagagaat gaagaaaaga atgagaatca
    13201 tcttagcttt aagtgttatg ttattcttct tgacggcttg tgggcaagaa gatgacaaaa
    13261 aaatcacgct gaacatggct tggtggggtt cgcaagtccg acacgatgca acagtaaaag
    13321 taatcgagct ttatgaagaa aaaaatcccc atgtgacgat cgattatgaa ttttatgatt
    13381 atgaaggata ttacacaaaa ttaggtacac tagcagcatc agatgatatc tgggacattt
    13441 tccaactgaa caatcaattt cctcaataca tcaatcagat tgaaccatta gatgaatatg
    13501 tggagcaagg gataattgat atttctaatg cagatgaatc ctatttagca acgacgactt
    13561 acgacgggca attagttgga ctgtctaacg gggtgaattc gtttgcgatt gcttacaata
    13621 aaaatatatt tgaacaatta aatatccctg aaccagcttc aaattggaca tgggatgaat
    13681 ttgaacaaat cagctatcaa atcactgaag aattaggaat ctatgctatt tcaagattcg
    13741 aagattttat agctggctgt attattcaaa taccacaagt tgagaaagga ttgaattttt
    13801 ataatcggga agatctatct aacagcttgg gatttgaaaa tccagactat ctaacagatt
    13861 ttttccagct gcgtaaaaat ctggtagaca aaggggccca tccagaacca ggtggtggag
    13921 ctgcaagtac gacggatctg tcttttcagg cggttaggga tagagaagcg gcaatggtgt
    13981 ttctatcaag taaccagttg acagaaatat tgtcaggggc tcctgaagat atggagattc
    14041 agttgattaa tccacctcgc agaaaagcag atggagaatc tggtatccct ttacgttctt
    14101 cgcaaatgct gagtatggca aaaagctcag cccataaaga agaagctgct aagtttattg
    14161 atttctttca aaacagtgaa gaagccaatg aaattttaaa aggagaacgt ggtgtaccaa
    14221 tcatgtcgaa tattcgagaa cagctcgctc gtgcagatga tgcaactttg caagcgacat
    14281 ttgattattt agaaatgatt tccgatatgg acaacggcga agtgaatgtc tttgatagtg
    14341 ctttgaatcc ggaaattcaa gaccaataca atcttttagt tgaaaaagtc atttatgaag
    14401 aagtaacagc agaagaagca gcagagagtc tttatgagtt tgcgtcaaac attctaggat
    14461 agcagcagga ggaaggcatg aaaaaagaaa aactaaaacg gttcttggat caagacaatg
    14521 tggtcggtta cgtatttgct tctccattca tcctaggatt tctattgata acagtgatac
    14581 cgatgatctt gtcactttat tattcgttca ccgattatcg tttgggaaat ccagtctcat
    14641 ggatcggctt ggataattat ataaagatgc tgagtgatgc ccgttttttg aactccatcc
    14701 gagtcacttt tctctatgta tttgcatctg tacctgtgaa actgatcttt ggtctattga
    14761 ttgcttatct tttaacgagg aatatcaaag gaacgacttt ctttcgttct ttgtattatg
    14821 tgcctagttt gatcggtggc agtatttcta tcgctctagt ttggcgggag ttgttttcta
    14881 aaaacgggtt agtgaatatt ttcctggaac gattaggcgc acctgcgctt tcctggttta
    14941 gcgatcaaaa actggtcatg atccctcttg ttttgatgtc tgcttggcaa ttcggttcgg
    15001 caatgatcat ctttgctgca ggtatcaaag agatcccagg ctcgtattat gaagcggcag
    15061 agatcgatgg agcgaacaaa tggcagacgt tttacaagat cacgttacct cttctttcgc
    15121 cgatcatttt gtataacttg gttatgcaga caatcgcggc gtttatgact tttacgcagg
    15181 catttatcat tacccaaggt ggtccgaatg atgccacgaa cttttatgct ctttatgtgt
    15241 ataaccaagc tttttctttt aattctatgg ggtatgctag tgctatttcg tggatcatgc
    15301 tgttgattat gggagctgtc acctttctga ttttcaaatc atcaaaaaaa tgggtcgttg
    15361 tgtgaatgag aggaggaaga aatgaataat acagtaacga aatggtttca atacggtttt
    15421 attttgctat taggtctagt catgatttat ccggtcttat ggatgatcgc aggttctttt
    15481 aaaacggatc aagagattat gagtggttcg ctgaatttga ttccagagac ttttcaatgg
    15541 gaaaattacg caaaaggatg ggcggggttt gctggaattt cattttttac tttctttagg
    15601 aattcgtttg tcatctctgc agtttcgacg atagggacag ttctgagctc gacttgtatt
    15661 gcttacgcat tatcgagaat cgatttcaaa ggaaaaagat tctggttcgt cgtcatgtta
    15721 gtaacgatga tgattcctgc acaagtgatt ttgattccgc agtttattat ttacaatcgg
    15781 ctgaatttgg ttgggacgta tgtgccgctg attttaccac atttttttgg acaagcattt
    15841 ttcatttacc agatcatgca attcatggtc agcatcccca aagagttaga tgaatcagcg
    15901 attattgacg gatgcagcaa gtatagtgtc tttaccaaaa ttatttttcc tttgctgaaa
    15961 ccttcgatca tcacgacgat catcatccag ttttattgga aatgggacga cttcatggga
    16021 ccattgattt atttaaacaa gccgagatct tacacggtat cgattgccat caaattattt
    16081 gcagacgctg gatctagtac gacttacgcg agtatgtttg cgatgtctac gttgtcgctg
    16141 ctaccggttt ttctgatttt cttattcttc aataaatatc ttgtccaggg gatcagtaca
    16201 agcggtctga agggctgaat atgaaaaagc caatagaaga gatgtttctt tacaagctca
    16261 actactatgt ctgtcggatt tttttaacca gtcttatcag taatgctctt ttcgctttag
    16321 ttctaggatg tttgttcact ttaggattag tgctgtctgc tcgtgttgtc tatctgattt
    16381 attttttgat gatgtatcct tcttattatc tatctgtcca ttacttggca gaaaaaaagg
    16441 tatgttggag gaaaggaaaa atcaaacgga tgtgtttcat cattacgttg tttcagctgt
    16501 ttgtcattat ttttgcagtt gattggtatt tattagtggg aaagattgga gcttggtatc
    16561 tgattccttt tctattgtgt ctgttgcttt ttgtatccca tagttttttt agtacgctaa
    16621 ttgatatctg caaagaagat aaagcagata gtcttgttta tttgatcaaa aggaatatct
    16681 ggctgttgtc aaaaaacgtc aaggggtttc tgtggtattt atgccttttg tatggactgt
    16741 tctggtgtat acagcatgat tttttactca ctgcattact gtatccaggg ctgccatttg
    16801 tcacagtatt ttgcttaaaa gaaaaggaga atgactttaa atgaagaaaa aatatgtaca
    16861 agtcggactt ggtgggagag cgcgtttttt ttatgaggca attgcctcta cgtatcatca
    16921 aacttgtgaa ctagtcggtt tttgtgatgt atctcaaaca aggatggatt atgcaaatcg
    16981 gatacttgtg aacaagtatg gacagaaaaa agtaaagaca tgtatgccat cacaatttga
    17041 gcaactactg gatgacgtac ggccggctga agtcattatt acgtcagtag atcgaacgca
    17101 tcatgattat atcattcgtg caatgaaaaa aggatatgat gttatttgcg aaaaacctct
    17161 gactatcgat gaggaaaaag ctcaagaaat aataacggtc caaaaacaga cgggaagaag
    17221 cctgcgagtc acttttaatt atcgttatgc accgtatcac acgaagataa aggaactgat
    17281 agaaagtgat gtgattggtg atgtgttttc tatccacttc gaatggctgt taaatacgca
    17341 acacggggca gattatttca gaagatggca tcgaaataag cacaatagcg gcgggcttct
    17401 tgttcataaa tctacccatc attttgatct agtgaatttt tggttgaaca gccgtcccca
    17461 aaaggtattt gcattcggtg gattaaactt ttatgggatg cataatgcgg agaaacgcgg
    17521 agtagaaata ttttacagtc gatgctatgg aagcgaacta gctaaagctg atccatttgc
    17581 tatagacctg tcagccaatg aggaattgcg agaaatgtat ttagaagcag aaaaagacag
    17641 tggatatatt cgtgaccgca gtgtattttc cgatgatatc tctattgaag atacactagg
    17701 cgtaacggtc aaatatgaaa ctggtgcgat tttgacgtat tcgctaaacg cgtatatgcc
    17761 gtgggaagga ttcaatgttt ctttcaatgg cagcaaagga cggatcgaaa tgaaagtgat
    17821 cgaaaagtct tacatcaatg caggtggaga taaaaaggat gaaggagcag ccgttcttca
    17881 gtccatccat gtctatccga tgttcggtga gccatatgag gtaatcgtcg aacaagaagc
    17941 gggcggacat ggcggaggag atccgcgttt attagatgat ctatttggtg aagaaaaaga
    18001 agaagatccg tggaatcggg cagcaactca cgtagatggt attttgtcga ttttgacagg
    18061 aatcgcagca aatcattcta ttgcttcagg aaaagcagta gtgattgacg agcttgtcag
    18121 cttaaaagaa taagaataga tttacggaaa ttacttcccg tagtttataa aatcttgcct
    18181 tattgccgat aggctgtctt ttgaacacta tttattcgga aaaaggggct atgacatgct
    18241 ttttgtcatg ccttcttttt atgtgggctg cttttttaga taggagaaaa taaaaataac
    18301 tatttaaacc cgaacaaaaa ggaataatgc attaaaaatc attagatttt acaaaaaaat
    18361 aagtaaacca cgttgtcagt ttcagaaagt agtgttagac tgttttcaat atttgaaaca
    18421 acatttttta ttcaaatatg gcaaactgcc ttcacgtgca aaacagttgc aagaaaatga
    18481 atttacacta ttattagttt tacataccta cttatatatc gtcataatat ggatgacagt
    18541 ttctagccag taccgtaaat gctggactat aagtaaaaga ttggctattt cacccttctt
    18601 ttcttatgat aaagtggagg gtgtttttct ttaacagaaa catagtaggt ttttgcacaa
    18661 aagcggttat ttcggtatta ataaatgata aaggagcaaa taaagtgaaa gaattagttg
    18721 aacgtattca aaaagacgga cgtatattag gcgagggcgt attaaaagtc gacagcttcg
    18781 tcactcacca agtagaccct gtattgatgg aacaaatggg aaaacggttt gcagaagtat
    18841 ttaaagaaca aggaatcacg aaagttgtga ccattgaagc ttctgggatc gcaccagctt
    18901 tatttgctgc aagagaatta gatgttccaa tgatcttcgc tagaaaagca aaaagtttga
    18961 cgatggatga agaattattg acagcttctg tttattcttt tacgaaacaa gtgacaagta
    19021 cgatttctat ttctcgtaaa tttttatctt cagaagacaa agtattgatt atcgacgatt
    19081 tcttggcaaa cggacaagcg gcaaaaggat tgatcgaatt atgccagcaa gctggagcgc
    19141 aagtagaagg aatcgggatc gtcatcgaaa agtctttcca agatggacgt cagttacttg
    19201 aagaaatggg attgagagtc gtttctcttg cgcgtattgc ttccttgacg aatggacaag
    19261 ttgagtttct tgaggaggac gcataatcgt ggaaaaagaa acacaaaatg gaaaagctgc
    19321 ggttttaggg ctgcagcatc tgctagcgat gtatgcagga gcagtagcgg tgcctctttt
    19381 gatcggtaca ggactaggct ttaatcaaga acagatgact tatttgattt caattgatat
    19441 ctttatgtgt ggattagcga cattattgca attgacagtt aaccgatttt tcggaattgg
    19501 cttgcctgtc gttttaggat gtgcgatcca agcagtagct ccactgatct tgatcggaac
    19561 caatgaagga gtaggagcta tctacggatc tattatcgct tctgggatct ttgttgtgtt
    19621 agtttctggt gtcttttcta aaattaaaaa actattccct cctatagtaa caggaacggt
    19681 tatcaccgtt attggtttga ctttgattcc tgtagcaatc gagaaaatgg gtggaggtaa
    19741 tgcaacagca gctgattttg gtgacaaaac aaatctgcta cttgcatttg tgacgattct
    19801 cctaattgta ggcgtgcaaa tgctggcaaa agggttcatt cgttcaatcg cggtattgat
    19861 tggtttagtc ggaggaagca tcttagctgc tttcatggga atgattgatc tgagcgcgat
    19921 tggagatgcg ccgttgttcc atgttccaca acccttttat tttggtaaac caactttcga
    19981 cgtatggtcg attttactaa tgatcatcat ttccatggtc agcatggtag aatcaacagg
    20041 tgtatatttt gcacttggtg acattactgg gaaaaatgtt ggagaagaag aattgaaaaa
    20101 gggatatcgt gcagaaggac ttgccgttat cttaggcggt attttcaata cattccctta
    20161 tactggcttt tctcaaaatg tcggattggt tcaactttcg ggaatcaaaa caagaaaacc
    20221 catctatttc tctgcattct tcttgattgt tttaggatta ttccctaaaa tcggtgcgtt
    20281 agcacaaatc atccctgagc cggtacttgg tggaggaatg ttagtcatgt tcgggatggt
    20341 ggcagtccaa ggaatccgta tgctgttgga agttgatttc agcaatgata aaaatctatt
    20401 gattgttgct gtttctgttg gatttgggct gggtttcaat gttacgccga cattattcag
    20461 tcaaatgccg caaacattgc aaatgttcac tggaaatggc atcgtaatga gtagcattac
    20521 ggctatcata ttgaatttga tctttaatgg attgaaaaaa gatgaagaaa tattggaaga
    20581 agccttcaag aagtaataga gcaaaagaaa ggttgtgaaa tcagcgtttt gacctgattg
    20641 ttagagaaac aaacggaaaa atagttcctc gtattttgcc gtttgtttga gctaataccg
    20701 agggtcagct gattgaacac cgttgaagga agaggtcgtg agcctgccgt tcatctccga
    20761 gtcacaagga acaatttcaa aaaaaagctt ctcatgtttg ttgaatattg ttccttgtga
    20821 ccgaggagtt ggcaggagaa caccgtttat ccataaaaag gaatgtgacc taacttttgt
    20881 cacattcctt ttttttatct ttaataaaca cgaacaataa aatgtttttc tttgttaaca
    20941 ttcgaaattt ctttgacatc aaaaaaagtg attgttaaaa tatgaatgta gaaaaactgg
    21001 gaggaaaaac aatgacagca aaaatctctg tgattatggg gagtacttca gattgggaaa
    21061 caatgaaaga agcatgtcaa atattggatg aattcggtgt gtcttatgaa aaaaaggtcg
    21121 tttctgctca tcgaacccca gatttgatgt ttgaatatgc acaaaacgct cgaaaaaaag
    21181 gaatcaaaat cattattgca ggagcagggg gagccgcgca tctgccgggt atggtcgcat
    21241 caaaaacaac tttgccggtt atcggtgtac cggtccaatc acgaacatta aatggtttgg
    21301 attcgcttct ttcgattgtc caaatgcctg gaggagtacc tgtagctact acagcaatcg
    21361 gaaaagcggg agcaattaat gctggactac ttgcgataca gatgctttcc atgtatgatt
    21421 tagaattgga ggctaaattg gctgaaagac gttcaatgtt ggctaaaact gttatagaaa
    21481 gtagtgatca acttggttaa acctttgttg cctggttcaa cgatcgggat cgtcggtggc
    21541 ggtcaattag gtcgtatgat ggcgattagt gcaaaagaaa tgggctttaa agtaggtgtg
    21601 ctggatcctg tgagcgattg tccagccgca caagttgcag attggcatat tgaaagtgtc
    21661 tatgatgaca cctttgcgct gaaagagctt gccagaagaa cagatgtcat tacctatgaa
    21721 ttcgaaaacg tcagtgtaga ggcgttaaat gcgattttac ccatgtcatt tattcctcaa
    21781 gggacagact tgcttgcgat tacgcaagac cgcctgttag aaaaatcttt tttggaaacg
    21841 aataacatcg tgattgctcc ttatgcaacg atcgtcagtc cgacggatat ccaagatgcg
    21901 attgacggga tcggctatcc ttgtgttttg aaaaccactc gaggcggcta tgatggcaag
    21961 gggcagtatg ttttgaaaag tcgggcggat ctagctcctg ccatggacct tttaagagaa
    22021 ggaacctgtg ttttagaagc atggatcccg tttgaaaaag agatttcgat catggtggca
    22081 ggaaatggtc aaggagattt cacgactttt ccggtcgtgg aaaacatcca ccacaacaat
    22141 attcttcatg aaacgattgc tccagccgcg attgatcagg atgtgatcga ggaggctgag
    22201 cgtattgccc gtgtgattgc agaagcagtg agcctttcag gtgttttagg ggtagaaatg
    22261 tttttaacaa aaacaggcgg gctatatgtg aatgaattag ctcctcgtcc tcacaattct
    22321 ggacattatt ctattgaagc ttgttctatg agtcagttcg atgcccatat tcgaggtatt
    22381 tgtggttggc cattaggaga aaaagtacgt ttgataagca atgcagtaat ggtcaatgtt
    22441 ttgggagaac aactgctgga aagctatcgc ttgattgaga ataagccaga ctggcaattc
    22501 catttctatg gaaaaaaaca agcaaaaaaa ggtcgcaaga tgggacatat cactatcttg
    22561 acagaagaca tccagcaaac gctggaagaa atcaaacaaa cgaatatttg ggattagagg
    22621 agaaaaaaca tgttagaacg ttatacacgc cctgaaatgg ggaatatctg gacggaagag
    22681 aacaaatacc gtgcgtggct cgaagtcgag attttagcag atgaagcttg ggcagaactt
    22741 ggtgaaatcc cgcaagaaga tgtgaaaaaa attcgtgagc atgcatcatt tgatatcgat
    22801 cggatcttgg aaatcgaaaa attaacacgc catgatgtcg ttgcgtttac acgagcggtt
    22861 tctgaaacat tgggagaaga aagaaaatgg gtccattatg gtctaacaag tacggatgtc
    22921 gtagatactg cttatggcta tctcatcaag caagcaaatg atattttacg ggaagacttg
    22981 aaacgtttta cggaaatcat tggcgaaaaa gcaaaagaac acaaatatac agtgatgatg
    23041 ggtcggacac acggtgtcca tgctgaacca actacttttg gattgaaact tgccacatgg
    23101 tattcagaaa tgaaacggaa catcgaacga tttgaacatg cggcaaaagg agtagaagca
    23161 ggaaaaatct caggagcagt gggaacgttc gcaaatatcc cgccatttat tgaagagtat
    23221 gtatgtaaac aattggggat ccgtccacaa gaaatttcta ctcaagttct ccctcgggat
    23281 ctacatgctg aatatttctc agcgatggcg cttattgcta caagtattga acgttttgca
    23341 acagagatac gcggattgca aaaatccgaa acacgtgaag tagaagaatt ttttgcgaaa
    23401 ggtcaaaaag gttcttctgc tatgccgcac aaaaggaatc cgatcggttc agaaaatatg
    23461 gctggtctag ccagagtggt gcgcggacac atggtcactg cctatgaaaa tgtcacatta
    23521 tggcatgaaa gagatatttc tcattcttca gcagaacgaa tcatcttgcc tgataccacg
    23581 attttattga attacatgtt aaatcgtttt ggcaatatcg ttaaaaatct aacggtcttt
    23641 cctgaaaaca tgaagcggaa tatgtctgca acatttggtt tgatttatag ccagcgcgtt
    23701 atgctgaaat tgatcgataa gggaatgaca agagaagaag cttatgatct tgttcagcca
    23761 aaaactgcgt acgcttggga caaccaagtg cagttccgcc cgctgttaga agaagatcca
    23821 accatcacat cggtattatc taaagaagaa atagacgatg cctttgatta tcatcatcat
    23881 ttgaaaaacg ttgacgtgat attcaaacga gtaggtttgg aataaagaag agtgagttgc
    23941 aaaattcaat taaaatagaa tagcaaaaat aaccaaaaaa gaagcagatc cgttagcggt
    24001 tctgcttctt ttagtttatt atattttctt atcaataata ggaacctgat tttaaaagcg
    24061 gctacctctt taccgtgcct ctttacatac aaaaaataga ggaattaagc gtaaatggct
    24121 tggctattat aactaaaatg ctggttatgg agatgttgca gcaagttttc gagaaaagcg
    24181 tattccatct cttgtccatt gaataagatc tggatacgat aatgattttt gaaacgcttc
    24241 tgttcatgaa tctgactgat tgaaagttcg cctttgtgtt ctgtctgata aagaaaagtc
    24301 cttttacggc attcagcaca gcgcttggtt gctcggcgga tttttcggaa tgtataaaaa
    24361 ctagtatctt tattgcataa ataaacgtct ttgatcgaca gcaaatcatt atatagacag
    24421 gaacgttctt cagctgttaa ttctgataaa aaaagctgac cttgttttat tgcatcttcc
    24481 atcggcaagt gcctcctcga acccattaac caaattttta gttaattcta atataccatc
    24541 attaagtata aatgtaaacg cttgttgatg gtataagatt ttcgtttctg ataaaacacg
    24601 aacattaaaa gaatctaatg ccttaaatgt acatattttt gttgatttat aaaaagatga
    24661 ttgctaaaat ttaaagtgaa aaaacgaaca aaaaacgatg tatttgcttt taatgtaaat
    24721 aaaaaggaga gacgtacatg gagaagaaag aacttttgta tgaaggaaaa gccaaaaaaa
    24781 tatatcttac ttctgatgag aacttgattt ggatagaata tttagatcaa gcaactgcgt
    24841 tgaatggcgc gaggaaagac cagataagtg gaaaaggaga attgaataat caaatcacaa
    24901 gtgcaatttt ccgttatctg actcaaagag gaataaaaaa tcattttatt gaacaattgt
    24961 ctaaaaccga acaattagca aaaaaagtac aaattatccc tctggaagtg gttatccgaa
    25021 atattgcagc aggaagtttc tcaaaacgtc taggtgttcc cgaaggtaaa gaattcatca
    25081 aaccaatcat tgaattctac tataaggatg accagttgga tgatccattt atcaatgaag
    25141 agcatatcta tttcttacaa cttgcaaagc cggaagaaat cagtgagctg aaagcccaag
    25201 cgttggctat taatgaaatt ttgaccgaat tatttgctaa ggtcggtttg gttttagtag
    25261 atttcaagct agaatttggc cggtcgaatg gacaaatttt gcttgcggat gaagtaacac
    25321 cggatacttg ccgattatgg gatgaacgta cgaatgaaca tatggacaaa gacgtttacc
    25381 gcagagatct aggtgatttg atcccagtct atcaagaagt atataaccgt ctatcggaac
    25441 taactgaaga aaaggagcga gtatgatgta tttcgtaaaa gtatatgtaa cctataaaga
    25501 ttcagtgtta gatcctcaag gggaggcagt aaaaaaagca gtccatcgta tgggatatga
    25561 aacaatcgaa gatatccgaa taggaaaata ctttgagata caacttgctg atgcagaaga
    25621 accaatcgat acagtgattg aaacgatttg tgacaagctg cttgcaaatg taaatatgga
    25681 aacataccga tatgaaatca aaaaaatgga ggaagtataa catgaaattt gcggtcatcg
    25741 tttttccagg ttcaaattgt gacgcagata tgttatgggc agttcgcgag atcatgggcg
    25801 ctgatgccga atttgtacgg catgatgagt cctctttaga aggatttgat ggggttttac
    25861 tgccgggagg attctcctat ggtgactatt tgagatgcgg tgcgattgct cggttttcta
    25921 caatcatgga agaagtgatt cgttttgctg aagaagggaa accagtattc ggtacatgca
    25981 atggattcca gatattgaca gaagcaggac ttttgccagg tgtactgaga cgaaatgagt
    26041 ctttgcattt tatttgcaaa actgtaccat tgaaagtcaa ccccaagacg gcctttactt
    26101 cagagtatga agccgacgaa ataatctatc ttccagttgc tcatggggaa ggaaattatt
    26161 tctgtgacga agaaacactt caagaactaa aaaaaaataa tcagattgcc tttacttata
    26221 atgacaacat caacggcagc gtggaaaaca ttgcagggat tacgaacaaa gcagggaatg
    26281 tgttaggaat gatgcctcat ccagaacgtg cagtagaaga cttgctagga tcagaagatg
    26341 ggaaacggtt ctttgcttcc attttaaaaa acttcggaaa ggtgaccgta tagatgaaaa
    26401 cattgaaaga accgacagct gaagagatca agaaaaaaag aatctatgcc gaatggggat
    26461 tgactgatga agaatatcga atgatcgaag aagatatctt aggaagaatg cctaattata
    26521 cggaaactgg actgttttct gttatgtgga gcgaacattg ttcctataaa aattctaaac
    26581 cggttctacg caaatttcct actagtggac ctcatgtgtt gcaaggccct ggagaaggcg
    26641 caggcatcgt cgacatcggt gatggactag cagttgtctt caaagcagaa agtcacaatc
    26701 atccttcagc cgtggagcct tatgaaggag ctgcgacagg agttggcgga atcatcagag
    26761 atattttcag catgggggct cgtccaattg cgttgctaga ttcattgagg ttcggcgaat
    26821 tagataatcc aagaacgaaa tatttactag aagaagtagt tgcaggtatc ggcggatacg
    26881 gcaattgtat cggcataccg actgtaggcg gcgaaattgc atttgatcct tgttatgaag
    26941 gaaacccatt agtcaatgca atgtgcgtag gtttgattga acacaaacat atccagaaag
    27001 ggcaagccgc tggcgtcggt aattcgatca tgtatgtagg tgcaaaaaca ggtcgcgacg
    27061 gtatccacgg agcaactttt gcctcagaag aatttaatca agaagaagaa cagcaacgtt
    27121 ctgctgtaca agtaggcgat ccttttatgg aaaagctgct gcttgaagcg tgtttggaat
    27181 taatcttgca tcattcggat tctctaatag gtatacagga tatgggagca gcaggacttg
    27241 tttcatctag tgcggaaatg gcttcaaaag ctggctcagg acttatattg acattagatg
    27301 aagtcccaca aagagaaact gggatgacac cttatgaaat gatgttatcg gaatcacaag
    27361 aacggatgtt gatttgtgtt aaatcaggag aggaagaaaa aatccaagaa ctatttcaga
    27421 agtatgatct agatgcagtc actatcggta aagtaacaga cgacgggcaa tatcggctat
    27481 atcatcaagg agaagaagta gcaaatctgc cagtcgatgc attagctgaa gatgcaccag
    27541 tttatcataa agaaatgaaa gaaccagctc gaatcgtaga atttcagcag ttagctcctt
    27601 atcagccagt tatcgaagaa cctggacaag tattgcttga tctgctgcag caaccgacga
    27661 ttgcttcaaa acgttcaatc tacgaaactt atgattcaca agtacaaacc aatacagttg
    27721 tccgtccagg aagcgatgct gctgtgatga gggttagagg gacgaataaa gcgttagcta
    27781 tgaccacaga ctgcaacgcg cgctatctgt atttggatcc aaaaatcgga ggacaaatag
    27841 cagtggcaga agctgctaga aatattattg ctagcggtgg taagcccttg gcgatcaccg
    27901 actgcctgaa ctatggttct cctgataaac cagaagtttt ttgggaatta tcgacttcag
    27961 ctgacggaat tgctgctgcg tgtgaaacgt taggaactcc tgttatttca ggaaatgtct
    28021 ctttatataa tgaaacggat ggtcaagcaa tctatccaac accaatgatt ggtatggtcg
    28081 gattgattga ggatcataaa catatcacta ctcaggaatt caaaaaatca ggggatctaa
    28141 tctatatatt aggtaagacg tttgcagatt ttgatggcag tgaattacaa aagatgcagc
    28201 ttggaagaat cgaaggtgtg atccgaaact ttgatctatc cattgaaaag agaaaccaag
    28261 agttagtatt gacagccatc caaaacggat tgattgaaag tgctcatgac tgttcagaag
    28321 gtggcttggc aattgcactg gcagaatcag ctttcaaaca tcagctaggg atatctgtac
    28381 agtttgaatt gtcctctgct caattatttg cagaaactca atcaagattt gtgttgacag
    28441 tcgcaccgga aaataagaca cgattcgaag aaatgatggg agatgctgct gttttagcag
    28501 gtaaggtgac ggatgaagcg ataatcgaaa tttcagcaac tgacggtcag atcaaaatag
    28561 aaactgcagt agcaagaaag tgctgggagg atgcaatcgt atgtcttatg aagtgaagag
    28621 tttaaatgaa gaatgcggga tcttcggtgt ttggggtcat ccagacgccg ctagtgtggc
    28681 atacttcggg ctccatagtc ttcagcatcg aggacaagaa ggtgcaggta tcgtgtccaa
    28741 tgatcgagga aagcttaagg ggcatcgaga tttggggctg ctttctgagg tattcaaaga
    28801 tcaaagaaaa ttgcagcagt taacaggaga agctgcaatc ggacatgtac gttatgcaac
    28861 agcaggcaat ggaagtgtgg acaatattca gccttttcta ttcaaatttc atgatcagtc
    28921 gatcggctta gcgcataatg gtaacttgat caatgctcgt tcccttagaa aagagttaga
    28981 ggaaaatggg gcgatcttcc attctaactc cgatacggag atattgatgc atttgatccg
    29041 acgaagtgaa gaaacagatt ttttaaataa attaaaaagt gctttgaatg aagtcaaagg
    29101 tggctttgcc tatttattaa tgacagaaac ggcaatgatt gccgctttgg acccaaatgg
    29161 ttttcgtcca ttagcaatcg gacaaatgaa aaacggagca tacgtgattg cctcggaaac
    29221 ttgtgcatta gaagttattg gggcacgttt tataagagat gtccagcctg gtgagatagt
    29281 gatcatcaat gacgaaggaa tccagattga ttgctttacg aaggacacgc agctttctat
    29341 ttgttcgatg gaatatatct attttgccag accagattca aacatcgcag ggatcaatgt
    29401 gcatactgca cggaaaaata tgggacggaa tttagcgaaa gaatcgccag taaaagcaga
    29461 tatggtcgtt ggcgtaccta attcttcttt gtcagccgca agcggttatg cggaagctag
    29521 tggaatccct tatgaaatcg gattagtaaa aaatcaatac attgcccgaa catttatcca
    29581 gccaacgcct gagctaagag agcaaggtgt tcgaatgaaa ttatctgctg tacgaggagt
    29641 agtggaaggt aaaaaggtga ttatggtcga tgattcaatt gttcggggta cgacaagcag
    29701 acggatcgtc catcttttga aagaagcagg ggcaaaagaa gtccatgtga ggattgcttc
    29761 tcctcctttg aaatatccat gtttttacgg gattgatatt caaacgcgta aagaactcat
    29821 tgcggcaaat cacacgattg atgaaatcaa agaatgtatc ggtgcagatt ctttaggttt
    29881 tttaagtgaa gagggtctga tcgaatcgat cggtctggaa aaagatgctc cgtattcagg
    29941 attgtgtatg gcttatttca acggagatta tccgacgccg ttatatgact atgaagaaaa
    30001 atatcaggaa tcactaaaag aaaaagtatc gttcttttaa aagcagacca agaagggagt
    30061 atatgaattc aatggcaaat gcatacgcaa aagctggcgt agatgtcgaa gctggatatg
    30121 aagtagtaga acggatcaag aagcatgtca aaaaaacgga acgtttaggt atgatgggcg
    30181 ctataggagg attcggcggt tgttttgatt tatcggtctt tcaattaaaa gagccagtcc
    30241 ttgtctcggg gaccgatggc gtggggacga aattagcagt ggcaatcgaa gcagacaaac
    30301 acgatacaat cgggatcgac tgcgtagcga tgtgtgtcaa tgatatctta gcacaaggcg
    30361 cagagccgtt atatttctta gactacttag caacggggaa aaatgatccg ttacgtttgg
    30421 aaaatgtagt aaaaggtgta gcagatggat gtgttcaagg aaatatggcg ctgattggcg
    30481 gagaaacagc tgaaatgcct ggtatgtact caggtaatga ttatgatcta gccggatttg
    30541 ctgtagggat agctgaaaaa tctcaattat tgacaggaga aacgatcaac ccaggagatg
    30601 tgttgcttgg aatagcatct agcgggattc attcaaatgg gtactctctt gttcgcaaaa
    30661 ttttctttga gcagcagcag ttttctttat catcaaaact gccagaattg aaaaaaacac
    30721 ttggcgaaga attgcttgag ccaacgaaaa tctatgtttc ttccttgctt ccgctgatca
    30781 aagaaaagaa aataaaagga gctgcacata tcactggtgg agggtttgta gaaaatatcc
    30841 cgcgaatgtt accagaatct atggctgcac aaatcaaact tggctcatgg ccgattcttc
    30901 caatattcgg ctgtttacaa aaatatggtg aaattcctga gatggagatg tatgaaatct
    30961 tcaatatggg catcggtatg gtcctggcag tcgaagaatc gaaagtaaaa gaaatagtac
    31021 acgaaattga gcaagctggc gaaaaagttt atgaaatcgg gaaagtgatc gccaaagaaa
    31081 aacatcgtgt ggtgtttata ggagaagaac gatgagaatc gctgtttttg cttctggaaa
    31141 cggaagcaac tttcaagcgc tagccgatta tctatcgaaa aaaggattgg aatcttcgat
    31201 tgactggttg ttttgtgatc agcctgaagc atatgttttg aaacgtgcaa ctgcgttgag
    31261 tgtaccggca gattgttttt cacctaaaga atttgattca aaaaaagagt atgaagaagc
    31321 aattttgcat aaattgaaag aaaaaaagat cgatttgatc gtattagctg gatatatgcg
    31381 aatcattgga ccggtattgc tgaaaaatta tgataaaaga atcatcaata tccatccgtc
    31441 tcttttacct gccttccctg gtttgcatgg catacgtgat gcctttgaag ctggagtaaa
    31501 agaaacgggt gtcacaattc attatatcga ccaaggtgta gatactggac caatcatccg
    31561 gcaggaaaaa gtgagaattg aacaggagga cacgttcgat tctttagaag aaaaaatcca
    31621 tagagtagag catcgaatct atccagaagt gattagtgaa attattgaaa atggaggcta
    31681 tctgaaatga caagagcatt gatcagtgta tcagataaaa ctgggattgt agcattcgcc
    31741 caaggtttga ttgaacaagg agtagaaatc atttcaacag gaggaacaaa gcatgcgttg
    31801 gataaagctg ggatcaaaac gctttcgatc gaagaagtga cagggtttcc tgaaatgatg
    31861 gatggccgtg tcaaaacact tcatcctaaa atccatggtg gtcttttagg aagacgcgat
    31921 ctccctagcc atatggaggc aatggaaacg gaaaatatcc aaccaatcga ctttgtttgt
    31981 gtcaatttat atcctttcaa agaaacgatt atgaaaccag atgtcgaaat agcagaagct
    32041 attgaaaaca ttgatatcgg tggtcctagt atgctccgta gtgcagcaaa aaaccatgaa
    32101 tttgtcacct ctgttgttga tccatctgac tatgaagaag tactttcaga attacgtcag
    32161 tatgggcata cttcactttc tttcagacaa aaactagcgg caaaagtctt tcgtcatact
    32221 gcggcatatg atgcactgat cgctcaatac ttaacggatt gggtggaaga aaaagaaccg
    32281 gaaaaattga cacttacgta tgaacgaaaa caaacgcttc gttacggtga aaatagccat
    32341 caagccgctg ccttttatca gtccgctctt ctggaaaaat attcgatcgc agcagcaaaa
    32401 caactccatg ggaaagaact ttcttataat aatatccgag atgcagatgc tgcgctacgg
    32461 atcatgaagg aatatgacga gccaacggta gtcgccttga aacacatgaa tccttgtggt
    32521 attgggacag catctacgat tttagaagcg tgggaagcag cgtatgaagc agatcctgtt
    32581 tcaatctttg gtgggatcat cgttttgaat cgagaagtag atctgccaac agcagaacag
    32641 atgcataagc tgtttctaga aatcatcatt gccccttctt atacacaaga agctttggaa
    32701 ttactgcaaa aaaagaaaaa catccgatta ctgacagtag actttgcgca ggaagaaaat
    32761 caaaatcgag aagaaacggt ctcagtctta ggtggacttc ttgtacaaga tcaagatctg
    32821 gcaatagaaa atgaagaaga gtggaaagtc gtgacaaaac gtaagccgac tccagcagaa
    32881 gcaaaagcat tagtgtttgc ttggagagct gtcaaacatg tgaaatctaa tgcgatcgta
    32941 ctggcaaacg aacgtcagac agtaggaatc ggagccgggc agatgaatcg agtcggttca
    33001 gtaaaaattg cgattgaaca agcgcaagag aaaatggaag gtgcagtttt agctagcgat
    33061 gcttattttc caatggatga ttcggttgaa tatgcagcca aacatggtat aaaagcaatc
    33121 attcagccag gaggaagcat caaagaccaa gattctattg atatggcgaa taaatacgat
    33181 ctagcaatgg tctttactga tgttcggcat tttagacatt agagaggagt cggataaatg
    33241 aacattttgg tgatcggtgc aggtggtcgt gaacatgcaa taagtaaaaa acttttagcc
    33301 agtccgaagg tggatacggt gtattgtgcg ccaggaaatc cggggatgac aaaagatggt
    33361 atcaattgtg tcgctatcag tgagcgagat cattcagcgt taatccattt tgttaagaaa
    33421 gaggctattg attggagtat cgttggtcct gaagttcctt tgttaaatgg gatcgtcgat
    33481 gattttatgg cggaaggatt gaaaatcttc ggtccgacaa aagcagcagc tctgatcgaa
    33541 ggttcgaaaa gttttgctaa acaattaatg gatacttatc aaatcccgac agctaaatcc
    33601 agaagttttg aatgttacga aaaagcagca gcgtatatca aagaaaatgg tgcgccaatc
    33661 gtagtgaaag cggacggctt agcagctggg aaaggtgtag tcgtggctac cacagtgaag
    33721 gaagcgctgg atgcggcaga acaaatgttg aaccagcatc gatttgggga tagtagcaaa
    33781 aaaatcgtcg tagaagagtt tttggcagga gaagaattct cccttttggc gtttgtccgt
    33841 aataccaagg tgtatccgat ggtcatttcg caagatcata agcgggcatt tgataacgat
    33901 aaagggccaa atacaggcgg aatgggtgct tattcaccag taccgcaaat ttcaaaaaag
    33961 atgattgaaa aagctgtgga agaagtttta aaaccagcgg ctttgggaat gaaagaacta
    34021 ggacgctctt tcacgggtgt tttatatgcg ggtttgattg ctacgaagga aggaccaaaa
    34081 gtgatcgaat tcaatgcgcg tttcggtgat cctgaaacgc aagtcgttct ttctcggttg
    34141 aagagtgact ttgcgcaagt aatcgatgat ttattagaga atcgtacagt agagttaaag
    34201 tggcagcaag aaggatacaa tgtaggcgtt gtggttgcag ctgccggtta ccccgaagaa
    34261 tatgagacag gaatggttct accagatttt ctggaagaag agctttctgt ttactatgct
    34321 ggagtttcct caaataacgg caaactagtt ggagcaggag gacgtcttta tttagtagaa
    34381 gcatacggtg agacgatcaa agaagcacag aaaaaaattt acaccgcgtt aatggataaa
    34441 acgacagatg gaacatttta tcgtacagat attggagaaa aagctgccaa tagcgaactg
    34501 atcgtgaagt aaatcagctg cttccattta taaaaaatac aacaaacata atcggaagcg
    34561 ccgattttag tgaagccttt tcataaagag tgtggtacac ttttgttgtg tatataaata
    34621 aggaggagtt cagatgaaaa aaattcaatt aggtacttct gatcagaaag cagctgcagt
    34681 gatcttaggc tgtatgcgta tcaacaatgc ggaagatcca gtaaaagtga ttgagacagc
    34741 ttatgaaaac ggcattgatt tttttgacca tgcagatatt tatggaaacg gtgaatgtga
    34801 agaaatcttt gcagatgctc tagcaaaaac atcaatcaaa cgagaagatc tttttattca
    34861 aacaaaatgt ggaatcgttc caggtgtgat gtttgatttt tctaaaaaac atatcattga
    34921 atctgtagaa aaaagtttga cccgtctgaa aatggattat gtggatgctt tacttcttca
    34981 tcgtccagat acattggtag aaccagagga agtagctgaa gcgtttgatg aattggaacg
    35041 gtcaggaaaa gttcgttatt ttggcgtcag caatcaaaaa ccaatgcaaa tcgaattgct
    35101 gaaaaaatat gttcgtcaac cattagtagc taatcagctg caatttggct tgaaacatac
    35161 tggaatgatc gatcaaggga ttcatgtcaa tatgacagat gaagcaagta tcgatcatga
    35221 cggtagtgta cttgattatt cccgattgca tgatatgacg atccaagcat ggtcaccata
    35281 ccagtatgga ttttttgaag gtgtatttat cgggaacgaa aaattccctg aattaaacca
    35341 aacattagaa gaactagcag ctaaatatca aacgacacca actggcttgg cttctgcttg
    35401 gattttacgc catccagcca atatgcaagt gatcgccggt acgatgaacg tagggcgaat
    35461 caaggaaatc gctcaagctt cagaaattaa tatgtctcgg gaagattggt acgaattgta
    35521 ccgtgctgct ggaaacattt taccttaaag attgtcataa gcaggtcaaa agggctataa
    35581 attaaaaaga cagaataact tgaaagctgt tttcagcttc gagatattct gtcttttttt
    35641 attgcaaatg gaaagagtat cattgtacgg atttcgttta ttagttattt ttgtttttaa
    35701 ggaaaagctg tgtccgatat tcttttggag aaatcccttg ttttttctta aataaataac
    35761 tgaaatagtt agggtcatta taaccaattt caaaagcaat atctgaaata cgatagtctg
    35821 attgctgcaa taattttttt gctaattcga ttctttgttc tgtcagataa tcgatgaatg
    35881 tttggttaaa cgattgagaa aaaatcgtac tgaaatgaga gggacttaat gcaacttgtt
    35941 gagcgaccca agttaaggac atatctggat cggtaaaatt ttctttgata aaatccaatg
    36001 catggttcaa aattggctga tatttgttcc ttgaaggctg tgagggcaga gcagttaaga
    36061 aagaaatgaa catataaact gtcgaacggt aaagcgagtc gtcggcgctg attgctaagc
    36121 gttgactggt ggatgtgcaa tgaaccattt cttgcgggat tttcttttct ttcaggcaga
    36181 catgctggag tagcttagtc aagacaaata aacgatataa tcgtgttcga gttggtgtgc
    36241 cttgtggttt cgtcaagtgt tcgatgtact tagatacttc gtttttcgat aatgaagaca
    36301 aatcaagtga gaggtcaaag taaaagacag aatcaactgg aagtttcgct tcttgaatca
    36361 aatcctcata gctgataatt tgttctttaa gggaactggc ggtttcaagt atcatctgcg
    36421 tgatctcaaa agatgcaggt aattcgctga ttctttctgc gacaggacca atggcgacag
    36481 aaaaattatt tttttgtccc ttttctaatt catggaccaa cgtttgggct acctgataac
    36541 tggtctcgat cacattttct tcaagagaat caactattaa aaatttgatg aatcgagagg
    36601 aaacagcaga gaagatcact tgttgattct ccctaaataa aaacttcaaa taatcactga
    36661 gatgagcgta atcttcaaag tctgtactga cttggtttgc tgctaataat acggtcattt
    36721 ttttccctgc taaagagcga ttgaaagatt cagcaagttt caatgtttca ctgatcgtaa
    36781 gtgagccttt gaacaaaccg ttcaagaaat gatttttttt caattcgaaa acaagatcag
    36841 aagaagtgtg cgtttctttt tgctgcaaat ctaaagcagt tactgctttt tctattgctt
    36901 gtttcaattc gctactttta atcggtttaa gcagataatc atctgcctgt acctggattg
    36961 ctcctttcat ataatcgaaa tcgtcgaatc cactaataaa tatgagacga gtccaaggaa
    37021 gtagtttcct tgcttctttt gaaaaagtca acccgtccat gaaaggcatt tggatatcag
    37081 tcagaatgat gtcgggctgt aagtcgataa ttgaagacag tgccatctca ccatctccag
    37141 cttcgccgac ataagtgatt ggataataag aaccaaggac agttatttgt tcccgcaagt
    37201 tattgcgaat gaggtattcg tcttcgatga taaaaacttt atacatgttt gtttcctcca
    37261 attgttttgg gagatagagg gatcatcagt gtgacagttg ttccttctcg gtatttgctg
    37321 tcaatcgtga taggtttttg atcatttccg taataaagaa ggagacgctt gtttacgttg
    37381 taaagtccgt agccgatact gaaatctgaa tcgatccctt tggctaactc tttacgtatt
    37441 tctgccagtt tttctgcagg gatgccaatc ccgttgtcta taactttgaa ttggatcatc
    37501 tcatcaattt tgcatgtggt aatacgaata aatcctttgt gacggacgta cttcgttcca
    37561 tggtatacag cattttcaat caatggctgc aagatcattt ttaaaataga aacaggaaaa
    37621 agtgtttctg gtatatcgat cttatagtca agcatatggc cgtatcttat ttttaagatg
    37681 actagataat cattgatatg gctaatttct ttttcaagag gaatccagtc ttgtcctttg
    37741 cttaaagacg tacgaaaaaa gccggaaagt gcatgggtca tagtcagagc ttcttcaaag
    37801 cagtcttgtt cgatcaaaga aataatggca tccaaactgt tataaataaa atggggagta
    37861 atctgagctt gcaatacacg aacttcactt tgtgctaaat ggtattgttt caaagcgttc
    37921 tcccgtaaag actggtttag atcttccgcc attttattca tgctagcggt aagtgtttgt
    37981 aattccagtg tttccggcag ggttaaacga aagccaaggt tccctgaaga aagttgttct
    38041 gacatctcga tcagctgatt gatcggttct tggacctttc tctctaaaaa gcgacgattc
    38101 tttttcgcat agaaaaaaat ggtactaaga actccaagtt ccactattgt taatagaatg
    38161 agcgagcgga caagtgtcgt attttttttg cttgctaagt cgatctccac acgaacaaaa
    38221 tcttgtaaga tatctgacag cagttgagtg acagaatcta cctgattgat gatctcttca
    38281 ttggtatcca taaaatcttt tgccaagata ttattgatca gctcgttttg ataatcttcc
    38341 agtgtatcga tcgtacgtag cgatacatct aacgtactaa tttctttaag tgtatttgta
    38401 ttatttttgc tctctcgaat atcattccta agttcttcta ggatattttc ctcattgaac
    38461 tcatctacgg ttacttggcc agaaatcaag tcccacatac tttccaaaac tttttgatca
    38521 accttcaaag caattgcatc tgctttttgg atatttgcga tcgtctgttg ataaatcaaa
    38581 agctgttgcg aatataacag tatgctcagt agcaaaggga taacagcaaa tagaagcatg
    38641 gctttgtttg cttgggcaaa aatctgtttt atgcttaaat tttttgtcat gatacttctc
    38701 cagtcaataa tttctggttg ggatgttagc tatattctgc tgggaaaaga cagtctcgga
    38761 aataaaaata tctttaggta atgtttttcc tgtttgaata tttccaaaat aacgagtaac
    38821 ggtattggct acataccagc cgacatcagg tttacactcg actacacaat tgacgattcc
    38881 tgagcgtagc ttctcgatca tatcttcttg tccatcaata gttacaatga tcaaatcttc
    38941 tccaggtttg attgatgttt cttttatcgc agctaaagca ccgtgagtca tttcatcact
    39001 atggctgtat aatacatcga tttcggataa atcatttgtt tctaaatatc gtttcatctg
    39061 gtcttttcct ttcatccgaa taaaatcacc gacaattcgg tgaacaatag tcatcggagg
    39121 tttatggtcg atggttccat ctttttttag ggttggttta cgattgattg attccaaaaa
    39181 tccttcggag cgtagcttgg tcggtgtgga gttctctgaa ccagcaagtt ctaggattct
    39241 gatttcctgt ttctttgaat ttgtgaagtg attgctgaca aataaacccg cccgatttcc
    39301 ttctgctttg aaactggggc caatgtgggt gacgaaaagc tcgggatcat tcgtttgaat
    39361 atgtctgtcg acaagtatga cagggatccc tgctcgcttt gcttctttta atacagagtc
    39421 ccacccggat tcttgcaaag gagtaaaaat gatcatatcc actttatagg caatgaacgt
    39481 ccgtatatct tggatttgcc gttcctgatt catgtagcca tttcggtaga gaacttggta
    39541 atcttctttt tcaagcgcag tgataattga ttcagtatgc tttttccgcc agctactctc
    39601 cgttcctgat tgcgaaaaac caacgactag tgggtcaggt gcttcctttg tattaggtgt
    39661 acaccccatc atttggtaag cgattacaac aagggctaaa aaaaataaaa cacgtttcac
    39721 tttaaatcac tccatctgga tttgttagta ctatcttatc agataaaagg gaaaatcaaa
    39781 gtcaaaagga atcaatcgaa aaaaatccaa gaaacttaaa aaaatccaag acaaaaaaag
    39841 tacacctaaa aaatttaaag taaatcccat aaaaaaatca atgttaacgc tttctttatt
    39901 agctatactc ggtgtatcaa ttgaaatggg ggataaaaaa tgaaaaacaa atttagaaag
    39961 attgttggga ctatcgctat tttgaccgca agcattctat taagtgcgtg tggcaattca
    40021 ggatctgcag atgatagtac ggggtatgta ggaatcgcta tgccgaccaa atcagcagaa
    40081 agatggatcg ctgatgggaa caacatggtc tcggaattag aaaaactggg ttataaaaca
    40141 gatcttcaat atggagaaga taaagtagaa aatcaagtag cacaaataga aaatatgatt
    40201 acaaaagggg tagatacact tgtgattgct tctatcgatg gttcggcttt aactgatgtg
    40261 ttagctaaag caaaagaagc agatatcaag gtgatcgcct atgatcgttt actgatgaat
    40321 tcagaaaatg tcgattatta cgcgactttt gataattttg gagtaggtgt atcgcaagca
    40381 gcttatattg aagagcactt aggattgaaa gaaggaaaag gaccattcac gattgaatta
    40441 tttggtggct cgcctgacga caacaacgca ctaatcaatt acaacggagt gatgtctgtt
    40501 ttacaaccat atatggacaa cggtcagttg gtcgtgccat ctggacagac gagtttcagt
    40561 caaatcgcta cgcttcgctg ggatggttct acggcgcaag caaggatgga taatctgtta
    40621 agcgcaaatt atactgacca aacattggat gctgttttgt cgccatatga tccaataagt
    40681 ttaggaatca tttcgtcatt aaaaggcgtg gggtatggga gtgaaagtaa acctttacct
    40741 gtgattactg gacaagatgc gacagttgct ggcgttaaat cgatcattgc cggggaacaa
    40801 acacaaacga tctttaaaga cacacgtatc ttagcaaaaa atacgattga aatgatcaaa
    40861 gcaatcagtg atggagaaga ggttccggtc aatgatactg aaacttatga taatggggtg
    40921 aaaactgtcc ctacgtattt agcaaataca gtatctgtcg ataaagataa ttatcaagca
    40981 gagctgatcg acaccgatta ttataaagaa tccgatctga aaaattaaga ggaaccaaac
    41041 aaggaactaa agagatctaa gtcagagaga ggataagaat tatggcggat tgtattttaa
    41101 aaatggcaga tatcaccaaa aaattttctg gaatccgagc gttagattgc gtgaatctga
    41161 tgattgaacg tggggaaatc catgcattgt gcggagaaaa cggagcggga aaatcaacat
    41221 tgatgaatgt cttgagcggc ttgtatccat atggctccta tgacggggaa atcttttatg
    41281 atggaaagct gtgtcaattt aaagatttga aagatagtga agaaaaaggc atcgtcatca
    41341 ttcatcaaga attagcacta agtccttatc tttcaataaa agagaatatt tttctcggaa
    41401 atgaacaagc agaaagaggc gtgattgatt gggaactgac agaaaagaaa acatatgaac
    41461 tgctgaataa agtcggtttg tctatcgacc cgaatcttct agtatcccag attggcgtag
    41521 ggcaacagca actagttgaa atagctaaag cattatcaaa atccgtccgt ctgttgattt
    41581 tggatgagcc taccgctgca ttgaatgaag aagaaagcgc gaatttgctg agattgatcc
    41641 gagagttgaa agagcaaggt gtaacctcca tcattatttc acataagctg aatgaaatcg
    41701 tggatatcgc agatcgaatc accattttac gggatggaca gacgattgag acactagaaa
    41761 atgaaatgat cagcgaagag catattattc gcggaatggt gggcagagat ttagttgatc
    41821 gttatcctga aagaaaaccg gatattggag aagtttattt taaagtagaa gactggacgg
    41881 tctatcaccc tcttgatcac gagcgaatca taaacgacca catcaacttg catttgaaaa
    41941 aaggagagat tgtaggaata gctggactga tgggagcggg cagaactgaa ttcgctatga
    42001 gtctatttgg acattcttat ggaagcaaga tatccggacg tatattaaag gaaggggcag
    42061 agatatcagt taagaccgtt ccttcagcca ttgcaaacgg actcgcttat gtctcggagg
    42121 accgaaaatc tttaggactg aatctgctga tggatattcg ggaaaacgtg acgctgagca
    42181 gtttggataa aataagtcaa aaaggagtat tagataaaga aaaagaagta actgaggcag
    42241 aaaatttccg gaaaaagatg cgtataaaaa caaactccat ccatcaaatt gtcagtagtc
    42301 ttagtggtgg gaatcaacaa aaagtcgttt tatccaagtg gctgatgaca caacccgata
    42361 ttcttttttt ggatgagcca acccgtggga tagatgttgg ggcaaaatat gagatctaca
    42421 cgattatcga agaaatggca gcaaatggca aagctgtctg tcttatctct tctgaacttc
    42481 ctgaaatatt agggatgtgt gatcgaatct atacgatgaa cgaaggacgt attacaggag
    42541 aagtctcgcg agaagatgca gatcaggaag tcttgatgaa attaatgacg aaagaagagg
    42601 tgggttaagt tggaacaggc aaaaaatctt acggataaaa aagagaaaaa actaaatttg
    42661 aaaagtagag cagctgatct ttttggtcgc tacagcatgg tgatcatttt agcgggacta
    42721 cttattgcgt ttcaattgat gacggatggt attttctggc gtccattgaa tataactaac
    42781 cttgttctgc aaaatagcca tatattagta ctagctgcag ggatgttatt agttgtgctc
    42841 ttaggtcatg tggatttgtc ggtagggtca gtgatggcgt ttgttggtgc aattgcgggc
    42901 atgatgatgg tcaatcgcca aatgaacccg tggatgacag ttatcgcatg tcttgcagta
    42961 ggcgggctga tcggcgcatg gcaaggtttt tgggtagctt atgcagggat accggcgttt
    43021 atcgtgactt tagcaggatt gctgatgttt cgtggactga cacaagttgt tctaggaggc
    43081 caatctctag caccttttcc agctgttttc caaaaaatgt ctacgggtta tttaccagac
    43141 ttaagcgaag gaagcagcct tcacttcttg acattgattt taggaatgat catcacgatc
    43201 gtgttagttg ttggtcaatg gcgaacaaga aatcgtcgga aaaatcattt atttgaagtg
    43261 gaatctccaa cagctttctt ggtaaaggcg gtatttacag cagtcgttgt actaggaata
    43321 acttatatct ttgcctctta tcaagggtat ccgattattt tgatcattct tggtttgatc
    43381 gttgcagcat atgggttttt gacaaataaa acagtagcag gtcgacagat ttatgcgacg
    43441 ggcggaaatc gaaaagcggc tgaactatca gggatcaaaa caaagcgaat cacgttctgg
    43501 gtattcgtca acatgggagt catggcagct ttagctggat tagttttagc tgctcgattg
    43561 aatgcggcaa cacctcaagc aggtacatcg atggaactgg atgcaatggc ggcagtatat
    43621 tttggtggtg cttcgacttc cggcggcatt ggaacaatca tgggtgcgat tgtcggtggc
    43681 ttggtcatgg gtgtattgaa caatggcatg tcgattttag gcgtcggggt agactggcag
    43741 caggcaatca aaggattgat cttgctttta gccgttgtgc tagacttgta taataagaaa
    43801 aaacgaacaa cgtaggagaa cgccggttat tcagaaaaaa gactgtgaca agacatttgt
    43861 cacagtcttt ttttcgctaa aagcgaacat tcattgtttc gtttgtatag actaaaaata
    43921 atatatgttt ttttaccatt cgtataaatt ctctttttgc tcttgatttt tttgcttaat
    43981 tgagttaaat tacatagggc gctaaaaaaa agccttgatt aaattaataa tgttcgtgtt
    44041 ttgctggatg tgaacatcag gaggataatg taatgaaagt atttgattac gaagatattc
    44101 aattaattcc taataaatgt atcgtaaata gccgttctga atgtgataca acggtcacat
    44161 taggtaaaca tacattcaaa atgccagtag tgcctgccaa catgcagacg attattgatg
    44221 aaacaatcgc agaattctta gcggaaaatg gttatttcta tatcatgcat cggtttgatg
    44281 aagcagctcg aattccgttt atcaaaaaaa tgaaaaagcg tggattgatt acctcgatca
    44341 gtgttggcgt caagaaagag gaatattcct ttatagagaa gttggcagaa gaatcgctaa
    44401 atcctgatta tatcactatc gacatcgcgc atggacatgc taactcagtt attgatatga
    44461 ttcagcatat taaaaaatat ttgcctgaaa cttttgtgat tgccggtaat gttggtacac
    44521 cggaagccgt ccgtgaattg gaaaatgcgg gcgcagatgc aacaaaagtc gggattggtc
    44581 caggaaaagt atgtattaca aaaatcaaga caggctttgg gactggaggc tggcaactgg
    44641 cagctcttag atggtgtgcg aaagcagctc gcaagccgat catcgctgac ggtgggatca
    44701 gaacacatgg cgatattgca aaatctgtcc gttttggtgc gacaatggtc atgattggtt
    44761 cattgtttgc cggtcacgag gaatctcctg gcgaaacaaa agtcgaaaat ggaatcgtgt
    44821 ataaagaata ctttggttct gcttcagaat tccaaaaagg cgaaaaacga aatgttgaag
    44881 gaaagaaaat ctggatccaa cacaaaggat ctttgaaaga tacactagtg gaaatgcaac
    44941 aagaccttca atcatctatt tcttatgctg gaggaagaga tctggaagcc attcgaaaag
    45001 ttgactatgt catcgtgaaa aattcgattt ttaatggaga cacaatctaa aaaa
//
LOCUS       DAZUMK010000011        43034 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000011 DAZUMK010000000
VERSION     DAZUMK010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.011"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            113..1339
                     /locus_tag="ACG808_000604"
     CDS             113..1339
                     /locus_tag="ACG808_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5046257.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGITSEKYQKVFPTRQLAIKA
                     ENAMKRKIETVLREENANSLELKGKIKFKEFYESKWLPRYELGQTTRSKRAPSYVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQCITAPKKIALEERRIREGNQALSSEELIAWIEAVNTDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFEKQTVRLKHALDKYQKKKFTKGRKDTV
                     IQVPEIVMALLSKWKSVQAEQLLRLKINQTPDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPELAHLSPHKLRHTYATIARQGGANMNQISSALTHSDISTTRIYVNTPDVV
                     DKTVFEAFQRGLNKCD"
     gene            complement(1428..1820)
                     /gene="rpsI"
                     /locus_tag="ACG808_000605"
     CDS             complement(1428..1820)
                     /gene="rpsI"
                     /locus_tag="ACG808_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFS5046258.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(1836..2264)
                     /gene="rplM"
                     /locus_tag="ACG808_000606"
     CDS             complement(1836..2264)
                     /gene="rplM"
                     /locus_tag="ACG808_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFS5046259.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(2798..3265)
                     /locus_tag="ACG808_000607"
     CDS             complement(2798..3265)
                     /locus_tag="ACG808_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFS5046260.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(3385..4923)
                     /locus_tag="ACG808_000608"
     CDS             complement(3385..4923)
                     /locus_tag="ACG808_000608"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFS5046261.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            5003..5689
                     /locus_tag="ACG808_000609"
     CDS             5003..5689
                     /locus_tag="ACG808_000609"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFS5046262.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(5890..6417)
                     /gene="msrA"
                     /locus_tag="ACG808_000610"
     CDS             complement(5890..6417)
                     /gene="msrA"
                     /locus_tag="ACG808_000610"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS5046263.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(6501..7178)
                     /gene="pgmB"
                     /locus_tag="ACG808_000611"
     CDS             complement(6501..7178)
                     /gene="pgmB"
                     /locus_tag="ACG808_000611"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFS5046264.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(7175..8305)
                     /locus_tag="ACG808_000612"
     CDS             complement(7175..8305)
                     /locus_tag="ACG808_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFS5046265.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(8305..10488)
                     /locus_tag="ACG808_000613"
     CDS             complement(8305..10488)
                     /locus_tag="ACG808_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFS5046266.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(10485..11510)
                     /locus_tag="ACG808_000614"
     CDS             complement(10485..11510)
                     /locus_tag="ACG808_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HFS5046267.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANAIRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(11555..12382)
                     /locus_tag="ACG808_000615"
     CDS             complement(11555..12382)
                     /locus_tag="ACG808_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFS5046268.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(12379..13446)
                     /locus_tag="ACG808_000616"
     CDS             complement(12379..13446)
                     /locus_tag="ACG808_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFS5046269.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(13472..14308)
                     /locus_tag="ACG808_000617"
     CDS             complement(13472..14308)
                     /locus_tag="ACG808_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5046270.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(14305..15174)
                     /locus_tag="ACG808_000618"
     CDS             complement(14305..15174)
                     /locus_tag="ACG808_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5046271.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(15244..16527)
                     /locus_tag="ACG808_000619"
     CDS             complement(15244..16527)
                     /locus_tag="ACG808_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5046272.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(16541..18226)
                     /locus_tag="ACG808_000620"
     CDS             complement(16541..18226)
                     /locus_tag="ACG808_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFS5046273.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            18501..19526
                     /locus_tag="ACG808_000621"
     CDS             18501..19526
                     /locus_tag="ACG808_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5046274.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(20121..23774)
                     /gene="rpoC"
                     /locus_tag="ACG808_000622"
     CDS             complement(20121..23774)
                     /gene="rpoC"
                     /locus_tag="ACG808_000622"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFS5046275.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(23853..27470)
                     /gene="rpoB"
                     /locus_tag="ACG808_000623"
     CDS             complement(23853..27470)
                     /gene="rpoB"
                     /locus_tag="ACG808_000623"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFS5046276.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            28198..29166
                     /locus_tag="ACG808_000624"
     CDS             28198..29166
                     /locus_tag="ACG808_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5046277.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            29184..30089
                     /locus_tag="ACG808_000625"
     CDS             29184..30089
                     /locus_tag="ACG808_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5046278.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            30086..30892
                     /locus_tag="ACG808_000626"
     CDS             30086..30892
                     /locus_tag="ACG808_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5046279.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            31049..32017
                     /locus_tag="ACG808_000627"
     CDS             31049..32017
                     /locus_tag="ACG808_000627"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFS5046280.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(32334..32858)
                     /locus_tag="ACG808_000628"
     CDS             complement(32334..32858)
                     /locus_tag="ACG808_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFS5046281.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            33145..34956
                     /gene="scm"
                     /locus_tag="ACG808_000629"
     CDS             33145..34956
                     /gene="scm"
                     /locus_tag="ACG808_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFS5046282.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSS
                     TTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKR
                     ALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(35111..36097)
                     /locus_tag="ACG808_000630"
     CDS             complement(35111..36097)
                     /locus_tag="ACG808_000630"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFS5046283.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(36120..36686)
                     /locus_tag="ACG808_000631"
     CDS             complement(36120..36686)
                     /locus_tag="ACG808_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFS5046284.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(36700..37116)
                     /locus_tag="ACG808_000632"
     CDS             complement(36700..37116)
                     /locus_tag="ACG808_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFS5046285.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(37573..39507)
                     /locus_tag="ACG808_000633"
     CDS             complement(37573..39507)
                     /locus_tag="ACG808_000633"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS5046286.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            39809..40744
                     /gene="pplA"
                     /locus_tag="ACG808_000634"
     CDS             39809..40744
                     /gene="pplA"
                     /locus_tag="ACG808_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFS5046287.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            40834..41937
                     /locus_tag="ACG808_000635"
     CDS             40834..41937
                     /locus_tag="ACG808_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFS5046288.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            41950..42903
                     /gene="menA"
                     /locus_tag="ACG808_000636"
     CDS             41950..42903
                     /gene="menA"
                     /locus_tag="ACG808_000636"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFS5046289.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
BASE COUNT        12502 a         9102 c         8107 g        13323 t
ORIGIN      
        1 aggatatcaa agattttatg aatacattta aaacaatttg aaaatttctt ctcgccgcat
       61 atactaagaa tagcgaaata tactgcctta ctttttaagg gagtgaggaa ttttgcctac
      121 aaaactttca aatgggaaat ataaaactaa tctgaggtat ccaaaaaaat tcagagaaat
      181 cactggaatt acatcagaaa agtatcaaaa agtatttcca actaggcaat tggctattaa
      241 agccgaaaat gcaatgaaaa ggaaaattga aacagtttta agagaggaga atgcaaattc
      301 actagaatta aaaggaaaaa taaaatttaa ggaattctat gaatcaaaat ggcttcctag
      361 atatgaacta ggtcagacta ctagatcaaa aagagctcca agttacgtaa caattagtaa
      421 tactaaagat atattccgat tgcacatcct tccaatgttt ggtgagtatg caatgaacta
      481 tctaaattca aatactgaga taatatcaga tgaattaaca aaaaaatcga aagaatacgc
      541 aaatataaaa attatcaaag gatatgtccg ttctatgttt gatattgcag aaatactaaa
      601 ttatattgaa tttaaccgaa ctaccaaaat tattcaatgt atcactgcac ctaaaaaaat
      661 tgctttagaa gaaagaagga tacgggaagg taatcaagca ttatcgtcag aagagcttat
      721 tgcttggata gaagcagtaa acactgactt gaataatcac ttgcttactc ttcatgacta
      781 tactcttttc atgcttaccc tatatcttgg tgatagaaaa agtgagacgt atgctttgca
      841 atggaaatat atcgactttg agaaacaaac ggttcgcctt aaacatgctt tggataaata
      901 tcaaaaaaag aaatttacaa aaggtcgaaa ggataccgta attcaagtac ccgaaattgt
      961 aatggcatta ctaagcaaat ggaaatcagt gcaagctgag caattgttga gattaaaaat
     1021 taatcaaaca ccagatcagt atctctttac ctatactaaa ccatccggag aagtaaattg
     1081 ccctgttcac gctgattacc taaactatcg aatcaattca attaaaagac gacatcctga
     1141 attggctcat ttatctccac ataagcttcg tcatacctat gcaactatag ctagacaagg
     1201 cggagcaaat atgaatcaaa tatctagtgc tttaacacac tcagatattt ctacaacgag
     1261 aatatacgta aatacaccag atgtagtaga taaaactgtt tttgaagcct ttcagagagg
     1321 attgaataaa tgtgattagt atgtgttctt aaatgtgttc ttaaacaaga aaaagacact
     1381 tttcaaacaa ttgaaaagtg ccttgaaaca ttgatatata aataatatta acgtttagag
     1441 aattgagatg ctttacgagc ctttttgaga cctggttttt tacgttcgac catacgtgcg
     1501 tcacgagtaa gtaagccagc acgtttaaga gctaaacgga agtcaggatc aacttctagc
     1561 aatgcacgag cgataccatg acggatagct cctgattgtc cagcgtatcc tccgccgttt
     1621 acgttaacaa atacgtcata tgcgcctttt gtttcagtaa cagcgaaagg ttgattgatt
     1681 acttcacgca agtcagcatg tggaatgtat tcttcaacat cttttttatt tacagtaatt
     1741 ttgccagttc ctggtactag acgtacgcga gcaactgcgt ttttacgacg gcctgtgccg
     1801 atatattgaa cttgtgccaa tgaaattccc tcctattaga ttaagtttgt gatgtctaaa
     1861 acttctggtt gttgtgctgc atgtggatgt tctgctccac cgtatacgtt caattttgta
     1921 aattgtttgc ggcctaaagt gtttttagga agcatacctt ttacagaagt ttcgatcaaa
     1981 cgacgagaat ttttagcacg caattcgcca gcagtgattg atttcaatcc tcctgggtag
     2041 ttgctgtgac ggtaataaat tttgtcagtc gcttttttac ctgttaattt cactttgtct
     2101 gcattgatga cgatgacgaa atcgccagta tccaaatgag gtgtgaaagt tggtttattt
     2161 ttaccacgta gtacagatgc aacaactgtt gaaagacgtc ccaatggaac atcagttgcg
     2221 tctactacat accatttacg ttctacttcg cctggtttgg ccatatatgt tgtacgcacg
     2281 ttattttcct ccaatttaag ttctgatgtt tgacatacac ttcgagtttc cggggctctt
     2341 tgtggggcaa acaataccat ttaacatatt acccgctaat ctagtcaatg tcaacgtttt
     2401 tctgcttgat tgaaaaattt atatgaaact ttacttcttt tctttctttt atataaaagg
     2461 gtcgtttcct gtcaaaatga aaaacaaaaa gaggctgtga caaaagtctt gtcacaaccc
     2521 cttattttga ataaacggtg ttctcctgcc aactcctcgg tcacaagtca caatatccat
     2581 aaaacataag aagctgtttt ttaaaattgt ttcttgtgac tcggagctga acggcaggct
     2641 tacaacctct tggtaaacgg tgttcaatca gctgaccctc ggtattagct caaaaaacgg
     2701 caaaatatga ggaactattt tgacgtttgt ttctctaata ctcaggtcaa aacgctgatt
     2761 tcacaacctc tttttcggtc aagttcaaca acagttttta cgcgtcgatc tctggcgctt
     2821 tgcccaaatg agcttggatc atccagatac gtttttctgt ggctgttttg aattcgataa
     2881 agatatcttg tgttggatcg tcgccttctt ctccagaaac ttcgattcct ttttggtaaa
     2941 gatctgctag ataacgatag cctgcaacaa gtgtttccaa acgttctgga atagtacgat
     3001 cccagcggcc aatttcatca gggatttttg tgttatctgc aaattcacgc aatgtagaat
     3061 aaggtgaacc gtctaatgtg atcaagcgtt cggatacttc atctaactga gcgttcaaat
     3121 catccatgaa ctcatccatc attgggtgca atgtaaggaa acctggtcct ctcatgtacc
     3181 aatgtgtttg gtgaacaaca acagacattt ggcttagatc tgcaactaat tgatttaata
     3241 cttctttggt ttgttggaat ttcatctttc ttgcctccta acattttctt acacttaaaa
     3301 taatagtaga ctttcaatga aaagtacaac aaaatcattt acctgagaac gattttcagt
     3361 tcgctttttt atccgtaaag ccaatcattt ttcagaatca gctaattctt ctcgcagttg
     3421 agcaaattgc tgataagtct gtgctaaatg cttttgtgtc gaaacaaata atggatagat
     3481 tttctgatat tttttatgat cagtttcttg tactccaact tgctctttca attctatcaa
     3541 ttcctgtgct tcactgatac tttggatctt tccgatgctt ctccagccta aaatcacagc
     3601 tcctaatgcg gatgcttctg tgacagtagg gaaaacaagc gggtaaccca gtacatcagc
     3661 catcatctga cgaaaaatcg gagattgagc aaacccgcct gtagcacgga tctctgtgag
     3721 ctgattttcc tggcaaactc cttttaaaat ccgataaagg ttgagacaga tcccttctag
     3781 aatcgccttc atcatttctt tttctgtatg ttgccgctgt aaaccgataa aactgccaaa
     3841 cgcatctgcg tcccaaagcg gtgctcgctc accaagtaaa tagggatgaa aaatcagacc
     3901 attggcaccc gctggcacaa aagcgatagc cgaaagcagt gcatcatatg gattttctcc
     3961 tttttcttta gcagattgaa cgacttcctg taaaaatgta tgccctgccc aatcaaagat
     4021 gccagcacca ttgctagtcg ctcctcccac tacccaatgt ttctgatcca gaacataaca
     4081 aaacgtctcc atttctggat ggaactgagg ctgagtagta ataaaacgaa gtgctccgct
     4141 cgttcctacg gtcaaagcgg cgatcgtatc accaaaagca cctaacccta gattagaaag
     4201 cggcccgtct gcccctccca aaacaaatga tatatcattt ggcaaaccta gttctgctgc
     4261 tgtttttgat ggtagacctg ttacttgata ggtggaagga aagacttgcg gcaattgctc
     4321 tattcgaatg ttcaagtagt caagcacttc ttctgcccat tggaattcat ggatatcaaa
     4381 atatccagtt cctgaagctg ttccgtagtc gctgatcagt tcgccaaaca atctataaaa
     4441 gatatagtct ttgataccga caaaataagc agtttttttg aataattcag gttggctgtt
     4501 ttgcagccac ttgattttgt tcaatggtgt cattggatga atcggcgtcc cagtctgccg
     4561 ataaaattct ttgccaagca cggtatattt cagtttttct gcttcgtccg ctgctcggtt
     4621 atctgcccaa gtgatcattc gagataacgg tgttttctct tcatccaaca atatcaggcc
     4681 atgcatcgca gaggagaagg agacaaattg aatgattgtg tctgaaggca actgtttaat
     4741 tacttctttt ataacggtgc agacaccttg aaagatcaat tcctgatctt gttctgccat
     4801 cccttgtgtt tcttggatca aagggtagcg aaaataagcg gatgctgctt cttgggcttc
     4861 atctgtgaaa gcaactgctt tggtttgtgt ggttccgata tctaatccga ttgaaacgtt
     4921 cattttttca cctcaaattt tatgtatgta caaaaattta gtttaagctt gcgtatcttg
     4981 gttttgaaaa gggggagatg gcgtgttact ttattttatc attggttgct gtatcggatc
     5041 atttttatgt ttaaccgccc agcggattcc gttaggtcac tctattattt atcctagatc
     5101 tcattgtgtc aaatgctgtc gttcgctttc ttggtatgaa ttgattccga ttctctcgat
     5161 catcgtccaa cgatttcgct gccgctattg caggtgtcgg ttgcccttct actatttgtt
     5221 ggcagaagcc ttatgtggtg gtctttttgc ctggttcttt actttttctt ccgcaagaaa
     5281 tttttcgacg ttcatatgga tgctttcggc tttgctattt tcgttgatgg atttatttta
     5341 cttcatggtc cattctcaca cgctattctg ttcttgggca attttatgga ttttttggtt
     5401 gcaggccggc gtcttccaat ggcaaagtgt gctgttaacg tttatcgtag gcggtgtgtg
     5461 ccttcgctat ggtcaatcct atctcggagc tggcgatact ctgttgatgc tttcttggag
     5521 tggtggcttg tctctagaag agttgcttca gatcttgttt cttgctagtc tgtttggaat
     5581 atccgttttt tctgtttact ttatctgtca taaaaaaaag ttggaaaagc tgcctttcat
     5641 tccttttttg agcagtgcgt tgctgattgt ccttcatctt aggtattgaa aaaaggttgt
     5701 gacaaacgac ttgtcacaac ctctgatata tagtgttctc ttaccaaatc cttgtgactt
     5761 ggagctaggc gacaggttca caacctctcc actaaatggt gttcaatcag ctgaccctca
     5821 gtattagctc aaaatgctgg ttttacaacc tctgatatac ggtgttccat aagtagcttt
     5881 ttcggcttct tatttcttga cgccccaatg tcgacgaagg aaacctgcac gtccagaacc
     5941 ttctcgataa agattgtaat ggagcttgtt tttcttgtaa taatcctgat ggtaatcttc
     6001 tgcaggataa aattcgcttc ttggttcgat ttttgtcacg attggcttat cgaatcgccc
     6061 gctgtcttgc aatgcttgtt ttgaacgctc tgcaatattt cgttcttctt catcattata
     6121 gtagatcact ggacgataag aatcaccgcg atcagcgaac tgtcctaatg cgtcagtcgg
     6181 atcggtttgc tgccaataga tttccaccaa ctgatcataa gaaataactt ctggatcata
     6241 ttcgatctct actgcttctg tatgccctgt tgtgcctgtt gttacttgtt cataagttgg
     6301 atttggtaca tgcccgccag tataaccgga tgtcacagaa atgatccctg gctgctcttc
     6361 aaatggtttg accatacacc aaaagcatcc gcctgcgaaa actgcttttt ttgtcatata
     6421 aaatccccct tttttctcat cataccttga tttccaaaag gaggataacg ttttgcttgt
     6481 gacagaaaaa acggaaacta ttatatacgt gatacggctt ctgataaatg ccaaaacgaa
     6541 cgtacttgat caaaaacggt taaatcagga atcacatatt cagcagaagg aagatttatg
     6601 ttttcaccta ttcccaatac atgcatgtta ccagccaaag ctgcttgaca accagcttct
     6661 gaatcttcaa tgaccaagca tgcatttggc ggtacattca attgctgtgc tcctttgaga
     6721 aaaacttctg gatcaggttt tgcttttgat acttgcgtgc cgtcaataag gacatcaaaa
     6781 taacttgtca aattcgtttt ttctaaaatg aggcgtgcat ttttgcttgc tgatcccaat
     6841 ccgatcttga tgtttgtttt tttcaagtac tcaagaacag ctattgcacc aggcaaaacg
     6901 gatgtttcat ccattttttg aatcgcttga agatatagat tgtttttttg ctcagcaaag
     6961 gctgatttct ctttttctga gaatttctgt tccatttttc caaatgacag caatcgctct
     7021 agtgactcgg ttctggatat tcctttcagt tgctcgtttt ctttctcagt gaatggaatg
     7081 gacagctggt tagctaaaac cagccacgct tcgtaatgat aacgagctgt gtctacaaga
     7141 acgccatcta aatcgaatag tgctgctttg attttcatcg attacctcct tgaaaatgaa
     7201 cgacttgaag taaagatgcg atgtttttag ctgtgcgatg aagattatca gtggcttgct
     7261 gtaaagcttc ttgtaaaggc acagctgcag gaaggatcgg aaaaacggcc gaaatgccaa
     7321 catctgctgc tttttcgctt cctttaccaa cacttccgca aatagctatc acaggaacgc
     7381 cctctggtac tcttctagca atcccaactg gcgcttttcc ttttagactt tgtccatcta
     7441 gacgtccttc gcctacgatt acgaggtctg cttgctgtac ccgtttatcg aactccagta
     7501 aatccaatac gtagtcgatg ccagagacta ttttccctga agcaaatgct aacagtcctg
     7561 cacccattcc accaccagca ctactgcccg atgcctcgat tacttgaggg gcaaatctca
     7621 tataaaaatc atgcatggct tggtcagcca ctgctaattc attttctttc aagccttttt
     7681 gtggtccgaa aacaaaagaa gcgccttctt ttccgcataa aagattagta acatcagtaa
     7741 tgacagtgat tttggcttta atcagctcaa taggcacgtt cgttttatca actgtagcaa
     7801 ttttacctag attttctccc agtgcttcta ctttttgatt atcttgatcg taaaaagcat
     7861 aacctagacc ttctgccatg ccgatgccgc catcgttggt actacttccg ccaacgccga
     7921 tgatgatgtg tcgaacacct tgttgcaaca attgtcgtat cagttctcct actcctcgcg
     7981 tggataacct aagtggctgt tgttggttta gtgggacatc tttcaatccg acgatttctg
     8041 ccatttcaaa aatcgcagtt tgaccgtctg aagcgtactg gatttctttg tttttgccaa
     8101 atgcattagt aacgactgct gtttgccgct tcaaccttaa actgtctgct aatgatgaaa
     8161 ccgttccttc tcctccatcg cctataggta gcagatcata ttcagcctga acaaaaactt
     8221 cttgaaaacc tgattgaatg gcttgtgctg cctctaaggc tgtcagactt tctttgaaag
     8281 aatctggcgc aatcagaata cgcattattt tcccctccgt tccaagatca gttctttttc
     8341 cagctggtat tggtctccat tgacaaaaat aggtaatgtt tctttcgtct ctttttttat
     8401 tactatttgt ttttttgtca aaacaaatag caacctgtga tttttccaaa taaaaggaaa
     8461 agaaattttt tcccatgctg aaggaagtct tggagaaata gaaagagttc cattcgttac
     8521 tgtaatacct gaaaaaccaa atataactgc tagccatagt gctcctaatg aagcagcatg
     8581 aattccgtca tctgatgaat gcggatctgg tcctaaatca atcaggcacg cttcttggaa
     8641 taaatgatac gcccattcca tttcttggat acggcaagcc tctatggcgt gaatcgcttt
     8701 actcaacgat gagtcgtgga ttgttttgtt ttcatagtaa tgcaaattct tagaaacgat
     8761 tggttgagaa aaatactcag gtaataagta taacagcatg acaagatctg cttgcttgct
     8821 gacttggagt tcatttactt cgctacgggc ataatcaagt aaaatagact ggctgccagg
     8881 acgttctttg taagtggtca aatcaataac tggcttactg agatacgtgt catcttgcgg
     8941 aatcagctgt tctttgttag gcactggcaa atataactta tctaaaaaat tttgacattc
     9001 tttcaaaaaa agcggatcgc cagcttgata tttaacagct aaggccacat tataatgcgc
     9061 cagatagttc gtatatgcat tattgtcgat atattctgtg tattcatctg gaccaatgac
     9121 tctgtgtatc tctaaatgat catcgacttt tacagctctg ctcagccaaa aagctgctgt
     9181 ttcatgaatg agcgtttttc cctttgtttc taaaaacgat tgatcctctg ttgcttggac
     9241 atactcttga acggcataag caaggtctgc actgatatga tgttctgcgg cagctgaagc
     9301 aacttcttgt cttgttccgg ttttgatatt caatgcagca aatttgggtg tctcttcctg
     9361 accagtgtgt gcgctttccc atgggaaaag tgcaccttga tagtgataat ctcttgcttt
     9421 ttgccgtgcg ccttctaatc gatcataacg gtacagcagc aaattctttg ctacttcagg
     9481 aaactgatat aaaaagaaag gcaggataaa aatttccgta tcccagaaaa catgcccttt
     9541 ataaccttcg ccagttagtc ccttcgcccc tacactaaca cttggatcat tgcggggagt
     9601 cataatctgt aaatggtaag cagcgaagtc catcgctaac tgatcgaaag aagaatcgcc
     9661 ttggatcgtt atccgatgct gatgccaaaa atctgtccaa taagacatcg actgctctaa
     9721 aatttctgta taactaacgc ttttctgggc ttgggcaata cttttcttaa acgggtctgt
     9781 ttgctgaaga gaggtagaga gcacgatgat cttctccagc gtaaaggacg tattttttct
     9841 gatcttttct gaaaaggaag cggtgatccg gcgatttttt gcttggaata cacctgcttt
     9901 attgaattta gctgcaatcc caatatcaat tttactttct gttgtctggt aggatgcact
     9961 gatcaaatcg tcaccaaata ctcgcagttc tttttcaacc aactgctgtg taccaaaatt
    10021 ggtttgctgt ccatcgatac ccgttttcac atctacttgg cagtcttcgg tcaacggctt
    10081 gatgaccact ttcagggctg cctgatggag attattttga tcagcaacac gacaagtaat
    10141 gacttccact ttttttcctt gagcagtttc aatgagcgca tagcgtttaa gctctcccat
    10201 ttctaaatct agtgcccgct cgtatgcaag aactttcatc gtctcaagag aaaaatgttc
    10261 accgtcgatt tttatttccc atctgatgac atctgggaga ttcactaact cggctgattc
    10321 tgctcctaaa ggtcggttat atacaccagc tgcaaacatt ccccttactt gattccgata
    10381 gtcttcctcc tggcttcctc tgataccaaa aaatccattt ccaatggtca tctgactcgc
    10441 atacttattg atatactcta aatcaaactg tttttcttct actttcataa ggtaataact
    10501 tctcctgttt ctgccgaacg atataaattt tccaccagat tctggatgac atatccttgc
    10561 cagccatctg caatcatctg agtctggtca ccgagcgccc gattcacaaa atgagccata
    10621 cttagttcat gtgggtcatt aactggcgtg gtcttttgta aggaaaccag ctttccatct
    10681 acatcttcga atagttcgcc tggatacaga ttagcacctg cctgatcgcc acaaaaagcc
    10741 acattcagtt tgtcttctct gatattcaaa gcaaacgaag tattcagttg gatgatcttg
    10801 ccgttttcta attccagtgt gccaaaaatt gcatcttcta ctgtgaattt gcttggatcc
    10861 catttcccga atttccctga attttttttc gtaccaattt tttggaagct gtacgcccga
    10921 acgcttttta ctttaggaaa tccagtaata tacatcgctg tatcaagcat gtgaatgcca
    10981 taatcgatca aaggcccgcc tccttgaagt tctttgtcga taaacgttcc ccagcctgga
    11041 acaccagacc gacgaatcgc attcgcttct acgtaataaa tatccccgag aacatcaaga
    11101 tgattcttga tcattgttgc ttcgtcagaa aaacgatgct ggaatccata agccaaaatc
    11161 tgcccgtttt tatcggctgt ctctgccatc tttcttgctt gatcagctgt catagctggt
    11221 ggtttctcgc acatcactga agcattagct tcgagggcag ccaatacatg ttcgaagtga
    11281 aagcgattgg gtgtacagac actcacgata tcgggagaaa tctcattcag catttgtgta
    11341 actgatgtat acgcatgaga aaatcctgta cgtcggcaaa agtcttctgc tcgctggctg
    11401 actggatcaa ccacagcggc caattctaag acatctgatc ttttttgata ataagcagca
    11461 tgtacttttt ctgctacttg tccagcgcct atgattgcta ctttcttcat ttatttctcc
    11521 ctcctttgct tatttactta ttgttattct ttctctattc tttctctaaa tcatttaaat
    11581 gacgcgctgt ttccagacag tttttcaagt attgcaatga ttcctgataa attttcactt
    11641 cattttctcc tcgcaaacga cattcatata ccatataacc ttcatactca ttttgaagta
    11701 gcgtttgaaa atggcgagta aaatcaattg cgccagatcc tggttggtag cgatgattat
    11761 ctgctaaatg gatatgaccc acatactctt tttgcgtagt caatgctttt gaaatatcat
    11821 cttcttcaat attcatatga taaaaatcag cgatgatctg cacatgtttc aattgattag
    11881 agtcaatgta atgacgcgca tccgctaaca aattgatcat atggtcttgg tatcggttca
    11941 aaggctccaa ataaataacc gtacctgttt cttcggctac tttatctaaa taacgaagtg
    12001 attcgctgac tgctttatag tctccttctt tgcttcgggg tgaaaccatc ggcggtaatc
    12061 gataagtgaa cattccccaa gctgctggga taacgattcc tttgcctcca acctcgctta
    12121 gtgcacgcaa aatctcagca atttgacgta caccattaag acgccgttct tcgataaagt
    12181 caccgatcca cccatcgtag ccgttacaag ctgtcactac tgggatccct gtttctttga
    12241 ttgcctgttt gactgtgtcg atattttcta ttaacaactt accgtcgatt tcgtattctt
    12301 caaatccaca gtctttgata aatctaaatt tttctaaaat gtctcttggg aaaaatgctt
    12361 gatcttgcgt accgaatttc attgttctcc tcctcggact tgaacgccca ttttgatact
    12421 ttcttctggg tgctggtcta cataacgcat gtaagcttct gcgctttctg caaatggcac
    12481 aactggatca atgatttctt cacaatccaa gtaaccgttc atcaataatt cccaacatgt
    12541 gtcctcgatt cgtttacggt tccatcttgg gtaatctggg tttggttcac tagatgctcg
    12601 agcaaagacg atttttgcat tattgaaatg tgcttcgcga ccaagattaa acccagctgg
    12661 aaaaggttta gcaaaggcaa cataagaaat gatcccgcca tagccgatcc ctcttaaaga
    12721 agcttgtaat gcggcagcac tgccactcgt ttcaataact gaatctactc cacgtttatc
    12781 tgtcaattcc tttagacgaa aaccgacatc tgctttcata ggatctagcg tgtaatcggc
    12841 accgtatttt tctgctaatt ctctacgctg cgctatggga tcgatcccaa cgatggtagt
    12901 agcacctgct tttttagcta attgaatcgc aatttgcccg atagcgccta aaccgaagat
    12961 tgctacagca tcacctgctc gaacatgtgc ttcccggata ccacctaaag cgaattgagc
    13021 aggatcgtaa catactgcat ttttccattg atccaaccga tcaagtttac gtagtttata
    13081 attatcaaca ccgtttgtaa tcagcgtttc acgaatgggt ccataagtgc agactaactc
    13141 tcctttttga tactctgtca ccaaactgcc cgtttcttcg atcgtaccga caatcatatt
    13201 tcctaattga aattcgccaa ataccacacc gcgttggctt ccttcttctc ttggcaagaa
    13261 taggcgccat tcttctgaaa attcttcatc gataaatgga cttaatcctc taaagtcgac
    13321 aatttctgtt cctggttttg gagtagcgaa aattactcgg atacgtactt cgttcttttc
    13381 gatctcacgg tcttcgtaat ctttgaatcc ggctattctt ggttctaatg caactaattt
    13441 tttcatcttt tctttcctct ttcttttctt tttatccttt tacgccgcct tcgactaaat
    13501 cacctttaat aaatcgttca gataacgcat acataatgat gactggtaaa gcggtcacca
    13561 atgaagcggc catcattcgt ccccaaatgt agtctggtgt actgaataat gtgttcaaac
    13621 cgattggtaa agtaaatttt tcggaggtcg acaggaaaat cgaagcaaat aaataatcat
    13681 tccacgaaac cataaagcaa taaacgaaga cagaaatgat tccagaaata gccaatggta
    13741 taatgatccg aataatgatc tggatccggc taagaccatc gatcattgct gcttcttcaa
    13801 tatcagtcgg aatcgtgtca aaataacttt ttagcatgaa tatcgcagta ggaagtgttt
    13861 gaacgaccat cgtgataatc agtgctgtat tggtatcata caaacctaga ccggaaatga
    13921 ttttaaataa tggaacaacg atcaaaatac cggaaaacat ataaacggtg taaaagctag
    13981 cattgatttt cgagcgagct ttgaagcgta gttttgacaa agcgtatgcc ccgctgatcc
    14041 caatcactac tgcagttacg gaggctatca aggagatcac taaactattt ttgaaataca
    14101 tgatgaatgg aaaaatctca gcattgaaaa tatcctgata atgttcaaaa gtccattctt
    14161 tcggaaaaat agtttgtgtc gttgcaattg cttcttgtga atccttgaat gatgtcatca
    14221 gcatgatcaa aaatggaaac aaattgacag ctaatagcaa caaaatcgtc gcgtaaaata
    14281 agattttcct gctcttttta tttttcattt caagtccacc ttctttcgtg agagcaagac
    14341 gaccgctgca ataatgatga acaatagaat cgatatagct gctgcttttc caagatcatt
    14401 gaacgcaaag gctgtctgat acaaataaac gcctaagata ttgactttat tcgtcagtag
    14461 atagacatct gtaaacatat agaacatcca gatagatcgt aaaacaatca cagttgctag
    14521 tactggtttg attgccggca gtgtcacgat cttgaacttc tgccaagcat ttgcaccgtc
    14581 catttcagct gcttcataaa gcgttttgtc gactgtttgc aaaatagcca aaaatgaaat
    14641 aaacgcatac ggatagtatc tccagatagc aaacaaaacg accataaaga agctagcaac
    14701 tggctggtcg aaccacagcg gtgcttcgtt gaaaagctgc aacctatcta ctaacagatc
    14761 attgacgatt ccataactat tattgaacat atatttccaa gcaaaaatca aagaaataga
    14821 tggcgtaaca taacttaata aaatcagtga acgcaccgtt ttcctaaatc tgaacttgcg
    14881 gttaagcaaa atagctacgg ctaatcctaa agctgtactg cctgctacaa ctaagaatgt
    14941 gtaagcaact gttaaaccta aagaacgata aaattcacta tcacttaaaa tttcaagata
    15001 attgcttaat ccgataaaaa cggcgtctga gttaggattc agcggcatat cgaaaaaact
    15061 gatctgaata ttggaaagca ttggaatcag cactaaaaca gctaataaaa gcatgcttgg
    15121 tgctaaaagt cccatggcta attttaagtc agaatgtttc cttggacgat tcattgaaac
    15181 ctccccctga taatctgcca atagctatct acttttttcg aacaccattg gcagaacttc
    15241 ttgttatttc gtattttttt gtgcgtcagc taaagcttga tcgacatctt cgtttccgac
    15301 agttacttgg ttcactgctt ttccgatcac acctgagctt gtgatatctc ccattttcgt
    15361 gaaattcttt tccccgacta gtccaaagac ttgtacttca tcaaaagcac tagcaatctc
    15421 agatggcagc tcgccaaaag acttgatgac ttcattttct ttgtaaccat ccagttcgac
    15481 tacttgttta ttcactggtt gagcaccacc aggagacatc aagacccact tttccatgtt
    15541 tttcggttga gataagaaag ctgtgaatgc ttcagcagcc tctttttgtt tctcgtctag
    15601 accagaagaa atcgtcaatc cagatactgt gccatagaca gcttgatctt tttgtgttgg
    15661 gatagcaaaa ccgatatttt ctgagttccc cgcttcataa acagaaggca aaatgtaagt
    15721 tgaatagatt gccattggtg ctgttccatt catgaaagca tctttgattt ctgtcacatc
    15781 attagagcct ggcatcgtgt attgggataa gttttgataa aacgacaatg cttctctcat
    15841 cttttccgta tcgatcgtta cctcgccttc atcatccaaa acatttgctc catttgacaa
    15901 agcaaattga gagaacgctt gctcactcat tgttccttca gcagtaggca tagcaattcc
    15961 atattgttta ttctctttat ctgtgaaatg ttgagcaatt tttaaaatat catcccaatt
    16021 ttctggttct gaaaaaccag cttctgataa tttttctttg tcataccaaa taccttgaac
    16081 ccagccgcta attggtgcag caatatagct ttttccatct tctgaacgaa caaggttttt
    16141 ggctccgtca taataattat cttctccagc ttcctcgata acgttttgga ctgccgtctg
    16201 atcaatcagt tcatctttat ccatcacttt agcaaaatcc tgactgactt ccattacttc
    16261 tggtaattta ccggaacgag ctaacgtaac gatttttgta ttgaacgctg attcttctac
    16321 cggcacttgc ttgattttga tgtctggatt ttctttttca aaatcagcaa ccagttcatt
    16381 gatcacactt aatcgttcct gttcgacaga agagtgcatg aattgaatcg ttgtctgttg
    16441 tgattcgtta gagctttgac tttcaccaca agcagtaaga agaaatccag tagctagcag
    16501 acaggtagta acaataattt ttttcatttt tttccacctc ttattttatt attttcagcc
    16561 acatatattc atacggctgg aatatcaact ttcgttcttg actgcaatgg ataatttgtc
    16621 cagaaaacag gtctaggtaa tctccttttg gtaaaagata acctgctgat tgattggaaa
    16681 aattatggat aactaagact ttctcttcct tcgcttcgcg aataaaactg ataaaagaat
    16741 ctgctgagtc cagtaagtaa atcaaactat tcggatggaa ggctttttct ttttttcgaa
    16801 tagatagcat ctgggtcaat tcaccatata ctgctgttct taatggatct gtctgcaact
    16861 ctttctcaat atctgctaac aaaaattttt gccgattgat ttttcgattc tctcctgttt
    16921 tttctacgcc tttgtaatca tttcgacttc ccagcacact ttgaatataa attgccggca
    16981 cgcctggtaa cgttaccaaa aatgaatgag cagctaaaaa gcgtttgctt ctccattccg
    17041 tcgtatctgt tggagaactt aacgcatcta gatacgtagc gtttatctca tatggactgg
    17101 tcgttccatc tgggtttttc ttgtagttta ctcgggcacc ctctttcttc agctgttcca
    17161 ctaattcaag gatcgttttt tccggcagaa tacctcgtac cggattcaat ccgataccgt
    17221 catgggaggc taaaaaatta aaaaaagttg tttttttcga tgcgaaatcg atggttttta
    17281 accattcttt caacttactt gtattacccg aacgaatcgc ttgaagggtc aaaggtggta
    17341 gcgaaaactg atagaccatc tgtgcttcat tctcgccatt cccaaaataa ctgatattat
    17401 ctttgtgtgg cacatttgtc tcagtgatca ataccgttcc tggagctgct tcatcagcga
    17461 ttgcacggaa taattggatg attttatgcg tttctggcag gtgtatacaa gaagttccta
    17521 gttctttcca taaaaatccc acagcgtcca agcgaataaa tgaagcgcct ttttctaagt
    17581 aaaacaaaag aatatcgatc attttcaata aaacttctgg atttttaaaa ttcaagtcta
    17641 cttgatcttc gctaaaagtc gtccatacgt gtttttcttt cccgtttttg tcaatgaacg
    17701 ttgtaagtaa cgggctggtc cttggtctag tcacgctgct tgtatctgta tccagtggca
    17761 aatcaataaa aaaatctttg tacgtatcat tcaagtttaa atattcctga aaccaagtac
    17821 tttcggcaga catatgattg cagacaaagt cgaacattaa ccgtgtttcg cttctcattt
    17881 cttctatatc ttcccatgtg cccaaacggt cgttgattgc ctgataattt acaacggaga
    17941 aaccatcatc agatgtatat gggaaaaatg gcaagaaatg tacaactcta aatgtatctg
    18001 ccaaatactt ttgataaaat ttcttaaatg tacgcaaagt tggttgctgc tcttcaaaaa
    18061 attgatcccc ataagtaatc aagaaaatat cttcttcgtc ccaatgcagt ttgcctgcct
    18121 ggcctttctt ctgctgataa acactgactt tttgttcaat catagaggcg gttttctcag
    18181 acaaatctga gtcgcgataa atcgcttgta aaagttttgt atacatcgga ttcccctttc
    18241 ttttttggga gcgttcccaa cgatttgatc atattatgga aaacgcttac tgtcaatagc
    18301 atttttttaa ataattttga tatacacaaa aatacaaata agaaagctta tgtatcaata
    18361 attaatagct tcttaaaaaa atatggtctc tcatgatcat gcaagctatt cctaaatttt
    18421 caaaatcata gacaaaaaag aatgtctatt gtactatgga attattctaa aacgctccca
    18481 aaaaggaggt gcatataaaa ttgaaaccta ctatttatga cattgcgaaa aaagctaatg
    18541 tctcaaaatc aactgtctcc agagtattaa ataaccagcc aaatatctct gataaagcaa
    18601 aagcaagtgt acttcaagca atcaaagaat tgaattatga accgagcaaa attgcaagag
    18661 gactgtcagg aaatggtttt gacgcgattc tagcattatc tcatcgtact tctcatacga
    18721 cctatggcaa tccttttttt tcagatgtta ttcaatccat ttctgccgtt tctgaacaaa
    18781 ataacttcga cttgattcta caaacggcaa aaacaccaga agaagagcta gaaaaatgcg
    18841 cgaaaaagat ccaagaaaaa atgatccgag gaattattat tttaagctct acgatggatg
    18901 aaacacttct ttttgaattg gatcaataca atattcctat tgtggtaatc ggaaaagtcg
    18961 gaaacgacta cacgaacatc tactcagtgg acacggataa tttccaggat agttacgatt
    19021 tggtggaaac actgatcaag aatggacacc gtcatatcgg ctgtctccat gctccgacag
    19081 aagtttatgt atccaatgat cgtctcaatg gttaccgtca atgcttattt gatcaccacc
    19141 tagatatccg taatgaatgg attatagacg gcggctttcg cttagaagat agcatggcag
    19201 caatgaatga tttaatgaag ctacgagaaa gaccaactgc gattttttct accgatgcat
    19261 taaaatcatt gagtatttac aaagtggcag aacaatctgg aaagcagata cctgatgatc
    19321 tttcgatcgc aggattcagt gatcccttat tgtcctccct attttcgcca cagttgactc
    19381 ggatagacat tccgacaaat cagttaggaa tcaaagccac agaactttta ttccaactaa
    19441 tccaccacca ataccctgaa gaaaaaagaa tcattctttc aaccgagatt actctcacag
    19501 attcgatcca acaacaagaa atgtagaaca gaattataaa agaacagaaa cactgaggat
    19561 aagtcagtta ctctgttctt ttttcaatca ctcatccctc gttaccccct cccggtattt
    19621 tagcgaattt ttcttatttt ccatctgttt tagatagctg tggataacta taatgtttgt
    19681 gttaaactat tttttccaca gggaagaaaa aactattttt aaccacgttg tgtataagtt
    19741 tttatagttc gtttttttaa acacatttac ccttatttca atccatgtta tttggggata
    19801 cttatctcta aaacataaat agaataacag acatgtctac tggttttgtt ttattcgagg
    19861 aattattcaa taatagctag caaaatatta aaaataaccg ctctatcttc ccatcaattt
    19921 attttgcatt caaaaaatga tctatcttta cttttgttga ttgacttctc ttggaagatg
    19981 atcgttccga taccttaatt ggatacgaac cgatgttgtt aagatccttt cccacttttc
    20041 ttctagttag acaaattatt ttagacaaac caaaaaagag gttgtgacaa taatttgtca
    20101 caacctcttt tttaaaatat ctattttgtt tgattgttca ttgcgtcttc tgcagccatt
    20161 tgcgcttcaa tatctgaaat actgtagaca ttttcgcttg ctacaccgac ttcttttggt
    20221 tccatgtcac gataacgttg cataccagta ccagctggga tgatcttacc tataataaca
    20281 ttttctttca aaccaagaag aggatctttc ttaccgcgga tcgcagcatc cgtcaagaca
    20341 cgagttgttt cttggaatga tgcagcagat aagaaactat ttgtttctaa tgatgctttt
    20401 gtgataccta gtaagacagg tcgagaagtt gcaggaacgc ctcctgagac tagcgtattg
    20461 tagttttgat ctttaaagtc tgcgatgtct aataatgtac ctggtaagat atctgtatca
    20521 cctggatcca ttacacgaac tttacgcagc atttgacgaa ccattacttc gatatgcttg
    20581 tcgccgattt ctaccccttg catacggtaa acacgttgta cttcacgaag caagtagttt
    20641 tcaacagata atacatcgcg aacttgcaac aattgttttg gatcgattga tccttctgtc
    20701 aatggtgcac cacggtggat catatcgcct tctgctactt tcatacgtgc tgtataagga
    20761 acagtatacg tacgtgtatc tgtttttcct ttgattgtta cttctttggt acgtgttgca
    20821 ggatcttctg agatatcgat gacatcccct gttacttctg tgatcactgc ttgccctttc
    20881 ggattacgtg cttcaaagat ttcttggaca cgaggaagac cttgagtgat atcgtctccg
    20941 gcaaccccac ctgtatggaa tgtacgcatc gtcaactgag taccaggttc accgattgat
    21001 tgagcggcga tcgtaccaac tgcttctccg acttcaacgt cagagccagt tgcaaggttg
    21061 cgtccgtaac aatgtttgca gacaccatgt tttgtattac atgtgaagac agaacggatt
    21121 gtcactgttt cgacacctgc atcaacgatt tgttttgcga tgtcttcgga aatcaattgg
    21181 tttcctgcaa tgatcaattc atttgtttca ggatgacgta cttctttacg ggtgtaacgt
    21241 cccactaaac gatcttctaa tgattcgatg atttcatttc cctcgcggat ggcttggatt
    21301 tcaagacctc ggtcagtacc gcaatcttct tcacggatga ttacatcttg ggccacgtca
    21361 accagacgac gagtcaagta acctgaatcg gcagtcttca aggctgtatc ggtcatccct
    21421 ttacgagccc cgtgagttga gataaacatt tccaagacgg atagtccttc acggaagttc
    21481 gaaatgattg gcaattccat gattcgacca ttaggagcgg ccatcaaacc acgcattccg
    21541 gcaagctgtg taaagttgga aatgttacca cgagctccag aatcagacat catgaagatt
    21601 ggatttttcg cttccaaacc ttccatcagt ttttgttgga tgctgtcttt tgcagcattc
    21661 caaacagcaa tgacacgttc ataacgttcg tcatctgtga tcagaccacg acggaattgt
    21721 tttgtaatag tttcaacttg tttatgggca ttttcaatga tttcttgttt ttcattcagt
    21781 acactgatgt cagcgatccc tactgtgata ccggcatgtg tcgaatgttt gtaccctaag
    21841 tctttcatac ggtcaagcat cttagaagtt tctgttactt tgaatcgttt gaagacttct
    21901 gcgatgatgt ttcctaagtt tttcttcttg aacggtaata ctaattcttg ctctttgata
    21961 tgtgcaggaa tatcagtacc agcttcaaca aagtatttgt ccggtgtttg gacggtcaag
    22021 ttgaagtctg ttggttcatt cagataaggg aattcaggag gcatgatctc gttgaagatg
    22081 atcttaccaa ctgttgtgat catcgtacgt tctttttgcc attcagtaaa tggtttttca
    22141 cctaatgagt tagggttcac accgatacgt gaatgtaaat gaacatatcc gttgcgccat
    22201 gcgataacgg cttcgttttg atctctgaag accattcctt cgccttcgcg tccttcttct
    22261 tccattgtca agtagtagtt acctagaacc atatcttgag aaggagtaac aactggttta
    22321 ccatctttag ggttcaagat gttttgagca gctaacatca acatacgtgc ttctgcttgt
    22381 gcttcttcat tcaatggaac gtggacagcc atttggtctc catcgaaatc ggcattataa
    22441 gcttcacata caagtggatg caaacggatt gcgcgtcctt gtactaaaac tggttcgaat
    22501 gcttggatac caagtcggtg aagcgtaggt gcccggttca gcaatactgg gtgttccttg
    22561 attacgtctt ctaaaacatc ccatacttcg tcttcttgac gttcgatttt acgtttcgca
    22621 tttttgatgt ttgaagctag ttcgcgttga acaagctcac gcatcacgaa tggtttgaat
    22681 aattcaatgg ccatttcttt tggaagtcca cattggtaca tcttcaagaa aggtccaaca
    22741 acgataacgg aacgacctga gtagtcgaca cgtttaccta gcaagttttg acggaaacga
    22801 ccttgtttcc ctttcaacat atgagaaaga gatttcaatg ggcggttacc tggtccagta
    22861 actggacgtc cgcgacgacc gttatcgatc aatgcatcta ctgcttcttg cagcatacgt
    22921 ttttcatttt gaacgatgat tccaggtgca ttcaagtcta acaaacgttt caaacggtta
    22981 ttacggttga ttacacgacg gtaaagatca ttcaagtcac ttgttgcaaa acgtccacct
    23041 tctaattgaa ccattgggcg taaatctggt ggaataacag ggataacgtc cataaccatc
    23101 cagcttggtt gatttcctga agcgcggaat gcttctaaaa tatccaaacg gcggattgca
    23161 cgtgtacgtt tttgtccttg tgcagatttc aattcttctt tcaattcggc tgcttctgca
    23221 tctagatcaa cattatcaag taattgttta atcgcttctg cacccatagc agcttggaat
    23281 tcttgtccat attgttcacg tttttcgcga tattcgcgtt ctgtcaacaa ttgttttttc
    23341 tctaaattcg tatttcctgg ttcgatgact acatatgaag caaaataaat gatttcttcc
    23401 aaagcgcgtg ggctcatgtc taatacaaga cccatacgtg aaggaatccc tttaaagtac
    23461 cagatatgtg aaacaggagc tgctaattca atatgtccca tgcgttcacg gcgaactttc
    23521 gaacgagtaa cttccactcc gcatcgatca caaacgattc ctttataacg gatacgtttg
    23581 tattttccgc aggcacattc ccagtctttt gtaggaccaa aaatccgctc acagaacaac
    23641 ccttcgcgtt caggttttaa ggtacgatag ttaatcgtct caggtttttt tacttcaccg
    23701 taagaccagc ttctgatttt ttcgggagaa gccaaaccta tttgcatact ttcgaattta
    23761 tttacatcga tcaaaagggg ttccctccat ttcatttaat ggcaaaccgc aagacggctg
    23821 atcttcgacc gccttgcagc tcatagaccg gattagtcta aattatcttc tgcggtttca
    23881 aagttttgga tgacatcgtc tttttcatct tccacttgtt cagcagcttt cttttctaat
    23941 tctttggcag tttgctgttc ggcaaatttt gtcaaagcat caacagtgat caaatcgtcg
    24001 tcttcatcgt ccatgtcgcg aagttcgatc tctgtttctt cgatatccaa gacacgcatg
    24061 tccagaccta gtgattgcaa ttctttcacc aatacgcgga atgattccgg tacacctggt
    24121 tttggaattg gttcaccctt gacgattgct tcgtatgttt tcacacgtcc gactacgtca
    24181 tctgatttgt atgtcaagat ttcttgtaaa gtataagccg caccgtaagc ttccagtgcc
    24241 catacttcca tttcaccaaa acgttgtcca ccaaattgag ctttacctcc caatggttgt
    24301 tgtgtaacca atgagtaagg tccgattgaa cgtgcatgca atttgtcatc gaccatgtga
    24361 gcaagtttga tcatgtacat cacaccgaca gacacacgtc catcaaatgg ttcacctgta
    24421 cgtccgtcgt acagaacagt tttcgcatcg ctggccatac cggcttcacg aactgtttcc
    24481 catacgtctt catcacttgc accatcaaat actggcgttg ctacgtggat acctaactga
    24541 cgtgcagcca tacccaagtg taattccaat acttgcccga tgttcatacg tgaaggcacc
    24601 cctaaaggat tcagcatgat atcgattggt gttccatcag gtaggaatgg catatcttct
    24661 tccggcataa tacgggatac aaccccttta tttccgtgac gaccggccat tttatcccct
    24721 tcatggattt tacgtttttg aacgatataa acacgaacta acatgtttac gcctggtgac
    24781 aattcatctc cagcttcacg agtaaagatt ttcacatcgt gaacgatacc gccgccgcca
    24841 tgtggtacac gtaatgatgt atcgcggact tcacgagctt tttcgccaaa gatcgcatgc
    24901 aatagacgtt cttctgcaga taattctgtt accccttttg gcgtgacttt ccctacaagc
    24961 aaatcgccat cttggacttc tgcaccaata cggatgatac ccatttcgtc taaatctttt
    25021 aatgcgtctt ccccgacatt agggatttcg cgtgtgattt cttcaggtcc taattttgta
    25081 tcgcgggctt ctgattcata ttcttcaata tggatagaag tgtatacgtc atcttttacc
    25141 aaacgacggc tcatgatgat cgcatcttca tagttgtacc cttcccaagt catgaaacca
    25201 actaaaacgt tttggcctag agccatttcg ccttgttcca tagatggtcc gtctgccaga
    25261 gtatcccctt tttcgacttt ctcacccaaa tgaacgattg gacgttggtt gtagcttgtt
    25321 ccggagtttg aacgacggaa ttttgttaca tcgtatttat ctaatgcacc gttgtcgcgg
    25381 cgtacgcgga tttcagaagc atcaacgtat tcaacgacac cgtcatgttt acataacaaa
    25441 gcagctcctg agtcatgcgc tgatttgtat tccataccag taccaaccca tggtgcttga
    25501 ggattgatca acggcactgc ttgacgctgc atgttggcac ccatcaaggc acggttggag
    25561 tcatcgtttt ccaagaaagg aatacatgct gtcgcaacgg caactacttg tttaggtgat
    25621 acgtccatgt agtctacttt atcaatagat acttctaagt tttcactttg agcacgagcc
    25681 ataacaacgt cttgagcaaa tgttccatct tcattcaatg ggctgtttgc ttgggctacg
    25741 atgtaatggt cttcgatatc agctgtcaag tagtcgattt ggtcagtaac acgacctgtt
    25801 tcacggtcta cacgacgata aggtgtttcg ataaatccga atttatttac tttcgcataa
    25861 ctggacaaac tattgatcaa cccgatgttt gggccttcag gagtttcgat cggacacata
    25921 cggccatagt gggagtagtg aacgtcacga acttcataac cggcgcggtc acgagtcaat
    25981 ccgccaggtc ctaaggctga aagacgacgt ttgtgtgtca attcgccaag agggtttgtt
    26041 tgatccatga actgtgacaa ctgagaagaa ccaaagaatt ctttgatact tgccacaact
    26101 ggacggatat tgatcaattg ttgaggtgtc aatgtttcag tatcttggat cgacattctt
    26161 tcacgaacca cacgttccat acgtgctagc ccgatacgga attggttttg taaaagttca
    26221 ccgactgaac ggatacgacg atttcctaag tggtcgatat catcgacatt gccgatacct
    26281 tccatcaagt tcaagaaata gctcattgaa gcaatgatgt cggctggacg aaccgttttt
    26341 acagctgctt ctggatagcc gtttccgatc acgtttactt cacgttcagg gtctcttggt
    26401 gaaaatactt tgatgacttg gacagtcatt ggatctgtga ccacaccgtc ttctgatgga
    26461 tagtaagtca ctgaatttag gccattatca atgaaaggag ctagtgtttc catgacttgg
    26521 tgagtcagaa ctgttccttt ttcaacgatg atctcgcctg tttctggatc aactaatgtt
    26581 tcagctaatg tcagattcaa tagacgtgtt ttcaaatcta attttttgtt tactttgtaa
    26641 cgaccaacgt ttgctaagtc gtaacgtttt ggatcgaaga aacgagcatt cagcaagtta
    26701 cgtgagctgt ctgctgtttt tggttcgcct ggacgcaaac gttcgtaaac gtctttcaaa
    26761 ccttcttctg ttcttgagtc gcttgcgttt ttatgcaagt ctttttcgac tgtattgcgt
    26821 aatgtttcgc tgtcgccgaa gatttcaaag atcgtatcat cggaaccaaa tcctaacgca
    26881 cgaactaata cggttaaagg aatcttacgt gtacggtcga tacgtacata agagatgtct
    26941 tttgcatcag tttccatttc tagccaagct ccacggttag ggataacagt agaaccgaat
    27001 ccttcttttc cgtttttgtc gacttttcca tggaaataga cacctggtga acggactaac
    27061 tgagaaacga ttactcgctc tgccccgttg atgatgaatg ttccttgttc tgtcattaat
    27121 gggaaatcac cgaagaatac ttcttgtgct ttgatttcgc cagtctcgcg gtttgtcaaa
    27181 cgcaatgtta catgtaatgg tgcagagtag tttgcatcat gcgcacgagc ttctgctact
    27241 gtatactttg gttctttcaa ttcataatct acaaattcca aagatagatt gccattgaag
    27301 tcatcgattg gcaaaatgtc ttcaaacatt tctctaagtc cctcatctaa gaaccattgg
    27361 taagagtctg tttgaatttc gatcaaattt ggtaattcca acacttcact gattcttgcg
    27421 aaacttctac gttcgcgatg ttttccgtat tttactacgt gtccagccaa gctcttcacc
    27481 cctctaaaat gtcattcaaa aagactaccc gatcaattgt tacaataata tttcaaatat
    27541 tatttttagg taacaacaga cattttttgc ggcttttagg caaaaaaaaa acaaaaatag
    27601 aacattctct tgaaacctta cttcgtgagc ttctactttt gtttccttat attaaagccg
    27661 aaacggacaa tatttcgttt ctgccttttc ttcatcagat agtttttgca acttcatata
    27721 ctacacttat tttctataaa agtcaacact tcctggcgtt tcttctgctt tttcgcaaca
    27781 ttttcttaat gttttcaatt ttatgttttc aattatctga caattcttaa ttgacagacc
    27841 tttttatctc ggttatactt tagaaaattc attttgtgga ggaacttcta tgcgaaccaa
    27901 catcaaccaa atcgcaccaa cacaactgaa taacttttac tttagctact ttagataggt
    27961 ttgtattcat catgatggat acaaacgtta aacgacagtt tgtatctatg atggctaaag
    28021 aactttgtat tgcagcaaaa agccacgtag acaaaatgct aagtggctga gcagtgaaaa
    28081 tacaaagtaa aagtatcaag cattctactt tttcaattta ttttccgact tttcaatcca
    28141 tttggacatt ttgtctgaat ggattttttg tttcccaaaa ttaaggagga aaaagaaatg
    28201 aaaaaaaata taatcatcgg attaggcgct gccctgctac tagctttagg aggttgcgca
    28261 caaaaagaca attccgcaga ggcatcagat ggacaaaaaa caattggcgt attgcaactt
    28321 gtacaacata actcgttaga tgctgcctat aaaggattca aagaaggatt agcagaagaa
    28381 gggtataaag aaggcgataa tctaacaatc gattatcaaa atgcccaaaa caatcaagac
    28441 aacctgaaaa gtatgagtga gaaactcgtc aaagaagatc ctgatttgct acttggaatc
    28501 gccactccag cagcacaatc tttattaaat gaaacaacca ctatcccgat gactgtcaca
    28561 gcggtcacag acttggaaca agcccattta gtcacatcca atgaaaaacc tggcggcaat
    28621 gtgactggca cgacagatat ggtgccaatc gaaaaacaaa tcggcttact gttaaatatc
    28681 gtgccagacg ccaaaacaat cggcatcatg tataacaacg gtgaagcaaa ctctaagatc
    28741 caagctgatt tggcagaaaa agaattgaaa aaagcgggcg tcaaagtcaa agtactgaca
    28801 gcaaattcca caaatgatgt ccaacaagtg acgaaaagtc tagctaaaga tgtagatgga
    28861 atctatattc ctactgataa cacgttcgca agtgcggcaa gtgttgtcgg tgaagtcgca
    28921 aaagaaacaa aaacacctgt cgttgctggc gcagttgatc aagtcaaaga cggcggtcta
    28981 gcaacagtag gaatcgacta cgaaagcttg ggcaaacaaa cagggatcat ggcagcaaaa
    29041 atcttagacg gcaagaatcc aagtgaactt cctgtcgaaa cagcgaatga tttggaactg
    29101 tatgtcaata aagacatggc caaagcatta ggcatcaatc cagacagcat caaagaagct
    29161 gaataaaaca caggaggatt ctcatgacaa atttgctctc tcttttctta tcatctgcat
    29221 cgcaaggggt cttatgggcc ctacttgcga ttggtgtata cgtcactttc cgtatcttgg
    29281 acattgccga cttgactgct gaaggaagct ttcctttagg tgcagggatc gcggcagtaa
    29341 gtattacaaa tggctatcat ccattgacag cttgtcttct aggcttttta gggggtgcgg
    29401 cagcagggct tgtttccgga ctgcttcaca caaaattgaa aattcccgcc cttcttgcag
    29461 ggatcatcac catgactggt ttatattctg tcacatcccg tgtgatgggt gcgccgaaca
    29521 tctctttatt gggtcagcca acgatctttc attgggtcga gtcattcggc ataacaaaag
    29581 acaacgctgt actgatcgtc ggtctgcttt ttgctttgat cgttgtatgt ttacttgttt
    29641 tgttcttacg tacagaaaca ggacttgcta ttcgtgcaac tggcgataat ctggcaatga
    29701 gcgaagcaaa cgggatcaac acagataata tgaagatcat cggttatatg atttctaatg
    29761 gatgcattgc tctttctggc tccttacttg cgcaaaacaa cggctttgct gatttaaatt
    29821 ctggtattgg aacgatcgta attggacttg cttctattat catcgcagaa gttttgttac
    29881 gtaaccagcc actatttctc cgtctcttga ctgtcgtttt aggtgcggtc atttatcgtt
    29941 ttattttagc gttagtcttc gaactgaatg ttgaaccaag tgattcgaaa cttgcttctg
    30001 ctttcgtgct agtgatctgt ctttctttcc cgcagatcca gcaaaaattc catttacctc
    30061 aaaaaaagaa aggcggtagt gtcaaatgaa tgacgtctta acattggaaa atctccatca
    30121 tacctttgaa aaaggaacga tcaacgaaaa tcatgtcctg aaaggcatta atctttcctt
    30181 agatcccggc gacttcgtga cgatcatcgg tggaaatggg gctggtaaat ctactttgct
    30241 gaacagcatc gccggtacat tttctgtcga tcagggtcaa attttattga atggaaaaaa
    30301 tatcacgaaa aaatctgttg tcgaacgttc gaaatccatc agtcgcgtat tccaagatcc
    30361 taaactaggg acagccgttc gtttaacggt agaagaaaat cttgctcttg ctatgaaacg
    30421 aggaaaaaaa cgcggcttct tccgtggtgt caaaccacaa gaccgaacat ttttcaaaga
    30481 ccagctggtt cggctaaatt tggggttgga aaatcgttta tcctcagaaa tcggtttgct
    30541 gtctggagga caacgtcaag ctattacgtt attgatggcc acattacagc gtccagaact
    30601 gatcttatta gatgaacaca ctgcagcgct tgaccctgct acttccaaaa cggtgatgga
    30661 attgacagaa aagctgatca ctgagcagaa tctcactgct tttatggtca ctcatgacat
    30721 ggaagacgcg atccgctatg ggagtcgact gatcatgctg catcaaggaa ggatcgtcgt
    30781 agatatccag caagaggaaa aacagcagct cacagtgccc caattattag cgttgttcaa
    30841 acaaaacagc ggcacggcat taaaagacga tcaagtactt ttatcttcat aatcagaaag
    30901 aggatgggac aaaaaatgaa cgcccaccct cttctttttt ctctttcctt ctttcgtttt
    30961 tgctttatgt atcaaccgtt gtttcttcga aaactatttt gttctctcct tcttcctgat
    31021 agaatggatg ggaaaggagc ttgatgccat gacaacgaaa gacaaggtac ttcaattatt
    31081 gaaaaagaca ccagattttc tttccggaga aaagttggca cagcaactgg aagtttcccg
    31141 gacaagtatt tggaaagcca tcaaagaatt ggaaaaggca ggctatcgtt ttgagcacgg
    31201 gcctacaggg tatcgttacc tgccctctga cgtactagat gcaaaagaaa tccagcaaga
    31261 gctacggtcg tttattcctg atttgaaagt acaaacgatg ttgacttctg aatctacgat
    31321 gaaagatgcc aaactggcag cagctcaagg agattctgtt tcgaagttat ttgttgctga
    31381 tacacaagaa caagctcgtg gacgttttgg acggcctttt ttcgctgaac ctggacgagg
    31441 aatctacatg agtctcttgc tccatccaga caaacggttt gatgaattgc ctcaatatac
    31501 gatcatcgca gccagtgcct gtgtgaaagc cattgaatct gtaaccggtc gttctgcttc
    31561 gatcaaatgg gtcaatgata tctacatgga tggaaaaaaa gtctgtggga ttttatctga
    31621 agcagtcagt gatatggaaa gcggcaggat cagtcatatc atcattggga tcgggcttaa
    31681 tttttctgtt cctcaaaatc attttccagc tgatctccaa gaaaaagcca tctctgtctt
    31741 tcccgacgga caagctagtg tcacaaggaa tcagctaatc agcaaaatct ggcaagaatt
    31801 gttcacttat ctttctcacc cagaaaaagc gttggacttc tataaagaaa aatcattcgt
    31861 attgggaaaa accgtgtcgt tcaatcaaaa aggaacgact tatgtcggtc ttgcaaaaga
    31921 aatcggtgat catggcgaac tgatcgtcca aacaaatgaa cagaagatga gtttgtcttc
    31981 tggcgaaatc agtctcacat ctatccgtac gcaataatta tcagatttca caaaaggaca
    32041 cctgatctca tacagtgtcc ttttttctga attctcttca aaagactctc cttattctat
    32101 cagctgacag tttctctatc ctctagaaaa aggtattcta aaaatatcta taaagcatgg
    32161 tacactttct ttaaatgaat caatgcaaaa aacaagagag tcatcagttt acacgatcaa
    32221 aagagatagc aggccgcttc ctttttcaat cttatagata gaccatgggt aacgataaaa
    32281 acaaaaaaga acttgcgact tattttgccg taagttcttt tttagctagt gccttaatct
    32341 cttaatccca acaaacgtct cgcatagatc acctgaccag agtgcatagc agcatcatca
    32401 ataatagaaa cgagacgcat tcctcgtgtg actggtggtg tccagttctc gtcaattact
    32461 tcatctagct cggaaggttc cagccttctt aagtaagaaa tagcaaaatc agcagctcct
    32521 cgcaagtatc ctagcagatc ttctatttga ttagcttgga tctgctttgc ttgttccaga
    32581 ctatgcttcc aatcttgttc gttatctgct atcgtcatcg gaaaccggct ttcccagtct
    32641 tgactatgcc aaattggaga agttcctgct aaatcagcaa tctggaaatc taattctcga
    32701 gcggtatgcc aagccagcca agtcattgat ttgatctgcg gggctctatc ttccactgga
    32761 aaacgattgg cctcctcaat agagacgcct tcaaacaaac gttcaaaccg atcgtatgcc
    32821 cgatccacac tctcgcatag catatctaaa taatccattt attttcctcc tttttattta
    32881 tcctacttac tttttcctaa ttctaggata cctacttata tgttactaca cacccttatt
    32941 ttttacactt taacagtcac catcatcaat agtttggaga ttgaaagaaa aagatagtgt
    33001 cgaactatca attgaacaag aagcaacaat atgaacgttt cattaatatt ctttaaataa
    33061 acaatgattt ttcaaacgaa cagtttaaga ctaggagaaa aagagtataa taggaaaatg
    33121 tttgaaagga aaggaagggg aaaaatgaaa aaaagggcaa gtttactagt cctagttgct
    33181 ttgttgttca atatcttttc gtatggtgtg gccgtttcag cggaaacata cattcctaaa
    33241 gaccctggga atatttataa tgatctaact ggtaactatg gcttgttagg aattgctagt
    33301 caattccacg tatttgctaa aaataagaca actgtgaatg cacatacaga cggaaatata
    33361 gcaacacatg aacttgatgc aaaaaataat tttggtacag agcttcatga aggtttattg
    33421 aaccaagaaa taaattatat acaaaaaact gattctttcg ctaattcttc aggcatacct
    33481 acaggtagca caagaatgaa taaatttgtc gttggcagta acgtggatgt agcacttcaa
    33541 gatggtcatc ctacagtcaa tggatccagg cttgatcact taactccaga tgatttttat
    33601 aaagatcgtg ctgggcatga atatattgat ttcgagcagg aatttgctaa attgaatgta
    33661 gcagcaaatg atcttgcaac gatcactcca gctaaaacat atactgctgc agattttcca
    33721 gacatgaaca atcggacaat cgatttaact gatttatcag ataccgggtt tttattggta
    33781 aatatcgatg cagaagtgtt aacgatgaat acgccacttc aaatcattaa tccaaatgat
    33841 caagtagttg tatttaatgt catcaacagt tcttctgcat tgaacgtaca gtcaccaatc
    33901 aaatacaatg atcgttcaaa tcatgaaaca gaagattttt cggatgcgaa tatctcttgg
    33961 aattttggaa atgagatgac tgatttgaca atcagtgctc cttttcaagg aacaatcctt
    34021 gcgcctaatg ctactatccg cattactcaa aaccaagacg gaacgattat aggaacaaat
    34081 gtgattttag atgcagctac caatcgttgg gatcctaacg aaatatttat cagcgacaat
    34141 ggtacagata cgacagacac aagcgacagt acagatacat ctaccacaag tgatagctca
    34201 gattcatcaa cttcaagcga cagtacagat actacctcta ccacaagtga tagcacggat
    34261 acatcagctt caagcgacag tacagatact acctctacca caagtgatag cacggacaca
    34321 tcagcttcaa gtgacagtac agatactacc tctaccacaa gtgacagcac ggacacgtca
    34381 gcttcaagcg acagtacaga tactacctct accacaagtg atagcacgga cacatcagct
    34441 tcaagtgaca gtacagatac tacctctacc acaagtgata gcacggacac gtcagcttca
    34501 agcgacagta cggatacatc taccacaaca agtgatagca cggacacgtc agcttcaagt
    34561 gacagtacag atactacctc taccacaagt gatagctcag attcatctac cactacaagt
    34621 gatagcacgg acacgtcagc ttcaagtgac agtacagata catcctctac cacaagtgat
    34681 agctcagatt catcaacttc aagcgatagt acagacacgt cgtctaccac aagcgataca
    34741 cgtcatgcaa gtgtctcctt ctcaaaaaca agttcggata acaggacgaa ctttaactta
    34801 actaataaaa caagcaatga caatggaaac aaatcaaaaa gagcacttcc taaaactgga
    34861 agtcaatcga ataattggat aacactcgca ggcgttatat tacttgtaat cggtttaaga
    34921 atcactttta tgagttataa gaaatcgaaa aggtaaaaaa atattcaaga taaaaaagag
    34981 caacgtcgct aacggacgtt gctctttatt tttgcttttt atcataaata caagaactac
    35041 aattcaattt gtcaaaaaac acgtagattt ccttctgaga aatttattta cgtatcgaac
    35101 ctttccctat tcagtctttg cgatccaaaa tcagttgggt aagggaaaag agattctctt
    35161 ttgttcgaag agaggtggca ggtaatttct ttatttcctt caaagccttt tctgtgtaag
    35221 aggccgctaa cttcctagct tcttccacgc ctcctgtatg ttcaacgatt ttgcgtacgg
    35281 cgtcgacatc ttcttctgtc attgccgctt tcttctctaa ataaggtagt agctttggtt
    35341 tgtttttttg caaagagtag atcaacggta aagaatagac cccttgacgc atgtcttcta
    35401 gtacaggttt gccgatttcc tggctctctt ttgtataatc taaaatatca tcgataatct
    35461 gaaaggccat cccgatagaa agtccgatat ctcctgcttt ttttgcgaag cgctcgcttg
    35521 ttccactttc aaaagcgcca acagaacaac ttaattggaa cagttcagct gtcttacctg
    35581 aaatattttc caaatattct tttacagaaa gttcataatt atagcgatca tccatctgcc
    35641 ctaattcgcc attcaatact ttttccatac tgcgagaatt caattggaga cttttcaagg
    35701 aagtggagta atcagacaat agtttaaaac agcagacaaa taaatagtct cctgcataga
    35761 cagaaacgac attaccaaac tgactacgga tattaggctg accacgtcgc agatccgctt
    35821 catcaacgat atcatcatgg atcagtgtgg ccgtatggag catttcgata gaagctgcca
    35881 aagcgatggc tttgtctcga tcttgctctg gtccaaattg tgagaacaat agctgatagg
    35941 caggacgcaa cagctttccg ccggcatgga tcgtttcttt gatggcttgt tcgacaggtt
    36001 tgtttttcag gcggacagtt tcttccatcc gttttagtgt ttctgtcaat tctttactta
    36061 aagcaggata gttttcccac attggatgta gcttcataaa aaaactcctt gtgtgacgat
    36121 tattttttgg taaacattgg gctccatttt tgtttggttc cctgctcata atctttttgg
    36181 aatcgttgaa gtgtctgcac atgtcgtaaa agataattcg ctgcgatccc gattgcaatc
    36241 cctgataaaa tccctaaaac tgaaaggact ggcaagtaca acatgactgt ccatgattgt
    36301 gcaatgaaag aagccatcaa aagctgtccg acattatgca tgaaaccgcc ggccgcacta
    36361 attccgatga tactgacacg ttttggtcct aattgcttga ccaataacat tccaaaataa
    36421 ctaagcaaag caccactcat actataaagg aatgtggaaa tcgttccgcc tagcaaagta
    36481 gtcaggaata gtcgtagcca aagtagtaag aagctgtctt gttttttcat ggtaaaaatg
    36541 gcaacgatcg tgattagatt ggctaagcct agttttgcac caggcgcaaa agaaaaagga
    36601 taagggatca tattttccaa taaaccaatg atcactcctt gtgcaacaag tagcgaaata
    36661 taaatcattt tttgtaattt actcatggtg tgtgaaggtc taataaatca gactgtcttc
    36721 gtcacctcca tctgttgaac ggacttcgat cactaattta tgtggcaaac agacgatggt
    36781 ttcgccattt ttcgttgccc agcctcgacg tacgcagatc tggtcatcgc aatctgcttc
    36841 ttttatccgt actcgatcgc catctatctc gatcaagtta taatctccgt gttcatcttt
    36901 gtaagtatat gtgttgctct cttctccagc catcaaaggg aatgttttga tttctgtgcc
    36961 atccacacgc agtaccgctt ctttttctgc tgttgcggtg ttttgctgaa gaccaaaaat
    37021 catgactggt aaaaaagaaa gaacagtcaa aagcaaaata ataaaaacat cccaaggttt
    37081 gagcagactt ttttcagcaa attctttaag gctcattttt gttctccttt ttccagatta
    37141 actcacccaa ttttagcata aaaacaagag gttgtgacac ctgtgtcttg ctttgagtaa
    37201 taagaacgaa gaatagaaaa cagctttcca tgttttctaa ttctttgggc ttattaccga
    37261 gaagcagcag gtggaacacc gtttataaca agaggttgtg acagaagtgg gcaacttcaa
    37321 gaaataagaa ggaatttacg aaaattactt ttcaagtttt tgtgaaattt cggcttattt
    37381 ttcgaagaag ctacttctgg aacaccgttt ataacaagag gttgtaacag aggttgtgac
    37441 acctgtgtct tgctcatgga ggcttatctc gggcatctta ttcctttaat tttacataaa
    37501 aaggaggctg tgacggtttg tctcagcccc tttctattga aatcttgcat tcaaataacg
    37561 ttctttatct atttatttct tgccgtactg ggaacttggt ctaccgtacc accaattacc
    37621 tagtgtacgt tgtagccatg gaattaccca gcgatccaac ccaatcgcac gtccggaacc
    37681 gttcatcaat gcgaaagcaa caaaaatgaa ccaaatgttt acccagtaaa acatgcctga
    37741 taagcagaac gtgatcacca atacaatcgt tgtcgcactg catagccatg tgaataatcc
    37801 agcaatcaat gctaatgcca aggcaatttc aaagaatacc atgaattttt ggaagaacat
    37861 tgctacatca ttgtttggca tgatcacttg catgatcttt gtgaaccagt ctggtgactt
    37921 ctcaatcaca gccattggtt cttcaccgta tgcataactc aaaccaaaat gtgcagcttg
    37981 agtcgctgct tctccggcac ttgcccctgc tccgctggca gctgtggttg catcaccagc
    38041 agcagaagca ccactggcaa catctgttac attgtattcc catgtccaag ggaacacact
    38101 gtttccagtg aaccatgatc cttcaccaaa ccaagtactt ggtttgaaca cttcaccatt
    38161 cccaatgatt tttttggaag cttcaacgaa ccaaactaaa ccatagaaaa tacgaagtgg
    38221 cacactccat aagacattgc cgtaacgaga agaatgtcct cgagtcacgt tgcgatcatc
    38281 tttgatatgg aaaaactcat gcattaaata ttggaacata taataaccag aacgaatatc
    38341 aaagaagtat ttcaaattca cgatatgttt cattacgata gctaaaaatc cgcttaaatg
    38401 gatcttatca aataggttag ctacccccca tttggcacca atagaaacca taaatccttg
    38461 atagtttcct ttaaatttat gcttttctcc gcctttgatc gaagccacga tattcgctgc
    38521 ggctgtgtgt cctgtttgtt cagcagcttg aacgatctgc ggtgtcggtg ttcctgggaa
    38581 ttcttcataa aagactaaat caccaatgat atagatgttt ttatcttcgt agccttttgc
    38641 ctgcatatat tcattggcga tcaaccgctg tccgcgtgct gattctaatc caaagtcagc
    38701 agcgtcagaa gttcctttga caccagctgt ccaaatcagt gtgtgagtcg gaatgttgcg
    38761 tccgtctttc aatttgatat gatcagcagc cacttctacg attggtgcat tcaataataa
    38821 ttccacgttg tgcttacgca aatagcgttc agcttttgca gcatcatttc gatccaacat
    38881 attcaagatc gtcggcattg cctctacgac catcagtgtg atttcgtttg gatctaattt
    38941 ataatctttg gctaggcgat ttttccagtc gatcagttcc ccaatcattt caataccggt
    39001 aaatccagaa ccacagacaa cgaaagtcag catggcttta cgcagttcat catctggttc
    39061 caatgcagct tttgctactg tcttctcaat atgttcccga atccttacag aatcttcaaa
    39121 tgaccaaagg gtgaatccgt gtgctttgac accaggtgta ccaaagtcat tcggctctcc
    39181 gcccattcca aggatcagct gatcaaaagg ataggaacct aatttggtct tgacgatttt
    39241 tcgttcttta tcgataccga ttacagtgtc tgtgacaagt tgtacattct tttgacgagc
    39301 aaacaaatgc tgcagatcgt attggattgc ttctggttct acacgtccgc cagctacttc
    39361 atgcaactcg gtcatcatag tatggtacga atgacgatcg atcagagtaa ttgctacatc
    39421 ttcttttttc agtttctttg ccaagtattt tgtagctgaa acaccggcat atcctgctcc
    39481 tacaacgaca atattttgct ttgccattga tcatccgctc cttatagact atctatataa
    39541 tgaatatatg ataattcttt tagtttgata cttattacac aatctccata tattatatag
    39601 atgttctgtt cttttgtcta actccttttg gatttttttc aaattttaaa ataaagcgtt
    39661 ttttcacgaa atttgctgaa agaaaacgtt tgaaatatta catgggttat ttatcaaaaa
    39721 tagttgactt ataggctaaa aagattagaa tgtataatga atcacaaaga aaaattgtgg
    39781 actagaaatg gaatggtggg gtattaaaat gaagaaaaaa cgttttgtaa ctggatttgc
    39841 cgtattagca ttttcagctc tagtgttagg cgcttgcggc gcagatgaca acggaagctc
    39901 aaactcttct tctgaatcat ctacagcaca atcttcgact gcaaaaagca caacagaatc
    39961 atctgcaaaa gttgtagctg gcggagattt gcaagacggc acatataaat tagaagaaaa
    40021 aaattattca aatggttacc gtgcagtctt tgaaatggtt gtaaaagacg gcaaaatcac
    40081 tgaatcaaaa tatgataacg tcaatgaaaa cggcgaatca aaaacaaaag atgcagaata
    40141 caacaaaaac atggaagcaa aatctggcac aaacccagaa aaatacattc ctgagttgaa
    40201 tgaccaattc ttaaaagcac aaagcgcaag cggtgtggaa gtcgtaactg gagctactca
    40261 ctcatctgaa agcttccaaa actacgcaca acaattgatc caagctgctc aagctggtaa
    40321 cacagataca atcgaaatcg acaatggtgc tgatttgaaa gacggcactt acaagcttga
    40381 agaaaagaac tactctaacg gctaccgtgt gcaattcgaa atgacagttg ctggcggcaa
    40441 agtaactgaa tcaaactttg attatatcga caaagacggt aaatcaaaac aagatgatac
    40501 agaatataat gaaaatatga aagcaaaaag cggtacagaa ccaaaaactt acatccctac
    40561 tctgaacgac gaattcgtca aagcaatggg cgaagaagat ggctcacctg ctaatgtaga
    40621 agttgttaca ggtgctacac acagctcaca ttcattcatc atgtacgcac aacaattagt
    40681 gaatgcagct gaaaaaggcg acactcaaac aatcgaagta gataatatcg taacagaaaa
    40741 ataatcacat aatcatacta ataaaagttg gagggtggga cgatagccag attaggcagt
    40801 cgtctcacct ttcaaaatga agagaggaaa aagatgcgga aaaaactact attttcctta
    40861 atcacactgg gcatctcttt tatggtgcta actggctgca gcaactcagc aaataaaaca
    40921 accactgaaa gttcagcagc taccaagata ttgaaagatc catattctga tgaacagttc
    40981 ttgttaggga cttacgttcg tatccgtgtg tatgatgaag gcaaagaatc agcattgaaa
    41041 cctgccttcg atcgagtaaa agaacttggt gataaaatca cgatcaatca aaaaggttct
    41101 gagatcgatg aagtgaatga gcaagctggg atcaaaccag taaaagtttc tgatgacgtc
    41161 tacacgttag tcaagcgggc ctacgaatat agccaagatt ctcaaggcgg attcgatatg
    41221 gcgattggtg caatcacaca attatggcga atcggttttg atgacgccag aaaaccttcc
    41281 caagaagaaa tcgatcaggc attgaaatta gtggattatc ataagatcga actaaatgac
    41341 aaagaaaaaa ctgtttatct aaaagaaaaa ggaatgatca tcgatttagg tgcaatcgcc
    41401 aaaggttaca ttacggacga agtagtgaaa gtcttgaaaa aacaaggagt gaccactgcc
    41461 atcgttgact tgggcggtaa cgtctatgtc ttaggccaca gcccaagagg cgaaaaccaa
    41521 gactggacag tcggcatcca agatccgaac atggctcgcg gatctgtttt aggttctatt
    41581 aaagaacgga acaaaacatt agttacctct ggaatttacg agcgttattt agaagtagat
    41641 ggcaaaaaat accatcattt gtttgatcca aaaacgggtt atccgttcga caatgatatc
    41701 gcaagtgtca cgatcataac agataaatca attgatggtg acgggttgtc tacagcagtc
    41761 ttttcaatgg gtgtgaaaaa aggactggaa tacgtcgaaa gcgaattgaa taacgggacg
    41821 gaagcgatct tcgtgacaaa agacgataaa gtatatgtga ccgatccaat caaagacacc
    41881 ttcaaattat cggaggactc ccattacacg atgggtgacc gttctgaact aaaataagga
    41941 gtacgaacca tgacactaaa aatcttttta gaagtagttg aaatacgaac aaaggtggct
    42001 agtgtatttc cttttgcggt aggggtatta ttctctatcg ctttttttca tgagatacat
    42061 tggctaaaca cttttttatt ttttattgga atgctagttt ttgatttagc gactaccgct
    42121 atcaataatt acatggatta tgaaaaggct cattctgaag tatataagta tgaagaaaat
    42181 gtcattggcc gcagaaatct atctcctgta ttgattcgaa acatgatttt agctatgatt
    42241 gcatttgttt ttgttatcgg cgttattcta acgatcaata cgggttggct tttacttttg
    42301 atgggattta tctgttgttt tattggagta ttctatacat tcggaccgat tcctttatct
    42361 cgaatgcctt taggggaaat tttcagtggg gtgactatgg gccttggaat ttttgccatg
    42421 gtcgtttatg tcaatacatt cggttcagat gtctatgatc tgacagtcaa cttttcatct
    42481 ggacaatttg atttggtagg aaatctttgg gctatagcag caatcgtatt agcttcctta
    42541 cccttggtgt ttacgattgc caatatcatg ttggctaata atttgcggga tcttgaccgt
    42601 gatattgaaa atcatcgcta tacactagtt ttttatatcg gacgtcctgc gggaacagtc
    42661 ttattccagc tattgatgta tgcttgctat ttcgtgattt taatggggtt gttgagcggt
    42721 atctatgaat ggccgatctt ggtcaccttc ttgaccttgc caattgtgaa gaagcatttg
    42781 gatcaattca agcaaacgtt accccagcct atcagtttca gctattccat caaaaatatg
    42841 gtcttattca atgccagtta tgtattgggg ctattactaa caatcattat gcaagccatt
    42901 taacagcatg aagaggttgt gaaaaaactg ccctgcatca agtaataaga acagccaaat
    42961 aaaaatagct ccttatattt ttcgtttggt tgggcttatt accgcagatg caagcttttg
    43021 aacaccgttt atca
//
LOCUS       DAZUMK010000012        41595 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000012 DAZUMK010000000
VERSION     DAZUMK010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.012"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..677)
                     /locus_tag="ACG808_000637"
     CDS             complement(<1..677)
                     /locus_tag="ACG808_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5046290.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKIC"
     gene            825..2060
                     /locus_tag="ACG808_000638"
     CDS             825..2060
                     /locus_tag="ACG808_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFS5046291.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKPEVAPEEKPDVTP
                     EPDTDSGNQTVPETNPDTDNETENPEKPEVDPEEKPDVTPEPDTDARDQGIPEKINKK
                     TIQEDGKKESKKSNLAILKINEEQLNKKSRIFDSAQSAETLKSSKDTTFAVLKRKINS
                     YQNPESHKTKLYCGAESFY"
     gene            2033..2113
                     /locus_tag="ACG808_000639"
     CDS             2033..2113
                     /locus_tag="ACG808_000639"
                     /inference="COORDINATES: protein motif:HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046292.1"
                     /translation="MLWSGIILLSIATMLSAKRFKQNRSL"
     gene            complement(2239..3318)
                     /locus_tag="ACG808_000640"
     CDS             complement(2239..3318)
                     /locus_tag="ACG808_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS5046293.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(3421..3861)
                     /locus_tag="ACG808_000641"
     CDS             complement(3421..3861)
                     /locus_tag="ACG808_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS5046294.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3918..4328)
                     /locus_tag="ACG808_000642"
     CDS             complement(3918..4328)
                     /locus_tag="ACG808_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFS5046295.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(4508..4942)
                     /locus_tag="ACG808_000643"
     CDS             complement(4508..4942)
                     /locus_tag="ACG808_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5046296.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(5042..5890)
                     /locus_tag="ACG808_000644"
     CDS             complement(5042..5890)
                     /locus_tag="ACG808_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS5046297.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            6279..8045
                     /locus_tag="ACG808_000645"
     CDS             6279..8045
                     /locus_tag="ACG808_000645"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFS5046298.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            8147..9406
                     /locus_tag="ACG808_000646"
     CDS             8147..9406
                     /locus_tag="ACG808_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS5046299.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            9553..10854
                     /locus_tag="ACG808_000647"
     CDS             9553..10854
                     /locus_tag="ACG808_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5046300.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            10857..11708
                     /locus_tag="ACG808_000648"
     CDS             10857..11708
                     /locus_tag="ACG808_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFS5046301.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            11721..12536
                     /locus_tag="ACG808_000649"
     CDS             11721..12536
                     /locus_tag="ACG808_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFS5046302.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            12687..13853
                     /locus_tag="ACG808_000650"
     CDS             12687..13853
                     /locus_tag="ACG808_000650"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5046303.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            14083..14730
                     /locus_tag="ACG808_000651"
     CDS             14083..14730
                     /locus_tag="ACG808_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFS5046304.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            14814..15758
                     /locus_tag="ACG808_000652"
     CDS             14814..15758
                     /locus_tag="ACG808_000652"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFS5046305.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGTEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            15893..17344
                     /locus_tag="ACG808_000653"
     CDS             15893..17344
                     /locus_tag="ACG808_000653"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFS5046306.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            17365..18201
                     /locus_tag="ACG808_000654"
     CDS             17365..18201
                     /locus_tag="ACG808_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFS5046307.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            18201..18860
                     /locus_tag="ACG808_000655"
     CDS             18201..18860
                     /locus_tag="ACG808_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS5046308.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            18986..19675
                     /locus_tag="ACG808_000656"
     CDS             18986..19675
                     /locus_tag="ACG808_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046309.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            19681..20637
                     /locus_tag="ACG808_000657"
     CDS             19681..20637
                     /locus_tag="ACG808_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFS5046310.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            20681..21193
                     /locus_tag="ACG808_000658"
     CDS             20681..21193
                     /locus_tag="ACG808_000658"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5046311.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(21449..21997)
                     /locus_tag="ACG808_000659"
     CDS             complement(21449..21997)
                     /locus_tag="ACG808_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFS5046312.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(22086..23266)
                     /locus_tag="ACG808_000660"
                     /pseudo
     CDS             complement(22086..23266)
                     /locus_tag="ACG808_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
     gene            complement(23502..25010)
                     /locus_tag="ACG808_000661"
     CDS             complement(23502..25010)
                     /locus_tag="ACG808_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFS5046313.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            25169..25360
                     /locus_tag="ACG808_000662"
     CDS             25169..25360
                     /locus_tag="ACG808_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046314.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            25563..26480
                     /locus_tag="ACG808_000663"
     CDS             25563..26480
                     /locus_tag="ACG808_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFS5046315.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            26473..27546
                     /locus_tag="ACG808_000664"
     CDS             26473..27546
                     /locus_tag="ACG808_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046316.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            27694..28212
                     /locus_tag="ACG808_000665"
     CDS             27694..28212
                     /locus_tag="ACG808_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046317.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            28193..28585
                     /locus_tag="ACG808_000666"
     CDS             28193..28585
                     /locus_tag="ACG808_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFS5046318.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            28661..29596
                     /locus_tag="ACG808_000667"
     CDS             28661..29596
                     /locus_tag="ACG808_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFS5046319.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            29729..30508
                     /locus_tag="ACG808_000668"
     CDS             29729..30508
                     /locus_tag="ACG808_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFS5046320.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            30501..30818
                     /locus_tag="ACG808_000669"
     CDS             30501..30818
                     /locus_tag="ACG808_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFS5046321.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            31062..32672
                     /locus_tag="ACG808_000670"
     CDS             31062..32672
                     /locus_tag="ACG808_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFS5046322.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            32829..33677
                     /locus_tag="ACG808_000671"
     CDS             32829..33677
                     /locus_tag="ACG808_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS5046323.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            33908..34387
                     /locus_tag="ACG808_000672"
     CDS             33908..34387
                     /locus_tag="ACG808_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFS5046324.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            34420..36648
                     /locus_tag="ACG808_000673"
     CDS             34420..36648
                     /locus_tag="ACG808_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5046325.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            36777..38420
                     /locus_tag="ACG808_000674"
     CDS             36777..38420
                     /locus_tag="ACG808_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFS5046326.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(38493..39038)
                     /locus_tag="ACG808_000675"
     CDS             complement(38493..39038)
                     /locus_tag="ACG808_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFS5046327.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(39061..39546)
                     /locus_tag="ACG808_000676"
     CDS             complement(39061..39546)
                     /locus_tag="ACG808_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS5046328.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(39536..40348)
                     /locus_tag="ACG808_000677"
     CDS             complement(39536..40348)
                     /locus_tag="ACG808_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFS5046329.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(40487..>41595)
                     /locus_tag="ACG808_000678"
     CDS             complement(40487..>41595)
                     /locus_tag="ACG808_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFS5046330.1"
                     /translation="ILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAHS
                     MGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKVV
                     HMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTSM
                     AQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLPE
                     NTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLIE
                     TINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELPR
                     LNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        13843 a         7523 c         8100 g        12129 t
ORIGIN      
        1 ttacatattt ttaatagaaa atataattta tctatcatac cgctatttag tcggtcaagt
       61 acttcttttt taaaaaaagt attcatcata aaagccacta cttgcggatc ttcatcataa
      121 aattctgtag cagtacttga aaagatgttc caataaatat ctaatgctgc atattgaatt
      181 tgtacctctt ttccagaaaa agactttctg ccattattag aaaaggagat agacaaaccg
      241 tacaatgcta aaaatgcatt aatctttttc attttcgtat acaaataaga ggcactatag
      301 tttgtttgct gacttaaact gataatcgaa tttgtctgac agctgaacaa agaagtcaag
      361 atctgataac tgactgattc gttgcagtat cttccaaata gacgataata gcagtaagcc
      421 ggatcaattc cttcaaaatt caactttaaa aaaggttttt gcttatcaat atgcagttct
      481 gttagattga gacgctcaaa gtcttgttct aattctttga tgtatagtaa aaaatttgat
      541 tcagacatat ggaaatgatc tgctaaatat tgtgtagtaa taaagaaacg atgttcagat
      601 aggaaaagaa aaatatctaa ttttgtattg ctttttttct tcaagagaaa cgttttaatc
      661 gcttcttgag tttgcatgaa agaggctcct ttttacttaa attgttaaaa cttgaaaata
      721 ttactgtttt tttcataaat ttagacaaaa aaacagtaaa catataggta gaatctctac
      781 tatcttattt cttgcataca aaaaaatcaa ggagtgagag agttatgaga tattcaacca
      841 tcttaatatc aacatttgca gccttaagct tatcgcttac tataaatcag acagtccaca
      901 cagaggagat agaaacagcc aaatggacag aaaccaagtg ggccaacgtc aatcgaatta
      961 cctttcagga tgaattttca tccttcaatt caggagtcgc aaaattaaga aaaggacaaa
     1021 cagcgaccat caaagcaaaa attgactatt caggagactt agcacctata ctacaatcag
     1081 ctgttacatt tgagaatgtt gatcccgctt tgtcgattgg ctatgacgag aacactttga
     1141 ctatccaaca caagcaagcg atatttacct tcaccgttac attaaatcag gacttgttaa
     1201 aaccagcgct ttttactgtc aaggtatcag atggactacc gaacgatcat catcatttaa
     1261 cgccgtatag tcagcggcag acaattgagc aagattcagc tatcgactca ggaggagatc
     1321 ttgttgaaga accaacagat aaaccagaaa atgaaaataa gccagaagta ccgcctacag
     1381 aaaatccaga cggagaacag aaaccggaaa tagaacctgg agaagaaccc gatacagaaa
     1441 ctcaaccaga gccagacaat gaatcaaaac ctgaaataac ccctggagaa aaaccagatg
     1501 tagacccaga agaaaaacca gatgtgactc cagaaccaga tacggattct ggaaaccaaa
     1561 cagtaccgga aacaaatcca gacacagata atgaaactga aaatccagaa aaaccagagg
     1621 tagccccaga agaaaaacca gatgtaactc cagaaccaga tacggattct ggaaaccaaa
     1681 cagtaccgga aacaaatcca gacacagata atgaaactga aaatccagaa aaaccagagg
     1741 tagatccaga agaaaaacca gatgtgactc cagaaccaga tacggacgca agagaccaag
     1801 gaataccaga aaaaataaac aaaaaaacaa ttcaagagga tggaaaaaaa gagagtaaaa
     1861 aatcaaatct agcaattcta aaaataaatg aagagcagct gaataaaaaa tcacgaatat
     1921 ttgatagcgc ccaatcagct gaaaccttaa aaagtagcaa ggatacaacc tttgctgtcc
     1981 tgaaacgaaa aataaacagc taccaaaatc cggagagtca caaaacaaag ttatactgtg
     2041 gagcggaatc attctattaa gtatagcaac aatgctttct gcaaaaagat ttaaacaaaa
     2101 cagatctctc taacatttga ataatgtctg tgaacgcatt tttcaaataa aaagaaatat
     2161 ccagaaaatg accccaaaat accagattga aatctgatat ttggggtcgt tcatacttta
     2221 tctttcttca tatttatttt aagggatatc tgtttctttt ggaagatggt tcgggatctt
     2281 tttcactttc aatacatcta aaaagaaggt aaagatcgga atgccgacaa tcaagcccca
     2341 aacgccaaag aaccgttcgc taacaagtaa gaccacaaat gtataaaaaa tcggcagttc
     2401 tgttttgctg gacatcaact tcggattcag cacataagac tcaattagat ggacaatcaa
     2461 aatcgtaatc aggatataaa caacatctcg gatgcctcct tgggaataag ctatgaaaga
     2521 tagaggaata caagaaatga taacacctgc tactgggatc agacttaaaa tgaaaatcat
     2581 gatcgctaaa ctcagcagct gatgaaagcc aaatacagcc aaggcgattg tagtgagcaa
     2641 cgtatttaaa agagcgacaa caaattgagc ttctaatacc aaaccaaatg tattgacaaa
     2701 cttatcggca aaatagtaaa tatcttgaaa gaaccaagaa aattcacttt ttaaaaacaa
     2761 tcttgagaat acaattgttt ttttcttttc aatcataaaa aagaaactta agatgaatga
     2821 catagcaaac gccactgcca tttttcctat atcctgtaaa tagccaagcg caatcgaagc
     2881 gccattttga agctgttcaa aaaaatcgtt tcgctcaata taagtatgga tatattgtaa
     2941 aacaggatct gcatcttttg gctgatgctc gtaaaaatta ataacggact ggatcatgtc
     3001 aaatgtttga tgaataagca tcgggatata ctttgtgaat gccagataga taaggtaaac
     3061 gatcagtgta taaatcatga tagctaaaac acctgtaggg attcggacaa atcgctgaat
     3121 gatttgaaca agatgaatca tcaaataagt aaagataaat gtgagcaaaa tggttgtaat
     3181 catacttcta actaagaaaa gaacaaaaac gacaaataat aaaacagtga gacggcgtat
     3241 tttttcattc gccaaaaaac gctgataaac ggtcaaatcg acaacctcca aataacaaaa
     3301 aataaacacg ttattcatta tatgatggaa tatgaaaata agaaagagca aaaagaaaaa
     3361 ccgctaaatg acgttatctt atgagttggt tttcctcata aaatcgttca tctagcagtt
     3421 tcagcccttt ttctggtctt gatgaaaatt atctagcatt tttcgggtca gatgttgcag
     3481 tgtatccaat tcttcctgtt caagtggaag ataagacttg atcttttgtg gaataccttc
     3541 tgcttgtttt tgcaacgcag caccttgagc agtcagcgta acatttacga ttctttcatc
     3601 acccgtttca cgctgacgat taatataatt tttttctgct aactttttta gtaagggagt
     3661 cagcgtacca gaatccagac acaaatcatt tcctagctct ttcactgaag tctttttctt
     3721 ttcccataaa actaacatca caagatactg ggtataggta agattcagcg gcttgaggag
     3781 gggagtgtag tgtctaatga tttctttaga ggcggcatat aaaggaaaac atacttgatt
     3841 aactaaatat agatcttcca tttttccacc tccgctgttt tactatagtg tatcatagcg
     3901 gatttacaca aaaaagtcta ttcactaact gctttcagag taacttctat atttccagct
     3961 gtagcttttg aatacggaca cactttatgc gctgcctcta ataattcttg cgtttcttcc
     4021 agagaaagac cttcgataga tccttcaatc tctacaccta agacaaagct cgtttcactt
     4081 tctgaataaa gggaaacttg tgcactgaca gtagaagccc cagagatatt tttttgctgt
     4141 ttgatcaatt ctaatgcact gttaaaacat gcactgaaac cagctgcaaa caattgttca
     4201 gggttggttg cattttcgac gcgtttacca ggagctgcaa tattcaattc gaatgtacga
     4261 tcgggattat gcacttctcc agagcgtccg cctgtgttga tcattttcgt agaatacatt
     4321 tttttcataa aattaccacc tttaattata ttttatacaa ttgaatttta cacaatataa
     4381 ttgtggaaag caaagaaatg gattggcata agataaataa gagaaagcac atgaaacaga
     4441 cgcgctttct cgaatagcaa acatatacaa aaaatatcag agacaaaaaa taatctcatc
     4501 ataaacatta catatatttc tcaaaaagtt gttttctttc ttcaaaaatt acttcacgat
     4561 ggacctgatg aagcatccat aaataaccaa aaggatctct gaacattgag ttagtcacac
     4621 caaagtccat catttctgtc atttcttgaa tctctacaca gccatttcgt aatgctttat
     4681 tatgtgtgtc ctcgatatca gagacgatga tattgaacca gatcgtattt ggatgttcag
     4741 cagtaggagc agtcaaacca agagacgtgt tttcatccag catatggaat ctcgtaccat
     4801 acaaggtaaa aatgacttca ttattgcctc tagggtaatc tgttgcctca atcagttgta
     4861 tttcatcaaa gattgtttga tacagttcta gtgctttcaa actatcattt aatacaaaat
     4921 cgatttccgt tcctttatgc ataaagatct cctctccttt tcagtcgaac agttactaga
     4981 ttcttttatt ataccagcta aagaaaacgc attcaaaaaa atagcggatt tacttaccaa
     5041 ctcattcgat tggatgataa gtaaatccgc gtcccattac ttctgctacg ctgctgacaa
     5101 tgacaaaggc acttggatcg atttcttcta tcaatggttt cattcgggaa aattcatgat
     5161 cagtcacaac gatcaataaa acctcttttg actttcgatt atacccgcct tttgcctcta
     5221 gcagggtaag gccacgtcca atctcagcac ttaagcgaag gcggatgtct tccaaataat
     5281 gttcactcat gaccataaca gatttctttc gattgatacc tgtttcgata taagtcatga
     5341 caattgaagt gattgcaata gataaaatag cataaagaaa ctcttctatt ccaaaaataa
     5401 acagattaag agaaacaacg aatgcgtctg taaaaaataa gccaatcgaa gtattcaagt
     5461 ggaaatattt tttaaaaatg aggggaggga tcgtcgtacc gccgctagaa gagttgtttt
     5521 tatataaaat cgcaactcct aaagcaaaaa tcgcacttcc aaaaatgaca gacaacaacc
     5581 gatctgaagt aatcattgtt tctggaacta ttgcaatgat taaaggaaaa accaagcttc
     5641 cgaaaagtac tttaaaaaag ggttttttac ctaaaaatag aagagctagt accaacataa
     5701 cgatattcaa cactaaaata actatagctc ggtcaaagcc taaagcagct tccagtaata
     5761 ttcctagacc gctgacacct ccagcagcaa tatgatgagg ccctaaaaac atattgatag
     5821 cgactcctaa tatgaagagg gcaaaaataa tcttaatgta tgtccatata aattgtctcg
     5881 tttctttcat ttattcactt ccttaattca ttaataatac tcttatctta ttataactta
     5941 cttttcacct taagttaact ttaattaata aaaaaattag gctagtctaa attattatgt
     6001 ttcattcttc tagaatatac ttctacgcca aaaactgtta aaaaactttt actttagaaa
     6061 attggagaaa ttgatcgctt aagataaatt caggacacta aatagtacag gaagtagact
     6121 aaaactttta aaagttttaa gtctatctat ttggcaagtt taaaaaataa aaattcggta
     6181 tcaaaaaact gttaccgata acatttttga aagcgttgtc ttttgcttct ctatcactta
     6241 tagtgatgac gtcatattaa aacacaagga ggaaatcaat ggatacagct gcaatttatc
     6301 atcgaccgga aagtgaatat gcatttctat atacgaatac aagatttcgt attcggcttc
     6361 gtactaggaa aaatgatatc aaaaaagtat atgtgctatg tggagatcca tataccataa
     6421 caactgaaaa atggtatcaa aaacaacgac caatgaaaaa atgtctaagt accaatgttc
     6481 atgattattg ggaaattgaa gtgacgagcg aaacaagtcg cttacagtat gcgttccatg
     6541 ttgttgggga agataacacg gactgttttt acggtgatca aggaattttt ccttatcgag
     6601 aaaatgtgat tactgaacca aatttttatt ttcgaatacc ttattttcat caaatcgatc
     6661 gattcactat tccagaatgg gtaaaagaaa cagtatggta tcaaattttt cccgaaagat
     6721 ttgcaaacgg ggacaaagca aatgaccccg aacacacttt accttgggga agtaaagatc
     6781 cagaccgaga agactttttc gggggagatt tgcaaggtgt gatggatcat ttggattact
     6841 tgacggacct tggcatcaat ggcatctatt tttgtcccat atttaaagcc acatccaatc
     6901 ataaatacga tacgattgat tattatgagg tggatcccgc ttttggcgat aaaaaacttt
     6961 taaaaaactt gattgatgaa gcacataaaa gagggatacg tatcatgttc gatgctgtat
     7021 tcaatcatat gggtgtccat tcaccacagt ggcaagacgt tctcaaaaac ggagaaaaat
     7081 cgatctataa agactggttc cacatccgtt ttttcccagt agattcttat cagatgactg
     7141 acttaccaga aactgctgaa aatatcccat atgatacttt tgcttttact ccattcatgc
     7201 cgaaactaaa tacagcaaat ccagaagtgc aaaaatattt gctggatatc gcaacttatt
     7261 ggataaaaga attcgatatt gatggatggc gtcttgatgt tgctaatgaa gtcgatcatc
     7321 atttttggaa aaaattcaga gaagcagtta cagaaatcaa accagacatc tatatccttg
     7381 gagagatttg gcattcctct caagcttggc tgcaagggga tgaattccat gcagtgatga
     7441 attatgcatt tacagattcg attaaagatt attttgcaaa aaagaaaatc acagcttcac
     7501 aaatggtcag tggaatgaat catcagcaaa tgctttacag agatcaggtg aacgaaggaa
     7561 cattcaactt attagattct catgatactg ccaggatctt gaccctttgc caaggtaaca
     7621 aagaattgat gaagtctgtg atggccttta tgtttcttca aaaagggtct ccttgtatct
     7681 attacgggac agaaataggg atgactggtg aagatgaccc ggattgccgc aaatgtatga
     7741 tatgggagaa ggaggaacaa gatctagagc tattcggttt tataaaagaa cttgtttctc
     7801 taaggaaaca gttgtctaag attatttctg aaggttctac tcagtggcta atagtaaatg
     7861 atcgtgaaga taagctctat ttcacacgga aactagaggg acagatcatc tatgtttact
     7921 ttaatcagtc aaaagaacct tgggtagtag aacaggaaaa tgaagtgatt cttagtcaaa
     7981 attgtcagtt gttagaagac gggaaagcag agatacgaca atacggattt ttgatcatgt
     8041 gttgaaaact gttaccggta acaacaagaa agcgtttgca aatgaagatt tgtgcccatt
     8101 ataataaggg tgtaacagca cgttgaatac aaaggaggaa atgaagatga aaaaaagaag
     8161 tgtgaaacag ttgggtttag ggttaatctc tttaggaatc ttaggcggat tagcggcttg
     8221 tggcaatggt aactcttcag cagaagggga agccgatgaa ggaaaaacgt taacagtttc
     8281 cgttgattct ggatataaag attatataaa cgaaataaaa aacactttcg aaaaagaaaa
     8341 cgatgtgaaa atcaagctag tagaaaagga catgttcgat cagttggaat cattagcgtt
     8401 agatgggcct gctggaaagg gacctgacgt tatgatggca gcatatgacc ggatcggtgc
     8461 gttaggacag caaggacatc tggcggaagt aaaactggga aatgaagcag cttacgatga
     8521 aacagacaaa gcccaagtga cttatgatgg aaaaatctac ggagaacctg cagtaatcga
     8581 gacccttgtt ttgtactata acaaagattt agtcgataca gcaccatcaa cgtttaaaga
     8641 tttggagaat ctatccaaag acagtcgttt tgcttttgag tcagaagcag gaaaaaatac
     8701 aggattttta gctaaatgga ctgactttta ttattcctat ggtttaattg caggttacgg
     8761 tggctatgta tttggtgatg atggcacaaa tccttcagat atcggtttga ataatgcagg
     8821 tgctgtagaa ggaatctctt atgcaacaga ttggttcaaa aatgtttggc caaagggaat
     8881 gcaagacata aaaagtgcag gagacttcgc cagccagcaa ttcatgtcca ataaaactgc
     8941 agcaatcatt gacggtccat ggcaagcgca aacatacaaa gaaaacaata tcaattatgg
     9001 tgtagccaaa attcctactc taaataatgg acagccttat caaccgtttg gcggaggaaa
     9061 aggatgggta gtcagcaatt atgctaaaaa caaagacctc tctcaaaaat ggcttgatta
     9121 tgtaacgaat caggaaaatc aagaaaaatt ctttgaaatg gtaaatgaaa tccctgcaaa
     9181 ccaacaagct agggaaacag caaaggctaa aaacgatgaa ttaacaacag ctgtgattga
     9241 acaatatgaa acagctcaag cgatgccaaa catcccagaa atgggcgaag tttggacagg
     9301 tgcagaaaac ttgatgttcg atgcagcatc aggttcaaaa acaccaaaag aatccgcaga
     9361 tgaagcagta aaaacaatca gtgaagccat agaacaaaaa tactgattaa aatgataacc
     9421 gtccattttt ataaaaaatg gaacaggtgc ttcagataaa agtggcaaga gcgagatgga
     9481 aaattatctt gctcttgttg ccattatctt aaaagcagtt tgcggtttag caagtttaga
     9541 gggggcattc agatgcagca agataaaaca agcaatatca acgaaaaaaa aatcacgctt
     9601 tgttcactta ttccagggat ggggcagttt gtaaatggcc aagcttttaa aggtatcgtt
     9661 tttttcgcca tattcgtcct attcattggt cagatgcttc tgggcggatt tcatgcattg
     9721 gtaggattgg ttactttggg aagtatacca atggaagatc attcattatt tattttgatt
     9781 gaaggcactt tgcaattcat cattacaatc attttcttga ttttttacgg tttgaatctt
     9841 ttagacgctt atcgagtagc gaagatgcgt caagagggca aaaaaatcaa tcggacgatg
     9901 aaggaagtca tacataatat aggtgatgaa ggttttcctt atctattgac acttcccgct
     9961 tatcttctca tgatttttac gatcattttc ccagtcctag ttacattgtt cactgcattt
    10021 actaattatg acttcaatca tattccacca gccagtttaa ttgattgggt aggggctaaa
    10081 aactttttta gtatcttctt tttaagctca tatcgtaata ctttttttgc agtcttttcg
    10141 tggaccgtga tttggacttt atgtgcaaca actttgcaaa tcacccttgg tattctcaca
    10201 gcggttgttg ctcaccagaa atttattaaa ttcaaacgta ttttcggtat cattttctta
    10261 ttaccatggg cggtcccagc ttttattact atcatgagtt tttcgaatat attcaacgat
    10321 agcgtaggtg ccatcaatac tcaagtgatt cctttcataa accagcttcc ttttgtagat
    10381 atcagtgcaa tctcttggaa aacggacccg aactggacga aagtagcaat tatcttagtg
    10441 cagggctggt taggatttcc ctatatctat gtgatggtta caggaatctt acaatcgatt
    10501 tcagaagact tatatgaagc agcaaaaatc gacggagcaa atgcatggca gcgcttttca
    10561 aacatcacgc ttcctgctgt cttcctagtg gcagcgccaa catttattac acagtatacg
    10621 gggaatttca ataatttctc catgatctat ctgtttaaca atggcggtcc cggaagcgta
    10681 ggcggaggag ctggttctac tgatattctt atttcctgga tctataaact tacgactgga
    10741 acttctccgc agtattctat ggcttcagca atcacgttga ttatctccgt catcgttatc
    10801 tgtgtgtcat tgctggtatt taaaaagaca aatgcgttca atatggggga ttaacaatga
    10861 aaaaatttca ttcgagtgct cgattccaaa aacgactttc tcactttttt acttactttt
    10921 ttcttttaat actgtcagtc attatcatct atccgctttt gatcacagca agtactgcct
    10981 tcaagtcagg aaatattgca gcttttagtt tagatctttc aagtgactgg actttaggaa
    11041 actttactcg gctattcagt gaaacacttt acgggacttg gtataaaaat acattgatca
    11101 tttctgtcct aacaatgatc gtacaagtga ctgtagtaac tttggcgggc tatacttata
    11161 gtcgctatcg ttttatcggc cgcaaatcaa gtttgatgtt ctttttagtg attcagatgg
    11221 taccgactat ggcagcattg actgcctttt atgtcatggc tttcttatta gaagcgctcg
    11281 ataagtactg gttcttgaca atgatctata taggcggtgg aattccaatg aatacttggc
    11341 tgatgaaagg atactttgac actgtaccaa tagatttaga tgaatctgct aaacttgatg
    11401 gggcagggca tttcaggatt tttttccaga ttgtattacc gcttgtcaaa ccgatgctcg
    11461 ccgttcaagc attatgggct tttatgggac cttttgggga tttcatgtta gctaagttcc
    11521 tgcttcgaac acctgaaaat cagactgttg ctgtcggctt acaaacattt atttctgatg
    11581 tgacaaacca aagagtctct ctttttgctg ctggggcaat tttgattgca ctaccgattt
    11641 gtctattatt cttcttttta caaaagaact ttgttaccgg cctgacatct ggcggaacaa
    11701 aaggatagga gcagagtatc atgcaaacat ttccgttaaa ctatttttcc gcattacgta
    11761 cgccaactca agtgtttgcg ggacgcaaac tattatcatg gcctaaattt ttcttgatat
    11821 ttgtattttt agtttccttg atggtcatgc ctgtgactct tttttatgcc aatcagatcc
    11881 aagcgattcc attagaacaa tttctatccg ttcattcgct gatcgatgaa caaggaaccc
    11941 aaaaattttc tgaacttgaa ttatctgaaa ctgggttaca agcatcgcaa caaacgatag
    12001 ctgtgacccc tgaaattttg gtaggtgtgt ctttatctga gaaacaacaa tccgatcatg
    12061 gaacttttat tgattttgag aaggaacaat gggtgatcca acaaaaggat aaatctggta
    12121 tacgccgcta tacgatgaat tatagcccat ctttccaacc agattctgta cgtacgcccg
    12181 aagatttcca acgattttta gaaagagaat tttatgccag taatcgtcca acaatcattt
    12241 tatcttatag tttaagtctt ggtcttgtcc tatttgtcat gacgagtctc attttattcg
    12301 gggcttcctt tttcttgtgg atgacaagaa aaagccaact ttcatccatt catacattca
    12361 aagagagtgc aaatttgatg ctgaatgtta tgggaatagg ttccatggca gcagcagttg
    12421 tagggtttat ccactttgac tttatcttga tgctaagtgt ccaaacaacg ataactgtct
    12481 tgctgctttt atgggttttt gtcaaaacaa gattcaaaga taagcgtgtc gaatgacaag
    12541 tttaagcgat ttatgcgaca atgaatgtga aaaaaatgaa aaataaagta gtttttgaaa
    12601 aatttctgaa aaaattaaac ttcttagttg attcttttct tttttagtat taacgtaata
    12661 gggtagtttt tttaaggagg aaaatcatgt tagagaaaac tttatacaat caattatttt
    12721 cacgttcttt ctcgctccct gtcgaagtaa attattggga cggtacaacg aaacaatacg
    12781 gagatacgga cactcctccc caaatcaaaa tcaatatcca tgaagaaatc ccaatgaaag
    12841 agattacaag taacgcttct ttggcactag gagaagccta tatggatcat cgaatcgaga
    12901 tcgagggaag tatccaagca ttgatcaacg atgcttatca gcaagcaaac agctttttac
    12961 gaggaaaaga ttatctaaaa tggctgccaa agaaagaaaa acatacgaaa caaaaaaaca
    13021 aagaagatat ccatgcgcat tatgacttag gtaacgactt ttataaactt tggctagatc
    13081 caactttgac ttattcttgt gcttatttca atacacctga agatacgttg gaacaagcac
    13141 aaatcaataa agtccatcat atcttagaca aactctttat caaagaaggc gaaacgttgc
    13201 ttgatatcgg ttgtggctgg ggaacattga tgttcactgc taccaaagag tataatgtca
    13261 aggcaactgg gatcacacta agtgaagaac aatacgattt gattaccaaa agaataaaag
    13321 aagaacattt agaagataaa tgccgtgtat tattaatgga ttaccgcgaa ctaaaaggcg
    13381 aaacgtttga tcatatcaca agtgtaggta tgttcgaaca tgtaggttct gaaaatcttg
    13441 aaggttattt caaagtggta aaagatttat tgaaaccaaa aggaacagct ttgatccatg
    13501 gaatcagccg acaacaaggc ggtgcgacaa atgcttggat caataaatat attttccctg
    13561 gcggttatgt ccctggtgtt actgaattag ttgataatat tacaaaaaac gatttgcaac
    13621 tgattgattt ggaaagctta cgtcgtgact accaattgac attagaacat tggacaaaga
    13681 attttcatca agtccaagat caagtcacga aagaaatggg tgattccttc tatcgaatgt
    13741 gggatctata tttacaagct tgtgcagctt catttgaatc aagtaatatc gatgtgattc
    13801 aatatttgct tgttcaacct ggaaacaatg acatcccaat gcatcgtgca taattgaggc
    13861 aaaagtactg tttaaagact atttaaaaag aagctaagtt ttatgatgct ccagaagtga
    13921 acttttttgt ctaacttcta ggattgtctc taaagcttag tttctttttc tttatatttt
    13981 cataataaaa gtttggttct ttaattttta aacatgacag aaaatttttt cttcgttata
    14041 cttgtaatag aatactattt tataacaaag gggatcgaat caatgaaaaa aactgttatt
    14101 tatactagtt tatttgcagc tttgttttta gctgcttgtc aatcaaatac caatcaaggc
    14161 acatctggga atacgtcttc cagtagcgaa cagcgctctt cacaggtatc ggactcctca
    14221 acaaaagcaa gcacatcttc gtctagccag acaaaaagtt ctagtgaagc aagtactcag
    14281 tcagatatca aagtgtctgc agctcgtgcg atcgaattgt tccaagataa atacccagat
    14341 actgcaatta ctagtttgga attagatagt gattggggat cttacttcta caagatagaa
    14401 ggtgtcgatg atcaaaaaga atacgaagca agaattgatg ctcaaaatga aaaaatggag
    14461 acagagaatc cagaaactct agatcgagat gaacaaaatg gtcaaaagaa aaaagaagat
    14521 ggtctggatg ttgataaatt gatcacgatt gaggaagcag caaaaatcgc cgtcgacaaa
    14581 gtaggaagcg gtacggcaac agattgggat ttggataaag atttaggtac tacttattgg
    14641 gaagtcaaag taaaagacgg caatcaaagt acatcagtca agatcaatag ccaaacaggc
    14701 gaagtactgt caactgaaca agatgattaa gaatttcttc ggaaaacaag agttgatttt
    14761 tcttttagtc atgatacact agacaaaata acttgtttga aggagcgatt tacttgagtt
    14821 tagaaacgac ctttgccaat catatctttg ccaatccttt gatgaatgca tctggtgtcc
    14881 actgcatgac tacccaggaa ttagatgaac tggctcattc agaagcagga gcatttatca
    14941 ctaagagctg tacgatcaat gaacgaaaag gaaatcccga accacgctat tttgatgttc
    15001 ctttagggag tatcaattct atgggacttc caaacttagg tttttcttat tatctagagt
    15061 atgctcttgc gtatgaaaaa gtgcaggaaa accaaaatca accgttattt ttttccattg
    15121 ccgggatgag tgtccaggaa aacttggaaa tgcttgagaa aatagaaaaa agtgggttta
    15181 atgggatcac tgaattaaat ttatcttgtc ccaatgtgcc tggaaaacca caactagctt
    15241 acgattttga ggccacttat gaaacactga aagaagtatt cagcatcttt tctaagccgc
    15301 taggaatcaa attgcctcct tattttgatt ttgctcattt tgatcagatg gctgatatct
    15361 tgaatcagtt tccattgacc tatgtcaatg ctatcaacag tgtgggcaat ggtttgtata
    15421 tcgatacaga acaagaagca gtagtcatca aaccaaaaga agggttcggc ggcattggcg
    15481 gagagtatat caagccgact gctttggcga atgttcgcgc tttttataca cgtttgaaac
    15541 cagaaatcca aatcattggt acaggtggga ttcggacagg acaagatgca tttgaacatc
    15601 tgctatgtgg cgcaagcatg ttacagattg gtaccgagct tcataaagaa gggacggaaa
    15661 ttttttctag aatcataaaa gaattaacgc agattatgtc agaaaaagga tatacgtcaa
    15721 ttgacgaatt taaaggcaaa ttacgcacca tttcatagca aagagacgaa ggtcatgatt
    15781 gatcttcgtc tttttttata aaaaacactc gtctgttcat tcctgtgcaa gcgtactcat
    15841 tgtatgttag acgaacttgt gggaaaataa cagaggcaaa aaggaggata atatgaatga
    15901 tgcaacagaa ttagcaaaag agcttaaaga aaaagaattt agttttcaag aactagttac
    15961 ggatatacag aaaaaaataa agaagcaaaa tcatgaattg aatgcgtttg tcgcgcttga
    16021 accattcgag agtaattccc acgagctatt gattccccag tcagaaaaag ggccattggc
    16081 tggtatccca tttcctttaa aaatgctcgg tcaagaaaaa aagggatggc tagctacttc
    16141 aggatctcgt ttatttgaaa cacaccgagc ttctcataca tctaattatg tgcggcaagc
    16201 agaagcgata ggtctagttc cttttggaca aaccaatgcg ccagagtttg gtttcaaaaa
    16261 tattacagac ccagtcatct atggcccagc aagaaaccct tggaaccttg aatgtacacc
    16321 gggcggatca agtggaggag ctgctgctgc agtttcatca ggaatcgttc ctttagctgg
    16381 tgctagtgat ggcggtggtt ctattagaat acccgcttcg ttttgcggat tgattgggct
    16441 gaaaccttcg cgagggacga tgccagttgg tccatatgct tggagaggat ggcaaggagc
    16501 tgctattgat ttcggcttga cagtttctat gagagacacg gaggctttat tttatgggat
    16561 gagaagtatc cattccggtg cgccatatca agtatcgcca gttgagtggc aaacacatcc
    16621 aagaaaaaaa cggttgaaga tagctttttg tgttgattca ccgatccact cacagattag
    16681 tgaagaggca gtacttgctg taaaaaaagc tgtagatttc cttggaaaag aagggcatga
    16741 tttgacagag attccttacc cactagatgg tcgtaaactg atcacgtctt attatcagat
    16801 gaatggcgca gaaacagcag caatgatccg ctcgattgaa acaggcatcc agcgagctgt
    16861 cagaaaagaa gaacttgaac ccatttcttg gactttgttc caatatggtc agaagctctc
    16921 tgccgctact tacatcgatt ctctgcatgt atgggatgaa gcagcaattg tcatggaaaa
    16981 cctacatcaa gaatacgatc tattcctttc accaacgact gcatttaccg cccctaaaat
    17041 cagcgaagat ttgcaaagtg atgcgataag agcacagatg gaacacatca gtgattgttc
    17101 tgaagtagaa ggtttagacg ttgtttcttc catgtttgag gagagcttga agatcacacc
    17161 ttatacacag ttggctaatc tgactggaca accagcaatc agcttgccta ctcatttgac
    17221 aaaagaaaac atgcctctag gaatccaatt tatggctgcc agaggaagag aagatttgct
    17281 atttcaaatc ggctatctat ttgaagcaaa tcatcagttc catttaccaa atccatggaa
    17341 atagcagaaa caaggagaag aagaatgaag aaaaaaagat tgtttgctct aataactagt
    17401 gcctttttta ttttaagcgc ttgcggtaca gctgccgcta atcaggaaga taacagctgg
    17461 agtaatgtag aaaaaaagaa agaattagta gtagcgactt ctggcacact gtttcccgca
    17521 tcttattata atgatcaaaa tgaattagtt ggttatgatg tagatttggc aaaagaggtg
    17581 gctaaacggt taaatgtcaa aatctccttt aaagaataca acgtggatgg acaaatcact
    17641 tctgtttcaa aaagagaatc agattttgct gcgaacgatt ttggtatcac aaaagaaaga
    17701 gaaaaaaaat tctccctttc agagactttc aaatattcat ttgacagtat gatcgtacga
    17761 aaaaaagatg attcaggaat ccactcactt gaagatctta aagggaaaaa agcagctggc
    17821 gaacccaata ctagctatat gcgtttagcg gaaaaacacg gagctaaatt agtcacatat
    17881 gacaatgcca cgaatgatca gtatttaagc gatgtagcaa atggtcgaac agatgttatt
    17941 ctaaatgatt attatctgca gaaaatggcc gtatctgctt taccagatat ccctgtaaaa
    18001 atactggaga acgtttattt caatgaaaac gaaagtggtt ttttattgaa agaaggaaat
    18061 gatgctctga aaaagaaaat cgatggcgta ttaaaagaga tgaaagaaga tgggacgaca
    18121 aagaaaatat ctgaaaaata ttttcaaact gatgtttcag tcaaaccgaa agaaacgatg
    18181 gctgagtcgg tgagtgagtg atgtactttc ctctaattga tttttccctc atgtggactt
    18241 cacttccttt cgtactgcaa ggcttggtat acacattagg gattggcttc gtgtcgtttc
    18301 ttttaggaaa tctggtaggg ttgcttttga cagttttagg tcttttggat tggctgcctc
    18361 tgaatgtatt tattcgtttt tatttatcgt tctttagagg cattcccgca ctagtcctgc
    18421 tgtttctgtt atattttggg ttaccttatc aattaagtgc cttaacggct tcagtcatct
    18481 gctttacgat taccagcagt gcatttattg gtgaaatcta tcgaggatca ctagcaggcg
    18541 tttcttcagg tcagtgggac gctggtttag cttctggttt ttctttcttt ggggtattga
    18601 aatatatcgt tttgccgcaa gcttttcgaa tctcagtacc ggcactcagt aacgtagcta
    18661 tggatctagt gaaaggtact tcgttagcag caacaatcac agtgccagaa atatttcaaa
    18721 aagcaaagat cataggcggt cggacattcg attatttttc tctctatatt ttagttgcac
    18781 tgatttattg ggtcatttgt atcgggatcg aaaaagtgca acagtattta gaaaaacgtt
    18841 ctatgcatca gtcaatctga aaaatagaaa tggtgctagc gtggctggca ccatttctat
    18901 tttttgtttc ttgtatattc tgcctagtat tttaaaacca tttcatgtaa aatgtaaatc
    18961 gaaaatatgt tcggaggtag aaaacatgaa gaccccgcta accatcgaaa tagaatcctc
    19021 gttataccaa tattgcatcg agcaaggcgg cttggttgta gaagaagtca cgatgcctga
    19081 tgaccaagga attgtagata cgcttagctg cttgacaaaa tttgatggaa gccgagaatg
    19141 gcgatgctat gagctgaaag tgacaaaagc agattttcgc tctacagcca aactgtcatt
    19201 tgttggacat tataattatt ttgttttacc aagtaaacta tatgaagaag taaaaactga
    19261 aatccctaca ggaataggtg ttttagtgta tcgtccgtac acggttgaag aggagatgcc
    19321 tgcctctggt acatttctga ttgcgaaaaa agcagcaaag aaagagcttg ctgtaccgga
    19381 agaagctttg acaaaccgct tcatggcttc tttattcagg gaagtaagaa aagcaaagca
    19441 aatggagcga ggcgtaaaat attttccaac agatcaattg tacaaagaat tgaaaagacg
    19501 ttcagaaaat caagatccat tttctaacga acgatattat gaacggtttt tagaggaaat
    19561 agctagtcag gcaatcaatg agttgcaaga agaagttatc gcgcttcagc atgattatga
    19621 atatttgagg caacaacagc agatccgtcg gttgcctaca gagccattgg agtgaaaaat
    19681 atgtactatc cttatttaag aggaaaacaa tttgacctat tagcattaaa agaagcactg
    19741 tcgcgaggat tgctctccaa taaaatccaa ccagtgatcg aaccagtacg tgattccgct
    19801 acgcttaaaa atgtgatcga attatttcaa aaaaagcacc atccaatcgc tgtgatccaa
    19861 aatccccaag taggacaatt caaattattc gatcagcatg tgcacagttg gacagtcaaa
    19921 gaaaatagct ctgtcgtccc tgcacagatc ttaacgccag aaaatctcag tgaagtactg
    19981 gattcacctc cagcgtttct tgttttcgat ggacagcact acccaaaaga tacagaggtg
    20041 tggaaacagt tggcaggagt tgattcgaaa tttttgatac cagatactag tcgctttcgc
    20101 atctggctgc ccgaaaacaa aattgtgatc cgtgactctt ttcaaacgag aaagcatgtt
    20161 gaaagttatg cagataaaac agatgacttt tttagtgatg actatctttt tttccacgca
    20221 gacggatata ttggattttc tgatttcacg atcgaaggta gccgttattt cgataaaggg
    20281 ttccctagcc gagcgcttgc tatccatcta acgtatattg atgcttacgg caatatacgc
    20341 gtgaaacatt ttgtctctga cagtaacgac tctgctaaag accaagcatt gaagtttctt
    20401 gaagcaggag ataaaatgaa aaaatggatc atgcgtcacc atcatcaact cttgattact
    20461 tcaggaatga ttgaattatt ggctctttac cagcaacaaa aattccctgg actgggagtg
    20521 ttgaaaaagt ggtcacttat gcatcatttg gaacttatta gtcacctgct agatcatcca
    20581 aaagattggc ttaaatatta ttcaaaagtt caggaactat atgaagtgat ccaatgatta
    20641 gataatgtaa atgaaaaagg aatagaaaag aggaaactgg atgtatattc ggcaaacaag
    20701 gaaagaagaa gtagaagaag tggtagaaat cattaatgcc gcagcggatt tcattgcttc
    20761 gcaaggctct cctcaatggc aaaacgggca gaaaccaaca aaagaaacga ttttgaacga
    20821 tattgaaaga aaagaaagct atgtattaat agtggaagat caaattgctg gaacagctgc
    20881 attagtctca agcgtggatc ccgtttacac aaaaatagat ggtagttgga aagaatcaac
    20941 tggtccttat ttatcgattc atcgtgtcgc tttagcagaa agtactcgag gaaaaaaact
    21001 agcaaaaccg ttcttagaac atttgttgac gattgcagcc agcaaaaata tccaagatat
    21061 ccgtatcgat acttatccag ctaatgaacc gatggaaaag accatctatt ctgctggatt
    21121 cacttatcaa gggatgattg aatttccttt tgaacacggc gaaagaaaag catatcaaat
    21181 aatcatcaat tgactataaa atcatctgag caaagataaa gacattaaca ctactgaaaa
    21241 aaacaatcat atgatagcca agaggttgta acagaagtgg ccagcttcaa aaaacaagaa
    21301 agaatttccg aaaatgcttc tcaaattttt gtggaatttt agcgtatttt acgaaatagc
    21361 tgctttctga aactcccttc atcgagaggt cgtgacatag cttttgtcac gacctctcgg
    21421 cttatcctac gagtattatt cgatacattt atatcaataa tccttttttt attttatctg
    21481 cagcttcttg gaacgctcca tcgtttgaca cgactaaaag tgaggggata gctttcaaac
    21541 gtttcaactg tgctaaatct tctacataaa ccgccagtct ggctgaaatc tgttcagatg
    21601 aatctccacg ttttttcagg cgcgattgca atgtttcttt agagatcgta ataaagatag
    21661 gacggactac cgtcccgaaa tactcatata aatgccaaaa accatttgct gtcaaaacgg
    21721 cgtaacaatt tcctactgtc agtttctttt taatttctgc tttactggaa ccatatttat
    21781 gttgagcata ttggtcccac tctgcaaact catttccctt gatcatctga ttgaatgtat
    21841 tgtcagaaac aaaataataa tcctcttgat ctttttcccc aggtcttggt gatcgattcg
    21901 tgtaagtaat gatcttttgt tccggctgaa aaatagcttc tgcgagtgaa gtttttccag
    21961 aaccagaagg accgataaat acatagcaat aattcattgt catttctcct ttgatgattg
    22021 aacaagtgaa aaagctttgg aaaatatttc caaagctttt aaaagtaact ttgctttttt
    22081 gtatattata ccagcaagcc agtcacaata gcacttaaaa agctgactaa agtcgctgtt
    22141 agcaaaattt tcaatccgtg ttttgcgaca agatttcctt tttcttcatt taatcccttc
    22201 atcgcaccag aaatgatccc gatagaagag aagttggcaa atgaaacgag gaagacagat
    22261 ataattgctg ttgtacgctc agtaaaatgg aaattaccat ttgctaattc tgtcatagca
    22321 acaaattcat ttgtaaccag tttagtcgcc atgatactac cggcatccac tgcttctttc
    22381 catgggattc cgctgatgaa ggcaagaggg gcaaagatgt aacctaggat ctcttggaaa
    22441 ggatgccgaa tatcccgtgg aagatagcat tgatcatggc aatcaaagca acaaaaccga
    22501 taagcatagc agcaaccgtg atcgccacat ggaaaccatc aagaatgtat tctcccaaca
    22561 tttggaaaaa tgtctgttct ttgttttctt caatgatcag ctcatcttct ttttcttcta
    22621 atacataggg atttacaatt gaagccagaa tataaccgcc aaataagttc aaaacaaggg
    22681 cagtgatcac atatttagaa tcgatcagtg ccatataaga tccgacgatc gacatagaaa
    22741 ctgtagacat agccgataca ctcatggtga acaaacgttt ctcagatagg aagggtaatt
    22801 ccttttttat ggaaataaaa acttccgatt gtcctaaaat agcagaagct actccattat
    22861 aagattccaa tttgcccatc ccgttgattt tacttaatcc tagaccaaca gctttcataa
    22921 ataatggaag aatcttgata tagcgtagaa taccgataat agcagaaata aatacaattg
    22981 gcatcaatac ggaaagaaag aaaggtgcac ttcctttatt tgctacaccg gcaaaaacaa
    23041 agtcaacacc ttgcccagca aaagcaagaa gatgatcaaa catttttgcc aatacagaaa
    23101 caaccgctgt accaaaagtt gtacgtaata atacgaaacc aagaacaagc tgtaaaacga
    23161 acatcacaat tagtggtttc aatttgattg cttttttgtt actgctgatc aaaaaagcaa
    23221 gccctaatgt gacaaataaa ccaataatac ctaataaata actcaaataa tcactcctat
    23281 tccaaattcc gttttatttt acctgttttg caaataaaat actaatcttt agtgaaattt
    23341 tcattacttt tttagagtga aacgcattca ttttccatta gaaaataaag cgaacaaaat
    23401 aaaaggttta aaaagtgtaa cgtcttagta aagtctataa aaaaacgaaa ctatcgatta
    23461 agctaatagt ttcgttttca acatttataa gtaaactttt tctattggta atatagattt
    23521 tcggaagggt agacaggtgc gcaggtaata tcgattccta gttttttcaa tgtttgttca
    23581 tcatttttat tgaccatcac agtagagtgg gcttgtgctc cctcaagttc agctagttta
    23641 tcataagcaa cttgagcgat tggattcgtt actgcactga ttgataaagc gatcaagact
    23701 tcattgatgc ttagtgcatc caaagaggaa tgaagtgtct ttgatttcaa tccttgtatt
    23761 gtttcaagaa ctaatggtga caataaagga atgtcatctg cgatatgggc taaatgtttt
    23821 actgcattta agataactgc cgctgacgca tccatcaatg aacttgtacg cccggtaatg
    23881 atgacttcat ctggtaattc gattgcaatc acagcagaag gatcttttgt aggactttgc
    23941 gcttgaatct tatctgcata gtcatgggca gggactacca cgtttcggtc ttcaggacgt
    24001 aaaccgactt cttccataat cagtttgatt cgattaactg cttcttcgtc gatcaatcct
    24061 tttttgaagt cgcattctgt ttggaaataa cgacggatga tttcttgttt agaggcttct
    24121 tggacaacgg tatcatccac gatcccaaat cctacacgat tcacacccat gtcagtagga
    24181 gattgataaa cagattcttt accagtaatt ttttcaataa tacgtttgat tacaggaaat
    24241 gtctcaacat cccgattata gttcacagct actttattgt aggcatcaaa gtggaaggaa
    24301 tcgatcatat tgacatcttt tagatcgact gtggctgctt cataagcaat gttcagcgga
    24361 tgtttcaacg gaacattcca tactgggaaa gtttcgaact tggagtaacc agctgcatgt
    24421 cctttttggc tttcatgata cagttggttc aaggaagtag cgagctttcc acttcctggg
    24481 ccaggagctg tcactacaac gattggttta gttgtttcaa tatactcatt ttttccaaaa
    24541 ccatcttctg agacgatttt ttctacgttg actggatagt cttcgatttc tgagtgttta
    24601 tagaccttga tgtttcgtct ttctagttta ttgataaaga ctttcgtggc aggctgtcca
    24661 ctatagcgag tgataacgac actgttgatt aataagccat aaccacgtaa ttcatcgatt
    24721 aaccgcaaga tatccatatc ataagtaatc ccataatcac cacggatctt attacgttca
    24781 atatctccag catagacgca aatcagtatt tcagcttgat ctttcaattt ttgcagcaat
    24841 ttgattttcg catcttcttc aaatcctgga agtacacgtt tcgcatgctt gtcgtcaact
    24901 agcttcccac caaattctaa atataatttg tcatagtggt tcactcgttc caaaatatac
    24961 gctgactgct cttcaatata tttttctgaa tcaaaaccaa tttttttcac taggttccct
    25021 ccttaagtac taaagcattt tttcattttt ttgaggattc gtcaactgga agagcgaaat
    25081 tcctcaaaaa aacaaagaaa atggtttaaa ccaacttgtc tagatagtta ttcattggta
    25141 tgatagtttt tgataaggaa ggtgctacat gaaaaaaaga cagaaaaaga aaaacgcata
    25201 taaacactat atccgttcga tttttaccgg ttatgaaaga atgcttgaag atcccgaatt
    25261 ggaacaattg acgtttactt atctaaacga agaaacgcag ctaactcgtg atgagcatca
    25321 gcgcattcat ttcacaacaa gagatctgcc tagtaaataa aaaataagat aaaggtcatc
    25381 ggattcgatt ctgtcctatc agatggaaag actagttgat cgaaaaaaag gctgtaccaa
    25441 attttggtgc agcctgtttt atggagcatt gcccgctctt ttttataaca attttttaca
    25501 gaaaatggta ctattgaagt attaataagt gaaaaacaga tgagtaagag gaggcaagta
    25561 gtatgaaaga gccagataca aatgcaaatt atgagccaga cgaactagag gagacgaatg
    25621 cggatcagga tacaggacaa gaatcgtctt ctttttacga tatcaaagaa aaagcaagca
    25681 tgaatctttc ccaattatta gaagacttac aggcttttga aaaagctgtc cgcgaagaag
    25741 atatccctga gatctaccgt ttatacaatg gccgattgaa tgaagaatta aaaagaagct
    25801 caaatgaaaa tcacgagatc gatcaattgc tgatgcgtaa gatccatgat cgttttttac
    25861 aggaatttcc ttttatggaa caagtagaaa acatctcgcc aacgatgagc tattacaaaa
    25921 tcggcactta ttaccatgat cggcccacgg tcggtattga tgcaagtctg ccagaaattt
    25981 ttgttttgcc taagatcgat gaagagtggg agaaatattc ccaaccggag tcaacggact
    26041 atgacaaaaa aatcaatgag ctagacgcaa aggtgatcac tgctcagaca gaaattgaac
    26101 gtttagatga acaaataaaa gaaatcaatc gacaaatgac tgatttgaac gacaataaag
    26161 gattcttgaa tcgtaaaaaa atcgaggaag aaattcaaga attagagaag aagaagcaag
    26221 tactatctaa tgaaaaatta ggctggcttc cttatattga gacgcctgaa acaatccagc
    26281 agcaaaaaga agcattgaag caagaagcaa gagcagacca gcttcgtgca gccatcgtag
    26341 aaaaagaaca aaggcaaatc agacgctatt tcggtagcaa agagggattc ggtcaagcaa
    26401 tccatgaatt cttgatgaat tatcttggca atgagaatcc taataatcaa tctaatgaag
    26461 gaggttccga gtatgaatga acacgatgaa gcagaatgga caaaaaatga agaacaaaca
    26521 gaagatcatg aagaaacgaa cacttcattg gaagaacaaa caagtaatcc tctggatgac
    26581 acagaagaaa tccctatgct agaggaaaca aaaatacacg aaaaagaaaa agtaactccg
    26641 acgaatacga atactgcaga aaacacccat gcttacgaaa taactgaaaa tcccgggtat
    26701 ttagaaggaa aagagcaaga tcatcaagat atcgtcagtt tgattgaaac agcggaagca
    26761 gaattagcgg atttacgtat gagaaaagat ttgatcgaat cagattttcc acaattatta
    26821 gaatcatatg atcgttttat attgaatgaa gaaccaatct ccacgatcgg aaagacaagc
    26881 ctgatcgaat acttcacgta tgaattactg aaaccgttga atgatattgg tctggaacgg
    26941 tcaaattcag cttatgacac atggaaaaca agacattttt cgatcaatta tacgttgaat
    27001 ggacaaaacg aattagtctt ttcctttgta ttaccgacca tcgatcaacg ataccaagtg
    27061 gaatcgatga gtctcctaaa cgtatcacca gagacaatgg aaatcaatgt ccaagatgac
    27121 cgggtgcttt ctttgatccg acactggtca gtggatcgag tattctctgc agggcagatc
    27181 acgatattca atcacaaatt gaatcaatta ttagcccatg caagaacact tggttttacc
    27241 gttaatcaga cattactaga caatacgaag ccacttcgtt tgaatcttca gagcgaattt
    27301 gaattgacga atgaagtatt ggatgatatt tttatcgtgg caatgaaaaa tccatcctac
    27361 gatatggaaa agatcaacga aactacttat caagtacgat tagataaagg acagtcttta
    27421 acgataacaa caaacgaaaa agatcaaaca gtcttgtcga tctcatctgg agactaccca
    27481 cgatctgtca ttgatttctt tatcaattac gaattcttgg tcccattaat ggtgagaaag
    27541 tcctgattgg ataagttgga ttaccagatg tcattcagat aattctgttc caaaggactt
    27601 gcggcagtta tccagaataa ttgtcacaag tcttttctgt atgtactaaa tggacgtgaa
    27661 taacttgctg atgaaggagg gatttcatta ggaatgtcca caaataaaat agcacagctt
    27721 agcttattgt ctgctgcttg tgtagcaggt aggatcgctt ttcaatttat tccgaatttc
    27781 cagccaatga ctgctatttt tttatttata gtactttact taagcttaaa agatgcgtta
    27841 attgtcatga gtctttctat tgcgatcagc agtttttatt ttggtgttgg accatgggtc
    27901 atcggccaat ggatcagcta tactgttgtt ctgagtttgt tttcaatcgt ctgcttgaga
    27961 aaaacagtgc aaaggaatct atggttaaag ggagtctgtt tttttctggc gggcatggtt
    28021 tacggaatag gtatgaccgt ttttgatacg ctgttatatc gtttgcctca accttggatc
    28081 tattaccttc aaggggtgtc gtttgatttg atgcacagta taggcaatgt tgtgttcttt
    28141 cttgtttttc tgccagtcgt caaacgtttt tacaatcatt caggaggaaa aaatgaaaaa
    28201 aataatttat agtttgcttg caatgagtgt cagtatactc attttgacaa gttgcagtac
    28261 aacctataca caatcggctt ccaaccaaaa ggaggaagta aaaacatcgc aaaaagcaac
    28321 aatcacgtta caagaaaatg gtcaaaactt ttcggaaaaa gaagtgttct tcaaaaaagg
    28381 cgatgattta ctgagtattt tgaaacgaaa ttttgaagtc aaagaagata acggcttcat
    28441 cacttcgctt gaagggcact cacaggatga gaaaaatcaa ctctattgga tgtttactgt
    28501 ggacgggaaa agtgctacta ctggtgccaa agagatcaaa ttgaatgatg gacaatcggt
    28561 cattttcaat ttgtctaaac tataagtttc ataaaaaact gttaaagggc gaatattgcc
    28621 ataattcagt gagttcagtt gatgaaagaa ggaaaataac atggaaaatt ttaaaaaaag
    28681 cggaatgttt tccgtaattg ctgctctagg ggctaatatt ctagttgcta tcagcaaatt
    28741 tgtcggctat gcaatcagtg gcagtgccgc catgctaaac gaaagcatcc acagtatcgt
    28801 tgattgcagc aatcagattt tgctgttatt cggtgataaa caagccaata aaggacagag
    28861 cgagttgcat caatttggtg aaggacgagc aaaatacttt ttcagtacaa tcgtcgcaat
    28921 gatgcttttc ttcggcggtg gggcacttgg ggtgatggaa gcttttgaaa agttgacgca
    28981 tccatcccat gaagtgggta atccgatcat tgttatagca atactgttat ttggtttggt
    29041 aatcgaagga agttcgttac gcgtggcaat gaaagaaatc aaagaattga atactgaaaa
    29101 actttccacg atgcgctttt tgcgcgaaag tcgacacagt gaaatactga ttatttttac
    29161 ggaagacttg tgtgctgtaa tcggtttggt cttggcttta gcaggaacaa tattgactta
    29221 tgcaaccggg attgctgcat ttgatgcgat cagcggattg ctgatcggtt tcttgctgat
    29281 gggagccgcc attttcctag caaaagaatt ttatagtctg atcatcggtg aaagcgtgac
    29341 agcaagcgat ttatcaaaaa tcaagttagc ttttgaacgt cctgagatca gtcggctgat
    29401 cgatataaaa acagttcatt taagtccaac ggagatttta gttgcagcaa aagcagatat
    29461 cgttggtaac aaagagaatg aagcttactc ggtcatcaat gatattgaaa aaaatatgcg
    29521 tcagtctctt cctgacaaga agatgtatat atacattgaa acagataaat atgacccaaa
    29581 ttattctcgt aaataaaacg tttattcatg aattgaagca aaaatgagaa aaatataatc
    29641 ttgtttttaa ataaaacggc tacaatttag cgaatttatg ttacaatgag tccgctgttt
    29701 atttgttgta attaaaggag atattttaat gaacaaaaag aaaagtgcgc catggcttca
    29761 agctttgcat gcagtgatgc cactttgtat aagctatata ccagtaggat tagcttgcgg
    29821 agtattgttg caaaaagttg gatttgatcc tctcttatca ggactgttat cgattctggt
    29881 cttttccggt ggcgcgcagt ttttagttgc atctatgctt acgacgcaag catcatttgc
    29941 aacgacatta ttgatggttt tctttttaga attacgctat acattattag gagcaagtct
    30001 tgccggtttt atgaaaaaag aaaagcggac gtttttggct gtcttttctc aatcactgaa
    30061 tgatgaaaac tatgcggtga actacttaaa gttttcaact gacccttcct ggaataaaag
    30121 aaaggcttta tatgtcaatt ggttctcaat gactgcatgg gccggtagca atatgatcgg
    30181 taattttttc ggctcggtga ttcgagtaga tgctgacctt gtccattttg ctttgacggc
    30241 catgttcatt ttcatgttta tcatgcagat gaaaagcgca ctactgatct ttacaggttt
    30301 attctcaggc gtattagggg tcgtattcat ggtattattc caaaatactt tcggattgat
    30361 tgcagcaaca gtcattgctt catttgccgg atttatgctg gagaaggcat tcaaaaaaga
    30421 aaaagctaag aaactaagag accgcggtaa agatgtcaat gagccgctct tcaacttccc
    30481 ggaacaggag gccaatcacc atgactaaac tatatttgct acttttagtt gcttgtctgt
    30541 tcatcgtggc atatatcccg cgcttgttcc cgatgcttta ttttactcat cgaaaagtgc
    30601 catcctggtt cagtgactgg atgaaatatg taccggtggc attatttgcg gcgttagctt
    30661 ttaaagatgt tttcattact catgaacatc tggatattgc ttggaatatc aaaatagctg
    30721 caatgattct agtagcaggc gtggcttaca aaacacgttc gatggcgttg tcagttttga
    30781 caggacttgc gtctgtcttt ttgctgtcta tgctttaaac tgttttattt tattaaaaac
    30841 agagattgcg acataagttg ttcaaactga tctatccgaa ctgcatttgc aacagtcaat
    30901 tctggatgca agtttattcg tagggataaa aaagattagt gttggcttat gatgcaagct
    30961 cttttttatt ttaggaatta gagagggatt tgtccttcta tgattttttg ctataatagg
    31021 gaagatgttt attttatgaa agaatagttg agggaattta aatgaataaa aaattgatgc
    31081 aaggaacatt ttggctaacc tttgctaatc ttctatgtaa agttttaggg gtagtctatt
    31141 tgattccctg gctaagtatg atggggaaca atcaagatgg tatgcttgct acgacattgt
    31201 ataatgttgg ctatcttcct tatggattgt tcctgatgct tggtacagtc ggatttccaa
    31261 atgcgattgc aaaaaaagta gctgtagcca caaaagaagg agataacgct gcttgtcggc
    31321 tgatttttag aagtacgatc aatatcatgt ttgtcatcgg tatcgcctct gctgcactga
    31381 tgtatttatt tgctccttta ctagccggaa tcagtccgat tgctaacgtt aataatggga
    31441 tactagctat cagaagttta tgcccatctt tgattgctat cccaattctt agtgcaatga
    31501 gaggatattt ccaaggaaaa aatgatctgc ggccttatgg aacttctttg attatcgaac
    31561 aggctgtgcg agtgatcgtt atcttggctg gtacttatta cttgcgcgta ttgactgacg
    31621 gaaccatatt agaagctgta ttgatcagta cagtcgcttc tttcttcggt gggttagctg
    31681 cgattatcca tatgtatatc gtcggacgca gaaaagactt cttcgaactt aaagattttt
    31741 tgatttccag ccgttatttc gaaagagaaa accgatctgc ctctgtctct atcattcggg
    31801 aaacactacc gtttattttt gtcggctcag tcattactat cgttcaaatg atcgaccaag
    31861 taacgatgaa accactgctt cactttttcc gtccagagat cgcagatcag caactggaat
    31921 tcttattcag ccgtgcttct gtcaatccaa ataaattgac attgatcctg atctccatgg
    31981 taggaacagt agctataagc agcttgccaa tattaagcac gatgaagaaa aaagatcgga
    32041 tacaaatcga aaaaacagta ggagatagtt tttcgattgc gttattgatt cttcttcctt
    32101 cattgactgg aatgtcttta ttagctggac cactatacac attgtttttt ggctatgatc
    32161 ctgaaagtgt cggatatttc caaatggctt tgctagcttc tttgtttttc tctttattca
    32221 cgatcctatc tacgatgctt caatctttga atcaccatcg ttttgcgatc cgattaactg
    32281 tagaagcaat tatcctgaaa gtcatcttcc aagtaatcgg attaggatta gttggcggat
    32341 acgggatgag cttatcaaat ggcttagcat tcggtattgt ctttgttcgc ggctatcact
    32401 ttatgtgcaa agaataccga atagcacctt tagcaaagat cagcaatttt ttcttaaaaa
    32461 ctttccgctc caccctgatt atgcttgcag tatgttttgc cgttttcttt ttgctgaatc
    32521 agcgattgtc aatggaatca aaaagctatg caatgattta ttgtgtggct gtaggaagta
    32581 ttggcggctt agtattttta ttttctcaat tcggaaaaaa tgggtttcga atgttgaaaa
    32641 atgttaaaca tcatagcaga agcaaagaat aggttaacta atcgtttttt gctgtcactt
    32701 gaaaaacggc aactcgtatg attacgcttg tcgtttttct attttccaat tttagtttca
    32761 tatctaatct tacttatgat atagttataa ggtctggaaa aattctttga aacgaggaaa
    32821 ttaaacacat gaaacttgca gtagttacag atagttcggc atatctgccg gaacgtatca
    32881 agaaccatcc cgacttattc gttattccaa tccctttgat tatggatggg aaaatttata
    32941 acgaaggaat cgatatcgaa gcggatgaat attatggact tttaaaaaac agtaaagatt
    33001 ttcctactac ttcacaacct gtcgtagggg aagttttggg tttatatgag gaattgaaag
    33061 agaaagggta tgacacgatt ttaagtatcc atctttcttc agggatttct ggctttgtca
    33121 acacattatt tgcgatgaaa gatgacatca gcggagtaac ggtcattcct tatgactcta
    33181 agatcactag tatgccgatg ggacacatgg tagaagctgc cttagactta aatgatgaag
    33241 gtcagtccct tgatgaaata cttgcacata tcgatcgaat ccgagataat acctacgctt
    33301 atctgattgt agatgatttg aacaatttag tacgaggagg acgtttgaca aatggtgcag
    33361 cgctgattgg cagcttatta aaaatcaaac cgatcctgac attttctgaa gggaaaattg
    33421 ttttattcga aaaaatccgt tcaagtaaaa aagcatttgc tcgagctgaa gaaattattg
    33481 gtagaagaaa taacgaaata caaccgccag tcaaacttta cgtgatacat gcaaataatt
    33541 tagaagtagc gattgcggag aaagagaaat tgcagaaaaa atatccaaat gcagaaatcg
    33601 aaattggtca tttcggacca gtcattggta cacatttagg tgaaaaagcc atcggcattg
    33661 ccatttcagc gcaataattc cttttattca aaaaacatac caaaaatatt ccaacatcat
    33721 cttggacttt ctacatgtta ctaatgagcg tgtaaaagaa cgagatggtg tttttatttc
    33781 tatcaatgaa gctctttttc tttgcaatcc tcacttcaat cctctataat gtcgcatata
    33841 gtttagtaaa tgagtaaact actaatttac ttatttacta aaaataaaag gatgaggagt
    33901 taaaataatg gaaataccaa caaatttaaa acataaacca gtaatcgttg tagaagacta
    33961 tgataaagtt gatggaagaa atgcattaaa tacggatgca aaagggcttt cactgggcct
    34021 tgctcagtgg aatgatcgtg gaaaagtcga tatctcaggc aaggtatggc gacatactgg
    34081 agaaaaatgg tcacgacagt cggaagaatt accaatcaca cgtattttag atttagcgat
    34141 cttagctgca caaggaagtt tatacttcca agatgcttat cgttatgaaa aattttatga
    34201 tccagaaaat ccagtcgtag acatcattgg attacaaggg aaccgaatga cggtagaagt
    34261 agataccaaa aatccaaaga tcgatgaaga catcattttg tattacgata cactacaaaa
    34321 agacggagaa ctgattgggg aacgattccg tattttaaaa cgtcttttag atgagctagg
    34381 atattaatag acagagatga tcaaaagggg aagagaagca tggagaattt ctcactaaca
    34441 gaaaaccgaa ccttttcacg gctagaaaag caaatgatct ggaagaaacc gaaaagccat
    34501 cggacaagtg cagaagaatg gcggatcgcc tccgaaatca aagggaattg gcaagatcct
    34561 gaaatgaaaa tcagtaacgt acttttggaa ggagatgccg ggtcggggaa gacacaatta
    34621 gccaaagcac tatctgctga tctgcaactt ccctatacaa aagtgacctg ttttgcagat
    34681 atggacaaat cagatgtttt cggagcctta ttacctgtag tagattctaa taaggaacaa
    34741 gaccaagaat tactagaagc tatttaccaa acagatacac ttgaagctgt actgacactg
    34801 atccagaacc attacggcat tgatcctttt tctgctaaag aaaaattagc agagctagtt
    34861 caaaggatcg aagctgatta tccagatgca atccattaca gctattaccc ttctgaaatc
    34921 gttcgagcga ttgaaaaagg atatttatta gagattcaag agccgactgt catccgagat
    34981 gcctctgttc ttgtggcact taactcagcg ctcgaaccaa atgggatgtt gaatttaccg
    35041 acaggaatca ttcgtcgcca tccagattgc gtcattgtga tcactacaaa tcgaaattac
    35101 caaggaaatc gcccattaaa tgaatcttta agagatcgga tgcaacatgc ggaaaaaatg
    35161 gatcttcctg ccttagaagt catggcagaa cgtgcgatgg cgaaaactat gatccaaaaa
    35221 caagaacttc tcttaaagat ggcagagatt atccgtttgt tggatgaaac tgcaaaagct
    35281 aacgccatca aaggtgtagc aggaatgcgt tcttattttt attgggcaaa tacctataag
    35341 caaggacaag atttactcca gtcgatttat ccaaaagttt tgtataaatt atccactgat
    35401 tcagatgaat tggcaattct tgaacaagct ttgtctgaca gtggattgct actggaactt
    35461 caggacatat tgagaaaaga aaaatggggt catcctcaat ctgagcatac aaaaggaaga
    35521 accattagcg ccgaagaagc aaatgaacga aacatcactg aaaatgctga agaaattctg
    35581 cgcttagatg aaaagaaatc gttgtctgct gaagaaccaa atgagggaga agagaaacaa
    35641 gaagaaacaa tagaaaaaaa cgagattcct cctcaaatgc aaaaagaacg cagtagcgat
    35701 atcgaaggaa aaaatcaaaa agaacaacaa acaggagatg attctcaaga aatgtctgct
    35761 tctgacatgg aagcacatga taaggaaatc aagaagcaac tgaataaaga agcgcgacaa
    35821 atcatgaagg aaacgattca tgaaaaagaa ggtctcatcg ttcaccgacc agctttccaa
    35881 ggaaaaacgc cggaagcacg tgagctacac atgcaagtaa tgccaatagt agaatcactt
    35941 agccggcaaa ttttagagtt actggataat gaacaaacag agacgtacca aaaaggaaaa
    36001 tacgaaggtc agcggttcaa tgcaagcaaa gtagcatatg gtgatttgag aaattttgac
    36061 aaaaagaacc cgccacatga gcaaccttct ttagcggtag ctgtacggat cgatgaatct
    36121 gggtcaatga tacgggatga tcggatcgaa gcggcaaaaa aagcagcgat tgccattgcc
    36181 gaattttcta agaaagtcga tatcccattg ttgatttacg gagatacagc agaccgttcg
    36241 tcaagagaaa agacttcttt gttttcttat aaagaatttg aagacggatt tcaatggtta
    36301 aatgaaaaat tagtcactat gaaaccaagg caaaacaatc gtgatggtgc tgcacttcgt
    36361 ttagcagcag aaaaattaaa cagacaatcc gcaacaacca aactgttgct taatattagt
    36421 gacggtcagc caaaagcgtt gccggattat acaggcaaaa aagcaaaaga agatatccag
    36481 tctgttttga aagaatatga acgtcaagga atcctatttg tctccgcagc tattggccaa
    36541 gacaaagaag aaatcaagtc gatctacggt gaaagtcgtt ttgtagatat tactgacttg
    36601 aatgaatttc ccaagcaaat gattcaactg attgcccgct atctatgata aaataaagct
    36661 caacgaacgc acttagcaga ttcgttgagc ttttcgtttc tagttaccgg taacaaggat
    36721 acttctaaaa tagaatcggt tataatcaag agggaccgtt aaaggagtga aacgatatga
    36781 tacaagaaca aaaatggtgg aaagaagcag taggctatca aatctatcct gctagtttca
    36841 aagatagcaa tggcgatggt caaggagaca tcaatgggat ccgtgaaaaa cttaattatt
    36901 tgaaagatct tggtatcggc tttatctgga tcaatccgat ctatcaatct ccatttgtcg
    36961 ataatggtta tgacgtaagt gactatcaag atatagaaga gagattcgga acgatggaag
    37021 attttgattt attgctaaaa gaagcacatg aattagggat caaagtgatt atggatcttg
    37081 tcatcaacca tagttcggat cagcatcaat ggtttgaaga atcgaaaaaa tctaaagaaa
    37141 gcccgtatcg cgattattac atttgggtcg acggggtaga tggcaaagaa ccgaataatt
    37201 ggacatcgat tttcggcggt tctgcttggg agtattctca tgaaactgga cagtattatc
    37261 tgcatgtttt tgcaaaagaa caaccagact taaattggga aagcgagaaa ttgaaagaag
    37321 aactattcaa tatgatccga tggtggttgg ataaagggat agatggattt cgtttagatg
    37381 cgatttctca tgtaaaaaaa gatgaatatt cagtaaaagc cacagagaac cctttctcac
    37441 cctttcaaaa tgtctcagga atcgaagaac atttgaccga tttgaaacat gtttttgaag
    37501 agtacgatat catgactgtc ggagaagcaa gtggtgtcac tgcagaagaa ggaccacaat
    37561 gggtagggaa agacggttat ttcgatatga tattcgaatt tgatcatatc catatctggc
    37621 agcaagaaaa agaagggcaa ttagatgtcc ttaaattgaa acttgcatta agcgcttggc
    37681 aaacttcatt ggatggtatc ggatggaacg ctttatatat ggaaaaccat gatgttcctc
    37741 gtgccgtttc agtgtttgga gatactcgtc ccgatttttg ggctatgtct gcaaaagcaa
    37801 tcgctatgat gtatttcttt ttgcaaggta cgccatttat ttatcaagga caggaaatcg
    37861 gaatgacgaa tatgcccttt gaatcgatcg atcaagttga tgcagtagat tcgaaaagac
    37921 tttataaaag gctgcttgcg gaaggtaaga caagagaaga agcattggat atcattcgtg
    37981 aaacaacgag agacaacagc aggacaccca tgcaatggac ttcggaacaa tatgctggtt
    38041 tttctacaca tgaaccttgg ctgatcacta atccaaatac caaaacgatc aatgtagaac
    38101 aacaagaata tgaaccagag tcggtcctgc aattctataa gaacatgatc cgcattagac
    38161 aaaccaataa aggtctgata tacggttctt ataaagaata tcttcatgaa catccacaac
    38221 tttatgtcta tgaaagatat ttagaagatg aacaatatct tattatggta aacttaacag
    38281 aatcacttgc tgactatgaa cttccaaaag aagcagatca gtcctggaca ttactcttgt
    38341 caaattcctc tagcggagaa tttgaagcta aaggcatact agcaccttat gaagctcggt
    38401 tatataaaac aaacaagtaa aacttcctcg ttaaagttca ttagttggac tttgcgagga
    38461 agtttttcag attgctctgt caattactca tttcaataat tcgctctagg accaccgata
    38521 tatttaggac gagagcgaag ggctgtcacg tgagcttgtc caagggtttt caaacgaggt
    38581 ctgacaggta cttggacatg tttgatatgc ataccgattg atgtgtcacc gatatcgata
    38641 ccggcttgtc caactacgtg ttccacttcg acaggatcat tgaactggtc aaaagcagca
    38701 acagaacaag cacctccagc atgtagtgca ggaacaacag agacaatttc gaaatttttt
    38761 gcttctgcca ctgttcgttc tactactaat gctcggttca aatgttcaca accttggaca
    38821 gctagcgcaa tttctttagg atccaacaac tcttttaacg tacggatgat ccattgacca
    38881 acttccgcac tggaattttt tccaatatgt cctccaacga cttcgcttgt gctgcatcct
    38941 aaaacaaaaa gatcaccttt tttcaagtgt gcctcagcca atatgtcatt aactatttca
    39001 gtcaattggt cttttaattc tttttctgac aacttcattt tctaaaacct tctttttcaa
    39061 ttaatattgc ttccgtgctt gaatacgttg aagacttgca gttataggaa aggcaagtcc
    39121 cattccaatc agattttgga agatgtttcc tggtatagaa gccactccag ccggccaatc
    39181 atataaaatc caaccggcga taccatagcc gatgatcata actaagcttc ctaataaaag
    39241 ccctaatccc cacatacgag tagaagattt gctgaaagaa ccagcaacat atccttgtaa
    39301 cccgtggatc actaatgaaa aaactgccca ctgcggatat cctgaaatca aatcgatcaa
    39361 tagaccactg gaagcaccaa caactaatcc acccgttgga ccataaagaa aagcagcggt
    39421 atagatcccg gcctcgcata atgtcacaaa tccattcgta gccggtacag ggatcaaaat
    39481 tgttaatgaa agggcaactg tcaacgcaat cagcatagca tagatcgtta attgcttagt
    39541 tttcatttct cattcctcct ttttgaatac cgccagacca gacacttccg gtatgatcgt
    39601 tcatcagcac gccattagaa atacaatggg caacaaattt tttactttct gctactgctt
    39661 tggtaaaatc tgcccctaac acaagattcg ctgttattgc agaagaaaag gcacaaccgg
    39721 ccccgtttac tgttgtagtg tcgatttttt tatgagaaaa tacctcataa tgatcaattg
    39781 aatagagaag atccacagct tcatctccag ccatccttgt gccgcctttg atcacaatgt
    39841 tttttgttcc tgaagcagtg agttctttag cgagatggat catttctgtt aacgaagagg
    39901 gaagagcttg accagaaagt aaagcagctt ctgccaaatt aggcgtaatc atcgtaacaa
    39961 gtggaaaaag ttcttcgatg agctgcgaaa tatacaattg attgatcaca gcttcagttt
    40021 ctttgaatgc caaaacaggg tctaaaatga ttgggatatt ttgttttgtt tttatgaagt
    40081 ctcgaacaac catgattgct tcacttgaat tcaataaccc aattttgata ccatctaatg
    40141 aagtcatttt atcaatcgaa actaactgtc gtttaagcaa ttccaaagat atagattcca
    40201 gcataaatcg ctcattaata tctagtgccc ctatagaagt caaggcgctt agtccgaata
    40261 gaccaaaatt ctcaaaagtt tttaaatctg tttggattcc tccaccagca aatggatcag
    40321 aaccgccaat tgtcaagatt cttttcataa cattttcctc catgaataag agtataacga
    40381 aattttcaaa aaagaaaaac agccaattgg catattttta tctcatccaa tttaaaaaaa
    40441 caaaaagaaa agaaagacca aaatggatct ttctttcctt aaatagttag gacttttgtg
    40501 attttaattc tttctcgatt tgaagccgtt gcactttcat tgtagctgtt cgcgggattg
    40561 catcaaaatc acggatgata gggacgttca agcgaggaag ttcatggact tgctcccacc
    40621 atcgattcca atccatttct ttatcaggag ctaaagcaat aatcggttgt ggagaatgat
    40681 ttttatccct tacaatcact acttctgcca aaaaatctaa cgaatctaat aaaaagtctt
    40741 caatagctaa tgtactattg atcgtttcga tcaaatccac ttgccgatct ttcaaaaata
    40801 ggtggccatg cttatccata aaaccgtaat ctccgctatc ccaccaatcg ccataaacat
    40861 tttcctggaa ccgtgcatct tccttgtaat aagtcaaagc ccgtccttta gacagtaatt
    40921 gtatatgacc atctgtgttt tcaggtagga cattcccaac tgaatctgta atccgagcgc
    40981 ttgtcatgtc ttccagtcca acgcccatat ctcttgcatc acttgttttc aaactctcta
    41041 gtgtatgagc cttaagaatc attggtccgc attcactttg gccatatact tgaagaaata
    41101 ccgcatcctg tgccatcgac gttcgcaaaa atgtcttcat tgtctggttg tttatagcat
    41161 caaaagtgga gtgatagtat cggatacccg aaaaagcttc tggatgctcc ttagcagtaa
    41221 aacgccactg aacaaagtta ttcggatggg tctctagtgc aattggccgg tgagcttcca
    41281 acatatgaac aacttttgaa ctttgtgcgt ttgctaaagg catcatagga aatcccatgg
    41341 acatcaaaga agagatacca atattgaatc tggaatgcac tggagagatg tgaaaagcga
    41401 ttaatttctt ttccgaaatt tttgtgaaaa ctgttttttg ccatttcgtc cgccatccca
    41461 tagaatgagc agaatgacaa ataagtttag ggataccagt tgtaccagaa gtatgggtca
    41521 tataagaaat ttcatttttg tctaaaaatg attgagaaac agacaaagca ggttgttcca
    41581 ttaaataaag aatag
//
LOCUS       DAZUMK010000013        37493 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000013 DAZUMK010000000
VERSION     DAZUMK010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.013"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            29..1369
                     /locus_tag="ACG808_000679"
     CDS             29..1369
                     /locus_tag="ACG808_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS5046331.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGIILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHAADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFAAYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLIIFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLTLRKGTLW
                     DKGISFFALAVLWLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1564..2007
                     /locus_tag="ACG808_000680"
     CDS             1564..2007
                     /locus_tag="ACG808_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFS5046332.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINAMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILTSKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2269..3687)
                     /gene="nhaC"
                     /locus_tag="ACG808_000681"
     CDS             complement(2269..3687)
                     /gene="nhaC"
                     /locus_tag="ACG808_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFS5046333.1"
                     /translation="MEKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKKYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDILEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPKLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4091..5314)
                     /locus_tag="ACG808_000682"
     CDS             complement(4091..5314)
                     /locus_tag="ACG808_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFS5046334.1"
                     /translation="MTTTLSTNQLKDLIQYRLPEYQALALAIHDHPEVSNYEFYSSAA
                     LIKQLEKEGFWVEKEVAGHRTGFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDEIGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDAALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAALSTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSEKGLASIAIAAELLANTALDLLREPKLLEKIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5346..6185)
                     /locus_tag="ACG808_000683"
     CDS             complement(5346..6185)
                     /locus_tag="ACG808_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS5046335.1"
                     /translation="MKKIIRFAVITFVAIIWLAGCSQGKAATTEKKETTVKLGIIGED
                     TDVWDDVKDRLKKENIHLEYVKFTDYNQPNAALADGSIDLNSFQHQFFLDNYNEEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENKKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDEHARPYVNIIVARKEDEDNKTYQKIVDAYQTEETAKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6202..6882)
                     /locus_tag="ACG808_000684"
     CDS             complement(6202..6882)
                     /locus_tag="ACG808_000684"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFS5046336.1"
                     /translation="MTEFFKTYLPNVYLIPDEFIEATKQTLYMSFWTAFIGGIIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILVLVFLSQFISN
                     QLIKKVTH"
     gene            complement(6884..7957)
                     /locus_tag="ACG808_000685"
     CDS             complement(6884..7957)
                     /locus_tag="ACG808_000685"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFS5046337.1"
                     /translation="MIELRDISVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEAGK
                     IIEKGSSIQIFSHPEEELTKDFIRTATHLDQALETISAHAAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRQKALDYLIKS
                     GVKVTILQKNELDNRIKVIEGGN"
     misc_binding    complement(8016..8254)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(8297..8530)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8607..9278)
                     /locus_tag="ACG808_000686"
     CDS             complement(8607..9278)
                     /locus_tag="ACG808_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5046338.1"
                     /translation="MKGAIFDLDGLLVDTERTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KKWKQSFDILVQAAGSPEKVQEVRAKREEYFYQQLTSGAIQLKPYAKEILTDLKERHM
                     KLGIATTTVTKRATDILDHFQLADYFDTFTFGDEVSENKPSPVPYLTALERTGLEALS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYSVEEKEKLNLLAEGKDLRTVIEMLD
                     KKTEK"
     gene            complement(9366..12014)
                     /gene="mgtA"
                     /locus_tag="ACG808_000687"
     CDS             complement(9366..12014)
                     /gene="mgtA"
                     /locus_tag="ACG808_000687"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFS5046339.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASPNGLSEEQIERSRET
                     YGENAISYGKKTPFIIEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGEYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNDTLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEAAETLDTDTINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVAPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELITGEKI
                     SEMNDHELAQVAEEHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGKFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQQVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKIPFIQSNATFAMLVITTLGIIVGSVLPFTGFGAELGLMPLPGNYWLWF
                     FITVVAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(12357..12531)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            12704..13141
                     /gene="ndk"
                     /locus_tag="ACG808_000688"
     CDS             12704..13141
                     /gene="ndk"
                     /locus_tag="ACG808_000688"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFS5046340.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFATMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKGADAVPGTIR
                     GDYALPGTENIIHASDSRDAATKEIARFFPPFDTNKPSEKAAK"
     gene            complement(13163..14029)
                     /locus_tag="ACG808_000689"
     CDS             complement(13163..14029)
                     /locus_tag="ACG808_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5046341.1"
                     /translation="MYDGELIKKLRVNKKLTQTQLAEGICSKTSLVGIETNSVKKVSF
                     GTLKSFLERMNMTLAEYEWIRNQLDEPKKLKKKRRMLDRIQEDSFDPYKEIANNRRHY
                     KKTEDLYYLVLNLHMSKKTEVSETEFEFLRYECRIIEEYLMQVPEYGQFEVSILADFP
                     WIFSDRFIENNYLKIKKRMRRIAQLNNHEQYLFTFLTNLTLFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKSENHQEFFAVIEYTLGKNQRNSL
                     EKKLLAIAKPSS"
     gene            complement(14263..16341)
                     /locus_tag="ACG808_000690"
     CDS             complement(14263..16341)
                     /locus_tag="ACG808_000690"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS5046342.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVSKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPATLGQTEGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTIL
                     YPVTGTNAEQ"
     gene            16834..18051
                     /locus_tag="ACG808_000691"
     CDS             16834..18051
                     /locus_tag="ACG808_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFS5046343.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEENTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETREKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEASPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(18510..19133)
                     /locus_tag="ACG808_000692"
     CDS             complement(18510..19133)
                     /locus_tag="ACG808_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046344.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWEEYKLTANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLVVLLLTHDGDKVARLKNGKVVTAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     ASSQKNESTELSPSDSGSGQTITGNEENQASIITNESSEGQYQTYGQ"
     gene            complement(19289..20686)
                     /locus_tag="ACG808_000693"
     CDS             complement(19289..20686)
                     /locus_tag="ACG808_000693"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5046345.1"
                     /translation="MKRYQFPKNFWWGSAASGPQTEGRISGDGKGENIFDYWYKNEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HHYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFQLFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKAYHEMDQSGVI
                     GIILNLTPSYPRDESNPEDVKAARIADAFFNRSFLDPAVKGEFPSELIERIKELDLMP
                     EIHPDDLTIIKENTVDLLGINYYQPRRIKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEKGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDRNGFEA"
     gene            complement(20708..21679)
                     /locus_tag="ACG808_000694"
     CDS             complement(20708..21679)
                     /locus_tag="ACG808_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5046346.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMEKISEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISNRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHAIDGLIVFLSEEEWSGFEMLTKMSIPVVALNCPGGPAGIDTIVVNDR
                     ESVAKVIAHHGGQGHKTIGFIAAAQTDYVARERLAGAKEAVQKDPSIHLEIFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            complement(21686..23986)
                     /locus_tag="ACG808_000695"
     CDS             complement(21686..23986)
                     /locus_tag="ACG808_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFS5046347.1"
                     /translation="MYDYPYQNPKLTIRQRVEDLLSRMNIAEKVGQVNQHLYGWECYE
                     KNGDKIELTEKLKEHVKWGGGLGALYGLFRSDPWSKTDYKNGIPAKESWKVANLVQEY
                     VLQHSRWKIPVLLVEECPHGHQGLDGISYPTNIGRGNTFNVALLEQSSRLMAKELAAK
                     GVHLALVSTLDLAKDPRWGRTEECFGEDPILSARFSEAVVRGFQGEIISEKVSFLDQT
                     VEEINKKKDQIGVVLKHCIAQGEALGGHNSGTVTIGRREFSDIYGPLLKSTRNAVGVM
                     AAYNDIDGIPCHINRALFEQTLRKEIGYQGIVMADGVALDRLSDVFTDKKTAAAYALA
                     AGIDLSLWDETYTKIAEAIDNQVVDEKLLDQAVRRVLSVKFLLGLFEQPFANDPKEYW
                     DHLMEESEHLNLETAKESITLLKNDGILPLDQQVKNIAVIGPNAHDVYHLLGDYSAPQ
                     TEDRLKKKIVQEIKTEFPQSKVSYAQGCEVRNQEDQEEKLLEAVTLAQNSDVIIAVLG
                     GSSARNFDMEFLRNGAVSSKGINMDSGENVDVASLSLGGYQEQLIEQLYQLGKPIITI
                     LVQGRPYDLMKIESFTNAIATAWFPGQEGGKAIAQMISGRNNPSGRLSLSYPRNSRQL
                     PVYYYQRAASKQENYYDLSGSPFYPFGHGLSYTTFEYSQMEVKTDIDKVIISIKVKNT
                     GSVSGKTSTLVYVRLIDGVVIQRTKLLKAFRKDELRSQEERLLVFELTSEDFSYMDID
                     DKRHYAKKAVIMVEQQEKEIKCNWQE"
     gene            complement(23988..25313)
                     /locus_tag="ACG808_000696"
     CDS             complement(23988..25313)
                     /locus_tag="ACG808_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5046348.1"
                     /translation="MKVVDRLSEKMLPIVSKMSSQRHLLAIRDSFIVTMPLVMAASVM
                     VLLNALIFSNATVQRILDLSPLAELASIVNNGTMSILAIIVCYNIGWNLATHYIQTGV
                     IDDPSFSPVHAGILSTAVMFILMSLSSTVTLMDGNTAEATGVFLQSLTSSSGLATAMI
                     AALVSTELFIKLAKMKSFKIRMPEGVPPAVAASFNSLVPEIVVILFFSVVVFTLNHTT
                     GLNVPQLIELAIQTPLKAFVLSVPGILFLQFFSDFLWVFGMHGSSILAPIRQAPLLQS
                     IQENMDAFSVGKEIPNIITEPFTNAFGLIGGGGCILPLVIAILWASKRQEQRSIAKFG
                     LTTCLFNITEPIMFGLPVVMNPIYMIPCAIIPSINLVIAYAATSLGIISKTVAAAPWI
                     TPPVIQSFIATGGDIRAAVLTVILIILDVFLFLPFVLAANKAKLAEGGY"
     gene            25544..25969
                     /locus_tag="ACG808_000697"
     CDS             25544..25969
                     /locus_tag="ACG808_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046349.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DITNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGADSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKEATVTFTKIGNYRLKDKDGNIYSL"
     gene            complement(26467..27168)
                     /locus_tag="ACG808_000698"
     CDS             complement(26467..27168)
                     /locus_tag="ACG808_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFS5046350.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDTFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(27184..27624)
                     /locus_tag="ACG808_000699"
     CDS             complement(27184..27624)
                     /locus_tag="ACG808_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFS5046351.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTITSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVLAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFEKLPVETYVGEYL"
     gene            complement(27624..28199)
                     /locus_tag="ACG808_000700"
     CDS             complement(27624..28199)
                     /locus_tag="ACG808_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFS5046352.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(28610..29116)
                     /locus_tag="ACG808_000701"
     CDS             complement(28610..29116)
                     /locus_tag="ACG808_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HFS5046353.1"
                     /translation="MDYKKRVQQMFLDSGVVLKETELERIEYTDFGLGAIETEGLNLF
                     VYENNERYCGKELVLLPYQTCPEHRHPPRDENEGKKETFRCRKGLVYLYVEGKETKKL
                     SCIIPKGQERYYTAKKEIILHAGDQYTIEQNIKHWFQAGATGAVITEFSSSSDDPSDS
                     FTNPAIYR"
     gene            complement(29127..29639)
                     /locus_tag="ACG808_000702"
                     /pseudo
     CDS             complement(29127..29639)
                     /locus_tag="ACG808_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(29778..30479)
                     /locus_tag="ACG808_000703"
     CDS             complement(29778..30479)
                     /locus_tag="ACG808_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5046354.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQEILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(30587..32029)
                     /locus_tag="ACG808_000704"
     CDS             complement(30587..32029)
                     /locus_tag="ACG808_000704"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5046355.1"
                     /translation="MQSRFSEKFLWGGATAANQLEGAYDRDGKGLSVADAMPGGKQRF
                     AIIGSEEFDWTIDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPN
                     GDEGTPNEAGLEFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYE
                     RFAQTVLERYSSKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVA
                     SSKAVKIGHELRSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGE
                     YPAYTARTHKKYGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSE
                     GNLLGGVKNPFLEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDG
                     TIIDDYRIDYLRRHIEAMANAVADGVDLMGYTPWGCMDLVSASTGEMSKRYGFIYVDL
                     DDEGNGTLNRSKKKSFDWYKEVIASNGVNL"
     gene            complement(32043..33908)
                     /locus_tag="ACG808_000705"
     CDS             complement(32043..33908)
                     /locus_tag="ACG808_000705"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS5046356.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(34029..34880)
                     /gene="licT"
                     /locus_tag="ACG808_000706"
     CDS             complement(34029..34880)
                     /gene="licT"
                     /locus_tag="ACG808_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFS5046357.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(35190..35903)
                     /locus_tag="ACG808_000707"
     CDS             complement(35190..35903)
                     /locus_tag="ACG808_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFS5046358.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(35971..36297)
                     /locus_tag="ACG808_000708"
     CDS             complement(35971..36297)
                     /locus_tag="ACG808_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFS5046359.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(36420..36701)
                     /locus_tag="ACG808_000709"
     CDS             complement(36420..36701)
                     /locus_tag="ACG808_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFS5046360.1"
                     /translation="MSLILGVEKQANPYDHKKMLTQQLRELEQDESIHRKAYNQVPPK
                     VEYSLTEEGKSLREILVALSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(36968..>37493)
                     /locus_tag="ACG808_000710"
     CDS             complement(36968..>37493)
                     /locus_tag="ACG808_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFS5046361.1"
                     /translation="LEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRGVFLKFLEKAES
                     EKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITINLLEEKEGSRF
                     SIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELKIEFSKQNHYFF
                     VEMYLASRKSKSAY"
BASE COUNT        10788 a         7998 c         6386 g        12321 t
ORIGIN      
        1 ttgtgaattt aaaagaaaga ggttatttat ggaagataaa aaattgaaaa aacaacttag
       61 ctcccgacat atcaccatgc tagctttagg tggagcgatt ggtgcaggtc tatttaaagg
      121 aagtggcgaa gcgattggaa tcgccggccc ttctgtattg atcgcttttt tgattggcgg
      181 cataatttta tatatcgtga tgaaaggtct gggaaaaatt gttttaagtg gtggtgatac
      241 gcaccacgga ttatctggat tgattcgtcc atatctaggt gctcatgctg ccgattttac
      301 cgattgggtc tactggtcca tgtggattat caatatcatt gctgaagctg ttgcagctgc
      361 ttccttttta cagttgtggt tcccccacat cccagcttgg gtatttgttt ttatgttggc
      421 tgttttaact actatcatca atctttattc ggtacgatta ttcgcagaga cagaatattg
      481 gctggccttt gctaaaatca gtgtcattat cctattgatc atttttgctg cttatcttgt
      541 cggtcagcaa atgttgggta ctggtgtttt ccctaccctt caacaattaa ctgatcatgg
      601 tggttttact ccccacggta tgaagggaat catcagttct ttgcttgtag ttatctattc
      661 ttacggcggt tctgagttga ttgctatcac agtaagcgaa gcagatgatc ctaaacgtgc
      721 gattcctaaa gcaatcaaag gcgtcatggg aagaatcatt tccttttaca ttattccttt
      781 atttttgctt ttgattattt ttccttggaa tactttagca ggcacgaacg ttagtccttt
      841 tgtgatggta tttgaaaaaa tgaatattcc atttgcggca gatatcgtca atttcgtgat
      901 tgttcttgct ctgttctcgt ctatcaattc aggtgtatat gcttcttcga gaattttgtt
      961 cttccgcttg aaagatcgga aaggttcctc tagaaaacta gctgttttaa acaagcatca
     1021 agtaccgcaa cgtgctgtgc tgttttgtac cagtgttctt tatctaggtg tggctctttc
     1081 ttattttgtc ggtgacaaat tatttggcta tctggcaggt tctctttcct atacagtatt
     1141 attgatctgg atcattatca gtgctgcgtc ctttgtactg acactaagga aaggaacgtt
     1201 atgggacaaa ggaatcagct tttttgcatt agcagtctta tggttgattt tccttggtat
     1261 cctattcacg aactccattg gggtaactgt attgaccggt ttgctttacc tgttcattta
     1321 tttcagttat caaaaaaaga atgatgcttt cgtcttgacg aatgaatgaa accaatagag
     1381 aattttattc tatttatatt gaatatctgg agctattgaa aaaaaggagc tgcgacaaaa
     1441 attgttgcag ttcttttttc cgaataaacg gtgttcaaaa accatctgtg cagattaatt
     1501 gattgttctc cacgctcatg acatgataga agaaaggaat gaagaaaaag gagtttgttt
     1561 taaatgaaaa agtattggaa gtttttgttg atgattggtg tttcgacggt agtcatgttt
     1621 ggtatgatgt atttgaatgt ttatgcgctt gatcatatct tcttcagtcg cacacgtgta
     1681 ttcatggcgt tgatgatggg tgcagtcatg gcagtcatca tgttgttgtt catgtggaaa
     1741 atgtatgaaa acaaaaagat aaatgctatg attttagccg ctagtcttct tgtttttgcc
     1801 ggttccttat ttatggtacg aagccaaaca actgtcaacg atacggcgtg gatgaaggcg
     1861 atgatccctc atcactctat tgctatcttg acaagtaaac gagctgattt aaaagatcct
     1921 gaagtaaaaa aattagcgga agaaatcatt caggcacaag aagaagaaat cgacaaaatg
     1981 aaaaaaatga tcaaagaaat ggaatgacca ttatccatcc aaataaaaaa agaggatgtt
     2041 ccagaagtga tcaacttcaa gaaataagaa gaagctactt ctggaacacc gtgaatcaga
     2101 ggttgtgaaa aagctgccct gcatcaagta ataagaacag ccaaacaaaa atagcttctc
     2161 atatttttcg tttggttagg cttattaccg caaatgcaag cttttgaaca ccgtttattc
     2221 gtaaaaggga gtgtgacaaa agtcttgtca cactcccttt ttacgttttt attgttctaa
     2281 ttgcgctgtc ttgcttactg cttcttgcgg tttcacaaat ttctttgaga tcccccaacc
     2341 tgtaaaggca ctgaccatcg aaaacacagg tgaaagcaaa ctgaaaaaca cgaatggcaa
     2401 gtaagtgagt gtaggaatgc caaatgtact tgctacgaaa cttccggcga ttccccaagg
     2461 cactaagtag ttgattactg ttccaccatc ttctagtgtt ctccctaata cagttggatc
     2521 aaatcctgct ttttcgtaag tttctttaaa tgcattacca ggcaggataa cagatagaaa
     2581 ttgttctcca ataaagatat ttactccgat gcttgtcatc aatgttgtaa aaatcaattt
     2641 tggggtgttc gtcatctttc ttgatactcg ttccataacg gttgagatca acccaaattc
     2701 gatcaacaaa ccacctaatg acaatgttag aataatcaat gacacagttg gcatcatgct
     2761 ttcgattcct ccacggctaa gtagtgaatc gatttgttgg tttccagtat ttgaaacaaa
     2821 tcccgattcg atgacccctg ccaacttcgt taaggaagtt tgtgggtttt gaataaagat
     2881 cattcctact gctacagcaa tattcaacaa aatcgtcaaa attgccggta cctttttcca
     2941 tgcacacaag agcattaaaa atagaggaat cagtgaccag atcgaaatag taaagtttgc
     3001 ttctaaaata tcagtcactt cacgaataga tgaaagactt gcggctttac ttgtgtgtcc
     3061 taaaatcatg aataaaaaga aagaaacaac aaatgccgga acagttgacc acatcatatg
     3121 cttgatgtgt ttgaacaaat cggcatcaac aatagctgct gctaaatttg ttgattcgga
     3181 taatggagac atcttgtctc caaaaacggc acctgaaaca atggctccaa caacaagggc
     3241 tggatttagt cctagtgttg tcccaatacc gaaaaatgca atcccaatcg ttgacataac
     3301 agtaaaggca ctccctacag cgctacctac gattgcacaa acaacaaata cagaagggac
     3361 gaaccactgg acactaatca atttaaatcc aatgaccatg agggttggaa taattcctgc
     3421 ttgtatccat acagcaatca atgaaccgac taaaataaaa ataaagattg gaatgatccc
     3481 cggtttgatg ccattgatga tcccttcgtg cagccaatct gtcgggtatt tttttatcac
     3541 tgcgtacaac atgaccattc cgatagctgc caaaatcgtg atattaggtg aaacctttaa
     3601 tccgacaacg ccaatcgcaa tgcctaaaac aatgaccatc aatacaagtg ttgcttctaa
     3661 aaaacttggt gttccttttt tttccatgat aatctctcct tttttagttg ttcttatgtt
     3721 ctaaaaaagt taagatatcg gattactccg ccacaattca tccgcgattc ctcctttata
     3781 aaaataaaaa gacctgcttt caactaagga cgaatgactc cgcggtacca ccttaattaa
     3841 aaacagatct gtttttcact ttcaaacgat tattcaaatg tatggacttt ccatgtaatt
     3901 cgtcaattag gcttgatcgg ttcacagcga ccaccgactt tctgaacaaa acacctaacc
     3961 actactgcgt gaaaagttga cttgtatgaa gtttaccttc cataagatca tttgtcaatg
     4021 aaaaagaaaa tattttttca tttttctgaa aattattttt ataactttcg cgcacaactt
     4081 tatcttttta ctagaaagta gcgccctctt gtttttttct tgctactatt tcttgatgtt
     4141 cttgtttgat tttttctaat agcttgggtt ctctcaataa atctagagct gtattggcaa
     4201 gtaattccgc tgcaatcgca atagaagcaa gtcctttttc acttttagca gcagctttga
     4261 attcttctgt atgtccggca atatatgtct cagagattgc tacagtaggc tgtatcgttg
     4321 gaataacttg gctaacattt cctacatcag aagaacctag acttgttctt tgttccgttt
     4381 ctatttcttc atcaggaacc cctgcttctt ttgcatgttg gaagaataag tcatcgaaag
     4441 aaggcgttac gatgacatca tcgactgcat tttggaacaa tccaaactca aaagtcgtac
     4501 ccgtgcttaa tgcagcacct cgtacgatat tttccacttt ttcatatact ttgtctaatg
     4561 tttgtcgatt tcctgcacgt aaatagaaac gtccagcagc gtagtctggt atgacattgg
     4621 ccgccacgcc tccatctgta atgattccat ggatattgac atcttttggc agttgcaagc
     4681 gtagagcgtt gattccgtta aacacttgga tcaaggcttc caaagcattt actccttttt
     4741 caggtgctgc tgctgcatga gaagcgacac cgtaaaattt gatatcgact ggatcatttg
     4801 ctaaagattc tgtcgttttc ccataacgaa aagctggatg gacacataat gctgcatcga
     4861 catcctcaaa aaaaccttcc cgaacgaagc ttcccttagc tgatccattt tcgcctcctt
     4921 cttcccccgg agtaccatac acacgaattt ctccgccaat ctcatcaatg aattgtttca
     4981 ctatgctggc tgctaatacg gagtaagtac caaataaatt atgtccgcat gcatgaccaa
     5041 tacctggtaa agcatcaaat tctgccaaaa aggcaattgt cggacctggt ttattggaaa
     5101 tataacgtgc atcaaagcct gttcggtgcc cagctacttc tttttctacc cagaaccctt
     5161 ctttttccaa ttgtttgatt aaagctgctg aggaataaaa ttcataatta cttacttcag
     5221 gatggtcgtg gatagctagt gcaagtgctt gatattctgg taaacggtat tgaatcagat
     5281 ccttcaattg atttgttgag agtgtggttg tcataaaaat ttcctcctat gctagtcgtt
     5341 ttttcttatt ttctcccgaa tgtttcccac gcaggtacgc ttgaaccttt tgatgttttt
     5401 tcgattactt tggctgtttc ttctgtttga taggcatcta ctattttttg atacgtttta
     5461 ttgtcttcat cttcttttct tgcaacgatg atattgacat aaggacgtgc atgctcatcg
     5521 actggttcta agaagattgc atcagaagcg ggattaaatc cagcatctac agctacaccg
     5581 ctattgatga ctgaaatatc tacatctgac aaagcacgtg ctgtttggga agcatctaac
     5641 tcagaaatgt ctagattttt tttattctcg gtaatatcag aaacggtagg cgcttggcct
     5701 tttgccttgt ccactttgat caatccagca gtttgcaata acagaagagc tctgccgcca
     5761 ttcgtgacat cattagggat tgcaactttt gctccatctt tgatatcagc cacatctttt
     5821 aacgtattgg aataaattcc caacggtgca ttaaccgtat tcccaatcga tactaaatca
     5881 gttccgtgtt cttcattgta attgtctaaa aagaattgat gttggaagga attcaaatcg
     5941 atcgatccat ctgctaaagc cgcatttggc tgattgtaat ctgtaaattt cacatattcc
     6001 aaatggatgt tttccttttt caaacggtct ttcacatcgt cccatacatc tgtatcttcc
     6061 ccaatgatcc ctagttttac ggtcgtttct tttttttcag ttgtagctgc ttttccttgg
     6121 ctgcatcctg ccaaccaaat aattgctaca aatgtaatga ctgcaaaacg aataattttt
     6181 ttcatttttt ctcctccatt ttcaatgtgt gactttttta atcagttgat tactgataaa
     6241 ttgactcaaa aaaactaaga ccagaatcaa taatgtagcg acaatcgtta catctgtttg
     6301 gaatcgatta tatcctctcg atatagcaag attccctaat cctccggcac ctactgctcc
     6361 agccatggta gtcagtccga tcacattgat gatcgtcaac gctgagaccc gaatgatacc
     6421 aggtaaccct tctaacaaat aaacacgtaa aatgatgccg attgggctcg tgcccatcgc
     6481 ttctgctgct tcgatcaccc caggatcaac ttctagcagt gcattctcga tttgacgggc
     6541 aaaaaacggg acaacgccaa aaatcaaagg gactaaagct gctttggcac caatcgtcgt
     6601 tccaactaag aaacgagtag tcaaagctaa aagagaaagc aagataatga aagggatgga
     6661 acgaatgata ttgatcaact tatcaagtat ttcgaacagc agcctgttag ctagtaatcc
     6721 atttggacgt gtcactacta acgtcacacc taagatgatt ccgatgatcc caccaatgaa
     6781 tgccgtccaa aaagacatat atagtgtttg ctttgtcgct tcgatgaatt catccggaat
     6841 caaatagaca ttcggcaaat atgttttaaa aaattcagtc attttagttt cctccttcga
     6901 tgactttgat acggttatct aattcatttt tttgtaaaat agttactttt acaccagatt
     6961 taatcaaata atccaatgct ttttgccgct gtttggtttc tccagctaaa gtaacgatca
     7021 aacttcctaa gggtgtttct tgtaatagct ctacattacc gtataagata ttcgctgtta
     7081 cttggtactt gctgtaaaga tgagcgatta gaggttcgtt cgtctggttg ccgatataac
     7141 ttaattccac caaccattta tttgcaatct gatcggcaaa agcagcatgg gcactgattg
     7201 tttccaatgc ttggtcaagg tgtgtcgctg tccgaataaa atctttagtc aattcttctt
     7261 caggatgact gaaaatctgg atgctgctac ctttttcaat gattttcccc gcttccataa
     7321 ccgctacctt gttgcagatt tctttgacaa cttgcatctc atgagtgatc aggacgattg
     7381 ttagacctag ctgacgattt agttttttta acagtgcaag gatctgaagg gtcgttttcg
     7441 gatcaagtgc actagttgct tcatcgcata acaacacttt aggttcattt gccagagctc
     7501 gtgcgattgc cactcgttgt ttttgccccc ccgacagttg tttaggatac gcagatgctt
     7561 tttcttccag accgacaagt tccagtagtt cattcacttt ttgacgacgt tcctgttttg
     7621 attttccaga atactttaga gaaaaatcta cgttgtcaaa aatcgttcga ctttccatta
     7681 gattgaaatg ctgaaagatc ataccgatgg cctttctttc ttttcgcaat tcttttggag
     7741 agagctggct taaattcgtc tgattgacga tgacttctcc tgctgttggt ttctgcaaca
     7801 aattcatcac gcggactaac gtactctttc ctgcacctga gtaaccgacg atcccataaa
     7861 catccccttt ttcaatcgtt aaatctacag aatctactgc ctgtatctct ttttcttttt
     7921 gtttgaaaca tacagaaata tctcgcaatt caatcattaa cattcctcct tgtctataaa
     7981 aaaagcatcc tcaagaaaat gaaaatagtc attttcttga ggacgccttt ttgcgtgtta
     8041 ccacctcatg ttcaagctgt gttcacacac aactcctctt caagtaccat catactctag
     8101 caagatatcg gtcgcagccg tcaccgcctt acatctatgc tcgggatgaa ttgctcgatg
     8161 gccattttcc aatagtcgtt ccttgcttct tctcagctac cgaagctctc tgtcaaaggt
     8221 tcttctatcg tacttaccat ttcaatgcaa ataatattca aaaaaaaaat ccttttacta
     8281 acataaacta tgtcagtaaa aggacgacgt atcgtggtac cacctttttt cggaagtttc
     8341 cttccctcaa gcccaacatc tcaaaaagag agattggcgt tcgttttaac ggtcgaacat
     8401 ttccgaattt gcctacttat cgacaaatta ttctccaagg ccattttcag cttttcttac
     8461 ttgactcttc acaccagcca gagcctctct gcaaagtttc caagcttact ttccttctca
     8521 acgaaatcaa tattttgttg ttgggaataa tctacaggag gaaaactgaa aagtcaagca
     8581 tgaaatccaa attatttttt ctatatttat ttttctgttt ttttatctaa catttcaata
     8641 acagttcgaa gatcttttcc ttccgcaaga aggttcaatt tttccttctc ttcgacgcta
     8701 taatttcgaa caaaactgga atcgggaatc aaaacaacgc ctagtcctgc acgtgaagcg
     8761 gctgtcgctc ctactaaaga atcttccact gcaaaagctg aaagagcttc taatcctgtt
     8821 cgttccaaag ctgtcagata agggactggt gaaggcttgt tctccgatac ttcatctccg
     8881 aacgtaaacg tatcaaagta atcggctaat tgaaagtgat ccaagatatc tgttgctctt
     8941 ttcgtaacag ttgtcgtagc aatccctagt ttcatatgac gttcttttaa gtctgtcaat
     9001 atttcttttg catagggttt caattggata gctccacttg tcagctgttg ataaaaatat
     9061 tcttcacgct ttgctcggac ttcctgcact ttttcaggac tgcctgctgc ctgcactaaa
     9121 atatcaaaac tttgtttcca tttttttccg ccccaagcgt caacgatttc tttcgggata
     9181 tgaatatcaa attgctggaa tccccaaagc caaccatccc gatacgttct ttccgtatcc
     9241 actaacaaac catctaaatc aaatattgca cctttcatct atacattaat cctttccaaa
     9301 acaatacacc tagtttagca tgactgatcc aaaatgaaca gaagcataga gcccagccgc
     9361 atcacttata agagttcttg gaatcgacgg atatagattt tcttcacagc tgtcacaagc
     9421 gccagataag cgacgactgt tataaaaaac cataaccagt aattgccagg gagcggcatc
     9481 aagcctaact cagcaccaaa tcctgtgaat ggaaggactg aaccgacaat gattcctaat
     9541 gtagtgataa ccagcatggc aaatgttgca ttgctttgga taaatggtat tttcggtgta
     9601 cgcaatgcat ggagtactaa tgtttgtgtc cacagggatt cgacaaacca tcctgcatgg
     9661 aataatgcga taaaaatcac ttgttgtgag ctatccaatg tatggaaact tccaccaact
     9721 acagcaggac aaataacaaa atacatcaac aagtaagtag tgatatcaaa aatcgaactt
     9781 gttggaccaa aataaaccat gaattttcca atactagatg cattccattt ctttggctca
     9841 tccaaatatt ctttatccat ccgatcccat ggtaaggaaa tgcaagaaac gtcatagatc
     9901 agattcaaaa atagaagttg caaaggcaac attggtaaaa atggtaagaa cgtactggca
     9961 atcagtacgg aaaacatatt gccaaaatta gaacttgctg ttgccttgat atatttcatg
    10021 atgtttccaa aagtttctct tccagaaaga aggcctcttt ccaaaatcgt caaatctttt
    10081 tctaaaagga tcacgtctgc tgattctttt gcgatatcca cagctgtatc cacggaaata
    10141 ccgatatcag ccactttcat tgctggagca tcattgatac catctcctaa aaatccgaca
    10201 gtatgcccat ttttacgtaa agctgtcgtc aatcgtgctt tttgctgagg attcaatttt
    10261 acgaatactt cgtgttcttc agctacttgc gctaattcat gatcattcat ttccgagatt
    10321 ttctcaccag taatcaattc attgatttct aggcctactt gacggcacac agaccgtgtg
    10381 actaattcat tgtccccagt cagaactttt acagctacac catgttcttt taaagctttc
    10441 aacgcttgtt ttgttgtttc ttttggtgga tcaaggaaag caagataacc aatcagaacc
    10501 atgtctgact catctttgac tgaaaattct ccaacaacgc ttggattggt cttttgtgcg
    10561 acaccaatca ctcgtaagcc gtcttcatta agtgattgta cttttgcaag caccgcttct
    10621 tttcgttctt ttgttaatgg tgcgacagta ccaccaagat caataaattt agaaatgttc
    10681 aacatttctt caatcgcgcc ttttgtgatc atctgtgttt tgccagccgg atcagcgact
    10741 acaacactca tacggcgacg ttcaaaatca aaaggaattt catctacttt ttgataattg
    10801 attgtatctg tatctaaagt ttctgctgct tcatcaatga tagccacatc taacaaattt
    10861 ttcagccctg tttgatagta gctgtttaga taagcatggc gcaaaacacg atcatcttca
    10921 tttccttcac aatccatatg atattcaaga atgattttgt cttgtgtcaa tgttcccgtt
    10981 ttatccgtac ataacacatc gatcgcacca aaattctgga tagcatttaa atttttgatg
    11041 actgttcctt ttttggccat cacacttgct cctttgacca aattcgtcgt aacgatcatt
    11101 ggaagcattt caggcgttaa accaactgcc acagataatc caaaaagaaa agcttctaac
    11161 caatctcctt ttgtaagccc attgataacg ataactgctg gagccatcaa tgacataaat
    11221 ttgatcagca gcatggatgt tttgtggatg cctaattcaa aacttgattg gatgggtttt
    11281 tcagatagcg tttctgctac atgcccaaat agtgtgtcat ttccgacaga aacaatgatt
    11341 ccttccgcgc ttccactgat gacattgctt cccataaaga caagattttc ataacttgtt
    11401 tcactttgct gatcgtcttc acatactttc gcatgctttt ctactggata gctttcccct
    11461 gtcaatgctg cttgagaaat gaatagatcc tttgcggaca ataaacgcat atccgctggt
    11521 atcatatctc ctgctgaaag ccgaacaaga tctccacaga cgatttcttc cataggaatc
    11581 tctgtatact cgccatttcg tttgacagct gccgttactt tcacaagtga tttcagtttt
    11641 tcagctgctt gattagaacg cacagactgg atcaacgtca tcgtcccact gacgataacc
    11701 atagtgaaga taatggctac accaaagaga tctttatctc ctggagcggc taatacgtat
    11761 tccgtaatga atgaaataat tcctaatgca atcaacacta atgtaaaggg tgtgatgtat
    11821 gctttcaata tttctataat gaatggtgtt tttttgccat atgaaatcgc attttcacca
    11881 taagtttctc ttgaacgctc gatttgttct tctgacaatc catttggtga tgcatggaac
    11941 aattcaaata attcttgctt cgtttttcca gcaaaagctg catagatttt ttgtgtatct
    12001 ttcttttttt ccatctcgat ctctcctttg tgtccaaaag acagctgttc tcgctgtttt
    12061 tggtcttttg ctattcatcg gatcgataga gaaaatgctg aaaggggctg agaacagata
    12121 gataatagcc ctggcctttc ctctaaatcc ttgtttttcg aatgactgtc atccatttgt
    12181 tctcatctcc tttactaagt aattttaatt catgacttcc aatcattttt cgaatagtta
    12241 ttttcccaac tcgctcacct ccctttttgt tttttcacct atgggatttg cggcaacaaa
    12301 aaaacctctc gccaaatact cagcgaaagg tttgaaaaag caaatcaaaa aaagcttctc
    12361 agatctcatc gttgagtttt gacactgtac aacgtaaaaa gcagatatct gcttttagct
    12421 accttatgaa aagccttatc tcgacaattc ctgttctacc cattggcgtc tctcgacgtt
    12481 tttgggcagt agcctatgtt tgtataggag cctcacctaa caaatcctat tcggttttcc
    12541 ctcgttaata taacaactaa aaaagaggat gtcaaatcaa ttttccatgt ttttcatact
    12601 tttttaaaat acacgcgtta tatttcctgt ctcacgcagt tttatttgtc tcattttcca
    12661 cctcccgcta taatagaatt atagtaggaa acgaggtgct gttatggaac gaacacttgt
    12721 tattatcaag ccagacggcg taagacgcca tttggtcgga agcatcatcc aacgttttga
    12781 agcaaaggga ctggccattg ctgaaatgaa atttgcaacg atgacacctg aactagcaaa
    12841 agaacattat cagcacttga ctgaacgtag tttttttgat gagcttatcg attacatgac
    12901 ttctggtccg gtcgtttatc ttgtgctggt cggcgaggaa gtgatcgata tcgtccgtaa
    12961 gatggtcgga gcaacaaaag gggcggatgc cgttccaggt acgatccgag gagattatgc
    13021 actgccgggg actgaaaaca tcatccatgc ttctgattcc cgtgatgctg caacaaaaga
    13081 gatcgcccgc tttttcccgc cattcgatac aaacaaaccg tctgaaaaag cggcgaagtg
    13141 atacaaatcc taaactgttg gtctaactgc tgggtttagc aatggccagc agttttttct
    13201 ctaaagaatt cctttgattc ttccctaatg tatattcgat cactgcgaaa aactcttggt
    13261 gattttccga tttttcttcc cctaaagcag cagaaatcag tcgttggtaa taatccgcca
    13321 tcaatgtttc ataaatcact gtacttcttt ctttatgcgc taaactccgt ttcatatctt
    13381 cattcacgct acgtgccttt tttaagcgac cattttctat ataaaacaaa gtcaagttcg
    13441 tcaaaaaagt aaagagatac tgctcgtgat tattcagttg cgcgatccgt cgcattcttt
    13501 ttttgatttt caaataatta ttctcaataa aacgatcact gaatatccaa ggaaaatctg
    13561 ctagaataga tacttcaaat tgcccatact ctggtacttg catcaaatat tcttcaatga
    13621 tacgacattc ataacgcaaa aattcaaatt ccgtctcaga tacttctgtt tttttggaca
    13681 tgtgcagatt caaaaccaaa tagtacagat cttctgtttt cttatagtgc ctgcggttat
    13741 tagcaatttc tttgtaagga tcaaagctgt cttcttgaat ccgatctagc attcgtcttt
    13801 ttttctttaa tttttttggc tcatcaagct gatttcgtat ccattcatat tcggcaagtg
    13861 tcatgttcat tcgctctaaa aatgatttca atgttccaaa cgatactttt ttcactgaat
    13921 ttgtctcgat cccgactagc gaagttttag aacagattcc ttccgctaat tgggtctgtg
    13981 tcagcttttt gttgactcga agcttcttaa tcaattctcc atcatacata atagttctcc
    14041 ttttttaatt gaaattaacc cttggctttt ccagaatgct tgccaataag aggaatgatt
    14101 atttcaattt aaattttccg cattcaataa atagatacgt taaaaataaa aaatacacga
    14161 aaaaaataaa aaaatgtttt tctcataaaa aataccaacc ttcaagcatg agactgctgt
    14221 ctaatccctg aaggttggtt tgattgttta ttgatcattt ttttactgct ccgcattggt
    14281 tcctgttact ggatatagga tagtcgttgt tttattgccg ttttttgaaa agacactggt
    14341 cgaatgttca cctttttgct tttcgattgc ttcattacgt tccgcttcgt ttttatagtc
    14401 atattttttc ggatcaatcg tctcaaatcc gtttggcaca taaaaccgca agagattttc
    14461 ctgattcagc ttatctgaaa gacttaacgc agtcttgact ttttcctgat ctttggagat
    14521 ttcctcgtcg attcctgctg ctttcgtatc gatgatttct cctgtctgat tattgtaagc
    14581 ttttcctcct aggacggtgt atttgggcgt cacgaagttc ccatttcgaa aagcaactac
    14641 ttgatcgtgc tgagaagata ataagtctgt accaaaatga agataatctt tatcatcgat
    14701 tcccaataga tgtagtaatg tcggcaaaac atcgatttct ccaccatatt catgattgac
    14761 tgtcccttta gtgtaaccag gcatatggat catcaatggc actcgttgca tctgtgcgtt
    14821 gtcaaattca tcccatgtat ctggatcttt accaagagca cttgctaaat ctttattatc
    14881 tgtattcgag atcccaaaat gatccccgta aatcacaaag attgaatttt ggtaaatacc
    14941 agattttttc aaatacgtaa aaaattgttc caatgattga tccagataat gggccgttcg
    15001 aacataatta tctactgtgc tattgccagt attcaaagat ggaaagtcga aattcttgtc
    15061 atccgtataa taaggcgtgt ggttggttac ggaaagaaat ttcgtgtaaa acggctgttg
    15121 tagatgttca agatattggg cagattctct gaataaatct ttatctaata tcccataacc
    15181 taacgtttca tcggattcat caaaataaga tcgatcaaag aaattgtcat accctaaatt
    15241 tttatataca tgatctctat tccagaaact agctacattc ccatggaaaa cagcacttgt
    15301 atagccttct gtttgaccaa gggtcgctgg tgctgcttga aagacattat ctgacccaag
    15361 ttctgtgaat aatgagcctt gaggtaaacc aaatgtgcca gtttccaaca tgttttcagc
    15421 atcacttgtc tttccctgtc ctacttcatg aaagaaattg tcaaaactga tcgttgcttg
    15481 attctggaaa atactattta aaaacggcgt gacttcttgt ccgtctacct tcatattgat
    15541 caaaaactgt tgaaaacttt ctaaatgaag gacaatcaca ttttttcctt tagcaatccc
    15601 aaatgtttca ggatttggct ctgcataatg tttctttgta tagtctaaaa cttcatcaat
    15661 tccattactg cttgcatgcg cgcgaacact gctagtcatt cctgttttga ttccgtcata
    15721 aatagtgtac gcatcgattc ctagatattt gacaatatag gaacggtcaa atgtccgctg
    15781 tagcaattgg ggacggttgg cttcgctcaa cgctaaattt cctgaaaaaa tcaatcctga
    15841 aagacaggta acagcgatag ccatcggttt gctgacaggg ttgcttttga taggtatttt
    15901 cttgaccttc aaccaaacca gtagcccaat gaacacagcg atatccaacc aatagatgac
    15961 atcatggggt ttcatcaaag aaaaggagct tcctgacaac ccttgtgaca ctttcgaaaa
    16021 gcctaataca gatttgactg taatgaaatc tgtgaattcc cgataaaaaa taatattcaa
    16081 gtacaacaaa agcgtattta aaatatcaat gatcaaaaga atggctaacg ctggctttgg
    16141 ctttttaata tacaaagaaa ggccaaaaaa caataaggta gtcgcaatcg ggttgatcca
    16201 caagataaag tactggatcg taccttccac acctaatgaa aaatctgcat agtaggcaat
    16261 gatcgtcttc agccagaaaa agaaaacagc tgtcaaaacg attgctaaac gattttggca
    16321 caatttttta agttgtgtca cgtatatcac ttctctctat tcacattttt tcttattttg
    16381 ctcctgaaaa atgaaaattt cctaaaaaag ttgttatctt aagatttttt tattctttct
    16441 aaagattatt ccattcattt ttcacagcat aaaatcgttt cggtgtaagc ggttaaaata
    16501 gtaaggagat aaaaatcttg tggtgaccga tttactctat agtattcaaa agtagtttct
    16561 cggtattcgt acaaaaaaaa catactaaga ttagagggga acatcattga cgaatgatgt
    16621 tctcctctat tttctatttt tagattatag tgaagagtga aagaaagttc gaacaaagcc
    16681 ttgaggacta cgagtcccta tcaaaaaccg aacttcttta ctgtcatttt agaatcaggt
    16741 ttacgtatgt tggggccatg agcccgtgta taggaaattg gaatcgaaat gaagcagtaa
    16801 agttctttca acacttaaaa aggaggaaaa caaatgcctt acattcttgc attagatgtt
    16861 tcaatgggac acagctacag tgtcctatac aaagacgaca cctgcttatt tgaagacgag
    16921 atcgaacaca ctcaaagagg atttcatgca ttactcgaag agatccagca cttgcctgaa
    16981 cgtccattga ttgtttttga agcaacgggt atttattcca gaccagtcga aaagttcttt
    17041 caagatcatc acttttctta ctgtttgtta aacccgttag aagcgaaaaa acagatggaa
    17101 gaaaacacct tacgcagttg gaaaacagat aaatcggatg cccatcggct cgcacagact
    17161 catcttaaaa atcaacgtca accaaaagaa gtacaaagca atttttactt agagatgcgc
    17221 gatttagccc gtttttatca agaaattgag aagaaaatca ctcgactgcg catggattta
    17281 cacaactgcc ttcagttgac ttttccagag cttgaacagt tcttttcaaa caggctgacc
    17341 ccttacgcat tgacattgat ccgtcttttt ccccatccag attttgtatt ggcatctact
    17401 cggacaaaaa taaaaaataa actgatcaac gaaacacggg aaaaaatctc cgcgaatcgt
    17461 gccgagcaaa aggcagatca aatcatccac tatgcacagt gtgcctatcc agctgttgaa
    17521 aaagacagta ttcactgtca aaaaacgatc tattacgccg aacttctcca agatttactc
    17581 gaacaaaaaa aagcactggc gacacaaatg atcaaaaaag ccgaagcctc tccttgcttt
    17641 ttgttgtacc aaacgttccc tgggattggc gcactcacag ccgctttatt gctcggtgaa
    17701 ttaggagata tcacacgatt taaaacccat aaacaattga atgcctttgt cggaatcgac
    17761 atccgacggt atcattcagg taagtatact ggccaagacc gcatcaataa gcgtggtaat
    17821 cccaaagcac ggaaaattat tttcttcacc attcgaaata tgattcgcca gcaacgcgca
    17881 gctcctaatc atattgtcga ttattactac aaattaaaaa agcaacctat ccctaagaaa
    17941 gataaggttg ccacagttgc ttgtatgaac aagcttctga aatgtatgca cgctatggtt
    18001 agggcacata cagagtacga ctatgcgtac gcggtctcag tggaccatta actaaaccac
    18061 ttacagtata ccacttcttt gcccagatcg aaaatttttt acgaacgggg ctttatttag
    18121 tgtgccttga ttttgtcgct ttcgtttacg agctattttt cttgcacctt ttacttgact
    18181 aatcgtagga aagaggctgt gacaaaattc ttgtcgcagc tccctatttc ttgatgcagg
    18241 acagcttttt cacaacctct ccactaaacg gtgttcaatc agctgacctt cggtattagc
    18301 tcaaaaaacg gcaaaatatg aggagctatt ttcccgtttt tttctctaat actcaggtca
    18361 aaccgctgat ttcacaacct ctgattcacg gtgttccaga agtagcttct tcggaaataa
    18421 gccgaaattt tacaaaaact tgagaagcac ttttcgtaaa tttcttctta tttcttgaag
    18481 ctgaccactt ctgtcacaac ttctgttttt cattgtccat aggtttgata ttgtccttct
    18541 gatgactcat tcgtaatgat tgaggcttga ttttcttcat ttcccgttat cgtttgtcca
    18601 ctgccagaat ctgaagggct tagttctgtc gactcgtttt tttgagaaga agcagttgtt
    18661 ttcttgactt gttcgttggt ctttttccat tccgctagtc gatcggccac cacttttttg
    18721 tccacttttt cagaataagc tgtaaccact tttccatttt tcaaacgtgc taccttgtcg
    18781 ccatcatgag tcaaaagcaa aacgaccaaa tgtttgtctt tgaattgata ttgtctgaga
    18841 gaaatcgcac taagtacgtc atcgatattg ataaattgat tttcttcttt tccttttggc
    18901 aaatagatgt ccaattcacc tttttcgtaa ttggcagtta atttatattc ctcccaccaa
    18961 taagttgaga attctttttt gaaattatct actgattctt tgcttgctac gattttttct
    19021 gtttctttct gagcaagatc tgctgtcatt tttcctgaaa atgctgcgga taaaataagc
    19081 agtaagaaaa aaccgaataa ccaaaagaga agaaacggtc tttttttcct catgccttcc
    19141 tccactccta tttaattttg actattctat tatatacaaa aaatgaaata gaaagacaaa
    19201 aaagttgaaa aagatcgtaa ataattgaac ttctttttcc tgaagcggta agaacgggaa
    19261 ttttcataca ttctctcttg gctattcttt aagcttcaaa tccatttctg tctgatacat
    19321 ctttgaacca atgacccgat ttcttgatcg ttctttttcc atcttgtgct aaatctactg
    19381 aaataaaacc gtaacgattc ttataggcat tggtccacga ccagttatcc atgcatgtcc
    19441 acatatgata acctaaacaa ttggcacctt cttgaatggc ttgatggaca tatttcagat
    19501 gagattgaac aaattcgatc cgataatcat cgtggatcat accatcttcc ttgataaatc
    19561 gtgcttcatt ttctacaccc atgccgtttt cagaaataaa acagtcgata ttcccataat
    19621 gatctcgtac attgatcaaa atatcataga ttcctttttc atagatctcc catccgcgat
    19681 aaggattcat tttacgacca ggcatttcat aattgtcgaa gtaatcctca ggcataggac
    19741 cgtttttagt atcgattggt gtttcttttg cttttattcg gcgtggttgg taataattaa
    19801 ttcccaaaag atctactgtg ttttctttaa tgatcgtcaa atcatctgga tgaatctccg
    19861 gcattagatc gagttctttt attctctcaa tcaattcact gggaaactcg ccttttacag
    19921 ctggatcaag gaatgaacga ttgaagaacg catctgctat tcgtgctgct ttaacatctt
    19981 cgggatttga ttcgtctctt ggataactcg gtgtcaaatt taagatgatt ccgatcacac
    20041 cgctttgatc catttcatgg taagctttga tggctaatgc tgaagccaac gcttcatgat
    20101 aaccaacttg gattgccttt ttcatatctg attcttccgg ataatgccat cgatacaaat
    20161 agcctgcctc gactggaacg attggttcat tatgggtaaa ccattttttt actttcgagc
    20221 caaacaattg aaaacaactt tttgcgtatt tttcataggc ttcaaccgtt tctcggttca
    20281 accatccgcc tttttcttgc agatacatcg gcatatcgaa atgatacaga ttaacaaagg
    20341 gttcgatacc gttttcaatc aactcatcaa aatagtgatg atagaaacga acggcttctt
    20401 cgttgattgt tccagattca tcaggtatca aacggctcca ttggatagag gttcgaaatg
    20461 aattatgccc agtctgtttc atcaactgta catcctcttg atacttcgta taaacttgcg
    20521 aggttttttc aggtcctact tgatcaaaga acttttgcgg ttcgtttttg taccaataat
    20581 caaaaatatt ctctcctttt ccatcgcctg aaattcttcc ttctgtttgt ggaccacttg
    20641 ctgcagagcc ccaccaaaaa ttttttggaa attgatatct tttcatcaaa gcacctcttt
    20701 ttctttttta gttgattcca tgtatgatat tttccatcac aatcactttt tcattttctt
    20761 cctcttcttt tttgattcgt ttcaacagct gttctgctgc tcgttccccg attttatctg
    20821 ttggtggcgt gatcgtgatt tttggaagga tactcagatc gtttttatct aaataatcat
    20881 agtgactgaa cgcaatatcc ttcccgattc tgacaccttc ttgattgaaa cattcgatgg
    20941 caccaatcga catgttgtaa ttgctgacaa aaatagcagt tgcccctttt tctaataatg
    21001 cttttgcccc gtaatagccg ctttttctag aatagtctcc tgtaaagatc tctaaatgga
    21061 tagacggatc cttttgtacc gcttctttgg ctccagctaa acgttctctt gctacgtaat
    21121 ctgtttgagc ggcagcaata aatcctattg ttttatgtcc ttgtccgcca tgatgagcaa
    21181 tcacttttgc tacactttct cgatcattca ctactattgt atcaatacct gctggcccac
    21241 ccggacaatt caatgcgaca actgggatac tcatttttgt cagcatttca aatccagacc
    21301 actcttcttc tgataagaag acgatcaatc catcgatcgc atgcttcatc aggtattcga
    21361 ttttttgttc gattttttct ggctgatcgg caaacccgct taataataag ctatacccat
    21421 tttcttcagc tactctttcg atccctgaaa caacctccga accaaatcgg ttagatatcc
    21481 cgtttatcaa cagtccaata gagaaacttc gattattctt caatccttta gcaatgatgt
    21541 tttctttata gtccaacgct gaaatcgctt ctgatatttt ttccatattc ttttccttca
    21601 gctgctgacc gtttaaatag cgggaaactg ttcccactga aacaccagcc atcttagcca
    21661 cgtcacggat cgatgccata cattttcact cctgccagtt acatttgatt tctttttctt
    21721 gttgttcgac catgatgacc gctttctttg cataatgccg cttgtcatca atatccatat
    21781 aactaaagtc ttcagaagtg agttcaaaaa caagcagtct ttcctcttga gaccgcaatt
    21841 catctttacg aaaagctttc aataatttcg ttctttggat gacaacgccg tcaattaatc
    21901 ggacatagac aagagtactt gttttacctg aaacacttcc tgtattttta actttaattg
    21961 atattattac tttgtcaata tccgttttga cttccatctg actgtattca aatgttgtat
    22021 agcttagacc atgtccaaaa ggataaaatg gacttccgct taaatcataa taattctctt
    22081 gtttagaagc agcacgctga tagtaataaa caggtagttg ccgactattt ctaggataag
    22141 acaaactcaa tcgtccgctt gggttatttc ttccagaaat catctgcgca atggcttttc
    22201 caccctcctg tccaggaaac catgctgttg caatggcatt cgtaaatgac tcgattttca
    22261 tcaaatcgta gggtcttcct tgcacaagta tcgtgatgat tggcttgcct agttgataca
    22321 attgttcaat cagctgttct tggtatccac ctaaagaaag agaagcaaca tctacatttt
    22381 ctccagaatc catgttgatc cctttcgatg aaactgcccc gttacgaaga aattccatat
    22441 caaagttccg cgcacttgat ccacctagaa ctgcaatgat cacatctgag ttttgagcaa
    22501 gggtcaccgc ttctaataat ttttcttcct gatcctcctg atttcttact tcacatcctt
    22561 gtgcataaga aacctttgat tggggaaact ctgtttttat ctcttgtact atcttttttt
    22621 tcaaacggtc ttctgtttgt ggtgcagagt agtctcccaa caagtggtaa acatcgtgag
    22681 catttggacc tataacagca atgtttttta cttgttgatc caacggcaat ataccgtcat
    22741 tttttagtaa agtgatactt tcttttgctg tctctaaatt taagtgctct gattcctcca
    22801 tgagatgatc ccaatactcc tttggatcgt ttgcaaatgg ctgttcaaat aaacctaata
    22861 aaaatttgac agataaaact cggcgaactg cttgatctag tagcttttca tctacgactt
    22921 gattatcaat ggcttctgca atcttggtat aggtctcatc ccacaaactg agatcgattc
    22981 ctgccgctaa tgcatatgct gcagcagtct ttttatctgt aaaaacatcg gataaacgat
    23041 ccaaagccac accgtctgcc ataacgattc cttgataacc gatctctttt cttaatgttt
    23101 gctcaaaaag cgctcgattg atatgacaag gtatcccgtc gatgtcatta taagcagcca
    23161 tgacacctac tgcatttctt gtactcttca ataatggtcc gtaaatatcg gagaactctc
    23221 tgcgtccaat cgttactgtg cctgaattat gaccgcctaa agcttctcct tgtgcgatac
    23281 agtgctttaa cacaacaccg atctgatcct tcttcttatt gatttcctct actgtctgat
    23341 caagaaaaga aactttttct gaaataatct ctccttggaa ccctcgaacg accgcttcag
    23401 aaaaacgagc tgagagaatc ggatcttccc caaagcactc ttctgtcctt ccccaacgag
    23461 gatcttttgc cagatccaaa gtcgacacca gcgctaaatg aacaccttta gcagccagtt
    23521 cttttgccat caatcgactc gattgttcta ataatgctac attgaaagta tttccacggc
    23581 cgatattcgt tggataagaa atcccatcca agccttgatg accatgtgga cattcttcta
    23641 caagcaaaac tggaattttc catcgcgaat gctgtagtac atattcttgg actaggttag
    23701 ccaccttcca gctttctttt gcaggaatcc cattcttata atctgtttta gaccacggat
    23761 cagagcgaaa cagaccgtat aaggcaccca gaccgcctcc ccatttaaca tgttctttta
    23821 acttttctgt taattcgatc ttgtctccgt ttttttcata acattcccaa ccatacaaat
    23881 gctggtttac ctgtcctact ttttcagcaa tattcatcct cgaaagtaaa tcttctactc
    23941 tttggcggat ggttaacttt gggttttgat acgggtaatc gtacacctta atatcctcct
    24001 tctgccaatt ttgccttatt agcggctaat acaaatggca ggaataaaaa tacatctaga
    24061 atgataagta ttactgttaa gactgctgct cgtatatcgc ctcctgtagc aataaaagat
    24121 tgtataaccg gtggagtgat ccaaggtgca gcagcaactg ttttactgat gattccaaga
    24181 cttgtggctg cgtaggcaat aaccaagtta atagatggta taatagcgca tggaatcata
    24241 tatattggat tcatgactac tggtaatcca aacataattg gttccgtaat attaaataaa
    24301 caagtagtca gaccaaattt agcgattgat cgctgttctt gacgtttaga agcccacaag
    24361 atagcaataa ctaacggtaa aatacacccg ccacctccga tcaatccaaa tgcattggta
    24421 aagggctccg taataatatt aggaatttct tttcctactg aaaaagcatc catgttttcc
    24481 tgaatagact gtaacaaagg tgcttgccgt ataggagcca aaatactcga accatgcata
    24541 ccaaacaccc ataagaaatc agagaaaaac tgtaaaaata agatgcctgg gacggataaa
    24601 acaaaagctt tcaatggtgt ctgaatcgct aattcaatta attgcggaac attcaaacca
    24661 gtcgtatggt ttaaagtaaa aacgacaact gaaaaaaata aaatgacaac tatttctggt
    24721 actaaagaat taaatgacgc agctactgcc ggtggaactc cttctggcat cctgatttta
    24781 aaactcttca ttttcgctaa tttgataaat aattctgtac tgacaagggc agcaatcatc
    24841 gccgttgcta agccagacga agaagtcaga ctttgtaaga aaacccctgt agcttctgct
    24901 gtatttccgt ccatcaaagt gactgtactt gataaagaca tcaaaataaa cattacggct
    24961 gtacttagaa ttcctgcatg aacaggacta aatgatggat catcaatcac gcctgtttgg
    25021 atatagtggg ttgccaaatt ccagccgata ttgtaacaca cgataatagc taaaatactc
    25081 atcgtaccat tattcacgat tgaagcaagt tcagccaatg gacttagatc aagtattctt
    25141 tggactgttg catttgaaaa aatcagagca ttcagcaaaa ccatgacaga agcagccatt
    25201 actaacggca tagtaacgat aaaactgtct cgaatggcca gcaagtgtcg ctggctactc
    25261 atcttagata cgattggtag cattttttca gatagtctgt ctacgacttt cattatttca
    25321 tctcctagat tctaaaattg aaccggttca tttttaatat agcacttttt ttaaagattg
    25381 caacacattt ttttaaaacg ctttctaaag gtggtggatt ttttatgact tttgaaaaat
    25441 tattctattt aatggtattt tgtgaatttt ttctcttcca tccgtttgaa gttttacttt
    25501 ccaatggtat aatttcttta tcctatcaaa tgggggaagt attttgaaga aattaatcgt
    25561 actatttatt ttgtctgcgg gacttttgac attgactggc tgtgatttgt ttggcggctc
    25621 tgaccaatcc tcggacgaaa caaccgattc ttctgtctta aaagcggaaa aagcagatat
    25681 caccaacagt gtatatacgc taaaagtacg taaagatatg gatttatccg cgggacagtt
    25741 gacatttaca gataaaaaaa tggaatggac acgcacgtat cagacacaag gagctgattc
    25801 ttctgaatca tctgaagaaa aaacagtgct gacagatatc aagattacta ccaaagatga
    25861 cgaatatatt atttcaggaa aagaaaataa aaaagaagca acagtcacat ttaccaaaat
    25921 cggtaattac cgtttgaagg ataaggatgg caacatctac agtctatagt gccatctgtt
    25981 ggatcttctt ttgtttctcc ccttcttttc tgctatagta aagaagcaag atgactgtaa
    26041 aggagacgaa atggatgatc gatagtcctg attattgtac atgtaataac aacaaaaata
    26101 tccatacaga atttaatgat caaggaagtt tgagtatatg cgacaattgc cacaaactag
    26161 tcaaagattc ttttaaatcg tttgaatctt atcctgtcaa aaattatgta aaaagcaaaa
    26221 gaagataagc aaaatcaagc aaaactcact atggctgatt tttatgaact tcaaactcag
    26281 cttcttcctt tgatcaagct tcaaaaactg gaatcagtaa aacaaacagt ggatcatttc
    26341 agttcacaaa tcaaagagta acgttttatt tatttcacat ctttgaatat aaaataacca
    26401 aacgatagaa agacttattc tacatcacgt agtaattttt ctttcccgtc gtttggtttt
    26461 ttacttttag cctaattctt gacctttcgc atgctgatta tctgtaaaat attttccagc
    26521 aggctttcct tccaattcag ctaatttttc ttggatctga cgaatcacct tttttgcatc
    26581 ttctggattt tctacaaaat cgaatgtatc cccatccact tgtattttag gacatacatc
    26641 aaaatcctca taccattgat tatagcgggt gttcagtgtt tcgtaatagg tgtataacgt
    26701 ttcatcgctt tctagctgtt catagtcacg cccgcgtttt ttgatacgtt ctagcatcgt
    26761 gtccaaagaa actttgatat ggaccatcaa atcaggacgt ttttttggag caacatcatc
    26821 taactcgttt agcatcgtat ctaataagct atcgtactgc tgtacttcga tatccgttac
    26881 ccgacctaaa tcagcattta gatggaacaa cagactatct tcgtagatgg agcggtctag
    26941 cacattgtca tcgttcacta aagcattttt cattgctaaa aatcgcttat tcaaaaagaa
    27001 gatttgcaat aaaaatgcgt actgctcagg atttgaataa aaaagcggca atacttcgtt
    27061 atcttcgaca ttttcataaa atggtttaga atcaatctct tttgcaagca tatccgtcaa
    27121 actgctctta cctgcaccaa tcgttcctga aatcaaaatc actgacatac aacccttcct
    27181 cttctataag tactcgccta cgtaagtttc aacaggcaat ttttcaaaat catattcacg
    27241 tacagcttgg tctgatttta aatacgtgtg aagactttgt gtaatcatca agttcgtagg
    27301 taccgtctct tcttgtacga tgatcatcgg ttttttcaac atagttgcca cacctaattc
    27361 gtaagccgtt cctggatcga ttgtttgatg ctcaaagtca aggatagcca aaacaaattc
    27421 tgcatttgtc aactcttcca tatctttttc gtatacttcc tgcgcccatc cagcgctgaa
    27481 tagttcataa ttgctttctt ggtgatgacg tggagagtag aaacttgtga ttgttttgtt
    27541 ttcctccaac gctttttcga tacgacttac tcgatcgatt tgctcctctg aaaaaaatgg
    27601 tcctgctaaa tatatttgac tcattatttt gcctccttca ataataactg atttttaata
    27661 gatactgctc gtaattcatt gaccagttcc aatggattgt tggaaaagat cacttgatcc
    27721 aaccattttt tatccataaa atcctctttt gcaaaagttt ctaataattg ttgcaagccg
    27781 ctgtaataac cgccaatatt caatattccg attggcttat caggtgtcag gccgatcgct
    27841 atccaagaaa gaagttgcga aaattcctcc aatgtaccta gacctccagg gaaaacgagg
    27901 acagcatctc ctttttcaaa taataatacc tttctttcat caattgtctc tgtaggaata
    27961 aatgtagtaa catctttccc aggcagctca gtttcaaaaa ggccatttgg atagatacct
    28021 gtgacttttc cgttattttc cagtactgtt ttactaatca ctcccataat cccggagtat
    28081 gaaccaccgt aaaccagttc gatttcttct tgtgccatat attttcctaa cgtttgcgca
    28141 ataaatttgt aactttcttt attgccaaaa cgggaaccgc aaaaaacagt aagtttcata
    28201 aaaaaacact ccctaactca aaagtctatc ttcattctac acgtgttttt ttaaaataaa
    28261 agactagatt ttgtagtttt ttagtggtat atagatcaaa caccactata tatagtattt
    28321 gcaaaaaaat aatttttcag ctattttaca gtaaccaaaa aaataaataa ctagtaaata
    28381 taaaattgaa acgacttact tatagaagaa attgggattt cgtcctgcat gtcaataaca
    28441 cgcattattt aaatagtcac acacttgttt ctcctattaa tgaaaaatcc attttattct
    28501 cgtgttatgt ttcaccaacg cctgatgttt ctctaaataa aaaagccgac gaaccaaata
    28561 atcgaaattt gatcattttg gttcatcagc ttgttcccaa acctttgtat taacgataaa
    28621 tagctggatt cgtaaaacta tcacttggat catcactgct ggaagaaaat tcggtgataa
    28681 ccgcgcctgt tgcacctgcc tggaaccagt gtttaatatt ttgttcgatc gtgtactgat
    28741 ctccagcgtg aagtatgatt tctttttttg ccgtatagta acgttcttgc ccctttggaa
    28801 taatacagga aagttttttt gtttcttttc cttcgacata aagatatact aagccttttc
    28861 gacaacgaaa ggtttccttt tttccttcat tctcatctcg aggtgggtgc cgatgctctg
    28921 gacaagtttg ataaggcaat aataccagtt ctttcccaca gtatcgctca ttattttcat
    28981 aaacaaataa attcaatcct tcagtctcga ttgctcctaa tccaaaatct gtatattcga
    29041 ttctttctaa ttctgtttct ttcaatacta ctcctgaatc taggaacatt tgttgtactc
    29101 ttttcttgta atccattctg ttcctcctaa ctgattgtca agtcatccca agatatctct
    29161 tcttcctctt tttcttcttg gatgacttct gcatttgggt caactggttt tttcaataat
    29221 aaaatagtta ttcccgtagc aaaagaacca gctaacaatg caccaatgaa tgcccatggt
    29281 ctggtcatcg tagcgactgc aaacattccc gatgccggaa tacggctatc tgcgccccaa
    29341 aacatgctca atccaccgcc gactgcccca ccgactcccg ttgctatgac attgcgtgcc
    29401 aagtcgttca atacgatagg gatacatccc tctgtgatct gacagatccc cataggaaaa
    29461 gcggtcttca atgtatcgat ttcttgacga ttgaatatat ttttacgaat gattttccct
    29521 aagaaataag cgatcgtcaa gccgaatggg gttaccatcg aagctaaaat caatacagtg
    29581 attggttcat agattccttc tgaaaatcgt gccacacccc cttatccaga ataaatggta
    29641 atccagaaac agccgggtat ctgcggtaat aagcctaacc aaacgaaaaa tatgagaagc
    29701 taatatgaga agctattttt gtttggctgt tcttattact tgatgcaggg cagctttttc
    29761 acaacctctt tttttattta tgtttcataa tcaatatggt aaggatgcag ctccgtatgg
    29821 tagaccgcct ccgaatattc aatcatttcc ccaatttgcg tagacgattt ccgtatccgt
    29881 ttgatgccga tctgtgcgtc tgttttcaga atttcttgtt cttcttctgt taaaaatact
    29941 gccgagaaat catcagaaaa tgagtcaata gtatatcctt ttttcgtcag caaacgatac
    30001 aaagattctt tgccaaattc ttctctgctg aattgcatca tttcttgtgg aagataatga
    30061 acaaaataaa tataaggctg attgttcaaa taatataagc gcttaaaaca aacagcttgc
    30121 tcaccgaaaa aggaaaacaa aggatcttcc ttctttatat ggaccaattg caaatccagc
    30181 gtttctttcc ggacttcata tcgggagttc tccaagattt gcgtaaagct tgccgctttg
    30241 gataatcgat tgtaaggacg attgcttaga accgtcgtcc cacgaccact tttctttgcg
    30301 acatattcat ctgatgccag cagttcgatt gcctttcgga cagtgatttt gctgacttga
    30361 aacagttctt ctaattcact ttctgtcggt aacatcgaac cgactggata ttttcctgag
    30421 aaaatatctt ctttaatttt atctgcgact tccaagtata gtacacgtgt ccttttcata
    30481 acgtcaccct ttgctttact attactaaaa gaatagcaca taaaaaataa aaaaagaaca
    30541 caaatcacat gtgagaagtg tgttcttttt cattcagctg ctttaattac aagtttacgc
    30601 catttgaggc aattacttct ttataccaat caaaagattt tttctttgat cgattcagtg
    30661 tcccatttcc ttcatcgtct agatccacat agataaagcc gtaacgttta ctcatttctc
    30721 ctgttgatgc actgacaaga tccatacatc cccaaggcgt atagcccatc aaatctacac
    30781 cgtctgctac tgcattagcc atcgcttcga tatgacgacg taaataatcg atccgataat
    30841 catcaataat cgtgccatcc tcttccatat gatcaatggc tccaagaccg ttttcaacaa
    30901 tgaataatgg tttctggtaa cgattgtata attcattcaa tgcgattctc agcccttctg
    30961 gatcgatctg ccagccccat tcacttgcct caagaaacgg attttttact cctcccagca
    31021 agttcccttc tgaagttgct gcttgtggat cagtctcatt gattgccgta gacatataat
    31081 aactaaatcc gatgtagtcc actggatatt cttttagcaa ctggaagtct ttttccgttt
    31141 gctctaattt ttccggatcg actccatatt ttttatgggt acgcgctgtg tatgcagggt
    31201 attctccacg tacttggaca tccgtgcaga aaaaattgaa ttcttggttt tggatcattg
    31261 ttgctgcctg attgactgga ttggcatcga tgctgtaagt tgttgcatag ataatcatgc
    31321 agccgatttg gatatccgaa cgcaattcgt ggccgatttt tacggctttg ctacttgcaa
    31381 cgaattgatt atgccatgct tggaaaatat tttggtactc tcctgctcca ttgcttttta
    31441 ctaatccttg acttaaagct ggaaagtgaa aggccgaatt gatttcattg aaagtcatcc
    31501 aatatttgac tttactgctg tatctctcta atactgtctg agcaaatcgt tcatagaagt
    31561 cgatcagttt tcgatttttc cagccaccgt attcttttgc caaatgaaga ggcatttcgt
    31621 aatgagaaat cgtgatcaca ggttcaatat catattttaa gcactcatca atcagctgat
    31681 cataaaattc taatcccgct tcgttaggag taccttcgtc tccatttgga aaaattcgtg
    31741 tccaggcaat cgaaaaacga taacatttga atcccatttc cgcaaacaag gcaagatctt
    31801 ctttgaaacg atcgtaatga tcaattcctc gatgattcgg gtaaatatat ttttcttgat
    31861 caatcgtcca gtcaaattct tcactgccga taatcgcaaa gcgctgctta cctccaggca
    31921 tagcatctgc aacggatagc ccttttccgt ctcgatcata agccccttct aattgattgg
    31981 cagcagttgc tccaccccat aaaaattttt ctgaaaaacg tgattgcatc ctgatttcct
    32041 ccttatttta caattgccag aacatcttcg tttgcaataa tctctttttg gtctaattca
    32101 agtatatcgg caaatgcatc agtattcgta atgacaactg gagtgattac gctatagcca
    32161 gctgctctaa ttgcttcgat gtcaaaacga atcaataaat ctcctgcttt gacgagatcc
    32221 ccttgtttga ctaagatctc aaagccattc ccatttaatt ctactgtatc catcccgata
    32281 tgaatcaaaa tctccgctcc gtcagtcgtt gtgatcccta cagcatgtcc agttggaaaa
    32341 atagtcgtga tctctccgtc tgcaggtgca actagttctc ctacttctgg atcaatagcg
    32401 ataccttttc ccatcacgcc agaagaaaaa acttggtctg gtacttcact caatggcagt
    32461 actttacccg ttaaaggact ttttaaggta tgccgtgctg aagtaatcgg ttctcctgca
    32521 ttcgcatgtt tgttttctaa ttgattgtct tgtgtttgtt cgtcaaatcc taaaatcaat
    32581 gtacctacga atgcaagaac aaaagcaatt cctgtagcta ttactgctac tgtcacattt
    32641 gactctacac catccaccgt attaatgaaa gtcggaatcg tcaataagct gaccaaactg
    32701 ctggagaaag cttttactcc ggaaaatccg ataatagctc caccaatacc tccagaaata
    32761 caagcggcaa tgaatggttt cttcaaaggt aatgtcacac catacacagt tggttcagtg
    32821 attccaaata acgaagtcac tgtagaagaa atccctaatg ctcgaagttt cgtatctttt
    32881 gttcgcaatg ctacagctaa agcagcccca ccttgtgcca agatggctgg aagaagcatt
    32941 ggcatcaata catcaaaccc atattgttcg atattcaaaa acatgatcgg tacgaatccc
    33001 caatgcatgc cgaacatcac gaatacttgc cataaacttc ccatgattag acctgctacg
    33061 attggactaa atccataaat cgagttgaag agaccgccta aagcattacc ggcaaccgtc
    33121 ccgattggtc ctaaagcaag aaatgttaat ggaaccatca ctactaaaac gacaagcgga
    33181 actaaaatca tttgcagaaa ttgcggaatc acttttttta cgaatggctc taatttactt
    33241 tgaacccata cggctagaat gattggtatg acagaagaag tataaccaga tggtgaaaga
    33301 atcactggga tcccaaagaa actgatagct tctcccgcac cagcaactgc tgtgatagct
    33361 ggatacacaa gtgccatagc aattgctact gctaaaaact ggttggcttt gaacttttta
    33421 gctgctgtaa aagcaagcat gacaggtaag aaggtaaaga caccatctgc tgcagcaaac
    33481 aatatttgat aagtaccgct atcagctgtc agccagccca tcaccgttgc caatgataag
    33541 aatcctttca ataccccggc ggctgccatc gcccctagaa aaggtgtaaa tatactagag
    33601 ataatatcga tcagacgatt caaaatattt ttggactctt cttttccatc ctccgaaact
    33661 gcaagacctt cctcatccac gatcgattga taaacatctg ctacattact gccaatcact
    33721 acttgatact gaccgccact ttgtacgact tggatcacgt ccgggtcttc ttttaacttt
    33781 tgcgtatctg ctaaagattc atcttttaat ctgaatcgca accgcgtagc acaatgtact
    33841 aaacttttga cattcttttg tcctcctacc gcttcccata cacgacgacc aactgcttga
    33901 ttatccattg ttttttcctt ctttcttttt tatttaaaaa ataaaaaccc agattgaaaa
    33961 aaacagtcac aaacattcgt gaacctattt tttcaatcca ggttttgcct gccatacagt
    34021 aacaatcctt atgattggat gctttttttc tcacttacta ctctttgaat atgaatcgtt
    34081 aaataaatct gctctgcgat ccccatttcc tgtttttgcg tttgtcgaag atactcattg
    34141 atccgttcga cacaatgaaa cgcttttgga tactttcctt gaactaatgc gtagagaaat
    34201 tcatcttgct cgtcagatcc ttcccctttg agataacgct gggcaaaaaa ctgcagatga
    34261 gtaatgatac gctgataatt caaagattct tcatcgaaca cgatgccgaa gaatttactg
    34321 atgatactca gtatatctct taccattgtg gtagcggtca tcgtcacttg catgtcttcg
    34381 tttccttgtt ggctattcac aaaatgaaag gcaataaaac cggcttcatc ctcagaaagt
    34441 tccacacccg tcttttgttt cacgaacaaa agagcctctt tcgctgcttg atattctttc
    34501 ggataaaatt tttttgtttc aaataataat ggattaggca gttggatatt ttcttttgct
    34561 cgtgtgatcg cataatgaag atgatcggtc aatgtcagat aaatattagg cgaaaattca
    34621 acttttagtt tatcttcagc cagttcgact acttgtttga cccattcgat ctcctctgcc
    34681 ggaatatcag ccatcagctg ctggaattgg tttttagcaa aagaatctct taaaacaaat
    34741 tctttttgga ccgcttctgg cgaaatctcg tccccaatct ttttttgaaa agctagtcct
    34801 tttcccatac aaacgacttc ttccccgaat ccatttttcg aaatgacgac attgttattc
    34861 aaaattttat caattttcat tttgcttcgc cctctcaaga aaaataaaaa aacctagaat
    34921 tcgcctactg tttcttagct gaattctggt tttgcctgcc gtacagtaac aatccatttc
    34981 ttattcaaaa tcatcatacc atcgaatttt tatttatgca agccttttca tataaaaaat
    35041 aaaaaaactg gaacacaagt ctatctagaa aacaaccaaa tcagagatgc acctctttgc
    35101 gtttctcttt ttttcagcaa attggttttt ccttatcgat tcatatcctt tggctgtttt
    35161 taatctgtaa cgacttatgt cccagtatcc taaaatgttt ttgtctctag attgaaagca
    35221 gatctcatat catctgtcgc atcttttatc gatgtaacac tttccagttc tccttgtaca
    35281 actagtcgcg tgacggcgtc catgtcgcca caagtaatta gtgtgacaat ttcttttccc
    35341 tcgacttcat tcagtaattg ggtatctgta ggagcaactt tttctttaaa aaatgttttg
    35401 tatgtataca cattttctaa atctgtcaaa tagatcttgg cacccatctc taaattttct
    35461 agcggtgtaa acagcaattc agggttcgtt gcacgatggc ttgctaaggc ataatttcct
    35521 tttcccattt cttgatctgg agataatgtt cctgctccgt aaagtagtga cgtattatcc
    35581 aagcctttga aaaccggtaa attgattgaa actgaaggga ctgcgatact agcaatcact
    35641 ggtagatctg ttcctctcat ttgcgacctc atgacccctt ctgtcgtcat tggttctacc
    35701 gcatcaaagt caaaactgac atctttatcg ttattctttt tcagatcttc ttttgttaca
    35761 ttcgctatag catatttatc tcctgtctct ctgacaaaat aatccttgat ctgttcattg
    35821 aaaatcaaag ctagtcctac taataacaag aggaacaaga ggatattgac tagccatctt
    35881 tttgcttttc cttttttttt cattttctct ccctccagac tcattttact gtttttcttt
    35941 tgctctttcc aacagccgaa tgacacattt ctacaactct gtgtattttt ttcgtacacc
    36001 tgcgcttttt tctacaggat atttcaaatt atttttgatc aatttttctt caatgatctc
    36061 atctagatcc aggtgaaggt tgtctgccat catgtatgaa tagatcaata catctgccag
    36121 ttcttctttg attcgttcta attgtgtttc ttttacttct tccggcgttt tccattgaaa
    36181 aagttctaac aattcagatg cttccaaaga aatagagata gccaaatctt tttcgttatg
    36241 aaattgtcgc caattacgtt catctctaaa ctggttgact ttttccatac tattcatttg
    36301 cttgccttct tccaatcact tcttacttga ttaatcatac cattttttta cataaaaaat
    36361 agatgatgct tttaaaaaaa ctcctgtttt cttttattta aaataaatgt catagaaaat
    36421 caataattta ggtagccacc atgtgtatgc agtagcgtgt cttcttccct tgaggctttt
    36481 tgtttttcga tacgttcttc ttcccaaaca gacaacgcta ctagaatctc tcttaatgat
    36541 tttccttctt cggtgagcga gtactctact ttaggcggga cttggttata ggcttttcga
    36601 tggatacttt catcttgttc taactctctt aactgctggg tcagcatttt tttgtgatcg
    36661 tagggatttg cttgcttctc cacgcctaaa ataagcgata ttgctcttcc ctttatctgg
    36721 tgtaagcaaa gcagttagca ttttgacgat cgtgggttcc gcttttcact tcttatctac
    36781 ttagtatcat gaagtatact ttcgtactaa tactaagtag cggaagtgtc aactactttg
    36841 tttaactaca tagcaaaaga aacagaaggg tgacaacggt catcacgctt ctgtttcttt
    36901 tgaatatgct gtgttgttgt ctctatctcc gtgtagactt tcctcaaact tatcctttga
    36961 cagtaagtca gtaggctgat ttgctctttc tagatgccag atacatttct acaaaaaaat
    37021 agtgattctg ttttgaaaat tcgattttta gttctgcatt ttcgtaaaca tccaacattt
    37081 ttttcgcact gtatagacct aacccgccat tttttttaga ggtttttcct cttttgacca
    37141 tctccgatag cgtcaaattc gttggatttg ccttgttcgc tatactaaaa cgtgatccct
    37201 ctttttcttc cagcagattg atcgtaatcg gactctgatc ggctacacga tgatcgaatg
    37261 cgttagaaat aaaaatagat aaacagcgaa taaaaacaac cagatcaatc gggattgttt
    37321 tcacttcgac aggaataact acttggatcg ggattttctc cgattctgct ttttctagaa
    37381 attttaaaaa gacacccctg acagctgggg atacaatatt tgttagctgt tgtatttgcg
    37441 gtttttcaaa aatagctgca gaatgatctt ctaatatttc taaaaattcc aac
//
LOCUS       DAZUMK010000014        34719 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000014 DAZUMK010000000
VERSION     DAZUMK010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.014"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="ACG808_000711"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="ACG808_000711"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFS5046362.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(937..1518)
                     /locus_tag="ACG808_000712"
     CDS             complement(937..1518)
                     /locus_tag="ACG808_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5046363.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="ACG808_000713"
     CDS             complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="ACG808_000713"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFS5046364.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2653..3066
                     /gene="rpsL"
                     /locus_tag="ACG808_000714"
     CDS             2653..3066
                     /gene="rpsL"
                     /locus_tag="ACG808_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFS5046365.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3150..3620
                     /gene="rpsG"
                     /locus_tag="ACG808_000715"
     CDS             3150..3620
                     /gene="rpsG"
                     /locus_tag="ACG808_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFS5046366.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3714..5801
                     /gene="fusA"
                     /locus_tag="ACG808_000716"
     CDS             3714..5801
                     /gene="fusA"
                     /locus_tag="ACG808_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFS5046367.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5973..7160
                     /gene="tuf"
                     /locus_tag="ACG808_000717"
     CDS             5973..7160
                     /gene="tuf"
                     /locus_tag="ACG808_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFS5046368.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7567..7875
                     /gene="rpsJ"
                     /locus_tag="ACG808_000718"
     CDS             7567..7875
                     /gene="rpsJ"
                     /locus_tag="ACG808_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFS5046369.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7906..8535
                     /gene="rplC"
                     /locus_tag="ACG808_000719"
     CDS             7906..8535
                     /gene="rplC"
                     /locus_tag="ACG808_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFS5046370.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8563..9186
                     /gene="rplD"
                     /locus_tag="ACG808_000720"
     CDS             8563..9186
                     /gene="rplD"
                     /locus_tag="ACG808_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFS5046371.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9186..9476
                     /gene="rplW"
                     /locus_tag="ACG808_000721"
     CDS             9186..9476
                     /gene="rplW"
                     /locus_tag="ACG808_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFS5046372.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9515..10348
                     /gene="rplB"
                     /locus_tag="ACG808_000722"
     CDS             9515..10348
                     /gene="rplB"
                     /locus_tag="ACG808_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFS5046373.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10391..10669
                     /gene="rpsS"
                     /locus_tag="ACG808_000723"
     CDS             10391..10669
                     /gene="rpsS"
                     /locus_tag="ACG808_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFS5046374.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10692..11039
                     /gene="rplV"
                     /locus_tag="ACG808_000724"
     CDS             10692..11039
                     /gene="rplV"
                     /locus_tag="ACG808_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFS5046375.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            11053..11709
                     /gene="rpsC"
                     /locus_tag="ACG808_000725"
     CDS             11053..11709
                     /gene="rpsC"
                     /locus_tag="ACG808_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFS5046376.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11712..12146
                     /gene="rplP"
                     /locus_tag="ACG808_000726"
     CDS             11712..12146
                     /gene="rplP"
                     /locus_tag="ACG808_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFS5046377.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12136..12324
                     /gene="rpmC"
                     /locus_tag="ACG808_000727"
     CDS             12136..12324
                     /gene="rpmC"
                     /locus_tag="ACG808_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFS5046378.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12349..12615
                     /gene="rpsQ"
                     /locus_tag="ACG808_000728"
     CDS             12349..12615
                     /gene="rpsQ"
                     /locus_tag="ACG808_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFS5046379.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12673..13041
                     /gene="rplN"
                     /locus_tag="ACG808_000729"
     CDS             12673..13041
                     /gene="rplN"
                     /locus_tag="ACG808_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFS5046380.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13078..13386
                     /gene="rplX"
                     /locus_tag="ACG808_000730"
     CDS             13078..13386
                     /gene="rplX"
                     /locus_tag="ACG808_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFS5046381.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13414..13953
                     /gene="rplE"
                     /locus_tag="ACG808_000731"
     CDS             13414..13953
                     /gene="rplE"
                     /locus_tag="ACG808_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFS5046382.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13972..14157
                     /locus_tag="ACG808_000732"
     CDS             13972..14157
                     /locus_tag="ACG808_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFS5046383.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14193..14591
                     /gene="rpsH"
                     /locus_tag="ACG808_000733"
     CDS             14193..14591
                     /gene="rpsH"
                     /locus_tag="ACG808_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFS5046384.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14623..15159
                     /gene="rplF"
                     /locus_tag="ACG808_000734"
     CDS             14623..15159
                     /gene="rplF"
                     /locus_tag="ACG808_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFS5046385.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15320..15676
                     /gene="rplR"
                     /locus_tag="ACG808_000735"
     CDS             15320..15676
                     /gene="rplR"
                     /locus_tag="ACG808_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFS5046386.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15697..16197
                     /gene="rpsE"
                     /locus_tag="ACG808_000736"
     CDS             15697..16197
                     /gene="rpsE"
                     /locus_tag="ACG808_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFS5046387.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16212..16391
                     /gene="rpmD"
                     /locus_tag="ACG808_000737"
     CDS             16212..16391
                     /gene="rpmD"
                     /locus_tag="ACG808_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFS5046388.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16435..16875
                     /gene="rplO"
                     /locus_tag="ACG808_000738"
     CDS             16435..16875
                     /gene="rplO"
                     /locus_tag="ACG808_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFS5046389.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16875..18170
                     /gene="secY"
                     /locus_tag="ACG808_000739"
     CDS             16875..18170
                     /gene="secY"
                     /locus_tag="ACG808_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFS5046390.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18232..18879
                     /locus_tag="ACG808_000740"
     CDS             18232..18879
                     /locus_tag="ACG808_000740"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFS5046391.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19070..19288
                     /gene="infA"
                     /locus_tag="ACG808_000741"
     CDS             19070..19288
                     /gene="infA"
                     /locus_tag="ACG808_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFS5046392.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19321..19437
                     /gene="rpmJ"
                     /locus_tag="ACG808_000742"
     CDS             19321..19437
                     /gene="rpmJ"
                     /locus_tag="ACG808_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFS5046393.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19455..19820
                     /gene="rpsM"
                     /locus_tag="ACG808_000743"
     CDS             19455..19820
                     /gene="rpsM"
                     /locus_tag="ACG808_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFS5046394.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19848..20237
                     /gene="rpsK"
                     /locus_tag="ACG808_000744"
     CDS             19848..20237
                     /gene="rpsK"
                     /locus_tag="ACG808_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFS5046395.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20317..21255
                     /locus_tag="ACG808_000745"
     CDS             20317..21255
                     /locus_tag="ACG808_000745"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFS5046396.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21283..21663
                     /gene="rplQ"
                     /locus_tag="ACG808_000746"
     CDS             21283..21663
                     /gene="rplQ"
                     /locus_tag="ACG808_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFS5046397.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21935..22774
                     /locus_tag="ACG808_000747"
     CDS             21935..22774
                     /locus_tag="ACG808_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS5046398.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22750..23619
                     /locus_tag="ACG808_000748"
     CDS             22750..23619
                     /locus_tag="ACG808_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS5046399.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23609..24406
                     /locus_tag="ACG808_000749"
     CDS             23609..24406
                     /locus_tag="ACG808_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFS5046400.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            24609..25355
                     /gene="truA"
                     /locus_tag="ACG808_000750"
     CDS             24609..25355
                     /gene="truA"
                     /locus_tag="ACG808_000750"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFS5046401.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(25416..25622)
                     /locus_tag="ACG808_000751"
     CDS             complement(25416..25622)
                     /locus_tag="ACG808_000751"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046402.1"
                     /translation="MVTEKIGNKKMIHLSTPRPQVTIFIKDEKLFFEIALCDSELNDR
                     LTTSFLNGVPEVASSKISRNFAKF"
     gene            complement(25688..28357)
                     /locus_tag="ACG808_000752"
     CDS             complement(25688..28357)
                     /locus_tag="ACG808_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFS5046403.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(28595..28783)
                     /gene="rpmB"
                     /locus_tag="ACG808_000753"
     CDS             complement(28595..28783)
                     /gene="rpmB"
                     /locus_tag="ACG808_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFS5046404.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            29053..29415
                     /locus_tag="ACG808_000754"
     CDS             29053..29415
                     /locus_tag="ACG808_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS5046405.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            29467..31149
                     /locus_tag="ACG808_000755"
     CDS             29467..31149
                     /locus_tag="ACG808_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFS5046406.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            31266..33302
                     /gene="recG"
                     /locus_tag="ACG808_000756"
     CDS             31266..33302
                     /gene="recG"
                     /locus_tag="ACG808_000756"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFS5046407.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            33351..34352
                     /gene="plsX"
                     /locus_tag="ACG808_000757"
     CDS             33351..34352
                     /gene="plsX"
                     /locus_tag="ACG808_000757"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFS5046408.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            34474..34716
                     /gene="acpP"
                     /locus_tag="ACG808_000758"
     CDS             34474..34716
                     /gene="acpP"
                     /locus_tag="ACG808_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFS5046409.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT        11330 a         6380 c         7470 g         9539 t
ORIGIN      
        1 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
      961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
     1021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
     1081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
     1141 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
     1201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
     1261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
     1321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
     1381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
     1441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
     1501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
     1561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
     1621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
     1681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
     1741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
     1801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
     1861 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc
     1921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
     1981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
     2041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
     2101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
     2161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
     2221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
     2281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
     2341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
     2401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
     2461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
     2521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
     2581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
     2641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
     2701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
     2761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
     2821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
     2881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
     2941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
     3001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
     3061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
     3121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
     3181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
     3241 tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca
     3301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
     3361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
     3421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
     3481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
     3541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
     3601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
     3661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
     3721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
     3781 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
     3841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
     3901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
     3961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
     4021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
     4081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
     4141 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
     4201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
     4261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
     4321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
     4381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
     4441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
     4501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
     4561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
     4621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
     4681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
     4741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
     4801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
     4861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
     4921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
     4981 gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg
     5041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
     5101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
     5161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
     5221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
     5281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
     5341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
     5401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
     5461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
     5521 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
     5581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
     5641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
     5701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
     5761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttaaa accggatagt
     5821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
     5881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
     5941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
     6001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
     6061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
     6121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
     6181 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
     6241 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
     6301 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
     6361 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
     6421 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
     6481 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
     6541 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
     6601 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
     6661 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
     6721 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
     6781 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
     6841 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
     6901 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
     6961 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
     7021 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
     7081 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
     7141 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
     7201 taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga
     7261 tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt
     7321 ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga
     7381 gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc
     7441 agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga
     7501 acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg
     7561 aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta
     7621 gatcaatcag cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt
     7681 ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac tcataaatac
     7741 aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac
     7801 ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc
     7861 gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc
     7921 ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta
     7981 actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc
     8041 tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg
     8101 aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat
     8161 gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga
     8221 gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc
     8281 cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca
     8341 atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt
     8401 ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt
     8461 atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct
     8521 gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta
     8581 ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt
     8641 gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa
     8701 ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt
     8761 caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt
     8821 ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt
     8881 cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc
     8941 gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta
     9001 tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca
     9061 gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt
     9121 gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt
     9181 gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg
     9241 ccatggatga caagaaatac acttttgaag tggacactcg cgcaaacaaa actttagtaa
     9301 aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac
     9361 gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga
     9421 tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac
     9481 taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac
     9541 ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc
     9601 tgaaaaatca ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca acggccgcat
     9661 cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg
     9721 taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc
     9781 taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg
     9841 actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc
     9901 tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg
     9961 taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg
    10021 caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg
    10081 tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg
    10141 acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga
    10201 tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc
    10261 ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact
    10321 tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag
    10381 gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa
    10441 aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg
    10501 ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca
    10561 tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac
    10621 aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag
    10681 gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca
    10741 cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg
    10801 caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga
    10861 tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa
    10921 gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat
    10981 cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag
    11041 gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc
    11101 gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta
    11161 agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc
    11221 gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt
    11281 ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa
    11341 agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt
    11401 gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc
    11461 atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta
    11521 aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact
    11581 ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga
    11641 gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga
    11701 gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa
    11761 tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg
    11821 ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt
    11881 acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca
    11941 aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag
    12001 taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag
    12061 cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa
    12121 tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt
    12181 gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt
    12241 caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact
    12301 gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga
    12361 agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt
    12421 accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct
    12481 aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt
    12541 atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa
    12601 gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg
    12661 aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca
    12721 cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt
    12781 gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac
    12841 gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat
    12901 attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca
    12961 cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca
    13021 ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg
    13081 tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc
    13141 gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt
    13201 aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg
    13261 ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc
    13321 tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat
    13381 aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt
    13441 aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct
    13501 aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac
    13561 ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa
    13621 gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt
    13681 acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca
    13741 cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact
    13801 ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta
    13861 cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg
    13921 ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa
    13981 aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt
    14041 tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc
    14101 ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa
    14161 gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt
    14221 ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat
    14281 caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg
    14341 aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg
    14401 gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca
    14461 aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg
    14521 aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt
    14581 acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat
    14641 aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt
    14701 aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa
    14761 ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga
    14821 acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg
    14881 ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac
    14941 gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca
    15001 gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca
    15061 aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc
    15121 gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag
    15181 aagccagcag caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta
    15241 cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa
    15301 aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag
    15361 acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt
    15421 tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc
    15481 aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc
    15541 tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtctttga
    15601 ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa
    15661 tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat
    15721 ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga
    15781 cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtagggttt
    15841 ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag
    15901 aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct
    15961 tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt
    16021 ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta
    16081 ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa
    16141 cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga
    16201 ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc
    16261 aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta
    16321 agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg
    16381 aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa
    16441 cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt
    16501 acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca
    16561 ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca
    16621 aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg
    16681 aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg
    16741 aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact
    16801 gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca
    16861 atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat
    16921 cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg
    16981 taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt
    17041 tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac
    17101 catacattac tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg
    17161 ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc
    17221 tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg
    17281 ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc
    17341 taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg
    17401 gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca
    17461 gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta
    17521 tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc
    17581 aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa
    17641 gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt
    17701 cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa
    17761 actggtacaa agtgatgatg gagatcttta actataatac agcacctggt gctacgattt
    17821 atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga
    17881 aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg
    17941 ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc
    18001 ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa
    18061 tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac
    18121 agttagaagg cttaatgctg aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt
    18181 tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc
    18241 attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct
    18301 tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca
    18361 gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta
    18421 acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta
    18481 gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg
    18541 aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt
    18601 ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct
    18661 acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat
    18721 gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc
    18781 ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat
    18841 gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact
    18901 tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac
    18961 ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc
    19021 gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga
    19081 agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt
    19141 tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta
    19201 catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg
    19261 tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc
    19321 atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa
    19381 ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg
    19441 gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacgtgtag
    19501 tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg
    19561 atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta
    19621 tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga
    19681 acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc
    19741 ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta
    19801 tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa
    19861 aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt
    19921 ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt
    19981 catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa
    20041 tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa
    20101 cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt
    20161 tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa
    20221 aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac
    20281 aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga
    20341 atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga
    20401 gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt
    20461 gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt
    20521 gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat
    20581 ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc
    20641 gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc
    20701 tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa
    20761 gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac
    20821 acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat
    20881 tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg
    20941 agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa
    21001 gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa
    21061 atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga
    21121 atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac
    21181 ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta
    21241 cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta
    21301 ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc
    21361 aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa
    21421 atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta
    21481 cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta
    21541 caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc
    21601 attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc
    21661 taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga
    21721 agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa
    21781 gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga
    21841 aggcataaaa aaacatttta agcgagaaat aattgaaaat tttgtctttt tttgatagtg
    21901 ttagtaggaa ctttaggatg aagaaggagt aatcatggag ccaattattg aattagaaaa
    21961 gataaattat aaataccagc cagatgatct tcgccccgca ttgaaagatg tctcctttac
    22021 aattgataaa ggtgagtgga tcgctattat tggacacaat ggttcaggaa aatcgacact
    22081 tgccaaaacg atcaatggat tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca
    22141 aatattggat gaagaaaata tttggacgat ccgacagatg gttggaatga tttttcaaaa
    22201 tccagacaat cagtttgttg gttcgacagt agaagatgac gttgcttttg gtcttgaaaa
    22261 tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag
    22321 aatggctgag tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt
    22381 tgccattgcc ggcgtggttg ccttacgacc tgatatcatt attttagatg aagcaaccag
    22441 tatgctagat ccagaaggtc gtgaagaagt gatctctacg atcaagaaga tcaaagaaga
    22501 aagtcaattg accgttatat ctatcactca tgatattgat gaagcagcta atgccaatcg
    22561 gatactagtg atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc
    22621 tgctggtcct gaactgatcg acttaggtct agatctacca tttcctgaaa aactgaagag
    22681 cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag aaaggatggt
    22741 ggattggtta tggacatccg ttttgaacaa gtagacttca cttatcagcc gaatacacca
    22801 tttgaacaaa gagccttatt tgacatcaat atgacgatca aagaaaacag ttatactgca
    22861 ttagtagggc ataccggaag cgggaaatca accttacttc agcatttgaa tgcgcttgtt
    22921 aagccaacaa gcggtacagt ccatattgga gaacgagata ttcagccgga tacggataat
    22981 aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag
    23041 ctattcgaag aaacggtagc aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc
    23101 gaagaagaag cactagtcct agcaaaagaa acattagaac aagttgggct ggatgaaagc
    23161 tatttggaac gttcgccatt tgaactttca ggagggcaaa tgcgtagagt agcaatcgct
    23221 ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg aaccaacggc aggacttgat
    23281 ccccagggca gaaaagaaat gatggagatg ttttggcgtc ttcataagga acagcagatc
    23341 actattgtat tagttacgca tttaatggat gatgtagcca attttgcaga ttatgtctat
    23401 gtgttggaaa agggacgaat cgttaatagc ggggagccgc aagaggtctt tcaaaacatt
    23461 gaatggctaa aagaaaaaca actaggcgta cctactgcca ccgagtttgc agaagaatta
    23521 atggctaaag ggatgaactt tgctaccttg cctttgacag ctgaagaatt agcagacgct
    23581 atcgtccatt atgctggagg aaatgcccat gatgaataaa ttgatatttg gtcgttatat
    23641 gcctggagat tccttgatcc atcgattgga tccccgagct aaattattag ccagttttta
    23701 ttttatcgga atcatttttt tagcaaataa ttgggaaacg taccttttgt tggcattttt
    23761 cacattactt tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg
    23821 cccattgatc tggctaatcc tcttcactgt agctttgcag gtcttgttta caagtggcgg
    23881 agaaacttat tggagttggg gtgttttcca tatcactgaa tttggtgtga tgaacggcct
    23941 ctttattttc tgtcgattcg tgttgatcat ttttatgtcg actctactca cgcttactac
    24001 accgcctttg gaattatcgg atgcgattga gtacttgctt cgtccattga aagccgtacg
    24061 gttcccagtc cacgaaattt cattgatgct gtcgattgct cttcgttttg tgcctacatt
    24121 gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg attttggtga
    24181 aggaaaccta gttcagaaaa tgaaagcagt cattcctttg ctgattcctt tatttgtcag
    24241 cagcttcaac cgtgcggaag atcttgcgac tgctatggaa gcaagaggct acaaaggtgg
    24301 ggaaggaaga acgaaatatc gtgtattgca ttggcataat agagacacga tggttatcat
    24361 tgcttttgta ttattaactg tgctactgat ctttttaaga ggataaacgt tcagtgggcc
    24421 gctatctatt cttttcttgc tgatctgttt gcaagaaagg atgcaagcag tgtatgacaa
    24481 gaaatattag ggtctgtggc agactaaggt cacaggcctc tttcttttat ctgaaaagaa
    24541 agaacgaaaa aattttatag aaaaatcatt attccagata accttttagg aacaaaggag
    24601 agaaaaacat ggtacgttac aaagcaatca ttgcgtacga cggaacaaat tttaatggat
    24661 tccaaaaaca gccaaatggg cgtactgttc aagaagaagt agaaaaaaca ttacaaaaaa
    24721 tggcaaatgg aaaagaaatc acggtctttg gttcgggaag gacagatgcc ggagtacatg
    24781 cgatcgggca agtaatccat tttgattatc cagaggagcg tccgttagaa cgaatgcgtt
    24841 tcgcgctaga tacccagtca ccagaggata ttgctgtaag acaagtagaa attgtttcag
    24901 aagacttcca cgcaagatat ttggtcaaag aaaagacgta tcaatttcga gtggatatag
    24961 ggaaaccacg cagtcctttt cgacgccatt atgcaagtta ttttccttac ccacttgatt
    25021 tggaaaaaat tcagcgggcg cttcctgatt tattagggac tcacgatttc acctcttttt
    25081 gtgcatcagg aagctcgata gaggataaaa tacgtacgat ctacgaggct aagatggagg
    25141 taaatgaaac aaaagacgaa ttgttgttta catttagggg aaatggcttc ttgtataaga
    25201 tgatacggat cttagtagga acgcttttga aaattggcaa tggccgattg cctgaagata
    25261 gtatcccaga tattattgcg aaaaaagatc gtaatgcagc aggacctaca gcacaccctg
    25321 aaggacttta tttgtatgaa gtgaaatatg aataagaggt tatgatagaa gtggtcagct
    25381 tcaagaaata agaaggtatt tacgaaaatt acttctcaaa attttgcaaa atttcggctt
    25441 attttcgaag aagctacttc tggaacaccg tttaagaaag aggtcgtgag cctgtcattt
    25501 aactctgagt cacaaagagc aatttcaaaa aacagcttct catctttgat gaatatcgtg
    25561 acttgtggac gaggcgttga taagtgaatc atctttttgt tacctatttt ttctgtaacc
    25621 aaaaaacgat ggaacgttgt tttgattgcc cttggtgcgg acagtctgaa caggttccat
    25681 cgtttcttta ttttctacgt cttcttctgt gtgtgtttcg tggttgttct tctattgctg
    25741 gatcaatcgt gccataaaag gggcgattgc ggtcaaggta ccagacataa agaataaaca
    25801 atgcaataca taggacaaaa aggattgcac ctatcgtaaa tccttcaggc aagtaactga
    25861 aagtgacagt atgtttacct gctggaactt ttatcatcaa gaatgcgtct ttgaaattgc
    25921 tgaccttgac tggtttccca tcaattttag ctttccatcc tttgtcataa ggaatagttg
    25981 tcaccaacat ggaatcttca gcggtattta cagtcccaga agcttttctg ccagtggctt
    26041 ccatttcgac acctttttct ttgatctgat tcattgcggc ttgataggct acaacatcta
    26101 gaccaacgac tttaggctcc atgaaactta tttctttcgt cccataaaaa cttgcagtaa
    26161 atgtcactgt tgtctcttga ggataatagc caagatcata gtattgacca gaaatgttga
    26221 tttgcgtttt tcgcgatgta ccattgacag tgatcgtcgc actagaactt tctaattgcg
    26281 caaaattggt tgggaacaaa cttaagtaag cttgtgtatt tgcaggcaca tggacttccc
    26341 aagtgagctc ttttggctgg ttatctgctg tttctgagta agtgacgcca gtcgttgtct
    26401 ttgtgatcgt cgtattcaac tgattcttca aagtaggtgt atagaatgta aaatactgtt
    26461 catttagacc agacaatgta ttgatcaagt ttgtctgact ggtcaaatta tctgtcactg
    26521 gctgctcaac atcattaacg gacaacggtg cagtaaatgc tagtggcaat gcattgacat
    26581 tctgatacag tgtatagtcg ccgctttgac cagcttggcg gaagccatat ttactgaacg
    26641 tgctttcgtt tttagaaatg ttatatttga ttcctgtaaa cccatccatc agcaaagtat
    26701 tattgttata acggatattc aaatttgtcc ctcttgaacg gaaacctaat ttatccaggt
    26761 aagaggaaga attacgattt cttatagaag aaaacaaact gataccactg taaccatagt
    26821 tgatcgaatc gtttgcagat actggatcca gattttccaa gcggtaaaac gagtcggaat
    26881 ctttttttgt ttcatttaca agagctttca agtcaggata aggatcagta tataaactgc
    26941 gggaagcata attccaatca tcaagaatgc catggatcat actgttcgta ttgaccagtg
    27001 cttcagcaga catgatcaat aataacaata tactcagatg attgaatgaa agttttttat
    27061 gctgataaaa tcccacagca aaagcataaa tccctaagaa aaccactgtc aatacaaagg
    27121 aagtcatcgt cacataggtg tagcttcctt caggtttgat gccccatgcc accgcaaaga
    27181 gagcagctac gaccaaaatg attcctaaca acagcccacc atcgtcttgt tctatctttt
    27241 cccatccttt tcctgcaagc aggagcacta agaaagagaa cagataggca taacggaata
    27301 aaaacatatt cggtgcatgc ataccatgcc agaacaaatt caatggaaca agataaaaac
    27361 ttgcgataag tatggcgaaa agactgccgt acaaaaattt atttttcaac ggtactttcc
    27421 gactggcaaa ataaaacaga cagaggacta atggtagcag acctgcgtaa acgaacggaa
    27481 tcgatccgta ttttgtcgtg tcataaacac caatcatatt tttcatgatt agatccagat
    27541 aagaagtcgc ttctgttttc aacgtagtga tctgactcag tgcctcgcca tttgaccgaa
    27601 gatccagcac tgctggcaat acaatgatca ttgaagcgcc gcctgccagt aaagaagtga
    27661 taccataagg aacaatcgtt tttttatatt ctgaccagtt tgtcaataga cgggcgataa
    27721 aatataagaa agaaaatacg ccaatcataa aacccatata aaaactggat aaaaacaaga
    27781 gtaaataact gataaataaa agtttcggcc gtttaagatc cataacacga tgaatcccca
    27841 aaatcactaa tggaagataa acaaatgcat ccagccacat gatgatctcc gaatgtgcag
    27901 tgataaatga gatcaatgcg taactgacgc taagtgcaac atgcccccag cgaggaatct
    27961 tgaacgtacg tgcaagaaac caaaaagcca atccggcaca gccgacctta agcaaagtca
    28021 gaacatacag tgcatctggc atcatctggt ttggaaaaaa gaacaccagt ggcgtgaaga
    28081 tcccgcctag atagtaagaa attaaagata aataattcaa tcctagagaa gcattccatg
    28141 tataaaaaat actttgcttt ccaagaagaa catttcggaa gctggcatga aaattagaaa
    28201 actgtgaaaa ggcatcactt gccaaaatac ttctgctgct tccaggatag attccgatac
    28261 ttagataaac aagtgctaaa atcaaaaacg gaatcaggaa actagctgct gcgtagcgtc
    28321 cgttttctct aaagaatgac tttaaatttt ttttcatatt tattttccac caaatttcta
    28381 tgaagttgta ttcagtttat catatccttg tttttctctg cttaaaaaga gtctagtttt
    28441 cacaaaaaaa taaaatttag aataaataac ttcataattt gttcgtataa caaaaataaa
    28501 cgcaaaaaaa gggtaggaat caaaagtccc acccttaaaa atttttatta aataaaaaac
    28561 cgttcactat ctgaacggtc caagtatcat tcaattaaac gcgttcaact tttcctgatt
    28621 tcaaagcacg agttgacacc caaacttttt taggttttcc gtcgaccata acgcgaactt
    28681 tttgtaagtt aggtttaaca gtacgttttg ttgagttcat agcgtgtgaa cggttgtttc
    28741 cactttttgt cttgcgacct gtaaagtaac aaacttttgc catggtaatt tcctcctttg
    28801 ccttgatttg gagcaataga ataattcagc tcataccaga ctaacatatc atatagaatg
    28861 ggaatttgca agtacatttt catagataca attgacaatt gagaagaata attgtaaatt
    28921 aaacaaggtt atacctgaaa ttttgcaagg tttttgagta ctttgggtaa ctgcctttta
    28981 tttcaagttg cgctatgata aaatatgata gaagaaaaat gggagttttt tacccgttaa
    29041 ggaggctcat tcatggctgt aaaaattcaa acgtcagctg gaaccattga aattaccaat
    29101 gatgtaattg caacagttgt tggtggcgcc gcgacagatg tgttcggtgt tgtaggaatg
    29161 gccagcaaaa atcaaattaa agataatatt actgagattt tgcgtaaaga aaattactca
    29221 agaggagtag ttgtccgcca agaagataat ggtattgctg tagatgttta tacgattatc
    29281 agctatggta cgaaaatctc tgaagtttcc cgcaacgtcc aagaaaaagt gaaatacaat
    29341 ctagaggcat tattaggcgt aactgccaat tcagttaatg tctttgtaca aggtgttcgt
    29401 gtactgccag actaggcagg agactggctg gtaactcagc agtcgatctg aaggaggatt
    29461 atctaggtga gtgtaacaga aataagtgca agccagttcc aggaaatggt ccaggcaggt
    29521 gcgaatcgtc tgcaagtaaa tgcagaatac gtcaattcat taaatgtatt ccctgtgcca
    29581 gatggcgata cgggaacaaa tatgaatcta tctatgacta gtggagctaa ggcagtagta
    29641 gattcaacct cagaaaaagt aggagaatta gcagcaatgc tttcaaaagg attgctgatg
    29701 ggtgcgcgcg gaaattccgg cgttattttg tctcaattgt ttcgtggatt ttctaagcaa
    29761 attccagatg ttgtctcatt aaatgcttct gatcttgctg cagccttcac tcatggagtt
    29821 gaaactgcgt ataaagcagt aatgaaacca gtagaaggaa cgattttgac agtagctaga
    29881 gaagcagcga aagctggaga aaagaaagca aaaagtactg atgatgtcgt tgaagtaatg
    29941 acagctgttg taaaaggagc aaagcgtgct ttagctaaaa ctccagactt gcttccagtc
    30001 ctaaaagaag tcggtgtcgt agacagtgga gggcaaggac tgctgtttgt ttatgagggc
    30061 tttttaagtg cattgaatgg agaattccaa gcggatgaaa catatgaacc ttctccagca
    30121 gaaatggatg atatggtcaa tgcagaacat catcgcagtg tccaaggtca aatggcgaca
    30181 gaagatatcc attttggtta ctgtacagaa atcatggtaa aaatcggaga aggacctaca
    30241 gtcgatagta cattcgatta cgaagaattc cgtaactatt tagatggtat aggagattct
    30301 ttacttgtgg tcaatgatga cgagatcatc aaagtccacg tgcatacaga aaacccagga
    30361 gaagtcttga attatggaca aaaattcggt tcattagtca aaatcaaagt cgacaacatg
    30421 cgcttgcagc atgagacgat tttggaacac gatgaagaag tgaacgagtt tgcgaaaaaa
    30481 gaaataccac gtatcccgta tggtatcgta gcgatcgctg ctggtaaagg cgtccaagag
    30541 ctgtttgaaa gtttaggcgc taattatgtt atcagtggtg gacaaacaat gaatccaagt
    30601 acggaagaca ttgttaaagc gattcaagaa gtaaacgcag ataaagtgat tgtcttgcca
    30661 aacaataaga acatcttcat ggcagctgat caagcagcag aagtagcaga tatcccagta
    30721 gcagttgttc cttcaagaac gatttcccaa ggattgacag ctatgctttc attcaatgat
    30781 caagcttctt tagaagaaaa caaagaaacg atgacagaaa tgttgtctag cgttgtgagc
    30841 ggtcaagtca ctcacgcgat cagagatacc tcaattgacg gtgtagagat caaagaagga
    30901 gactttttgg gcatgatcga tggaaaaatc ctgatctcta acccagatat cacagcaaca
    30961 gcgttaagta cactagaaca aatgatcaat gaggacacag agatcgtaac gatcttggca
    31021 ggtgatgaag gaacagttga acaagcaaat gaatttgctg agaaactaac agaaaaacat
    31081 cctgatttgg aaatcgagat ccaccaagga gatcagcctg tctatcctta tctattttct
    31141 gctgaataat ttctgtgtca gaatatcttt ctaacacagt attcattcat aaaaggcctt
    31201 cggttagtga tcaggtatga tcgctgacca agtccttttt ttgttaaagg agggaggaaa
    31261 aatttatgcg ttctttagat gatccaatca ctatgctggc aggagtcgga gaaaaaagag
    31321 cagctagtct tgccagcttg ggcatccaaa cgattgaaga cttactgact tattatcctt
    31381 ttcgctacga agatattcag gaaagaaatc taaacgagat ccaggatcaa gaaaaggtta
    31441 ctttgaaagg aatcgtagtc tcgcctcctg tgatgaatcg atttggttat aaaaaaagcc
    31501 gattgcaatt tcgtatgatg caagaacatg atgtgttcaa cgtttctttt tttaatcaac
    31561 cttatctaaa agaaaaagtc attttatcgg aggaaatcgc cgtatacggt aaatgggatg
    31621 caaagcgtaa agccttgaac gggatgaaga ttttaggttc tcaaacaatg gatgactttt
    31681 cgccgatata tcacgtcaac aaatcaattc gtcaaacgac attagttgat ctaatcaaaa
    31741 aagggtttca agaatacggc acattgattg aagaaaatct gccaaacaac cttgtggaga
    31801 aatatcgact gcttgaccga gcttctgctg taagaagtat gcattttcca aaagaccacg
    31861 aagaaaacca tcaagccaaa cgacgtgtcg tttttgaaga gttcttttta tttcaaatgc
    31921 gtcttcaagg attgaaaaaa gcagaaaaag cagaaacgaa tggtttggag atcctttatg
    31981 atgtccaaaa attgaaacaa tttactcaaa agctaccttt tgaactgaca aatgcacaaa
    32041 aacgtgtaac gaatgaaatc tgtcgggatc ttcgcagtcc gcagcatatg cagcgacttt
    32101 tgcagggaga tgtaggtagc ggaaaaacag tggttgccgc gattgcgtta tacgctaccg
    32161 ttacagcagg tttccaaggt gcgttgatgg tgcctacaga aatcttggca caacaacata
    32221 tggaaagctt gcaacagctg tttgatccca atgaagtaac catcgcattg attacggggt
    32281 caacgaaagc aaaagaacga cgtgagctcc ttgaaaaact agagcaaggg gaaattaacg
    32341 tcgtgatcgg cacacatgca ttgatccaag atggagtcga atttcagaat ttaggtctag
    32401 ttattacgga tgagcagcac cgttttggcg tgaaccagcg gaaagtactt cgtgaaaaag
    32461 gctggcgacc agatgtctta tttatgacag cgactccgat tcctagaaca ttagctatta
    32521 cagcatatgg tgagatggat gtatctatca ttgatgaact tccagctgga cgtatcccga
    32581 tcgaaacacg ctgggtaaga acacctcaat tggacagtgt actggattgg acgaaaaaag
    32641 aactggcaag aggacatcaa atgtatgtca tctgtccgct gattgaagaa tcagaagcat
    32701 tagatgtaaa aaatgcagtg gaaatctatg aaaaacttcg tgaactattt gcaccaaaat
    32761 atgaagttgg cttgttacat ggtaaaatga agaatcagga aaaagaagta atcatggaag
    32821 ccttcaaaga caaccagatg cagatattag tttcgactac tgtaattgaa gtaggggtaa
    32881 acgtgccgaa cgcgaccgtc atgctgatca tggatgcgga tcgatttgga ttggcacagc
    32941 tgcaccaact tcgtggacgt gttggacgcg gaagtgatgc ttcctactgt atcttagttg
    33001 ctaatcctaa aaatgaacta ggtgtggaac ggatgaaaat catgacagag accaccaatg
    33061 gttttgtact aagcgaaaaa gatctggaat tacgtggtcc tggtgaagta tttggcttcc
    33121 gtcaatctgg tctgcctcaa tttgcgattg ctgacttggt gacggatgga aatgttttgg
    33181 aggtagccag agaagaggct gcagcccttt ggcaaataaa ggattggcag atgcttcctg
    33241 agtatcagcc tttagcgcaa agcctccaaa tagaaaatca ggataatcgt tatttcgatt
    33301 agcagactgt gctatacttt atactgatga cggaaaaagg agtggaaaca atgaaaattg
    33361 ccgtagatgc aatgggcgga gatcacgcac ctcaagcaat tgtagaaggc gtggctttag
    33421 cgcagcaaga ttttccagaa gtagaattct tactttacgg aaaagaagca gaaatcaaga
    33481 aatacttaac aaatgaaaaa aacatcacga ttatccatac agacgaaaaa atcaatagtg
    33541 atgatgagcc agtaaaagca attcgacgaa aaaagactgc atcaatggtc cttgctgcac
    33601 aagcagtgaa aaacggtgaa gcagatgctg tattttcagc agggaataca ggcgcgctac
    33661 tagcagcagg gttgttcatc gtaggacgta tcaaaaatat cgaacgccct ggcttgatgt
    33721 ctaccttgcc agtagtaggg aaagaaggtg ccggtttcga tatgctggat cttggcgcta
    33781 acgcagaaaa caaaccagaa catttgttgc aatacggtat tttaggttct ttttatgcac
    33841 gtaaagtccg aggtatcgga cgtcctcgtg tcgcactctt aaacaacgga acagaagcaa
    33901 caaaaggtag tgaagtgacg aaaaaagcct atgaactcct tcagaatgaa acaagcttga
    33961 attttattgg aaacgtagaa gctcgtgagc tactcaatgg tgtggctgat gtagtggtca
    34021 cagatggatt tacaggcaac gctgttttga aatctattga aggaacagca atgaatatga
    34081 tgaatctatt aaaatctgct attctaaatg aagggatcaa aggaaaaatg ggcgctatgc
    34141 ttttaaaaga cggcttacgt tctttgaaag cagaaatgga ttattccaaa catggcggtg
    34201 ccgttttgtt tggcttgaaa gcaccagtca ttaaaactca tggagcaaca ggccccgatg
    34261 ctgttcgcta tacgatccgc caaatccaca caatgcttga gacagatgta gttggacaat
    34321 tagtcgaaca atttgaaaaa agcgaagaat agtatttgga tatctgaagc gatccgttcc
    34381 attgaaaaaa aggtggacat tcctgaaaaa tgccagtaaa attagccatg gtgtaggtaa
    34441 ttgacaagtg tatgattctt ggaggtgaaa aagttgactc gcgaagaagt atttaacaaa
    34501 gtagcgaaaa ttatttcaaa tcattttgag atagataccg ataaagtgac ggatgaattg
    34561 aacatcaaag atgatttaaa agctgattca attagtatta tggaattcgt tttagagctt
    34621 gaagatgaat tcggtacgga aatttcagat gaagacgctg aacaaattga aacagtcggt
    34681 ggcgcagtag attatatcag cagccatctt aaataattt
//
LOCUS       DAZUMK010000015        32533 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000015 DAZUMK010000000
VERSION     DAZUMK010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.015"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACG808_000759"
     CDS             131..505
                     /locus_tag="ACG808_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFS5046410.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACG808_000760"
     CDS             complement(619..1248)
                     /locus_tag="ACG808_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS5046411.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACG808_000761"
     tRNA            complement(1382..1454)
                     /locus_tag="ACG808_000761"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="ACG808_000762"
     tRNA            1668..1741
                     /locus_tag="ACG808_000762"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            complement(1879..2052)
                     /locus_tag="ACG808_000763"
     CDS             complement(1879..2052)
                     /locus_tag="ACG808_000763"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046412.1"
                     /translation="MNKMKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGM
                     LIYFVFSDDGKYG"
     gene            2338..3666
                     /locus_tag="ACG808_000764"
     CDS             2338..3666
                     /locus_tag="ACG808_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFS5046413.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="ACG808_000765"
     CDS             3868..4701
                     /locus_tag="ACG808_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFS5046414.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="ACG808_000766"
     CDS             4717..5454
                     /locus_tag="ACG808_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFS5046415.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="ACG808_000767"
     CDS             complement(5609..6577)
                     /locus_tag="ACG808_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFS5046416.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="ACG808_000768"
     CDS             6809..7642
                     /locus_tag="ACG808_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFS5046417.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="ACG808_000769"
     CDS             7676..8515
                     /locus_tag="ACG808_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5046418.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="ACG808_000770"
     CDS             8541..9101
                     /locus_tag="ACG808_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5046419.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="ACG808_000771"
     CDS             9300..11021
                     /locus_tag="ACG808_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFS5046420.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="ACG808_000772"
     CDS             11186..12328
                     /locus_tag="ACG808_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFS5046421.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="ACG808_000773"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="ACG808_000773"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFS5046422.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            14128..14775
                     /locus_tag="ACG808_000774"
     CDS             14128..14775
                     /locus_tag="ACG808_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFS5046423.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    14904..15120
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15168..17813
                     /locus_tag="ACG808_000775"
     CDS             15168..17813
                     /locus_tag="ACG808_000775"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFS5046424.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            18123..19436
                     /locus_tag="ACG808_000776"
     CDS             18123..19436
                     /locus_tag="ACG808_000776"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFS5046425.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            19426..20079
                     /locus_tag="ACG808_000777"
     CDS             19426..20079
                     /locus_tag="ACG808_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5046426.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            20134..20826
                     /gene="radC"
                     /locus_tag="ACG808_000778"
     CDS             20134..20826
                     /gene="radC"
                     /locus_tag="ACG808_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFS5046427.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            20976..21176
                     /locus_tag="ACG808_000779"
     CDS             20976..21176
                     /locus_tag="ACG808_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS5046428.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(21430..23109)
                     /locus_tag="ACG808_000780"
     CDS             complement(21430..23109)
                     /locus_tag="ACG808_000780"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFS5046429.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(23111..23323)
                     /locus_tag="ACG808_000781"
     CDS             complement(23111..23323)
                     /locus_tag="ACG808_000781"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFS5046430.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            23716..25446
                     /gene="efrA"
                     /locus_tag="ACG808_000782"
     CDS             23716..25446
                     /gene="efrA"
                     /locus_tag="ACG808_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFS5046431.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            25443..27203
                     /gene="efrB"
                     /locus_tag="ACG808_000783"
     CDS             25443..27203
                     /gene="efrB"
                     /locus_tag="ACG808_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFS5046432.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            27298..27495
                     /locus_tag="ACG808_000784"
     CDS             27298..27495
                     /locus_tag="ACG808_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046433.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            27648..28133
                     /locus_tag="ACG808_000785"
     CDS             27648..28133
                     /locus_tag="ACG808_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFS5046434.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(28237..28830)
                     /gene="clpP"
                     /locus_tag="ACG808_000786"
     CDS             complement(28237..28830)
                     /gene="clpP"
                     /locus_tag="ACG808_000786"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFS5046435.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            29010..29537
                     /locus_tag="ACG808_000787"
     CDS             29010..29537
                     /locus_tag="ACG808_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046436.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            29628..30365
                     /locus_tag="ACG808_000788"
     CDS             29628..30365
                     /locus_tag="ACG808_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFS5046437.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            30369..31286
                     /gene="miaA"
                     /locus_tag="ACG808_000789"
     CDS             30369..31286
                     /gene="miaA"
                     /locus_tag="ACG808_000789"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFS5046438.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            31288..32517
                     /gene="hflX"
                     /locus_tag="ACG808_000790"
     CDS             31288..32517
                     /gene="hflX"
                     /locus_tag="ACG808_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFS5046439.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT        10772 a         5522 c         6874 g         9365 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttgcga
    13921 aaaagctgcc ctgcatcaag taataagaac agccaaacaa aaatagttcc tcatatttta
    13981 cgtttggttg agcttattac cgcagatgca agcttttgaa caccgtttta tttgtctcac
    14041 acccttttcc ttcctacttg taaccttgct cccgtgttag aaacatgtta cactaaatat
    14101 gtgaaattat taacaaggag cgtcgttatg cgagaaaaaa cagatacact gccaaaagca
    14161 actgcgaaaa gattaccttt atatttacgc tacttgaaga tgctggatga ctcaggtata
    14221 agtagaatca aatcaaatga gttcagcgaa atcacccaaa ttccttctgc aacgatccgc
    14281 agagacttct cgcaattagg cgagctaggc agaagcggat atggctatga tgtccctttt
    14341 cttatcgatg tattcaataa tatccttaat actaaagaag aaaaacgaat cgcattagta
    14401 ggatatggaa atcttggcaa agcattgaaa cacaacaatt ttagaagaaa tgaaaatcta
    14461 aatatcgtgt gcgtctttga taatgatcca gcactgatca atcgagtaat tgatggagaa
    14521 atgatctatc cgattgatcg tttcgcggag atagccaaag caaaaaatgt gacagtagca
    14581 atctctacgg taccaagtaa atattcacaa tctgctattg atgaaattgt caaaggaaat
    14641 gtaaccgcta tacttaattt tgcgcctgac agagtaactg tcccagctta tgtcaatgtt
    14701 caatacattg atttgacaac cgagttgcag acgcttattt actttgatga gaattactca
    14761 gaagtttttt cttaggaaaa tttgagtagg cttattgatc ttgcgagaac tcgtcgtaat
    14821 tctttcaaaa attttttagt actaaatgaa gtggtttttt cacgaccact tgttgactaa
    14881 aaaaaaaaac cattgtagaa tgaagcataa cgctgaacaa gaggagtaac tggcacattc
    14941 attcttagag agagaactat ctgctgaaag gttctcaatg acaaccagtg aaggtagctt
    15001 gggagtttct gtcttgaatg gagtagagat agacgagtcg tgatcgttaa ctcacttgag
    15061 aggcaataat gattgattat tgcaatttag gtggtaccgc gtaaatcata cgtcctagaa
    15121 tacgaaagta ttttaggacg ttttttagtt taaaggagga aaacagcatg acggaagaaa
    15181 ataacctatc aacaaaatat aatccgcaag aagttgaagc tggacgttat cagcattggt
    15241 tagaacaaga tttgtttaaa ccgagcggag ataaaaaagc aaaaccttat tcaatcgtaa
    15301 tcccgccccc aaatgttact ggaaaacttc atttagggca tgcttgggat acgaccttgc
    15361 aagatatgct gattcgtcaa aagagaatgc aaggatatga tactttatgg ctgcctggta
    15421 tggaccatgc aggaatcgcg acacaagcaa aagtagaagc gaagcttaga gagcaaggga
    15481 tttctcgtta tgatctaggt cgtgaaaagt tcatcgatca agtgtgggaa tggaaagaag
    15541 agtatgcgag ccacatccgc gaacagtggg ctaaaatggg cttatcttta gattacagta
    15601 gggaacgttt tacattagat gatggattat ctgaagcagt acgtaaagtt tttgttacct
    15661 tatacgaaaa aggtttgatt tatcgtggtg aatatatcat caactgggat cctcaagcac
    15721 gaacagctct atctgatatc gaagttattc ataaagatat cgaaggcgcc ttttatcata
    15781 tgagctatga actagcagat ggatcaggcg tagtagaaat tgcgacaacc cgtccagaaa
    15841 cgatgttggg agatacggcg attgctgttc atccagaaga cgagcgttat caagcgctca
    15901 tcggcaaaaa agtgattcta cctttagtca ataaacaaat cccaatcatt gcagatgaat
    15961 atgtagatat ggaattcggg acaggtgtag taaaaatcac tccagcacat gatccaaacg
    16021 actttgaggt aggtaatcgt catgatcttc cacgcgtaaa tgttatggat gatgatggta
    16081 cgatgaatga actagcagga aaatatgctg gaatggaacg ttttgctgct cgtaaagcaa
    16141 tcgtgcaaga tctgaaagaa atcggtcgat tgattaaaat cgatacgatg atccacagtg
    16201 tgggtcattc cgagcggaca ggtgtcgttg ttgaacctcg gctgtctact caatggtttg
    16261 taaaaatggc gccgttagcg aaaaaagcca ttgataacca agaaacagat caagcagtgg
    16321 aattcttccc tccaagattc aatcaaacgt ttttacgttg gatggaaaat gtccatgatt
    16381 gggtaatctc acgacaatta tggtggggac atcaaattcc tgcatggtat cacaaagaaa
    16441 caggtgaaat gtatgtaggg atggaagcac cagcggatag tgaaaactgg gtacaagatc
    16501 ctgatgtttt agacacttgg tttagttcag ctttgtggcc tttctcaaca atgggctggc
    16561 cagatacgga aagcgaagat tataagcgat acttccctac tagcacttta gtaaccggtt
    16621 atgacatcat tttcttctgg gtaagcagaa tgattttcca aagcttggaa tttactggcg
    16681 aacgtccatt tgagaatgtc ttgattcatg gattgattcg tgacgaacaa gggcggaaga
    16741 tgagtaaatc attaggtaat ggaatcgatc cgatggaagt aatcgaaaag tatggagcag
    16801 atgctctccg ctggttctta tcgaatggtt ctgcaccagg acaagatgtc cgcttcagct
    16861 atgaaaaaat ggatgcttct tggaacttta tcaacaaaat ctggaatgct tcacgtttcg
    16921 ttatcatgaa tatcgaagga atgaccgtag aagagatcga tttatctgga gaaaaatcag
    16981 tggctgaccg gtggatcctt actcgtttga atgaaacgat cgaacgagta actgagctat
    17041 ttgatcgttt tgaatttggt gaagcaggtc gacaactgta taactttatc tgggatgatt
    17101 tttgtgactg gtacatcgaa atgagtaaag aagtacttta tggagatgat gctgtttcaa
    17161 aacaaaccac ccgtagcatc ttagtccata cacttgatca gatcctacgt ttattgcatc
    17221 caatcatgcc attcgttaca gaagaaatat gggaacatat tcctcatcag ggaaattctc
    17281 tagtagtggc agaatatcct gttgtacgtc cagagtttga tgatgaaaca gcttctaaag
    17341 ggatggaagt attgaaagaa ttgattcgag ccgttcgaaa tattcgttca gaagtgaata
    17401 caccattgtc taaaccaatt acgttgctaa ttaaaacaaa cgatgctaaa atcgatcaat
    17461 ttttagttga aaatacaagc tatatcgaac gcttctgtaa tcccgaagag ttgacgatat
    17521 caagtgagat cactgcgcca gatctagcga tgtcagctgt tctgacaggt gcagaaatct
    17581 tcttgccgtt agcaggattg atcaatattg aagaagaaat caaacgatta gaaaaagaac
    17641 tggaaaagtg gacaagcgaa gtaaaacgtg tccaaggaaa actttctaat gaacgttttg
    17701 tttctaacgc gccggaagaa gtagtagagg cagagcgtgc aaaagaaaaa gattatttag
    17761 agaagcaaac ggctgtcaaa gaacgaatcg aaagtttgcg tactattcaa taagaataga
    17821 aaaagagagt gtgaaatcag ctagctatta ctaaaagcta gctgattgaa catcgttaca
    17881 ggaaagagtt tgagcctcac tcttagcttc gattcataag gagatgtgac ataaattttg
    17941 tcgcatctct tttttagtct actcttatta tgtaggagaa ggagggaggt ggatcatgta
    18001 tcaagagtca ataaagtagt tgataaaatc gttcctctct ctgtttcttt tctctttgtc
    18061 atacttttat ttttcttatg tgttctattt gttataataa aagagaaatg ggggaattgt
    18121 tgatgacgat tgaagaagca atagaatgga tccatagtcg attaccattt ggttcacgtc
    18181 ctggccttga ccgagtcgaa gctttattag aagtggtagg gcatccagag agaaaagtaa
    18241 aaacgatcca tatcgctgga acaaacggaa aaggttctac tgtagcttat ttgagaagcc
    18301 tattagaaga ggctgggctg acagtaggta catttacttc accttatatc gaaacgttca
    18361 atgaacggat cgcaatcaat ggcaaaagca ttccagacaa tgatttgatc cagcttgtaa
    18421 aaaaatatca gccactgatc aaacaacttg atcaatttga agcagtagct ggtattactg
    18481 aattcgagac attaacagca atggcatttg attatttttt gatgaagaaa gtggacgtag
    18541 cgattatcga agttggctta ggcggcttat tggattctac caatgtagct gtaccaatgc
    18601 tgacgggaat aacaacgatt ggacttgatc ataccgaaat acttggagat acaattgaag
    18661 aaatcgctgc acaaaaagct ggaatcatca aacaaggcgt tccagtagta acaggaaata
    18721 ttgccccaga agcttttact gtcatccaac aaacagctaa agcaaaaaat gcaccgcttt
    18781 atgtatacgg ggaaaattat aaaataaact atctgcatcc tgatgagatg tggggagaag
    18841 tatttgaatt ttttaatgaa ataggaaaga tccctcaatt aaccactccg cttttagggc
    18901 aacatcaagc tgaaaatgca ggagtagcca tcgaattgta ttttgtttac tgtcaattgg
    18961 aaaagctgcc tttccgagaa aaagatgttt tgaagggatt gaagaaatct cgatggccag
    19021 gaagaatgga gcgtttgagt aacgagccat tagttgtttt agatggtgcc cataatacgc
    19081 atgcaatgaa ccggcttgtt gagaatttga aaaaagaatt caaaggatat caaatcaaca
    19141 ttttgttttc cgccttacga actaaaaatg ttagagaaat gctagaacga ttgcagcaag
    19201 taccaaatgt ccatatttat ttgacgacct ttgaacatcc aaaagccatc gaattaagtg
    19261 aagtatcaga tttagcaagt gagagagtat caatcgtttc attgtggcaa ttcggattag
    19321 cagatatatt ggaaaagatg acaaatgaag acgtattagt tgtgaccggt tcactttatt
    19381 tcatttcaga agttcgccat ttattaatac aattaggagg agatcatgaa gtataatgca
    19441 gtgatttttg acatggatgg tttgctgttc gacacagagt tagtgtatta cgaagccaca
    19501 caagtagtag cagacaagat gggttttcct tatgataaag acctgtattt aaaattctta
    19561 ggtgtttcag atgaagaagt atgggcgaat tatcatatga ttttctcgga atttggcaaa
    19621 acaagtgtac aaagattcat agatgattca taccaggaaa cgcttgaacg tttttcgcaa
    19681 ggagcggtac aaatgaagcc cggtgtgttg gaactcctct cttttttaga agaacatcag
    19741 attcccaaag ttgtcgcttc cagtaaccaa cgagcagtca ttgaactttt attgaagaag
    19801 aacaatctgt ttgaacgatt tgagacgatt gtttctgcag aaaatgttca acgagcaaaa
    19861 ccagacccag aaattttctt gttagctaat cagaagctag gtacagcgaa gaaagaaaca
    19921 ttgattttag aagattcaca aaatggtatt ttagcagctt ctgcggctga gatcccggta
    19981 gtcatggtac cagatctgct tccaccatcc caagaattga tcaaaaaaac agctggggtt
    20041 gtttcttctt tacatgaaat ccctgcattg ttaaaataaa aatccatctc ttttcgtatc
    20101 ttattttaga taagatagaa aggagatgtt tttttgggaa aacgattgat caaagaagta
    20161 cctactagct ctttaccaag ggaacgtatg gaaatttatg gcgcggaagc tttatcggat
    20221 caggagcttt tggcaatctt actgcgtacc ggtcaacacc cttatagtgt gatgtcgatt
    20281 gctggaaatc tgttaaaaac gtttggagga ctggcctctt tacgacaggc tactttgcat
    20341 gagttagaag agattcgagg gataggacgc gtgaaagcaa tcgaaatcaa agccttgatc
    20401 gaattaggtc gacgaattca gacggatcat gaaagagcgg ctccaccggt tcgtaccagc
    20461 tacgaattgg cccagcaatt gattctggaa atgaaagatc acaaacaaga acatttggtt
    20521 tgtatttatt tggacacgaa gaatcaagtg atcttaaaaa aaacagtgtt catcggttcg
    20581 ctgaatcagt cagtcgctca tcctagagaa attttccatt atgctgtacg ttattgtgca
    20641 gcaaggatcg ttttagcgca taatcatcct tctggtaatg ttattccatc gcagcaggat
    20701 atgaatttta ccaaaaggat acaaaagtgt ggagaaatga tggggatcac tgtactggac
    20761 cacttgatca tcggacgcaa acgatatttt agtttgagag aagaaggaat gatggaagag
    20821 aagtaagttg ctttcttgca aaaaaaatga agcaagtgta caattatatt gtaattttgt
    20881 ttgggaatag aagttgaaac atctgttgca cctctatcgt acacgagcaa tgttacaatt
    20941 taaattagtg cttttggcac gaggaggata ttattatgga aaacggaaca gtaaaatggt
    21001 tcaacgctga aaaaggattt ggatttatct cacgtgaaga tggaagcgac gtattcgttc
    21061 acttctcagc tatccaaggt gacggattca aaacattaga agaaggtcaa gcagtgactt
    21121 tcgacgttga agaatcagac cgcggcccac aagctgcaaa cgttgtaaaa gcttaatttt
    21181 atctgtaaat caaattgatg gtagacatca atttttacga gagaacaaag cgagtaacaa
    21241 aaatacctgc cacaagagac aatttctctt gtggcaggta tttttttgta tgaaaaagta
    21301 aataaattta agaggctatg aaagagccac cttgtatcaa ggaataagaa caaacaaaaa
    21361 tggcttctca tgtttttcat ttgttttctc ttatattcag gacaaggtgc tgatttcata
    21421 acctcttatt tattcttcat ctggtgtcat taccattggc aaaatcattg gatgtctttc
    21481 tgtacgttcg aataagaaag gctgaagcgc gccagtcatc gcttctttca atttatattc
    21541 tgaacagtca ggtccattca aacattctct taaagcgtgg aataagattc gctgagcttc
    21601 attgatcatt tctcctgatt ctcgcatgta gacaaatcca cgagagagaa tatctggacc
    21661 agccaagatt tcttttttct tgatatccac tgtcgcgaca gctaatacaa gaccttcttc
    21721 tgaaagcagg tgacggtcac gtaaaacgac attaccaata tcaccgatcc cgctaccatc
    21781 gatatacaca tctccagcca caaaatgccc agcaggacgc gcagaatcgc tagttaacgc
    21841 taaaacatcg ccattttcca tgatgaagca gttttctgca ggaacaccta catcttgtgc
    21901 tagagaagca tgtactttca acatgcggaa ttctccgtgg acaggaataa aatattttgg
    21961 cttcatcaaa cgaagcatta atttttgttc ttcttgtcca ccgtgaccgg aagtatggat
    22021 gttattgatt tttccgtgaa tcacttctgc accagcttca gacagcaaat tgatcaaatg
    22081 attgacactt gttgtattac caggaatagg tgaacttgaa aagatcactg tatcgtctgg
    22141 ctggatttgg atttgacggt gagtaccatt cgcgatgcgg ctcaacgcgg ccatcgcttc
    22201 tccttgagaa cctgtacata ggatcatcgt ttcattggca ggcaagcgat tgatttcgtg
    22261 tgcatctaca aatgtgccgt ctggaacttt gatatatcct aaacgttgac cgttgacgat
    22321 ggcattttcc atgcttcgtc cgaaaacagc gattttacga ccagttgcca cagcggcatc
    22381 tgctgcttgc tgcaaacgga agatgttaga agcaaagctg gcaaaaatga tacgtccttc
    22441 gatttttgaa aagatcttca aaatagaatg cccaatcgtt ttttctgatt tagtaaatgt
    22501 tggaatctca gcatttgtac tatcagataa aaggcacaag actccttctt ccccgatacg
    22561 agccatttta tgcaagtttg ctggttcgcc aacaggagtg aaatcgaatt tgaagtctcc
    22621 agtcgctacg atatttccag aaggtgtttt caccacgatc cctaatgcat ctgggatact
    22681 atgggtcgtt ctgaaaaagc tgacagaagt ctttctaaaa cggatgatcg tatcttcatt
    22741 gatctcatgg agctctgcat ctcgaagcaa gccatgctca tctaatttgt ttgtgatcaa
    22801 cgctaatgcg agaggaccag catagatagg aatatttgct tgtttcagca agtagggtat
    22861 tccgccgatg tggtcttcgt gcccgtgagt aatcaccaag gcttttactt tgtgtaagtt
    22921 ttgtatgatg tagctgtagt cgggaatgac atagtcaatt ccaagtaaat cgtcttccgg
    22981 gaatttgatc ccggcatcaa tcaaaatgat ttcatcttgg aattgtaccc catacgtatt
    23041 tttcccgatt tcacctaatc caccaatagc aaagacgcct gtctcgttat ttttgatgga
    23101 aagtttcata ttaaaactcc actaatgaga aatcagcatt ttctttttcg tactctagat
    23161 gacttccttc tagaagttga acgaattcaa tattgtatgg ggtattttct tccacacgtt
    23221 ggcgagcgtc tacatcgctt tttgcctcaa tgtaaagaga ttttgtttct tcccgtttag
    23281 ggttgcgtac ttttgtttct tgataataaa ctttatagat cattgttttg tctcctttat
    23341 ctgcgtaaag cagtgattta gctagttgat ttttttagac aacacaaaac gaggggacaa
    23401 gccacctggt ttttcatatg ctttgactag aatactggat tcgagtcaaa gagacaagcc
    23461 tcgtttttgc ttatctgttc ggatattttt tcaaccgtac cttctgtata aaaaatatac
    23521 aacaattaat tgttattcta gcataaaaca gcaaataagt atagaaatca actagattga
    23581 aagaaaaaat caatgtttat catcccgcta taatcataat gactttttta tgagagaagt
    23641 gttctattaa taggctcgtt gtgctatcat ttttagtaat gaaaatgtaa caaaagatta
    23701 aaaggagatg agaaagtgaa actaatgtgg cattatacca tgcgttataa gaaattcttg
    23761 tttttcaatt tcatatgcgt atttggtttt attttgattg aattaggact tcctacgatt
    23821 ttagcacgta tgatcgatgt aggaatccgc aatgatgatt ttgattatgt gaagcaacaa
    23881 gggatattta tgatcgttat tactgtaatc ggtattattt tgaatatctt gttaggttat
    23941 tttggttcga ggattacgac taatatcgta gcagatattc gagatgattt gttcgaaaag
    24001 atccaaagtt attcgcatca ggaatacgaa aatctaggcg tatcttcatt gatcactcgt
    24061 acaacaaacg atgcttatca gatcatgttg ttcctgcaaa atattttacg tatcggtttt
    24121 atggcaccaa tgatgttcat tgtcagtttg tacatggtca tgcgtacaag tgtgacactc
    24181 agtttgtatg tagtaggtgc attaccttta ctattattgg cagtcgtagc gattgctaag
    24241 gtgtctgaac cgttatctaa aaagcaacaa aaaaacttag ataaaatcaa cagtatcttg
    24301 cgggaaaatc tttcaggatt gcgtgtgatt cgtgcattcg tcaatgaaaa gtttgaagaa
    24361 aaacgttttg ctgtagtcaa tgagaattat gcttctagtt caaaaagctt attccgtttg
    24421 atggctgttg cacaaccggg tttttatttc ttatttaata tcgtaatggt attgatcatc
    24481 tggagcgggg cagtgcaaat cgatgcaggc gagcttcgtg taggagattt gattgcgttt
    24541 atcgagtata tattccatgc cttgttctca ttcatgttat ttgctagtgt gttcatgatg
    24601 tatccaagag cagctgtttc agctggacgt atccaagaag ccctagatgc caaaccttcg
    24661 attgtagaag atccaaatgg tatatctgat acaaaaacta aagggtatat cgaatttcaa
    24721 aatgtaacat ttgcttatcc aggccattcg caagagccag tgatacgaaa tgtcagtttt
    24781 acagcctctc caggagagac cgttgctttt attggaagta ccggtagcgg aaaatctacg
    24841 ttgatccaat tgatccctcg cttttatgat gtgagtgaag gaaagatctt gatcgatgga
    24901 gtagacgtga gagattatcg gctaagcaag ttgcgagata agattgggtt tattccgcaa
    24961 aaagcgctgc tatttactgg aacgattgca gataatttgc gatacgggaa agaagatgct
    25021 acgcaagaag aaatggaacg tgcagctgag attgctcaag cttcagaatt catttctcga
    25081 aaaccagatg gatttaatga acatttatca gaaggtggtt ccaatttttc aggtggacaa
    25141 aaacaacgat tggcaatagc cagagccatc atacgccgtc cggaaatcta tatttttgat
    25201 gacagctttt ctgcattaga ttatcaaacg gatgcgaagt tacgtgctcg tctgaaaaaa
    25261 gaaacgacag aatctactgt attgatcgta gcacaacgtg tgggaacgat catgcatgca
    25321 gataagatca tcgtattaaa tgaaggagaa gtcgtcggta tggggacgca tcgggagttg
    25381 ttggaaaaat gcccgattta ttatgatatt gccgcttctc aattatcaaa ggaggaatta
    25441 gcatgaaaaa aaccttttct tccttaaaac gtttagctcg atatatcaga ccgtatcgtg
    25501 agacttttct tttagtgatc ctatttacag ttctgacagt ggctttcaac gtggctttgc
    25561 catatgttac aggacttcct actacagaaa tcagccgtaa tattgccaat ggcgaatcaa
    25621 tcaatttttc ttatatattc aaatgtctca tttggatcac agttgttgga atagggtatt
    25681 gtatttcgca attactttct ggtgttttga tgacaaatgt cgttcaaagt gccatgcatg
    25741 atttgagaag agacatagaa gaaaaaatca atcgtcttcc ggtttcatat ttcgataaaa
    25801 accagcaagg aaatattttg tctcgtgtga cgaatgatgt agatgcagtc agcggtgctt
    25861 tacaacaagc ttttattggt atcgtaaatg cgatattggg aattacgttg gctgcagcaa
    25921 tgatgtttta tatccagccg atgatggcgc tgatctctat gatcatgatt ccaacatcga
    25981 tctggatctc aaaacgagtg atcaatgctt cgcagaaata ttttcaaagc atgcagaatt
    26041 ctcttggtga gctaaacggc tacgtccaag aaaatatgac aggttttagt gtattgaaag
    26101 tatatggacg cgaaaaagaa acattggaag gattcaaaaa agttaatcac agcttgaaat
    26161 actttgggtt tcgtgcagca ttcatttctg gcttgatgat gcctctagtt caattaactg
    26221 cctatgctac ttatattggt atggctgttc taggaagctt ttatgttatt actggggcga
    26281 tcgttgtcgg tcaactacaa gcattcatcc aatatatttg gcaaatcagt cagccaatgg
    26341 ggaacgtgac acaattgtct tcagttttgc aaagtgcatc agcggcaacg aaacgtgtat
    26401 tcgagatttt agatgagcca gaagaaaaag aaaacaaagt agatgcttca ctgccagaaa
    26461 cgattttagg agatgtgaca ttcgatcatg tcgattttgc atatgatccg aagaaaccat
    26521 taatccaaga tttgaatttt gaagtgaaag ctggtcaaac cgtagctgtg gtcggtccga
    26581 ccggtgccgg caagacgaca ttgatcaatc tattgatgcg tttttatgac gtagacaaag
    26641 gagcaataag gattgatgga atcgacacca aatcaatgag tcgcagtgat gtgcgttcgc
    26701 tatttggtat ggtcttgcaa gatgcatggc tttatcaagg aacgattgca gataatatcc
    26761 gcttcggaaa gcttgatgcg acagattatg aagtagttga tgctgcgaaa acagcaaacg
    26821 tagatcattt tatccgaacg atgccagatg gatatgatat ggccatcaat tctgagggag
    26881 acaatgtgtc tttaggacaa aaacagttgc tgacgattgc ccgagcagtg gtctccgatc
    26941 ctaaaatctt gattttagat gaagcaacaa gttcagtaga cacccgttta gaagcgttga
    27001 ttcaaaaagc aatggatcgc gtgatggagg gacggacaag tttcgtcatc gctcaccgtt
    27061 tatcgacaat ccgtgatgca gatctgattc tagtgatgga tcaaggtcag atcattgaaa
    27121 aaggaacgca tgagagttta ttggcacaag gtggatttta tgaaaaactg tataacagcc
    27181 aatttgctga ggaagcagaa taagtcatat aagaataaaa cagtttaaaa cttatttttt
    27241 gttctctatt cttgataatg gtaaagtaaa gttatcaaga aaagtgagga cacggagatg
    27301 gaacagtttt taaatgaaaa aattactgaa gccaaaagag aagttgatcg tttaacgcac
    27361 gtctgtcaga cgaagccttt tgattcagct gatttttttg aaaatgagtt tcaaatcgaa
    27421 cgcttggcag cacaaataga agtgtacgaa ttaattttaa aaaaagtaat agaagaaaat
    27481 atagatgtac aataaaaaca ggtaaaacgt cagttaccag atactgtaaa tagacatctg
    27541 gcctgagcag gttttacctg ttttttttct tatgaaataa atatatgcta tgataccttt
    27601 attcaatagg attagtttct acaaaaaatg aaaggagaaa aataaatatg gaacggttta
    27661 tagcatcaaa tgccacagtg attggtgatg tgacattgtc agaagatgtg acgatctggt
    27721 atcaagctgt tttacgcggg gacagtaatt ggataaaaat aggacaaaga acgaatatcc
    27781 aagatggtac aatcatccat gtcgatcatg atgcacctgt agatattgcg gagaatgtga
    27841 cggtaggaca tcagtgcatg ctacatggct gtacaatcga aaaaggggcg ctgatcggga
    27901 tggggaccac gatattgaat catgcggtta ttggtgagaa tagtttgatt ggtgctggat
    27961 cattagtaac agaaggaaaa gttattcctc ctaatgtctt ggcttttggt cgcccagcta
    28021 aagtgatccg tcctttgaca aaagaagaaa tacaaaaaaa caaagaaaac atccagcatt
    28081 atgtcgagat aggtcggatg catgcgcaag gaaaattcaa ggagcaaaga taggcagtag
    28141 catccataaa ataaaaaagg atggtcagtt tgagttgacc atcctttttt atttggttta
    28201 gataagagag cttaaaaaag cttcctttga aacagattaa tttaatgaag aactgttttc
    28261 catgacttca tcgatcaatc catatgcttt tgcttgttca gctgtcatat agttgtcacg
    28321 atctgtatct ttttcaatca cttcaagcgg ttgtccagtg cgttcagcta aaattttgtt
    28381 taagcgttcg cgtgtttgca aaatatggcg tgccgcaatt tcgatttcag ttgcttgccc
    28441 ttgagcgccg ccaagaggtt gatggatcat gatctcagca tttggtaaag caaatcgttt
    28501 accttttgta ccagctgtca gcaagaagct tcccattgaa gcagccatac ccataacaat
    28561 tgtttggaca tctgctttga caaagttcat tgtgtcgtaa atagccatac ctgctgtaac
    28621 tgaaccacca ggagaattga tataaagata gatatctttt tcagaatctt gagcgtctaa
    28681 gaaaagcaat tgggcgatga tcgaattagc aagatcatct gttacttggc cgcttaacat
    28741 gatgatacgg tctttcaata aacgtgagta gatatcgtaa gctctttcac ctcgtgaaga
    28801 ttgttcaatg actgtaggaa ttaaattcat aatagagttc ctccttgatt tcgtttagaa
    28861 atagtgtacc ataatttagt ttctaatacc taaaatgtag tatacatttt tggtcaaaaa
    28921 aggtcaaata aaaaacttag gcaaattagt cgcataaacc cgctttatgg tatgataagc
    28981 agagaaacaa aacaaggagg aacaacagat tgatagttgg tattactggc cttatcggag
    29041 tgctgacagg tgctgtagtt gtctatatcg gcatgatact gcaaatgcgt cacttggaaa
    29101 ataaagaaca gcgtagacgt gaattggaat tgaaagtcaa agaaatcgag acgctgaatc
    29161 aattaaataa aaagatcaac gagatcttgc aaaaacggac aacgctgatg gaagaatatg
    29221 tcagctttga tgcatttgat gattgttata tcacgattga cgattttgcg tatctccaat
    29281 cattttcagc gcaaaatagt ttttatctgc caaattatat tttagacgag ctatttaaga
    29341 atatctccca tcgaaaagtg attttatctc cagatgaaac aatgaagatt ggtggatatg
    29401 cttataaagg cggacgcgtg attttagaga attttacgga tagtctgact gaaatgatca
    29461 atgaaaaaaa agctcagttg aagactatga cgcgccagcc gattggtttc ttctcgaaag
    29521 atgatgtttg gagctgatat tttgaagcat tgccgttgca catgagagcg tggtaatgct
    29581 ttcttttttt cttaatgata caatagaact gaacggagtg aagaaagatg ttacaaatct
    29641 atgcccatcg cggaagcagc ggcacccacc cagaaaatac gcttcctgca tttgcagaag
    29701 ctgtacgagt aggtgcagat ggtatcgaac tagatgtcca tctaagcaaa gatggttatt
    29761 tgatcgttat gcacgacgaa gaagtggacc ggacgactaa cggtaaaggg ctgattcgag
    29821 aaaaaacatt ggaagaactc aaaaaattga atgcaggggg caaattcagc aaagaatttc
    29881 cagcagctaa agttcctgaa ttgagagaag tgttaggact gctcctccaa aaaaattacc
    29941 gagggatgct aatgattgaa attaaaactg atcagtatga atatcccggt atcgaagaaa
    30001 aattagccga aacattaaaa agtagagatt ggccttttca acatgcatat tgcagctttc
    30061 atttggattc tttggaaaaa atcgcattac tggaaccgga gatacagctg gacttattga
    30121 tgagtacttc tgataaaaag atccaactag cagcagaaac cccttatata gaaggagtac
    30181 atccaaaaaa tacttggttg ttcgaacatc cagtccaggc agctgatttt ccaaaagcaa
    30241 ttcgtgcatg gacgattaac gatgaagaag aaatggccaa atgtcaagaa ctcaaaatag
    30301 atggaataat aactgatttt ccggagaaag ctcttcggtg gaaacaatca acgaaaagta
    30361 ggtaaaatat ggaaaaagta ttagtgatcg tcggtccgac agcagtcggt aaaacagcgc
    30421 taagtgtgga actcgcacaa ctatttggag gagagatcat cagtggtgat tcattgcaaa
    30481 tctataaaaa tctggatatc ggtacagcaa aaatcaaaga agaagagatg aataatgttc
    30541 cgcatcattt gattgatgtg atcgaaccta ctcaacgtta ctcagccgct gattttcagc
    30601 attcaggtcg acaattgatt accgagattg cgaaccgagg gcacctacct atcatagctg
    30661 gtggtacggg tctctatatc caatcccttc tatatgatta tcagttagga gcgacagaag
    30721 aagcgaatcc tgagattaga aaaaagtatg aaacgttagc tgaaacaatt ggttcccaag
    30781 cattatggaa tcagttgaaa gaaaaagacc cggcagctgc tgagaaaatc cactggaaca
    30841 atcaacgcaa agtgataaga gctctggaag ttttagagac gacaggacat agtatcacgg
    30901 cacctaaaga agagccaaag caattgtatg attacctcat gattggatta gatacgaagc
    30961 gtagtattct ttatgaacga atcaatcaaa gagtcgattt gatgctagca gaaggtttag
    31021 tagcagaagc gcgtaaggtg tatgagcttg gagaagtcca ggccagtcaa ggtattggat
    31081 ataaagaatt tttcccttat tttgaaggga aagaaacatt ggaagaagca acagaacaag
    31141 tcaaactaca ttcgagaaga tacgcgaaac gtcagttgac gtggtttcgt aaccgtttgg
    31201 atgcacagtg gtttgatctt ataagtgagc caaatcaaaa aagtcagatt gagcaaacaa
    31261 tcaaaaaatg gctggaggcg gaataaaatg gaagaaaaag tgatcatcgt aggggtagaa
    31321 accgaagcaa accagcgtta cttttcagag tcaatggaag agctagttca gctgacgaat
    31381 accgcatcag gagaagtcgt attcacaatt acacaaaaga gaccacaagt ggatcggcaa
    31441 acgattatcg gtaaagggaa attacaagaa ttagttcaac aagcggatgc gcatgaagcg
    31501 gatttgatca tctttaatca tgagctaact ccgagacaga gccaactgat taccgatgct
    31561 gttggtcttc cggtaattga tcgcgtgcag cttatcttag atatttttgc gatgcgtgct
    31621 cgttcgaaag aagggaagct gcaggttgaa cttgctcagt tagaatacct gctgcctcgt
    31681 ttagtagggc aagggaaaac attatctcgt cttggcggtg ggatcggaac aagagggcca
    31741 ggtgaaacaa agttagaaac agaccgccga catatacgga ataaaatttt tgctgttaag
    31801 aaagaattga aagaagtaga agctcatcga gagcgcaatc gccaaaaaag aaaaaatagc
    31861 gagatttttc agattggact tattggctac acgaatgccg ggaagtcaac tatcttgaat
    31921 ctattgacac aagcggatac ttattcgaaa gatcaattgt ttgcaacgtt agatccattg
    31981 actaagagat ggcgttttgc tgaaggattt gagattactg taacggatac cgtcgggttc
    32041 atccaagacc tgcctacgca actgatcgat gctttccatt ctacattaga agaaagtcaa
    32101 aatatggatc ttttgctaca tgtagtcgat gctagttctc cagatcgtat tttacaggag
    32161 cagactgtct taaaattgat ggatgaactg aatatgaaag aaatgccgat actgactgtt
    32221 tacaataagg cagaccagat cgacccagcg atgttcacgc ctacgctatt cccaaatgta
    32281 ttgatttcta ctcagacgaa agaaggtaag atggcgttga tcgaagcaat caaacgtcag
    32341 ctgatggaac taatgacccc ttatacactt tctgtgccaa gcaacgaagg acagaaatta
    32401 agcgagttga gaagacaaac aatggttttg gacgaagcgt acatggaaga aacaaacgaa
    32461 tatgaagtta aagggtttgc aaaaaccaat tcaaaatggt taagaaaaaa ccaataaaat
    32521 tttatttttt taa
//
LOCUS       DAZUMK010000016        30019 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000016 DAZUMK010000000
VERSION     DAZUMK010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.016"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            205..903
                     /locus_tag="ACG808_000791"
     CDS             205..903
                     /locus_tag="ACG808_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFS5046440.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            1081..1422
                     /locus_tag="ACG808_000792"
     CDS             1081..1422
                     /locus_tag="ACG808_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046441.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            1415..2086
                     /locus_tag="ACG808_000793"
     CDS             1415..2086
                     /locus_tag="ACG808_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFS5046442.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            2092..2778
                     /locus_tag="ACG808_000794"
     CDS             2092..2778
                     /locus_tag="ACG808_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFS5046443.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            2781..3530
                     /locus_tag="ACG808_000795"
     CDS             2781..3530
                     /locus_tag="ACG808_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5046444.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            3542..3811
                     /locus_tag="ACG808_000796"
     CDS             3542..3811
                     /locus_tag="ACG808_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046445.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            3816..3980
                     /locus_tag="ACG808_000797"
     CDS             3816..3980
                     /locus_tag="ACG808_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046446.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            3973..4275
                     /locus_tag="ACG808_000798"
     CDS             3973..4275
                     /locus_tag="ACG808_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046447.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            4272..4433
                     /locus_tag="ACG808_000799"
     CDS             4272..4433
                     /locus_tag="ACG808_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFS5046448.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            4430..4735
                     /locus_tag="ACG808_000800"
     CDS             4430..4735
                     /locus_tag="ACG808_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046449.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            4735..5091
                     /locus_tag="ACG808_000801"
     CDS             4735..5091
                     /locus_tag="ACG808_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFS5046450.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            5051..5296
                     /locus_tag="ACG808_000802"
     CDS             5051..5296
                     /locus_tag="ACG808_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046451.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            5293..5712
                     /locus_tag="ACG808_000803"
     CDS             5293..5712
                     /locus_tag="ACG808_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFS5046452.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            5709..6266
                     /locus_tag="ACG808_000804"
     CDS             5709..6266
                     /locus_tag="ACG808_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFS5046453.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            6263..6559
                     /locus_tag="ACG808_000805"
     CDS             6263..6559
                     /locus_tag="ACG808_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046454.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            6636..7049
                     /locus_tag="ACG808_000806"
     CDS             6636..7049
                     /locus_tag="ACG808_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFS5046455.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            7358..7510
                     /locus_tag="ACG808_000807"
     CDS             7358..7510
                     /locus_tag="ACG808_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046456.1"
                     /translation="MKLSVEGTPEEIQELLQAIGSSKEQKVSVSNSDIDGICNCLNRE
                     KISNPY"
     gene            complement(7507..7782)
                     /locus_tag="ACG808_000808"
     CDS             complement(7507..7782)
                     /locus_tag="ACG808_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046457.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            8236..8442
                     /locus_tag="ACG808_000809"
     CDS             8236..8442
                     /locus_tag="ACG808_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046458.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            8638..8805
                     /locus_tag="ACG808_000810"
     CDS             8638..8805
                     /locus_tag="ACG808_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFS5046459.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            8831..9175
                     /locus_tag="ACG808_000811"
     CDS             8831..9175
                     /locus_tag="ACG808_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFS5046460.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            9180..9461
                     /locus_tag="ACG808_000812"
     CDS             9180..9461
                     /locus_tag="ACG808_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046461.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            9564..9878
                     /locus_tag="ACG808_000813"
     CDS             9564..9878
                     /locus_tag="ACG808_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFS5046462.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKINIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            9856..11550
                     /locus_tag="ACG808_000814"
     CDS             9856..11550
                     /locus_tag="ACG808_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFS5046463.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            11570..12748
                     /locus_tag="ACG808_000815"
     CDS             11570..12748
                     /locus_tag="ACG808_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS5046464.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            12711..13397
                     /locus_tag="ACG808_000816"
     CDS             12711..13397
                     /locus_tag="ACG808_000816"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFS5046465.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            13397..14557
                     /locus_tag="ACG808_000817"
     CDS             13397..14557
                     /locus_tag="ACG808_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFS5046466.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            14567..15442
                     /locus_tag="ACG808_000818"
     CDS             14567..15442
                     /locus_tag="ACG808_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046467.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            15439..15750
                     /locus_tag="ACG808_000819"
     CDS             15439..15750
                     /locus_tag="ACG808_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046468.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            15740..16093
                     /locus_tag="ACG808_000820"
     CDS             15740..16093
                     /locus_tag="ACG808_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFS5046469.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            16083..16484
                     /locus_tag="ACG808_000821"
     CDS             16083..16484
                     /locus_tag="ACG808_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046470.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            16477..16881
                     /locus_tag="ACG808_000822"
     CDS             16477..16881
                     /locus_tag="ACG808_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046471.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            16893..17501
                     /locus_tag="ACG808_000823"
     CDS             16893..17501
                     /locus_tag="ACG808_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS5046472.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            17521..17883
                     /locus_tag="ACG808_000824"
     CDS             17521..17883
                     /locus_tag="ACG808_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046473.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            17886..18068
                     /locus_tag="ACG808_000825"
     CDS             17886..18068
                     /locus_tag="ACG808_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046474.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            18085..21516
                     /locus_tag="ACG808_000826"
     CDS             18085..21516
                     /locus_tag="ACG808_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS5046475.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDFIVAIGGLTAAFGILLPAILAVSYLFGPMML
                     IIGGIIAVIAGVIVAIKNWGAITDWFSGLWKKFTDWLGDTWESIKDGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPEFIMEGSF
                     NPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGVGEAGPEAVLPLKRSVLQEIGDRILS
                     STSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
     gene            21567..22304
                     /locus_tag="ACG808_000827"
     CDS             21567..22304
                     /locus_tag="ACG808_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS5046476.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            22314..24605
                     /locus_tag="ACG808_000828"
     CDS             22314..24605
                     /locus_tag="ACG808_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFS5046477.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVDGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            24629..26755
                     /locus_tag="ACG808_000829"
     CDS             24629..26755
                     /locus_tag="ACG808_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046478.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSENPNLMPYITEPWVG
                     PLLGNGHTVKDSVKRVITHTKTRTANSGDILSLGLGIPCTAEANNRYLITTLRPSTTY
                     TLSVTMSVGSDWTGETNTIGVRLRYLNEQGGIELPINALIPANVERDKMVTHTFTGIT
                     KDNVTSITNCYVEIFSLNSEYKGTVSVSYDVKLKAHYPNLLDGPYWLGKVPLGENIAD
                     PTVVFPHKTSEYMVYGRRNTENYIADQTYTISMKATKLTVQSFAVYIAAGRVKVGDMK
                     PTEGLANTWELTFTVTKQHIDSGVTNYLEIYQYPSATKGAVQIEWLKLEKGNTRTPNI
                     SEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINS
                     QLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKL
                     YREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSI
                     GADGAIKWRRNSSYGSDYTFAITYTI"
     gene            26772..26921
                     /locus_tag="ACG808_000830"
     CDS             26772..26921
                     /locus_tag="ACG808_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046479.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            26918..27364
                     /locus_tag="ACG808_000831"
     CDS             26918..27364
                     /locus_tag="ACG808_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046480.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            27366..27503
                     /locus_tag="ACG808_000832"
     CDS             27366..27503
                     /locus_tag="ACG808_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFS5046481.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            27541..27834
                     /locus_tag="ACG808_000833"
     CDS             27541..27834
                     /locus_tag="ACG808_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046482.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            27831..28055
                     /locus_tag="ACG808_000834"
     CDS             27831..28055
                     /locus_tag="ACG808_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFS5046483.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            28052..29077
                     /locus_tag="ACG808_000835"
     CDS             28052..29077
                     /locus_tag="ACG808_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFS5046484.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
     gene            29267..29340
                     /locus_tag="ACG808_000836"
     tRNA            29267..29340
                     /locus_tag="ACG808_000836"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29301..29303,aa:Ile,seq:cat)
BASE COUNT        10687 a         4584 c         6313 g         8435 t
ORIGIN      
        1 tataaatcat atctgcagcg aagtaagaag cattaagttc aacttctagt tgagggcgca
       61 ttccttttat ttttatccct gttaaaaacg tactcaatat aaaacttatt attgacatcc
      121 attgaaaaat tgttaagtga aaaaaattca aaataaacac caccagtttt taactaaatt
      181 ataccaaaaa ggagagaaga aataatgcaa gaattagtaa ttttgaaaaa taaagaagct
      241 gtgactacga gcttacaagt ggcagaaagc ttcgagaaaa aacatcaaca tgttttaaga
      301 gatattgatg cactaaaaaa agatgtgtcc aattttggac agatgtttgt agaaggtaat
      361 gaaccagatt catatggcag aaatcgacga gttttcttca ttagtagaga tggatttttc
      421 ttgctggcta tggggtttac aggaaagaaa gctatctact tcaaacaaaa atacattgaa
      481 gcattcaacg aaatggaaga tgttattcgc aagaatactg ttcctcaaac aattgaagat
      541 atgatgatct atcaattaga agaaatgaaa gatgttaaaa aagatgtttc catgcttaaa
      601 gatactatgc gaattagcgg acaacaagag tttgaaatta agcaaaaagg aaatatgaaa
      661 gttatgggag ttctaggggg aaaagaaagc cgagcttatg aagaaatcag caaaaaagta
      721 ttctcaaaat tttggtctga atttaaacgt accttttcaa tcccaagata tggcgagtta
      781 cctcgtaaga gattcgatga tgctgtttca tttattgaaa tgtggttgcc agaaactgcg
      841 atccgtatgg aaattgatca actgaacaga caacagagac tttttggtga tgacaatgaa
      901 tagagctgaa gcgctaagaa tagggacggc aattgctaat tgctggtgga aatactataa
      961 accaatcatc ctaagccaac aacatattga caagcaaaaa gcatggcaac aaataaaaaa
     1021 gtgactccgc cggcaagcaa agagtcacaa agaaaacaca tcataaggag attttagcat
     1081 atggaaaaag aactttccac tctagatcaa tatttgattg atcctgattg gggcaagccg
     1141 aaaattgagg aaacaagtgg tcgaaaaatc agacgaaatc ttttgacgaa tgaagaacta
     1201 gcttgggatc aagatgattt aggcaaccat gtaactattt gggatcatgt ttatcttatc
     1261 catctatcga agcattcgaa taaacctgaa tatatttacg tcatcgaaga tggcttgatt
     1321 gatgcgctag aagagtacga cagagataac ttgattgata tctcttatta cggaccaggt
     1381 aagaaataca ttgctgaaat ggaggcagaa tttgatgagt gaaatcaaag ggacaacgaa
     1441 ctttgaaaaa ctttttagtc gtaagttaaa taaaattctc aagaaaaaag gaaattttga
     1501 ttatttatct tgggctcacg cgtgggagat tatgaaaaag aatgatccac aggcaacggt
     1561 aactattaat gagtacaaac actacagggt tgtttctgga actcatcaag actttcttgt
     1621 tgaggaatat aaaccttttc ttatggatga aactgggact tatgtatctg tctcagtaac
     1681 ggttaaagga cacacggaaa ccgaattatt tcctgtttta gattatcgaa accaaccagt
     1741 tgttaaacca aatgcaatgc aaatcaataa ctcattgaag cgatgctttg tgaaagcatt
     1801 ggctctacac ggactgggat tatatgtatt tcaaggggaa gatattccaa caccacctag
     1861 aatcgataca aagaaattaa acatgctaga gacgattcta gaagctttca atgagcagat
     1921 gggtaaagat atgaccaaaa ccttaatcga atatgttaat gagcagacag ataaattagg
     1981 gctcttagct gataacgttg aaactattga acagttaagc tatgagcaat gtgccttgat
     2041 ggagcgagca atagcagcta agagaaaaga attagataag aagtgatatg agtgtttaaa
     2101 ccattaatcg attcatattc agcggttctg aaaaagttca aaggaaaaga cataagcgca
     2161 accatcaatg aggaagtgaa cattgatcga ctaaagacga tgtatgacgg ctacgatggt
     2221 gatcgagtca ttgaaattcg ttttattgat cctagacgtt tcaccgtaca gcaacgaaac
     2281 ttcatctatg cgctgatagg cgatattttt atcgatacag gcatgccaac ggacttctgg
     2341 aaggaattct tctactttcg ttttgaaggt gtcacagggc gcaaaataag cctgaaagac
     2401 gaatcgaata caactgtgag tgatgccaat gtcttagcaa atatcatctt agatttcatc
     2461 tttgaacatc atattccttt caaagaaggc tatgagattt tacctgcgaa tcaagaatat
     2521 tacttctaca aatgcatcac aaaaagagtc tgctgcatct gtggcaaaac aggagctgac
     2581 atcgatcact ttgacaaagc gctaggaaga cgaaagcgca aagaagttga tcatgcagag
     2641 tacacatttg cagcactctg cagaatccat cacacagaga agcacaaaat aggtgtgatt
     2701 aatttcaaaa ataaatatca aatcaaaggg atcaaattaa accagaaaac aatcaaaaag
     2761 ttaaatattg gagggtaaaa atgacagaac atcgaagtta ttatgcgatt ataccagcca
     2821 acgtaaggta cgacaaaaga cttaaaccaa atactaagtt gttatacgga gagataacgg
     2881 ccttgtgtaa tgaaagaggc ttttgttggg caggcaatga gtactttgca gatttatatg
     2941 gtgtgaataa agagaccata tcgcgatggg taagtgattt gattaagttt ggatacttga
     3001 atcgggaaat catttacaaa gagggtacca atcaaataat caataggtac ctacgaatta
     3061 atcaataccc tattgacgaa aaacgcaata cccctattga cgaaaaagtc aaagataata
     3121 atacatctat taataataca tttaataata caaaagaata tataagagag ttaccgcctt
     3181 cgaaaaaatc gaaggctaag cccgtccgtc ataaatacgg agagtataaa aatgttcttt
     3241 tgtcagatga gcagatggag aaactcaaaa cagaattccc taatgattac caagagcgaa
     3301 tcgaacgact gtcagagtat tgtgaatcat ctggtaagac ttataaaaac tatttggcaa
     3361 ctattcgaag ttgggcaagg aaagaaaaaa gtgaacctaa gaacgcaagc agtggataca
     3421 agcgcacagg aagacgagag aagcttcctg aatgggcaat cgaccaagaa gcctatctca
     3481 agaaaaaagc gctagaacga gctaatagac aatcaaaagc accattctaa gaggtggaaa
     3541 aatgaagatc gattatctag aactaattaa tgaaatagcg aattataaaa agggcgagga
     3601 attagacgtc ctgagagacg tatatgatca actcgaagaa gctggaattg aaggaattaa
     3661 gaatgatcgt tcgagttgga gtaagctcag atactatttc gcactttata tcgatacaac
     3721 acaattaaga aatttagcat ataccaaatt actatttgtt gattgtgtta aaggcttgca
     3781 aaaacatctt aatgaacttg agcaggtgta atcagatgga cctaaagaca tttacagcac
     3841 agattgaact aatgcatcaa gaagctttaa gacaaagtgt atcgtacgaa gacaagtggc
     3901 tcaacacgtt ccatggcgga cgtgagagcg cacttgatca agtactcaaa ttactgaaag
     3961 gagaatgtcg ggatggataa gaaagcggca atgcagcgaa ttatcgaatt gacttattca
     4021 gaagattggc aaaatgacaa agaagctgct tcagaagtga tgaggcttgg aagagcgatg
     4081 tgggcagaca agagcaacaa gccaagacca cgaaaaatcg caatttggca cggtgacaaa
     4141 cttctagtga tagggacagc tgaacagtta gcaagtctca caggcttgca cgagaaaatc
     4201 gtgaggaaaa gagcaaggtg tggctacaca gacgctaaga agagaacgtt tagatacgtg
     4261 gaggaatcat catgacaaca gaagaagtga ttcaaatgcg tattcgaagc cttcagcgtg
     4321 agattgacga tctggaacga acaaaggcag tgatggtcaa tgaaacggcg agaaaggcaa
     4381 tcgatttgca catagagaat ttaagaaggg aaatccatcg attggaggaa tgagcgtgga
     4441 taagaaagca gcaatgaaaa gaattgctga attaaccaag tcagaatctt ggcaagaaga
     4501 caaagaaata gttgcagaag tccaaaagct cggtaaatca atgtggactg aaaagcccaa
     4561 acggagaacg ccgagaaaaa ttgcaatctg gcatgatgac cgaattttag taacaggtac
     4621 tgctgaacag ttatctgaaa ttactggatt aagcaaaaac attatctggg atagagctag
     4681 gagcttatgg attgattcaa aaggacgaca gtttaggtat gtggaggaga gataatggat
     4741 ctcattacac aatacagtga catcatcctc aagaaaatca tgatgaagat tcagaaagat
     4801 aaaaaatcaa aagaacgagc tgaattagtt aagttagaaa tggctgaaac aggagcagga
     4861 gtgcgaagta gcaggcattg gaaagcagca gcaaacattg aattttatta caacgaaatt
     4921 caaaaagggt tcgatcagat gcgtgagctg gatcggcaaa caaattggag caagaaactt
     4981 catcaagatc gtttcaaatt tgtagagaag tataaagaaa tattagaaga gtatttgagg
     5041 aggacagcaa atgataaaaa aactcgttca attcagcatg gatttatatg atatcgaatc
     5101 aggagcaaca ctatctgtgg aatcggacca tctaatcata aattttggtg gaaagcgcca
     5161 gattattttg tgggtagttg atgatgtact gtttccagaa attgttcatg atttcgaaga
     5221 atcaaaagcg gttgagtttg aaatagtgaa aaaagtaatg gaattgattg aaaaatacga
     5281 ggaggacagc gaatgatacc gaagtttaga gcgtgggata aacgaaagaa cgtaatgaga
     5341 gatgtagccg tcttgcattt tactaaaaac ggcaaaacaa actttattga atattggata
     5401 aatcctaccg aattgaaatc atatcacgtg cgaaacatcg acctcatgca atccacagga
     5461 atgaaagata agaatggtgt ggatatattt gaaggggata tagtattagt cagcgtgcga
     5521 aatggcttcg attacttaga taataaagtc tgtattgtca aaaattcaat agattattcc
     5581 ggattagttt gtgccactgt tgatgaagac ttagagtatc aaatttttaa cacagagctg
     5641 tttgaagaat acacgtacga agtcaccgga aatatatacg agaatagcga gttattggag
     5701 gaacagcgat gaataaacag gaagcaatca aaaagttaga aagtattaaa gcgataggaa
     5761 atgatgcaat agctgcttgc tataacgaga gtataaattc aggtattacg ttaatgaaaa
     5821 aaatagacga accgcagaaa ccagttattc cacaactcgt ggccggttgg cttgagaaat
     5881 ctacggaccc ttttacaaaa gctgaaaaaa tagcgtattt aatcaaatct aaagatggtg
     5941 attcatatta tttctgtgat tggtttgtac gagatggcat agtgacgcaa gagcaaggag
     6001 aagaattact cgcttgggca acaagacaat catatgaaac actattgagc ctatacaacg
     6061 gctacgaggt tgagaaagag ccgttatggg caataaagaa tgccgatgga aactatctta
     6121 ctaaatgtgc tttatgggga aaagatggag taaattatag ttttgaatgc aatccatctc
     6181 atcgattgct tttcactgat aaagcaacag cggatgctgc agcattgttg gtgaatggaa
     6241 cagtggaaga ggtggtagaa agatgaaact aaaagacgga ttttacgcta gcagtcacgg
     6301 tatcggcggt ttaatgctag atatgccgac aaagaaccct aaaacacgca agaaatcaaa
     6361 attcaaagtc ggtgacatgg tccgctgtga agcagaagag ttcgtttatc cgttcagagg
     6421 atacgttaaa aagatactgt caaactcagc aatcattcgt attgaaaata caatgaagtg
     6481 tgatcgttcg actgcaaaac acaagcatta tttagctgta gcgagattag ttgacatgga
     6541 agtaatcaag agcaaataaa aaaagccgga tcgctccgac tgattcaata aatccaacac
     6601 atttattata tcacataaag gagcggtttg acttgatgca attgttacga gaggtagatt
     6661 tcaaacagac aagatgtaat gcgagagatg tgctgaagaa ctttcggcgt ttggagcgga
     6721 tggcaggtcg ctctttgata gatattaagt cgccgattat aacggatatg ccgaaggcac
     6781 cgaagcatgg caataaggca gaagacgcga ttattcagat gatggatata gaaacggaga
     6841 gagatgcgat tctagcggct ttgatggctc ttagtctgat tagtcgtcag atactctact
     6901 acagcttctg tgtgccagat agcttctcaa actacagaat tagccgtgaa gtgggttatt
     6961 cagaaagaag tatacaacgg atgaagtcgg aagctctaat agagtttgca gaagcatata
     7021 aacacggaaa aataattgct tataaataat ttggcggttt tttggcggaa tgatggcggt
     7081 ttttagacat ttaccagtgt tattatggta gtgtcgaaag attaggaaac aggatcgaca
     7141 aaataaacga agggaggcaa tctccctcat cgttttaaac ttcttcttag atagacagca
     7201 actaattatg aagaaaagga tgtgaattca actcctccta aattcttcat cggttgctgt
     7261 ttattttgat tagacagcag cactcacaaa actaaacatg ttcttgtcgt ctttcaactg
     7321 ctgctgtcta ttaatttatg tattggaggg aagaaaaatg aaattatcag tagaaggcac
     7381 tccagaagaa atacaagaat tgctccaagc tattggtagt agcaaggagc aaaaggtaag
     7441 tgtttctaat tcagatattg acggtatttg caattgttta aacagagaaa aaatatctaa
     7501 tccttactaa gagaaaatat gttcgttaat gaatttccaa ttttcctctg tagctctacc
     7561 ttgataattt ttttgcagtt cacagatgaa taaggaatcg ttattatcaa gtactgtatt
     7621 taatttttca cacatttgat ttggattcaa attagtttta actaaccaaa aagatttttg
     7681 tagctttata tatgccccaa attcttttat tatttcaaat actttatcat acttttgccc
     7741 tgggttatta agatcataac taatcatgta gggtttatac atgttgtctt gcacatcctt
     7801 gttttatatt tcagcggacc actcgctgat aaataaaatt atacgcttag tatttatttt
     7861 cacaatatta atttatcgta ataacttttg agtaaataga acaaaaaacc tgcactagtt
     7921 tccgctagta caggcagtga ctatatcggt tgataatcta gcatatatta aaaataattg
     7981 caagaaaaga aatttatagt ggtttatacc aaattatcaa agcaatgtta ttttgttgct
     8041 gtctattgtt ttttaattat tcacacgata actaaaggtg ggtgaagaga aatgattcca
     8101 ttaataattt caatttttgc gctctgtctt aatgtctata tgattggatt taaaaatggg
     8161 caaaataaaa aatagtagca gccaagaata attttatagt gtcactgtgg cggaaagggt
     8221 agacgctaag catgtgtgct aggtcaatgc ttcggcaacc atgcaaggtt cgattccttg
     8281 ccagcgacat taaatgccta tgacggttac gactaccgaa aaaagatcgt taagaagcta
     8341 tacggtgcta cgtacggcaa tgtagtaagt gtgctatctg tacaccacca agcttcggtc
     8401 actgtggcgg aagtagaaga cgcagcggta aatggcgagt agcctcgtga gagcctggta
     8461 agttctcgtg agtggtgcaa tcccactcca gcgactttaa gcaaccgagg catcggcggt
     8521 ttaaaaatat aggggtgcgc aatttcgtac gcgttttgtg catcgtgcaa gttactatta
     8581 catattagat cactctttga gtggtctttt tatttttgca caaaggagga aacaacaatg
     8641 tatagaccac aatacttaga acagaagtat gaagtaatca ctgttcataa aggtaatggc
     8701 gaaaaagtat atgagtatag aagaccaata aagagcgata catataaacg aaaggaaaac
     8761 aatgaagtta ttccattgta tggcaaaaga atagctaagc attaaataag attgcgaaag
     8821 gagacggaac atgaccgagg aattctatag atggctatta cagttgacaa gagaagatcg
     8881 tttggttaag ttctatcagt ctcctaaatg gcgcaggctt agagagaaag cgatgaaacg
     8941 agatcactat gaatgccaag agtgtagaag actaggtaag tatcatagag tagagaacgt
     9001 tcatcatata aaggaagtca aggatagacc tgacttagct ttagatttag ataatcttat
     9061 ttgtttatgt gttgaacatc ataatgaagt tcatggcaga tatcttacag cattagataa
     9121 acaagagaag aagatagaaa gctttgctaa cttcgatgca agtgaaaggt ggtaagtgca
     9181 tgatcatcaa tgataatggc agagagtatg atacagaaaa gattgaagag tattcatctt
     9241 atactcaggg attaattaaa cgtttgatat acgttcgcta tgtaggtatt agggatctgt
     9301 tatcagataa ctgttgtagt aaatacaaag tgaatcaagt aagagaagcg ttgaataaag
     9361 ataataacgt cgaaagaata aaaaatgttt ttggatatag tattgaagag attaattatt
     9421 acattgactt cgctgaagct ttcattccga tggtgagata accccccctt aaaataaatc
     9481 gcaaattttt tgggggtgat gaaacggagg gggctgtcag gaaaagagat tttttcgaac
     9541 tttatcatga aaggagggct aaaatgttta aaaacgaatt gtctcaaaat cgctacagag
     9601 aaaaattacg ccgctcttta ataagccaat tggaaagtca gaaaataaat attgagccat
     9661 tcttagataa tgttgatcgt tatatcagtt tatgggaaac ggcgatatca ctggaagaag
     9721 atatatccga gaacggcatt agactggaga atggtaaaaa gaatgaatca gtagcgttgc
     9781 ttgtttctgt caacaaacaa atgggattga tgttggataa acttgccatt actcctgaat
     9841 tggtaggtga agcaaatgaa tcaattcctg agttataagc atattgaaaa ttggttcaaa
     9901 gctatagaag aaggcactat caaggtatgc aaagagcaat tattgctaaa aaattatcta
     9961 gaagaaagag tctttactag agaagatatt tacttcgata agcagatggt agaggattca
    10021 atcaatatac cagcacaata ctttccattc gaattaattc cgtgggaaaa atttctacaa
    10081 tgttttattt atggtgttcg atggaaaaaa gataaaacac tagtgttcaa tagatatctt
    10141 tcattaatgg gacgtggtaa tggtaaaact ggttttgctt cttggaacaa cttctttcta
    10201 ctaaccgcta aacacggtat taaaaattat gatattgata tctatgccaa taatgaaagc
    10261 caagcaaaga ctagttttga tgatgtattt aaagtaatta aagatcatcc tgatttagat
    10321 aaaaaagtat ttaaagctac gaaggaagtt attcaaaata tcgctacaaa cagcaaactt
    10381 cgttataaca cggcaaatgc tagaacaaaa gatgggaagc gaccaggtgc aaaccgcttt
    10441 gatgaaattc acgaaaatga agattattca atgataaatg tggctacttc tggtggtggt
    10501 aaaattcgag attatagaga attttatgat acaactaatg gtcatgttcg tggtggtccg
    10561 cttgatgaca ttatagaaga atcaaaaatg attctttctg gagaacttgg aattgacaag
    10621 gatggagcag aattttctag tttgtttcca tttatttgtc gcttggataa cgataatgaa
    10681 gttgatgatc ccgacatgtg ggaaaaagct tgtccaacta ttaattacaa tgcagattta
    10741 aaacggaaaa tgtttcaaga atactctcaa atgcaacgta atgctggttt aagacttacg
    10801 ttcatgacca aacgaatgaa cagacctatg gaagatacac gatttgctgt tgcttcatat
    10861 gatgatgttc tgcatacgaa agaaaaagaa tttcctgaaa aaatggatga agtgatagga
    10921 acagtcgatt ttgctgatag acgagatttt gccagcgttg ggttgctagg aaaatacgat
    10981 aaagatgttt attttacaca acatactttt atccacgaat cagcccttcg attacaaaac
    11041 atcaaacgag aggttataga tatttctata gatcaaggaa aatcacagat cgttcatgga
    11101 aaaaatatag aagctgatta tattgtaggt tggtttcttg aaatgagtaa taaatattat
    11161 attaaaaaaa tcgctatgga tatgtaccgt gcaaaaatat tgaagcccgc tttagaagaa
    11221 gcaggtttta ctgtggaaat tgttcgaagc ggatctgtta cacatggtat gttaaaagat
    11281 ctggttgatg acctttttat taatcaacgt ttattttttg gtgacgatgc gattatgcgt
    11341 tggtattgca tgaatgtata tgaagagcat atttctaatg gaaatatacg ctatgaaaaa
    11401 atagaacctg aaactagaaa aacggatggc tttttttcat tccttcatgg tttgaatttt
    11461 ttagatgata tttatgattc tgctcctgta acagtcacaa atagctcagt agaaaataca
    11521 ggaactggat ttactcctct agtattctaa cttgaaagga ggtgagaaag tggggatttt
    11581 tcaaaaggcg gtaggatact tcacaaaaaa agcaacggtt cctttagaag aatacttttg
    11641 taaattgcaa gttgattttg tgtatcgaaa atttgcgatt gaaacttgta ttgatttgat
    11701 tgcaaatgcg atgagtaaag cggaattcaa gtcatatgaa gatggaaaaa ataaaaagaa
    11761 tgatctttac tataggctga atgtagctcc taataagaaa aataatgcaa cagaatttag
    11821 aaaaaaactg atcaggagat taatattcta caatgaagta ttgatcgttt ctccgtctaa
    11881 taattctagc gaaatattta ttgcggatag ttgggatgtc acagaatatg cattgaaaga
    11941 tgatgtgttt tctcaagtgc aaattaacaa catagtcctt gatagagaat ttctagaaag
    12001 tgatgttatc tatataaaat acgcagatca acaaattagg caactagtcg atgcgtatta
    12061 tcaagcgtat gggaaactca tttctagtgc catgaatgtt tacaagcgct ctaacgctcg
    12121 tagatacgta ctgaaaggga atttattccg accgcaagac aatacaacac aagatcaaat
    12181 caataaaatg atgacatcac aatttaaggc ttttatggaa gctgataatg caggtgcggt
    12241 atttcaatta caaaatgagt acacattaga agatttcagc ggaaacttcc aaagcaattc
    12301 aagagatata aaaaacttaa tagacgacat ctttgagatg acagcagcag cgtttcacgt
    12361 tccgaaaaac ctactaaagg gagacatgag tgggttatcg gatcaagtgg acgctttttt
    12421 aatgttcgaa atcataccga ttgctgaact tattcaggat gcgtttaacg ctagtctcta
    12481 tgaagtagaa gaatacttgt cagggaattt tgtacgtgtg gatacaacta tgatcaagat
    12541 tactagcttc aaagatttgg ttgacgctat tgatgtaggc attagaaatg gagtatttac
    12601 aatcaacgaa ggaagagaac gcgttggaaa tgatcgctct gataaggcga tggcagatga
    12661 aatatttata actaaaaaca atcaacaagt atcgaaagga ggtgaggcga atgacgacaa
    12721 tgaaaacatt tctagcagta aagaatgaag gcgcagtacc gcaaattttt attcagggat
    12781 ttattggttc tagttggttc tttgaaggga atactgacaa gggaatcaaa aatattttgg
    12841 atagtctagg tgatcaagaa gaaattgaag tagtaattaa ttcaaacggt ggagacgtat
    12901 ttcaagggat tgctattggg aacttactta agtcaaataa agcaaaagtt aacgttgtga
    12961 ttaacggctt agccgctagt gctgcttcaa ttatcgcaat ggctggcgat actataaaaa
    13021 tctacaacaa tgcacaattg atgattcacc gcgcttccac atacggagaa ggaaatgtcg
    13081 atgacttccg tacgattgct gaccaactgg aatcaattga taaatcggta aaggcttcat
    13141 ataaaacacg attcaatggc acagatgaag cattgcaaga acttcttgaa aaagaatcgt
    13201 ttatggatgc agaaacagct ttgagttatg gattggtcga tgaaattatc gatgcagaaa
    13261 atagctcagg tactgaagct aaaaaagaac aaagcgttga agaaattttg aatgacgtta
    13321 aagaaaaaag agcagaaaaa attgctgcat ttacagcagc attaaataaa acatttggac
    13381 aaggagatgt aaaataatga cagttaaaaa tttaaaaggt gtaacagctg caagcgacca
    13441 attgatgaaa gcttttaaag atggtaacga agaatctttt agcgcagcta tggtaagctt
    13501 atctaaggaa attcaggata aaattttaga agaagcaaca gcaaaaaatc aagatcaatt
    13561 agtattaatg aaccgtggtc agcgtgtatt aactacacaa gaaacaaaat tctataacga
    13621 agtagtgaaa aacgaaggtt ttgcaggggt cgaagaatta gtgccagcta ctgtatttga
    13681 acgtgtattt gaagatttag aacaatctca tccactattg caaaaaatta cttttgttaa
    13741 cacaactggt gtaacagaat ggattgtgtc acgtggagtc aatccagcat ggtggggtaa
    13801 actttgcgaa gctgttaaaa aagttttaga taatggcttt gatgtaatta acatgaagca
    13861 gttcaagcta tcaggttata ttcctgtatg taaggcaatg cttgatttag gtccagtatg
    13921 gttagatcgt tatgtccgta ctgttttagt agaatcattg agaattgcat tagaacaagc
    13981 aattgttgat ggtactggta aagatatgcc agtcggaatg atgcgtgaca tgagcaaaca
    14041 aactagcgga gaatatgctg aaaaaaaagc agaacctatt acagctttag atgctgtaac
    14101 tatgggcggt ttgatggcgc gactatcaaa attcaatatc gaaggtgtga atgatccgat
    14161 ttatcgtaat gtgaatcctt ctgatgtggt cctaattgtg aatccaactg attactggtc
    14221 taaagtattc ccagctaaga ctgtactaac tgctaatgga gaatacgtac aagtattgcc
    14281 agtaccagtt tcagatttgc agtcaacggc tgtgccagaa ggaaaagcag ttattggggt
    14341 agcctcagat tacttcatgg gtgtaggatc tacactaaaa attgaagctt cagatgaata
    14401 ccattttgtt gaagacgaac gcatttatct agctaaacaa tatgcaaacg gtcaacctaa
    14461 acgtaacgat agtttcattg tattagatat tagcgctttg ggaactacta ctacaactac
    14521 aaaaccaaca accacaacaa ctacaacaca agcgtaggtg atcagaatga agtatattct
    14581 ttgtcagccg gcaatcaatc ggtttaaatg ggagcttgaa gtttgtttaa ctaatctgaa
    14641 gaaactagga atcaaagata tcgtattgct tttcagcaga cacgatgatc agattcctat
    14701 tttttttgag aaggaatatg gtgttgaagt tcatgtgtac gatgatctgc gggacgacaa
    14761 agagtatatt ccttcgatta aaccatattt atggtggaaa tatttagaag aagatcattc
    14821 gcgtgaggac gaccgatatt tctatatcga ttcggatgtc attttcaata aaagaattaa
    14881 tttgcgcaaa ttgccttcta aagatgatgt ttggtattgt agcgactgct gtagttatct
    14941 aagtcttgat tatattagaa gctgtgaaaa cggagaaaat attctaaaag atatggcaaa
    15001 cattgtaaat gttacagtag aatctttgga aactataaac actaattcag gaggcgcaca
    15061 gtgggttatt aaccgtccta aagcgaatta ttggaaaaag gtttatctgg attctaatcg
    15121 gctatatcgc taccttagag ggcaaaaaac aaatatacaa atctggacag ccgagatgtg
    15181 ggcacagctt tggaacatga tgtatttcaa tattggtcct aaagttcacg aggaattaga
    15241 cttttgtttt gctactgatc caatagaaaa agttaaagaa gtaaaaatct tacacaatgc
    15301 tggagtaaca acaaacgatg aagatttatt tttcaaaggg agatacgtga cttctacgcc
    15361 ttttgatgaa gatttatcat ttgtaaacaa gaaaaaatgc tcttacgcat atgttaaagc
    15421 aattaaggcg gtggttagat gacgcctgaa caagtgactg aagaattgct aatagctgtg
    15481 aaggataata tttacgttac ctggaacgaa gaagatgagt caattaaaaa gatgatagct
    15541 aaaaatgctg tttatcttca aagtaaagtg agtacaacac tttctttttc tcctgaaagc
    15601 ttagaatacg gattgctaat cgaaagatgt agatacgact ggaatcgtgc tttagatgag
    15661 tttgaacaaa atttcgctag tgagttatta ggtttcattc aacattatgc gctacaagaa
    15721 tatattgcag gtgatgtgaa tggcgaataa tcgtagactc gaagaaacat tcaacgatgg
    15781 ttggttaaag attttgacgc aaactaccaa aagaaatgaa ctaggaaaaa agattggtgt
    15841 agaagataca gaaatcactt ctttaaaatt tagaaatctt tccatgagag atagtgatat
    15901 aacagctatg gatgcgatgg gatcgaaatt aactaagaaa gtaaagactc catttcatcc
    15961 aatcgccaag aaatttaata aagatcaata ttttatcgta atcgatagta tgcgttacaa
    16021 cgttatctat gccgattacg ataattttta tatctatttt tatcttgaaa gtgtgggtga
    16081 atatggtgat tgataattct aaagaaaaag aacgtttaaa taagcaaatt tctgctatca
    16141 aaacttcctt agaagagcat tttggcctca aactctttca agactccgta ggcgaggatg
    16201 agctacctga tgattttaat tacttcattc tcgaaacagg agaaatagaa atgatcactg
    16261 agccaaaata tagcgtgggt caaaatctat atctaacttt ctattcagaa aatagagaag
    16321 atttaacagg agactcacta gatattattt cattgattca aaatcgttcg attcgttttc
    16381 agagaatgga tcccaatcat ttaaaactag agaaccaaga tcgctatatc gatcaattgg
    16441 tatttacgtt tagacgatta ttgaagagtg attgtcatgg ctaaaaatag ttgggagcta
    16501 aaaataaatg gacatgatga acttcttgtg cggatggaac gctattcaag cgagagcgaa
    16561 cgactgatta acgaagcatt gaaatcaaaa ggttcggcta ttgcagtgga taggattaca
    16621 gaaaaaattc ctgtttctga agcagattta agaagagggc accaacacgc aaaaaatagt
    16681 cgtccactta agactcaata cattaatttg ggtttcatca ttagacctac aagaaaattt
    16741 gagtatttaa aatatcctga tttggggata ggtacttcta aaagaaatca gccggacgaa
    16801 tttatgagaa gaggattagg tcttgcactt gatccaatta cagaacttct gattcgtcaa
    16861 ttcgataaat taaataaata gggggaacaa caatggctaa aacaaaaact gtagtaacaa
    16921 cgttcgataa cgtaagtatc aaacgaattg cttttaattt taagaacgca ggaaatgcaa
    16981 tcgcaacgaa ttgtaatgga caattagatg gcgaaacaga aatgcaaacg gtggttaaaa
    17041 aatgtggagc gacagaagta aaatcaaaat ctaaaccaat caatatgacg gtaacaatta
    17101 ctgcacatgt accgatggaa gtttatcgac gtttcaatgg gttgaaacaa gatggacgta
    17161 ttaaaccagg catttactct tacggtcctg attccgtagg cgaagatttc tcacttgctg
    17221 cagagatcgt ggatgacttc gaagaaaata gaaagttagt tggtatgtta gcatgcactt
    17281 cgaatacagg attaacattc tctattgaaa atggtgcgga tgaagtagct gcgttagaac
    17341 tagaaacaaa agttatgcaa gatgaatttg gtaaatttta tcatgaagca attgttgcag
    17401 aacttgaaga agacttaaca gatcaatgga tgacgaatct atctgctgat gtgattaaaa
    17461 aaacttcagt tgtgacaact acggccactc aatcacagta aaaaaaacgg aggtagcgaa
    17521 atgaacgaag attactcaaa aattgaacta aacgatggaa caattttgaa tttagaacct
    17581 aaactgaata tcaagaaatt attgatgatc aatagagatt ttaacacaga cgagtttgca
    17641 aaaatgactg tgggaaaagg atccatggat atttctgtta ttcaaggtgc aaaggctgtg
    17701 tatattgctt accgccaagc gaacatgact gattatattt cattcgatga atttatcgat
    17761 aaatgggatt ttgatatggc tactgccagc tatatttatc aattgatgat gttcaaacaa
    17821 gcacgcgatg cttatcaaaa agaatttgaa aaagcaaata aggaaaaaaa gcttcaaaag
    17881 taaaaattcc aaagctctta gttgaaacgt gggtcgatgt ctattcgatg ttgaccgacg
    17941 ttttttctat gccttcagat ttggttttaa gcgatatctg tttagatgac attttgcaaa
    18001 tggcttacaa caagagtgct tatgaaggat ggaaaaacta tgcaataaac caatcccaga
    18061 aaaactaaag aaaggaggta aaaaatggct aaaaagagaa cagaagcaga agtaactttc
    18121 atagctaacg atgacggatt gaaatctacg ttaaaagaaa tcagtgctga attaactaaa
    18181 aatagagcag aattaaaact agaacaagct caattacaac agactggttc tgaatcagac
    18241 aagttaggaa gtaaattatc ttctttagaa aagcagtatg aattacaaag tcaaaaagtt
    18301 gaagtaacta gccaacgttt agccaatgcg aaaaaatatt atggagaaaa ttccaccgaa
    18361 gttcagaaac ttgaaagaga actgattaat caacaaacag cgcaacaacg tttgtcaaac
    18421 gaaattgata aaacgagtaa tgcactagct caagcaaaag gcgaaataca gacgtacgag
    18481 tctacaatgc aacagttgga tagcgaacaa aaaaatgttc aagctagtgc ttctctgatt
    18541 gaatccgaat acaaaaaatg gcaagcaact gctggtcaat cagcttctga agccgagaaa
    18601 ttagcgaaag cccaagaata tgtttctcaa caatctgaaa atgcggagaa aacgatagat
    18661 atcctaagac gacagttaga agctacacag tctgaatttg gcgctacatc cacagaagca
    18721 atgcagatgg aagcgaagct taatgatgct gaacgtgaat ttgaagagtt aggacaagct
    18781 gctaaaaatg tagatacaac taacttggac gatatcggaa gcaaaataga tatgaataat
    18841 ttaatggaag cttctgacgt tttaagcgac attggcgata agcttacaga attagggaaa
    18901 caagcagtgg actctgctaa cagtgtaggt agttcccaga gtaagataca agctaatttt
    18961 ggtttgtcta aacaagaggc tgaagaatta acgaatgtag ccagagacat ttattataaa
    19021 ggttttggag aatcgttaga tcagtccaca gatgcattga ttttggtaaa gcgtaattta
    19081 ggagatttaa ataatcaaga tttacaaaat atcacggaac aagctatggt cctagaaaac
    19141 accatgggcg ctgatatgga tgaaacgtta cgtggtgtaa atggcttaat ggtcaatttc
    19201 ggcttgagtg ctcaagatgc aatggattta atggtttcgg gtactcaaaa cggtttagat
    19261 aaaacgcacg aattaggcga caatatggca gaatatagcc aattatggag tcaaatggga
    19321 tattcagctg atgaaacgtt cggaatgctt caaaatggtt tagatgcggg tgcttataac
    19381 cttgataaag tcaatgactt agttaaggaa atgggaatat cgttaacaga tggtcgattt
    19441 gagcaaaaca tggatatgtt tagtgaaagt actagaaaag cttttgaaga gtggaaaaat
    19501 ggcggaggaa cacaaaaaga cgttattaat tccatgattc aagattttag caatatggat
    19561 ggtcaatacg accaattaaa taaagcttcg acaatttggt ctgcgcttgg cgaagataat
    19621 gcgatgaaag ttgtccaatc tttaactgat gttaaccata catttgatga tgttagtgga
    19681 tctgcacaaa aaatgaatga agattctact actccgttgc aagagttgaa cgggaaaata
    19741 gctgaattaa aggattcatt agctcctata ggcaacacaa tcatagatgc actcgaacca
    19801 gtaattgatt ttctaggaaa gatggctgat gcgtttaata atcttccaca accagtacag
    19861 gatttcattg ttgctatagg tggtcttaca gcagcatttg gcatactgtt accggcaata
    19921 ctagccgtgt catatttatt tggtccgatg atgcttataa ttggaggaat tatagctgtt
    19981 atagcaggcg ttattgtggc aattaagaac tggggtgcaa ttactgactg gtttagtggt
    20041 ttatggaaaa aatttactga ttggttgggt gatacttggg aaagtataaa agacggagcc
    20101 tcatcagttt gggatggggt taaagaaacc tggtctggat ttgtagattg ggttcaagat
    20161 atttggcaag gagtttctga ttggtttgga gagctatgga gcggattagt tgaaggagct
    20221 tccaacatct ggcaaggagt ccaagaaact tggcaaacat tcgttgattg ggtttcaaat
    20281 atttggaacg gagtcaaaga agtatggtcg attatttggg cagacattgt aggaattgtt
    20341 caaataccat ggacattaat aacgtcattg attcaagctg gtattaatat tatcgtgggt
    20401 atttttgatg tagctggaca gttattgggc gcagcttggc aagctgtttg gacacctatt
    20461 tctgatttcc ttaaaaatac ttgggatact atgacacaat ggataagtat cgcttggaat
    20521 gggattgtaa ctacattcca tactatattt gatccagtag tggcatggtg gaatggtata
    20581 tggacagcta ttagtactac ggcttcaaat atttggaatt caattagtgc aacagcttct
    20641 agtatttgga acagtatcaa gaatacaatc actagcttgg tacaagcagc tgctacagta
    20701 attcaaaata tttggtcaac tgtatctagt tggttaggtg gaatttggaa ttcaatcagc
    20761 tctacagcat caaatatctg gaatagtgtg actagtagta taagcaatgc tataaacgca
    20821 gctaaaagtg ccattcaaag tgtttggaat agtatatctt cgtggattag cggaatttgg
    20881 aacggtatca aaaatactgc tttgaatctt tggaatggaa ttacaagcac tattagctct
    20941 aaagtaaacg atggaaaaaa tgcaatttca agcggttggt ccaatctaac aggtattgtt
    21001 tccgacatat tcaataatgt taaaagtaca attgctaaca tatgggaagg catcaaaaag
    21061 actgttagcg ctccaattga ttggattaga gacaaaatca gtggcatttt tgataatttg
    21121 aatatttcta taccacatat tccgttaccg gaatttatca tggaaggcag ctttaacccg
    21181 ctaaaaggtc aaatccccca tttgcgtgta aaatggcatg ctaaaggagg tatctttact
    21241 aaaccaactt tactaggtgg aatgaacggt gtcggtgaag caggacctga agcagtttta
    21301 cctttgaaaa gatctgtttt gcaagaaatt ggtgatcgta tcttgagtag cacatcagtt
    21361 tcatctaggg cacaaacgat tcaacctgtg aataactacg aattcaattt cacaattgat
    21421 ggtaacgcag atgaggttac tatgaagcaa acaactcaac aaatcattga tagcattaca
    21481 aaaattcaaa atgataatgc ttcggcatgg cgttaaacag gagagtattt ctcctgtttt
    21541 tttagtatta aaaaggatgt gaaaaaatga ctgattgtat acattctata atcgatggat
    21601 ttcctgatta tttgcataaa ttggctttag ctgaaagacc aaccatacct tctccaaaaa
    21661 gacagagagt tgaaacttct gttttaggta ggttaggtgg cttagtacaa gattactcgt
    21721 ttgaagatat gtcgtttaca ttgcactata actatttaga ggatgtggaa gaccatcaag
    21781 cgttcaagca atcgttttat atcatgcgtc attggttaaa ctatgcaaag aaattagaat
    21841 tctctgacga tcccaacgtc tattacgtta tccagactat cgatattggg gatgcagaaa
    21901 acgatattgt tgaatgggga gagttcgatg taaatattac tgcgaaacca ttcgcaagag
    21961 ttcaagaaga tgtacctata accgtagata aaccacagtc atttaacttg ctgaataata
    22021 gtttagaaga aagttttcca aagattatca tcactccttc agctacttca tgccagttca
    22081 tcttaaatga ttatgtgttt agtttcgaag gcttagtagc aggaactgat gtagtcattg
    22141 atagtgattt gatgctttgc tacgaagagc aatcggacgg agatatttta gatcggtcca
    22201 acaaaatgaa gaccatgcaa tatccgacat tgcaagtgga tatcaatcat tttaattgca
    22261 ctggtttgag caaaatacaa atttatcgta acgggttaag gtaggtgaaa tagatgatcg
    22321 ataatttaat aactatttac gataaaaatg acgcgaataa tttagctgaa catttatatg
    22381 atacgcaagg tttaggcgct ttgtcagact ggttaacagc tactgttagc aataaactaa
    22441 acggagccga gatatttcag ggtacttatt caataaacgg aactaatgca gatttgattg
    22501 tagacggacg tattattcag tgttatgtag atgaaaatcg agcaaaacag cgtctacgga
    22561 tttattatgc aaaaacttct gtaataggca atacgataga agtaaaagct gaacctattt
    22621 tcaatgatat aagaaaatcg gtgttgaata aatatgacag cggaacagaa aagatcactg
    22681 ctactcaggc atggcaaaac gcaaaagttt tagcgaaacc agctatccct tcgcagtttt
    22741 ctttctcgtc attagtagat acgcttgcta atgtgaagat agaaaaggcg aattttttag
    22801 aattctttgg tggaaaagag ggatctattc tagatcgatt tcatggtgag tttctaaaag
    22861 ataataacac attacgtcat gaaaaaagtc taggtacgga tcataaaatc aaagcgattt
    22921 atactaaaaa cttaactggt cttgacttag agattgatgc tcaaagtgtt ttagttggag
    22981 tttatccatt cattagcagc tcttcagaag gagaagacga gatcactcta ccagaagaag
    23041 ttattttcac ggattacgtg gatgattatc ctgctggata tgtttctttt gttgatttta
    23101 aagacaaagc gactgatgta gccacattaa gggaagctgc taaagactgg ttgaaaacaa
    23161 acatagataa acaaaaacca caagtgagtg gttcgattga attagtacca ttgaggcatc
    23221 aaagaggcta tgaaaaattt gttgatctag aaaaagtttc gatgggtgac ggagtagatg
    23281 tgtatcatcc acagttaaaa gtgaatatgt cagcgagaat tgtggaatat acgtttaatg
    23341 ttctaaccaa ttcatacgat aaattagttg taggaaacgt caaaacaaac ttcttagaaa
    23401 acacagagaa taatgtcagc aatttgatta atgatgccat tgatcaattg aaaaacggtg
    23461 gcgaaatcag tgatttactc aatgatattg tagatcatca aactgatatg attactggtc
    23521 aaaatggtgg ttatgtttta ttagatccta aagaagcgcc tagtcgtatt ttgattatgg
    23581 acacaccaga taagaatacc gcaaggaatg ttttacaaat caacaatgct ggtattggtt
    23641 tttctaaaac tggcattaat ggaacatatg aaacggcatg gacgttagat ggcggattca
    23701 atgcctcgtt tattacggct ggtgaaatag taggaattac tattagaggt actacattaa
    23761 ttagtgatgg tgctgattat agaacaagta ttgctaatgg caaaatgact tggtactcaa
    23821 aaaaagttaa caaagatatt atggagctag aagcacgtga ttatgtaagt gctgatgccg
    23881 gtattgtatc atacaccatg aaaactggtg gtggtttcat gattagaaat ccacagggta
    23941 acttggtttt tagtacgtgg gataatggta ataacagacc gtttctatct tttggtgcgc
    24001 ccaatttcag gtatagcaat gctagttatg taacttctgg cgacggtagt tctttaagca
    24061 ttaatggtag tgcgggtaac tcatgggaat ttaaggtagc tggtaggact atgaaattta
    24121 ctagtgatgg tatgctaacg ttaccaggtt gtttttttgg ttcatgggaa gatgggaaac
    24181 ttgctaggtt tgaacaatca acggtacaag tatataaaga ttttactgtt agaggtacta
    24241 aaaactcaac tgtaccaaca gaacattatg gacaacgact attgaacgct tatgaaactc
    24301 cagaatatta tttcgctgat tatggggaag ccgttacagg tgacaatggt aaagttcgtg
    24361 ttgatattga ccccatgttt gctgaaacag taaatctaag tcggtatatg acacatgtga
    24421 cacctacaga actagttttg tgtgctgtta ctcatgaaga tattgaccat ttcatcattg
    24481 aaactagtaa gccaaacgta ttagttagat ggaatttagt ggcacaccgt ctagggtatg
    24541 aagatattag attaaaagag gatacagcat atgatagcac agtgcttgac caaaaacgtt
    24601 tttaaaacga agacaaggag gtatataaat ggctagcagt ttatataatt tggctttaga
    24661 tttcagcaaa gaattaaact acaccaaagc tattatggct cgtcaaggtg ataaagggat
    24721 tacggtgacg gttaaaccgt ttctaaatgg cttgcagatg gatacgagtg gcggaacatt
    24781 tactttaaaa ggaacaacac catctaaccg ttacgtagat aatgttgcaa ctagtgtaac
    24841 tagtgaagaa gtcacgtttt ctcttgatgg cacatttatg agtgaagcag gatattataa
    24901 acactgctac gtagaatata gaaaagacaa tcaaatttta acaacgcaag atatcatttt
    24961 tttctcacta ggagtgtctg acatttcgca aggccaagcc gatgaatatg tttcgcaatt
    25021 agaagagttg attcgaaagt acaacgaaac ttttgatgct tttatggctg aaatcaaagg
    25081 tagagtggat agcttaaatc aacagattac tgatttaact ggtcaagcta aaacgctaca
    25141 agacaagtta gatgctctga aagaagaaat ttctaagtta ggtaacttac aagtgatgta
    25201 cagtaacagc atcgacttcg gggactatga ttattcggag aacccgaatt taatgccata
    25261 tattacagaa ccttgggttg gacctttgtt aggtaatgga catacagtta aagatagtgt
    25321 taaaagggtt ataactcata ctaaaactag aacagctaat tccggcgaca tattatcgct
    25381 tggcttaggt attccgtgta cagcggaagc taataatagg tatcttataa ccactttacg
    25441 accatctacg acatacacat taagcgttac tatgagtgtc ggttcagatt ggactggaga
    25501 gaccaatact ataggggtta ggcttagata tttaaatgaa caaggtggta tagaactgcc
    25561 cattaatgca ttaataccag caaatgtaga acgcgataaa atggtcactc atacatttac
    25621 tggtataaca aaagataatg taactagcat aacaaactgt tatgttgaaa tattctcact
    25681 taatagtgaa tataaaggaa cggttagtgt cagctatgac gttaagttaa aagcacacta
    25741 ccctaactta ctagatggcc cttactggtt aggtaaagtt cctttaggcg agaatattgc
    25801 tgaccctaca gttgtctttc cacacaaaac tagtgaatat atggtttatg gtagacgcaa
    25861 tacagaaaac tatatcgctg accaaacata taccatatca atgaaagcaa ctaaactaac
    25921 agttcaatcg tttgccgtgt atattgctgc tggccgcgtt aaagtaggag acatgaagcc
    25981 tactgagggg ttagctaata catgggagtt aacttttact gtaactaagc aacatataga
    26041 tagtggggta actaattatc tggaaatata ccaatacccg tcagctacaa aaggagctgt
    26101 tcaaattgag tggctcaaac tagaaaaagg caatactcga accccgaata ttagtgagta
    26161 taaatatcgt ggtactggta tgcgtgattc aaacaatcca aaagattatg tttgggatct
    26221 agcaccagaa tatgtcgaag ataatttggc cacagatatt aaaatttctg aaattactgg
    26281 taaagcaaac aattataccg atgggaaagt atcggagatt aattcgcagt tgactgcttc
    26341 aattaatgaa gtagacacca cagctaagga tgctcaaaca aaagcgaatg ctaatgcgac
    26401 tgctatagat gaattagaca ataagatcga tgaacgcatt aatgatacag ctactaccac
    26461 attaacagtt acaaacggga ataccggatc agcaaagctt tatcgtgaag gaaaaacagt
    26521 ttctatatat tttgtggctt taaacggaaa aagcagtggt ggaaatgatt caacgatact
    26581 aacaattcca gaaggctatc ggccaccaat tagttttgag caactggttg gctcgataga
    26641 ccgttctact ttgaacagtg ctcagttatc tattggtgca gatggagcca ttaaatggcg
    26701 aagaaactca agttatggat cggattatac ctttgcaatt acttacacga tttagaaaag
    26761 cgtgaatcga tatgaaggca gcatatagac caattgaacc ttacggattc gagcaaatca
    26821 ttgtgaatga tgaagaacat ttaccggaag aatgcacaga agtcgaacca ccgattccaa
    26881 attggaaacc gaaattcaat tactgggagg gaaataaatg aaaaacattt ggaaatatgg
    26941 acgtactggc ggagagtacg caggaaaagt attggacgac atgcttgtat ccgttcctta
    27001 cacagatcag ccaccgcttg aaggaattcg tgctgatggc gaaccgttaa caatcgctga
    27061 tcaaatgttt gatcctaaat tgaatcaatg gattatttta gcgaacgcac tagatcacaa
    27121 cgatttaaac aatctcaaag caatgtatga gtcgttagaa aatgagaacg gcgatttaaa
    27181 acagatcaat gccaaactca tgctaagcga tgtagcaatt aaacaggaaa atactgcatt
    27241 gaaagaaaaa gcggatagtt tagcacaaat caattcaaaa atgatgcttg cttcgttaca
    27301 aaatagcaaa gacatttcag aaattaaaga gcaactaaat ccagcttcaa agggaggtga
    27361 gtagtatgtt tagttttagc gatgtgaaaa tgatgtatga ttggggctgt ttcactaacg
    27421 aacaagtaat ggttttcgtt ccgttgtgca ttactgaaga aaaagcagat aaaatcatta
    27481 gcaaagaaga gagcgcatct taattgatgt gctttttatt ttgattcaag gagttgtcac
    27541 atgattaatt taggggaatg gggagcgata gcaggatcaa taaccgctat cgtttctttg
    27601 attttattag taataaaacc aattactgca tctttctcga agattactga gactctttca
    27661 aaagtaagtc acaatttaga tttgctgact aaagatttag aatcgagcaa atcagatcga
    27721 ttgatgattc atgaagaact aaagaaacac gatgaaagat tagatacaca tgcagaaaaa
    27781 ttggtagaac acacgcaaca aattaaaact ttatttaggg agagaagaaa atgaataata
    27841 aaacgttcga agtactaaaa tggttcgcac tggtaattat tcccgcacta gctactttcg
    27901 tggggttagt tggtaaagcg ctcaattggc agtacacaga tatctgtgtt gtcatcatta
    27961 ctggttttgg cgcgttttta gggagtgtgt tgggtgtatc aaatcgaacc tacaaaatgt
    28021 tctcggctga aagcgaagaa ggaggaaaca aatgaaaaag aaaattacta ttactgcgat
    28081 gagcctgtta acggctcttt ttttattacc cattaatacg tttgcttata ctattaatga
    28141 cgagtataat ttagcgccga atcaaggaga ctccagatta gcaattccta acaaaattat
    28201 tttgcacgaa actggaatag atgcaccagc aagaaacgta gccgccaaca tgaaaaataa
    28261 ttataacgga agcaattctt atactacaga tgttattggt gacggtggga ttgtttaccg
    28321 tgtgggtgag caaggatatg tttcgtgggg agctggtaac gctaatcctt atgcgcctgt
    28381 acagattgaa ttacagcgca catatgataa agcattgttt gaaaaaaact atcgagctta
    28441 tattgaatat acaagagata gtgcaaaaaa atatggaatt ccattgactc ttgatcaagg
    28501 aacttcttta tttacaaaag gaatcatttc tcatttgtgg gtgacaaatt atgtttgggg
    28561 gaaccacaca gatccatatg gttacttatc gcaaatggga gttagcaaag aaaagcttgc
    28621 ttatgattta gctcatggat ttaccgatga aaatccaaca acttctgaaa acaagcctgt
    28681 cattgatcca acacgagctg gtgcagctaa tcctacgctg acagatggaa caaatcacgc
    28741 ccacattgat cagtttgggg aaatcgaaaa cgcaaacttg catgtggctg gatggcacat
    28801 tgctaactat aaatacgagt atattttcat tatggactac aatactggga aagaattagc
    28861 tcgagtaaga gctgatggaa tttatagacc agatgtaaac caagcttata atacttctgg
    28921 aaatgttggt tatcatgtat ctttcaatat gcgtaatttt cctagtaaga aagtctatgt
    28981 aatgatgcgg gcaacgaatg atccagaggg aaacactaaa ggcggtgcgc aagatttcca
    29041 tgacaaacgt tggtatttaa atattcctaa acgataaaaa tagctcctcg ttgaggagca
    29101 gtacataact atattgacaa ctataaaaat tattcgataa aatagtgatg ttatcgcata
    29161 tcttcactat cacccataaa tagtcacact ccaagctatg cgataacagg tttgttgcca
    29221 cacattctac tggttgattg tttatggctt tatgtggcaa caaccagtac ccttagctca
    29281 gttggtcaga gcagacggct cataaccgtc cggtcgtagg ttcgagtcct acagggtaca
    29341 ttaacgtagc catttgaatc gttctgtgtt agaatttttt tgaagagtat tatacaagct
    29401 aaagcttttc ttcattgcca ctcaaatgag tggctttttt atgtatcctt ttatggatta
    29461 atgaaaggat gtttcacata gttatacttc tgtatatttg aaaagtttta ctttgatttt
    29521 taaatagaaa gacatttggg ttaaattgtg agataataat aaagaagagt ttaaagcgca
    29581 ccccaaacca cttccccata agtgtgttac gctttaaact cttttatatt tgaagccatt
    29641 aaaaagcata ccatattttt gaaaaaaagt gagaaaaaag gcttacaatt ggagtggtag
    29701 ttaattagtg acttattttt gattttatag cactgatact ataaaatata gatatcatca
    29761 tattacacaa tcttaatact aacttaaaaa atatctcctt tcacaagtat ggtgataaaa
    29821 ttcgttccgg gctacctttt taggtagcct actttaatct ttataccttt ctggatcaac
    29881 gaaagtatac tttatatagt cataacgccg atgatcgcta cgtgcgtccg gcacgtcagt
    29941 catgaatcgg ctacactaga ctagacagaa aaaataaggt gtgtagaata ataaaaaaca
    30001 cactggagga tttttcatg
//
LOCUS       DAZUMK010000017        29469 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000017 DAZUMK010000000
VERSION     DAZUMK010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.017"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            38..907
                     /locus_tag="ACG808_000837"
     CDS             38..907
                     /locus_tag="ACG808_000837"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFS5046485.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            1072..2343
                     /locus_tag="ACG808_000838"
     CDS             1072..2343
                     /locus_tag="ACG808_000838"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFS5046486.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFIGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            2340..3635
                     /gene="rho"
                     /locus_tag="ACG808_000839"
     CDS             2340..3635
                     /gene="rho"
                     /locus_tag="ACG808_000839"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFS5046487.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            3734..3997
                     /locus_tag="ACG808_000840"
     CDS             3734..3997
                     /locus_tag="ACG808_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFS5046488.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            4442..4891
                     /locus_tag="ACG808_000841"
     CDS             4442..4891
                     /locus_tag="ACG808_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5046489.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            4948..6366
                     /locus_tag="ACG808_000842"
     CDS             4948..6366
                     /locus_tag="ACG808_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFS5046490.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            6366..7226
                     /locus_tag="ACG808_000843"
     CDS             6366..7226
                     /locus_tag="ACG808_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFS5046491.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            7400..8173
                     /locus_tag="ACG808_000844"
     CDS             7400..8173
                     /locus_tag="ACG808_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS5046492.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            8320..8685
                     /locus_tag="ACG808_000845"
     CDS             8320..8685
                     /locus_tag="ACG808_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFS5046493.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(8833..10041)
                     /locus_tag="ACG808_000846"
     CDS             complement(8833..10041)
                     /locus_tag="ACG808_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5046494.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(10060..10338)
                     /locus_tag="ACG808_000847"
     CDS             complement(10060..10338)
                     /locus_tag="ACG808_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046495.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            10531..11187
                     /locus_tag="ACG808_000848"
     CDS             10531..11187
                     /locus_tag="ACG808_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS5046496.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(11629..12426)
                     /locus_tag="ACG808_000849"
     CDS             complement(11629..12426)
                     /locus_tag="ACG808_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFS5046497.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(12423..13607)
                     /locus_tag="ACG808_000850"
     CDS             complement(12423..13607)
                     /locus_tag="ACG808_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFS5046498.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(13632..14879)
                     /locus_tag="ACG808_000851"
     CDS             complement(13632..14879)
                     /locus_tag="ACG808_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFS5046499.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            15094..16839
                     /gene="dnaX"
                     /locus_tag="ACG808_000852"
     CDS             15094..16839
                     /gene="dnaX"
                     /locus_tag="ACG808_000852"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFS5046500.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            16859..17173
                     /locus_tag="ACG808_000853"
     CDS             16859..17173
                     /locus_tag="ACG808_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFS5046501.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            17198..17794
                     /gene="recR"
                     /locus_tag="ACG808_000854"
     CDS             17198..17794
                     /gene="recR"
                     /locus_tag="ACG808_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFS5046502.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            17858..18502
                     /gene="tmk"
                     /locus_tag="ACG808_000855"
     CDS             17858..18502
                     /gene="tmk"
                     /locus_tag="ACG808_000855"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFS5046503.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            18517..18846
                     /locus_tag="ACG808_000856"
     CDS             18517..18846
                     /locus_tag="ACG808_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFS5046504.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            18860..19798
                     /gene="holB"
                     /locus_tag="ACG808_000857"
     CDS             18860..19798
                     /gene="holB"
                     /locus_tag="ACG808_000857"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFS5046505.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            19834..20658
                     /locus_tag="ACG808_000858"
     CDS             19834..20658
                     /locus_tag="ACG808_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFS5046506.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            20651..20998
                     /locus_tag="ACG808_000859"
     CDS             20651..20998
                     /locus_tag="ACG808_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFS5046507.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            21067..21939
                     /gene="rsmI"
                     /locus_tag="ACG808_000860"
     CDS             21067..21939
                     /gene="rsmI"
                     /locus_tag="ACG808_000860"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFS5046508.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            22048..23169
                     /gene="dinB"
                     /locus_tag="ACG808_000861"
     CDS             22048..23169
                     /gene="dinB"
                     /locus_tag="ACG808_000861"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFS5046509.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(23223..23825)
                     /gene="nrdG"
                     /locus_tag="ACG808_000862"
     CDS             complement(23223..23825)
                     /gene="nrdG"
                     /locus_tag="ACG808_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFS5046510.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(24140..26329)
                     /gene="nrdD"
                     /locus_tag="ACG808_000863"
     CDS             complement(24140..26329)
                     /gene="nrdD"
                     /locus_tag="ACG808_000863"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFS5046511.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            26655..27422
                     /locus_tag="ACG808_000864"
     CDS             26655..27422
                     /locus_tag="ACG808_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5046512.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            27412..29454
                     /locus_tag="ACG808_000865"
     CDS             27412..29454
                     /locus_tag="ACG808_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5046513.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT         9440 a         5308 c         6171 g         8550 t
ORIGIN      
        1 caaatttagt tttacaaaac ttaggaggaa ttttattatg ccagtagtat caggagctga
       61 atttttaaaa gctgctcgta aaggcggtta cgctgtcggc ggattcaaca caaacaactt
      121 ggaatggact caagcaatcc ttgaagcggc tgaagcaaaa caagcaccag tacttatcca
      181 aacatcaatg ggtgcagcta aatacatggg tggttacaaa atctgtaaag atttaatcac
      241 tgatttagtt gaagcaatga acatcactgt tccagttgct atccacttag accatggtga
      301 ttacgaagct gctttggaat gtatcgaatt aggctatact tcagtaatgt tcgatggttc
      361 acatctacca tttgaagaaa acttgaaatt agctaaagac gttgttgaaa aagcacatgc
      421 aaaaggtatc tcagttgaat gtgaagttgg ttcaatcggc ggagaagaag acggaatcat
      481 cggtaacggc gaattagctg atattgaaga atgcaaacaa atggttgcta caggtatcga
      541 ctacttagct tgcggtatcg gtaacatcca tggtcaatat ccagaaaact ggaaaggatt
      601 agcttttgac cacttacaag caattgctga tgctgtaggt ccagacatgc ctttagtatt
      661 acatggcgga tcaggtattc ctcaagatca aatcgaaaaa gctatctcaa tgggtatctc
      721 aaaagtaaat gttaacactg aattccaatt atcatttgct gcagctactc gtaaatatat
      781 cgaagctggt aaagataaag aaggtaaagg attcgaccct cgtaaattat tagctccagg
      841 taaagcagca atcgttgaag atgctaaatc tcatatcgac tggttcggtt cagctaacaa
      901 agcgtaagca tccacataac agatacgata tataggaatg gagaacgact tttgaagtcg
      961 ctctccattc ttttttactt aaaacggcag accatgctcg tttctttttt ttatctatga
     1021 taaaatacaa gcatatccat gaaaaaagat taataaacaa ggtggaaaca gatgaaaaaa
     1081 atcgtaatca aaggaaaccg acctttagct ggtgaagtaa cgatcagcgg tgcaaaaaat
     1141 agtgcagtcg cattgattcc agctgcaatc ttagctgatt ccccagtgat cttggaaggt
     1201 gtgccggaca tacaagatgt tcattcatta attgaaattt tagagatcat gggcgcaact
     1261 gtccattttt ccaacaacat actagaaatc gatcctcgag aagtcgtatc gattccgatg
     1321 ccgcacggaa aaatcaatag tttgcgtgct tcttattatt ttattggaac actgcttggt
     1381 aaatttggcg aagcggtagt aggcctgcca ggtggctgtt atttaggccc tagaccgatt
     1441 gacctacacg tcaaaggatt tgaagctcta ggtgctgaag tgacaaatga acatggtgcc
     1501 atgtatttac ggacagaaaa taaaggattg cgtggcaatc ggattttcat ggacgtggtt
     1561 tcagtcggag caacgatcaa tgtgatgtta gctgctgtaa aagcaaaagg acagacagta
     1621 attgaaaacg ctgcacgaga accggaaatc atagatgtgg caactttact gaataatatg
     1681 ggggcaaaag ttcgaggagc gggtacagat gtgatccgta tcgaaggagt cgaaacatta
     1741 catggctgtc ggcatttcat gattcctgac cgcatcgaag caggaaccta tctagcatta
     1801 gcagctgcag taggaaatgg tatcaaagtg aaaaatgtga tctttgaaca tttggaaagc
     1861 tttatagcaa aattgcagga aattggtgtc catatgaaaa tcagcgaaga tgaaatcgaa
     1921 gtttacccct cgaaagaatt aaaacccgca aacatcatga cctatcctta tccaggattt
     1981 gccacagatc tgcagcagcc acttactgcg ttactattga tgactacagg cacttcagaa
     2041 atcatcgata cgatctatgc gaaacgtgtg aaccatgtgc cggaactagc acgaatgggc
     2101 gctgatatta ccgtggaagg aaatatgatc atcgtgaatg gtccaaataa acttcacggc
     2161 acagaagtcg tggcttctga tcttcgggca ggtgcttgtt tagtgattgc tggactattg
     2221 gcagaaggga caacgacgat ttacaatgta gattatatct tacgcggtta tgatcatatt
     2281 atcgagaaac taacagcctt aggggcatca attgaaatga tagaggaagt gggagctgaa
     2341 tgagcgatta tttgacaatg gcagaactag agaacaaaac gttaaaagaa gtttatagtt
     2401 atgccaaaga atttaaaatt ccttattaca gtaagatgaa taaaaaggaa ttatctctag
     2461 ctgtgattcg tgcacaagca gagaaacaag gattttatta tatggaagga atattggata
     2521 tcgttggtca agatggttac ggttttttac ggccaatcaa ttatggacca agtgcagaag
     2581 atatctatat ttctgcctca caaatcagac gattcagttt gagaaatggg gacaaagtgg
     2641 caggaaaagc ccgtccacct aaagaatcgg aacgttatta tggtttgatg cacgtggaaa
     2701 gcgttaatgg aaaagatccg gaagaagcaa aagaacgtcc gcattttcca gccttaacac
     2761 ctctttatcc gcaaagacag cttcaactgg aaacaacacc gcaaagacta tctacccgaa
     2821 tgatcgatat cttttctcca atcggttttg gtcagcgtgg attgatcgtc gcaccgccaa
     2881 aagcagggaa aacttctgta ttaaaagaaa tcgcaaatgg tatcaccgaa aactatccgg
     2941 aagtagaatt aatcgttcta ctgattgatg aacgaccgga agaagtaacg gatctagaac
     3001 gaagcgtgaa aggcgacgtg gtatcttcta catttgatct gcagccgcaa aaccatactc
     3061 gagtttctga attagtattg gaacgcgcaa tgcgtttagt cgaagataaa cgagatgtcg
     3121 ttatcttgat ggatagtatt actagacttg ctcgggccta taacttagtc gttccaccaa
     3181 gcggacgtac attgagtggc gggattgacc cagccgcatt gtataaacct aagaaatttt
     3241 tcggggcagc aagaaacatc gaagaaggtg gcagtttgac gattttagcg accgctttag
     3301 tcgatacagg aagccgtatg gatgacgtga tctatgaaga attcaaagga actggaaatc
     3361 tagaattaca actttcacgt gaattatctg aacgtcggat cttcccagcc atcgatatca
     3421 aaaaatcagg tacccgaaaa gaagaattat tgatgccaag tgaagaatta gaagaaattt
     3481 ggaaacttcg aaaaaatatg actggagatt cattagaata tacggaacaa ctgatccgct
     3541 ttttgcgtaa aacaaaaaac aatcagcaat ttttcaaaga atttcaagat gtctcctttg
     3601 gtaaaaaaaa tccgaccaga aatctgaaaa gataattgca accttgactg aaacatgtta
     3661 agatgttaat gttgtaaatc gacaaatcaa ctctggttca gcaaatggag cagggcaaag
     3721 gagcgaaaaa atcatgaaag aaaacatcca tccagattac cacccagtag tattcatgga
     3781 ttcaacaact ggtttcaaat tcttgtcagg ttcaacaaaa acttcacaag aaacagttga
     3841 atgggaagac ggtaatacat acccagtcat ccgtgtcgaa gtaacttctg actcacatcc
     3901 attctacact ggacgtcaaa aattcacaca agcagacgga cgtgtggacc gtttcaacaa
     3961 aaaatacggt ctcaaagacg caaacgctgc ggaataataa aaacgctgat aactcaacgt
     4021 ttagcagact atctgatagg gatagtctgt ttttttatgt tttttgccaa tttaaaactt
     4081 cgtttgatgc tgaaaaagtc gtaggatcat aatgcacaaa acaatgaaaa actagtatat
     4141 agttttaatt atccacttga taaaaacacc ttatattaac gaaatgaaga ggggttattt
     4201 cgaagtaaaa aaccatttac ctaaaattag aagctaagct ttcctttcaa ggaaggcttt
     4261 ttgttttgtt atagacttgc gattgtttgt gagctagatc gttcatttta atgacttata
     4321 cacgcggatg atgggtcttt ttcctctgtt cattcataat attgaacggt acagctagcg
     4381 atgctatact gacctcagtt ccaagaaaac gttaacattt tttgtggggg agaggttttt
     4441 tatgaggttt gatcaattga tcgcagaaga actgattgtt ttcgatccat cgatagattc
     4501 aaaaggatca ttatttgagc gaatagggga tgtgttgaaa aaaacaaata aggtaagaaa
     4561 tgttcaaaaa gtcgttaagg cactattcaa acgagaggag gagatctcca caggaataga
     4621 agatggattt gggattcccc atgcaaaaag caaatatgtg acaaaaccag cactgtgttt
     4681 ttttcataca gccaaattaa cggactatag cgggatagat gaaatgccaa ttgaatatat
     4741 ttttgccatc gtagttcctg aaaaatcagc agatatgcat ctagagattc tcagcacact
     4801 ctccagacga ttgatgaatg cagattttag aaagggaata aaagaagcca ctacgccagc
     4861 agatatcatc agacagatta gtcaagaatg aaaatagtag agtctttgtc cgagtatcga
     4921 tagtgaagaa aaaggaggaa aataatcatg aaaatcgttg gtgtaactgc ttgcccgact
     4981 ggaattgctc atacttatat gtcggcagaa aaattagaag taacagcaag aaatctaaac
     5041 catcaagcaa aattcgaaac acaaggtgta aaaacagaaa atcagttatc tgaacaagaa
     5101 atcaaagagg cagatgcaat cattcttgca gtagataaag aaatcgaatt ggaccgattt
     5161 gccgggaaaa aagtaaagcg tgtctctaca agccgagcaa tcaaagaacc gcaagtagta
     5221 atagacgaag cattgagaga tattggcgta ttcgttgtgt ctaatgaaaa agaaccagct
     5281 gcaaatgaga aaaagccgac aatctataat catttcatga atggtgtgaa ttacatgctt
     5341 ccatttgtga tcgcaggcgg aatcatcatc gcactgagtt ttgcctttgg gattacagcg
     5401 gctgatccca actcagcaga ctataatgtg ttagcagctg cattttcaag gatcggagga
     5461 gatacagcgt ttgccatgat ggttcctgcg ttaggcgcgg ggattgctac atcgattgct
     5521 ggaaaagcag gttttgcccc aggaattgtt gccggattac tggcctctac aggtggttca
     5581 ggttttcttg gcggaatgat tggtggcgtg ctagcagggt atatctgtga ttttctggca
     5641 aataaaatta gcgtaaaaaa agaattttct gctatttatc agctgattgt cgtccccttt
     5701 ttatcgatct taattattgg gcttttgatg gtatttgtga ttgaacaacc gatcacatgg
     5761 gtgttggatg ctttgacaaa ttggctgaat agcttgggaa atacatccgg gctattgttc
     5821 ggattactta tcgggatcat gatggcagct gatatgggtg ggccgatcaa taaatcgact
     5881 tcgactttct cgatcggttt gatgtcagct ggagtaaatg caccgattgc tgcttgtatg
     5941 gctgcaggaa tgacacctcc cctaggtatt gctttagcca ctttattatt caaacataaa
     6001 tttaccaaag aagaacggac ttcaggtcaa tcatgctgga ttttaggcgc ttcgtatatt
     6061 acagaaggtg ctataccgtt tgcggtatct gacccgatcc gcgtgatacc tagtttgatg
     6121 acaggttcag cagtcgctgc agggatttcc atggcagtag gagttacttc aatggcacct
     6181 catggaggga tttgggtgat gctaattcca aatgttatga acaatttacc tatgtatgtc
     6241 ttggcaattg catcaggtac agtcactaca gcctttatgc ttggactatt gaaaaaaaca
     6301 gtgaaagaga aaaaacaaga caagtggacg aaaaatgaaa atctaacaaa tgaggtggaa
     6361 gcgtaatgtt atatacaatg aaagagttat tgaaagtggc taaagagaat caatttgcag
     6421 taccagcctt caatatttgc agctatgata tgatgaaagc aatcatcgaa gaagcagaga
     6481 gattagaagc accagtaatc ttagaaattc atcctgatga aatcgcctat ttaagtgatg
     6541 agtttgttgc ggcagtaaga gcctatgctt atacaagtaa actgccgatc gttatccact
     6601 tggatcatgg cggtacgata gaagatgtct tacgggcgat tcgaaacggc tattcttccg
     6661 tgatgatcga tgcgtctttg aaatcattcg acgacaatgt acagattacg aaaaaagtgg
     6721 ttgcgttagc tcatgcggta aatgtctcag tggaggctga gcttggaacg attgggaata
     6781 atggttctgc tgaaggaggt gctgaaacaa tcatttatac agatcctgat caagcgaaga
     6841 tttttgtgaa agaaacagga atcgatacac ttgctgtagc aattggaaca gcgcatggct
     6901 tgtatccgaa aaacagaact ccaaaattaa atatcgactt actgaaagaa ttgaaccaac
     6961 aaatcgatct tccatttgtt ttacatggcg gttcaggtaa tccggacgca gaagtcagcg
     7021 aagcagtaaa atacggagtt gccaaagtga atttgagctc agatcttaaa agcgtctttt
     7081 tcaatgcttt gagagaaata ttgagtgaaa atccatcgat gtacgagccg aatgcaattt
     7141 atccagcagc taataaaaag gtacaagaag tggtcaaaca taaaatggaa attttgaaca
     7201 cagtaggcaa ggctagttta tactaaaaat aaaaatgtgt ggtcaagagg gtatgacaat
     7261 agtcttgtca taccctctta accacacacg atgttcagaa gtagtttttt cgaaaaattt
     7321 cttcttattt ctcgaagcca atcacttctg tcacaacctc ttttttcctg ttactatata
     7381 aagtaaatgg ggtgatttta tgttgaatga agtggagaat agacagcagg agattgtgaa
     7441 ttatctaaaa aacaaccaat ttgcaagagt caccgatttg atcgagttag taaattatag
     7501 cgaagccacg attaaacgtg accttgtttt tctggaaaaa agtggattga tccgtagagt
     7561 tcgcggtggc gccatgctta ttgacaatcg aaaaattgac gtgccttacc tgatgaagat
     7621 caccaaatta gaagaagaaa cagaaaaacg taagattgca gatacagctt cttctttgat
     7681 tcgagatgat atggtcttat tcttggattc cagtacaacc tctttacact tagtcagaaa
     7741 cctagcaaga tttgatggtt tgcagatcat tacgaatggc atcgtcacag ctgctatgtt
     7801 gagtgaatat acaacggcaa aagtatctgt attgggaggg gcaattgttc cgaaacgagc
     7861 aacaatcaac ggttcaaaag cctataacga tgcattgtca tattatgcgg atttagcgat
     7921 aatgagttgt cgcgggctag atttcagcca aggagtaaca gaaactcatg aaggagaggc
     7981 aatggtcaaa aaagctttta gaaaacaatc agaccggctt gccgttttag ttacacagca
     8041 aaaaattgca cataaattca tgcatcaagc cattgctttt catgaaatcg attatctggt
     8101 gacaagtaaa gaactgacaa aagaacagta tgcgaaagta aaggaacacc atatcgaatg
     8161 tctgtacgga taaattatta cgtgaatgtt ctaagtggct tcctcatttt tagataggac
     8221 cttatctttg ctcgcttctc tcagccgatg gtataataat gaaaaggtta tcttgttaga
     8281 taatcttact tttttatcca ggagaaagga gtgcagaaaa tgaaacatgt gaaggcgttg
     8341 gtagtgaagg caatcatgat ctgggcgatt ctgtgggtcg tgctgacagg tttatacggt
     8401 gttagcttta tggattcaac gattgttggg gtaattattg tagtcatgat ctatgtgcta
     8461 ggggatctgt tgatcttacg taaagtaggg aacatcgcag cgacgatcgc agatgctggt
     8521 tcagctgcag ttatcttgtg gctctatcta tattttatga atgatacagt cgatatttgg
     8581 atgaaagtat tgattcctgc tctattgatc ggtgtggcag aatggttctt ccacaagtgg
     8641 ttgctaagtc aaggcatcgt accagatgaa cggaaaatgg aataaatcaa cagttaaact
     8701 attcttatcg ataaatgaaa gctttaaaaa taaatagatg tttaattaag aataatcata
     8761 aactattttt actcaaaaaa ccgctcagat tattttcaga tctgaacggt tttttttatc
     8821 tattcatcaa gatcaaattt catttttcaa tgattcaatc aattcgccct ctgcttcatg
     8881 actatgtttt cggccattaa caagatagag tgctgtacat atcaatggaa tgactccggc
     8941 aatcacatac aatcctctaa atgaagtaag agattttagc atccccatca aataaggacc
     9001 aacgcctaag cctaggtcta aaccaataaa gtaggtagat aaagcaatac tgattcgatg
     9061 ttcgcggacg atttttaaac aaacagcctg tccatttgac ataaacgtac cataacctag
     9121 gccgactaat gctccagaga taagcaatgt cacactattc gtcgttacac tcagtaaaaa
     9181 taatcctgct gttaagaata aatagctagg atacatcacg tattgttctc ctttgacatc
     9241 gaaaatcctt ccagtcaatg gacgtgtaaa tgtgatgacc aaagcatata cgacgaagaa
     9301 aaatgaccca gcagcaacta agtggattac ttttgtataa gaagataaaa atgataaaac
     9361 acttgaataa gaaagaccca tcaaaaaggc aattcctgta atgaaaagtg ctttcttttc
     9421 aataaaacta ttgatattcc atgagcgaag agatattttt tgttcttcag ataatggaat
     9481 attttttaca ggaaagacaa ggcaggcaat cgtaacaaag aagaccagta caattgaaaa
     9541 ccagataatg aaacgaaaat cagtcatatt cagcaaaatc attcctaaaa atggcccaac
     9601 agcggcagct aaactagtac ttaatccata ataattgatg ccttcccctt ttttagatgc
     9661 agggatatat gcagtaacga tagcatttgt agctgtagaa actgtaccgt agccgaatcc
     9721 attcaagaat cgaactaaat acaatgcccc gatagttggc gtataaagat atgctaatgt
     9781 tgaaaggaga tagaagatcg cgccgcctct taaggtaaac ttccgaccaa atagttctaa
     9841 ctgtttcccc atgaataagc gtgccagcaa tgttccgatg atatagatcc cagaagcaaa
     9901 acctgcttgg ctcaagctgg cgtgtaaggt gtcttgcgca atgactgcca tgatgaccat
     9961 caacagataa taaatcaggt agacaataaa attgatcaat gtgattccaa taaaatattt
    10021 attaaataat ttcttttcca tgtatgttat acattcccct taagctgaat atgcttttaa
    10081 tttttgtatt tgaacttcta catcgtgata attggatgga tagcgatcaa caagttggcg
    10141 aataaataaa tctcttgtag acataaacag atttaaaaat tcttcgcatt cgtttgttgg
    10201 attctcatcc actgctttga tcaattcgat cgagttttga tacgcatcat ttaaaaagac
    10261 taaagaagct ttcatatcaa atcttgtttg atatgctgtt tctaatcgat taatgattgt
    10321 gttcatccag taattcatgt tttttccctc tattcttaaa aaattttact tctgcagtat
    10381 aacgaataaa aaaaaggaaa aaaatcgcat aagtgatgga aaaaggtact taacaaatgt
    10441 taatatcatt agtaaaatac ctttggactt taaaaagttt taaagtactg tatgatcttt
    10501 ttagatgaaa gtttggatgg gggagcatca ttggatagta gagtcttaaa ggaatatttg
    10561 gataaaaatg attttccgat tatacgcaaa ggatatcata aatatttgac attcgaaggg
    10621 atggaagatt cccatacgta tatcttgaaa gagggaacgg taaaagcaag tgttatttca
    10681 aatgatggga gagaatttaa ccttcgctat atcaaagatc ttgaaatcgt ctctctttta
    10741 aaagacgaat attcacaatt catcgattcg ccgtataata ttcgagttga atcagaatat
    10801 gcagagtttt atcgaatcaa tcgtgtgcgc ttttggcaaa atatcaacca agacccaaag
    10861 ctacaacact atgtaaaaga gtattaccgc tatcgtctgc tttattcgat gaaaaaaatg
    10921 cagcaaatgc tattaaacgg aaagctggga gctgtttgta cgcaacttta cgaattatat
    10981 gatttattcg gtgtgcggac agaagaaggg atgctgatcg atttttcgat cacgaatgaa
    11041 gaaatcgcca aattttgtgg catctcaact gctagcagtg taaacagaat tttaaaacaa
    11101 ctgaaaaaag atggtattat caccgttaaa gaccatcata tcgttatttt gaatatggac
    11161 ttgttagaag acaatattat cttttgatga acaaagaggt tgtgaaatca gcgttttgac
    11221 ctgagtatta gagaaacaaa cggaaaaata gttcctcata ttttgccgtt ttttgagcta
    11281 ataccgaggg tcagctgatt gaacaccgtt taccaagagg ttgtgagcct gccgtttaac
    11341 tctgagtcac aaggaacaat ttcaaaaaac agcttttcat gtttgatgaa tattgtgact
    11401 tgtgaccgag gagttggtag gagaacaccg tttaccaaga ggttgtgaaa aagctgttct
    11461 gcatcaagta ataagaagag ccaagtaaaa acagtctctt atgtttttaa cttggctgga
    11521 cttatcaccg tagatgtaag cttttgaacg ccgtttattt gcaacaaaag gggctgtgac
    11581 acgatttttg tcacagccct ttttttgcga acattcattt agcaattttt agaaacttct
    11641 gccgccaccg ccaaaagtcc cgccaccgct agaatgggta gtacttccgc caccgccgct
    11701 tccaggaggt gggcttttgg ggattcttct tgtcgtgaca aaagaatttg tcagacgatc
    11761 ggttttttca gttaatttga tactggattt ttctcggaat ggatatttgt agatgcctga
    11821 tttcaattga taacgcgaaa cagtcacaaa ataaaagaca agactaagga taagagccaa
    11881 aacaacagca atgatgattt ctactgtcgt caaaacctta tagcgtgtaa ttttgccagt
    11941 ttcttcgtct acacgataat gtcctcctgg aactccgtct tccacatatg ttgctgattt
    12001 agacaggtaa gcttttgcag cattgtaata ggcagcattt gtcatctgat cataaacatc
    12061 atctaagatc gaatcgattc tgctatcagt caaataatcg atcatattac cagaagtcga
    12121 aatgtagatt tcacgctgtc ccatatctaa tagcaatact gagccatttt gatcatttcc
    12181 gactgcgtct cttaagtaat tatcagcaaa gtctcttggt tcttctgaat tagatgtagt
    12241 cgttacgatc aagactcgtc ctttgatttt ttcattcagc ttttctgcct gttgttccaa
    12301 agattggatt tgatctgttg tgaataaccc agcctcatca tttaccgtag ctgtatctgc
    12361 atgagtagcc accggccagc caacacttac tagcaaaaga aagaataaaa aaaatttttt
    12421 catcatatca agtaacctcc gatcagaaag aggatcgcta gcccagcgaa aatcccaaga
    12481 gcggtcagtc ccaatttttt ctggctgatc ggcaggacac cgctgacctt tcctgtttgt
    12541 ccattcattg cgtagtagta gactttttta cttgagtcat tgcttcgata agtaacaagc
    12601 cataccggca gtaatacgta attatttact tcgtttgtga ttgaagcaga agttcgcaca
    12661 ccggtcaatg ttgtatatcc atttgcggta tcttttaaca gcttctctga atagtctttt
    12721 aattcagatt gaatctgtga tttgattgcc tcatattcga tatctctttt ttctgcttgg
    12781 aacccggcta aatactggct tttaaaatca attgctttat ctaacgggaa aggttgcacg
    12841 ccttcgacca tcttttgttg cgtatttttt gataaggcat tttttactag ttctttaaaa
    12901 gaaagcttgc ctgctcttga aacagcaaat tgcttcgttt ctgtgtattc gatgtcacct
    12961 atccgccata tgcggataac tgtaccattc gcctgcatgg caccatctac ttcagcatct
    13021 gtagcccaat aagggaaata cacacctgtc aacttatcga tttgatcctt attgaaaaaa
    13081 gctttgggaa caaaccattt tttcttggtc caagcgagaa atctgttgat tgcttcttct
    13141 ttctcgacag caaaaggaag tactttattt ggcaagaatt ctccgcttaa acgtcccgat
    13201 agtacgacgg gattatggca gtagtaacaa taagtcgcag cagttgtcgc atcagtcacg
    13261 atctctgcac cacagttggg acaaagaaaa agatctatcg ctgcggtatt atctgcagat
    13321 tcgtccgctg tatctgaaaa accgcctgcc tcgtcaaccg ctttgcgctc ggcttcattc
    13381 atatcggcaa gctgttcttg ggctgtaaaa gtaaaatctt cggtaggtga aggttcttct
    13441 ttgctttctt cgccagccat acgcgcttct ttttgctttt gctcatattg gctgacttct
    13501 tcttctgtat agatattcag gcagtattcg caatgaaatt tttggtcttt gggatcaaaa
    13561 gtcaacggac cgccgcaatt aggacattta tgtgtaagaa catccatcag ctgcacacct
    13621 ttctaaataa attaattaac ggggattcct ttaaacggtt taccacagtt tggacagaat
    13681 ttagggatgc cgttggaaag atccacgatc tcattgcatt cactgcatcg catttgtagc
    13741 ttaggctgaa cgttttctac aggtttggca gctccgcagt tggaacagaa ttttccggaa
    13801 ttttctgtcc cgcaaactgg acatgtccaa gtatccgcat ttccttgtgc agtttgttga
    13861 ttttgttttt ctgcttgttg tttgatttgt tcttggttat tttgagctgc ttgagaaaga
    13921 taagaaccat ttgcattcat tcccatacct acacccataa agccggtcat tgctcctgct
    13981 tcattttttc cggctgcttc cataccacgt gcaatggaac cttgcacgta tccttcacgt
    14041 acgctaggat cacctagcat cgccccttcg ttacgcatat tgatcagttt cacagaatca
    14101 tcggtgtaag aaatgctggc tacagccaca gaaacaatct ccatcccacg taattcacgc
    14161 caagaatcat ccaatgcagt atccatgtat ttgcttaatt ccaaactctt agatggtaca
    14221 taagaaatcc gttgaccatc tgcagacatc tgattgattg ctgattgtag cgctgttagg
    14281 aattcagcta aatactgctc attgatatca ttgatttcca cttgtgtttt gtttttaggg
    14341 attgcgtttg tataaaataa aatcgggtca acgacatgaa tagaataagt gccatgtgcc
    14401 cgtaagaata attctgcgtt atagaaattg tcaaagtagt tcaacggcgc gcttgtaccg
    14461 aatttgattc cctttatttc ttggagattg atataaaaaa cttgttgttt ctgcggtgtg
    14521 acgccgccaa atttgaatcg gtcaaaagtt tcagcaattg cttctttcag tgttccattg
    14581 aacatggaag gagctgcgtc atttttaatg gtgtaataac cttcctcagc ggtataatcg
    14641 atgatttttc caccatcgac caaaagcatc atcatgtttg gatagacatg gacgactgtt
    14701 ccgtctgtta aaacatcttc tgttccttta cgattggagc cacgtttgtc atctttgcgg
    14761 actttgacac cttttgtcat gaccgttgta tcactcatat tgtctggttc gatgacttct
    14821 agccattgat ctgccagacc gccgccaacc atacttgttg ctgctttgat aagacccata
    14881 aaaaattcct cctctgattc agctagtgcc aaacaattat tgtttattta ttataccata
    14941 ggaagatgtg aaacttctcg gactagagac ggaattgaaa tggaaaattg cgtattgttt
    15001 aaatacctga aaaagccgag aaggatagta gtttttttac ctaatatcac gtataataag
    15061 agatagtgaa ttttttaaaa aagggggatc cttatggcat atcaagcact ttatcgagtc
    15121 tggcgttcgc agcgtttcga agacatcgtt ggacaaaaag ctgtgacaca gacactaaaa
    15181 aatgctattg tttcccacaa aacctcacat gcctacttgt ttacaggacc cagaggaacc
    15241 ggaaagacga gtgcagcaaa gattttcgca aaagcaatca actgtccgaa cagtaaagac
    15301 ggtgagccat gcaacgaatg cgaaatgtgt cgatcaatta ctgctggtac gcaagaagat
    15361 gtgatcgaaa tcgatgctgc cagcaataac ggagtcgaag aaatccgatt tatccgtgat
    15421 cgggcaaatt atgcgcctac aaaggcagcg tacaaagtgt acattattga tgaggtacat
    15481 atgctttcaa caggagcttt caatgccttg ttgaaaacat tggaagagcc aaaagaaagt
    15541 gtcatattca tcttagcaac gaccgaacct cacaaaatac cagctacgat catttcacgg
    15601 actcagcgat ttgattttaa acggatcaat acggcagata ttgtcgaaca tcttgaattt
    15661 atcttgaaag aatcgactat cagctatgaa ccagctgcat tgaatgtgat tgcccgttct
    15721 gcagaaggcg ggatgcggga tgcattgagt attcttgatc aggcaatctc gtttagtgaa
    15781 ggaacaatta ctttagatga tgccatgcaa gtgacaggta gcttaactga cgaaatgatg
    15841 gacagttact tgagtgcttg cgtgaaaaaa gaagtaccag aagcattgga aacattgaac
    15901 caaattctcg cagccggcaa agaatcacgt cgctttctag aggatatgtt acaatactgc
    15961 cgtgacttgc tgatgtatca gcaagcacct aacctagttg aggaacaatc gagtacgatc
    16021 agaagtacat ttacagaatt ggcacaaact gtttcagcgg atcagctgtt ttcaatgata
    16081 aagattttga gcgatactca aaatgaaatt cgttttacca atagtgccgc tatttatttg
    16141 gaagtcgcta cagtcaaact tgcacagcta ccgtctgctt ctgttccttc agcagatgta
    16201 gcatttgcag atgatcagat agtaaaacag ctacaacaag agattaagga actaagaaac
    16261 gaaatcaagg aactacgcag taatacagga attgagaata caaaagtaca gccggtacag
    16321 caaacaaatc ataaaaaaca gacaagtacc tatcgtgtac cgacggaacg agtatataaa
    16381 gttctgcggg aagcgacaag acagcatctg aaccaagtaa aagaagtatg ggaagacttg
    16441 ctgatgagtc tatctgtcac tcagcgagca atgcttaaag caagcgaacc agttgcagct
    16501 ggacctacag gactagtgat tgcgtttgat tatgagattg tttgtcaaag agcagcaaat
    16561 gacgaagaat tgcagctttc ggtacacaac aacttaagcc gaatgatcaa agattatgcg
    16621 cctgatcctg tttatattac aagagaaagc tggccagaac ttcgaagaaa ttatttgacg
    16681 caggcacaag aagagggaac agaaaactct tttgcttctg aagactcaga aggagaaatc
    16741 gagttgcttc ctcaagaaca agaagtacca gaagaaacac ttgtagtaaa agcagaggaa
    16801 ctgtttggct ccgaagctgt tacagtggta gatgattaat aaaaaggagt agataattat
    16861 gatgcgtgga atgggcaata tgcaaggcat gatgaaacaa gtacaaaaaa tgcaaaaaga
    16921 aatggcagaa gcacaagagc tgctaaataa aacagagttt acaggtaaag ccaccaatga
    16981 attggtcaaa gcggttttta caggcgatcg tcgcatgaaa gatatccaaa tcaatgaaga
    17041 agtcgttgat cctgatgata tcgagatgct tcaggatctt gtcgtaatgg cagtcaatga
    17101 tgcgatcgca caaattgatg tggaaactga gaaaaaatta gggaaatacg caaaaggttt
    17161 gccaggattt tagttttaaa ctgaaggagt aatgaatatg caatatccag aaccaattgc
    17221 gaaactgatc gacagctaca tgaaacttcc aggaatcggt cagaaaacag cgacaagact
    17281 cgccttttat acgatcgata tgaaagatga agtagtaaat gaatttgcta aatctttgtt
    17341 aagtgtaaaa cgcgatttgc atttttgtag tatctgcgga aacatcacag aagaagatcc
    17401 ctgcgaaata tgtagaaatc cttcacgtga tcgaagcgtt atcctagtgg tcgaagagcc
    17461 gaaagatgta atggcaatgg aaaaaatgcg agaatatcac ggactttatc atgttctcca
    17521 tggtgtgtta tcaccgatgg aagggacagg tccagaagac atcaatattt cttctctgtt
    17581 gcagcggctg catgatgcag atgtaaaaga agtgattatc gcaacaaatg caacaacaga
    17641 gggagaagca acagctatgt atctctcacg tctgatcaaa cccgcaggta tcaaagtgac
    17701 tcgtctagca catggtctgt ctgtaggctc tgatatcgag tatgcagatg aagttacttt
    17761 attgaaagct gtcgaaggaa gacgtgagtt ataaatttct aattaagtgt tttttcagat
    17821 acaatataaa agcagcacaa gagatggggg caacaatgtg aacggactat ttattacaat
    17881 cgaggggcct gatggtgcag gaaaaaccag catcttgaat gaactttacc ctcttttgaa
    17941 agaagtagca aaaacagaaa tcattcaaac aagagaacca ggaggcattc cgattgctga
    18001 agagatccga gcagtgatcc ttgatccaaa aaatgatcgg atggatgaac gtacggaagc
    18061 actgctttat gcagcagcaa gaagacaaca tctagtcgaa aaggttctgc ctgctctggc
    18121 aaaagggaaa atcgttttat gcgatagatt tgtcgacagt tctcttgctt atcaaggtgc
    18181 aggccgacgc atcggcgtta ccgagattgc tcggttgaat gagtttgcta cagaaggtac
    18241 tacgccggat tttacccttt atcttgacgt agattcagac accggattaa gaagaatcaa
    18301 gaaaaacaga caaaatcaaa tcgaccgttt agattcggaa ggtttggaat tccaccagcg
    18361 tgtacgtcat gcttacttaa agctggcaga agagaatccg gaaagaatcc acaaagttga
    18421 tgcaagaaaa agctttgaag aagtgctgca aacaagttat catacaatta ttgaacagta
    18481 tccgcaattt tttgaaaact aggaaggtga acgtttatga aaattatttt agcgattatc
    18541 caagacaaag acagcaaccg tttatcaaat gaattgattg atgcaaatat ccgtgccaca
    18601 aaactatcat cgacaggcgg cttccttaag gctggaaaca gtacgtttat cgtgggtatt
    18661 gaagatgacc gtgtagaaga agcattggaa atcatcaaaa aaacgtgtga atcaagaaag
    18721 caatttgtat ctacacctgt tacactggat atttcgatgg atgggggcgt cccttatcca
    18781 gtagaagtag aagtaggcgg tgccacagtc ttcgtcttac ctgttgaagg attccatcaa
    18841 ttttaggagg agctgagcta tgaatcaaga aataaacttg gctcaaatgc aaccgatcgt
    18901 gtatcagcag cttcaacgga gttttgagca tgggcgtatc gctcatgctt atctttttga
    18961 aggggagaaa ggaacaggaa aacatgaagt aggtatatgg cttgcacagc acctgttctg
    19021 tacccaaatg aaagacaatc tgccttgtgg aaaatgcaac aattgtcagc ggatcgaaaa
    19081 gaaagaacat ccagatgttt tggttattga accggaagga caaaccatca aagtcgatca
    19141 gatcagacgt ttgcagacgg aattcagtcg cagcggttac gaatcaagaa aaaaagtttt
    19201 tttgatcaaa gaagctgaaa aaatgaatag cagtgcggca aacagtttgt tgaaattctt
    19261 ggaagagccc cctggagatt ttttagcggt gttagaaaca gatgcagtag gaagaattct
    19321 ccctactatt caatctcgtt gtcaagttct gcattttcaa gaactttcga aagaagcact
    19381 gatccatcga ttgcaagaag agcagattcc attggaaaaa gcaaaacttt tggctttctt
    19441 aacaaacagt ttaggaaaag cagttgaaat ctctcaagat gaatggttta atgatgctaa
    19501 ggacttgatt tatcaatggt tcgtttactt gcaaaagcaa gatactcaag cctttatcta
    19561 tgtccaaaaa aaattggtaa aatcatttaa agaaaaaaca caacagttta caggattatc
    19621 tgtcttgatg ttttattatc aggaagcaca gcgtcaagca gtagcaaacg gcgaatatgg
    19681 caaagtgaaa cagataaacc agataattga acggatattg atcgcagagc agaaactacg
    19741 cagcaatgtc agttttcagg ctgttgcgga acagttcgtt ttgcatacga ttttttagct
    19801 tgccgcaaag gatgatgatc aaggaggatg gaaatggtag aagtagtagg tgtccgatat
    19861 agagatgcag gtcatatcta ttattttgca ccaggagact ctgaatatgt ttatagtgac
    19921 aaggtattag tcgaatctca gcaatcctta catattgcta ctgttgtcct tcctaaaaaa
    19981 gagattgatc ccaatgacct tcctgaagct atccgtccga tcttgcataa agcgtcacaa
    20041 gctgaattag aaaaagacaa aaaaaacaaa aaagatgctt atgatgcatt gaaaatcgca
    20101 aaagaaaaag tgcagttgcg tgagttggaa atgaaaatgg tcaaagccga atacacattt
    20161 gatcgaaata aattgatttt ttctttcaca gcggacgggc gaattgactt tagagaattg
    20221 gtaaaagatc tggccgctgt atttcgtact cgtattgagc ttcgacaaat tggtgtccgc
    20281 gatgaagcaa agataatagg cggaattggt ccatgtgggc gtccattatg ctgttcttct
    20341 tttttaggcg atttcgtgcc tgtatcaatc aaaatggcaa aagatcaaag tttatctttg
    20401 aatccaacaa aaatttccgg tttatgcgga cgcttgatgt gctgtctgca atatgagagc
    20461 gaagaatacg agacagcgaa aaaagaactc cctgactttg gaaaagtaat tgctacacct
    20521 gatggtaaag ggaaagtcgt agggttgaat ctgcttagcc gtatcgttag tattcggctg
    20581 actggacggg aaaatcctgt cgaatatgaa tgggatgaat tgaaagaatt tatccaagta
    20641 ggtgagatga atggataaga gatcattata cgacggattg aatcaattag aatcagagtt
    20701 gagaaataca ttaacagaac ttgttgaaat gaagcatgct ttgcatgaga tcgtagaaaa
    20761 aaatacaact ttggagatgg aaaatcaacg tctgcgggaa cacctgcggg aattgaacca
    20821 agttgcacaa gctccagctg aaaatgtgaa gcaggaactt tctaagtctc gtatgaatct
    20881 tgaaaaaatt tatgaagaag gattccatgt ctgctacgat ttatatggat caagaaggga
    20941 caatgacgag ccttgtgcgt tttgtctaga agtgatctat cgcgaaagcg gtcgatgacc
    21001 aataacaaga ggagactgga gcggagaaat actgctccag tctttttatt aaggagattg
    21061 tgacagatgt ataatcaaaa aagtttcaag ggaaatccgg tatatgggga tttatatctt
    21121 gtaccgacgc caattggaaa tttagaagat atgagctaca gaagtgttcg tatgctgcaa
    21181 gaatccgatc ttatcgcaag tgaggatacg cgtaatacac aaaagttatt gaatcatttt
    21241 gaaatccata caccccaaaa aagtttacat gaacataatt ataaagaacg tgtccctcaa
    21301 ttgattgctt tgttaaaaga aggaaaaacg attgctcaag tcagtgacgc aggaatgcct
    21361 tccatcagcg atcctggaca tgagctagtt ttagcctgca taaaagaagg gatttctgtg
    21421 atctctatcc caggacctac agctgggatg acggctttga ttgcttcagg gctagtgcct
    21481 caaccatttt tattttatgg cttccttcca cgtaagaaaa aagaacagaa agaaacattg
    21541 aaaaagctaa aagaacagac agttacgctg attttttatg agtcacctta tcggatcact
    21601 gctacattga cgaatttctt agaaattttt ggaaatcgcc aagtcgtttt gtgtcgggaa
    21661 ttgacaaagg tccatgaaga ataccttaga ggaagtacgg aagagctact cgcttatatc
    21721 gaagaacatc cagtaaaggg tgaatgttgt ctgcttgtag aagggaattt gttcgaaatg
    21781 ccagtgacgg aagttgatga agagttggga acgttaaagg aacaagtaga agcaaagatc
    21841 gcttcaggcg aaaaaccaaa tgctgctatc aaagcagtag cgctgaaaaa tgggctgaaa
    21901 aaacaagaag tctaccgtca gtaccatgaa ttagactgag atagtataag ttgtctataa
    21961 tgaaagacta atgaggaaat cagctactaa cttttcaaat ctcttgaacg cgaacgaatg
    22021 ttcttgtata atgaataaga ggtgaggatg atggagctgc aatttccttt acataatgat
    22081 acatcaagaa aaatcattca tattgatatg gatgcttttt tcgcttcggt tgaagaacga
    22141 gatcatcctg agtttcgcgg acatccatta gtaattgcca gacaccccag tgacactggt
    22201 gggcgtggag tagtgactac agcaaattat gaagcaagaa aattcggaat tcattcagca
    22261 atgagtgcac aaaaagcgta tgagctttgt ccgcaagcca tctttaagcc tggcgaccat
    22321 cataaataca gcgatatttc caaacaagta cgggaagtat tctatcgtta tacagatatt
    22381 gtggaaccag ttagtatcga tgaagcgtat cttgatgtga ccgaaaataa aatcaattgc
    22441 aagtcagcca tcaaaatcgc ccgaatgatc caagcagata tttgggagag tgtccagcta
    22501 acatgttctg cgggagtaag ttacaataag tttttggcca aattagcatc agactatcaa
    22561 aaacctagag gaatcacggt agttgcccct caagacgctg tgccattttt aaaagctttg
    22621 ccaattgaaa aatttcatgg ggtagggaag aaaactgtac ctagaatgca tgagctaggg
    22681 atttatacag gaaaagacct atatgaatgc acagaaatga tgttgattcg aaattttgga
    22741 aaaatgggct attctcttta tcgtaaagtc agaggaatcc atgattctcc agtaaacgtt
    22801 acaagagagc gaaaatctgt gggaaaagag catacttatg ggcagccgtt gcaaacggaa
    22861 gaagcagtgc tgactcagtt gcgccaacta gctgaaaaag tagaagaagc actgagaaga
    22921 gtccaaaaac atgggaaaac agtagttttg aaggtcaggt acacggacta ttctactgtg
    22981 accaaaagag tgacattgcc agaatatatc tataaaaaag aagcactctt ttatcaagca
    23041 agccttatat gggaggaaat cttgggagta gaaaaaggaa tccgattgtt agggattact
    23101 ttgacgaatc tggacccgat gacttatgaa aacatcgtcc ttccattatg ggaaaatcaa
    23161 gaaatataaa aacagctttg agagttgaaa ccggaaatcc gttttctctc ccaaagctgt
    23221 ttttatatca agtcttgctt tttgatttgt tcataggttt ctgttgcatc cgtacatttt
    23281 tcccaaatca caacttgatt ggattctaaa gatttttgga cgtcaatgat ccgctggttg
    23341 ctacttccgc gaaattgtag ttttaggtca cgtttgctta attcaaagcg tccgtcaaca
    23401 aggatatcga tttgcgaaag gagttctaat ttatcttctg aatcttgtag caattcatca
    23461 aaggtatagc ctgtccatga ccagatatct tttgtcgtac cgaactcttt tcggatacgt
    23521 cgtacgactt gcagacaaac atctgtattc aaaaaaggtt cgccaccaag taaagttaat
    23581 ccttgcacgt aatcgttgcg caagtcagcg atgatttgtt cttctaattc aggtgtgaat
    23641 ggtttgccat atcggaaatt ttgtgcagct ttgttgaagc agccttcgca ggcaaataag
    23701 cagccactaa cgtaaagact gttgcggaca ccttcaccat cgacaaaatt aaacggtttg
    23761 tagtcagcaa tatagttttg gctgtagtct tttgctaacc attcttttgg ttttggattt
    23821 ctcataaaaa aacttctttc atttaaggtt caaagatata gctattgaag ataaaatcta
    23881 ctcgaccaca taaataagaa caaactgacc taaatccatt gttcaagaaa gcttgttctt
    23941 attttgatag agggcgttca aagatacaaa gccatcgaaa gagggtggtg aaaaaagact
    24001 tgtcatagcc tcttattctg aataaacggt gttcaaaagc ttgcatctgc agtaataagc
    24061 ccaactaaac gaaaaatatg aggaactatt tttgtttagc tattcttatt acttgatgca
    24121 ggacagcttt ttcacaacct catttcatgt gtttcacacg tgaggaaatt tctttatggc
    24181 ggccatgtac catcggacga gcttgcggat ttcctaaata gccacaagta cgtttgacta
    24241 catcacatgt ttttggatca tgatttccac attctggaca ttcgaaccct cgttctgtcg
    24301 gtttgaaatc accttcgaaa ccacattgat agcaatgatc gattggcgta ttcgtaccaa
    24361 gatacccgat tttgtcatat gcaaaatccc atacagcttc taaagctttc ggattttgtt
    24421 ggagcatagg gtattcacag tagtggatga atccacctga acaatatttt ggataatcct
    24481 tctcaaattc caatttttca aatggcgttg ggtttttacg cacatcatag tggaaactgt
    24541 ttgtataata ttctttatct gtgatatttt cgatgatgcc gaatttttct gtatccaaac
    24601 ggcagaaacg gtcagttaga ctttcacttg gagttgaata gacactgaaa tgataaccat
    24661 attcatcccc ccatgcatct gcattagctt ttaattcttt gacgatatca agtgtgaaag
    24721 cttttgcttc tgggtttgtt tcccattcgc caccgaagaa agctgaagca acttcataca
    24781 atccaatgta gcctaacgat acagtagctc gacggttttt gaataattca ttgacagaat
    24841 cggtacgtga aagacgtttc ccgaaagcac catacatata taaaattggc gcatttgctg
    24901 gaatggcttc tttacaacgt tctacacgat agagaagcgc atctttcatg atttctaagc
    24961 ggtcagacag taactgccag aatttattct gatctccttt tgattcgatc gcgattcttg
    25021 gcaaattcaa tgtaacaaca cctaaattca ttcgtccgac atttacttct tgaccatttt
    25081 cgtctttcca cccttgcaag aatgaacgac agcccatagg tactttgaaa cttccagtta
    25141 attcaacgat tttatcgtaa ttcagaatat caggatacat tcttttcgtt gcacattcca
    25201 atgctagttg tttgatgtcg taattaggat cttcagggtt taagttcaca ccttttttca
    25261 aagtgaagat cagtttaggg aagatagctg tccgcttttc actccctaat ccattgattc
    25321 gaatctgtaa aattgctttt tgaatttctc tttcaaacca gttttctcct agtccaaatc
    25381 ctaatgaagt gaaaggagtt tgtccatttg aagtaaataa ggtattgatt tcatattcca
    25441 aactttgcat agcatcgaag atatcttttt tggtttttgc ttttgcatat tctttttggc
    25501 gttctggcga atcgatccat tcttccgcgt cttttagatg ttttttatag ttcaattctg
    25561 caaaaggagc aagcaattca tctgttcggt cggcagaaca gccaccatat tgacttgacg
    25621 ctacattggc aataatctgt gagatttgtg ctgtggcggt ctggatagat tttggcgatt
    25681 cgacttcggc attgccgatt ttgaacccat tgttcagcat gcctttgaaa tcaatcaagc
    25741 agcaattcgt catcggtgta taaggatgat aatccaaatc atggtaatgg atatcgcctt
    25801 tttgatgcgc atttgctata tgtggtggca acattttcag tccgattgat tttccaacga
    25861 tcccggctgt cagatctcgc tgtgtattga aaacattact gtctttattt gcgttttcgt
    25921 tcacgacagt ttggtccttg ttgattagct tttcaatcga aaagttgata tctgttgctt
    25981 tactgcgagc aaaatctcgt ttggtacgat aattgatgta ctcttctgct aattcatatt
    26041 cgtttttttc tagtaatata tgttccacga tgttttgaat ctcatagatt ttgacatttt
    26101 cagcaaaccg gcttttgatt tcttggacaa ccaagtctgc aatcatttga attttttcat
    26161 aagtaagtgg tgataatgag ccgtgaattt tcttttctgc tttgatcagt gcttcattga
    26221 tcttttgttc atcgaaatca acatggcggc catcgcgttt gatgactttc atctgggatg
    26281 tctctggctg ctgagtaacg acagtttttt cttcataatt tactttcatt atcttcaact
    26341 cctgctaaat tgttcttgtc tatcataaaa caaaatcggg gaaggagcaa ctaaatatcg
    26401 gctatatata gtattcaatg tgctgtataa ttggatttct atactatatg tagtgtatat
    26461 tttattgtga aaaaaggtga aaacttgtag ctggtggatt tgttttttgc atatttattt
    26521 ttgctgaata aaataattga ataaaatttt ttattcagtc caagtaaaag tgacaaaaac
    26581 ataagagcaa attattcgta gatatgctac tatgcaaata aatagaaatg aaaaatgaaa
    26641 gaggaggaga agaattggaa aagatgatcg aagtgaaaca tctaagtaaa agctacggca
    26701 gcaaactttc tcccgtcaaa gccttagata atatctcatt cacagtagaa gaaggcgagt
    26761 ttgttgggat tatgggccca agtggtgcag gaaagacaac gctgatgaat atcttgtcca
    26821 cgatcaattt gcctacgatg ggcagtgtct cgatccaagg aaaagaaatc acaaaaatga
    26881 aaaacagtga gctcagtgac tttcgtcgaa aaaaactagg attcattttt caagagttca
    26941 atttgatcga taccctaaat gcaaaagaca atatcctatt gcccctagca gttgaacgga
    27001 tgactaaaga ggaaatggaa aagagagtaa gacatgtagc tcaattgttg aatatagaag
    27061 aactgctcaa acggtatccc gatgaacttt ctgtcggaca gagacaacga attgcagctg
    27121 ctagagcact tgttgttcag ccacaagtga tttttgcaga tgaaccaact ggctctttgg
    27181 actcgaagtc ggctacagaa ttgttgaact acttgaagac catgaatcaa aaagaaaaag
    27241 caacgatact gttagtcacc catgatccat atacagcaag ctactgcgat agaattttat
    27301 ttattaaaga tggtgtgatt ttttctgaag ttgtcagaag agggacaaga agagagttct
    27361 ttgagaaagt aatagacatg caggcgacga ttggcggagg aggaaaaatg aatgctgtat
    27421 aaactagccg ttaaaagttt ttttcggcag tcacgaggct atcttgttta ttttttcagt
    27481 ttgactttat ccgtaatgat ctattattcc ttcagcgcaa tgacctacga tcaatcttta
    27541 gtcagaagag caagtcaaga tacttcgata gatggtggat tgagttttgg tggatttatc
    27601 attactgttg ttttgctttt tttcgtattc agtgccaacc gatttttctt gaatagaagg
    27661 caaaaggaaa taggcatcta tcagcttttt ggtatgaata aactgcagat atcaggtata
    27721 tatgtgttag aaatcatgat aattggattg ttcgcctgta tttctgggat attactcggt
    27781 attatttttt ccaaactctt ttcaatgatt ttggttcgaa tgatggatat ggatcttact
    27841 agtccatttt ttatctccat tccatcagta gtagatactt tatttgcttt tttcatcatc
    27901 cttttagcag tatctgtata ttcaatatgg aaaatatggc gctatccaat gattcgaagt
    27961 tttggagaaa aagaacaagc agatagttca acaatgcgct tcaggacaag gcaccgatta
    28021 ctcgcagtca tcgggttact acttttaacg agcggatatt ttggagctag tcattttagg
    28081 gaaatcactt catggatgat cagttatcat tatcttgata cattagtttt cttttttcct
    28141 tttttaattt ttttcgtttg tgtggtcggt acttatttgt ttttctgtta tacatttcgg
    28201 ttatttttga tcatattgag taaatggaag atttattact atggtatcaa ttttttgata
    28261 atgggtaaca cgcaagttca tttgctcaaa agttggcgta caaattctct gattacagtc
    28321 attctcggaa tttctttaat gacaattggc ggcacggcta gtatctctac tattttatcc
    28381 taccaggaga aagtttcctc cccgatggac tatcagtttg atgttgagac agagaaaaaa
    28441 atcaagccga ttctagcaga ggaagggcag aagataacta aagaaatcac cttgcattat
    28501 aaagtcattg ggagtatcta tagtcaaaat ctcgaagaac ctatgtttca aactgctaat
    28561 ctgatcacgg aaacacaata tcaagccttc agaaaagtta atccagattt gccgaacatt
    28621 tctttgaaat cacctggtca tactgttttg cttgatcaaa tgcaaactct ttttcggaat
    28681 atttcattat atggatctag tattggactg ccagataatc aagaattgaa gattcaacaa
    28741 atgcttccaa gtgtattagg tgacgaaaca atggtatatc taagtcctgt actgattgta
    28801 gatgataccg tttttgaaaa agcaaaaggt gtggattatc aagtagtcaa tgtagatgta
    28861 tcaggtggaa ataatgaaaa aatagatgaa cgactgagca atgacctgtc gataaagtgg
    28921 ctcaatgctg tttattattc ttatgatatc gtaaatggaa gtctacaagg agaaattagc
    28981 acaaagaaac ttccggatga ccaaatgaag gaagcacaat ttagccaaga aagctctcgt
    29041 ttgaatttct cttccagcta ttcgaaattg cgtgcagtta atcggcgaat cggtatcaat
    29101 atttttgtca cactatttgt cgggatgatc gtgattattg ctactggtag tattttgaca
    29161 gtccggcaat tagcagaagc agaggaagaa aaagaaaatt atcacctatt gactaagctg
    29221 gggattccac aaagaaggat cagacggatg atcttcgagc aaaacgcact taccttcttt
    29281 ccacccatga tattagggat cactcacgca gtatttgcaa tcaatgtctt cactcagtat
    29341 ataaaaactg ccgattactg gttggcttat cttgtgagtg gcttattgat ccttatctat
    29401 cttttgcttt atgtcattac gagtcagtta tattgtcgga ttatcgagga atgatatacg
    29461 gaataagac
//
LOCUS       DAZUMK010000018        29344 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000018 DAZUMK010000000
VERSION     DAZUMK010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.018"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(12..2183)
                     /gene="copB"
                     /locus_tag="ACG808_000866"
     CDS             complement(12..2183)
                     /gene="copB"
                     /locus_tag="ACG808_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFS5046514.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(2203..4389)
                     /locus_tag="ACG808_000867"
     CDS             complement(2203..4389)
                     /locus_tag="ACG808_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS5046515.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(4389..4598)
                     /gene="copZ"
                     /locus_tag="ACG808_000868"
     CDS             complement(4389..4598)
                     /gene="copZ"
                     /locus_tag="ACG808_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFS5046516.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4611..5051)
                     /locus_tag="ACG808_000869"
     CDS             complement(4611..5051)
                     /locus_tag="ACG808_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFS5046517.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5125..5664)
                     /locus_tag="ACG808_000870"
     CDS             complement(5125..5664)
                     /locus_tag="ACG808_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HFS5046518.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            complement(6279..7745)
                     /locus_tag="ACG808_000871"
     CDS             complement(6279..7745)
                     /locus_tag="ACG808_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS5046519.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(8056..10137)
                     /locus_tag="ACG808_000872"
     CDS             complement(8056..10137)
                     /locus_tag="ACG808_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS5046520.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            complement(10279..10401)
                     /locus_tag="ACG808_000873"
     CDS             complement(10279..10401)
                     /locus_tag="ACG808_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046521.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAVAMIEKKKKE"
     gene            complement(10661..11020)
                     /locus_tag="ACG808_000874"
     CDS             complement(10661..11020)
                     /locus_tag="ACG808_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFS5046522.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(11050..11391)
                     /locus_tag="ACG808_000875"
     CDS             complement(11050..11391)
                     /locus_tag="ACG808_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046523.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            complement(11388..12062)
                     /locus_tag="ACG808_000876"
     CDS             complement(11388..12062)
                     /locus_tag="ACG808_000876"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFS5046524.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(13239..14537)
                     /gene="asnS"
                     /locus_tag="ACG808_000877"
     CDS             complement(13239..14537)
                     /gene="asnS"
                     /locus_tag="ACG808_000877"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFS5046525.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVPEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(14577..15767)
                     /locus_tag="ACG808_000878"
     CDS             complement(14577..15767)
                     /locus_tag="ACG808_000878"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFS5046526.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFLKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(15788..16315)
                     /locus_tag="ACG808_000879"
     CDS             complement(15788..16315)
                     /locus_tag="ACG808_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFS5046527.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(16362..19151)
                     /locus_tag="ACG808_000880"
     CDS             complement(16362..19151)
                     /locus_tag="ACG808_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFS5046528.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFIYVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGTGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMTTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(19298..19498)
                     /locus_tag="ACG808_000881"
     CDS             complement(19298..19498)
                     /locus_tag="ACG808_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS5046529.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(19950..20270)
                     /locus_tag="ACG808_000882"
     CDS             complement(19950..20270)
                     /locus_tag="ACG808_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046530.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(20315..20458)
                     /locus_tag="ACG808_000883"
     CDS             complement(20315..20458)
                     /locus_tag="ACG808_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046531.1"
                     /translation="MKFRLISEEATSGTPFKKEVVTWSRPLFDDYDTKNIIYEQNVNN
                     GRS"
     gene            complement(20654..21772)
                     /locus_tag="ACG808_000884"
     CDS             complement(20654..21772)
                     /locus_tag="ACG808_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046532.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            21996..22127
                     /locus_tag="ACG808_000885"
     CDS             21996..22127
                     /locus_tag="ACG808_000885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046533.1"
                     /translation="MDLMHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            22243..23550
                     /locus_tag="ACG808_000886"
     CDS             22243..23550
                     /locus_tag="ACG808_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFS5046534.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            23669..24868
                     /locus_tag="ACG808_000887"
     CDS             23669..24868
                     /locus_tag="ACG808_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFS5046535.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYAWQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(24895..26163)
                     /locus_tag="ACG808_000888"
     CDS             complement(24895..26163)
                     /locus_tag="ACG808_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFS5046536.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     regulatory      complement(26218..26284)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(26326..26973)
                     /locus_tag="ACG808_000889"
     CDS             complement(26326..26973)
                     /locus_tag="ACG808_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFS5046537.1"
                     /translation="MEQTIKPYQYFFIKREVEQLLNAYSSVNDPKTVQTVQALATEKI
                     RDILEHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMIYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(27381..27884)
                     /locus_tag="ACG808_000890"
     CDS             complement(27381..27884)
                     /locus_tag="ACG808_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS5046538.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(28007..28771)
                     /locus_tag="ACG808_000891"
     CDS             complement(28007..28771)
                     /locus_tag="ACG808_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFS5046539.1"
                     /translation="MKEIQSAKNPKIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            28879..29154
                     /locus_tag="ACG808_000892"
     CDS             28879..29154
                     /locus_tag="ACG808_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFS5046540.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
BASE COUNT         8265 a         6304 c         5152 g         9623 t
ORIGIN      
        1 ttttttcatt attatttgat tttcaatgtc aaagcattta gagctacaac taccgtactt
       61 aacgacatca agacagcacc aactgctgga cttaatatga taccaattgg cgcaagaatt
      121 ccggctgcta gcggaattgc aagaatattg taacctgccc cccaccagag gttctggatc
      181 attttctttc gcgtttgctt ggctagatca agaaaatgca aaatatcttt tggatcgctg
      241 tttgtcaata cgacatccgc agaatcgatt gcgatatctg tccctgcacc gattgccatc
      301 ccgattgaag cacgggctaa acttggcgca tcattgatcc catcacccac catgattact
      361 tttttccctt ggtcaaggta tttctggaca acagcttctt tatcatctgg taataaaccg
      421 ccataatatt cttcgatccc taaataatct gctactgctt gggcagcgtt tttattgtca
      481 ccagtaagca tgacaggagt gatttttctt tctttcaatg ttcggataaa ttcttttgct
      541 tctgttttta ctacgtcacc taaagcaacg attgcagcta gatgattttc taaaatcagg
      601 aagctgatgg tatttccttg ttcttggtaa ggtttcaata atgcttgttc tacgtttaat
      661 ccgagacgct caacttcttt ttcattaact attttcactt gttgtttttt gactgttgcc
      721 tctagcccga ctcctgaaag attttttaga ttttcagctt gataaggctt gatttcatgt
      781 tctttcaaat aattcataat gccaacagcc aatggatgat tggcattcgc ttccaaggct
      841 cctagatatt gaaggatttc atttttattg aacgcatcat caagtacttc gacacccgtt
      901 acagtaaatt ttccttctgt tagtgttcct gttttgtcca gcatgatcac atctagatca
      961 ttggcttgct ccagtgcatt gcggtttttc aaaagtaatc cgtttttagc tgctattgat
     1021 gtggagcgag caacaactaa tgggatagct aaacctagag catgaggaca ggcgatgata
     1081 aagactgtta ccatacgttc taatgcatta ggaagatctg tcaagaacaa ccaggcgatg
     1141 aatgccagga tacccacaat taatgcgaca taaaacagcc attttgccac tttgtcagat
     1201 aatgactcta gcttggattt ttctccttgt gcttttcgca ccatttccat aaccttcgct
     1261 agataaccat tttcaccagt tccagttact tcgatttcga tcgttccatc cccattgata
     1321 gaaccgccaa tcactttgtc attggcttgt tttttgattc ctcttgattc gccagtcaca
     1381 gctgattcgt caacaattgt ttcacctttc aagattacac catctgttgg cattttatca
     1441 ccagaccgga cgatcaaacg atcaccttcg tttacttctt tcaatgaaac tgtctcttct
     1501 tttccatctt tcgtcaagcg tttcactgat tctggcagta attctgctag tttttgtaaa
     1561 gcattgctgg cattcgacac agcattcatt tcaatccagt gacctagtag cataataacg
     1621 atcaaagtag ccaattccca gaagaaatcc atgacgtgtg tatgtggatt gagcagattt
     1681 gcgataaacg aatagacact ataaatatat gcaactgtaa tccccatcgc gatcagtgtc
     1741 atcatagcag gacttttttg tttcaattcc atcttagctc cacttaaaaa tggctgaccg
     1801 ccatagataa atagtatagt tgctaagatc aatacgagcc aatcagaacc aggaaacgtt
     1861 acttgaaatg gaaattccat tcccatcatt ggtgaaaaaa gaatgattgg gatagctagg
     1921 ataagcgata gccaaaattt ttgtttgaaa ttccccatgt gcatggaatg atccatgccg
     1981 ccgtgttcgt gatccatgtg catcgaatgg tccatttgtt tgctgtttgc tatatctcca
     2041 tggttatgtc ccatgtgcat ggaatgatcc atctgactat ggtcatgttt catctccatc
     2101 tgtttttcat gagattctgt actttctttg tttactactt cttttttatg ctcatgtttt
     2161 ttctccatat cgttcccatt catttttact ccatcccttc tcttactttt tcatgtgtcg
     2221 atttaagctc agggagttca acaatacact gacggaacta aacgccatcg ctcctcctgc
     2281 aatgattgga ttcaaaaagc ctagtgcggc aaaaggaata ccaatcgtat tatagacaaa
     2341 agcccagaaa agattttgtt tgatttttct caatgtcaac ctagacaact cgattgtttg
     2401 tgcaatggat gccaaactgc tattcatcag cgtcacatcg gctgtttcca tcgcaatgtc
     2461 cgttccactt cccattgcca taccgacatc agctaatgct aaggccggtg catcattgat
     2521 tccatcgcct gccataccga cttttttgcc atctttctga agtttttcta cgtaggctgc
     2581 tttatcctct ggaagtactt cagcaaagat atgctttgga tcaataccaa cttgtttacc
     2641 aatcgtttcg gctgccagtt tgttgtctcc tgtcagcata aaaacatcta atcctttatt
     2701 ctggagctgc ttgattgctt gtttggcttc tagtttgatt tgatcagcca cggcaatcca
     2761 cccgatcact tgcttttcat cagctaaaaa catgactgtt tttccctgct gttctaattc
     2821 taacgcgtgt tcttggtatt catcaaatga taggttcaat tcaattaatc gtttacgtgt
     2881 acccgcaaaa tagcgtactc cattaatcgt accgctgatc ccagcacctg ggtgggctgt
     2941 aaaatcaatc atatcgtatg ttgctgcttc ttgcagtttt ccgtattcca cgattgcttt
     3001 acctagtgga tgttcagaag cttgttctaa agtataaaaa atagatagga cttcttttga
     3061 accgactaga tctgtaactt tgggctttcc ttctgtaatc gttccagttt tatccaaaac
     3121 aatgctgtct aagtgagcag ctgcttctaa tgcttctccg cctttaatta aaattccatt
     3181 tcgtgcacca acacccgttc ctaccataat agctgttggg gtcgctaaac ctaatgcaca
     3241 tggacacgca atgactaata cagagacact atggatcaat gcgagttgcc aatcaccagt
     3301 gatcaaacct gtagctatga gcgtgataaa cgcgatgact aatacgatcg gaacaaaaat
     3361 ccctgagatc cgatctgcga ttttttggat tggggctttg ctcccttgag catcttccac
     3421 catttgaatg atttgcgcta atactgtctg cttgccgact tgtgtgactt ccatgtgtag
     3481 cagaccattc gtattgattg tcccgccaaa aagcgtgtca tccggattct tttctacagg
     3541 aagattttca ccagtaagca tactttcatc tatcgcactg cttcctgaaa taattcgacc
     3601 atctgctgga atctgctcac caggacggat gactagttga tcaccaacga ctacttcttc
     3661 aatcgctaac gtttcttctt tcccgtttct tatcacttgg gcggtttttg tttgaagaga
     3721 catcaattgt ttgattgcat tacctgtttt cgtttttgct gtatgttcca aatatttgcc
     3781 gagcaaaatc agcgtgatga tcatactgct gctttcaaaa taaagatctt gggggtgtcc
     3841 acggaaaaat ccgttgtaaa tgctcaatgc aaaggcagcc gatgttccaa ttgctaccaa
     3901 tacatccata tttggtgctt tcgtcttgat tgcatggtac gcacctttat aaaaacgagc
     3961 accgacataa aattggacag gagcagacag cactaactgg acgattggaa aatgaaaaaa
     4021 gtgaacaatc gccgcatggc ttcctaacat catagcaatc attgacaaca tcaacggcaa
     4081 agtcaaaacc gtactcaata taagatcgaa tagcattttt ttcaaatacg cttgcttttc
     4141 ttctgcaatt ttttgtttgt gggcttcatc gtacaaaatt gcaccataac cgatgttttc
     4201 tacgctttgg attaattttt cagtggtcgt cccttcatat ttgacacttg ctttttcagt
     4261 ggccaagttg actgtggcat tgaccacacc aggttgtccg ttcagctctt tttcgatcct
     4321 agcagaacaa tttgcacaag tcatacctgt aatgacgaac gtatccattt tggtttctgc
     4381 tgttgccatc aaatcacctc tgctttataa cccaatccat tgattgcttg acaaatcttt
     4441 tcggattgaa cttttgtttc atcaaacttg accgtaccgt ttgctttttt caaatttact
     4501 ttcacttttt gcacgccgtc aagtgcgctt actgtttctt ctactcttgc tgcacaatga
     4561 ttgcagctca taccttcgat tgaaaattgt tgtttcattt tttcttcctc ctattgatgt
     4621 tgtttacatt cacattgacc tgggatacag ttacattcga tcgtatctac tggttgcttt
     4681 tgttccagtt gtttcaaaat ctcattgata tcttcttgag tcaaagtggc ttcttctact
     4741 agatccgcaa tcgtctctcc aatccttttt gcacagatat gggaaaaaag attttccgtc
     4801 gcactgcgaa ctgtttccat ctccgagaca gctggatgat aaatgaattt ttttccttct
     4861 tgttctgtcc agatcacttc tttttttacc aaacgtccca gcaaagtttt gatcgttgct
     4921 ggtttccaat ccatcgaatc agctaagatc tgcgtgattt cttgggctgt cgtctgtcca
     4981 agtgtccaaa taacacgcat gatctcccac tctgaatcac tgatctttac tggcgttttg
     5041 actttttcca tttatttcac acctttcgtt tacaaatgta atcattagct tatgaataca
     5101 gattacgctt gtaaacaaaa actgtcaatt atttttgctc tctattttca atttagttaa
     5161 aaattcttgt ttctctcgtc ttgttttaag gatgatcagt ttatttcttg cattagctac
     5221 ttggatcagc tgctgtattt tcggttcatt gttttcttca aaatgccaga taaaagataa
     5281 aaattctaag tattccctgt ttagtttctc tgaaaatgat tcaggcatat ctggccgaga
     5341 ttttttgttt atctttcttt ttagagaacg ccaaactact cggtagatgg cgagataacg
     5401 cggcacttta agccaaatga tcttatctgc ttctttaatc ctttcctcta atgtagaagt
     5461 ataatttcct tcaacaatcc atgacggatt agataccata aacgattgct gttcttgcaa
     5521 aaaccacttt tttgcctgaa tactgtagtc tgttgtatgc cataaaaggt ctagcggcag
     5581 tagcggaaag tcggtgattt ctttgatctt ccttgcgagg gtcgattttc ccgacccact
     5641 ttgtccaatc acgatgatct tcattcttct ccctcacttc tctttggaat ttggttcgat
     5701 cataccactt ataacgccgc aaaaaaacaa gggactgtga caaaagtcgt gtcaaagtcc
     5761 cttgtttcga ataaatggtg ttcaatcagc tgaccttcag cattaactca aaaaacggca
     5821 aaatatgagg agctattttt tcgtttgttc ctctaatact caggtcaaat cgctgatttt
     5881 acaacctctt ccttaaacgg tgttctcctg ccaacacctc ggtcacaagg cacaatagtc
     5941 aacaaacatg agaagttgtt ttttgaaatt gctccttgtg actcggagtt aaacggcagg
     6001 ctcactacct ctttcttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccagca
     6061 aaacgaaaaa tatgaggggc tatttttgtt tggctgttct tattacttga tgcaggacag
     6121 ctttttcaca acctctgatt cacggtgttc cagaagtagc ttctgcgaaa aacaagccga
     6181 aacttcacaa aaatttgaga agcaattttc gtaaattcct tcttatttct tgaagctgac
     6241 cacttctgtc acaacctctt gtaacaagta tttctatttt aaggctgaac cactgtttct
     6301 ggttcagtga tttctttgtt tttgtctcga cgagcaattg ctggcaagat catacctaat
     6361 aagatcaaga caattggtgt gatgatattc gtgaataatt ggaaccacca tgctttagga
     6421 tcagcagtat aatctacttt tggcaccatt cctaggatac atgcaaacgc agtgaaggca
     6481 aagcaccaag caccaaagat aaaaccgatt tttggatttt taacaaattt atattcagaa
     6541 ttgaatttct tataagcttt attcaacatc atatatgcca agaacaccca taggtaacgc
     6601 attggcatga caactgagtt caagttcgtc aaccatttta ctaattcatt catatctttg
     6661 ataccaaaga tcggcaacaa aataatgatt cctactagta tacctgtcag aagataacca
     6721 ttcaccaatg tacctttacg attacgttta cgtagccagt ttggtacaaa ttctggatcc
     6781 gcatctgcta acaagatttt tagtggtgca tcaattgaaa aggctaatgc ggagatttgt
     6841 ccaatcatgt ttgtcaatgc gtaaatgatc atcaatgagt taccgatacc atagtaatta
     6901 cctaaaattt gaaaagcact gtatgcccca tttgccatca agtctttagg aatattatcg
     6961 ctagcaaaca gcatacccat tgcgactgag cctaatacag cacaaatccc taccattgct
     7021 gctaagaaga tcatcccttt aggaaattcc tttgcaggat ttcttgtaga gttgacataa
     7081 ggggatattt tttccgctcc tccaactgca aatactagca tcgaaacggt tgtgaaataa
     7141 ctgaaatcaa atgttggaat atatgttttc acttgattca tatgtggtgt ggcaaattcc
     7201 atcgttggat tgatgaacgg tgccgttaca gctaaaagaa taaataagat cgacatgacg
     7261 aacatcgctg ttccggccag tccgccaatc actttcaaag tagaaagtcc ttttgttgat
     7321 aaatataaaa aggcaagaaa tataataagt gaaataactg ctaccatttg tactgacatc
     7381 gtatttacga tatttccatt tccttggact gcccaaccgc cagcaatcag gattgcttga
     7441 ggcttttgtg ccaaataagg aatatggacg acccagtaag tccaagcggc atagtatgcc
     7501 agtcgtttat tagatgtcgt attttcgacc catgaactga cgcctccttt actatccttg
     7561 aaggctgacc ctaattgtcc aacgatcaat gcgtatggaa taaaataaat ggccaaaatc
     7621 aatagccaag aggtgacaac tgatactcct tgctgagcat agttattaac tacattcccc
     7681 atgccccaaa ccatgttaaa tgcaattaac gcaacagtaa accagcgtaa ttgtttgttg
     7741 ttcattcgtt ctctccttta tgataaaaat ttgttatcca gatgtaatca tattcaattt
     7801 ttaacaaact tacaagacag tgttatttta tgtgtgtatt cttttattta atacacatcg
     7861 ttttttcata ttttttccat caaaaaaata agttattttc ttttatttca tttaccttat
     7921 tgaatttcaa gctattttga attttctgca aaattaaaaa cattagttat tttataatcc
     7981 tatctatttt ctttacattt ttcattaaga aaaggatata aaaaaaagag gacgttcatt
     8041 cgtcctcttc attgatcaac gtttattttt tacatgcaag cttcgcatgg cattcaaaac
     8101 agctaatact gtcaccccaa catctgcaaa tactgcatct cccattgatg caaaaccaaa
     8161 agcacctaag gcaagtacca aaatttttat cccaatagca aagacaatgt tctgtttgac
     8221 gattttcaat gttttctttg ctaaatggat tgcttctgca atcttcgatg gttgatcatt
     8281 catgatgact acatctgctg cttcaattgc agcatcgctt cccaatccac ccattgcgat
     8341 tcctacatct gctcttgcta gcaccggtgc atcgttgatt ccatctccga caaatgcggt
     8401 ctttttattg tttctttgta agatttcctc taaccgctgc actttatctt ctggaagcaa
     8461 ttcgctatat atcttgtcca cgcctacttg cttcccgata tgatcagcaa tttttttcga
     8521 atcgcccgtc aacatgactg tgttcttcac accagccttt tttaattgaa ccaatgcgtc
     8581 tacggcatct tctttcaagg tatctgcaat aacaagataa ccactgtaac tttgatcgat
     8641 cgctagataa agcaatgtac cgatttcttg acttgaagga aaagagattt caaatctttc
     8701 catcaatttg tgattacctg ctaatacttg tttgccatca acttcagcta agatcccttc
     8761 acctgcaaat tcttggatat tagctgttgg taaaagcgga cctgcgtagc cttctaaaac
     8821 agattgagca atcggatgtg tagaaaactg ttctgcactc gcaacatatt gcatgaatat
     8881 ctttggatcg atttttgtat cgatcgtctg gatcgaaaag tcacctttcg ttaatgtgcc
     8941 ggttttatcg aataccactg tctcagtttg tgcaagcaac tccaagtaat ttgaaccttt
     9001 tactaataca ccgattttac ttgctcctcc gataccgcca aagaaactaa ggggtacgga
     9061 gatcactaat gcacatggac atgagatcac taaaaatgtc aatgctcgat agatccattc
     9121 agaaaacgcc gctccagaaa caacaagtgg aggtaaaaca gctaataaaa cggccaatcc
     9181 tacaacgact ggtgtgtaat agcgcgcgaa tttagtaatg aaattttcag ctggtgcttt
     9241 tttactgctg gcattttcta ctaattctaa taatttgctg atagtagaat tttcataaga
     9301 tttttctact tgaactaaaa atggtttatt tttattgata aatccactta atactgtttc
     9361 accaacatat acagatttag ggactgattc tcctgtaagt gctgacgtat cgactaatga
     9421 ttctccttcg atgatcgttc catctaacgg tacacgttca ccaggcttca caagtacaaa
     9481 ctgaccgatc tgcacgtctt cagggttcac ggtcatcaat ccttccgtcg tctgcacgtg
     9541 agctgtttct ggacggatcg ccatcaattc gcgaatcgat tttcttgaat gactgactgc
     9601 caatccttgg aaatactcgc cgatttgata aaatagcatc actgctacgg cttctggata
     9661 ttcaccgatc aacatagcgc caatcgtagc aacagacata agaaaattct catcaaagat
     9721 ttttcctttg acgatattca tcactgcctt cttcactaca tcatatccta tcagcagata
     9781 aatcaataaa taccctatca agcgaattgg ttcattaaaa tcaaataata gcaataaagc
     9841 gatacctgca agggaaaata aaatccggaa cagcgtattt ctctcttttt catttccatg
     9901 ttcagatact ttttccttat ctatttctgt tactttgaca tctggttcta attccttcac
     9961 tacttttttt atttcttgtt caatcgtagc caaatgatct tcctctgcgt caatcgttag
    10021 ttttccagaa gtgaagttta ccgttgcttc cttgactcca ttgatcttct gaactccttt
    10081 ttcgattttc attgcacagt tcgcacaatc taaaccttct aatctgtaac ttttcatttt
    10141 aatgacctcc tcgcctcaac atatgaatat ctcttcataa ctaattataa atgaacggtt
    10201 attcatatgt caaggtatcc gaaagaaaaa atgaaaaaca tctgcatcgg aaaatttctt
    10261 ccatacagat gttttggctt attctttctt tttcttttcg atcattgcca ctgctcttat
    10321 ccaacccgca cttgccttct tgatcttgac aggatagcaa ctaagtttga agcctgttgc
    10381 tggcaatttg tctaaattca ttaatttttc gataggacaa tatctttttt caatccctgc
    10441 aaaatgtcct tcccagatat tcgaagctcc actacgcttt caaaaatata gaaagagttc
    10501 tttaatgaaa attatttcaa ctttccataa acagtaaccc catataaata aaagacgtaa
    10561 aagtcacatg gaagaactta tacaggagaa cgcatagagt caggtgaaat acatgtatcc
    10621 cacctgactc tatgctttat ctaaccttta ttattctctt ttatacaatt tctgcaccga
    10681 atggcatcaa cgccaatcga gcaagtttga aagattgctt ggcaaaaggt atccctatga
    10741 tcgtgataca tagaaggatg ccgctgacga catgtgcgag agcaattggt aggcctgaaa
    10801 cgatcagcca gatgatatta acaatcaatg acatgccaga ctggtgataa attacttctt
    10861 tgccaaaagg cgcaaaagaa agggaggcga tcttaaaaca ttgcataccg attgggatac
    10921 ctatgatcgt gatacaccaa agtatccccg ctgctaacca gctaagacca ccgattagtc
    10981 cgccgaatat aaaccagatg acatttccta aacaactcat tgtatttctc cttctattac
    11041 tgtttttgat taaaactggg caaaaaatga ttttattctt ctttttttag cttccagtat
    11101 caaatattct gcaaattttt tttggatttc ttccgtattt tcttttgtta ctaatgtgtg
    11161 ttggattagt tttcctgatg gtcgctgatc caatcgttct tctgactgtt gcacgtccgc
    11221 ttgcgcaaat ccgataatcc gtttttcttt tgttacagca acgagcaata gtttattctt
    11281 atcatttagg tcagctgccg tttttgcttt tacgaatgat tctggatgat cagtaatcag
    11341 ctgattgatt tgaggtagat cttcttttac tgcccaccgt atttccatca tttctcactt
    11401 ttctttcgct catttttatc tccactaaaa aaatcgactg cggcaattgc atctatccag
    11461 ctttgtgcaa tgatctcatg tccgccaatc gttgggtgga ccccatcttc tcttgtatat
    11521 ttcgaaaaac cataaacaat ccctaaagca ttgaatatac catctaaagg aaccagcaat
    11581 gcgccatatt ccttagcaag tttacgaaca atttggattt tagggtctaa gtctttacgc
    11641 cagccatgac ggtcgtcggt tataggaagt acaaacggct ccatcaagat gatgttgcag
    11701 attcccactg tttgaagttc atttagtaat tttcgataaa cggattcaaa ttgctgagca
    11761 gatttctcag ttccgaatat agcctgatcg ctagtattat gccaggtatc attgattccg
    11821 atcaaaatcg atacatagtc aggttgtaaa gctaaacaat cttcttgcca gcgtacttgc
    11881 agatctgcaa ttttgtctcc tcctatccca cggttcatgc attcaaattc catcgttgga
    11941 tattgggaga ttagttttcc ggcaatcaaa agtggataac ctttacctag atcagttgct
    12001 tctgctcggt ctcttcctgc gtctgtaatg ctatcaccga taaataatat acgttttttc
    12061 atttgtcaaa aactcctttt tgaataaaac gatgattttc ttccttatat tatcccataa
    12121 gccctatcaa gacgaaagag aaaatatatg tataaaaaat gacaccagcc tagtcccttc
    12181 aaacaattga atgaacgctc atcaagtgtc tagtttttta tgctattttt ttcttaacgt
    12241 gccggattcc ccttgattcc ttcgttgttt ggtccagaga gatcgatcac acctaagctt
    12301 ccgtcaatca gaccactgat gacgccatca gaacatatat ttataggctc atctaagact
    12361 tctagtaaat gtccctttac taatatctct tctagattgc ttgggataga ttggtcataa
    12421 ctttatctgg tccgatgatt tcaagggtga attgaactta catatggatt attgcatgct
    12481 gaaaatacgg acctactcca tcttcgaatg ttactattag tttgttttca ggtgctagat
    12541 gctgctttat ttcaacagca gaagcatcat caaatcagat ttccatttga attaccttct
    12601 ttgtaaatgt ttgattgatt gcgatttcgt tatagaacct tgtgaatcaa atagcaattt
    12661 cttttgcttt cttctcttgt ctgctggaaa cttcctttat cccgtatttt aaaaaaagaa
    12721 ctgcgagagt tcgcagttct tttttagaat aaacggtgct ctccggccaa ctcctcggtc
    12781 acaagtcaca atagtcatca aacatgaaaa actgtttttt gaaattactc cttgtgactc
    12841 ggagctgaac ggcaggcgca caacctcttg atataaacgg tgttcaatca gctgaccctc
    12901 gatattagct aaaaaaacgg taaaatatga gaagctattt ttccgttttg tttctctaat
    12961 actcaggtca aggcactgat ttcacaacct cttccttaaa cggtgttcaa aagccagttt
    13021 ctcggtatta agccaaattt atgcaaaaat gtggaaagcc atttccacaa aatttttctt
    13081 aatactcgaa actaaacagc tttttcacaa cctctccact aaacggtatt caaaagctta
    13141 catctgcggt aataagtccc gccaattcaa aaacatgaac agctgttttt gcttggctgt
    13201 tctaattact tgatgcagaa cagctttttc atcacctctt atggataaat tcggttcaac
    13261 aaacgtggga atggaatagc ttcacgcaca tggtcgatac cagcaatcca agtgactgtt
    13321 cgttcaaggc ctagaccaaa accagagtgc ggaacagaac cgtatttacg tagatcaaga
    13381 taccatgaat attctttttc atccaaaccg tgattacgga tttgttctag caagaaatca
    13441 tagtccgttg cccgttcgct tccgccaata atttcaccgt aaccttctgg tgcgatcata
    13501 tctgcacaaa ttaccacgtc ttctcttgtt ggatgtggtt tcatgtagaa tggcttgata
    13561 gcttttggat agtttaggat aaagacaggc tgttcgaatg aattcgcaat gaacgtttca
    13621 tgaggtgaac caaagtcttc gccccattcg atgtcatcaa atccattttt cttcagtaat
    13681 tcgatcgcat catcataagt aatacgaggg aatggcaatt ttgtataagt ttttaagact
    13741 tctttgtctc gtcctagtac atctaatgcg tattcgcaat gatctaaaac attttgtact
    13801 aagaatgcta catattgttc ctgtacttct aaactttctt cttgatgagt gaaagccatt
    13861 tctggttcga tcatccagaa ttcaatcaaa tgacgtcgtg tttttgattt ttcagcacgg
    13921 aaagtcggac cgaatgaaaa gacttttccg aaagccatag cagcagcttc catgtataat
    13981 tggccacttt gagataaata agctttttga tcaaagtaat ttgtttcgaa tagatccgta
    14041 gtgccttctg gtgcggaacc agtcaagatc ggcggatcga ttttgacaaa atgatgttga
    14101 ttaaagaatt catacgttgc acgaatgatt tcatttctga tctgcataat cgcatgttgt
    14161 cttgaagaac gtagccataa atgacgatga tccatcaaga agtctgttcc gtgttctttt
    14221 ggtgtgatcg gataatcgtg gctctcgctt accacttcca catttgtaat tccgatttca
    14281 taaccaaatt ttgaacggct gtcttcacgg atttctccag ttactaacac ggatgtttct
    14341 tggtgcagat ttttagctgt ttggaaaact tcttccggca cttcgctttt tacaactaca
    14401 ccttggaaaa aggctgttcc gtctcttaat tgtaaaaatg cgatttttcc gcttgatcgc
    14461 ttatttgcga cccaagcgcc gattgttact gtttctccca catggttttt cgaatcgatg
    14521 atttgaattt gttccacgta tgactctcct tttatattcc ttgaatttct tcaggattaa
    14581 ttttgacttt tactctcaat aaattgcttg attctgcgta ccgcttcttc taacgtagcg
    14641 cgatcagtgg cataactcat gcggacattt tctggtgccc caaagccttc gccagtcaca
    14701 agcgctaccc ctgtttcttc taaaagatct tctacccatt gtgtgacatt ttcatattta
    14761 cacatgcgta gcgtttcttt tacattagga aaaagataaa aagctccttg tggcttttca
    14821 agagaaacac ccggcagttc tgccacaagc ggatataacg tattcaggcg ttcttcaaag
    14881 gcgttccgca tttcttccac tgtatcttgc tcgcctttca atgcttcaac agctgcatat
    14941 tgactgactg ctgtcggatt gctagttgat tgggaagcaa cagcgatcat tccattgatg
    15001 atcacaggat ttcccaccgc ataaccaatg cgccagcctg tcatcgcata tgttttagac
    15061 acgccgttga tgatgatggt ctgatttttg attgcttctg aaatcgttgc aattggagta
    15121 aactcattgc cattataaac taaacggcca taaatatcat cagcaactat caaaatatca
    15181 ttttttactg cccattcacc gattgcctgc agttcattct tgctataaat cattccagtc
    15241 ggattcgatg gagaattgat gattacggct tttgtttttt ctgaacgtgc ttgttctagc
    15301 tgctcaacag taactttcca gctattggat tcttctcctt tcaaaaagac aggaagtcct
    15361 tctgccagtt ttacttgctc accgtaactg acccagtaag gcacaggaat gatgacttca
    15421 tcttgcgggt caaggatcgt ttggaaaagg gtatacaaag caaatttcgc tccgtctgtt
    15481 acgactgttt gtgaaggttc atagacaagt ccatagtttt tcttcagata atcaacgatt
    15541 gcctgtcgaa gctctgggat tcctgctgtt ggtgtataat agctagcttt cccattgcgg
    15601 attgcttcga ttgctgcttc ttggatgttt tctggtgtcg caaaatccgg ttctcccaca
    15661 gtcaaactca gcacatctct gcctttggct tttaatgcct tggcctttgc cgcagctgcc
    15721 aaagtgacag acggttctaa tcgttttgtt ctttttgata actccatccc attcattcct
    15781 ttacctttta aattcccttg attgtcttga cttcatctcc agattcaaag ccaatcaggt
    15841 aataattcag tttcccattt gtttcttttc ctactacttc ccacacaggc tgatcatcat
    15901 acatccctaa agcagctttt tcgatttgga tttcaggatg ggccgcagca attgtctgtt
    15961 ttgcttcatt ttcagtcagt ccctttgttt gatccaatac ttttactttt tcaccagatt
    16021 taggaatgat aacggcgatt tcttttcctt tatcatttgt acctgtcagt gaaaagtatg
    16081 tcttctttcg agtaaaccaa taaaaatgat cggcttcctc aagatttgca tatttctttg
    16141 cgatttctac tgcttctttc ttcgcctgca ccgccggacg actggaacgc aagtaaaaaa
    16201 tcgagctgat cacgatgacc gctaataaaa atacgatcaa gccaagtaac acgcgattaa
    16261 tcgttttttc ctttgtctgt tttttcttca agggtcgtcg ctcctttatc ttcattcctt
    16321 ttcattatag cagattttaa ttatgaaaaa agacagctcc tctaacttcc ttcttcattt
    16381 ttctttaaga attcatggat atcctgatag atttcctcca tagctagctc tttgatcggt
    16441 acttttttcg gtaatgcatg gatgatccgt gcaccatatt ttgcagtaac tagtcttcga
    16501 tccaaaataa gcaatacgcc acgatctgtc tccgtcctta tcaagcgtcc taatgcttgg
    16561 cgtaacttca aagctgcttt aggtacggat tcctggtaaa aaggattgat cccctctgat
    16621 tctaaataag cgttcctagc tttgaccatc gggcgaagcg gattatcgaa gggtaaacgt
    16681 gtgactacaa cgatctgcaa agcatctcca ggcaaatcta caccttccca aaaactgtca
    16741 gcgccaaaca atacgctttt ttctgacaaa atgaagcgtt tcaaaagctt ctcccggctt
    16801 ccgccaaacc cttgagctag tatttctcgt cctttttcta gcatagacag atgcattttc
    16861 tgatagacct tctgcaaaac atcatgagaa gtgaataaca caagtattgg ccgatcttct
    16921 tgttcgatca gacctgtaag tgctgtctct aaataatcga tatatgcttg attgctgact
    16981 tgatggacgg caggtgcctc ttttggcaca tagatccttg cctgtttctc ataatcataa
    17041 ggagaggcaa tcactttaag aggtgctgct tcaatgccaa gccgcttaga gagatagtgt
    17101 cgattagggc cgatcttcag cgttcctcca atataaagaa tacgatcata tcgaggatac
    17161 caaacagtcg acggcaacat agccgcttga aaatcatgga gatgaaaaat catctgctgc
    17221 gctgtgttcc caggaacgaa ccaatggatc aaatgatctc gccagtcttc cagccattga
    17281 tccatcagca agtactgctt gtctaattga tcaaataagc tatataaact tgctaaatcg
    17341 atccgcatct ggtttgtcca gctttcttgg ataatctcaa aagtcagacg catttgtgac
    17401 tgtagctgct ggatctcctg ataaaaaagc tgcaggtgat caatggctgt cccagattcc
    17461 agtggcagct gatccaacat ctcttttgtc gtcatgactt cttctggata ctgtccgtta
    17521 ggttgcttgc cgctcactaa actcattaat ccttctgcca tacaaatctg tgcttcgcat
    17581 agcgcagaaa gttcttctcg ataaatcgat aaccagcgta aggcttcatg ttctttcggt
    17641 aaatagcttt gtattcggtc aaacagctga tttggttctt ctaattgctg gatttgttta
    17701 tggaaatagt taatacttaa tcgtttgtca tttacttttt cagcgatttc cggcaaatga
    17761 tgtacttcat cgatcaacaa aaatggacta gctggaagca atggttcttt tcgctgtgtt
    17821 tcttgtatta aaaacgcatg attgacaatc aaaaaattac tttgcctgat tttcttttgc
    17881 aagaaacgaa caaagtcttg ttcgtaaaat gactgatttc ttgctaaaaa tccagtgcct
    17941 ctgtgcgcga tatctgcaaa gaatgaatgg ttcaaatttg ttaaatgtaa ttcatccaaa
    18001 tcaccggtct tcgtttgcgt cagccaaact aagaccgcca tttgatacaa agcatattgt
    18061 ttttgttctg gcggttccac aagtgtgcct ttaaaacgct gtaaatcaat atagtggcga
    18121 tagcttttga taattacagg ttgtatgggc tgatctagca attgattcag caatggaatg
    18181 tctttttcta tgatctgctg ctgcaaaact agcgaaacgg tactgatgac agcgggtttt
    18241 tcaggtgtag ccaaataact caatggcagc agataaccga ttgttttacc cattcctgta
    18301 gctgcttcga tcatcaaatc ttttgattca tcttttgtga aatggtcgta gaccgcattc
    18361 atcagtctgt tctgttcttt tcgatacatc agtgttttac caaacatttt ttcttttgct
    18421 tgtttttttc ttggatatgt ccgctcgcca taatggacgt atatgaatag ttccacttct
    18481 tttttctgca aagctagccc atcgacgatt tctaatgagg gatcaagtgg ctctggtttt
    18541 tctttcattt cttcaatcac atgataaatg aaacgactcg tatccatacc tgtgtgtcta
    18601 cttaatcgcg caatctgctc taacgtgatg attggcaatt ttctcatccg ttcttcgatc
    18661 agcaaaagaa gttgacccgt cacttcggca tcactatctg cctgatgagg attctcatga
    18721 ataaatccaa ggctttctga caaatcagct aagcgaaagc ttggttccgt cggcaagaaa
    18781 atttgtgcaa gttctactgt atcgatgcct ggtatcttca aactaggaag tccgcatcgc
    18841 atgagttcat ggttcaaaaa attataatca aaataaatat tatgcgcgac aaatatggtg
    18901 tctgccaaaa gattgtaaat cgtttgggcg acatcttcga aataaggcgc tttttggacg
    18961 cgttgattcg tgatccctgt taaatgctgg atttgttttg aaatcttccg ccctggatga
    19021 atatcgatcg aaaaacgtga agtgatccga cctgattcaa tcatgacgca gccaaattgg
    19081 atgatccgat cttcttttgg attcgtccca gtcgtttcga tatcaacgac tgcataagtt
    19141 cttggtttca aaatatcttc ctcttctcct gcctctaatg ttcttttgaa attatactac
    19201 aaagtgcaga aaaatactct catcgctttt taaagaaaat agacaaaaac gcccagacac
    19261 gcaaatgtct gggcgtttat tttttagtat aggttaatta agctttagta acgtttgttg
    19321 cttgaggtcc gcgttggcct tcttcaacat cgaaagttac tgcttggcct tcttctaaag
    19381 ttttgaatcc gtcgccttgg atagctgaga aatgaacgaa tacatcgttt ccgttttctg
    19441 cagtgatgaa tccaaatcct ttttctgagt taaaccattt tactgtacct gtttccataa
    19501 taaaaatcct ccttgtgtat caacacatat tttgcaatta cttgaatggt gtatatctgg
    19561 aagatgttta tttgaaacaa gctaccacag attaccgaac aaaaactact gctatagtat
    19621 atcataccgt ttacaaaaaa gctagagttt ttcgactttt tttattaaaa aaagttttct
    19681 cagcgattac ataaagtgaa aaaccgaact ttttttaaaa taaacgacat attgttcagt
    19741 tggtgttttt ttgctctgat gttcttcttt aaactaaaaa aattattctt tctgactact
    19801 tatcggttct gtttcaacgg tcgaggctgg ggaagattct ttagtaggtc tgatgttctc
    19861 atggtatttc tgatagccta tgacagttac cagaagaaca aggcaaacca atacgatcaa
    19921 cgagattagt ttttcttttt tgctcatatc tatacctcat aaataacagg atgatgtcca
    19981 tcatatttct gagtatgagt aaaagcaaaa gctgcagctg tagaatgtaa gaaatgccag
    20041 caactttcat aatcacaggt cggacagtac caaaagtaac caaatcttgg ctgcaaagac
    20101 cgttcttctg tttcaggaat cgcagaatca agatctgttg tctgtacata aacatttttg
    20161 tacagacgtg tgcctgtaat aggagaccgc tgtgtttctt cagcaaaaac tggctgactt
    20221 gttaaactta gggatagaaa cagcaaacac cataacagtc tttttttcat aaagacctcc
    20281 ttatcgataa gtttttccca attaaaatca tatctcaaga acgcccgtta ttcacatttt
    20341 gttcataaat gatgtttttt gtatcataat cgtcaaaaag aggtcgtgac cacgtcacaa
    20401 cctctttttt aaacggtgtt ccagaagtag cttcttcgga aataagtcga aatttcacaa
    20461 aaatttgaga aataattttt gtcaatctct tcttatttct tgaagctgac cacttctgtc
    20521 acaacctctt ccttaaacgg tgttcaaaag cttgcatctg cggtattagc tcaaaaaacg
    20581 gaaaaatatg tgaaactatt tttccgtttg tttctctaat actcaggtca aaacactgat
    20641 ttcacaacct cttttaataa cgaagaatcg ctgtcagact cttttcatct ggcgcatctt
    20701 tttgatccaa aataaacact ttcccgccat taatgatcac atcatctgct aatgcattca
    20761 acacttgacg ccgatcatat tctgtttcag gatcttcacc gaaaccatca gctaagtttg
    20821 acgtagcgat aaataaatgt gacacttttc cttctttcgc agctggaacg atatcaacta
    20881 actgatcgat gaatttctta tctaagagtt tattgtattc ttttgattct tgagctgcga
    20941 gttcacttga cagcttcttc gccccttctt cgatatctct aaaggatagc tgcgcaggag
    21001 aagcagaaat cgcaacgtct gcacaataat agggattttt agcaattttt ttgaacatcg
    21061 tctgattttc tggtaatgca aataaataaa tcggccattg ttcaggattt tcgaaagaat
    21121 ccttcaagta cgtatcaaca gcttggtaat agttgaccca atcgatctct acttcttcat
    21181 cttttggatt tacgccatgg aaagttactc catctcgggc tccgcctcct ccattagaag
    21241 agaagttcaa accgccacct gtcagttcag atcccaatgc agtaacgaca tcaaccggtg
    21301 ccccttctgg tagagatact tcttttactt gtttgttgct gacctcatac aatttcatgg
    21361 agtcgcggtt caatcccaat aaataataag aatagttgaa ctgcccattt ttgataatag
    21421 ccagtaaata aggaagatca cttacatagt attgatcgtc taccgggata ctcaaacgat
    21481 ggatataggt ctggtcagca gttataatga ccgctacact cgtagttgca ctacgccaaa
    21541 acgaaggatc tcctaacaaa ttttcaattt tttcttggaa cggggaccag tcatgttcgg
    21601 aatatttttt ctcaaaacgt ttctttgctt ctttcgcaaa atttttgaat ttgatctgat
    21661 ctttttccac atcttgatgg gcgacatgcg tattcagcat gatcgtgacg aatggtcctt
    21721 tagcgccttc tgaagtcaat tgtgtcaata attctttatc cttttttgcc ataaacgtac
    21781 ccctcctaaa aaaatcattc ctttgtttct atctaaagtc taccacaaca ctatttttcc
    21841 atacaataaa acgtatctaa aaaaataaaa cggtttctac ggttttaaat ttagctattt
    21901 ttttaagtta tcttcataat ttcctcaatt atttagcccc agtcttcaac tattcttcat
    21961 gaaatgctga taaactaaga aaaagaataa aagaggtgga tcttatgcat ttgattatga
    22021 ttttcagttt attaagtatt atcagtttat ttgctttcag cgcttcccgc ttattagatg
    22081 cggaaacaca gcctattcct gtaaaagtac gtaaaaacga ccgctaaact tccataacgc
    22141 gcgttcttat cctaaagttt tttcagaaac attgaaactc tctcccctct ctgataaaat
    22201 tttaatttgt agcgaaacaa attaattaca aggagatcgg atatgtccat aaaaaaaaca
    22261 cgtctagtcc tattattggc tagtctcatt ctttgccttg cttcttttct accagctgtt
    22321 gctgtgcaag ctgaagatac gttcaaagta aatgcaaaag cagcttttgc tgtagatgcg
    22381 caaagcggta agattcttta tgatcaagat ggagaaaaaa ccatggggat cgcttcaatc
    22441 acaaaaatca tcggtcttta catcgtcctt gatcaagtaa aagaaggaaa actgtcatgg
    22501 gatgataaag tttcgatttc agattatgca gaaaatttga gtatcacccc agatttatcg
    22561 aatgttcctt tgcataaaga aaacacctac actgtcaaag aattattcga ctctgcaatc
    22621 atccagtcag ccaatgcttc aatggtggcc ttggcagaaa aaatctctgg cagcgaagct
    22681 aaattcactg aacgaatgaa agaacaattg aaagattggg gaatcaaaga tgcaacaatc
    22741 gttaatgcat caggattgaa caactcttat cttggcgaaa accgaccaga aggcactgga
    22801 gaaaacgacg aaaatcaaat gtctgctcaa gatgtggcca tcgttgcccg ccatttgatc
    22861 ttggatttcc cagaaatact ggatgtatca agtactacga cccaaatgtt tggcgaaaat
    22921 acgcaatcac cagtggaaat ggtcaactgg aactggatgc tcccaggatt cgttaattac
    22981 aaggaaggcg tggacggatt aaaaactggt acgactgagc ttgcaggtgc ttgtttcgtt
    23041 ggtacgatca ctaaaaacga tcaacggatc atcactgtcg tattgaatgc ggacggccat
    23101 gcggaaaacc cgagtgcccg cttcaatgaa acagaccgtt tgatggacta ctgctatgac
    23161 aattggtcag aaaaagagct aggaaaagct aacgcaagta ttcctagtct aaaaaccatt
    23221 gatgtaaaag acggaaaaga atcaacagtt cctgttgtgc ttaaaagccc tgtaaaagta
    23281 tgggtacgaa acgatatgga tactggcaaa ttaacgatca cgccaacact tgacaagaag
    23341 caagtcactg acaatgagat ccaagcacct atggataaag gtacaaaaat cggtaccgct
    23401 acgatcacct tggcagacga taaattaggc tatttagaag acaacaatgc accttcaaca
    23461 gagatcatta cggataaaac agtggaaaaa gcgaatatat tcgtgattgg ctggcgacgc
    23521 gtcgcagaat ttttcggcaa cttgttttaa ttgaacaata agaaaaacat ctttccatcg
    23581 gaaagatgtt tttttgcttt ttctagttta aataacagct tttttcatct attttcgtta
    23641 tactgtataa gaaaaaggag ttgaatttat gaatctaacc agagatctga tctttttagt
    23701 tctctatctt attggggtat tctctatcgt ttattggcga aaacagaaga aggatccttc
    23761 cttgttcttt tacggcagta cgtggacatt tacggtaatc ttaacttatt ttgtcgtgtt
    23821 ggaaattttt tattcgacct tttatttttt gattccattg ttgttcttta gtatctttgc
    23881 tttttcttat ttcacagaaa aacgaaggct gctgaatggt cttttattca atgttttcct
    23941 catcagtttc ggcatctact tgtttgtctt gctgtatgaa acgcaaaata ttttccttgg
    24001 cggactgatc gccttgatca cgattccatt gctacttgtc cttttatttg gtatttatgg
    24061 attgattgtt tttctttttt ggaacggggt cacagtttta cgtagagaat cgcattcatt
    24121 agctaatctt ttaacgttga tactagccat ttttcttaca ctgtttcttg tttttgactt
    24181 tttcttgtta aaatatttgc cgcaatggat aaatgctttg tttttctgcg ttcccctgat
    24241 cttgatctat ctatttatcg tattctacaa ctttttgact gtctcattct tgtatcagtt
    24301 caaccggcct cgatacaatc aagacttcat cgttgtgtta ggcgctgggt tgatcaatgg
    24361 agaaacagtt tctcctcttc tagccaagcg aatcaataag gcaattgctt tttatcgggc
    24421 tcaaagccgt gctacattaa atccgcccat cttattgatg tctggcgggc aaggagcaga
    24481 cgaaaaagta ccagaggcca ttgcgatgaa acaatatgcc atggaacaag gaatccctga
    24541 aagagacatt cttgtcgaaa ccaattcgac aaccactttg gagaacatgc tctattcaaa
    24601 ggaaatcatg gatcagcaaa tgaaagggcc ataccgcgcg atctttagtt ccaacaatta
    24661 ccacattttt agagccggtc tttatgcgtg gcaagccaaa ttgaaagcaa atggtattgg
    24721 tgcaaaaaca gctttctact acttgcctaa tgcgttttta agagaataca tcgccatctt
    24781 gatgatgaac aaaaaaagac atatgatcat ttgcggattg cttttcgcag gctccgcatt
    24841 cttatcgctg attactttac ttttctaaaa aaacaaggtg tgacaaaaca tatgtcacac
    24901 cttgtttttt tctttcattc tcttaactat tggcaaaaag agtatccctt ttttgaccgc
    24961 tacttgctga gcagaataaa taccgctgtt gcctgaacac ataaagctga tcaagcagat
    25021 cataaagaag tatactgccg cttgtgaacc gaacaattcg atccccatca caaagcaggc
    25081 aatcggtgta tttgtcgcac cagaaaatac tccgataaag cccaatcctg caagaaaagg
    25141 aacagaaaca tggagcaaag gcgctagtgc tgcaccaagc gttgcaccaa tttcaaacaa
    25201 tggtgtcact tctccccctt gatatcccgc gcctaaagac aaaactgtga agaataactt
    25261 cccaataaaa tcatagacat gtgcctgtcc attaaatgat tcttgaagta acggaagact
    25321 caatccgaga taacgttgtg tttgcaaaat caatgctgct gccacaacta cagctccacc
    25381 tatcgtattt cgccaaacag gatgaggaat ccattgtgca tatcgcgctt ttaaaaagac
    25441 gatcgaacgg ctgaataccc aaccaatcaa accaaaagca atcccagcaa caaaaacttt
    25501 gaagaacagc aacatactcc attcaggaat cacacccatt tggtaatgta gatgagaaac
    25561 accatagctt tctgtcatga aatttgcaaa cagtgcagca aagaagctag ggaagatcgc
    25621 ttctgttcgg actttcccga ttgccaacac ttctaatcca aataatgttc cagctaaagg
    25681 tgtgccaaat acagaactaa aacccgcact gattccactg ataatcaaga tttcccgttc
    25741 tgatttatcc aatcggaaca aacgtgctgt attatctgct aacgctcccc ccatttggac
    25801 tgctgttcct tcacggccaa cagatccccc aaataaatgg gtagttatgg ttccaaacag
    25861 tgtaagtgga ataagtcgca acgggatttt ttcttctcca ccatttcctt gatcgatcac
    25921 taagttgttt ccacgactag aaagaccgcc gtgatacgca tagagataag caaaaagcgc
    25981 accactaact ggtagtagat aaaggagcca aggatgtgat agtcggatat ctgtcacaac
    26041 tgtcaggcta tttaagaaaa aagcagacaa gctccccatg aatataccga gaatcgctgt
    26101 gatcaccagc cacttcagca tgtacactaa aatgcgtact ggactcggta attgtttttc
    26161 cataaaaaat tcctcctcaa taaaaaaacg cctactaaag aaaggggctg tccccagact
    26221 ttagtaggcg tcattagcta tctttcgtta gcatttaaag gcgaacacca tcacctattc
    26281 acttccatta aaagatagca tatttttctg taaaaaacaa taaaattatc gttctttctt
    26341 tacacgttca gcaaattcat agaaaggagc caatttcgtt aactgttgat tacattggtc
    26401 actatcatga caaatataat tccccttttt cgtatacgtg ccatcagaac ctgctttcgt
    26461 tgtagaaaga aaaagcgaaa cattcgatgt tttctgacag attgcacaga ttcccttatt
    26521 tatcgttggt gataaagtac cactaatccc ttttaatttt tcatcttcgt aatagatcat
    26581 gaattttttc tgcgtacctg gatcattcca gccgatgtac gtatgttctt gcaagtcgat
    26641 ttcattccaa gctggttgtt tgagtttttt cacttttcgg aataatttct cgatttgtgg
    26701 tttgctaggc tgcataaacg gctgaacgat ttgcttcagc tcctccaatg ctcgttctgc
    26761 ttgcgctttt gtcagtttta cgtctaaaac tgtcgttaaa aatgtatcga tttctggcag
    26821 ttcgtgttct aatatgtctc gaatcttttc agttgctagt gcctgaactg tttgtaccgt
    26881 ttttggatca ttgacagatg agtacgcatt taataactgt tctacttctc tttttataaa
    26941 aaaatattgg tatggtttta ttgtttgttc catgatttta aagtcctttc ttctaaccaa
    27001 agtgcatact ttgtagaaaa aagtgatctt ctgcaaagta tgtgttacct gaagatcccg
    27061 gactctttta aataagatac gttttgcatc ttatcatcac cccctttaat gcttattctt
    27121 ctatgtacat gattcattcg ataaataaaa gtaaaccgta tcttgaacag atagtcaact
    27181 gcaaacagaa aaaaggactg tgacaaaagt cgtgtcaaag tcccttgttt cgaatgaatg
    27241 gtgttcaaaa gcttgcatct gcggtaataa gccgaaactt cacaaaaatt tgagaagtaa
    27301 ttttcgtaaa ttccttctta tttcttgaag ctgaccactt ctgtcacaac ctcaatatgg
    27361 tctattattg gttttacctg ttaaatcgag gattctttcc caaagtaacg ttgccatctc
    27421 ttacgcatac ttgcagacat gggctgagcc gcttctttga ctgcgcagta tttgtccacg
    27481 aatgtcacaa tatatccttc tttatactta ggcggtgcga tcgttgcacc ccacatgtgc
    27541 ttcaaaatga tatctcgctc aagatccgac aaatcggtga ttttttctgc attctttaca
    27601 gcaattcttg gatggatata cgcatgtgtc ccctcgtcaa atttcgtcgt acgccaatca
    27661 tagtagaaca gatcatgcaa aagtccagca cgcgctgttg ctttagcatt gccaccccat
    27721 ttttttgcga gcagataact atagtaagaa acactgatgg aatgctccaa acgagtggaa
    27781 tgaacatgct gtgtgtaatt cgctaatttt ttcactgctt ctgtttctaa aagatcttgt
    27841 acataagaca tatattcttc gtcttcacgc catttttctg tcataattat cgatctgcca
    27901 cctttttgaa tttcattttc ttctgaaatt gatctaagta taccaccagt gttcggatta
    27961 ttctagtcgc tctagataaa atagctaaag cttaatagtt ccttaatcat aaagaaaaat
    28021 gataaagaac gatccctgct gcgactccga cattcaatga ttccgcattt cctttcattg
    28081 ggatatatac gttctgatca gtcaaagaca agatttcctg acttactccc tgaccttcgt
    28141 ttcccatgat tatagcgaaa tcctctgttt tttctacttt gtctaatgca tgtgcttctt
    28201 catttaattc tgtaccgtac accgtaaacc ctttgccttt cattacaggg atcacttctg
    28261 ccaaatcttt ttgtaacaca ggcaaatgat aattgcttcc ctgcatgctt cgcagcacct
    28321 ttgtactata aatatctgcc gttcctttac ctaataaaac accagccata ccaaacgcat
    28381 ctgccgtacg gatcatcgtt cccacatttc caggatcctg tacattatct agtaaaagcc
    28441 agcctcctga aaaatctggc gtatcttcag atgttaacgt catcaccgct aagatccctt
    28501 gcggtgtagg tagatctgac atagcggtca tgacctcatc tgaaactaga aaagtagaaa
    28561 tctctttgtt tacagccagc cagtctcccc attctttcat acctctctgt gtcacaaaaa
    28621 gttcttttat ttctgcatca gacgacgcag cttcttcgat cagatggaac ccttccaaaa
    28681 gatattctgc cttttcttct ctgtatttct ttttatgtag cttttttaat tctttgattt
    28741 ttggattttt tgctgattgg atctctttca cattccttca cctcacctga ttatatcatg
    28801 gaataggtgc ttgagcttgt ttcatcctaa aaaaatcggt atcattgatt tataaaacag
    28861 gaaagaaagg tgacgaacat gcgcaaagtt aaaatgaatg tccaaggacg tgtccagggc
    28921 gtgggatttc gctatatgac caagatggtt gctgatcagt taggcgtgac cggaacggta
    28981 aaaaacgaag acgacggctc agtcacaatc gaagcaatcg gaaatgacga catcatccaa
    29041 aaatttatcg aagaagtcaa gaagtctccg agcccaagtg gacgagtgca gtatgtggat
    29101 gtccaagaag atccgatgat tgaggaacga aagaaatttg atgtggtggg ataaggttgt
    29161 gaaaaggtcg cttagttttg agtattaagg aaaattttgt gaaaatggct ttccacattt
    29221 ttgcataaat ttgacttaat accgagaaac tgacttttga acaccgtcga tcaggttgtt
    29281 tttgaggcgc tttgcctcaa gtattaaaga taggtagtaa aaaagctgat ctataacttg
    29341 caat
//
LOCUS       DAZUMK010000019        29214 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000019 DAZUMK010000000
VERSION     DAZUMK010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.019"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(416..1684)
                     /locus_tag="ACG808_000893"
     CDS             complement(416..1684)
                     /locus_tag="ACG808_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFS5046541.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(1915..2652)
                     /locus_tag="ACG808_000894"
     CDS             complement(1915..2652)
                     /locus_tag="ACG808_000894"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS5046542.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(2719..3288)
                     /locus_tag="ACG808_000895"
     CDS             complement(2719..3288)
                     /locus_tag="ACG808_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFS5046543.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            3416..4288
                     /locus_tag="ACG808_000896"
     CDS             3416..4288
                     /locus_tag="ACG808_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS5046544.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4630..5502
                     /locus_tag="ACG808_000897"
     CDS             4630..5502
                     /locus_tag="ACG808_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS5046545.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            5499..7457
                     /gene="nagE"
                     /locus_tag="ACG808_000898"
     CDS             5499..7457
                     /gene="nagE"
                     /locus_tag="ACG808_000898"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFS5046546.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPTAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(7789..8640)
                     /locus_tag="ACG808_000899"
     CDS             complement(7789..8640)
                     /locus_tag="ACG808_000899"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS5046547.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            8763..9230
                     /locus_tag="ACG808_000900"
     CDS             8763..9230
                     /locus_tag="ACG808_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFS5046548.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(9493..9975)
                     /locus_tag="ACG808_000901"
     CDS             complement(9493..9975)
                     /locus_tag="ACG808_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFS5046549.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(10075..10938)
                     /locus_tag="ACG808_000902"
     CDS             complement(10075..10938)
                     /locus_tag="ACG808_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFS5046550.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            11536..12411
                     /locus_tag="ACG808_000903"
     CDS             11536..12411
                     /locus_tag="ACG808_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFS5046551.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(12556..12708)
                     /locus_tag="ACG808_000904"
                     /pseudo
     CDS             complement(12556..12708)
                     /locus_tag="ACG808_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(12976..14307)
                     /locus_tag="ACG808_000905"
     CDS             complement(12976..14307)
                     /locus_tag="ACG808_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS5046552.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(14456..15169)
                     /locus_tag="ACG808_000906"
     CDS             complement(14456..15169)
                     /locus_tag="ACG808_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5046553.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            15344..16768
                     /locus_tag="ACG808_000907"
     CDS             15344..16768
                     /locus_tag="ACG808_000907"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5046554.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            17067..17774
                     /locus_tag="ACG808_000908"
     CDS             17067..17774
                     /locus_tag="ACG808_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFS5046555.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            17761..18084
                     /locus_tag="ACG808_000909"
     CDS             17761..18084
                     /locus_tag="ACG808_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFS5046556.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(18173..19210)
                     /locus_tag="ACG808_000910"
     CDS             complement(18173..19210)
                     /locus_tag="ACG808_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFS5046557.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(19531..20514)
                     /locus_tag="ACG808_000911"
     CDS             complement(19531..20514)
                     /locus_tag="ACG808_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFS5046558.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(20929..21699)
                     /locus_tag="ACG808_000912"
     CDS             complement(20929..21699)
                     /locus_tag="ACG808_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5046559.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMIFGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(21692..22627)
                     /locus_tag="ACG808_000913"
     CDS             complement(21692..22627)
                     /locus_tag="ACG808_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5046560.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(22943..24592)
                     /locus_tag="ACG808_000914"
     CDS             complement(22943..24592)
                     /locus_tag="ACG808_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFS5046561.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(24709..27312)
                     /locus_tag="ACG808_000915"
     CDS             complement(24709..27312)
                     /locus_tag="ACG808_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFS5046562.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(27324..28373)
                     /locus_tag="ACG808_000916"
     CDS             complement(27324..28373)
                     /locus_tag="ACG808_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFS5046563.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(28399..28713)
                     /locus_tag="ACG808_000917"
     CDS             complement(28399..28713)
                     /locus_tag="ACG808_000917"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFS5046564.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(28729..29175)
                     /locus_tag="ACG808_000918"
     CDS             complement(28729..29175)
                     /locus_tag="ACG808_000918"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFS5046565.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
BASE COUNT         8818 a         5924 c         5032 g         9440 t
ORIGIN      
        1 tcaaagaatc agctgtgtaa taaaaaaaac gctctcaata gaaaagtata gagaaaagaa
       61 attttttgta gaatgaacgg aaaaaatgat aaagaaagca caacgctaaa accaagatta
      121 ttgattctta tcaaactata gaagatagct cttctgcagt agataatttt acaccgccaa
      181 aaatttccga aatcgccttt tgcaagaatc ccgtcgaata ttagaaattg gatcagtatg
      241 cttaaacagt aataaaaaag ttatgacaag atgttgtcac aacctttttt gagtaaacgg
      301 tgttcaaaaa cttgcatctg cggtaataag ttcaaccaaa caaaaaacat gaaaatctgt
      361 ttttgtttgg ctgttcttac tacttgatgc agggcagctt tttcactacc tctttttaat
      421 aaccataaaa tcctattcct tcatatctcc agcccatcgg tgttagccaa tctctttcct
      481 tagcactcaa ggtatagtga tgggtaccag ttttttcatt tggattaaat agtcgataaa
      541 ttggcttgga accaccagaa taaaaactta gaccttcata acgccaaccg atttttgtta
      601 aagagttgat ctcagattgt gccattgtgt aatgatggtc tccactgtaa ggattgtata
      661 aacggtagac aggttgtccg ctgtttggtg cttgccaagc aatcccttca tattcccagc
      721 ctacagttgg cagataattt ttttctccgg cattcaatgt atataaatgt tctcctgtat
      781 gacggttata cagacgatac atcggagaag ttgaagttgg tgctgtatca tactgtgtca
      841 gattataggt ttcgattaca ttgttcaatg tagaaccata attaggcgca gtggcataac
      901 gacctgttag ccaagccgta gcatctttgt aagagttcgt attgcttttc catgcaccgg
      961 aatagtagta aacgcctggc tgaaaagaag tcgtcttcaa taccgttacg ttatcttgca
     1021 atgattcttt gtacgaagga tattttctaa aagcggcatt caccgtaatc cattgcccat
     1081 tcacatattc cgaagtaggc atcgtaacag attggccatt atagcttcct ttaatcccaa
     1141 atagattgta attaggggca gaagccaaag tactgttccc ccagccactt tcaacgatgg
     1201 cttgagcaat catgactgat gcatataagt cattggcact tgccaaactt tgtgcagttg
     1261 aggcaatact atcaatgaac gcttgttgag aattagcaga tgagcgagca taaaaagcat
     1321 agccagtata gttttcgaat tcagacaaat ctgcttgatg ggtatgtgtc aatgtttgcc
     1381 catctgtcaa ttggttcgtg atttcttctg cattctctgg attgttctga gcttctgctg
     1441 atgtactgtc ttcggtttgt gtttgttctg ttgtttcagt tgttggttct tcggttgaaa
     1501 ttgtattttc tgagacagta gctgactgca aagtactttc tgtatcgttt tcaattcctg
     1561 tttctgaaga actcgatgtc tgagttgcga cagtttctgt cgattcttca gctagcactg
     1621 ttgtgttggc aactgctaga tttcctaaaa gaataacgct aaataataaa ttctttgctt
     1681 tcatcttttt tctcctttga attatgaaac ttttcctaaa taacagtgta actaaaaaag
     1741 taaaagcaag tcaatgtcca tatttaaata acatttgttt ctttttaata aatttgcttt
     1801 ttctgcataa aaagacgtaa atgaagaata ttcttctcca ttcacgcctt aaattgatgg
     1861 tcgtttgaat aactatttaa caagtaattc tctctggata ttatccgtta tggattatac
     1921 ttcctgtgtc gtcggacgga taatcatgtt gctgacagac atccggtctg gcgtttctat
     1981 cgcaaatgct acagcagaag ctatatcttc agatgttaat ccccattctt tttgcgcttc
     2041 atgaagttca agagcagcct ttttgtcgct gatggtttga tataattcag tttgaacagc
     2101 tcctggagag atgatcgtag atttgatgtt attttgccgt tgctcttgac gtaatccttc
     2161 catgattgca cggacagcaa attttgttcc gcaataaacg gcggaatcag gataaacaac
     2221 atgtcccgcc acagaatcag ttgaaatgat atgcccagat ttttgttttt ccatgattgg
     2281 aagtacggcg gaaattccat ttaacacacc catgatgttg atatcaagca tattttgcca
     2341 ttcatcacgg tgaccttcaa ctaatggagc ggttggcatg attcctgcat tattgtacaa
     2401 tacatcgatt cgaccgaact tttccatcgc taaatcgata actgcttgga cttgtgcaaa
     2461 atctcttaca tctgctgttt gaatatatag ctcagcttct ggtaaagatt cttttattgc
     2521 ttttaggcga tcttctcggc gtgcagctat aactagttta gccccttttt ctgctaatag
     2581 cttcgttgtt gcttcaccga ttccgcttga agcgcccatg atgacaatca ctttttctga
     2641 taaatcattc attctattct cctcctgttt tgaaaaatct cttgcttata ctcactatct
     2701 cttatttcat cttactgttc aaataatccg ttcgacaggt ttgacgattt tggcagggat
     2761 tcctgcaacg atggtattgt ctgggacatc tttcgtaaca gttgcattcg ctgcaacaat
     2821 ggcattctcg ccaatcgtta cgcctggcag aatagttgct cctgctccga tccaagcatt
     2881 tttcttgatg tggattggct ttactgacaa gcctcttcgt tcttttggat cggttagatg
     2941 gttcaccgtg atgaggttga cacgaggacc aatcaatact gaatcttcta aacagatgcc
     3001 acctaagtca acgaacatca cttgacgatt gataaagaca tttttcccta tactaatatg
     3061 tttgccgaaa tctgtataaa aaggcagcag aacagtcacc gtatcatcta ctttgtcact
     3121 aatgatattt tctagccgtt tgcggatatc tgctggttca tggtacccag tattcagcgc
     3181 tgctaaaggc tcttcgtttt ccgcttggat ctgatggatc acttcaaaga gcggatccgt
     3241 tactcgaatc tctttattga cgatttgttt ttcgatgagg cttgtcattt tgctccctcc
     3301 ttttatcctt actttataac ggacggctta tgatgtctaa tacttataaa acatacttaa
     3361 ctattccttt taactatact tagtggtatg cttttttaaa aacaggaggg aaaaaatgga
     3421 tattcgtata ctgacgtatt ttttagcagt agctagagaa aaaaatatca gccgagcagc
     3481 aaaatctctg catatcacgc aacctactct ctcaaaacaa ctgaaagatt tagaagaaga
     3541 acttggtgtg acacttttca ctcgaggctc ccgagagata cagttgacgg attctggtcg
     3601 atatctttat tcaaaaggaa aagaaatctt gtcgttggta gaaaaaacgg cgaacaattt
     3661 aacaaatgac gcaatcatct ctggagaaat ctacattggc ggaggcgaaa ctaaagcaat
     3721 ctctttcatt tctgatagcc ttcattcgtt gatcaaggcg catcctgata tccagtttca
     3781 tttatatagc gggaatgcag atgatatcat agacaaactg gatagcggat tacttgattt
     3841 tggactcgta atcgaaccga caaacaaaca aaaataccag tcatttcgac tgccagccca
     3901 agatcgttgg ggacttttga tacatcggaa tcatccttta gcaaagcaaa cagtcatcca
     3961 tccgcaggaa ctgaaaactg ttcctttgat catttcccgg caaacgtcag tggacagtca
     4021 actagctgaa tggctaggtt tcagcataga tcagttatcg atcgtcggaa catataactt
     4081 attgtacaat gcttctttga tggtcgataa cgggactttt ggtgcattag ctctagatgg
     4141 gatcatcaat acttatggga cggaaaaagt attcatccca ctcgatccgc cattacaagc
     4201 ttcactgaat ctcatttgga aaaaagacca gcctctttct cctgctgctc gtaaattttt
     4261 agatgtccta acggagaaat acagttaagt agagtttttt tccttcgctt tcagcttttt
     4321 aacaacgaat aagttaagca aaagatccaa tgattaaata acataagttt cttaatttgt
     4381 tccttccttt tatttctgat aattccagta aattactata attataaaaa aagaattgac
     4441 aaaacataac tgtctatata aaattgatta cgttaacaat aattcgtttc atgtgactag
     4501 atcatactag gcatgaaaaa tctctgagga acatggtttc cctaggaggt ttttcgtgtc
     4561 tatttttgtt tggccgcaat caactggtct agcaaacgtg aaaaccaaat cttcaaaaag
     4621 ggtgcgcgca tgaaaatcaa aaagatcttt aatcaaaatg cagtattggt agatgacggt
     4681 ggacaggaaa aagtagctat tggaaaaggg attggatttg acagaaaaag aaatgatttg
     4741 atttttgatc gtgatgtcga acggatattt gtcatggaac cagaaggtca aatcaagttg
     4801 caaaatttac ttaatcagat tgatgaacgc tttttatttg cagcagaaac gatcattgat
     4861 catgcggaaa ctgttttgat ggaaaagttg aatgaacatg tattgatcgc tttgaccgac
     4921 cacattgctt tttctgcgga gaacattaat aacggtatcg tcatccgcaa caaactttta
     4981 agagaaatcg aagtgcttta cagtgatgaa ttcagtattg ctcaatgggc agtagagtat
     5041 ctgacaaaag agcttgggat tccatatact tatgacgaag caggatatat cgcaatccat
     5101 attcacagcg gacgaagcgg tcggaacaat aaccatcgca gtatccgtga agtgacgatt
     5161 gtatctgaca tcatccattt agtggaatca gaattggaaa cagatatcca ttcagaacca
     5221 ttttcactga attattcaag actggtgaat catttacgac tgcttctaca acgaacccat
     5281 gcgcaacaat atgctgtttt agacacggaa atcgtagaaa tggtcaaacg aaaataccca
     5341 gaaagctata agatttctaa aaaaatccgt gtgttgctga ccaaagaata ccagctcgcg
     5401 atcacgaaag aagaactggg ttacttggcc atccatatcg agcgtttaag aagcgcaatc
     5461 gtacaaacaa atgttaacaa tcatgaggag gaaaaaaatt gaaagcatat ctacaacgaa
     5521 tgggacgttc ccttcaactt ccagttgcgg tcttacctgc tgctgcttta ctagtaggga
     5581 tcggaaactg gtgggcatct tatagtaacg acattgtcgc tcatttttta caagctggcg
     5641 gtaacgcagt tttaggtcaa ttacctttgc tgtttgccgt tgggttagca ctagggatgt
     5701 cagtggacaa atcaggtgct gctgccttgg caggtttggt cgcatttgaa gttccagtaa
     5761 atgttttaaa aactgattca gtagctactt tattgaatat caaaccagaa tcagttgatc
     5821 catcttttgc tcagatcagt aacgtattta ttggtattat tgccggattg attgcggcag
     5881 ctctttataa tcgtttccac gaagcaaaat taccgatggc tttatctttc tttagcggca
     5941 agcgtctagt gccaatcttg gctgcctttg tcatgttgct gatcagtgca gtgctactgc
     6001 tcgtatggcc tccggtttac tctgctttag tttcttttgc taaattcatt ttgagtttag
     6061 gctggggtgg tgcaggtctt tatggcttct tcaaccgttt actgatccct acaggattgc
     6121 atcaagcaat gaactccgtg ttctggtttg acgtagcagg aatcaatgat attggtaaat
     6181 ttttggcttc tgaaggaaca aaaggcgtaa caggaatgta tcaagctgga tttttcccaa
     6241 tcatgatgtt cgggcttcca gcaggagcat atgctattta cagaaatgca cgtccagaaa
     6301 acaaagcaaa aacagcttcc ttgatgttag cagcagcttt tgcttcattc tttactggtg
     6361 taacagaacc acttgaattc tcatttatgt tcgttgcttg gccattatat gtcttgcatg
     6421 caattttcac tgggatttct ttagcagtga gtgcattctt ccattggaca gcaggatttg
     6481 cctttagtgc cggatttgtc gattacttcc tatctttgaa aaatccgatt gcgaatcagc
     6541 cattgatgtt gatcgtccaa ggattagtaa cagcagtgat ttactacttt ggctttaact
     6601 ttgctatcaa aaaattccat ctgatgactc ctggccgcga ggaagcagat ctttcagatg
     6661 acgatactgc aacgactaat tcagacaata aatatgcggc acaagcaagc aaaatctacg
     6721 ctgcattagg cggagccgac aatgtaactt cgatcgataa ctgtactaca cgccttcgtt
     6781 tgcaagtcaa agatacaggc ctgatcgatc aaaataaaat caaagccact ggcgtacctg
     6841 gtatgaagat catcgacgga aaaaatgcgc aagtcatcgt aggtacagaa gtacaattcg
     6901 ttgcagatga gatggcaaaa ctacatggtg gtgctgcaac acgacctgca caaacaaaca
     6961 ccgtggttaa aacagaaaca tttgccggtg gaacgatcga acaagatatt tacgctatcg
     7021 caaatgggaa actgattcca atcactgaag tttcagatga tgtattcgcg gaaaaaatga
     7081 tgggtgacgg ctatgctgta ttaccagaaa atggagaaat cttctctcca accgctggta
     7141 cgatcacaaa cattttccca acgaaacatg ctgtcggaat ccaaacagat gctggtatcg
     7201 aagtcttgct tcatatgggc atcaacactg ttgacttaaa aggcgaacca ttcactttat
     7261 atgtaaaaga aggtcaaaaa gtcgcaagag gtcaattgat tgctttagtt gatcttgccg
     7321 ctattcaatc tgcaggcaaa aacacggata tggtcgttgt attcacaaat ggcgacaaag
     7381 tccaaagctt agaaatcgaa cccgcacgtg atgtaaaagc aaatgataaa atcggctctg
     7441 tttccaacaa agcctgagcc atcgtcttcc ccagactttt ttatcgataa acaaacagga
     7501 gctgagacaa aaatcttgtc tcagctcctt tttccgaata aaaacggcgt tctcctgcca
     7561 actccgcggt aataagtcca gccaaacgta acatatgaag agctattttt tatttgactc
     7621 ttcttattac ttgatgcaga acagcttttt tacaacctct gataaacggt gttcaaaaac
     7681 cagtttatcg gtatgaagtc aaatttatgc aaaaatgtgg aaagccattt tcacaaaatt
     7741 tttcttcata ctcaaaacta atcggctttt tcacaacctc tttatacatt attcctgatc
     7801 caatatttct tttaatgctg cttcaagaga agttaatggt tttcccaata ctttttctaa
     7861 atcatcagag gaaatagcta actgatcctt tttaatatca tgttggatcg ctaaaaacat
     7921 gtcagctgct tcttgaggga atccggcttc ttttagattt tcgacaaacg ctgcatcact
     7981 tgcttcaata atttctaact ttttacctgt cgcacgctct aatgccttgg ctaattcttc
     8041 atagtcagtt agattgccgg ataattctaa gatttctgga gagtcacttt ctactagtac
     8101 ttttgctgca gcttctgcat attcacgttt caatgcccaa cctgtttttc catttcctgc
     8161 agcataaacg aattgaccag tttttaaagc ctgcccaatg ataggtaatt cattctctac
     8221 ataccaatta ttccgtaaaa atgtatggga aatacctgaa tccttaatca ttttttcagt
     8281 gaattggtga tctggcgcta atacactgat cgagtgatcg gcatctgcaa aacttgtata
     8341 agcgatataa gataccccag ctgcttgtgc tgccttcact acattcttat gttcggcttg
     8401 acgatttcct ggaacgccag agacaaaaag caatcgatct attcctttca acgcttcttt
     8461 cattgagtct aaatcatcat aatcaccaat acgtatatta aatccagctt ctttcagtgc
     8521 ttctcctttt tcttcactac gtactagtac atagatttca ttttgtggaa ccatcttttt
     8581 taagaattcc agcgcataac caccaaaacc tccagttgct cctgttacta aataatgcat
     8641 atttgccact ccctattctt tttatttgtt aagatttaca gctgtacatt tataaattac
     8701 acctttatca taaagagtgt ggtaagataa gtcaagcaaa aaaaatgaaa ggatgacaac
     8761 tgatgaagta ttctctgcaa tttagtgatg caatccatgt acttgcctat attgaaatgt
     8821 ttcaagatac ccgatcactt tctagtgaga tgattgccag tagtatcgaa acaaatgcag
     8881 caaatgtacg taaaatcatg agtcagctca aaaaatccgg tttgatcttt acaaaaaccg
     8941 gaaaaccgca gccttccctt gccaaaagtc cagatcagat ttctttacta gatgtctatc
     9001 gcagcatcga aggtaataca cagcttatcc aagtcgatcc aaaaacaaat ccaaattgcg
     9061 tcgttggtgc gaatatccaa gaagctttag aagaaagcta cgctcggctg cagaaaaagg
     9121 cagaagaaga aatggctaag atcacacttg atagtctgat acataaaata gccgttttgg
     9181 aaaaagaaaa aagaccagaa aacacaggcc tcgtagagag atttttataa ttctctctac
     9241 tgtgcctgca tttttctgat cggaatcgtc tacataaatt cttttattct ccgggggaca
     9301 aaagccttgt catgcccctt tttcctctct aatgctcagg tcaaagcgct gaagtcatga
     9361 cgtcttgata aacggtgttc aagtagctga ccctcggcat tagttctgaa aaatgcaaaa
     9421 tatggaaagc tatttcccat ttttcctctc taatgctcag gtcaaagcgc tactttcacg
     9481 acctctatat tatcatggtt tattgactac cggtatccag atttctccca tatatttccc
     9541 gttggtaact cccatttcga ctacagcatt tggaccccca acataggcgt aatcttctac
     9601 ttctcttaaa acatgcccaa aggccgtatt cgtcaaatca tttcttaatt cttcaggcga
     9661 ttctgctaaa gcgattacaa caagatacgt tccttttggg aatgggatga ttcgggtagt
     9721 gtttggaaca tcctgactcg tttgaacacc gacatagtac atcattttcc cgttataggg
     9781 ttcgttgact gtaaagaaat aatgattttc agcagcttgt ttcaatgcat ctattgttcc
     9841 attaccgata actttttgct caaatgcttg tttctctgca gccaaaccga caaaatccgt
     9901 attgctactc ttcaattcca caccaatacc cgtcaccgta aaatctggtt tttcttcgat
     9961 cctatacttt gccatttctc ccaccctttc tattatatta agttcatctt acataaaatt
    10021 aaagggaaat gacacgatta cgctcttttt tctagggata tttttatttt ttaactattt
    10081 ttttgctttc actgcaatcc aaatctcact gtaaacatta ctgaaatcct ctccccattt
    10141 tgtaaaagag atctcgggag cttcgactaa ctcataatct gaagaaggaa gccattctgc
    10201 atagattttc ccccaagtat cttgtaaagt ttggggaaat ggccctttat taggaaaaat
    10261 ggcccaagtg tgttcttcta tagcaagcat ttctaaatca gcgtacggat tttcttttgt
    10321 cgtagcaaag ccaatcatgt gcatcaaact gcccttttct tccaagcggg ctccatcaaa
    10381 tccataagaa acgttgatta cttgatttgg atataaatct cctaacttat gcatctcttc
    10441 cctttgagtc tccgtgatcg tctgcgctaa cttttgtatt tcttgattga ctccttcaaa
    10501 tttgacagga acttttttcg ccacacctgc cacataaaac tgttctttct tttcgatacg
    10561 aaattccatc gattttcctc ctctgacatc aataaaaaat gtcaagcgtg gaaaagattt
    10621 ttgtatgccg tatctgctga tttctgaagg cagatatcct gaccaatcac gaaaagctct
    10681 tgaaaaacct tcgactgttt catatccata tctaaacgcc gtttctgtca ctcttgatcc
    10741 atctaggaga tcgcgattag ccaaagccaa tctacggttt ttgatatagt ccattagcga
    10801 catccctgca ataaacgtaa agatgcgttt taattgatat tcagagattc ctgtattttc
    10861 ggctattatt ttataggaaa gtccttctgt caaatgttct tcgatgtaat cgatcaattg
    10921 gttcagatct ttaagcatat ttatcttcct ccttgcttgc ctttttatca tattatcaaa
    10981 gaaagtcaat tacttgatga atcaggaacc aatttagtca gatgtagaaa atgttgtatg
    11041 gatctaaaga aaattctcaa gcagcgtttc gtaacggata attggtatta tctgcctatc
    11101 tatgttttac ctttagaaaa ataaattttg gggaagacac attctaagta aacaaagcgg
    11161 taaaaatatc ttttattccc ttagttaatc cgcttactac ctactgtaca aagcaaattg
    11221 ttgttcaaac tgctgatcag aacgctgctt ggatttttct ttgcgataca tcatacctct
    11281 tttatcaatt ggaacaatta tttatttcta acaaaatcac ttcatttttg tcagataaat
    11341 aaaaaacaaa aaaagttcca tctttaaaac acgctattct tttacttttt ctcacattct
    11401 ctaagaaaag aaaaggagaa tgaatttttg acaattcgag caaaagtgat tatcaaatat
    11461 gtaaaatcag ctactttttt cttaaacgtt tctttatact aaatataaat aagtgtttca
    11521 tgaaaggaga actagatgaa taattggctt gagtattttc ctgaaaacgt tttggaacgc
    11581 ggttacagct atcatctgca cggttttgtc cgtcatttga actatacaag caagtacttg
    11641 tccgcaacag tttctggcac ggaagattat aaggtagtga tcacttggga tgaaaagacc
    11701 aacatgactt gtgattgtct atatgccata gaaggaaaaa aatgcaaaca catggctgct
    11761 gtcttattcg cttacgaaga acgtccaata aaaaaatcca actactcact ttccgaactc
    11821 tcgtcccttg tttcctccgc tagttcctct cttgtacggg agcttttaac agagattctt
    11881 atagaacatc cgcaatttat cgaacgcttc aaagttaaga tgccatttca tgcaataaat
    11941 tattcagata aattaacaac tatcatccat aaatacgatc atattatcaa gaaaaacaaa
    12001 aacaggaaaa cagcgaagtt catcatggag atgcgaaagt tcatccaaga agcagttgaa
    12061 tcgctcattc agcaaaatgc ctatcttccc gcattcgaat tgatcaatga agtgattgct
    12121 actctcgaaa cattctattg ggagcctgag gatgaacgaa cccttcttct gatagaggac
    12181 tgttattatt tatggaaaga attattagca gaagcacctc atgcagaaaa aaggcaaatg
    12241 ttctcttggt tcgtctgcca agtcgatcat actgacgcct cttattcaaa acgctactcc
    12301 ataaaaatcc tgaaagaaga ctttcgggaa aaagaatttt ctaaccaaaa gaaaaaaata
    12361 gataaaaaaa ctaaagaaac ggtaaagaaa gacgatttca atgaaaaata gcaagtaaac
    12421 gataacaacc atcctaaatc cacaagaaat acagcagacc ttgctttaaa aacacgaatc
    12481 atgcaaagga tgaagtaaag aagacttaac gcgtacccaa tattagtcat ttgactatta
    12541 ggtacgcgtt ttgttttata agtcatgaca aatttccttc gttgctgggt agattttccg
    12601 gcattgttga tagattttat gatacttttc tacattttgt aagagaggtt ccactgcatc
    12661 tgtatattgg ataaaattct gacaacaact ttcaggtgtg tcgaaccact ctaatcttta
    12721 aagataaata ttaaaataac gattaataat attaacagtc tttatttttc accaccaaaa
    12781 acctatcttt gcatatacta aatagacgta tatacacaga taggtttgtc ttgtgaaaaa
    12841 taatgattat ccttaccaaa aaatgtttgt ttttctacat gttcgtactt atcaaaaaat
    12901 atgatcctta ttgaaattac tggctattta tattccaacg acttcttgtg ttaatttttg
    12961 ttatctcata gtaaattatg tttcattttc acttaattta gttgtcacgt gttcgtcaat
    13021 tcgtaaaaac ggtaaatata taaacacact tgcggctata attaatattt gtaaaacagc
    13081 tgctcgccag tctccggctg ttgctaaaaa tcccgatata actggtggcg tagtccaagg
    13141 aatttgcact actacatgat taattaatcc cacagcagtt gcaaaatatg ctactgtcaa
    13201 agaaaaaatc ggtgcaagca caaagggaat aatcagactg atattaaata caataggcaa
    13261 tccaaaaata ataggttcgc ttatattgaa tatagcggga gttaccgata atttagcaaa
    13321 gtccttataa tcttttctac gactgaagat aaatattgca atcaataaag caatcgtatt
    13381 tcccgatcca cccattaccg caaaggtatc tttaaaggcc atattgataa tatgtggaat
    13441 ttgctctttg tttgcataag ctgccatatt ttcttgcata ttctgaatca aaaacggatc
    13501 taacaatgaa cctgatatta ctgcttgatg gataccaaat ccaaaaaata gattagcaat
    13561 agatgtaatt aacaaaaatc ctggtaaacc agttgtaatt ttacttaatg gctctgtaac
    13621 tatcgtggta attagcttat ttatatccat attaaacaat tgaatgatta taaatgacaa
    13681 taaagcaaac gaggtaatcg taataatgat tgggattaat acattaaaag actttaaaac
    13741 agcgggagga atatttccgg atagctgaat ctgtagtttc ttattattag aaagtttaat
    13801 aaataattct gtagccgcta atccaacaaa tatacctaca aacattcctg aagctcctgt
    13861 ataggatata ggtatcacac ccgtcacttc aacagcttta tccgcatttt caggagtaaa
    13921 catcatctgc ataggtgtta atacaacaaa tgtcgaaata gcaactaata ccgctgcaat
    13981 atgatttgta taatttctgc ttgcacacat atgataagat actgctgcta caactaataa
    14041 tgtagttatg cttaatgtac cattggcaat tgaagtacca atttgttgta acgtggtcaa
    14101 tgtactagta tcgacaattt cccccatgaa tcctgttggt tctaagatca ctgaattaat
    14161 aagtgtcaca aatcctgcta acatcataaa tggcataaat gtagcaaacg catctcgcaa
    14221 agttcttaaa tggatctgat tccccaactt agtcgcaact ataccaaatt tttcaaatac
    14281 aatgtccttc ctgctacttt tgcccatatt ttttctcctt tatgcttatt gtaatcgatt
    14341 actttttcca cccaaataat acatcactta tcaaaaaaag taaatacttt taattaaata
    14401 tatatatctt ttttagtata aaaaaataag ctctgaaaaa ataattttcc cgagcttatt
    14461 tttcaacaaa aacagcttca aaaacaaagt cttgatataa atgtctcgat accgaatact
    14521 cgaaaggttt accagacgat agagcaacaa ctttgtctat ctcaatcata aattcacctg
    14581 gatctatttt taatattttt gcgtcaaatt cattcgcagt atctcctttg atacgtatgt
    14641 gcgaagtctt tatttccaga cccagttcat ttgtgatata agcatataca gatttcttta
    14701 aatgttttgg ttccaagcct ggtatgactg ctaatggcat gaatgtttgc tcaatcgagt
    14761 atggttgaca atcaataaaa cggcttcgta caatattata tacatagtct tttacactga
    14821 tacttaattt atcagctact tttttcgatg gaagttcaat tgagaaagta tggatttctg
    14881 aatctacgtg ttcttcacca accacgctaa acaaaccttc gtttggacca agaactcgtg
    14941 agttatcaat tggtgttctg acgacttcag tcccataccc acttctactt cgcaaaactc
    15001 cttcggaaac aagtaagtct acgcctttct ttactgttaa cttgcttgta tgataaataa
    15061 ctgccaattc atctcctgta ggaagtttct caccaatttt atactctcca tttacaattt
    15121 ttttctttat atctaagtaa atttccatat acttcgcaat ttttttcaat ggtgaccctc
    15181 caatcaaaaa catatcttca tctatacttt aacataaata aaccgataaa atccacatca
    15241 aaattataca cacaataaaa aagatatata tatttaaaca aaactattta ctttaagttg
    15301 taagcggtgt tatcattaag tggaaacagg aggtaaaaaa attatgacaa ctatgaaaaa
    15361 gctaactttt ccagatgatt tttggtgggg ttccgcttgg tcagctgagc aagcagaagg
    15421 aaaaggagaa acaggaaaag cagagactat ctgggatcgc tggtttaaag aacaacctta
    15481 ccggttttat caacgaatcg attcctctat tacaaccgat cacatccatc aatataaaga
    15541 agatatagaa ctaatgaaaa aaactgggca taactccttt cgcgtttcta tttcttgggc
    15601 acgaatgttt ccaaatggcg gtaccggaaa agtaaatccc aaagccattt ctttctatcg
    15661 aaaattattt tccgaaatga ataataacgg aatcaaagta tttgccaact tatatcattt
    15721 tgacatgcct gctactcttc aagataaagg aggatgggaa tcacgtgaag tagttgatgc
    15781 ttatgttcat tacgctgaaa cttgttttaa agaatttggt gacttagttt gtcattggtt
    15841 taccttcaat gaaccacttg ggcctatttt aggtacttat cttgaagatt ttcattatcc
    15901 aaacctcatt gactttaaac gcggagcgca agcagcattc ttcactatac ttgcacacgc
    15961 aaaagcaata caagtattta aagaactagc tcttcctagt aaaataggcg ttattctaaa
    16021 tttgagtcct acttttcctc gtagtcaaaa tcctgctgat gtgaaagcag cagaaatcgc
    16081 tgatttattt tatacgcgga gctttttaga tcctatggtt aaagggaatt tttctaaaaa
    16141 gcttatcctg ttattacaag aatataatca gcttcctgaa gattatacga aagaagatct
    16201 tgtatgtatc tacgaaaata ccgctcaaat tcttggacta aactattacg agccacgtcg
    16261 cgtcaaatct cgttcttcta tggtggatac atctagtcca tttttaccag agtggttttt
    16321 cgaaccacat atcatgcctg gacgacgaat gaacgaatac cggggatggg aaatttacga
    16381 aaaaggaatt tatgatttgt gcatggatat caagaaccat tatggaaaca tcgaagcatt
    16441 tatctcggag aacggaatgg gcgttgcgga tgaagaacgt ttcatggatg atacagggca
    16501 aataattgat gattatcgga ttgagtatat caaagatcat cttgcttact tatggaaggc
    16561 aataaatgaa ggatgtaaca ttaaggggta tcatctttgg acatttattg attgctggtc
    16621 atggatcaac gcctataaaa accgatatgg gctggtttca ctcgacctta ctactcaaaa
    16681 aagaacaatt aaaaaaagtg gcgaatttta caagcaatta agtgatacaa atggatttag
    16741 atacgatgta gacttgttag ttgaataata ttctaaaatt ccacttgaag tgaacacatt
    16801 agaaaacaag aataatgaga aataccaata acaaatactt tttcttcata cttcatttat
    16861 acttgtcagt ataagagact gtgacaaatg tagtgatcct cagaagttag gtctggaaat
    16921 ctaacttgag aagatttttt taaactacgt ccagtctttt ttatttgttt aaaattgaag
    16981 ttgacaagtc ttcattaaaa atttagaata ttcacaagtt tgacgacaat tatgaaaatt
    17041 tatgtcgtgc taaaggagtt ttgttattgg atgaaaagtt agatataaaa actgcgatca
    17101 aagacacgtt acctaccgtt tttggttata tcggtattgg acttgcattt ggtatcgttg
    17161 ggaaagctgc cgggtttcat ccgctggtcg tcacgttgat gtccctgctg atctatgctg
    17221 gttctgccca atttatcacg gtcagcatgc ttgctagcca tagtccactg ctctccatcg
    17281 ttctctcgac ctttctagtc aattcccgaa tgattctgat gagtatgacg attgctcctt
    17341 atttcaagaa aaatcggctg cttcaaaatc tgctgattgg cacattgctg acagatgaaa
    17401 gtttcgcact gggaatgaat aaattgaact atactggaca aaagttaaac tttcgttgga
    17461 tgaatacagc taattggatt tcttatttga cgtgggtggg ttcttctctt gttggcgcac
    17521 ttttagggaa ttttattact gatccgaaaa agtttggttt ggatttcgcc atcgtcgcta
    17581 tgtttatcgg gttactttat cttcaagtca tttctgatag aaacacctct aaacgattac
    17641 agttgatcct gattggtctc actttgattc tggtatatgt cggactgatt tttatcccaa
    17701 gtaacttagt gattttagtt gtaacactga ttgggtgcgg attgggggtg tggatcaaac
    17761 atgccttctt ttgagtttac attgctgact attttaggtt gtactttagc aacttggctt
    17821 tctaggatcc taccatttgt cttactgaaa aaattcgatt tgccgcaacc gttgcttgag
    17881 tatttaagtt ttgtcccgat cgtcattatg tctgctttat ggttcagcag tctatttacc
    17941 caaaatatcg gacatctgcc gcagatcaat ctcgaaaatg ctttggcttc agttcctaca
    18001 cttttagctg ctattctttc aaaaagctta ttggtgatcg tcttggtagg aattctttcc
    18061 ctttcgttga ttcgcttatt ataaataaaa aaacaagagc ttatggcaaa ttactgtcat
    18121 gcgctcttgt ttttatcaaa gtcaaataag aaaaaaatcg gcgtattctt gcttatccgt
    18181 tgaacggacc actaacaacg acttttccta atatatgtcc tgtttctacg tctttggttg
    18241 cttgtttcaa gttcttcgca ttcatcccat ctgaatagac cttgctcaat gtactgcgga
    18301 taatcccttc atttgctaaa gcagcaatgt gcgctaaagc ttctccttgc gtttcgattt
    18361 catagttata gtctgtttta gcgaacatat actcccagtc gaagctgata gattggtttt
    18421 tccaaacacc tatatctaac ggctctttga tccctacgat cgttcccaca tgtccaaatg
    18481 gtcggacaag gtgtttgatt tgatcaaagt agcgggtaat atcgaataat acagcgacat
    18541 actctacttg atcgattcct aattcacgaa aacttttact taagtcgtga tgataatcga
    18601 ttggatagtc tacacctgtt tttttcagcc attcaaaatt cttcggactt gcagtcgcgt
    18661 agacttctag gccagcccaa tgcgctagtt gattcaaaat cgaaccgacg cccccagctc
    18721 cgttgatcac taagattttt ttcccgcggt tggcattttc ttctggagtc agaccaaatt
    18781 tttcaaataa caattcataa gcggtcaatg aggtcaacgg caaggctgcg gcttcttcat
    18841 ctgaaagatt ttttggtgca agagcgacaa tacgttcgtc aaccagttgg tattcttgat
    18901 tgcttcctgc tcttgtcgtt gtacctgcgt aaaaaacgcg atcgccgaca gaaaattttt
    18961 gcacttcatc acctacagca acaactttcc cgactccatc aaaccctaag atacgtaaag
    19021 catttcttat cccgttatct tggcgaagtt tcgtatcgac aggattaaca gatactgcgt
    19081 tgacttttac cagaagatcc cgtgaactgg gtacaggtat ctgttctttt tcatcaataa
    19141 agctttttgg atcatcgatc gacaaccctt cataaaaacc gactaccttc attaattcct
    19201 tttctgacat acgaattcct ccttagcttc atcaagttgg agcatccata cagttttcat
    19261 ttcaatatat atagtatgct atttgttcca cttttattca tcataattat tctcttactt
    19321 aaagtaaact atttgcactt ttttggttaa aaaagaggtt gcgacaagac ttttgtcaca
    19381 acctctttta aacggtgttc aatcaactga ccctcggtat tcgctcaaaa aacgacaaaa
    19441 tatgaggagc tatttttgtt tggctgttct tattacttga tgcaggacag ctttttcacg
    19501 acctctaatt cacggtgttc aagcaagaaa tcattgttca ctgaaaagcg ttggatgttc
    19561 tttttcaaag gtgtctaaga tctctcgtac ttcatcagta gatttcgtat tcatcaactg
    19621 aactcgcaag tcatttgctc ctggaaatcc tttgatatag attttaaaga aacgatgtag
    19681 tcctgtgatg gaacgcggga tgatttctga ataatgatct tgcagatcta gttgcagacg
    19741 aagtagtccg atcagttctt tttctgtatg ttccctcggt tctttttcaa atgcataagg
    19801 attcttgaaa atcccccggc caatcatgat gccatctacg ccatattttt cggccaattc
    19861 ttctcctgtc tggcgatctg ggatatctcc gttgatcgtg atcagtgttt gcggagcgat
    19921 ttggtcacgt aaagcaatga ttttagggat gacttcccaa tgtgcatcta ctttactcat
    19981 ctctttacgc gtacgcaaat ggacagaaag attggctata tcttgatgaa gtaggtgact
    20041 gatccattct tccatctcag acatttccgt atagccgata cgcgtcttga cgctgacagg
    20101 caaaccgcca gctttagccg cttcaatcaa ttgtgccgct acttctgggc gaaggatcaa
    20161 gccactgcct tttccacgtt ctgctacatt agggactggg cagcccatgt tgatatcgat
    20221 tccttggaag cccatttctg ccatagcaat gctcatttct ctaaaaaatt ctggcttgtc
    20281 tccccaaata tgggcaacca tcggctgttc atcttctgta aaggtcaatc ttcctcgtac
    20341 actgtctttt ccatctggat gacagaaact gtctgagttt gtaaactcag taaagaagac
    20401 atccggtgct gctgcatgct ttacgacatg gcgaaataca acatctgtta catcttccat
    20461 tggtgctaaa acaaaaaaag gctttggcag ctctttccaa aaattagtag tcatttattt
    20521 gttactctcc attctatcaa gcagtgaagt cgcaatcttt ctcaaaaaca ttgtcattat
    20581 actaggaaac aaaaaaagaa gcaaagtgaa cagcttaaaa gatgtttctg attgagcaaa
    20641 aaaagactgt gacaaaaatc ttatcacaat tccttttccg gaataaatgg tgttcaaaag
    20701 cttgcatctg cggtaataag tccaaccaaa cgaaaaatag gaggagctat ttttgtttgg
    20761 ctgttcttat tacttgatgc agaacagctt tttcacaacc tctccactaa acggtgttca
    20821 atcagctgac cctcggtatt agctcaacaa acgacaaaat atgaggaact attttcccgt
    20881 ttgtttctct aatactcagg tcaaaacgct gatttcacaa cctcttttct aacttctcaa
    20941 tcctcttcct ttattgatca aataccaagt aacggcgtac aaaacaacga tgaatagcac
    21001 aagtacgatc atcgatgttg ctactggcac atcgattgtt ccaaggaatc cgtaacggaa
    21061 accgaagatc atataaacga tcggattgac tttcgaaaca gcttgccaaa atggcggcaa
    21121 catagagatg gcatagaaaa cgccacctag ataagttaac ggctgcaaga caaatgtagg
    21181 cacgatcgat acatcgtcat aagactgagc aaaaatcccg ttgagcagtc ctgctaacga
    21241 aaacaagatt gccgtcatca ataatgtaat gatgaccatg aaccatgaat acacatgaag
    21301 tggtacaaag aacaaggaaa tgatcgttac gagagagccg acgagaatac tgcgacccac
    21361 tccgccaatc acaaagcccc aaatgatcac atgcgtagga acaggcgcta ccaagatttc
    21421 ttcgatgttt ttctggaatt tttgagagaa gaaggacgaa gaaacgttgg catacgaact
    21481 agtgatagca gacatcataa tcaaaccagg cacgatgaat tccatatatg aaaaaccacc
    21541 catgtcaccg atccgaccac cgatcatttt tccgaaaata acaaagtaca aggatgtcgt
    21601 gatcacaggc ggaaccagtg tttggaccca gatacgcaaa tagcggttcg tttccttgat
    21661 agctaaactt tttaatgcag taaaataaag attaaacatt tttctcctcc gtatgattat
    21721 tttcttctgt gattttcaaa aacagttctt ctaaacgatt cgacttattc ctcatagaaa
    21781 gaactttgat tccttgtctt gtcagctgat cgaagattcc gttcacccct tgatttcgtt
    21841 caacttcgac agcgattgtc tgttcatctt caaatgtaca agcgtatcct tcaatcacag
    21901 gtttggtctc atatggcgct aaatcaaaga taaatgtttc aaattgcagt ttggatagaa
    21961 gctctttcat actagtattc tcgatcagtt cacctgattg gatgatcccg atgttgcgac
    22021 aaagcatttc agcttcttct aaataatgcg tcgtcaaaat gatcgttgta ccattttcgt
    22081 tcaattctcg gagaaactcc cacatctcgc gtctcaattc aatatcgact cctgctgttg
    22141 gttcatccaa gatcagcaaa cgtggttcat gcatcaatgc cctagcaatc atcagtcttc
    22201 gcttcattcc cccggaaagc atacgtgcac ggacatttct cttttcccaa agattggact
    22261 gcttcaaata tttttcgctg cgtttcatcg cttctttgcg agagacaccg tagtagcctg
    22321 cttgattcac gacgatttgc tggaccgttt caaagggatt gaaattgaac tcttgcggga
    22381 ctaatccaat ctgttgcttt gctcggacta gatccgtatc cagatcataa ccgaagacat
    22441 tcacttttcc agatgtttta ttgactaaag aagtaatgat tccgatcgtt gtagatttcc
    22501 cagctccatt tggtcctaat agcgcataga aatctccttc tttcacggaa agatcgattc
    22561 ctcgcaatgc ttcgacccca cttccataaa cctttttcaa attatttatt tcaagcgcat
    22621 ttgccattta tttcctatct ccttatagac atgattcttt attgtatcat ggaatacagc
    22681 agatgaaaaa acaacttggt tccagcgaaa aatcaggttg ctgggctcag gaaaatctcc
    22741 taatgaaaga catcaatcgg acgaagtcag acaacctttt cagaaaaaac aaatcgaaga
    22801 taaaggatta ctattcatat gtgcaacaaa aggagcgtga gacaaaagta aagaacactt
    22861 ttgtctcacg ctctaaacac gtataaacgg cggaagcaaa agccaagatt tttgatccaa
    22921 taaatacttt cgatcacttc gcctatccca atacatgcca ctgccgcttt tcatcaaata
    22981 acgcggcttc cgtttttgcg gtttctacta aagtctctgg gtaatccaaa actttcagca
    23041 aggcaatcgc atttcgggtc cttgaaggtc cttcttttaa tttgaaatca aaactgattc
    23101 cttttccttc tgttacttgt tcttcaaagt ggacattctc acaataattc tttaaaatct
    23161 ctgtcaattc gatatcatgt gtggcgacaa atgctaaaga tggatactcc gcaagccacc
    23221 gaacgactga agcggaagaa gcaatccgtt cgatcgtatt tgtccctttt aaaatctcat
    23281 ctacgaagca gtagcagcgt tctttggaag ccacttggtc caacaaacgt ttgatcgatt
    23341 tgatttctga aacaaaataa ctgtcgcctt caaataaatc atcttcgatt gccatggaag
    23401 tcaacacatg tccaggttgc agggtaaact gttctgcaat cgctgtttgg atcgtctgtg
    23461 ctagaataca gctgatggct atacttttga catacgtaga ttttcctgat gcattggaac
    23521 ccgtgactaa tgtattcttc tgccagttga ccggattcac taccgcttct tttaacaatg
    23581 gatgatagat atctttcgct gtcacaccgc cctcagtaaa ctctggtcgg caagtgatgg
    23641 gcatataagt acggtaattc aatattgcag cggctacttc taattctccc agcaattccc
    23701 acagcttgat ggcttcagtt gaatgctttt ctaatttagt gagaacgaaa tgataggaga
    23761 taaatggcag catgaacatg atgttgacat attcaaaaat caactctgct tcactcccac
    23821 tcttcatacg aaatgaaaaa cctaatttag ggatgtctct taatggttta gtttgctgtt
    23881 tcagctcttc ttgaagtggt gtcggcagtt ttgctagctg attggcggct gaaatcgttg
    23941 aaaccaaata actcatgcta ttcagctctg tttctagtgt ttgtttcttc actagataat
    24001 agaccgtatt gaatactaac ccaccgattg ccaaatagat tcctagtact tgtcctaaaa
    24061 gaagcaaacc caaacccacc agcggaagca agccaagaaa aataaataaa cctacgcgtc
    24121 caatcttttt actttttcca tccgctagat attgttttgc aaaattatga tctttttttc
    24181 ctaaacgagc gaattgatat tggatttttt ctctcagttg aggattttcc tgataaaaag
    24241 caatcagttt ttctaactgc tgatcttcac caaaatcaaa gctgcgtaaa cgttgataca
    24301 acgcttctga acctacacta gaataggtac tgttgattcc ttcgaatact tcgaacatat
    24361 ccaggtcata ccaagtaaga tcatcgattt cgctatccca tgaacggaac ttcttctccg
    24421 tgagccatgc ttccttcaag ctttcttctt tatcaaatct cggttgataa ggaatttttt
    24481 cccatttgct acgaactgtt tcctttaatt tcaaacgtga aaaaatgtct aaagcaacta
    24541 ataaaataac agctgcaata ataattaaaa cgataaattc tctttgaccc atatccctct
    24601 ccgttccttt atttgttagc tttagtatag cactgttgcg ctatttgaaa agagggtaaa
    24661 aaaacacctt tcaaaataaa aggtgttttt ttcttgtata ttacatattt atctctcatt
    24721 tatcaaaatt gacaaataat catagggagg aattgttgtt aattcttgtt tattctccag
    24781 tgcattgaca actgtcccaa gggaaaggat agcctcaggt actatttttt cttgttctgt
    24841 tggattagct aagcgaagaa caaatccttg ttcatgaaaa taagaaggat aaatcgatga
    24901 tacaattaag tcttcaggta attcaatcaa tgaaaattta cgaggaacat caagttcatt
    24961 ttgtaatggc caaatcttgt tatctaacct gtgaataaat acattcagtt tctgcttttg
    25021 ataagaaata gattgttcta accgtttttc tgcctgtttt gccactttga actcattaaa
    25081 tcttccttct tctacattaa atccaaaagt gactttatac tcacctatct cttgagctag
    25141 cggtgtttcc atcatgacat gcccttcatt tgtcgtatct cctgaagctc gcccaggtcg
    25201 ccaagcaaga ttaggttttc caagttcccc tgttgttgaa aataaggtta tgattaattg
    25261 attttctata cgttcatact cttttaaacc atctgcataa aacgtaaaat aatgatcatt
    25321 ttctgccaca gagacactct tatcgaatat ttctaaatta acaggtttct ctaccatagt
    25381 ttctgaccaa ttcggaggaa tgacatcagg ggtattttca ataaatccgt tttgaatttg
    25441 tgcaattgat ttttcagatt tatctaaaac attaattgct aagcgcatac gatgcgagta
    25501 aacagtatta tgaattttga gtgtgccttc aacgatttga ctttctccca gactgaacga
    25561 tatttcataa gatatttctt ccatttcggg tttttcagat aaacgatctt ttaaatcttt
    25621 aggaagttga gcttttccgt aaacaactaa tgtttcttgt aaagaatctt tatatacttt
    25681 agctgtttct aattttagag atagttctgt atctccttct aatggtgaat aatcatacgt
    25741 atcgccatca tttgctgaat cgatcaaatg aacgaaatca agatattgtc tatctccaac
    25801 tatcaaattc aattgcccat ctttaaaaga aagtttatat ttattgttac ttatctcagt
    25861 tttgtctatc tcgattgctg tttctaattt ttcattattt ttctcaaatt caacaacagt
    25921 gtatcctagt gctggcacat ttacatttat ttttatgtca agttcataat aagctggttc
    25981 atcaaaatat tcgaaacctg tagcaatcat ccgataagca tttttccgtt ctggaaaata
    26041 aacttctttt tcaatgatat tatagggact acctttgaag tgtattttct ttgaatcagc
    26101 tactacatgc acgattttcg atccactata attctcagtc gttgtgttga aaacaaggac
    26161 ttcattcgat tttagcttta atgcatcagc catccgtttt acaataaggt tttcgattcc
    26221 gtcagctatc tcattagctt ctttgattcg atggaaaata tcttccgcaa cattatctga
    26281 aacacagcca ccaatactat catgagcctg gttttgcaaa atctttttcc ataaccgacg
    26341 aagtaggcct ttactgatat ggataccatt tctttctgca atcagcatga gcggctcaac
    26401 tcgttttaga attttttgtt ccaatttaaa attttctatt ttcatttttg ttcttactga
    26461 gccaatagaa cgatgaacac gagagtaaac cggctgacga agttcacctt tatattcagg
    26521 taattctttt ttgtttctga taacttctac ataagaagga taatctgaaa taatattgtg
    26581 gaatttactg tatttattca acttctctac gacttgatca aaatttaaaa tcattgactt
    26641 ttgatctaca cccgaaggta ctaaaatatc tttgtttttt ccttgcatat gaagcatttc
    26701 aactgcttca tccaactttt cttttgcaaa ttcaggaata tctttaattg cgtctaaggt
    26761 catcattcca gtcatataac caaatggaaa attcatggca taaacaaatt tatctcccaa
    26821 tcctttccag ataaaataag gagatggaac catcgtttca aagtttaatc ctcgccaaca
    26881 catgaaatta tcgatttcta cttgatgcaa aagtgtaggc aaattcgcat tgaacccaaa
    26941 cgtgtcaggc aaatacccta ccatcattgg atctccatat ttaaggatcg tatcactgat
    27001 tccaatcatt aagtttctta aaattgattc agcatccact aataaggcat ctgtttgtgc
    27061 ataccacggt cccacgaata gttgtcctgt tttcatcagt cgttggataa ccttaagctt
    27121 ttgcggattg atctccaaat actcatcgac gattgaagtt tgtccatcta aacaaaagtt
    27181 tactttaggg ttcctttcca attcatctag aacttcagta aagacctgat cagacaatac
    27241 gatggcatct tcatttgtaa aataccattc tctgtcccag tgcgtgtgtt gaattatatg
    27301 tgcatttacc atatcagatt ctcctacttt ccttcttcta ctgaatttac ttgttgttct
    27361 tttttatact tgatggattt tagtgcattg accattaacg ctgtaaccac tcccccaata
    27421 acaattgata ctaaatataa aggtatattc gtaacaaaag gaattgccat aaaggttgaa
    27481 agcattgttt cttgagtaac accaaaaaat ccatttagac cacatgctac tgcagtgccc
    27541 accacgatgc ttggtattac tcgtaaagga tctttcacgg caaacgggat cgtcccttca
    27601 gtaatcccca caaggcccat aatcatagct gtctttgcat tatctcgttc cgattcatca
    27661 taaagttttt tgtttaaaat tgttgctaaa aacattccaa atggcggaat agcaattcct
    27721 aataacatgg ctgtactagg tccaaagctt ccgcttgaaa aagaagcaac cccaaaagca
    27781 tatgcgattt tattgatagg tcctcccata tcaaacgcca tcattcctcc taaaatagcc
    27841 gacatcaaag cagcatttgc tcctgacatg ccttctagcc aattagtcag tcctgttgtc
    27901 attgcttgaa taggcgtagc tagtacatac cataatatga ttccaacaac taatgtagta
    27961 acaaaaggaa tcagcataat tgattttaga ctaataactt gcatcggtac ttttactttt
    28021 ttcaatgcat tagctaaata gccacaagct aatccagcaa ccattcctcc aataaatccc
    28081 ataccaagat tttgacacat catcccagtg ataaaagctg gtgctagacc gggacggtca
    28141 gcaattgaat atgcgatata ggctgaaatc atcggtacga taaactgtaa tcccgtctga
    28201 ccaatcattc gcaggactcc ccagaatgta tccgtctggt cccaaacacc tgtgccacca
    28261 aaaatgactg caatagaagt tagtaaccct gctgctacga caagcgggat catataggaa
    28321 accccacttt ggaaatgatt tagaatttct cttccttgtt tcttgaattc cattagtaac
    28381 tcccccacga tatatttttt atttgttaat taatgcaatt gcttcatcaa tcgtctcttc
    28441 accatctttt actacctctg aggtccctac tgttaaaatc ggtttaccgt caaagcgacc
    28501 tcgttctgaa acatttgtat ctactgcaaa tatgacaaca tctgcctcgg aaacatccga
    28561 aattgaaagt ttgttttcta tccccatagc cccctgtgtt tctactttaa tatctaaccc
    28621 tttcttcttc gcaaactttt ctaaattagc ttttgccata taagtatggg caatccctgc
    28681 gatacatgct gttactgcta ctatttttgt catatgaaat tcctcctttt aaaatgatag
    28741 agcttttacc aattcttttt tatttctcga atcttttatt cttttaacaa actcgtcatc
    28801 catcaatctt ccagataatt ctgctaaaat acgtaaatga ttagtacctt tatcagtgtc
    28861 ttttattgct aacaaaaaga cgagatcaac tggttgatca tctaatgcgc cccattcaac
    28921 agaccgcttt aacttggcta cagccactgt tgattcaatt actgctaggc ttttgccatg
    28981 aggaatggcg atatttttcc caattcctgt ggtcatatgg ttttctctgt caaatacgtc
    29041 tttttgaaat tgtttgtggt cagacacaaa cccattagtc tctaacaatt tcgataattt
    29101 ttcgactaca ctttttttat cagatgcatt taaatctatc actatattat ttatattgat
    29161 tttttcactg atcatttata agccttcttt caatatataa actaattttt caaa
//
LOCUS       DAZUMK010000020        29110 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000020 DAZUMK010000000
VERSION     DAZUMK010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.020"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1030)
                     /locus_tag="ACG808_000919"
     CDS             complement(<1..1030)
                     /locus_tag="ACG808_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5046566.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLRDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1112..1801)
                     /locus_tag="ACG808_000920"
     CDS             complement(1112..1801)
                     /locus_tag="ACG808_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFS5046567.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="ACG808_000921"
     CDS             complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="ACG808_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFS5046568.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2328..2831)
                     /gene="rplJ"
                     /locus_tag="ACG808_000922"
     CDS             complement(2328..2831)
                     /gene="rplJ"
                     /locus_tag="ACG808_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFS5046569.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3035..3162)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="ACG808_000923"
     CDS             complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="ACG808_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFS5046570.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="ACG808_000924"
     CDS             complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="ACG808_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFS5046571.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4799..5467
                     /gene="sdaAB"
                     /locus_tag="ACG808_000925"
     CDS             4799..5467
                     /gene="sdaAB"
                     /locus_tag="ACG808_000925"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFS5046572.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5480..6352
                     /gene="sdaAA"
                     /locus_tag="ACG808_000926"
     CDS             5480..6352
                     /gene="sdaAA"
                     /locus_tag="ACG808_000926"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFS5046573.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6443..7543)
                     /locus_tag="ACG808_000927"
                     /pseudo
     CDS             complement(6443..7543)
                     /locus_tag="ACG808_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(7635..8522)
                     /locus_tag="ACG808_000928"
     CDS             complement(7635..8522)
                     /locus_tag="ACG808_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS5046574.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="ACG808_000929"
     CDS             complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="ACG808_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFS5046575.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9245..9415)
                     /gene="secE"
                     /locus_tag="ACG808_000930"
     CDS             complement(9245..9415)
                     /gene="secE"
                     /locus_tag="ACG808_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFS5046576.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="ACG808_000931"
     CDS             complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="ACG808_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFS5046577.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9753..10397
                     /gene="cbpA"
                     /locus_tag="ACG808_000932"
     CDS             9753..10397
                     /gene="cbpA"
                     /locus_tag="ACG808_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFS5046578.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10453..10956)
                     /locus_tag="ACG808_000933"
     CDS             complement(10453..10956)
                     /locus_tag="ACG808_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFS5046579.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10969..11015)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11553..12518
                     /locus_tag="ACG808_000934"
     CDS             11553..12518
                     /locus_tag="ACG808_000934"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS5046580.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12617..13789)
                     /locus_tag="ACG808_000935"
     CDS             complement(12617..13789)
                     /locus_tag="ACG808_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5046581.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13776..14921)
                     /locus_tag="ACG808_000936"
     CDS             complement(13776..14921)
                     /locus_tag="ACG808_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5046582.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14935..15867)
                     /locus_tag="ACG808_000937"
     CDS             complement(14935..15867)
                     /locus_tag="ACG808_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5046583.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15984..17063
                     /locus_tag="ACG808_000938"
     CDS             15984..17063
                     /locus_tag="ACG808_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFS5046584.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17060..17689
                     /locus_tag="ACG808_000939"
     CDS             17060..17689
                     /locus_tag="ACG808_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS5046585.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17799..18026)
                     /locus_tag="ACG808_000940"
     CDS             complement(17799..18026)
                     /locus_tag="ACG808_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS5046586.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18129..20717)
                     /locus_tag="ACG808_000941"
     CDS             complement(18129..20717)
                     /locus_tag="ACG808_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFS5046587.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20737..21396)
                     /locus_tag="ACG808_000942"
     CDS             complement(20737..21396)
                     /locus_tag="ACG808_000942"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS5046588.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="ACG808_000943"
     CDS             complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="ACG808_000943"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFS5046589.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22169..23035)
                     /locus_tag="ACG808_000944"
     CDS             complement(22169..23035)
                     /locus_tag="ACG808_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFS5046590.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23144..23785
                     /locus_tag="ACG808_000945"
     CDS             23144..23785
                     /locus_tag="ACG808_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFS5046591.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="ACG808_000946"
     CDS             complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="ACG808_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFS5046592.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPLKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25226..26125)
                     /locus_tag="ACG808_000947"
     CDS             complement(25226..26125)
                     /locus_tag="ACG808_000947"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFS5046593.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26127..26924)
                     /locus_tag="ACG808_000948"
     CDS             complement(26127..26924)
                     /locus_tag="ACG808_000948"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFS5046594.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26902..27606)
                     /locus_tag="ACG808_000949"
     CDS             complement(26902..27606)
                     /locus_tag="ACG808_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFS5046595.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27737..28312
                     /locus_tag="ACG808_000950"
     CDS             27737..28312
                     /locus_tag="ACG808_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFS5046596.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28430..29107
                     /locus_tag="ACG808_000951"
     CDS             28430..29107
                     /locus_tag="ACG808_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFS5046597.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8566 a         6167 c         5233 g         9144 t
ORIGIN      
        1 atacagtatt atttcggttg cttgctgctt cacctagccc ttgtactcgt gtttgattgg
       61 cgacttgacc aaattgattc ccgacgtcta ataaaataat agctaaaagc aaaatggcca
      121 aatggcttcc gccaaccaat aaagcaataa agcttattgt ttgcataaca agtcccatat
      181 acacgatttt tctttcagaa taagaatctg ccagtcgtcc cacgataggt gcagcaactg
      241 ctccagacaa accaaaaatc gctaatatcc ctgcttcgaa tgttccccaa ttgtaggcgg
      301 gactgctgat ataaaaaatc aacgtagacc aaaagatcga aaatgttcca aacatgaaaa
      361 atccactgac tgccgcttct cttaatagac gttgagaagc aactaaatgc ggcaaacttt
      421 taagtgaatc aaggtatgaa acagctgtac tactttgctg gacagaacgc ggcattttcc
      481 agtaaagcag aagtgtaaga attccgactg cacataaagc aaataaatag atcatccgcc
      541 aagaaaaaag actggcaaca ataccagata ccgtccgaga aagcaaaatc cccacgagta
      601 aaccgctaag caatgtaccc atgaccttcc ctcttgcttt tggtcccgct agtaccgcac
      661 cgtaaggaat gatgatttgc ggcacgatcg aaagtaatcc tattaaaaaa gaagcgcatg
      721 caaataacgt aaacgaaggc gcaaaaaaag cggccaacag agagacggca gataacgcta
      781 ccattcgaat gatcaatttt ggacgatcca ccacgtccct taaaggaact aaaaacagta
      841 accctaacgc atagcctaat tgggtcaaca tcgtcaatat cccgactgca cttgtgctaa
      901 cagataaact agctgctact tttgttccaa tcggctggat gtaatacata ttggcaacaa
      961 caacgccaca agtgattgat aatagtaaaa taagcgaagt tgttaaaggt gttttttcat
     1021 tactcttcat tgatttcccc catttcctat cttagtttac aacttatgga aaagattgga
     1081 aatagaaaaa tacaactatt cgccacactt attatttgtg cttttgtttt ccttgttttt
     1141 tcttgcgatc tttataaatg aagacaacta accctactag cacagtaaca cttgcaacca
     1201 acgtgactgc agttttgtta tatccgtgaa acagagcttc tccaccacac gcatacaaca
     1261 cagacgaagg gatgactcca atcacgatcg acaatatgat ttgctgtaga ggcaatttca
     1321 tcgcatctgc agcaaaattg accatagaag atggaataac cggtatcata tagcctaaca
     1381 ttacacctat ttttggatgt ttcatttgtc ggattgcttg cacccaatga ttcgtttcgg
     1441 ttttcttatc taaaaatttt aagcgatgca taaggaaaat tgcagtgatg ttaccaagag
     1501 cattacccaa aatgttgatg atcgcacctg tcagtggtcc aaagctcaaa ccagcaatga
     1561 ctccaactac tgaagtagga atacctggaa cagcacacat caccgctgtc aatacgatca
     1621 agagtatcga tgcaaaagtc ccatgcgaac gaatatgtgc gagtagctct gccttactcg
     1681 ctctcggatt taaaaataat tggatgtctg tctgatattc taaatacaac cgatagccca
     1741 ctaccagtat cagaatgatt cccaacgaga tcaacatcaa tcggattatt ttctttttca
     1801 tatttcctcc tgaaaaaaat ctatattcat taaataatag tttaacataa aaacaaaaaa
     1861 gcaccgaaat ccaacatagg atctcagtgc ttttaaatag taaaaattat ttaacagtta
     1921 cagatgcgcc aacttcttca agtttagctt tcaattcttc agcttcttct ttagatacgc
     1981 cttctttaac tggtgcagga gcgccatcaa ctacagcttt agcttctttc aagcctaaac
     2041 cagttgcttc acgaactgct ttgataactt taactttttg gtctccagct gcagttaatt
     2101 ctacagtgaa ttctgtttgt tcttcagcag cagcagcgcc accagcagca gctacagcta
     2161 caggagcagc agcagatacg ccaaattctt cttcaatagc tttaactagg tcgttaagtt
     2221 ctaggatagt tgcttctttt aactcagcaa cgatgttttc aatgttcaat gccattataa
     2281 tttcctccgt ttaattgttc ttttttattt aatgctttat gcggcaatta agctgcatct
     2341 ccatcttctt tgtctgccac tgctttgaca gcgtaagcca cgttgcggac tggcgcttgt
     2401 agtacagata gcaacataga tagaagtcct tcgcgatttg gcaattttgc caatgctgtg
     2461 atttcttcca atgaagagac tttaccttcg atgataccgc ctttgatttc taatgctttt
     2521 gcttctttag caaattcgtc catgattttc gctggtgcga ctacatcttc gttactgaat
     2581 gcaacagctg taggtccagt gaatacttcg tccatgcctt caaggccagc ttttttagct
     2641 gcacgtgaaa gaatagaatt tttgataact ttcatttcta cgttagcatc acgaagttgt
     2701 ttacgtaaac gtgttacttc atcaactgtt aatccgcggt agtcaacgac tactactgat
     2761 gcagctgctg taaatttttc gcttacttca tcaacgattg ctgctttttt tgcgataatt
     2821 gcttcactca tttttgagtt tcacctcctg aattttgatg gaagagtttt ttgcactgaa
     2881 aaggcaagtc acttttatag ttactttgct caaccagtat tattcatcac ctattcactt
     2941 cgttcatagt gattctactc gctttgctcg aaaggaaact cctttcgctt tgctcaacca
     3001 gtattgttca tcatctcttc acttcgttca tagtgattca caataaaaaa ctctatgcca
     3061 ccgcagacat agagggacta ttgaatatat tcaataaacg tcctcggtag gaaattaagg
     3121 cttgatgcca cctactgtct tcggtacaag ttaattatta acctcaacta gcttagcatg
     3181 ctaaactagt tgagtcaaga attatttttc agttggatca atcaacattc taatatttaa
     3241 ttagaaagaa gcttggtcaa catggatacc aggtccaaat gttgttgtga tcgttacatt
     3301 tttcatgtat tgacctttag ctgctgatgg tttagctttt agcaatacat catggattgc
     3361 tgcaaagttt tcaactaatt tttcatcgtc aaatgaaact tttccgattg gtacatggat
     3421 atttccagct ttgtccacac ggtaagttac tttaccagct ttcacttcgt ttactgcttt
     3481 tgttacgtcc attgtaactg taccagtttt agggtttggc attaagcctt ttggtcctaa
     3541 tactcgtcct agacgaccta cagtagccat catatcaggt gttgcaacaa ctacatcaaa
     3601 gtcaaaccat ccgttttgga ttttttgaac catgtcgtcg tcacctacga aatctgcacc
     3661 agctgcttca gcttcttttg ctttgtctcc tttagcaaaa actaaaacag tttgtgtttt
     3721 accagttccg tttggtaata caacagcacc acggatttgt tggtctgctt ttttagggtc
     3781 tacgttcaaa cggtatgcaa cttcaactgt tgcatcaaat tttgcaatgt ttgtttcttt
     3841 tgctaaagct actgcttcgg caacaggata aactttactt gaatcaactt tttttaatgc
     3901 atcttgcatt tttttgctct ttttagccat tttggttcct ccttgattgt ggttataacg
     3961 gttgtacctc ccacgtgttc tatatctttc aatatagagc caactgagaa gctgctgtct
     4021 tagggtgtat agattattct acagtgatcc ccatgcttcg tgcagttcct tcaaccatgc
     4081 gcattgctgc ttcaacgtta gctgcgttta ggtcttccat ttttaattca gcgatttctc
     4141 ttacttgatc gctagaaact ttcgcaactt tatttttgtt tggttcgcct gaaccttttt
     4201 cgattttagc tgcttttttc aatagaacag cagctggcgg tgtttttgta acgaatgtga
     4261 atgaacggtc ttcgtataca gaaataacaa ccggaatgat caaacctgct tgatcagctg
     4321 tacgagcgtt gaattctttt gtgaatccca tgatattgat acccgcttga cctagtgcag
     4381 gacctactgg tggagctgga gttgctttac ctgcaggaat ttgcaattta acgatttttt
     4441 ctactttctt tgccacgaga catacctcct taagtccgtg atgtggttaa ttggagatgc
     4501 atatttctcc tcccacgttt tatctgatct gaaaaaatcc agacaaataa tgtgcaaaac
     4561 aatgcacact ggtacagtat agcaaattta tttgtttttt caagattttt ttctgctatt
     4621 tttacatttt gcttgaataa acgctaaatt tattctaaaa aaacaacaat ttttcgttct
     4681 catttcttct tttatgttac acttgcgcta gcatggttca acagatgaaa gcaaacaata
     4741 acacggtttt ttagaccctt gtacaaagat tcctacataa caaaatgagg tggagaaaat
     4801 gaaagattta aggtatagaa gtgtctttga tattattgga cctgtgatga ttggtccaag
     4861 tagctctcac actgccggag ctgctagaat cgggaaaatc atacgtagta tatttggcga
     4921 acagcctgat tctgtagata tttatctata tgaatcattt gcaaaaacct atcgggggca
     4981 cggaacagat attgcattag ttggcggcct gttagggatg gaacccgatg atgaacgttt
     5041 agctgattct ttgaaattgg catatgaagc cggtatggaa gtcttgtttg ttcctaagag
     5101 tgaaaaagcc gaacatccaa actctgtaaa gatgcttcta aaaaaaggaa cacgaaagct
     5161 ttcagtcact ggcatttcga tcggcggtgg caatatccag atttctgaat tgaatggatt
     5221 caaaatttct ttaagcatgg gtactcctac cttgatcgtt gtccaccaag atgttccagg
     5281 tatgattgct aaagtgacta gtatactttc tgagaccaat acgaatatcg gcactatgac
     5341 agtgacacgg gaatcaaaag gtgaaaatgc cattatgata attgaagtag atgatcctca
     5401 agtagaagaa acggtaaaaa gactcaaaca gctgccaaat atcgacagcg taaattattt
     5461 cgattaagga gctaaaaaaa tgtttttatc gattgaagaa ttagtagatc aagcaaaagc
     5521 ttatccaagt gtagcagaat tgatgattgc agttgaaatg gaaatgagtg gccgttcccg
     5581 tagtcacatc atcgagatca tggaaaaaaa cctctcagta atggaacaat caatcaacga
     5641 gggaaaagaa ggtgttacct ccgtcacagg aattaccggt ggtgatgcaa aaaaattaga
     5701 tgattattta aatgaggcag actttttaag tggagaaaca atcttaaaag ctgtacgcaa
     5761 tgctgtagct gttaatgaag tcaatgcaaa aatggggttg atttgtgcta caccgactgc
     5821 cggaagcgcg ggtgttgttc ctggtgtgct gatggctgtc cgagatcggc ttgatttgac
     5881 acatgaacag caattggaat tcttatttac cgctggcgct ttcgggttag tcatcgcaaa
     5941 caacgcttct atcagtggtg cagaaggggg atgtcaagca gaagttggtt ctgcaagtgc
     6001 aatggcagca gcagctttgg tttgtgcaaa aaatgggact cctcatcaag cagcacaagc
     6061 agtagccatt acattgaaaa atatgatggg actgatttgc gatcccgttg caggcttagt
     6121 tgaagtcccg tgtgtcaaac gtaatgcttt aggctcttct caagctttta tctctgctga
     6181 tatggcatta gcgggtatcg aaagtgtcat tccgccggat gaagtcgttg ctgcaatgta
     6241 ccaagtcgga cgtcaaatgc cgtcgatttt caaagaaaca gcagaaggtg gcttagctgt
     6301 tacgccaact gctaaacgac ttgcggaaga gattttaaat aagagggcgt gataaagctc
     6361 cttaatttcg agtattaaga gattagttat cttcataaca aaaaacgaag agaggactaa
     6421 ttgcccttct cttcgttttt tattaatcca tcaatttcgt attttttact tcatcgatcg
     6481 tttttgttcc tgcaagttgc atcgtgatcg acaattcttt gttcaggtgt tcaaaaacag
     6541 atgttactcc ttctgcccca cctagattca acccataaat cactggtcgg ccaatcgcaa
     6601 ctaagtcagc accacttgcc agagctttga agacatgctc tcctcgacgg acaccagagt
     6661 cgaaaatcac tggtacacgt ttgttgacta cttcggcaat ttttggcaat acttcaaagg
     6721 acgcaggacc tccatctaat tgacgtccac catgattgga tacccagata ccatctgccc
     6781 ctgcagagat agcgattact gcatcttctg gcgattgtat tccttttacg ataactggaa
     6841 gatgggtgat ttctttgatt gttttgatgt catctggtgt caagccttgt tttgcagctg
     6901 cataaatttc tgcgattcct tttccttcgc cattaccact tgcactttgt tcgctatacg
     6961 cagctaagtt aggcattggt aatggaaatt ggaattgatt gatgacgtct tcttcacggt
     7021 aaccaccgag cgttgaatct gccgttaaaa tgatagcttt agcccctgct tctactgctt
     7081 tgttcaaaat gaactcattg aatctgtcgt ctttactcat atataattgg aaaaactgcg
     7141 gtgcgcctgg cgctgcatcg gctgcgtctt tgattgtcgt atttgcataa gtgctgatgg
     7201 aaaagattga tccagcagca gctactcctt tagctgtatc agcttcccct ttttcatgtg
     7261 ccaatccttg ggccgctgat ggcgcttgga taatcggtgt atctaattca attccgaaaa
     7321 cagatgtatg caaatcggct gagtcgattc cgcgtaatat ccgtggcatg attttctttg
     7381 tattaaaaga agtcgtattt tctttcatcg tccactcatc ttcagaacca ccgcgaatat
     7441 agccaaaagc tcctgcttcc atgcgctctt ttacacgacc ttctagttag gcaatgttta
     7501 caatatcgat tggatgttct tcattacttg tatgataagt catagtagat ccttctttca
     7561 tcttcttgat tacatataaa tcatactaaa cttacaaaaa gtaagcaaac atttactctc
     7621 agatactttt atcgctattc tttccaaata aatcgactta caagctgatc tacttcttgg
     7681 ttttcatgaa gcatactatg agaggcattt tcacctgtca ctactttttc ctctatttca
     7741 tttccgcgtt gcttcaacaa cgcatagacc gctaatgcac tgtttaacgg tactgttcca
     7801 tcactcaagt ctgtttcatc tagttgacca gcaatcaaga aaaatcgagt ggttatagga
     7861 atattaccga tcatttgctg gtaatcttga tagcggttag aaacgacagc aggccccttt
     7921 gctaactcat ctgtcagaga ctgcgcacta tcatcgacaa aatcattaaa aggcgcgcca
     7981 attgctgtta atgtctgaac ttgtggcaat gacgtatcct gtccatacgt tcctaaatag
     8041 cgtagtgcac taactcctcc catggaatgc cccactatgt tgactttatc tatgccgtag
     8101 ttttctttca gatacaaaag acaagttttg atccattctg cttgattcca ctcgttattt
     8161 ttattatctt caaacaatac ttggaccatc ggattgtctg cttttccaga caaatctcct
     8221 gttgcttgta tttgtccatc tgttcctact gttaaaacca attcttttac tgcttgctga
     8281 tttttttcca aacgccgcaa catcccttta aaggaatttt ctgtcccgct gtagccgtgg
     8341 aaaaaaagtg ttggaatagt ggttttttct atcactttac tggattctgc cgttttttct
     8401 ttatcagaaa cttcagtagt atttatcttc gtcgtctggc aaccacttaa cgcaaacaaa
     8461 ctgataagga ctaaagctgc tgcagttgca cgtttgcata aatttttgat tggttctgtc
     8521 atacacctct tcctttccat atagaaaaaa gaaagacttg aaaggccaat catatgatcg
     8581 acatttcaag tctctttatt aatcgatttt atctacttga tcgaaatcaa gttctgtact
     8641 ggtttcacgt ccaaacatat cgatgttgac tttcaatttt tctttttctc cgtcaacttc
     8701 tgttacttgt ccttctaatc cagcaaatgc gccttcgatg attttcacga cttcgccaac
     8761 ttctacttct agatcatttt gtcgcaggct cataccgata gaacgcaaga tatgattgac
     8821 ttcttctgga agtaatggtg ctggtttgct tccggctccg tgtgaaccaa cgaatcctgt
     8881 cacaccaggt gtgttgcgga caacatacca tgaagcgtct gtcatgatca tttcaacaag
     8941 tacgtatcct gggaatgttt tatgaacgat ttctttttct ttcccatttt tgacttcagt
     9001 ttctttttct tcaggaacaa cgacacggaa aataaagtcg gccattttca tactttgcgc
     9061 acgagattcg atatttgctt ttactttgtt ttcatatcct gaatatgtat gcaagacata
     9121 ccagtttttt tcaaaagatt ccattgaagg ctcctcattt cattgtaaaa taaaaaaacc
     9181 tcagtacacc cgaagttttt ctttcgcttc attatagcac taaaagtacg ttgtggctag
     9241 cgagttattt taaaatccag ccaaataagc tttggatggc tgtatccatt acgtaaaaca
     9301 tcaatccgaa aataatagaa gtttcgatga ctactaacgt atctttacgg agttgttttc
     9361 tgcttggcca agttacttgt ttgatttcat cttttacgct ttttagaaat ttcatgagac
     9421 aacctccctt gctactgttt atttcgtttc tttatgtaat gtatattgat tacaatattt
     9481 acaaaactta ttgatttcca aacgttcgcc gctttttcct tcactgactg attttgtgta
     9541 gttgcgagac ccacaaacgg aacaagcgag tgctgctttt ttctttgcca tttacttctg
     9601 cccccttgct gagaatcaga cttatacttc ataacagtat cacgtaactt tttacttgtc
     9661 aatttgtgaa ataaactatc tattgttttg gataaaatta tgatatgatt aatttgaaat
     9721 taagctaaaa tgagacaatt ggaggtatag cgatgctatt aaaaacactt gtatataaga
     9781 aaaaagatct gacaaccgtt acagagacta gtacattaga agaagcatta aaaattttag
     9841 aaacttccgg ataccgttgt gtgccgatct tagattcttc aggtaatatt ttccgtggaa
     9901 acatttacaa aatgcatatc tatcgtcata aagcaaatgg cggtgatatg acgcttcctg
     9961 tcactcatct tttgaaaaac gctacaaagt tcatttacct ggatacttct ttcttcaaag
    10021 tattctttac gatcaaagag cttccttata ttgctgtcct tgacagtgaa aatcattttt
    10081 atggtatttt gacacatagt acgttgctta atatgctctc tcaatcttgg agcgtagaac
    10141 aaggaagcta tgtactgaca attgcttcca caggaaagca aggagatttg gctgctattg
    10201 caaaaatcat ttcaaaacac agcagcattg ccagctgtat tactcttgat atcgaaaaag
    10261 acgaatacat tcgacgtaca ttgatcacgc taccggctaa cactgaagcg aaagtatgtg
    10321 aaacgatcgt caaccactta gagaaaaaga attataaagt ggtagaaatt gaaaatttgc
    10381 aaaaccaaga agcataatca aaaagcgaaa aaagaaaaca gctaatctgc tttctttttt
    10441 cgctttttat aattagacac gcttttcaaa atcaagcgcg cgatctaaat aatacatcaa
    10501 tggtgctgaa acagccatga cgataaactc acttaatgca agcgtgccat aggttgtcca
    10561 aaatgcttgg ccacctgttg gaactaacat ataagcaatc agtcccatgc tgatggtaaa
    10621 gaagatcgtg ttcaatacta aacgcatctt tacatttttc actttgtttt gcaataaagc
    10681 tgtcaggcct aaagaaagca atgtttggcc gccaccaaaa accacatcca tgataccaaa
    10741 accaaagaag gcattataga cgatgacccc gcctaaaact ccccaaagca atttacggtt
    10801 aaagacaact aaatgattca aactttctga aatacgaaac tggatcggcc ctgaggatac
    10861 aggcgcaact aaaatagtta acgctagata aagagccatg atgatgccat ttacaacgat
    10921 tacggataac cgttgtctgc ttttcgtttt ttgcattttc tctcctcttt tctcctagtt
    10981 tttttaacgc gggatggttg atgaaccgcg gttatgtgaa acaaacaata attataacag
    11041 acttttcatt tatttcaatc cgtcttatct gcttttaaaa aagcaaaagc ttaaacaatg
    11101 taaacgtttg ctaaaaatcg tgggatatgg tataaagtaa gtgtaagggg tcataagaat
    11161 tcccagtaaa gcagacgaag taggatctgc aagaacagaa ttgttgtgaa acttaaataa
    11221 ataaaaaagg atgtgggaat ttgagcaacc tcttactaga gaacggtcta gcaagcagct
    11281 gaagatctaa gtggacatgc cgaaccttgg aacttctcct tacgtaagta atggatcatg
    11341 cgcttgctag gcttttttgt gtttttcggt cttcggacct ttttcatttt cctagacttc
    11401 tgctataata accaagaatt aattttgtaa cagatttttt tgaaaaaaca agtttctttc
    11461 attcaaaaaa tagaaaaagc ttgctaatta ttggttaacc agcaaacagg agtattaaaa
    11521 tatcttgttt tattgaatag caggaggaaa tcatgaaatt ccattatcat atattgatca
    11581 atccatcggc aggcagcgga aatggctata aagtagcaga aagaatttta ccggtattaa
    11641 aaaataagca tattgactat acacttcatt atagtgaata caaaggacac gatgctgaga
    11701 ttgctgaaac tttggcaaaa gaaacattga ttccttggat agaagaagaa cagaaaacaa
    11761 tcgatacttt cccccttttg atcatcgtag gaggtgacgg caccttacat caggtacttg
    11821 atactttcta tcagatggaa gtagaattcc cagttgctta tattccagca ggttctggaa
    11881 atgattttgc tcgaggggca ggccttccaa aaaatcccaa aaaaggactt cagctgattt
    11941 tagcagctca atcaccagaa aaagtccaca taatggctta tgaagaaaaa ataagtgaaa
    12001 aaaaaggaat agctgttaat aatttcggta taggattaga tgcagctatc gtccatgcga
    12061 caaatcattc ttccacaaaa aagcgattga acaaatacaa cttaggatca ttttcgtatt
    12121 tattctctat tctccgtgct ttattcactc aaaaaggatt tccaattctg gtagatgcag
    12181 gaggaaaaca attaagtttt tcaaacgcat ttttatgtac agcaacgaag catccttttt
    12241 ttggcggagg aatcgcgatt gttccaactg ctgatgcgtc taaaccagtc attgattttg
    12301 tcgtggtcga acgaatcaat ttctttaaaa tcttgtggct cattctttta cttatacaga
    12361 aaaaacaatt gaaatcgaaa tatttccatc actacacaac aaacagatta cgtatcgttt
    12421 caacaacacc gcaacatggt caagaagatg gagaagaaat ggaaaaacga ccttttgata
    12481 tcacaataac cacaactaaa cagttgtttt ggtgttaata aaagaggttt tgtcagaagc
    12541 tacatctgga acaccgttta ttcggaaaaa aggactgtga cacgactttt gtcacagtcc
    12601 tttttttact atgcagttaa ttctttgccc aattgggttg ccatcgatag tttctttctt
    12661 tacccaaaat aaattggatc attgaaaaca ccgcagcaaa agcaactaag acacaaccat
    12721 tgaaccaaaa agattctgca aatgtttgat tgtaactgag tgtttcactg atcaagttat
    12781 tattttgaac aaaccaatat gttggtgtga atgccgctat tttgctaacc atttctggca
    12841 agtattctgc tggtacaaac accccaccga taaagcaact gcctgtgata aaaatatctt
    12901 tgatcccacc gctggtttct gagtttttta ccaatgaagt gatacaagcc gaaaagctaa
    12961 tgatcggcaa taaaaacaag aaagtattta aaatatgata ccataccgtt atatcccacg
    13021 aggtgtggat cactaacaga accattagaa gtaagaagcc actgaacaac agcagcgagt
    13081 aactaatcaa actaaaggtt actttgcgat tcaagtttcg tttggtaatg ggcgcactct
    13141 ggtttcgttt actgatctct ttgcgattaa acgctagatt gatgacgctg aaaccgctaa
    13201 aaatggatag aaacaaccca taagacaaga aattaaaggt tgtgcccgtc aacagctttt
    13261 tcatccgctg actatatccc gcacttagct caacttctcc agtaaccgag agtgccattt
    13321 gtgtctgttc catagctgca tcgattgttt tatcttcttc ttgaaacaga atcagtgttt
    13381 gcaaaaaggt gttcacttgc tgtgtaacaa ctgttttaga aaaggcatct gggcgactct
    13441 gtatcttgat cgtcgccatc tttcctactt cgacttgttc ggtaaagtct gcaggcagat
    13501 gtaagataat gtttacacga ttaaaataaa gagcatcatc gatttctttt tgtgaggttc
    13561 catccaatgt aacgactgtt tccttgtcat tcaaatattt acgaaggcca tcgactacag
    13621 gcgactcttc ttcactaatc aaggcgattt tcgcatcttg cagagaggta ttttcaacat
    13681 caatattttg cgcaaaaaag aatgtggcaa caacagtgat aattacacct atcagtaaat
    13741 tcgatttatt tgctttaagc aatgtccaaa tcgttttata aagcgtcata acgcacccct
    13801 ctttccatcc aagcatttcc taacgcaaaa aggaaaataa atccgatcaa ccatagaaga
    13861 ttggtataaa aactagccaa atcagtgtag aaataaagtt tatacaaact ttcgctgatc
    13921 aagttgacga gatttaattg accaacaatc ggaatatgaa gatcaatcca atacttcact
    13981 tgttccgacc ccatcatacc agcaaaaaag ctcatcgcaa tcgtcactga aatcaagatg
    14041 ctatcctttt gcaccagatt catttttggt atcaaattac caaccaatgt acctaacaaa
    14101 atcgcacaca acgaccccag agcggtaaca atcatcaacc attgccaacg ttcaccaaag
    14161 tctacttgat agataaaacg aaagacagct aacataatat aaatctctgt caaaacaatc
    14221 gtaaagcttg ctgccaaatt agcgattgaa actaacgatt gctttctagg aatcaaggac
    14281 aagcgaatac catttgacga ttgatttgct tgctgatcct gtacattgcg gaccccccac
    14341 atcgtcccgt agagaatcgc cattccaatc agagtgaaga aataaaaact tttgaaactc
    14401 atattacgac ctgcatgaac ttcttgaaaa gatacttctt gttcaaaaac agtcatgatg
    14461 ggcgcgagat caacgttctc cacttctgcc actttactcg aaattatacc aatacgctgt
    14521 aaaaattgat ccatgacgtt tttcatcaac gtctgactca ttcctgcttg acccacatgg
    14581 agcgaaacag atgaaccatc aatgagataa aaaccagcca aatcatctgc atttagcttt
    14641 tcattcgctt ctacttccga tagttcctgt acttcaaata atggtttatc attgacttcg
    14701 acttgttcta agactgtggt aaaattttcc gaaagcgctg tttgatccgc tttgattaca
    14761 attccagtat taatcgtttc aagattgcca ccttgatcaa tctcgccaaa ggccgccata
    14821 aacatcaatc ctaaaagaat tggaaaggcc aatgtccaaa acaacatgga tttatttcgc
    14881 agcagtattt ttgtgcgata tttataaaaa tgccaaaaca ttatttcccc tcctctagtc
    14941 tctcaaatcc ttgccagtaa tctctaaaaa gacatcattc aaggacgctt ctttggttgc
    15001 tatttttccg taactgatct tttcttcttg taagattgta agaatcggca acaatactgt
    15061 atttagttca gtagttgtta cttggagctg acctttttta taagcgattc catcagaagc
    15121 tattgttcgc tgaatcgctg acaacacatt ttctgggagt aatgggatgt cgatcgtcaa
    15181 acgatccgcc gtccgtacca tatcttttag agattctttg gttcctttag caatcacctg
    15241 tccttgatcc atgatcacga tctggtcgca aagaatttct acttcttcca tgtaatgtgt
    15301 tgtatagaca atggttgctc cttgtcgatt cagctccttg atactttcta aaattttatt
    15361 ccgactttgg gggtctacag ctactgtcgg ctcatctaaa aaaattaacc gcggcttgtg
    15421 ggcaatccca catgcgatgt tcaaccgacg tttcaagcca ccactaagtt ctcctggacg
    15481 aaacgaaaca tatttttcta atcccactaa ttgaatcacc tcttcaatca aggctttcct
    15541 tttctctttt tcggaaacat ataaaccgca aaaataatcg atattttctt gcacacttag
    15601 ttcgtcaaag accgcaacgt cttgaggaac gaccccaatc attgccttga cagcataatt
    15661 ttcaggagtc attgttgtcc cgaacaattt aatggttcct ttgtcataat ttaacaaaga
    15721 aagcatacag ttgatggcag tcgatttccc actgccgttt ggacctaaca gacctaagac
    15781 ttctccttct tgtacttgca gatcaaaatg attcaatgct acaaaatctc catagcgttt
    15841 gactaattta tcaatttcga taatcatcat gaaaaactcc ttccaaattt cttgttgatc
    15901 tgactataat aaagtgcaag aaaagaaact agtgacgatt ggctaaacca cacatgacat
    15961 ttgtcacaat gaaggagtct gctatgcata aatctcggct actcaacgaa actagctttc
    16021 tactggttat ctcattgctt ttgctggtaa gtcaaggatt cgttgctatg caagttatct
    16081 atttattgat cgccgtttta ttcactggtg tcagttattt actgcctaca aagccaaatt
    16141 ggattttcca agttattcta tttcttttat gtgccttttt acctgatctt gtcctgtttc
    16201 tgccagcgac gatgcgagtg atcgcccctg aagaaagcca ccttgattac ttgtttttgt
    16261 taggtttttt gttgatttcg acggttggta atcatccccc catcaaggtc gcctttgtcg
    16321 taattttgct gtttttttcc ttttacttac gaaaaaaaga tcaacgaacg accgatcttg
    16381 agaaaaagta cattgtctcc aaagatactc attgggaaaa acaacaagag ttatcggaac
    16441 aaaataaagc attggccctg tcacaagaaa cacaattagc cttgcaaatt gtggaagaac
    16501 ggaaccgaat tgcccgagac atccatgaca atgtcggtca tctgctgtca agtgcgattt
    16561 tacaaattgg tgcgcttgaa atgatcaatc aagaaccaaa actaaaagaa ccattagaaa
    16621 atttaagtgc caccgttcac actgggatgg atcggattcg tcaaagtgtc catgacctcc
    16681 atgagacatc gatctctttt caacactcct tagagtttct gatcgctgat tttccttctt
    16741 ctgttacgat tgaaggaaac ttgtttgaag gattgaatga aacgcagcaa aactgcttct
    16801 taatggtcat caaagaagcc ttaaccaaca gtatcaaaca tagtcatgcc aaaaccgttg
    16861 ttttacattt tcgaacgttg ccggcctttt atcgcttgca aatcacgaat gatggcacca
    16921 gcccacaaaa aaattctgct cccggtattg gtttgaaaac gatgcgtcaa cgtgttcaag
    16981 aacaaaaggg acaagtccat attttccaag aaaaccagca atttcagtta acgatcattt
    17041 taccaaggga gggacaccaa tgataacagt agtcattatt gatgatgatc catttgtgac
    17101 gaaatcgtta caaaccattt tagaaagcac gaaagagatc cgtgtgttag gcattggtca
    17161 ttgtgccaag gatgcgctag tcttatacga aacccatcgt cccgatgttt tgctgacgga
    17221 catccgcatg cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc
    17281 caaagccatc cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt
    17341 ttccatcggt gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt
    17401 caaatccgct tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca
    17461 aatgatgaag aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga
    17521 gcttgcaatc atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt
    17581 gtacttgagt gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt
    17641 acgggatcgc acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa
    17701 cacaaaaaaa cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt
    17761 caattgtccc gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca
    17821 ggattcgttg gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt
    17881 tctagttttg attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat
    17941 tctttttcca tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct
    18001 aatttatctt cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca
    18061 tatcacactg aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat
    18121 ctttaaaatc aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca
    18181 atcatcggat ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa
    18241 acagcaggtt ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt
    18301 tccactgttt ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt
    18361 agcttttctt ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt
    18421 ccgaccaaag aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca
    18481 ttatccatga tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa
    18541 gtgacatagg cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc
    18601 gtgtagagca aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc
    18661 atcttgaatt cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta
    18721 ttccattcgt ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc
    18781 actttatctt cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg
    18841 aatacattca attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg
    18901 gtgtcgttcc aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc
    18961 attttattta acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata
    19021 tcttgtgcac ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca
    19081 atttgataag catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt
    19141 ggcagacgtc cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc
    19201 cgataaatct gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt
    19261 acttgaccag gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata
    19321 ttcgtcggaa tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca
    19381 tcaacaatca aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt
    19441 ccattcagcc ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt
    19501 cgtttagctg ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat
    19561 tttgaaggat ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc
    19621 acagtagttt tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct
    19681 tcagcgatag cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc
    19741 cgaatattct tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg
    19801 cgctggatcg aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta
    19861 gtctcttctt gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga
    19921 tcaggctgga tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat
    19981 gtctccgccg gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag
    20041 atagctgctc gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta
    20101 tctgcacgtt tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg
    20161 atgatatcca aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg
    20221 aacccgtaac gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc
    20281 tcagcaagca tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga
    20341 tcagcaatga ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt
    20401 ttcttcccga ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt
    20461 gttggctttt cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc
    20521 actaattctc cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg
    20581 actacaatct ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact
    20641 ttgtaaaaat tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc
    20701 atttcttctg gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt
    20761 ttcagttcct aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca
    20821 aggctcttca gtctctaaaa tcgtcaaact actattgaac aatccaccca tttcacgcaa
    20881 ctcacttagc tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc
    20941 tacgaataaa agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat
    21001 acgtgtgatt gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa
    21061 atgattacgc aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg
    21121 accttctaaa gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg
    21181 acgagcaagc tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga
    21241 ataaattgct ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta
    21301 actttgtggc aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca
    21361 ttgtgtcttc ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt
    21421 ttattttact ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa
    21481 ttctaaatta gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt
    21541 cgataaattc aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc
    21601 gttttcacgc ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg
    21661 ctcttctcca tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa
    21721 atgtagttta cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat
    21781 atttacatca ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa
    21841 acctagtggc tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt
    21901 tccagtgttt gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc
    21961 tctcatattt atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt
    22021 atcaactcgg tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt
    22081 tatcagaaca ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc
    22141 ctcaactttt ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag
    22201 ttccttttaa taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg
    22261 ctttgattcg ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat
    22321 tttcctctgg tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt
    22381 cagcaaactt ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat
    22441 aaaaagcctg acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc
    22501 catcttctga gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa
    22561 ccgcgttgaa tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg
    22621 cttcggcaga ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag
    22681 cattttgaat ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa
    22741 catctaatag cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat
    22801 acatgccaga ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt
    22861 taaccgcatg ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat
    22921 ttttgcatat caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga
    22981 acagctgatg attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc
    23041 cccctcattt ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg
    23101 atacaatgcc tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg
    23161 cgctgaaaac tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt
    23221 gtgtgacaac ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt
    23281 aagttatgct ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga
    23341 cttcgtctat aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa
    23401 attagggacg gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga
    23461 ggccttagaa ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt
    23521 tgacagtatt cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt
    23581 tgaccgtatc ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg
    23641 gcttaaagaa ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt
    23701 cactgcagag aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac
    23761 caaaatcgtg gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa
    23821 tgcttattgc aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata
    23881 ttgaaataaa tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct
    23941 actgctggat caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc
    24001 gccactgtga ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt
    24061 ttccaaaaac ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg
    24121 gtcaagactt cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca
    24181 agtcgacgaa cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc
    24241 agcgaaatga agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt
    24301 aaagaagcgg tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac
    24361 gctgccttta gtggattttc attaatttct tccacattga ccccggcaag tcctgaataa
    24421 tcgctagcag cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac
    24481 aaatgatcat cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt
    24541 gccacatctt cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca
    24601 gaaatcattg cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc
    24661 tgaccttctt gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc
    24721 ttcagcacgt acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt
    24781 gtcatgatcg cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca
    24841 tcgtctgccg acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc
    24901 aagtcaacag cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc
    24961 atttcatcta ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc
    25021 gctaattctt taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat
    25081 tggtagaatt gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga
    25141 atattatttt cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt
    25201 tcatccaaac caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt
    25261 ctgcggctaa aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca
    25321 gatcataaca atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat
    25381 catcaccgag cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc
    25441 ctgtatgcag ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact
    25501 cagtctgtgc cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc
    25561 gggcgatcgg caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta
    25621 aggcctgata ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg
    25681 caaatccatg ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca
    25741 catggattac ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca
    25801 ttgtcccatt tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta
    25861 ccagaacgtg ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt
    25921 catgttctcc ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca
    25981 agacattttc tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta
    26041 acagaccctt tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt
    26101 tctcataatc ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga
    26161 tgccaaaaat gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa
    26221 taaattgagc ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct
    26281 tctgtatttt ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc
    26341 aaagtacgaa acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata
    26401 ctaggatgga tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta
    26461 gaaatcgtca atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg
    26521 gctaccatcg tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg
    26581 tccggtgttt catccaaata actgatccga acatccagca agggataact aacactttgt
    26641 tcccggttcg ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg
    26701 taaaacccta aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg
    26761 tagtgatgga acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc
    26821 tcatgatcat ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact
    26881 tctatagtct ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct
    26941 ttcgctttct ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg
    27001 ggagaataac cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa
    27061 ctgataggca gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc
    27121 ttgatattta taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg
    27181 aatctggatt tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat
    27241 caccagatga taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg
    27301 gagaactttc atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac
    27361 aaactgacac atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat
    27421 tggaatatgc cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt
    27481 gacaaattca atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag
    27541 tttcactttc aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg
    27601 ttccatagct gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa
    27661 gttttttcgt actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca
    27721 aaaaggagtc gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa
    27781 gcaagagtac gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca
    27841 gactaatatt tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg
    27901 gatccgtttc gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg
    27961 tctcgaaaca acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg
    28021 tctgcctcgt tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc
    28081 attaatccaa accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact
    28141 tttagctatt gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt
    28201 aggggatgca tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc
    28261 ttatcgtcct gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt
    28321 cattttcaca aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg
    28381 aaagaatcta gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat
    28441 ctatttattt gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca
    28501 atttatcgaa gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc
    28561 tttggtcaat ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa
    28621 tcttgaggaa cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc
    28681 ggcgcagttt caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt
    28741 cggctgtaat gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg
    28801 cggcgatttg aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt
    28861 ttctgatcaa aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta
    28921 caattatgcc tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct
    28981 ttggagaatc acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga
    29041 acatttacat acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc
    29101 taattaaaaa
//
LOCUS       DAZUMK010000021        28882 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000021 DAZUMK010000000
VERSION     DAZUMK010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.021"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1514)
                     /locus_tag="ACG808_000952"
     CDS             complement(<1..1514)
                     /locus_tag="ACG808_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5046598.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFL"
     gene            complement(1570..4050)
                     /locus_tag="ACG808_000953"
     CDS             complement(1570..4050)
                     /locus_tag="ACG808_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046599.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(4034..4369)
                     /locus_tag="ACG808_000954"
     CDS             complement(4034..4369)
                     /locus_tag="ACG808_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046600.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(4383..5417)
                     /locus_tag="ACG808_000955"
     CDS             complement(4383..5417)
                     /locus_tag="ACG808_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFS5046601.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(5507..6187)
                     /locus_tag="ACG808_000956"
     CDS             complement(5507..6187)
                     /locus_tag="ACG808_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFS5046602.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(6471..7574)
                     /locus_tag="ACG808_000957"
     CDS             complement(6471..7574)
                     /locus_tag="ACG808_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFS5046603.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(8203..8673)
                     /locus_tag="ACG808_000958"
     CDS             complement(8203..8673)
                     /locus_tag="ACG808_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFS5046604.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            9003..10643
                     /locus_tag="ACG808_000959"
     CDS             9003..10643
                     /locus_tag="ACG808_000959"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFS5046605.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            10797..12119
                     /locus_tag="ACG808_000960"
     CDS             10797..12119
                     /locus_tag="ACG808_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFS5046606.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(12187..12621)
                     /locus_tag="ACG808_000961"
     CDS             complement(12187..12621)
                     /locus_tag="ACG808_000961"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5046607.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(12686..13522)
                     /locus_tag="ACG808_000962"
     CDS             complement(12686..13522)
                     /locus_tag="ACG808_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFS5046608.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            complement(14264..14890)
                     /locus_tag="ACG808_000963"
     CDS             complement(14264..14890)
                     /locus_tag="ACG808_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046609.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(15185..15520)
                     /locus_tag="ACG808_000964"
     CDS             complement(15185..15520)
                     /locus_tag="ACG808_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFS5046610.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(15507..15791)
                     /locus_tag="ACG808_000965"
     CDS             complement(15507..15791)
                     /locus_tag="ACG808_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFS5046611.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(15847..17370)
                     /locus_tag="ACG808_000966"
     CDS             complement(15847..17370)
                     /locus_tag="ACG808_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFS5046612.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(17363..18538)
                     /locus_tag="ACG808_000967"
     CDS             complement(17363..18538)
                     /locus_tag="ACG808_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS5046613.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(18542..18727)
                     /locus_tag="ACG808_000968"
     CDS             complement(18542..18727)
                     /locus_tag="ACG808_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046614.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(18693..20387)
                     /locus_tag="ACG808_000969"
     CDS             complement(18693..20387)
                     /locus_tag="ACG808_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFS5046615.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(20384..20857)
                     /locus_tag="ACG808_000970"
     CDS             complement(20384..20857)
                     /locus_tag="ACG808_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFS5046616.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(20926..21081)
                     /locus_tag="ACG808_000971"
     CDS             complement(20926..21081)
                     /locus_tag="ACG808_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HFS5046617.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(21215..21595)
                     /locus_tag="ACG808_000972"
     CDS             complement(21215..21595)
                     /locus_tag="ACG808_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFS5046618.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(21599..21814)
                     /locus_tag="ACG808_000973"
     CDS             complement(21599..21814)
                     /locus_tag="ACG808_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046619.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(21817..22224)
                     /locus_tag="ACG808_000974"
     CDS             complement(21817..22224)
                     /locus_tag="ACG808_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HFS5046620.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(22490..23965)
                     /locus_tag="ACG808_000975"
     CDS             complement(22490..23965)
                     /locus_tag="ACG808_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFS5046621.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(23955..24803)
                     /locus_tag="ACG808_000976"
     CDS             complement(23955..24803)
                     /locus_tag="ACG808_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFS5046622.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(24840..25202)
                     /locus_tag="ACG808_000977"
     CDS             complement(24840..25202)
                     /locus_tag="ACG808_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046623.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(25203..25361)
                     /locus_tag="ACG808_000978"
     CDS             complement(25203..25361)
                     /locus_tag="ACG808_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046624.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(25502..26126)
                     /locus_tag="ACG808_000979"
                     /pseudo
     CDS             complement(25502..26126)
                     /locus_tag="ACG808_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(26123..26434)
                     /locus_tag="ACG808_000980"
     CDS             complement(26123..26434)
                     /locus_tag="ACG808_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046625.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(26478..26771)
                     /locus_tag="ACG808_000981"
     CDS             complement(26478..26771)
                     /locus_tag="ACG808_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046626.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            26965..27612
                     /locus_tag="ACG808_000982"
     CDS             26965..27612
                     /locus_tag="ACG808_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5046627.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            27673..28818
                     /locus_tag="ACG808_000983"
     CDS             27673..28818
                     /locus_tag="ACG808_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5046628.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
BASE COUNT         8412 a         5898 c         4902 g         9670 t
ORIGIN      
        1 tttaaaaatt ggttcaaata ggggaaatca taattatatt cgttgctagt gaatacaaaa
       61 actttagcgg acttctgtga cgggtactct ttcaattggc tagtcaaaat gatatcatac
      121 tgcttttctt tctcgtattc gactaaatca tatttgatcg tattcagcaa ttgttcctgg
      181 atgatttctt tcacgatatc cgccattagc ctttcataag gaagatcata agcaatttgc
      241 agacgtaatt ctaactgctt gatcagcgtc cgcaaaatag atgcatagcg agcaaataat
      301 tcaaagcggg cattttcctc gtctacttca gtaaaatcaa caatagcaaa tgtttgtctg
      361 accaatcttt cacataaaac caggaattgt ttatcttcta cttcagttaa ccgctgtttt
      421 aatccttctt ggccaaaaat attcatccat cctctaaaat aggcaaaatg agcgtgattt
      481 tgcatcactg tcaacgcaat cttttgcgaa atctcttctg gaaaaacata aaaagagaaa
      541 tattcttcta tcgattgttt aaacgttcga ttcaattgag ctactacgtc gtctttgtat
      601 ttttcgctta tcaaaaactc ctggacatgc gagttatgtg tatctaatgt aaagttactg
      661 atcatgaaaa gataaaaaac caacgtttca ttttgattcc aaggaaaggc atattgaaag
      721 aagtaacggg tcaaaaatgt atggaggtcc aataaaaatt gtgtcgtttg aaacaatcca
      781 acaggaagat tgctataatc tggtttggat tttttcgtac agcgcattcg cttcttcata
      841 atggaaagcc atatttcaaa tttcaatcgt ccttcttctg taatagtaaa actaaactct
      901 ttttgcagca gatctaaaaa agtgtttcct gataaatcac tgtattcttt tttgttttca
      961 agataaggac gtccaaacca aaataattgg aaataaaaat aacaaatttg actttctgga
     1021 ccggtcagcc gcccgctttt tattttcaga tgaaactcat ttaaagaatc atttaattcc
     1081 ttcactctgc gagacaaagt agccgaactg atattaagtt ccatgacaag tttctgccga
     1141 tcaaatgagc cattttccaa cagataaaga agtatctgat attttgtaga atagcgcaag
     1201 tagcaatgat aataaacatc gactgctgct atatttccat gtctgattaa taaaatattc
     1261 ccttcttgga taacaatttc caattgtttg atattttcca agttagcttg taaataaaac
     1321 agatcttctt tcaaggtagc ccgatcaact gataagcggg taagtacgtt attaacggaa
     1381 atctctcctt tttcctctaa taaaagttgg aaaagtttga tttgccgttg ttcatttttt
     1441 tcaagcaaac tgaaaagttc caagtccact cctccaagca tttctgattt taacagcaga
     1501 agtttccttc tcatccttga aaaagaggat ctgtgacagt tatcacagat ccatacaaat
     1561 tctaatcact tagctggttg gcccagctgt cagttcccat gtgaccatgc cttgatacgt
     1621 tgcgccaggt tgagcattcg tactgctatt tacctctaag agtaaaccat cggtatcggg
     1681 aaagtcaacg agaacctctc ctttagctgt tccatcactc tcataaatca acgtgttttg
     1741 accttgctga atcaattgtt ttgatccaga atgattgaag tataattttg ttccgcttaa
     1801 ttcttgacct tgatcattct taaataaaga agtcaatgtc cctgtcagtt gccaaggaga
     1861 cgtgcttgga agccgagtat cccgcacatc aaaagaaaaa tcgccttttg cttcataata
     1921 acttgtcttc aacggtttgg ggagatcgtt accaaatgta aaatcatctg gtacattaac
     1981 taatgttaat tcttgtttgg ggacaagaat tgttttagca gatgtttgga caactgggtt
     2041 aggaagctta gcaccatctg ctaatgtaat attgctggaa agtacagctg cctttgtcag
     2101 tatgctgacg gattgtacag aagtatctac agtatttaat tgatagttgt aagtaagatg
     2161 aatggtatct cctattttgc tcaatagatc tgctgggaca gaaactcctt gttgaatgcc
     2221 ggtatctgcg atagttgtac catttaattt taaggaacca ttgatataag tagtattttc
     2281 aggaagtgag atcgtgattt ttttgtcggt tatcgcatcc ccagattttg ttaacgtgaa
     2341 tgtcacattt tcttgaatcg tatctcctga tagagcagac aggggatctc ctgtctcagg
     2401 ttgtctgcta aggtttttta gctgactatc tgcactcaca gatggaaaat tagccactgt
     2461 tatttgagga agagtggtga ttccagcttg ttcagactgc catttgtacg caaaagacgg
     2521 atacatgtta gtaagtacat cgtccaccaa gcgataatca aaataatatt gccctgaagc
     2581 caatgcaggt agtgtagaag tagctgtatt tacacccccg ttatttgatt ggatcgtttg
     2641 gaaaagttga tctgtccatc ctgtcgctaa tttttgggtt cctttcgaat aaagcctgta
     2701 atggatcgaa ccctttttta gttcagttgc tgaccaattg gccgtgactt ggtacgatgc
     2761 agaagaaagg agctttcccc actgactatc ggctgttaag tttgttggta aaaccgttcc
     2821 gtctagcttg tactgctcca atacctttaa agcaacagga gaaagcaatc cgccttgcgc
     2881 atctttccca ctgtacacga tattggctgt ctgattcaat ccacttgtgc ttgctggagt
     2941 atgagtcagt attgctcctt gtgtaacgtt ttttcctgta aaatagttga ctacgcgatc
     3001 aaagcgacta aatggcaact gatcaaaacg tccttcagtc agccccatcg tcaatgtccg
     3061 actattggta gagcctggaa ggttctggtt atttccatcc acgtattctg tggtatcagc
     3121 aaatgtatta tattttcctg ttgaatcact tgttgagatg taactattcc acgtatttat
     3181 ctctgcactt gcagaaaatg catatgctcc tggattcaag tttccgccag ttaatttagg
     3241 ataaaatcta cctgcagctg gagctaaaat cgaattgaac atacgatctt ggataatatt
     3301 attcgaatca taaaaatcaa cagaaaaaac ggccttttta gacatagaag gttgtaattt
     3361 acccttggtc aaaattttgt ttggactcgt atcatattta tctccgtttt ctgtcatctt
     3421 cgcgacatta gctgtaacat caaattgaag cgggatatcg tccacgttga attcgcctcg
     3481 cgtaataata gcaataatat ctgccaatag agtatttgta tcccacgacc ccatcccaaa
     3541 acgcaaggta tggcttcctg ctgataataa actaccgcta gtaaccttga tgggggcacc
     3601 ggcaatatac aagtaggcgt tgttccagtc aattgctttt aatacatcac tgcttgatac
     3661 atgttccggc aaattgatga tcgccacaaa tgcaaatcct tgtgcagtga tattcaccaa
     3721 atcaagtaca tttgttagtt tcacatggct ttcccaatga aaacggaccg tgccaccttt
     3781 ccaaggatca tttgaaccat cgttcaaata agtagcagtc aatttaggca attggttagc
     3841 cccagttagt tggacaagct tcccgccatt gactgcacta cgtccaccat ttggaggtac
     3901 gatattttca tacgtgtatg gatcataaga aatggcagga ggcgttgctc caacagcttc
     3961 tgccgaaggt gaaaagataa atatacttaa aacaaaaagc gaagtaaaaa agaaggagcg
     4021 aaaaaatgaa aatttatttt gtagcaacat gttcatcctc cttttccttt cttctccata
     4081 acaacaacca cgcaagaagc cataattcta taccaatgat caaataaaga aaacggatct
     4141 tttcttcatt cgtctccggg aatacgccat gttgattaga ttctttataa atcggtagct
     4201 gtgtctgctg ttttgatgcg ccattgactg aaccacttgg atgcggaact ggacttagat
     4261 cataaaatgt aatcgtacct gaacttttgg tatctgcttg gactggctct tgccagctga
     4321 agaacagcaa acttgcaata atccctccca ataagatcga tgttttcaac gaaatcacct
     4381 catcatgttg tttctccttt tttctttttc ttgtggaccc acaaaaacag caaaatcaac
     4441 cataacacta gaactgcagc aaccaatcct gcataaagcc atgggaaaga ttgttcttgt
     4501 actgcaaaaa cggaattttc atttactttt tgtgcttttt cttttttgat cgtaaagttt
     4561 tgtgtaaatc gccattcatt actgtcgcct gattttgcta tgatttctgc gcggtaatcg
     4621 ccggcttttg ctttatcacc agctaaatca agattcaagt gaaaattcga atttggtgca
     4681 actaaaaatt cattagacgt attttcaata taaagctgat tttcataaaa gatcttgctc
     4741 gtaaatatca aattgctgat aatcctaggc tgaggatttt ggaaacgaag ctgaacagtt
     4801 ggctttgcgt tgacctcgtc caaatctcct gataaaagtt tcagatccgg ttctatcgtc
     4861 tcttgacttt cttgtaaaac gactgcgaga ttataagcaa tacgattttt gatttgtgct
     4921 ccttctgtag cattttctgt ttcagcttct tctttaggtg aaacatgaat acccgctaat
     4981 acacgtccct taaagctttt tgctgggatt ttcacttcta taggtatagt tgtttctgca
     5041 tgtgctgata cattgatttc ctttttcgca atgagcaaaa cgtcacggac atcaaacggc
     5101 aatgtctcat ctaacgttgg gtttttttga ctataatcga tcgttccacc atcactcgtt
     5161 accgccggat tgacagagac ctcaaatgtt cgttcttcat ctgaagaatt gctgataatt
     5221 acttctagtg tctgattttg ttccggtgcc actaataaat caaaataacc gattgcttta
     5281 tctatttggt tctctggaaa taccggtttg attccaaagt ctcctgctga atcctttgct
     5341 tggctgattg acggaaaaat gaaaaataga cttaaaatag aaaaaaacga aacaaacagt
     5401 cgtttacttt gattcattgt ccaccacatc ctttcaacaa caactgcggt aaaaaatcct
     5461 tcacctatag tattttcacc gcagtttgtt gaatttattt catactctat gctactgggc
     5521 cagccacgag gttccaagtc aaatcagccg tgaatgttcc agctgctgct ttcggcactt
     5581 tcagtgtgat atttgctgga tcataaacag aaagccatgt tcccagacct tgatcttttg
     5641 ctgctgtaaa tattggtgtc gctgcacttg ctttactcaa cccagttaca gtttgactag
     5701 ttggtccagt ggattcgtta tctgacgtac ttttaacagt ggatgttcca aaatctaatt
     5761 cagcgccttg caaaacttgt gaaccgttta caaaatctga aaccgaaact gtcacgttcc
     5821 acccttgtgc ttggccatct gcacctacac ctcgacgatc tgatacttgg agataaggtg
     5881 tatcgttttt tgaagcgtct acttgatacg tttttgttcc gctttcaatc tcgtgtgtac
     5941 caaatggcaa ttcaggaact acatccaatg ttaaagaacc tgtctggcca gttgctggat
     6001 tttccggatc tgctggctct actggttttg aaggatcagt agggttaaga ggctcaacgg
     6061 tatttgttgg ttcagtaaag gtaatatctc cttttgaagt cgcttgaccc tcagcaaaag
     6121 ctggtgttgc caagccgcct agaagaagtg ttgaaagtaa taatgtccca atcatgttct
     6181 ttttcataat aaatcactcc tttttcttag tgacctaagt atagtagaga cagtatgcaa
     6241 aaaatcctca tgaattcatc cgcttacttg aaattatgac cctttttgta taaaagagaa
     6301 aaaaacaata aaatcgttta catgaatatt cttccttttt tattaagtat gaaaaaatat
     6361 ttcaatttta ttttttcgtt tcaaattatt ctattagtga catccttgaa ctataaaaat
     6421 aggagtacgg catcatctgc cgcactccta tttttttcgt catttatttc ttaaataaaa
     6481 cgattctcac gcaattttat ttttttgata tcttctactg tttgtgttcc agctaattgc
     6541 atgaccattt ccaattctgt tttaaagaaa tcaaacactt gatggactcc tgtacttcct
     6601 cctaaagaca atccataaat aacgggtctg ccaatagcaa caagatcggc acctgatgcg
     6661 attgctttga aaacatgttg tccacgtcgt acaccgctgt caaacacgat tggtacacgt
     6721 ttatctactg cttctgccac atattgcaac gaatcaaacg cagcaggccc cccatctaat
     6781 tgacggccac catggttaga tacccaaatt ccaccagcac cagattctaa tgatcgatag
     6841 acatcttctt ctgattgaac acctttcacg tagactggca aatcagaatg agcagcgata
     6901 aattctacat cttttggact aagtttttgt ttggaagatt tatatacagc atccatcgtt
     6961 tggcctactc ctgattgata agcctgaacg atcggcattg gcagcgggaa ggtaaacccg
     7021 tttctgcggt ctgtttcacg gtttccgcca actgttgcat ccgctgtcaa tacgattgct
     7081 tttgctccat tacgcttggc cacttcaaga atatccaaat tgattccatt atctttgctc
     7141 atataaaatt gaaaccattg cggtgctttc tccccgcctg cttctcttat ctcttctaaa
     7201 gtacaagaag cgtaagaact tgctgtatag atcgtcccaa aatctgctac accttttgca
     7261 gaagctttct ccgccttcac atgagctaat ccatgagcag caactggtgc catgatgatt
     7321 ggtgcagtca gcatttcttc gtcaaaatgt gtagttgtat cgggtaattc tacatctctt
     7381 aatacatgag gaataatcaa acgatgattg aatgcccgtt cattttcttg ataagtaaat
     7441 aagtcgccag caccactgct gatgtaacca taaccaccag ctggaatcac ttgtgaagcc
     7501 gcgatttcta aatcttccat attgataaaa tctactattc cttcatttgt acctgcttga
     7561 tatgtttttt ccatctcaat gccttctctc tataaaattt gaatgatgaa caatatgata
     7621 gcgtttacac tttttatatt actcttcgac attgatacag gcaaataatc tgtttacatt
     7681 tcagccattc cagatgtcac tactctggaa gaaatgattg taataaaaat agataaaaaa
     7741 ccgatgggac tttgacaaaa gtagtgtcaa agtccctttt tttgaataaa cggtgttcaa
     7801 aagccatttt ctcggtatta agtcaaattt atgcaaaaat gtggaaagcc attttcacaa
     7861 aatttttctt actactcgaa actaagcgac tttttcacaa cctctcgata aaacggtgtt
     7921 caatcagctg accctcggta ttagctcaaa aaatggcaaa atatgaggaa ctattttgcc
     7981 atttgtctct ctaatactca ggtcagaacg ttgacttcac agtctcttcc ttaaacggtg
     8041 ttcaaaaact tgcatctgcg gtaataagcc caaccaaacg aaaaatagga ggagctattt
     8101 ttgtttggct gttcttatta cttgatgcag ggcaggtttt tcacgacctc tgattcacgg
     8161 tgtttctttt tcatcaacat ttttacgaaa tactgtggaa gactattgaa ataagtgtgg
     8221 attgtttctt tgtgtttcaa tcgtttcttg ccattcttca tttaattttg gattaccgtt
     8281 ttcttccttg aactcataag gtcctaattc aaaaatccca gacagtattt gattcacgat
     8341 ttcatatagt tcagtgtgat cagacacatg attcttgaag atcatctcat tatcaccacg
     8401 tttgaacctc acattcgggc tctcatgatg aacctctatg catttttctc ctttttttaa
     8461 ctgacggact tctgaaaaca tctcataaat atcttctcca gtaaaacctt cagcaagcgc
     8521 cagactgtcc acaagaccgc ctactgtcag ttcagagatt attgcttgat tggaatctaa
     8581 ttttaatgta tagcttgtcg cttctactac gccacatgca ttgcaaccgc cttctgatag
     8641 ttcgcttttt ctaatcaatt gaacttcttt cattttctct tcctcctctt aattttctac
     8701 ttatgtattt ttatcacctg acataggtga ctcctatcaa cgctatcatg tgcttttttc
     8761 ctcagaaatc ctatttatcc attccatcta tcccttattc ttctactttt ctttctctag
     8821 catataagag atacgcatag tattcaagca aggattgtcg atataaaaag aacaaatact
     8881 gctattttga tttcttttgg ctatattaca tataaataaa atttatcata cctgctcttt
     8941 ttgactatat catcatctga cttctatact tcgtttgtat taaaacttag gaggaatcat
     9001 tcatgcttac agggttaaac ttattaaata atccttttct aaataaagga acagctttta
     9061 caaaagaaga acgggcaaaa tatggaatca ctggtatgct tccaagtact gtccaaacac
     9121 tggaacaaca atctgttcaa gcttatggtc aatacttaag caaacaaaca gatttggaaa
     9181 aacgtatctt cttaatgaat ctattcaata cgaatcggac attgttttat aaattgatgg
     9241 gcgaacattt agttgaattc atgcctgtcg tttatgatcc tgtcgttgcg gattcaatcg
     9301 aacaatacaa cgaaattttc ttgaaaccgc aagatgctgc atttctttca atcgacgaac
     9361 cagaaaacat caaagcaagt ttgaagaacg cggcagatgg acgagacatt cgtttgattg
     9421 ttgtgactga tgcagaagga atcttaggta tgggcgactg gggtgtgaac ggtgttgaca
     9481 tcgctatcgg caaattgatg gtctatactg ctgctgctgg tattaatcca gcacaagttt
     9541 tgcctgtatc gatcgacgct ggtactaata atgaagaatt attgaataat cccttatact
     9601 taggcaatcg tcatgctcgt gtagaaggcg aaacttacta tgaattcatc gatcaattcg
     9661 ttcaatcagc taccgaatta ttcccagaat tgttattgca ttgggaagat tttggacgag
     9721 gaaatgctgc agcgatctta gaaaaatacg aagatcaaat cactactttc aacgacgata
     9781 tccaaggtac aggaattgtt gtcctagcag gtgtattagg cggattgaat atatccggtg
     9841 aacaattgaa agatcaaata attttgacat ttggtgctgg tactgctggt gtgggtatcg
     9901 caaacatcct tttagatgag atgatccgtc aaggtgtacc tgaagaagaa gcacgccaac
     9961 atttctatca agtagataaa caaggtttat tgtttgaaga tacagaagga ttgactcctg
    10021 gtcaaatccc attcgctcgc aaacgctcag atttcactaa tgctgatgaa ttgacaaact
    10081 tagaagcagt tgtaaaagcg atccatccga caatcatgat tggtacttct acccaacctg
    10141 gagcattcac tgaagaaatc gtgaaagaaa tggctgctca tacaccacgt ccgatcatca
    10201 tgcctttgtc taatccaaca aaattggcag aagcaaaagc aaaagattta atagaatgga
    10261 cggacggaaa agcattagtt ggaacaggta tccctgctgc tgatgttgaa tataatggtg
    10321 taacttacca aatcggtcaa gcaaataatg ctttgatgta cccaggttta ggacttggtt
    10381 tgattgcttc aacagctaca cgagtaaatg ctgaaatcat ttctcaagct agtcgagcat
    10441 taggcggaat cgttgatgta acaaaaccag gagcagctat cttgcctcca gtagcaaaaa
    10501 tcactgaatt ttctcaaacg attgctgaaa ctgtcgcaaa atcagttgtc gcacaaaact
    10561 tgaatcgtga agaaatcacg gatatcaaag aagcagttga atctgctaaa tgggttcctg
    10621 aatataaatc tttagaagat taagatcgtt atagtaaaag aacatacaga aactttgtat
    10681 gacgtctaaa aagaaaaaag cgactgcggc aaaacagcgg ccagccaact cctgctcttt
    10741 tgtcgctttt ctttggcttt tttcatatga tacagaataa gaaaggatct gtaaaaatgg
    10801 cacaaaaaac aacaactgca actgcagttg aggaaaagaa aaaagaaggt ttttggtcca
    10861 caaaaatcac cggcgtaagt ttgccacttt atttgatcat ggtccttgtc ctgatcattg
    10921 caatggcttt agggaaatta ccgacgaata tgattggacc aatcgcagtt ttggttattt
    10981 tcggtaattt atttcatttt atcgggacaa aaatccctat cgtcaaaagt tatttgggtg
    11041 gtggttccgt ttttgcgatc ttcgcttctg ctgcgattgc tacatttggg atcttacctg
    11101 aatatgtagt gaaatccaca gaaaactttg tcagcaatat gggcttttgg gacttttata
    11161 ttgctgcctt gattactggt agcatccttg gtatgaaccg taatctattg atgaaagctt
    11221 ctgttcgttt tattcccgtt gcacttattt caatggtcgt ctcctttttt gccgtaggac
    11281 tcgtgggcat gcttatcgga aatggttttg caaattccgt tttatacatc tcattacctg
    11341 cgatggctgg tggtgttgga gccggagccg ttcctttatc aacgatttat gcaaatgttt
    11401 taggtacgga tgcttcttct atcatttccc gcctgattcc tgcgacagca atgacaaatg
    11461 tcttagctat catcggtgca gcattagttg ctcgtgctgg acaatcaatg ccaaaattca
    11521 acggcaatgg taaattaatg gaaaaaggcg atcttggcga tggaaaacct cgaactgtga
    11581 aaccttccat tccacagtta ggtgttggtt tgatggtatc attgactttc ttcatcttag
    11641 gacaaattgg taactatttt gttcctaaag tccatgctta cgcatttatg attattatcg
    11701 ttgtcatctg taaaatcgcc aacctattac cagagtatta tgaagatgca gctatcatgt
    11761 tcaataactt gattgtcaaa aacttgacag cagctgttct tgccggtatc ggtattgcgt
    11821 tgctgaatct gaatgtatta gcttctgctt tgacatggca atttgttgtc ttatgtctga
    11881 caagtgttat cgcgatttca gttgtttcag gatttgtcgg tcgtttgttt ggtttatatc
    11941 caattgaatc aactattact gctggcttat gcaacaactc gatgggcgga acaggaaatg
    12001 tagccgtctt atctgcagct aatcggatgg aacttattgc ttttgctcaa atgggcaatc
    12061 gacttggtgg tgccatcgtt ttgatcatct ctggtttctt gatgcaatta ctttcttagt
    12121 caaaaaaaca aaaatacctg atcatcaagt atcggcttgg tgatcgggta tttttttgat
    12181 aaagatttac aggattttca aaaatctttt ttgcatttta tcggaatgat aaccgatgtg
    12241 ttcataaaat tggtgtgctt ctactctgta ttctgcagaa ttaagcctta ttgtcgttat
    12301 tcctcgtttt ttcgcttctt cttctacgcc agtcatcaaa gcctttccga tgcctctttt
    12361 ttcagcatat ttagacacag caagtgccat aatattgaac attggatcgc taaaaatcga
    12421 ttcataggac tccgcttgta catagccagc tgctttttta gaagtaggat cttcataaac
    12481 caagaaaaaa tttttctctg gctccttcag cagtttcttc atttgtctca ccgtaacggt
    12541 taatggtata tcatacccta attgatccgc attgatttca cgtagtgctt ctgcatcttc
    12601 taaacgcatt tcccgtaaca tccaaatcac cagccaattt ctgatttttt aaaatgaatt
    12661 tttttatcat agtatactct ttttttcatt tagaacaata tgtttcagtg attttgactt
    12721 gcgttagtct agattctcct aatgtttgta agatgacaaa ggcattgtaa caattgacca
    12781 aaagaaccac gaggacaatt acttgaaaaa taaaggtcac tttttttgga gacaaaaccc
    12841 tgctaaatct tgctccccac aaacctccaa taacagcagc ggggataaca taaaacaaca
    12901 tagataaatc aaagcgcata aagctacttg ttaaagcaat cgttaccaat tttgccagtt
    12961 gagaaaagaa aatcgtagat agagaatata gtgttgcttc ttttattggc aatgcaaaca
    13021 tcagcatcaa caaagaaaca ttgactggtc caccaccaat acctaaaaaa ctagctaaaa
    13081 agcccagaac ccccccacat ataaggtacc aagtccatga cgtcaaatga aattttggct
    13141 gatgatgctt ggtgtagaag aacgcaaaaa ccaaggtcaa taccgtcaaa aaaatttgga
    13201 tcagctgcac atgcttctcg ttttcaaata actgtaaaaa aacttcgaat acaatgttgc
    13261 cgactactcc tccaacaaca gctccactag atacccaacc gacaatctgc caattcagtg
    13321 atttcccgct tgatacttgt cgcacagtcg aaacaatgga catcgtgaat accgcaactg
    13381 ttgagtaaaa agaaatccct gcaacagaat gggcgccgat aagatcaaga atcggtttga
    13441 tcaacacgcc tccacccatc ccggaaatag cgccaactgt gttcgccaga acgatcacca
    13501 caaaataaat cagaccaatc atcttgtatt tcctttctct tgtttatatc ttttagcgat
    13561 tgttactctt ttcttttatt atacagtaaa agaaacaaac gaggcttcga aagcttttct
    13621 actttttaag gaaatttagt gctaaacgaa aagaggttgt gaaaaagctg tcctgcatca
    13681 agtaataaga acagccaaac aaaaatagct cctcataatt ttcgtttggt tgaacttatt
    13741 accgcagatg caagcttttg aacaccgttt aagaaagagg tcgtgagcct gccgttcagc
    13801 tccgagtcac aaggaacaat ttcaaaaaac agcttctcat gtttgttgaa tattgtgact
    13861 tgtgaccgag gagttggcag gagaacaccg tttaaggaag agactgtgaa gtcaacgttt
    13921 tgacctgatt attagagaaa caaatggcaa aatagttctt catatttcgc cgtttttcga
    13981 gcgaatgccg aggggcagct gattgaacat cgtttatttg gaaaaaggga ttgtgacaag
    14041 acgtttgtca caatcccttt ttttatatct tattaattca ttcttttgtt ctattcttct
    14101 tcttccgcca tgaatgagaa acccatatca aagaactata aaggttatca aaacagcgtt
    14161 tatgactaaa tgaattagag ttatctatca ctatttttca agcgatttaa accaaatggc
    14221 atacaatatg gcatacaaaa acttcaataa gccgtggggg tttttaaatt tcttcttgtt
    14281 ctttaaaatc aatgagtgga gctacataat ttgaatccac tttttcaaac gtcacttttt
    14341 gcatcgacga atgttttctt tcccaagata acgtaaatag atttgactct ggacttataa
    14401 agtctacttc accttctagt attactttct tatgcaaata ttcctctaat aaatatagac
    14461 ttgctaaagc atacaacaca ttttccaaat ttgcttcctt ataatttgaa gaacgaccat
    14521 gttttactct gttatatatt ttccaccaat ctggtgtatc ttcaggccac ccatctccaa
    14581 aaggttttag tgaagtcaat gttaaagaat tcataaccct aacaccatta tccaaacaca
    14641 ttaattcgct agatttaata acagctttaa aatcagttat gcttttattg ctactgctat
    14701 caaatccgca aagttctttg aaaataatct cacactccga accaatcgct agtagtagtt
    14761 tttgatattc aatagagaat gttttgaaat tattctcatc gagcatcaca aaatctgtgg
    14821 tattaataaa ctcattttct aaatgtagat aatacttcca ataaaaattt gtaaactctg
    14881 ctttgtccat taaatcacct cttttgaatt ataacaaaaa aaacacgtcc acaacagacg
    14941 tgttctttga tttctacaca atcagcttgc caaaaccatc caatcagtcc tagcatgcct
    15001 gtattaaaat tgaacgttca tcatctatca tctttcttag tccagcatgt ttgcaaccac
    15061 acctacaatt ttcagtccaa caatagattt cgactagcta agtcttgcaa aattcaggca
    15121 gctaaccctt catttactac cttaattata ctaaataccc acaacgttta caagtgtaat
    15181 cattctaaga aatctgttta gctatgatcg ttgtataatc tttaaacgcc gttccttcat
    15241 tgattgaaat aattttataa ctcttgtttt tccaagctac tttcatatcg tttgttagtt
    15301 cataatcttg atcgtatcga ataataaacg tcaacgttcc ttcaagaatg gtacctacac
    15361 tagcaagcac gtcttttagc atttgtgaac gcacattagc ccaacattca aatacagttt
    15421 cttcactatc caccatctgc ccatcttcat cttttacccg ttttgtcttt ttaaacttga
    15481 ctctctcagt caattcattt acattcttaa tgattggcat aataagcacc tctcatttgt
    15541 tgtactaatg cttgtaccgt tactggtatc cgttcgctgc tggcttcttg acggttcaaa
    15601 taccaatgtt gagtaagtaa tgacaccgcc aaatcaaatt gctgataatt atccatatct
    15661 ttatccgttg cgctactatc aatagcactt tttatatatt cgcctgctgt atcaatcaat
    15721 tgttgaataa agtcatcatc aatagtgtga tcgatccgca tacttttttt tatgctatcc
    15781 aaaattacca ttttatctct cctaatgaaa aaaaggcagt gggataaacc gccgcccaat
    15841 atttttctat acttttggtg ttacaactgg cgtaaactca atatacactg aagcattttg
    15901 atcaattttc ttataatcgt tgcgaacgat cactgcaagt ccttgtgagt agtaatcgaa
    15961 tttttcccat tgagtcgtta cttgatttct acgagcaaca aagatagatt gtgcaatatc
    16021 ccccataatc atagggaatg ttccagcttt aggattggca aacaatgtat ctgcaatcaa
    16081 tactactggc ataccgaata atgatttacc actaggtgct gtcacatcgg gttgtaaaat
    16141 gtaacgtcca tctgaatctt tcaatgtatc taggtggtaa tatccgcttt ggtttagaat
    16201 taccatttta tttaatgctg ggtctaatgc cacattgtat agttgtttca aatcgtccaa
    16261 cgtagcagcc gttttcttag tgaatgtttt taataaatcc atgatatgct tattgtcagt
    16321 gttttctacc aatttcgcta actgatcttt gacctcttgt acaatattca ctgctgaatc
    16381 ttccacaacc tcatttgata aggcaatctt accagcacga gtttctactt tataatcaac
    16441 tgaagtaaac atttcagcgt caatatcacc aatttcagca agttcagctt ttgttgctaa
    16501 cactgcttgt tggttagttg ctactggata tttaccttgt ccatttgata ctgtttttac
    16561 tgttgcatat tgagctaagt tataatttga acgtttcaaa tcaaagactt caccgattac
    16621 ttcttcggga acaactgccg ctgcatttac agtagtgact ccatcacgca cttcgccttg
    16681 tgaacggata tattcttcat acccacgcaa ttctgttttt tcgttatcga ttaaagtttt
    16741 ttccatgtta ttactctcct tttgtgcttt agaagtggat tctaagctat tgtttgtttg
    16801 ttttgcttgg ttgttactca taaacgattc gtaagaacgc ttatttactt ggacatttga
    16861 tgaatcataa gcgggaacgg tcacgacact aatttcatta agtgctttga ttttatctat
    16921 tgatcgttca attgtgccat cttctttttt gtcgaatgaa tctaatccaa gcacgaaccc
    16981 aaaggacata gaatcaacaa ccccttttga gatattctca tacacatcat ttgaataggt
    17041 cgtgtcggtt agctctgctt caaaatgtaa tcctgtatca tcgacattca atttcaacgt
    17101 acctgctttg acactagcta gtggctttga ataatcgtgt ccatataata agaacacatt
    17161 tgataaatcc acctctttta atgcttcggg tgtaatcact tctacaaagc cgcctaaatc
    17221 ttttgacggt tgcccgaatt tcaacgcata gcctgaaata gttttgccgt ctgttggttg
    17281 tttttcattt tctttctcta ctttgggaga atcggctgtt agctcagctt cttctgtcag
    17341 ccgtttttcc ttttcttctt cattattcaa ctggtgtaac tccttcctgt tcttttaatt
    17401 gatatttctc caacgtatct aaatacgtaa agttcaaact tgctagtaaa cgatcgccac
    17461 catccatggg gggtagcccc attttcgacc gcccctcatt aatcgtgaga agtgaccctt
    17521 tgacctgatc caatacattt tttacttttg tttcaggatc agtctctagt aactgatcgg
    17581 aattaaagcg gatattggtt tcaagtttcg tatctaattc actcacaaac acattaaagt
    17641 aatgaatcag cgtactttgg atatactgga gattactttg aacggtgcta gaatgttcat
    17701 tttcaacgcc taaacgctca atcggtacac caaacgcctt ggcaatttgt tttgtgttcc
    17761 aatcattgga attgataagc ttcaaaacat cggtgtttat ttctaatgtt ttgtagtcca
    17821 ttgtttcatc aagaatgatc gttctaagag cattatctcc actacttgaa ccgttggctt
    17881 cttcaaactt ttcacgaatt gcgttctttg ctgatccgtc taaatctgac ttatgaacct
    17941 tcaaaatacc tgacccactg acaccacgag taaagaaatt gtgcaatgtg cggttgccag
    18001 cttgttgaat tttcagttca tcacgtaacg cataaagtgg tggtaatcct gttaaaccat
    18061 cttgggtgaa atatttgaag tgcaaaatat cgctagactt cacacgtctt tttttatcac
    18121 caatctgata agacaacgta ccattatcta attgagtaac cgttacactt gagttaggta
    18181 ataagtgaat ttcttctacc ttttcaccgt tgcgtttaat ctctgcaaag ctattaccgt
    18241 ttagcaacat attgaccgcc aaagcaaact ttaaatgcca accatccatt tctgaattag
    18301 gtttctcgtt tagtaacttc accagttcat tatcagcttg tggcatattg tttttgacca
    18361 attgaatcgg acttgaagca atatcactag caataattcg aacggctgca aacacatcac
    18421 tatttcttaa tgcacgaacg cttgtaaagc tggtggtgta atcatccgat tggattgata
    18481 ccacatgttg taggaaagga tctcccgttg tattttgtct cgctttaaaa aatgccatct
    18541 tttaacctcc cttctgttct gtatttagca tgaccgctac cccaataagc actatgccca
    18601 gcgctaacaa gccatagtag acatttgtta gtaaggtgat agcaaccaac acacacacca
    18661 atccaagaag taaaagaacg gtatgaatgt aattagaaag aaaattcttc acttgtataa
    18721 aattcattgt ctgctttcgc tccttccgtt gtggtgaagt aatccattgc aaacacatag
    18781 gcattgatta atgcagctat tggatcaatt ttgttactgt tctttgattt attaatctga
    18841 atgccgttgt tatccgtttt taaaatagcg ttgttcactg catgggtgag aatcgtattt
    18901 ttattgtgaa taacgttgcc ttcataaact tgttctctaa aagttcgggt cggaacgttt
    18961 agagtaatcg ttccttgtct tacttctaac attgggtagt tggctttttc agctttactg
    19021 ataagcgaat tagcgttata cgggtcataa caaatcccca tacattccaa atcattttct
    19081 tgaatcaaat cttgaataaa ctgaaatact tggtcatagt ccacgattcc actttctaat
    19141 tgagtaatgg aacactcacc agctttttct agttctcgat aaggcaaacc gtcgcgcttt
    19201 tctttgtctt gaagcccata tttggtggct acaaagctat gtgaatcaca ataaagttcg
    19261 ccgttatcta gcggtacgat ccatgaaacg ctagttaaat catctgtttt agataaatcc
    19321 actccgatat aaacgggctt gcctgtaata tcttgtggtt ctacttcggt tgcctgccag
    19381 tcttcagcaa tcatatagct gtcctcactc gcttgtctcc ataaattgaa gtttttcacc
    19441 aatacagaat taaggttatt ttgtttcaaa gcaaggttca catcatcttg aatggttgga
    19501 atcattactt ttttgatttc ttcactttca aaaatcggat tggctttgat ccatttctct
    19561 tgatcgtgaa tttcttcggg atcgtccaat tcccatatcg ctataaagta cctgtcggct
    19621 tgttcttcgc cttttagcac acgatctaac aataagtatt cctcgtacat aggaacgttt
    19681 aaatcaagcc cagcggtact aataacacac aataagccgt tcttttgttg ggtcattcct
    19741 gacttaatga cgttgtacgt tttacgagtt tttgcttcgt gccactcgtc aagaattgcg
    19801 gttgttggtg caaacccatc tagcgtgctg gtttcactag ccagtgccat agcgaacgaa
    19861 ttagagggca agtgctgaat cttagagttc ataattttca gttgtggtct taaaaactta
    19921 ctagacttga ccacgtttct aagtgaatta gacagcatat catagcccaa tttagcttgt
    19981 ttcaaagcgt tacttacaaa caatacttgc cttgcttctg ctggttcttg ttccataatc
    20041 aacgcattgg cagccatgcc acttgcgata tacgttttcc cattcttacg gctcatactg
    20101 gtaaacgctc gattgaatcg cctgtaattc ccttctttag tacgccagcc ataaagactg
    20161 cctagaatga atttttgaaa tcctaacatc ttgattgctt taccgtccgt agatggtagc
    20221 aactcaatga atttaatcgc tttggtggct tgttcttcat cgaacacata agggaagcta
    20281 tctgacttag aacgctccaa atcgcttata tgacgctcac acgcttggat aattttcttg
    20341 ggtgcaatca gcttacctga taggactttc tcaatgtaat cattcatgaa ttcatcaact
    20401 ctttgaacgg gtcttcgggt tcatcatttt taaccttgtt caaagctagt tttgatcgtg
    20461 attctaaggt catacctagt gaggtggctt gttttttcaa atcactcata gcttgtgatt
    20521 gaacccgaac cgctggattg gctttcttta ctccgttttc gcatgtgata aacgtacctg
    20581 attttcttat ctcggcttcg gctgttttca ttcgagcaaa ggctaaacaa taggctacca
    20641 atgaaccata atctgcttca ctcaatggat aatctttttt taatgttggt acgatacgat
    20701 tccattcact tctagcgctc gctgccatcc agtcaggggg cgtgatattt attaatggtt
    20761 gctgcttgaa caattcttct ctagcgtcca cacggattgc tttttcttca cttgatatat
    20821 tgccttttgt atcgtgtaat attttagggt gtcccatgtt gtcctccttt cagacaaaag
    20881 aaaaaggaac gtaagcaatt aagctcacat tccttcgttt ttcgatcaga atttttcttt
    20941 atctaattta tctatataaa tgatttttcc gttctgcatt tgaatggtca cttgaccaaa
    21001 atgaggtaac tcaatggtgc gctgtttccc tgttttatca gtaataacaa ttgtattttt
    21061 caataaatct cctactttca ttatatactt tttcacttgt gattacaagt gtaatcgtta
    21121 tttgtaatta cccttatgcc ttttcacaac ggttatttgt accaaaatga gtaggcatcg
    21181 attgtcagta agtttcacac tatgccccct taactcaaag ggtactcttt tctcgtcttt
    21241 gtctttcatt ggctgtcttg gaattatggt gtgcatggca taaagaacga aaattagttt
    21301 catctaaccg tctattccaa tcatctttta gttcaatttt gtgatctaca acatcagctt
    21361 tccttatcaa gccttcttct aggcaatcct cacatactgg atgatttaac cgatacaagt
    21421 atgaagcctt tcgccatgtt ctactttgat agaattgaaa atatttacct tcgctttgct
    21481 ttctttcttc acgcttaatt gccgtttgct ttgctttatg cttcccacaa tacttctgcc
    21541 tataatcaat caatgtttta catccagcat gattacattg ttttttaact gccatagatt
    21601 accaaccaaa gcgatctcgt tcgtcagaat catcttgttc ccattttgat ttgtattcaa
    21661 tataaggcga aactatcttc aagcacgttc cgtctttaaa attaacttta gtgattttat
    21721 cttcctcaaa actaataaag tctacatctt cttgagtgtt tgaatactca atgtgtttct
    21781 caccatcgaa gtatctaata ctttccatat gcataattat tctacctcca ataaattagt
    21841 tatcaattca tcatctgtta gtagcaaagc caagaactta tcatggatgt ttaaaaacaa
    21901 attatcctca ttcgatgtaa gctctcgttg tctcgctcga tcatataggt taagcagaca
    21961 attaaattca tccattagct cacctgtatc atcatagttg ccactatcta ccaatgatcc
    22021 cactactagc gattgacacc acgctactga ctcctgcagc caatcaggtt cttcttccat
    22081 tatctttaat acttctggat caaatgtgga ataatcttct gttaccaatc ccacactaaa
    22141 tgccagcgtg ttcattagaa accgatccat ttcatctgta tattgaaagt tcggtgcttt
    22201 gtttgttgaa ataatattga gcatttactt tcctccttga ttcgtaacac gtggtaacac
    22261 ctacgtaaca ggtacccgtt accactcaat cccaacgggg acaagggttc tagcgttttg
    22321 gtaacaggta acgggtaaaa tccagtaaca ttattcccca gcatattagc taactatttc
    22381 aaaagtttta aggaaataag gtgttacccc gttaccaaat tgctgtatcc cttggggcag
    22441 taagggtaaa ctagtaacgg gctaggtgtt actgacccgt tactacccgt tactttttgt
    22501 actttccagc tcgattttta ttttcatagg catttgactt attgtaaaat cctcgtcgtt
    22561 tatttctttt tggcagttgc ttccgttccc aaccaggaat actgctaata atcaaggcaa
    22621 tctttttagt ttctgcatct gatcgaccat tcaataaatc ctttgcttgt ttatcaaaaa
    22681 cagcttccaa aatatcagcg gtgagtacgc tatcaactaa ataaaactct tgttgcatag
    22741 tagctccatt atagaaatgc tgtaaatttt tctcattaca tttactgtca ggatagttct
    22801 gataataaga acgtctcgcc cacgctggag cttcttccca tttatacggg atttccattt
    22861 ccaaatattt agtgattgct tctttaattg gattctccat cttggcatcc tcttgatagt
    22921 cttttgcaat ttctaagact tctttatctt catcgggact aaaatagatt cgttgtcctt
    22981 tatcaaaaag cacctttgct tcagctaaca cttgaagaaa atattcatcc tttgttttaa
    23041 acggattcat ctttggctta ttcttacaag gcaaaggata aaaacggcgg tttcctgtat
    23101 catctttcaa atactgccca tcattcgacg ttccaataaa tacacattgc cttgcccaag
    23161 ctatgacatt ccgaccataa ggaggtctaa ttttatcgat cttggcactg atgaagtttt
    23221 ttatctgttc aattttagtt ttgttcattg aagataattc tccaagttca ataattacat
    23281 tgccttgaag ctgcatataa tcatctttct tttcacctag cgaatctaat gaatcggtga
    23341 aaaaatcatc cgtgtaaagt aatgctggaa cggtactctt accaatccct tgcggtccat
    23401 ccaaaactgg aacgatctca aactttattc ctggtttata gatacgtgcg actgatccga
    23461 ctatccaacg ttttgttact tctcgtgtgt aaaggttgtc gtctacccct aaaaggtcaa
    23521 taaacagtgt ttccagtcta ggctgttgat cccatttaac tttttcgata cgttctttta
    23581 ctgggttgaa aacattcttt ctagccacat ttactactgc tttgtgtatt ttgttgtcat
    23641 caaaaactac attgtagttt tcttcaatat atgccgttaa ggcatcctca aaactgtctt
    23701 cccattctcc agcggtacga ttgatccaag gagaatcttt taataggtga atgtatccgc
    23761 taaattcgtt ataagcaatt gttccagcta aattgctatc attggttaaa atcaattctg
    23821 cattcacaac actgttactt ttaatctgat cgtctttatt gcatcttaat cgtgacatcc
    23881 aggttttcaa ttcaatgata ttgtcttcac gctcgctggc ttcgggaaag tctaaaatac
    23941 tatctaattt atgcttatga gccatgagct ttccccctca aatctttttt aatcatcgat
    24001 cgaataattt gattcacttc tttatctttt agcggtggtt cattgtacgc atttgctgta
    24061 taaatcaatt ctgctaaagt ttgattatct acaccgtgcc atagtaacca accgcataac
    24121 ttagttaaat gatcgtttct actaccttcg ccagcgcctt gaacaatcat atctaatttt
    24181 tttgctgtcc ctgtttttgc tacgggtcgg tattgagtct ttatttcttg cggttttttt
    24241 agcagtaggt tcactagcca atcgggagct ggtgcaatat tatttagaga tacgttttca
    24301 ataggttcat atgctctccc aacacttctt gaaataatca tagttggcgc tattggaact
    24361 cctttagtga taatatcaat cccgcttaaa cttttacctt tatcatcacg cccgaagata
    24421 tcagcaaatg cacctgtttc ttgttggata attacaccat caggaatttt aaagaagaaa
    24481 tggattcctt tacctggtgt gatttccgta taagtagaag gaaatggatc atacattgaa
    24541 gcaagttttt tataattctc aacgccgttt cgtgtttcag aatgttgatc taggtcaact
    24601 accaccattt gatgatcttc aagcattaac ccaatatttc tagttgcatc ttccgtccac
    24661 cattcattaa tttgattaga atcgttagtt gcatcattcc aaccactaga gccttttatt
    24721 ggaattttag ttccaggaga taagggataa attgaccatc ccgacttttg taattccgtt
    24781 gctgcttgat aattatttgc caataaacct ctcctccttt ttcaaaatac tggtataaat
    24841 cattcagaca cctccatgag cttcaactgc tcaagttcgt atttcaaccc atcccgaaca
    24901 aattcagcac cttttaattg attctgatac tttacccatg tattaaatgt tttcaccctt
    24961 gcttcttgca tatcagtaat gtttaaccat gcggttaaaa tctgattttt tagctcgttg
    25021 atttctcgac aaattatttt gtgttctgca tgttttttga gtacatacaa aactgttagt
    25081 ttttcattga atgaatttcc aaagtaggtt ttcccgatat tattttctag ttcagcgatc
    25141 ccttgattaa tttcctctaa ttcaaggcta tcgagttggt ttaatagttt tgttttgtac
    25201 atctacttat gccctccagt tcttaattga tagtcgtact cgtcataagc agtgaagaga
    25261 tataacgcta atcctgcaaa gatagctatt tttgcaatta atggtaataa gccgaaaatt
    25321 ccgccgatta aaatgcttcc tataattaag tttcgtttca taatggttcc ctccgatttt
    25381 ttaatatggt ataatcggaa caaagaatat cgggttttat cccaattaag ttgcctttat
    25441 cctgtccgcc aagactgtta tggataaggc tttttctttg ttctcgatca tgttaatgct
    25501 cctaagttgt ctgcaattca gcagaacgat tataaatttt ctcactcact gaaaactgca
    25561 acccatattt ttctttaaca gtcattaatt ctactgtctc atctaaaata ggttcacgat
    25621 caaccaacat ttcaggagtc atatctttct tttttaccat tttggaatga ccataaatag
    25681 ttgatacagc tttattggct atcacattcg ctttaatgta gtctactggt tttggttcag
    25741 taatagcgtg gcttaattta gtcattgctt ctttttggtg ttctttgtct agcatgcgga
    25801 atacttgaaa gccttcaagt cctgtggact ggcgtagttc tttgatgatg tcgaaaatcc
    25861 agtcttgaaa tgcctcggct tcttttttac ggcttttgaa tgccatgcgg taaatatttt
    25921 ttttcgttga tgatgtttac atcttgtgtt ctgcccaatg aatcgatgac cttactagta
    25981 gtaaccccat ctttaggcaa agagtgaatg gctctagtaa cctgtttaag acctaacgct
    26041 tccgcaacat ctttcgccac tgcccaccat tcatcattaa tatcgacaaa tcttataatg
    26101 tgtccgttcc aaatttcagt tttcattcaa cgtcctccag ttcaagccct agcaagctcg
    26161 ctaacaactc aacttgctga tacaaaattt cttgatactc ttcaatagaa agtcgttctt
    26221 taaactctgc ttgtactaaa tcgttcaagc cacgcaatac tccgcttacc gttggttctt
    26281 gtggtagcaa tttttgatag tccatttgtt cgtacaaaca ttcaaacgtt tcttctagcg
    26341 ctttgccttc caagtttgct aattctgccg ctgtgtcttc tgtaagaatt gttgataata
    26401 ctttttcgat tgctttattt tgtgtcgatt ccattattat ttcctcctat taatcccttt
    26461 gcccccacgg cttgcgttta tttttggtat tcttccataa agcgatcaca ttcatcttgt
    26521 gaaattctta cgactccatc aactataatc aactttaaac cgttaggaac atatttctgc
    26581 atcatagtat tgtatgaaac gttcatatac ttagcagctt gtgccttgtt catatatttt
    26641 ggaaactggt actttttttc taataatgaa agtaacgttc cttgagtttg ctctacttgt
    26701 tgaacaactc gatcgtcaat aattttaaaa gctggatcat caagcgtgag tttgtcaatt
    26761 tttaattcca tatttatccc ccctcgttag catacatttc tatacaaaga ttttaaaaaa
    26821 atgcaagagg ctcttgaata catttctata cctcttataa actcaatata ccatcatacc
    26881 ttttcatttg caagaatttt aacatacttt tttgaacttt tatgtttcac acatatttaa
    26941 atcgtgatat agtagagaaa atatatgaat ggtggtgaaa atgtggctag aaaaaaatac
    27001 cccgaagcag tggagacagg acaaagaatt agactggcta gaattgaaaa gggactctca
    27061 atggaacaac ttggaggtaa attatcccca ccggccagta aaggtgccgt tagtaattgg
    27121 gaaaatggct ataatcttcc caataacacc aggcttaaac aactttcaaa ggtattagat
    27181 gtatccacta cctatctatt aaacggatct tatacattaa gagatatgca tatgatgccc
    27241 gaaattaagc gaaatgtgct tgaaaagcaa ttgaaaaaaa ctattgaaaa aaataactct
    27301 ataattgatt ctaatctttc taatgctatc aataacatcg aagaagataa cctgactgtc
    27361 tcacagaaga tttatttaac aaacgcaata aaatatctta aaacaggatc tcgaattgaa
    27421 tttcctgacg ggagtacccc aattgcttat ctcgcagctc ttctaaatac gcttttacga
    27481 tatgactcat taattaatga ttcagaaaaa aatacagatg agaaaaaaga gtatcaagaa
    27541 ttttatgaaa atgatcttct aaaagagttc acagctcttg ttactgagct aaataaacac
    27601 tattcaaaat aataataaac tgcctcctcc aaccgccccc acggcttgct acactatgga
    27661 gggaaaacta ttatggcaac attcaagcaa tatgaaacta aaaaaggtaa attttggctc
    27721 tatgaagcct atcttggaat gaacaaaatg actggcaaac ctgataaggt tcgtagacga
    27781 ggttacaaaa caaaaaaaga agcccagctt gccttgtcac gcttgcaagt ggactacgat
    27841 aaaaacggta ttaaaaaagc gaacaacgaa acgtttaaag aagtttatga cttgtggcta
    27901 gaaacatata aaaccactgt ccgagaagcc accttcatga aaactgaaat caagtttaga
    27961 aagtggattc ttccaaaata tggaaatctt cgtgtgaatg aagtcacggt aaaaaaagcc
    28021 caacaaattg taaatagctg ggctgaaact actgaccaat acaaggtact tcattcaact
    28081 tctaagcgta tttttagcta cgctatcaat cttgggatta ttgagactaa tccactagac
    28141 aacgtattaa tgcctaaaag aaccaccact aagcataaag aaaaggtcaa agtttacagt
    28201 aaagaacaac tgatcacctt gtttgattac ctaaacagca aacctgaaac ttatcgaaac
    28261 gactttgata aaacgttact acgctttctc ttttatagcg gggttaggat cagtgaagcc
    28321 cttgcattga attggtcaga cattgatttt caagaaaata cggtcaccat caataaaaca
    28381 ctaagccaat ccaaaaatgg ctataaaatc agtgatccta aaactgattg tagtgctggg
    28441 gttcttccac tagatgaaag aacaatttct atattaaaaa aatggcagat taatcagcgg
    28501 aaatatatgc tgacacttgg tgtaactgat ccgactatga ttttttgtgg tatctataaa
    28561 gaactaatca cacaccacgc tatttatgca agattgatta ctatttgtga aaaagctatg
    28621 gttccatttc tcggtgtgca tgtagcaaga cacactcatg cttcaatgct cttggacgct
    28681 ggagctacta tggtagaagt tcaaactaga ttacggcata caaaaattag tcagacgatc
    28741 gattgttacg gtcacttggc aaaagaaacg aaagaaaaga ctgttgaaaa attagtacaa
    28801 cacttaaata ttgactagat ggcatacaat atggcataca gtaaaaaata aaaaaaggag
    28861 aaaccctatc atatcaaggt tt
//
LOCUS       DAZUMK010000022        28742 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000022 DAZUMK010000000
VERSION     DAZUMK010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.022"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            602..934
                     /gene="traE"
                     /locus_tag="ACG808_000984"
     CDS             602..934
                     /gene="traE"
                     /locus_tag="ACG808_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFS5046629.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1553..1894
                     /locus_tag="ACG808_000985"
     CDS             1553..1894
                     /locus_tag="ACG808_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFS5046630.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            1913..2203
                     /locus_tag="ACG808_000986"
     CDS             1913..2203
                     /locus_tag="ACG808_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046631.1"
                     /translation="MKFFEVKSPYYYALIAAKNEEECAELYEQVVTDIENREEFIRCL
                     HELNRNEAIQEDAKKISEETLQPIGMEEATKDIFTIIDEQKSCVLSIDSTLV"
     gene            2395..2628
                     /locus_tag="ACG808_000987"
     CDS             2395..2628
                     /locus_tag="ACG808_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046632.1"
                     /translation="MARPVLDAMDMDTLTKGRYVQLLARGYSPKDVFKALSKGTDMEK
                     ERLRKEFDYWREHNGIKDLKPARPVIRRKKLKK"
     gene            2694..2933
                     /locus_tag="ACG808_000988"
     CDS             2694..2933
                     /locus_tag="ACG808_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046633.1"
                     /translation="MNCKEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYVKKSSNCQLLLEVS"
     gene            3019..3690
                     /locus_tag="ACG808_000989"
     CDS             3019..3690
                     /locus_tag="ACG808_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFS5046634.1"
                     /translation="MKKWLFGFLGVALIVFCSVFGYVSYQKHEGDVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTCKFIVSEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            3743..5374
                     /locus_tag="ACG808_000990"
                     /pseudo
     CDS             3743..5374
                     /locus_tag="ACG808_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            5901..6302
                     /gene="tnpA"
                     /locus_tag="ACG808_000991"
     CDS             5901..6302
                     /gene="tnpA"
                     /locus_tag="ACG808_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003378761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFS5046635.1"
                     /translation="MDNEYRYSKTTVSLVNYHFVFCPRYRRKIFVNSEVDARFKALVQ
                     EIAKENEWNIIAIETDKDHCHLLINVQPTYSPSKIMKIIKGKTSSIIRQEFEHLSKMP
                     SVWTRSYFVSTAGNVSSEIIKRYVENQRTRY"
     gene            6326..7444
                     /gene="tnpB"
                     /locus_tag="ACG808_000992"
     CDS             6326..7444
                     /gene="tnpB"
                     /locus_tag="ACG808_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="HFS5046636.1"
                     /translation="MKKAHKIRIYPNCRQEIQFQKTFGCVRFYWNFLLDQRITNYKKK
                     KENADYKEDKTTYASLKKMEEYAWLKEVEAQPLSQVAMDLNKAFKNTFNSTFGFPKFK
                     SKKYSKKSYRTAMGIKVNRNYFYVSKVGWVKMAETLRFNGKLMNVTISQSKTEKYFAT
                     FLVDIENFQKDPVTESIGLDLGLTHFCITSAGEKIGNERFYRSLEKRLVVEQRKLSRR
                     LEVAKKHNRKLEECKNYQKQRVKVARIHEKIFNQRNDFLHKLSSRITDENQIICIEDL
                     NVTGMVRNHKLAKSIADVSWSEFVRQLTYKCEWKGRTLVKIDRFYPSSQICSSCGHND
                     GKKELDIREWTCSNCGTNHDRDINASINIRTEGLSGIA"
     gene            7510..7875
                     /locus_tag="ACG808_000993"
                     /pseudo
     CDS             7510..7875
                     /locus_tag="ACG808_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
     gene            7888..8643
                     /locus_tag="ACG808_000994"
     CDS             7888..8643
                     /locus_tag="ACG808_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS5046637.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQKQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSEKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFAFILFLFIKKIRRRSE"
     gene            8640..9467
                     /locus_tag="ACG808_000995"
     CDS             8640..9467
                     /locus_tag="ACG808_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFS5046638.1"
                     /translation="MNKRKWLFVCVAALFATAAYSQISFADSSTTPTTSQTTDSSTTA
                     TSDTDSSKNTAEETADSSTSSTESAESTSESSSETSEKLQEVEDTQVIKQNIHIQKII
                     SSNRINNQGRQVAHQEGVNGAKFIVYDITNILEEMTQDDNEKNQSVEIIESKLKDRAK
                     KLSYDQLKKVTEGETKTIDDQEGVIDFSIEVPANKKQAYYIVNESSPENISNSEDIIL
                     LTPISDEKGEFLTDVWLYPKSKKSEPKEEIKKVVSTGVKKNFFDNCWDFFTHLFFND"
     gene            9477..9725
                     /locus_tag="ACG808_000996"
     CDS             9477..9725
                     /locus_tag="ACG808_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046639.1"
                     /translation="MKIIKMLAFNLSFLAATILCCGLVFSQLSIYPRYQRLFVHFLNI
                     QHASMPLEVINMLLINPIFMASLFLLAGLVTWVLGKKE"
     gene            9768..11867
                     /locus_tag="ACG808_000997"
     CDS             9768..11867
                     /locus_tag="ACG808_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HFS5046640.1"
                     /translation="MENKIHCTTGRVFTLVLFLFLLLFARGTAVQALEKVDPSQIREN
                     NNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAHYQKV
                     GTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQSINV
                     MNVKLDFKYSGSKTKVALSEPQPEINSKMRSMRAAGIELNDTFLSIYSLNGQYQQRVS
                     WYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSKVVGG
                     RAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTALLWTP
                     QPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKSFKIT
                     DTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSFLDNA
                     ANYGRDYILQLHMKAKNDAKGIAPNVATVTFNNDPNKGIQKTNTVENYFSLKPNKKVE
                     QDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQDPVEA
                     RLTYKEGSARVIDMSTGKDITSEGTLTYDSNLRTLKWEASADFLSKNLLDGREIQLIF
                     TAKTPLQSEKNIDNQAVAAVENVSNKTNVVTIGVDPNLPQVIVPRTGSTHLVTILVVS
                     LVLLVLVTLSYVALKFN"
     gene            complement(11903..13954)
                     /locus_tag="ACG808_000998"
     CDS             complement(11903..13954)
                     /locus_tag="ACG808_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFS5046641.1"
                     /translation="MKLLNPKTVLAIGTFAATLGFAGSADAASTNVMYRLYNPNNHEH
                     FYTSSTKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNANKVLNDSKAT
                     QKQVDSAKNNLDSAYKGLKKKPAPTVNKAELQALYNRVKSTAKGDFTEESWNDFQTAL
                     SNAKKVLDDSKASQAQVNNAKNSLDAAFKGLQHKPETPYMVTINHIDNQTGQVIATDQ
                     AQVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSQTITGNTTITFNYNQVHQINL
                     LCNNNYKNEGRVDILKEQIINVTHGESVTLTAPAIQGYVLDDRVEPTNSVTLNNVTES
                     QNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFSTPWKNMTDQNYFLCRNDD
                     PSISVDKNGQRVINNVDQNRTITFKYKNISLSDLNNYVRQKELVWLNNYRQQNGAAPM
                     QFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENINSFYLYVDEI
                     LSDTGAVNAMIMWKDSAAHNANLLYNNQSIVGLSHAYEVSADGSLSSDNVFISANPIF
                     K"
     gene            complement(14074..14283)
                     /locus_tag="ACG808_000999"
     CDS             complement(14074..14283)
                     /locus_tag="ACG808_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046642.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAIKLNKLAEETIDTNASTK"
     gene            14471..14929
                     /locus_tag="ACG808_001000"
     CDS             14471..14929
                     /locus_tag="ACG808_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046643.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLVNNLKIIDDTTWEYSESVWHPEQVQITVEKLKDYKGLERYKVIDSGGIDLFI
                     GKTALIVPYNKDGLETFKFIPTNEKEHRDKSEILKEYSTKSGYKLQKTSE"
     gene            14926..15168
                     /locus_tag="ACG808_001001"
     CDS             14926..15168
                     /locus_tag="ACG808_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HFS5046644.1"
                     /translation="MIASLFIYPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            15186..15488
                     /locus_tag="ACG808_001002"
     CDS             15186..15488
                     /locus_tag="ACG808_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HFS5046645.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAGVAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILGAASIITSLFGLFGQTPQGL"
     gene            15558..17621
                     /locus_tag="ACG808_001003"
     CDS             15558..17621
                     /locus_tag="ACG808_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046646.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHESRSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEKENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNEIEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHEIAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVE
                     KINNFTQTNQEALSKEKENLLKERIRERKEEFYQGLRTDKLKISKELNEQLNKLYQIT
                     SEALMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLELKKREQD
                     LAEQQMAIEKQRLNQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPTPSVAAVQTPM
                     NNQTNLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEE
                     AQEQARKEYEQQLEELLNRTNQATTESSMENSKESDPSKAKEGSSDSTVQSNTSESSE
                     SNEVK"
     gene            17621..20230
                     /locus_tag="ACG808_001004"
     CDS             17621..20230
                     /locus_tag="ACG808_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFS5046647.1"
                     /translation="MRVRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAILMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGGFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYQDPENDYRQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNIL
                     STAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHQGKK
                     FHEERVKVGYRGFCEINFDCHLEQGDHVCLKYDHQGKEYVAWYEFSQRVLKDEEGNIV
                     YRKKRGETHLPELEKEAVLTLDEDKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHII
                     VSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNMLW
                     DLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENST
                     TNSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNHG
                     ETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDIYARRLYHNMH
                     DQIEEEAKENIFIKIVQSLDNSTAQKLLIQFYEEKQIDRLQEYLEKLHQNEEITEVIR
                     KRLLLEAEKAVKE"
     gene            20227..22623
                     /locus_tag="ACG808_001005"
     CDS             20227..22623
                     /locus_tag="ACG808_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFS5046648.1"
                     /translation="MIIGGIFSGIANWWSGLFGDGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKIKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLETDPNKVESLPTDKRNALTAKNFKTANINSVII
                     PKEAAKMADEDKSGLSDIQKDRLRALQYRLELDDNGKEIPVEISDEGLAKYVYTSGYR
                     RYISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSNLNDIRKNGF
                     GNRKDVIPDNGEEQPDDKATIVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTSEKENQEGAAIPSAKDHQDELHSNENSIGEKDSEEGQHGQLPKTDPEQY
                     RYKSNINSENEKRISDLPKRNQEVQTDEDSIDLPNDNGIEVGTEKSMKIGLPKTQNLP
                     MKDSRLNQFKENDQSFVHQSTAGTVQMKPSRLPENGNLTTVSYNLSESGETGNVSIQN
                     VVTREAAPEPIQKVHTGYKPANVAFTAPTKINVTRETKNRNNELKEQLLRDIKQDIKP
                     "
     gene            22675..23007
                     /locus_tag="ACG808_001006"
     CDS             22675..23007
                     /locus_tag="ACG808_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046649.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            23008..23601
                     /locus_tag="ACG808_001007"
     CDS             23008..23601
                     /locus_tag="ACG808_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046650.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLVTKE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVQTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            23764..25788
                     /locus_tag="ACG808_001008"
     CDS             23764..25788
                     /locus_tag="ACG808_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion system protein"
                     /protein_id="HFS5046651.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASRLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            25788..26993
                     /locus_tag="ACG808_001009"
     CDS             25788..26993
                     /locus_tag="ACG808_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS5046652.1"
                     /translation="MENQDESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNDNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGTWIDP
                     LPFLNSSKK"
     gene            27009..27653
                     /locus_tag="ACG808_001010"
     CDS             27009..27653
                     /locus_tag="ACG808_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046653.1"
                     /translation="MENKTLKIVLTAALSISLIGNIALFWQLKEEQATNTYKIEEVAK
                     NASEKLKKNFSEAQAKIDKLNQTINEKNQEIDQLKQQDGIKAKEEVTDAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAETS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            27664..28470
                     /locus_tag="ACG808_001011"
     CDS             27664..28470
                     /locus_tag="ACG808_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFS5046654.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFTILGFLILASPIYTHKKVNI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQGLLKELANLDYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIVPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDDNYEQHAINYKVKGYKKEQKAAQKQIESLQATIDLNEELVNKLKDQLP
                     YQVADDQENTENKINDYQQEIETSKTQMKDQEEIIQKIQEKIDRMTKENI"
     gene            28493..28723
                     /locus_tag="ACG808_001012"
     CDS             28493..28723
                     /locus_tag="ACG808_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046655.1"
                     /translation="MKGANLVGKDIFAEQKVLIKAYLKVVKAKEICQEVNLSNTTISE
                     LKNGRKSLDTLKIRSFEALYLAAKKYFTAERK"
BASE COUNT        10350 a         4453 c         5866 g         8073 t
ORIGIN      
        1 cttttttttg agttataata atacgcgaag agagataatt tcattattta aacaagctaa
       61 aaatcttgat attacttctt ttcaggcaat acgaatatta ttcgcttaca ttttttgtat
      121 aaatttaatt gtctgctatg cttgtgatga attgattttc gttctgcact tttggatgat
      181 aaatttggac aattgaaaat acaaaaaaaa aagaaaggcg gtatgaactc ggaaggctgg
      241 taaccttcgt cagcgttcat acacggcatt aagatttggc ccttaatgcg ttaacctttc
      301 agtgttacgg tattacccgc aatggattta gtataacaga tattcaaaag tgataccact
      361 ttttttggct gtgttaaggt taaaaatacg tgatattacg atgaatctat gcctaagcat
      421 aatgttataa aaatgtccgt gtggctcttt gccgtatgac gaacgctgaa attagaggga
      481 acgcatgttt ggatgcgttc cctctttttt gtttgtcatg aacgaaaatc aattctctat
      541 ttttgaaaaa taacaagaaa aacttgttaa aaatatttaa agaaattggg tggtttattt
      601 tatgtcatat acaggaattt tgagtctcaa agatatttgt cactacggta aacggtgtac
      661 cgctacagag aaaatcacga agaaactatc aactggccaa agtaaaaccg ttgtgcagtg
      721 taagaaatat ataatccaga aagacaaagt ctcagaggaa atgatttatt acattgggaa
      781 acaaaagcaa ataattttaa aagatcctat ccccttaaaa gagctatatc caacaatcaa
      841 acatgtttat gaccaaaacg gtgtactgat tggtcgaaga aaaaatggag tcttgcgttg
      901 taccgcaaaa gggatggggc gtttgatcgg ctgaattgat cattctcata acttgtattt
      961 tatcgttttt cgcttgcttg aaaatattgt cgagacttct aaaggaactt agagtcacta
     1021 gaaaaataag gagtagactt gatctatcaa caattccctt cggggagtga cggggtaggt
     1081 tttaacgatt ggtaacgtaa aaaattggtc aatcgagtaa atgcgcttgt ttggatagtg
     1141 gtgaaatgaa tttgtgtaaa taatatacac agagtaaaca agagggaatt tagaagttag
     1201 acaagttaga atagcgtcta gattaatcat gagcagctac taagggatct agactttgac
     1261 gcaaactcat ggaataagga gtaggtcttc tcgtaatggg gaggacgcac aagaaattgt
     1321 gtcgtgtgct gcagtacatg tagatacttg cgaggacgtt gattattgaa tcaatcgagc
     1381 atttaccgct gtctactagg gtttctcgtt tcggacgcca taaacgagtc gaaatttttt
     1441 atgtattttt aagtaaacga aaaacgataa aatatgaaca agtcctgaat aaagtgaaaa
     1501 aattgatata aaacatagag aaaatgaaat ttacgtaatg gaggaatgac aaatgaaaaa
     1561 ttatgatccg aatatacgat tgggcacaca tactattaag gtttctttcc aacgttggga
     1621 ttacaaagga tttgtgacgt ttagaagggg cggaaattgt aaaggattag atgttttagc
     1681 attagacgag gatgatttat acgatcaaac attgactgat aatccaattg gattcggctt
     1741 gctacctgaa gatgatgaag gtaatgaatg gtttaaaatg acgcttatga atgataatgg
     1801 agacgaatta tctgttgaag atacttggtc atatttgagt gattatattg tcagcgttga
     1861 aatcattgaa tttgtagcgg ataaagaaga ataaaaactg gaggaataaa ttatgaaatt
     1921 tttcgaagta aaatcaccat actattatgc gttaatcgca gcaaagaatg aagaagaatg
     1981 tgcagaattg tatgaacaag tagtaacaga tatcgaaaat cgtgaagaat ttattcgatg
     2041 tttgcatgag ttaaatcgaa atgaagcaat tcaagaggat gcgaaaaaaa ttagtgaaga
     2101 aacgttacaa ccaatcggta tggaggaagc tacaaaagac atttttacaa ttattgatga
     2161 acaaaaatct tgtgtattaa gcattgattc tacattggtt taatagatcc aagattctat
     2221 aatttttttc aaagtaaaaa gaaattaggt aatgattctc agaccaatta cttaggaaaa
     2281 taaccataca aattagattt tacagataaa ggattagaag gaatcacggc gattaataaa
     2341 aaggtagtat taccttaggc attgagtaag gaatttattt aggaggaaaa ttagatggcg
     2401 cgaccagttt tagatgcgat ggatatggat actttgacaa aaggaaggta tgtacagctg
     2461 ttagctcgtg gttattcacc taaagatgta ttcaaagccc tatctaaagg aacagatatg
     2521 gaaaaagaaa gattacgaaa agaattcgac tattggagag agcataacgg aataaaagat
     2581 ttaaaaccag cacgtccagt tattagacga aaaaaattga agaagtaaga ccaaagcgta
     2641 tggcgtacat aggctttttt attttgataa aaataatgaa aggaaggaaa accatgaatt
     2701 gtaaagaaat cgaaaataga aaaaaagtgt cgaaagaaat ggaagaaaag ttactaaaaa
     2761 caatgaaaca aaaacacctt aaaagattat ctgtcgcgca atatatcaat gatatgcagt
     2821 taacaggaaa agagaaagct tgtttgttag gttcaatgaa aaactttgaa cagttgagac
     2881 gaacttatgt gaaaaaaagt tctaattgtc aactattgtt agaagtctca tgacattcat
     2941 taatagaatt catttttaaa actttttcta tggaaatgag aaaaagtttt tttgttttgt
     3001 ctgaaaggag gcagatggat gaaaaaatgg ttatttggtt ttttaggagt cgctttaatc
     3061 gtcttttgca gtgtgtttgg ctatgttagc taccaaaaac atgaaggaga tgtgtttaag
     3121 caaaatatag aaaaaaagat gcccgtagat caaatcaatg cacacgcaaa gagttataag
     3181 gaggatgcaa cgaacgtcaa taatgatatg tctttggggc aaatgttgtc catacaaaaa
     3241 gaagcaatcg aaatgggcgt caataaacaa gtatttgcac aaattcagat tcctgcactt
     3301 ggtttagcat tgccgatttt taaaggggca aatcaataca ctttgagtct tggagcggct
     3361 acttattttt atgaggatgc cgaaatgggc aaaggaaact atgttctagc aggacacaac
     3421 atggaaatgc caggtgtttt attttcagat attcaaaagt tgtcattagg ggaggtgatg
     3481 gacttagtta gtaacgatgg cgtctatcgt tacaaggtaa cgtgtaaatt tatcgtatct
     3541 gagtacttta aattaattga tggcgttcct gaagagaact ctttcttgtc tttaccaaag
     3601 aaaggagaga aacccttatt aacgttgttt acttgtgttt atacgtcaca aggaaaagaa
     3661 agatacgtgg tacagggaga gctacaataa caaggttgta atcaacgcga gtcgttgaaa
     3721 tatgaaatga ggagagtaaa aaatggcaat aaatcggtat gtaaaaaaag ctggaatgct
     3781 tgttggaatg ttaggaatca ttgcaggctg ctttcaggga tatcgagcat atgcagaaga
     3841 tgtcacgcaa aaaacgccac cagagaaggt caatatcacg gttcataaat taatgtacga
     3901 tcaaggaacg caattgaatg ttgatattga cgggatcaaa aatgatggtt atacgcacga
     3961 tcaatatcct acaggtgtaa cgaaatacaa caaggcagat tatggtgatg ttgagtttac
     4021 gttaggaaat attacggatc aagtattacc gagggaagat tctgatctaa ccaacgcgaa
     4081 agtggacgaa atcgttaaag atgtagaaga cgagggatct aacagtgagt acgttaagaa
     4141 tgcaaccaat aaaattactt cagctgtaga tgaaaatggt gagatcactt ttgctgatca
     4201 accagcctat gtcaattcca aagggaatgt atatgtggtt tatgaaagta aatccgctgc
     4261 tggattagtc acacaaaaag caaaaccaat ggttgtgatt gcgccaatga ctgataatac
     4321 aggttcaggt tttttaaaag acattcactt atatcctaaa aatattgttt ctaagttgag
     4381 ttttgaatta acgaaatttg gtgacgatgg cacagctcaa agcaaacaaa cacctttaaa
     4441 aggggcaaaa tttgagttat acaagggcga acctggaaaa ggaacaaaat taggagatct
     4501 tgtatcggat gatcaaggaa agctgactgc tactgatttg acgttaggaa aatactattt
     4561 tgtggaagtt ccttcagaag ttgtagtggg atcagataaa gaaccaacag cagatcaata
     4621 tttattaggc gcagacgctc gcaacgatgc gcacaataaa ttaacctttg aaattacgaa
     4681 tgatggcgtg acaagtgact taaaagcttc atacgtaaac tataaagcac cagtgatcga
     4741 taaaacagta acaaatggaa caggacaaga acattctttc caaattggtg acgcagtaaa
     4801 ttatcaagga acgattcata tcccaacaga cattgcaggc ggagcagacg gtatcacagt
     4861 aaacggagtg aaaagcgaaa cttctcctta cagtgtattt aaatgggggg atacagcagg
     4921 acaaggactt tcttatgtcg ctgaaaaagc aaatatcaaa gtcacaaata atgatggttc
     4981 agtcgtatta aaagaaaata ctgactataa aatcgaaaat tctgactata aaatcgaaaa
     5041 ttctgatagt ggttttgtga tcgattttat cgtaaataat ggacaagtaa gtgatacagt
     5101 tgcgaattta catggtcaag atctaaaaat gacttataac atgtttgtga atgatagtgc
     5161 tgaaatcgca aatccattaa caaatagcgt ggatttcgtc tatagcaaca atccattcaa
     5221 tcaggaagaa caccatgaaa aaacgaaagc cgacgctgta acttatggcg caaaattctt
     5281 aaaagctgat ggcggactat ttggaacagg aatcaaagca acaccgcttg aaggcgcaga
     5341 atttgctgct aaaaacgcag aaggaaaata ttatagcttt tgcgggcgat aaaacccacg
     5401 gttttaaccg tgtggatgat agcccgcact gtactgtaag agcataggga aacttgtgct
     5461 tagtggaata tataaaatct cgtatatccc aacaaagaaa ctgaattgcg gggaacccct
     5521 aaagcgagta aaacgacaac gtagtcttga aataggcaaa cgtgaccgtg gcgaaagcag
     5581 aaaaaattta ctcgatagca tatggttaaa tcctaagtgt tgcgacaatg ggcaatctgc
     5641 agcgaagact cgaacagagt aacgttcaac gactattcct cttgagggaa gtacacacaa
     5701 gcgtgtggaa gtggtttcgc cgtactgggt aaagccaacg gataagatat agtctgtgct
     5761 ggcacgaaag tgtcagaagt tcatcagaga actgcatagg aagtagcgaa cctatgtgaa
     5821 caaactccac ataaaaaatt cttcattctt ttgtgtatat taacattgat ttagtacatg
     5881 gcttaaccta aaattaaggt atggataacg aatacagata ctcaaagaca actgtatcgt
     5941 tagtcaatta ccactttgtt ttttgcccaa ggtatcgtcg gaaaatattc gttaattctg
     6001 aagtagatgc tagatttaaa gcactcgttc aagaaatagc aaaggaaaac gagtggaaca
     6061 tcattgcaat agaaacagat aaagatcatt gtcatttatt aataaatgtt cagcctacgt
     6121 attctccttc taaaatcatg aagataataa aaggtaagac atctagcata atccggcaag
     6181 agtttgaaca cttaagtaaa atgccaagtg tttggacacg ttcatacttt gtttcaaccg
     6241 caggaaatgt atctagcgaa attatcaagc gatatgttga gaaccaaaga acaagatatt
     6301 gattgcgtta aggaggtgtg cagaaatgaa aaaggctcat aaaatcagga tttaccccaa
     6361 ctgtcggcaa gaaattcaat ttcaaaaaac attcggatgc gttcgtttct attggaactt
     6421 tctactagat caacgaataa cgaattacaa gaagaaaaaa gaaaatgccg attacaaaga
     6481 agataagaca acgtatgctt cattgaaaaa aatggaagaa tacgcttggc taaaagaagt
     6541 tgaagctcaa ccacttagcc aagtagccat ggatctaaat aaagcattta aaaatacgtt
     6601 caactctact tttggatttc ctaaattcaa aagtaagaag tattccaaaa agtcctatcg
     6661 aacagctatg ggtataaagg tgaatagaaa ctacttttat gtatccaagg ttggctgggt
     6721 aaaaatggct gaaacgttgc gtttcaatgg taaattaatg aatgtgacaa tcagccagtc
     6781 aaaaacagaa aaatattttg caactttttt agtcgatatt gaaaacttcc aaaaagatcc
     6841 tgtgacggaa tccattggtt tagatttggg tcttactcat ttctgcataa cttccgcagg
     6901 tgagaagata ggaaacgaga gattctatcg atcattagaa aaacgcttag tggtagagca
     6961 acgtaaactt tcaagacgct tagaagtagc aaagaagcat aatcgtaaat tagaagagtg
     7021 caaaaactac caaaagcaaa gagtgaaagt ggcacgtatc catgaaaaga tttttaacca
     7081 acgaaatgac tttttacaca aactgtcttc tcgtataact gatgagaacc aaatcatttg
     7141 tattgaggac ttgaatgtaa caggcatggt tagaaatcat aaattagcta aatcaatagc
     7201 agacgtatct tggagtgaat ttgtaaggca attaacatac aagtgtgaat ggaaaggtcg
     7261 aacgttagta aaaatcgacc gtttttaccc tagctcgcaa atatgttctt cttgtggtca
     7321 taatgacggt aagaaagagt tagatattag agaatggact tgtagcaatt gcggaacaaa
     7381 tcatgacaga gatatcaacg ctagtataaa cattagaacc gaaggacttt cggggatagc
     7441 ttagtaaatt tgcgcccgtt tgggtgtatt acctaagaac cccatgactt tagtcgtgtg
     7501 gagtgtcagg gtggattagt tgatacggat aaagacggtg tcaaagaagc agtatgggta
     7561 gacaatgtag caaatgcagc gatcctaaaa tcagataaag aagggcgctt tgaaatcact
     7621 ggtttaactg aaggtgaata ttcattggaa gaaacaaaag caccaactgg ttaccaaaaa
     7681 ttaacagaag atattgcatt taaagtaaac aaaaactcat ttaaagagga aaatcgaatc
     7741 actgttaaaa ataatcaaaa agcagctatt ccattaacag gtagtaatgg tttccaaacg
     7801 tatgtattga tctcttgctt gttgctagga gcaggggcac tttctgcagt tgtttacttt
     7861 aagaagaaag cctagaagga tcagctaatg attattcgta agcccccaaa aagaagagtc
     7921 atgcacaaac tgatgatatt tttttggttg ctgtcggcag tagcagtcta tttaattcca
     7981 gttgtggaaa caaatgtagc taaatttcaa caaaagcaaa gaattttagc cattgaaaat
     8041 cttgaaaaaa cagattcttc tttggatgtt gcgaaaatag agttaggcga tcctgttggt
     8101 gttcttacaa ttccaagtat ttctttaaaa ctaccaattt atgacggaac ttccgaaaaa
     8161 attttagaga atggtgtagg gattacggaa ggaactggcg atattacagg aggaaacggt
     8221 aaaaatcctt taatcgcagg gcacagtgga ctttataaag ataatctttt cgatgatttg
     8281 ccttcagtaa aaaaagggca aaaattcttt attaaggtaa atgaagaaca acatgcctat
     8341 caaatttatc gaattgaaga agttaaagca gaagaattac aaaagaatta tttagattat
     8401 ctcatgcctg aaaaagacca agataggatt acgttaatga cctgcacgcc aaaaggtgtc
     8461 aactcacacc gctttttagt gtatggtgaa cgagtttcat tcagtaaaga agatttaaaa
     8521 ctacagaaaa aagcaaaaaa taggaaatca atttcattgg attggcgatt agcactgcta
     8581 ttcgcttttt ttgcgttcat attattttta tttataaaaa aaataaggag gagatcggaa
     8641 tgaataagag aaaatggctg tttgtttgtg tagcagctct ctttgcaact gcagcttact
     8701 cacagatatc ttttgctgat agcagtacca ctccaactac cagtcagaca acagatagtt
     8761 caacaactgc aacaagcgac acggacagtt cgaagaacac tgcagaagaa acggcagatt
     8821 caagtacgag ttctactgaa tcagcggaat caacgtcaga aagttcttca gagacttcag
     8881 aaaaactgca agaagtagaa gatacgcaag taatcaagca aaacattcat attcaaaaaa
     8941 ttatttcttc gaatcgaatc aacaatcaag gaaggcaagt agctcatcaa gaaggtgtca
     9001 atggcgcaaa gtttattgtc tacgatatca cgaatatctt agaagaaatg acacaagatg
     9061 ataatgagaa aaatcaatcg gtagaaataa ttgagtcaaa attgaaagat cgagcaaaaa
     9121 aactttcata cgaccagttg aaaaaagtga ctgaaggaga aaccaaaaca attgatgatc
     9181 aggaaggtgt gattgatttt tcaattgaag tacctgcaaa caaaaaacaa gcatactata
     9241 tcgtcaatga atcaagccct gaaaacattt caaatagcga agatattatt ttattgacac
     9301 ctatttcaga tgaaaagggt gaatttttga cggatgtttg gttatatcct aaatcaaaga
     9361 aatcagagcc gaaagaagaa attaaaaaag ttgtatcaac tggtgtaaag aaaaactttt
     9421 ttgataattg ttgggatttt ttcactcatt tattttttaa cgattaggat gaaatgatga
     9481 aaataataaa aatgttagct tttaatctat cttttttagc cgcaacaata ttgtgttgcg
     9541 gtttagtttt ttctcaatta tcaatttatc caagatatca acgattgttc gttcattttc
     9601 taaatatcca gcatgcttcc atgccactag aagtgattaa catgctctta atcaatccaa
     9661 tttttatggc tagtttgttt cttcttgcag gattggtaac gtgggtatta ggaaaaaaag
     9721 aatgagaagc ttacaatcgt ttaacaaaat aatagatgga gggcattatg gaaaataaaa
     9781 ttcattgcac tacgggaaga gtatttactt tagtgttgtt tttgttcctt cttctttttg
     9841 caaggggaac tgcagtccag gccttagaaa aagttgatcc gagtcaaatc agagaaaata
     9901 ataatctggt actttatcat gatagtgatt cattgagaaa tatcgaagta actgggatca
     9961 atggatttaa gatactagat gagtattcaa ctaaaaaaca aaaagtactt gcttggccaa
    10021 aagtcaaaga tttgaaaagc acgtcaaaaa ttgttgccca ttatcaaaag gtgggcactt
    10081 atctaggaaa agaaattgat tgcgacgtaa ctttttctaa ttttgtagcg cacgctgggg
    10141 ttaaccaaga cggattagct aaaacaatta atgggaaaaa gtattataat tatttgctct
    10201 ttgataaaaa catttatcga ggctttttca tgcagtcaat caacgttatg aatgtaaaac
    10261 tagattttaa gtattcagga tcaaaaacaa aagtagcttt gagtgaacca cagccagaaa
    10321 ttaattcaaa aatgagatcg atgagagcag ctggtattga gttgaatgat acatttctat
    10381 ctatttacag tttaaatgga cagtatcagc aacgtgtgtc ttggtataat gacaataatg
    10441 aatctgtcgg tgaacgtaat attgatatga gagaatttgt tgggtatgaa aaaatgggta
    10501 gcttacctta ttttgtcaca acagatacag catgtgcaga atacaaagct cctgcgttat
    10561 caacaaacaa tttaacttca aaagtagtgg gaggacgtgc agaaaaggct tatagctcga
    10621 atgatcattt caccgatgtt gtaggagctg atacttatca cagaagtggt gtaacgtata
    10681 cgcttcaagg cgcttcccca acattcatga ttggcgcaaa tacgaatagt atgatgttta
    10741 gctttgatac tgcattgcta tggacaccac aaccatcgaa gcctacaaaa gaagtgttta
    10801 acaaagctaa tactgaagag gcagcacaca atattgacaa aaaagtgatt ccacaaggat
    10861 cagatgttta ctatcatatt caccaaaagt ttgatgcatt aacagtcaac acaatgaaca
    10921 aatacaaatc atttaaaatc actgatacct ttgacagcaa aaattttgat atggtatcgg
    10981 atgggaaaaa ctatgatggc gcattgtggt atgttgaagc gaatgggact tggaaaaaag
    11041 ctgatacagg aggaaaatta actgtatcag gtaatacagt cacttatact gcttctgaca
    11101 gctttttaga taacgctgca aattacggta gggattatat tttgcagctc catatgaaag
    11161 caaaaaatga tgcaaaagga attgcaccaa atgtggctac tgtaacgttt aacaatgatc
    11221 cgaacaaagg aatacaaaaa acaaatacag tagaaaacta tttctctctt aaaccaaata
    11281 aaaaagtgga acaagatggg aaagatgttg atggtcgaaa tgatggtaaa aaaggaacgc
    11341 ctacagctcc tttaaatgca ggatctgaag tacaatatct agtcacacaa aaatggcata
    11401 caaaaggtgt agacattgcg tcagatcatt acaagcaatt tagtattcaa gatccagtgg
    11461 aagctcgttt gacgtataaa gaaggttctg cgcgagtgat agatatgagt acaggaaaag
    11521 atattacttc agaaggtaca ctaacctatg atagcaattt aagaacgctg aaatgggaag
    11581 cttccgctga tttcttaagt aaaaatcttt tagatggacg agaaattcaa ctgatattta
    11641 cagctaaaac tccactacag tcagaaaaaa atattgataa ccaagccgta gcagcagtag
    11701 agaatgtttc taataaaacg aatgttgtaa caattggtgt tgatcctaac ttgccacaag
    11761 tcattgttcc tagaacaggt tctacacatt tagtaacaat tttagtggtt agcttagtat
    11821 tacttgtgtt agttactctt agttatgtgg ctctgaagtt caattaaaca aaaaaacaag
    11881 gctgaaaatc agccttgttt ttctatttga aaattggatt ggcactaata aatacgttgt
    11941 ctgaagaaag tgatccatct gcagaaactt cataagcatg agataaccca acgatgcttt
    12001 gattgttgta aagtaagttt gcgttatgag cagcgctatc tttccacata atcatggcat
    12061 tcactgctcc cgtatctgaa aggatctcgt caacgtaaag gtagaaacta ttgatatttt
    12121 ctcctttagc gccgtagcag cctaaacttt ctaagagatc ttggaaagta ccgccgccag
    12181 gtcgataatg actgaagctt acttgtaatt cttttgcacg tatatcagca gcttgttgaa
    12241 cgatatcatt aaattgcatt ggagctgcac cgttttgttg acgataatta tttaaccata
    12301 caagttcttt ttgacgcaca tagttgttta aatcacttaa tgagatattt ttgtatttga
    12361 acgtgatcgt tcgattttgg tcaacattat tgattactcg ttggccgttt ttatctacgc
    12421 taatggaagg atcgtcattt ctacacaaga aataattttg atcagtcata tttttccaag
    12481 gagtagagaa gctatcccct tcgtatactg tttttgattc actagagaga acagcgttgg
    12541 tatcagcatt tacatgatta acagtcacat taaatttacg agtgtagttg aaaatataat
    12601 tttgactttc agtcacattg tttaaggtta ctgaatttgt tggttctacc cgatcgtcta
    12661 aaacatatcc ttgaatagca ggagcagtta aagtgacact ttcaccgtga gtgacgttga
    12721 ttatctgttc tttcaaaata tcaactcgcc cctcattttt gtagttgtta ttgcacaaca
    12781 aattgatttg atgcacttga ttataattga aggtgattgt tgtattacct gtgatcgttt
    12841 ggctttgagt atcattacca atcactttgt agctaaaatt atctttagcg ccttcgctat
    12901 atttaaatgc ttttgcctta gcggtgtaag tagaaccaga agtcacttgt gcttgatctg
    12961 tagcaatcac ttgacctgtt tgattgtcaa tatggttaat ggtaaccata tatggcgttt
    13021 caggtttgtg ttgcaaccct ttaaatgcag catctagact atttttagca ttatttactt
    13081 gggcttgaga agccttagaa tcgtctagta cttttttagc attggataaa gcagtctgaa
    13141 aatcattcca agattcttct gtaaaatctc ctttagcagt tgattttact cggttgtata
    13201 aagcttgtaa ttcagcttta ttcacagtag gtgctggttt tttctttaat cccttgtaag
    13261 cagaatctaa attattttta gcactatcca cttgtttttg agtcgctttt gagtcattta
    13321 acactttatt agcattattt aaagcagttt gaaatgcgtt ccaggtattg tcggtatagt
    13381 cacctttttt cgttccctta acgctcgtat aaagagcttc taaagctgat ttattcactt
    13441 tgggtttaga ggtgtcagcg ccataccaac cgataccttc gtatttccag ccatagtctt
    13501 tggttaacca ttgtacttct ttccaacttg cagtgtagtg atgtgagcca gattttacac
    13561 caggaaggaa aaggcggtag atcggtttat tatttttttc ggcagaacgc caggcaggcc
    13621 cttcataagt ccagccgtaa tttttagtca accatttcac ttcatcccaa ctagctgtgt
    13681 aatggtgatc acgaagacct ttattgtata aacgatagac aagatgacca ccgctagtcg
    13741 gtgcttccca agcggggcct tcataattcc cccacttaat cttcactagg tgatcacgtt
    13801 cttttgtgct agaggtgtaa aaatgttcat ggttatttgg attataaagg cgatacatga
    13861 cgttagttga agcagcatca gcagatccag cgaaaccaag tgtggcagca aatgttccaa
    13921 tagctaaaac agtttttggg tttaataatt tcatattttc aactcctaaa aattagttaa
    13981 aatcgattac aaaatcatta tatatcggta aaccgatatg gtcaataaaa aaattaagaa
    14041 aagcctcatg tatttctggc ttttcttaat ttgttactta gttgaagcgt tagtatcaat
    14101 ggtttcttca gctaatttat ttaattttat agcattgatg aatctcatat tgtctatact
    14161 agagcgctta tttgcgagat cactgaccgt agttaaaggt acgccagatt ctatagatat
    14221 agaacgaact gatttaccac tatcaaacag caaccattcg attttctttt tatcagcgta
    14281 cattgcaatg ctccctcttg attttttctt tattatagct cttttttgta ataaatcaca
    14341 atatcaagag agaaagtttt tatagaaagg tgggaaatta tgagtatccg ttccccgtgt
    14401 atcaaaatga ggtttgttgc tactttcgtt atttgatata ctaaaataaa acaacgaaag
    14461 aagaaataaa atgaagaaaa agcaactatg gtttgtctct atgtttgcaa tattttttat
    14521 tattttaagt ggatgttcaa gtaataagaa atcggaagct aaagaagata atagtttatt
    14581 gcaaccagga actgtttgga aagaagaagt aggtggtctt gttaataatt tgaaaattat
    14641 agacgataca acatgggaat attccgaaag tgtttggcat cctgaacaag tacaaataac
    14701 agttgagaag ctaaaagact ataaaggatt agaaagatat aaggtaatcg attcaggtgg
    14761 aatagattta ttcataggta aaacagcctt aatagttccc tataacaaag acggtttaga
    14821 aacgtttaaa tttattccaa caaacgaaaa agaacatcga gataaaagtg aaatattaaa
    14881 agaatacagt actaaatctg gatacaagtt acaaaagact agcgagtgat tgctagtctt
    14941 tttatttatc ctgaaggagg aatcacagtg gcaaaagcta aaacgccatt tgaaaaagcc
    15001 gcgtctaaaa tcttagaacg tcgcggtgta tctgaaacac agtggaaaca agaagtcctt
    15061 gaaaaagcac aatttgacct attacgggga aaagacaagg acttagaagg atatatctat
    15121 caacgtgaac gtgaaaagct tgttttcgat gaaattatta aacactaaaa ggagcgaaca
    15181 attttatgtt tgaatcaatt acaatgaatt ttttaacaca accgaaagta gtagctgcgg
    15241 ttgatatttt tagagcatta caaactgcag caactagttt aaaaaacaat ttgatgattt
    15301 ttgctggtgt tgcggccgtt gcttccattg ttattggggg gcttttcttc ctatttggac
    15361 gaagaggagc tgaaaacggg aagatgcacg taggttatgt attgatgggg gtaggtgtta
    15421 ttctaggggc tgcttctatc ataacttcgt tatttggatt atttgggcaa acacctcaag
    15481 gattataaga aagtaaaaaa gtaggagtaa gaagtagtcg atacttttta ctcctttttt
    15541 atgaaaggag aatattcatg tactttacga ttgcaggcaa taccaaaaca gataaagagt
    15601 taattaaaaa agcaacgaag gaagtaggaa agcggacttc ttttttaccg aaaacaatta
    15661 aatattctgt cattgaaacg gaagaacagc tacatgtgtt agggaaagta ctggaacacg
    15721 aaagtcgatc ttctgttaag ttgactctca cgttacatga cacagaagga aaaatagaga
    15781 aagctggtac agaatacgtt tttggcccgc tggattatca aatttcagaa ggtttctatc
    15841 ctacattgat ggatttaatc cgccttgatg tgtttaaaga aacggatgaa actatgagtg
    15901 tggaagagtc ttatcgaaac gaaaaacttt tacaagtttt agaagaagag ttagagagcg
    15961 accaaagaaa agtgaatcaa gtgagtgaag tcgaagtaaa tgaagaagta ccatgggaac
    16021 gaccaatctt tccaaagaag gagtcaaaag aaaaagagac agtctatgtt gacgaacaag
    16081 ctgatattcc gactcagaaa tcgatatttg agaaagagaa cgatgtgatg ggtcagttgg
    16141 aggtagatcc ctttgaagaa acaatggagg atgaagagac ggccgaagta gaagttgatc
    16201 cagttgaagc taatgaagag tcaacatgga ctgaagggga ttctcaggaa tatattgaac
    16261 cacttatcag tgaagaagta gaaaaaaacg aaatagagat acaaaaggca actgtatttg
    16321 attatatttt ggatcaatat ccgccagagc atgaatggat gaaagaaaaa aacaaattat
    16381 ttttaaatga gctttattcg aaatatcaat taccccaaac ctatcaagca tttttagaga
    16441 gtaaagaaga attaatctca cagggtaaag gcacactttt agaacagtta gagtctgtcc
    16501 aatcaacaaa ttggcatgag attgcacaag aagatttatt agccaccttt gaagaacgtc
    16561 aacaagaaac agatcaagaa attaatgagt atgcgacgga acagtcgtct aattgggaga
    16621 aagcaaaaga ggaactaaat aaagaagaac agcgagtgat tgaagaagaa gtacaaaaaa
    16681 ttaaagagcg atacgaaaag aaacgcgaca atgcgtattc cttgtgtgta gaaaagatca
    16741 ataattttac tcagacgaat caagaagcgt tatcgaaaga aaaagaaaac ttgttaaaag
    16801 aacgtataag agaacgaaaa gaagaatttt atcaaggttt gcgtacagat aagctaaaga
    16861 ttagcaaaga attaaatgag caactgaata agctctatca aataacaagt gaagctctta
    16921 tggaaaaaca cgagcagatc cagcaagaat tacaagtaca aattccaaaa tggaaaaaag
    16981 agtatgaaat tcaacaacaa gaattgaaag aacaagaaga aaaacaacgg caaaaagaaa
    17041 aagagcgcga acaattagaa ctaaaaaaac gtgaacagga tcttgcggaa caacaaatgg
    17101 caatagaaaa acaacggtta aaccaagaaa aggaaaaaga agcagctgct cgtaaggaac
    17161 gtcaagaaca aatggataca ttacgacaag ctcagttaaa ttatctagca caacctcaac
    17221 caacgccttc agtagctgct gttcaaaccc caatgaacaa ccaaacaaat ctaactcctt
    17281 caccacagcg ttcctttaac ggatggatgg tgggatgtat tgcaagttta agcttgcttc
    17341 taggtggagg gacagtattt gcttttaatc atatcaatca atcttcacag gctgattctg
    17401 tagccacaac ggcgtcaatc aaggaagaag cccaagaaca agcaagaaaa gagtatgaac
    17461 aacaattgga agaattattg aatcgtacca atcaggcaac aacagagtca agtatggaaa
    17521 atagcaaaga gagtgatcct agtaaggcta aagaaggatc aagcgattca actgtacaat
    17581 caaacacttc cgaaagttca gaatcaaatg aggtgaaata atgcgtgtta gagaaacaac
    17641 gagacgaaaa gacaaacgat tcaatttaga aaagaatagg tatctttctc atttctatcg
    17701 ctatactcct tcaggagaag agctaccaga aaaacatgac aaacgtattt ctcaaaagat
    17761 tttttatagt ggcttagctt atcttccttt aaactatgtt gttacaagaa taccagaagc
    17821 atttcatcga tttgagatta ttccaggaag tttacaagga gtgaatattt ttcagttaca
    17881 agaaatgccg cagttattcc attttgattc aactggtttt tggaatcctt tgaccccaac
    17941 aacaagttta ggcacacgtt tagttgtttc tacgatttgt gttgttccag ctatacttat
    18001 gggattgaaa atggacatgt ggtttcgtcc tctagcagat gggcaaaaag gagataaccg
    18061 attaaaaacg ttaaaggaaa tccaacaaat ttatccgcaa ataccagaat caaagggagg
    18121 ttttcctggt tatggcggaa taccaattac tcattataaa aagtactggt atattcagac
    18181 ggatacagta aatacttgta ttgttggtac ttcccgttca gggaaaggac agatagaagt
    18241 tttagcaacc attgacaatt tgtctcgtgc agaaagacaa agttcaatgg ttgttaatga
    18301 tccaaaaaca gaattatatg ttgctagtaa agatgaacta gaagctagag ggtatgatgt
    18361 gtatgcattt aatatcaccg atccactgca atcaatgagt gataatccat tggcactcat
    18421 cgtgaaatac tggaagcgag gagacataga tactgcaacg cagttaacca atacttttac
    18481 gaatacgatt tattatgatg ccaatgcttc agacaacaaa tactttaacg aaaatgcgca
    18541 gaaagctgta aatgcgctta tttttgcgtt gttagaacaa gccgatcaaa caggggatta
    18601 ctccaaatta acacccaata atcttgttga gcttctttct gaaattggcg gttttaatta
    18661 tcaagatcct gagaatgatt acagacaact caatgcctta gatgaattca tgaatcagtt
    18721 accacctgga aacatagcaa aaaaacaata tggggcaaca aaaattggtt ctgataaagc
    18781 aaaagggaat attttatcga cggcaatcac aggattgaat ccatttaccc taacgaaaat
    18841 tgcgaaaatg acttctcaat caaccattga ttataagtcc attggttttc cgaagtattt
    18901 ggatctcaaa ctacacgaga gtctgttaaa tcagcgaatt gttgtagagt tttatcatca
    18961 agggaaaaaa tttcatgagg aacgtgtcaa agtaggttat agaggttttt gtgaaatcaa
    19021 ctttgattgt catttagagc agggagatca tgtttgctta aaatacgatc atcaaggaaa
    19081 agaatatgtt gcttggtatg agttttctca aagagtattg aaagacgaag agggaaatat
    19141 cgtttatcga aaaaaacgag gagaaaccca tttaccagaa ttagaaaaag aagctgttct
    19201 aacattagat gaggataaaa gtaacttatt agtagagtct attcggatgc actataccga
    19261 taaaccaatg gctattttta tgctcacacc tgattatgat ccatctaacc atatcattgt
    19321 tagtattttt cttagtcagt tgtataaaga attatctacg caatgtgtta agactaaagg
    19381 agataaatgt caccgtagga ttcattttat tttagatgaa tttgggaata tgccaccaat
    19441 ggataatatg gaaggaatca tgacagtcac agctggtaga aacatgttgt gggatctgtt
    19501 tattcaatcc tatcagcaac tttatgcgaa gtatggggaa caaaacgcag cgattatcaa
    19561 agaaaattgt cagacacata tttatattat gtcgacagac gataatacca tcgaagaatt
    19621 ttccaaaaaa gtggggaata agacggttga acaagagaac agtaccacca attcattagg
    19681 tatgaatact catatcaatc gcaatgttga ttcagatcgt atcttaactc ctgaaagaat
    19741 ttctactttt ttagaaggtg aaacgattgt actcgctcct ttaaaacgac aagatttgaa
    19801 aggtcataaa atacggccgt atcctatttt taatcatggg gaaacgaatc ttccttatgc
    19861 ttatcaatat attgaagatt tcaatccatc gtgggatatc aatgatatag acatacattg
    19921 tacacatgcg gacttggatc ttaaggcttt acaaattgat tacgattatt ttctacctga
    19981 tatctatgca cgtcgacttt atcacaatat gcacgatcaa atagaagagg aagctaaaga
    20041 aaacattttt attaaaatcg ttcaatcttt agacaactca acggcacaaa agttacttat
    20101 tcaattttat gaagagaagc aaatagatcg tttgcaagaa taccttgaaa aattgcatca
    20161 aaatgaagaa attacagagg ttataaggaa acgacttttg ctagaagctg aaaaggcggt
    20221 gaaagaatga tcattggagg aatattttcc ggcattgcca actggtggtc aggcttattt
    20281 ggggatgggg atgcagatcc caaaaccgta acgaaagtct tacaagatta tggtaattat
    20341 ctgcagtatc aagatgtatt cagttggatt tgtaatactt ttttatgggg tttgattaaa
    20401 gggttatata atctcaatac tttactagaa aaaacaattt atcaaagctt tgatttaaaa
    20461 gacttattga acgctgctgg tgttaatgaa ctgtttcaaa gtttgattag taaggtattg
    20521 gctctttttt gtgtcgtcac attaatttat ttaggcttaa aattttttat gagtaaacat
    20581 ccaccaaaaa tcaaaaatat cttggttaat ctatttatgg ccatgatttt gatttttggt
    20641 ggtagttctt taatcgatga agggctaaat attagtaaaa acttttacgg agacgtgact
    20701 gcagcaaata aagcagataa aactggatca cctgcttttc aaattattca aaataatgtg
    20761 atcgatgtga gtacgctact agaaactgat ccgaataaag tagaatccct accaacagat
    20821 aaaagaaatg ctctcacggc gaaaaacttt aagacagcaa atattaattc agtgattatt
    20881 cctaaggaag ccgctaagat ggcggatgaa gataagagcg gtctatcaga tatacaaaag
    20941 gatcggctaa gagcgttaca ataccgactg gaactggatg ataatggaaa agaaatccct
    21001 gtagagatta gtgatgaggg gttagcaaaa tatgtgtata cttcaggcta tcgccgatac
    21061 attagtagtc cagggattat tttagctggt gaatcttctt tgtcggttgc ctatttattt
    21121 atcttgttta cgattgtaac ttgcattatt gagctggtat ttaaaaaatt ctatttagta
    21181 attgccgcgt caacggactt tgagacagga caacggatga aaagtgctat agaagatatt
    21241 tctcaatcct tcttgttatt ggcgtttacg attttagaat tacgaatcta tacattaatg
    21301 ctgtcaggta tcggtgattt gcacgcagca ggcaagatca acggattttt gtatgttgtc
    21361 gctttaattg tactaaccat tgcgctattc aaaggctctc aatctgttac gaagattttc
    21421 ggtgttgata caagtctgaa aaacggcagc aattctcttc ttagcatgtt tgccttagga
    21481 aatattgcga aaaatgtggg tagtggggct aaaaaccttg caagtgctgg taagagtggt
    21541 ttaagcaatt taaatgatat tcgtaaaaat ggatttggca atcgaaaaga tgttattcct
    21601 gataacgggg aagaacaacc agacgataaa gctacaattg tacctaatag taagaaaaca
    21661 gtttccgaaa tgttcgggaa agcgaaaaat ggtgtttcta aagccgcaga aggtcttggt
    21721 tacgtcaatg aacgtgggtt aaaaggaaca gctgaagatc ttacagaaaa agccaaagac
    21781 accttagaag agaaattaga cggcacgaga gctaaagaat tggctgaagc tgtgaagaat
    21841 ccatccgaag cactagaaaa acaaaaagaa aaagcttcag aagtcaaaga aggactaaaa
    21901 gatgcgtatg gtgaaggaca agtcaatgca gcagtgaagg caaatacaac aagtgaaaaa
    21961 gaaaatcaag aaggtgcagc gattccttca gccaaagatc accaagatga gttacattct
    22021 aacgaaaatt cgatcggaga aaaagattca gaagaaggac aacatggaca gttaccaaaa
    22081 accgatccag aacaatatcg ttataaatct aacattaatt ctgaaaatga aaagcgcatt
    22141 tcagatttac caaaacgaaa tcaagaagta caaacggatg aggattccat cgatttgcca
    22201 aatgataatg gaatcgaagt tggaactgaa aaatcaatga aaattggttt gcctaagaca
    22261 caaaatttac caatgaaaga tagtcgtttg aatcaattca aggagaatga tcaaagcttt
    22321 gttcaccaat caactgcagg tactgttcaa atgaaaccaa gtcgtttgcc tgaaaatgga
    22381 aatctgacaa ctgtttctta caatttatct gaaagtggtg agacagggaa tgtgtccatt
    22441 caaaatgttg tgacaagaga agctgcacca gaacctattc aaaaagttca cacaggttac
    22501 aaaccagcaa atgtggcatt tactgcacca acaaaaataa atgtaacaag agaaactaaa
    22561 aacaggaaca atgaattgaa agaacaacta ttgagggata tcaaacagga tatcaaacca
    22621 taaaaggcct acgagttagg ccttttttct atgtatgaaa aggagaaatc atttatggat
    22681 aaatatatta acatgaaaaa tcctccaaga tttagtgggg aatggaagtt tttcgccttt
    22741 ttctttttag atctattaac gcttggtggt tgcttttttt gggcgaaaat agtcaataat
    22801 cttgtccact taccactctt ttttgcactc ttgaattatg gcttatggat tgcgatagct
    22861 ctagtgattg tttggcgacc ttataataac ccaggtcaac gacaaatttt tgcgattatc
    22921 gaatctattt tctttgcgga taagaaccac tatcatgcgt tagatcctaa ccgaaattat
    22981 catggtacgt ataaggagga tgaataaatg cgagtcaaag gacaaaattc agttgataag
    23041 aaatccattt tgcagtttgg aaaaccagaa gaacgatcta tcttggattt atttccttat
    23101 aaatcctgta cagaagaggg agtcttagtt actaaagaag atcattttca acgttattat
    23161 cgtgtagttt ctacagacgt ggaaggatta aacgaacaag agaaactaga acgaatgaat
    23221 cagttgacca ccgtcatgcg tacgtatgtg caaacaatta agttagttag tttgaccaca
    23281 gagacggatc taacggaaca aatcgtacaa aagcggcaat tattagataa gaatcgtatg
    23341 gaacaatcct taggaagaaa tctacagcga ttaagaaaat atgaaaagaa attagtagag
    23401 gaaatccagg aattgaaacg agcagaacaa gaacgtcctg acttaagctt ctttttcgtc
    23461 attgaaggaa agacaatcaa ggatttacaa accagaaatc gtcagttaat gcgatctagt
    23521 ggcattttag ggttaaaacc actctacaaa aaagaattga tcaagattct ttatcgaatg
    23581 aacaatatga atgatgagta ggaatggaga agagagaatg aaatatccgt tgatatggtt
    23641 agaaaatgat gttccgaagg aagagatcac tagaaaattt tctcacactt taagcctggg
    23701 taagttggga aaagggcaat cttttaacca agtaaagaat caaacaagaa gaggtgactg
    23761 tacatgttag aagcgattaa aacagctttt tcaccacaaa atgatcaaaa agtacttgat
    23821 ccattaaagc aactaggaaa tcgggaaata gaaattttaa aatccaaagg gtatgattta
    23881 gattttttaa aacaagtgat gccacaaggt ggattaaatt tcgatgaaga gtatgtgacc
    23941 tatggaaatg gttattcacg atgtgtgaca gtctatcact atacaaaaaa tcctactctt
    24001 ttttggttgg ccaatctcat gaacaatcct catacagtgg ccacactaga tgttagcaca
    24061 gatgaaaagc aaaaagtgat tgatcaaatc aaccgcgcat tagatgaatt aaatgatcga
    24121 tcagataatg aacgaaaagc gactgaccga aataaaagtg cggaagaata cctggaacta
    24181 gcggaatatg cccgaaaatt gacttctaat ggggaaattt ctaaacaagt caaagtgaga
    24241 gtacatgttt acggaaatac gatacaagaa gtggatgaac gggcgcagga actgatcgaa
    24301 aatctaaaag ggatggatta tcgtgcggtg acttatctgc ataaaaccaa acgagattgg
    24361 caatcgatgt ttaccatgtt cgatgaacaa gctgaatggt tcggaaatat caaagggcaa
    24421 tcactaccat cgttaaacat tggcggaggt ttcccgttcc accaccaaac gctaaaagat
    24481 ccaggaggta cacaatatgg ggaaaccatg acaggcggcc catttgtctg gaatgcgacg
    24541 tataaaaata gtgtacggtt gtctactaat accttgcttc taggaatgat gggagctggt
    24601 aaatcgaccg ttcttaaaat gatcgcggaa gcccatttag ctgcaggaga ctttatttgg
    24661 ggctttgaaa agggaaaaga ctttattcct ttcttaaagg aatataacgg tattatggtg
    24721 cgtttagacg gatctgacgg gatgatcaat cctttagaga tatttgccac acggacgtat
    24781 gatgaagcat ccaggttata tgacgatgga tcagtcaaag atttgaagat caatgaagca
    24841 gcgtcctatc aaacgcactt agacaaagta gtctatcagg tacagttagt gagtcctcag
    24901 ctaaaaggaa cgatgaaagc agaatttaag acctacttaa atcgttttta tgaagagtat
    24961 ggaacagtac cacgtggctt tacttcctca aattcgcgtt caaatactga tacgcaagtc
    25021 acaggtaaag atccagaagc ttatccgacg ttcaaagaat ttttagactt tttaggacaa
    25081 ctggaattac caggtgcttc ccaagagaaa aagaatcgaa aagaagaaat ggaatccatc
    25141 gtagaatcct tatgtgagac gtatggcatg atttttgatg gtcacagtac gattcgacac
    25201 cttgaccaac aacagttggt cttttttgac attgactcta ttacaggttt agatcgaggg
    25261 gtgcaacaat gtcaaatgta catggctatg acgttgattt ggaatcaggc attgatccag
    25321 ggaagaagac aacgacgttt aattaaagaa aaacaaatgg aagaacaaga cttcaaacgc
    25381 ttcttggcga tgattgatga gtgccataat gcgattaatc cagattttat tgaaacggta
    25441 aattatgtca caaactttca acgagaaatg cgtaaagtag aagcaatgac tgttttagct
    25501 acacaatccc cacaagaaat ggtgccagaa aatatgtctt ctgataactt ctcgaaactg
    25561 aagaaagtct ttgaattttc ttctacgaag atctttatgc gtatggatga atctatttta
    25621 cgtcatatag aaggactagt gggtaaatca atgacgaact cagaattaga atccattcct
    25681 caacaaaatc aaggagacgc tgtgatcaac tttggatcaa aagaaaccat tcgcgttcat
    25741 tttagtccga acaaacgtca attaaaattg tttgatggag gaaaataatg gaaaatcaag
    25801 atgaaagttt aatcaagcag tatgttaagc gtcgcgcaaa gcggcgctta tttttgtggt
    25861 tgtttggtac ttctgcaggt ttgattacta ttttgatcac tgtgtttgtt accctatttc
    25921 taatacttgc ggcgggttcg attgataatt cggattcaga ttcaagcagt ggtggcgaag
    25981 cttttacagg agaatattcg gaaggcttac cgatttataa agaaatcaaa ggaagaggcc
    26041 ctttttctga tgagattgcg caatatgccg taggtgcagc agtgaaatat aaattattac
    26101 caagtgtgat tctttcgcaa tacggttatg aatcagcgtt tggtacgtcg gcgtccgcaa
    26161 gaaatgatct caactatttt gggattactt ggtttgatgg ttgtctgttt cctaaaggaa
    26221 ctgcacgagg aattggtggc attgaaggtg gctggtacat gaagtttccc aactcaaaag
    26281 ctgcattctc ctattacggc tttatggtag ccacacaatc aaattttaat gcatgtgtag
    26341 gaaataaaag ccctggcgca agcttattga tcttaggacg aggtggttat gccgctgcag
    26401 gaattactga agatagtccg tattatacag gttgtatgtc gattattacc tcaaataaat
    26461 taacagaata tgatgaattt gcgatcaaac attggggaga aggcggaaat gacaacggta
    26521 cgattactgg tgaatggacg aatcctttcc ctggcagcag tttagataaa agttccttca
    26581 gcggtggtca attatttgga acgaatccag gcggtgaatt ccgtccgaat ggtttccatg
    26641 atggattaga ctttggttca gttgatcatc caggaagtga gattcatgcg gttcatggag
    26701 gaaaagtcgt gtacgttgga aatcctggta tttcaggttt gggcgcgtgt gtgatcgtga
    26761 ttaattacga cggcttgaat atggtatatc aagaatttgc aaatagtaca ggaaattccc
    26821 gtgtgaaagt gggagatcaa gtgaaagttg gtcaagtaat cgggatacga gatacagcac
    26881 acttgcattt aggcttcact cgaatggatt ggcgtcaagc acaaggacac gcattcacag
    26941 atgatggtac atggatcgat ccattaccat ttttgaatag cagcaaaaaa tagatcagga
    27001 gaaaaaatat ggaaaataaa acgttaaaaa tcgtcttaac agctgcactc agcattagct
    27061 taattggcaa tatcgcgctc ttttggcagc tgaaagagga acaagcgaca aatacctata
    27121 aaattgaaga agtggctaaa aacgcgtctg agaagctcaa aaaaaatttc tctgaggcac
    27181 aggcgaagat cgacaagtta aatcaaacca tcaatgagaa aaaccaagaa atcgatcagc
    27241 tgaagcaaca agacggtata aaagcaaaag aagaagtcac agatgcccaa agaaagacgg
    27301 cagaagagtt cggtcaattg gccattgata aatcactaag cagaaatgaa ttaacggaaa
    27361 aactaaaaga tgtcgcaacc caagaagtga tcgacaaatt aagtcctgca gatgatgatc
    27421 accaacatga tgattatgga agttactcct ttacattagg tgacgtgcaa acttacgctg
    27481 aaacaagcag tgtaaaagag gaacaaaatt ttgtcgtatt tgttgattat acaatcggca
    27541 atcctcaatt caaagatgtc aaaccgcaaa aaataaaagg tggattaact gtcactgaaa
    27601 aacaagtaga tggtcaatgg aaagtcacag actttagtta tttcactcgt taggaggaag
    27661 aaaatgagta agaaaaaagg agcaagacaa cgttttaaaa aacggaaaag ttatcttaga
    27721 tggctgggaa tctttacgat tctcggcttt ttgattcttg ctagtccgat ttatacgcat
    27781 aagaaggtaa acataccaga tgaggtaagc acgaacgctt ttttaaacac gaaagaactg
    27841 acaatggtat caaaagaaaa aggagcgaat cagcaattag tttttgaatt tgcgattgac
    27901 gatgaggatc aaggcttatt aaaagaactg gctaatcttg attatcgcgt agaagtaaag
    27961 acagctaaag gtgattatca agcaataaaa gccaaagtga tcaaggtgtc tgatgattat
    28021 tttgtagtga aattagcaca tgtaccagaa gaatatatcg ccatgcgttt aaccattgtt
    28081 cctgaaaaaa ttgatccaaa agtggatatg caagaaccac aagatctgat ttattacgtt
    28141 cacgaagata aggttaagga tcaagtgaaa gatgataatt atgaacaaca tgcgatcaat
    28201 tacaaagtga aaggatacaa gaaagaacaa aaagcagcac aaaaacagat tgaatccttg
    28261 caagcaacca ttgatttgaa tgaagaatta gtgaataaac taaaagacca attgccttat
    28321 caagtcgcag atgatcagga aaatactgaa aataagatta atgattacca gcaagaaatt
    28381 gaaacatcga aaacgcaaat gaaagatcaa gaagagatca ttcaaaaaat tcaagaaaaa
    28441 attgatcgta tgacaaaaga aaatatttag tagaacaggt ggagtgaaag aaatgaaggg
    28501 agcgaacttg gtcggcaaag atattttcgc tgagcagaaa gtattgatta aagcctatct
    28561 caaagttgta aaagctaaag aaatatgtca agaagtgaat ttatctaaca caacaatatc
    28621 tgagttgaag aatggtagaa agagtttaga taccttaaaa atcagatcat ttgaagcttt
    28681 gtatttagct gctaaaaaat attttacggc tgaaagaaaa taaaaacagg aggattttat
    28741 gt
//
LOCUS       DAZUMK010000023        28706 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000023 DAZUMK010000000
VERSION     DAZUMK010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.023"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..226)
                     /locus_tag="ACG808_001013"
     CDS             complement(<1..226)
                     /locus_tag="ACG808_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFS5046656.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(223..741)
                     /gene="msrA"
                     /locus_tag="ACG808_001014"
     CDS             complement(223..741)
                     /gene="msrA"
                     /locus_tag="ACG808_001014"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS5046657.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(761..1474)
                     /locus_tag="ACG808_001015"
     CDS             complement(761..1474)
                     /locus_tag="ACG808_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFS5046658.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(1477..2373)
                     /locus_tag="ACG808_001016"
     CDS             complement(1477..2373)
                     /locus_tag="ACG808_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFS5046659.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(2531..3373)
                     /locus_tag="ACG808_001017"
     CDS             complement(2531..3373)
                     /locus_tag="ACG808_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS5046660.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            3505..4158
                     /locus_tag="ACG808_001018"
     CDS             3505..4158
                     /locus_tag="ACG808_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFS5046661.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(4221..4748)
                     /locus_tag="ACG808_001019"
     CDS             complement(4221..4748)
                     /locus_tag="ACG808_001019"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFS5046662.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(4768..5715)
                     /locus_tag="ACG808_001020"
     CDS             complement(4768..5715)
                     /locus_tag="ACG808_001020"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFS5046663.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(5727..7610)
                     /locus_tag="ACG808_001021"
     CDS             complement(5727..7610)
                     /locus_tag="ACG808_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS5046664.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            complement(7773..9851)
                     /locus_tag="ACG808_001022"
     CDS             complement(7773..9851)
                     /locus_tag="ACG808_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFS5046665.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(9864..10139)
                     /locus_tag="ACG808_001023"
     CDS             complement(9864..10139)
                     /locus_tag="ACG808_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046666.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(10501..10956)
                     /locus_tag="ACG808_001024"
     CDS             complement(10501..10956)
                     /locus_tag="ACG808_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046667.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(11110..12318)
                     /locus_tag="ACG808_001025"
     CDS             complement(11110..12318)
                     /locus_tag="ACG808_001025"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFS5046668.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(12430..12762)
                     /locus_tag="ACG808_001026"
     CDS             complement(12430..12762)
                     /locus_tag="ACG808_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFS5046669.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            12910..13785
                     /locus_tag="ACG808_001027"
     CDS             12910..13785
                     /locus_tag="ACG808_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS5046670.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(13867..14448)
                     /locus_tag="ACG808_001028"
     CDS             complement(13867..14448)
                     /locus_tag="ACG808_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFS5046671.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(14454..15713)
                     /locus_tag="ACG808_001029"
     CDS             complement(14454..15713)
                     /locus_tag="ACG808_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFS5046672.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(15715..16761)
                     /locus_tag="ACG808_001030"
     CDS             complement(15715..16761)
                     /locus_tag="ACG808_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046673.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(16996..17271)
                     /locus_tag="ACG808_001031"
     CDS             complement(16996..17271)
                     /locus_tag="ACG808_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFS5046674.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(17514..18824)
                     /gene="der"
                     /locus_tag="ACG808_001032"
     CDS             complement(17514..18824)
                     /gene="der"
                     /locus_tag="ACG808_001032"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFS5046675.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(19006..20235)
                     /gene="rpsA"
                     /locus_tag="ACG808_001033"
     CDS             complement(19006..20235)
                     /gene="rpsA"
                     /locus_tag="ACG808_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFS5046676.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(20370..21050)
                     /gene="cmk"
                     /locus_tag="ACG808_001034"
     CDS             complement(20370..21050)
                     /gene="cmk"
                     /locus_tag="ACG808_001034"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFS5046677.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(21099..21734)
                     /locus_tag="ACG808_001035"
     CDS             complement(21099..21734)
                     /locus_tag="ACG808_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFS5046678.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(21800..23203)
                     /locus_tag="ACG808_001036"
     CDS             complement(21800..23203)
                     /locus_tag="ACG808_001036"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFS5046679.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(23190..24221)
                     /locus_tag="ACG808_001037"
     CDS             complement(23190..24221)
                     /locus_tag="ACG808_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5046680.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            24252..24473
                     /locus_tag="ACG808_001038"
     CDS             24252..24473
                     /locus_tag="ACG808_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFS5046681.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(24638..25375)
                     /locus_tag="ACG808_001039"
     CDS             complement(24638..25375)
                     /locus_tag="ACG808_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5046682.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLILYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(25528..26868)
                     /locus_tag="ACG808_001040"
     CDS             complement(25528..26868)
                     /locus_tag="ACG808_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFS5046683.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(27105..28628)
                     /locus_tag="ACG808_001041"
     CDS             complement(27105..28628)
                     /locus_tag="ACG808_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFS5046684.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
BASE COUNT         8024 a         6056 c         4873 g         9753 t
ORIGIN      
        1 atatgtcatg attattttct aaatattttt cccatgcttc gtcaaagata tccatggacg
       61 gcaaatagtc gacattcagt tccagataat tagaaatttc ttcgtagcta gagctgtgct
      121 taggaaattg gatgtcttta ccggcttctg tagcgaaaac agataaagca tcatgtgtgc
      181 caccgcctcg cagtgtcatt aaataatgaa aaaagcttcg attcaccctt cgttcctcca
      241 attttcttcg ataaaggcag cccggcgtgc atggtttctt ttaaaatttt caggatgctt
      301 tttataatac tcttgatggt cttcttctgc tagataaaat ggttgggctg gttcgatttt
      361 tgtaacgatt ggctgttcga aacgtccact ttcagctaac tgtttcttac ttttttcagc
      421 aatgatccgc tgttcttctg tcaaataaaa gatcaccggg cgataattgt caccgcgatc
      481 ctcgaattgt ccgaaagcgt cagttggatc agtttgctgc cagtaaagtt ctactaattg
      541 ttcatatgaa atcaatgttg gatcatagat aatctccact gcttctgtat gtccagtcgt
      601 ttggcttttt acttgttcat aagtcggatc tggtacatgc cctccagtat atcctgacat
      661 cactgtatgg ataccaggaa gcgtgtcgaa aggctggatc atacaccaga agcagcctcc
      721 tgcaaatatt gctcgttcca ttatgattcc ccctctaata ctactcatcc gatgattttt
      781 tattttcttt tgtcttgctt tcagtactgc tggtgctagt tttgttggta tttgagttgt
      841 ttgaagcatc agaggtatct tttggcaaat atagactaaa ccgaatgtca tcatttacta
      901 agtcgatttt atctgcttta atatacatcc cgttttgcat tcggaattga tccaacctaa
      961 gcatgatagt cagatcatct gtattgactt cgatccaatc tggtaattta tagtttctct
     1021 tgatcatatt catcacatct ttgattggca aactcaatgt tccgatagaa agacttctag
     1081 cttttagttg gacatttccg ttttccatca catacggatc aaaatataaa tagaaatcga
     1141 taggaaaacc tagtacttcg aaagtaccat tgagtagtgc ttcattttcc aaataaaatt
     1201 tatattcaat atcactatct ttttgatact cactaaggaa aaagctgatg atttgattga
     1261 ctttttcttt atttgaattg atcgttaaga caggatctcc ttgtctttcc gtaagagctg
     1321 gtatttctgg gagttctggt tcccgattag caaaaatacg gctgcctaaa aatacggtcg
     1381 aaccaagtac taccccaaga aggataagga aggcgaattt ccagtaattt gttttctttg
     1441 ttttgtttga agatacgtca gatcttgttt tcatgtttat tgatcatcct ccttctctaa
     1501 ccattggttt tgtgtctcga tcagctgatc tctcactgca tttgccatca attgataacc
     1561 taaattattt ggatggaaat gatcttcttc atagagcaca ttatttttga tgccatttag
     1621 attttctgtc gtgctagttg tttgatctgt tccttcttct gtgacaccta cttcctgatt
     1681 caatccttga taaagcaaat cattgattgg gataaagtag cagttcggca ttttttttgt
     1741 caccgtttct gttcctttgt tccaattgtt aacaattgtc tgcatgtcag tgatctcagg
     1801 aaagttcaaa taaaatggat tgtagatgcc taaaatgtaa ataggtgcgg ttttattgag
     1861 ggatcgaaat tcttccaata gttctgtgac attttcctga tagtttttca gtggcttttt
     1921 aaaggaattg accgttaacc cgaataaatt attttggata actttcatca aatcattacc
     1981 gccaacagtc agtgtgataa aatcagctgt tttgatattg tttcgaattt cttcttttgt
     2041 ttttactcgc tttaagatct ggtcacttcg ttcaccattt acaccatagt tttctgtttc
     2101 aaccgcggtc aaattataac ggtcttttag atcatttgcg acaaggggaa cgaatcctcc
     2161 ttgcttggtt tgatctccta ctccttcagt cagcgaatct ccgatagcta tgtagtgaag
     2221 tttttctttt ttatttggat cttctctcac agtagttact ggtttcatta gtgggccagc
     2281 tttcggtata gaagcagcta aacctgcata gatgatgatc gcaattaata atgggaacaa
     2341 aacgaggatc aaatgtcgtt tcgttaattt cattttttca cctcttgtat tcgattggtt
     2401 ctaatgttat caaaaatttt atcaaaaagg tagtctttat gaaaaaagaa aacaattatt
     2461 ttcctaattt tataaagaaa aagagaataa gctgcaagcc tttcaggctg agggcttatc
     2521 ctcttttcga ctaatctaca taatacatga ttgcaaacgc gtctttacca gtatgcgtag
     2581 cgataaccgg attcgtatgc agtacaggga tatgcatgtc cgggaataat tcttgcaaac
     2641 cctctttgaa ttcttgcgcc aattcttttg catccgcatg tgaaataccg atttgtcgca
     2701 catttggcaa ttctttcagt tcatttttca aattattaaa ccatttatcg aacgttttct
     2761 ttcctcgccc cttagttaca gggattaatt cagaatgggc gaaattcatc accacgcgta
     2821 tgttcaaaac actagacagc aaaccagttg ttcggctgat tcttccacct tttaccaagt
     2881 tatctagtgt ggatattccg atgtataatt ttgtattgtc ttgtactttt ttgacttctt
     2941 caaggatttt ctctactgtt ttaccttctt ttgctaattc agctgctcga attacctgaa
     3001 aagataatcc ttggtcagtg gtatcactgt cgataaccgt tactttcgtt tttgttagat
     3061 tactggcttg tcttgctgct tctactgttc cactcaagcc ttttgtcata tggatcgaaa
     3121 tcacttcact gccgtcttct cctagctcat catacatcga gatgaattct ccaattggtg
     3181 gttggcttgt tttgggcaga ttttttgctt tcgccatcat ttccataaat ctttcgccgt
     3241 ctagttggtc atcatcggga tatactgttc cgtcgatcat gatggatagc ggcatgacac
     3301 gaatgcctaa agcatcgcgt acattttttt ccatcgtaca tgaagaatct gttacgattt
     3361 ttacatttgt cattattttc tctctctctt tctttaacaa gatgccttat tcatgttaaa
     3421 ctacatgtaa gacaatttct atttttagta taacagacaa ataatgattt ttcattaatt
     3481 acacctagta aaggagccgt tttcatggaa aaaacgaaat ttaatcgaac gtatgaaata
     3541 ttaaatgaag tgtttaatgc agtcacccat ggaattggga ctggattgag tatcgcggga
     3601 ctagtggttc ttttagtaaa aggtgcccac ttgcattcct ccctccatat tgtctcttat
     3661 gctatctatg gatcgatgat gattctttta tttttaacgt ctactttgtt tcattcattg
     3721 atttttacaa aagcaaaaaa ggtgttccaa gtattcgatc atagttcgat ttttcttttg
     3781 attgcaggaa gttatactcc tttttgtttg ctgagtatcc gaggatggct aggttggact
     3841 ttatttgtat taatctggtt gtttgcgatt cttggcatta tatataaagc gttgacgttg
     3901 cataaacggg agagagtatc aaaagtatct acgatcatct atatttttat ggggtggctc
     3961 tgtgtcgtgg ctgctgttcc tctttatcac tctcttggaa tgactggtgt tattttgatg
     4021 gtagcaggcg ggctttctta cacgataggc gcattctttt atagcttgaa ttctatccgc
     4081 tatatgcatg ttgtttggca tttgtttgtt atgttaggtg caggttttat gtacttctct
     4141 gttttattct ttacttaata aaaaaaataa agctatggat gggacagctc taaataacag
     4201 ctagtctatc catagctttt ttatatcgtc aattcgcctg tcccgctttt tacaggattc
     4261 tcattacgct gatacgtttc aaattgatgc ggataacgat ttttttcatc catttctcct
     4321 tgactagtat tgatcagtga ccaatcagac cagtcaacag gtggaaaata agcatctcca
     4381 tcgaatacat gatggatgac tgtacggtat aatatcgtac aaaatggcag aaattcttga
     4441 taaacagctg accctccagc gatgaaaaag ataccgtctg tttctttttc cagagcaagc
     4501 acgtcttcca cactatgcat gacatgcacg ccttctgctt tataggaaga atctctcgtc
     4561 aaaacaatcg tctgacgatt cggcaacgga cggctaccca tcccttcgaa tgtttttcgt
     4621 cccatcacta gtgtatttgc ttctgtcatt tgtttgaaga atttcagatc atttggcaaa
     4681 cgccaaggaa gacggtcttc ttttccaatc aatccgtttt catcctgtgc ccaaattgca
     4741 gctaacattt tctcatcctt tctgattcta aacagcgata ggagctttga tcgccggatg
     4801 tgggtcatat ccttctaatt ggatatcttc catctcaaaa tcaaatactg actgcttttc
     4861 ctgattcaaa actaaagtcg gaaatgcgcg tatttctcgg ctcaattgtt ctttcatttg
     4921 ttcgatatga ttgatataaa gatgagcatc tcctaatgtg tgaacaaatt cgccaaccgc
     4981 taaacctgtt tcatgtgcaa ttaggtgagt cagcaacgca taacttgcaa tgttgaacgg
     5041 aacgcctaga aacacatccc cactgcgttg atacaattgg cagcttagcc gtccctcttg
     5101 aacataaaat tgaaacatcg tatgacaagg aggcaaagcc atgctaggaa catcttccgg
     5161 attccatgca gaaacgatca atcttctaga atcaggagtc tttttgattg cttcgatcac
     5221 ttgtttgatt tgatcgataa agccaccgtc ttttgtttcc cagtgacgcc attgatagcc
     5281 gtagatgtgt ccaagatcgc cgtatgtttc agcaaaggaa tcatcggtta ggatcttctc
     5341 gcaaaaaagc ttgctctgct ttttatattc ttcattgaat tcaggatctg tcaaacatct
     5401 atggccaaaa tcagtcatat ctggtccatt atagtctgga cttttgatgt agcgttcaaa
     5461 tgcccattca tcccaaatat ggtttttatt ttgtaataga tagcggatat tcgtatctcc
     5521 ttttaaaaac caaagcaatt cgcttttgat caatccaaaa ggtactcgtt tcgttgttaa
     5581 caaaggaaac cctttttgca gatcaaaccg catttggtat ccgaataagc ttaatgtgcc
     5641 tgtgcctgtt ctgtcttctt tacgatgccc ttcttccaac aattttcttc ctaagtcaag
     5701 atacgcttgt tccatccttt ttcctcctaa tcttctaccc attcactcaa gtattcccac
     5761 cgtgccattt tttcttcaag ttgtgcttct gtttcagata cgtcgttttg caattcttgt
     5821 aatcgagtga aattatctcc ttgatgattc atctcttctt ggagcaatga gattttttct
     5881 tcaagttctg caatctcatc ttcaatcgtt tcccattctt tttgttccat gtaagataat
     5941 ttcttctttt cagcaggttc cttgctctgt atttttggtt tcgctttttc agcaggctgc
     6001 tgggtctctt tttgcttcag taaataactg gacatgtttc cgtaataaag ttcttgtttt
     6061 ccgtttcctt ggaatactaa taacttatct gctaccttgt ccaagaaata acggtcatgg
     6121 gaaacagtga tcacagcacc tggaaaactt ctaaaataat cttccaaaat cgtcaatgtt
     6181 gctatgtcca aatcattggt cggttcatcc aataataaaa cattaggttg ctgaatcagc
     6241 agtttcaaca aaaataaccg gcgtttttct ccgcctgaaa gtttgcctat cagtgtccca
     6301 tgcataaatc ttggaaataa aaatcgttca agtaattctg ctactccgat ttgactgcca
     6361 tctgctgttt ttgcttcttc agcagcttct tgtaagtaag caatcatccg tttatctggt
     6421 gccatttctt cattttcttg tgtataatac gcaagatgaa ccgtttcacc gatagacatt
     6481 gtaccgctat ccaggggaat tctgccagct agaatattaa gcaatgtcga ttttcccgca
     6541 ccatttttac cagtaatacc tagtctttcc cgagattgaa tcaataaatc taaatgttct
     6601 agcagtgttt ggtcatttat acggtaactg ccatctttga tttccagtac ttttttccct
     6661 aaacgttgtg tcgctaggtt cagctctaaa tcatcttctt ggtttacttg gtacaagttc
     6721 tcctttagat cttcgaaacg gttgatccgt gcttgctgct tggtgccacg tgcctgtacg
     6781 ccagccctca tccaagctaa ttcctgttta taaagccgct tgcgcttttc ttcttgttct
     6841 acttctacac gatcacgttc tgctttttct aagacgtaag cttcataatt tcctttatac
     6901 tcgtataatt ttccaaatga aagttcgaag attcggtttg ctactctatc tagaaaataa
     6961 cgatcgtggg taaccatcaa tagtgcgcca cggtattgtt tcaaatagtt ttccaaccac
     7021 tcgatcgctt cataatctaa atggtttgtt ggttcgtcta gtaatagtaa atcgggttca
     7081 tcaatcaaca cttgcgccag actaactcgt tttttctgac cacctgataa agtactgatt
     7141 tttttatcta attcactgat tcctaatttt tgcaaaataa tctttgcgtt ggtatctgta
     7201 gtccaagcat cttctttatt catttgttct tctgctttag cataacgttt ttgcacatca
     7261 gggtcttctc cgttttcccc tagttcgatc aatgcttcct catatgctcg aacagtttgg
     7321 atcaaggggc tgtttccttg aaagactgct tggagaacag ttaattcatc ggaaaattca
     7381 gctgcttgag aaagataacc aatacggtaa tcagttggat aaaacactgt ttgccgatca
     7441 ccgtcgctgc tatcgatacc ggcaataata tttaagagac ttgttttacc cgtcccgttc
     7501 gtcccaatca acccgatacg gtctttttcg tggaccaaaa aagaaatctg gtcaaacaaa
     7561 tctttttcac cataagtttt tttgaggtca gttactttta attctttcaa catgttccct
     7621 ccttatcttg gatattgtat cataaaaata gtgaacttca ggaaaatttt tttggtattt
     7681 gttctttttg tgttctcaaa aaaagaacag gaccgattcg atcctgttct gatatgtttt
     7741 gttttttata aaacttgaaa atttttacct ttttatttta ttggactcgt tttttttgac
     7801 tttgctcgaa acgcaaatcg gaaagaaaag ttatcagatg gtttgttcat cgtataagat
     7861 tctaacaaat tgatattcct gcgatagctg ttcgcagaaa acgtcgtgat tttttcagca
     7921 acttcgtatt catcaatgat ttttctttca agataataac ctcttagcat ttttttcgta
     7981 aacagactat cttgctggat catccacgaa gaaatcaaat tccccgagtt catttttatt
     8041 ccttcaagtt tgcgttgctg gataaaaaat tgaaccatcg tttcatcata aatatcagtc
     8101 aagttatcta aactttcaat aatcaactga gtattttttt gatatgtctc tctgatttct
     8161 tggatatctt catcactaat aaatgtctga cgatcagcat atcttctttg ccaaaacatt
     8221 ttcggatgga gaacgattcg cattaaccta cggacaacaa tgaaccaaaa gccgataaaa
     8281 gacaaaatct ggcttgtgat agaatgttcg aatcgagaaa gaaaacggga gtaaaaacga
     8341 taaccattag aagaaagctt gcccgaacga taactttcat ccaatccatc ccgttcgatc
     8401 gacaaaatca gtgcttgtat ttcttgtact tcttgctttt catcatcggt taaatcttcc
     8461 aagtacagat cacgtatccg ttcttgataa ttttctatca ctgcttcagt ggctaaaaat
     8521 tcttttgtat tacgatcaat ttcctttatt tcttttcgca aaacttcaac gacctcttct
     8581 aaaatcatca aagcattcaa atccgtgctt ttcgcttcct ctgtttccgt cagcataggc
     8641 aagacaatga tccctatgac caaagtcact aagatgacac ccgccgtcag aaacaaaagc
     8701 aaagaccgct ggtaaaacat catattattg attgaaaatg gcaaaataaa aatcgtagcc
     8761 aaactgactg ttccctttac accgccaaat gttaagatca agatctcgtt caactgtttt
     8821 ttgaactttt tactgccatc tttgaataca taaaaaagac tgatagaaat gaatctgatc
     8881 acaaaaagca tgacagagat taatacgata atcgctaata aaagaccgtt tggatataaa
     8941 ccgtctcccc atactggcga gaacacttgc gtcaattcga ttcctaaaaa tataaatact
     9001 aatgcattca gtgtaaaagc aatagttgtc catgtactgt gggacaagct cgacaattct
     9061 gcatcaaaca cagagatttt cctaaaacca gaagcttgga gaatacctgc tgccactgca
     9121 gcaatgattc cagatacatc aaatgcctct gccaatacat aggctagaaa aggaagcaaa
     9181 agttcgatca gcagataccc tgtgacatct tgtgctgatg ctttttcgat caaatgaatt
     9241 attttttgtt tgaaccagac aagcaaaaag ccgatcaaag ctcccccaat actagaaact
     9301 actagactca tccccgcgtt tactgctgaa aaagatccgg tgatcagcgc accaagagca
     9361 aactggaagg cagtaacacc agatgcatca ttcaaaagac cttctccttc caaaatatgc
     9421 atcgcctttt tagggatatt cactcgtcca gaaagagaac tgactgctac cgcatctgtc
     9481 ggacctaacg ctgctccaaa agcaaagcag gcagcaagtg ggacacttgg caacaggaat
     9541 gataacgtcg cacctacccc gacaagggtc aaaatgactc ctccaaatgc caagaacaaa
     9601 attgtcccga aatttttgag aatcgaagaa atatctgctt tttctccttc tctaaataac
     9661 aatggtgcaa taatcataac taagaacaat tcaggttcaa aatcgattga ttgtccccac
     9721 tctgtcagac ctaataccac tccaaggaaa atttggatca gtggagctgg aatcattggc
     9781 aaaatgcggc tagctatgtt cgataaggtg atcgttactg caaatacaat aactaactca
     9841 gcaaattcca tcgtccatcc cctttatttt ttattttgtg taagcatttt taaaaattgt
     9901 tcttttttct ttaaaatttt ggtttcgact acttcaacct gtgtttggtt cccctctaat
     9961 tcacactgta cacgttttga ctccaaacga agcagatcat gtttcagacg ttccaactga
    10021 cgatacatca atatatttct tttacgcaac ttacaaagta tttgacggcg gtcatccatc
    10081 gaaagggcgc catagcgatc atgcaacagt tttttttgtt gttcaaaact ctctctcatt
    10141 ttccagatct cctatacaca aacttccatc attatagcaa agttcttttt ggatgaaaaa
    10201 tatttcattg tttcatcgtt tattgctagt tagaaatttc cataaatttt tcaacgcctc
    10261 attctattcc aaatagtatc agatatcaag taaaacttaa gaaagataaa agtttctgac
    10321 aaaaggatgt ttactttccc attcctgcct ttcttgttct tttatcacaa caaaaaaatg
    10381 gggctgcgac aaaaatcttg tcacagtccc ttattctgaa taaacggtgt ggaagagaca
    10441 gcctatcagt agtaataaga cacatctctt tcacagcctc atttttgcgt aaaaatcgat
    10501 ttattcgaaa aattccccgt atacatcttc tacacgttga tctggattga tcacgatata
    10561 caagttacct gttttttcgc tgatttttgt tgattgatat tgattgtcta atccttctgt
    10621 tcctttatct agatatggga agtacacaag atcaggtgtg ccatttcggt aaaggatttc
    10681 cattcgctcg atatcttcgt atttcaatgc gcgattaaac atgcccattt ccagtccgcc
    10741 taggttgata tcatgtgtcg caactgcgtc cccttcatgg aagatttcga ttttaaatcc
    10801 agcacacgga tggatttcaa caaattcact gccattgatt cgtccaaaac ttgttgtgat
    10861 ttgtttgatc caaagatcgc cgatatgacg gcggttgatc gtccaggttt ctccatttct
    10921 aaaataaaat tttagcgctg tcatttcttt actcattaaa aagactcctt tatgatgtaa
    10981 tttattcttg tactacctca gtttagcaca aatttatgta agtggtcaca tcttatgttt
    11041 tttcttctaa tgacgtatct gcccacttta agtagccaac aaagctgagc agatacagtc
    11101 tagtgttttt taaatcaatt tttctttcga taaaaaatcc aaaacctgtt ttttatcatt
    11161 ttttattctt tgttctacga cggctttttc agctaattgc aaagcttcac taacccactt
    11221 accaggtttt tttccagttt cttctagcaa gattttacca tctactgcta attcgcttaa
    11281 tgacttgata ggtaaacgat cataggattc tcctacggca tccatctgag gtgtttcgtc
    11341 aaaatagatc atcaattctt ctacttgcag tgcatgtttt ttgcctaaga agtacaattc
    11401 gaatggttcc aaagctccag tttctcgttg tcgtagtcct tggacaagtg cttgaacgtc
    11461 tttaatcatt tggttagaac acttccacga tttcaaaaag gaacgaatct cattttcttt
    11521 tagatccagt tggctgatca ataatgccca tgcttggctc tcttctttca atgggacgga
    11581 tggtaattca gcaaaacgta aaagtgcttc gccttcttct ttcaaaccgg gacagcaaat
    11641 gtagcactct gtttcaataa atgcatggat tcccgcggcc cgttcatttc ccaagagcat
    11701 cttgatgaat tccacattta tacgttctac agatatttta cctaatagct cgtggttttc
    11761 tttgatggcg tagaaggttt cttcctctaa tttgaaccct agttgactga caaaacgcaa
    11821 cccgcgcatc attcgtaagg catcttcatg gaaccgctca tgaggattgc ctacagctcg
    11881 caagatctgt tcttctagat cttgcagacc atcgaacaaa tcgatgattt ctccatcttc
    11941 tttcaaagca aacgcattga tcgtaaaatc tcttcttttc aaatcttctg ccaacgaacg
    12001 aacgaattcg acatgatctg gtctacggta gtcctgataa gttgattccg tccgaaatgt
    12061 ggtgatctca tattgttcat cattttctaa taccaacaca gttccatgtt cgatcccaat
    12121 atctatcgtt tttggaaaaa gctgtttgat ttcttctgga aatgcgcttg ttgcgatatc
    12181 cacatcatgg attggctggt tcagcaagat atcacgaaca cttccaccta caaaataagc
    12241 ttcatatcct gcattctcta attttttcag aactggaatt gccttttggt attccagagg
    12301 taattgtttt aatttcattt ttccaacctt tcctgcgtgt ttcttgtcct gagataaaga
    12361 ctcagttgca tggtgactgt ttgacaagtc cgtttcagag tcttcaaggc caggaccact
    12421 tcatcttgtt tactcgtcct tacgctcaaa gcgattatgg tctctttgat tgaatttttc
    12481 catgtttttt tcaaaaactt cagttaagtc gatattcaat gaattagcca tgatcatcgt
    12541 aacgaataaa acatcaccaa gttcttctgc taccgagtta actggttcag aagccttttt
    12601 gcttttttct ccataagtgt ggttgacttc tcttgccaat tcacctactt cttcagttaa
    12661 tcgagccatc tgagctaatg gagagaaata ccccaccttg aattgctgga tatattcatc
    12721 cacttcctgc tgcatactgt ataaagaacg ctttttatcc atctttttcc ctccaaaact
    12781 atcttaccaa aactttaacg caaatcctag ataagagcaa ttgagaaaat cgaatttcca
    12841 tgctacattt aggaaggaat ttctaaatgg aaattctgtg aaaatacata attgaaagct
    12901 ggaattcata tggtcaaacg aaaaacagta aaagaagttc tttttatcat tataggaaca
    12961 agtatttatg catttggtct tgtttacttg aacatcgcta atcatttagc agaaggggga
    13021 gtctcaggga tcaccttgat cttaagagct ttatttggta ttgatccagc ttatacgact
    13081 ttattaatca acatcccatt gattttacta ggaggaaaga tcctcggtaa acgttccttg
    13141 gcctatactg ttctaggtac actttctttg tcattctttt tatggatctg gcaaagggtt
    13201 ccactagaaa tcaatttaca gcatgatcta ctgatcgttt ctctacttgc gggtttagtt
    13261 gcaggtgtag gaagcgggat cgtctaccgc atgggtggaa caacaggcgg cagtgatatt
    13321 atcgctcgga ttttagaaca aaattacggc atcactatgg ggcgttccct attggcattt
    13381 gatatcatcg tcttacttgc ttcattaacc tatatcgact tgaaacgtat gatgtatacg
    13441 ttgattgttt cgtatgtttt cagtcgtgtg attgattcga tcttagatgg cggatattct
    13501 gcaaaaggtc tacttgtcgt ctccaacaaa agcgaagaga ttgcaccact actgatgacc
    13561 ggtctgcaaa gaggtgtgac tttcctttct ggagaaggcg cattctctgg taattcaaaa
    13621 aaaatcatct atatggtcgt tagctctagt gaattaactg aagctaagag gatcgtccac
    13681 gagattgacg aaaaagcttt cctttcaatc atcaatgtgc atgaagtgga agggcaaggc
    13741 tttacttatt tgaagccaca gacaagatta ctgaaaaaac tataagaaaa aagacaaaga
    13801 taggctatga ggaaagattc cacatccagc tgacctttgt cttttttagt actatactat
    13861 ttttaactaa ttgttatgtt cacttacccg gttacttttc ttttgtgtct gcaaaaggat
    13921 ctttcggtta acttcatcag ataatttcaa aaaagcatcc ctatctccat cttctagtgc
    13981 ttgatcgatt tgagcataaa gcatttttag ttttgcctct tcttcttttt cttgaaaata
    14041 tcgttcaatt aattcagtat cttcttcact gactgaagaa tcccaagaag aaaatggatt
    14101 gtcttctaag atagatagat attctcttgt ctgccaagca ttttcgaata tacattccac
    14161 atataaaggc tttttccaat tcaaacggat ttcatgaaaa atctggtctg tgtccgtaaa
    14221 ttcttttccc tgcaaaaaca atttcatggg ttccccttca acagatagat cactgatctg
    14281 cagtcctcga tccgcaaaat tcgccccttc aacaaagtgg acattgctta aaatcgcttc
    14341 atgattggca agataattca agatccaaaa aacttctctt cggctaaaag atacatgatt
    14401 gaccatccac gttaaaaaat tcttcttttc ccgtacatca attagcattt ttctcacctc
    14461 tctgttaggt catctaataa tgacagtgcc tccatatctc ctggttcaag tgttaaataa
    14521 taagtcaata attcttgcgc tcttttcagc tgcccttctt ctcttaagaa aaaggcatac
    14581 tctttcaaga aatctggttc atgtgatagt tcatgataag cctgttcata gtgtacagct
    14641 gcaacggtaa agtcttctaa ttcattataa gcatgggcta ggttccattc tgcataagga
    14701 ttagcagttt cttccatctg attgatcact tgtatcacat cttcatatct ttcatcatcc
    14761 aagtaaagat tacttaaagt aagtaatgtt tcatcttgtt tgtctcctac ttctaaagct
    14821 ttcagtaaaa gttcttctgc tcgctgtcta tcatgcagac ggaaagcatt ttccgaggcc
    14881 aggtgataaa gttcgacttg gtaaggattt tctttgattc cttcttctaa cactgtttgt
    14941 gcttcttcaa tcatttcttc ttcttgaaga gcttgtccta gatatagata taacgattga
    15001 taatgcggat taacttctcg aagttcttcc agatagttga ttgcctgctg attttctttc
    15061 aattgaagat tcgtaaaagc tagctggaac agtcgatcat ctgtcctttc ttctttcagt
    15121 gcttcttcta aaggtacgat agctgcttcg aattctcctt gcatacttaa ggattgtcct
    15181 aaacgttcct gcatcgagat tcctgaaatt tcatttatcc cgcttgccaa caatgtttca
    15241 taaaccactg ctgcctcttt gaaacgatca actgaaaaat acaattcagc tagcgcaaac
    15301 tggatcaatg gttcatcagg aagcaaccca gctgcttctt tcaattttgc ttcactcact
    15361 tcagggattc cgataacttg gtacaaatca gctatcgcca ataagctttg cggatagtaa
    15421 tcactttgct tatccacttt ttccaggtat tcaaaagcta agtcgatctc attatcttcg
    15481 attgcaattt ctgctaacgg gatgttcagt ccgtcatttt ctgggtattg gttgatcagt
    15541 tgttcaaaaa tctgttttgc ttcgatcaaa aagccaatag ccagcaattc ttcgcctagt
    15601 tcagctaata tatctgctgg gtcttctttt aatgcttttt ctaaaaagat ttgtgcttgt
    15661 gacaagtctt catctgataa agcttgcaac attttttcgc tatttgtatt catttcaatt
    15721 ccctcttatc tcactgacga tttgttgaaa taccgtcatt gttctcaata attctttttc
    15781 gtttggatgt tggtatccgt ctattatacc tattttttct aaaacaagta tttttttgct
    15841 tttttctgcc aaaaaaagaa ctttttccaa atactccatc tttgaaatct gttccagcca
    15901 gtggattgga tagccaagca agtccagcca cacaaaaaag ttttttagtt ggaaatcaaa
    15961 aggatatgct tggagattcc acacgaaatg aaacatcatt cctaaaaatt ttttcatact
    16021 agcagaaagt agatggccat tttcggtcag ataaaaagct tgttcaaaca gtttaccata
    16081 atttgacagg ttttctgcct ctaattgata aaaatagatc atttcttcga tcatcccggc
    16141 aaacggccga gtcgttaatt gctttttatt tggatagttt ttatacagat tttgcaaaaa
    16201 acgatgatca caaatcattc cgcaacagat aaaagtcaaa aagtcaatct gctttccgtc
    16261 cacctgccgc tcgctgattg tttgatcata aacgatgcgt tctggcagat tgatggtttg
    16321 cagcactaca cgatttggta gctgcaaaat cgtccgctgc ggtgttaatg ctcggcttag
    16381 agaacggatc gatacaggga tcatccatag tgatgaatca agggttgtat gtttttgaaa
    16441 aaaacctgcc agatccatga tcccttcatt tccaaatccg atgataaaca tggattgatt
    16501 ttcgggatag cgtttgacga aatcaagcag gttctttaat tctgttaaat gattgcattg
    16561 agtgttggca caaatatacc agtcattgtt tgcttgattt gcaaataacg ggttgatctt
    16621 atctgcaaac aaatcataat agcgctgatt cgttaaaaat aagattggct gatcaatgga
    16681 aagttctctt agctgactga ctagcgtttc tccgtaaaac actttcgtca acgcatcttc
    16741 tacttgataa tcaagttcca tacgcatccc tcctcttctt atttttattt taccattagt
    16801 aagcaaattc tagaaaagat aagagcaaag ctcacctcat aacttgcaaa taaataaaaa
    16861 ttaaataaaa tacatgtgtt ttctatgtat gacatacgga ttcggaatct ctgtctctta
    16921 tttattcctt ttctgctagg aaataaaaag aaaagcactt gtagccctag agccacaagc
    16981 gctttttcaa ctgtcttatt taacagcgtc ttttaatgct ttacctggtt tgaatgcagg
    17041 tactttgcta gctggaattt cgatttcttc accagtttgt gggttacgac ctttgcgagc
    17101 tgcgcgttcg cgaacttcaa agttaccaaa accgattaat tgaacttttt cgccgttagc
    17161 taaagcatct tgaatagttg aaaatacagc atctactgct gcagttgcat cttttttagt
    17221 taaatcagta gctgaagcaa ctttttcgat taattctgct ttgtttgcca tggatagatt
    17281 cacctcctcc acaagagata tgatagaaat atcatttagt taaaactttc tgagtggtcc
    17341 agaagtttaa aaagaaatat tgattaactt caatattctg ttatcacgat atcatatgaa
    17401 ccgcttaata gcaaggtttt taccaatttt cacatgcttt ttttagtcat agaaacgtaa
    17461 caaacgaaac tgtctttttc ttttgaaaaa aaaacgtcga tatggtaaaa tgcctatttt
    17521 ctcctacgag ggataattcg gatggacgtc ccttcaaaag taaatgcttt tctgatttga
    17581 ttttctaaga aacgttcata agaaaaatgc atcaattcct cttcattgac aaaaacgaca
    17641 aatgtaggtg gtttgacagc gacttgtgta gcataaaaaa tcttcaggcg ttttccttta
    17701 tctgttggtg ttggattgat tgctacagca tccataatga tatcattcaa cacagccgaa
    17761 gagatacgaa gattctggtt catacttact tgttcgatca tttcaggcag acgttccaac
    17821 cgttgtttag tcactgcaga aacaaaaacg attggagcgt aatcaagata ccggaattca
    17881 tcacgaatct cttgttcgaa atcacgcatt gtattggttt ctttctttac aagatcccat
    17941 ttattcacga cgataataat tccacgacca gcttcatggg cgtaacccgc tactcgcttg
    18001 tcttgttccc gaatcccttc ttcagcattc agtaccatca aaacgatatc agaacggtca
    18061 atggcacgca ttgcacgcat cacgctgtat ttttcagtat tttcgtatac ttttccgcgt
    18121 ttacgcatac cagctgtatc tatcattaag aatttctgac cactttcaga aacgaagtgg
    18181 gtgtcgattg cgtctcgggt cgttccttca atatccgaaa caattacacg ttcttctcct
    18241 aaaatcgcat tgatcaaaga ggatttacca acatttgggc gaccaatcaa actaaactta
    18301 atcgtgtcgt cgtcctcttc ttcagtctct tcagagaaat acttcactgc ttcatctaaa
    18361 atatcaccaa tccctagtcc atggcttcct gaaacaggat aaggatcgcc tagccctaag
    18421 gaataaaatt catagatatc gtttctcatt tctggattat ctactttatt gactgctaaa
    18481 atcactggtt tattacttct gtataaaatt ttcgcaacca tctcatctgc gtctgtcacg
    18541 ccttcgcgtc cgctgactac acaaatgatt acatctgctt cttcaatcgc gatttctgct
    18601 tgatgcttga tctgatccat aaaaggttca tcgccaagat caattccgcc tgtgtcgatc
    18661 acgctgaatt ctcgacctaa ccattcacct tttgcataaa tacgatcacg agttactcca
    18721 ggagtatctt cgacaatcga gatacgctca ccagcgatgc ggttaaaaat cgtagactta
    18781 ccgacgttcg ggcggccaac gattgcaatt gttggatttg ccatattatt tcctcctcac
    18841 aagtttccaa tctcacttag tttaacgaat gcattgccaa ctttcaagca tagattgtca
    18901 attaaacgaa gtatttttat tccttattga aatactaatc gcttttcaaa agaaaaactg
    18961 agacaaaagc agctgctttc atctcagtca cagatacaaa atcaattatt tttcagaatc
    19021 atcggttgct ttttcttcta cttcttcttt caacgcatca cctaaaatgt ctcccatagt
    19081 aaatccagta ttttcttctg gtaattcata agactcaaca ttttttggtt cttcttctga
    19141 agctggtttt tcttctaaag ctttgatact caatgcaata cgatgttctt ctggatggat
    19201 ctctaatact ttcactttca cttcgtcacc ttcatgaagc acttcatgtg gtgtcgcaat
    19261 atgtttatgt gaaatttgag agatatggac tagcccttct actccaggga acacttcaac
    19321 aaaggcaccg aagcttgtca aacgtttaac tgttccgtct aatactgtgc caactggagc
    19381 ttttgtatcg atttctgacc aaggtccagg ctgtgtgtct ttgattgaca aggaaacacg
    19441 ttcttgttca ggattgatag acaacacttt gacatttaca tcatcattga cagccaatac
    19501 atcacttggt tttgctacat gactatgtga aatctctgag acatggacta gaccgtcaat
    19561 tccgcctagg tcgacaaatg cgccaaaatc agtaagacgt gcaacacgtc cactgagaac
    19621 atctccctca tggatagttg aaagaatttc ttttttcttt tcttctcttt cgttttccac
    19681 taccgcttta tgcgataaga tcaaacgatt ttctgatggt tcgatttcga tgattttaaa
    19741 gctcaatgtt ttgcctttat agtctgaaaa gtctgcaaca aaatgatctt ccaccattga
    19801 tgcagggaca aaaccacgta caccgacgtc aacgactaaa ccgcctttga caacatttgt
    19861 aacaggtgct tcgatgacct tgccctcttg gaaatctctt tcgatatctt cccaaacttt
    19921 tttagcatct aaacggcgtt ttgaaagtag atagctgccg ttttctttat ctttgccgat
    19981 cgtactgata acgactaaat ctaatacatc accgactttt actacttcgc tgatatcttc
    20041 taccggcaaa gttgataatt cttttgccgg aacgactcct tcaactccag ccccttcgat
    20101 tccgacaatc acttgtttgt cttcaatgac taaaacttcg cccttaacaa catcgccaac
    20161 ttttacttct tgtacgcttt tcattgcgtc ttccattgac tggttgttct cttgattttg
    20221 atcaaattct gtcataacac ttgtcctcct aaccaacatt ctgccgtaag tacggtactg
    20281 tttttagttt aattgattat tcaaaaaaaa taaagcgatt tcgttcgttt tccttgaaaa
    20341 aacaattgtt tttcattttt tttcttaatt taaaaaagaa gataaccttt tttcaaaaca
    20401 acagctttta ttgctgcaac gacttctgga atcgatttgc cagtcgtatc gatacgaaca
    20461 gcatcctctg cttgcttcag cggagagacg tcccttgttg aatcataatg gtctctttcc
    20521 tgtatctctt tcgtcagtgt ttcaagatca gtcggaattc ctttttcttg gttctctttg
    20581 aaacgtcttt ctgcacgctc acttacgctt gctactaaaa agattttcac ttctgcattc
    20641 ggtaatacag ctgtcccgat gtctcttccg tccatgacaa cgccaccgcg ttcccctatt
    20701 tttcgttgga cagcaactaa ttcttctctg acttttccat gtgcagagac ttcagaaaca
    20761 gcttttgtga catctggttg acggatttct aaagtcacat cttttgatgc tatgaataca
    20821 tgctgtccat cttcttgttg cgaaaaagag ataggatact ttttgatcag cgaaactagc
    20881 ccttcttcat ctgaaaaaga tacattgttc gtaatagcta aataagttac tgcgcgatac
    20941 atggcaccgg tatctgtata aatataattc aattcttttg ctaatatctt ggcaacggta
    21001 cttttcccag atgaagcagg cccgtcgatt gcaatactga tttgtgtcat gatataactc
    21061 ctttttctca gaaaaaaagc cgcaaagcgc ggctattatt atttaattct taattcttga
    21121 ccagggtaaa gcataaagtt gtttggatct aagccattca attcaaacaa ttgatcagtg
    21181 gtgatccctg cacgctcagc cacttgtttt ggaccttcgc ctgcttgaac agtcgttgtt
    21241 gaaccggctg cagctgatga agaaggtgtt gtttctgctg cattgctgct tgaatcccct
    21301 gcttgataag ttgcctcact tgaggaagtc ggtgttgtct ctgctgcaat actgctttct
    21361 gacgttacac ctgtatcttg agacacagta gattcttccg cgattgtact agattctact
    21421 gttgaagtct cagttgactc ctcttttgtt gtacttgtag aactttctac tactgatgtc
    21481 gaagtttcag aagaagttgc tgctgttgca ttgttgcttg atccatttct gatccagaaa
    21541 taagcacctg ctggaatagc gatacataag accagtaaaa tcaaaaatag cgaaagaaat
    21601 aatgtatttc ctttcttttg ttgtctctga gaacttcttg atgaaacttc atcatctgta
    21661 tcgtagattg gttgttccca tggttcttgt gtttcgttat tttctgtaga ttgatgtcta
    21721 tcttttctgc tcactgtttt acctcctaaa ctctatcgtg tgtattatac cataggaaaa
    21781 agtgtcttgt cctaaaaaat caatgttcca tgtttttttt aaataaattt agtaaaatcg
    21841 tttcccaatg aagttgttca gtcttagaaa aaagcttcct ctttggcaga agttctgctt
    21901 ttgctccatc aatatcacaa caattctgcg gcttttcgga gagtttttcg ccaaaataag
    21961 ctaagatgaa ttttctgcgg cagttttctt cattaatata gcgaagcatc tgctgcaggc
    22021 ggaattcttt ttctttttcc tgacgtttga gccgttccac ccaattttct ggtttttcag
    22081 actgctggat cagctccatc catttttttt gcagctcatc aaagggagct tgctttgtgg
    22141 catattccaa ataaagctca aaactttgtc gctgttctct agaaagttga ttaaagaaat
    22201 aatggatacg ttcatcccca ctttgataaa aaagaattgc tgcactttct tcctgatctc
    22261 tgcctgctcg accgatttct tgtacatagt tttctagact gtctggcaaa tcataatgga
    22321 ccacataacg gatatcagat ttatcgatcc ccataccaaa tgcattcgta gcaatgagaa
    22381 actgcagctg attttttaca aactgctgtt gaagttgtct tctttgagct gcatcaagac
    22441 caccgtgata gtatccaact gtaaaacgtt ccctgaataa atgatacaat cgttcgactt
    22501 ctttttttgt tgcacaataa atgattgccg ctccttgtgc tctttccatg aagtgttcaa
    22561 gttcttgttc tttttgctgc gttttccgaa caaaaagcga gatattttgc cgattgactg
    22621 attgtctgat ctcttttggt ggtcgatcaa atagtacatg acgtatatct cttgctacta
    22681 aatctgttgc tgtcgccgtt agtgccagtg tgaccgggtt tcctaaaagc tttcggattt
    22741 ttcctaactg ttgatattct ggacggaaat caactcccca ttgagacacg caatgagcct
    22801 catctactac atataacgca atattttgtt ttttcagatg ctctaaaaga gaaggattag
    22861 tcaacatttc gggactcaaa aacaaaaatt tatattgcga tagatgacgg aggacgtatc
    22921 ttctttcaga ttgcgaaagt aaactattga aagcagctac tcgtttctcc cctcgttttt
    22981 gtaattgcgt cacttgatct tccattagag aaagcagcgg tgacacgatg atgaccagac
    23041 cttccattaa ataccccgtc aattgatagc aaaggctttt tcccgttcct gtaggcaaaa
    23101 tagcgagagt atcttctcca tcaagcaacg cctgaataat ttcttcttgg ccgggacgaa
    23161 atgaatcaaa tccaaaatat ttttttaatt catttattaa cattctgtat cgcttccttt
    23221 ctcaatagat agatttgata caaacgaaat tctccataat ctaattgtcc atctacattc
    23281 cattcgctaa agcgccactc tcgcacggaa tccttatttg gttccaaacg ttcgattgtt
    23341 tctgcagaga gaatccactc gtatggaaaa tcatcaaaaa acaaggccca ctcaataaga
    23401 tgatctgtta ctgttcctct ttttaaatga cggattgcca tgatctggtc aattgtctga
    23461 ccattcataa acatctgctt ggtgatcatc atgctttgat tgaaattttg ctgtaataat
    23521 gaatcgatca aagcgtgcca aagcgaatct ggccgttttt cgatttgtgc taacaaaagg
    23581 tggatacatc tgctttgttg cagataaacc tctgactcat cattgttttt tgccagctga
    23641 taaggaagca gccctgttcg ctcatttcca gataattggt tagctaaaaa atctgctgct
    23701 tcttctggta tctccgaaaa cagctgcacc aaactctcat acgcagtatc aattaaaatt
    23761 tcacgtggca attggctttc actaagccat ttttttagtt ggaaagtata aaaaggactt
    23821 gtctctgctg gaagatagtc ttgaataccc gttgccagat tagaaattac ttgaacagca
    23881 aatttaatga gccgccaact agtttcccct gttctgccat agcggtcaaa acgcaaacca
    23941 gtatgttcta tttgatgttc ggacaacata tcagcccctg caggcgttag ctttgcttca
    24001 ttttcattta tttcgatcca accttgctgc gcaatttttt gcaaaatttg ataaaactta
    24061 tcctgcttca attctggaaa actgttatgg ataaacaaaa gttcgtgggt aaatccataa
    24121 ataagtactg aagtcgttcg ttttcccaca aggagttggt aaagagaact aatccgcagc
    24181 ttgtgtcctg atgaaaatag cgataaaata aaaacaatca tattattctc cttaagttag
    24241 gatggttaga aatgtcacta ctatgtaaac ttgtacctga acgctgtatt gcatgcggtc
    24301 tttgtcaaat cgaagcgcca gatattttcg attatgatga agaaggaatc gttttatttg
    24361 cccaagaacc agaggctcat caacagttcg tgatgccgga agaagaaaca tatgtaaaaa
    24421 aagcgtatca aaaatgtcca gttcgagcga ttttattaga aaataaaata taaatataaa
    24481 cgaaacttta aaaacgtaca ctaagttctc taaacttgaa aataagccgg gatctgtttc
    24541 tcttgttccg tgaaatcccg acttattttg aaagattcat ttgctatcta tagttaattg
    24601 actagttttt tcacggaccc tataggaccc atttatatta tttcaacttt ttcagccagt
    24661 tttccaatgt attgacgatc acagtttgct ggtctgcgtt agaaatttct gcctctccat
    24721 ctcctttttg ctcaccatag ctgccaaaac ctccatgatt tccgccattg attgatacat
    24781 actctgttct ttccggtaaa tactgcttag cttcttgata agcttcctga ttcaatactt
    24841 tatcttttgt cgccgtgatc gataaaacag gaacagctga ttctgtcaat gttccttttt
    24901 tgtcgggata acttgccatg aagaataccc cttttaaatg tttgtctgaa tgattcttat
    24961 tgacataacg acttgccatt acgccaccta atgaatggcc accgatcaca taatcggaat
    25021 ttggaaaatc ttctttgacc ttatccgctt tgtttccgcc catcactgct agatcaagtg
    25081 gcattttgac aatagcgact gaaaagccgt tttttgctaa atcttgcgcc caaatactat
    25141 aactttctgg ctctacgaga gcaccaggat aaagaataag taatggagac tttccatgat
    25201 tctttttctc tccttcaaaa aataagtaat cttctttgtt ttttccttct tttaccaaca
    25261 cttgcgcttc tgacgatggt tcgtacgtat tttgctgtat atagtaataa ccggctccac
    25321 cgagtaatcc taaaatgatg actaagccaa ttagtattcg tctaaatatt ttcactttct
    25381 cccacctttt ctatctgagt gacccctttt tcttaatcaa gtatgattat accttaaaaa
    25441 aataagagag aaatgcaaac tttccagcat caaataaaaa aagacggaat atccatttga
    25501 atatccgccc tttttgtatg tttattttta tttagcacat aaacgataca cagcttctgc
    25561 ataaatttcc attgctttat acatactact taattcccat cgttcattcg cctggtgcat
    25621 aaagtcagga gtatcaggga acatcgcacc gaatgccaca cactggttca tcgtacgagc
    25681 aaaagtagcg ccgcctgata caaacggttc agtcatgtct ccagtaatat cgcgatatgt
    25741 ccccaataga tttttcacta actcactgtc tagtggcacg tataatgaat caaggaagtc
    25801 aaattcttca taagtcaacc catatttttc tgctgtttta gttaatttag ctactaaatc
    25861 atctttcttg aatgtaacag ggattctcat atctacaccg atttttgttt gttctggtgt
    25921 gatttccaaa cttgcaaagt tcatcgttag ctcgccggat tgttcatctt ctgttttgcc
    25981 cacgacagct tcaccagttg cattttcttg aactaatttt cctaagaaat tgattgggcc
    26041 aaaatcaaat acttctgaaa gagcaattgc cagacgagaa atagcattca ctccttgtgc
    26101 tgcatctttc gcgtggatac ttttaccaag aacgacgatg ccttcacctt gttcttcgaa
    26161 atcgaattcg tgttttttca atgcttcttt tacttcagaa agcttttctc cagagtaagg
    26221 agctgcatca ggaacaacat tcaacgctcc tccagctttt acagaaaact cgttcgttcc
    26281 tggtcctgtc aagtaagctt gcaataagcc tttttctgca taaattaaag gaaattcagc
    26341 atctggcgca aatccttgcg taattccttc ttctttttcg ttatattttt ctaaacaacg
    26401 ccataaattt tcttcatctg taccaaagat aaaacgaata cgtgtcttga attccacgcc
    26461 agcatcaatc aaagctttga cggcatacat tgccgcaata gaaggacctt tgtcatcttg
    26521 ggatccgcga ccaactaacc agccgtcttt tatagttgga tcgaatggtt ttgtttccca
    26581 attattttcg tcccctgctg gaacaacgtc catatgacac aagataccga ataattcatc
    26641 tccagagcca atctctgaat aaccgtaata gccttctgga tctttaaatg ttctaaatcc
    26701 tagcttttca ctgatttcca atactttatc caaagcttca atcacttttt taccgaaggg
    26761 atgtccttgt cctgaatctg cttcatctag tacggaagga attcggatca attcgctcaa
    26821 tgattctaat gcttgttcat gatgttgttc tgtcacaaat tttttcatga tattatcctc
    26881 ctaatatgtt aaaaagggtc atgtcaaatt cctccgacac aacctcttct tctcaaataa
    26941 tgacgtttta agcctgctcc atcagcaagc agaatgttct taacaaatga tgaaagaaaa
    27001 aacactcact gaatggagct attgacttat ccacgtgctt atcgatagcc gtttatcggt
    27061 ttcttcattc ttatgataga ccttgtacct gtatttgtct aggtttagaa cattgctgct
    27121 acacctagga tcacacagat ggctacaaat aatatcgcaa ttaatttacc ggtaaatttc
    27181 caccacactg aaacatcgaa acgagcaatt gctacagcac ccattacgat agcagatgtt
    27241 ggtgtaatta agtttactaa accagatgca gattgataag cagtgattac taaatcctta
    27301 ccgacgccgg caaattcacc cataggtccc atgattccca ttgttgcagc agctaagccg
    27361 gaagttgaag gaatcaagaa tgacataggg atatagaaaa taaatgtaat aatgatgaat
    27421 acacttgaag ataatgaact taatccttgt tcgccccaat gtaagatggt gtctgtgatt
    27481 aatccattgt tcataaccac ttggatacca cgagcaacag ctactacgat tgctacaccg
    27541 atcaaatcag aagcaccagc taaaaattca gagacaaatt cagactcttt catgccataa
    27601 gtgattgcta ctacgatgga cattactaag aataacatag taatttctgg gaagtaccag
    27661 tcacctaatg gaagcatgct tttacctaga accgtaccta gtaccggaag gtttgttaac
    27721 cagtttgtca atgattcaaa aatcgtgaaa ttcgcattca aggttgtcca aggaatcaaa
    27781 ctgatgatca tgataccaaa agtaataaag aacaatacat taacatgttt ttgttttttc
    27841 gtgattactt cttgacgacc gattgcttca atatcgaaat gcttcatatc ttctttacgt
    27901 tggttgtata ctaatgattt tgttggatct ttttcgatct ttgatgcata acgatacaca
    27961 tatacaatag aaatagctaa caaaataacg aacatcaaga atcgaagacc aataccttca
    28021 cccatcgaaa tcccaacagc ttgtgaagct acgcctgttg caaaaggatt taccgttgaa
    28081 gctagacagc ctacttgaga ccctactaat acgattgcta cagctactac tgtatcaaag
    28141 cctacagcca tcatgactgg gatcagtaaa gcatagaatg gaatcgtttc ttctgccatt
    28201 ccgtaagtag agccgcctaa tgcaaataac agcatcaata ctggaataag cattttttct
    28261 tttcctttga atttcttcac aactgaagca atcccggcat ctagtgcccc tgttttgttg
    28321 ataataccta aaaatccacc aacaaccaaa atgaagaaag agattgggat tgcagcaggt
    28381 gtttctttag ttagctcccc cgctggtaca cctagcatcc cattgattgg cgccatgaaa
    28441 atatcccaca gcccttgagg attttgttgt actcgttcat atgatccagc gacgatgttg
    28501 cctgcatcgt ctacactata gtgcccagct ggtataaacc aagtaagtac agcgattact
    28561 gcaataataa taaataagac cgtatacgct gaaggcattt gaaatttttt cttttttgtt
    28621 tgttccatct tcttcttcct ttcttttccg ttagagacaa caattttggc tctaatgtta
    28681 ttttatcaaa aagagtatca gaacaa
//
LOCUS       DAZUMK010000024        28073 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000024 DAZUMK010000000
VERSION     DAZUMK010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.024"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(72..1121)
                     /gene="recA"
                     /locus_tag="ACG808_001042"
     CDS             complement(72..1121)
                     /gene="recA"
                     /locus_tag="ACG808_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFS5046685.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(1230..2471)
                     /locus_tag="ACG808_001043"
     CDS             complement(1230..2471)
                     /locus_tag="ACG808_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFS5046686.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(2711..3289)
                     /gene="pgsA"
                     /locus_tag="ACG808_001044"
     CDS             complement(2711..3289)
                     /gene="pgsA"
                     /locus_tag="ACG808_001044"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFS5046687.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(3498..4421)
                     /locus_tag="ACG808_001045"
     CDS             complement(3498..4421)
                     /locus_tag="ACG808_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFS5046688.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(5160..6446)
                     /locus_tag="ACG808_001046"
     CDS             complement(5160..6446)
                     /locus_tag="ACG808_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFS5046689.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(6439..7707)
                     /locus_tag="ACG808_001047"
     CDS             complement(6439..7707)
                     /locus_tag="ACG808_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFS5046690.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            7959..8420
                     /gene="mscL"
                     /locus_tag="ACG808_001048"
     CDS             7959..8420
                     /gene="mscL"
                     /locus_tag="ACG808_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFS5046691.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(8479..8925)
                     /locus_tag="ACG808_001049"
     CDS             complement(8479..8925)
                     /locus_tag="ACG808_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5046692.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(9018..9263)
                     /locus_tag="ACG808_001050"
     CDS             complement(9018..9263)
                     /locus_tag="ACG808_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFS5046693.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(9378..9956)
                     /locus_tag="ACG808_001051"
     CDS             complement(9378..9956)
                     /locus_tag="ACG808_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS5046694.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(10033..10563)
                     /locus_tag="ACG808_001052"
     CDS             complement(10033..10563)
                     /locus_tag="ACG808_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFS5046695.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(10567..11427)
                     /gene="rlmB"
                     /locus_tag="ACG808_001053"
     CDS             complement(10567..11427)
                     /gene="rlmB"
                     /locus_tag="ACG808_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFS5046696.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(11420..11815)
                     /locus_tag="ACG808_001054"
     CDS             complement(11420..11815)
                     /locus_tag="ACG808_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFS5046697.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(11812..13224)
                     /gene="cysS"
                     /locus_tag="ACG808_001055"
     CDS             complement(11812..13224)
                     /gene="cysS"
                     /locus_tag="ACG808_001055"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFS5046698.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(13221..13757)
                     /gene="epsC"
                     /locus_tag="ACG808_001056"
     CDS             complement(13221..13757)
                     /gene="epsC"
                     /locus_tag="ACG808_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFS5046699.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(13843..14082)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(14079..15539)
                     /gene="gltX"
                     /locus_tag="ACG808_001057"
     CDS             complement(14079..15539)
                     /gene="gltX"
                     /locus_tag="ACG808_001057"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFS5046700.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(15709..16866)
                     /locus_tag="ACG808_001058"
     CDS             complement(15709..16866)
                     /locus_tag="ACG808_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFS5046701.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(16900..18270)
                     /gene="radA"
                     /locus_tag="ACG808_001059"
     CDS             complement(16900..18270)
                     /gene="radA"
                     /locus_tag="ACG808_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFS5046702.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(18333..18860)
                     /locus_tag="ACG808_001060"
     CDS             complement(18333..18860)
                     /locus_tag="ACG808_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFS5046703.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            19090..19896
                     /gene="proB"
                     /locus_tag="ACG808_001061"
     CDS             19090..19896
                     /gene="proB"
                     /locus_tag="ACG808_001061"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFS5046704.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            19893..21140
                     /locus_tag="ACG808_001062"
     CDS             19893..21140
                     /locus_tag="ACG808_001062"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFS5046705.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            21216..21560
                     /locus_tag="ACG808_001063"
     CDS             21216..21560
                     /locus_tag="ACG808_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046706.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(21677..22003)
                     /locus_tag="ACG808_001064"
     CDS             complement(21677..22003)
                     /locus_tag="ACG808_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046707.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(22618..23820)
                     /locus_tag="ACG808_001065"
     CDS             complement(22618..23820)
                     /locus_tag="ACG808_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5046708.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            23937..24452
                     /locus_tag="ACG808_001066"
     CDS             23937..24452
                     /locus_tag="ACG808_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS5046709.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(24651..25097)
                     /locus_tag="ACG808_001067"
     CDS             complement(24651..25097)
                     /locus_tag="ACG808_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFS5046710.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(25144..26622)
                     /gene="msr(C)"
                     /locus_tag="ACG808_001068"
     CDS             complement(25144..26622)
                     /gene="msr(C)"
                     /locus_tag="ACG808_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFS5046711.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(26976..27605)
                     /locus_tag="ACG808_001069"
     CDS             complement(26976..27605)
                     /locus_tag="ACG808_001069"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFS5046712.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(27589..28053)
                     /locus_tag="ACG808_001070"
     CDS             complement(27589..28053)
                     /locus_tag="ACG808_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS5046713.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
BASE COUNT         7818 a         6086 c         5029 g         9140 t
ORIGIN      
        1 ttcaataaaa aaacggcagg tcaaggagag tacctactct tcagaccagc cgtttttgtt
       61 tatcataatt tttattcttc tactaaaggc aattcttctt gtcctttttc atcttctggg
      121 acagcgactt cttcaccaat accataagct gctctaacta gagcagaaac ttctgccatc
      181 atttcaggat gattagccat atagatttta gcgttttccc gtccttgacc aatccgatct
      241 tctttataag aataccaagc accgctttta tcaacaatgt ctttttctac tgccatgtct
      301 agaagttctc cttcttgtga gatccctaaa ccatacatga catctacttc agcgattttg
      361 aaaggaggtg ctactttgtt ttttacgact ttgatctttg tacggttacc aacaatatct
      421 gtaccttgtt tcaattgttc tgcacgacgt acttccaaac ggatcgttgc gtagaatttc
      481 aatgcgcgtc ctccaggagt gatttctgga ttcccaaaca tcacgcctac tttttcacgg
      541 atttggttga tgaaaatagc gattgttttt gttttgttga tcgaaccaga gagtttacgc
      601 aatgcttgag acatcaaacg tgcttgtaac ccgacatgtg agtcacccat ttcgccgtcg
      661 atttctgctc gtggaactaa agcagcaact gaatcgacta ctacgatatc tacagcccca
      721 cttgatacta aagcatcagc gatctctaga ccttgttctc ctgtgtcagg ctgtgaaaga
      781 agtagttcat cgatattcac acctaatttt tgtgcatatt gcggatctaa cgcatgctca
      841 gcatcaatga aagcggccgt tccgccattt ttttgtactt ctgcaatagc gtgtagtgca
      901 actgttgttt tacctgaact ttcaggtcca tatacttcga tgatacgtcc acgggggtaa
      961 ccgccgacac ctaaagcgac atctaacgca agagagccac ttggaattgt agagatttgt
     1021 tggtcgattt tttcgcccaa tttcataata gagcctttac catagctctt ttcaattttt
     1081 ttcagtgcag catctaaggc tgctttacga tcatctgcca atacgataat cctccttata
     1141 tttacaaatc agagcaacat ttcattatcc ttatcatatc ttttttagtg aaaaaaagca
     1201 agaaaaaacc gaacaaatat tcgcatttat cattttctta aaagctgttt tctcaaaaga
     1261 tcaaatcctt gcataaccgc attatatctg atagcagcac gatctctggc aaagtgatat
     1321 ttttccgaaa ccacgccgtc tttagatgaa atagcgatcc atactgttcc tgcagattga
     1381 ccttctaacg tgtccggtcc agccactccg gtgaaagcta cactgtaatc tgttcttgct
     1441 atctcacgtg cttgaatggc catctgctcc acacattctt tgctgactgt tccatattct
     1501 gctaatagct ccggatcaat ctccagaaaa ccagcttttg tttggagcga gtaggtaacg
     1561 aatccgcctg gaaatacttc agatacacca gcaatgtcgc ctaatgcggc ttggaaggcg
     1621 ccggctgtca aactttccgc tgctgttaca gtctgcttgt tttctttcag tagttccact
     1681 acgacttttg ctagcgaatt atcatcgccg tatccataaa aatattcccc tactcgttct
     1741 tggatttttt cttctaatgc taaaagagct tgattccctg cctgaacatc atttgttttc
     1801 actgtcagtc gtaatgtcac ttcatttggt ttggcatatg gtgcgatcgt tggattgatc
     1861 tgtgtctcaa tcagatcttt tagctcagtg accagtcgcg attcaccaat cccataaaag
     1921 cgcaaaactt tagaaattag tttttcttca gaaggaaaac gttcctctag taaaggacgt
     1981 acttgttcca caaacattgg tttcaactca ttaggcggac cgggaagaag gatatacgca
     2041 tgtgtatctg tttgataaaa agtccctaaa gcaagccctg tacgatttgg tagaggaacc
     2101 ccgccttcga taatctggga ctgttgcaga ttgtttttgg tcatttttcg atgggttgtt
     2161 tcaaaatatg ccaataattt tttgtatcct tccgtatttt gtatgagaga cttaccgagg
     2221 tgagccgcag ttgcttcttt tgtcaggtca tcttctgtcg gtcctaatcc gccacataag
     2281 agtatgagtt cacttcttgt ttcagctaga gcaatcaact cttctaaacg ctgctgatta
     2341 tctcctacaa ctgtttgata ataaacttcg atacctagat cagctagctg ttctgacaag
     2401 aaagtggcat tcgtattcac gacttgtcct agtaaaagtt ctgttccgac agctattatt
     2461 tctgctttca ttacttcccg ctcccttata tagtatacgc tgtattttta tattttttta
     2521 tttaaaaaga gggcaaggag ctgtgacaaa aatcttgtca cagctccttg ttccaaataa
     2581 acagtgttct cctgccaaaa cctcggtcat aagtcacaat atacataaaa catgaaaagc
     2641 tgttttttga aattatccct tgtgactcag agttagacgg caggctcaca acctctccac
     2701 taaacgatac ttacattgat cctttgaaca caccgctgtt tttgatgaag taatccacac
     2761 ctgaatagat agtaaagatc agacaagcat aaagcatgat ttgatctact ggaataccga
     2821 tcaggctgaa tgggatatta ttgataaaca gcaagataat agcaaccatt tgtgtagccg
     2881 tcttgacttt tcccggccaa gctgcagcca tgacttctcc atgttcgaca agtagtaaac
     2941 gcaagcctgt gacggctaat tcacgacaga caatgatcgc tactacccaa ccaggggcct
     3001 ttccttggtc aaccaacatg ataaaagcag tcataactag cattttatct gccaacgggt
     3061 ccgcaaactt gccaaaatta gtgacaaggc ctcgcgcacg ggcaattttt ccatctaacc
     3121 agtctgtgat actagctaaa gcaaaaataa ctgcacctac tagctgtgtg acagccagtg
     3181 tggtgtctcc tacgttaaat tgtccccaat ctaaaggtaa acctacaacc aaaatgaaaa
     3241 tcgggatcat acaaattcta agtacagtta atttatttgg taaattcaca ctatcgactc
     3301 ctttcttccc tattctacgt gactatcagc gaagtttcca ccgatattat ttacttttcc
     3361 aaatgaattt attcaaagga gtatttttat tgaaattgat ctttttgaat taacgaagaa
     3421 ggaccgcgac acagatttgt cacgatcctt cttttttata cctacaagta aacagatgcg
     3481 taaactaatt tatacgatca ttctgcgtat tgaagagtaa ggttcacatt ttttggactc
     3541 tgattgtttt cccctggatt aactggtacg gattctccat tcgctttgat cgttgcattg
     3601 cttgctgcac caatcgtgat ggtcgcattt gttgctgttt ccggtaacac ggtactctgt
     3661 gtttcatttg ctgccagtgt cccttgataa acataggcat tgtctactag cacacctaac
     3721 catacacgat tgacagcagt aaattcaagg gttacaggct tggtagcgtc tgtgatattt
     3781 actgcgatgg cactgccaga atcttctcct gtagctattg ccattttctt ttcttcactt
     3841 gattttgttg ttgattctac tgtagaagac tctttggtct gcgtgctttg ctctgaggaa
     3901 gaggactcct cgactgatcc ttctacttta agcgaagaag ctgtgtctcc gattattgga
     3961 tcagcttgac gatcttgcca agtcatatag cccacgacaa cgacaatcgc caaagcaacc
     4021 aatcctaata aaatgacagg aagacttgtc caaaagcgac ttggatgcga ttcttcctga
     4081 tgcattgctt ttctagaagt acttactgtt tcaggctgcg gacgttcagg gaccgatcct
     4141 gtcaatttgc ttttcccatc aaaaacatcg actaaatggt ctccatcttc tcctacagct
     4201 tcagcgtatt gccgaataaa tgcccgcacg taaaatttac caggcaattg gtcaaaatca
     4261 tcattgtcga ttgcttccaa ataacgtttt tgaatttttg taatctgctg taattcatct
     4321 aaagatattt ttttattcag gcgtgcttgc cgtaattttt ctccgatggt ttcaaaagcc
     4381 actcgttcat cttctccagt ctgtttgatt ctccgaagca tctgctgttg caacgaaaga
     4441 cgttatgttt gggtgctgtg acaaaagtca tgtcacagct ccttttttcc gaataaacat
     4501 tgttctcctg ccaactcctc ggtcacaagt cacaatatcc aataaatata agaagctgtt
     4561 ttttgaactt gttccttgtg actcggagtt aaacggcagg ctcacgacct ctttcttaaa
     4621 cggtgttcaa tcagctgttc ctcggtatta gctcaaaaac cggcaaaata taaggagata
     4681 tttttccgta tgtttctcta atactcaggt caaaacgctg atttcacaac ctcttcctta
     4741 aacggtgttc aaaagcttgc atctgcggta ataagtccaa ccaagttaaa aacattaaga
     4801 agctgttttt acttggctgt tcttattact tgatgcaggg cagctttttc acaacctctt
     4861 ggtacacggt gttccagaag tagcttcttc ggcaataagc cgaaatatca caaaaatttg
     4921 agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct gaccacttct gtcacaacct
     4981 ctttgattac ttcgtttgat tttattttaa tataagttga ttaaaagaga gcatgatttg
     5041 ttttattttc tcactaattt ttccaacaca actaagaata ccataaaagc tgattttgga
     5101 ggaggttcct actacaagtc taaaacgaaa tttaagactt ttcctcataa aatactggtt
     5161 tattttggtc tcatgtaaaa ctcgctgaag gcctcttcac ttataaatgc ttccccagct
     5221 gccagtacgt ctttcatttg gatcgaatcg atgatttcag gaagatcaaa taaagttcga
     5281 tcgccaaata aactttgcgt aaattgattg gcaatatatt cgatcgaatt taatgattgg
     5341 aaatattgtc caagcatttt cttcttcagc aaatcgagat ttgtttctga cacttccgga
     5401 tcatcagcga atccaagaat gatttctttg actttttctg ctgctttctc tggttcgtcc
     5461 gtatctccgc tgaagtccgc aaagtgaaat tcacgatcca agctgaattc aaaaccaaag
     5521 ctgtcatcga taattccttg attgtacatc gctagatagt ttcgagaagt attgcctaaa
     5581 atcatttgga acaacagatt gattgctgtt ttatagcgta acagttctgt tccttcttgc
     5641 ggcaatgtgt ctaatccttt aataccaagt acaaatttat ctcgtgtaat ggctgcttcc
     5701 aatgcacttt gtttgatgat ttcttttgtg ttttcaggga aataacgtac aatttcttgc
     5761 tttggaggga aattttttgc ttcttggttt tcccgaataa gtttcatcaa tttctcaggc
     5821 tccatcttac caactacaaa taaaaccata ttgcttggct gataaaacgt ccgatagcac
     5881 gtatacaagt cttgagctgt gattttgtcg atactttcaa ccgtgcccgc aatatcgata
     5941 tgcaatgggt gtgtaggata caagttattt agtatgccaa aaaacatccg ccagttaggg
     6001 tcgtcttcat acatttggat ttcctgaccg atgattcctt tttccttgtt tacagtttct
     6061 tctgtgaagt aaggtgcttg tacaaaatcg atcagagtcg tcagattctt ttctacctga
     6121 tccgtcgtag aaaacagata gctggttttc gtaaaacttg tgaaagcatt ggcggaagct
     6181 ccttgtttcc caaataattg aaagacatca ccatcttctt tttcaaacaa cttatgttct
     6241 aaaaaatggg caattccatc tggaactttc accttttctt tttcaccata gggaataaat
     6301 tcgttatcga tcgaaccata attcgtactg aacaaaccat atgtcttgtg atagtcattt
     6361 ttaggcaaca aatacacggt caaaccgttt ggcagtactt catgataaag tgtttcattg
     6421 atttgctcat attctgtttt attcatttgc atttcctcct gctaaaaaga agattgcctg
     6481 caattcgatt tgctcagcta cttgttgtat ctctttgatc gttacgctct ccatacgttt
     6541 caaccattct tcatcagata attttgtttc cggcagccag ctgtccaaaa aagaagcttc
     6601 gattgccgct tgcggagagt ctagagaaag cagatattgg tttctcagca ttgcttttgt
     6661 ctgagacagt tccaactctg ttatttcacc atttcgcaaa ctttctaatt gctcgtgaat
     6721 cagatgaagt acttgatttc ggttcttttc atcgataccg gtttggacac tcatgaatcc
     6781 tcggaacgta tccacgctac tagaggcata ataagctaga ctttctttct ctctgacatt
     6841 catgaaaagt ttagagtgag gaaaaccgcc aaataaaccg ttaaagacca ttagcgcaaa
     6901 acgttctggt tcatcgtaat acacatttgt ctgataagct aggttcagct ttgcttgaac
     6961 gattggctca cgtacttgtc gttccttcac gatattcact tgaggttggg tataaaacag
     7021 atctggataa actgtttccc gcgactcaaa tggtaattgc ccaactgctt cagctgcttt
     7081 gtccggatca acatcgccga caacaaaaat gtcgatttga tcatttgcca tcatttcttg
     7141 atagtaggac gctagcgaac tagcagtgat cttgtccaaa tcttcgacta cgcccaaccc
     7201 tgggatcttt tgttccgggc tgttttcaaa ataaagttct tgcaaggcat aggaagcaaa
     7261 agtttgttta tcttccttca aactttccaa atatagacgc agattgttct tttccaagtc
     7321 aaacgtcaac tgatcaaact gatgattttt aatatttgga taaaataaaa cctcttttaa
     7381 aaaatctacc gcttgagaaa aaaggtttgg atcattgaca tattcaccat tcacaaaact
     7441 gatccctgcg tttagccaat gtaaatttcc tttttttcta actgataatc caaagcttgc
     7501 gccataaagc tctgcaagtt gcgcacttaa ctttgtctga tccgaataat tcaaactatt
     7561 tgtttccaac atgctgctga gaagtgaacg tttcgtgatg acttcttgtt gcagacgcgc
     7621 agtaaaacgg ataaaaatcc ggatcgtttt gtattgtttt gttggtaaga catgaagatt
     7681 cacgccttct gccaattgat acaccatatt tatatgctcc ttttcacagt tgattgcccg
     7741 cttactcttt taatgggtca acctgccttt tttacttatt cctctgcttg aaatttataa
     7801 gttcaggcaa ttcgccgttt tttctaaatg atgttacact ttatagtata gcatagatgt
     7861 tttttatcaa aagaaacaga cggctaaaag cgtatattgt ttttttattt tttatttttc
     7921 gatatactaa agacaaataa aagaagggag ggtcattcat gatcaaagaa ttcaaagagt
     7981 tcatcatgcg tggagatgtc ttggatcttg ctgtcggcgt tgttattggt agtgcgttta
     8041 caagcatcgt aaaccaagtg gtgcaaggct tgatcacacc actaataggt tggtttgtcg
     8101 caatgatcac aggtacttct gatttagaag gagcactttc aattcttgac tggtcgccga
     8161 caaaaggcgt cacatttgcg tttggtaacg ttattagtgc tatcattaca tttctgatca
     8221 ctggttttgt cttattctta gttgtcaaag cggcaaaccg tgcgaagaat ttacgctcca
     8281 aagaagagca agtggaagtg gaagaaactc ctactgcaga agattatctt agtgacatac
     8341 gcgatcttct tgccaaacaa gtggcagaag acaatacaca tccagtagag caaacaaatc
     8401 tttccgatac ttctaaataa taaagaatct aaaaaaaaga aagccgattt tcagtgtcgg
     8461 ctttcttttt ttagattctt aacttgctac ttgttccttt ttcatgtcga tatgtggaat
     8521 atcatcttcc aaatacacat ctgagattgc cttgaatcca aacgactcat aaaaagcttg
     8581 taggtattct tgtgcgccga tcacgattgg tttttctgga aaatttttct ggatcatgtt
     8641 aatcgtttct tgaacaatct ttcttcctaa accatcttta cggttgtcct gatgaacaag
     8701 gacacgtcca aaatggatac tctccgcttc ttcatatact cgcgtgtagg caaggatctt
     8761 cccatcagta tcttgaaaaa acgtatgcca agcttctcca tcaatgtcat caacttcttg
     8821 ataaggacac tgttgttcca caacaaaaac tgcgattcgt tctttgacga tcgcgtaaaa
     8881 ctctgctgtt gacaattctt caaatttctt tacctgatat tccactaagc aaacgccccc
     8941 tgctttttta ttcttagcat accgattcgc acacaaaaaa gcaaacgaac cttacgaatc
     9001 tcaggctcgt ttgcttttta aactgcttca gttaaaaatt cgatctcaac tgtccgagta
     9061 agtacatctg aatagctgta agatactcgt tcaaaagaat tttcttcagg atccaaatca
     9121 acaacgaata ctgaaggata ggtttctgtt aaaacgccgc ggcgttctgt ttgacgcttt
     9181 cttcctgtct gagcaaccaa catgattggg ctgccgatac gaccttctaa atctttcttg
     9241 attgaagcta aagttgttgg catacagttc acctctacta tcaactatac cacatatttt
     9301 taaaattttc aagtttacca taaaaaaaaa agaaatgcaa taaaaataaa taagaagctc
     9361 attaagattt ttctaaatta ttcctcatta taaaatttca agtgtttttt tattttgagt
     9421 tttactctcc cgtaagcgct cattacttgc ttgtgatcca ttgctaaaca ttcagccatg
     9481 tctacaaaat caacgccttg catataataa tgaaaaattt tagcttctat ttttgaaaac
     9541 tggatttcgc aattttcgat tgcttctttt aataaaagat gctgaattgc taaaggttct
     9601 tgcaatgtga tctctgctaa aaagtctggt ccttctgtct ctattttttg gtcgattgag
     9661 acttcatctt tttgatcacg acgcttcagc gcgttctgct tcctcaacag agaatagatt
     9721 tgattgctta taacgctttt ataataactt ccaaagctaa cacctttgct tttttcgtaa
     9781 gttctcaatg cctgatataa agcgatcctt gcttcttgtt cccaatcctc agaaacataa
     9841 tttcttaaat aataggtgtt tttcaaacgt agtaaaagtg gaaaatattt ttgatacagg
     9901 gcagcaaatg cttcttcgtc accattacgt gcagcgcgca ataactcctc agacataaaa
     9961 taattcctcc ataaattcgt ttatggagga atcttatcat caatgattca tcgaactgat
    10021 tagaacgata tgttatttat tttttctcat taaatcgtcc aatttttggg acaatttttt
    10081 taactgctct tcattccatg gagaattacg tcggaaatca ataaaatgca tatctttatt
    10141 ttcttgggct attgtttttt ccgttttttt tatcgtctta tataattcat aagcagaagt
    10201 tcgtaatgct ccttgggaaa agatcatcca ctgttctgct aaatcgcttg tcgccactgt
    10261 cacttgtgtc aaacggtcat tcagctgacc agcgattctc tcgatataac tgtcagccgt
    10321 ttcgtcttct ttcgtaaaaa caacatccaa ctggtatttc ttataatttt gttggatgcc
    10381 cggaactagt tgggcatcga agacaacgat gacttccatt ccttcatatt tcgcataatt
    10441 tgataaacga tgtaaaagag cttctcttgc atccgccatt ttgtcctgct ttttaagctg
    10501 gacaagttcc ggccacgaac caatcatgtt gtagccgtct acaatcaaca gctgtttttt
    10561 catggtctac tccccctgac gttgggaaaa agcctgatac ataagcaaac cagcagcaac
    10621 actggcattt aagctctgga cgtgcccggt catcgggatc gtcagcaatt catccatttc
    10681 ttttttcaat cctggactca ttcctttgcc ttcattacca atgactaaag caattgatcc
    10741 tttggcattc catttttgga agtttgtgcc ggtcatatct gtgccaaata cccaaaatcc
    10801 tgtttctttg agctgttgca cagcttgtac caaattcgtc acgcgtgcaa cagggacatg
    10861 ttcgactgcc ccagtggaag tctttgttac gataggtgtg attccaactg ctcggtgttt
    10921 tggtatgatc acaccatcga cgccacttgc gtctgctgtc cgtaaaatcg acccaaaatt
    10981 gtgtggatct tcgatgctat ccaaaatcaa aaagaagggg tcttcttttt tctcttttgt
    11041 tcgttcaatc agttggtcaa gggataaata ttcataaggt gtaatcgcaa gaacgactcc
    11101 ttgatgcacg ccgtgatcgc tcaaagtttc taatttttgt ttaggaaccc atttgactgg
    11161 cacagcttgt tcacgtgcga tttctttcag ttcttctact ttttctcctc gtgaatcttc
    11221 ttgaagaaag agtttattcc ctcgcccttg ttgtagcgct tctactgcgg catggtgccc
    11281 aaagacgaaa ttatcttcta atgcttcttc attttgatca gaaactgact gatttcgtcc
    11341 agtttgcttt ttatttgaat atctattttg ttttttgtct tcaaatccac gtttcgattg
    11401 atgtttgttt gttctttctt tacccatttt tttcaccaac tttctgaata caccagcgga
    11461 tcaattcttc catccgctcg gtttgttttg tcaaatgcag atagcccatg agcgcttcga
    11521 accctgttgc gacccgatac gtcgtaacat cagcattttt tgcgctcgta tggcttttag
    11581 catttcttcc tctgcgataa taaagttctt cttcttctgt caaaagatgt tcttccagca
    11641 tttgctgaat caaaaaggct tgtgcattcg cagaaacata attcgttgcc gcacgatgca
    11701 gcttattggg ttttgtttct ccttgatcaa tcaggtggtc acgaacatag atttcgtaaa
    11761 tcgcatctcc aacataagcc aaggctaaac cgttcaattg tgtgtaatct ctcatgcttc
    11821 tcttctccat cgggttcctt gcggtgtatc ttctaataag atcccttttt ctttcaacaa
    11881 atcacgaatt tcgtcgcttc ggacaaaatc acgattgctt cttgcttgat ttcgttcttc
    11941 gatcaattgt tcaatctctt catctaataa ctcttctgct gtaaataaaa taccgaagat
    12001 cgaaagccat tgcgagaata aagcttctcc ttgtgccaag acagcggcag aaactttcgg
    12061 ctggtctaga taagtgttca gccacttagc aagctcatag accactgtga tcccatttgc
    12121 tgcattaaaa tcatcgtcca tttcttcgat gaatcgctgt tctaaatttt ctaacaaagc
    12181 gattttatcc gcatcatctg ccaaattctc tgaggcagtt tcttggcgga atttcaaatt
    12241 gtcaaacgta ttcttcagtc gttgtaaatt ggtggctgct tctttcatcg tcgtttcact
    12301 gtagcggatc ggacgacggt attgtgtggt cgccatgaag aaacgcaaga cttgtggatc
    12361 aacgtttttg atcatttcat gtacagtgat gaaattccct agagatttac tcattttttc
    12421 atcgtcttcg ccaatcgtta catagccatt gtgcatccag taattagcga attttttccc
    12481 tgttttcgct tcactttgtg cgatttcatt ttcatgatga ggaaattcta aatcttgccc
    12541 gccaccatgg atatcgatcg tttctcctaa atgttttgtt gccataacgg aacattcaat
    12601 atgccaaccg ggacgtcctt tgccccatgg tgaatcccaa gaaatctcgc caggttttgc
    12661 ttgtttccat aaagcgaaat ctaatgggtc ttctttttgc tgctgttctt cccctgtacg
    12721 ctgacttgct ccgacttcca gttcatcgat ggattggtca cttaatttcc cataattttc
    12781 aaatttacgg gtacggtaat acacatcacc tgaagattca taagcatagc ctttttcaac
    12841 caacacttga ataaatgcta aaatatctgg catatgatcc atgactcttg gatgcagtgt
    12901 agcaggttcg acattcaacg ctttcgtatc ttcttcaaat gctcggatga aacgatcagc
    12961 aacttctggt gctgtaatac ctaattcgtt tgctgtacgg atgattttat cgtctacatc
    13021 cgtgaaattc gatacgaaat cgactttata gcctcgatat tcaaaatagc gtcgaattgt
    13081 atcgaaagca atggtgctac gagcatttcc gatatggata taattataca cagtcggtcc
    13141 gcagacatac ataccgacat gtcccttttg tatagggatg aattcttctt tttttctggt
    13201 caatgtattg tagattttta tcattctgat ctcctcactt tttctccttt gattcgaacg
    13261 acttttgctg gtactcctac cgctgtagcg tcgtctggaa catctttcaa cacaacggca
    13321 cctgcaccga ttttggcgtt ttttccgatc gttaccggtc ctaatatctg cgcattcgca
    13381 gagagcatgg ctccttgttt aacagttggg tgtcttttac ccgtttcttt tcctgtgccg
    13441 cctaatgtta cgccatggaa taaaatcaca tcgtcctcga tttctgcggt ttcgccaatg
    13501 acgattccca ttccatggtc gatgaacacg ccagttccaa tcgttgctcc tggatggatc
    13561 tcaacaccag ttaggaaacg ccagaattga gcattgatct tcgccaataa aaagagccgg
    13621 tgctgatata aaaaatgaga gaaacgatgc caaaacaagg catgaaaacc agggtatgtc
    13681 aaaacgatct cagctgtcga acgtgcagcc ggatcgtttc tctttgctgc atcaatcgct
    13741 cgggtaaacc aactcatgac tttccactcc tttcgtaaga attttatgta ggaaatcatc
    13801 ttcttataaa gaaaagaagc gccttttaaa taagtttcct tatcaaaaga cgctttccgc
    13861 gtggttccac ttttcttcat aatcaatgat tatctctgac agctaacgcc tgttatacgt
    13921 tcttggctac tttgtttcct ataaaacagt ttttcctgtt ttctgtgttt cgccaagacc
    13981 actcccagag gcatttcggc aaccattcat tgataccttg caccacccgg tatctctctt
    14041 gtaatgatca gctgcttact tttttctgtt catcgtgttt agttcagtac ttgatttaaa
    14101 tgtgctaatg atttttcacg accaagcaat tcgatcgttt caggcaattc tggaccatgc
    14161 atttgtcctg atacagctac acggattggc atgaaaagat tttttccttt tacgccagtt
    14221 tctttttgaa cagctttgat ggcagctttg acagttggag catctactac ttccatttgt
    14281 tctagctgtg ctttgaatgc gtggagaaca gtaggaactg tttcaccagc aagtacttct
    14341 tttgctgctt cattcaatac aggatgttca ttgaaaaata gcgtagacag atcaacgatt
    14401 tctgcagcat agctcatttg aggttgatac aggctaacga tcttcttcac ccattcgatt
    14461 gtcttttcat ctgggttttc tgctacacgt ccatcagcga ttagataagg cagacacatg
    14521 tcagtaaatg catctagatc cattgctttg atgtaatggt tgtttaccca ttgcagtttt
    14581 ttcgcatcga aagctgcggg tgatttactt aggcgttttg gatcgaacat tttgatcaat
    14641 tcttggtgtt cgaagatttc gtcttcgccc acaggtgacc agcccaataa tgcaataaag
    14701 ttgaacattg catctggtag atagcctaat tcgcgatatt gttcgataaa ttgcaagatt
    14761 gattcgtcac gtttgcttag ttttttacct gtttcactgt tgatgatcaa tgtcatatgt
    14821 ccgaaggttg gtggtgtcca gccaaatgct tcatagatca tcagttgttt tggtgtattg
    14881 gcaatatggt catctccacg taaaacgtga gtgattttca tcaaatgatc atcgaccgca
    14941 acagcaaaat tataagttgg cataccatcg cgtttttgga tgacaaagtc gccaccgata
    15001 ttatcggatt cgaacgaaat agcgcctttc accatatctg taaatgcgta ttctgtattg
    15061 cgaggcacac ggaaacgtac aacagattct agaccttgtg cttctttttc tgcttgttct
    15121 tcaggtgtca agttcgcaca tgttcctgcg taatgaggca tttctcctct tgcgcgttgt
    15181 gcttcccgtt ctgcttctaa ttcttcttct gtgcaatagc atttgtatgc tcgattgcta
    15241 gcaagcagct gatcaatcag cggttgatag atttctttac gttcagattg acggtaaggg
    15301 ccgtattctc caggattttc aggagattca tcccaattca ttcccaacca agcaaggttt
    15361 tccaactggc ttttttcgcc atcttcgata ttacgttttt ggtcagtatc ctcaatacga
    15421 ataataaagt ctccatcatt gtggcgggca aacaaataat tgaataatgc agttcttgca
    15481 tttccaatat gcaggtgccc agttggactt ggtgcatagc gtacgcgtac tttcgtcatt
    15541 ttcttcctct tctttcaaac tagactttga ctaacagcaa aaattccgtc agttacggct
    15601 tctcaaatat catttattta aagccactca gcacaaattt taccttttct aaggtaaata
    15661 gtaaagccaa ctggttgaat ttccttacca gttggctgaa tttctttttt attttgattg
    15721 tttagtcttt ttctctgtct cttttttatt gtcgattcct cgtcctgcat gtgcaggttt
    15781 agcaaagatc atgcgtccag cagctgtttg aagggcactt gttacaacga cttcgatacg
    15841 ttcattcatg taatgctgtc catcttctac gacaaccatc gtgccgtcat caaggtaggc
    15901 tactccttgt tggcgttctg tcccggcttt tactactaga acattcatcg tttcaccagg
    15961 gatgacgact ggttttacgg catttgccaa cgcattgata ttaagaacag gtacattttg
    16021 gaattcagag actttattca agttatagtc attggttaca atgacaccat ctaaaagctt
    16081 agccaatttg atcaatttgc tgtctacttc actgatatct tcaaagtctc catcgtacat
    16141 ttcgacagat ataccctctt ctttttgcaa ggcatttagg atatctaatc cgcgacgacc
    16201 acgcacacgt ttcaaactat cccctgagtc cgcgatgtat tgtagttcgt aaagaacaaa
    16261 attagggatc atcaaggttc cttctaagaa tccagtcttt gtaatatcat aaatccgacc
    16321 gtcaatgatc acgcttgtat ccaagatttt atatttatgg aaattttctc ctgattttcg
    16381 ttctaacagt tgactctcaa cttgactagc tgtttcttgc tcgatttttt tgcttcgtga
    16441 accaaaaact ttcttccatt cttcgattcg tgtcgttccc attctcaatc ccagatagcc
    16501 taagatgatc atcagtaaaa tcggcaatac actattgacg aatggaattt ccatattgta
    16561 aagaggggtc gagatcacaa cacctaaagt caatccgatg atcgcaccga tcgcaccgaa
    16621 gagaagataa gtcaagctga cctcacttag tttttgttct acttgtttga taatagctga
    16681 gatatgtttg gccaacagca aagataataa aaagaaaata agtgcgccta acaggctgtt
    16741 ggtaaatgta ttattcagcc atccgtttga ctgttgaccg attgcctgcc aagctgaggg
    16801 taaaaatgaa atacccaagc ttgcgccgac aatgatcatc agcaaagtaa tgacacgttt
    16861 ttgcatcgtt tttcctccgt ttcgacaatc atttattagt tagcggaaca cacgcttcaa
    16921 cgtttcagcc aaagttgcta ctccaactat ctcgattcct tttggcgctt cccagttacc
    16981 taagttgttt ttaggtaggt atacttttgt gaagccgagt ttttggactt ctttgactcg
    17041 ttgttcaata tgactgacac gtctgatttc tccagtcagt ccaatttcac cgatgaaaca
    17101 ttccgttggc tgtgttcctt tttctttata gcttgaagca atactgacag cgatagctaa
    17161 atcaatcgct ggttcattta attttacccc acctgcagct tttaagtaag catcttgatt
    17221 ctgaagaagc aacccagctc ttttttccag cactgccatg atcagagata cccgattgaa
    17281 atcaagacct gttgttgttc gtttggcatt tccgaacata gtaggtgtga ctaatgcttg
    17341 gatctctacc aaaattggac gtgtcccttc cattgccaca acgatagcag agccagttgc
    17401 accgtctagg cgttcttcta agaaaatctg tgaaggatta gcgacttctt ccaatccatg
    17461 ctcatgcatt tcaaaaatcc caatctcatt cgttgagcca aatcggtttt ttactgctcg
    17521 caaaatccga aaactatgat gttgttcacc ttcaaaatat aacaccgtat ccaccatatg
    17581 ttctaacatt cttgggccgg cgatcgagcc ttctttggta acatgaccga cgataaaaat
    17641 ggctatccca tttgttttag cggttttgag tagttctgca gtggtttctc ttacttggct
    17701 gacacttcct gccacacttg tgatgtctgg ctgcgtcatc gtttggatgg aatcaatgat
    17761 aacataatca ggggagatat gttcgatggc acgactgatt tcgttcatat ctgtttcagc
    17821 gtatagataa aattctgtgt tgatacttcc gaggcgttcc gcgcgtaatt ttatctgctc
    17881 tgcgctttct tcgccggata catataagac ttttccgcct atggctgcta attgctgaga
    17941 aacttgtaac agcaaagtgg atttcccgat acctggatcg ccaccaatca agaccataga
    18001 ccctggcacc acacctccgc ctaacacacg gttcagttct tctaattttg ttttgatccg
    18061 aggttctttt ttagggacga cgtctgcgat ttttgttggt tttgcttttt ctcctgtcaa
    18121 acttgtccga gttctgcggt ccgttgtatc ttgttcgatt tcttcgacca tcgtattcca
    18181 ttttccgcaa tttgggcagc gtcccaagaa cttaggcgac acatacccgc aattttggca
    18241 aacaaattgt acttttgatt tttttgccat tgtaccgcct ccttttatcc taatcatgag
    18301 acattaaatc aagcttaagt atctttaacg ttttatttac tggaagaacc aaatccgcct
    18361 tgacgttgag cagtcgtttg atcttgatct gcttttaaaa acggcaaaaa gatcccttgg
    18421 ccgatacgct cgcctttttt gatcactaca tccttgaaac caaagttggt gaattggaac
    18481 atgatatggc cttcatttgc ctcattattg taataatcac tgtcaatcac acctactccg
    18541 tttgtcaaca ctaaaaaccg cttcaacgga ttgcttgaac ggttagcaag ctgcaaaaat
    18601 tcatcttctc ccatgtaagc tttgattcca gtaggcacga gggttggttt caattgtttt
    18661 tcattttctg atacatcttt tttcaatcca tctgcgactg tttccaaccc ctttttccag
    18721 cctgctttcc agatacttgg aatcacgacc tcttctgctg cttcaaaatc atagccagca
    18781 gcatgatgtg tagcgcgctg cggtagattg atattttgat ctttatattt agaaataact
    18841 tcaaatcctc tttttctcac cggttttcct ccctaaataa actactccta ttctaacata
    18901 tccgaggctg taaaagactt tctttacaaa ttcttcaaga aattctgcta tcttaaagta
    18961 aaccattaat tcgttctttt tccaaacgac tgcaagttgt acaaaaaaac tattttttac
    19021 atgactttac tcgtcaaaac aagtagaata gaacgaaatt aatgagtgga tatttgattg
    19081 aggtgtcaca tgcgtaaaga aattaaaaat gcaaaacgaa ttattgtcaa agtaggaaca
    19141 agtacattga tttatcccaa tggaaatatc aatcttgcgg caatcgatca gttagctttt
    19201 gtcctttctg atcttagaaa tcggggaaag gaagtgatct tagtctcatc aggagctgta
    19261 ggcgttggta tgcataaatt acatttgtca gaacgtccga ctttgattcc tgaacagcaa
    19321 gctgcggcgg ctgttggtca agccgaactg atgaatatct ataatcaaag attcagtgta
    19381 tataatcaac agacggcaca gatcctcgtg acaagagacg tggtagatta tcctgaaagt
    19441 cgtaaaaatg tgaggaatac attggaacag cttttgatta tggacacgat ccctatcgtg
    19501 aacgaaaacg atactgtagc agtggatgaa ttagatcatc tgacaaaatt tggcgataat
    19561 gatcagctgt cagccatcat ggcaaaactt gtcgcggcag accttttgat cgtcctttct
    19621 gatatcgacg ggttttattc agataatccg actacaaatc ccgaggctca gatgtatcgc
    19681 tatatcgata aaatcgatga aaagattctg gcacaagcag gtggtgctgg tagcacattt
    19741 ggaacaggcg gtatgcagag taaattaaaa gccgcttcta ggatatttga agcaaacagc
    19801 gcaatgattt tagcaaatgg aaaaaatccg gcaattattt ttgatattct tgcaggaaaa
    19861 gaagtaggta ctttatttaa ggaggacttg aaatgactga cttgatgcaa ttaggacaac
    19921 aggcaaaaga tagcgcgcaa caactagcac ttatgcctac aaaccgcaaa aatgagctac
    19981 ttgttcaaat ggcacaaacc atcaaaaaga atcaacaagc gatcattgaa gcaaatcata
    20041 aagacttaga aaaagcagtc gaaaacaata tcagtgaaac gatgcaagac cgcttgcagc
    20101 tgacacctga gcgaatcaat gccatggcta cagagatcga aaaaattgct tcgttagacg
    20161 atcctattgg aaaagtagat gagatgtgga cgaacaccga cggattgaga atcggcaaaa
    20221 agcgcgtgcc actaggcgtg atcggcatca tttacgaatc tcgtccaaat gtcacgacag
    20281 atgcagctag tctagcattc aaatcaggga atgccgttat ccttcgcggt ggcaaagaag
    20341 cattcttctc gaatcaatta ttagtccaat tattgcagca agtactggtc agtgctggcg
    20401 aatctcctta tgccatccag tttgtcgatg atacttctca tgaaactgca ggacagttga
    20461 tgcgattgac agaatactta gatgtgctga ttcctcgagg cggagcaaaa ttaatccaac
    20521 gagtcaaaga acaggcaacg gtacctatca ttgaaacagg tacaggaaat aatcacgtat
    20581 atatcgataa agaagcacag ttatctatgg cagtgaacat catcgtcaat gcaaaagctt
    20641 cccgtccatc tgtctgtaac gcggcagaaa cattgctgat ccattctgag atcgcacctt
    20701 ttttcttgcc agctattgaa aaagaactgg tagaacacgg ggtctcttta cgtgcggatg
    20761 ctcgtgcgtt agaatatcta gagacggctg ctttagcaga agacacagat tgggatactg
    20821 aatatctaga ttatatcctt gctgtcaaag tggtcgattc tttggatgaa gcaattgcac
    20881 atatcaatcg gcataatact aaacattcag aaacgatcgt gacagatagt tatgcggcta
    20941 gtcaacgttt cttaaatgaa gtggacgcgg cggctgtcta tgtcaatgcc tccactcgtt
    21001 ttacagacgg atcggtattt ggttttggtg cagagattgg tatctctact caaaagttgc
    21061 atgcccgtgg tccaatgggg ttaaatgaat taacttccac aaaatatatc atttatggcg
    21121 atggacaaat ccgcgaataa tgtttcgtgt gaaacattat tcccgccttt tgcctttttt
    21181 ctgtaaaggt tctatccaac tagaaaggtg tcaatatgtt tgaaatttta atgatttttt
    21241 ttatttacct tatttctctg aatatcgctg cttttctggg agtcggtatt ttatcccttt
    21301 tctttcaatt caaaaaacga agcataggga gtcaacggga aaaatggtcg cagtattttg
    21361 acaaaatcgg gcaaaaaggg ctggttacac gcttgcacat tagttacatg gttgcattat
    21421 gcttattagc gacaatcaat tactattctt ttttcgatca ttcaatcgct tatacgatta
    21481 cgctactgat tgctgggatt tttcacttat cttataaata tcagctgaat aaaaaccact
    21541 tgaatcggac tttccgctaa aaaatatccg tacgataagt agagtgcctt gaagatcggc
    21601 aaaaaactgc cggacttttt agaggctgtg acaaagattt tgtcacagcc tctttttcca
    21661 tataaacggc gttcgcctac cagctgcctg gtaaatctgt ttcctcttcg ttaagaagcg
    21721 aaaatccgtc aaatgtttcg ccatcataat aacgaagcag ctcatccatg gacaagttca
    21781 tcgttttttc tttttcttct tccgctgatt cagccagtag ctgttcccga tacacctgat
    21841 acataccagc tattttttgc tgcatcgctt ttctagtttc accttccgca ttgattattg
    21901 gcaattcttt gatatagcca cgataaatca accgatcatc tgaaaattca atgggtaatt
    21961 caacgagtgt caacccgact tcctgaaaat attcttccag catctattat ccccccattc
    22021 tttttcttaa atgaatttta gcttacctac tgaaaatttt ccagtttcat tattttgaga
    22081 aaaaaaggga atgtgacaaa aatcgtgtca catccccttt ttccgaataa atggtgttct
    22141 tctgccaact cctcaatcat aagtcacaat attcaacaaa catgagaaac tgttttttga
    22201 aattgttcct tgtgattcag agttaaacga cagtctcaca acctcttggt aaacggcgtt
    22261 caatcagctg accctcggta ttagctcaaa aaacggtaaa atatgagaag ctatttttcc
    22321 gttttgtttc tctaatactt aggataaaac gctgatttta caacctctgt taaacggtgt
    22381 tcaatcagct gtccttcggt attagctcaa aaaacggcaa aatatgagga actatttttc
    22441 cgtttgtttc tctaatactc aggacaaaac gctgatttta caacctcctg ataaacggtg
    22501 ttcaaaagct tgcatctgcg gtaataagcc caaccaaacg aaaaatatga gaagctattt
    22561 ttgtttggct gttcttatga cttgatgcag gacagctttt tcacaacctc tgatttctta
    22621 ttcttcttca tttgtagcaa aataattacg gtgataaatg atacaacata cactgataag
    22681 aacaatggca cctgcaaaat aaaacgcata gctttcacca atatgttcac caatcacacc
    22741 tgcaataaat ggtgctaaag ctccaccgaa aaagcggaca aaactatagc tggacgaagc
    22801 aacgtttcgt tgcagacctg gtatttccat agcaatcgtc gtcaacagtg tattgataat
    22861 tccttggaaa aatcctgcta acacaatgct gatcggtaca aggactggca tagaagttcc
    22921 agctcctaat aacagcaaac aaataatgaa taagacaaat gctgctaaaa ttgtccgata
    22981 agtcgtaaac ttccgctcaa gagccggtgc aacgaaaatc gaagcaatag ccagaagcac
    23041 accccagcca aaaaatacaa aaccgacttc taattcagaa tagccaatca ataaaaatgg
    23101 cgcatacgca agtaaagtaa agaatccgaa attatataac aatgcgatta gtccaactga
    23161 acgaagttta tgattgctta acgctttgac accttcccaa aggcctactt ttctctttgg
    23221 tttctcagtt tcaggtaaaa gtaccgctag ggctataaaa ccgcaaaaca tcaaaaatgc
    23281 taccccaaaa aatggataac gccatgaacc actgcccaag accccaccga ctagagggcc
    23341 aacagccatt ccgcagccaa gtgctgcttc atacatcatg attgcttttt ctgtgttacc
    23401 aactaaaata ctgacgatcg ttgctaaagc agtagagaca aataaagcat tcccgacacc
    23461 ccagccggca cgtaacccaa tcagcattcc gatattccca gataatccgc caagaccagc
    23521 gaatacaacg atgataaaca atcctgtcat caatgttttt ttcaaaccga tccgactaga
    23581 taagaaacct gtaaatagca tcaccacacc agtgactaac atgtaactag taaataacag
    23641 ggtcgtttca gctggcgtag cgtttaactg tgcagcaatc gtttttaaga ttgggtctac
    23701 tagacccact cccataaaag cgatcataca ggtagctgct acaactagcg ctgaactttt
    23761 cccctttgtt tcttcatttg tttgttcttg tatatctatt tgattctcca atatttccat
    23821 ccctttctct ctattagcat catacatgat ttattttaca tgtcaatatg cacatgtaaa
    23881 acgtggaaac tttactgtga tataataaag aaaataagac gagaggtagg atttttatga
    23941 atactttagc ctatatatta ttatgcgtgc ttttatccaa accacgttct gggtatgaat
    24001 tgaagcagct gatcacaatt ttttgggaag cccatcacag tcagatctat acaacgcttg
    24061 ccaagttaga ccagcagggt tatgtgaccg ttttggacaa agacgagcac tctcaaaaaa
    24121 agatctacca cttgactgat gaaggacgtg ctcttgtaga ggaatggatc aaagaggaaa
    24181 ctcccgctcc tacgcaaaaa gatgaatttc tagcaaaaat ctatgctttt tcgacgctcg
    24241 ataaaacaac agctactgga ctgctattca ctagagaaca acagctgaac aaaatcatgc
    24301 aaaaaaatca aaaaaaattg aatgacctct cgctagacaa gaaacaagaa tttggacgtt
    24361 atgttgtgat ccagcgcaga attatgcttt gtcagcagga actccaatgg tgtcagcaag
    24421 taagagaaca aatgcagcag ttttttgagt aagggataaa cagaaccttt ctagaaatat
    24481 ctattgaaga gcaaattatc ttcaataaaa gagcaataca gttggaaaag aaaaagcgat
    24541 ccgaacgaca ggccaaaacc tatcgttcgg atcactattt ttctagaact gcattcaaga
    24601 gactatgaca aaaagcatgc caaggccttt tttcaaataa cagtgttcca tcattcatta
    24661 ctttgaattg cttttagcac acttcttgaa agcgggccaa cgatgatcaa ctgcaacggt
    24721 aaagcggcaa tcacattcat tttccaagca ttcatataag cagagaataa ttgatcaggt
    24781 attccttgtt ccatgatcac accgaataaa gacatacatg tcaccattcc taaaaccatc
    24841 aagcatgaaa tagttaagat catttggacc ttgctttctg gatttactgg tagtttaaag
    24901 gcaatttttt tcgccagcac accgatgatg aagacatcca aaatgaaagc caccacgaat
    24961 ccaggcacta gaccaatcaa caaacttgat aaatgaagat cttgatgcag ccataaatta
    25021 tacgcgctca tccccaagac catcaaaaaa cacatcattg ttgtaaaaat tattccttct
    25081 tttttatttg taggcaatct gtaccactcc tttaaatttg tcacgaaaat catcgtagca
    25141 catttaaaaa tttctcgtaa gtactttttt tattttctgc ccttttattt cataaacttc
    25201 atcagccgta cgctcgacaa agtaagaatc atgagaagta aacaaaatcg ttcccggata
    25261 tatttgcatt agcttctcta aagcttcgat cgttgccata tcaataaaat tagtcggttc
    25321 atctaacagc aacacattac ttggcttagt aaaaagcaaa gcaatcgtca gacgggtcgc
    25381 ttctccccca cttaatgtac ataacggttt cgtcaaagtt tcctgagcaa accctaagtg
    25441 attcagcaaa ctacggacaa gcgattctga aaactctgtt tgtctcatga caaaacgaat
    25501 gatggattct tcagatgtca tttgataacc cttttgctga tatacttgaa agcttacttt
    25561 cggagagaga aggattcctt ctccttgttt gcggatatgt tctaataagg ttgtcttacc
    25621 tgaaccattt tcaccgacaa tcgcgatttt tttacctaaa gaaaactgaa aatcaccatt
    25681 taccagtaat gttctcccac ttctttccaa ttggacattt tgtcccatga tgggatagcg
    25741 gctgtggagt tcaagagatt ttggtactgg aaaacggatt tgtttcatct gctgcggctt
    25801 ggttgtttct cctatttttt ggagtcttct ctctaatgtt ttcgcattct tttggatggc
    25861 tttttgtaca ctgtcttttt gtttagagga agacaaccga tccagtctga tttgttgctt
    25921 tttcttttta ctggacactt tttccaactt ttccgcttgt tttttctttt cttggatcga
    25981 tttttccaat cgttttttct ctttcatgat cgactctgct tctcgtagct gagtcttttt
    26041 ctccaattcc ttttgagtga gataggcact ataattcccc gaaaactctc ggatttctcc
    26101 gtcttttact tcccagattt tcgatgctag ctcatctaga aaatagcgat catgactaac
    26161 aaaaaccagc gtaccataat aataacgaag ctcttcaatc agtcgatcaa tactttttct
    26221 atctagatgg gttgttggct cgtctagaag catcccttgt tcataagaag aaagaagacg
    26281 tatcaactcg atctttcgtt cttcccctcc gctccatttc cgctctgaag tttcttccag
    26341 tcttaactgg aaatgactgg ctaattccaa atttaggtcc tttgcttcag cgagataggt
    26401 taattgaggc aggtagttga aggtgatctc tgtttggatt ttcccatgat cgggaaaaag
    26461 ttcaccggct attagtttga gcaaagtcga tttaccgaca ccattttctc ctatgatccc
    26521 tatacgatct tgctgataga cacgaagaga atcaatagat agttctagct ggttgccaaa
    26581 gctgacttgt agatttgtta tatttactgc taaattttcc atatgtttta cactcctatt
    26641 tcatgtaaaa caacaattat cgatactaaa aaagagcaga aaaatctgtt tgcaaaaaaa
    26701 ctgcatcaca tcttttccta ctctgaacga aataactatg actaaatcac caaatctgtc
    26761 atagctttca cctattctac tgtcgttcga aaaaatggac atactccatc catgaaattg
    26821 ttgtttatac atgtcttttg aatttattta gttgttattc aaaagttcga aacgtaattt
    26881 catcgatgca gtcatttttc cgacctcctt ttttattagg ttaccacaca cgattcaaaa
    26941 aaagcaatag ctactgaata gtaactgaaa accttttatt cgaacatagt gatcttttct
    27001 ggtgctaaac gaacagactc gtaaccttct tctaccgctt ttcctattga taaaataata
    27061 actggaacat aacggtcttt atccaaacca aatgcctctg ccaactgatc tgcttcgaat
    27121 ccaccaattg gatttgtttc atagccatga tcacgcgcaa ctaacatgaa ttgcatagcc
    27181 gctaagctag aatcgacttt gactacatcg ttcatttctt ctcttgaaaa actcttgtaa
    27241 taaggaatga tcgcagctaa ttgttgatct ctgacttctt ttggcatctt ccctgattcg
    27301 taagcttggt tgtagatttc ttcgccatat tcgtaacatt gcatatcgcc aaaaatcatg
    27361 accattgcag aagaagtatc attttgtctc gtattgaaac ggatcaaagg acgcaagatt
    27421 tcttttgctt cctcgctttc agcgaccaca aagcgccatg gtttcatatt gacagaagaa
    27481 ggtgcagtcg tcgcttcttt gatcatttcc agcatttctt cgtgtgggat tttgtatgtt
    27541 tcatcatata cgcgaacgga tcttctacca gtcataattg ttgaaaaatc atttttttgt
    27601 tccattatta ttaacctctt tcgtttctgt tgttatattg caaagttggg tattcaattt
    27661 atctaaaagt gttagaaatt gtttcatttc tacatcgctg aaagccgggg atagcacgga
    27721 acctgtatct ttatggtcca tttcacaatg cttgattttc ttctgggctt tttctgttag
    27781 cgctacatac acttcccggt tgttctgagg atttctctga cgcgaaacat agccatttgc
    27841 ttccaatacc ttcagatgac gagtaactgc tccttgatca atctgcaact tttcttgcaa
    27901 actgctttgt agcaatggat tttcttcttt cagtctcatc atgatctcgt atctagtaag
    27961 acttataccg atattttctt caaagatctg gacgattttt tgatcggcta atttgatttg
    28021 atataaaagg tgactaatct cttctaaaga cataatttcc tcctttctaa taa
//
LOCUS       DAZUMK010000025        27961 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000025 DAZUMK010000000
VERSION     DAZUMK010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.025"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            25..696
                     /locus_tag="ACG808_001071"
     CDS             25..696
                     /locus_tag="ACG808_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS5046714.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            755..1195
                     /locus_tag="ACG808_001072"
     CDS             755..1195
                     /locus_tag="ACG808_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046715.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1207..1875
                     /locus_tag="ACG808_001073"
     CDS             1207..1875
                     /locus_tag="ACG808_001073"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFS5046716.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1939..2562
                     /locus_tag="ACG808_001074"
     CDS             1939..2562
                     /locus_tag="ACG808_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFS5046717.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2618..3004
                     /locus_tag="ACG808_001075"
     CDS             2618..3004
                     /locus_tag="ACG808_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFS5046718.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3217..4608
                     /locus_tag="ACG808_001076"
     CDS             3217..4608
                     /locus_tag="ACG808_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFS5046719.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            5219..5587
                     /locus_tag="ACG808_001077"
     CDS             5219..5587
                     /locus_tag="ACG808_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFS5046720.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5767..6708
                     /locus_tag="ACG808_001078"
     CDS             5767..6708
                     /locus_tag="ACG808_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFS5046721.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6777..7508
                     /locus_tag="ACG808_001079"
     CDS             6777..7508
                     /locus_tag="ACG808_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS5046722.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7652..9832
                     /locus_tag="ACG808_001080"
     CDS             7652..9832
                     /locus_tag="ACG808_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFS5046723.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9852..10334
                     /locus_tag="ACG808_001081"
     CDS             9852..10334
                     /locus_tag="ACG808_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFS5046724.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHQAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            10350..11111
                     /locus_tag="ACG808_001082"
     CDS             10350..11111
                     /locus_tag="ACG808_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFS5046725.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            11242..12576
                     /gene="murC"
                     /locus_tag="ACG808_001083"
     CDS             11242..12576
                     /gene="murC"
                     /locus_tag="ACG808_001083"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFS5046726.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12883..13368
                     /locus_tag="ACG808_001084"
     CDS             12883..13368
                     /locus_tag="ACG808_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFS5046727.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            13361..14044
                     /locus_tag="ACG808_001085"
     CDS             13361..14044
                     /locus_tag="ACG808_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFS5046728.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGIILAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            14170..16815
                     /gene="polA"
                     /locus_tag="ACG808_001086"
     CDS             14170..16815
                     /gene="polA"
                     /locus_tag="ACG808_001086"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFS5046729.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16860..17696
                     /gene="mutM"
                     /locus_tag="ACG808_001087"
     CDS             16860..17696
                     /gene="mutM"
                     /locus_tag="ACG808_001087"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFS5046730.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17660..18289
                     /gene="coaE"
                     /locus_tag="ACG808_001088"
     CDS             17660..18289
                     /gene="coaE"
                     /locus_tag="ACG808_001088"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFS5046731.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            18341..18673
                     /locus_tag="ACG808_001089"
     CDS             18341..18673
                     /locus_tag="ACG808_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFS5046732.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            18985..19476
                     /gene="nrdR"
                     /locus_tag="ACG808_001090"
     CDS             18985..19476
                     /gene="nrdR"
                     /locus_tag="ACG808_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFS5046733.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19499..20872
                     /locus_tag="ACG808_001091"
     CDS             19499..20872
                     /locus_tag="ACG808_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFS5046734.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20872..21798
                     /gene="dnaI"
                     /locus_tag="ACG808_001092"
     CDS             20872..21798
                     /gene="dnaI"
                     /locus_tag="ACG808_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFS5046735.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21927..22367
                     /locus_tag="ACG808_001093"
     CDS             21927..22367
                     /locus_tag="ACG808_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFS5046736.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            22511..23212
                     /locus_tag="ACG808_001094"
     CDS             22511..23212
                     /locus_tag="ACG808_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFS5046737.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            23209..24330
                     /locus_tag="ACG808_001095"
     CDS             23209..24330
                     /locus_tag="ACG808_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFS5046738.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            24345..25577
                     /gene="pepT"
                     /locus_tag="ACG808_001096"
     CDS             24345..25577
                     /gene="pepT"
                     /locus_tag="ACG808_001096"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFS5046739.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            25709..26617
                     /locus_tag="ACG808_001097"
     CDS             25709..26617
                     /locus_tag="ACG808_001097"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS5046740.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            26652..26924
                     /locus_tag="ACG808_001098"
     CDS             26652..26924
                     /locus_tag="ACG808_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFS5046741.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            26935..>27961
                     /locus_tag="ACG808_001099"
     CDS             26935..>27961
                     /locus_tag="ACG808_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFS5046742.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKE"
BASE COUNT         9397 a         4834 c         6099 g         7631 t
ORIGIN      
        1 aatgatgaaa aaagggggag aaaaatgaat tttttagttg ttttaaaaga tactctgatt
       61 gagcgatcta catttctata tgttcaattg caggaaaata aaacattgct gcatttttca
      121 ccagagttcc atctagaggg gctaacatta ggagaaatct atcaagcaga agggctgtcg
      181 gctgttttgc acttttttaa ggtagaattg gaattaccag taaaagatta tatcgagctt
      241 tctcttgaat cttgggatcg agcagtggtt gaactgtttc cagaaggttt aatgatcgat
      301 acaaacgatg ggaagatcca tctgactgca gcagaactgc aaaaacaaat gcgttatcaa
      361 atagatgacg aaaattcttt ttctattttt agacgtcagc aaaagattct aaaaagcttt
      421 ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga cacagctgtt gaaaaaatat
      481 ccaggtctgc ttcacacgtc tattcaattt cctcaattga ttacgatgat ccatcgtttt
      541 atacgtatgc aacagctacc atctaagaag ctgtttcttc cattgacgga cacatttcga
      601 gtagtcaaca gagaagataa aaagaaagtg attgtgattg actttaccag aaatcggaat
      661 gtccttcacc aagttgcaat ggaaaaggcc aattaaggct ttttcttttt ggattttttt
      721 gtcataataa atattagatg atggaggaga taatatgatg caaacatggt tagaaaggct
      781 aatacgattt cagcagaact atcacaaact tatgcaggga agatatgcga aatttgatca
      841 gttgaatcgc ggtctattga tcgctgcgct tgtctttagt ttgctaagtc gttggttgcc
      901 ctatagaatc ggacaattgt tgttcatcgt ctttttcgga tggatgatat ttcgtttcct
      961 atcaaaaaaa atctacccca gactaaatga gaatcaacgt tatttgaaat atcttgatag
     1021 agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg atcgaaaaag
     1081 ttatactttt tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta aaggaaaagg
     1141 acgaatacgt gtgacatgca aaacatgtgg tataaaattc gaaacgaatg tttaagggag
     1201 aaaagaatga aattagtatt attacgtcat ggcgaaagcg aagcaaattt tgaaaattac
     1261 tggacaggat ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc agcagttgcc
     1321 ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg tttatacctc tgttttgaaa
     1381 cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg gtcagttatg gcttcctata
     1441 ataaaaacat ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa aaacaaagat
     1501 aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg atatacggaa
     1561 gttccgccgt taaccgcaga aaatagattt gaccgcagat atgatcactt ggatccgcat
     1621 ttgatcccat acggagaaag tctggaaatg acagtgaatc ggatcattcc cttatggcaa
     1681 gatcatctag caccaaaact aaaagatcgg caagatttac tagttgtggg tcatggtaat
     1741 agcatccgag cactgacaaa atatttagaa gatgttcctg aagatcaaat ggatacgatc
     1801 gatatcccaa atgcacaacc aatccagtat acattagcag atgatttgac aattctggac
     1861 aaaaaaatac tttaaagaac acctttatct aaaagtgaga gatagaaaaa agaagcaaag
     1921 gaatggaaca aaaggaacat gaaaaattat atatgggact ttgatggtac attgtttgat
     1981 acgtatcccg caatggtaga tggtgcatgg caagcactaa aagattttgg tatcagtatg
     2041 gacaaaaaag aaatctactt caaaatgaaa aagtattcta ctagttattt gatcaatgaa
     2101 tcaaacttga atgccagaga gtttaatgaa ttatttcacc gatatgaaaa agaaagtacg
     2161 gaagtttccc gtccatttcc agaaacgaaa caagtactgg aaatgctaaa agacaacggc
     2221 ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa catggggcct gttaaaagaa
     2281 catcgattgg cacatctgat tgaggaagta gtagggatcg atcaggactt tcctcgtaaa
     2341 cctgatccag catctttaaa ttatttgatc gatacttttc atttagaaag aacagacacg
     2401 atgatgatcg gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg agtcgctact
     2461 tgtctttacg acatcgatca ttttctgggt gagatcccgg cagattatgt ggtaggaaat
     2521 ctgaatgaaa tccttacctt gcagagcatc attaaaaagt agtatcccct ttcaaaggag
     2581 atatgttata attttcataa aggagtgata aaattatatg gaaatcagaa gacaaaaaga
     2641 agttgtagaa gcatttcatt atgatatgag attgccgaac caagaagtag aaacagattt
     2701 gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag aacatagcat
     2761 cgtaggagca cgcttagagt tccgtttagt gtttgataca tatgttttat ctggaagtgt
     2821 gagtcagatc aatcatatcc tcaatcggaa aattgaaggt caaaatgatg tcacacaaga
     2881 agaagtggac gagctagtag caccgttatt cgatatcgta cgccgaatgt cttatgaggt
     2941 gacagagatc gccttagatc agcctggggt aaaattgaat tttcaatcag aaaaacaaga
     3001 atagacagta aaaacatacc tgcgatagat taaagctatc acaggtatgt ttttttatta
     3061 ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat aaaagtcact
     3121 gaaaaaaatt ttttcaggag gatggttgaa gaatcattct cgggaacctt tacttccttc
     3181 gatggacttg tcattaataa tatgaggagg agaaaaatga caacactcat cacagtttta
     3241 gtggttctag tttttgcggc attattatat gtgttctatc gtatgcaaaa gcgccataca
     3301 aaattttcaa cacgcgtctt tacagcatta ggagttggta tcgtctttgg cggtctgctt
     3361 caattgatct ttggtgtaga cgacaaagtc gttactcaat caatggaatg gatcgggatt
     3421 gtaggtcaag gatatgtatc attcttgcaa atgctagtta tgcctttagt gtttatttcg
     3481 atcattggtg cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat tagtgcgact
     3541 gtattgactt ctttacttgg aacaacagct attgcagcat tgatcggaat tggttcaaca
     3601 atgctgttcg gactccaagg agctaaattc acacaaggag ctgcagaaac agcacgaatc
     3661 tctgaactgg caacacgtca ggaaacagtt gaaaacttat cgattccaca gcagatttta
     3721 tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac aagtacgatt
     3781 ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa aagaaaagcg
     3841 ccaaaagaag cagcattctt ttcagattta gtcgacagtc tgtataaaat cattatgcgg
     3901 atcgtgacgc ttgttttgcg tttgacacct tatggagtat ttgctttgat gacaaacgtt
     3961 ttggcaacaa gtgattttgc agctatcgtc aatttaggta aatttgtcgt tgcttcttac
     4021 gtagcattgc ttgctgtttt gattgtccac tcactgattt tgattggtgc aaaagtcaat
     4081 ccaatcaact atttcaaaaa agcctttccg gtattgagct ttgcgtttac ttcacgttca
     4141 agtgctggag ctttgccatt gaatatcgag actcagacaa aagctttagg agtagatgat
     4201 gcaacagcta atttctcagc aagttttggt ttgtctattg gacaaaacgg ctgtgctggc
     4261 gtttatccag ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa tgtctttagt
     4321 ttggaattta ttttgatgct tgtggcaatt gtaacaatca gttcttttgg tgtagctgga
     4381 gtcggtgggg gcgcaacatt tgcttcactg atcgttttag gggcaatgaa cttgccagta
     4441 gcaatcgttg ggttagttat ctctgttgaa ccattaatcg atatggcacg tacagcagta
     4501 aacgtcagcg gtagcatggt agctggtatc gtgacaagtg cacgcatcaa agatttagat
     4561 cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga atgcatagtc aaaaagaggt
     4621 tgtgatagaa gtggtcagct ttaagaaata agaagaagtt tcctaaaaat agtttctcaa
     4681 atttttgtga aatttcggct tatttccgag aagctacttc tgaaacaccg tgaataagag
     4741 gttgtgaaaa agctgccctg catcaagtaa taagaacagc caaacaaaaa tagttcctca
     4801 tattttacgt ttggttgagc ttattaccgc agatgcaagc ttttgaacac cgtttaagaa
     4861 agaggtcgtg agcctgccgt ttcactctga gtcacaaaga gcaatttcaa aaaatagctt
     4921 ctcatgtttg ttgaatattg tgtcttgtga ccgaggagtt gtcaggagaa caccgtttac
     4981 cagaggttgt gacttgtttt tagtcacgac ctcttttttt atttattcca tataaataca
     5041 cattatttat tccgaaaagt gaaagcgcaa ataacaaaaa caaccaactt atttattatt
     5101 aaagaaaagc gtatttaagt aggacgattc tgttgagagc tttcgtagat tttcccattt
     5161 tcctatggta aactgacaaa gttgagaatg ttagaacaga tcgtttagga ggaaacagat
     5221 gtttacgaat gaaataaaaa tcatgcttta tgtggacgat gtagaaaaaa atgcaacgtt
     5281 ttggagagca gttggctttg cggaaaaaga tcgtcaagaa atggatggga cgcttataat
     5341 tgaagtagga gttgaggaca gtcaaacctc atttctgtta tatgatcgag catttgttga
     5401 gtcccattca cctggcacag ctactagtgc cccatctttg atgttttaca gtaaagatat
     5461 ctttggacta tataaaaaaa tgcagacgca atcagtcaca ttaggtgata tggtacagct
     5521 tggagaagaa tatgtcttta actttgctga tcccgatggc aactactttg cggttacagg
     5581 taaataatag aaataaagac tttgtttttc tgtgcgaaac aggaaagcga agtcttttta
     5641 attcaaataa taatattatg taaacaaaaa actttccatg cgtaattata cacaagcaaa
     5701 taaatcgtca aatgcgaacg ccttctttat actgtttagg aacgaaaaga aggaaggaag
     5761 aaatacatgg attatcaagt actgctttat tacaattata caaaaatcga agaaccagag
     5821 gaatttgcca aaaatcattt agccttttgc aaatctttgg atcttaaagg tcgtattcta
     5881 gtagctgaag aaggaataaa tggtactgtg tctggaaccg ttgaaaacac gactgcctat
     5941 atgaaagcaa tgaaagctga tcctcgattt gctgatacga tgttcaagat cgatccaagc
     6001 gaaggacatg cctttaagaa gatgtttgta agataccgtc cagagcttgt aagtttaaag
     6061 ctagaagagg acattgatcc aaaccaagtg acaggtgcct atctttcacc gcaagaattt
     6121 aaagaagcta ttttagatga acagacagtg gtgattgatg cacggaatga ttatgaatat
     6181 gacttgggac attttcgagg ggctgtacgc ccagatatac gcagttttcg agagcttcca
     6241 gactggatcc gcgagcacaa agaagaattt atggaaaaac gggtagtgac ctactgtact
     6301 ggtggcattc gttgcgagaa gttttctggt tggcttgtcc gtgaaggatt caaagatgtc
     6361 ggacagctgc atggcggtat tgctacttat ggtaaggatc ctgaagtcca aggagaatta
     6421 tgggacggca agatgtatgt gtttgatgaa cggatcgctg tcgatatcaa ccaagtagat
     6481 aaacaaatcg tcggtcgcga ctggtttgac ggaacaccat gtgaacgata tatcaattgt
     6541 agcaatcctg agtgcaatcg tcagattctt gcttccattg aaaatgaaga aaaacattta
     6601 ggagcatgta gcatagagtg tgcgaaacat ccgcgcaatc gctatgttca agaccatgga
     6661 atctctccag aagaagcaga actgcggttt acagctttaa ttaaatagaa aacaatagtt
     6721 ttgattgtcg accatttcca atatgcgcta tactagcgtt gaggatggtg atccacatga
     6781 aatcattgat ttatgctatt ggggatgtcc atggcaaaca tgagcttttt gaaaaactta
     6841 ttcactatta tgatccaaag atccatcagc ttgttttatt aggggatctc aatgatcgag
     6901 gtcctcgtac aaaagaatgt tttttattag gaaagcaatt agtcgaagaa acaggagcca
     6961 tttacctccg gggaaaccat gaagaatatt ttttgcaatt tctgcaatca ccggaggact
     7021 ggtatgcgcc ttatatgaga aatggtggaa aagaaacgtt tgaaagcctg attcacccag
     7081 gggcatcgga ggaatactcc ccaactgaaa tctcgatgat gatccaatcg agatattcag
     7141 aactgattaa atttttagtc cagcgcccct tgtattatga atgggaaaat tatctttttg
     7201 tccatgccgg tgtggatttg acaaagaaag actggcgtaa gacaagtcta agagacttta
     7261 tttggataag ggaaccattt catgaaggga aaaacaatac aggtaagaca attgtatttg
     7321 gacatacgat tacaccaatg ctccatggag atatgcagac aacagacctc tggattcagg
     7381 atcataaaat cggaatggac ggtggggctg tattcggagg gtcattgcat ggggttgttt
     7441 ttgacaagga aggtattctt caagatattg aatatcagaa caacgaagga ccttggcatg
     7501 gaatctgact aatggaaaga atgaaggagc tgtgactttg ttgcggctct ttttattttt
     7561 atagagaata ttgtttcttt cagtagttta acccaggata aaaagtatga tagaataaag
     7621 aagcaatttt tagcagaaaa gaggcatttt aatgacggaa aatttgaacc agacgatcat
     7681 tcagcttgta caaaaagaac tttcagacta tcgtcctaag cagttgacta ctgtacttaa
     7741 tttgttaaat gaaggaaaca ctgttccttt tatcgcgcgt taccgaaaag aaatgactgg
     7801 aagtcttgat gaagtccaga ttcgtgaaat cgaagagcgg tatgcctatc ttgaaaactt
     7861 agaaaaacgt aaaactgaag tgattcggct aatcgatgaa caagggaaac tgactcccga
     7921 gttagaagca gaaatcaccc aagccgtgaa aatgcaacaa gtggaagatt tgtatcgtcc
     7981 atacaaacaa aaacgacgga caaaagcaac gattgctaaa gaaaaaggac ttgaaccact
     8041 cgcgaagtgg ctgatgcagc ttacagatgg agaggtgcaa tctgaagcag aaaaatatat
     8101 tgacaaagaa aaagaagtct cttctgccga agaagcgttg cacggtgctc atgagattat
     8161 tgctgaacaa gtcagcgata atgcaaaatt tcggacttgg attcggtctt atacgtataa
     8221 caaaggtatg tatgtcagca atgtcaagga tgagcaagca gatgaaaaag gcgtatatga
     8281 aatgtattat gactttgcag aacccgtcca taaaatggtt tctcatcgaa tattagctac
     8341 taatcgtgga gaaaaagaag atattctgaa agtattcttt caagtagatg aagcagcaat
     8401 tttagcttac ttggatcgcc aaatagtcaa aaatcctgcc tctccttctt cttcattcgt
     8461 cagagaagcc tatcaggata gttataaacg ctttattcag ccagcaatcg agcgagaact
     8521 gcgcaatgaa ttgactgaaa aggcagatga gcaagcaatc gctattttcg gcgaaaacct
     8581 ccgtaatctt ttgttgcaac cgccactaaa aggtaaagtt gtcctgggat ttgatccggc
     8641 ttatcgtact ggatgtaaac tagcagtcgt agatgcgaca gggaaagttt tagcaatcga
     8701 agttatttat cctcataaac ctgcagcgca agcaaaaaga gaagctgctg gaccagcttt
     8761 tatccaatta attaataaat atcaagtaga tatggtagct atcggaaatg gtacagccag
     8821 tcgggaatct gaactttttg tggctgagca gttgaaatcc gccgaccata aaacctatta
     8881 cgctatcgtc aacgaagcag gcgcttctgt ttactccgct agcgaaattg cacgaaaaga
     8941 atttccgcat ctacaagttg aagaaagaag tgctgtttct attgcccgtc gcctgcaaga
     9001 tcctttagct gaattagtca aaatcgatcc taaagctgtc ggagtaggcc agtaccaaca
     9061 tgacgtttct caaaaacgtt tggctgaaca attggatttt gttgtggaaa cagcggttaa
     9121 ccaagtaggt gtggatgtga atacagcaag cccgcagctc ttgcagcata tatcaggatt
     9181 gaataaaaca actgcccaaa acattgtcat ttatcgtgaa gaaaacggag aattcaccgc
     9241 acgaactcaa ttgaaaaaag tgccacgctt aggaccaaaa gcatatgaac aagcaatcgg
     9301 attcttgcga gtccctggtg ggaaaaacat tttggacaac acaggtatcc atcccgaaag
     9361 ttattctatt gcaaaagaaa tattaacttc agtccaactt tcagaaaaag agcttggtac
     9421 agaagaagca gtagaaaaat tgacacgatt atccgtagag aaactggctg aaagcctatc
     9481 agtcggagaa gaaacgttag ccgatatttt agctgggctg acacaacctg gacgagatat
     9541 gcgtgatgag atgccagcgc ccttattacg tacagatgta ttgagtatgg aagacttgaa
     9601 accagggatg gaattgactg gaacggtccg taatgtcatt gacttcggcg catttgttga
     9661 tatcggtgtg aagcaagacg gacttgtcca tatatcgaaa ctcagcaaaa aattcgtcaa
     9721 gcatcctaca gatgtcgtgt cagtaggaga tatcgtaaca gtttggatag aacaagtcga
     9781 tacgaaaaaa ggcagaatca gtttgacgat gctttcccct tatgaagagt aacaatgagg
     9841 agtaacaaaa attgacacaa gaagaactgc agcagttagt agaagagata tcattaaaaa
     9901 gcttccagct tcctttcaag caccaagcaa tgttcaatcc acgcttacgt acaactggcg
     9961 ggcgctatca tttaaacgat catcatttag attttaatgc gaagctattt gaagagtcag
    10021 acgaagagac gattgcagga atcatcaaac atgaactttg ccattatcat cttcatcaag
    10081 ctggaagagg gtatcagcat aaagatatgg atttcaagta tcttttgaaa aaaacaggag
    10141 ggttgcgcta tgcaccagcc ttgacgaaga aacagataaa gatggagtat tatcgatgcc
    10201 aagattgttc tgccacgatc tgccgcaaga gaagaatcga tgtacgcaaa tatcgttgtg
    10261 gaagatgtca tggagaaatc aaatggattg agacaaaaag agaagacttt aatgaagcac
    10321 aaaaaatcag atgaaaggat agaaggcctt tggaaaaaag tgagcagagg aatctaaaag
    10381 actggaatca gctattaaat tttttagccg cacctgaaga gggaaatttt gaggaactga
    10441 cggcacctac acttatttta gctggaaact gtcttcctat cttagctgat aaagcagcac
    10501 aaatgtattt aaatggccaa gtagatcaat tgtttttggt cggaggggtg gggcatgcga
    10561 ctagaatcct ttatgagaat tttgaaaagc aaggatttca ttttgaagat ggcatgagtg
    10621 aaagtgaaat ctgtcgtcag tatctaaaag aagtctatga tttgcccgat aaggcttttc
    10681 tgatagaatc gaagtctaca aattctggag agaacgccac tttttcactg gagattcttc
    10741 attccttagg agctgtacca gaaaaaatct tattgatgaa tgatccgaca ctgcaacgca
    10801 ggactcgagc aacttttgag aaagtatggc aaaatgaaca aactattttc gcgaatgctg
    10861 tgccatttgt gccggaaatc cttcactttt ctgaagagat catctttaca gcaaaagaac
    10921 tgaatcatca atggcctaag gaatatttcc atgcgctagt attaggagaa atggaacgtc
    10981 tccatgacga tgagaacggc tatggtccga aaggaaaaga ttttattcct cacattgata
    11041 tatcagagga agtttggtct tcttacacca gaattaaaga gtcaataaaa acggattttt
    11101 ctcgtacttg accattttag caagcgaatc ctcctttcca aataaatttg gttgaaattt
    11161 tcaatcgttt ataagataat gaggagcgtg ttgaatgtaa tagctaaaag aatggacgct
    11221 agataagaga agaggattca aatggaaaat caaaataaat tgtatcattt tgttggtatc
    11281 aaaggttcag gaatgagctc cttggctctt gttttacatg aacaaggtct aaatgtccaa
    11341 ggatcagaca ttgaaaaata tttctttaca caaagagatt tagaaaaagc aaatatcacg
    11401 attctgccat ttaacgcaga taatgtaaaa ccagggatga cgatcattgc aggaaatgca
    11461 tttcctgatt cacatgaaga aatccagcga gcaaaagaat taggtttgga agtgattcgc
    11521 tatcacgact ttattggtca ttttatccaa aattatacga gtatcgctgt aacaggctct
    11581 cacggaaaaa caagtacgac tggactgctc tctcatgtat tgagtggtgt ccgtccaaca
    11641 agttatctaa ttggagatgg aacaggacat ggtgatccgc aagcggaatt cttttcattt
    11701 gaggcctgtg aatatcgccg tcatttcctt gcctattcac cagactatgc aatcatgacc
    11761 aatatcgatt ttgatcatcc ggattattat acaagtatcg acgatgtatt tacagctttc
    11821 caaacaatgg ctggacaagt gaaaaaagct atttttgcgt atggtgacga tgcttattta
    11881 cgcaaattaa aagcaaatgt gccgatttat tattatggtg tcacagaaaa cgatgatatc
    11941 caagcacgaa acatcgaacg cacaacttct ggttctgctt ttgatgtcta tcatggggat
    12001 gaatttgttg ggcacttcac tgtaccagca ttcggtaaac acaatatctt aaatgcttta
    12061 ggtgttattg cagtagctta tttcgaaaaa ctggatctga aagaagttgc agaagaaatg
    12121 ctgactttcc caggagtaaa acgtcgattc agtgaaaaga tcgtagcaga catgaccgta
    12181 gtcgatgatt atgcgcatca ccctgcagag atcaaagcaa cgattgatgg cgcacgccaa
    12241 aaatatccag ataaagaaat aattgcagtt ttccaacccc atacgtttac gcgtacgatt
    12301 gcattgatgg atgaatttgc agaagcactt gatttagctg ataaagttta cttatgcgat
    12361 atttttggtt cggctcggga agaacaagga aacgttaaga ttgaagacct tggagcgaaa
    12421 atcaaaaaag gtggagaagt catcaaagaa aataatgtat ctccattgtt agattatcat
    12481 gatgcagtag taatcttcat gggtgctgga gatgttcaaa aatttgaaca agcgtatgaa
    12541 aaacttttaa gcagcacgac gaaaaatgta ctgtaataca attactgcag tgtccttata
    12601 aaaatagaaa gagggggagt ggcaaaaatc ttgccaagcc cccttttttg caacttatga
    12661 taaacggtgt tcaaaagctt gcatctgcgg taataagccc aatcaaatca aaaacatgag
    12721 aagctgtttt ttcttggctg ttcttattat ttgatgcagg acagcttttt cacgacctct
    12781 gtaaactata ggaatctgtt aaatcaaggg agcagaactt gccttttacg ctacttttgg
    12841 tacaataatg tatgattaaa aataaactgg agggtttcta tcttgaacat aggtaagccg
    12901 agaaaactag gaaaaacaat aaatgaaatc gaggaaggcg actcgttatc cttaacagaa
    12961 tctattgaag ataaccagct tttgctgtat ttgggattga cgaatgatgc aaatcctttg
    13021 tatatccaac atgactttgc acaaaaaaca gaatatcaac agccaatcgt tccatcagtg
    13081 atgctgatgg ggatcatcac aagtgcaatt tcaaagcatc ttcctggacc aggatcacat
    13141 gttgtcaatt tttctgtcaa ttttattgaa ccggttttcc attatgaaac actgactttt
    13201 cagtttgagg tcataaaagt agataagatg aaggatgtgg tgacgatttc tgtagaagcg
    13261 gagaatacga acgaagatcg tgtgttggat gctgtcgtta tggtccaacc gccacaagtt
    13321 cctgaattgg aaatagcaat cgaaagtgaa ggtgaattga atgaataatc caagtacatc
    13381 aatgtcaggt atcaatcgtt tttatgcaaa agtttatgga tttttaggta tgggacttgc
    13441 ccttagtgca gtcacttctt acttggctct ttgggtattt cctttgcaat tagcaacttt
    13501 tatcaataat tttccactag gctttgtggg tctgtggatc gttgaactga ttttagtaat
    13561 ggttctaggc gtaaaagcac aaaagaatcc gagcttagca atcggtggat ttattgccta
    13621 ctctcttttg aatggcataa tattagcggt tactcttgca tactacgata ttggaacagt
    13681 aacgcaagct ttcttgacag ctactttgat gttcatgggg atgtctgttg tcggagtaac
    13741 aacaaaaaga gatcttagcg gggttggaag agcaggacag gcagctttaa tcggagtgat
    13801 cattgcacta gtcttgaaca tcttcatttt gagaagtagc gtagttgagc tgttcatctc
    13861 cctactgtta gtcgtcatct ttgcagggat tacagcttat gataaccaaa gcatccgtaa
    13921 tatctatgtc cagacaaacg gaacagcaca tagtggaatt gctgtattca tggcattgaa
    13981 gctgtatttg gactttatca acttgttctt ggcgtttctg agaatctttg gacggaacaa
    14041 ctaaaaaaga aaaaaattca tcagttgggg ctataccgaa ttatttggta tggtcccgct
    14101 ttttcttttc gcattccgca ggattctttg ttagaatgaa taagaacttt acagtaagga
    14161 gttaagaaga tgacaaaaaa taaattatta ctcgttgatg ggaacagtgt ggcttttcgc
    14221 gcattctttg cgcttcacaa ttctcttgaa cgtttcaaaa atagaaacgg tcttcataca
    14281 aatgcaatct atgcattcaa tacgatgttt gaaaatgtta tgcaaaaaga gcaaccgact
    14341 catgttctgg ttgcgttcga tgcaggaaaa acgacttttc ggacagaaat gtatgcggag
    14401 tacaaaggtg gaagatccaa aacacctggc gaattcaaag aacaaatgcc atatatacgt
    14461 gatttgttga caggactagg ggtacagtat tacgaattgc ccaattatga agcagatgat
    14521 attatcggta ctttggcaga aaaagtagac aaagatcaat ttgatgtcgt cgtgctttca
    14581 ggtgatcggg atttgactca attagctagt aaagaagtaa aagtggatat cactgtaaaa
    14641 ggtgtcagcg atatcgaaag ctatacacca gaacatgtag cggaaaaata tgatgggttg
    14701 acgccaaaac agattattga catgaaggga ctagccggag atgcctctga caatatcccc
    14761 ggtgtgacta aaatcggtga aaaaacagct atcaagctat tgaaacaata tggctcagtc
    14821 gaaggaattt atgaacatat cgatgaaatg aagcaaagca aaatgaaaga aaatctgata
    14881 aatgacaaag agcaggcttt cttatcgaaa aaattggcga ccatcaatac ttcagcgcct
    14941 gtggatgtct ctatcgactc attgaagtat gaagggaaaa atctcgataa actcgttccg
    15001 ttttataaag aaatggattt caatcagttc ctttctaaat tgaatatcgt agaagaagaa
    15061 gtaaaaatgg atgatatttt gtttgaagtc gttcatgaat tcaaagagga aatgtttact
    15121 acggatatgg cgttgtacgt ggaaatgatg ggcgacaatt atcatacaga ggaaatcgtc
    15181 ggagtagcgt gggggacaga aaagaaaatt tatgtcacga acgatctatc tgtattcgaa
    15241 aaccgagcgt tccattcttg gatcactgat ccgactcggc tgaaaaaagt atatgatgcg
    15301 aaacgaactt atgttgcttt aaaccgctat actggaaaaa cgaaaggaat cgattttgat
    15361 gtcttgctag gtgcatacct tctcgatacg aatgaaaaaa gcacagatat cgaaggaata
    15421 gctgctcatt acggatacaa cgatatccaa tcagatgaag ctgtatacgg caaaggggcg
    15481 aaaaaaggtt taccagaaga agaggaactc ttttttgctc atttagcgcg aaaagtagct
    15541 gcaatcaatg cgttgacacc aaaattgagc caagaactgg cagaaaaaaa ccaagaagat
    15601 cttttccgga aaatggaact gccattgtct atcattttgg cagagatgga aataagcggg
    15661 atcaaagtag atgcgactcg cttacaagaa atgaagggcg agttctctgc tcgcttaaga
    15721 gagatcgaac aaaaaatcta cgaagaagcc ggcgaagaat tcaatttgaa ttcaccaaag
    15781 caattaggag tgatcttatt tgaaaaaatg ggattacccg ttatcaaaaa aacaaaaact
    15841 ggatattcca cagccgtaga tgtactggaa cagcttcgtg aacaagcacc aatcgtagag
    15901 gatatcctca cctatcgtca aattgcaaaa attcaatcta cctatgtcga gggactgctg
    15961 aaagtgatcc aatcagatgg aaaagtgcat acccgttatg tccagacatt gacccaaaca
    16021 ggacgattaa gctctgttga tcctaatttg cagaacatcc cgattcgatt agatgaaggg
    16081 agaaagatac gtcaggcatt tgttcctaga gaaaaagact ggctgattta ttcttctgac
    16141 tattcacaaa tcgagttacg cgtacttgcg catatctcaa atgatgaaca tttgaaagct
    16201 gccttcctag aaggtcaaga tatccatgcc agcacagcga tgcgtgtatt tggaatcgaa
    16261 aaggcagagg atgtcacacc aaatatgcgt aggcaggcaa aagcagtgaa ttttggtatc
    16321 gtgtatggca tctctgatta cggactttca caaaatctag gaatcagccg aaaagcagca
    16381 cagcaataca tcgatacgta ctttgaaaaa tacccaggtg tcaaagaata tatggaaaga
    16441 atcgtacgcg aagcaaaaga ccaaggctac gtcgaaacgc tgtatcaccg ccgtcgatat
    16501 ctgccagaca tcaattcaag aaattacaat atccgttctt ttgcggaacg aacagctatc
    16561 aatacaccaa tccaaggaag tgctgctgat attttgaaaa tcgcgatgat cgaattggat
    16621 aaacgattaa aagaaacagg tcttcaagct acgatgctgt tacaagttca tgatgaactt
    16681 gtcttcgagg taccggaaaa agaacttgaa tcattagata aattggtgaa ggaagttatg
    16741 gaacaagctg tttctcttca tgtaccactg atcactgaca gcagttgggg aaaaacgtgg
    16801 tacgaagcaa aataaacatt gtttaagaca gagcaatata atgaaggtga aaagaaaaaa
    16861 tgccagaatt accagaagta gaaaccgttc gtaaaggatt ggaacggtta gtagtaggaa
    16921 aaacgattca aaaagttcag gtcttgtggc ccaagatcat cgagcaacca gagacaccca
    16981 tctttgaggc ttctcttgtt ggcgaaacca tccaatccat tggacgtaga gggaagtttt
    17041 taatttttca tctcgatcat tgtgaactga tctcacactt gcgaatggaa ggaaaatatc
    17101 aatttacgaa agaaaataca ccaatcgata aacatacgca tgtacttttc ttttttgagg
    17161 atggaagcca acttcgctac aacgatgtac gaaaatttgg tcgtatgaca atcgtcgaaa
    17221 aaggagcaag tgcaacttac cgaggaatca tgaaattggg acctgagcct ttgccagata
    17281 gcttcttatt agcagatttc gcaaatggac tgaaaaagtc ccacaaagca atcaagccat
    17341 tgctgctaga ccaacgtcta gtgactggtc taggtaatat ttatgtagat gaagcattat
    17401 gggaagcgaa gatccatcca gaacaaccgg cgaacacact gcgccccaaa gaggtagaac
    17461 aattacgtct atcgatcatt gatgttttgg atcgtgcaat agaagccgga ggtaccacga
    17521 ttcgcagtta tttgaatgca ttaggagaat caggcggatt tcaagtagca ctgcatgtct
    17581 atcagcaaac tggcaaacca tgtatacgtt gtggaacgcc gatcgtgaaa acaaaagtgg
    17641 cacaaagggg gacgcactat tgtccgaatt gtcaaaggct aaaaatcgca aagtaggatt
    17701 tgttttaggt ttgacgggtg gaatagctac cggtaaaagt acggcagcca aagtctttca
    17761 gtctcatgga tttcctttga tcgatggcga tgtgattgcc agagaaactg ttgagcctgg
    17821 aacgcttgct ctgaggaaaa ttgtatcagt gtttggccaa gaggtattgc agcctgatgg
    17881 gcaactagat cgagggaaac ttgggatgat tgtttttcca tcaaaggaaa aacggcaaaa
    17941 acttgatcag ctattagatc cgtttatccg aaaagcgata aaagatcaaa tcgcctctct
    18001 aagttccaat catcctttag taattgtaga tatcccattg ctctacgaag gtcattatga
    18061 ccactatatg gatgcagtag ctgtcgttta tactacgcca gaaactcagc ttcagcgttt
    18121 gatgaaacgt aatcaactga cgctagaaca agcgcagcag agaattagta gtcaacttcc
    18181 aatcgaagaa aaaaagcagc gtgcggatat tttgttcgac aacaatggaa caaaagaaat
    18241 acttgtggga caaatcgaaa attggctttt tgctaatcgc tttctttgag tatctgtgtc
    18301 aattttgcta tcctgagtat atagaaagtg agggatagcg atgggatcat taaaaaatca
    18361 aaaggataaa gtcgtaggta acgcaaaaga aaaaatcggc gagatgctca ataaagaaaa
    18421 tctaatcgat gaaggtcgta cgcaatcaga aacagcagaa gaaaaagaaa agcagtacca
    18481 agcaagaatc gatgaaatag aagaccagca ggcagaagaa gaagcgaagc ggcaagattt
    18541 gactcaagaa cgaaaaaatc cgacacctga tcgtagtaaa acaccgctcc cagaaagtga
    18601 ccacattccc gatgagcaaa aaatcgagga acagcgaatg aaacactata caggaatcga
    18661 agagaagtta taaaatagta aaaaatcgag atgtgacttg aagcagatga gaaggtgttc
    18721 atctgcttca agttttttgt gctagatgac taaatcagct tttgtgtaag ttatttctca
    18781 gtaatcaagg tgatcagtat tggaaaattg aaatttagta aggacagggg acacaattac
    18841 gcatgaaagt agaaaaaatt gttcttatat ccgtgttttt tgggtaggac agtcgcgttt
    18901 gtgatttgtt tcgtgttata atacaactag ttgtaaagaa agacacatcc tttatataaa
    18961 atgcgatgca agaggtgaga caaaatgaga tgtcccagat gtcaacataa taattcacga
    19021 gtaatcgaca gccgtcaagc agatgatgga cgtgcgatcc gtcgccgtcg cgaatgtgag
    19081 aattgttcgt tccgattcac tacatttgaa cgaattgaag ctgctccgtt attagtaatc
    19141 aagaaaaacg gggaacgcga agagttcaat cgcgataaaa ttttaagagg actcatccgt
    19201 tcagcagaaa aaagacctgt agctatggaa caaatggaac aaatcgtcaa ccatgtcgaa
    19261 aaccgcgtac gcagtctcgg agaaaacgaa gtatcgacga atctaattgg tgaatatgtg
    19321 atggaagatt tagttgattt agacgaaatc gcttatattc gttttgcaag tgtttatcgc
    19381 cagttcaaag atatgtctgt tttcttaaaa gagttgcaag atatcgtgga taaagcaaaa
    19441 gaaaataata aagagaataa taaagaaaac aattaagaaa caaaaaggag gcatttcctt
    19501 ggaaaatgct tggaaagagc ttcaaccaaa aaatatttac caaggaagaa aagcggctcc
    19561 gctgagagaa gacagcaaga acgccttgct gtatctttat cagccactaa tcggcggaga
    19621 agccttgagt atctatttca ccctgttatc agaaatctct caagaatctg gcaaaggtcc
    19681 tgaagggatg catgcagatc tgcttagtag tctaggctgc ggtatcccgc aattttatga
    19741 tgcaagaaaa aagctagaag gaatcggctt actggatgtt tattttaaag aagatagtga
    19801 tctgggacct cgttttattt atgagctgtt agaaccactc attcctaaag catttttcaa
    19861 agactctgtt ctggcttttc ttcttctgga aaaagttggt gaacgacgat tccagaagct
    19921 tacccaattc tttgaaccta aaaggcatac agcagaggga tatcaaaaaa tcacaaaaaa
    19981 atttttagat gtttatcgct tcaatgaagc agtgtacgct gctaatcaag aaaacatcga
    20041 atcgctgaat aaatcttttg aagaatctga atcagtagca ttacttgaag cagattcgac
    20101 attcgattgg cagtttttga atgattggct ggcaaaaaaa catattgcaa gtcttgatga
    20161 tccaatcatc aaacaagtcg acatgtatca tcagctttac ggtttcgatg aattgacact
    20221 tggcgaactg atcagtcaat cttatgactt tagtgaacaa aaagtatctg aaaaagaact
    20281 gcaaaaagcc attttggcaa tgcagagacg tccggtgaag caaacaaaag aaacagttgc
    20341 ggtttctgaa aacagtaatg aaccctcgtc aaacgaacat ttgacaaacg cagcgcttca
    20401 gctgataaaa gaagcacaga gcattccacc gatgaaatat ctggaagcaa tcaagtcaga
    20461 aaaaggcgga tatgtttcta aacaagaaaa ctggttactg caagatcttg tttctcaatc
    20521 aggtctttcc agtagcgtga tcaatgtttt gctgaattat gtactagtga tcaagaataa
    20581 tgctagctta aatgccagtt atgtaaatac agtggcaaat gaatgggcac aaaagaaaat
    20641 cgccacagca gaagaagcga tccagcacat tcggcaaata agtaatcaag caaaacaatc
    20701 aaaacaaaag aagcaaacaa attatcaaac tggcaagcga aatgttcgac gggaaaagct
    20761 tcctgattgg gtcaaccagc caaaagatga aaaacagatc agcagcgaga aaaaagcaga
    20821 gattgaccgc cgttttaaag aatacctggc aaaaaaggag ggtgatatct agtggaagac
    20881 gtaggcaaag aattgaagag gatcatcagc aaacgcaatt atcaagaaaa gtatgctgaa
    20941 atgatcgctg aagtcttaaa tgatgcggat gttcaagctt ttctaagcgc acaccaagaa
    21001 gaattgacac aagcggatat tgaaaaaagt tacgctaaat tgtatgaatt cgtgcaagaa
    21061 aagcgtaaat atcaaaaaaa cgatccaacg atgatcgctc ccggctatga gccgaagctg
    21121 acgttgaatt ttcattatgt ggatgtcact tatgtaccaa cggaagcatt gttagcacgg
    21181 caaagacaag aagaaatcag aagccgagta aaagcaatgg atatgccaaa agatattcaa
    21241 tcggcttctt ttgaaaactt tgaacgtaca gatggacgag gatatgcgag cttagaagcc
    21301 ctagattttg tagagaaata tactgctgat ccaaaagggt atcataaagg actgtatcta
    21361 gtaggaagtt ttggtattgg aaaaacatat ttattaggag caacagcgag agaattagcg
    21421 attgctggat tcaccacgac gctagtccat tttccgacat ttgccgttga gatgaaacag
    21481 gcaatcggaa aagatcaagt ggcagaaaag ttagatgcag tgaaacgttc acctattttg
    21541 atgatcgatg acattggtgc agatgcgatg agtagttgga tacgagacga agtattcggt
    21601 gttatccttc aatatcggat gcaggaacag ttaccaacct tcttctcatc gaattttaca
    21661 atggatgaat tggaacagca tttgtccgtc actcaacgtg gagatgaaga gccgctaaaa
    21721 gcgaaacgga taatggaacg catacgttat ttgacaaaag aaatcgaaat gactggtcgc
    21781 aaccgacgca atggttgaac agttgacaag tccaactgac atttgaccta gtagtaaaag
    21841 caggtcagcg aatcattata tggacagaaa tgaccggaga caatcaccgc aacaagtaat
    21901 gcagaaactt aaacggagga ttttttatga gggctatttt ttctagtata tctgtagttg
    21961 gtctattttt tcttttagca ggctgcggta gcgcagatag caacacaggt tctgttgatt
    22021 cgattgatgc gggtcaaaca gtaaaaacaa gcagtacgaa aacggagaga caaagcatat
    22081 ttgtcggaac gattgaaagt atcactcaag tagaagcaga agaagaaacg cttcagttga
    22141 aactcgtcga tgtcatggcc aaagaagatc cagcgaatat gggaacgtca ttcagcaatg
    22201 acggagtgat cttaaatgct actgctgaaa aaataagcgt ggacataaat gaattgaaaa
    22261 tcggtgacaa agtccaattt acattagttg agatgccgat catgacgatg tctatcccac
    22321 cgcaagttcc tggagattcg attattgatg tttcattgaa caaataacca ataacatttt
    22381 ttcaaaaata agaaaatgtt gttttataga acgaaaaggt atatttttta aggagatatg
    22441 ggaatttttt tattcctagt ctccttattt tatttggaaa atgctataat tatctagaag
    22501 gagtgaagct atgaaccatt tagatttatc tcagcgctta gccgcagtgg gtgactttgt
    22561 ccctgaatcc gctcgtttag cagacattgg atcagatcac gcttacttac ccgttgcatt
    22621 gatattaaga ggaaaaatcg aatatgcagt tgctggtgaa gtcgttcaag gtccttatga
    22681 atcagcacgt aagcaagtca aaaaagatgg attagaagac cgtatcaccg tacgccttgc
    22741 aaatggtcta gatgctatcc agccacaaga tggtattaca gctgtcacga ttgcgggtat
    22801 gggaggttca ctgatccgag atatcctaga agctggcaag ttgaatcaac gcttaagcgg
    22861 acaagaacgt ttgatccttc agccaaacat cggagagcgt acattacgat cttggctgca
    22921 agaaaatcag tataaaatcc aatcagaaaa aatacttgaa gaaaataaaa aaatatatga
    22981 aatcattgta gccgaaaaag aacaaaaacc agttacttac aatgaaatgg agctgatgtt
    23041 cgggccattt ctgcttgaag agaaaagtga gatcttcaaa tcaaaatgga caagagagct
    23101 tcaacaaaga aaagctatcc gtgaacagct caaacaagca gctaatcaag aggcacgtca
    23161 aaaacaaatc gagcaggaaa tccaaatgat tgaggaggtt ttaaagccat gacaatcagc
    23221 ggacgcactt tcatccaaaa gtttgaaagc tattgtcctc agtggctagc tgaagaagga
    23281 gacccagtcg gactgcatat cggaacattg gataagccaa tccaacgagt aatgatgaca
    23341 ttagacgttc gtccagaagt ggtcgaagaa gcaatcaaga aaaaaatcga tttgctgatt
    23401 gctaaacatc ctccaatctt tcgtcctgta aaacggctgg ttactgatca gccacaagaa
    23461 aaaatgtatg cagatttgtt gaaacatgat attgccgtct atgcagcaca cacgaacatg
    23521 gatattattt gggatggact gaatgactgg ttttgtgaac ttctcggtat tgaagtggag
    23581 agttatttag taaagactca tgagattcat tataaaaaac tggccgttta tgtacctgtg
    23641 gatcatgcac agaaaatgcg tgaagtatta gctgcagctg gtgctggtac acaaggtgat
    23701 tataccggta caagttttac ctcgattgga cacggtcgat ttactccgga agctggtgca
    23761 caaccagcga ttgggaaagt agggaagaca gaacaagttc aagaggcaaa agttgaggtt
    23821 attttgccgg aaactatcga aaaacaagtg attcaagcca tgcggtcagc tcatccttat
    23881 gaggaacctg cttatgatct tttcgctata gatgaaccag tcgaaatgtt tgggttagga
    23941 cgagtaggtg aattgccgca agaaatttct atagaggcat ttgtagaaca agtaaaagaa
    24001 gcgttccaac tagatggact gagaatagtc cagccaaaaa atgcaaaatc ctctgtgaag
    24061 cgcattgcga tttgcggtgg aagcggtgaa aaattttatc cgcaagcaat cgcccaaaga
    24121 gcagatgtct atatcacggg agatatctat tatcatacgg ctcatgatat gcaaagcgct
    24181 gggttgattg caattgatcc cggtcattat attgaatcgt tatgcaagca acgatttatc
    24241 gagaaatttg aatcttggaa acaagaagaa aattgggata ttaacttttt cgtttctgaa
    24301 acaaatacaa acccatttca atttaattaa aaggagagaa tactatgtac gaaaatcttt
    24361 tgccacgttt tttacgatat gtcaaaacag aaacacgctc cgatgcaaca agcaaaacga
    24421 ctccatcgac tcagacacaa gttgcttttg cgcaagaatt gaaaaaagaa ttagaagagt
    24481 taggcttagc tgatgttttc tataatgaag ataacggatt cgttatggga acattaccaa
    24541 gcaatgttga tcatgaagtg cgttccattg gatttatttc ccacatggat acagcagatt
    24601 ttaatgcagt aggagtcaat ccacaaatca ttgaaaacta tgatggcgag tctacgatcg
    24661 tactagatgc ggaagaacgc tatacattga atacaaaaga ttttccaaat ttgaaaaatt
    24721 atgaaggcca cacattgatc acaacagatg gcagtacatt actgggagca gatgacaaat
    24781 cagggattgc ggaaatcatg actgcaatgg agatcttgat caataatcct tctatccctc
    24841 acggtaccat caaagtggct tttggtcctg atgaagaaat cggtgtcgga gcggataaat
    24901 tcgatgttga gcaattcgat gtagattttg cttatacagt cgatggtggt cctttaggtg
    24961 agttgcagta tgaaacgttt agtgctgctc aagcaaatat tacgatccaa gggaaaaatg
    25021 tacatccagg gacggcaaaa gatacaatga tcaatgctct acagttagca atcgattttc
    25081 ataatcagct tccagccgat gaagttccag aaaaaacgga aggatacgaa ggtttcttcc
    25141 atttgatggc attggatggg aatcctgaag aagcttctat gagttatatc attcgcgatc
    25201 atgatcgtga aaaattcgaa gcacgcaaag cacaaatcac acatatccaa gaagagttga
    25261 atcagcgatt tgatcaagaa cgaatcaaag tagatatgta tgatcaatat tacaacatga
    25321 aagaaatcat tgaaaaagat atgagcatcg tggaattagc gaaacaagca atgatcgagc
    25381 ttggtatctc tccagtgatc gaaccagttc gtggaggaac agatggttca aaaatctctt
    25441 acatgggtat tccgacgcca aatctatttg ccggaggaga aaatatgcac ggacgattcg
    25501 aatttgtttc tttacaagta atggaaaaag caacagacgt gattgtaaaa attgcagaat
    25561 tgaacacaaa gagttaaaat cgaaaaagaa cagcttatga caaggcgttc gtcccaatga
    25621 tggaatgtca tctgtctaag gctaaagggc aggacaatgg aattggttgt cccgccctct
    25681 tttttgaaga gtaaatagga ggtaaagaat ggaaaagctg ctagtttttt ataacaaaag
    25741 ttcagggaaa gatgagggtg agaaattagc acgctggttt caagattatg taaaagaaaa
    25801 gcagccagaa ttatatgtag aactagctga aactggccca tcgattgatg atgcggaatt
    25861 agtcaagaaa gcagaagcca tagaagctga tactttagta attataggtg gagacggcac
    25921 gattcatcat atcgtccaag cgttccaaaa aacattatct caataccatg tcggattact
    25981 tccaggtgga actgtcaaca accttgcacg tgttcttgag attcccctca aaaaagaaga
    26041 agctgctgac gtgatcctgg aaggcaatgt acggaaaata gactatggtc aagtcaatga
    26101 tgaagtcatt atctctacac tgacaatcgg aatcttagca gatacagcag tttggatcag
    26161 tcaagaagaa aaacagcgtt acggctcgtg gatctttatc aagaagtttt ttaagttatt
    26221 gctgaagaag aaaaaatatc atttggattt agaaacagac gaggagcatt ggaaaggaaa
    26281 atcccaactg ttgacagtca cgatgtctaa ctccgtgggc ggttttacaa attttgacga
    26341 ctcagcgacc ctagatgatg gaaaatttca tgtaacgatc gtaccttcct tagatatttt
    26401 ccgatttatt ttttatttgc ctagaatcat gagaggcaaa ttctatacga tccccggcat
    26461 tacttatttc acagccaaac gcatgaaaat ccaagcgcag gaaaaatctg tgggcactcg
    26521 aacggatgga gacacgacag atgatcttcc agttgaatta tctgttgtat caaaaggatt
    26581 aacagtttat tcaccaaaga aagaagaaaa agcttagaaa tagtagttaa cagaaagttg
    26641 gaagaagagt aatggatgta ttgttacaca atcctttgtg gttttacctt ttcattgtga
    26701 atttttactt attttgtctg atggggtatg ataaacatca ggctagaact agcggctggc
    26761 gtgttcctga gaataatttg atatttgtca gcctgttagg cggtggtttg ggaggattga
    26821 tctctcgaaa agttttccat cataaaactc ggaaaaagaa gtttactata ggattcgctg
    26881 caggtgtact tgttgatttg attctcattt attttttcca ttaaggagca ggagatgtcg
    26941 tctaaaggaa cgaaaactaa aatcattctg aatctgcttt tgctgattgt gatttttgcc
    27001 attatttatt atttaatcaa tcaatcattt gcagatatct ttgccgaatt gctgtctacc
    27061 agtgttgttg ttttggcagc ggctgttata ttagggacag tttaccaaat cgctgaaggg
    27121 cgctcaatca aagaaattgc tgaacctttc gcgccgaatt ttacaacgaa agatgggttc
    27181 ttgacctcta gctatattgc gttttaccga atcgtttcgt ttggtacagg aacgctgatc
    27241 tcggagattt atttttataa caaaaaagga atgaagctat cccagggagc aggggtgaca
    27301 gcactccata tgattatgta caaactggca gtgatcactt atgcattagc tggcttggtc
    27361 atccaattct ctctttttta ttctaatggt ccatcaatga tctggtttat catcgctggt
    27421 atccttttga cgatcgtgat tgttgccttt ttattggcga tgtccgtcag cttgaatgtt
    27481 caaatatttt ttgtcagctt atcgaataag ttatttaaaa gtgagcgttt gcggaaatgg
    27541 gtggatacat gcaatcagca gatctactct ttaagggaaa cggtccaaac cattatccaa
    27601 gacaggactg cgctattgcg gatttatcta tggaatatgt taaaacttgt attttggtat
    27661 gtacttcctt atttgattct gattgaaaac catcctaaaa tagattttct actgaccatt
    27721 tcgtttatca gctttgcagt agtgctttca ggggttattc ctacgcctgc tggtattggt
    27781 tcctttgaat ttgtctattt gttattattc agaccagttg ttggaacagt tgacgcagtt
    27841 tcttcgcttt tactgtatcg tttcagttcc tttatcttgc catttctcta tggatttgtg
    27901 tatgtgattg ctgaacggcg aagagtgatc aaagaagaaa taatagaagt aaaaaaagag
    27961 a
//
LOCUS       DAZUMK010000026        27601 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000026 DAZUMK010000000
VERSION     DAZUMK010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.026"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..1687
                     /gene="rny"
                     /locus_tag="ACG808_001100"
     CDS             131..1687
                     /gene="rny"
                     /locus_tag="ACG808_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFS5046743.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(1766..2572)
                     /gene="proC"
                     /locus_tag="ACG808_001101"
     CDS             complement(1766..2572)
                     /gene="proC"
                     /locus_tag="ACG808_001101"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFS5046744.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            2713..5286
                     /gene="mprF"
                     /locus_tag="ACG808_001102"
     CDS             2713..5286
                     /gene="mprF"
                     /locus_tag="ACG808_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFS5046745.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            5440..6291
                     /gene="ispE"
                     /locus_tag="ACG808_001103"
     CDS             5440..6291
                     /gene="ispE"
                     /locus_tag="ACG808_001103"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFS5046746.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            6536..7474
                     /locus_tag="ACG808_001104"
     CDS             6536..7474
                     /locus_tag="ACG808_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFS5046747.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            7497..8177
                     /locus_tag="ACG808_001105"
     CDS             7497..8177
                     /locus_tag="ACG808_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFS5046748.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            8150..8974
                     /locus_tag="ACG808_001106"
     CDS             8150..8974
                     /locus_tag="ACG808_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFS5046749.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            9141..9959
                     /gene="purR"
                     /locus_tag="ACG808_001107"
     CDS             9141..9959
                     /gene="purR"
                     /locus_tag="ACG808_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFS5046750.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            10462..11835
                     /gene="glmU"
                     /locus_tag="ACG808_001108"
     CDS             10462..11835
                     /gene="glmU"
                     /locus_tag="ACG808_001108"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFS5046751.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEAFFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            11929..12900
                     /locus_tag="ACG808_001109"
     CDS             11929..12900
                     /locus_tag="ACG808_001109"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFS5046752.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      12906..13008
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13074..13643
                     /gene="thiT"
                     /locus_tag="ACG808_001110"
     CDS             13074..13643
                     /gene="thiT"
                     /locus_tag="ACG808_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFS5046753.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNSWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(13724..14497)
                     /locus_tag="ACG808_001111"
     CDS             complement(13724..14497)
                     /locus_tag="ACG808_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046754.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            14763..16463
                     /locus_tag="ACG808_001112"
     CDS             14763..16463
                     /locus_tag="ACG808_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFS5046755.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            16433..16651
                     /locus_tag="ACG808_001113"
     CDS             16433..16651
                     /locus_tag="ACG808_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFS5046756.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            16671..17561
                     /locus_tag="ACG808_001114"
     CDS             16671..17561
                     /locus_tag="ACG808_001114"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFS5046757.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            17579..17863
                     /locus_tag="ACG808_001115"
     CDS             17579..17863
                     /locus_tag="ACG808_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046758.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            18350..18964
                     /gene="gmk"
                     /locus_tag="ACG808_001116"
     CDS             18350..18964
                     /gene="gmk"
                     /locus_tag="ACG808_001116"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFS5046759.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            18969..19274
                     /gene="rpoZ"
                     /locus_tag="ACG808_001117"
     CDS             18969..19274
                     /gene="rpoZ"
                     /locus_tag="ACG808_001117"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFS5046760.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            19527..21935
                     /gene="priA"
                     /locus_tag="ACG808_001118"
     CDS             19527..21935
                     /gene="priA"
                     /locus_tag="ACG808_001118"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFS5046761.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            21950..22441
                     /gene="def"
                     /locus_tag="ACG808_001119"
     CDS             21950..22441
                     /gene="def"
                     /locus_tag="ACG808_001119"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFS5046762.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            22434..23372
                     /gene="fmt"
                     /locus_tag="ACG808_001120"
     CDS             22434..23372
                     /gene="fmt"
                     /locus_tag="ACG808_001120"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFS5046763.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            23372..24730
                     /gene="rsmB"
                     /locus_tag="ACG808_001121"
     CDS             23372..24730
                     /gene="rsmB"
                     /locus_tag="ACG808_001121"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFS5046764.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            24743..25483
                     /locus_tag="ACG808_001122"
     CDS             24743..25483
                     /locus_tag="ACG808_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFS5046765.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            25480..27549
                     /gene="pknB"
                     /locus_tag="ACG808_001123"
     CDS             25480..27549
                     /gene="pknB"
                     /locus_tag="ACG808_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFS5046766.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
BASE COUNT         9052 a         4875 c         6024 g         7650 t
ORIGIN      
        1 cattagaata aagttgtgca tgttttttgt acacatatat gcgaagatgc atattgacga
       61 gtttagaata atttatcata gaagctgtat agatgataaa aatgaaaaag atagtatgga
      121 ggtggatttt atggtattca acattctcct cgctatcatc ggtttaattg tcggtcttgg
      181 tttagggttt atggtcgcaa agtcacgtca cgagaaagaa atagctggtg cacaatcatc
      241 agctgctgga atcatcgata gtgcaaaaaa agaagcagaa actctaaaaa aagaagcatt
      301 gttagaagct aaggaagaaa atcagaaata ccgttctgaa gttgaaagtg agctgagaga
      361 aagtaagtta gaattaaaat ctcaagagaa tcgtttgatt caacgcgaac aaacgctgaa
      421 tcgtaaagat gactcgttag aaaaacgcga gaactcactt gaaagtaaag aagaagcact
      481 tagttcaaaa cagcaattaa ttgaagagcg agaaaaagac gttgaaaaaa tgattgaaga
      541 tcaacaaaaa gaactagaaa aaattgctgg tttatcaaaa gaggacgcac gagatgtcat
      601 catgaaatcg actgaagaag agctgaatca tgagttaact ctaatggtaa aagaatctga
      661 acagcgtgcc aaagaagaag cagatcgcaa agcgaaaaac ttattatcat tagcgattca
      721 acgctgcgca gcagatcaag tttcagaaac gactgtttca gtcgttaccc ttccgaatga
      781 tgaaatgaaa ggtcgtatca ttggacgaga aggtcgtaat attcgaacgc ttgagacgct
      841 gactggtatc gatttaatca tcgatgatac accggaagca gttgttcttt ccggattcga
      901 tccaatccga agagaaatcg cacggatgac cttggaaaaa ctgatacaag atggacgaat
      961 ccatccagcg cgtatcgaag aaatggtcga gaaatctcgt aaagaaatgg atgaacgtat
     1021 tcgcgaatac ggggaagagg cagcgtttga agtcggtgct catacacttc atccagatct
     1081 aatcaagatc atcggacgtc tgcgattcag aactagttat ggacaaaacg tcttgaagca
     1141 ttcggtcgag gttgcaaaat tagctggtat cttagcagca gagcttggcg aagatatcca
     1201 attagcaaaa cgtgccgggt tgctccatga tattgggaaa gcacttgacc atgagattga
     1261 aggatctcac gttgagatcg gtgcggaatt agctgccaag tacaaagaaa acccagtagt
     1321 aatcaacgcg atcgcttctc atcatggaga tgtggaagct acttctgtta tctctgtcct
     1381 tgtagctgca gcagatgcgc tctcagcagc aagacctggt gcgcgaagtg aatcattaga
     1441 aaactacatc cgtcgtttaa gaagtctgga aaatatttcg aacagcttcg ccggtgtaga
     1501 atcaagttac gcagttcaag ctggacgcga agttcgggta atggtcaagc cagaagaaat
     1561 ctcggattta gacgctgttc gtttagtacg tgatattcgt aaacgaatcg aagaagaact
     1621 ggattatcct ggacacatca aagtaatcgt tattcgggaa actcgtgcaa ctgattatgc
     1681 taaataataa aaaatacaaa agaagagccg cggcaaaagc cacagctctt ctttttttgt
     1741 aaggcacgaa aaaacattgc tatcattatt ttttattcaa catctcaaga tctttctcga
     1801 tcgtcgtatt gattcctttg atgattgctg ggatgaatcc tgcttcttct aaggcaacta
     1861 cacctgcaac agttgtaccg ccaggagaag aaacttggtc tacaagttcc catggatttt
     1921 tatcacttga aaggaatttt tgacaagaac ccataattgc ttttgcagca atctcagtgg
     1981 ctgtctgttt ggacaaacca ttcagtacac cagtccgtcc catcgcatcg atcagcatgt
     2041 aaacgaatgc gggtgagctg ccagcaaggc caatgaatgt actgaaatct gattctgcta
     2101 aaggatagac gctgccgatt gcttcaaaaa tcatcgtagc ttgctgaagc tgttcctccg
     2161 tggcagcttc atttttgcaa atcgcagaaa cgcctgctcc tacgctgaca ttgacattcg
     2221 gcatcacacg tacgatggag gcctgaggat cttcaagcgc tgtttctaaa tctgttaatg
     2281 attttcctga agcaatagaa atgaataaga ctttctggct atattctctt agttcttcca
     2341 atactttttc aatgattttt ggtttcacgg ctaaaagaac gatatctgat tgtgatgcaa
     2401 cttcctgatt acttttgcat gcatggacgc cagtttcagc agcaaatttt tgtgcatttt
     2461 caaaatgtcc actggaaacg aacacctctt ctttcgagac tgttcctttt tttaataaac
     2521 catcaataat agcttgtgcc atattccctg tgccgataaa tcctactttc atgttatcct
     2581 ctcctttagc attaggatat ctttcttttt ctatcatcgc aagcaaagaa caggtctaaa
     2641 gtggaaaaat taaaataaat aagataacat tggggtatga cacaaaagta tacaagttag
     2701 gaatgggaaa atgtgaaaaa taaacgatac ttgatgctgc aatggataaa ggaacatagt
     2761 ctgattttaa aactgatttt tttcggctcc gtgttagtct ttgtggcaaa tcaagtcgca
     2821 aatattgcta atggcatgag ttggcaagat atttggcaaa ggatgggaca acaaagccgc
     2881 atgacgatcc tgctcatgat tctggctgga ctgctgggtg tcactccgat gttgttgtat
     2941 gattgggtga cgatcagagt cttagaaaaa caaggaaaac caaaaatgga aagaaaagag
     3001 tttcttcttg ctgcgtgggt aacaaacacg atcaacaatc tcgccggttt tggtggtgtg
     3061 gtaggagcaa gtctgcgggc tagtttttat ggaaagcaga caaatagaaa aatggtttta
     3121 gccactgtat caaaagtcgc actatttatg ctgactggtt tgtctgtatg gtcgtttctt
     3181 acatttattg atgtcttttt tattcagtca gaaagtattt ttcgaagcta ttggatctgg
     3241 cttttcggcg gtagtttttt agcaccagtc attttctttt ttgcttatct aaagagaaag
     3301 acattgttct tcgagtttta tccaaaaggc ttgcttggtc tgctactggc atcctttgga
     3361 caatggacgg gagcccttgc ggtatttttg atcatcggca agttgatgag aatagatatt
     3421 tcgatgcttt cgatctatcc tatgttcatg attgctacat tgataggaat gctgacgatg
     3481 gtcccaggtg gaatggggac ttttgacgtt ttgatgatct taggaatgag tcaattgtct
     3541 gttcggcaag atgtagcagt agtttggcta ttatattatc ggttgtttta ttatgttcta
     3601 ccttttatga cgggacttct attgttcatc acacatacag gagtcaaaat caatcgattt
     3661 ttagataaca ttccgcgact gtttttccaa aaggttgcgc atatcgtgct cgtaagtgct
     3721 gtctattttg cggggatcat gatggtactt ctatcaacaa tcactaatct gtctgcaatc
     3781 agtcggctat ttcaagtatt attgccgttc tcattcaatt ttttagatca gacagtgaat
     3841 ctattggttg gctttttatt actcggcctt gctagagcac tttcagtaaa agtcaaaaag
     3901 gcatttctgc ctacgattgc tttactatct tttggtattt taaatacaat cacacgtacg
     3961 ttatcttggc agttgatatt ggtatatgtc ttgattctgt tagctgtgtg gttatccaga
     4021 aaagaatttt atcgaacaaa attcgtttat tcttggggag ctataatctt tgatgctagc
     4081 ttgttcggct ttttgtttat cgtttacgca attgcaggct atcacagcgg acgcttttta
     4141 gtcggtcgaa taacaggaaa tcattttatt ttgtttcctt cagatgatgt gtggttctca
     4201 gggcttattg gtttgggcat ctcattgcta gctttagtgg cgctttatca atatttagct
     4261 gaagatacta cgaagcttgg cagtgagtac gaaaaagacc gttttgaccg tttattggaa
     4321 acatacggag gcacagctac aagccatcag cttcgcttgt caggttacag ttatttttat
     4381 taccaagaag acgaccaaga tcaagtcgtt tttggttatc agatcaaagg aaacaaatgc
     4441 tttgttttag gagatccagt tggcaatcct aaaatgatgc gggcagcaac gaatcagttt
     4501 atgaaacaag cagattgttt aggctatcag cttgcgtttt ataagatatc agaaagctac
     4561 gtagtgatgc ttcatgaatt gggttttcac ttcacgaaag taggtgaagc agggattgtt
     4621 gatctagcaa cagaagactt gtctcggctg agtcagcaac ttgaatatca acgtttgatg
     4681 aaagaaggat accattttac ttggtacgat caactgccgg aagaattaca tcaagctgta
     4741 aatgaagttt cacaaaaatg gcttcaaggt gcacgagaaa aatacttctc tgtgggacga
     4801 tttgataaat catatgtatt atctagcggt gtcggtattg tccgtaaaga tcaaaaagtc
     4861 gttggcttca tcaccgctca gccgatgacg aagaagcagg cgggatatga tttgttgcgg
     4921 gtcttaccta aagaatctga ggaattagct gactatctgt tagccaatct gtttattgta
     4981 tacgagaaaa tgggctactc ggaagccaat ctaggcttag ctccattagc taatgttgga
     5041 gaaacagatt tttctttctt tcaagaaaaa gtcatgcata tcgtatataa ttatggtaat
     5101 tttttctatt ccttccagtc tgcttatgaa aagaaattaa aatatgtcga tcattgggaa
     5161 ggacgttatt ttgcttatag gaaaggaagt aatttcggat ttgctaccat gcagctactc
     5221 agtctgatag gaaaagaaaa aggaaatgtt ccgagtttgg cagaagaagt gctcacagaa
     5281 ttataagaat cactagaact tgtttggatt atgaagagaa aattctgtga aagctgttga
     5341 aacgatgaca aaatcaacca ctaggcagac ataagctata cttttatttt tccctatgat
     5401 agaatacttg cgacttagac ttttcgaatg ggtgagagga tggaattgat tgaaaaagcg
     5461 ccagccaaga taaatttagg actggatgta ttatatagac ggcaagatgg gtatcatgag
     5521 ttggaaatgg tgatgtcgag cgtagacttg gcagatcatc tattttttga agaaataaca
     5581 gaagacaaaa tcattgtaga aacaaataaa gcatttttac cggtagatga acggaacaat
     5641 gtttatcaag ctgctttatt gctaaaaaat aaatacaata tcaaaaccgg tgtcaagatc
     5701 tttgttaaga agaatattcc agtagcagct ggattaggcg gaggaagtac agactgtgca
     5761 gctgctctaa gagggatcaa ccaattatgg aatctgggct gttcgttaga agaactggca
     5821 gaaatcggtt tggaagtcgg tacagatgtt ccttactgtg tctacggaag tacggcattt
     5881 gtcggtggca aaggggaaga aatccaagta ttaccttcta tgccacagtg ctgggttgtg
     5941 ttggtcaaac caagaatgag tgtctctaca cgtacagtgt ttagagaagt agatacgcaa
     6001 gccatccaac atcaagacat ccacggactt gctgaagcca tccgagagca agattatcag
     6061 cagatgatca aaaaaatggg aaacagttta gaagatataa cgggaaagcg gcatccagtc
     6121 atccatcaaa tcaaagagcg tatgatcaag tacggagcag atgctgcatt gatgagtgga
     6181 agcggaccaa ccgtttttgc cttgtgtcaa cattattcaa gagcgcagag agtatataat
     6241 gggttaaaag gtttttgtga ggaagtttat ttagtacgaa ccctgcaata aaatataaga
     6301 agtaaaatag gtgatgaaaa actgaggaat cctaaaatgg tattcttcag ttttttattg
     6361 tagtgaaatg gctagatatg agcagcaact catccatttt acgatttaat caagaatata
     6421 catgcttaaa tcagaaattg atggatttct attgactgtg tttttttatt ttgatacatt
     6481 agttcacgtt aataaatcgt aaaattccga tttgttcatt gaaaggagca tataaatgaa
     6541 aaaatatctt ttgacagcta cggtaatgat aggtgcttta ctgtttgcag catgtggaaa
     6601 tacgaacaag gaagcagaca aaaaagagga tttgacgatc gttacgactt tttatccgat
     6661 gtatgatttt acaaaagaga tcgtagggga tgaaggaaac gtgaaacttt tgatccctgc
     6721 tggaacggag ccgcatgatt tcgaaccaag tgcaaaagaa agagcagaaa tttccgatgc
     6781 agatgttttc gtgtataata gttcagacat ggaatttttt gtggattcgt taaaggattc
     6841 agtggatagc aaacagacct tgatgatcga agcagctaaa ggaatcgacc gtttagaatc
     6901 gcaagaagca gacgaacatg aagaatcaga agaagggcat ggtcattcac atgaatacga
     6961 tccacatgtt tggctcgatc cggtattagc tataaaagaa gtcagaacga ttgctgaaga
     7021 attaggcgaa aaatatccag ataaaaagga aacttttacg aagaatgccg atgcttatat
     7081 caaaaagctt gaagcactgg atcaaaaata ctccgatgag ctaaaagatg caacaaaccg
     7141 cacatttgtc actcagcatg ctgcttttgc ttatttagcg aatcaatacg acttaaaaca
     7201 agtagcgatt tcgggcgttt caccagatca ggaacccaca ccaagtcgct tagctgagct
     7261 aaaagaattt gtgaagaaaa acaacatcaa agtgatttat tttgaagaaa atgcctcttc
     7321 gaaggtagct gagacattgt cgaatgaaac aggagtgaag ctagaagtgt tgaatccttt
     7381 agaaagcttg actaatgaac aaatcaaagc aggcgaaaat tatatttctg tcatggaaaa
     7441 aaatctgaaa gctttaaaag aaagtatcaa ttaagtttta gaatggaggt tgtaaggtgc
     7501 gttatatcga aatatctgat ttgacctttt actatgatga tgaacctgta ctagaagatg
     7561 tctcttatca tgtagatgcg ggtgaatttg tcattttaac tggagaaaat ggagcagcca
     7621 agtcaacctt ggtgaaagca tcgttaggat tactgaaacc ttcaaaagga atgataaaga
     7681 ttgctgagaa aaatcaagaa ggaaaaaaat taagtatcgg ttatattcct caacaagttg
     7741 catctttcaa tgcaggtttt cctagtacgg tgatcgaatt agtccgttca ggaagatatc
     7801 caagaggccg ctggttcaag cgtctctctg aaaaagatca tcaacatgta cagcgtgctt
     7861 tagaagctgt agggatgtgg gatatgcgaa atcggcgaat aggtgaattg tctggcggac
     7921 aaaagcagcg aatcagttta gcgcgtattt ttgctactga ccctgactta tttgttcttg
     7981 atgaaccaac aacaggtatg gatgaagaat ccagaaatga attttatcgt cttctccgac
     8041 atagtgcgca tgtccatggg aaatcagttc taatgatcac ccatgatcat gaagatatca
     8101 aacagtatgc ggacagacag atccgactgg tgagaaagga ggattctcaa tggcgttgct
     8161 ttcatatgaa ttcatgagaa gagcgtttct tgccgctatc ttcattgccg gaatcgcccc
     8221 gatgctaggt gtgtttttag tgatcagaag acaatcgctg atggctgata cgctttctca
     8281 tgtctcttta gcaggggtag cgttaggatt ctttttgaac cttaatccta cacttacgac
     8341 catgctagtc gttgtattag cagctgtgat attggagtat ctgcgaacga tctaccgaac
     8401 ctattcagaa atctccattg ccattttaat ggctggtggt ttggcgctag cgttagtatt
     8461 gatgaatttg agcggaggga attcagcaac aagtatccaa tcctatctgt ttggttcgat
     8521 cgtgaccatt acatgggagc aagtcatctt tttaggaatc ttattcgttg ccatcggtat
     8581 attgttcttt ttattcaaac ggccgatgta tgttctgact tttgatgaag ataccgccca
     8641 cgtggatggg ctacccgttc atttgatgtc gatgatgttc aatgtgatta ctggtgtggc
     8701 tattgctgtg atgatcccga ttgccggtgc acttttgata tccgcaatca tggttcttcc
     8761 cgcagcaatc ggtatgcgat taggcaaaag cttcaatgtc gtgattgcta tcagtgtttg
     8821 tgtaggattt ctagggatgc tgtcaggact aactagctcg ttttatctag atacacctcc
     8881 aggagcaaca atcacattag tctttattgc tttgttcctg attgtaaata tcatcaaacg
     8941 aatattggtt gccttcagaa gaaaaaataa ctaaattata aagaaaagaa gccttataaa
     9001 aacgaaaaat tttttatagg cttcttttta ttttaataaa taccgaacta ttttcttatt
     9061 ttttcataaa atgctcgaat ttttgaagaa aaacttatat aatgtaaaac gataaaattt
     9121 cttaaaagga gttggaatag gtgaaagtac gtcgcagtga acgtctagtt gacatgacac
     9181 agtatttatt gaatcacccg catgaattga tttccttaac aagctttgcc gaacgttatg
     9241 aatctgctaa atcttctatc agtgaagatt tgggaatcat caagaaaaca tttaaagaac
     9301 gagggtatgg aaccttggaa acaatcccgg gtgctgcagg tggtgtattg ttcatccctg
     9361 aaatttcata tgaagaggcg aaaaaatata ttgaatctct agcagaacgt ctatcagaac
     9421 aagaccgtct gcttccagga ggatatgttt atctctctga tttgttagga gaacctgaac
     9481 tgctgcgtca agttggaaga atcattgctt ctaaatacct tggtgaaaaa atcgatgctg
     9541 taatgactgt agccacaaaa ggcgtaccta ttgcccaagc tgtgtcctat tatctgaatg
     9601 cgccattcgt tattgtacgc cgggactcaa aaataacaga aggatcaaca gtaagtgtca
     9661 actatgtgtc tggatcttct gaaagaattg aaaagatgga actgtctaag agaagtttga
     9721 aacgtggttc acgtgtcctt gtagtggatg atttcatgaa aggcggcgga acagtcaacg
     9781 gaatgaagag cttgattgaa gaattcgaat cagaacttgt tggcatcact gtatttgccg
     9841 aatcaaaatt caacggtcat cgcgccatcg atgattatac gtcactgctt tatgtcaaag
     9901 atgtagatac tcaaacgaaa aatattacgg ttgtgccagg aaattatttt tcagaataag
     9961 aggttgtgaa aaagccgctt agtttcgagt agtaagaaaa attttgtgaa aatggctttc
    10021 cacatttttt cataaatttg gcttaatacc gagaaactgg cttttgaaca ccgtttatct
    10081 agaggttgtg aaatcagcgt cctgtcctga gtattagctc aataaacgga aaaattgctc
    10141 ctcatatttt gccgtttatt gagctaatac cgaaggtcag ctgattgaaa aacgtttaat
    10201 gaagaggttg taagcctacc gtttaactct aagtcacaag aaacgatttc aaaaaacagc
    10261 ttttcatgtt ttatgagtag tgtgacttgt gaccgaggag ttggcaggag agcaccggtt
    10321 accaaataga taggcccgtt atggtctatc tattttttta tccagaaaac gctgggataa
    10381 aagaaaatca cttgttttgt tgaaaaaatg ggtatactgg gataaactga aatcagcaaa
    10441 ctatatttag gagtgacgca gttggacgca cgttatgcaa ttattttggc ggcagggaaa
    10501 ggtacccgca tgaagtcaaa attatataaa gttttacatc ccgtttctgg taaacctatg
    10561 gtagaacata tcattaatcg ggtcagtgaa acgaaaccag atgaagtgat tacgatcgtt
    10621 ggacacggag cagaacaagt taaagcacaa ctaggagaac gcagtaaata tgcgttacaa
    10681 gcagaacaat tgggaacagg acatgctgtt ttgcaagctg cttctttctt ggaaggaaaa
    10741 aaaggaacaa cgttagttat cagtggcgat acaccattat tgactactga aacgctgaac
    10801 aatctttttg aatatcacca aggaaaaaat gcaagtgcaa cgatcttgac ggcacaagca
    10861 gaaaacccaa caggatatgg acgaatcatc cgtgaccata ttgggatcgt agaaaaaatc
    10921 gtggaacaaa aagatgcaac accagaagaa gctcttgtac aagaaatcaa tacaggaact
    10981 tactgttttg acaacgaagc ttttttcgat gcgttaagca aagttggaac aaacaatgct
    11041 caaggtgaat actatctaac agatatcatc gaaatcttaa aagaagaagg ccatacagtt
    11101 gctgcttatc aaacggatga ttttgaagaa tcaatgggag tcaatgatcg gatcgcgtta
    11161 gctaaagcaa atgagatcat gagaaagcga attaaccaga tgcatatggt caatggcgta
    11221 agctttgtcg attcagcaac gacatacatc gatgcaggcg tagaaatcgg tccagacaca
    11281 ttgatcgaag caggtgtaca aatccaagga aatacagtca ttggttctga ttgtgtcatc
    11341 gggtcacatt caaaaatcgt agacagtagg attgaggatc acgtcgtcat tgaaaactca
    11401 gtcattgaaa gcagccatgt caagaaacat gcagatgtgg gaccttatgc acatttacga
    11461 ccaaaagctg aaattggcga aaacgtccat atcggaaact ttgtggaagt gaaaaatgct
    11521 caaatcggca aaggaactaa agtcggtcac ttgacttatg ttggtgatgc tacgctggga
    11581 gaagaaatca atgtcggatg tggtgttgtc ttcgtcaact atgatggaaa gaacaaacat
    11641 catacgactg tcggcgacca cagctttatt ggttctagca cgaatatcat cggacctgtc
    11701 gaggtagcta aaaacagttc gattgctgcc gggtcaacaa ttacagacaa tattccagaa
    11761 tatgcgctgg caattgcgag agcaagacaa gtcaataaag aaggatatgc aaaaaaactc
    11821 ccttatctaa actaagactt ttgcaaaatc acttgattta tttaaaaaat atgactacta
    11881 tttattaggt agaattctag agttgtgaaa aagaaagtgg aggtccccat gtcaaaacat
    11941 tactttgacc caagattaaa aatttttgcg ttaaattcca atcgcccatt agcagagaaa
    12001 attgcagcag cagtcggcgt cgagttagga aaatcatctg ttactcaatt tagtgatgga
    12061 gaaattcaag taaacattga agaaagtatt cgtggatcgc atgtctatgt tattcaatca
    12121 acgagcagcc cagtcaatga taacttgatg gagttgctga tcatgattga cgcactgaaa
    12181 cgagcaagtg caaaaacaat caacgtagtc atgccttatt atggctatgc gcgtcaagac
    12241 cgtaaagcac gtgcacggga accaatcaca gcgaaattag tagcaaacat gattcaaaaa
    12301 gctggtgcaa caagaatgct gactttggac cttcatgcag tccaaatcca aggtttcttt
    12361 gatattccag tcgatcattt gatgggtgcg ccacttattg ccaattattt cttagaaaaa
    12421 ggaatcaaag gcgatgatgt tgtcgttgtt tcaccagacc atggtggtgt cacacgggca
    12481 cgtaaattag cggaattttt gaaatctccg attgcgatca tagacaaacg ccgtccaaaa
    12541 gcaaatgttg cagaagtaat gaatatcatt ggacaagtca aaggcaaaac ttgtgtactg
    12601 atcgatgata tgatcgatac agcaggtacg atcacattgg ctgccaatgc tttgaaagaa
    12661 gctggtgcaa ctagtgtgta tgcttcatgt acacatccag tattatccgg ccctgcttta
    12721 caaagaatag aagattcagc tatcgagcgt ctagttgtta cagactcgat ctatcttcca
    12781 gaagatcgta aaatcgacaa aatcgatgaa gtaagtgttg gcggactaat gggagatgca
    12841 atcaaacgta tccatgaaaa caaaccagtt agtccattgt ttgaaacaaa aaataaataa
    12901 taaataaact cacaaagggg agtccaattg ggctgagatt gaatctattt ctaaaccctt
    12961 cgtacctgta tcggttatgc gagcgtagga attgtgaata aacagctgtt tgactatttt
    13021 cgaaagctgc ttcctccttt gtgacttttt tattcaaagg aggaagtttt tttatgggaa
    13081 aaaatagagt ttgggtagaa ggaacgattg ttgcagcgtt agcgatggtg ttgtcgttga
    13141 ttccgatcca gatcggcagc agtttttcta tttcattagg gcagattcca ttgactcttt
    13201 ttgcgttaag aagagggaca aaggcaggga tgctggcagg tttcatttgg ggcatcctac
    13261 attttccgtt gggacaagtt tattatttaa gtgtcgttca agtgttgatt gagtacccga
    13321 ttgcctatac ttttgcaggc gcagcagggc tcatggcttc aaatgtccaa aaaggctatg
    13381 tagacgataa tacgaaacaa attagaaaaa gcatcgtgtt tggtgcattg attggcgcac
    13441 tttccagata tttttggcat tttattgcag gagtggtttt ctggggagcg tatgcactat
    13501 gggggatgaa ttcttggctc ttttctttcg taatgaatgg tgccagcggg ttgagtacag
    13561 ctattgtaac gatcatcgtt ttattaggga tccaacaaac gactccttcg atttttgtgc
    13621 cagataaacg acaggagctg taaataaaaa agaagcaaga aacttgagac agaatcatgt
    13681 cctagtttct tgtttccttt ttattttttg ataagaaata gatctacttc aatggcagat
    13741 aagaaagatc ggcatcttct accaattgcc agtgtcgatc ggtgacagag tagattaaag
    13801 tagcgatgat ttcctcttct cgataccggg tgccttttaa tttccaaata taatcttcaa
    13861 tcacatagat tgggatacga tatttaccat cgatcaaacc aacttgatca aaagaaaaaa
    13921 tgatcccttc ctgcatcaaa ttacgcattt ctttcaataa ttccacactg attttttcaa
    13981 caggtgtttc ttttttgaaa aaagattttt ttacattgat cttatctgac aagatcgaac
    14041 ttaatttact agcaaaaatc ctataaaatt catctaaata gcgataataa atccgattca
    14101 gctgatcatt aagatcgaag tatacaaaat tattctgtag tttataaaaa aacggcgaat
    14161 gaagatgtgt tttcatatga ccaaaatata ataattctga aatttctaaa ggtgtcaatt
    14221 ctttcagcat cgttaaatca gtaaagtcga tccacttcaa ttcatcgaat ccacgcctgt
    14281 tacgagaaga ggaaaagaaa cgctgaatat tttctgtacc acggattact ttcagccctg
    14341 tatgggattc atattctcca gcagaagcag atggatcaag caagagcaag ttttgaggaa
    14401 aatgcacgat actgtttgca aagtcagtag cagtcagccc ttttgttaaa acagcattac
    14461 ttgtggtatc aatatgtaca taaactgact cagtcatcat gttcctcctc ttatttcctc
    14521 ttaatctctt ttattttaca acatttgcat taaaaaagct tgtttctttt attttaaaat
    14581 tccattacaa aatcacgttt tcattgaaaa cactctcatt ttcaattaat ttttgtgatt
    14641 tttgtatttt ttatttaaag gatgttttat gaattgtttt cttatggaat ctacaaagtc
    14701 gaatacgaga ctcatgacct ttttatgacg tactggtata cttgaagaaa aaggagagcg
    14761 cgatggagat aaaaaaagtt tacttttcac agaaaatgtc gctgtattcg cctatccagc
    14821 aagcaaaaca accccttcca ttttttcaaa gtcatcaaga aagtagagac gctttttggc
    14881 gttctgcaaa aaagcaatgt gcttttaaag cagtatcgaa aaaatatacc gtctatttta
    14941 ctattgctaa aagtagagga gatgttattt ttaataatct gttgattgaa ggtgaaactt
    15001 ttgaatggaa aaaatttttt cggtatatgg aagcttgcgc tcgttttttt attaaagaaa
    15061 actgttgttt tttatttcaa tctcctctat cagaagaatg gttgcgtatt tttcataaaa
    15121 acggctatca aggaaccaca caattaaata aaaagctggt ctatcataca gcacttgttt
    15181 taggtggagg aggagcacac ggtgcttatc agattggtgt gtggcaggcg ctgaaggaac
    15241 atgatatcaa ctttgagatt atcacaggta cctcggtagg tgcgttgaac ggcgcattga
    15301 ttttacaagg agatatgaaa aaagctgtga atttatggaa aaagctgtcg acaagacaag
    15361 tgcttgcgtt accagaaatg gctgcagttg aagaccttcg tgaacgcttc tatcgagaaa
    15421 gacgccaaat gacgaaaaca gcactaattg aaggcggggt ttcttctgct ccattggaaa
    15481 agatggtcaa agacaatttg gatttgtcct tactcaaaca taatcccaaa atacgtttat
    15541 atacggtgtc tactaaactg ccagaactag cggaagtagt gactgatatc caacagacaa
    15601 aaacagaaga aataccagat tggatactcg cctctgcttc attttatccc gctatggctt
    15661 atcgaaagat cggaaaaaat aaatatgcag acggtgggta tcgcaacaaa atacctattg
    15721 atattgcgat taacaaagga gcaacagagg catttgtcgt agatgtgcaa gggccaggcc
    15781 cagcgaaaaa aattcgtatg ccagacattt ttatccattg gaaatgtcaa acattgtgga
    15841 cactaggcag ctttctgctt tttgatagcc aaaggaacca attaaattta caattaggct
    15901 atctggaaat gaaaaaacga ttaggctatt actatgggaa ctggtataca tttcattctg
    15961 ttaaacctgc aagaacatac tggcgaggat ttttgtctta cttaacaaaa gaaattcagt
    16021 tagaattatc ttttttcaaa agcataaaat tttggcaaaa actgcgtaat ttatataaga
    16081 acagggtcgt accagagaca tgcggattgg cgatgctaga acttttagca aaaaaacatt
    16141 ttttactgcc caatgaaatc tatcaagtag acatgatgat tcaaatgatc tgccaacaag
    16201 atataaaaag tatgccggat gatttattgg cacaaattgg tcaattaagt actgaagaat
    16261 ggcgaaggta tcgaaaatac cagcaaaaaa tacaaaatga gcgcacgaaa actgtttatt
    16321 ttgattatct gctacaagga aaacgcaagg atcgtttgca gcatgaattg cttaagcaac
    16381 cagttgacac attactgatc ctatatttat attatctcaa ggaggaacaa ccatggcaca
    16441 agaattttca tatgaaatcg tagaagaaat cgctatattg tcagaaaaca acaaggggtg
    16501 gcgtaaagaa ctgaacctag tcagctggaa tggtcgcccg ccaaaattcg atcttcgaga
    16561 ttgggcgcca gaccatgaaa aaatggggaa aggcctgaca ttgacgaacg aagagtttga
    16621 acaactgcaa aaagcaatag aaaatatgta atgaggaagg accctgtcga atgaaaagta
    16681 tgactggttt tggcaaagcc atccgcgaaa caaaagcgta tcagttagat attgaaataa
    16741 aaagtgtcaa ccagcgtttt ctagatgttc aaatacgaag tcctaaaatc ctgaactttg
    16801 ttgaaaacga ttttcgtcag cttattaaac agcagttaag ccgcggtcga atcgaagtat
    16861 ttgtgaatct agcttatatt ggcaaaaacg aaaaacagat cgtcatcaac tgggaattga
    16921 ttgattcact tatcactcaa ttgacagacg gaattcaaca acgatacggt agtcaaaatc
    16981 aagtgtcaat tagtcggatg gcggaacgct tcgcgctgga cgaaaacttc gtaacggtag
    17041 aagaaaaacc ggaagagagc atggaagaat tggcacagtt agctctagat acattgaaaa
    17101 gcgcacttga ttctattgag gcgagtcgag aaaaagaagg tcaagcttta gctttagtgt
    17161 tgaaaaagaa tactgaagaa ttcaaagaag tactaaaaaa actcgatagt tttgtagaaa
    17221 tctatgaaaa ggaatttcaa ataaggtatc aaaaaaagct ggaggaattt cttggagcga
    17281 aagtcgatga gcaacggttg ttgacggagc tagccatctt gttggaacgc ggagatattc
    17341 acgaggaact ggatcgtctg gcgatccatg tacaaaaact agatgaactt atcgaagcac
    17401 aatgtcctgt aggtcgggaa ttagatttcc tgatccaaga aatgaatcgg gaaatcaaca
    17461 cgatcggttc caaatcaaat gcaatcgaaa ttaaaaatca agtcgtccag tctaagacga
    17521 tcttagaaaa aatcagagaa caagtacaaa atattgaatg acgaaagagg tagagataat
    17581 gaaatattat gacgtaacgt tccacgaatt aagtggaaaa actgtggtta aacgggatat
    17641 cccttctgaa aaaaacggct ttgacgtatg gaaagatgcc tgtgcagact ataatgagaa
    17701 cgaactgttt attttgatca acgacggtgc ttacgtgacg atgaatcgga aatttattgt
    17761 tcgaattgac acagaagagg tagaagatcc gacagaaaaa gcacgttctc gaaaagatga
    17821 gatcatgggt gtagtgaata ctttgtccaa tatgggcttt tagcaaggat gctgaacaaa
    17881 gaaatacaat aaataatcag aattggattt cttcgcttag cttctttgct ctaggaatac
    17941 atttaaaggt tgtgacaaaa ttcgtgtcac aatctttttt tattttgaat aagtgatgtt
    18001 caaaaaccat tttctcggta ttaagccaaa tttatgcaaa aatgtgagaa actattttca
    18061 caaaattttt cttcatactc gaaactaatc ggcttcttca cgacctctta tacacggcgt
    18121 tcaaaagctt gcatctgcga taataacccc aaccaaacga aaaacatgag aagctgttta
    18181 tgtttggctg ttcttattac ttgatgcagg acagcttttt tacgacctct tggtacaggg
    18241 tagctttttt accaatttct agctataatt aaagataacc ttgaaagttg cagaaaaaaa
    18301 gtgcaaaata aacgataagt acaaaaagac tagttgaaag gacgtcgtca tgtcagagcg
    18361 tggattatta atcgtgttgt ctggtccatc cggtgtagga aaaggcacag tacgaaaagc
    18421 aatttttgag agtgaggata atgattttca gtattcgatc tcaatgacta cccgtaaaat
    18481 gcgtgaaggg gaggtcgaag gcgttgatta tttcttccga agcaaagaag agtttgaatc
    18541 aatgatcgaa gctggagaga tgctggaata cgcagaatat gtcgggaatt attatggaac
    18601 tcctttgtca tatgtcaaca agacattgga tgaaggaaaa gatgttttct tggagattga
    18661 agtccaagga gcaaaacaag tcaaagaaaa agtgcccgat ggtgtattca tctttttgac
    18721 tccgccagat ttagcggaat taaaatcacg gatcgtaggc cgaggaacag atgcagatga
    18781 agtaatcgaa gaacgaatga aagttgcaaa agaagaaatc gaaatgatgg ctctttacga
    18841 ctatgctgtt gtaaatgatg aagtgccgct agcagttgaa cgaatcaaaa acattattgc
    18901 cagtgaacat tttcgagtag atcgcgtaat cggtaaatat attaaaatgt taaaggagat
    18961 gtagtcttat gatgttaaaa ccttcaattg actcactatt agatcgtgta aattcaaaat
    19021 attctctagt gattttagca agtaaacgcg cgcatgaatt ggatgcaagt gcacagccta
    19081 ctctagattc ttttgattca gtaaaaagcg tcggtcaagc tttagaagaa atcgatgcag
    19141 gaaatgtagt caatgatcca catcctgaat taaaacgtga acgtttgaag atggaagaag
    19201 aggaacgtca agcacaaaaa gagcgtgaac aacacgaatt ggaatcacgt atccgtgaag
    19261 agcaaaaaat gtaataaaga aaaatagagc gaacatctaa aaagaaaagc aaacccagct
    19321 gtttcctttt caaacattcg ccacttattg tctggtagaa aaggctgtgt ctgaactttt
    19381 tgacacagtt cttttttttc ttatctgctg ttaatttttt gttaggattt ggcagaagat
    19441 aagacaaatc catcgatctc tagtaaaata ggcaaggaac tttttgtcgc ttgataagaa
    19501 aagacaaaaa aaggaggaaa cgaaccatga cattaacagc tgaagtcatt gtggacgtac
    19561 ctactatgca gaccgatcag ccattcactt atttggttcc ctctgaaatg gaaacagctt
    19621 tgcaagtcgg gatgcgagta gaagtccctt ttggtaatgg caatcgtcat gtccaaggat
    19681 ttgttatggc tatccaaaaa tcagaagaat cagctaatcc atctttgaag gcagttattc
    19741 gtttgctaga ccttgctccg gtggtcaatg aagaattact ttcattagct gattatatga
    19801 aaaaaattac ttacgcattc aaaatcactt gtctacaaac gatgcttccg agtgtgatga
    19861 aagcagaata tgataaattg atctatcctt tggcagacac accagaagtt caggtattgt
    19921 ttgatcagcg agaagtgatt tcctggaagg aagcagaaga agaaggcagc ctttctcaat
    19981 tgattcgctg gcgccaagag caattagtag atatcaaata tgaagtccat acacgaaata
    20041 aagtaaaaac gattcgtttg gtccgttctt tgttaactga aaagcaaatc gaagaagaat
    20101 gggcaaaatt acggcaaaat gccaagaaac aaaaagaact tcttttgtgt ttatctgaac
    20161 tgagccaaga agaaccaatt gcttatttca aaaataaagg aatcagtact gctgtcttga
    20221 atcaaggaaa agaaaagggc tggctggagt ttatagaatc agaacgttat cgcgaccctt
    20281 ataaagaccg agtatttgac caaacgacgg cattggaact gaatgcagaa caaaaaaatg
    20341 ctgtcgaaca aatcatcaca gcaggtcagc agcaaaaaga tcaggtcttt ttgctggagg
    20401 gaatcactgg cagcgggaaa acagaagtat atcttcaggc aatcgctgat gttttgtcag
    20461 aaaataaaac ggctattatg ttagtgcctg aaatcgcatt gactccgcaa atggtcgagc
    20521 gattcaaagg acgcttcgga gaatcagttg cggtcttaca tagcggactt tcccaaggtg
    20581 aaaaatatga tgaatggcgc aagattgagc gggaagaagc gcaagtagta gtaggcgcac
    20641 gatctgctat ttttgccccg ctgaaaaata tcgggctgat cattatcgat gaagaacatg
    20701 aatcaagtta caaacaagat gagacaccgc gttatcatgc gagagatcta gcaatttggc
    20761 gcagtaaata tcatcattgc ccaatagttc taggaagcgc gacgccctct ttagaatcaa
    20821 gagcacgagc tcaaaaaggc gtttatcagt tattgcagtt aaaccaccga gccaaagcag
    20881 ctgcacagct tccagctatt gagatcgtag atatgcgtga agagtttcag aatcatcgga
    20941 cctctacttt ttctgctaat ttgcaggaaa aaatacaaaa tcgattagac aaaaaagaac
    21001 aaactgtcct actgctcaat cgtcgaggat attcgtcttt tgtgatgtgt agggactgtg
    21061 gatttgttct tccatgtcct aattgcgata tctctcttac actgcatatg gatacacgtt
    21121 caatgcgctg ccattattgc ggacatgaag aacctattcc caatcgctgt cctaattgtg
    21181 gcggcaataa aatccgctac tatggaacag gtactcaaaa agtagaagag gaactccgag
    21241 aactttatcc gcaagctagg attttgcgta tggatgtaga taccactcga agaaaagggg
    21301 cacacgaaca gattttacaa aaattcggtg caaaagaagc agatatatta ttagggacac
    21361 aaatgattgc taaaggtttg gactttccag aagtgacatt agttggggtg ttgaatgctg
    21421 atacctccct gaatttaccg gattttcgtt ctagtgagca tacattccaa ttgcttaccc
    21481 aagtttcagg acgagcaggg cgagcggaaa aagcaggcga agtcgtgatc caaacattca
    21541 atcctcaaca ttacgccata gaattggcta aaaaacaaaa ttatgagcag ttttatcagc
    21601 aggaaatgca tgtacggcat cgtggaggct atcctcctta ttactttact gtgaaaataa
    21661 ccgccagcca tcctgaagaa caagtagcgg caaagaaaat ctttcagatt gccaatcagc
    21721 taaaagaagt gttaagccct caaagcttgc tattagggcc aacgcccagc atgattttga
    21781 gagtgaaaaa ccgatattat tatcaactaa tcatcaaata taaacatgaa ccgaatcttc
    21841 ctcatgtttt ggatgaaatt ttgaatggaa gccagaaaga acagcgacaa ggtctttttg
    21901 tggcaattga caacgaacca ttaaacttta tttaagaaag ggccaaatta tgcgctatcc
    21961 tatattgatc caccctaatg ataaattaaa aagaactgct cagccaatcg atgtaatcac
    22021 ggacgagaca atcgctttat tagacaattt atatgagaca atgattgcga atgacggaat
    22081 agggatcgct gctcctcaag taggacaaaa caagagaatt gctgtgatcg aagtagatga
    22141 aggcgagaaa tttgaactga ttaacccaga aatcatcgaa gcaaaaggtg aaagccttga
    22201 cgtagaaggt tgtctcagta ttccgcatgt ctatggaacg gtcaagcgag cagatgaagt
    22261 gaccgtgcgt tattatgatc gagacggaga agagatagaa gtgacggctt ttggctattt
    22321 agcccgtgcc ttccagcatg aaatcgatca cttagacggt atcttgttca ttgagaaaat
    22381 gatacagcag atcccggaag aagaacttga agaatatatg gaggaacatc ttgatgacta
    22441 agctagtatt tatgggcaca cctgcttttt ctgtgccgat tcttgaaggg ctgatagaat
    22501 caggttacga gatccaggca gtggtaacac agcccgaccg tccagttggg cggaaacgag
    22561 taattacgcc gacaccggta aaagaagcgg ctctgaagca cggtatccgt gtgctgcagc
    22621 cagagaaaat ctccggttcg ccagaaatgg aagaaatcat tgaattggtg cctgatgtga
    22681 tcgtcactgc ggcatttggc caattcttgc ctgaaaagtt attgcaagta ccaaaattag
    22741 gggcaatcaa tgtccatgct tctttacttc ctaaatatcg gggaggtgcg cctgttcatt
    22801 atgccatcat gaatggggag aaagaaactg gtgtaacgat catggaaatg atcaaaaaaa
    22861 tggatgcagg cggaatctat gcgcaagaaa gcataccaat cactaaacaa gatgatgtag
    22921 ggacgatgtt tgaaaagctt agccttttag gaagaaagct attgctagaa acattgccca
    22981 atattttgga tggacaaaaa ccagtacctc aagacgagtc agaagcaact ttttcgccaa
    23041 acatcacacg ggaacaagaa gcaatcgatt ggcataaaac tgcagaagaa attgacaacc
    23101 aagtacgcgg aatgcgtcct tggccaattg cattcacgac ctatgagggc atacgctgga
    23161 aattattatc ggttgaagta ttggcagaaa ctacagacaa agcacctggt acgatcatcc
    23221 ggaaagacaa aaaaaatcta tggattgctt gcggtaaaca gaccgtctta gccatcaatg
    23281 aattacagcc agctggtaaa ggaaaacagg ccgtacatga tttccttaac ggaagcggtc
    23341 agcaagttca gattggtcag caggtgaaat aatgaaaaaa atccctaaaa aaatgaaaaa
    23401 atcagtacga tatatggcat tactgacatt agagcgagtg aacaagggtg gcgcctactc
    23461 gaatcttttg ttgaatgaga tgatcaaaca aagcgagcta aatcaaaaag atgtgggttt
    23521 atttacagaa ttgatctatg gtacgattag ccatcagcga ctattggaat tttacgttgc
    23581 accattgatc gctaaagcaa aaaaagtgga tgactgggtc aagacgcttc tttatctttc
    23641 tgtataccaa ttagaatttt tagataaagt ccctgcacat gctattttaa acgaggcggt
    23701 cgaaatcgca aaagtaaaag gaaatcctgg gacagggaaa tttgtaaatg gtgtcttgag
    23761 gaattatcag cgtcaaggag caccagatct gcaaggcatt gctgatccta tcgaaagact
    23821 ttctgtcaaa atcagcctgc ctctatggat gactaaacga ctggtggatc aaatcggata
    23881 cgaagaaact gaaaagctcg gtttatcgct atatcagcct agccatgcta gtgcccgagt
    23941 agatacacgc cgactttcca gagatcaggc catcactgtt ttgcaagaag aagagatcac
    24001 agctgaaaaa agccaagtca gtccatacgg tatagtagcg aaaaaagggc atttagcttc
    24061 tagctcattg ttccacgatg gcgtcatgac gatccaagat gaaagttcga tgcttgtagc
    24121 gccgtctatg caaatcgagc ctgaacatca tgtcttagat gcctgtgcag cacccggagg
    24181 taagacgacg catattgctt cttttttaga tccgtcatgc ggtggagaag tggtcgcttt
    24241 agatgtacac gaacacaaag tcaaactcat tgaagataat gcagcacgtc taggtgtctc
    24301 ttctgctgtc tacgctcaaa agatggatgc tagagaagtc agtaaacagt tcgaagcaga
    24361 gcagtttgat cggattttag tagacgcgcc ttgttcaggt cttgggctgc ttcggcgaaa
    24421 accagatatt cgttataaaa aacagccgga tgaattagcg aatttaccaa aaatccaatt
    24481 ggaaatttta gaaagctgcg caccaacttt aaagtcttcc ggtattctga cttatagcac
    24541 ttgtacgatc ttaaaagaag aaaaccaaga cgttgtttct gctttcttgg aaagacaccc
    24601 tgattttgag aggattgatg ttttgaccaa tcctatgcta caatcatcaa tcgaagagaa
    24661 aatgttgact ttgtatccgc atcagtttta cacagatgga tttttcattt gttgtttgcg
    24721 caaaaagtaa tgaggtgaaa aaatgcagat tgaatatcaa tcagatgtag gaagacgacg
    24781 taacacaaat caagactatg ccagtgtatt taccagccgt tcaggtctca agcttgctgt
    24841 attagcagac gggatgggtg gtcatcgtgc cggagacatt gccagtcaga tggcggtaac
    24901 gaatctaggc gaagcttggg aaaaacaaga agctgttgac gatgaaaaaa tcgcccaatg
    24961 gttcattcaa tcgattcaag aagaaaatat gctgatttat cagcgtggac aagaacagcc
    25021 ggaatataac ggtatgggaa caacaatcgt agcggccgct ctgtcagatg actggttcac
    25081 gattgccaat gttggggata gcagagctta tttgattcgt gaagataaaa tcatgcaatt
    25141 gacggaggat cattctttag tcaatgaact agtcaaatca ggagaaatca gcgaagaaat
    25201 ggcagtgaac catccaagga aaaatatttt aacacgttct gtcggcatgc ctggaacagt
    25261 cgaagtagat gtttctactc atctttggca actaaaagat cgactgctgt tatgttcaga
    25321 tggcttgacg aacatgctgt ctgaagaaga aataggaacg atcatcaatc aagaaggccc
    25381 attgtctcat aaagtaactg agctgattga tcgtgccaat gaagcaggcg gagcggataa
    25441 tatcaccgtt cttcttatcg aatataagga ggacagtgta tgatcgagtt gggcaaaaaa
    25501 ttaaatggcc gttatcaaat cactggaaat atcggtagcg gtggtatggc aaatgtattt
    25561 ttggcgcacg atttaatctt ggatcgtgat gtagcagtta aagtcttacg atttgacttt
    25621 caaaatgacc agactgcgat tcgacgattt cagagagaag ctttagcagc aacagaactc
    25681 gttcatccaa acattgttag tgtttacgat gttggtgaag aagacaacat gcagtatctg
    25741 gtgatggaat acgtcaaagg aatggacttg aaacgatata tccaaacaca ttatcctgtt
    25801 ccttatgaaa cagcagtaaa tattatgcag cagatccttt ctgctatttc tttagctcat
    25861 aaccatcaaa tcatccaccg agatttgaaa ccccaaaacg tactgatcga taatgaaggt
    25921 gttgtaaaaa tcacggattt cgggattgct atcgctttat ctgaaacatc gatcacgcag
    25981 acgaatacga tgctaggatc tgttcattat ctgtcccctg agcaggccag aggaagtatg
    26041 gcaacgaagc agtcagatat ttatgcttta gggatcatcc tttatgagat gttgacgggt
    26101 agcgtaccgt ttgatggtga atctgctgta acaattgctt taaaacattt tcaagatgat
    26161 cttccatcta taaaagcact tgatccaaat gtgccacaag cactggaaaa cgtgatttta
    26221 cgggcaacgg ctaaagaacc tgcagaccgt tataagtcag ccgaggaaat gtctgatgat
    26281 ctttctacag tattatcacc agctcgtgca aacgaagaaa aatggcagcc tcatgtgatg
    26341 gataatgaaa caaaagttat cacacccctt acggaagatg caccaatgcc tgattcattc
    26401 aaatctatgc ctctgccaaa agacaagcca gaaaacgaaa ccttaccaga ggaggaagaa
    26461 gttctgcctg atcctaaaaa gaaaggacag aaaaaatggt ggctgatcct attagctatt
    26521 cttgctgttt taggaatcgg aacagctgta ttctttgctt ctggcgggcg aggagaagtt
    26581 gttgttcctg atgtttctga tctttctgaa gcttctgcta gagaaacact cgcaaaagcg
    26641 aaattagaag tagcaacaaa aacagaagaa attgcagatg acaaaatcga agaaggtaat
    26701 gtagtgaaga cagacccggc agcgggaacg acagtcaagc aaaaacgcga agtcactctg
    26761 tatattagta gcggcacgaa aaaaatcaaa ttagatgatt acagtggtaa aagttatgaa
    26821 gatgccgaag caagtttgaa agaattaggg ttttctgctg acttgattca aaaagaagag
    26881 gaattcagtt cagaggtaga aagcggaatg atcatttcgc aatctccatc agaagataca
    26941 gaagtcgatc ctaaaacaga cacgattact ttcacagtca gcaaaggacc agaaacagtg
    27001 actttgagta gtcatgtagg agaaaactac gaaactgttc gtaattggct tgttacacaa
    27061 ggattcaata tcaataatat tagtgctact tatgactata gcgacactgt accatcaggt
    27121 tcgatcatca gtcaggatcc agcctcgggt gaagttgtag cagaagaaac ttacatcaat
    27181 tttgtcgtaa gccaaggaac agagcctaaa ttgaatgata tctccggcta tacaaaaagc
    27241 gaagcacaaa gttatttagc tagtgtcggt gcagaatata tcgggcatga gacttccgaa
    27301 tattcaaaca cggtggataa agataaagtg attcgtacag taccaggggc aggaacgaca
    27361 attaccaaag gaatggttgt gaacgtaatt tactctaaag gaccagatcc agcgtcttct
    27421 agttctgaaa aagactcttc agacagttct agcagctcaa gtagctccag tagcacaagc
    27481 aactcttcaa aagaagaatc ttccagctcc acaagttcat cagctagcac ctcagaaagt
    27541 tcaaaataag tgctgaataa aatatatcag aagggaatgc gacattgatt tgttgcattc
    27601 t
//
LOCUS       DAZUMK010000027        27561 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000027 DAZUMK010000000
VERSION     DAZUMK010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.027"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(125..1069)
                     /locus_tag="ACG808_001124"
     CDS             complement(125..1069)
                     /locus_tag="ACG808_001124"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFS5046767.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1365..2309)
                     /locus_tag="ACG808_001125"
     CDS             complement(1365..2309)
                     /locus_tag="ACG808_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS5046768.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2530..3744
                     /locus_tag="ACG808_001126"
     CDS             2530..3744
                     /locus_tag="ACG808_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5046769.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3766..5781
                     /locus_tag="ACG808_001127"
     CDS             3766..5781
                     /locus_tag="ACG808_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFS5046770.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5864..7237)
                     /locus_tag="ACG808_001128"
     CDS             complement(5864..7237)
                     /locus_tag="ACG808_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFS5046771.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7263..7547)
                     /locus_tag="ACG808_001129"
     CDS             complement(7263..7547)
                     /locus_tag="ACG808_001129"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5046772.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7549..9564)
                     /locus_tag="ACG808_001130"
     CDS             complement(7549..9564)
                     /locus_tag="ACG808_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS5046773.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9743..10048)
                     /locus_tag="ACG808_001131"
     CDS             complement(9743..10048)
                     /locus_tag="ACG808_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFS5046774.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10137..11168)
                     /locus_tag="ACG808_001132"
     CDS             complement(10137..11168)
                     /locus_tag="ACG808_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS5046775.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11375..12250
                     /locus_tag="ACG808_001133"
     CDS             11375..12250
                     /locus_tag="ACG808_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS5046776.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12402..13244)
                     /locus_tag="ACG808_001134"
     CDS             complement(12402..13244)
                     /locus_tag="ACG808_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS5046777.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13336..15213)
                     /locus_tag="ACG808_001135"
     CDS             complement(13336..15213)
                     /locus_tag="ACG808_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS5046778.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15210..16631)
                     /locus_tag="ACG808_001136"
     CDS             complement(15210..16631)
                     /locus_tag="ACG808_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS5046779.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16634..20023)
                     /locus_tag="ACG808_001137"
     CDS             complement(16634..20023)
                     /locus_tag="ACG808_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS5046780.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20280..21671
                     /locus_tag="ACG808_001138"
     CDS             20280..21671
                     /locus_tag="ACG808_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5046781.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21744..22391)
                     /locus_tag="ACG808_001139"
     CDS             complement(21744..22391)
                     /locus_tag="ACG808_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5046782.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22399..23085)
                     /locus_tag="ACG808_001140"
     CDS             complement(22399..23085)
                     /locus_tag="ACG808_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5046783.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23091..24164)
                     /locus_tag="ACG808_001141"
     CDS             complement(23091..24164)
                     /locus_tag="ACG808_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5046784.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24386..25060
                     /locus_tag="ACG808_001142"
     CDS             24386..25060
                     /locus_tag="ACG808_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5046785.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25065..26405
                     /locus_tag="ACG808_001143"
     CDS             25065..26405
                     /locus_tag="ACG808_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFS5046786.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26554..27540)
                     /locus_tag="ACG808_001144"
     CDS             complement(26554..27540)
                     /locus_tag="ACG808_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFS5046787.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8024 a         5225 c         5008 g         9304 t
ORIGIN      
        1 tcggaccgat taattttaac cattctgttt atttgcttac ctattagata tcaataaaaa
       61 actgcttaac agctatttca gctgttaagc agttttagga tttttagttc atattcattg
      121 tttcttataa tacagtgttc aatacttctc ttaaagcact tgttgatttg ctgaacattg
      181 ctttttcttc ttcgttgatt gataattcaa caacttcacg aacaccattt tcgtcaacga
      241 ttgatggcac acctgtaaag atatcttttt cgtcgtattc accagttaaa taagcagata
      301 caggcaatac agcttgttcg ttattcaaga tagcttttac gatacgtgca gtactcatac
      361 caataccata gtaagttgct tttttacgat caatgatctc ataagcagca ttacggactt
      421 tatcagcaat cacatctaat tcatcttggg caatacgatg gtctttttct acaatttcaa
      481 acactggttt accaccgaca gttgtatgtg accatgcagc aacttctgaa tcaccatgtt
      541 cacctaagat atagccgtgg acgctgcgtg ggtcaacttt taatttcaat gcgatttctt
      601 tacggaaacg agttgtatcc aatgttgttc cagtaccgat aacacgtgat gttggcagac
      661 cagattcgtt ccaagcgata tatgtcaaaa tatctactgg gtttgaagca acaacgataa
      721 taccgtcgaa tcctgatccc atgatttctt ttacgatttg gcgcatgatt gacgcatttg
      781 ttttaactaa atctaaacga gattgaccag gtttttggtt gataccagct gtgataacaa
      841 cgatatcagc atctttacat tcttcatagc cacctgacca tacagccaca ttttcatcgc
      901 cccaagccat accatctaaa aggtccaatg cttccccttc tgctttttct tgattcacat
      961 cgatcaatac taattcatta gagattcctt ggttgatcat tgcataagcg atacttgttc
     1021 caacaaaacc tgtaccgacg attactactt tacgacttgt ttttttcatg aataaacagc
     1081 tccttttcat aactatttgt tactttcatg aaaagtatag tcttgtttat tcattttatc
     1141 aacatgttaa ttataatagt tgttattata atttttgatg gaaacgaatt cctgaatacg
     1201 cataacagat aaatgacaaa tgtttacacc tatatcccct gataatacaa ccaaaagttt
     1261 agaactgaaa ataaatccaa aaaaattcgc tgcaacgata taataagaca gcaatcgcac
     1321 ctaaaacaat tttccttcat atgctttagt gaaagatttt cttcttaatg tttatttctc
     1381 cattcattgg gagtacatgt tgaatacttc ttgaataatt tgaataaaga agtcgtttgt
     1441 ttatatccga tacgattagc gatttcttcc aacgaaaaac tcgtttcttg aatcagttcc
     1501 aacgcaaagt cgaatttttt ctttagaacc agttcttgaa aggaagcatc aaaatacatt
     1561 ttcaatttat tactaagata gaccggattg tagccaaaat gagctgctgt ttcttttaac
     1621 gtacaagaag taaaatgctt atcgatataa tctaatactc taagcatctc attttctgcg
     1681 catccatctg ctgatgaaaa gctttgttgt tctaataaat gagtcaattc gatcagtaat
     1741 agtgatgtat agagatttaa cgaacggatc ttatgactgt atgtatcaaa ataaatcaac
     1801 agcatatttt caatcaaatg cgtcaaacga cttgtttttc cgccgcgaaa atgaatgaat
     1861 tgtgtatgtt cccctttttc ttttgatgca tttatcaaaa aagaagaaag tagattattt
     1921 gtgcttgcca aatgattcaa tatctctgta gataaggttt cttcttttaa taagatgttg
     1981 attaaaatat cctcttgccc tgtgtattcg attctttgag tagcttcttt atcaatgatc
     2041 accacgtcac tttcttccat ctctatcctt tctccattaa taatttgtaa acatgtaccg
     2101 ctatacatat aattgatttc aacaaaatca tgtgtatgct cgggaacttc cgcatatcga
     2161 cggtgtttac tgatatgaat atttccctgt tgaaatagat cccctcggtg gatttttggc
     2221 ggtgtctgaa gatggtaagc taagtttaaa tcattgatat tcattccttg ttcacgctgc
     2281 agagtttcga ttgattcttc gttacgcaaa aaaaatgtct aattcagcta gttccaattg
     2341 actccctcct ttttgtaagc ggtttctttc gcttattagt tcacgctgta ctgctattat
     2401 atgcaaagct atttttggtt tcataaagac tttctatggt tgggacatga acttcctgtg
     2461 attccgctat tgagagaata accacttcat actatatttt tgataagcgc ttacaaaaat
     2521 gaaaggaaca tgcttatgaa taaaaagatg atgaaatttt ctctgctgtc agtctctctt
     2581 attttgactt ctgctggtgc agtcacgtct gcaattccca gtatgtcagc tacttatgcc
     2641 acgcgttctt tatctgctgt tgaattatta acgactgctc cctcttttat ggtcactatt
     2701 tttgttttga ttagtagttt tattgctagg aaaatcggac aaaaacaaac tgtgatcatc
     2761 ggattagtga ttgcatcgat ttgtggtatc ctccccctct tctcacaaag ttacagtatt
     2821 gtcttagctt caagagctgg actaggtatt ggctttggat tgatcaattc tttggcagtc
     2881 agcatgattg ctgcctttta tcacggagat gaacgagcct ctatgattgg ttttcaaagt
     2941 gcatttcaag gatttggtgc agctttaatg acatttgttg caggacagct agtacagttc
     3001 ggttggcaat ataccttttt agtctacgcc atcagcttac ctattcttgt cttgtttgtt
     3061 ttgtttgttc ctacacctga aacaactgaa cttcaatcgc atggcacagc cccaacaaga
     3121 caagtaatca aaacgaatcc taaaatgatt agttatggta tttttctctt gctggtcatc
     3181 gtggtatata atgctgtatc gattaaatta gccacagtcc tgacgactta ttctctagga
     3241 aacgaaacta atgcagcgaa tatcttaaca atcatgcaat tggcaagtat ggctgccgga
     3301 gtttgttttg gctggcttca gacaaaagca catcgctata tgcttacact ttctttacta
     3361 ctgatgggaa tcggctttgt attgattgcc actgtgataa acctgtatgt gattgggttt
     3421 ggtgcgatcc taacaggaat ttctttttcg ttattcattc cttacttatt caaccaagta
     3481 agtatccaag cgcccaagta cgcggagagt ttcaatactt caatcttatt ggttggcgct
     3541 aatttaggta ctttcattgc accatatggt ttgctctttc tctcttttgc ttctcttggg
     3601 agtaaaacac ttgctgtttt cgtcaatggc agtatcttgc tgttgattag cgcgcttatt
     3661 agccttctag tagtacacag gacgaataga aagacagtga taagagtaga tgaagaaata
     3721 aaagaacaat tgacaacaaa ataaatttga tggaggtcgt gtaaaatgac aaatgaaaaa
     3781 cataaagata gatggctttg gtatcctgga gactttgaga tccgccatgg actgctacaa
     3841 aatttccaac gggaagaacg aggttttgac tggccagcgt actggtacat ggatgattgc
     3901 catcggaacg tcaagtttaa gagatattac tttcttgatc aaccttccat gtttaaagtg
     3961 acgatccaag gagttgggta tgtagaaatt aatggacaaa aacatccatg tggaaaatgg
     4021 ctgacttgtc cagctggtaa agcaaaaatt cgtatctttg taggacatac atcagggcta
     4081 ccagcgatgt ttatcgaggg agacgaagta aaatcagata ttggctggac agcaagtaat
     4141 tttattgaag aatatccagc tggctggagt ccgctttatg tcgatatcgc aaaagatcca
     4201 aatcaaatct attatcaaaa agaatgcgtc catccagtca ctgaaacgga gattggcgga
     4261 ggaatcttat tcgattttgg gcgagcagtg aatggctgtg tagaactaat atctctggat
     4321 gaccatatcg aatctatcac tctatgttat ggagagtccg atgtagaagc tatggatatc
     4381 gattattgct actataagca aataggaatg acaagaaaaa tgaaagctcg taaacgcgct
     4441 tttcgctatt tgtttatacc agaggtttct actggaatgg ttcaacttca agtttttcat
     4501 gaatttttat ctctttcgaa acaagctgct ttttcaagcg attcttcttt actgaatcag
     4561 atctggtcag tatcagaaga agccttcaag ttatgtagtg atttgttttt tatcgacggt
     4621 atcaaacgag atcgctggat ttggtctgga gatgcgtatc aatcattcat gatcaatcaa
     4681 tatctttttt ttgacgagga aatcaataaa cgaacgatat tagcattaag aggacaaaat
     4741 gaaatcaagc agcatatgaa tactatcgtt gactattcga tcttgtggtt gattggtatc
     4801 gaaaaccact atatgatgac gaaagactat gaatttttac gcatcgtcta ccctaagatg
     4861 caatcattaa tgagatactt gatggagcaa acgaatgaat tagggtttat ttatgggaga
     4921 gcaaaagatt ggatatttat tgattggtct gaaatggata aagaaggaac gcttgctgca
     4981 gaacaagtct tattactgaa agcctatcat tccatgatca cttgcggaga tatcttagct
     5041 gaaacatatg gagagaacga aaattatcat actcatctct atcaagaaaa atatcaacta
     5101 ttgaaacaaa acctatttga ctacttttgg gatgaagaac ttggcgcttt tatcgattgc
     5161 tatgaatctg ggagaagaaa tgtgacgcgt catgcgaata ttttcgctgt tttattcgat
     5221 tttgctaaca aagaccaaca gcaatcgatc ttaaagaatg tcttattgaa tgacactatc
     5281 acacagatta ctactccata tttcaaattc tatgaacaag atgctttatg caaactcgga
     5341 cagacgaata tcgtctacca aacgattctt gattattggg gagggatgtt atctcatgat
     5401 gcggtaactt tctgggaaga atacgaccct actcaaacag gaaatgaaaa gtacagtatg
     5461 tatggagatc cttatggaaa aagcttatgc catgcatggg gagcaagccc aatctatctt
     5521 ttaggacgct attttatcgg tttgtatcca acaaagccag gttatgaaga atttatgata
     5581 caacctaatc tagattcatt tgaaacactg aattgtacgc tacctatcaa agatggaaag
     5641 atttcgcttg aaaaaacaaa agataagatc gtgattactt ctgatagaac aggcggaaca
     5701 gtgaaaatga atggtaaaag ttatcctctt ctagcaaaac agccacttgt cttaacaagc
     5761 tattcaaaaa taaatgcata aaacaaaaaa ctgcttgcga catatcagaa gactttaaat
     5821 cttcttctga tattgtccaa gcaattttta ttttgaagtt tgcttaattc tgttctttag
     5881 aaacaatgtt tgttcttttt ccggcaaaaa gaaagctaga taatacgaca agtaaaaaga
     5941 aagcgatgat tccaactgtc aaggctattt gaccgtattc tcctagtttc ccaagtacaa
     6001 gtcctggtac tagataatca gtatcggcaa atgtagaaga agcgatttta agatctccta
     6061 atactggaag gataaataat ggcagccaac taatcaataa gctattgaaa gcagaaccta
     6121 ataccgcccc tcttctaccg ccagtcgagt tgccgaagac accagaagca gctccgcaga
     6181 agaaatgtcc tactacccca ggaatgataa ctgtagtttg caagcctatc atagcaaaca
     6241 tggatagtgt tcctacaaag aagctaacaa aaaagccaat taatacagca ttaggtgcat
     6301 atggaaaaac gataggaaca tctaatgctg gtttagaatc gggtacgagt tttttagcaa
     6361 tcccttggaa tgctggaacg atttctgcca acaccattcg aaccccttga aggacgataa
     6421 caaacccagc tgtgaatgtt ccagcttgga tcagtgcaaa gattacagcg ttgtctccat
     6481 tgcttagttc tctttcaaca aatccaggac cagctaacaa agcaagtgcg acatatacga
     6541 tcgacatcaa gagcatgatc gatacagtag aatctcgcaa aaagctgaaa cttttaggaa
     6601 tctttatgtc ctctgtagaa cggtctttgt ttccaaaaaa ctttccgatc catccactag
     6661 aagcataacc gatatttccg gtatgcccta acgcaacttg attgtttcca gtgatgttct
     6721 tcataaacgg ttgacaaaga gcaggcgtaa tagttaatag tgttccttcg aagaccccgc
     6781 ctaataagat catccacaaa tttgcagtaa accccgcgct tactaaaatg actgctgtta
     6841 gacaagaaac ataaagcatt gcttgacctg taagaaaaat gaatttatat cttgtgaatc
     6901 tagcaagtaa aatattcacg atcatcccta ctagcatgat caaagcagta ggtgtaccgt
     6961 attgttgcaa agcaatcgcc actactgctt cattgctagg aactactcct tgagtatgca
     7021 gagctgtttg gaacatcgaa gcaaaagggg ttagtgaatt actgattaaa ccagcaccgc
     7081 cgatcagtac taggaatccg acaaatgttt tgatagttcc ttggatcact tctgaaaatt
     7141 tcttcttctg aagaacgaga ccaagcagtg caatcaaaga aacgaggatg gagggctgac
     7201 tgacgattga cacaaacaaa ttcaacaatt tatccattga tagttcctcc cttgaatcat
     7261 ttttattctt ttgacaagat cttcgttact ttttctttta cttcttcgct gtcaatgatc
     7321 gattgcaaag gtacgagctt ttcatcaggt atagaagtta gcgtatcttt caaagaatgc
     7381 tcaatgaaga aataatcagc catatcgcta gatacacttc caacatccgt atgacttact
     7441 tcaaactcgt ccacatctgc tccaagatct gcaagtgctt gtttgatgtt catctctacc
     7501 ataaaacttg ttcctaatcc actttgacat actgcaaaaa ttttcatatc attcgtctcc
     7561 ttttcttatg atcgctaata tttcttcttt tgttgatgcg tgtaataaat cttgaagatt
     7621 ttgtttatta gaaagtagct ttgttaaatt tgccaaagcc cgaagatgtg cctcattatc
     7681 gatagcagct aaacagatga acaaactaat gggatgggct ggattatcaa gcagatccac
     7741 aggttgttga agtttcagca aagacatccc tacttcgttt acgccatctt ctggacgagc
     7801 atgaggtaat gcgatgtgat cgccaatatg gataaacgca ccatactgct ccacgcgctc
     7861 gatcatagct ttgatataac tttcttctat tttattttct gataatagcg gctgtgcggc
     7921 aaatgtgatt gcttcttgcc agttttttgg ttgatccgat aattggatca tttcttctgt
     7981 taataattca gatagcatag gacgttgatc ctccttcttt tctaaaagtt tgttcatttt
     8041 acgatttaat acttgataga gcttttcttc gtctacgcct tcttttaatt cgatataagg
     8101 cagtagcgcc gacaacaatt cttgggcaga aggcattgaa aatcctggta tcagccaatc
     8161 tctttgtact tgattgatca gctgattttt ttccaagtca gtcatgattg gatgcagaac
     8221 atagagagac tttttcttat cagtaagtgt gagaggtaca gtagaaaaaa taatatcgta
     8281 agaagtttcg tcaacctcgt gtaagcggtc agctgaatta gtctctgtaa aaaagatcat
     8341 cgggaataat tgctgaagtt cactttttaa gatcaatgaa gagctaatgc cacttggaca
     8401 gacgatcacg gctcttattt ttctatttct ttcttctgcg tccgcttttc tgatctctcc
     8461 gccaaatagt atagtgaaaa aaccaatctc ttcttcaggg atgctcttcc ctattcgtct
     8521 ttctaacgga agtaatgctt ttcttgtcat ttcgtagatg gaagcatatt gatgcttaat
     8581 ttgatcgatc atcacattag gtaaataaaa agaataggaa atacgaaaat aggcagggac
     8641 caagtgataa aagacattca tcaataattc acgaaacgaa gcaaattcta cggccatcag
     8701 gaattccatg cgatggatga cttctgaaga acattccagc aaaaaatcaa gtgcaggctc
     8761 ctggacatct ccttcaacga tcgatgctaa gcaaagagag aaaaatataa tctccgaatt
     8821 ttgatgttcg gaatagccaa ggccagtcaa tacttgtcga ctaatgtcag tgactgcaag
     8881 agaagccaac agttcagcat cttttaccag ttccatggat gtttttccaa ttcgaatggt
     8941 tgtcaaactc aaataataag ctaattcatc cattcgattt ggtgcgaatc gaagatctga
     9001 tagtgaaatc gttttatcta gcaaatctct aactttggcg taataatcaa tggaaagggt
     9061 tgcggttaaa tacgtgagcc caaatcttcc agcaagtgac tgcatcaaac gagcagtgcg
     9121 attttttgct tctcttctta atattgcttc tgaccctagc agcctaaaac cagttttcct
     9181 agtatacgac agctgacaat cttccacttc taattcattt cttatctttt tgatatctga
     9241 caggactgtc cctttagaaa cctgaagaaa atcttgaaaa tgaaaaacag aaacagacgt
     9301 tgcagtcgaa taaaccatca aataaagaag atctcttcgc tcttgttcgg tatacacagc
     9361 ttcaaatcga gtaatatcca aaaatttttc tgcccagtcg attgctcgga atttctttat
     9421 acggatacgg gtatcatcca catattcggc aaaaggagtt aattgtttga tcgcctcgct
     9481 tgtatttact tcgatgatct ctgctgcttt ttctagactg atttctcttg gtgttttcag
     9541 gagataacta attttgtagt tcacagcgct gttcacctcc tacttatatt ctagtatggt
     9601 agcgctttta aatcgagggg tgtttggtac aacttacagt tggatttttt ttacgactgt
     9661 accgaacgat ttctatcgaa tggatacaga aaaaaagcag gtgaccaaat gtctcctgct
     9721 tttatgaatt gatttcgtcc gatcaatgat gatcatgttt attttttgag gtcagttctt
     9781 caaatttttc tttgacatct tcaaccgctt tttcgatatc tttttcaaag tgattgggct
     9841 cattaggctt tgtttcctct tctacagaat ttgaaccaga taaagctgtt tctttatttt
     9901 ctgtttcacc agagtttgtg ccagctaatg attcttcttt gtcttctgtt tcaccagaat
     9961 tagttcctgc taaagccact tcttcttctg ttttaggggc cattgaacct cttaatcgtt
    10021 tgtgtttttc accgtcataa tttgtcatgt tggttcctcc tagtagttct atactttttc
    10081 atcgtacaat aagacagttt atgcgtcaaa aaacaaaccc gattcttaca aatatgtcat
    10141 atttttcctt ctgattcttg aatgcttttg attccttctt ccaaaatttt catttgtaag
    10201 attgtttctt catctttgat gactttttct gcgccattaa ctaatgtctc gtaaatccct
    10261 tcgtagacac ggctgtaatc accaacctcg gaaattactt tttcttcatg ataactccct
    10321 tgttcatcta tataggtcaa tacaccataa tgttcgggca aatcgatacc aaagtcttca
    10381 tgatcaggca tgtaaaacat tttcagatgt tcttcttgtc gatcttttgt ttgtttcacg
    10441 aacatccctt tttttccgta aacgacgaaa cttggacgtt ctttgatgcg gaaataacta
    10501 gattttatcg aaattttcat atttccatag aaaaaatcca gatcgaagta atcattcatt
    10561 cgtcctttcc ctaaaagttg acgtacatca tagctgatat catctggttc gccgaaataa
    10621 gaaagaactt gatctactgt atgacaagca tgtccatata aatagctgtt cgcagtagaa
    10681 aattcatgcg tattttctgg tacttccggt ctgaaataat cataatgcat ttctacttct
    10741 aaaatatccc ctaattttcc gctttcgatt actttttgga ctgtcaagaa gtcagaatcg
    10801 aaccgtctgt tttggtaaca ttggataaat agaccttttt cttttgctaa tgcaaataac
    10861 tcttctgctt cctttgacgt ttctgcaaaa ggtttctcta ctagtacatt cttaccatgt
    10921 tcgagtgcca tttttccata ttcataatga aattgactag gtgtagtgat gacaactaat
    10981 tggatctcag gatcatccca aagatcagac agctgatcag tatattcgat gtcttcgaat
    11041 gttttccaca ctggttcaga tggggaaaaa atttttttga tattgatttt gttcgtaagt
    11101 tttcttgaaa aaggcaaatg atagcgattt gtactctttc cattgccaag ataagcgatt
    11161 gtaagcatga gtggtgcctc cttttattga tgttttcagt atatcctaga aaagcgcaga
    11221 aagtatttat aatagctatt ctggtaaaaa agttcagaaa aacacttgtt atcattcggt
    11281 ttggtgaaat taatgttcaa agtaaggata aaaatcaaaa agatagtagc ggaaccaagt
    11341 gtttatcagt aagatagaat ttggaggtca gatcatgctt attgaagaaa aactaacaaa
    11401 acaagagttg tttaccacga cagagaaacg gattgtcgat tacatccgta ggaatatcga
    11461 agctgcagtc tacatgacga ttgaagaatt agcaaaagct acgtatactt cccattcagc
    11521 tatcattcgt ttatgcaaaa aaatgggatt cagcggattt aaagaattcc gtttcgagct
    11581 ggggcgggaa gttcatcaat tgattgctca gttcaatcaa acgattgatc ctaattttcc
    11641 tttttcaagt gaagatagca ctcagcaaat tgcaaagaaa atggcggatt tatcgatcca
    11701 gtcaataaaa aaagcacaat tgcagattga acatgaagat ttagatcaaa tcggtaaagt
    11761 attgaacaag gcacagcgaa tctttctttt tgcaaaaggt gattctcaaa tcacggcaag
    11821 gaaattccag aataaaatgg tcaagttgaa taagtttttg atcatggctg aagaatacag
    11881 tgattcttct tggaatgcag ccaatttgac tagcgaggat tgcgccattt ttattagcta
    11941 cagcggtcgc atccatcatt atgaacgaat aatgacctat ttgaagcatg tagaggcccc
    12001 taccttgctg attaccggaa atcagcattc agaaatggcc aaacaggctg atatgtgtct
    12061 tgttatatcg caggaggaat atgattttgc taaagtagcg actttctcct ctcaaattgc
    12121 cttcgattac gtattaaata cgttattctc agtgatatat tctcaaaatt ttgaagagaa
    12181 tatccgcaac ttgaaaaaca aacaagaatt gatgcaaaaa ggccttctta aagaatatcg
    12241 gaaaacttaa gaaaagatcc tcatttagta ataagaaaga tagtgtttga taggctctct
    12301 ttgatatcat cataaaaaca ggctgaagtt ttctttttca aacctcagcc tgtttgttta
    12361 cggaacgatt cgaatagata tattatagaa atgaggaata gctaacttct gtttcttatt
    12421 ttgaacgatt gacgaaaaag atatataaga aggaggaaaa tcagtataca tgcagctaac
    12481 aatccataca ggaaaaatcg tttgttctcg gatactttct gtacttcttt ttgaacttct
    12541 tttgcatgat aaggaacccg gtgccctctg acaagtaagc gatgactgtt gaccatataa
    12601 ggtgtacaag ttaggagtgt cacgaaatcc tgcccttttt cgattctcag atcttttgta
    12661 tcagtcggtt ctaccgtctt gatctgatcg acttgataag cgagtgtccg tccattgatt
    12721 tcgatataga aagaatcacc taaagtcagt ttcggtaagt ctgtgaacaa ctttgcttga
    12781 ggcagtcctc gatggctgga aataacggca tgtgtatttt ctccccccgt aggataagac
    12841 gttccttcca ataatgaggc acctttttct agcagcacag tattcgtttc atcaaaaatt
    12901 ggcaaatgga cattgatctc agggatcgtc aaaataccga tcgtatgttt ctcaaaatag
    12961 gttctatcct ttttcgcagg ttcttttttc ttagtaaagg gatctgcacc tggatttcca
    13021 ccttcttttg ctaattgctg gtttttcttt gtcatgtttt cttggatttt agccatgact
    13081 tcttcatttt ctttgaccgc ttgttgctga taatgagaga tgatctgctg atccagataa
    13141 ttattcagcg cgtcgctaac aaatggataa gctagtatcc ctattcctga gagaagcaag
    13201 aaaagcatca gcagatctag aagtttcctt ttctttggtt tcatttgtgt tctccttgtg
    13261 atgactgttt gataataaaa gaaaaaggag catttgcgta atccgctgct cctttttcca
    13321 attcttgcaa aaagactaaa tctgactgtg cttgcgtcta gcaaagtaca gtccagcaat
    13381 cagtagaagg actactcctg caccgatata cacatagatt cccttaccgc ctgttgaagg
    13441 aagtgtacct ttgtgtttat ttggtatttt ttcaggagaa atcaactgtc ctgctgttac
    13501 ataagattgt tcatcgatag taaatgctac acggtttgtt aatggaacat atttttctgg
    13561 cgctttcgtt tcttccagat agtacgtgcc atattttaga cctgtcacat cgattaaacc
    13621 gttacttgtg gttgtaaaaa ccgttgctga ttctttcgcc gatacccagc tgacggcttt
    13681 tgtggatggg tcgatcgata aatatttcgc tgtgtcacta tcttgatcac gaacgacgaa
    13741 ttctgctcca gcaagtgttt ggtctgatgt aacgtcacca tctactttaa caaatcgttt
    13801 gcccccagta acgacatcga ctgacggtgg tgtttgatca tttgtatgcc cgttatcgac
    13861 atttgcttgg ttagaaaatc ctttggttgg atctgctttt tcgttcaaat gcatatagta
    13921 aacgaatttc aatgtaccgc caggagttaa tgaaggaata taattcggat caactgaaac
    13981 cgtgaatccg tctgtttgct cagtgacaga ataatttact gggtcgattt ctttatttcc
    14041 atcatataag gcataagcat acgttcctga agaatcatta tcaaatgtta aagcagcgtc
    14101 atgagtatcg ataagtttga atgttgtgta cttgttttgc gttccttctt tatcagcgat
    14161 acctaatggg atattgacag aaatctcata ttgaattttt tcaccgattg cgacatcttt
    14221 tccattcaat tgaggtgttg ttttatcaac tttagacgta tcatttttga tggtcttttc
    14281 tactggtgtt tggctatttg ccaggatctc aaaaggcgtg attgtttgct tttcaatcat
    14341 ttccgcatta tcaggagctt ttacttcttc taaattatat tttccaactt ctaaatgatt
    14401 aacgatcaac tggccttttt caatagatgc ctcggcaaag tcattgttgc cgatgtcata
    14461 agaatgacca gtaatgaaat gtttggcttt ggtttgatca gttgtccaag tgtacaatcc
    14521 atctgtgaca ctttggatgt attttttgac gcttggtgtt ccttcttctt tagaaataat
    14581 aaattctgct ccatttagtg cttcgttttc ggcagtaccg atttttgtaa ctttcaacgt
    14641 tccatcatta ccgactgtat ttttagggta gagatggata gtatctagtt cttctgtccc
    14701 gtatttataa gagccatctg cttgtttgat catctcatat acagggaaag ctaaaaccat
    14761 gtttgcggca gctgacactc cgtcttttgg ttcttctttg atcgtgtaga ctgcatcttt
    14821 cccattttgt tttttaggta aagataaagt gacatttcca tcagcatctg tcgttcctga
    14881 agcaactggt gtaccaggag tcaaagactg gactgcttgt tttgctgcat ccacggacgc
    14941 tcctttatct cgttgcgcat aaaattcttg agtgacgtta taaactgaaa atgaaatatc
    15001 ggctaatcct tggtattgat cgaattcgct catttctttc ccgctgtttt ggattaaagg
    15061 atcgggtaaa tcagtcattt tctttttatg aaggatgact tgagctgctg tctcctctgc
    15121 aaacactttt tttgcgccga agctgtttgt gagtaatggt aaaataagga aaaggactcc
    15181 taacataacg tttatttttt tatgattttt cattttttca ctcctgttct ttttttgata
    15241 aacaaccaga ttgcaaagct tgcgaatacc ataccaacaa cgcctaaaag tgtatttttc
    15301 gtttcgtttg tttttggaag aaatggacgg ctattttctt ttttattgac tactttcaat
    15361 atactgtccg cctctttatc ctcagaaaga aaagttgaaa tggtaaacgg aatttctgtt
    15421 tcacttaaaa tataaccaga aggtgcttcg atctctttta atcgataact tcctgttttc
    15481 aacccggaaa ttgaaaaagc gccattttta tcggaaatca ggtgaagcgc atctgattcg
    15541 ttctttgtcc acttgtatcc gttggctgtt tgctggaggt aatttcctgc tttgttttta
    15601 ataaggaata ctgcgccagc taatcgttta tctggtttat ttgtctcgat tttgacaaat
    15661 tgcttactcc ctgtatatac agagactttc tttgtactga caccaaaacc aaaatccagt
    15721 gttccgttgt tgttgatttc cttgttcgcc tgtgctgtac tgcttagctg catctgataa
    15781 gaaatggtca gtttttttcc agcatatttt tccaacgaag ggatcgaaaa taaaacatca
    15841 aatccgtgag gtttcttgtg tatgacaaag ccttctgtca gcttttctcc attcgatgaa
    15901 atcgttaaac tattaggtaa aaacgttaat gcttcatccg cactatctga caattcgaac
    15961 ttttgatagt caaggatatt tacaggaatt gtcgtatgca aggaataagt gattttttca
    16021 ccaatcgtaa aatcatttct aggctcttct aataccttct caaaaggtgg atcagggtag
    16081 tcgttttctt catttttagg atacaaatgg atagttgaaa gtttttgatt gttttgtcca
    16141 tgtacaggaa gaacaactac catattctct gccttttctt tgacgacttc tggtactttg
    16201 gattcaatga aaagatacgc tttatctctt ttttcttgat ctttagcggc cagagaaaaa
    16261 gaagcgatac catcttcgtt gttaagagtt gtagttgttg cttctgcggt aaacataccg
    16321 gaagacgcac cgttttttgc gatctctgct tgtgcttctt ctaccgtttt gcccttttcc
    16381 cgtagatggt aaaaagaatc tgtgacatca taaacttgga atgtgacacc atttaatcct
    16441 cgatacgttt gtaataaagc tttttcttgt ccgtcatttg gatgattttt cggcagttgc
    16501 ccgtttggga aaagcaattt gtgtaaagta atctgaacca tttctgtttc tgtttctgtt
    16561 gctacctgag taaaagctgg tttaaatagt aacaagaaga gacacatact aagccaacca
    16621 agttttttca tatttagctg ccccctcttt cttttttcaa aaaaagatac caaatagaaa
    16681 acgcacaacc aatcatccct actagataga ttcctaaacg gccaattcct cccgtttcag
    16741 gtaatggtac ttttgcctga ttcgtgatgt ctaaagaaat ctggttgttt ttggctcctg
    16801 gtgacagaac agattcatga tcttgtccat ccacttgaat tgacccatct tcgtgtataa
    16861 ctatagtaac tggctctttt agaccttggt atccttctgg tgtaaaagtt tcggtcagcg
    16921 tataagttcc aggttttaaa ttttcgaata gaaaggtatc tgtttcttgt ccatcttttg
    16981 gcgattctaa gtccatttct ggtccctgca gtctgaattt tgctcctttt aatgtctgtc
    17041 cgttgtcttc ttttttgtgt accgttagat caaacgcttt tagtcgattt tgatgagtga
    17101 gcgtccatac attatttgtc atctcggtat tttttcccgt gtaaatgatt tttccatatt
    17161 tatcgatttg gaattcataa tttcccggcg aagtatcata tccggcagga ccagctgttt
    17221 caacgactcg atatttacca ggtgtgagtg aatcaaaact ggcaagacct ttttcattcg
    17281 ttgtttggct gtcaattggc tgataagtac catttgcttc ttttttctgt agggaaaatg
    17341 ctgctccttt aagatttatc tctttgtccg tcccttgcat cgcatactta cgtactgcta
    17401 caggaacttc aacaaaagga ttttcaatag tcaactggat cgtatcatcg gaagtagtga
    17461 ctgtttttcc atcaatggtt accgtaccat cagaagcgat cttgatttcc caagtagtct
    17521 ttttgcttaa tccatgccct tctggagctt ttgtttctgt cagcgtatag gtcatttctt
    17581 tttgcaattt gacattttct ggaagagaat aggttccgtc gccatgatct gttagtaaag
    17641 tatcaataga atctcctgtt aatttgaaaa cagcgccaat aagatccttt tcacctgtag
    17701 aggaattttt cgttattttc aagtttaacg gtgtgaattg tttagtattt ttccatgtca
    17761 ttgcatctat ttgttgagaa tcgtatcctg gtacaggtaa ttctttgatt gtttggtaac
    17821 tgaatgcttg accttgatta ttgtattgag gtagtgataa tatctcttga taactttctc
    17881 cgctatttgc agataatttg tcaatgtctg cacgttccca cgtatttgtt gtatctttag
    17941 ctggtttaat gattcgaata tatccgtttt tccaagctgc agcatttgtc gtatgttccc
    18001 gttgaatctc aaaagtaact tgatctggac gatcagctag attgttgtca aattcttccc
    18061 ataacttttt gatgtgaaga ctgactccgg gagctttagc agatggtata ccgaattcag
    18121 ctaattcatt ggtatcaatg tttggctgga aagttgtcct gccgttcatt tgataccaga
    18181 aatttggatg gaagtcctca ttttctgttt ggattctcac ttggtaatgg atttggattt
    18241 cttggtcttt tcctaaatag atctgattgc tcttgatggt atttccttct atggaaatag
    18301 atggagtgac cgttgtagga cttgtcccca cactcttgac tgaaagtgta ccaggctgat
    18361 aaatgaatgg ttctgcgatt ggatcattta tttgtccatt gcttacagtt gctgagatct
    18421 gtacagcttt tttggctagg tattcagaga tatctgttgc atgatcagct gattcatagt
    18481 aaagatcgcc tttttcatct gatgaaacca tttgtcgcat acgagactct acttctgctt
    18541 ttgagagacc agctgccgga tcgctttgaa gttggatgcc aagaccatgt atttcgattc
    18601 ctcgttcttt gagtgccatc gcttctccga tggttgcgat aaacgtactg tcgattcttc
    18661 tggatagatt gttttggtca ggtgcatcat agattcttga gattagagaa gtatttcccg
    18721 gccgatcttg cgtattagaa aactgagttc cgtaataatt gctgcttgat tgtgcgtgta
    18781 cccgctgtac tttataggaa aatgttggta caccatccgt cagcaaaacg atcacttttt
    18841 tatgtccatt tggaacggat agcatgcttc ctgcatctct tagtgctttt tgtgtaaaag
    18901 taccaccatt tgtccgtgaa ggtgtaatgg attttacttg atttttcact gaatcaaatg
    18961 aacccatctg tactgcaccg ttactgtagc tatatccttc gcttgagtag ccgacatatc
    19021 ccatattgat tttgtctgtg ataccgctat ctgctaaagt atcgacaaaa cgatcgacac
    19081 caatctttac ttcaccgatc cgattattgt cgttcatact tcctgaccag tctacgacca
    19141 atacgagatc aagaggcgtg atatcttttt gtacatttcc acgagcattc aaataaacat
    19201 caaacaatcc gggtgtactt gtttcctttg catactttcg tatcgcatag tctgcttgat
    19261 taccggttcc tccatattcg atatacgaat gggtccgatc attaggatct ccattccaag
    19321 aatttatacc atcccattga tttgttccgt ttttattgcc ttgatgatca agaacatttg
    19381 tatttcctgt aggttgccaa ctggctttag gataagtgcc ctgatcatct gttgtatatt
    19441 ctgtaggaat cgtttgatta gatggcacac tcacagctgg caaggataat gtaggcaatg
    19501 taaatgggct gacagaattt tcgttttctt ttgatgattc ttcttctatt tcgtctggct
    19561 ttggtactga cacatctgta tctttcttat tttctgtctc atcaataatg tcatattctt
    19621 tattatcgac gatcagcttg tttgcctggc tgtggtaaag tgctggatcg attggaaaac
    19681 tcagatcgat agtttgttta gtgtttgatg agatttccaa cgtaattttc ccgctattta
    19741 cctgataagc atagccatct ccttcttttc tttccacatc acttaagtaa ttttcgacta
    19801 gaccaatttc tttttctatc ttttcagcac tggtgttatt cgcatttagc ttgatgttca
    19861 gtgctttttt tccagactga tccgaaatga cttcagctga aattgtttct aaaactgttg
    19921 gatcatctgt agtgtctgct attgcagata atggcacaaa gtttgataaa ctcaatatta
    19981 tcagcatgaa aataactttc agtttcttcc ctgtggtcgt catttttccc ctcctttttc
    20041 tttttttctg ttttgacgtg aatagtgaaa attattaaaa taaacaggcg ttacataatt
    20101 ttatttattt taatatgata attattatac gaagggcgct aaagggaatc acaccaattt
    20161 tcataccaat tggtttcatt tatgaagttg attccttcga ttaatgagcg tttcattgaa
    20221 agaaaagggt tgagggagaa aaacatatga tcaggttgtt ggaattaaac tatagaaaga
    20281 tggaaaaaat cattcgaatt ctgtccattt ctgacaagtg gatgactaaa aaagaacttg
    20341 ccaaacgaat tggtagttca gaatctacat ttgtaaggta tatcgaagag atcaaaatcc
    20401 gctggggaac ggatgtaacg attcaatctt cacaaaaatt ggggtatcgt cttgaacatt
    20461 tgaatgtacc agcttattta aatgttctga tggaaatgtc gcaaagttct acaaacagtc
    20521 gtttattaaa tgagattatt gtcaatccgg gtaaaacgat tgagtattat tgtgaaaaaa
    20581 tttccatcag ccggtcttct tttacacgta agttgaaaca atgcaatcaa attttagaaa
    20641 gctatttatt aaaagtgatc gttgaccaag gttttcagtt aactagcctt aaaagtgagc
    20701 tccagttgcg tatctttgtt acctttttct ttttaagtta ctacggaaga cgcacgttgc
    20761 catttccaat cgaaaaaaag aagatcaaag aaattatggt caagaataat tgtcagatga
    20821 atttgtttca ggaagaaagc ccatatgagc aatctttctt tattatgtat tttgttgtgc
    20881 atctcatgag agaatcccaa ggaactccat ttgaatggaa agctggagac tcttatgtac
    20941 agttgataaa tatcgataaa aaagacttct taatcttgaa aaatctctta ggaaaattga
    21001 agtacagcag ctatataaaa gtagtgaact attttgtcaa tacgattttt tcagaatttt
    21061 cggagaaaga aaaaaatgaa atcagtgagc agatacgaaa aaatatgtcc tactgttcgt
    21121 tttcatccgc tgattgcaaa gaaaaaaacg aagaatatgt tgttcaatta ttaacgcaaa
    21181 tttatttttt ctcccgtatt ttcccatacg atacgactca tttgacgatc cgtgcaactt
    21241 actttgcaca gcagatgcaa caccgccaga tacgtctata tgctggattt cgtgcagaac
    21301 ttcaatttta ttccactcta ctgaatcaga atctgattta ttatttatct agctatatct
    21361 tttggatgct tatcggtaat cctgaaattc accattatac ttctgataaa aaaatactga
    21421 ttatcagcga cttaagtctt cgacatagtc aatatattga agaatatatc agtgatattc
    21481 ttgctgtgca taaaattcac tctgagacaa ctgctattac tgaagatcag ctgtcgaaat
    21541 acaatcttct cgagtatgat ctgatcgtta cgaatcaacc cattttgaat gcccatgttc
    21601 ctcatatatt gattgatgat agtgtgtcat tcgcaaatga agaagaactc atccgcttat
    21661 tcgagttatg aataatcaaa aaacagctat gtataaacca ccctgataaa aatttcagga
    21721 caaatacaca tagctgtttc tttttattgt attccttcag caatccaacg agatttatag
    21781 cgaaagtctt ttaacaactc ttcttttgtc caattatttg caatgagatc cgtcaaagtg
    21841 ttcattacga gggtcatgga aaagtatagg aaactaactg cttcttccga cgattgaatg
    21901 gagaaatgat tgattcttaa taattccagc catttttcgt tcattggaga atcagccgat
    21961 agttctctac gtttggttgt ctgctgatca tttcgctggg ttaacaagta taacctttgc
    22021 caatagatac tttcgtgatc taagtcgcag caccactcta aatatttttc gatccccaaa
    22081 aagaaatgtt gtttgttttc gctaatgaat tttaagatat cttgatggac tatgtgagaa
    22141 ctgcttttga tcgtatacaa ataagcatct tccaaatctt gaaaatattt ataaaaagca
    22201 ccgcgcgaca tccccatctt gtcaacgatt tctgagactt tgacttggct gatgtgctgt
    22261 gagcaaaata tctccagtaa taggtcatcg attctctgtt tctttgcgtt cgataaattg
    22321 aaaaaagttt cttttggcat ccacatctct cctttatctc tgattatacg tggatagccc
    22381 ttcattgtca tgaaggattc attccttctt tttctcttct tctgtcaagt acccatcttc
    22441 cattcgataa acgcgatcac tccattgaat catacggaca tcatgagtaa ccataatagt
    22501 cgctttttgt ttctcatgag cttctttaac tagtaatttg acgacttcat atgcatgatc
    22561 tgtatccaaa ctagctgttg gttcgtctgc taagatcaag cttggctcat tatataaagc
    22621 gcgagcaatt gccactctct gtctttctcc accagaaagg tctttaggat aactattttt
    22681 aagtgactgg atatctaaag attgcagcaa ggaatcgatt tttgttgaat cttctttttt
    22741 tgaagtaact tggtcaacta gttcaaattg ttcctttacc gttaaaaaag gaatcaaatt
    22801 agagctttgg agaataaatc ctatttcttt aaagcgtaaa tctgctcgtt ttttttctgg
    22861 aagctcagtt agtgagtgac cgttgatcac tatttctccg gaagtcggtg tttgcaatcc
    22921 tcctgaaatg gtaaggaaag tacttttacc tgaaccagaa ggaccaatga tgctgacgaa
    22981 ttctccttcc tttacataaa aatcaattgc tttcaaagct gagacctttg tatgaccaga
    23041 accaaatgtt ttggtaactt ttttcatcgt taatattttt ttcattttta ttaacctcct
    23101 attgctgtaa ttgggtctac tttggttatc gtacgtatcg aaaacaatcc gcctagcatt
    23161 gcgacaatga tcaaaatccc actatatagc aaccattgtg accaaaaaac agcaaaaggc
    23221 atcgcgcttg gtaaaatcag actagtcaaa taagcaaaca aaagagcaag cagaacgcct
    23281 accaccccaa caataaagga ctgtgctact aaagatttag cgatgaagaa atttttgatc
    23341 ccttgtgctt tcattactcc aaaaatagca gtcttctgaa gtgtcatcac gtacatgaaa
    23401 ataccgacca cggcagcaac cactaagaac aaaaagtata tcatcgcatt taaggtcata
    23461 ttttgggcag aatagccagg aatactttca atgaatgtcc caatagttaa taactgtaga
    23521 cccttcgatg ctttttcttt cgaaatttta gctgaatcct ttattacgat cccattgatc
    23581 ggctgttctt cttcagaagc aaacggctgt tgtccatact tcaaagcagt ccacgtatct
    23641 agggacgcat agatgacggg agaaactgtg taataagttt caggaaagat accgacaatc
    23701 gtcaaaggat cgtcgtaact gccaattttg atcgtatctc caattttata gcctgaatca
    23761 gccagatttt gagagatgat gatttcattc tttgttttaa attcatgccc tttcgtgagt
    23821 tttggtaata aaaatgcttc tttcgttgtg ccgaaaactg taacattttc tttgtttttt
    23881 cctttgattg ctccgctgta caatccgatt ggtgctttct ctcctccttc aatgtatttc
    23941 agatctcctc ttgttaattg tgaagctgca aatacttgat ttgcatcctc cgacaaaaca
    24001 atctcttgtg cgtcccaatc atcgattgct tttttaaact cttctgaaag tccattcgct
    24061 agtcctgaca acatgaatac gacatacgca atcaagaaca taataccgac gatcaagcca
    24121 tatcgtaatt tagaatagcg aatttctttg atagctagaa acatttgttt ttcctcccta
    24181 tgatagattg atagtgacaa aatgtcactt gttgaatagt gtaactctaa agtgacaatt
    24241 tgtcacttgt gttgtttttt tcttatcaat aaattgtcta agacatcttt cgataaaacc
    24301 ttaacatgag tcataaaaaa tttatttaag ccttatttat gctttccatg ataaactaaa
    24361 gtgaccaaat caatgaggtg ggaaaatgat taaactatta gtagtagaag acgataaagt
    24421 attatccaat aatataaaag aaatactcgc tgcaatcggc gagataacac aagtttatga
    24481 tggtagtgaa ggactgttcg agttggaaag tggagtttat gatcttgcaa tcttggattt
    24541 gatgatgccg gaaatgaatg gatatgaagt gttagcaaaa atacggaaag aaaaaataca
    24601 gactccagta ttgattttga ctgcaaaaga tagtttggaa gataagattg aaggatttga
    24661 acgaggtgca gatgattatc taacaaaacc tttctatcga gaagaactga tcatgcgcgt
    24721 gaaagcatta ttgaaacgct cgctaggtct ctttgatgaa aatgttctga agtataagga
    24781 tatcagttgt gatttaaaaa gcaatgaagt cttgtataaa gaacaagttt tgcccataca
    24841 aggaaaagag tttgaattgc tcgtttattt tttacaaaat aagggaatca ttttgacaaa
    24901 agagcagata tttgaccgga tctggggatt tgattctgat acgacgatta cagttattga
    24961 agtttatatg agcaacttac gaaaacatct gcgaagtact ggattggata aagaaatcaa
    25021 aaccttacgt aatgtcggtt atatactgca aagggtgtga tgcaatgaat ggagatatta
    25081 cacgaaagca acgtagtcgc ttttttgcga caaatgtagc agcatttgct tttatctttt
    25141 ttcttttggg attcattact ttacagatat taaaccaatc tgcatacaga gaaacagatc
    25201 tgtcattaga gaaaataaat tttgattctc gaacagtaca attagagata caaagatatc
    25261 agcaaggcga tcccttttta aacccgactc aaacggaaca aaacagaaca aacatggaac
    25321 agccattaat ggaaccatca aatgaccgtt ttaacactca agtgatcttg tggtctaaaa
    25381 atggtgagat attgaataaa gcagctattg gtgggcgttt caaccaaatc aaagaattaa
    25441 aaatggatac gaaaaatctg aataccatcc aaaatattga attaaatgaa gacagtggct
    25501 catctgccct ctctttccgt tcgattacga aaaaagcgga gacgaatcaa gatggcgttg
    25561 cctacatcca aattttagaa aatacaaatc aaatcaagaa ttcattagcg acttttcgca
    25621 cgattatgat tttgtgtatg attgtttttt ggattctctc tattggtatc agttattact
    25681 tgtctagtct aagcatgcgc cctattattc tttcttggag aagacaaaaa gaatttgttg
    25741 agaatgcttc tcatgaattg cgcacaccgt taacgattat tcaaaatagt ttggaacatc
    25801 tttttaccaa gcctaatcat actattattg aagaatccga aagcatcgct caagcattgt
    25861 cagagacgag gcgcctcact ggattgactt cggatttgct gacaatcgct cggaatgatt
    25921 ctgatcagca gctgctttca aagcaaatga tcaatacaca agaatatatc gaacaacttg
    25981 ttaaaccatt tcaagagatg gccatcatag atggaaaaga gtttattctc cataacgatg
    26041 caacattaga agtaccaatt gatgaaaaaa aaatccatca agttttggtt attttgctgg
    26101 ataatgcatt aaaatatacg aatccagaag ataagatcat tttggattca tctgttaacc
    26161 ataaagaatg gctgattcac gtaaagaata ctggcccttc tatcagtgaa gaagatcaag
    26221 caaggatttt tgatcgattt tatcgggaag atcaatctcg ggcgaaagag actggcggat
    26281 acgggcttgg cttggcaatt gcaaaacagg taatcgaaca gcatcatgga cagatttctg
    26341 tatccaatta tcttccacaa ggagtcgatt ttaaagtcca attgccgttg aagcctaaaa
    26401 aataatgctt atccctaaaa aagggctgtg acaaaaatct tgtcacagcc cttttttgtt
    26461 gcgaataaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt tctttaagct
    26521 gaccacttct gtcacaacct ctttagctta cgcttacgat tttttattgc ggtcttcaat
    26581 gtgtacatca ccgcttgtgt caatatccaa ttcttctcgg ctaacatctt ctgtcacttt
    26641 ttcaacatct tcatgttctt ctttatgaat cccaacttct tcacgaataa ctgtatgttt
    26701 gcttacatct acttgctctt ctttgattgg gatagtaatc gtgtcatctt ccaatttttc
    26761 gtctgttccc tgtgaagatt gatctgttac tggtttgcgt tcgatgacga tttcttcacg
    26821 tttgaccggt acttctaccg tttcagtatc gttgaccaca tgtttgtgga tgtcgacttc
    26881 tccagttgtc acatcatgtg tatttacatc taatttttct tcttttagtt gaattgtatc
    26941 atcatctaga tttggtttgc gatcgatgtc ttcagtcttg cttgtaccca ttgtatcagt
    27001 ctcgccaggt acgtccatcg gatcagttcc tggtgtcata ttcaatggat ccgttgtatt
    27061 ttgaaagcct gtcgtgccac ctacgtccat tggatctgtt ccagtgccag tttctaatcc
    27121 agtccccatt gtgcttgctg ggctaacagt atcttgtgta tccattgtat cttctgttgt
    27181 ttctggctga cgatagcctt ttactgtgat gacaaatttt ccatcagaaa tatctcctct
    27241 atattgatat aaaggatcat tttctggtgt agccgtctca gaatcatagg tatcaaaaga
    27301 aaaagtatct tttatttttt cccataaaga acggtcttct tcaccatcca tccgattttc
    27361 atctgtttca acatctatac cagacaatcc gtcaaatcct agtctagatg ctgcttcatc
    27421 agtagtataa ataacgatat catctcgttc atacccttcg ttacgcaacc gttcaattac
    27481 tgataatgct tcttccctcg tactatagct tccgatgacc gttaaatttt tctcttccat
    27541 gtaaattcct cctagcaaat a
//
LOCUS       DAZUMK010000028        26021 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000028 DAZUMK010000000
VERSION     DAZUMK010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.028"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1125
                     /locus_tag="ACG808_001145"
     CDS             <1..1125
                     /locus_tag="ACG808_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS5046788.1"
                     /translation="SSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTE
                     GQTKTFSTPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDL
                     YASVMIAQAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQS
                     KFRVYENYEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQY
                     YLKLNELIKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAA
                     TQGEPIHAVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQI
                     IGYVGSTGNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1474..2106
                     /locus_tag="ACG808_001146"
     CDS             1474..2106
                     /locus_tag="ACG808_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFS5046789.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            2694..4133
                     /locus_tag="ACG808_001147"
     CDS             2694..4133
                     /locus_tag="ACG808_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5046790.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4428..4646
                     /locus_tag="ACG808_001148"
                     /pseudo
     CDS             4428..4646
                     /locus_tag="ACG808_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            4895..5110
                     /locus_tag="ACG808_001149"
     CDS             4895..5110
                     /locus_tag="ACG808_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046791.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5327..5583)
                     /locus_tag="ACG808_001150"
                     /pseudo
     CDS             complement(5327..5583)
                     /locus_tag="ACG808_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5770..6681)
                     /locus_tag="ACG808_001151"
     CDS             complement(5770..6681)
                     /locus_tag="ACG808_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS5046792.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(6700..7704)
                     /locus_tag="ACG808_001152"
     CDS             complement(6700..7704)
                     /locus_tag="ACG808_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFS5046793.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(7701..9824)
                     /locus_tag="ACG808_001153"
     CDS             complement(7701..9824)
                     /locus_tag="ACG808_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFS5046794.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(9829..12276)
                     /locus_tag="ACG808_001154"
     CDS             complement(9829..12276)
                     /locus_tag="ACG808_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5046795.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12263..12652)
                     /locus_tag="ACG808_001155"
     CDS             complement(12263..12652)
                     /locus_tag="ACG808_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS5046796.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(12712..13215)
                     /locus_tag="ACG808_001156"
     CDS             complement(12712..13215)
                     /locus_tag="ACG808_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFS5046797.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13228..13452)
                     /locus_tag="ACG808_001157"
     CDS             complement(13228..13452)
                     /locus_tag="ACG808_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046798.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(13556..15466)
                     /gene="ltrA"
                     /locus_tag="ACG808_001158"
     CDS             complement(13556..15466)
                     /gene="ltrA"
                     /locus_tag="ACG808_001158"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFS5046799.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(16212..16349)
                     /locus_tag="ACG808_001159"
     CDS             complement(16212..16349)
                     /locus_tag="ACG808_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFS5046800.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(16346..17530)
                     /gene="mobT"
                     /locus_tag="ACG808_001160"
     CDS             complement(16346..17530)
                     /gene="mobT"
                     /locus_tag="ACG808_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFS5046801.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(17789..18079)
                     /locus_tag="ACG808_001161"
     CDS             complement(17789..18079)
                     /locus_tag="ACG808_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046802.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(18069..19211)
                     /gene="dcm"
                     /locus_tag="ACG808_001162"
                     /pseudo
     CDS             complement(18069..19211)
                     /gene="dcm"
                     /locus_tag="ACG808_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(19287..19451)
                     /locus_tag="ACG808_001163"
     CDS             complement(19287..19451)
                     /locus_tag="ACG808_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046803.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            complement(19444..20118)
                     /locus_tag="ACG808_001164"
     CDS             complement(19444..20118)
                     /locus_tag="ACG808_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFS5046804.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            complement(20196..21536)
                     /locus_tag="ACG808_001165"
     CDS             complement(20196..21536)
                     /locus_tag="ACG808_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFS5046805.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            complement(21619..21954)
                     /locus_tag="ACG808_001166"
     CDS             complement(21619..21954)
                     /locus_tag="ACG808_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFS5046806.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            complement(21964..22338)
                     /locus_tag="ACG808_001167"
     CDS             complement(21964..22338)
                     /locus_tag="ACG808_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS5046807.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            complement(22351..22665)
                     /locus_tag="ACG808_001168"
     CDS             complement(22351..22665)
                     /locus_tag="ACG808_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS5046808.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(22778..26005)
                     /gene="fss3"
                     /locus_tag="ACG808_001169"
     CDS             complement(22778..26005)
                     /gene="fss3"
                     /locus_tag="ACG808_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFS5046809.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYCQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
BASE COUNT         7481 a         5444 c         4552 g         8544 t
ORIGIN      
        1 tcaagtgaaa gtagtacgat ttcaagtacc gaatcttcaa caacttcaag tagtacaagt
       61 gaaataactt cttcgaaagt gacaaaacca aaccatatag aatcttcaaa aaaggaagcg
      121 ccaaaaacgg aaggacaaac aaaaactttt tcgacaccac agcaaataga atcaacagaa
      181 tctttccaat cacaaccaat actatctggt aatgaagaaa ttcattttga caaaaatgaa
      241 tctgtagaaa attttattat gaaaattggt gagtctgcga gaaaaattgg tcagaaatat
      301 gatttatatg cttctgttat gattgcacaa gcgatcttag aatctgccag tggtcaaagc
      361 cagttagctc aggcaccgaa ttacaattta tttgggataa aaggaactta taatggaaac
      421 tttgtcatta tggtaacaaa cgaagatttg ggtaatggaa cgctttatac gacccaatca
      481 aaatttagag tgtatgaaaa ctatgaggaa agctttgaag attatgccaa attattaact
      541 aagggaatta gtggaaataa agatttttat gcaggagcat taaaagctaa tagtaaaaca
      601 tatcgagaag caactaaatt ccttacagga cggtatgcaa cagatacaca atactatttg
      661 aagctaaatg aattaattaa aacctacgat ttaaccaact atgataaaga agttaaaggt
      721 ccacaaatga gtaagactgg ataccaagtg cctattcata attattcaat aaccagtttg
      781 tttggaaatc gtggaaatga atttcaccga ggacttgata tggcagctac acagggtgag
      841 ccgattcatg cagttaaagc tggtaaggtt gttaaagcag agtttcacta ctcttgggga
      901 aattacgtgg tgatacaaca cgaagatgga tcaaccgcgt tatatgctca ccagcaacaa
      961 tacctagtaa aaactggaga taatgttttg caaggacaaa taattggcta tgtaggaagt
     1021 actgggaata gtactggccc acaccttcat ttggaaattt gcaaagatag tagtctttct
     1081 cagagcatgt tagttgatcc gaaaacctta ttattcggag actaaaaaag gtaatcaatc
     1141 tgacaaagta acaagaaaat ggttggattc ttagaccttt gatcaggata ttgagactgg
     1201 aattgatatc tcgattacga tgggtttgac aaataaaaca agtccattat cgaatatcga
     1261 gtgtattatt gccgtcttta tgctcatatt ttgagcaggt ttcactagat gaaaacaatt
     1321 tatctatctt gaggatttcg tgtacgtatc gttcagtctt gtattgtaaa ttagtcataa
     1381 aatcagacta agaaaaattg gaaatatttt tttctgattt attaaaagtt aattgaaatt
     1441 atatggtact tctttcttgg gagggtaggt gcagtgaagg aaagatatgg tcaccttact
     1501 gttgttgagg agattactca taatggatat aaaaaatatc gttgcctatg tgactgtggg
     1561 aatgaaacga ttgtatatcg atcaaactta ctatcaggta gaaccaaaag ttgtggatgt
     1621 ggaaattata aaaataggca taaatataaa aatttgacaa ataaaacttt tggtaagtta
     1681 gttgcaatca agccaactaa actgcgaagt gaaggaactg ttgtttggga atgtcaatgt
     1741 ctgtgtggga gaaaaaagtt agtatcggca aagcgacttc taaaaggtga tgtaaaaagc
     1801 tgtggatgtt tgaaatcaaa aaagcatcac attgaaaata aaaaattcgg attacttact
     1861 gcaaagtatc ctttggacaa taactgttca tggaaaacaa aatgggtttg tcattgtgag
     1921 tgtggcagta tgtgtgagat aagttatagt aacttatttt ttggacatac tcaaagttgt
     1981 ggatgtttaa aaaggcaaga aacaaggaca tgtgttgaag ggacagtaat agagtgtcta
     2041 acgtcaaaat taccgaaaaa taatacaagt ggtgttaagg gagtttatcg ttctaaagag
     2101 aaatagattg cgtatataac tttaaaaaaa aagaggtact atttaggatc gtatgattct
     2161 attaagagtg cagctcaagc tagaaaagat gctgaaaata aattgtttca acctttgatt
     2221 gacaaatatc gaggtaatca ttaattattg tggataataa ttaacagtga tatataaaca
     2281 tctataatat attattcata ctattgatat ttagtataat cctatggaat tgaatatcat
     2341 agcaatgtat ttgaaaagca aattggaaaa caaataggtg caacaccata aatatgaaaa
     2401 aattgggaaa aagtagaatg gaaatttgct tacgtctcac gagatagctc acactattaa
     2461 tctgaagaac aatgaagtcg acttttagaa attgctagaa gaaagtaaag cgaattatca
     2521 ctagttacta tagagtaagt tcaaataaag catctccaac ttacatggtt acatgaggat
     2581 atcaaatcga aattactcaa gcttcctatg gctagagtcg cgagaagttg ttgggcagta
     2641 gacataggtt atattgttct tagctacacg atcattagaa aggaaatcct attatgtata
     2701 gttatcaaga agatgcaaaa gtccaaaaaa atcgctggtg gattttgatt gctatatcta
     2761 tgtttacctt catgtccaca ttggatggca gtattgtcaa tattgcacta cctaccatct
     2821 cagatgatat gggcatacca atgaatcaag cggaatgggt agtgtccgtt tatctgatga
     2881 ttgtctgttc ttgtttgttg ctgtttggta aaattggcga tagccgtggg aaaatcaaag
     2941 tatttcggat tggtacgctg atctttgtct tgggatcgct gttgtgtggg ttcaatcatt
     3001 cgctaggtat tttattgttc gcacgggtgg tacaagccat tggtgctagt atgactatgg
     3061 ctacaaatgc cggaatcatc acggaagtct ttcccataca tgaacgtggt cgggcattgg
     3121 gagcaatagg tgcgtttgtt tcattaggct cgattgctgg accaggtctt ggcggcttga
     3181 ttttgtccca attttcttgg gcgtacattt tttggatcaa tgtaccggtg ggaatcgtaa
     3241 cgatgttgat tgctgaaaaa ttcttaccaa aagatatcac catgagcggc catacaatcg
     3301 atggaggggg ctttgctgcc attgcggcta cgattatcag cttttttgga gcaatctttt
     3361 tggggcaaga aagtggcttt ttccatccag tcccccttgt actgttcgta atttcagtga
     3421 ttgcctttgt cagcttttta aaagttgaaa agaaagcagc ctctccattg atttctttcg
     3481 gtatttttaa gaacaagatt tttaccatga gcttgattac agccttgttg attttttctt
     3541 ccaacttctt tgtcaatgta gtgattcctt tttatctgca aaatgctcgc gggttgagtc
     3601 ctagctatgc tgggctgttg atgatggtct ttcctttgat gatggtcgtc ggggcgcctt
     3661 taagcggcta cctgaccgac cgggttggcc cggagatttt ggtattagta ggcttgggct
     3721 tattgtcggt aacccaggtc atgtatatgt ttttgtcagt ggactctcct ttgtggctat
     3781 acattgtcgc tactgccatc atgggtgccg gcaatgcctt gttccagtcc cccaataata
     3841 cgatggttat gagctcggtc actatcgaaa acttgggact ggcaggctcg atgaattcct
     3901 ttgcccgaaa tttggggatg gtcagcggga tttcgctttc tacgacggtc ttatatcaag
     3961 cgatgtctgc taagatgggc cagcaagtaa ctaactatat cgcaaatcgg ccggatgtct
     4021 ttttatatgg aatgaagatt acctttttag gcagctttgt tttgtgtttt gttgcctttt
     4081 tcctgactat ctggcgtatc aaaaaacaag gaggtattca gaaaaatgtc tgaaaaaacc
     4141 actgttgaag aatttttgga ggcctgcgta tgggtgagtg ggatttccat ggatgatgga
     4201 agatgaagtg aacttctaaa agccgaaaaa ctcaagtatt atcttcaaaa tactagtgct
     4261 caatgagatg cttgatttga tcacaagaag actgcgaaag ccgctggcaa ccgcaagcta
     4321 ggtatcccac cgatggacta cgttttagag gctactggtt atgaatacgg agccaacgct
     4381 ctcatctgca taagaataca tcacccagat tatgtaaaga ttttcgatac ttctgtcaaa
     4441 aaatcattaa gatagataaa tgatttccgt ctagtcaaat tggttcagaa tgtagacaca
     4501 cagatggtaa gaagtcactc acaattcgag aatggacttg tcctatttgc cgtatacatc
     4561 ataatcgaaa cgtcaacgat agtatcaata ttttgaccaa gggctatgaa tacaagcatt
     4621 gccttagaaa acatcaagaa ccttaggaac aacggggata gcttggttaa taagagatac
     4681 cgctgtttag gcttgcaagt atactctttt atcaagaatt ccgtggcttt agtcatgaga
     4741 ggcttaatac tatgaaaatt ttctttagca tttttgattt aggttccttt tctaattata
     4801 ataagatgac cactacagta acctactgaa atatataaac agccactgta gtaccatttt
     4861 caaagtagtc tatgagagga gaatcttcat tcttatgaac gctggaattg cggatatgaa
     4921 tttaataaaa aaaaccctta atgattttac ttccaattca atatcaaaag gtactggtat
     4981 caatttaagc acaataaaaa agctgaaaag tggtgaaaga tcagtggaaa agctgaatct
     5041 tttagatgct ataaaaataa cggaatttgc tatgaaaaat ggaaaagctg agattgaaat
     5101 atggagataa ttatcgtcta gtgaattact tgccacttag ctgaatttaa cgatccttgg
     5161 tgcttgaagg gggagattct tgggaataga gcatacttgc aagcctatgt ctttactaac
     5221 aacgatatct cttattaacc aagctatccc cgtagttcta aggcttttac ttttatctaa
     5281 gcaataatcg tattcaaagg aaatttttga cccttagaaa gtctcatttt atcagacgtg
     5341 agcttaggca ttgctgaatt ttgttaattc ggttttaggt tgtgctttga gcataatatg
     5401 aaagtattct ttatcacgat ttcattcatc taaggtaatg tgatgtgatt ctgaaattct
     5461 ttcaaaggca gtttttgcat aagctgacat tacatcatca atgacatgtt tacaatgttt
     5521 cactactaaa ataagggggt aaccaagaag aaaaactgaa tgattatttg tatctaattc
     5581 catggtatat caaccatttc ctatatagac tgattatgac atggattaaa ataaaaaggc
     5641 actatgtctt aattgtcggt tttcgagtta ccgaatggca ttcatcaccc acctatagag
     5701 gatgggcgac ttctgcctga aataagttac actacttata acttctaata aaattagatt
     5761 gagtcattat tatttaacat caacgatttt ccagtttcca tttttttgta atactaattt
     5821 gtactgaaag tagctagtgg tttttgatac attatcaagg tattttatct ttgtactcac
     5881 tagaaaattt tttccgtttt gtagataaac tggattctct aactccgaaa actttaagtc
     5941 aatattgatt ggtcgcatcg caccattttc aacataatac tccaattctt tttcagaagc
     6001 tgatggataa agtttaaaaa atgtttctaa aaaatcggtg atttcttccg ttgttttggc
     6061 atcaatttct gaatcgtttt ctacttgttt tgcttcatat tttgccttgt ctggctttcc
     6121 aatcattgtt ggcaaactag ttactaaatg attcctgttt tcatcttcaa agattgtcac
     6181 tcgatatgca gaagaaattg attgcgtctt ttttccctct gtaatatgtt gctccactaa
     6241 aaatgaaacg acaaattctt ccgagtgttt ttcaacatct agaattttta cagattgaac
     6301 ttcggaactc gtgggaatat ctgctcgaac catatcttgg attaaagcga gaccgttttc
     6361 tgtaaggtac tgttcgagat tactcattct cttttcaatt gcttctttgt tatttttcca
     6421 tgaaaaatat tctttcgcaa aatcctctgt aaaactttca acgccagaag tattcactac
     6481 cttctctttt ataaccactt tttcatgagt tgtatgttga tcaatggcgg tgaaattttt
     6541 ataaacgcca aaagcaagac tacaagacaa taaaagccaa agggcaagca ccatttttcg
     6601 atgcttaccc acggatacaa ctcgttcttt tttctttttt gggctctttt ccttttgatt
     6661 ccgttcaatt tttatcttca ttctttttcc tcttttctct tatttgtatc ggccagctcc
     6721 gataagatgc tgccgccaat agttttccgt tagattcgtc caccccactg gatctcctgc
     6781 atgaaacatg cgattctccc ctgcgtaaat gcccacatgg gtcacataag taccggcatc
     6841 ataggttgaa tgaaagaaaa ctaaatcacc tggtttggca tcttttagat caatatgagt
     6901 catggcatca tattgatcct gagccacccg aggaagtttc aggtcaactt tggcataaca
     6961 ccattgggtc aagccactac aatcaaaccc cgtttctgga cttgagccac caaacacata
     7021 ggccgttcct tcatacttaa atgcctcatc aaaaatttct tggactattt tgtcatcaaa
     7081 ctgagttgta gttaggtatt gcttcactaa taaacagtaa aacatattgc cataattata
     7141 ccgccaccca ccattttcct taatcgcgat tgggttgggg taatcgactt tttgaccacc
     7201 tgatttttcc tttgaaaaag attcagctaa agcaaaacta tgcttcttcc cgtttttccc
     7261 gacatgatct agatacccgc caccatagtt ataggcctga ataatactat cttgatcaca
     7321 ccctagttct ttggaacgct taaccaaaga cgcaaaatag agacagcctt gtttgatgga
     7381 tgcctcggta cttaatgaat taggtggtaa tccggctgac tcagaacttt gcataacgtc
     7441 ttccattgtg ccaccagatt ccacttgaat aatcgccaaa agaattggaa tatactctgt
     7501 tatatccgat tctttggcat acttttccac cattgcacgg tgtttcaata cttcttctga
     7561 gacactagtt ccgccaggag aagtgccaga actaccattt ccctcactgt cttcaccaaa
     7621 caacaaaccc acacacatca aaatacccat aaaagagatg gtcagaaaag ttaatgaaat
     7681 aacacccact ttcttcaagt tcattttttc tcacctgacc ttgattttgg acgatggttc
     7741 attctttttt gtactttttg attggtctgc tttggcagag tggacctctg tttcacttgt
     7801 tggaaagaat ttttcttact tgctgattgg cttttccctt ttgaaatgat ttcttgacta
     7861 gttagttttc gtttgatttc gggattcaga tttgctctca caatccgctt tggtgcttct
     7921 tttttcagca tcatatcttt ctgaggttga gctgctttat ctattggaat attcgccctt
     7981 ctttttccct gacgatttgt tgcttcactc agtatcttgc gtttttcgtc cattttcgca
     8041 tttcttcgtt gtcgttgttt ttctcgcaaa tccactcgat ccgctttttc ttggaacagt
     8101 ccgcgcttga attcttctgg tgcttttttc gacttttcga ttcctttatg gagattgtat
     8161 ttcaaattcg ttggcgtatt ccttacctga tcctttactt gtttagtctt atctttcacc
     8221 aaagcttggg tatccaaaac tttgccgatt tttctcccag ccaaattcat tcgggattgt
     8281 ttcttattat ttgaaactgg attctcctta gttaagtcag atagtatttc gtgatcgttc
     8341 ggtaatcgtg aattctttcg tagagaagaa ccggatggct taaatttgga ttttgattgt
     8401 tcctttgcca acttatttcc gactgctgca ccaccaccaa aagccaaagc tcgtcccaca
     8461 tttcgattga gttgccgcaa ttttcgattc attaacattt gcggttttcg catgacgcgt
     8521 cttcccattg actggctatc attactctgg aggttgaaca tactcataat gtcgcccaat
     8581 ttgaaataga taccggcaaa ggtcacaatt tgcagaaaag caaccatgaa aaatggatag
     8641 gttgccgaaa tattgaaaaa catcgtggag atgctaaatg cagtcgtaat caccaaagta
     8701 acacccgctc gcatcataat cgtattgaat aaacgaataa tcgccttctt tcctaaactt
     8761 tcataagtcg gcaacatgga caataagaaa ctaattggca gaaacatcgc aaagataatg
     8821 aagagaattt gcgagaacaa cataatccct gttagaagaa aaacaaagaa ggaaatgcca
     8881 atattgaaca ataccaaaaa gaccacggtc cctaaacggt tcatggtctt ggtaatactg
     8941 agattcgcat tgtcattgtc ttcaatctct gccttgaccg cttcttcccg atctttgcct
     9001 ttgttttcat ctggactcac agatagaatc ttgttgaccc ggtcttcacc aatttcttct
     9061 atgttggaat catcaaattg cagtagtaac caaggttgtt ggacttgaat cgaaaacaaa
     9121 ctatcccgaa tcaaatccac actatctttt ccttggcttt ctgaattggg tacaacgatc
     9181 tcggttccaa gggtaagggc cgcttcacta acatctgagc taaagtcatt gatttttgtg
     9241 atataggtag gcgcataagc aatgaacgaa cccgatagta gaaaaatcat cacaaaatta
     9301 atcacggctc gaactgcttt cgtggtttct cgttttaata atccggtata cgccacatac
     9361 acccccagag ctaaaatcat caataataga aacccaaaat aaaatccgga ggtctggagt
     9421 ccattttcag taataccagc caatgtttgg atattctttc caatactttc agccgtatcc
     9481 gaaatgaaat ctaatttgta agcctcttga accacataac ccgtggcgtt cgacaaatac
     9541 aaactcaccg tccaaatgaa attagtaatg gcataaagac catacatcac gcttttccct
     9601 aagccatcac cccaattcca aggcaaccaa tcccaagagc tatccacaaa gaaatctagt
     9661 tgatagttgt tcagtgaata tttggaatat agatttcctg cttcaactgt atcatcaact
     9721 agccctgtcg cctcggctac cgtaccaact aaactcaata gaactatcag agtgagtatg
     9781 ctaataaaaa cgattcccac aatccgaaaa agtttctttt tcatcccctc actccattct
     9841 ctcttgaaca ggtggacggg tatcaaaggc atggaagagt tcttcaaaga ctggatgtac
     9901 ctgaaccacg cctacccgac catacaaatc ctgcatcaag cattggccat tttccaaatc
     9961 tcgtaggcgt ttttgattgc tttcatcttc tcgatccaag ccaaagaatt ccaaggtttt
    10021 cttgatttca tgaatatcgg tactgcgaaa cgcaaacttc aacccgatat tattcttcaa
    10081 gcgttcatcc agtaaatcat cactattttg cgtgacaaag taaaccccgg cattcatagc
    10141 ccgcccagca cgaacgagct tattcgataa tgttttccct tgcgctactt gaagaaaact
    10201 ccacgcctca tctaagtcta caattttgaa gatttctcga ttactatgga taaagtccaa
    10261 ggcaaaagtg gaaatgacga ttagcatgga aacactaagc aattccatgg tggtatattc
    10321 ttcaaaagtg gtttctgcat ctggtaaaac caaatccgct acttgaatga tatttaactg
    10381 cctatctaaa ctaatcgact gggtaatcga accatctgag aaaagcaaat gggcaaaatc
    10441 ataatcggta aaggactcga tgtgatccgc gatattttcc gcaactggtg agccttcttt
    10501 tcgaagctca ttaattactt gtaataatcc ccgttgattg ctttgggtaa ccgtccgaat
    10561 ggccttacgc aacactggaa acttctcccc atctcgacta gaaattcctg ttaaaaaggt
    10621 cagaatatca attgctagac tttcagaatc cttctttttc tccataatca cataaggatc
    10681 aagtaatcct tgattttttg gcttactagt taagttgaga atattgattt catccgcaat
    10741 ttctggcaag gtttctttcc agccaccccg ttcccctttt ggatcgacaa tcacagcttg
    10801 tccaccaaaa aggacggcgt aataaactaa gagattgtta ctaaaggact ttccgccacc
    10861 gagtgaaccg aggaatgccg ctgctagcgc attggtaact gaatccttga ccccttgtga
    10921 ggccaatgcc ggtttcaggt aaacattgcg ccccgtatct acgttataac caaagtaaat
    10981 gccttccaac tcccctaaca tctgagtggc gccaaaacca agtccggcga gaaaatcaga
    11041 cgtaacatac tggatataat cattcatgta tcgcttacta gaaggtaaga attcttcatg
    11101 tagccccatc atatcgccaa aaggccggac tagttttaca ttggtatcat cataaaaatc
    11161 tagcacttca tcacaccggc gctttagttc atctactgat tcggcactta ctcgaaccac
    11221 atagctcagt ttgtacatgg attctttgga ctgatccaag gtagtttcca attcatctac
    11281 ggaatccaat gcatctaaga cattggaatt tgtttcattg tcagattgat aggcatggtt
    11341 gtccaagtct tttaattcct ttttcttatt gcgaacggta gcaagtgcct ttttgttcgt
    11401 cacaatctcc acattcatac tagtgtcaat tgggaatgta aattgttgtt gctggtaata
    11461 gaaaagttct gaggagggaa actccatttc ccccacaatt gtattaattg ttaagtacgc
    11521 tacatatgat tcatgattct catgttccat gcataagtac cggggctttt cttcaatcaa
    11581 acaacgactg ggacgtaata agtcgtaacg tttcaccaaa gtttccgact tcagcttttt
    11641 ctttggtagc tgaaattcat attcttcaaa tggtgtaccc ttttcgccat agatatgttc
    11701 aatcaaatac atcagatcac tcggtgtgac tcgtctgact ttaaatcttc gagccaattt
    11761 actttccatt agtttttcaa gcttggtata tcggcgaatt tcttcattgg ataaggaaac
    11821 aaaatcgccc atcaaatgat gattcactcc atagacaaat tcttgaaagc cggagaagaa
    11881 agatttcttg agattcttta gattgacctc ttcatctgtc gcaatcagtt tgaagccaat
    11941 aaaaaagcga taatcaatct gactatcgcc aatcatggat actaatgcct ctgtctgtaa
    12001 atcaattcgt tgtttcgcta cttctgttaa tcgcccggta atttcctttt ttgatcgttc
    12061 ttgcgtggca cgaatactac tttctgtggc aatttgtaag gcatgaatct tgccttcacg
    12121 attttgcgca atcaattgac gaaagttatc gtggacttga tatttctgtt caggtgacaa
    12181 aaaggagtaa ttgtaaggta ttaactcata gtaggcaaaa cattctccct cttgattaaa
    12241 gacaagatta ttttcaatat acttaatcgg gtacatagtt cacactcctt accagagtaa
    12301 tgggttcgtc ccatcgtttc ttctgttctt tgactttctt tcctgcgaaa gtgaccttaa
    12361 atcgtaagtg atagctaata aaagatcgta aaaatcccaa tggtttctta ccgtcaaacg
    12421 ttttttgcga catgaaccaa gtaactgcca ccggtatacc taagtatttt aaaaaggccc
    12481 cctctatcat agatagtgga ggcacatggg caaatacgat gacaaacagc aaggataaaa
    12541 caaaccaagc catctggtta aaagtcacag gaaaaggcag ttgaaagtca ttaattgcat
    12601 aaatgacctt ttcaactgcc caaatactgg tgtagctttt tattttcttc atcaaatcat
    12661 tcctttaaaa agaaggatag tttagaaatc aactaccctt caataatttt gttaatatgg
    12721 atactcaaaa acacctcgac tcgttaccaa ataattgcct ccaatctcca agtctctacc
    12781 acacgcatca aaatcaatat acgtcttcaa gtgttctggt acttctccaa aatcaccagc
    12841 ttctacaatc aagaaatatg ctacatcact catatcatcg caatctggat aatagataat
    12901 atcatccaca tgctctacca tctcctcaaa agaattgaag aaagcatgtt gaatctcaga
    12961 agcaacttca ccaactggcg ttccttcaag ttcctccgct aagccacaca accgattaat
    13021 ttcctcaatt ggcgtatact catcaatctc aaagggaagt tcataatcat gaattgcata
    13081 ttcctcgtat tcgtcattta acccaattcg ttctttcact tcatcaaaat cgacaggtgg
    13141 ggtaaaccac gcccccacca attcaccttc gttatatttc ccgaggttcg caatataaac
    13201 acgcatctgt tccatcgtgc ttccccctta cgcaccgaca attcggttaa acaaactgag
    13261 taagacatcc ttcaccccag aagcattaaa gaccaatccg accgcaatca aagcaatcac
    13321 caaaaagcca atcagtttgg aaaactcccg cttaaatcct aaaaacaaac caatgaccac
    13381 aattgccagt aagactaagg attgggcatt ggttaaaaac cattgatata tggataggta
    13441 agttcctgat attataaata tctcaaactt ttccccccat tcacacggag cgtgcgactt
    13501 tcaccgcact ccgcgttcca tcgtgtttat attgaaaagt atttatgtag tagacctata
    13561 tcaagtttaa ttcttgattg ccagctaata ctctatattt atttagtctt atgagctgga
    13621 ctttatttag cttcaataga gctatatatt tttgtattgt ttcttctgtt ttagcgtgta
    13681 ttagacggtg taccgcctct tttaccaaaa taagattttt ataatcgtca tttccaccag
    13741 ctccagttgg tacaatatga tgacaatgta tttcattagc ttgttgaaat tcttccagag
    13801 ttatagcaca ttttccatgt tgagcagaaa atagcgaaat tcgattgtct gcgtattcta
    13861 ttgtgcgatt ttccaaagat aatttcatta actggtacat cagtaattta tcaacactta
    13921 gattggtgtg gataaatttt cttccctcta gagtgtagca attcgctaaa tacacctttg
    13981 ccattggttt tctgtgttgg atatatccaa cagggaaaat aggttcgccc tcgccagcaa
    14041 cataccgaag cattttagat ttaccatatc ttgttgcctc ctttgtcgta agttgtctgc
    14101 ctgtttttct caatctgccc cttttgtttg tacctaatct gttagtgaag atagccatta
    14161 cagctctatt cagtttcata caatccagat ttacattcgt tgcaatctga taataatttt
    14221 gcatacctaa taccattgaa ttataaagtc ttgtttcttc taattccgtt ttcccttttg
    14281 agggagaagc aattttcttt gcctgtgata ccagcttgtc tttttggttt ttgaattgtt
    14341 tatcacttat atgtgattta acaactaact tctttcttct cggtatcatt ttaatcttga
    14401 aaccaagaaa gtcggagtat ctttttctaa catttacaat tcttgttttt tcttgtgata
    14461 cttccaattt taatcgttgt tcaatccatt gagtgattgc tattttggct ttttcagcac
    14521 tttccttgta gcggcagaaa actctaaaat catctgcata acgaaccata tacatttcct
    14581 ttaactttgt tttcttcatt tctttgtaag cgttgctttt acatgggcta ccgcttttat
    14641 tgatatgaac cacatagttt tttgtaacag gattttcctg ccattggctt tctacccaat
    14701 ggtctaactc attaagaaca atgtttgcta gtagtgggga tattattccc ccttgcggag
    14761 ttcctttctc tggatatgtt attgtaccgt tagtcatttt gattggagct tttaatatcc
    14821 tttttaaaat gaatatcaag tgcttgtccc gtattcccat agcccatatc tgttttatca
    14881 gcttggaatg gttcacgttg tcaaagaaac ccttaatatc aaattcaatc acataatgta
    14941 gctggctgat ttgtaggcgg ttataggttg cttttattgc attttcaact gacctattag
    15001 gtctaaaacc atagctgttt tcactaaatt tagcttcgca gattggttct aatacttgtt
    15061 ttatacattg ttgtataagc ctgtcccaca tacaaggaat acctagaggt ctggttttac
    15121 catttggttt tggtatttct tttcgcctca ctggcttagg tcgataccca tgtggactgc
    15181 ctgcaacaat gtatcttact tttttgacaa ctgtttctgc tggcaatttg ccaatatctt
    15241 taattattgt accgtctgtt cctgctgttt tactgcctgc atttgctttg atatttcgat
    15301 atgccagtaa tatattttct ctcgatagga taatatccat aagatgagtg aaaatatctc
    15361 cattcttact tttggcatat aaatcatcaa aggtgttttg catttcgtaa tattcagcgt
    15421 gtcttaaatc ttctacacac aatgtttcat ttttattttt aggcataagg cttcactcct
    15481 ttacttgaaa ttgagtgagc ttttttatct tactcgtaat ccttattgaa acaacataat
    15541 acttttctaa tatatacacg acttgagggc attcctccat tctcattaca agaatttctt
    15601 aggttcgccc tctgcttttc agcattagtt caactgctta ttgttcttcg tcagtttatt
    15661 ctcttgatga gaacccaata cctttcctcg ttccgataat cttatctata catatatatc
    15721 cttaggtcac tagctataag cctgctggct tgataccgcc tgtaacgtta tatggcattt
    15781 cataacctat attcttactt tgccacacca gctacgattt ctcgtacctt tgcatttcta
    15841 caaagttcaa atttagaccc gtacattcgc tgtttcgtca gttccttata gaacattcta
    15901 accatagata ttttatagac ccccgacctg ttggatacgc tacccacctc tggatagttt
    15961 tcgatagaag atatatgttc atcaactatt acggtatctt tcagccgtct ttaccgagct
    16021 tcaaacattc tgagtttcct catacaatgc ttgtcggagg ctcagggcag gcgtttcaga
    16081 gcgttacttc atcattccac ctgtcagatt atcagttctc ctaataacca aactttttcg
    16141 ttcttttatt agtgccttag cttttcacta aggaacgagt cgcacataaa ttttgtccga
    16201 aattcatctt gtcactttcc tttcttttcc attgctcgat ccgctgctgc acttgcattc
    16261 atcaagcaca tcacagtaac accaagcata ctgccaccaa ataccaaaaa aatatctttt
    16321 acaattccca tgacgattcc tcttttcata taatcaaatt ttcaattgcc atactttgtt
    16381 gctccaaaat ctttttatgt cggtctgata agcgtgcatt ctgtaccata tcctggatat
    16441 aggttgtccg attcaatcga tccaactcct tagccatttt ccacgtaggg gcgacttgtc
    16501 ggcctagcca attcaaggtt cgttcaatgg tatacggttt tggctcggaa gttaattgaa
    16561 tctctttccg attccgtcca ataaaacgcc cccattgttc attcaagggc cagttggttc
    16621 gccttttacc ttctacttta tccgcaaatc gcaagtaacg attgataatg gaaaacgtgg
    16681 tgctctcaat gctacgacct tttaacaaat cttgaatcgc atgaaaagca cggtcatttt
    16741 tcagtcgaat ctcaaaacga tttttcgtct cagtttggtc taatggaatc cctaacttga
    16801 tatattgttc atagtctttt tcatacaaac agaaataaac ctcactcttg agactgccga
    16861 tatataaggt attgcccatg ccgtcctttt catcagcctt taaaagttcc ccggaacgat
    16921 aacttttaaa ggtacgaaac aaggagatac attcttcctt ctgacatttt ttcgttagct
    16981 caggtatatc caatagcccc actcgatcat tgattgccaa gtccaagcgt ttcatcacgc
    17041 caccggcctt tagacaatcc tcgaaaaagt cgtaccagct acgcttttga gcgagtaaaa
    17101 aagtttcaaa ttgtcggcaa cctcgcccct ttaattctaa aagcacacct ttatcttctt
    17161 catgagacaa catcaccaca atatcaccca tgacatactg ttcttgatag gagtaaaagg
    17221 cataatcttc atggagcata tactcaaatt tcaaatggag aatttcttga ataatttttc
    17281 gtgcatccgt tgtgggaaag cgaatcctta catagtcaaa cagtaacgtc aaaggcaaat
    17341 cgggattaaa gctttccagt tggtcaaaaa ttttcctcat ggtttttata gatgcggtta
    17401 cgccaccatt ttctatctga tttaagtacg gccgactaag tccacaagcc gtagccaatt
    17461 tattttgtga aacgccgtat tcgttgcgtc ggtcttttaa tagccgataa tctaaattcc
    17521 gttgtgccaa gttctcacct ccaaattcaa ggtgttaact ttttcaaatg agttaacacc
    17581 tatacaaatt acttactatc tttgatacaa caagcattct taatggttgc ttacttattt
    17641 tttgtgtttt tgtacccccc tgttagatac gggggtgaga catcatggtc ggctaacgcc
    17701 gtccatgaac aggcacagca ccacggagga tttctgcttc gcgaatcctc cgcggtcagc
    17761 gcctaacagt cagttgtgtt ggtttaggtt actcatctgt aataccattt acttccaact
    17821 ctgctttcat ttctaacaag tattgacgaa tatctggttc ttttgcttgg tccgcaatcc
    17881 gctcaaattc tgcaattact tgatttattt caattcgagc atccgtctcc tcattcatta
    17941 aatatggatc gccagcgact ttgacaccat tcttgcgaat atcaaatttt aaccgtcgca
    18001 attgttcttc taattctggg tctgactgaa tctttccaat cacccaataa atttctccaa
    18061 tcaactcatc ataaaccatg tacatccaac tcttttaatc ttctggcaat atcaaaaatg
    18121 accggaactg ttacagagtt tccggcttgc ttatacaact gactatctga attgacttga
    18181 gaatctcgat caaaagccca atcgggaaag ccctgaagtc gccaacactc tctaggtgtt
    18241 agcttccgaa tttgatactg atccgtgacg atcgcttgtt ggtctcctgt tagtaaggta
    18301 tgaacactct gttttcccag acgacctcgg cgacttgtgg aattaggaaa agccagattc
    18361 acactgtctt ctggcttcac ctcttgatat tctgtttcat tttttcgaat catgattttt
    18421 gtaccacctt gtttcgttga caaggtgggg gcggttccag taacttcata aactcgatta
    18481 ccttgttcaa actttcccgg taatagtcct gctacagaga ttccatccct tgctggcagt
    18541 gcgattttga ttccttcatc acctgttaag gttggcgcta cgccatctga accatagact
    18601 tgtccaccca ttcctcgatt ggaaggattg atatttacca aatttctgat gttttgatca
    18661 gatgttgaag acggtccaat gataggaaat acttgttcgg tacatgcgtc tcgagaatgt
    18721 ccgataagga atatccgctc ccggctttgg ggaatgtagg cctttgagtt aagcacctac
    18781 cactccacgt cataccccaa tccatccagc gttcggagga tcgtctcgaa cgtagcccct
    18841 ccttcgtgat tgagcaatcc tcggacgttc tcaaggaata aaagcttagg tcggagaaga
    18901 gtggtgaacc gagcgatttc aaagaataaa gtacctcgag tatcggaaaa tctttgccgc
    18961 tttcccgcaa ttgaaaaagc ttggcacgga aatccgccgc aaaggatgtc cactggtccg
    19021 agggattgaa taaactcatc tgatacactt gtgatgtcat gcatttccac ctccttactt
    19081 gtatcatgaa ttgctttata actacgtcta gcaaatttat caatttcaca aaaaccgata
    19141 caatgatggc tcgcctgttc catacctaaa cgaaagccac caataccggc gaataaatct
    19201 aaaaatctca tacattacct cattcattgc aaagaaaaaa gccaagaaca ttttgctctt
    19261 ggctcataca ttattaattt gtcctactat tttttattta atccaactaa tttccgttgt
    19321 ttttcattgg cctgatcggc tacatattta atcaccgttt tgaactcttt tgcagtagca
    19381 ttttccgata aatccgcaat gctcttattg tatttcttta caattttcgt tacttgttct
    19441 ttctcagtca tgtaattgaa cctccaaatc tttcctacta taccctagtg gtaaaaataa
    19501 ttctagatta tccgtagcat aatgaacttc ggaaatatta ttcgtataat cttttattat
    19561 gatatgaagc aatacatctg gatgtttact ctttactttt gcaatgttct ctcttgattt
    19621 gaaaaaagcg ttaataaatg cctttttcgg cacatgacgt ccctctgcaa tttcacgttc
    19681 ttgtgtaaat ttccaagcga ttttagggtc ttgataaaca tagaaaatcg tcactcgaaa
    19741 atgttttttc aacgcccgcg agatattttt ttcagcactt aatattgcaa aagttgcatc
    19801 cagaataaaa gaatagccat gatctaagac atatctaaat gcctgctcta ccaaccaaga
    19861 agatgctttt tgaaagagac tagagttttt tccattataa tcaggaaact gggtgcgaaa
    19921 gtaatccgca tcaataatca catggttatc ataattctca gctaatccaa tggcaacctc
    19981 tgattttcca gccccaggac tccctgccat aaaaactgcg tctcgtccaa cttcggggga
    20041 tttccctttg ataatataag aaataaactt atccttattt gcttttgcat actctaaata
    20101 agaatcatta ctttccatat taattatccc ctttcttcct actatataaa agtattatac
    20161 gttacttgaa gtataaagtc agtattgaag atcactcaat cattcatctt ttctaattta
    20221 ctaattgaat ctaagaaatc ataccctttt ggtacaagtg gcgtataaaa ctcactaatc
    20281 acactgccac ctgtatccac ataacctcga cctttgatgt gcttcataaa gaagtttttt
    20341 tcgacctccc caaacatcat ggaatatccg agttcactca tacgacccaa agccacgcga
    20401 aagttgaatt gatcgcggat tccgtctcct aaatatttcg catccggcct ttggcaagcc
    20461 aaaatcagaa agaaaccaga ttgacgaccc aacatcacga tttgtttgag cttattcaaa
    20521 atcaccgcac tttctttcgt cgttaacatc tccatataag ccacgtattc atcaaagatt
    20581 aaaaagttgg gtggaagtcc aagataggca taattctctc ctgttttgta gttagacatt
    20641 tctttcattg ccttgctacg agccatcata cgttcataga aatcttccac acaagcagaa
    20701 atttcttcct tttgtgaata aacatgaggc atcaccgtac ctaaatcagc taaatctgca
    20761 tttttgggat cgaggataaa caattctgca tctgacttca atagtgcttc gataatggtg
    20821 aggaggaaat aggtcttccc tccacccgtg ccaccagcga ttaacatatg gggtaaagaa
    20881 tcataagccc atacttgatt tttcatcaat cgcagagtcc cattttctgc cactacttcg
    20941 tctatcccaa ttcgattggc gatcatatca tacagtaaag tatactccac ataggagtct
    21001 ttgagttcct tttctactaa ctcacaatac agtccgcttt ctaacttctt ttccaatttt
    21061 aaaagctgat cttggtattt cccaagtgaa atttgaacgc gaatagacag taatccatct
    21121 ttcattcgat aacagatttt ggggaagtaa ctgatagtct ctctggttcg gctactattc
    21181 aaatctttga aaaagccttc actttgagtt tgtttcactt cataccaatg gttctctaga
    21241 atcatccgag ctagcttttg tcgatgccac aagcttcgcc aatgatcaag aaaaaagtga
    21301 atataaagca atatcatagc tcctgaaaaa gcgagacttc caagcagaca tactaaatct
    21361 tttatagacc aaacgatttc aatagtagaa agtgatagct tagaaatatc aaattgtcta
    21421 aactcttgaa aataaaagac gctcatccaa ccgatgaaca gagcgagtag ccagccaaga
    21481 acaaagtgat aaaccaagtg ctgatctcct gcgcgtatgc gatgtccttt gtacattttg
    21541 atggtttcca cctccaatcg aaaaagccca ataggatttc attaggcaat ttcaaaaagt
    21601 tatgctagtc atttactgct acgaggaggg gagttgtttc gtttcaacca attgatttaa
    21661 aaagccttca atagcatccg gcatctccac accaatttct tccaaaatgg attctgcctg
    21721 ccgacataag ttttcattta cttcgacaac tataaagaaa gactcttctt ctcctaacca
    21781 gtctagtaca tcagagtctt cttcactgtc gctattttct ccaagtaaac cttgatgtat
    21841 aatcagaaac attttttcat cagaggttaa tgctttaacg agttgttcct cagtcagctc
    21901 gccttttcca tattgatcga gacaatctaa aatatgttca acattaattt ccataatctt
    21961 cacctatttc tttctttcat tttggttcgt aggatttcct tgattcggct gccgcttcaa
    22021 gactaaatca tccgccttaa tgaaccaatt aacggtagtt ccttctcgaa aaacataatt
    22081 ggcaacggta tccacgattg gattgaccaa ctctacctct gcatcataag caaactcgcg
    22141 taagggtaca ttagctggga tactaacttg aatcattcgc ccttgtgcag aggattttaa
    22201 attataggtt cgttctttta cctcattacc aattgttcca tcctcatttt gcttccgtac
    22261 ttctcggcgt aatgcggaaa aacgcaactt cccaaatgtt aaacctgaat ctacgacaat
    22321 accttctgtt aatctcatct tcttttcctc ctatgctttc actaaatcat caacatacaa
    22381 cacgtaatca gtaaacccac gattctcaat ctcatagcct ttggcaacca aatggggatt
    22441 cacggctttt agcggttggt cttgatcaaa atccttttca ccggcttccg cagctactac
    22501 cacctcaata tcatcggccc gttgcttact ggaatacaaa tgataactgc gaccaatcac
    22561 cgtagtattc cgtccatatc cttctgttat aatttctcct tcaccggcat aacttaactt
    22621 tccaaaggtt tcagccatat ctggcacgac aaatttcaat tccatcttct tttcctcttt
    22681 tcattgttag ttttggatac aaaaatagcg cgactacttt tttaggaggc ggtcgcgcac
    22741 gttggttctt aggatttact tgtatcatct catcacctta gttccggcga ttccagaaat
    22801 aatagcccgc cgtcgcaaaa atcaaagtaa aaccaatgaa cagtaaaaca ttggaatttt
    22861 tctccccggt ttgagggaag gtcttcactg tgggactaga ttcttgagag ccacttggaa
    22921 cagttggtgt ttccggttgt ttcggcgtac ttagtatggt tggaacttct tttggtgtta
    22981 aggtttgaga tttttctttc aaatcttcat tgtgttttcc attgatattc ccatctttat
    23041 cgtaattgat ttccgcaaaa gtaaacttgg ttccttctgg atattttccg gtatctactt
    23101 ttttcgcaag cacagttttg gtgaattctt tatcatttac ttcataatca atcttgccag
    23161 atttccaaat ttctttgttt gtaccatccg gtaataaagc atacagaatt gtttcgaaag
    23221 cttcttttga gccatccagt acatcatgag taatcgacac atcatcaaac atatccacca
    23281 catcaccatg agtaaaggtc tgagaaccgt tttctaggtg ggcctttgtt tggatagaaa
    23341 catggtgttc cactgtacaa ctgacagttt gggcttggtt attcaagcta acatccttcg
    23401 ctactggctt atcgcctgct tcaaaagctt ccttattttc atacacatag ttgaataaga
    23461 caatagtttt atttaaaact tgttcggcgg ttaatttatg ctgaattttc caagttcctg
    23521 ttttctcttt gttcgccaac gttgcgcttg tttctgcaat caccactggt ttggcttttt
    23581 catctttttc ttgggcagct tttgtcgcat ccacatcaat ggcttgcgct accacatacc
    23641 agtcttcttt gatttcatgt agattgtaac tcaatttatc aactagctcg gtagaatcca
    23701 gagaagttaa ttctttgttt ccgtctttcc aagtcgcaag agaagttaaa ctatcttctt
    23761 cctcaggcaa atcatagagc attaaacggt tcaaactaac agaaaatgct ttgttcgtca
    23821 gcttctcgta aggaacggtc accgttttaa ttggttgttt ttggtcttgt tcggtaatgg
    23881 taaagacata ctcactcttc acaaagtctt ctttgttttc cttaaaagta gaacggattt
    23941 ctaatggcgt aatcttttga aaaccttctg gggcttctac ttcttcaagt aaataatccc
    24001 cataaggaag attttcaaac ttgccataac catcaaaccc taattgctcg ttataagctg
    24061 tggtcgcttc atcttcggca cctgtgattt cattggtccc ttccaatggc gacactttaa
    24121 aggtcagatc attgaagcca gtttcggtag tgcccgcagc tgatccagca aatttaaaga
    24181 aatcaaagcc aaagcgaatc acttgttctt ttgccgtaac atttcgggta atcacggcat
    24241 ttttttcgct atcatccacc tttttgatgg atacaggata ctttgtttca tcaaggctat
    24301 atccttcggg cgcttttgtt tcttggcaat aatactcgtt gatagctaaa tgtttaacgg
    24361 caacttggtt cttttcgtct aaagtcaaag tgaccgtttc atcagaacct ttcgttccct
    24421 tcgctaactc tggtttaaaa gcctcactcc atttaacagc ttttccatcc ttagcagtaa
    24481 acaaggtgta ttcagcaccc tcaaagactg ccttaccttg tgctttatcc ccagtatctt
    24541 tgtcttcttt cgtcaaagtg gtttcgccag tgacttcttg attttgccct tttacgttac
    24601 tggtaacagg ggctacggtt tgattggcat acttcaattc gacttttaca ggtttgaacg
    24661 tattcacgaa cccatgacta gctttagttt cggtcacata ataggttccc aattccaacg
    24721 cattagcaat ctcttttggt gtttccgcat gaccgttttc atccgtggtc atttcttgaa
    24781 cgatttcacc ggttggactg tctttgcgaa tggcaaacgt attaccagct aaggaataat
    24841 tgtcattcca aagatcactc cctgtttcaa ctccgctttt atccagaatg atttgccctt
    24901 tttcccgtgc gttttttgaa gtcacgtaaa tggtttcgcc tgctttaatg gtggtagtca
    24961 tgggggtggt atcaatcgta taaggcgccg gtactgattt ttcggtaatg gttacttttg
    25021 taccatgagg aatctcatcc aatgtagcaa tgccttcttt gtcagtagtc acgtcttttg
    25081 caggtaaagt ttttccaaag tctaaatgga aaaccgttcc tggtacaaca tcacccgatt
    25141 ctttatcgac ttttttgatc tttaaagaac cctccgtttc cacatcaatt ttgattgtgt
    25201 aggtattagg tttatcaatc gccccggcca ttaaagtctg ttggccagcc tttttatagg
    25261 cgacaggtgt tccggtacca gcagatttct taagcgtcag cacaccactt tcttttgaat
    25321 tggcatttgg ggtgattatc aactgattac cattcacacg ataatcaatg tttgcggcat
    25381 tttccacaac ctcatcgaac tctgacaaat tcagattatt cgtatccgtc acagtagtcg
    25441 attgccctaa tactgtttta gccgtactat tatggaaggt tggtttcttc tgataatcgt
    25501 caatggcttg atttattttt gcctcaatgg ctgcgatatt tacggcacta ccatctgatc
    25561 gttttattga gtgaagggta tacccattaa cttcttgcca gatcatcttc tgtgccacaa
    25621 tatgagtatc tgcatctgta cctacttttt tccataaaac ggaaactaat ttggctttat
    25681 cggacatatc tggtaacggg tttttctcat agccaggagt gaactcattg taaatcggga
    25741 ctcctggttc aatacagaaa atatcttgtt taaagccgtt gtacactgca taatgcggaa
    25801 cagtgacttc tgacattgga cgaccatcac taaaagttcc ttcaacagca tattgatgtg
    25861 ctaaatctag ttctacggtt accgtttgtg gatgggtaat ttcatcagca aaagtaatcg
    25921 taggagcaat tgctccaaca attgcttgaa cagccaaact caaaatagtt aatacacata
    25981 atttcttttt ttgtttttta tacatgaaat gttctccttt c
//
LOCUS       DAZUMK010000029        25932 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000029 DAZUMK010000000
VERSION     DAZUMK010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.029"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..1089
                     /locus_tag="ACG808_001170"
     CDS             85..1089
                     /locus_tag="ACG808_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5046810.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNIKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            1090..2034
                     /locus_tag="ACG808_001171"
     CDS             1090..2034
                     /locus_tag="ACG808_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5046811.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            2034..2996
                     /gene="opp4B"
                     /locus_tag="ACG808_001172"
     CDS             2034..2996
                     /gene="opp4B"
                     /locus_tag="ACG808_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFS5046812.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            3023..3937
                     /locus_tag="ACG808_001173"
     CDS             3023..3937
                     /locus_tag="ACG808_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5046813.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            3963..5744
                     /locus_tag="ACG808_001174"
     CDS             3963..5744
                     /locus_tag="ACG808_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS5046814.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            6092..6778
                     /gene="rnc"
                     /locus_tag="ACG808_001175"
     CDS             6092..6778
                     /gene="rnc"
                     /locus_tag="ACG808_001175"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFS5046815.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            6798..10379
                     /gene="smc"
                     /locus_tag="ACG808_001176"
     CDS             6798..10379
                     /gene="smc"
                     /locus_tag="ACG808_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFS5046816.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            10388..11197
                     /locus_tag="ACG808_001177"
     CDS             10388..11197
                     /locus_tag="ACG808_001177"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5046817.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            11208..12206
                     /gene="ftsY"
                     /locus_tag="ACG808_001178"
     CDS             11208..12206
                     /gene="ftsY"
                     /locus_tag="ACG808_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFS5046818.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            12393..14660
                     /gene="gshAB"
                     /locus_tag="ACG808_001179"
     CDS             12393..14660
                     /gene="gshAB"
                     /locus_tag="ACG808_001179"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFS5046819.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(14749..15201)
                     /locus_tag="ACG808_001180"
     CDS             complement(14749..15201)
                     /locus_tag="ACG808_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFS5046820.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            15351..16301
                     /locus_tag="ACG808_001181"
     CDS             15351..16301
                     /locus_tag="ACG808_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFS5046821.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            16294..17253
                     /locus_tag="ACG808_001182"
     CDS             16294..17253
                     /locus_tag="ACG808_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFS5046822.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            17250..18005
                     /locus_tag="ACG808_001183"
     CDS             17250..18005
                     /locus_tag="ACG808_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5046823.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            18028..18981
                     /locus_tag="ACG808_001184"
     CDS             18028..18981
                     /locus_tag="ACG808_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS5046824.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(19625..19921)
                     /locus_tag="ACG808_001185"
     CDS             complement(19625..19921)
                     /locus_tag="ACG808_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFS5046825.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            20276..20632
                     /locus_tag="ACG808_001186"
     CDS             20276..20632
                     /locus_tag="ACG808_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFS5046826.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            20642..21541
                     /locus_tag="ACG808_001187"
     CDS             20642..21541
                     /locus_tag="ACG808_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS5046827.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            22061..22789
                     /locus_tag="ACG808_001188"
     CDS             22061..22789
                     /locus_tag="ACG808_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFS5046828.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            22934..24961
                     /locus_tag="ACG808_001189"
     CDS             22934..24961
                     /locus_tag="ACG808_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFS5046829.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            25069..>25932
                     /locus_tag="ACG808_001190"
     CDS             25069..>25932
                     /locus_tag="ACG808_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFS5046830.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENEN"
BASE COUNT         8632 a         4539 c         5513 g         7248 t
ORIGIN      
        1 ctttgttata aaacgattta ttctgtgtaa tgtccttttt tattaagaaa aacttccatt
       61 taggaagcag agaggggtat tcgattgtcc gataaccaat tattagaagt tcagaaccta
      121 catacaggat tccgactcaa agatgactat tacgatgcag tagatgacgt ttcatttact
      181 ttagacaaaa acgagatttt agcaatcgtt ggggaatctg ggtgcgggaa aagtaccttg
      241 gctactacga ttatgggatt acttgatcct aataacataa aaattacagg tgagatttta
      301 tataacgatc tgaatttaat cgacctgaac gaaacgctct ataataaaat ccgcggaaat
      361 gatatcggaa tgattttcca agatccgttg tctgcattaa atcctctcat gagaatcgaa
      421 gaccagatca aagaaggact ttcctatcac acaaagatga atgcagaaca gcgtcaagct
      481 cgtgcactag aattgctaga acaagtaggg attcctaatc ctgcgagagt tggacgccaa
      541 tatccccatg aactatcagg cggtatgaga caacgtgtca tcatcgcaat cgcaatcgcc
      601 tgcaaacctc caatcgtcat tgcagatgaa ccgacgacag ccctagatgt aaccatccaa
      661 gcacagattc ttgatttact gaaagacctt caggaagaaa caaaaacagg gatcatcttg
      721 atcactcatg acttaggagt cgtagcagaa atggctgatc gagtggcagt gatgtatggc
      781 ggacagttcg ttgaagtagc aggtgtaaaa gaactgtttg atcatcctaa gcatccttat
      841 acacgctctt tactgaactc tataccacaa gaaggaaatc atgatgcaga actccatgtg
      901 atcgaaggtg tcgttccttc tctgaaaaac atgcctcgta aaggttgtcg gtttgcacca
      961 cgtattccat ggataccagc aagcgatcat gaagaagagc ctatccttca tgaagtcgca
     1021 ccgaaccact tggtacgttg ctcctgctat aagcatttcc actttagaga cgaaaaagga
     1081 gaggtgtaga tgacagaatt aatcacaatc aaagacctga aagttcatta ccctattcgc
     1141 agtggattct tcaatcgagt aacggatcat gtatatgctg tagatggtgt ggattttatt
     1201 atcgaaaaag gaaagacata tggtttagtg ggagagtctg gctctggaaa atctactacg
     1261 ggaaaagccg ttgtgggatt agaaaaggta acagccggac aaatcatgta tgaaggaaaa
     1321 gatgtaacga agcgaagcaa ccgcaaaaaa atgggttata acaaggatgt gcagatgatc
     1381 ttccaagatt caatgtccag tttgaatcct aaaaagcgag tgttggatat cattgctgaa
     1441 cccatccgaa attttgaacg gttaagcgat caagaagaaa agaaaaaggt aaaaggactg
     1501 ctagatatcg ttgggatgcc ggaagatgcg ctatataaat atcctcatga attttcaggc
     1561 ggacaacgtc aacgtctagg agtagcccgt gctgttgcta ccaatccaaa actgatcgta
     1621 gccgatgaac ctgtatctgc tctggatcta tctgttcaag cgcaagtatt gaatttcatg
     1681 aaacgaatcc agcaagaata cggcctcagc tatttattca tttcacatga tttaggtgta
     1741 gtaaaacaca tgtgcgacaa tatcgcaatt atgtacaaag gtcgatttgt agaaatcggt
     1801 acaagagaag acatttataa tgacccgcga catatctata cgaaacgtct gctttcagct
     1861 attccgcaaa tcgatgtaga aaatagggaa agccacaaag aaaatcgacg aagagtagaa
     1921 caagagtata tccaaaacca aaaagaatac tatgatgcga ctggtcgagt ctacgatttg
     1981 cgtacgatca caactacaca caaagtggcg ttgaaagatg gaggtgcaag ctaatgtgga
     2041 aaacgattct gcgacgtgtt ttattgatga tcccgcaagt tattatttta agtgttctga
     2101 tcttcttatt agcccaagcg atgccggggg atccatttac cggtttgatc aatccaaatc
     2161 aggatccggc agtgatcgaa cagatgcgac aagcagctgg cttgaatgac ccatggtatg
     2221 tccagtactt ccgttggatc ggaaatgctt tgcaaggcga ttttggtcaa agtttcttat
     2281 acaaacgctc tgttgctgac ttgatcggcg aacggatcat caacacttta tatctatcga
     2341 tcttgacagt tgtccttact tatttgattg ctgtgcctct ggggatgctt gctggccggt
     2401 atcaaaattc aatcttggat aagattgtcg taatctacaa cttctttagt tttgctgttc
     2461 ctttatttat cttcggcttg atcatgctat ttgtttttgg ttatcgttta ggctggttcc
     2521 caacaagtgg atcacaaaca ctaggtttgc aaggtggatt tatcactcaa tggatagacc
     2581 gtttgaaata tattctgtta ccatcaatta cccaagcatt tttggcgact gccgtcacaa
     2641 tccaatatct gcgaagtgaa gtaatcgatt cgaaatcgct cgattttgtc agaacagccc
     2701 gttccaaagg ggtaccgacg aataaagtat tcagtcgaca tattttccga aatgcagcgt
     2761 tgccgatggc ttctcaaatg gggtatgaaa tcacttcgtt gattgctggt tctgtagtta
     2821 tcgaaaagat ttttggttat ccgggagtcg gaaaactatt tattgattcc atcggccaac
     2881 gggactatac cgtgatcaca tccttagtat tgattttagg aatcgcgacg ctagtgggga
     2941 cattactttc agatatcatc atgagtatcg ttgacccgcg tattcggatt caataaaacc
     3001 aatcagaaag gagaatggat cgatgagtga aaaaaacaat acagcagttc aagaaagtat
     3061 tcctcctatg gggatacgaa tgattgctcg agaatttaaa aaagataaaa ttgctatttt
     3121 ttcattaggt ttgctagtgg ttttattatt agtgatcttt atcggtgcga tgctgaccga
     3181 tcaagacaaa gtgatgtatg ttagtatctt cgacaaatac gctgcaccag gaacagtttc
     3241 gacacaagga caaaagttca ttctgggagc agatgaaggc ggacgtgatg tattaggaca
     3301 attgatcata ggtgctagaa actctgttac gatcggattt gcgatcacga tccttacttc
     3361 gatcatcggg gttggattag ggatcatctc tggctactac ggagggatca ttgacaacat
     3421 cttgatgcgg atcgttgatt ttatcatgat tctaccgatc atgctgatca ttatcgtctt
     3481 cgtatcgatt atttccagat acaatgtctg gtcctttata ttcatcatga gtgcatttta
     3541 ttgggtagct aaagcccgat tattccgaag caagacatta tctgaagcaa gaagagatta
     3601 tgtcagtgcc tcaaaaacaa tgggaaccaa tgattttaaa atcatgttcc gcgaaatcat
     3661 gccgaatcta agttcattaa tcattactaa tttgacgatg aactttgcgg caaacatcgg
     3721 tatcgagaca actttgactt tcttaggttt cggcttgccg gcaaatgtac ctagtttagg
     3781 tacattgatc ggttatgcaa gtaacggaga agtcttagtg aatcggcaat ggatctggtt
     3841 acccgcatca atccttattt tagttttgat gttgagtatc aattatgttg gacaagcatt
     3901 caagcgttct gcagatgcgc gccaacggtt aggctagtat agaagcgaag ggggaaatag
     3961 gaatgaagaa aacgattttt ggatttgcag cgctgatgtc tgctgtcgtt cttgcagcat
     4021 gcgggggcgg aggtacagat acctctggaa acaactctgg aggcaacggc aataacaaag
     4081 aggaattaac ttttccaatt gcaacaaaaa ataatgatga tgcaatcgaa gacggcgaat
     4141 tagaagctgc ggtcgcaacg gacagtcagt ttaaaggatt gttccagtgg gagttttacc
     4201 aagattcata tgatgcagct tttatggctc cttctcatga accgctattt gcaaacgatg
     4261 acgatttcag aatcacagac gacggagctg caacgcttga tctagatgaa gatacgaaaa
     4321 aagcgacaat caaagtaaaa gacaacgtca aatggtcaga tggtgaagat gtaacggcgg
     4381 acgatgtgat tttcccttac gaaatcattg ggaataaaga ttacactggt atccgttatg
     4441 acgacacttt ccgtaacatc gtaggtatgg aagaatatca tgatggaagt gccgacacaa
     4501 tctccggaat caaaaaaata gacgacaaaa cagttgaaat cgaatataaa gaagtaaacc
     4561 caagcatgct tcaatcgggt ggtggtatct ggtcatatgc agcaccaaaa catacgttag
     4621 aaggtgtagc aatcaaagat atggaatcaa gcgatcaagt acgtaaaaat ccagtgactt
     4681 ttggccctta ttacatgagt aatatcgttg caggagaatc agtagaatac ttgccaaacg
     4741 agcactactg gaatggtacg cctaaattga aaaagatcac gatgaaatct gtaccgatgg
     4801 caagtgcaac tgaagcattg aactctaaat tatatgatat gattttccaa atgccaacag
     4861 atacttacga tacctataaa gaaattgctg gctatacaaa cttaggccgt gaacaaactt
     4921 cttatactta tctaggattc aagttaggta aatggaatgc agatacaaag agtgtggaat
     4981 acaatccaga ttcaaaaatg gctgataaat cattacgtca agcaatgggc tatgcattag
     5041 ataataatgc agtaggtgaa cgtttctatg caggtttgag aagcaatgcg acttcattga
     5101 ttcctccagt attccagtca taccatgatg atgagatgaa aggattcaca caagatctag
     5161 acaaagcgaa aaaactatta gatgatgctg gctacaaaga tgtggatgac gatggtttaa
     5221 gagaagacaa agatggaaat aaactaacaa tcaactttgc ttcgatgtcc ggtggggaaa
     5281 cagcgcaacc attggcagat tactatatcc aacaatggaa agaaattggt ttggatgtcc
     5341 aatacacaac aggtcgtctg atcgaattca attctttcta tgataaatta gaaaacgatg
     5401 atcctgaaat tgatgtttac caagctgctt ggaatacagg aactgatcca aatccaagtg
     5461 gactatgggg accaaatgct gcgttcaact acactcgtta tgaatcagaa gaaaatacaa
     5521 aattgatcaa cgatatcagt tcaagcaaag catttgatga agagtaccaa aaagaagcat
     5581 atcaaaaatg gcaacaatat gcatttgaag aagcatttgc gattccaacg ttgttccgta
     5641 atgaagtatt gccagtcaac gaccgtgtca aagactggga ttggtcttat gatgtaccaa
     5701 acccatgggc aaatgtttct gttacagcag attcacgttc ataatcacta aaaatgaccg
     5761 aaaaagggcg atgtcctttt tcggtttttt gatatttttc tgtttatttc tctaatattc
     5821 aggtcaaggc gctgatttca tgacttctcc aataaacggt gttcaaagtc cagtttctcg
     5881 gtattaagtc aaatttatgc aaaaatatgg aaagccattt tcacaaaatt ttttcttcat
     5941 actcgaaact aaacgaattt ttcacaactt ctcgtcaatc actaggatta ttaaagcttt
     6001 acttaccttt tgaccgaacc atttcaaatt tgacaaaaaa ccagtataat aaggaagtat
     6061 cgaaaaaata tccataaaag gaatgtggaa aatggacaac cagctgacaa aagaactaaa
     6121 agaaaactat aacatcgttt ttcacgatct aaatttgctt gaacaagctt ttacccattc
     6181 atcctatgtg aatgagcatc gcaatctgca attatcggat aatgagcgac ttgaattttt
     6241 aggagatgcc gttttagaac tgatggtttc tcagtattta ttccgtttat atcctcattt
     6301 gccagaagga aaattgacaa aaacacgagc tgcgatcgtt cgcgaagaca gcttgtcgaa
     6361 atttgccaaa gaatgtcact ttgatcaata tgttttgttg ggcaagggag aagagaattc
     6421 aggaggacgt acgcgaccag cattgctttg cgacctattc gaagcttttt taggcgcgtt
     6481 atatcttgac cagggattcg acacgaccca tgcattcatc gaaaaagtga tttttcctaa
     6541 agtcaaagca ggtgcttttt cacatgagat ggatcataaa acaaaactac aagaagtctt
     6601 gcaaaaatca ggtgatgtat caatcgaata ccgattgatc aatgaagaag gtccagctca
     6661 tgaacgagta ttttggatcg aagtttatgt agatgatcaa ttgattggca ctggacaagg
     6721 gaaatcgaaa aaattagcag aacaagctgc ggcggaaaat gcattggcag cactgtaaaa
     6781 gtaagtgagg gaaagctttg tatttgaaac gaattgaaat agccggtttt aaatcatttg
     6841 cagacaagac agtcattgat tttgaaaata gcgtcacagc agtggttgga ccaaatggca
     6901 gcggaaaaag caatattaca gaggctatcc gctgggtatt aggagaacaa tctgctaaaa
     6961 gtctgcgagg cggaaaaatg ccggatatca tctttgccgg ttccgatacc cgtaagcagc
     7021 tgaatattgc agaagtcaca gtgattttag acaatagtga tcattatctg ccattggagt
     7081 acaatgagat cagtgtaacc cgccgttatc gacggactgg ggaaagcgag tttttcatca
     7141 ataaacaaag ctgcagatta aaagatatcc aagaattgtt cttagattct ggattaggaa
     7201 aagaatcgtt ctcaatcatt tctcaaggaa aagtagaagc gattttcagt agtaaaccgg
     7261 aagaccgtcg tgggatcttt gaagaagcag ctggtgtctt gaaatacaag cagcggaaaa
     7321 agaaagcaga acagaagctt tttgaaacag aagacaacct cagccgcgtc caagacatca
     7381 tccacgaatt ggaagaacag ttaactcctt tggccgccca gagtgaggca gcaaaggagt
     7441 ttttacggct aaaagaaaca ctgactcaaa ccgatgtctc tttgatggtt gctgaaatca
     7501 agacagcaaa aaaagattgg gacaacaaac aagcacagct tgcgaaattc aatctagaat
     7561 taggcaaact atctgagtcg atccaagaac aagaatccat tttggcgaag cagcgtaaag
     7621 aaaatgcgca agccgatcgt ctgatcgaaa aaaatcagca agtattatta gatctttcgg
     7681 aaaaactgaa gcagacagaa ggacaaaaag atgttttgca ggagcggaca aagcatacac
     7741 aaaaaagctc gcaagaatat caaacttcat tagcagaagc acaaaaaaag gttaaacatt
     7801 ttgaaaagct tcaagaaagc ttgatgaaag cagcagcaga aaaagaaaca gagatccaaa
     7861 aagctgaagc aaatctcata aaaacacagc aagaattaga aaaataccaa aaatcaacga
     7921 aagaattatt ggcagaatta agagatcaat atgttgattt gatgcaagaa caagcagctg
     7981 taggaaacga actgaagtat ctagaacgtc aatatataca agaaacagca aaaagcaagc
     8041 agacgttagc caagcaatcg gaagtagaag catcagtaga ccgattgatc ttacaaaaag
     8101 aagaattgac acaaaagcag gcgcagctaa aatcatcttt aactgaaaca aaagagaagt
     8161 tagaaatgat ccaacaaaac gggaaaaagt tccaagaagc attagcaaaa gaacagccta
     8221 aaatgtatca attaatgaat caagtccagc agctacgtgc tcgtaagaaa agccttcaag
     8281 aaatccaaga aaactatttt ggtttttacc aaggggttcg attggtcttg cagcataaac
     8341 agcagctttc gggtattgtc ggagcagtgg ctgaactgat cgatgttcct gcagatttca
     8401 cgttagctat cgaaacagct ttaggtgggg cagcccagca tgtcatcgtt gaaaatgaaa
     8461 aagatgctcg gcaagccatc acgtatttga aacagcaaag aggaggaaga gcaacattcc
     8521 ttccactgac gacaatcaaa ccaagacaat taccggctca tatattgaca caagcagcag
     8581 ctgtagaagg gtttataggg attgccagtg aacaagtcag ttatcccgat caaatccaaa
     8641 ctgtggttca aaacctgcta ggaacgatcc ttttagccaa agacttaact agtgccaatg
     8701 caatcgcgca aacgatccgc tatcaatatc gagtggtttc cttagaaggt gatgtgatga
     8761 atgccggagg atccatgaca ggtggagcga ataaacgagg aaaccaaggc agtttgtttg
     8821 tacaaaatca agaattgaaa cagttgacta gtgaatttga agaagcagat aaacaactcc
     8881 aagcacaaga aaaaaaagtt caggagctgc aacaagaaac agctcgttta gccgaagaac
     8941 aagaagtttt gcgtacacga ggcgaacaat tacgttttga agaacaagaa gcgacaaatc
     9001 agctacaaaa catcataaat gaacttgaac gttttgaaaa agaaaagcaa atctcaactt
     9061 ttgagacacg tgaactgcag cagtttatcg aagattacga aaagcaaacg aacgaattga
     9121 aagacaaaca aacagatctt gaatcgcaac gacaaaaaat cgacgaagaa atcaaatcgt
     9181 taagtcaaga aagcgaccag atggaagcca gaagagcaca agtacaaagt caaaaagcac
     9241 aagaacaagc agatcttgct gtcttgaaag agcagtttaa tcatctgcag atccagctcc
     9301 gcggtgcccg agtccaaaaa gcagaggcaa ccgaacgtca agaagcgata gaaaagcagt
     9361 tggcgacatt gacagcagac ttttctgatc atgaagtcac agaagaaagt ttggaaaaac
     9421 aaattaacga attatctgct cagcgagaaa cattaaaagc cgagttggca aaagccaaag
     9481 agcagcgaga tagaaggcaa aaggagatcg ataagcttga agcagtcttg gcagagcgta
     9541 atcgtgagca aaaagcacga ctgtcggaac aatccaaatt agaagtccaa aaagaccgag
     9601 ctgaaatgat tttagataat catttgacct atctgcagtc agaatatcag ctgacatttg
     9661 agaaagcttc ccaagactat caagaaacaa ccgatatcga agacagtaga acaaaggttt
     9721 catccttaaa agaacagatt gaaaaactgg gaccagtaaa cttaaattca atcgaacaat
     9781 acgaacaagt cagtgaaaga catacattct tagcaacaca gcgagatgat ctgttagcag
     9841 cgaaaaacca attgtttgaa acgatggatg agatggatga tgaagtgcgg acaagattca
     9901 aagaagtatt tgaagctatc cgtcaagaat tcaaaattgt ctttccaaat atgtttggcg
     9961 gcggtaaagc tgaattagta ctgacggaac caactgattt gctgaaaaca ggtattgaga
    10021 ttgaagtaca acctcctggc aaaaaactgc aaagtctaag tttgctttct ggaggagaac
    10081 gagcacttac agccatcgcg ttgctctttt ccattatccg tgtctgtcct gttccgtttt
    10141 gtatcttaga tgaggtagaa gcagcgttag atgaagccaa cgtgaaacgt tttggtcgtt
    10201 acttgagcga tttccaagat gacacacaat ttatcgtagt cacacaccgc aaaggaacga
    10261 tggtcgcagc agatgtactg tacggagtga cgatgcagga atccggtgta tcaaaaatcg
    10321 tttctgtacg gatggaagat atcaatgaag aggcgaaaat caacgaaacg aaagtgtagg
    10381 aacgaaaatg atcaaattag tagcaatcga tttggacggt acattattaa atgaacaaaa
    10441 gctgatttca gatgagaata aacaagcatt agcacaagca aaacagcaag gggtcaagat
    10501 cgttctatgt acaggacgcc ccttagctgc gatggcgcat tatctgcaag aattaggact
    10561 ggtagatgaa ggtgactaca gcatcacttt caacggcggt ttggtacaaa aaaatgatac
    10621 tggtgaaatc atggaaaaga aagtgatgga agtatcagat atccaacgct tatacaaact
    10681 ggctcagcaa ttggatctgc cactagatgt tttgtctgac aatgtggttt tacagctgcc
    10741 tagtgcccca aggaagcaat ccttgtacaa tgtgttgaat aagctgctgc aattccaacc
    10801 agcggatata agcgatatta cagatgaatt tgtgcttaat aaggcagtaa tcgcttatga
    10861 ccaagaagaa ttagatccta aaatcaaaga aataccagca gaattccatg aaatgtatga
    10921 gatcatcaaa acaagaagta tgcttttgga atttatgcca aaaggtgtta caaaagctta
    10981 tggtatttct ttattggcaa aagatcttgg tttgaaacaa gaagaaatca tggcgatcgg
    11041 tgatgaggaa aatgatttac cgatgatcca atatgctgga ctaggcgtag cgatggagaa
    11101 tgctgtgcct ttcgtcaaag aagcagcaga tttcgtgaca gccagcaatg aagaaaatgg
    11161 cgtagccaaa gtgatccaaa aatttgtatt agagtagggg gagtcaaatg ggattttttg
    11221 ataaaatcaa gcgtgctttt actggcgaag agaaaacaga agaagttgaa gtacaggaga
    11281 agtacgacaa gggtttagaa aagacgcgta aaacatttgg tgaacggatg aatgagttgt
    11341 ttgccaattt tcgaacagtc gatgaagact tttttgaaga attagaagaa acacttatcg
    11401 gtgcagatgt aggttttgaa acagcgatac gaattaccga agcattacgt caagaagtga
    11461 aactcagaaa tgcgaaaaaa tcggcagaag tccaaaatgc gatcatcgag aaacttgtgg
    11521 atctctatga agcggaagga atcaatgaag taaatgaact aaatatccaa gaagacggac
    11581 taacagttat tttgtttgtc ggtgttaatg gtgtagggaa aacaacaagt attggtaaac
    11641 ttgcccatca gttcaaaaac gaaggtaaaa aagtattgat ggcagcagca gataccttcc
    11701 gcgctggagc aatcgaccag ttagttgtct ggggcgaacg cgctggtgtt gaagttgttc
    11761 gtggcaatgc aggaggcgat ccagcagctg tcgtattcga tgctttggaa agagcaaaga
    11821 cagagcaggc agatgtttta ctagttgata ctgcaggccg actgcaaaat aaagtgaact
    11881 tgatgaacga attagaaaaa ataaaacgag taatccagcg ggaattacca gaagcacccc
    11941 aagaaacact tcttgtatta gatgcaacca ctggtcaaaa cgcgatggtt caagcaaaac
    12001 aattcaaaga aacaacagat gtgaccggtc ttgttttgac aaaattagat ggtacagcaa
    12061 agggcggtat cgtacttgct atccgtaatg aattgcattt accagtgaaa ttggtcggtc
    12121 ttggtgaagg aatcgatgac ttggagccgt ttgatccaaa tgattttgtc gttggattgt
    12181 tcaaaggatt actaaaagaa gaataaccca tgtctaaaaa gcaggagctg tgacaaaagt
    12241 tgcagctcct tgtttgaatg attgttttta acatagctgt gtttttgatg ccttcatttt
    12301 tatacttatt tgtaagcggt tctcaaacaa tttcgttctt tttcttagac tttttgatta
    12361 taattagatt catatgaatt ggaggtcact taatgatgaa ttttaagcaa ttattattgc
    12421 atgtcaatgc gcgccctttt atcgaccaag ctcgatttgg tatagaacgg gaaggacagc
    12481 gggttgatct tgcaggaaat ctagcaaaaa ccgatcatcc agcaatcttt ggcgatcgat
    12541 cctatcaccc ctatatccaa acagatttta gcgaaacaca aacagagatg atcacccctg
    12601 ttaccgattc tattcccgaa ttatttcagt atctcgctgc tgtttatgat gtgactgctc
    12661 gttctatacc gaaagaagag atgatctggc cattaagtat gccacctgcc ttaccggaaa
    12721 aagacgaaga gatcattatt gcaaaattaa aaaatttcga agatgtcttg tatcgacgtt
    12781 atttagcaaa agaatacggg aagcgaaaac agatggtaag cggtattcat ttcaattttg
    12841 aatttggcga tgagttgcta agaacattgt tcagccatca ggaagaattt caagattttt
    12901 ccgaattcaa aacagaactt tatttgaaaa cagcgagaaa ctttatgcgc tatcgctgga
    12961 tgatcactta tttattcggt gcttcaccaa tgagtgagaa gaactacttt ttagatgaat
    13021 cacatccgca agaacctgtt cgcagtattc gcaacagtgc gctgggctat acgaatcatc
    13081 caaatgtgaa agtttcctat gcttctatga aacagtattt agcagatatc gagcgaatga
    13141 tcgaagaagg gaaactttcg gaggagaaag aattttatac accgcttcgt ttccgaggcg
    13201 gaaagaaagt cgcagattta gcaacaacag gtgttcgtta tattgaattg cggaatatcg
    13261 atttgaatcc ttatgcaaga ttgggtatca atccagagca agttcggttc ttacaattat
    13321 tcctgatgta catgttatgg acagaagaaa aagaagactg tgaccaatgg gtagcagaag
    13381 gtacgactcg aaataacaaa gtagcactag aacaaccatc agatcaaaca gaatttcatc
    13441 aagaaggtag agaaattctt gaaggaatga agcaaatgct ggttgaattg gattggttgg
    13501 attctcttta tttagtagag gaagcattga ctcaaatgga tcatcctgaa caaacgttag
    13561 cagcgaaact ctatcaagaa gcacagctat cgagtcagca agaagttgct gtggcattgg
    13621 gacatcaata ttataaagaa agccatgaac gtccctatca gttggccggt tttcgcgaaa
    13681 tggaactttc tactcagata tttatgttcg atgccatcca aaaaggtgtc caagtcaaag
    13741 tattagatga gtcggatcaa tttttgcgtc tgcaattcca agaccatgta gaatatgtga
    13801 aaaatgcaaa catgacaagt aaggacagct atatcgtccc acttatcatg gaaaacaaaa
    13861 cagtaacgaa aaaagtctta aaagaagctg ggttccgggt accaggaggc gcagaatttt
    13921 catccatgga agaagcggta aaagcttatc cgagatttgc cgagcaggca tttgtgatca
    13981 aacctaaatc aaccaattac gggttaggta tcacgatttt caaggaagga gctagtctgg
    14041 aagattatca agcaggttta gccattgctt ttcgtgaaga cagttcggtt ttagtagaag
    14101 aatttatgcc gggaacggaa tatcgtttct ttgtgatcga cggagaagtc caagccatca
    14161 tgttgcgagt ccctgctaat gttattggtg acagtatccg aacagtgaaa gaacttgttg
    14221 aagaaaaaaa tagtgaccct ttgcgtggaa cgaatcatcg tgcaccttta gaattgatcc
    14281 aattaggtga attagaacaa ttgatgttga aagaacaagg gttgacgatc gaatcagttc
    14341 cccaagccaa tcaaattgtt tatctaaggg agaactcaaa tatcagtaca ggtggtgatt
    14401 cgatcgatat gactgatgaa ttttctgaag cttacaaaaa aatcgccgtt tctgcggtag
    14461 aagcactagg agctaagatt agtggtatcg atttgattat accagataaa gaaatcgatc
    14521 ctacaactga taaaaaagca tatgggatca tcgaagcaaa tttcaatcca gccatgcata
    14581 tgcatgttta tccttttgca ggaaaaggca gaagattgac gatgaatgta ttgaaattgt
    14641 tatatcctga agtattttaa aagaaaaagc ttttgacaga ttgtcctatt tgatgtcaat
    14701 ctgatcaaaa gctttttttg catgtaaaaa tttatgtgtt ttttcgtttt aaaataaacg
    14761 atcccagaag ctttttttct tttttggttt ttctttttca gctggcgaag agttttcttc
    14821 tgggtatttt ttttcgatat caatcacagg ttcatttact gcttcttttg ctgttaagag
    14881 caatccgaat tcctcaggat ttgcagtgac gtaatcgttg actacagtga aacggctatt
    14941 gtttttcgtt gctagctgga tatattggtt ttggacagaa aggggtagag ccccattcaa
    15001 gagtatggaa gcatctggat gttttttgag ttcttttaaa aagttttctt tgttttttgg
    15061 ttggatcatt tcagcaacag tcatactaag atagcagcgt tccctgaatg tccctaaata
    15121 tttatgctgt tcttcagggt tcactaaagg tgtaccataa agtcccttat ccaaatggct
    15181 ttttacatca tcttgtgcca taaaaaatac cctccttctt ctctttgtct ttcttacgat
    15241 tatagcatat tcccaataat acctaaaaca tatgaaaaag cggttgcttt tgttcaacga
    15301 ttcacttaaa ataagaatga aaatcattct caattagaaa ggtggattgg atgcgctacg
    15361 gacgactagt agcattatta gttattttaa gtagtatttc attgttcatc ggagtatcga
    15421 atgtacaatt gtcagaactg ctccagttag atgggcaaag cttacaaatc ttgctagtga
    15481 ctcgtttgcc acgaacgatt tgtttgatcc ttgtgggagc gaccagtagt atttgtgggt
    15541 tgatcatgca acatttgacc caaaataaat ttgtttcacc aacgactgcg ggcacgatgg
    15601 atagtgcgcg tttaggtatt ttagtggcta tgattttcct acctggtgca tccttgattg
    15661 tccgatcctt gacagcattt tgctttgctt ttgcaggaac attgcttttt ttatccctta
    15721 cgcgattgtt cctccagaaa aatcaattga tgcttccgtt gattggagtc atgttaggga
    15781 atatcattgg ttcagttgct actttttttg cttatcaatt ccagttggta caaaatatga
    15841 cttcatggct tcaagggaat tttgcgactg tgatacgagg aaattatgag ttattgtatg
    15901 ctgttgttcc attgttgctc ttggtttggc tatttgctta ccgatttacg atcattggtc
    15961 tgggcgaatc gtttgcagta aatctgggaa tagattacca gaagatgcag tttttaggca
    16021 ttggcttagt atcattggct agtgcagtca tgttgatcat ggtaggaagc attcctttta
    16081 tcggagtgat cattccgaat cttgtctcta gaatatatgg agatcaagtg cacaaaacaa
    16141 ttggcgtaac tgctttagca ggtagtttgt tcttagtatt ttgcgatctt gtcgctcgcg
    16201 tgttgatttt tccttatgaa ataccagtca gtgtgatcgt tggaatcatc ggtggggcta
    16261 tcttccttta tcttctgata aggggacgac gatatgctta aaaaaaagcc atctattttt
    16321 attggtatgc ttgttcttgt tttatttgtt acaggaatgt acttgacgat ccatacatat
    16381 ggaaactggc attttgcatg tgcattgagg agtaagaaaa tttttgcatt tatccttgtc
    16441 tcgctagctg tcagcagtgc cacaatcagc tttcagacaa ttacgcataa ccagtttttg
    16501 actccttcag tattgggact tgagtcgtta tatgtccttg tccaaacggt ccttttcttc
    16561 tttatcggag gagtgacgat gcttcagcag acaggtacag tttttttctt attgaacgtt
    16621 gggctgatgg tcgggctgag tctattgtta tttttctttc ttttgaaaaa aatgagagcg
    16681 aatctttttt tattactgat gtcagggatg attttaggta cgctttttgg tagtgtcagt
    16741 acctttttac aagtcatcat ggatccgaat gaatatgatt tgcttcaagg cagattattt
    16801 gcaagctttg gcaatatcca gagtcagtat cttttgcctg cttctttgat cattatcggc
    16861 tgctggtacc tgttatttcg atatcgacat gtgctagacg ttttccatct cggaaatgaa
    16921 caagcagtaa acctcgggat agacacagat cgttttgaat ttttgatttt atgttgtgtc
    16981 agtcttttga cagggacatc aacggctctt gtgggtccag ttactttttt agggtttatt
    17041 gtcgcaaata tcagttatcg tgtcttttct acttatcgtc atggtcagct atttatcgga
    17101 agctttctgg tagctatgct gtttctgaca ggaggacagc tcgcagtaga gcagattttc
    17161 ggctggaata caacagtgag tgtcgtcgtt gaatttgtcg gaggaattta ttttgtcaca
    17221 aaattgattc gcgaaaggaa gagtatccta tgattgattt acaaaaggtc aataaactgt
    17281 accatggaaa atcagtggtt aaagatgtac agttaacgat tcctgaaaag aaattgactg
    17341 cttttatcgg tccaaatggt gctggaaagt caacggtgtt gtcgatgatc agtcgtttga
    17401 taccaaaaga tacaggtgaa atctatttag accataacga agtaaaggct tggaaatcca
    17461 atgaattagc caaaaaatta tcgatcctac gacaatcgaa tgaaataaat gtccaaatca
    17521 ccgtgaaaga attagtagaa tttggacgat ttccttatac gaaaggaaaa ttgacgacaa
    17581 aagacgagga aatcgttcag gaatcactgg aacaccttgg gttagaagaa atagcggatc
    17641 aatcgattga cactctttca ggcggtcaac tccaacgtgc gtatattgca atggtgctag
    17701 cgcaagacac ggattatatt ttactggatg aacctttgaa caatctagat atgaattttg
    17761 cggttcaaat catgcagatt ttaaaaaatt tagtacaaga aatcgggaaa acaattgtta
    17821 ttgttcttca tgatatcaac tttgctgcaa gttatgcaga cgaaattgtt gcaatgaaag
    17881 atggacagtt atttacgcaa ggaacgaccc aagaaatgat aaaaaaagat gtattggatg
    17941 aattatacga aatggacata cggatttgtg agattgaagg ccgcagattc tgtctttatt
    18001 tctaaaacta acattaggag agaagagatg aaaaaattag tagtaggtat cttgattcta
    18061 gcatcatttg gtttagcagc atgtggaaat agcaacaaca ccagtcaggc agacacaaaa
    18121 aaaagcagca cacaaactga aactacattg acgataacag atagtaatgg agaccaaatc
    18181 gaggtgccaa ataatccaga aaaagtcgtc gtattcgaca atggttcttt agatacgatg
    18241 gatgctcttg gtgttggaga taaggtagtt ggagcagcta caagcagcct tcctgagtat
    18301 ctttcatcct ataaaaaagt agaatcagca ggtggaataa aagaaccaga cttagaaaaa
    18361 attaatcagc ttcagccaga tctaatcatc atttctggcc gacaaagaga ttttcaaaaa
    18421 gatctaagtc aaattgctcc gaccattttc ctttctttag atgcgaaaaa tccatgggaa
    18481 tctttccagc aaaatgtcac tgctttaggg gagatctttg gtaagcaaga agaagctaaa
    18541 actcaattag aggaattatc tagtgcaatc gatcagacga aaaagaaagc agaagctact
    18601 gacaaaaaag cactagtcac acttgtgaat gaagggcaat tatcagccta cggatctggt
    18661 tctagattcg gtttcatcca tgatttattt ggctttgaac aagcagatga tcaaatcgaa
    18721 gcatccactc acggtcaaag tgtttcttat gaatatgtgc tggaaaaaaa tccggatatc
    18781 ttatttgtag tagacagaac aaaagctatt ggtggagacg acagcaaaga tgatatttct
    18841 gccaatgaac tggtagcaca aacgaatgct ggtaaaaatc agcagatcat ctcccttgaa
    18901 ccagatgtct ggtatttaag cgggggcggt ttagaatcaa tgaaactgat gatcgaagat
    18961 gtgaatcaag catttaaata gcataaataa cagaggatta aactctcata ctatgtaaca
    19021 ataatgtttc ttacattctt aaatagcaag agcctatcct caaagaagtt atcaaaaacc
    19081 atccgtaaaa tcgtattgat gattttacgg atggttttta tttatttgag aaatcttttt
    19141 tcaacgtgaa ttcattatct acatcatacg ggttgattct agaatgggga gtggcaaaaa
    19201 atagtgtgat ccattttttc agcatcaaga tcgcctcctt tgatttgaat caaaggtaca
    19261 aagcgaaact tagataaaac ttaaatttag atatttttat tttgtaccga tctcttcttt
    19321 aaacgatgtt ccatctgctg cttctcggca ataagtccac tagactagaa aatatgagaa
    19381 gctattttct gtccagcgtt cttattactc aaagcaaatc gcagttgtca caatctcttc
    19441 taaacgatgt tccatctgct gcttctcggc aataagccca ctagactaga aaatatgaga
    19501 agctattttc tgtccagcgt tcttattact caaagcaaat cgcagttgtc acaatctctt
    19561 ctaaacaatg ttcaatcagc tgaccttcgg tattagctca aagcactgat ttcacaacct
    19621 ctatttaggc atgtgtctca gcaagcggtt gatagttgat gatctgcatt tttcgataaa
    19681 aatcggcggg aagcttactc caccgtttta ataattgata gaaataactc tcttctgctt
    19741 tgattttata ctcgcttaat atcaatccaa aactattcgt tttttttgaa gctaattttt
    19801 tataaatatc caatgctttg gttttatctt cattgacaaa gagtgttttt acttcaatat
    19861 aagaaccctc gatattttgt gtttcttcga taaaatagat gttttttata tattccttca
    19921 tagtgattcc tccaaagaag tcttttattt gataagttta ctctaccgaa gagttgttaa
    19981 taatccgtga acaaaaagta attctttttc caatagttgt taaaggtttt ttatttttta
    20041 tagtattttt tgttatttaa gaaaggaata aggttaaaca aataacaggt tgctttttgg
    20101 gtctttttcc gttcaagaaa ctgaacgaag aaaagggaaa gttgtctctt ttgtgataag
    20161 agaaatcata ataaacgaat gacttacttt ccaataaatt gagaacagtc agaaccagac
    20221 aaattagttg catctgatga ataaagtgat tattctatga catgaggtga tacagatgaa
    20281 attagaagtg acaaaagaag cacaagaagt attgaagaat aaattagaag cagacgatca
    20341 acttttatta gatatcgaaa acggtgacgg tccatttgca gaaagccaag tcagttgcca
    20401 attggatacg gcatttcgat tgatcattgt caaaaaagat acacaaacag atctaagtct
    20461 ttattctgta gtggctgata caccagttgg accaatcaaa atcaaagaca gtgcaatact
    20521 ttacatggat gatccatcca cacttgcttt agaaccaact tataatagtt tacaattaaa
    20581 aggaccaagc ggcttgagaa aaggcaactt gcaagttgtt cgaaaagact agagggataa
    20641 aatgggaaaa ttggcgatca tcagcgacct acatgtggac atcaataaat tcggcgaatc
    20701 agagctgatg cagctttggc aagttttgca aaagcacaag atcactcggc ttcatctagc
    20761 cggagatacg gctaataaag tagaccgttg cctagctgtt gtcgaatttt tcgtcaataa
    20821 aggattatct acgacatttc atttcggcaa tcacgagttg gcagatgtgc ccaatgaaca
    20881 gatgatggac cattttccag acccacattt tttaaatgaa cggtatattg ctttaaatga
    20941 gcagaccgtt cttttaggga tgaatggctg gtacgactat cagttttccg aattgaaaga
    21001 ttacgaggca atcgtacgaa tgaagcgttt gtattggtat gatcggttca tccagcgtgg
    21061 gaaaacagat ctcgaggtca atgcagaaat gcttgcagct gcaaaaaatt tgctagacca
    21121 gctggaagca aaaaaacttg aggtgatttt atccactcat ttcgttccta aacgggcatt
    21181 tatcatgtat caatctgctc cttatgagcg ttggaacaaa ttaaatgcat ttttaggatc
    21241 agaatcattc ggagaattac ttgaccgtta ttcaaatatc aaacaagtag tttttggcca
    21301 tacgcatcgt cgctttgaag ataaatccat cggtcacacg atttacagct gcagaccttt
    21361 tggttattat tatgaatggc agctgacccg agattttgtc ttgaaagaac agttgctaga
    21421 cagctatgat cctatgaaat tacgtgtcct cttgcgtgcc catcacccag cttttcaggc
    21481 ctatcaaaca caacatttgc aagaggaatt tgaacaagcg atgacaatcg ttccgtattg
    21541 agacagcatt agtaatgtgt aaaagaaaat accttgtaaa acataaggat aagtaagagc
    21601 agtagatcca tttatcgtgg atatgctgct ttttttgcgg aaaataatca gcgaatgttt
    21661 atttgatcgg atgatttgta actcgcagag tactagatgc aagcgtgtat tctttttttg
    21721 aacgataatc acaaaaaaaa atataatata cattttttat ttaattataa ttaatattga
    21781 ttgaagtcta ggcagtcaag tcacattttg atagatagga gggggactta cgaattattt
    21841 taaaggctat gacagctgta tttctagatt gtgtttgcat caagttgcaa taaaaactag
    21901 aaatcaaatc gggctgataa tcatttataa ctaatcgata cataggacca aatataggtc
    21961 tatgttcttt tatttcataa gtatcgtaaa acagaatttt gctatggatt aatccaatcg
    22021 actgatatgt ttagtaaata aagaaagagg aatgtcaatg atgaaaaaag aaatcgtatt
    22081 aacagcagca atgatgttat tcagcatggt agcaagtacg acttttgttt ctgcaactga
    22141 agtataccca aaggaatata acacagaagg aacgattaca tttgaagcag gagatgaggg
    22201 tgtaactcct cctgttgatc cagagaatcc tgatcccaat aaacctgtcg atccatcaga
    22261 tcctccaagc cctggaacag gaggggcatt atcgattgat tatggatcaa aattcaaatt
    22321 tgggactcaa aaaatttcta ctgcggataa aacgtattat gcagctgctg atgtgatgaa
    22381 cgatgggtct agaaaaccga catatgtcca agtgacagat aggagaagta ccttatctgg
    22441 gtggaaatta agtgtctctc aaccagaaca gtttaaaact gccagtgggg atgaactagt
    22501 tggagcacag ctgaaattta cgaaagggca ggctgtttct ttggttgatc caacatatac
    22561 gccgcaaacg gtgaattctg aattaacgtt gacaccggga ggaaacaata cgttagcaat
    22621 taatgctaaa tcaggaacag gtgtggggac ttgggtctat cgatttggtg caaatgaaaa
    22681 tgaaaatcaa gatgcggtac agctttcggt acccggtaaa tctgtcaagt tagcgcaaca
    22741 atattccact aaattagttt ggacattaga agatacacct aacaactagt ttactgaaaa
    22801 gaactgttaa aaataaatac gtagtcatat tgtcttgcga ggtaatcgag caaaaattgt
    22861 caaaggctag gctaaatttg tttggatacg aatagttcat ctaggaattt aacgatcagg
    22921 aggaaaagat atattgaaaa agaggaggat cgtgctcatt ttaagcagtc tcttagtgac
    22981 taatatcttt ccgatcatag ggtactcttt ggaattgaat cagttggagc atggaaatag
    23041 aagagacgaa gaaagcaaac tcacttcgac attcgacagt ttaatagaat ccaaccagac
    23101 acaagaaagc agtgaggatt taagtatttc gagtagtgga gagaccactg acaacgtaga
    23161 agctgagggt tcggacaaac aaggactaga agatgcacca atttctgcgg ttgagctgcc
    23221 tgacgatccc aatattattt ctatcgataa aatttttcaa acacctatcg gtactagcac
    23281 gtccatattg gaaaatggga agttactgca gttgaaccca gcagaaaggt ctcaaagagg
    23341 agctatttgg tcaaataaga agattagttt actctcggat tttacattta aatcttattt
    23401 atacttagga aatcaacgag atagagcggg agatggcatg acctttacat tgactggcga
    23461 tcctagaatg gctagtaatc ccagtcaagt tattggatcg ccaggtatgg gaattggctc
    23521 atattctact cgttctggcc aaccttacat tgcgaatgcg ctctcgattg agtttgatac
    23581 gtataaaaat agtggttctt ctgatcgaat ggatcgagag ataactgctg ataatagagg
    23641 gcatggacat gtcgcttttg tcacgcctaa accaaataat aataattatt ctggagagca
    23701 tactggcgtg acggtagctc ctacatttct ttctaatggc acatggcgct tgttaataat
    23761 taaatgggac gcggagcaaa gaagtcttac ttatgatttg gaaggtgtcg gtcactcttc
    23821 tcggattata cctgatctca ttaggcagtt tggttctaca gaagtaaatt gggggtttac
    23881 ttcttcaaca ggtacttttt ttcaagaaaa tgctgtcgct gtcacgcgac ttccatcgaa
    23941 tgtcacatca acagctgatg taaaagtcaa cgatagtgat tatggggcca tcgctgaagc
    24001 tggaggaaac gataagataa ctatcagaaa tacattgaag gtcgaggggg attttcttga
    24061 atataagaaa atgcagacta gtatcgacct cccacaagaa ttaactattc ccgagcatat
    24121 cttgatcaat ggaaatcaaa ttgatgccaa agatatcttg ataaccggaa atcgagtcac
    24181 gctaaatctg acagattatt ttattgctgg agatacgtta ataatcgaat tggagacaac
    24241 tttgcaggaa gtaattcctg aaaaagtact cactatgcat tttgaatacc ttgaaaacgg
    24301 gaatttgatt gagcaatcga atgaaataaa cctaaagatc gcaaagctga aggagataga
    24361 tatccatgtt tattataaag ataaagacag tcagattgat attgctgaaa tgaaaacaat
    24421 cactgggaaa ataggagaga agtataaaga aactcctatt gatattgaag ggtatgtttt
    24481 tgactcggat tcagcgaata cagaaggcat cttctccgaa aatacagagg atatctattt
    24541 ttattacaga aaaggcaaac tttatctctc aactgctcct caatatgtca attttggaaa
    24601 acataaaata tccagtagac cattaagtga attcggacag ttttcaggtg ggttgaaaat
    24661 tatagatgaa cgggcttttg gaagttggaa attacaattg aaacaagaga aagttcttac
    24721 aaatggagat tttgagcttc cagaagctct ttcctttgtt acctctgaaa atacaacggt
    24781 tatagggaca tccgctgtta cgattttcga aagtgatcaa aagggtgaaa gcgatttgtc
    24841 ctctctttta gattctacag ctcatcgtgg aatcagaata gatattcctg tagaaaacca
    24901 aagaatcgga acctttgagg ggaaactatc ttggatactt gcagatgtcc caggaaatta
    24961 acaggaagag aagaatataa caaaattatt gagtttcaag aagggcacag gtaccatagg
    25021 ctcttgtgtc ccacagagac aagattttgt tatagtcttc tcttacatat gattaaagga
    25081 gcgattttct tgagaccaaa acaacaatta tttataacac tcctagttat tttaggggca
    25141 ctttttaaca atatatcagt gtcagctagt gcatttaatt tttcagtgac gccgataact
    25201 tcagagaatc aaatcgacaa gagaaaaact tacttcgatc ttcaacttga tccggatcag
    25261 gaagtagaag taaaagcgga attgagaaat gatacagaaa aagaagtgaa aatcgatatt
    25321 tctgtgaata gcgcaaccac caattcaaat gtgatggttg aatacggaaa aaacgagatt
    25381 gaaaaagaca tgagcctgat ctttgatctg gtagattatg tgagctatcc acagatagtg
    25441 acgctaaaac ctaaaagtgt ccaaaccgtt gcttttcatg tcaggatgcc gaatgagcga
    25501 tttgatggtg tattggctgg tgggatcaca ttccaagaaa tccagacgga aaaggaccaa
    25561 acagaaacta aggatcaaag cttatcgatt gagaatgaat acacctatat tattgctttg
    25621 ttgatgcaac agacactgaa cgaagttgct ccgaacctta ctcttcatga agttaaacca
    25681 gatcaaatca atgcaagaaa tgtcatctta gcaaacgttc aaaatgatca aaaaacctat
    25741 atcaatcaag tagttattga aacaaaaatt acaaaaaaag gacattctga agttttatat
    25801 caagaggaaa aagaaggatt gcagattgcg ccgaatacaa atttttcttt tccaactgct
    25861 ttaaatggtc aaccgctaac cccaggtgaa tatcacctta caatgactgt tttaggaaat
    25921 gaaaatgaaa at
//
LOCUS       DAZUMK010000030        25665 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000030 DAZUMK010000000
VERSION     DAZUMK010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.030"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(293..1603)
                     /locus_tag="ACG808_001191"
     CDS             complement(293..1603)
                     /locus_tag="ACG808_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFS5046831.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1615..3033)
                     /locus_tag="ACG808_001192"
     CDS             complement(1615..3033)
                     /locus_tag="ACG808_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5046832.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3273..4841)
                     /locus_tag="ACG808_001193"
     CDS             complement(3273..4841)
                     /locus_tag="ACG808_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFS5046833.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4845..5255)
                     /locus_tag="ACG808_001194"
     CDS             complement(4845..5255)
                     /locus_tag="ACG808_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFS5046834.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5252..6175)
                     /locus_tag="ACG808_001195"
     CDS             complement(5252..6175)
                     /locus_tag="ACG808_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFS5046835.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6172..7050)
                     /locus_tag="ACG808_001196"
     CDS             complement(6172..7050)
                     /locus_tag="ACG808_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFS5046836.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7037..8374)
                     /locus_tag="ACG808_001197"
     CDS             complement(7037..8374)
                     /locus_tag="ACG808_001197"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFS5046837.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8377..9480)
                     /locus_tag="ACG808_001198"
     CDS             complement(8377..9480)
                     /locus_tag="ACG808_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS5046838.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9554..11062)
                     /locus_tag="ACG808_001199"
     CDS             complement(9554..11062)
                     /locus_tag="ACG808_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS5046839.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11028..11720)
                     /locus_tag="ACG808_001200"
     CDS             complement(11028..11720)
                     /locus_tag="ACG808_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFS5046840.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11742..12716)
                     /locus_tag="ACG808_001201"
     CDS             complement(11742..12716)
                     /locus_tag="ACG808_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5046841.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13016..13888
                     /locus_tag="ACG808_001202"
     CDS             13016..13888
                     /locus_tag="ACG808_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5046842.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14346..16544)
                     /locus_tag="ACG808_001203"
     CDS             complement(14346..16544)
                     /locus_tag="ACG808_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5046843.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16776..17768
                     /locus_tag="ACG808_001204"
     CDS             16776..17768
                     /locus_tag="ACG808_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5046844.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17828..19249
                     /locus_tag="ACG808_001205"
     CDS             17828..19249
                     /locus_tag="ACG808_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFS5046845.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19242..20741
                     /locus_tag="ACG808_001206"
     CDS             19242..20741
                     /locus_tag="ACG808_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFS5046846.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20780..22174
                     /gene="uxaC"
                     /locus_tag="ACG808_001207"
     CDS             20780..22174
                     /gene="uxaC"
                     /locus_tag="ACG808_001207"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFS5046847.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22333..23016
                     /locus_tag="ACG808_001208"
     CDS             22333..23016
                     /locus_tag="ACG808_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFS5046848.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23018..23854
                     /locus_tag="ACG808_001209"
     CDS             23018..23854
                     /locus_tag="ACG808_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFS5046849.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23865..25619
                     /locus_tag="ACG808_001210"
     CDS             23865..25619
                     /locus_tag="ACG808_001210"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5046850.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKNQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
BASE COUNT         7825 a         5210 c         4499 g         8131 t
ORIGIN      
        1 ttaaaagttt ttttcgattt cgtttttcga aatctttcag caactcattt atcataactt
       61 atcgtttgtt atttgtcaag aacttttttg aagttttttt cgatagaatc aagcttcgca
      121 agtggttctt tttaacgact tagttatctt atcacgtcta aaaacatgtg tcaactcttt
      181 tttggaattt tcataaaatg tttcaatgaa gcgcgacaac gttgtttaat atatcattaa
      241 aaaaaataaa ggtcaagcta aaatattatt taagcttgac cttattataa tttcaacgat
      301 tgtcaaaagt attgtttgca gctgtgttgc cagtttctat agataaatca cagcttttga
      361 aacataagtt ttgtactgct tcgattcgcc cgaattcttt tgcagttatt tggcaatgag
      421 aaaagcagac atcttctata ggcttttcag gaaatgctac taaatcaaaa gctcgggaag
      481 gaagtgagta acctggagaa aggcatgcct gtacgttttt gatgtgtatg tctttgactt
      541 tcgtcatttg ctttgattca ggtatttctt cagccactgc ttgccaactt tctggcattt
      601 gagcaaggtc attgctattt tttctattgt attcattatg ccagtccatt atccaagaaa
      661 aaggaaattg tacatcaatc atattcagat tttctaccct gatattttcg atcactcccc
      721 ctctttcttt tgaagatttc attcgaatac cacagtctga ctgaaaaaaa tcgatatcgt
      781 gaatatatac gtcactaact ccagcactaa cttcgctacc aatagttaca ccgtatcctg
      841 aacggatttt acatctagca acctcgattc ttgatgattt tcgattgact cttgcaccat
      901 tgccatctct gccagactta atagcaatac aatcatcacc acaggccaaa tcacattcat
      961 agacacgcac atttgttgaa gaatcaatat caatcccatc tgtacttggt ccatcattgt
     1021 tgcggatgat tactttttct actaatacat cgttagaata agtgatttgc aagttccaaa
     1081 aacctgattt ctgaaaaatc aaatcacgaa caacaacatg ttctgcatga tacagtaaac
     1141 aagcgcgtgg acgtttaata gcataatccg caatccaacg aagtcccttt tgatcgtatt
     1201 cagctcttgt cccgcttttt tgatcttttc cccaatacag ctcccaccaa tggtctcctt
     1261 gaccgtcgat tattcctttt ccttctataa gaatatcttt cgccgagaga acattcaaaa
     1321 tcgctgctgg ccatttcatt tctacgccgg ctactcgtgt atcgatttct ggaaatgctt
     1381 cgatatctgt tctccccttg aggactgcac cttcttcaaa gataagatga ctcttatctt
     1441 ttaaaaaaag ggctccgact aaataaatgc cttttggaat aaatacaggt aagtgttctt
     1501 tcgcaccttg atcgattgct ttttgcagta ttattgtatc atcttgttga gtgtcgtttg
     1561 ttttaaagtt caaagcattg ataatccctt tttttgtccg caatacgttt ctccctatct
     1621 ttcattgatt tttttcaatt cttcaccaat aaatacagtt tttgaacgat tcagtgggta
     1681 acctactagc aaaatgattg cggatacaaa taagcagaat gctggtaaca agtttgccat
     1741 cgtataaatc ctttcttgta ctaaaactga ttggatggct ccacctgttg tagacgattg
     1801 gtaaccaatg agtgccagca taaatccgcc taccgctcct gcacatgctt gtcctacttt
     1861 tctcgcaaaa gaatttactc catatactgt tccatcttct cttaagcctg tcttatattg
     1921 atgataatca atcacgtcag taatgaatgc ccagaccatc aaattgaata aaccagatcc
     1981 taaagtagcg ataaataaaa gaatcagata tacccatgca tttgttatat gaaggaaata
     2041 catcagcaag tacatacatg aagaagcaag taacgcaaat acgcttgctt cctttttacc
     2101 aaataatttt gtcagctttg tagcaaatgg tgctaaaact actactgttc cgtaagtaaa
     2161 taaaagagca acagacattg cttgtttatt atggaagtaa tcattgaata aataagtcat
     2221 attcgtccca gctaaaatct gattgatcac aatgaaaatg tcaacaatga ctaaaataat
     2281 cagtgctcga ttagctaaca aaccttttac caactgagaa gcagatactt tttcgttttt
     2341 gacaactctt actctttcag tagtcaattt acaagttaac atatatgcga caaatgctat
     2401 tgcagcacat ccgattgaaa tccaaaagaa atgattccct gaaacgactt gttgtccact
     2461 cttgttatgg gtatacatta aaatcggaat gaagaatcct gtagttgatc cgccaatcgc
     2521 agaaccaatg cttcggaacg tggacaacga ggtgcggtca ttaggatttg agctaattgc
     2581 tgctgccatt gctccatacg ggatattgat tgttgagaga aaaatcccat aaacaatgta
     2641 tgaaacaaaa atataagcaa tcttcaccgt cattggaaga tgttggacga atggcaaaaa
     2701 caaaatgaca gtcactaagc aaaatggata cttcattttt ctgatccaag gaataaatcg
     2761 gccagtttct gttaactctg agacatcaca taatcttcca accatcacat cagcaaacgc
     2821 atccaaaatt ctcgaaatca agaataacgt acctacgatt gcccctgaaa cgccaagaac
     2881 attcgtataa taaatcatca agaaactatt gactaaacct aaaataaaac agttacctag
     2941 atcaccgaac atgtagccta tcttatcgga aatcccaaat tccttttttt ctttttttat
     3001 tgtatttgct gtaacagcat tttgtgaaag catttgaatt cctcctacct cttctagtaa
     3061 acgttttcaa aattataact ctttttttct tgtttttata ttcacaatgt tggcttttta
     3121 ttgcattatc ttgacagata aaaaacaaaa aataatctaa aaacattgat gcataagatt
     3181 gataactttt tttacataac aatatataca aacaaaaaaa ttcactaact agtgaaaata
     3241 agttagtgaa cctcatttat taaagaatta aattacagat gatcttttcc atcatacttg
     3301 aaaaactcaa aatcagcata acttcctctt tttttattta gatcatgcac tgctatcccg
     3361 ataaaatttc cagtaaaccc tcctgagagt atacacatgt ttttttgcgg acctactttc
     3421 ttccattctt cagttgatac gtctttcaaa tgaaactgtg ctgtctcttt ctttacagtg
     3481 actttcaaaa aagtcttctt ttctgtgagt ggaatcgtcc aatcttctat ttgtgctact
     3541 ccgttttctg attctatcat acgtaggcat tttcccttgt cctcattcca agtaacataa
     3601 caataaatat aattggattc atttaagtac agtaccagtc cagccatctg attaaaatta
     3661 ttaggcatgt attcaaatgc tgtctccgca tcaaaagtaa aatccgtttg ccgaatagct
     3721 aacaaatgat gttcgaaggt agattgaaga gattcaccgg aaatcagacg taaaaaacct
     3781 ggtctacatt ttgtatcaca ccaatcagca tttggcaaaa tgcgtctggc attccagttc
     3841 ttctttagct ctccttcaaa tgtatccata aactgatgat ctttttcttg ttgtacctct
     3901 tcatttgtct caatcattat ttcttccatc ggagttgtct ggctatcttt aagtcttaac
     3961 caatcgtcgg acgtccaata cacttcttga atagcggttt ctcttcccaa aatagccgca
     4021 ccgtttaatg gtctggtaca aagatatgtc atgtaccatc ttcctgacgg ggtttgtact
     4081 aagctcccat gtcctgtaca ttgcaatgta gaagcaggtt tgtcactagc tgtcagcata
     4141 ggaaaaccag gatcaagctc gtaaggacca gtaatattct ttgctcggca aactgtaact
     4201 gaatgtcctc ttcctgtacc gccttctgcc gtcatcaaat aataataccc gttacgaaaa
     4261 tacaaatgtg gtgcctccgt tttcgctaat tctgtacctg caaatatttt atagatggga
     4321 ccatcaagct tcttcttctc tgcatcatat tcttgtaata cgataccgct ggatttattc
     4381 ggtgtgtcca gacgataatc ccatagttca ttgacaagcc atttttttcc cgtttcaaga
     4441 tcatggaaca aagaaggatc aaagccgtta ctatttaaat aaattggttc ggaccaagga
     4501 cctgtgatct cagatgcagt gatcaaataa ttgtggacat ctttaaacgg acggcctgca
     4561 cttttgacat ctgtgtaaac aagataaaac aatccatcag cataactgat ctgtggcgcc
     4621 catatacttc catttgtagg atttccttgc aaagatactt gagtggtcaa aatatccgtt
     4681 gtatgtgacc agttgactaa atttttcgac tcatatactc ggattcctgg aagccattca
     4741 aaagaggaga caataatgta ataccgctct ttcactctta atatattagg atctggattg
     4801 aatcctctta aaataggatt tttcactttg atagtctgca tgattcaggc tccttttctc
     4861 attcttactc gatataaact gaatgcccca gctgcgaaat aaaaaaatgc aaaaggaaac
     4921 gcaataaact gagtgccaat caacaatatg aatcctgtta taaaatacaa accagtggca
     4981 gttgttaagt attttccttt agcagtcaaa aacaatgcta ctgctaaaaa agcaagtgaa
     5041 ataaacaaca caacggcaaa cagaccagtc atgtcctgta ataatccaaa tgtattttct
     5101 aaagacaaag ttgaatgatc tacagcaagc gactgataac tttttttaaa ctctgacgca
     5161 gacatattac ttaaagtaac tgctaacccg ccaaagaaaa tcaaagatag actgccaccg
     5221 accattccta gcaatagttc ccattttctt ttcatttctg aaccccctgc ttttctaaat
     5281 gtatccattc aggtcttttg atcgatgttt cataattgtt attacgttca gagaatttga
     5341 cagtctgttc atttttcttg tcatcccaat tatcccaacc agttgggtga acatgcccac
     5401 ctatcttaca cgcttcaaat aagacatttg catacggacg ccacggtctt cctaaataga
     5461 cgtttctagt atcaggttct gcagtcaagg tacagctttc aaaacgatac cctgtttttt
     5521 gatttttcgg cgtagatgct gctgtgatgt atccaggatt tttactggct aagctatgga
     5581 tatgacactg atgaaaaacg gcatctgccc caccaaatat gaaatccacc gttccttcaa
     5641 tccagcaata ttcataggtc tgacgacaat actcaggatt ggcagaagcc ggtgtacaaa
     5701 aaggagtacc atctttttgc aaatcaggca acggacccgt gcataaggta tcctgataac
     5761 cagatagtcg acagtttttt aaagatacgc gatctcctga attgaataaa gcaacagcct
     5821 gtcctacctt ttctcctgga cctgctgtat tttcgatgtg aagattttct aatacgacat
     5881 tcgatccttc taagaaaaaa gtagcacttc ggaaagtccc taactggcta ccgtcaagat
     5941 actcctgttt tgcaaaacga ttaccggaga taactactgt tcctgctccc acaagtttaa
     6001 aattggataa tctgctttct atatattcat catatcttcc ttcttttact atcaagacct
     6061 ttggatggct atccggcagg cctgccaaat aatcgattcc ttcttggata gtttcaaaat
     6121 ccgtcggggt atctatagca ttctttccaa ttatcagttt gtctttcatc ctcataaatg
     6181 ctcccttatg aattgtttcc aaatatttct catttcttgt gtttccatat gaccgcctgt
     6241 taaagaaaaa gaagaaaagt tttctggact acccattgat tgatactttt tgtccagccc
     6301 ttttctcagc ttcagtacgc cttcgtccaa gcacatcgta tcgttttttc caactaagct
     6361 aagacgaggg cgcggagcga ttttttcttg gattgctaac gttgtataag ctgtcaatag
     6421 atttggcaca tagtaataaa acccatgatg atctaggcgg cgatgtagca aaagtatttc
     6481 gatgtccacc tgtgccgcta gatcaataca aactttgaca cgagaatcca atgctgtcac
     6541 ccaccagctt aataagcctc ccatagaaag accaatcgtt gcgagccgtt gagtatctgt
     6601 gtcttccctt gtctccaaat aatcgatcaa gctgatcaca tcataaatac gcattcccca
     6661 taacgttttt cctgttaata aaaattgttt gaacaactcg ctttcagaaa tacctccgcg
     6721 ttcttcaaat ccccaagcat caatcgacca aacagcatat cccattcctg tcagtacttc
     6781 tgcaaaagat ggagaagcta agtaaggagc accatggatc aattcacttt tcccgactga
     6841 aaaatctccg ccgtgagaat gcaggtaaag gaccggcgga aacggtcctt ctttttctgg
     6901 ataagcatag agggctggta ctgtttccaa ttcatttaag tacaattgat agtattcaag
     6961 cataaaaccg ttttgttttt ctcggtataa tcgttttcca ctaggggtat gtctgctaga
     7021 tggagcacct aaaaaatcat tcaactgcat aagcaaagtt tttacgaaca accagttcat
     7081 ccatattttc tgattgattt gattggaatt tctcaatact gatttcttta gtattttcta
     7141 atagaaatgc tggtccctcg tgattttcaa tcgacatctg gttaaaggaa accttacccg
     7201 caaagccaat ataaaatcct tgtctgctca tttcctctat tcctgtcatc atagcaggaa
     7261 caccgggaac tgcatcttgg gccatcgaga catcgatatt gtcaaatgtg atttcttgta
     7321 tgaattgttc cgctaatcca taaataaaac ctgcagaagc atggacattc cttgccgtga
     7381 tattcgaaaa atggatcctt ctgaatgctg gtgtccgttc atctatcgga taagcctttt
     7441 tttcccatac atacggttct ttccctcgcg gaccacagaa ataataaaga ttcagtataa
     7501 atggacacat cacattatcc atgactatat tggatactcg aatatcttca acgatcccgc
     7561 ctcgacctcg tctagatttc aatcgtattc ctcgatcagt ttcttggaag atacaatttg
     7621 aaatggtaat attacgaata ctaccactca tttcacttcc taatacgaca ccgccatgtc
     7681 catggaccat cgtgcaattt gtgatcgtga tattttcgca agcgatcctt tcgtacgtat
     7741 cttcagtccc tgccttgatt gcaatacagt catctcccac gtcaatatgg caattactaa
     7801 tacgcacatt tttgcatgat tctggatcaa ttccgtctgt atttggtgaa tccgctgggt
     7861 tcaagatcgt caagttatca aacgttgcat tactgcaaag aatcgggttg attgtccaac
     7921 ttggtgactg gatcaattta atatctttca cagtaattcg atgacagtta tggaaactca
     7981 tcaatttagg acgaggataa gcgagattat ctggttcatt tctaaaagta tgccaccatt
     8041 tttttccatt tccatctaat gtaccgaaac cagtgacaga gatgttctct acattttgag
     8101 catagataca ggaagcataa acttttcgat ggactccttc ccaacgtgaa tgaacaacag
     8161 gataatcttt tggatcgtca gagaatttca gaaccgctcc tgcgcttaaa tgaagttcga
     8221 cattgctttt taaaaataaa gctcctgtta gaaattcacc tgctggaaca actacggtcc
     8281 cgcctccgtc acttgctgca gcatcaatgg cttgttgtat tgcttcagta ttcaattcat
     8341 caattgatgc gccaaatgtc aaaatatcat acatatttat aatgcctcca cttcaatcaa
     8401 tgcttgcaag aatgcaccaa cgcctttttg atcattacaa ataattggtt cactgatgta
     8461 gtaagcatat gaaccatctc gctgatctgc tccgcctaac cctgctactt gacagttttt
     8521 attcaagttc agccagcctt cttttgtttc cagaacaaat tcatcaagta atccttgatg
     8581 tgatttcttc acttgtggaa gccattcttc tttgttcagc agtcccaaac gtattccttt
     8641 agccattgcg taaacaaaca tactactgcc ggaagcttcc agataatttc ctttccgttt
     8701 tgtttctgtt gttacttgat accatgtccc gctgttttct tcttgatatg gaagtaatgc
     8761 agccagtgtc ttttctagaa tttctttcag caaagaacaa tctgtttctt catgaagtaa
     8821 ttcataggta tctgctgcag ccatcaaata ccagccaatc gatcggctcc aaaaatgatg
     8881 ggatagacct gtttttggat cagcccatgg ttggaccttc ttttcatccc atgcatgaat
     8941 cagcaaattt gtttttggat cgaatagatg tttataactg atttcaaact gtctcaaaac
     9001 atcttggtaa tctttttctt cactaaaatt ctggataaat tcagcataaa aaggagaacc
     9061 catatataag ccatccaacc aaatttgata aggataaatt tctttatgcc aaaaagctcc
     9121 ttcggatgtg cgtggatggt ttgccaactg ctgggaaagg agttctgctg cttttttata
     9181 tttttcttcg cctgtttctt tgtacaataa aaagaaaagc tttcctgtat ttacatggtc
     9241 gatattgtat tcttcaacgc tgtagccacg gatcgttcca tcttcttgca cgaaatagtc
     9301 gatattgtct tttatgaaat caaaataaat tttttcttgg gttcgttccc aaagtaattg
     9361 gaacccttta agtacgacgc catagtcata agaccatttt cctttatacc acttttcttc
     9421 atataagcga ggtgttcttt ccatgatact ttttgctaat ttctttgacc agttttccat
     9481 tatttgctct cctttaaaaa ttaaaatagg atttgggaca aataccaaga agtatttgtc
     9541 ccaatcgtct atattactgt gccgctgctt tgtatgcttc ctcgtactct tgtgtgattc
     9601 gatcgcctcc ttgtgctttc cagtcttcta cagctttttt gaatccatct tcgtcaagct
     9661 gtcccatgat gtattgcacc gtagcatcaa tgacgatttt ttcaatttct gatcctactt
     9721 cattgttcgt cggtgaatcc aatggcacag aaggatccat tacagcaaat tcttcatttt
     9781 ctttgatcaa ttgattagag aattcttttt cagggtctgt atctttgaaa gagaacgtca
     9841 cttcactagg acgagagctt gataacggtt gcacctcttg ttgccaaaga tccatatcga
     9901 taaattcgat agaaccatca tcattcaatt cataatgcgt tccttcgata ccgccagtca
     9961 tcaatttata cacatcttct tctaaaagat catcaacaaa ttgaagtaat ttcttcaatt
    10021 gtgcttcgtc ttttacttga gatttgggaa atgctagcaa gccgccaata ccatttcctt
    10081 ctgaccaaac atgataaccg ccgccttcac catctgagat cttgtttaca ggcactaatt
    10141 ccatatcatc ttgaatacct tgacccatcg tcttcagatt cttgatatcc accattccag
    10201 tgtagattcc agttcttcct tgtgcaaatt gttcttgctg atcagtcttt tgggtaactg
    10261 caaagtcttg tgccaaatag ccattctcaa atagatctct tgaaaaattc aaagctttca
    10321 tatactcttc tgtatcaaat tctggagtga atttgccatt atcgtctaca gcccagccat
    10381 ttggtgctcc aaaataagaa gtgaaaagtt taaagctggt ataacgaata tcactaggcg
    10441 cacgatcgcc aaaaccaact gtgtcgtcca caccatttcc atcaggatcg ttttctgtaa
    10501 attgtttagc tacttcataa agctcatcca tggtagttgg aacatctagt cctagattat
    10561 ccaaccagtc ttttcgaatg actaagccag cacgagcgta gtctttttgt atcggtacgc
    10621 cgtacaaggc accgtcgata ctcgcagctt cgattctatc ttcagaaatt tgacttaaat
    10681 ttttatagtc ttttaaatat tctgatacat cccagaatac gcctgatttt aacgcattgc
    10741 ggacagaaga gtttttcaac atcgcaattt gaagtgtaac gatatctcct agttcatcag
    10801 aagccaatgc agttgttaat ctttcttctt ttgaagcatc aggaatccag ttgaatgtca
    10861 gttccgtatc ggttttttct tcgatcaagt tcacaatcgt gtctgtaggc ggtgaagcat
    10921 gatgcaaaat atttaaccaa gtgattttag cttttccatc ttctgtctct gaagaactgt
    10981 tacttgaact gctgccgcat gcggcaagtg ctagaattcc tgcaaaacta agacctaatt
    11041 ttacgagcgt tgcttttttc ataaattttc tccttcactg ttttaataat atttttgttt
    11101 tcttctgttt tttctgtata tgagttcttc cattccaaat ggcaaaatat agaatttgct
    11161 gtccacgtga ctgcggaaaa gaagagactg ccaccgataa aggttaaaac acctggcatt
    11221 gtttggatga tcatcagata aatccctacc atacttaata tcaacactaa tgagtaatga
    11281 agatgtgaga atccgaataa aaatgccatt ttcattttga aaaaatatcc tttccaatca
    11341 taatgagcca tgattgggaa aatatagagt gctgagagca catatatgaa cgcagcaata
    11401 tacaaagcga tcctaagata ccaattgtgt atgaaagaaa gatccacaag taaaacaatc
    11461 ccgatggcgc catatatcca gctcatcagc aaggtttcac gatagttttc tttaaaatat
    11521 gtgaagtacg ttttcgtcaa aggaaacgtt tcttttgagc gcagccattt tctaatcact
    11581 gcaaccatcg catacgatgc aggaccgaaa gtaaaaactc ccaaacctgc gatgactaaa
    11641 ccgccccaca gaaaattcaa ataaaccaac tgtaaaatac gatttagcct tccgtttatt
    11701 ttatctaatg actgcagcat atctttccct ctctttctat cttagtaaac gccgtcttct
    11761 cctagctttt tggcaatctt attagctaaa attaccataa acaatcctac gaccccttta
    11821 aacaatccca ttgctgtact gaaacttaac tgtccattct tcaatccagc tgtataaata
    11881 taggtgtcga aaatttctgc aacgcttcga ttcaatgaat tcaataacaa gtacatatgc
    11941 tcgaacccta agtctaatgt ctgaccgatt tttagaatca gcaaggtaat gattactgga
    12001 cgaattgccg gcaatgtaac atgccatgtt ttgcgtaagc gtcctgctcc atccatttct
    12061 gcagcttcat acaactgtgt atcgactgca gttatcgctg aaaggtagat gatcgtagac
    12121 cagcccaact ctttccagat cacttgtccg atatatactg tgcgtgtcca ttcaggggat
    12181 gttagaaaac tgattttttc aaatcccatt gttgcaagga attcattcaa tacccctccg
    12241 tcaacattca aaaatacata agtaatcgaa acaatgatta cccaagacat gaagtgaggg
    12301 atgtaaatga tcgtttgaat cccgtttttc aatcgtttgt tcgttacttc atttaatagt
    12361 aaagacaaaa tgattggcat cggaaaaaac acgaagatat ttaatccgaa caagatcaac
    12421 gtatttctta ataatgtaaa gaatgtcggt tctgtaaaca aacgaatgaa atgtttgaaa
    12481 ccgacccaag gacttcccat tattcctaaa aatggttgat aatcttgaaa agcaataacg
    12541 agtcccccca taggaagata cctaaaaatc aagaaataaa ggactcccgg cagaatcatc
    12601 acatataaaa atttattcgt tttgattcct gcaattattt tttttcgtct tctgagcttt
    12661 tcttcttttt tcgatagcgt cagttgaaat ggttcttgtt tctttacact ttccataagc
    12721 ccctccttag ttttgtgaat gtttgctgct tatgacttca tgatagataa ctatctgaat
    12781 tacgtatata atcattttta ccgtaagtaa aaacgcttac atagcaatga tttttgtgct
    12841 atacaaaacg ataaagtgta ttttttcaca aataaactac gcatagcaaa ccttctattt
    12901 ttttctatct gacacttagc ataggaaatg attatatacg taatctttca ccgatgataa
    12961 attgaacata tagaaacaga aacatttaat ttattttgag gaggagccta tctatatgaa
    13021 aaagaaagtc tctataggag aaagaagttt tacacttttc aacaatacgt ttctaatttt
    13081 gctagctttg atctgtatcg ttcctttttt aaatatcatc gctacatctt ttgcttctac
    13141 acaagaagtc gtcgcgaaaa agtttatcct ttttcctact accttttctt tagacgcgta
    13201 tcgctacatt ctttctacgc cgaccatttt tagagcactt gctgtttcga ttggtgttac
    13261 aggtgtcgga acgatcgtca gtatgtgtgc aacttcactg atggcctacg gtctttccag
    13321 aaagtatttg tttggtcgag gtttcgtcaa ctttcttgtt gtattttcta tgttgttcag
    13381 cggggggatg attccaacgt tcttagttgt tcgttctcta gggctggtca attcttactg
    13441 ggccatgatc ctgcctgtgg ctgttaatgc aatgaatatg atcattatga gaaacttttt
    13501 ccaagccttg ccggatagtt tggaagaatc agcaaaaatg gatggttgca cagattttgg
    13561 tgtatttttc aaaattatgc tgccattagc gttaccgtct attgctacta tctcgctttt
    13621 ttatgctgtt acttactgga acacttatat gactgctatt ttgtatatca acgattcatc
    13681 taaatggccg atacagattc ttttacgcca aatcgtcatt gtatcaagtg ggatgcaagc
    13741 agaaagtagt gccgttgatg tgattccacc agcacaaaca gttaaaatgg ctgttatcgt
    13801 tattgcaaca gtgcctatgc tgattgttta tccttttgtt cagaaatatt ttgtcaaagg
    13861 agcactcgta ggttctgtca aagggtgatt atgtactgaa cgcaaaatgg tagttgtgtc
    13921 agaagtggtc agcttcaaga aataagaagg catttacgaa aattgcttct taaatttttg
    13981 tgaaatttcg gcttattttt cgaagaatct actcctggaa caccgtgtat cagaggttgt
    14041 gaaagagacg ttcagccttg agcgatgata aaaactctca caaacattgc gacgagtaac
    14101 ccacaggagc aataagaagc gattttgaaa aatagcttcc catatttcac aaaattatgc
    14161 cttattgtcg atagcctgtc tcttgaacgc catttattcg taaaaaagaa ctgtgacaag
    14221 acttttgtca cagttctttt tgcgtgctta gccttgaaat ttaactatcc ataaatacaa
    14281 atactagggt tattctaact tcttttattg cttgaatcag tccacaagaa aaacagcctc
    14341 ttgattcaca aatatttttt tcgatattct cccggcgtaa tcgattcctt ctttttaaag
    14401 aaacgaataa aattttgcgg atttcgatat tgcaatcttt ctgcaatctc ttttacggag
    14461 aggttagtct ctttcaacca tttttttgct acctctaaac gatgattttg aacaaaatca
    14521 ccaaattttt ctcctgtttc ctttttaaat acattactta aatagttagg gttataatgc
    14581 aacctatcag cgatcacatc taaagacaga tcacgatcat attctgtgta gacgatatgc
    14641 ataatattct ccgacaaatt tttaaattca tgatcagtga ttgcttgaga gcattctgtg
    14701 attggcagaa ctaattgttc taaaacaagt ttttctagtt ctttgggatg ataattgttc
    14761 agtgctttta cataaatttg tttcatgtta tcaaagtttt ttgaatctac tcccaataac
    14821 tgtcctaatt gaacgagttc attgatcaag cggaccgaaa ctacttcccg actcaaagga
    14881 tttttatttt gtgcaaacaa tgcatcaatc aattcatgta ctaaattctt cacttcctga
    14941 tttccagaac gaatggcttc aaacaatcga ttttgttctt ctatcggata tctaataagt
    15001 gttttttcat gagcagcagg aacgatttcc tgataaaaaa taattgaact tggaccagta
    15061 tttacacgat aatacagggc ttcttttgcc cgatcaaccg attgcttgct ttcattcagc
    15121 gtttcgaacc ggttactgat gcctatactg atagttaaat tcaagtattc ttttacacgt
    15181 ttttgaatga gtcggcaata ctccatgact tttttgcttg cttctgcatc atctttatcc
    15241 attcgataaa tagtggcttg catttcttca ttcaaaacga tcggcagcat tcgattttcc
    15301 tttggcacaa tttccgtgat catgttgttg atcgctaaaa gtagcaaatc acgctcccca
    15361 ccgtgactat cttctaacag atcgatttga accaaagctg tataatagca gtcattttga
    15421 gaactataac caaattgctg caaccgctga tctagttctc gcctttctac tcgatttcta
    15481 aataaactaa gtacaaatag cgtttccagc tgtggctttt gggtaactaa gtgtgcactt
    15541 aacgcttctt tttctcctat gatccgactg actgattgag cgaccaaatc tagttctttc
    15601 ccactgaatc cttttgattt aagattcaga cgttcctgta tttgagaaat cggacgggca
    15661 aatctgtccg aaaataagta ggaaagaaga ccaatgagtc ctaccagtgt taaagacact
    15721 gcaactagcc cgattctcag attgcggatc attgcgccaa ttgcactaga gtcaacttct
    15781 aatctgtaaa tccaattatt ataatctgat ttcagtacta tatacgtttt atctttttct
    15841 cttatcactt tcagctgatt gtaatctaga tccacgtccg ttaatacagt tggttgctct
    15901 ttatttttta ctgtctgagt agtaaaaagg ttgccaactt cgttttcaat agaaagtatg
    15961 tcttctccac tttcaccaac aacttgttct attgtatgtt tctctatcgt agcaattccc
    16021 aatgcatatt tttctttttc aaacattggc aaagaaatca ccatttcgat tcctttgttt
    16081 ttcttttgcc aaaataaatt atttttgtta tttatgtact tctgttgata cttttcaact
    16141 tcctcttcgg tcagttgact caacgatcca ttgattactc cccatttacc agccaaactg
    16201 actaaatcat aattattatc atccataaca atcgtttcta taaaatttag ttctttcctg
    16261 atttcagaat aagtggagta atctgtatat gtcagcggat gcttgaatat gtccgagaaa
    16321 tttttggtcg aaccatacgt agaaaacgaa tattcaatcg ttctaatttt ccattcaacg
    16381 ttagttttaa cttgcctgag caaattcttt ttgtctgtta aataactcga ttcgatcgat
    16441 tcataggtat tctggtaaat gaataaacta aatcctatga caagtacggc tcctaataaa
    16501 aacatcggta aaaatatttt ataaaatact gtacttttct tcattttctt cctctcctcc
    16561 ttttaaaata aaaggctttc atttttctgt aaaattgata ctcatctaat taaatgagca
    16621 ttccccgctt tcaagaatag cataattaaa aataaaaatc caagtaattt ttaacgtgtg
    16681 caatcttttc atatcaaaga tctattgact tatcgtagtt atcgtaatat gatgaatgcg
    16741 ttaacaaaaa gtgaaaaagt aaggaaaatg atggaatggt aacgatcaaa gatatcgcta
    16801 aagcagctgg cgtttctcat acgacagttt ctcgtgcttt gaataacagc agtttaataa
    16861 aaaatgaaac aaaacaaaaa atacgacaac tagctaagga aatgaattat tcgccaaact
    16921 atagtgcaaa aggtttagtg aatcaaaaaa aatatctaat cggactattt ttctcaagtt
    16981 tgcataaagg aacttcatca aatttcctag cagattccat tgcaggcatc cattcagtat
    17041 tagacacaaa ttacagttta tctgttgaaa gtatcgatgt tgttgattta acagaaatca
    17101 acctgcaacg ctacgatgga ctgatcgtta tgagtcaaag tgatgatgat cagcctttca
    17161 tcgactatct caaagaaaaa gaatttcctt tcgttgttgt caatcgtcac atcaatgatc
    17221 cagcggttat caacgtcact gccgatgatg cggccggtgt tatgcaggca gtagattacg
    17281 gtattggatg cggacatacg aagatcgctt atattggcgg aaagaaaaat tttcgttcga
    17341 caaaagaacg ggaaaaaggg gtattgaaaa gtctaaaaaa agcgagcatc cctgtcaatc
    17401 cagcctattt tttaacagga gattacagct tagaaagcgg cctccaaaac atggaatacc
    17461 tattatcact ttctgacctg cctactctgg ttttttgcgg caacgacgac atagcgattg
    17521 gtgcattgag agctgtacat ttagctggcc tccgtgttcc ccagaacatt tctctgatcg
    17581 ggttcgatga ttctccagtc gtctcctatc tcaatccgcc gcttaccacc gtacgtaagc
    17641 ctttgaaaga aatgtgtcaa gaaggaacaa agcttttact agatttgatc gaaggcagga
    17701 cgatcgaaca atctaaaatt gagctctcca ccttgttgat ccttcgtgag tcgatcaatt
    17761 acttgtaaac tacaagtaat tttatataaa aatgttaacg tgtgcataac acagaaagga
    17821 agtttatatg aaaaaactga caaaagaatt agtgaaaaaa aacgaagcac ctgtaaaagt
    17881 tcttcagttt ggtgaaggca attttatgcg tggatttatt gattggcaga ttcagcagct
    17941 gaataaccaa caattgttta aaggtaatgt tgcaatcatt caacctttag aacaaggtct
    18001 tggaaatatg atgaaccagc aagataattt atatacggtc attttacaag gactcagcaa
    18061 tggcgaaatc gttgatactt ccgagattat caccgtagta gaacaaatga tcaatcctta
    18121 tgatgaatgg gatgcttatc tatctctagc agaaaatgac gacttggcct atatcgtctc
    18181 caatacaaca gaggcaggaa tccaattcgt ggagactgat aatttaacag atactccccc
    18241 acagagcttc ccgggcaaac tgacagcctt tttataccga cgtttcaaat taaacaaagc
    18301 tggctttacc atcattcctt gcgaactgat tgacagaaac ggcgaacgtc taaaagaaat
    18361 cattttacgc tacgcagaaa aatgggattt agaacctgct tttgttgagt ggttagaaaa
    18421 agaaaatgtt ttctgttgta gtttagttga tcgcatcgtc ccaggttatc ctcgcgaaga
    18481 tgcaggtgtt ttcaatcaac ggcataacta tgaagatcaa ttgatggtca aagcagaacc
    18541 attcatgctt tgggtgatcg aagggcctaa agaattggag aatcaactac cattcaaaca
    18601 ggctggcttg aatgtcatca tcacagatga tatgactcct tatcgtgaac gtaaggtgca
    18661 tttgcttaat ggaccgcata cagcgatggt ccctcttgct ttgttggcgg aattggaaac
    18721 tgtagaagaa gtaatgaaag atcctttatt ctccgcatat gttgatcagt tattcaatct
    18781 agaattgatt cctatgcttt ctttacctaa agatgaatta gccatttatg ctgaccaaat
    18841 caaagaacgt ttcttgaatc cttttgccca tcacaaatta gaagcgattt cattaaatag
    18901 tgtttcaaag ttcagcaccc gtctgcttcc agtgttcaag aagtatatcg aagaacaaaa
    18961 tcaggtacca ccgttaatca ctgtttcact agctgcgcta cttcttatgt atcgaggcga
    19021 tcaagtaaag ccacatgatg atgaaaagac gataagcgaa tttactgatg cttggtctga
    19081 taatggaaca gctattcctc gattattgca aaacgctgcg ctttggggag aagatttgtc
    19141 tcaaataccg aatgtgacag atacagttca agaaatagct gcaaagatcg atcaaaaagg
    19201 tgtccggtca gtattacaag aaatgaaagg aacgatggtc catgcttgaa accctgcaac
    19261 caacaatgaa attaaatcga cgagacagtg tggtcgtcgc attaacccct attccaaaac
    19321 aaacacaact gacgattgat cagcaaacga tcaccactct tgaagatatc ccgcaaggtc
    19381 ataagattgc attgggtgat ttgaaagctg gagacaacgt gatcaaatat ggctatccaa
    19441 tcggtcatgt gacgacagac gtaactgccg gtcaatggct gcacactcat aatgtaaaaa
    19501 ccaatctttc tggtgaattg gattatcgat atgaaaaaga tgtccatcca agtcattacc
    19561 tttttgagaa ccgaacattt caaggttact tgcgaaaaaa tggaaaagta gggattcgta
    19621 atgatttgtt tttagtacct accgttgggt gtgtgaatgg cattgctgaa ctgatcgtca
    19681 aacagttcaa ggaaaaacat ccagatctgg gacagtttga ccatattacg attttaaagc
    19741 atccttatgg ctgttcgcag cttggaaaag atcatcaaaa tactcgagaa attttagcgg
    19801 acgcagtaaa tcatcccaat gccggaggag ttttagtatt tggattaggt tgtgagaaca
    19861 atacagttcc tgaattcaaa aaaattttag gtgaatatga tgaagaacga gtgaaatttc
    19921 tagtggctca agatgtttac gatgagatcg aacaaggtgt ggctctatta gaagagcttt
    19981 taacagcggc ggaaaatgac cagcgcatat cagtcccgct ctctaagttg aatgtcggtt
    20041 tgaaatgcgg cggttctgat ggtctatcgg gaataactgc taatccttta cttggtgcat
    20101 tttctgacta cttgattgct caaggaggca gcacgatctt aacagaagtg ccagaaatgt
    20161 ttggcgcaga acaagtattg atggcacgag cggaaaatga agaagttttt gactcaatcg
    20221 ttgacttgat caatgacttc aaacagtatt tcttgtctta cggggaacca gtttacgaaa
    20281 acccatctcc tggtaataaa gcaggtggga tcactacatt agaagataag tctttaggct
    20341 gcacgcaaaa atcaggaagc tctcctgtcg ttgatgtgct acaatatggc gaaaaaatca
    20401 gaaaaccagg gcttagttta ttgcaagctc ccgggaatga tcttgtagct gcctctgctc
    20461 ttgcttcatc cgattgccag ttggtcttgt ttaccacagg aagagggact ccgtttggct
    20521 catatgtccc aacggtcaag gtatctacga atacaactct ttttgaacgc aaaggtcatt
    20581 ggatggattt taatgcaggc gtcttattag aacaaccaat ggaagtaatc ttagaatcgt
    20641 ttgttcaaaa aatcatcgct gtagctagtg gagaagaaac gaaaaatgaa caaaatgacg
    20701 ttcgagaaat tgccattttt aaaaacggag taacactttg attttcataa aacagtataa
    20761 aaagaaagaa ggaagattta tgtttttatc tgatgatttt ttattaaaag acgattgggc
    20821 gaaaaagctt taccattcct acgcaaaaaa catgcccatt attgattatc actgtcactt
    20881 agatccaaaa gaaatttacg aaaacaacaa tttcagcaat ctgacagaag cttggttagg
    20941 aggcgatcat tacaaatggc gcttgatgcg agcttgcggc gtgcctgaag aaaaaatcac
    21001 aggaaatgct tctgattttg aaaaattttt agcttggtgt caaaccgtac caaaaattat
    21061 cggaaatccg ctttacagtt ggtcacatct tgaattgaag cgtttcttcg gtattgactt
    21121 gttgataaat gaagaaaatg cagagaaaat ttggacacaa gcaaatgaaa ttttggcagc
    21181 tcctgatttt cgccgtcggg aaatcgcaaa aaaatcgaat gtagaagtga tttgtacaac
    21241 agatgatccc gcagacgact tgcactatca tcagttgctt gctcatgaag aaccagattt
    21301 gaaggttcta ccttcattcc gtccagataa agcactcaat attgaaaaag aaggatttaa
    21361 tgcatgggtc acactactgg aagaagcagc tgaccaaacg atcaccacct atcaagaact
    21421 gatagaagtc ttagggcagc ggatagaata cttccatcaa catggatgtc gtgtttccga
    21481 ccacgcttta gacatcttac gctataaaga agcggatgaa acagtattag aagagatttt
    21541 tcaaaaagca cgagctaaca aactattatc ttcagatgaa atcgatgctt atcgtacaga
    21601 aacactgata catttgaccc acttctatca ttctcataat tggacgatgc agctgcacat
    21661 ccatgcttat cgtaactgta atacacaaat gttggaaaaa ttagggcccg ataccggcta
    21721 tgatggaatg aatgatcttt ctttgactat tcctctgcaa aaattattga accgtgcaga
    21781 agaaacggat cagttgccaa agacgatcct ttattcattg aatccgaatg actatcctgc
    21841 gattttagct ttgatgggct gtttccaaaa agaacagaat ggaaaactgc agttaggttc
    21901 tggatggtgg tacaacgata ctcgagctgg tatgcgcgat caattgacac aatttgctga
    21961 tggtagtgct ttggggaatt ttgtcggcat gctgacagat tcaagaagtt tcctgtccta
    22021 cactagacat gaatatttcc gtcgtgttct ttgtgaattt ttaggcgaaa tggtcatgcg
    22081 aggagaagca cctgaagatg aacaactttt aggaagtcta gttcaagcga ttagttatac
    22141 aaatgctaaa aattactttg gcttttttgc ctaattgatt aatagtaaca gtaacgaatc
    22201 aaaaacagga tagtgaaata acggaatccg tttatccacg ttttagagaa gaagacggac
    22261 caaatttgct ccgtcttctt ctcttgctgt tctgcttcat tgcctataaa cctttatgaa
    22321 agaagagata acatgcctac tatccatatt gccggtgatt ctactgcggc agaaaaagat
    22381 gaaaacaagc gtcccgagac aggttgggga gaaaaaatct ctgcttattt ttcacccgag
    22441 atccagatca ataaccaagc aaagaatgga cgcagcacca aatcatttat ccaggaagga
    22501 aggttagccc gtctagcttc acagtttcag ccgaatgatt tcttatttgt acaatttgga
    22561 cataacgacc agaaaatcca agaagaaaga ggtacgcttc ccttcggtag ctatttaaaa
    22621 aatctgcaga tattttttga atgcgcaaaa aaagcgaacg tccaaatggt cttactgaca
    22681 tctgtgacgc gacgagatta cttagaaaac ggaacattga atccagatat acttggagac
    22741 tatccaaaag cgatgcgtgc atttgctgag aaacaccata ttcctctttt agatgtgttc
    22801 tcaagatcac aagaattgtt ccagaccttc tcaaaagaag agacaaaaaa attctacctg
    22861 catcttatac ctgatacttg taaaaattac ccagaaggac taaaagacaa tacccacttt
    22921 agtccagaag gcgctgataa agttgcccga ataattgttg aattgataaa agaatcccgt
    22981 ctgcctcttg ctaattattt acaaaaagga gtttgagatg tttccattaa atttaggcat
    23041 ccgtgcccac gatttagatg ttcgaaatcg tgaagaaatc gttcaaaaag tatcacagct
    23101 tcacttgtcc catatccaat ttgcaccaca aaaagcattt cctgaacaac ttaaattaac
    23161 tgacatgaca cttgggagtg ctgcctattt tggtgaatac ttccaaaaaa acaatatcga
    23221 actttccatt ttaggatgct atatcaacct atccagcaaa aatcaagaag ttcgtgcaca
    23281 agcattggca actttcaaac agcaaattgc tctgtgctct gcctatcaag ccaagatgat
    23341 tgctacggaa accggtagtg tctctgtcgg ttacacaaaa gaaaatttta ctgaagaagc
    23401 ttatcagatc gccagaaatt ctatcattga attagtcgct catgcagaaa attttggcgt
    23461 aactgttgct gttgaagcag ggatcaatca tccaatctat aactatcagt tagctaaacg
    23521 tttaatcgat gaagtagctt caccaaattt aaaaatcatt ttagattgtg cgaatttgat
    23581 gcataaagaa aatcatgctg atcaggaaaa agtgatccga ggtgccttgg acaatttaca
    23641 tggttatatt acagcgcttc atctcaaaga ctatatcatg gaaaatggaa agatccgcat
    23701 ggtacctgtt ggcaagggct gtttagattt ccgcccggta ctgcattata tcaaatatga
    23761 gcgtcctcta atgtacgcca ctcttgaagc tactcctgag atttatgttc ctgaagcaat
    23821 agaacacttg cagtttattt accaagattg ttaaggagaa gattatggag gaacgaatac
    23881 agaaaagtta tcaaaaatat catgcaaatg cttttcagac attgctgcat ctttatcagg
    23941 gaaattattg gcgttttgcc atgtcaggac tcttttttat tatcaaacat cttccatctt
    24001 ggttaatgcc gatcgctatt gccaatgtaa tcaatgcagc ttcatcgaaa gattcttctc
    24061 agttgcaggc catttatttc aacgcttttt tgatggcttg tctgattggt caaaatatat
    24121 tgacgaatta ttttcatgta aaataccata gcacttcgat ccgtcaagta gaagctggac
    24181 taagagcgac ccttatcaag aaaatccaag aactatctat tccttatcaa aaagaattgc
    24241 aatctggacg aatccagtca aagatcatcc gtgatgtaga agctatccaa acgttgtcct
    24301 ctcaagtatt tgtcagcgga ttaaatatcg ctgtcaattt attcgtagct ttaggtatca
    24361 cgctttataa aagtcctgtt gttttcatgt ttttcttatt gactgttcct ctcgccgcat
    24421 tcgttactct ctttttccgt aaaaggatca gacagtccaa ctcagacttc aggcaagaaa
    24481 tggaagagac ttctgcaaaa gtgatcgaag tgatcgaatt agcaccgatt gctcgagctc
    24541 atgggttaga aaaacacgaa acaactcgct taaataagca ttttactcaa atctatgaaa
    24601 aaggattcca cttagatatc ctgcaatctg ttttcggatc agtcagctgg gctagttttc
    24661 agatatttca gctgatttgt ttgattttca ctgggatatt agctttacaa gggcgcatcc
    24721 aacccggcga tgtcgtgatg tatcagacgt atttcacgac gatcgtcaat tccgtttctg
    24781 gaatgatcac gctgattcct acgatttcaa aaggcatgga atctatcact tctattggcg
    24841 aactgttgat tgctggcgat gtagaaactt atcacggtaa gaaaaaaatc caacatttgg
    24901 atggaaattt tcgttttgag gatgtttctt ttctttatcc ggactcactc aaaccgatta
    24961 tcgatcattt ttctctagag gtgaaagctg gagaaacaat tgcttttgtt gggccatctg
    25021 gctctggaaa atctacactc cttaacttat tgattggatt tatccagccg acttcaggaa
    25081 aaatttttct agatgatcag cctttggatg aactggatat gcgaagtgtg cgaaattatt
    25141 tagccgttgt tcctcaaacc actttgttgt tctccgcttc gatcaaagag aacatcactt
    25201 atggcttgaa aaacgtatca aaagaacgtt tagatgaagt catcgaagct gcgcaacttt
    25261 cttcactgat tgaccagctt cctgatggtc tggatacctt agtaggcgaa catggcaaca
    25321 agctgtctgg tggccaaaag caacgtatct ccattgcacg agcgttgata cgtcagccaa
    25381 aaatcatttt gctagatgaa gcaacctcag ctttagataa ccagtcagag aaaaaaaacc
    25441 agcaagccct gaattacctg acagaaacac ccactacttt tattgtggct catcggcttt
    25501 caacgatcaa agaggcagat aaaatcgttg tgatcgatga aggaaaaatc gttgaaatag
    25561 gtacttacga aaaactgtta gctcaaaaag gctatttcta ccgcttgaaa aatgaatgac
    25621 aaaaaatagg gctctataat ttatgggact gatgaatcag aattg
//
LOCUS       DAZUMK010000031        23840 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000031 DAZUMK010000000
VERSION     DAZUMK010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.031"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..229)
                     /locus_tag="ACG808_001211"
     CDS             complement(<1..229)
                     /locus_tag="ACG808_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046851.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNE"
     gene            complement(236..784)
                     /locus_tag="ACG808_001212"
     CDS             complement(236..784)
                     /locus_tag="ACG808_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFS5046852.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            complement(829..1161)
                     /locus_tag="ACG808_001213"
     CDS             complement(829..1161)
                     /locus_tag="ACG808_001213"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046853.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(1175..1432)
                     /locus_tag="ACG808_001214"
     CDS             complement(1175..1432)
                     /locus_tag="ACG808_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046854.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(1627..2169)
                     /locus_tag="ACG808_001215"
     CDS             complement(1627..2169)
                     /locus_tag="ACG808_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFS5046855.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            complement(2190..3281)
                     /locus_tag="ACG808_001216"
     CDS             complement(2190..3281)
                     /locus_tag="ACG808_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS5046856.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(3289..5499)
                     /locus_tag="ACG808_001217"
     CDS             complement(3289..5499)
                     /locus_tag="ACG808_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFS5046857.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(5515..8043)
                     /locus_tag="ACG808_001218"
     CDS             complement(5515..8043)
                     /locus_tag="ACG808_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5046858.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAVSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(8095..8505)
                     /locus_tag="ACG808_001219"
     CDS             complement(8095..8505)
                     /locus_tag="ACG808_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFS5046859.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(8518..8736)
                     /locus_tag="ACG808_001220"
     CDS             complement(8518..8736)
                     /locus_tag="ACG808_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpD family membrane protein"
                     /protein_id="HFS5046860.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(8733..9725)
                     /locus_tag="ACG808_001221"
     CDS             complement(8733..9725)
                     /locus_tag="ACG808_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS5046861.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(9804..10715)
                     /locus_tag="ACG808_001222"
     CDS             complement(9804..10715)
                     /locus_tag="ACG808_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFS5046862.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(10734..11078)
                     /locus_tag="ACG808_001223"
     CDS             complement(10734..11078)
                     /locus_tag="ACG808_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046863.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(11098..11376)
                     /locus_tag="ACG808_001224"
     CDS             complement(11098..11376)
                     /locus_tag="ACG808_001224"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046864.1"
                     /translation="MSVEHFCDCYIALLRSQQLLTVERYGAEFGYVLQAERHRATRCY
                     EKEDQVIDGYEELCIRNNRYLQQRLYEQLMMDFSNLYRGYECSMIQER"
     gene            complement(11523..11654)
                     /locus_tag="ACG808_001225"
     CDS             complement(11523..11654)
                     /locus_tag="ACG808_001225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046865.1"
                     /translation="MSLEFLGEPIEKEEFVSHYMDLFIQVIKNFVRLMNFIHANKTI"
     gene            complement(11735..12235)
                     /locus_tag="ACG808_001226"
     CDS             complement(11735..12235)
                     /locus_tag="ACG808_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046866.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(12236..12781)
                     /locus_tag="ACG808_001227"
     CDS             complement(12236..12781)
                     /locus_tag="ACG808_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046867.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(12778..14034)
                     /locus_tag="ACG808_001228"
     CDS             complement(12778..14034)
                     /locus_tag="ACG808_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFS5046868.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(14395..14706)
                     /locus_tag="ACG808_001229"
     CDS             complement(14395..14706)
                     /locus_tag="ACG808_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046869.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(14729..16204)
                     /locus_tag="ACG808_001230"
     CDS             complement(14729..16204)
                     /locus_tag="ACG808_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFS5046870.1"
                     /translation="MKAHFFKYRGRRIRYFNRYLWFCYYGFFLFPFLILLGWFSWKVF
                     YPRLSTFSNWQMYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLVRML
                     IDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGHLLS
                     EVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPNMLI
                     AGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEEMII
                     CLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDMEARN
                     RVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYLMTF
                     GEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYDFTV
                     KEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(16653..17105)
                     /locus_tag="ACG808_001231"
     CDS             complement(16653..17105)
                     /locus_tag="ACG808_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046871.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(17107..17472)
                     /locus_tag="ACG808_001232"
     CDS             complement(17107..17472)
                     /locus_tag="ACG808_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046872.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            complement(17571..21854)
                     /locus_tag="ACG808_001233"
     CDS             complement(17571..21854)
                     /locus_tag="ACG808_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HFS5046873.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            complement(22001..22240)
                     /locus_tag="ACG808_001234"
     CDS             complement(22001..22240)
                     /locus_tag="ACG808_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046874.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(22330..22572)
                     /locus_tag="ACG808_001235"
                     /pseudo
     CDS             complement(22330..22572)
                     /locus_tag="ACG808_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(22685..22921)
                     /locus_tag="ACG808_001236"
     CDS             complement(22685..22921)
                     /locus_tag="ACG808_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046875.1"
                     /translation="MFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTELKDKN
                     NVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
BASE COUNT         7023 a         5159 c         3487 g         8171 t
ORIGIN      
        1 cttcattttt acttgttatt ttttcattga ctataactag ttcatctggt aaaaaaatta
       61 accaacaaac atcgacaaac agtaaatgat ataatccatt tccacctcca agcatattca
      121 atactggtga acatgcctta gaaacacgaa tatagttatg gtttaaatag atattttctt
      181 taaacattgc ttgtttaaaa gccatatgct cttgttctaa acttttcata aacttttact
      241 ctaatgcaca gctagcagca gctgctgcaa ttcctgctac aatatttacc ttagctaact
      301 ctttagctgc agcttttcct aattgttttt ctgcaatttt taataataaa tccgcagctt
      361 tttcaaaatt aaaagctttt aatgcttcaa taaaatctcc ctgcgtacct atagcccaag
      421 ccactcctcc tccaggaaga gaattaatgg ctacacatcc agcatatcgt ttccaatcaa
      481 ctttctccat tctaggaaca ataatatgtt cataaaagac tcttgctgct cttccttgtt
      541 cgttatcttg actgattcgt tcacgaatag caccaaaatc ctttatatgg tatacaccat
      601 gttcgtctaa aaaaccaact gtatcaaaat aaaaagttaa ttcatctact aatttattct
      661 ttatttctaa atcaactgta ttcacttttt ctgtggcata cacacttgtt ccagtactaa
      721 caaaagtact taaacctaat aagaaaacac ctaaaacacc aaatatttta attattcttt
      781 tcataatttc tcctccataa aaaataagtt actttttaaa tatcttgttt atgaattcat
      841 tccccaaagg ccttttaaga ttgatacagc agtccgttca cttcttttag acttgtcata
      901 caagtctttt aaatgatccc atatcagact agtttgtcta tctagcttca atccattaaa
      961 cgctaatgaa cggttcattt tttctattac tttagataaa gctacagaaa tttttatacc
     1021 ttcacaacgc gcatcttgga tttcaataat agtatcatca attagttttt gaatttcttg
     1081 cctatcaaca tattcaatct tagaaagaga atcttttaaa gctactaatg attcaacgat
     1141 ttcttttttc tcttcagaca ttttcttcct ccttctagtg acccgtaaag ccatgtagag
     1201 cagagtccat tgaaccagta attacactat ctacaacgcc attccaattt actctacatt
     1261 ttccattttt cagatttgtc ggtccatacg gatagccttt ttgacattca atgttcggat
     1321 tataccagat aggattatac cattcagccg cagaagcaga ttcagtcgat acaaacaatc
     1381 ccacaccaac aaatccaata cacatcacac tatagccaat tagttttttc atctcgtatc
     1441 catctcctaa aaaatagaat agttcttata gttattcata agacatattt tttccgtttt
     1501 tgttttatcc tttctgtcct ataacaactt ttttcaagta aagaatgaca tagtttttct
     1561 taaaaaaaca gtctgttttt ttctatttct tcggtgtata ataaatatga gtgttcattt
     1621 tttattttaa tacgcactcc gtacataata aatcttgatc gcatcaatct tttgagcttc
     1681 ttgatctact ttcaattgaa gtaatacaaa ttgagtaacc ttattcattg tttcttctcc
     1741 aaataacaaa tattgatccg gattctctat atcttgtgtc acctggtaaa gtttcccagt
     1801 tgttttcatt tgtacatgcg ggtctacatc aatactattg tctttaatac aacgatctgt
     1861 catatagtcc ctcacagctt gattgcgttt ataaacatct gtgaaattac tccattttct
     1921 agcaaaatct tttaagaaat tttttgtcat ttcatctatt tgcatttgtt gcgctgtctc
     1981 gtcttctaat gaaccaattt gactgttact ttcagtactt tgaagagttc cacttgtttc
     2041 ttttactgct tcgattgttt cttctgtcgt tctcaattgt gttttttgtt tttcagaaga
     2101 aaaccaatga caaccagtaa gacctatcaa acataaccaa ctaaatacta aaattatccc
     2161 ttttttcacg tttctagtca ctgcctttct tatttcacct tacgaaccag actacgaatt
     2221 tttgtttgat tgtacgtgcg ttgataccga gccataatcc gtccttgctc ggcattctgt
     2281 tccacactag agaaatgttg tccgccatat gaaacatctg ttataattcc ggtgtgacca
     2341 tagattccag gagacaatgc accacctgct tgccaattca ccacatctcc tgcttgtaaa
     2401 tcacttggtt ttgggttcat gattacttgc caaccatagg ttgtccagtt ataatcttcc
     2461 cctattaaca tcgcaaacat gtgtccagaa cccattaact gtgggccacc taattgagat
     2521 acataccatg ccgtaccacc ataacattcc ccattattta cggatcgtcc aatgatccga
     2581 tccaaaacct tcgcattgcc tttacttttt cctgcaataa gcgaagtttt ttttgttggt
     2641 aggctattcc aatgcttgat cgcaaaagta tcatattccg ttaagttata ggacgttatg
     2701 atactcattg ctcctcgata atacggactc ccttcggtaa tacctgctgc cgcatagcct
     2761 ccacgaccta gaatcaataa acaacttcca ggatctttat tgcccacaca ttgattaaag
     2821 ttgctttggc tcgccaccat aaagccataa tagtcataac aagcttttaa gttcggaaac
     2881 ttcatataga aaccaccttc agaaccacca attccacgtg cagaaccttg tgggtaggga
     2941 caaccaggaa accaggtaat cccaaaaaag ttcgtatcgt ttcttccaga ttgtgactgt
     3001 ccataagctg attcatagcc taattgagaa agaatcacac tcggtaataa ctgatacttt
     3061 acagctgcac caactgctgc ttgcgcatgt tcatctgtga ttggaccacg tcctttaatc
     3121 tctgaaaaaa tcggtaatcc ttcggtatat tccccattaa aagcgacagc tagacctgta
     3181 gcttctcctc catgctcaga aagttctcct gaaaaaaaga atggcaatag aaaaaaggga
     3241 agaataaaga aaagaagaca acctagacac cctttcttca tttcttcctc atcctttctt
     3301 cgataatggt ttttggactg ttcgtccttt tggtaaagta aaatcagttc ctggttttgc
     3361 gtccaaaatc ggcagtgttt taggatcctt ttctattttt tccgattgct ctgttggctt
     3421 atctaaagaa gaaaatgcag gttgtaacga ttttggtgcg gaaatcgacg gtttactttc
     3481 agacgaacgt tgccccggtt gttgcacaga acgatttcct gatagagtgg ctaccgacga
     3541 attaggaaca gagtattggt tcaataactg ctgcattgct ggataactcc catttttcgc
     3601 ctgatttttc acttggcgaa tctgttgtga aagagaacca gcagctgtta attgtggcac
     3661 gggtactttt ccagaaggca acggaatcga ttgaccaaca aaagcgatac ctttatttaa
     3721 caatggtttt ctttgattat tcccttgttg cttactcaat tgtgccaatg attgtacctg
     3781 ttgttttggt cttactgaat tcatggacga atttggaatc aaaccatttg aaagaccaga
     3841 attccactct gttttcgatt tggtagaaga catttcaggc tctaccccat gataacgaac
     3901 atgacgacca ctataaggca aggcttgatt tgaacgccca cctagttcaa agggattaac
     3961 cacttgcctc ccttttaccg attcttgtgg tgtacgtttt tgttgaaact tttgaaatcc
     4021 atgtatcgct gcagaagcga gtggattcgt tgctcgatac acattccctg ctaatcctgc
     4081 acctttgatt gccaaatttt gtccagcagt tgatagttga ccaggcagtt gtttcatttg
     4141 agacaaagaa tgacttgcta tgtttttcgt gttgtttata gtttgatctg cagctcctaa
     4201 ccctagtttc tttaataaat ctttccgttt tacaatgagt aaaattgata ggagtgcttt
     4261 gacaaacata tttcctaaaa aaccaaaaac agtattcatt ggaattaaat cgtctactat
     4321 gttatagatc aaaaatgcca ccaaaataat cacactgtaa aaagattttt gaaacatcaa
     4381 gcccaaaaag gactttaagg tattcatgag catataatca aatactggaa caaaagaagc
     4441 gagtaatgaa aaaggaatca caagtaataa acacaacacc aacatttgaa agataaaacg
     4501 aatcatgcca atcactacaa gtggtaatcc aatacttaga ttaagcaatg gcaccataga
     4561 accaacgact gctttataaa cagctttagt tggttgaata tagacacgat aagccttctt
     4621 tttccctcta tcttctcctg cagcagtggt cacttttgtc tcaatatcag ataattcttc
     4681 acttgacgct tccttggcta gaaattcttg tggttcaatc tctgcttgat acaattcttc
     4741 taaatttgtt gtcccataat ttaacaatat atacggatca acaacagccg ctttataata
     4801 aagttggcgc actttttgaa tggcttcctt ttgtgtcgtt ggcactaaaa caccattgac
     4861 atcttttgtc gccaattcat ctagactatt tgtagcagaa aaaataaagc cttctacctc
     4921 cacggacaaa tcatttaacc ctttggtaaa agcttctcct ttactaggta cgttactacc
     4981 atagccaaac caaaccattg aaaaaatgac aacgaagaga aatcgaataa acaccatttt
     5041 tgcgtaatgt ttccctctaa atatacgaag agtaaataga tataacacat acaagacgac
     5101 aatccccgca ccaataggat cgtatatttt tgaataaata gtatccgaaa aagtgaaaaa
     5161 ctggtgaatt aactcgttca ttgcagctcc ttcatagagc ttctcaatga caaaatcaag
     5221 gccttgccaa atcaatttac ttaaactaaa gaacatattc gtcatcattt ttagaaaatc
     5281 tgtgccattt gccccaaata acattttgcc taatgaaaat tcactatcag ataaataaga
     5341 agaaaaggta tcaatatgaa atttcccaat ctcatagatc gattgatcca cctgcttttc
     5401 tgccgcatac accaatccac caacaaaaag cagtccgaat ccaatcgtta aaaaaaacaa
     5461 aacgcaccat ttcttttcct tatgctttat ataagccatt gtttcacctc atttctattg
     5521 catgattaac gtatcctgtt cttctacttg agagtgactt gttttttccg ttgtttgtaa
     5581 tgcagcggtc atttcttcaa acaaactgtg tacattgagt tttcctacac gtccataggg
     5641 atctaacatc caacactgtc ctttaatcat gtggctcatt tcagctaagt tcgcttcatt
     5701 tacttccaaa cgtaaatgat tcaaaatctt ttctcgctca ctgtcttcat caaaagcaaa
     5761 gacgacacca aaattccctt tttctttatc tgaacctaag tcaccaataa attgtgtatc
     5821 taacactaat tgattcatct gggaacgccc cacacgtgcc attgctgcaa taatactacg
     5881 tcctgcgttc gaattattaa atacccatgc ttctgtaaaa taaatcgctg ttttcttact
     5941 actatctcgt tttccaaaca tttcacaaaa acgacctaaa ctaatcatca aacacaaaga
     6001 ctttcgatcc gcatctgtat atagttctgg tcggacattg tcatttggta atttcaaatc
     6061 ttggatctct aaaatcgttg ttttggcatt aaagtctaat cccgggtttt ctccataaga
     6121 aaatcctaaa cgtaaaatcg aatcttctac cgtcaagcgt aaaaactcac catattctcg
     6181 aacctctttt tgttccattt gttctaaata gtcgaccaca tgcatcaagc caacttgttg
     6241 tccggcttct ttttgaacta aaacttcttg gattcccttt aataccgccc cttttaaaag
     6301 gtgattggta cctaacggac ttaactcatc aatcatatcc tgggctactt gtttcgcatc
     6361 tactcgggat aaacaaacaa ttggatctaa tacaccataa ttacggggat ttgtagcatc
     6421 taacgtgaca taactcattt tttgcaaatg ttctacaaac aaagggtaat gtttctgata
     6481 atacgggtct tgaatgagtt tcgtaaacca tttgcgcttt tctcctttgg gatccacaaa
     6541 taaactttgt acattcataa acgaactata caaaaagagc aagccaacta aaaaggtctt
     6601 ccctttaccg gtttttccag ttactgctac atgaggcgca tcaaacaacg cacctttgat
     6661 tccttccgca atggctagaa gattcaaaaa gacaaaacga tttgacgcat aaattaattc
     6721 ttctaatggt gttttttctc ctcgagaacc accatcccct aatgtatctt gccgaccaat
     6781 ataccagccg gattccatac ctaattctga tactgctgaa aacaaccatt cgccaactgc
     6841 ttctacagtt gttcggtgct tccaatgtaa ctgccgtccc aatggttgcc ctaataaaca
     6901 tttatagaac aattctcgtt gatcattttt tgcacggtgg atatggataa tcgtatgaag
     6961 ttgttgaata accgttgcac tattttgacg aacttcttca accgtttgac cgtatacaat
     7021 aaaacatgcg atccaatcga cgacaggttg cccattatct actcgttgct ttaaatgttt
     7081 tactaatcct cgaacagtta ataagcgttc actaggttct tcatcatttt gaaccaattc
     7141 cttatgctct gttttgaacc gttccgatag ccaattcagt tttaaaccaa tacctccctg
     7201 tcctttcaag cgtggatagt gaattttcat tcgcaattct gctgggaaag aaagatcctg
     7261 agcaaattga aatagatcac aatttctcat attttcaggt gtttccccaa taggcaacaa
     7321 tgctaaataa ctactcccta actcatcttc taattgaaga aggcctttat gttccttcgg
     7381 tatacgtaca tacgttcctt gattgtcttt cagtacttcc caattatttt gttcttctac
     7441 tttgtgagcc attcctcgaa tatattgcag acgattgaga tacaacagct ctttttttac
     7501 taagggacga atagaaaagc tataaagtaa ttgtgtcact tcttttacat aaggatcact
     7561 ttcagaaaac aaagtttcca aatcatctac ttctaatcca aataagcgtg ctaaacttac
     7621 tgctaaatct aaccagatta gatttattac atcatgaaaa ctctccaccg ttgctttttc
     7681 tttgactttg attcctaaaa taaatgaagg acgatagact tgaccttctc gttccaacaa
     7741 tgtcactgtt tcttttgcat aatacgcacc aattgcttga ctatcgggag aaaatccctt
     7801 tcctaatgtg tcaaacttcg tccgtaaatt cattgttcgt ggatacatca ccaactccac
     7861 atcttcataa tctgctaaaa gcaacagtaa acgagccatc atttctttta tttcctgtgt
     7921 cttttttggt gtgttttttg aaattgttcg tggaaaaaca tgatagtagg cccagataca
     7981 atcctctcca tttaacaaac aatttttctt gaatgctttt actggttctt ttacaagcac
     8041 caatgaattt cacctcccaa taaaaaagga acaaccagtt gattgtccct ttttctaatc
     8101 ctctaaataa acatacttat tcacaaaaag taattcttcc aatgcattgt ttgatacctt
     8161 gtcccagtta taatacacac aatcaccatt catcatacga atcatataac aaaaccaatc
     8221 ccataaataa aaatgcactt tttttccatc aggttttagt tttgccagtg ctaacacaca
     8281 taaatagggg agtcctatat aaaaaacaac tcctgctggt ggaaaaaaag aaatgaccca
     8341 accaataaat gaacgtaaaa ataaatattc aaagattacg accaacaaaa aagttgctgc
     8401 attgactaat gcaactggaa atggaagacg ccaattgctt gtaatctgtt gtacgcgata
     8461 aggttccgca taaacaatcg aataatcata aggttcccaa tccacttcac tatcctccta
     8521 atccataaat aatttttcaa tgattttacc aaacgcatca aatacacgtg ttggatcatt
     8581 taagaagaaa taaactgcgg cacctgctgc gatagatatt aatgcatttc ctacttttga
     8641 tttcataaaa tttcgcaatc ccagataaac aaccaccatt attaaaaaat agccaccttg
     8701 cgttaatata aaatctacaa ttcccttcag tgtcatttcc gttcttctcc tttcctatcc
     8761 aaccaattat gtgttaattt ttcaacataa tactgatttg tttttttcgt aatgacaatc
     8821 gtcatttgtt ctttttgtaa aagtgacgta tcaccatctc gaaagacgac cgttgctttt
     8881 acaataaaag gttttttcgt ttctttataa cgcaatactt tcatctctac ttgatcaact
     8941 ttagctgttt gattgagtcc ttcaggttct ttcatcatgt atgacatatc ttttgcattg
     9001 tttttcgcat acttatctaa gaaatcttcc acaaatcctt caacatcttt cgcttccgac
     9061 gcatcaaccg tttcgtataa tgtgtcatca taagtaatca ttcgttgttt ttctgtattc
     9121 tttggtagtg ccgtaaaata aggataagca ataatacgac agcccttgtt tgaaatagaa
     9181 aagggaagat tgagtacttg tgtgattgtc acacttgttt ctttcttact ctctggattc
     9241 atcgagcgaa tatcataagt tactttataa ctagcagttg gaatggtttc aatttctttt
     9301 aatccaataa acgaagcagc agttaatttt cgaacacctg aaacacttcc tggttcttga
     9361 ataaagtttt gtgcataaaa ctcttttttt aatgtttctt gacgttgttg ttcttgttca
     9421 tgattatcga atgtattcat atacaaagca acaaaacgat tcataaagga ttcaatttca
     9481 ggtgtgtacg ttaattctga ccgactttgt acttctttct tttgtaattg ttggatttca
     9541 tctttcaaaa acgacacttg aaccttcgtt ttcatggatt gaaaaaatgc caagccccct
     9601 gaaaaacaaa gaaataaaac caaacaccaa atccatttca ctgctttttt tgaatggatt
     9661 ttcttttgct tcggtacttt ctctgtataa actttttctt tccgccgtag ttgtcgtttt
     9721 ttcataagct actccttcta gtatgttttt actagtacgt acgattgaca acgataagct
     9781 tgtttttctg aaaaatagtc cattcaatta ccctcatttt ctattccttt tctctttctt
     9841 ttatatccaa caaatccagc aatacttgtc aacactcctc ctagaacaga aagtgcaaca
     9901 tgtatttctt caccagtctt tggtaagact tttgtttttc catctgctgt tttgatttca
     9961 atattgccac ttggaagttt ctttacagga atcgacaaat cctttaccaa attcccttca
    10021 ctatcttgga ttaatggaat accatctttt acaccaacaa ttgtttgatc acctgctact
    10081 tttaatgggg tggttggtgt tggaatatgg gtcaattcct ctacattact agtgacaatc
    10141 ggtacactca ttccttgaga agtattcgtc gtaatttcac cgcttggggt taccgatact
    10201 gtgacttctt ttgctggaac aataggttta ttttcttttg gaattgcttc tgatttttca
    10261 acaggttgtt tcggttcttc tttttgttct tcactggtag acgacatcgt tggctgttcc
    10321 gtacttgacg aatcagtatt cttttcagaa gaactggttg ttcctgattg ttccggagtt
    10381 gtgactatac ttgaatccgt tgttgttgaa ctatgcacac cttcttcttc gatcgaatca
    10441 gtcggtaaag ttgattcagg aaactctggt tctggtgtca ctggctcttt ttcaggtttc
    10501 tctgactgca attgttcagg ttctgctggt ttttccgttg ttcctccatt tagctctgtc
    10561 tcaacatctg gtaatccttc ccctggcaat gtttctacaa tttctgactt aggtgtttct
    10621 acagtttcat ctgcataaac caatgaactc cctaattgaa acgaaaataa tgaaattcct
    10681 agtgttgtta cgactgctaa tgttgttttt ttcatgatta ttcctccaat tttttatgca
    10741 attcgtgttt tgttcataga cgccgcacta atattatctg aacgataaaa gaactcccgt
    10801 ccgttttctt ctaacccaac atatggatta tttttgaata attgaatcaa tagttcactg
    10861 gtgttgtatt ttggatagtt tgcaaaaaaa taatactgtg cttgtcgccc atccgaaaat
    10921 tcaacaagta attcataacg ataccgcttc aatccttttc gttgttttaa aggatctcta
    10981 taccaacttg tccatcgttc cattcgtgga tagagataga cacgatacac atagaacaat
    11041 gccataccac ataccaccaa aaataataat cgccacataa aaattccccc ttttcttcta
    11101 cctttcttga atcatcgaac attcataccc tctatagaga tttgaaaaat ccatcatcaa
    11161 ttgttcatat aaacgttgtt gcaaatagcg attgtttcga atacataatt cttcatagcc
    11221 atcaatcact tgatcttctt tttcatagca tcttgtagct ctatgacgct cagcttgcaa
    11281 gacataacca aactctgcac catatcgttc tactgttaat aactgttgac tacgcaataa
    11341 tgcaatataa caatcacaaa aatgttcaac cgacatttga tttttctcac gtttgtgaaa
    11401 taggtattct ttagaaagca cattattcgt taaacaatct gataaatagc gataatctgg
    11461 cggtcggtta ttttcatctg tgaatttttc ccataataaa ctgattttta ttcgttcagc
    11521 ggctagatag tcttgttcgc gtggataaaa ttcatcaatc gcacaaagtt ttttatcacc
    11581 tgaataaaca aatccatata atgcgaaacg aactcttcct tttcaattgg ctctcctaaa
    11641 aattccagtg acataccatc ttctccttcc taaaaaaaat aaaaaagcag aaaccgattt
    11701 ggcttctact tttctttcct tttatagctc ttttttacgg aagaatccgc aaatgacatt
    11761 ttttcaaaaa atcaatcggc acaagttcac gaaaacttgg agcaattttg gatagaatca
    11821 cttccccatc ttgtaaaata caatcataaa aatcatcagt tacttgatac ttacgcttct
    11881 tacaataatt gataaaatag tagcgatcat tttctatttc cattcgttgt tctactaagc
    11941 gcatacgaac atcatcatag aaataagcca caaatcgccc ttcaagttca tgtttttcaa
    12001 tgggatactc ttctggtaac aatctatttt ttcgtcgttt atacattgct ggatacctta
    12061 gaagaagttc atctaaacgt ctgtcagtat atggtaactg acgaatcaac gattctaatc
    12121 gttctttata cagatagaat aattggcgca agcaatagtg taccgcctct ggattttcta
    12181 atactgttgg atcacgtgac caatacgcac caacataaat cataattttt ttcatttaga
    12241 caatcacacc agcaatacta atgaccgatc cacaataggt agaacaagtg gcatagcgac
    12301 caatttcctc taattctatc tcaatctctt ctaaagaaag atatgtctgt tcttctgttg
    12361 gctggcgact catttcccaa gtgacagtca ccaatgaaat ttcttccata agaggttttt
    12421 cgctgctaag tgctgccaca cagttttcat atatcttcat tttatcgcag ccttttttaa
    12481 ccgctaaaat cgcaggtatt ggcaatgaaa atcgataaaa gcgaatgaat tctagccttc
    12541 ctttccttcg gctaaattcc aactcttcga gttcttgttc gtatccctta cgaacttcta
    12601 ataagcgtgc aatttcttgc cagttctctt tttcctgata acgtccagct tttaccgctt
    12661 gttcaatata aggaactcga aaacgaaaat aagacaaatc aaccatcttt tcatttaatg
    12721 cttcgattgc agcctttaaa tccaacaggc gcagccattc tgttgtcgta tatgcattca
    12781 tgcaattttc cctcctgacg ttcccatcca ttctagatag ttttgttgct cgtcatttag
    12841 ttcggcatta tcaatccctt cttgtacaaa gtctcttcct aaaatacgat ctgcttcttg
    12901 taacaacttt aatgtcggag ctacttgttt caaaaaccag cgttctgaac gatctaatga
    12961 gaatttttct ggctttactg taaaatccaa ttcttccatc gaacgaaata attttgccca
    13021 tcgtttatca acaatccgtg catcactttc cagtctatcc caaacaataa gttttgcatt
    13081 aattaaacca acgccaatct ctcctaaacg tttcggtgaa tcacctaaaa taaatgcttc
    13141 tactgcttgt gttgctttct cattggctaa ccgtaactca tatcgattat aaataccata
    13201 tttcgttaat gcttctaatt ctgaaatttt ttctcggttc gcaatttcaa aacgtttctc
    13261 ataaaaatta aaaaagagag gactctttcg cgatccaaaa tacaaagata atccttcact
    13321 tcgaaaatag ccatcatcta aaaaacaacc gccaatattc tggaatctct taaagtccat
    13381 ttctaataaa cctgcttttt gtttttctag taattcaaag agatcgaaat gttcttccgt
    13441 ttccaatccc caaagttcat ctaacgcaat atcgaatcgg ctacattgca cacgtgtata
    13501 accagcttcc aactttcctt gatttttcca aatggaatat agccatgttc gccaatctgt
    13561 ttgttgttca cgcatgactt cctcatattc ccggcaacct tgtcctgaaa attgcacaag
    13621 tacattttct gttacagttc gttttgaatg ccagaacacc cagatattcc cacgaacaga
    13681 aagacagtca aaaagataca agcccgttgg acgtgtttca aattctgtca gatccattcg
    13741 taatacacgt tgaatcactg ttttgtagtc tgtcccaaaa aaggaaagac gcaaataatc
    13801 aatcattgct cgaagatcta ttccgctttt catatacaat cgaaagcgtt ccttgattcg
    13861 ttgttcaaaa tcctctggga tttttctttt cccttgttcc atctttctaa tgtaatcttt
    13921 tgagcagccc atcaattgcg caaatcttgc ttgtgatagc ggctttttat ctccacttat
    13981 attatgaatt gcacttaact tctttctgta gctttttagc tcaatcggtg tcattttctc
    14041 gctcctttta taaaataaca aaatcgattt tttttaaccc tttagaaaca ccgatatatc
    14101 aactagtccc cctcgttttt tgggaaaagt agtacaactg ctgaccttag ataatcactt
    14161 gatacaaaag ggtttataaa acttttttct atgtgttatg ttatttctgc cccccatatt
    14221 atatatcggg gggctttaca caaagaggcc gtgcgcaagc gcacgacctc tttgtgagct
    14281 gcacacgctg ctagcgagtg cgcattatag gcccatgctt cgcagcctta atgcgcaccg
    14341 ccaggcagag tgcggctacc agactacgtc tagagccctc ccgatagata ataactacaa
    14401 aattaaatga tctaattttg ttatttcact atatttttca ggagaaaaaa cgcgataaat
    14461 tgacaaagtt aaatcctctt ctaaatctac tcgataatga tacaatcgag tcaatcgttc
    14521 tatatctttt ttcgttagca atacttgatt aataactttt cctgtaaaaa cacgttcttt
    14581 gcgcaaaaag cggtaatcag ttacttgttc aatacgattc aaccaatcat aaaacgttct
    14641 ggaagaaaga gaaggattaa tcattcgtat tgctgtagct actctataat aacaactctc
    14701 tgccatttaa cttcacctcc ttagttgatt acaaaatatg cgcatcagaa aaacttgttc
    14761 cttttggatc ttttgctaaa tacatttggc gctctagttg ttcttgtaac aactgattat
    14821 catctcccat cgtttgatct tgactatcct cttttactgt aaaatcatag acaatcattt
    14881 cagtaaattt ctcaaattct ttgataaagt gataatcttt gtaaacaaat ggcgcaaaaa
    14941 actctacagg tacatcaaat cctggaaaag catatccacg accaggttga tctttgttgt
    15001 aaaatggctt atttttaaaa tcttctccaa aagtcattaa ataaccaatg ggagacaatc
    15061 gaccaacaga aatacggaat gttaaattat cccgaacacc acttggaaaa tattctgcat
    15121 ctggtcgttg cattgctaaa ataatgtaca tccctgcttg acgacccttt aatactaatg
    15181 gctgaatcaa tttatcgact ctatttctcg cttccatatc caacgaacca taaaaagctg
    15241 cccattcatc aaaaaagata aaatgcggtg gtaacccata ataagcataa tttttccctg
    15301 gttcatattt aggatctttc atggttttca tataggcata ccgttgattc atttctttta
    15361 atgcatttcg taaacaaata atcatttctt catttatttt gtaaaacaca tgtcctttga
    15421 atacatcgac gtcacctaat gctgctaaat ctgccgcttt tccatcgcaa atatctactg
    15481 tcccaacact taaacaagct tgaattaaag aatacaataa ataggttttc ccaccaccga
    15541 ttgcaccagc aatcaacata tttggtgatt gatcatatac ccaatccaaa gttttcatta
    15601 aatgaatacg tccattttct gctatacatt gatcaatagg aatccgacca gaatctgaaa
    15661 ggaatacata attgattcgg gctttttctc gttcttcacg aattaaatca cgcaaaaata
    15721 cttcagataa caaatgaccc atttttaaaa atcgttcgtg aaaacgccca ccatctaaag
    15781 gaaaagaaac cactaattct tgtttctttt gataaaaata aacttttggt aatctcattt
    15841 tttcccttgt ttgtttctca ctaacttgtt tctttgtata aacaaaccga ttgtcaatta
    15901 acattcgaac aagtaactga cgccaataaa cagaggcaaa atatccttct tttagtcgcg
    15961 accaatgcat caaccctaaa attaacccac taaccagcaa caaatacaac acaatactta
    16021 ttccaggaat aagatacatt tgccaattcg aaaacgtact taaacgtgga taaaatactt
    16081 tccaactaaa ccaacctaac agaatcaaaa atggaaacaa aaagaaacca taatagcaaa
    16141 accataaata acgattgaaa tagcgaattc gccgaccacg atacttgaaa aaatgagctt
    16201 tcaattgtca cattcctcct ttctgttttt acttttcctg tgctaaaaac ttttgacaaa
    16261 aagcaactaa tacaggaata tcacatttag taaaaaaaca taatcgaaaa agatttaatc
    16321 gatttttata agggtgtgca cacatattac atattcgtac tatacgaatc caaatcactt
    16381 cctgatgatc ttgtttccac tggtacggta atgaaaaacg aacaagatca cgtaaaaaca
    16441 tacactgccc aataaccgca caaatatatg catttgtttg tacaaaaatc gttggattgt
    16501 ctccaaaata ctcaatcaat tgttggatat tttgataaca agcacaatct ctcaaagtat
    16561 ttgattggtt ttatctgaat tcaaaatctc cccttccttc tatcttagat aaaaagaata
    16621 ggaagactcc ttcctattct ttcaaaaaaa ccttattttt tctctttttc cttagaatca
    16681 acacttggtt gtttttgatc taaacctttt tcttgtgatt gtttcatatt aggattattt
    16741 gttggagctt ctactttctc taatcctttc gcataaatca ctaaatctga atattcacga
    16801 ttgccatctt gtgctggaat atataccaca tgatattctg gatcaattaa tttcacagga
    16861 tcacgtcgtt taaaaccagt caaatctaca tctgctggaa atttaactcg tcgtgcaatc
    16921 tttggttcat ctggatttcc aaaaactaat tgtgctcgct gtgatcgaat ttctcccgta
    16981 taatcgcctg tttcattatc aaatacacga tcagactctt ctaatgcgcc aaagcgtacg
    17041 gctcctaaac aagtttcatc taatgcttct gtaatgtcat aattatttgg aaattttaat
    17101 cccatcttac aaaacctccc caatttttac accagttaaa aagacaccaa catcttgata
    17161 gccagttgtt cgacgctctg tttgtcccat atcatttgtt tcacgactat tcactcgttc
    17221 tttacgaaaa acaaaataag cttcttcaaa tgaaatacgc acctcttttt ctggtaatcg
    17281 taatctttta tcctcttcag ctgtgagata aacaaaaaaa gcaggctgtt cttctcgcga
    17341 acactctgct tgtaattgaa catatcgccc agacacacca ttaatttcct ttggtggatt
    17401 aggttcccgt gaacccaata aaaatacttg acctaaaaac tctttcttca tacgcaatgc
    17461 tttttctgcc ataaagtttt tcctcctctt aaaaaccaaa ataaattcta aatatttcta
    17521 cattacaatc aaacaagcgg ttaaaaatcc taaccgcttg ttcctcatga ctaatctttg
    17581 gctttacgta ctttataaag tgttatacct aaagcaatga ctaatactac taatccaaga
    17641 accataagta tactacttgt ttgttcacct gtttttggca acattggtac aacagtagct
    17701 ggtttaaccc gttgttctgg tttcactggt tgcggtggtt ttggcgcttc cacaatttga
    17761 accgtttgat ctttgtttgt tagatctaca ttgtgtttcg ccacttcttc aaacgtttct
    17821 ttctcatctt ccgttttcgc ttggagccat tccgcaaaac tcaagccttt tcctgcaaga
    17881 ttgcgaccgt ccactaagaa ttcaaataag aattccatca tttctttatc ggccgcaaag
    17941 actttttccg catgagtgcc ttcaaatggt gtgccgtctg gtgttaagta gccttgaaca
    18001 attaagcgat agatttgacc aatcgcaaga ccgtctactt tgacccattc aagcacagtt
    18061 tgatttggca aggccaagat ctcttttccg ccatttttat tcgtcgcttt cgtcgtaatg
    18121 gttggattac tgattttgat ggtttggtca ttgtcggtaa tatctgcgtg agaagccaat
    18181 aatacgcctt ctctaaacag atcttcaaag accacgactt ctttgcctcg caacgcacgt
    18241 gcgtctagaa taaagtctag attgactgta ccatccgttg tttctgccac aaacgttaag
    18301 gtacttgtga cttcttttcc atctactagc actggttttt ccgttacttt gtccattaga
    18361 acaccgttga ctgtgtatgt ttttccaacg accaaatcgt ggtatttcac agcgtctgta
    18421 atggtgactt tcgttaatgg attaacctct ttcaagccag tgggtttatg tgtagccgtt
    18481 gtacgtacac ttgggttagt tactcgtacc gtttgccctt tgtcgttgat gtctttatgg
    18541 tttgctagtt cttcatcatt tctaaaaaga ctttcaaaca cgacgatttc acgaccattt
    18601 atacctgtca aatcaaaggt aaatggtacg atcaccgttt cattttctgc tttggcaata
    18661 aagccaacac tcgtttctac cgtatttcca tgcacaagca agggttcacc ggttgcttta
    18721 tccattaaga tccctttgac caagtatgct tttcctggaa gaatctcggt aatttcaacg
    18781 gtatcgttta aggtaaacaa tcctgttggg tctgcgactg tcaatccgtc ttcaaagaca
    18841 gccgtggtat gaacggttgg attattcact ttcacacttt gggcttcatc gttgatatcc
    18901 gcatgtgctg caacttcaat gccatcacgg aaaagttttt caaaaacaac gatcgtttga
    18961 cctttcagaa cactagcatt aaccacaaat tcaaggtctt ttgtgccatt gactttgtct
    19021 gcaataaagg ccaaggtact tgtgatttct ttgccatctt gtaaaattgg cgcattcgtt
    19081 gccttgttca tcaatactcc tgaaatggtg tattcttttc cgacaaacaa gttggtataa
    19141 gaaacactgt ctttaatgac gatcgtttct aatggatcaa ccactttttc accatcggct
    19201 ttatttgacg cttgtgtttc tagttttggt ggaatcaagt aattacgtac agaaaccact
    19261 aacgtttccc catcatctgg cgcattttgt ttgaatgcaa ccgtgaatgc ttgtttatct
    19321 gtatttggtt ggaaactttc taatggggca ttttccaaga agtaatagtc atcggcaact
    19381 agacctttca ctaaaatttc cccgttttga tcagtggtat attcaccaat tgcttgattt
    19441 ttaccgttat acaaggtaaa gttcacacct gcgagcggcg ttttcttctt gttccattct
    19501 tcatacttga ataattttac acttttccgt gcttgacggt tcgcaaaatg gatcactttg
    19561 atcggttctg tatcattggt aatcgagaac actaatgggt tttgattcaa aacgtaattt
    19621 acgggggctt tgatttcttc caaacgatag cgatcttttc cataagtaag tgtaccgtta
    19681 gttaggaaaa agcctttttc attggtttta aagatatccg ttgtttctgt atcatttgga
    19741 atctccattg tgacataacg attggctaaa gtatcaaaaa tcttgaattc tgtattagca
    19801 agtggaatca cttttcctgt ttcttgatcc actttcacca ctttaagacg ttgttcaatt
    19861 actttatctt ctaaaacata atggaaagtt tgcccatttt cagaaattgt gacttcaagt
    19921 ggttcaaata gtttataccc ttcttttcct ttggtttcag aaagaatgta cgtatcaaag
    19981 acaacggtat ttttaccaaa agtagcataa ccatcattgt ctgtcacaac ggttttcacc
    20041 actttgccag ttgtttttga cgtcaaagtg atttcaatgc cagctaattt tggtttgtta
    20101 tctggattcg ttgaatcgac taaatcttta ttagcaactt tcactaaatc aatggttcct
    20161 ttgatcactt gatcttttgc ggtcgcattg acagtttttc caggttcaac aacaatttca
    20221 attgcttcat cagaaagcac ataacctttt ggcgctttga tttcttttaa aacatatttg
    20281 ccaggatcaa gatctttaat ttctgcctgt actttgccgt taattttttt caaggtaatt
    20341 tctttaatct ttttcccatc tgcttgatac aaaccataga ccgcattttc taaagaagct
    20401 gcaccttgtg ctttcacacc agtttccgca tcttctttaa acacacgaat gtttccttgt
    20461 tttagaattt gtaaattaat aacagacgca ttaggttctg ttggatgtaa cacaccaatt
    20521 gtttgagctc ctggacgacg ataggctaat ggcgcacctt gaactttaat tttattcaat
    20581 tgaatttttg ctttatccgg tgcctctttt gtgactgtaa tccatacttc attgccgtga
    20641 atagatacat tgacaccttt tggtttttga atgtctttca catatttcaa aaaatcattt
    20701 ttattcttta ctttcaacgt ttcgccaatt tttaccgtat gtgtttgatt attaaaagtt
    20761 gctttttcac caaagttatt aatcaaacgt tgaatttctg cttttcgacg atcataatca
    20821 acacgtatat ttttgattgg gatttttgtg cccaaagctt cccaaatcat aaattgtgcc
    20881 gcaatgtatt gttcatcgga tttgtcttta tttgtgacat atcctaaata agagatcaag
    20941 cttgtttttt tccgtacatt tgaatttttc aaatagtttt tcaaagcagt agctgtataa
    21001 acttgacctt ctgcggctga gacaccaggc tcaatacaaa acgccacttg tccattaatc
    21061 tcaaaacgaa ccatataatt taaagtgaag tttagattgt tcggactaga agtaacacca
    21121 atattttcgc cccaatcaat tttccgacca cctaaaacag ctgcattggt tgcacggcga
    21181 acacgaaccg aacgattggt tggtactgtt actaacagtg taggtgtacc tgtttttgga
    21241 atgttctgct cttttttctt ttgattttca atactagccg tagattcttt gtttggtttc
    21301 gttggttgat tttgactagc tgcggttgaa gagattgttt tttcaacgtt ttcctcattt
    21361 tttccttctt tgtcagcagt agccgctggt tgttccactg tttcttctat tcgtttcttt
    21421 tcttccgctt gtttctcaga agactctggc tgcacagaag gcgttgcttc ggcagctttt
    21481 tctccttcta gagtggacgt ttctgaagca ggggttgtaa ttgaggcagt ttcttcaact
    21541 ggttgtgaag taccaggact attccctggg tgttgtgttt ccccaatcgt ctgatcgaga
    21601 gtcgttttgg aattgaccgt tggcgcttct gtcacagggg cctgttcaac agtggcttca
    21661 ccagctggga ccccggcttc tgctggttgg acttctgtca cttcagaagc gacttgggca
    21721 atggcttcca tcgtaggttg tatagccgtc gtttcttctg catgtgcttg agcagttccc
    21781 accaagaaac agaattgaga aaaagccacc aatgcgagtg ctttcaacca attcattttc
    21841 gtttttgcat acacaagaat tcctcctttt cctgagaatt ttatataata aaacaaaacg
    21901 ccgttaccaa aagtgataac gacgtgaact gtctcttcta tttttatctg gcgctcttat
    21961 ctgaacacat ttcatcaatg attttaccct cccactataa ttacagaaca aaaaaagaac
    22021 ctgcttcgtg gaaatcaact gtccatttcc caattggaat accagctttt ttggcaactt
    22081 caccatcacc aaatctcatg ccataccaat tactaccttc atccttaatt tctttccaac
    22141 catatgacat agcttcatct aatgaatcaa aaatcatgac actattgcca attgttacag
    22201 gatctggctt aagatcttct ggaagagaaa tatcaatcat accataatga tccgattcct
    22261 cagtattatt gtcattacta gctactggtt tggttgttct actggcttat tttgatacaa
    22321 caagtactct tattcttctt ttacttctcc gttttcaatt tcccattcaa accactccgc
    22381 aaagtcttgc ttaaaaccat ctactcgata cctattttgt aaataggcat cttctgacgt
    22441 cgtaggatct aaataggtag gtttattctc tggacgagca tagacaccta cccatgtttc
    22501 gtaatctcct tcaaaataac tatctaccaa atagcctttt tttctaagaa tctcccgaac
    22561 ttgattttta attattgata cttgttcaaa accatcactc attctatcac ctcaaataac
    22621 tctagattct cataaatatt cccgacaatt tcaatcctat caatgatctc taacaaatcg
    22681 ctacttatgc ttccccattc gtataagaat ttcccttccg caaaataaat tttccataaa
    22741 cttcttcatg atcgtcccaa ccaatatcac tctcgaaaaa ttccacgttg ttcttatctt
    22801 ttagctcagt aaatgccata agttcaacat gttttccaac ctcgaaacaa tcacaccacc
    22861 catcaaccgt tacacgtgtg ccttcaatac ctaagaaact ttgtcctcgt gtattaaaca
    22921 ctaaaacttt ccgcattttt tgctcattaa ttaatcacgc gcgaaatcta ggaatcacaa
    22981 cttattcgct tcttctttat aaataaaaaa actccaataa tcggaaatta ctatcttttc
    23041 ctcattcctt ttttaaatct tcatacttct tcttatcttt gtcctaacct aataacacaa
    23101 attttgaaga aaaactcagt aaaacctgat tcttttttta tcttcacaac aaccaacttt
    23161 ttcttcagat ccttttacta gaacttgtat acaaaaattg ttgaatgctg atataatttg
    23221 cggctacttg aaaaaacgat agatgtaaaa agataaatag aatcattttt tattctatat
    23281 cagtctatga ttcattgctt atcaatcacc caacccattc gatgccgttt tttttagaga
    23341 aatttaaaaa agaccgtacg aatctcgcat tatccgatta cttttccaga aaagatcccc
    23401 aattctgtat aaataaccct atcttcttgt tggtttcatt ttctcatata agaattcagt
    23461 caatctaccc actatgacca ttactacgtc caacacaact gacatattaa ccacgtaagt
    23521 attccctcac agatctccga atctccatgg tatggattcc catcctgacg ctgcagagga
    23581 caacatattt catgtggctc tgtacccttt tgatttggaa acactacaaa tcaatactca
    23641 tctataactt atcaagcgaa ctttcgctag gcttctagtg attactaaat tgttggataa
    23701 catacatagc ccattttatc tagctgttat tcataacagt tagttcttgt attacttctt
    23761 ccgcttgtgt tgccattgag cttgtcaaaa agttacttag aaaaaagctg ccagtaaagc
    23821 aactttcaac aaaccctttt
//
LOCUS       DAZUMK010000032        23376 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000032 DAZUMK010000000
VERSION     DAZUMK010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.032"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..74
                     /locus_tag="ACG808_001237"
     tRNA            2..74
                     /locus_tag="ACG808_001237"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            78..150
                     /locus_tag="ACG808_001238"
     tRNA            78..150
                     /locus_tag="ACG808_001238"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Phe,seq:gaa)
     gene            155..225
                     /locus_tag="ACG808_001239"
     tRNA            155..225
                     /locus_tag="ACG808_001239"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Gly,seq:tcc)
     gene            238..311
                     /locus_tag="ACG808_001240"
     tRNA            238..311
                     /locus_tag="ACG808_001240"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:gat)
     gene            323..394
                     /locus_tag="ACG808_001241"
     tRNA            323..394
                     /locus_tag="ACG808_001241"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Glu,seq:ttc)
     regulatory      583..662
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            660..1853
                     /gene="metK"
                     /locus_tag="ACG808_001242"
     CDS             660..1853
                     /gene="metK"
                     /locus_tag="ACG808_001242"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFS5046876.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1983..3455
                     /locus_tag="ACG808_001243"
     CDS             1983..3455
                     /locus_tag="ACG808_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFS5046877.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3538..4326
                     /locus_tag="ACG808_001244"
     CDS             3538..4326
                     /locus_tag="ACG808_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5046878.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4393..5061)
                     /locus_tag="ACG808_001245"
     CDS             complement(4393..5061)
                     /locus_tag="ACG808_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFS5046879.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYNLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGIKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5176..6888
                     /locus_tag="ACG808_001246"
     CDS             5176..6888
                     /locus_tag="ACG808_001246"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5046880.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6891..8660
                     /locus_tag="ACG808_001247"
     CDS             6891..8660
                     /locus_tag="ACG808_001247"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5046881.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(8865..9431)
                     /locus_tag="ACG808_001248"
     CDS             complement(8865..9431)
                     /locus_tag="ACG808_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5046882.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(9431..10387)
                     /locus_tag="ACG808_001249"
     CDS             complement(9431..10387)
                     /locus_tag="ACG808_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFS5046883.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(10387..11043)
                     /locus_tag="ACG808_001250"
     CDS             complement(10387..11043)
                     /locus_tag="ACG808_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFS5046884.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            11413..13431
                     /locus_tag="ACG808_001251"
     CDS             11413..13431
                     /locus_tag="ACG808_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS5046885.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFSARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    13501..13707
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13858..16272
                     /gene="leuS"
                     /locus_tag="ACG808_001252"
     CDS             13858..16272
                     /gene="leuS"
                     /locus_tag="ACG808_001252"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFS5046886.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            16620..18173
                     /locus_tag="ACG808_001253"
     CDS             16620..18173
                     /locus_tag="ACG808_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFS5046887.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(18554..20338)
                     /locus_tag="ACG808_001254"
     CDS             complement(18554..20338)
                     /locus_tag="ACG808_001254"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5046888.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNFAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(20353..22074)
                     /locus_tag="ACG808_001255"
     CDS             complement(20353..22074)
                     /locus_tag="ACG808_001255"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5046889.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLQIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            22174..22935
                     /locus_tag="ACG808_001256"
     CDS             22174..22935
                     /locus_tag="ACG808_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS5046890.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         7390 a         4192 c         4858 g         6936 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgtttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
      121 gtgtcggcag ttcgattctg tcccgcgcca ttatgcgggt gtagtttagt ggtaaaacca
      181 cagccttcca agctgttgtc gcgagttcga ttctcgtcac ccgcttttag tttttatggg
      241 cctatagctc agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc
      301 atttaggccc atttatttta ttggcccgtt ggtcaagcgg ttaagacacc gccctttcac
      361 ggcggtaaca cgggttcgaa tcccgtacgg gtcatggaag atactttttg agtatctttt
      421 tctttttatc tgaaagaatg attttaaaaa gcttagcaga gttatcttta tggatgattg
      481 tgctaagctt ttttggacag ttctaatgta aaatattttt atatttttta agcaaaaaaa
      541 tgaaaaaata cgagcagaaa actgttttat ctgctatact tggttcgttg caagttcccg
      601 aaaggatccg caggacggag atgccttgta accgaattta actaggggga atcattataa
      661 tggtagaaag acacttattt acatcagaat ctgtttctga aggacatcca gataaaattg
      721 ctgaccaaat cagtgatgca atcttggatg caattttaaa acaagatcca acagcacgag
      781 tagcgtgtga aacgtctgta acaactggtc ttgttttagt atttggagaa atatctacga
      841 cggcatatgt agatattcaa aaagtcgtac gggaaacaat aaaagaaatt ggttatacac
      901 gtgcaaaatt cggatttgat ggagatacag cagctgtatt ggttgcgatt gatgaacaat
      961 ctcctgatat tgctcaagga gttgatgaag cacttgagat ccgggatgaa gataaaaaag
     1021 acgtattaga tgagattggt gctggtgacc aaggtttaat gtttgggttt gctgtagatg
     1081 aaacaccaga attgatgcct ttaccaatcg ctttgagtca tcgtctggta cgacgcttgg
     1141 cagatttgcg taaatcaaat gaattaacgt atttacgtcc agatgcaaaa tctcaagtaa
     1201 cggttgaata tgatgatcaa ggacaaccgg aacgcgtaga tacaatcgtt atttcaacac
     1261 agcatgatga tgcagtagat aatgaaacaa ttcgtcatga tgtcatcgaa aaagtagtga
     1321 aggaagttat tccagctgaa ttattagatg atcaaacgaa atattatatc aatccgactg
     1381 gccgatttgt cattggtggt cctcaagggg atgccggatt aacaggaaga aaaatcattg
     1441 ttgatacgta tggcggttat gctcgtcatg gtggcggtgc tttttctggt aaagatgcga
     1501 caaaagttga ccgttctgcc agctatgctg cacgttatat tgctaaaaac attgttgcag
     1561 cagggcttgc tcgcaaagca gaagtacagc tagcttatgc gattggtgtt gctcaacctg
     1621 tttcgatctc aatcaatact ttcggtactg gaacggttcc ggaagaagaa ctgattgctg
     1681 cagtgagaga aaacttcgat cttcggccag caggaatcat cgagatgctt gatttgcgtc
     1741 gtccgattta caaacaaaca gctgcttacg gtcattttgg tcgtacagat gtagatttgc
     1801 cttgggaaca tacggacaaa gtagatgcgt tgaaagcaag tttagcaaaa taagttatat
     1861 tcagctggaa cgatcgccaa gtggtggttg ttccagcttt ttttatggca gttaacctct
     1921 tgaaaaagat agtaattatt tatctatttt tagatcctgt cataatgaaa gggaggaata
     1981 agatgaaaaa agaaacaaat gttactttgg tcacagtgag tattttcatt gcaacgttca
     2041 tgacggctat tgaaggtaca atcgtaacaa ccgctatgcc aacgattgtc ggttcattac
     2101 atggaatgga aatcatgaac tgggtatttt cgatttattt gctgacaaac gctatgctta
     2161 ctccgattta cggaaaactt gcggataaag tgggacggaa accaatcttt atttttggga
     2221 cattattgtt tattatcggt tcgttatttt gcggtttatc tcaaagcatg attgggttga
     2281 ttgctgctcg tgcgattcaa gggatgggtg ctggggcaat gatgccagta gctttgacga
     2341 tcatcggcga tctttattct attgaaaagc gagcaaaagt tttaggtttg aatagttcag
     2401 cctgggggat tgccagtgtt tttggaccgc ttgcgggagg attcatcgtt gaaacgatca
     2461 gctggcactg gatctttttt atcaatgtgc cgatcggaat tctattaatt attttgattt
     2521 ggctatattt aaatgaacca aaaatcatac atgagtctaa accaatggat atttttggta
     2581 gttttagttt gatggccatg cttgtctcat tgctgttagg gttccaatta ttaggagacg
     2641 aaggaatgag tccgcttgtt tctgctttgc tgctaggtag tatcgtaatg ttaggtattt
     2701 ttgtttttgc cgaaaaaaga gctaaagatc ctgtcatcat gatgcattta ttcaaaaatc
     2761 cactatttgt tgtggtcaac ttggctgcag cattggctag tggttttttg atgggaatcg
     2821 atgtttatat ccctatgtgg atgcaaggtg tattagggct gaatgcagca attggtgggc
     2881 tggtattagc accattatca ctagtctgga tggtcggttc tttccttgca agcaagtgga
     2941 tgactcaaat gaaaactcat tgggtcttga gaattggact actaatcgca gctattgggg
     3001 gcgcttggtt gtatcttatg cctttcagaa gtaactattt atggtttttt gctatatcaa
     3061 cggtcatcgg tataggctta ggtgtgacca tgactacctc gactgtcagt gcacaaaata
     3121 gtgtatctcg tgaccagctt ggtgtagcaa cttcttttaa tacgttagtt cgaacaattg
     3181 gtcaaacagt catggtttct atttttggtt tattgataaa tggggtaact aaacgagaac
     3241 ttgcagcagc aaatttaaca aatgatgcag atatcatgaa taaactggtg aatccacaaa
     3301 cggctaaata tattgatgcg gatattttgt taccattaag aaaaattctt tatgatgggt
     3361 tacattccgt ttatctagtt ggcttgttac tcgtgatcat tgctttggtc ttaacgcttt
     3421 tcgttcatga aaaaagtggt aaaataaaac agtagtttcg gttaagatat gtacgtagcc
     3481 gaatcattca aatttaaagt tattcaaaaa taacttctta ctaaggaagg cgaaaatatg
     3541 gcatttattc aagcaaatat ttattcgaat gtgttagaaa tggaagtcaa tctaaatgtg
     3601 atactaccgc aaacaacaaa aaaggtgatt ggcaccagtt cagagcaaga gacaacggat
     3661 atacctgttt tatatttact tcatggaatg ggtggcaatc atagtgtatg ggaaagacgt
     3721 acgtcgattg aacgttatgt agcaaactac ggattagctg tcatcatgcc atctactgat
     3781 cttggctggt acacagatac agtttatgac atgaagtatt ggacattcat tgcagaagag
     3841 ctacctgaga tttgtcatga attattcccg caattaacaa gaaaaagaga aaaaacatat
     3901 gccgctggat tatcgatggg cggatatggc gcgttaaaac taggccttgc aaaatcagaa
     3961 aatttcgcag cagtaatctc tctttcaggc gctgtttctg taggaaatcg aatggatgat
     4021 cttttgatgg ttcgtaaagc atctttttgg gaagggatct ttggaccatt ggatcaaatc
     4081 gaaggttcaa aaaatgatcc gcaatactta ttgaaagaac tagtaaaaag cggaaaacca
     4141 gttccaagat tttatatggc ttgtggggaa tctgattttc tttatcaggc aaatcaggaa
     4201 atggtatctg ctatgcagga acaaggcttg gcggtcacct ttgaacaagg acctggcgat
     4261 cataactgga tcttctggga ccaatggatt caacgtgcat tggcgtggct aatggaagaa
     4321 aaataaaaaa agagtcttaa aaagatagct cctatagaga tcacaaacat agtgatctct
     4381 ataggagctt ttttactgct cgttttctct tcttttattt tgttggcata agccttcaat
     4441 ataggtttgt gcactcgtta gaaagagtgc tttttcattc aattctgcat gggactgcat
     4501 actttcttta attccctcta attgattttc aactacataa ttacgcataa atagaactgt
     4561 ttctttcaaa aattcatttc cgtccataat tttttgtcga agctgctgtt catgctgctg
     4621 atccattaac tgtccaatca ataaattgtg gagagaggca atggcctgat gactttgggt
     4681 gactgaaaat ccttcacgaa caagtaattg gatcatttgc tccaaatgtt ctaatcgact
     4741 cggaaaagcg ggcacagtat tgatttctaa ttctgcccca caagggaaac gtgtataaag
     4801 gttatagtaa ttttccatat actgcagcaa ctgttcattc caaggcaaag ttggatctgg
     4861 aagcgctaat tcttcttcca ttgtttcagc cattttttgc aagagctgtt gtttgttttt
     4921 gaaataccaa tataaagcag gtgcttgaac atgaattctg tttgcgatgt ttcgcatgga
     4981 aagctgcgaa agagcttgtg tttcagccag gatatcaaag gcggcctgaa tgatgattgc
     5041 ttgagaaagc tttatttcca ttttatcaac ctcgtgtatt gcattatctt tttaaacatg
     5101 gtaaattgtt agtcggtcta attttacagt gttaaattag tcctgtcaat ttaaacaaag
     5161 gggtgtaatc aacgaatgaa attaatctta cgacatgcga aaaaatataa agtagctgtt
     5221 tttatttcct tgctgtccgt agcagtcatg gttgcagcag cattatggca acctaagtta
     5281 ctacagcaag tattggaagc tattattaca gaagataatg atgaaatgaa aacaattggt
     5341 atttacctga tcagtctggc tttgctagga ttagccgcag gtgtaacgaa cacgattttc
     5401 tctgcaaaag tagctcaagg cgtaagtgca gatatccgag aagaagcatt tcgaaagatc
     5461 cagacatttt catttggaaa tatcgaacag ttctctgctg gaaatcttgt tgttcgtcta
     5521 acaaatgata tcacgcaaat ccaaaattta gtgatgattt ctttgcaatc actttttcga
     5581 attccattct tatttattgg tgccttcatt ttagctatga tcacgatgcc gcaattatgg
     5641 tggatcatcg tagcgcttat cgttacggta tttctgatta cggcactttc atttacaagg
     5701 atgggcaagc acttcatgat cattcaaaaa ctgattgatc gtgtgaatag cattgcaaaa
     5761 gaaaacttac tagggattcg tgtagtgaaa tcttttgtac aagaagaaaa ccaattgaag
     5821 tcattcagca aagtaagtga ggacttaaca aaacacaaca tcatagtcgg tacattattt
     5881 tctgtgatga tcccgtcctt catgctggct gccaatttgg ctgtggttgg ggcgatcttt
     5941 tttgtgagcg atcttgttaa agatgatcct acgttaattg gtggaattgc ttcattcatg
     6001 aactacttga tgcagattat gatggcaatc attattggcg gaatgatgat gatgatgact
     6061 tcaagagcag ccgtatcgct gaagcggatt gcggaaattt tagatgctga accagatctt
     6121 acttatttag acgttccaga acaagagttg acgggttctg tcacttttga ccatgtttct
     6181 tttcgttatc ctggtgagga tacagatacg cttaaagata tcagcttttc tatcaaacct
     6241 ggagaaatgg ttggtatcgt cggggcgact ggtgccggga agtcaacact agcacaattg
     6301 atccctcgtc tatttgatcc gacagaaggc aaagttgaag taggtggtgt agatcttcgt
     6361 caagtaaatg aaaagagttt aagacaaacg gtttcctttg ttttgcaaaa agcaatttta
     6421 ttctcaggaa cgattgctca aaacttgcgc caagggaaaa agaatgcaac agaaaaagag
     6481 atggaacatg cttcttctat tgctcaagca aaagaattca tcgaaaagtt atctgatcgt
     6541 tacgaagcac caatagaaga acgtagcagt aacttttctg gtggtcaaaa gcaacgttta
     6601 tccattactc gtggcgttat cggtaatcca aagatcttga tcctagatga cagtacgagt
     6661 gcactggatg ctcgctctga aaagctggtc cgagaagcac tcgatcgaga tttgaaggga
     6721 acaacgacga ttgtcatcgc acaaaaaatt gcttctgttg tcaaagcaga ccgcatctta
     6781 gtattggatg aaggcagatt agtcggcgaa ggaacccatg aggaattggt agctggaaac
     6841 cgtgtctatc aggaaatctt tgaaactcaa aaaggaacgg aggagtaagt atgactgatc
     6901 taataaaagc aagtaaattt ttctatcact atttgaagcg gtacaaactc tcctttttct
     6961 ttatctttat cacagtgatc attgctacct atctacaagt aaaggcacct caatacatcg
     7021 gggaagcttt tcaagaatta gctaattacg taggaggctt gatgcaaggc gtagacgata
     7081 aaagcaaatt tgtttctgta atatggaaac tcttgctgtt ttatgtacta gctagtgcag
     7141 ccaactttat ctatagtatt ttgttcacac aagtagtagg gaagtcaacg aaccgaatgc
     7201 gtatcggtct attcaacaaa ttggaaaaat tgacgatccg tttctttgat tctcatcaag
     7261 acggtgaaat tttaagtcgt tttaccagtg acttagataa tattcaaaat agtttgaacc
     7321 aagctttgct tcaagtcatc acgaatgctg ttttacttgt cgggatcttg atcatgatgt
     7381 tccgccaaaa tgtagagtta gcttgggcta caattgcttc cactccagta gccgttttga
     7441 tagctgtctt gattatccgc aaagcacgga aatatgtgga tcttcagcaa gatgaagtgg
     7501 ggaaactaaa tggttatatg gatgaaaaaa tcagcggaca acgtgtcatc atcacaaatg
     7561 gcttacaaga agaaacgatt gaaggattct tagagcataa tcaaagtgtt cgcgaagcaa
     7621 ccttcaaagg acaagcttat tctggtctac tatttccgat gatgcaagga atgtcactgg
     7681 tcaatacagc gatagttatc tttttcggcg gctggttagc attaaatggt gatttggaac
     7741 gttcggtggc attaggctta gttgtgacat tcgtccagta ttcacaacaa tattaccaac
     7801 cactgatgca gatttcatct ggatacagca tgatccaatt agcgattaca ggggcaagac
     7861 gattgaatga aatatttgat gaaaaagatg aagtcaaacc agaaaacggt aaagcatttg
     7921 atggtgttga aaaaggtctt gcgttgcagc acgtagattt tggttattct ccagaaaaac
     7981 tagtcttgaa agatgtctcg atcaatgtgg acaaaggaga aatggttgct cttgttggtc
     8041 caacaggatc ggggaaaacg acgatcatga atctgctaaa ccgtttttac gatgtcaatg
     8101 atggttcagt agtcatcgat ggaatagata tccgagatta tgatcttgat gcattacgta
     8161 gacatgtagg aatcgttttg caagaatctg tattgttctc cggaacgatc aaagacaata
     8221 tcacattcgg caaaccggac gcttcagatg aagaagtcat caatgcagca aaacaagcga
     8281 atattcatga cttcatcatg acgttagaaa aaggttatga tacagagata tccgaagaaa
     8341 ataacttatt cagtacaggt caaaagcagt tgatcagtat tgctcgaacg atcatcacaa
     8401 atccatcttt attgattttg gatgaagcaa caagtaatgt agacacggta acagaagcaa
     8461 ggatccaaaa agcaatggat gaagcaatca aaggaagaac cagctttgtg attgctcacc
     8521 ggctgaaaac aatcttgaat gcagatagaa tcgttgtctt gcgtgatggg gaagtcattg
     8581 aagaaggcag tcatcacgag ttattagcag aaaatggatt ttatgcggaa ctttatcata
     8641 atcaatttgt atttgaataa agaggttgtg aaaaagctgt cctgcatcaa gtaataagaa
     8701 cagccaatca aaaacagctt ctcatgtttt tgatttggtt ggacttatta ccgcagatat
     8761 aagcttttga gcaccgtgaa taaagaggtt atgaaatcaa cgctctaacc tgagtattag
     8821 agaaaaaaac gcaaaacata tcatttatgt tttgcgtttt ttatttatct ttttttctca
     8881 atacaatata aaataggcgg ctggttcttt tggttgataa accgataatt cagcacatta
     8941 tatgtttcct gtggcagttt ggaacaaaaa gaatcaacca tatccagttc tttttctcca
     9001 ccttcatgac catagtagac gacaaggatc aatcgtccgc gaggcacaag tcgtttcaat
     9061 atttcttcca tagctgtgcg tgtagtctct ggcatagtga tgattgcttt atcacttttt
     9121 gggagatacc ctagattgaa aatccctgct ttgagatttt gttgttcagc tatcacttct
     9181 cctaacgttt cgtggccttg tagaaacaat gagacacgtt cttctaactg acgctgttct
     9241 agcctttcac gagtagcatc gattgcttgt tgctggatat caaaactata gacatgacca
     9301 gtttttccga tgtgttctgc tagaaaaacc gtatcgtggc cattgcccat cgttgcatct
     9361 acgacgtgat cccctggttg caagatttct tgcagtaatg tatggctgaa atgcaaagca
     9421 gtttgcaaca tcagacgctc acctttttcc gtacatcata ttttccttgg aagctatctc
     9481 tgcgaagaag ttcttcatca attgcattca agacttccca ttttttcaag ctccacattg
     9541 gtccaatcaa ggaatcccac ggcgcatccc ctgtcaaacg atggatgatg atctcttttg
     9601 gtatcatttc cagctggtcg caaatgacat tgacatagtc aggacggctc attaattgaa
     9661 ggcgaccttc atgatagtcc cgcaacattc tcgttttgcg catcagatgc atcaaatgga
     9721 gcttgattcc ttggatatct gaatctaaaa tggtgcgtct gacattttct ttcatcattt
     9781 ctaaactttc gcctggtaat ccgttgatca aatgcgtaca aacacggata ttatgtttgc
     9841 gtaatttagc cacgccatct aaataagttt gataatcatg tgctcggttg atcagttttg
     9901 aagtttcttc aaaagttgtt tgcaatccaa gttctaccca taaatacatc cgctcgttca
     9961 gttcagcaag gtaatcaact acttcatctg gcagacaatc cggtcgtgta ccgactgata
    10021 atcctacgac acctggtaaa ttgactactt gctcaaatcg ttctttgatt atttcaactg
    10081 gcgcatgggt gtttgtaaaa ttttggaaat aaacgatgta ttgttggaca tgaggccatt
    10141 tttgatgcat catgtcgatt tctttctgga attggattgg aagagggtct tcaggtgcaa
    10201 cgatcatgtc tcccgaacca gatacactgc aaaaagtaca accgccatgg gcaacagtcc
    10261 catctcgatt cggacagtca aatcctccat cgatcggcac cttgaaaatt ttcccgccaa
    10321 actcatttcg taaagcgtaa ttccaagaat gatatctttt atttccttct gaataaggaa
    10381 aagtcattaa aagtccttct ttctgtaaga gttatctatt tgttttcttt tttctctata
    10441 cactggataa gtcaggcaca gccaagaaag actgagtaag tagccgccga tgatatcagt
    10501 aggaaaatga acgcccagat aaatgcggct tatcccgaca cttactataa taaacatcat
    10561 cagcaattga agcaatgtgc gtaaggttct tttttgaaca aaagctggaa gcagaaatag
    10621 taaagaaccg taaagaatca tactgcctgc tgcatgtcca ctagggaaac tgtaacttgt
    10681 ttcaactact aagtgcgcca atgaaggacg ctctcgcatg aaaaatagtt tgatcaccgg
    10741 gttgatgatt ccagcaatta caataaatcc agaaacgaac caaaatgttt ctgccctttt
    10801 tttcttatac cacatccata aagccgcaca agcaaataaa atgatgacgg taagaggatt
    10861 accgaattta gtcataaaga gttggtagct gttcagatca ggataagaag ctctgacaat
    10921 gcctgtaaac cactgatcga aaccagaaag ccacgacaga tggaacttta cgatccagcc
    10981 caaaatgaaa aacaggacaa gaaagaagct accagccacc tgaaatgatg ttttatattt
    11041 catacaattc ctcctttgaa gcaatgactt attatagcat ttttggtcca gcgaaaaaaa
    11101 cggtaaagag ataaaaccaa tgtatgattc ctctttgaat aaccaatatt cttataaaaa
    11161 aaataactgt aatcggaaag acattttttg gtaatgcaaa tgaaatgtta gtgaaataaa
    11221 atcagtgttt ttcgaaatcc ttttaaacaa gaacgtagac gtaggaagaa cttgaaaatg
    11281 aacagaagtt gttcattacg gtaaattaca atatgattac aaaaaacaac aaagctctta
    11341 aataactttt aataaatatt aagagaagtt ataattatta ataatagata aattgaagtt
    11401 ggaggataga agatggaaaa cattgcaaga aaagaacgga gacgtttaga acagaccaaa
    11461 cgttttcgaa aagcaaaacg aggggcagca atcgtgggaa ctgcgatggt aggctgttct
    11521 gtcgctgccc cattagtaca gccggttcct gttcaagcgg atgaaacacc tgttcagttc
    11581 agcgcaagaa tcaatacagc ggcatttatc acagaaattg ctacttatgc tcaaccaatc
    11641 gctcaagcca acgatctata tgcatcagtg atgatcgcac aagcagttgt agaaagcggt
    11701 tggggcagta gtgcgttatc tcaagcacct tattataatc tttttgggat aaaaggcagc
    11761 tatcaaggac aaaccgtcta catggatacg cttgaatttt taaataatca atgggtaaat
    11821 aaaaaggagc ctttccgaca atatccttct tttgctgaat ctttcaatga caatgcctat
    11881 gtacttagaa atacttcatt tggcaacggt tattactatg ccggtacttg gaagagcaac
    11941 acgaaatcct atacagatgc gacagcttgt ttgacagggc gttatgcgac tgatcctggt
    12001 tacgcaggca aattaaataa tatcatcgca acttacggat tgacccaata tgatacacca
    12061 gctagcggaa acgcaggcgg tggagcgact acaggtggca ataatactgg aagtgcaggt
    12121 agtactggcg gttcaagcaa ttctggtaat acaaactcag gttcagcaac ttcgaccact
    12181 tatactgtcc aatctggtga ttctgtttgg ggaatctcaa ataaattcgg tatcacaatg
    12241 gatcaattga tccaatggaa caacatccaa aacaatttca tttatccagg acaaaaactg
    12301 acaatcaaag gtagccagga aaatggctct tcgacgaata attccggtaa caataccaat
    12361 tcttcaggaa atgctggtac atcaaacgga ggacaaacaa ctggtgcgaa atacactgtt
    12421 caatctggtg attctgtttg gaagatctca aatgatcacg gaattacgat ggctcaattg
    12481 attgaatgga acaatatcaa gaacaatttc gtttatccag gacaacagct aatcgtatca
    12541 aatggcggtt cttctaatgc tggagcaaat actggctcaa atactgggaa tacaagtggc
    12601 tctagttcgt ccagctcaag ttcttctaat acagcttcag gaacgaaata tacagttaaa
    12661 gccggagact ctgtttggag cgtctcaaat aaatacggaa ttacgatggc tcagctaatc
    12721 caatggaaca atatccaaaa taactttatc tacccaggac aacaactgac tgtttcaaac
    12781 agtggatcaa caaacagcac gtcaggtaat acaggttcgt caaccaatac aaataacagc
    12841 agttcagtta atactggcag cagttcgtct caaggcactt ataccgtgaa agcaggtgaa
    12901 tctgtttgga gcgtggcaaa taaaaacggc atcacgatgg atcagttaat cgaatggaat
    12961 aatatcaaaa acaacttcat ttatccagga cagcaattga tcgttaaagg tggaacttcg
    13021 gcaaatacaa atacaggttc aactacttct gcagcgaaac caaatacacc gaatacgtct
    13081 gctacgacaa gtacatcatc cactggtaat acgatgtata ccgtgaaagc gggcgagtca
    13141 gtttggagcg tagcgaacaa acatcatatc acgatggatc agttaatcga atggaataat
    13201 atcaaaaata atttcatcta tccaggacag caagtgattg tgaagaaagg ttcatctcaa
    13261 tcaacaaacc aacaatcagt tactggttcg aaaacctata ctgtgaaatc tggagaatct
    13321 gtttggagtg ttgctgacag tcatggaatt acaatggctc aactaatcga atggaacaat
    13381 attaagaaca actttattta tccaggtcaa acgttgattg tagctaaata aaatagctgt
    13441 tcgaagaaat aacgattgaa ttccacaaaa atatttgtta ctataaggct acttaaatat
    13501 gacaacactt gaaaaggaag tagtaaaaag actgaagttt cagagagcgc atggctggtg
    13561 agaatgtgca cggatgcttt tgaactcgcc tttgagttgc ccttgctgaa tacccgagta
    13621 agcaagcggc ggtgacggcc gttacaacgt tcgaggcctt ttcactaagg taaaattagg
    13681 tggtaccgcg tagattacgt cctataaaaa gagcaatctt tttataggat ttttttattt
    13741 cttttgtgaa gtttgagaaa acaaagcaaa aggaaataaa aataaacaga ttctgccggt
    13801 tttttcgtta tatttataat agaaacaagt gttttcaata gaacaggagg acagcaaatg
    13861 agttacaatc ataaagaaat tgagaaaaaa tggcaaaaat actgggcaaa aacgaatact
    13921 ttcaacaccc atgatgatcc tgaaaagcca aaattttacg cactggatat gtttccctat
    13981 ccatccggtc aagggctaca tgtagggcat cctgaaggtt atacggcaac ggatatcctt
    14041 tcacgtgtga aaagaagtca aggatacaac gtgctgcatc caatggggtg ggatgcattc
    14101 ggtctgcctg ctgaacaata tgcgctagat acaggaaacg atccagctga gtttacccaa
    14161 aaaaatatcg aaacattccg acgccaaatc aattctttag gatttagtta tgactggaat
    14221 cgcgaagtga atacaactga tccggagtat tataaatgga cacaatggat ctttacgaag
    14281 ctttatgaaa aaggtctagc ttatgaagca gaagtagcgg taaactgggt acctgaatta
    14341 ggaacagtta tttcaaatga agaagtgatc gatgggaaaa gtgaacgtgg tggctatgat
    14401 gtagtcagaa aaccaatgcg ccaatggatg ctgaaaatca ctgcttatgc tgatcgctta
    14461 ttggatgatc tagaattagt tgattggcca gaaagcatta aagaaatgca aagaaattgg
    14521 atcggtcgtt cagtaggagc aaatattgaa tttaaagtgg ctggtacgga taaatcctat
    14581 accgtattta cgacacgacc agatactcta ttcggtgcaa cttactcggt tcttgcacct
    14641 gaactagatc ttgttagaga aattacgacc cctgaacaaa aagctgctgt agaagcttat
    14701 atcgaagaga cagctaaaaa atcagatcta aagagaacgg atcttgcaaa agaaaaaaca
    14761 ggagtattta ctggtgctta tgcaatcaat ccagttaatg gcaaggaaat cccaatctgg
    14821 atcgccgatt atgtattatc ttcatacggc acaggagcca tcatggctgt tccagcacat
    14881 gatgaacgtg actttgaatt cgctcaaact ttcggactag aaattcttcc agttatcgaa
    14941 ggtggagatg ttgaaaaagc tgcttacaca gaagatggta cgcacatcaa ctctgaattt
    15001 ctagacggca tgaacaaaca agaagcaatc gataaaatga atgaatggct agaagaaaat
    15061 ggcgtaggga aaaaagaagt cagctatcgc ttacgtgact ggctattctc ccgtcaacgt
    15121 tactggggtg aaccaatccc aatcatccat tgggaggacg gaacagtcac agctgtccca
    15181 gaagaagagc taccattacg gttgcctaag acaaaaaaca tcaagccaag cggtactgga
    15241 gaatctccat tagcaaatat cgaggagtgg gtcaatgtcg ttgatcctgt aacaggtaaa
    15301 aaaggccgtc gcgaaacgaa tacaatgcca caatgggcag gaagctcatg gtattacctg
    15361 cgttatatcg accctcataa caaaaaagaa ctagcagatt atgaaaagtt gaagcgttgg
    15421 ctaccagttg acatctacat tggtggagca gaacatgcgg tacttcattt actatatgca
    15481 cgattctggc ataaattctt atacgatatc ggtgttgttc ctacaaaaga accattccaa
    15541 aaactataca atcaaggaat gatcttaggc gaaaacaacg aaaaaatgtc aaaatctcgt
    15601 ggtaatgtag tcaatcctga tgatgtggtc gaaacttacg gtgcagatac actgcgcatg
    15661 tacgaaatgt tcatggggcc attagatgct tcgattgctt ggagcgaaaa tggcttagaa
    15721 ggaagccgta aattcttgga tcgtgtatgg cgtttgatcg ttgatgagaa caataaaatg
    15781 cgtgaccgta tcactacatt gaatgatggc aagcttgata aagtgtatca tcaaacagtg
    15841 aaaaaagtga cagaagacta tgaaaatctt catttcaata cagcaatctc ccaattgatg
    15901 gtctttatca atgaagccta taaagtggac gctctgcctt atgaatatat cgaaggattt
    15961 gttcaactac tcgcaccgat tgctcctcac atgggtgaag aactatgggc aatccttgga
    16021 aatgatggtg gtatttctta cgcaccatgg ccaacatacg atgaagcagc acttgttgaa
    16081 gacgaagtag aagttgtttt ccaagtaaac ggtaaagttc gtgcgaaaag caatgttccc
    16141 cgagacttag gaaaagatga gctagaaaaa gcagcattag ccaatgaaat agtgcaagaa
    16201 tatattgaag gcaaaacagt cagaaaagtt attgcggtac caaacaagtt agtcaatatc
    16261 gttgctaatt aattggctct ttgtcaataa ggactgatga gttgtgcaaa atcaaatctg
    16321 ggccagaatg aacttcattc tgtctcagat tttttgttat ttcaaattga ttaattgatt
    16381 gatcaaaaag gttttgattt tcatgttctc aaacacgttc aatgtctttt tcttctgaat
    16441 ggtcttgtaa gagatctctg taatcatcaa atttttgtct attaatctta gtattttttg
    16501 atgtataaac tacctgaaat tgagagtaca taccaaattc ataaattttt tattagtggg
    16561 actagtgatt gtatggattt tgacgcataa tgaatgaagt atgagaaaag gagagacaaa
    16621 tggatcaaga actatttatt atcgaagtat tatcagctaa acaaaaaatt atcgagaaag
    16681 cgaaaaaaag agcgaaaaga ataatgttca agcttttaag cttagtattt ttctctggtt
    16741 tgcttttttt cggagctgta tcgttcctat atcgccatat gagtgcagta gtccgttcag
    16801 ctgaagggat gcaaaaatta aataatttgt atcacttttc gtggggagtt ggcggaataa
    16861 caattgttgt gcttattgtt tttcttctac ttatttggct ccgttaccat caattgtcgc
    16921 gtcatttcga tcaatggctt agtcaaagag cacaacagtt tttacataat aaaccaactt
    16981 ctcaagatga tgactatctt tattttgaga agacttcgac gagtcaagct tttcggatac
    17041 aaaaaagtga ttgccagtta ttgctgagat cacttgatga tttacctatc tacttaggcg
    17101 aaagcaaatc aaaattcgga atgaacacat tccatatttt tttactagaa aacgaaccca
    17161 gatgggaaaa aagccagtct tcgcctggtg gatacctgac aactaagatg agtacattag
    17221 tcactggttt tttcctcatt cttttagctg gaacaatcta ttatgggaga tacactcagc
    17281 caaaaatgtc ttctcatata aatgctgctt ctcacatgac aatgaactca actaacgata
    17341 cgcatataca aaagacaaat gaagggttgt tagctgatcc taagcaaaag gtaaatttga
    17401 caacacaaaa gataaatgat ctcaaacttg atacagagac gcactctctc tatatgacaa
    17461 cagatagtgg tgctaattgg gtatttgtgc cgattgattc gcaatggttg cgttttggcg
    17521 aatacaccct gacaacgggg ctgattcctg aaggttattg gatggacaac acatatgata
    17581 tttccgccga tttctcatgg tttatctatt cttctgggga tgatgtacgt ctgctgactt
    17641 caagagataa tggtaaaaca tggcaaacgt ctattatcac ccaatcagtt cagccgatac
    17701 gttatcgaaa agcggtattt tatggcaacg atggtctatt tatctgtaca tcagccagtg
    17761 ggatgtctgc agaaactttg tacatttaca ctactatgga tggcggaatc acttggataa
    17821 aaagtggaag tacaatgatc aatcagccag tacaaaatgt cagcttcgtt acaacttctt
    17881 taggttttgt ttcgacacgt gaaagtttgt tttatacaaa caacggagga cgctcattca
    17941 aagaatcagt tctgtcgatt ccggaaaact atcagacagg ggggatagat ctttttcaat
    18001 caccaaatga agttactcaa ataagcgcga atacgttaga gacaaaattt tatttgttga
    18061 aaaaaggacc aatcgatgca gggaaaatgt ttgcctgcct cttccgttca actgataacg
    18121 gggaaacatg gcaattcgtt cagcaactat cgcaagtaga acaaggagat taaagaagag
    18181 agattgtgag cctgacgttc agcaccgagt cacaagaaac aaattcaaaa aacagcttct
    18241 catatttgtt gaatagtgta actcgtgatt cggagttgac aagagcaaca agaagcgatt
    18301 ttgaagtata gcttctcgta ttttacaaga tcgtgtctta ttgccgatag gttgtctctt
    18361 gaacaccgtt tattcgtaaa aagggattgt gacaagtctt ttgtcactac ttaccgaacg
    18421 aaactgaagg agctatccaa aaagtatcat tagtattatt ctttgtgttt ctagtggcac
    18481 cactagtaca tttaaaaaag ctatcgatca tggactaatc atacatgatc gatagctttt
    18541 tagataatgt ttgttacgct atctgaaatt gactattata aagactggca taaagcgttg
    18601 gtgattggag tagttcagaa tgtgttcctt tttcgacgat atcgccattc ttcattacaa
    18661 ggatcatgtc tgcattttca atcgttgaca aacggtgtgc aatcacaaaa cttgtcctat
    18721 cttttgtcac agcttccatt gcttgctgaa tcaatgcttc tgttcgtgta tctacgctag
    18781 aagttgcttc atctaaaata acgacaggag gattcgctag aatgactcga gcaattgtca
    18841 ataattgttg ctgtccttga gataacgtgc cgttttcgct agaaatgatc gtatcatatc
    18901 cgtctggcag tgttcgaata aagtgatcac attgtgcgat ttcagccgct tcgataattt
    18961 gttctcttgt ggcatccatt ttcccgtagg cgatattatc agctaccgtt ccttcaaaca
    19021 gccaggtatt ttgtaaaacc atcccaaaag aacgacgtag ttctccacgt gaaagcttgg
    19081 taatatcaag accgtcgaat tcgatctttc ctttattcag ttcataaaaa cgcatcagca
    19141 gattgactaa agtcgttttt cctgcaccag ttggaccaac gatcgctacc gtttgttttg
    19201 gctttactga aaaatcgact tgattcatca atattttatc tgatgtgtaa ccaaactgta
    19261 catttttgaa agctattttt cctttcggtt gttctaaaag tcttgcatct ggaaggtctg
    19321 gaatttcatc tgtttcatct aatatctcaa atatcctatc gattgctgcc attgcggatt
    19381 ggatcgagtt gatcacataa gaagcggtag aaattggttc agaaatctga ttgatgtact
    19441 gaagataagc ttgtaagaag ccgatcgtca caccacctga tagaacaagc atagcaccga
    19501 ataccgcact tatgatgaag gcgagttgat taatgaaacg aatggccgga tagatagcaa
    19561 agttcataaa ttgagcacgt ttaaaagcgt tgtattgctt tttatttact tcgtgtatag
    19621 cagcttctgc atgagcttgc tgattgaatg ttttgaccac tagattacca gctaagattt
    19681 cttccattcg attatttaaa acacccagtt catactgatt ctgttcagaa atctgtttgt
    19741 ttttgttcgc aatcttagtt gtaacaattg aactgccaac gatcagcaat aagacgagca
    19801 aagtaagctt gctttcgata taaaacagca tcagaccagc agcagctatt gttacgacag
    19861 aagtaaaaaa ttgattgata cctgtcaata acacttgaga aagttgattg agaccggtag
    19921 ttgtccgact aatgatgtca cctacttgat gattgtcaaa aaacgacatc ggtaatgttt
    19981 tgaatttttt tgtgatttct tttcgcaccc tcaatgtgat cttttcgctt aacgctgcca
    20041 ttgtccgttc ctgaataaat gaagtgatgc tgcttaagac tgaacagccg atcagcaata
    20101 agacagggaa caacaatgct tgtttgatat cactgaaaga aagctgtcca atacctgttt
    20161 ttcttatttc tgataagaga tcatcgatcc cgattcccat gatcaagggc atggctacga
    20221 ctaataggtt cccgaataaa ctgcatagaa acaatccgta aaaatattta tgttcaggtt
    20281 tgatcatttt ccaaaaacgt atcaaagcac gcgtgttcaa cgtttttttc ttttccatcg
    20341 tatcccttct ttctatattc cttgtgattt tgcaaactcc tgataagttt ggcaattaga
    20401 cagtagttct tcatgagtac cttgtccaac gatttttcct tcatctaaaa caatgatgtt
    20461 atctgcttgt tggattgtac tgaggcgttg agcaacaatc acgagagttt tctccgacat
    20521 gtgtttatga agggctgctc ttaatgctgc atctgtttta tagtctaaag cggagaaact
    20581 atcatcgaat acatagatct ctgctggttt gatcaacgct cgagcaatac atatccgctg
    20641 tttctggccc cctgaataat tagaaccgcc ttgagcaaca aagctgtcca atccgtcagg
    20701 aagggtgtcc actagttctt ctaattgagc gatcttgatc gctttagaca actcttctgt
    20761 tgaggcatat tcatttccca tctgcaagtt gcttaaaatt gtcccgctga ataagaaagc
    20821 cttctgaggg acataactga tgtgggagcg gatctgttct tgagtcattt gagtaagagg
    20881 aataccacca agggttactt ttcctatcgt agggtcacta agtctcaaga gaagtttcgc
    20941 caaactactt tttccagatc cggtcccgcc aacgatcgct gttgtttttc ctttaggtat
    21001 tgtgaaatca atatcttcta gaactggatc agagatttca gtatatttaa atgtgacatg
    21061 ttcaaaagca gctgcatttg tctgatcaaa ggttggttca gctactgttt tgatcgtctc
    21121 ttcctcaatt tcgatctctg cttgcagtat ttcttctatc cgtcgtaatg aggcaaacga
    21181 tttagggata atgaccaata ccatagctgc gatcatcaag taagcaagtg tcagcataga
    21241 gtattcgact acagcagtca cgacaccgat ttgtaatgct ccttctgcta caagatttcc
    21301 gccaaaccac aaaatagaag cataaacgat tcccatgagc aaaaaagcaa ttggagtgac
    21361 ccaagcaaat attcggttca ctttgatcat ttgattagca tagtcagcaa agctcttatt
    21421 cgttcgcttt tcttcatatg attgattatc aaaggcacgg atcatattaa tcccagtaaa
    21481 gaattctctc aatgtgatgg tgatccgatc catttttggc tggatactca aagataaagg
    21541 tgcccctcgt ttcatcagtc gataaacaat ccatacataa aaaacaacag agatcaatgt
    21601 aacccatgtt agtaatagag aataacgaaa catcatgaga atggtgaaaa cgcaaataac
    21661 cggtgctggt aagatcattt ggcaaaataa aacaatcatt tgctggacat tatcgacatc
    21721 attcgtcatt cttgtcaaaa gagatccaga actaaaacga ctgatatcct tcattgacag
    21781 ctgttggaat ttacggaaat atttctcccg cgtttggaat ccaaacctag cggaagccaa
    21841 tgctgaaaag taactagagg caactgcagc gattgttcct agaaacgcta caagcagcat
    21901 ttcaactcca atttttttta taactgaggc ttcgccactt gctatacctg tgtcgatcag
    21961 ctttgctact aattttggta cacctaatgt accggcgatt tgtaaacaga tcatcacaaa
    22021 ggtaaggaga gcgagtcctt tgtttttctg taagaatgtt tttaagatag acatattatt
    22081 cctccttttt tcctgatact tttcgattct ataccttcaa gtaacttgaa ggtcaataga
    22141 aaaaagaata aactgaaaaa gaggtgagaa aaaatgaaaa aaatgtatac gattggccaa
    22201 gtatcggaac tatttgattt gccaaaatca acgatccgct attgggatga acaaggatta
    22261 atccgttcaa gtagacaaga agagaatgat tatcgattgt ttgatatcga tgatattttt
    22321 atgctttatg atatcgtttt ttatagaaaa ttagatatac cgatcaaaca gatgaagaat
    22381 ttatatggaa aaacattgac ggagttatat gagacacttg atgaaacaga aagaagaatc
    22441 cataaagaac tggtggtgat gaaacaaaaa caaaaagaga taagggagca aaaaaaacag
    22501 ttgaaattga tgatcgatac aaacgaggaa gaatttcctg tagaagagat cccttttgac
    22561 tgtatgatct caacggagtt tgaagatatt gtggaaatca aaaaatttct acctaactat
    22621 tcaagctttg ggatgatgag tatgccagct tccagttcac agacgatgta tggatttttt
    22681 atcgacccta gtgaagtaca tttatttact caagacgtga tttgggagaa aaaagatacg
    22741 gctgtttatc gtcgattctt attgaaatcc gagatgaacc atgcggaacg taataatatt
    22801 ttagaaatca gagaaagaat gtatgaaaaa ggatggaaaa caggagaagt gatcggccag
    22861 tatctattga cgaatacgga tgaaaacaat atcagaactg aatattatca tgcatggata
    22921 gaaatgaaaa aataggaaat atgtgtttaa gataactcga aaatgatggt tgttttagtt
    22981 ataaagaaaa attttctggt tcgattacgg gtttaatgtg ggggattatt agtcgtggat
    23041 agatagaatt ttagtttttt agaggaaata tgtctttaaa aatggaatgg ttgcaattct
    23101 tagcgatata tacgatgtaa ttgtcctctt aaaaaagtat ggaaaaataa ttgtattggg
    23161 tgttatgtat aattcatggt gaactgagac taatgagaag aaaaattcat attgttatga
    23221 gtgagattgt atcagatgag aatgataaat taaataggtg cataaaagca ttacacttaa
    23281 aatacattgt tgcttacatt attacaatat gttaataagg caaaaataaa tagatggatt
    23341 gaataaacat attttttaat caaatagtcc ctactg
//
LOCUS       DAZUMK010000033        23207 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000033 DAZUMK010000000
VERSION     DAZUMK010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.033"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(185..310)
                     /locus_tag="ACG808_001257"
     CDS             complement(185..310)
                     /locus_tag="ACG808_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFS5046891.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(444..1337)
                     /locus_tag="ACG808_001258"
     CDS             complement(444..1337)
                     /locus_tag="ACG808_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFS5046892.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(1519..2445)
                     /locus_tag="ACG808_001259"
     CDS             complement(1519..2445)
                     /locus_tag="ACG808_001259"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFS5046893.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(2530..3291)
                     /gene="pflA"
                     /locus_tag="ACG808_001260"
     CDS             complement(2530..3291)
                     /gene="pflA"
                     /locus_tag="ACG808_001260"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFS5046894.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(3535..5766)
                     /gene="pflB"
                     /locus_tag="ACG808_001261"
     CDS             complement(3535..5766)
                     /gene="pflB"
                     /locus_tag="ACG808_001261"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFS5046895.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(6043..8493)
                     /gene="parC"
                     /locus_tag="ACG808_001262"
     CDS             complement(6043..8493)
                     /gene="parC"
                     /locus_tag="ACG808_001262"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFS5046896.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(8505..10550)
                     /gene="parE"
                     /locus_tag="ACG808_001263"
     CDS             complement(8505..10550)
                     /gene="parE"
                     /locus_tag="ACG808_001263"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFS5046897.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(10716..11150)
                     /locus_tag="ACG808_001264"
     CDS             complement(10716..11150)
                     /locus_tag="ACG808_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFS5046898.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            11277..11915
                     /gene="plsY"
                     /locus_tag="ACG808_001265"
     CDS             11277..11915
                     /gene="plsY"
                     /locus_tag="ACG808_001265"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFS5046899.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(12047..12922)
                     /locus_tag="ACG808_001266"
     CDS             complement(12047..12922)
                     /locus_tag="ACG808_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFS5046900.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(13004..13795)
                     /gene="codY"
                     /locus_tag="ACG808_001267"
     CDS             complement(13004..13795)
                     /gene="codY"
                     /locus_tag="ACG808_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFS5046901.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(13813..15213)
                     /gene="hslU"
                     /locus_tag="ACG808_001268"
     CDS             complement(13813..15213)
                     /gene="hslU"
                     /locus_tag="ACG808_001268"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFS5046902.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(15227..15775)
                     /gene="hslV"
                     /locus_tag="ACG808_001269"
     CDS             complement(15227..15775)
                     /gene="hslV"
                     /locus_tag="ACG808_001269"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFS5046903.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(15812..16717)
                     /gene="xerC"
                     /locus_tag="ACG808_001270"
     CDS             complement(15812..16717)
                     /gene="xerC"
                     /locus_tag="ACG808_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFS5046904.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(16915..18291)
                     /gene="trmFO"
                     /locus_tag="ACG808_001271"
     CDS             complement(16915..18291)
                     /gene="trmFO"
                     /locus_tag="ACG808_001271"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFS5046905.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(18260..20338)
                     /gene="topA"
                     /locus_tag="ACG808_001272"
     CDS             complement(18260..20338)
                     /gene="topA"
                     /locus_tag="ACG808_001272"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFS5046906.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHGHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(20460..21317)
                     /gene="dprA"
                     /locus_tag="ACG808_001273"
     CDS             complement(20460..21317)
                     /gene="dprA"
                     /locus_tag="ACG808_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFS5046907.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(21373..22140)
                     /locus_tag="ACG808_001274"
     CDS             complement(21373..22140)
                     /locus_tag="ACG808_001274"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFS5046908.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(22133..22993)
                     /gene="ylqF"
                     /locus_tag="ACG808_001275"
     CDS             complement(22133..22993)
                     /gene="ylqF"
                     /locus_tag="ACG808_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFS5046909.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            complement(23087..>23207)
                     /locus_tag="ACG808_001276"
     CDS             complement(23087..>23207)
                     /locus_tag="ACG808_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS5046910.1"
                     /translation="FIQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT         6076 a         5015 c         4000 g         8116 t
ORIGIN      
        1 tttatgtggc aaagaaaaaa caaacaaaat aaaaagcatt ttgaagcctg actattacga
       61 cttcataaat gcttttttag ttttaaacac ttcaatctaa aagaataacc caagattgga
      121 taaattatta atgactcgcc attcgtttct tagtgattct ggattatcaa atggacttcc
      181 taatctaatc agctagttct tcccataaaa tgccatactt gactgaatca taatattgat
      241 tatttaaaaa tctaactttt cttatcacta cttcctttgt cattccacat ttctcaccat
      301 ttttttgcat tcctttgttt ccagaccatg tagtgaatct aataggtggt agataggcaa
      361 aatactcaaa taattctttt aaccataaag gaagagaaag gctcctcctg caagagtcag
      421 tataattttt gcttactgct ctcttaggtt ttactcacta aaactgttcc atattctttg
      481 atataagaaa atgcatgcgg tataaatttc ttgctcagct cagaaaagat ttgtcgttta
      541 agaaaggcca tttcctccat tgtcaatgaa ggatccaatg ttggtaattc aatctcaaaa
      601 ttgttttttt cgacaatagg taattctaat gtgaatgatt cttcttcctt tatagtattc
      661 gtcacaagta gagactgatg gaaaatacta ggccggcgat ttttatattt attcttacgt
      721 atcctgtcaa cgataacttc tatttgatca aaaaagtacg attcatttac gtctccaata
      781 agttcggcta attctttgaa tgccttcgtt acaggaagct gattcttctg tacaaaaaag
      841 taattagcca aagtaagcgg gaagtgttct atagaggaaa gtgtgataaa acggtacttc
      901 tcatttcctg cagttgtcct cagcaattct gtctctcggc tgtttcttac tccataaaaa
      961 tacgcttctt tccgttcatc ttcgaaacaa tacgcccaaa taacttctcc catcgctaat
     1021 ttttgttcat caacagagag tctcttcaat tgttcagcta tggtttcagc agccgaagtg
     1081 gcttgttgca agtaatcttt aggatcaaat attggaaagt tcaaatgata tcttcgttct
     1141 tccctcttga ttaatcctgc atcgatcaat aaatcaagaa aatgggaaaa tttattttgc
     1201 gaaaattctt ttttcaattc tcttagaatc gtttcttgtt ctcgatgttc atatagataa
     1261 tcggtcaatg aagaaaaaag cggatcatcg gtcagttttt gataaaagtt ttccttattt
     1321 tttgaataat agttcatata tgttctcttt tctatccgtt ttattttact taaaaggctt
     1381 ttacgtaaaa aggaggaaga acaagagtca taattgactg ttattctcct ccatttcttc
     1441 ggcctttagt atgttctatg tttcttttta tatcaatcgc ttatccattc attttgaatg
     1501 cttcatacta ccatttgatt agctaaatac agccgtcaat tgaggtacga cttgtttttt
     1561 acgtgaaacg acacctttaa gcaaagcacg gttgttggtc aaagttgttt ggaaagcttc
     1621 ttctactttt tcttttcctt taccagcaac aagtaattct gaatcactgt ctaagatgtt
     1681 tgtgacaact aatacgaaca gatcgtagcc atttgctttc atttcatctt ccattgctgc
     1741 ttctagctct gcttgtcgat ccatcacttc attaaggtcc actgtattga cttgagcgat
     1801 acgaacgttg gaactgccca ttgggaaact ttttgcatcc aaatcaagaa gaaccgcagc
     1861 tgatttatca cttagatttg ttcctgcttt caacatttca agtccataag tgtttagatc
     1921 gacttcagcg attgctgcaa gttcttttgc agctgcttca tcttcaggcg tacatgttgg
     1981 tgatttgaac aacaatgtat ctgaaatgat agctgagagc atgattcctg cgatttcttt
     2041 aggaattgtt acttggtttt ctttatatag ttttaaaata atcgtacttg tacatccaac
     2101 tggttctgca cgatagtaca atggattagc tgtttcaaag tttgcaatac gatggtggtc
     2161 tactacagct aaaatattta cttcgctgat atctgaaata ctttgttgga attcattatg
     2221 atccacaagc atgacatctg cattttctgc tttagctgac tctactactc gtggtgcaga
     2281 tagctgaaag tggtctaatg cgtattgtgt ttcttcatta ggtgtaccta gagcaactgc
     2341 ttctgtttct ttacctaatt cattttgcaa gtgtgaaaac gcaatggctg ccccgattgc
     2401 atctgtatct ggattttggt gaccaaaaac taaaattttt gacatgtcat tcgctccttt
     2461 gtgatttctc ctatttaata atagcaaaaa aacggaaaga agcaatactt atcttctttc
     2521 cgatgcgacc tatcgcgtta aatattttgt atactcttct gtatgaagca atcgtttagc
     2581 attttcgaca cgttcttttg taggtggttc gatcccttct aatggatatt tcaaaccaag
     2641 tgtttcccac ttgtattttc ccatcgtatg ataaggaaga atctctactc tgtctacatt
     2701 tgacaaggtt tgaataaagt tgtctagtcg gatcaaatat tcatcataat cacttcgctc
     2761 aggaactagt acatggcgaa tccaaaccgg tttgttgatt tttgacaaat attgagccat
     2821 ttctaaaata ttttcattgt cccaatgcgt tagtttttta tgctcttcat tatcgatgtg
     2881 tttgatgtca aataacagta aatctgtata cttcatcaat tcttgaaaac gtgaaaagaa
     2941 aggttcttca tacgtaaaag gttttccgca tgtatccaat gtagtatgca tcttccgctc
     3001 ttttgctttt ttaaatagtt cgatcaaaaa atcgatctgc aatagaggtt ctccaccact
     3061 aacagtaata ccacctttat ctccccagta aggtctgaag cgttctgctt tatctaaaag
     3121 ttcatctgct gtgtattctt tccctccgcc aatattccaa gtatctggat tgtgacaaaa
     3181 ttggcaacgc atgcggcatc cttgcataaa cacgacaaag cgcagtccag gtccgtctac
     3241 tgaaccgaaa gtttcgatag aatgaacata gccgattgtt ttttcttcca tttaagttca
     3301 catcttttct ttgatttcat tttaagtaca agttagctgt cgaagcgaag ctttctgaac
     3361 aaaatttttc gcaattgccg cttcgttatg tgtaaaattt acagggacgg aacatacaag
     3421 tcctgcccct gtaaatcgtt tctttcaaat aagcatactg attattcgat tttgatgcga
     3481 tggctattca acgtcctttc gacgctaagt cgaaaaactt ttttcacgaa ctttttacat
     3541 tctgtcgtgt gaagttctca tcaagacatc taattgttgt tctcttgtca agtcacggaa
     3601 tttaactgca taaccagata cccggatcgt caagttcgga tatttcattg gattttccat
     3661 tgcatcacgc aataaatcat tactgaatac attgacattc aaatgataag ctccttttga
     3721 gaagtatcca tctaagacat tacgcaagtt ttcgattctt gtatcttgtt cttttccaag
     3781 acctgaagga ttgattgttt gggtatttga aattccatcc aatgcacagc cgtaattcat
     3841 tttagctgtt gagttcaggc tagctaataa cccactttgt tccgcattgt aacttggatt
     3901 tgctcctggc gcaagtggtt ttccagcttc acggccatca ggagtatttc ctgtattttt
     3961 accgtaaaca acatttgaag taatcgtcaa taatgaagtt gttggttttg agttgcggta
     4021 agttttatga cgtttgattt gtcccatgaa gtattccaag atccatgaaa ctaattcatc
     4081 tgcacggtca tcatcatttc cgtatttagg gaagtcgcca tccacttcaa aatcaactgc
     4141 tattcctttt tcattacgga ttgcttttac ttttgcatat ttgatcgctg acaagctgtc
     4201 tgccgcatga gagatcccag caatacctgt agcaaatgtg cgttgcaagt cagaatccat
     4261 caaagccagc tgagctgctt cataagaata tttatcatgc atataatgaa tgatgttcaa
     4321 cgtattgaca tatagttctg ctaaccaatc catcatattc atgaagttat gtttcacttc
     4381 ttcaaagtct agtatttctg atgtgattgg acgatattct ggtccaactt gggcgccagt
     4441 cacttcatcg actccgccat tgatggcata taatagtgtt ttagccaagt tagcacgagc
     4501 gccaaagaat tgcatgtctt ttccgacaac tgttcctgat acacagcaag caatcgcaca
     4561 atcatcagaa ccccattgtt cacgaagctg attatcattt tcgaattgaa tcgaagaact
     4621 ttcgatagcg attctgctag catatgttct gaatccttct ggaaggtgtt cagaatacaa
     4681 aacagtcaag tttggttctg gtgaaggtcc catatttgtt aatgtatgaa gcagacggaa
     4741 atcattcttt gtaactaatg aacgaccatc taatcccata cctgcaatgg ataaagtagc
     4801 ccagataggg aatcctgaga ataattggtt atattcaggt gtacgcgcga atttgaccat
     4861 acgtaatttc attactaagt ggtcgatcaa ttcttgtgct tctttttctg tgattactcc
     4921 attatccaaa tcacgttgga tatagatatc aaggaatgct gagatacgtc cgattgacat
     4981 cgcagcacca ttttgtgatt tgattgctgc taaatatccg aagtatagcc attggacagc
     5041 ttcttttgca tttgcagctg gtttagaaat atcatagcca tatttcgcag ccatttcttt
     5101 catttgcgct aatgcacgat attgttcagt gatttcttca cgtaaacgaa tcacttcttc
     5161 actcattgta tgataaccag tcatatcatg atcttttttc ttttgttcca tcaaatagtc
     5221 gattccatac aatgccacac gacgatagtc accgatgata cgtccgcgac cgtaagcgtc
     5281 tggaagccct gtaatgattt tgtttttacg agcagcacgc atttcagcag tataagcgtc
     5341 aaatacacct tgattatgtg ttttgcgcca gtcagtgaaa atatgtgtca attcatcgtt
     5401 tggtgtatag ccattgcttt ttagcgcatt gttagccata ttgattccgc cgaacggcat
     5461 gaatgcttgt ttcaaaggtt tgtctgtctg tagaccgacg attttttcca aatctttatc
     5521 caaatagcct gcatcgtgtg aagtaatagt tgatacgatt tctgtgtcca tatcatagac
     5581 accgttacgc tcgaattgga tatcatttaa cttttgcaat tcatcccata atttttctgt
     5641 tgcttctgtt gggccttcca aaaagctgtc atcaccgcga tattcagtgt agttattttg
     5701 aataaagtct ctagtgtcta cgccttcttt ccatttggta cctttaaatc cattccattg
     5761 ctccattgta tggacctcct aaatttctgt tttatgtaac agtttttacg tgattagtat
     5821 aacacagcac cctatctttg caagggtttt cttgtttttt atttaacaat ttttatgttt
     5881 tatttacata tatttaaatc aacgttgaca taccaacatt taagcattgt gaacactttc
     5941 atcttttttt gcaaacgtat aaggatttat taaaaatgaa tgaaaaagag acgccacctg
     6001 tattaataca gatggcgtct cttcattaaa atactacaac agttacgact cgatgaaaat
     6061 ctcaggagca tcgataactt cgatgacttc tccacctttt ttctcatcca tcgcaaatga
     6121 tccattcgat gttcgatcac ctatagggaa atcagttggg tcaatcgtta actgctgccc
     6181 tttttgattc atcaaggtta tttcttttga tcttccttcc gacatataga taatacggtg
     6241 cggatttttt ttcagctcac gcatgaccgt cactccccgc ttcgcacgtg aagtttggct
     6301 gatttcttga gcaagcatgc gtttaacagc tcctcgctga gtaacgatta cgatcggcgt
     6361 atcaccttct gctaacacaa gcagcccgtt gataatccta tctccatctt tcaaattcat
     6421 agacttcact ccagctgcct ttgctccaac aacgggaaca tctgttaaag gatagcgcaa
     6481 acccattcct tgatgcgtta ctaagaaaac atctaagcct ggctgatctt ctttcagata
     6541 cacagcaatc agttcatctt cttccgcctt tagcttcata cagctgagcg gacggctctt
     6601 ataggtgcgc catggttcga actctgtcat tcgtgtttgc ttgatcaatc cattcttgct
     6661 gatgaataca aatgttttct ctgcttgaat tttttgatat gggaaaacag ctaaaatcga
     6721 ttcgtctaca gacagattgg agattgtttg agaaatgtgc tcaccagcat ctttccattt
     6781 caaatcgggt agttcatgga cagggcggta gatcacattt gctttatttg tgatcagaag
     6841 aacatggtcc aatgtactca attcacctga gtatagcaag tagtcgcctt ctcgcatccc
     6901 gatttcttct ggtttggaag cactataaga acgaaggctt gttcgcttca catagccttc
     6961 tcgcgtcaca ctgacaatta cgtcttcttg tgccactaag acttgcgtat caattttgat
     7021 ttcttcgatt tcgtcttcga ttgtcgttag acgagcggaa gaataatttt ttttcacttc
     7081 tcgcaattct tttttcataa cagagaaaag ctctttgtca tttgaaagga ttttattcaa
     7141 ctctgtgatt tgcgcaatca agctttcgga ttccttttga agctctgtga tatccgtgtt
     7201 cgtcaaacgg tataattgca atgtgacaat tgcttctgct tgttcttctg taaattgaaa
     7261 gacgtcgact aaattctttt tggcgtcttt tttgtcttta ctttcacgaa tcgttgcgat
     7321 tacttcatcc agaatcgata aagctttcat caatccttct acgatatgct gtcttttttg
     7381 agctttggct aaatcgaatt gactacgttt tgttatgact tgctttcgat gctcgatata
     7441 acttcttaaa atgtctttca gtccaacttg atgcggagtc atatggtcga tagcgaccat
     7501 attgaaattg taattgatct gtagctcagt gtttttgaac aaataattca aaattccttg
     7561 agcattggcg tcttttttca attcgacgac aatttgcaga cctgttctat cactttcatc
     7621 tcgtacttca gctattccat cgattttctt attcaaacgg atctcatcca tttttttcac
     7681 caaggtcgct ttatttactt catatgggat ttctgtgatc acgatttgct gtttattacc
     7741 cttcattgcc tctatctttg ttttcgaacg aagaataact tttcctcgtc ctgtttcata
     7801 agcttttttg atttcatctt ttccttgcaa gatccctcct gttggaaagt caggtccagg
     7861 aatatattcc atcaattttt caaggctcgc ttgtggatga tcgatcatat agattgtgcc
     7921 atcgatcact tcagctaagt tatgagtcgg gatttctgtt gcatatccag cagatatacc
     7981 tgtcgatcca ttgacgagaa ggtttgggta tcgagctgga aggactgttg gttctttttc
     8041 tgtatcatca aaattccaca cgagatcgac tgtttctttc tcgatatctg ctagcatctc
     8101 tccacttaac ttggataatc tggcttcagt ataacgcatc gcagcaggtg gatcaccgtc
     8161 catactcccg ttattcccgt gcatttcgat cagtacttcc cgcaacttcc agtcttggct
     8221 catgcggacc atcgcttcat aaatactgat gtcaccatga ggatgataat tccccatgat
     8281 atttccgaca gattttgctg atttacggaa tcctttgtca tacgtattgc catctttgtt
     8341 catggcaaat aatatccggc gttgcactgg cttcaatcca tcacggatat caggcagtgc
     8401 acgttcttgg ataatatatt tggaataacg tccgaagcgg tcacccatca cttcttctaa
     8461 agttaattct tgtacttctt gtcgattttc catcaggtga cctcctattc tacttcaaat
     8521 aactgattgt tttcattttt atctgctcgt tcttcatcta ataaatcatt agaaactgaa
     8581 ggagagattt cagtatcctc ttttttatct aagatactgc catcttcttc gagcgtaaat
     8641 tgcacgtgat tttcgatcca ttttcttcga ggttctactt tgtcacccat cagtgtggtt
     8701 actcgacgtt cagcttgcgc agcatcatcg atacggacgc ggatcaacgt acgggaagtc
     8761 ggatccatcg ttgtttccca caattgttcc gcattcatct ctccaagtcc tttatatcgc
     8821 tgcagcatat atcctttgcc gactttttta atcattgctg ccaattcgtc atctgtccac
     8881 gcatattcaa tgacttgctt ctttccttgt cctttggata ctttatataa aggcggtaac
     8941 gcgatatata ctttcccagc ttcaatcagc ggtttcatat aacgataaaa gaaagtaagc
     9001 agtaatactt gaatatgggc gccgtctgta tccgcatcgg tcatgatgat tactttgtcg
     9061 taattacagt cttcgataga aaactcagga ccgactcctg caccaatcgt atagatcatc
     9121 gtattgattt cttcattttt caagatatct tgcattttag ctttttcggt attgatgact
     9181 tttcctcgta aaggtagaat tgcttggaac tttctgtctc ttccttgttt agcagatccg
     9241 ccagcagaat ccccttcgac taaatacaat tcatttttct tagggtttcg ggattgtgcg
     9301 ggtgtcaatt ttcctgaaag aagagattcg ccttttttac gcttcttccc atttcggctt
     9361 tcttctctgg ctttacgtgc tgcttctctc gcttctctgg ctttgattgc tttacggacc
     9421 aacatctggc tcatttcgct attttcctgc aaataaaagc ccatttgttc actgatgacg
     9481 ttatctacta cggtacgagc aactggcgta ccaagttttt cttttgtttg cccttcaaat
     9541 tgcagaagat tttcaggtac acggatagaa agtacagcag ctaacccttc tctgaaatca
     9601 ctaccttcca gattcttgtc ccgttctttc agcaaaccaa cttttctcgc atattcatta
     9661 taagatttcg tcatcgccgc tttcatacca gcttcatggg ttcccccatc ttttgtccga
     9721 acgttattga caaaagataa aacattttct gaataaccgt cattgtattg ataagcgacc
     9781 tctacttcga taccttcttt ttctcctgaa aaataaacaa ctggtgtcaa tgtatctttt
     9841 tcttcattca gataatcaac aaattctttg attccttctt cgtaatgaaa aatttccttg
     9901 actggttctt ctccacgaag atcagaaaga gagattttaa cgccttttag aagaaaggca
     9961 gattctctta accgttcggc taaaatttca tatgaaaatt tcgttgtact gaatattgta
    10021 tcatctggta aaaaatggac agtcgtcccg tttgcttttt ttgtttttcc tatctttttc
    10081 aaagtaccgt caggtttacc gccgttttta aatttctgct gatactctac gccatctcta
    10141 acgatcgtta caattagcca tttcgataag gcatttacca cactggctcc tacaccgtgc
    10201 agtccaccag aagttttgta ccctccttga ccaaactttc cgccagcatg cagcacagta
    10261 aaaatcactt caactgttgg tattccggaa gcatgcattc ctacaggcat cccacgtccg
    10321 ctatctgcaa cagttatact attgtcttca tggatggtca catcaatctc actgccataa
    10381 cctgataatg cttcgtcaac tgcattatcg acgatttcat agactaaatg atgcaagcct
    10441 cggctgtcag tggatccaat gtacatccct ggtctttttc taactgcctc taatccttcc
    10501 aatacttgga tggacgagtc attgtattcg ttgtttactt tttttgccaa ggcaaacgct
    10561 ccttcaataa tactttgcgg caaaaccgca ccacgattat catactagaa aaggtcgtga
    10621 aaaaaaagag ggtagacgag aaatcaattt taattatctc ctaaccgctt tttcgacatt
    10681 cccctctatc gatcatctct tctttgtggt catttttatt tttggttttc tttcaactta
    10741 ccaagttcaa tcttaataca acgatccatt acgatatctg tacgtcctgc ttttcttagc
    10801 atctcagctg cttctttgtt ttcaatccct aattgtgccc agaacacttt agcatcagtt
    10861 ttcaaaaaat cacctgctat ttcaggtaaa aattcactcc gtctaaatac atctacgata
    10921 tcgattggat catcgatttc tgtcaaattc gcatagactt tctcgcctag aatttcttca
    10981 ccagccaata atggatttac tggtatgatt ttatatccat tgtcttgcaa tacctgtgca
    11041 accatgtaac ttgttcgttc tggtttattg ctcaagccta cgacggcaat attttttgct
    11101 tcttccaaat agttgaaaat cgtttcttgt gttggatttt ttacagacat ttctgccacc
    11161 ccttgctttt ctttttattc catctattat acttgtatct ttcatttttt caattgttcc
    11221 tgttacctat tcatgctaaa ataaaggata gttttcaact tgaaagaggg taaaaaatga
    11281 aattgatctt attacttatt ttagcatatc ttttaggttc tattccttcc ggtgtctgga
    11341 ttggcaaatt gtttttcaaa aaagatatcc gacaacacgg gagcggtaat actggaacga
    11401 ccaatacatt tcgtgtatta ggcaaaacag ccgggattgt tgtcctgctt atggatattt
    11461 taaaaggtac attggctact tgtttgccga tgatcttcca cctttctggg attaaccctc
    11521 tttggtttgg ggtatgcgcc attcttggcc atacatttcc gatttttgct gggttcaaag
    11581 gaggaaaagc tgttgcgaca agtgcaggaa tgcttttagg atttaatcct gtattttttg
    11641 tttattcttg tatcatcttt atcgttagtt tattttgtac aagtatggtg agtttgagca
    11701 gtatgatcag tgcagtattg atcactttat ctacgatcgt ccttccttat gtagctcctg
    11761 tgattttggc taagcccaat tggttactga cgatcattgc ctttttagtt tcttccttta
    11821 tcttttatcg gcataaagac aatatcaagc gtatcagaaa ccatactgaa agtcgaatcc
    11881 cgtttggttt gaatagcagc aagaaaaaat cctgatactt tttcattcca aagagataaa
    11941 acgggagtgt gacaaatcga tgtcacactc ccttattact tacaggatag cttttttaca
    12001 caccttggta aacggtgttc cctgctaact tctcggtcac aacctcttac ttcactgtga
    12061 tggaatattt tgtcttgaag atttcatggg cacctagttg attgatccct actttttcca
    12121 tcaagtttcc actactgttc accgtgtcag cgattccgca ccacggttcg atacaaacga
    12181 aaggcgcttc ttttggataa ggagaccaga tccctacata aggcatatct ttatagctta
    12241 aagttacgct atgggaagat ttttcacttc gaatggcgta cgcatttaga ccccttgttt
    12301 caaaaaccaa ggcatcttgc tcaaaaagtt cacgattcaa agccagattc gtatttgtct
    12361 gacctaacgt cttttgatcc atatcgatga atgggccttt caatggtagg cgtaatcttg
    12421 atttcaatgg agaaaatgcc agatagaaat cttcaaatga caatccttct tcaagaggta
    12481 cattgaaagc aggatgtcca ccgatagaaa agaacatttc tttttcaccg gtatttgcca
    12541 cttgataatg aaccgtgatg ccgtctcctc ctaattcata gctgatcact aattcaaaat
    12601 caaatggata cttttctaat gtctccggcg tactccttaa gatgaaacta gccatttctt
    12661 gtccatgttc aatgacttca aaatccatat cccgagcaaa tccgtgttgc cccatttgat
    12721 atgttttccc ttgataaaca tactgatcat cttttaaacg tccaacaaaa gggaataaaa
    12781 ccggtgcatg tctgccccaa tacgctggat caccttgcca gatatactct aaaccatgtt
    12841 cttttgattt cagactgatc aattccgcac cgtgttcagc gatttcggcg atcagctggt
    12901 cattttgaat aataacagtc attgccattc ctccaacttc gacttacttt ttaaaatggg
    12961 aaggctggaa caaacatttc tatctgcccc agtcccctta cttctaataa ctgtgttttg
    13021 ctaactcgtc tttaaaacgg gaattcaata cttttaaata agttcccttc atgcctaacg
    13081 aacgagattc aatgattcca gctgattcca attttcgtaa agcattgacg atcacagaac
    13141 gagtgatgcc gatactatcg gcaatattag aagcagtcag acgaccttca tctccatcca
    13201 atgcttcaaa gatagcttgt acagccttca attcgctata agataatgtg ttgattgcca
    13261 tttggacagc cgttgcactc cgtacatctg cttcgatgct tcgagacttc tgataaagga
    13321 tctgcatacc gacaaccgta gctccatatt ctgctaagac tagatcgtca tctgcaaacg
    13381 attgttccat acgagataag ataatcgtac ccaaacgttc gccagctcca aagatcggaa
    13441 cgacagtcgt cagaccaaat ggatatttct ctcttgactc taccggaaat gcagtaatat
    13501 cactagtaat tgggatatta gcttcagttt tcatcaactg tgccactgta tcagtataag
    13561 attcaggaaa ttgtttctca acaaacattg ttttgattcg ttcattgttc acatcgtgac
    13621 gttcattata acctagtaat actccgtcac ttcctatgat ataggtgttg ctgtcaagaa
    13681 taccacctag tgtgacagcc attttatcgt aaggcaaatc tgcttgcatg tcaaaagtat
    13741 tcttttgctg caaaagtgtg tttatcttgc gggttttcgt tagcaagtct gtcattgtcg
    13801 tcctccgatt ttttataaaa tatagcgact taaatcttca tttgccgcaa ttgagccaag
    13861 tttgtcatcc acatatgctt ctgtgatcgt tatttctccc atttgcatat ctggtgcttc
    13921 aaataataga tcttctagta gtttttccaa aatggtatgc agtctgcgtg cgccgatatt
    13981 atctgtgtca cggttgacat cgaaagcgat ttgagcgatc cgttcaattg cttcttttgt
    14041 aaacgtaact gacacatttt ctgtaccgat caacgcgacg tattgtttta ctaaagcatt
    14101 atttggttcg gtcagaatct tcacaaaatc ttcagctgtc aaatcatcta attctacccg
    14161 tatcgggaag cgtccttgaa gttctggaat tagatcactt ggtttagaca aatggaaagc
    14221 tcctgaagca atgaatagga cgtgatctgt ttggatgatc ccgtattttg tgtttacttg
    14281 tgaaccttcg acaattggca atatgtctct ttggacgcct tctctggata cttctccaga
    14341 ttgctgtgat ttagaagtga tcttatcgat ttcatcgata aaaataattc cactttcttc
    14401 cgctaatttg attgcctcac tatggatatc agcatccttg actaattttg ctgattcttc
    14461 cttgatcaat aactctaaag cttctttgac tgttaccgtc cgttccactt ttttcttcgg
    14521 tgtcaaagca cctagagttt catttaggtc gatccccatt tgctccaatc cattgttcat
    14581 cattggtgca ggtttctttt ggtcttcaac ttcaatggtt acttcacgac tatccagtaa
    14641 acccttttgt agttgttcca agactgtacg gcgattgatt cgaatatctt ctgtcacttc
    14701 ttcttgcggt tcagcggtat tttgctgact agcattaaaa agattcatca tttgttcaaa
    14761 ttgattagtg gattgtttct gttctttttt gataccaggt accaagattt ttaccaaacg
    14821 tttttctgca ttttttttag cacttgtgta tacacggctg tactgctctt tttctacgat
    14881 ttggatcgca ttttctacta aatcccgaac cattgattct acatctcttc cgacataacc
    14941 gacttcagta aattttgttg cttcgacttt gacaaatggt gcattgacga tttttgccaa
    15001 acgtcgtgcg atttctgttt taccgacccc tgtaggtccg atcatcagca tattttttgg
    15061 tgtaatatct tgttgcattt tttcatctaa ctgcaaacgg cgatagcggt tacgcaaagc
    15121 aacggcaaca gatttttttg ctgcgtgctg tcctacgata tattcatcta attcacttac
    15181 gatttgtctt ggtgtttgtt ttaattcata catatctact cacctcttac agttcttcta
    15241 cgataatatt atgattagta aagatacaga tatctgcggc aatatttaac gcattttctg
    15301 caatttcttt cgcactcatg tcgttttgag catagttttt cattgctcgg gctgcagcca
    15361 acgcaaaatt ccctcctgat ccgatagcta agataccatc atctggtgtg ataacttctc
    15421 ctgtacctga aacaagaagc atttctttat cattcattac gatcagcatc gcttcaagtt
    15481 tttgcataga ttgctgtgtc cgccattctt gagctaattc gacagctgcg cgttgtagat
    15541 tcccgttata ttcgtttagt ttgccttcaa atttttcttc taaagtaaac gcatctgcaa
    15601 cacttccggc gaaacctaca acaacttctc cattgtagat acgtcgtact ttctttgcag
    15661 tgcctttcat gacaacagat tcacccattg ttacttggcc atctccagcc atagcaaatt
    15721 tcccgtcttt ttcgacggca cagatagtgg ttgaatgaaa tggtgattcg accatgtttg
    15781 tttcctccta aatttgttct tttccttatt tttacgctct tggatggaaa gaacgatagt
    15841 ttttttgtaa actttctttt gttacatggg catagatctg ggtagtcgat aaattggcat
    15901 gtcctagcaa ttcttggact gtccgcaaat ctgccccatt atttaataaa tgagtagcaa
    15961 atgtatggcg cagcatgtga ggatggatat cactatccaa actgcttttt ttgatcattt
    16021 gattcaagac atattcaatt ccagtaggcg tgattggttt tccatgatga ttcacaaaaa
    16081 cgatatcatg ctcttcgtga tattttgtca tcaatacctg gcggccttcc ctgaaatatt
    16141 cttgcagagc atcttgagca aaagaaccta gaggagcata tctctctttg ttccctttac
    16201 catggatcaa catgacctct gaatcaaaat caatgtctga taccgacaaa ctgctacact
    16261 cacttaaacg gatgcctgac ccgtaaagga cttccagtaa agcgcggttc ctcaaatcca
    16321 aaggactatc tcctgctacg ctgtcaaaaa gtgcttgcat ttcattttca taaaaaaaac
    16381 gtggcagtct caaatttttc tttttgagat gaacgtaaga aaatggattt tcttttatca
    16441 cttcttcttt gagaagatac tgataaaacg aacgtaggct ggctatcttg cgactgatag
    16501 aatttctgct atagctagga cgatgatcag ctaatgcact caaatagaca cgaaccgtaa
    16561 aatattccgc cttcagcaga tcagcgtctc cagattcctc caaaaaatgt ttgaaatgga
    16621 gaatgtcttc ttcatatgct tctttcgttt tttctgaata tcctctttcg acaatcaaat
    16681 aacgcaaaaa tttttgttcc cacgtggctt ctgtcatttt ctcaccttcc ttttgtccca
    16741 gagaataatt tagcacagcg tttataaaaa gacaaataaa ttgtcagaat ttaaccaata
    16801 tttctaagtt tgttcacaat tggcgcaatt cctcacattt tgtttgagaa attcttaaga
    16861 aaaacgcaag aaaaagagcg agactatccg tctcaccctc ttcatggagt ttccttactc
    16921 atttgggatt gcagtttttt ctaattctgt ttttattgcc tcattagctt ttaaagctcg
    16981 ttgtgcaatt gcttcatatc ttgctttttt gtctcgaatg cgctctggca gttcaggaaa
    17041 cagaccatag ttggcattca tcggttggaa atgttttcct tccgcatgag taatgtagta
    17101 agccatactt ccaatagctg tttcttgtgg aaactcgact ggttccattt cttttgctaa
    17161 gcgtgcggcg ttgattccag caagtagtcc gctcgctgca ctttcaacat agccttctac
    17221 accagtcatc tgacctgcaa agaacagatc ttcgcgtttc gttgattggt aagtcggttt
    17281 cagcaattct ggcgaattca taaagctgtt tcggtgcatc acaccatagc gaacgaattc
    17341 agcattttct aacccaggaa tcatccggaa aacacgtttt tgctcgcccc atttcaagtg
    17401 agtctgaaag cccacgatgt tgtataaaga agctgcagca ttgtcttgac gcagctggat
    17461 aacagcataa ggacgtttgc cagttttagg atcttccaat ccaactggtt tcataggtcc
    17521 gaaaagcatc gttttgaatc ctcgctgtgc cattacttca attggcatac atccttcaaa
    17581 atatttttct ttctcaaaag atttaagttc tgccacttcg gcattgatta gtgcctcatg
    17641 gaaagctttg aattcctctt ctgtcatcgg acagttcaag taagctgctt ctcctttgtc
    17701 atagcgggac ttcaaatata ctttatccat atcgatcgta gctttgtcta cgattggtgc
    17761 agctgcatca tagaaataaa aaccatctga accattgaat gctttgatgc tttctgctaa
    17821 tggatgagaa gtcaaaggtc ctgttgctac tacggtgatt ccttccggta tttcagtaat
    17881 ttcttcgttt atcactgtta ctaatggatg ctctttgact tttcgggtaa ttgtttcaga
    17941 aaaagaatcg cggtcaacag ctaaggcacc accagctggc actgctgttt catctgccga
    18001 agtgattact accgaattca atcggcgcat ttcttctttc aataacccta ctgcattagc
    18061 caaactgttt cctcttaatg aattggaaca tactagttca gcaaaattac ttgtgtgatg
    18121 cgcctcagtc gatttgactg gacgcatttc atataaacgg acagggactc ctgcttgtgc
    18181 gatctgccaa gcagcttcac ttccggcaag ccctgctcca acaacattta ccattttcaa
    18241 tgaaagaacc tcttttctat tatttttgga tgtcttcttc gtaatctcca tttacacaga
    18301 ctacttgttt accgcctttg actttcttct cgaccagata tccgtcacat tttggacaat
    18361 ttctgcccac tggtttatcc caagaagtga attcacattc tgggaaacgg gaacaaccat
    18421 agaatagtct gtttttctta gattttcgtt caatgacttg tccttcatga catactgggc
    18481 aagtcacacc gatttctttt acgattggtt tcgtattccg acaatcagga aaattactgc
    18541 aagcataaaa cttcccatat ttccctaatt ttatcaccat cggatgtcca cagacatcac
    18601 aatcgaatcc tgctggttca tctttgattt gaattttttc gattttttct tccgcattcg
    18661 tcagttcttt ttcaaacggt ttatagaaac gatcaaccac tttgacccac gcttccgtgc
    18721 cttcttcaat tttatccaaa tctccttcca tttctgctgt gaaatggata tcgacgattt
    18781 gagggaagaa ttcacagatt aatgaattga caatttctcc tagttcagtc ggctcaaatc
    18841 ttttttgtgt caattttaca tagtagcgtc tttggatcgt atcaagtgtt ggtgcataag
    18901 tggaaggtcg ccctaccccg ttttcttcta gtgcacgaat caacgtagcc tcactaaaac
    18961 gtgctggtgg ttgagtaaaa tgttgttttg gttcaatatc aacagattgg actacatcgc
    19021 cttcttcaag ttctggaaga atattttctt tctcttcttt gccgtcatct ctaccttcca
    19081 catacacttg catgaatcct ttgaatttga tttttgaacc gttcgcaatg aagatgacac
    19141 cattttgttc taatgtcact ttcattgtat ctaaaatagc tggtgtcatc tgactagcca
    19201 caaaacgtga ccagatcaat gtataaagtt tcaattgatc tttatctaaa tattgtttga
    19261 gttcatctgg tgtacgaagc acactcgttg gacgcacagc ttcatgggca tcttgtgcgc
    19321 cttgagcatt tttgatcttt cggtgaccat gtctagaaaa ttcttttcca tacgtatctt
    19381 caataaatgc tgcggcctct gcttttgctg tatcggcaat ccgggtagaa tcagtacgca
    19441 tgtatgtaat caagccgact gttccttgtt tacccaaagt gattccttca tataattgct
    19501 gtgctaccat catggttttt cttgttcgaa agtttaactt acgggcagct tcttgttgca
    19561 ggctacttgt agtgaaagga ttcgcagggt ttcttttgcg ctctttcttt tctacttttt
    19621 taacttcgaa gtctgttcca tcaattctag cagtgacttc ttttacactt tgtgcatctg
    19681 gtaatttctt tttcttaccg tcgattcccc agaaatttgc tttgaatttc ttgcgagctt
    19741 ttttaaaatt accgtcgatg ctccaatatt cttctggaac aaattctcgg atctcttttt
    19801 cacggtcaat aataattttt aaagcgacag attggacacg tcctgcactt aaccctttct
    19861 tgaccttacg ccagagaata ggactgatcg aataaccaac gattctgtct aaggtacgac
    19921 gagcttgctg tgcatctact aaatctacat cgatcgttct tggttcctta aaagctgctt
    19981 tgactgcgtc ttttgtgatt tcattgaaga ccacccgatt tttttctttc agatccaacc
    20041 caagtaggta cgctaaatgc caagcaatcg cttctccttc tctatccggg tcacttgcca
    20101 agtaaacttt ttgagctttt tttgctgcgg ctttcaggct tttgatgaca tcgccttttc
    20161 cgcgtataga aatataatgg ggttcataat tgttttcaaa atcgataccc attttacttt
    20221 tcggtaaatc tcttatatgg ccaacactgg ccattacttt atagtttcgt ccaagatatt
    20281 tctcaattgt cttggctttt gcaggtgatt ctacaatcac taaatattta tacgccatta
    20341 ctgaatggac tcctttccat tgtcgtgttc ctactattaa aagaggattc atacaaatcg
    20401 tagctaatca tatccattca gaaaacgttt gtcaagaaaa gcaaatgaaa agtatggtgt
    20461 tattctttaa attctgccaa gatatcttca cccgaaaccg tgcaaactgc accgtcttgg
    20521 atcaaatgat gacatccggt agaatgttct gttaaaatat ttcctggaac agaaaaaact
    20581 gttcttccaa gatccaatgc ttgctgtgct gttatcagag agccgctttt tttcttggct
    20641 tcaaagacac atgttccttt agacaaagct gcgataatcc gatttctcat tggaaaacaa
    20701 tgctttttcg ggccggattg aggaggatat tcactcaaaa gaagatgatc atttgcaatc
    20761 acctcttgca agtcacgatt ttcagcagga tagacttgct ctatccctgt tccaatgact
    20821 gcgattgtcc gtccaccatg agagattgct gcttggtgac cgtatgtatc tatccctttc
    20881 gcgcagccac tgacgatcac atatccagct tctatcactg gttttagtaa ataataaact
    20941 gtttttttgg ctaaagcaga agtgtgtctt ccaccaacaa cgcttaacat gttacaagaa
    21001 agtaaagata gatcaccatt ataaaacagt acaattggcg gataggtcaa gtgaagcagc
    21061 tgagggggat atttgggatc aaagcaagtg ataaacgact gtttcgattg gattttgccc
    21121 cagttagcgt gaatttcctg ccaacttttg cgaaacagct cccggtgttt ggttatttta
    21181 cctatcttaa tgatgtcttc aaatccgatt tctttataag gatactgcat catccagtta
    21241 gcaagtgccc attttccttg actactgatt ccttttgtat aagcaagttt caataaattt
    21301 tcttctattt gatacatcat aaaaccgcct ttcgtctctc tttctaataa gatacgtaaa
    21361 agcggctgtt ttttattgta aatatttttt tacaggtgca aaacttttac gatggattgg
    21421 cgtaattcct tgtcgttcaa gtccttctaa gtgttccttt gttccataac ctgcattatg
    21481 cgagaaacca tatccaggat aaagcacgtc atattctttc atcaattggt ctcgatatac
    21541 tttagcaatg atacttgcag cagcaatcga cacagatcgt gcatcccctt tgattatttt
    21601 ttcctgctga ataggcaaat ccaaggtcat cgcatcgatc aacaaatagc ttggtacaat
    21661 ggacaatttt tctactgctt ggatcatcgc taatttactt gcttcataaa tattcacatc
    21721 atcgattgtc tgctgatcaa cgatcccgat cccgattgcc agggcttttt cttgaacttg
    21781 ttgataaaga agttctcgct ttttctctga taattgtttg gagtcattca gccctaatat
    21841 gatctcattt ttcggcaaga taactgcagc tgccacgaca ggtccagcta atggtcctct
    21901 tcctacttca tcaatgccgg caatccattg gtgaccttgt gcgtaccctt gcttttcaaa
    21961 gcaaaacatt tcttcatatt tttccttaat taatgcttcc tttttcaatc gtcgttcaaa
    22021 agaagccagt gcttgctgca cgcctttgcg tggatcattt cttaatgatt ctagtgtagg
    22081 atcatctatt ctctgtatgc ttttcaatcg ttctttgata ctttggatgg attcagtcat
    22141 cacttttttg ctccatttct tcgcaacgat ccagtgtgta tcgtccaagt tttccttgac
    22201 gaatatcatg cacgactaat tcgctgccac gatcatagtc ttctttatac ccacgttttg
    22261 ctgtaatatc catcagtaat tccggtgcca atttttcttc ttgctcgatt gataaaccat
    22321 aacgatcgct gattcttcct gaataatatc gggcaaaaaa gtctaatccg taaatcgcta
    22381 aatcatctaa atggacaagc tggtctttga tcgcgcctgt cagcgctagt tttttgccga
    22441 tttcttcatc ttcaaatttg ggccataaaa tccctggtgt gtctaacaat tccaactcat
    22501 tacctaaacg aagccactgc tgaccttttg ttacgccagg cttatttcct gtctgtgcaa
    22561 ttttcttgcc tactagtcga ttcatcaacg tagatttccc aacatttgga atacctaaga
    22621 ccattgcccg aatagcgcga ggcttgatcc cccgagcagc atctcttgcc cgtttttctt
    22681 tcaatgcttc tttagccgca ggtactagac ctttaattcc tttactttct tgtgccgtga
    22741 tagctaatgc aagatggcct tgtttacgaa aatattcttg ccacagcttt gtctgttgtg
    22801 gatctgccag atccgcttta ttcaataaaa caaggcgagg tttttgctga atgatctgat
    22861 ctagcattgg attacgagaa gataaaggta atcgtgcgtc taccaattca aatacgatgt
    22921 ccacatattt cattttttca gaaacttctc gtcgggcttt tgccatatgt cccggaaacc
    22981 attggatggt cattgttctc tccattcctt tcataccgga atattccccg gctttacttg
    23041 tttttttctt tttcaatata aattcacttt tttattatct taattgtcaa attaagttag
    23101 acaaatcatc aatacttacg taactcaaaa atacttccaa aggtgttcga taattcagtg
    23161 attttctagg aatattattt cgtttcgatg cgacagattg aataaaa
//
LOCUS       DAZUMK010000034        22532 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000034 DAZUMK010000000
VERSION     DAZUMK010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.034"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..229
                     /locus_tag="ACG808_001277"
     CDS             <1..229
                     /locus_tag="ACG808_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFS5046911.1"
                     /translation="NLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVAAGK
                     TAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            222..923
                     /locus_tag="ACG808_001278"
     CDS             222..923
                     /locus_tag="ACG808_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFS5046912.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            923..2509
                     /locus_tag="ACG808_001279"
     CDS             923..2509
                     /locus_tag="ACG808_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5046913.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            2528..2749
                     /locus_tag="ACG808_001280"
     CDS             2528..2749
                     /locus_tag="ACG808_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFS5046914.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            2746..3507
                     /locus_tag="ACG808_001281"
     CDS             2746..3507
                     /locus_tag="ACG808_001281"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFS5046915.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            3887..4396
                     /locus_tag="ACG808_001282"
     CDS             3887..4396
                     /locus_tag="ACG808_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFS5046916.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(4471..5010)
                     /locus_tag="ACG808_001283"
     CDS             complement(4471..5010)
                     /locus_tag="ACG808_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFS5046917.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(5150..5761)
                     /gene="rpsD"
                     /locus_tag="ACG808_001284"
     CDS             complement(5150..5761)
                     /gene="rpsD"
                     /locus_tag="ACG808_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFS5046918.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            6023..6874
                     /locus_tag="ACG808_001285"
     CDS             6023..6874
                     /locus_tag="ACG808_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFS5046919.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            6902..7537
                     /locus_tag="ACG808_001286"
     CDS             6902..7537
                     /locus_tag="ACG808_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFS5046920.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            7557..8330
                     /locus_tag="ACG808_001287"
     CDS             7557..8330
                     /locus_tag="ACG808_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5046921.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            8738..9535
                     /locus_tag="ACG808_001288"
     CDS             8738..9535
                     /locus_tag="ACG808_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFS5046922.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            9513..9926
                     /locus_tag="ACG808_001289"
     CDS             9513..9926
                     /locus_tag="ACG808_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFS5046923.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            9910..12555
                     /gene="mutS"
                     /locus_tag="ACG808_001290"
     CDS             9910..12555
                     /gene="mutS"
                     /locus_tag="ACG808_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFS5046924.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            12573..14681
                     /gene="mutL"
                     /locus_tag="ACG808_001291"
     CDS             12573..14681
                     /gene="mutL"
                     /locus_tag="ACG808_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFS5046925.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            14703..15263
                     /locus_tag="ACG808_001292"
     CDS             14703..15263
                     /locus_tag="ACG808_001292"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFS5046926.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(15348..17336)
                     /gene="treP"
                     /locus_tag="ACG808_001293"
     CDS             complement(15348..17336)
                     /gene="treP"
                     /locus_tag="ACG808_001293"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFS5046927.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            17559..18272
                     /gene="treR"
                     /locus_tag="ACG808_001294"
     CDS             17559..18272
                     /gene="treR"
                     /locus_tag="ACG808_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFS5046928.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            18349..18795
                     /gene="msrB"
                     /locus_tag="ACG808_001295"
     CDS             18349..18795
                     /gene="msrB"
                     /locus_tag="ACG808_001295"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFS5046929.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            18965..19567
                     /gene="ruvA"
                     /locus_tag="ACG808_001296"
     CDS             18965..19567
                     /gene="ruvA"
                     /locus_tag="ACG808_001296"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFS5046930.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            19580..20581
                     /gene="ruvB"
                     /locus_tag="ACG808_001297"
     CDS             19580..20581
                     /gene="ruvB"
                     /locus_tag="ACG808_001297"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFS5046931.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            20610..21188
                     /locus_tag="ACG808_001298"
     CDS             20610..21188
                     /locus_tag="ACG808_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5046932.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            21240..21722
                     /locus_tag="ACG808_001299"
     CDS             21240..21722
                     /locus_tag="ACG808_001299"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFS5046933.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            21839..22213
                     /locus_tag="ACG808_001300"
     CDS             21839..22213
                     /locus_tag="ACG808_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046934.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7342 a         4046 c         4883 g         6261 t
ORIGIN      
        1 taatctagcc attttatttg ttcctgctgg tgtaggttta ttggaatatt ttgatgatat
       61 tgcttcgata tggcctgttt tactgggagc agtcgtcgtg tgttccatcg tcacgatggt
      121 tgcagcggga aaaacggcag aaggtgtaga agctttgctg ggctatgtcc gtaagaaaaa
      181 agcagaaagg gtatctgtaa acgaaatgga ggagaatcaa attgactgag ttttttgtga
      241 atccattatt tggcttaagt ttgactttag gagtgtacgt attatctgac aaatttttgc
      301 ggaaatataa actgcctgtt ttgaatcctc tcgtattatc gattgttgtt attatcttat
      361 ttttgcattt ttctgggatc agctataaag actataatat cgggggcaat tttttgacta
      421 tgctgattac tcctgcgaca gtggcacttg ccatccctct ttataaaaac ttccgtttac
      481 tgaaagagaa tttttatcct gtaatggctg cgattttagc aggaatagtc gcaaattgct
      541 tggttagtgt agggttcggt tacttcttct ctcttcataa agagatggtg atctcattac
      601 tacctaaatc tgtaacgacg gctatttctg tcgatttatc tcatacgatg ggcggggtga
      661 atgccgtgac acttgctatt gttgtctcta caggtatttt tggttcgctg atcgccactc
      721 atattttccg cctattcaag atcgaaagtc cagttgctcg cggtgtggcg ttaggaagta
      781 cttctcatgc tattggtaca gcaaaagcaa tcgagattgg tgaaatagaa ggaatcattt
      841 ccggacttgc gatatgcgtc aacggaatat tgaccgtttt attgctaccg ttattctttc
      901 agccttttgc tggcttgttt tgatgcgaaa accactattt tatgctacaa taaccaaaac
      961 gttcgagcat aaaaaaggag ggaaagacgt gtatttgtct tccgagtatc ttaaacaatt
     1021 tgataaagat ctatattata agattctcct cctagaaagt tttgaggatc aagcttggca
     1081 taccgctgca caattagcgc aagttgttca gctggatgcc cgcagcgtgt cgaaatacct
     1141 gaacgagctg tctaaaaact atcagcagtt tagcggtaag acacaccctt tgtttacaaa
     1201 aaatcaccgt tctggttata acttttatga tacgctcgat tcaattgaac atgagcgttt
     1261 tttgatctat ctcgtgcaaa gtacgctgaa attccagttg ctgcacgaca ttttttttga
     1321 agaattccat acaatgtacc aatttgcgca aaagcattac attagtgaat ctactgctca
     1381 tcgaaaaatc aatgaatgga aacagcaact gcaaacttac ggaattcggc tgcaacgagg
     1441 tacatatatc gctcaaggcg aagaagaaat cattcgactt tacttgcata tgacattctg
     1501 gcagttgttt cgagggaaaa tctggccatt tgaaacgatt tctcaaatgg acgtaaagaa
     1561 tatggcagaa catatcatgg ctttttttaa tgttcgtctg aacgagatca aaaaaagacg
     1621 attggaatat atgctaggtg cctttttttt aagaaaatca cagaagcatt atgttgtatt
     1681 gaatgagaaa aaaagacggc tgatttctga caatctgttg ttccagcgat tttgccaggt
     1741 catggagccg gtatttccta attattttca agtagaagac gagttaggcg cattatttct
     1801 tgtactgatg actcgtgaag aatattatag tgatcctaag atacggaaga aaatttttga
     1861 ttttcatcaa caagcaaaaa caccgccctt tacagccctt tcagaagcaa aagccgctct
     1921 ttctctttat caggaagagc aggggctgcc agcagaaaat ctaaccttcg aggcagaaaa
     1981 ctatttattc agcagtcatt ttttcgccta tctttttcct aatgcgaaag aaacgattga
     2041 tggcaatagt agtgatttta ttaatcattt agttattgaa aacaaggaat tgaaacaatg
     2101 gctcgtacat ttttttgaaa gtagacacaa acatcccaat catctggcat ttaaaaatca
     2161 tgcgtttttg atggggcggt atctgactgt gtttaaaaca ttaggagcct ttactccaca
     2221 gcttccaaag atcacgatcc ttttaatgac agatttccca ttatttgaag agcaactgtt
     2281 agaggaggga ttacggacat ttttccgtaa tgagtatcag ctgatttttc tgccaacaga
     2341 ttatcggggg agagaagttg atcttctgat ttcgacaagt aaagtccacc gcaaaccatg
     2401 ggcggatctt gactatttta ttgtgactga agaactaaaa ttaattgatt acatccaact
     2461 atctcaaaaa tttgaaatga ttcaaaaaca aaaactatta aaacaataac aaagaaggag
     2521 aaaaaagatg acatttgaag aaattttacc agaattgaaa aaaggtgcaa agattattcg
     2581 taaaggatgg agtggatttg aactttatgt tgttctcgta tccgatgatg aatttgatgg
     2641 ctgtccggtg acgccgtact tattgatcaa aacaagtgac gaaggttttt ccagcttcgc
     2701 tcctacagtt tgtgatattt tagcagatga ttgggcgatc gtagaatgaa aaaccagtat
     2761 ccagatctag ctaaacaggt tgtatttatc acaggagctg cttctggaat tggtgctgcg
     2821 caagcccgag cgtttcttga acaagatgct tttgtatttg gattagatca aaagttcggt
     2881 aaaatggaag aaatttcttt gcgatttcct gaaacttttg cctacgcact aggtgatgtt
     2941 cgacaaatga aagaactcca acaagcggta tcgaaatgcc aaaatcattt tggtgaagtt
     3001 acgattttac tgaatacagc ggggatattg gatgcctaca aacccttgat ggaaactgac
     3061 gaatcgctgt gggatctgat ttatgagacg aatgtgaaaa gcatgtatca gctgacaaaa
     3121 atcatccttc ctgatatgat ccgccaaaaa agcggaacga tcatcaatat ggcttcgatt
     3181 gctggcttga ttgctggtgg aggtgggatc gcttatacaa gcgcgaaaca tgcgattgtc
     3241 ggatttacca aacaactcgc tctggattac gcaggacagg gtatccgagt aaaaggaatt
     3301 gctccaggag ccatccaaac accaatgaat gcggctgatt ttgaagggaa aggtgagatg
     3361 gcagagtggg tggcaaatga aacaccagtc aagcgatggg cacagccgga agaagtagct
     3421 gagctgacgt tatttcttgc ctctcctcaa gcaagctaca tacaaggaac gatcatccca
     3481 atcgacggcg gatggttgtt aaaataacct tgtgtttttt tcaagcttct gtatgataaa
     3541 actaaagaga gaaacaacgg ttgatgataa aaaaagtcga tgactgttcc cgacattcga
     3601 tgccgaacag ccagttcaaa aaattctttt taataatcgt taatattcga aactaagcgg
     3661 cttttcacga cctcttgata taaacgatat tcaaaagctt gcaactgcgg taacaagtcc
     3721 agccaaatga aaaatataag gagctatttt tatttgacca ttcttattac ttgttgcagg
     3781 gcagcttttt cactaccttc tgcgacactt cagttggaat tttcttccca tcctttggtc
     3841 gcggtcatta ccaagataaa attatcttgc aaaggagaaa tagaacatgg aaaaaaataa
     3901 tacccgttca gccaaacgct ggacaacgac agatgtgact cggatggcgc tagtcactgg
     3961 tttatatgtg gcagtgacgc tggttttgtc tgtctttagc tttggagtga ttcagattcg
     4021 tctctcggag atgttcaact atctgtcctt gtataacaaa agatacatcg tcgcggtaac
     4081 agcgggagta gctttggcta atattgcttc tcctcttgga ctgatagatg ttgcagtagg
     4141 aagcgtcagc acgctgcttg tattgcttat caactataaa atcactagtc gcatcaaaaa
     4201 catgaaaatc aaaatggccg tgacagcatg cgtatttgct ttttccatgt ttacagtagc
     4261 tggtcagcta acgattttat atcaactacc ttttttcttg aattggtggg tgattgcttt
     4321 aggagaattg gcatcaatga ttgtcggtgg gataattatt tattggatcg gtaaaaaagt
     4381 ggatttgtca aaataaagat ctgtttaatt taaaataggg cagcatggaa gagactgaat
     4441 gttgagtctt cttccatgct gcttttttga ttaatttgta tgaagaaatc gatttagccg
     4501 ttttttcatc aagataccga tataagctgc tccagttgcc ttgattgctt ccggcagtaa
     4561 aaacggcagt agtccactgg caaatgcagt tgtaaaagta agatcaccac ttattttcag
     4621 ccagatcgtt ccgcagatca atgcagcaag agagcccatt agattggcaa agattgcata
     4681 taagtaatga gcatccgttc tttcgatcat ccaaccagtt atccacgttt gtaagagaaa
     4741 tccaatcaaa tatccgccag ttggcccaaa taaaacagct atacctccag tcatccctga
     4801 aaatacagga agaccaatca agccaagtaa aagataaact ccaacagata tcaatcctag
     4861 ttttcttccg agaaaagttg ctgttaatcc aatagcaaat gtttgccctg tcaaaggaac
     4921 caaaccaaaa ggaatggtca tctgagaaag gacagaaata atcgcggcaa attccgcagc
     4981 aagtatcaat tctttcaatg ttcggttcat aacgccctct tttcgttaac ttaatatttt
     5041 tatttggtta acgaaattgt aaaacaaagt gattactttg taaatagaaa atataaaaaa
     5101 agaccagcta tccaatagat agcggtcttt ttcaagaaag cttttgaaat tataagcttt
     5161 ggttgtagta ttcaacgaca agagcttcgt cgatttctgg atatagttcg tcacgttctg
     5221 gtaaacgagt caatgaacct tccaattttt cagcgtcaaa gcttacgaat gctgggcgtc
     5281 caacagttgc ttctacagct tcttttactg ttacgatgtt ttgtgatttt tcacgaactg
     5341 agatcacttg acccacttct acgtggtatg aagggatatc aacgcgtttg ccatcaacta
     5401 aaatgtgacc atggttaact aattgacgtg cttgacgacg agtcgttgca agtcctaaac
     5461 ggtaaacaac attgtctaaa cgttgttcta gtaagatcat gaagttaaca ccatgtttac
     5521 cttctttgat tttgctagct tttacgaaca agttacggaa ttgacgttcg ttcattccgt
     5581 acatgtgacg aagtttttgt ttttcagtta attgcatgcc gtattctgat tgtttgccac
     5641 ggctattagg tccatgttga cctggtgcgt aagggcgacg tgctaattct tttcctgtgc
     5701 ctgacaatga gatgcctaag cgacgagaaa ttttccatga tggtcctgta taacgagaca
     5761 ttaaaatttc ctccaatgaa tataagtttt tggagtaaaa taatccgttg aaaaattcat
     5821 aatcatatag tttgttcttc aatcttcacc tttgcagccg cggttacaca atcgaacccg
     5881 agacggggca acaaactagg gatgagttat tatttttctg ctgcattatt ttacacaaat
     5941 tacagtatat gatagatcat ccacgaatgt caagataatt agtacataaa aacgctttac
     6001 aaacctaggc tgtctttata aaatgagagg gtatgtgcaa atggaaaaaa gcgaaggaag
     6061 tttaaggaga aaatcaatga atccggtatc accattattt gagaaaatcg atgcttcaat
     6121 tgaaaagaag gcgaatcttt ttgaaaacag cttttcacgt tacgctgttc gagcggttct
     6181 cgcctgtttg tttttgactt taggaacggc tgtggcattt gcgatcgcga tgaaggggga
     6241 agatatcgcg catggtctag gaaagatttt atacgcattt atgttcagct ggggtctggt
     6301 catgattcta tacatgaacg ccgaattagg aacatcgaat atgctttata tgaccgtggg
     6361 tgtttttcgg cagagacttg ctttaagtaa ggcagccaag attctatttg cctgtatttt
     6421 tttcaatatg attggcggta tattatgtgg atttctaatt tctatgacta cgccatttca
     6481 agatttaacg ttggacaact ttatgttaga atcaatagca gtaaaactag caaaaccttc
     6541 agcaacgatt ttggtggaag ggatgtttgc gaatatcgtt gtcaataccg ctgtactgat
     6601 cagcatgcgg atgaaagagg atgtcggaaa agtgatcaca gtcgtattca tcattttcat
     6661 ttttgctttt ttaggatatg aacacgtgat agcgaatttt cctgccttta cgcttactta
     6721 ctttgcttct cacggacaga tggatggaat gacagtcagc aatgtgttac ataatttgtt
     6781 ttttgctttg gcaggaaatt atattggtgg cggtttagta atgggactgg gatatgcttg
     6841 gctgaatcag tcaaaatcat cctatgtgga ctaaaaagtc gttcattaaa ggggaagtgc
     6901 catggaagtt ttagtttttc caaaaaatga ggaaaaagcg ttacaaatgg aaaagtacat
     6961 gaaaaatctt ttttcctttg ctggagtacc taagccggaa cgcgctcttt tacaacaacc
     7021 gttactggat gagagcaaaa atttatctat ttctgaagtt ttagcttttg ttcattatta
     7081 ttatgaacaa atcgaaagag agtatcagta tgtagcgatc gatttagtcc agaaaaacat
     7141 caaacggctt acatttgaag acttagtcgt attgttgcca ctagttcaga aaaaagaatg
     7201 gtgggacagc atcgactctt ggagaaaagt atacagcaca tggtgtaaag cctacccaga
     7261 gcagcgtgaa gaagtctttc aattgtttga aaaaaaggaa gatttttggc tacgtcgaat
     7321 agcaatcacg ttgcaattag gttataaaca gcaaacagac acaggactat tgatccgagc
     7381 aatagacgaa gatcgtcaaa caaaggaatt ctttatccaa aaagcaattg gttgggcatt
     7441 acgcgattat agtaagaccg atgccgattg ggtaaaaaga cagctgacaa cagatttgag
     7501 taagttagct gttcgagagg caagtaagta tttataagaa agcgagacga caagagatga
     7561 gacgcaaatt atttgctttt gatattgatg gaacgttgtt agattcaaac aaacaagctt
     7621 tagatagcac aagagaagca ttggaaaaat tacgcaaagc cggtcatctg gtcacgattg
     7681 ctacagggcg gagccgattt catgcgcaag agatcattcg cgatcttgat tttacgaact
     7741 acattttatg taatggagcg gccggttttt tagaccatga acaagtatat aagaatttac
     7801 tagatcgcga ccagttggat gcgttcgttc aagaagcgca tcatgagcag atcgatacag
     7861 cttttgtcag cttagacaat atcaagcgtt cttcttccaa tgatgtaacg gtgatggaag
     7921 aagcgatgca ttctttcggt gctgtcttgc ctgaattaga tcagtttttt gcagaggaac
     7981 aagaaatata ccaagcgtta gcatttttcg atcaaacata tgatgggaag ttctcttcat
     8041 atgataaaat tcgatttgtc cgctggcatg agaacagtgt agatgtcgta cctaaaaatg
     8101 gttctaaagc agcgacaatt ctgcacttag cagaacaagt aggcatccct catgaagaca
     8161 tcatcagctt tggcgatgga caaaacgacc gtgaaatgtt gcgcatgtca ggaattggga
     8221 tagctatggg gaatgctgtg ccagaagtac aagcagaagc taagatggtc actgatacaa
     8281 acgatcagga tggaatttgg aaggcattga aagagttaag cgtgatttaa aagaggttgt
     8341 gaaaaagctg ttctgcatca agtaataagc ccagccaaac aaaaatagct cctcctattt
     8401 ttcgtttttc tgggcttatt accgcagatg caagctttcg aacaccgttt aaggaagagg
     8461 tcgtgacaga agtggtcagt ttcaagaaat aagaagggat ttacgaaaat tgcttctcaa
     8521 atttttgtaa aattacggct tatttctgaa gaagtagatg aaacaacgtt tattcgggaa
     8581 atgggactgt gacaagatgt ttgtcatagt cccatttttt tattttattt ttcaaaacta
     8641 agaaatacca ggaaagagtt ccttttgttt gaccttttct ttcttattga aaacaactat
     8701 aatatagagt gatgttaaaa gagaagagga atttagtgtg cgggtattgt ttattggtga
     8761 tgtagtaggt tcaatgggac gagagatgat tactgaatat cttcctagat tgaagaagaa
     8821 gtaccgtccg caagtaacga ttgtaaatgg tgagaatgcc gcttctggac gtggaatcac
     8881 agaaaaaatc tataaaaaat ttttgcaaga cggcgtagat gtcgtgacga tggggaatca
     8941 tacgtgggac aaccgcggaa tctttgagtt tgtaaatgaa gctaaaaaga tggttcgtcc
     9001 ggcaaatttt ccggaaggga cgcctggaca agggatggtc tttgtcaaag tgaaccaagt
     9061 cgaactggca gtgatcaaca tgcaggctcg ttcttttatg gtggacttgg atgatccttt
     9121 tcgtaaaatg aatgagctgg tagaagaagc cagaaaacgt acgccgatca tttttgtaga
     9181 ttttcatgga gaaacaacaa gtgaaaaaca agcaatgggc tggtttttag atgggaaagt
     9241 ttcagcagtg gtcggtacac atacacacgt tcagacaaat gatgcgcgaa ttttgccaag
     9301 aggaacagcg tatctgacag acgttgggat gacaggtccc tatgatggta ttttgggtat
     9361 gcgcagagaa cctgtaatcg aaaagtttct gactgcatta ccaaaacgtt ttgaagttgt
     9421 ggaacaaggc cgatcgattt taagcggctg tatcttggaa ttggatgata cgacaggtca
     9481 tgcaaaagaa atccagctga ttcagatcaa cgatgatcga ccgttcatgg agtagaaaga
     9541 gaggcagttt tttgcagcag gagccggaaa ttcaaaaaga agtaaggaaa ctgacaaaac
     9601 ttttaagaga gaatgaaacg atcattcgct acaaagaact tgaagaaaag atccagcaaa
     9661 accagtattt agctgagttg agagaaaaaa tcaaacaagc gcaaaaagat gctgttcatt
     9721 ttgcccacta tgacaaacca gcagcagaaa aagaagcaat caaacaagcg gatcaattca
     9781 tgcaagaatt tgatcagcat ccgcttgttg tagcttatag aaaacagctg ttggaagcag
     9841 atgatctgct tcatcattta acaactatga tacaagaaga aatcaatgga caaatagagg
     9901 aggaaaaaca tgcctcaaaa aactaaaaat actcctatga tggaacaata tctaagcatc
     9961 aaggcccaat accaagatgc ttttttgttc tatcgtttag gagactttta tgaactattt
    10021 tacgaagatg cgatcaaagc gtcacagctt ttggaattaa cgttgacgag cagaaatcga
    10081 aatgcagaag aacctattcc tatgtgcggt gtacctcacc atgcagcaca aggatatatc
    10141 gatgccttga ttgaaaaagg ctataaagta gcgatttgcg agcaggtaga agatccaaag
    10201 acgactaaag ggatggtcaa gcgcgaagtg atccagctag tcactcctgg tacagtcatg
    10261 gacagcaaag gattgtcggc aaaagacaac aattttttaa cagctgtagt tagtcaagga
    10321 aatgaatttg gctttgctta tgccgattta agtacgggag aattgaagac ggctcggttg
    10381 catgatgaag aagctgtttt aaatgaagcc tctgcattgc agacaaaaga actcgtatta
    10441 ggcagtgaga tcactgatac ccttcgtgaa cagctaagcg ggcgtttagg gctagtcttt
    10501 tctcaacaaa atgagatgga agaaaacgcg gaatttagct ttttgacaag cgaactagtc
    10561 gatccgctgg aaaaagaagc aaccggcaaa ctattgactt atttatcggt gacacaaaag
    10621 cggagtttag ctcatatcca aaaagcagta gaataccaac cagatcattt tttgaaaatg
    10681 gattattatt caaaattcaa cctagaatta agccaatcta ttcggactgg gaaaaagcac
    10741 ggcactcttc tatggttgtt agatgaaaca aaaacagcaa tgggtggccg tttactaaaa
    10801 caatggctag accgtccgct tatccaggca aaacaaatta aagcaagaca agaaatggtc
    10861 gcttctttgc tagacagcta ttttgaacgt atcgatttgc agtcagcatt aacgaaagtc
    10921 tatgatctgg aacgcctagc aggtcgagta gctttcggta atgtcaatgg gcgagacttg
    10981 atccagctga aaacttcttt agaacaagtg ccaatgatca gagggttgat tgaaggaatc
    11041 gatcaaggtc aatggcaaag tctgctttca gaaatgcagc cgatggacca tctcgtccaa
    11101 ttgatcgacc aagctatcca agatgatccg ccgttacaaa taacggaagg aaacatcatc
    11161 aaagatggat tcaatgagca gctcgatatg taccgttctg ctatgagaaa cggaaagaaa
    11221 tggttggcag aacttgaagc aaaagagcgt caagaaacag gaatcaagaa cttaaaaatc
    11281 ggatttaacc gcgttttcgg ttattatata gaaattacaa aagcaaatct tggaaatgca
    11341 gaactggaga agtatgaacg aaaacaaact ttagcaaatg cagagcgttt catcacacca
    11401 gaactaaaag aattggagac acaaatcctc gaagcagaag aaaaatctgt tgatttagaa
    11461 tatcagttgt ttttagctgt gagagaagaa gtcaaaaagg ctatccagcc gctacaagta
    11521 cttgcaaaag caatcagtgc agtagatgtg ctacaaagtt ttgcaacgat cagcgaacgc
    11581 tatcagtatg ttcggccaga acttgtcagt gacaagcatc agcttctgat caaagacgga
    11641 cgacatcctg tagtagaaaa agtattagga catcaggaat atatccctaa cagcgtagaa
    11701 atggcagaag acgaaatgat cttgctgatt acaggaccga atatgtctgg taaaagtacg
    11761 tatatgcgac agttggcatt aactgttctg atggcgcaaa tgggctgttt cgttccggca
    11821 aaacaagcag ttttacctat ttttgatcgg atttttaccc gaattggtgc aagtgacgac
    11881 ttaattgctg gacaaagtac ttttatggta gagatgatgg aagccaatca agccctgcgt
    11941 tatgctacgc caaacagttt gattttgttt gacgaactag ggcgaggaac agcaacctat
    12001 gacggaatgg cgttggccca agcaatcatc gaatatatcc atcgtcatgt acaagcaaaa
    12061 acactctttt ctactcatta tcatgaactg acggtacttg agaaagaact tccgcagctt
    12121 aaaaatgtcc atgttggagc cgttgaaaaa gacggagaag ttgtcttctt gcataaaatg
    12181 atggatggac ctgcggataa aagctacggc atacatgtgg caaagatcgc aggattgcct
    12241 tccaatctac tagaacgagc agcagatatc ctgcatacgc tagaaaatgg cgataacgga
    12301 caccatactg ttccagctga agtagaagag aaaactaaaa cagccaaaca ggagcaaata
    12361 aaaccacctg cagaaaaagt aaatcaagac catatacaag accgtgtaaa agaagaaaac
    12421 caacagcttt ctttattcag tgaattatcc gaaaatgaaa cagaagtcat tgaatcactg
    12481 aagcaaataa atttactaga aatgacgcca atggatgcac taaacacatt atatgaatta
    12541 caaaaacaac tgtaggaaga aggcggatat cgatggccaa aatccaagaa ttatctgaac
    12601 gtttagctaa tcagatcgct gcgggagaag tagtggagcg accagcttcc gttgtgaaag
    12661 aattagtgga aaatgcaatt gacgccggca gtactcagat tgatatctta gttgaagaag
    12721 cgggactgcg gacgatccaa gtgattgata acggagaagg cattttggca gacgacgtag
    12781 agaatgcctt caagcggcat gcgacaagta agatccataa tcgcgacgat ctttttcgca
    12841 tacgaacgct gggctttcga ggggaagctt tgccaagtat tgcctctgtt tctgaaatga
    12901 ctctggaaac ggcgaatcag gaagaaaaac aaggcaccta tctgtcgctg aaaggcggag
    12961 aagttattga gcatcgacca gcaccactgc gtcaaggaac gaagattacc gtcagcaatc
    13021 tgtttttcaa tacgccagcc cgcttgaaat acgtgaaaac actgcacaca gaattagcga
    13081 acatcgggga catcgtcaat cgactggcat tgagtcatcc atcgattgct tttcgtttag
    13141 tacatgatgg aaacaaaatg ctggcaactg ctggaaatgg cgatctgaaa caaacaattg
    13201 cgggaatcta cggattatct accgcaaaaa aaatgctgaa aatcgaagga aaagatctag
    13261 attttgagtt atttggctat gtttcattgc ctgaggtgac tcgtgccagt aggaattact
    13321 tatcgacgat catcaatgga cgttttatca agaacttttc cttgaataaa gcaatcgttg
    13381 caggttatgg gtcaaaatta atggtcggtc gtttccctat tgctgtattg gaaatcaaaa
    13441 tggacccgct tttagtagat gtaaatgttc atccgacgaa gcaggaagtc cggctgtcga
    13501 aggaaaaaga actaacagag ctaattagta acgccattca aaaagcattg tctcaagaaa
    13561 atctgattcc aagtgcagcg gataatctac ggtttaagaa aaaagtacct gatcagccta
    13621 aaacagaaca aatcgaaatc gatttgtcct acgcttttga agaagaaaaa cctaaaaagc
    13681 caagtagttt gaattttgat cgagagacag gtaccttttt cgtagaagaa ctaaagaaca
    13741 catttgttga cagaattgtt gataagtctg tggaaaatgt tgaaaactat tctggttttt
    13801 ctgaagaaaa caccacttat ccagaaaaaa cagcgaaaac ggtggataag tttggtgaaa
    13861 atgttgataa ctttgtagaa aaaacagtgg aaaaatctga aaagaaagaa tttcccattg
    13921 tggataacat ggagaaaaca aatgaagaac aagcagataa atccgtagaa gaggatgaaa
    13981 agatttacga ggaagaattg acactccatc ctgaatttga tttcactcaa ggaaacgaca
    14041 aaacattaga acaagccatc aagaaattag aaacggaaaa accagtacaa cgttttcctc
    14101 agttggaata ttttggacag atgcacggaa cgtatctttt tgcccaaagt agcgatgggt
    14161 tgtacatcat tgatcagcat gctgcccaag agcgcattaa gtatgaatat ttccgggaaa
    14221 aaattgggga agtatctgat gatctgcaag aattgcttgt tccatttgtg ttagactatc
    14281 caaacagtga tgctttgaaa ttaaaagagc aaaaacactt gctagaagat gcagggattt
    14341 atttagaaga atttggtcaa aacagcttta ttgtacgggc ccatcctact tggtatccca
    14401 atggagaaga agaatcgatt attcgggaga tgatcgatat gctgctgaca actggaagtg
    14461 tgagtgtaaa aaaattccgt gaagcaacag caatcatgat gagttgcaaa cgatcgatta
    14521 aagccaacca ttatttgaat gaagcacaag cacgtgtgct gctgaaggat ctggcacgat
    14581 gcgaaaatcc tttcaattgt ccccatggcc gtccagtatt gattcacttt accaattcgg
    14641 atatggaaag gatgttcaaa cgaatccaag acccgcatta agtcaaagag aagagggaaa
    14701 acatgaagat tgtattagca tcccaatcac caagaagaaa agagttattg gctcatatcg
    14761 tttcagaatt cgagattgaa cctgctgata tcgatgaaac gccatttcca gacgaacagc
    14821 ccgtcgatta tgttcgtaga atggctgaag aaaaagcacg aactgtctgg gaacaagatg
    14881 aaaaagaaga ccatttagtg attgccagtg acacgactgt tgttctcaat caagagatca
    14941 tgggtaaacc agaagatctg gctgaggctg aattcatgct gaaaaaacta agcggggaaa
    15001 cacatactgt atatacagct gttgttttaa aaacaaagga acgagaagaa cggatactag
    15061 cagaagcaca tgtcactttt tatcctttga cagacgaaga gatcaaacgc tacttggaga
    15121 cgggggatta tgcggataag gcaggggcat acggcatcca aaatgctgcg agtgtattcg
    15181 tcaaagaaat ttctggagat tattacagta tcgtaggatt tccgattggt gctgtcaatc
    15241 aggcactgaa aacattcatt taaacaaaaa agcggactat ctgattttcg attgaaaatc
    15301 catgatagcc cgtttttttt attgaaaaga ttacttatct actctgacta agattttact
    15361 tcagctttac cgactgcttc gcccgggcga acaagtcctg attggtcaag agaaagtcct
    15421 gcaacttttt cattattggt caaagcaaca ataatgtcag ttttcttgcc agcttgttct
    15481 atttctggca aatccatttc agcaatcagg gtctctggtg tgacaaggta tccttctttc
    15541 acgaatacat tgaaaggaac accctttagt tcaacagtat ctaatcccat gtgaacaagt
    15601 acttccagcc cctcattaga aagaatacca atggcatgtt ttgtttcaaa gacactcgtc
    15661 actttaccgt ttactggtgc atagactttg ccgtttgacg gttcgacagc ataaccgtca
    15721 cccatcattt tttgtgagaa aactgggtca tttacttcag ttatttcctt gatggtccct
    15781 gcagcgactg cgaataatgt ttcttttgtt gcaactgccg taccagttgc tgaaacaggt
    15841 tcgaaagact gtggcgttgc tgttgcgcca tttgctgttt gaagttgtac atcagcagca
    15901 ttttcaggaa cagcaggatc aatcttattc aaaattccct gacgtctaaa gatgatcgtc
    15961 aagccaaatg gaatgatgac agcaatgatc attgcgatga tgaaaggtac ccatgtttct
    16021 gcttggatag ctagtattcc tggcaaaccg cctactccga tcgcgttagc tcgaacaccc
    16081 atcaagttag caaacatgcc agctaatcca gagccgacca ttgcagcgac gaaaggatag
    16141 acgtatttca aattgattcc gaacatagca ggttcagtta cgcctaagta acaagaaatc
    16201 attgctggaa tcgagatttg ttcttctttt ttattcccac gatgtaagaa gatgatggct
    16261 aagactgcag agccttgggc gatatttgat aaagcaatca tcggccataa attcgttgat
    16321 ccgaaatctg caatcagctg catatcgatg gcattggtca tatggtggag accagtaatc
    16381 actaaaggag catacaggaa tccgaatacg gcactaaaca accagctgat agaagacgtc
    16441 aatccagtat ttacgatatt tgagatccag cttccgactg tccagccgat aggacctaaa
    16501 ataacgtgtg cagctaaaac agttggtaac aatgaaaaca atggaacaaa aatcatcgag
    16561 atcgactgag ggatatattt gcggaagaag atttctagat acgctaacat aaaccctgcc
    16621 agcatggctg gaataacttg cgcttgataa ccaatcatct ggacttgagc aaaaccaaaa
    16681 tcccaaaatg gaatatctgc tgcggctgtg gaagctacac tataagcatt taataattgc
    16741 ggagaaacta acgtaattcc taaaacgatt cctaatattt gtgtagtacc catctttttt
    16801 gcaatactcc aagtgatacc tactggaaga aaatggaaaa ttgcttcacc cggtaaccac
    16861 aaaaaggcat tgacaccatt ccaaaacttc gagtgttcaa cgattgttcc accaagactt
    16921 tcaaactgga taccttcaag tacatttctg aatccaagga tcaatccacc gacaatgatt
    16981 gcaggaatca gtggtgtaaa gatttctgct aaaacggcaa tagcacgttg aacgggatga
    17041 aggttttgtt ttgcagctgc ttttccttgt tctttggaaa ccccttcaac tccagatact
    17101 gcaacgaagt cgttataaaa agtagaaaca tcgtttccta tgatgacttg gaactgtcct
    17161 gcattcgtga acatcccttt tacgctaggt atatcttcga tcgctttttc gtccgctttt
    17221 cctggatcat tcaggacaaa acgcatacga gtcgcacaat gactaactgc agcaatattt
    17281 tcttttccgc cgatatcatg taacagcttc tcggcatctg cttgatattt tgccatatca
    17341 ataaacccct ttctcttaac ttgtctatgc aagttgtttc ttcatttact atactttttt
    17401 tcggagagaa tcgcaagcgt tttattaaaa agtacgagag aaaatgatat cgttttaaaa
    17461 agcttaaaat aagcttttct taaaaatagg aacattgaaa tacaaaaaga gctgtagtat
    17521 agtgaagaaa tgatggaaga acgagtgagg gaaaaaacat gaataagttc aatgaaattt
    17581 ttttagattt ggaacagaaa attttagcga aagaatatcc tccgcattcg cttttaccca
    17641 gtgagaacca attgatcaaa atttatggtg tttcacgaga aacaattaga aaagcgctga
    17701 atcttttgac tacggaaggt tatattcaaa aaaaacaagg aaaaggttcg atcgtactgg
    17761 atcggaatcg cttcgacttt cctatttctg gattgactag ctataaagag ctacaggaga
    17821 cccaacgtat tccaagtgaa acgatcatcc atacgttaga agaaacagaa gtgacaaaag
    17881 cattgaacga gatcactggc tgggagatcg gtgcgcctgt ttggcatttg atacgcgaaa
    17941 gaaaaatcaa tggcgaagta gttatattag acaccgatta tctattaaag gaaatcgttc
    18001 ctattttaac tccagcacaa gcacaaggat cgatctatga atattttgaa aatgagcttt
    18061 ctttgacgat tgcttacgcg caaaaggaga tcacagtaga agaagtaacg gaaatcatga
    18121 aaacagcgat ggatcttcac gatgacactt atgcagtagt agtcagaagc ctcgttcatc
    18181 tggaagatac ccgctgtttc gagtattctg agtcggttca ccgattagat aaattccgtt
    18241 tcgttgagtt tgcccgacgc cgaaaagtgt aaatagttca aaagaaagca aattgttggc
    18301 atctgtccac tttggcgcta tgataggatc acaaaggggg catttttaat ggataaacca
    18361 ataagaccag aaaaagaaga actgaaagaa aagttatcac ccatggctta tgcagtcaca
    18421 caagaaaatg caacagaacg accatttagc gggaaatatg atgattttta cgaaaagggg
    18481 atctatgtag atatcgtcag cggtgaaccg ttattcagct ctgcagaaaa atatgatgca
    18541 ggttgtggct ggccatcatt cagcaaacca ataacgaaaa gacaagtgcg cgaaaaagcc
    18601 gatttttctc atggcatgca tcgaatagaa gttcgaagca aagaagcaga ttctcatctc
    18661 ggacatgtgt ttaccgatgg accagtagat caaggtggtc tacgttactg catcaattct
    18721 gcctcactga aattcattcc atatgaaaag atggatgaat taggttatgg ggaattcaaa
    18781 tcacttgtag aataatggaa atacacaaag agcggtacaa tacggggatt tactcgatcg
    18841 tccgctcttt ttttattcaa gcgattgcta tttgaaagaa acgctcccta gactcctttt
    18901 ttcacaaggt gaaatgtgct acaataaagg gaacgtatgt gtgcaaaaga aagaggacta
    18961 gaatatgtat gaatatctga caggtgtggt tacctttatc aacccctatt acatagtagt
    19021 agaaaccaat ggtatcggct accagatcgc actaggaaat ccttaccgct acagtagcaa
    19081 actaaatcaa aaaataaaaa tttatgtcca tcaggtaata cgtgaagatg cgcatcttct
    19141 gtatggattt gattcgctag aagaaaagca actgtttttg cgattagtaa gtgtttcagg
    19201 aatcggtccg aaaagtgccc ttgcaatcat ggcaagcgaa gaccactccg gtttgattca
    19261 agcagtagaa tccagtgatg tcacttattt gacgaaattt ccaggagtag ggaaaaagac
    19321 agcacaacaa atgatcttgg atctaaaagg aaaattcggt gagctgagta tcgatacacc
    19381 ttttagtcta tttgatgaag caaacacaaa agacgctaca gctctatctg aagcaatgga
    19441 agctctctcg gctcttggtt acagtgatcg agaaatcaaa cgtgtagaaa aacaattaaa
    19501 agaagtagat aatcagacaa cggatgagta tctacgccaa gcactcaagc taatgatgaa
    19561 aaaataagga ggagaccaaa tgacagatga atacttgtta tcaccagaat ctggcgaaaa
    19621 tgaactgagt ttggaaaaat ctcttcgacc acagctgctt tctcaatata tcggacagag
    19681 aaaagtcaaa caagaacttc agatttacat cgaagctgcc agaaatcggg aagaaccttt
    19741 agatcatgtt ctgttgtacg ggcctcctgg attaggaaaa acgacgatgg ccatggtcat
    19801 tgctaatgag atgaccgtaa atattcgtac gacgagtggc cctgctatcg aacgtccggg
    19861 tgacttagta gcaatcttga atgaactgga agcaggggat gtcctgttta tcgatgagat
    19921 ccatcgatta cctagagtgg tagaagagat gctttattca gctatggaag atttctatgt
    19981 agatatcatg gtaggacaag ggccaaccgc acatccggtt catttccctc ttcctccctt
    20041 tactttgatt ggcgcgacga cacgggcagg tatgctttct gcacctttac gagatcgatt
    20101 tggcatcatt tctcatatgg aatattatga cgaacaggat ctgcaggaga tcgttttacg
    20161 ttcttcggat atttttcaaa cagagatcat tgaagaaggg gcattggaaa ttgctcgccg
    20221 atcacgagga acgcctagaa tcgctaatcg cttgctcaaa cgggttcgtg attttgcgca
    20281 agtccaagga gacgggaaaa tcgatcgtcc tatagcggat caggcactta ctttgctaca
    20341 ggtcgatcag gctggattgg attatgttga ccagaaattg ttgcgaacaa tgatcgagtt
    20401 atatggcggc ggaccagtag ggttaagtac gctttcagta aacataggag aagagcgtga
    20461 gacggtagaa gatatgtatg aaccgtatct gatccaaaaa ggatttttaa agcgtacacc
    20521 tagagggcga attgctactg cttatgccta tgaacatttt ggttacgact atcatatatg
    20581 atttttgctt ttagattgga ggaaaatgaa tgagtgatac aacacgtacg aaacaattga
    20641 tcaaaaacgt attgattgat ttgtgccaaa cgcaatcttt tcgaaaaatc agcgtccaaa
    20701 aaatcgcaaa cgaagcaaaa atcaatcgac aaacatttta ttatcatttt acagataaat
    20761 acgatttgct tcgttgggtc tattgtgagg atgcattgca ttatttagat ccacaatgtc
    20821 tttcacttga taattgggaa gagcaagcat tgaaaatgct gaaagcaatt gctgagaaga
    20881 aagaatttta tgcaaatact gtgctttctg acccagaaat tttacaaaaa gagttttctc
    20941 aactgatcca aaaatcattt atcaagattt ttgaacaaat ggatgaagaa agacaacttt
    21001 ctgcaagaga caaactattt tatgctcgtt ttttttctta cggttgcagt ggtgtattag
    21061 tcaattggat cacgaatgaa tatgaagaaa caccgatgga aatcgctatg cagcttttcc
    21121 gtttagccaa ggatacggaa ttatattctt atcgtcttta tgcaaaagaa gagttggaag
    21181 aacagtaata gaaaaatttt ttgttggaaa agcaataatc gctaaaagga gatggggaaa
    21241 tgacaaaaat tttatttgtt tgtttgggaa atatttgccg ttcaccgatg gcagaaggct
    21301 tgctgagaaa acgaatagca ttagaaggaa gggaagcaga attttttgtg gattctgctg
    21361 ctacaagcac gtatgaagtt ggaaagacac cgcaccctgg aacgaaaaaa atattaaacc
    21421 aagaaaaagt agatatgacg aatatggttg ctcggcaaat cacaccccac gattttgaaa
    21481 cttttgattg gatcattggc atggatcaag aaaatgtaga agaactgaaa cgaagagcac
    21541 cacgctcagc gcaggggaaa atccatttgt ttttaagtag cattcctgga aaagaaaaag
    21601 aaaatgttcc cgacccatac tataccaata actttgatga gacatatcac ttgatcacag
    21661 aaggcctgaa tcattggtgg aatatttgga ttgatgaaaa taaaataaca aataatttgt
    21721 aaagttaacg ctttcttttt aaggcgatgt tagagcattt ccttgttttt ttgtaaaatg
    21781 ttaggtaaaa taaaattaat taagtaacac tttcttagct aaaggaggag tgcattcgat
    21841 gaacgaagag acagtatcgc caatgaagaa gcttacgaag ttagttactc atacttcgta
    21901 tcaacttttt gattcttttg taaatgacac tgaagaacat gctaagaatg tacgattgca
    21961 aggaaagttt attcttgagc tgcaaaaagc tgctactgaa catagtctag ttatcttaca
    22021 agtaagagca aatcgtttga gtagcaagta tgaaacgatc tcaggctggg tggcgacaaa
    22081 aacggtcgaa aaagaccgag tgatgatccg cctacaaaac gaagagcagc agatccggat
    22141 ggttcctgta gaacaaattg aaaaaataac aatcctttca ccagcagggg aacaggaacg
    22201 gttatcaaaa taatttatac cagatagaaa agcacgtatg agcttgtacc atcaatcatt
    22261 actaatcaaa aataaccgaa tgctttaagc tgatatcagt aaaacggaac gttttaggat
    22321 gagtatcata gcatgacagc attcggttga ttagttgaat cgattggatg gtacaagttt
    22381 ttttgataaa aaaaggtcct gctagccggg gagctagcag gataaggagt taaaaatgaa
    22441 aaagtgttgt tagggttgtt tgttggtatg cttatataat agtcattagt tgtgaatatt
    22501 ttgtgacgaa agttttcgaa agttgttaag tt
//
LOCUS       DAZUMK010000035        22456 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000035 DAZUMK010000000
VERSION     DAZUMK010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.035"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..267
                     /locus_tag="ACG808_001301"
     CDS             <1..267
                     /locus_tag="ACG808_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046935.1"
                     /translation="YSAFKKSDEYLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTL
                     GIHNSGYRMLEKRKCADYIPNETITKTDMALMNREAEKIINKLT"
     gene            complement(303..605)
                     /locus_tag="ACG808_001302"
     CDS             complement(303..605)
                     /locus_tag="ACG808_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFS5046936.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(863..976)
                     /locus_tag="ACG808_001303"
                     /pseudo
     CDS             complement(863..976)
                     /locus_tag="ACG808_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1359..2591)
                     /locus_tag="ACG808_001304"
     CDS             complement(1359..2591)
                     /locus_tag="ACG808_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFS5046937.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2845..3414
                     /locus_tag="ACG808_001305"
     CDS             2845..3414
                     /locus_tag="ACG808_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5046938.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(3592..4032)
                     /locus_tag="ACG808_001306"
     CDS             complement(3592..4032)
                     /locus_tag="ACG808_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFS5046939.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            4190..4954
                     /gene="map"
                     /locus_tag="ACG808_001307"
     CDS             4190..4954
                     /gene="map"
                     /locus_tag="ACG808_001307"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFS5046940.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4986..5909
                     /locus_tag="ACG808_001308"
     CDS             4986..5909
                     /locus_tag="ACG808_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFS5046941.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5985..7124
                     /locus_tag="ACG808_001309"
     CDS             5985..7124
                     /locus_tag="ACG808_001309"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFS5046942.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            7117..7917
                     /locus_tag="ACG808_001310"
     CDS             7117..7917
                     /locus_tag="ACG808_001310"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS5046943.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            7917..8744
                     /locus_tag="ACG808_001311"
     CDS             7917..8744
                     /locus_tag="ACG808_001311"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS5046944.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            8749..9456
                     /locus_tag="ACG808_001312"
     CDS             8749..9456
                     /locus_tag="ACG808_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS5046945.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            9556..10422
                     /gene="rfbA"
                     /locus_tag="ACG808_001313"
     CDS             9556..10422
                     /gene="rfbA"
                     /locus_tag="ACG808_001313"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFS5046946.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            10436..11008
                     /gene="rfbC"
                     /locus_tag="ACG808_001314"
     CDS             10436..11008
                     /gene="rfbC"
                     /locus_tag="ACG808_001314"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFS5046947.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            11030..12058
                     /gene="rfbB"
                     /locus_tag="ACG808_001315"
     CDS             11030..12058
                     /gene="rfbB"
                     /locus_tag="ACG808_001315"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFS5046948.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            12156..13007
                     /gene="rfbD"
                     /locus_tag="ACG808_001316"
     CDS             12156..13007
                     /gene="rfbD"
                     /locus_tag="ACG808_001316"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFS5046949.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            13042..15075
                     /locus_tag="ACG808_001317"
     CDS             13042..15075
                     /locus_tag="ACG808_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046950.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            15119..16399
                     /locus_tag="ACG808_001318"
     CDS             15119..16399
                     /locus_tag="ACG808_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFS5046951.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYRWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            16609..17415
                     /locus_tag="ACG808_001319"
     CDS             16609..17415
                     /locus_tag="ACG808_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5046952.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            17427..18647
                     /locus_tag="ACG808_001320"
     CDS             17427..18647
                     /locus_tag="ACG808_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5046953.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            18637..20223
                     /locus_tag="ACG808_001321"
     CDS             18637..20223
                     /locus_tag="ACG808_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5046954.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            20262..22400
                     /locus_tag="ACG808_001322"
     CDS             20262..22400
                     /locus_tag="ACG808_001322"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS5046955.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     ILEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
BASE COUNT         7112 a         3788 c         4646 g         6910 t
ORIGIN      
        1 tacagtgctt ttaaaaaatc tgatgagtat ttgaaagaaa attatgatat tatttataat
       61 ggaagtagcg gaaaaggaag ccaccaaacc gtttggaatt tgttttcaac agttaaggaa
      121 ttgaatacct taggaataca taatagtggg tatagaatgt tagagaaaag aaaatgtgct
      181 gactatattc ctaatgaaac cattacgaaa acagatatgg cgctgatgaa tcgagaagca
      241 gaaaaaataa taaacaaact cacctaatga atttcaagcg agtttgtttg ttattttttt
      301 atttaatctt tgtatctttc tggatcaacg aaagtatact ttatatagtc ataacgccga
      361 tgatcgctac gtgcgtccgg cacgtcagtc acgatatcaa acaaaaagta tacacccttc
      421 ttcattctag ttttcgcagc aggaattttg aaatagttct tattagaata gtagagattg
      481 attaataagc tatcttcgat tgctaaaaag aaaacttctg aattccatac tttataaaaa
      541 tctttgataa atctattcga agggtcaaat ttaaaccata attgtgtttt tccttccatc
      601 agcataaaag ttcacctcaa aaagagtata cgaacgaatg ttctttttgt gaaggcggaa
      661 tttaatatac tgaataatag aaaacaagta cttgtgccaa attatgtgcc aaaaaaattc
      721 gaattcaaca aaatgtaaac aaatagaaaa ccttctatat taagatttcc tgcaataaca
      781 aacacaacag aagacgtgta atagttagtc aggaacgtac aaataacccc tgtatccttt
      841 gcggtacagg gggttatttt gtataagtag acattgtagt ttctaaactg ctatgtccta
      901 aacgtttcga tacgctaagt atatttactc cttgataaag taaaatagat gcatgcgtat
      961 gtcttagtcc atcaataaac agtcgaagag aaaataggga aaaattgatt actaaagaaa
     1021 aacttactag gtcgacggtg tagttgattt tcatacttca atagattcga attaaacgat
     1081 ttcgattggc ttataccagt cctatccaac gaaagatata tcgttcttaa ctctagcggt
     1141 ataacttgaa gctttacgta aagccaatgg gaatgaactg gagatgactt tttattattt
     1201 ttagtaatag ctgatgggtt aatacttaca actttccttt tcaaagtacc atataaaaaa
     1261 acggtggggt tgtttagcga aataaaggat ctaagccaat agcttagatc ctttattcta
     1321 tgctttatat tattttttgc atgtgtgcga aagtatactc aagcccaatc gccatttcgg
     1381 aaaatcggta ctgtagagcc atcttcacgg attccgtcga tatccatctt gtcagatcct
     1441 accatgaaat ctacgtgggt ttgacttcta ttcaatccag cttctgctaa ttcttcatcg
     1501 gacatttctg taccgccttt cacactgaag gcataagcag aacctagtgc taagtgattc
     1561 gatgcgtttt catcaaataa tgtattgaaa aagatgatgc cagattgaga aataggtgaa
     1621 gggtcaggta caagtgcgac ttctcctaaa cgacgcgcac cttcatctgt atcaagcagt
     1681 tttgctaaga catcttctcc ttgttcggca gaaaaatcaa caacttttcc atctttaaaa
     1741 gtaaatttca tacccgagat gatggtgcca gcataactca gtggctttgt acttgaaatg
     1801 taaccatcta ctcgatgact gtcaggtgct gtgaacactt cttccgtcgg catatttgcc
     1861 atgaattttt ctccacgagc attatagctt ccagcgcctt cccaaagatg gtttttaggc
     1921 aatccgataa tgatatctgt accgggagca gtatagtgaa gggcagaaaa ttgttctcga
     1981 ttcaattctt ctgctttttt tgctaatttt tcatcatgct tcttccatgc taagacaggg
     2041 tcttcttcat agacacgagt cgttttgaaa atctgatccc ataatgcctc aacttgtttt
     2101 tcttctggca gttctgggaa gacttttgct gcccattgtt taccagccgc agccacgaca
     2161 gtccaactga ctttatttgc ttgagttgcc ttgcgtaaat tcattagtgc tttgccattc
     2221 gctgattgat aagacgctac tcgatggcta tctacaccag caaatgcatc gggatcggca
     2281 gaaacaacgc tgatcctgct tgctcctttt tcaagccatt catccgcttg atcgatttta
     2341 gattgtggta cattctcaat acgatccgtt gcagcatgta aaagaaattc tctttggatc
     2401 tggtcgtcag tccattgaac gataacctca gcggctccta gtttataagc ttcttgtgtg
     2461 atcaatcgag caaggggtgc ctgttccacg ctgatctgta aaacaactgt gtgtcctttt
     2521 tctgtggcta cgccagtttc tgcaattaag cgtgcatatt tttttagaag ttcattaaaa
     2581 tcagataaca taataaaaat tcctcctcac cattttttct ggtaactatt agcatgatac
     2641 catttaatta tgattcaaac aatgaggctt taaataaaaa aaggaggtta tttttttaag
     2701 gttaagtttc atgatatatg ttactacttg ctatttcagc ttgtgaagaa aatcaaaaac
     2761 taattttgca tatcaaaaaa acatgttaca atgaaattaa tcaatggtgg aagcgttata
     2821 aaaaaattaa ggagattaaa aaatatggca agaaaaaaga cgattactag agaacagatt
     2881 ttggcagctg cttatgaagt agctgccacc gatggattta cacgatttac tgcgagaaac
     2941 atcgcgaaca aaatgaattg ttccacgcaa ccgatttatc ttgagtttaa aaatatggat
     3001 gacttgagac aagctctttt tgagcagatc tatgatcatt tacaatatga agtgttcact
     3061 gttgagcata cagggaatac gattatcgat cttgctttta attatatcca ttttgcaaga
     3121 agagagaaga agttgtatcg ttcactgtac ttagaagaat atggtggagg aaaagagatg
     3181 cagaatttct cttatactta ctttgtgaat acagtgaaac gcgatccagc ttatgtgaat
     3241 ctttcagaag agcagatcga atctttgtat aatgggacat ggatcatagc tactgggatc
     3301 gcagctttga tgtcttcaag catcatccat ccaactgatg aacagattga aaagatgatc
     3361 caagacagta tagatgctat cttgaaattc aaagaaccaa tcgaaatcga ttgaatatac
     3421 tcaaaagttc cacaaatcag taggtaacaa gagatgtgtc aaaataagga aaagttcgtt
     3481 tttcttacag cgtatgcatt tttaactccc gaagaactct tccatgaatg caatgaataa
     3541 aagagccata cagattgaat aaatctggat ggctctttta ttgcaagtat attaaatttt
     3601 tgcgactagt tctttagcga aagcttctaa attttcgata tcttcttctt cagcattcag
     3661 atccacttta acattttcgg ctccttttga tgcgcctgtt tttgcaaaag cagcatcgaa
     3721 atcatccacc gatttgcaga attcatcgta aaaagtatca ccagatccgc agacaccgta
     3781 aatttttccc aatagatcca attcttgcaa gtcttcataa aagtctacga tctcatcagg
     3841 cagatcgcca tcatcgtatg tataagtagc tactacgcaa atatctgcct cctcgaattc
     3901 atctgcatct acttgggtac attcgttgat ttcgacttcg atatccagat tttcaaatgc
     3961 ttctgcaaca atatcggcga tttcttcagt gtttcctgtc atgctagcat atacgatttt
     4021 tgctaaagcc atgatgtgcc tcctcgttta taattattta acaattatga attataccaa
     4081 aacatcattg aaaagaaaat aaaacctaaa gacacctggg gaatgtcatt tggctaaatg
     4141 tatgccgcta tgttaaaatg agagggattt gtaaaaagga gacgaaaata tgattacttt
     4201 aaaatcagca cgtgaaattg aaatgatggc agaatctgga gctttattag cagatgtaca
     4261 taaaaatctg cgtgatttta taaaaccagg aatcacaagt tgggatattg aggtatttgt
     4321 cagaaactat atcgagagtc atggcggaat tgctgcgcaa attggctttg aagggtatga
     4381 atatgcgact tgtatcagta taaatgatga aatttgtcat ggattccctc gtaaaaagcc
     4441 tttgaaaaac ggtgatttgg taaaagtcga tatgtgtatc gatttgaaag gcggcatttc
     4501 tgattcttgt tgggcctatg tagttggaga atctacacct gagatcgacc gattgatgga
     4561 agtaacgaag aaagctttgt acatcggtat tgagcaagca caagtaggga atcggattgg
     4621 tgacatcggt catgctatcc aaacttatgt agaaggcgaa aatctagcga tcgttcgtga
     4681 ttttattggt catggcgttg gtcctacgat tcatgaagaa ccagcagttc ctcattacgg
     4741 agaagctgga aaagggttgc ggttgaaaga aggcatggtt atcacgatcg aacctatggt
     4801 taataccgga acttggaaaa tgaaaatgga tcctaacggc tggactgcgt atacaagaga
     4861 cggcggactt tcttgccaat atgaacatac attggctatt actaaagatg gtccaagaat
     4921 tttgacatca caaggagaag aaggcactta ttgatctatc cagtcaatag gtacgggaga
     4981 ggtacatgaa agtttggaac aaaataaaac aaaatgaaaa tctgatgcgc tttattgaga
     5041 cgactcaaaa gcgcatcact gattcagaaa tgagtacgac ctctgtcgtc gttgcttatt
     5101 atttactctt gtctttattt ccattgatta ttgcttttgg taatatttta ccgtttctcc
     5161 acatcgatca agagactgta ctgacctatt tacgagaagt gattcctgag accgtttacc
     5221 agtttatcgg tcctgcaatc aaagaccttt tgacacaaag ttcaggaggc ttgttatccg
     5281 tttctgcatt agcggcttta tggtctgcaa gtcaaagtat caatgctttg caaattgcga
     5341 tgaataaagc gtatggcgta gaaaatagaa aaaatttttt gatcgtccgt ttcttctcac
     5401 taattgtcat tgtcttgttc atgattgcta ttagtggggt aactatcgtt cttgggttgg
     5461 gacagctgat tttggatgct attcagccaa tcgtacaaat acccgtgaac tttatcgatc
     5521 agttccaaac tttgaagtgg ccaatcacat tggtagcttt atttgtcatc atgttcttga
     5581 tttatttaat tgtccctaat gctcagttga agttgaagac gatcattccg ggcacgatct
     5641 tcgctactgt cggctggatg ctgttatcac aagtgtttag tatctatgct aggtattttg
     5701 caacgaggat cagcggttat cagatcatag gaagttttat tgtactcatg ctttggctta
     5761 attttgctgc tacgatcatc attttgggcg gaatcatcaa tgcagttgtt caagaatacg
     5821 tgacgggtca tcaaatcaaa aaacgtcgag atccaagccg aaaatggttc aataaattga
     5881 aaactaaatt ttctcataaa aaaaagtaga aacaagagtt cttattgtaa atttatatca
     5941 gtaatttata ataattatga ctcttaatta aagtaggtgc ccatatgctt gtttctttta
     6001 gtatggttat aagatttttt ctgacaattt tattatcact gatattaaca cctgtattca
     6061 aaatcatttc agtacaaaca ggtatggtcg acaaaccaaa tgaaagaaga ataaataaag
     6121 ttccaatgcc ttcagcgggc ggactgccta tctttatttc atttgttata tcgactttat
     6181 tcttattccg taaaattatc cctcaatcgt atattgtacc gatcttgatt gcttctttag
     6241 taatcatagt cacaggagtg ctcgatgata aatatgagct gacacctaaa caaaaaagta
     6301 tagggatctt gattgcttct cttattattt attttgtagc agatatacgt attgattcct
     6361 tcacgcttcc ttttattgga tatatccaac taggctggct tagttttccc gtaacgattt
     6421 tttggatctt cggtatcacg aatgcggtca atctgattga cggtttggat gggctggcgg
     6481 cagggatttc attgatcggt ttaattacga taggaatcat tggatatttt ttcctccatg
     6541 cttctacagt gtatattcca gttgttattt tctgtcttgt agcaagtatt atcggttttt
     6601 ttccttacaa tttttatccg gcaaagattt atttaggcga tacaggagct ctattcctag
     6661 ggtttatgat ggccgtcctt tcactacaag ggctgaagaa cgtgactttt gtttcatcga
     6721 tttctttgct gatcgtcatg ggagtgccgg taacagacac gttttttgct atcattagga
     6781 gaaaagcaaa ccgtgtttct ttttctacag cagataaaaa acatcttcat caccgactaa
     6841 tcagtttagg ctttactcat aaaggtgctg tcctgacgat ttatgcgatg gctttgatgt
     6901 tttctttcac agctatggtc atgaattata ctggaagaat cggaacgatc atattgatta
     6961 ttgccatgtt gtttgctgca attttattat ttgaattgat tggtctgatc aatgagaaac
     7021 atcagtttat tttaaaaaca ttgcgatttt tagggaataa agagtaccgt acgcaagtaa
     7081 aacagaaata tttgaagaaa tggaaccgtc atatagatga ataacaaatt aaaccagccg
     7141 atccttatta gtattgtgac ggcaaacagt gataaaattt ttcggacact agataatttt
     7201 atttcaactg taaaagatga aacagcagta aaaatccgaa tttttgacaa caattcactt
     7261 cccgagtaca aaaaaagact aacagagtat aggaaatatc cctacatcga tatctatttt
     7321 catgatgaaa attccggatt tggttatgga cataatcaca atctgatagc tagcgattct
     7381 cgctatggca ttatttgtaa cccagatatt ttagtggatg aagagacagt tacagcattg
     7441 gtcttgttat tgaaagaaca tccggaatgt gcgatgttag cacctaagat tcttaatgaa
     7501 gatggaacaa ctcagcacct catccgtgag cagctggctg tttttgatta cgttttgcgc
     7561 tttgtgcctt tcaaatgggt caaaaaaaag tttgacaaac gactggctgt gtttgaatgc
     7621 cgaggattgc ctactgatgc taatagttat gtacggatga tctcaggtag ttttatggta
     7681 gctgatattg ataaatttaa agagataaag gggtttgatg agcgtttctt catgtacttt
     7741 gaagacaacg acttgtgttt gagttttgaa aaagcaggtt acaagttatt gtacacaccc
     7801 ttttatgcag ttatccattt ttacgggaag ggtgcgcatc gaaatgcaaa actgtttcgc
     7861 gtgtttttga cttctatgat gaaatttttc aataagtggg ggtggcgttt tttctgatga
     7921 aaccagaaat attagtagtc gttgtgcttt atcagcaact attcagtcaa tctccagctt
     7981 atggaccatt aagcaaagca cttagcgaaa agcaaattca gcttattatt tacgataata
     8041 gtcctcaaag gcagcaaaat gagctgtttg aaaaagaaaa tatcgtttat catcatgatc
     8101 cgcaaaatcc tggtttagcg gtggcttata attttgcgtt gtctcaagca gatgaaggaa
     8161 cacgttgtct cgtcactctg gatcaggata cgaggctagt cgacgattat tttgaactat
     8221 tgcgtaaagt aacctttaca gatgaatgtg ttgctgcagt tccgatgatt ttttctggga
     8281 gccgacaagt ttctccccta tattcagatc gttatatcaa tcgtacagct caagcagtag
     8341 aagttgaagg cccaacagct gaacgaatca tggcaatcaa ttcaggtgtt gcctggtctg
     8401 tcaaatttct aaaaaagatt ggcgggttta atcctgcttt ttctttagat tttttggatc
     8461 attggctgtt ttggaaagtg gatcagttgg aaaaaactgt ggagatcctt cctgtacgaa
     8521 tggaacatga tctttcggtt ttagactatg aaaaagtttc aagtaatcga taccgttcta
     8581 ttttacaagc agaaaatcga ttttatcatg aatatgatca aaggcattta tccagtcacc
     8641 gacgacaatt attgttaaga acagctaaac agttcttgac cgtcaaaaac cggcatatct
     8701 ggcggatgac tttgcgctca tatgttaaga attggaaggt gtgaattcgt gcgttcggtt
     8761 tgtatagcta cctttaatgg aggcagatat ttaagagagc agttagatag tattttatct
     8821 cagctttcag cagaagatga agtagtcatt tctgatgatg ggtcagtaga tgatacatgg
     8881 gatatattaa ctgaatacgc agaaaaagat aagcgaatcc agcttttttc ggggcctcgt
     8941 caaggattga tcgctaattt tggttatgcg atccagcaga cgaaaggaga tctaatcttt
     9001 ttggcagacc aagatgatgt ttggttggag acaaaagtga aaaagatcac tgactatttt
     9061 gagcagaatc cggatcagct agtagtagtt tctgatttaa tcattgtgga cagcgagctg
     9121 caagagatcc atgattcgta tttttcgttt cgaaattcca aagaaggatg gcttaataat
     9181 gtgataagaa gtcactatat tggtgctggt atggctttcc ggtcttcttt aaaacccgat
     9241 attttgccga tcccgtcaga agtgccgatg catgatatgt ggattggttt gctgggagga
     9301 aagaagactg ggttcctaag agaaccgtta actctgtacc gaagacatga tttaaacgcg
     9361 agtgagattg aaacacattc taaattacgg caaaaaattt cttggcgtgt tcatctgatt
     9421 acggcgttga tcaaacgaaa atggtttaaa cgttgataaa agatgtttaa atccttaata
     9481 tttccatgac aaatataaca attgatgaga taataacaaa gtgaaatttg aaatcaaaaa
     9541 ggagagcaga tatccatgaa gggaatcatt cttgctgggg gcagcggaac acgtctgtac
     9601 cctttaacaa aggcaacatc gaaacaattg atgccgattt atgacaaacc aatgatttat
     9661 tatccaatgt ctactctgat gttggcggga atcaaagaaa ttctgattat ttcaacgcca
     9721 caagatacac cacgtttcaa ggaattattt ggaaacggaa atgatttagg actgcagatc
     9781 gaatatgcag tacaagaaag tccagatgga ttggcacaag cgtttatcat tggagaagaa
     9841 tttattggag atgatagtgt ctgcttggta ttaggggaca atatttacta tggtggcggt
     9901 ctgtctaaaa tgttgcaacg agcagcttca aaagatacag gcgcgacagt atttggctat
     9961 catgtcaatg atccagaacg ctttggtgta gtagaatttg atgagaatat gcaagctttg
    10021 tcgattgaag aaaagcctgc tcagccaaaa tcaaattacg ctgtaactgg cctttatttt
    10081 tatgacaatg aggtaatttc tatcgctaaa ggtatcaaac cttcagaacg tggagaacta
    10141 gagattacgg atgtcaataa agcatatttg gataaaggaa aactatccgt agaacttatg
    10201 ggacgcggat ttgcttggct tgatacagga acacatgaat cattgcttga agcatctacc
    10261 tttatcgaaa caattgaaaa acgtcagaac ttaaaagtgg cttgtttgga agaaattgct
    10321 taccgtatgg gctacatcga taaggaacaa ttgctggccc ttgcacagcc attgaaaaaa
    10381 aatcaatacg gtcaatattt attgaatttg gccgctgaat aaggagtcct tacagatgaa
    10441 agtaacagat acaaaattaa cagatgtcaa aatcgtagaa atggacgtct ttggcgatca
    10501 tcgcggtttc ttcacagaaa gttattcaaa agcaaaattt gcagaacatg gattgaaata
    10561 tgactttgtt caagacaacc attcactatc agctgaagct ggtgtccttc gggggcttca
    10621 tttccaaaaa ggagaagctg cacagacaaa attgattcgg gtagtcactg gagcagtttt
    10681 agatgtagta gtggatatcc gcaaaggtag cccgacttat ggtcaatggg acggatttat
    10741 cttatctgaa cataatcatc gacagttgct tgtacctaaa ggatatgccc atggattcgt
    10801 tactttgaca ccaaatgtga acttccttta taagtgtgat aattactata atgcagaagc
    10861 agatgggggc atcgcatttg atgatccaga tttagcgatt gattggccaa ttgatccatc
    10921 aagagcgatt atgtcggaaa aagatcaccg tcatccgaca ttaaaagaat ttgaagcaga
    10981 aaatccattt gtttacggag aaatctaaga attaaggaga aattttctta tgaaaaacat
    11041 cattgttacc ggaggtgccg gattcatcgg ctctaatttc gttcattacg tggtaaacaa
    11101 tcatccagaa gtgcatgtca ctgttttgga taaactgact tatgcaggaa acaaagagaa
    11161 cttggccgga cttcctagcg atcgcgtaga attggtcgta ggagatgttg cagatgcaga
    11221 attagtggac cgtctagtaa aagaagcgga cgcagttgtt cattacgcgg cagaatccca
    11281 taacgataat tcattaaaag atcctttccc atttgtacaa acgaatatca ttggtacgta
    11341 tacactttta gaagcttgcc gcaagtatga tgtacgctat catcacgtat ctactgacga
    11401 ggtttacggc gatcttccat tacgtgaaga cctgccagga catggggaag gcgaaggaga
    11461 aaaattcact gcggaaacac catataatcc ttcaagtcca tattcttcta caaaagctgg
    11521 ttcagatctg ttagtaaaag catgggtgcg ttcttttggc ttacgtgcaa ccatttcaaa
    11581 ctgttcaaac aactatggtc cttatcagca tattgaaaaa tttattcctc gccaagtaac
    11641 gagtatctta agcggtattc gtccaaaatt atatggcgaa ggaaagaatg ttcgtgactg
    11701 gatccatacg aatgatcatt cttcagcagt gtggctgatt ttgacgaaag gcagaattgg
    11761 cgaaacctat ctgatcggtg cagacggtga agaagataat aagaccgtga tggaattgat
    11821 cttagaaatg atgggccaac caaaagacgc atacgaccat gtaaacgacc gcgcagggca
    11881 cgatttgcgt tatgcaatcg attcgacaaa attgcgagaa gagctaggtt ggcagcctga
    11941 gtttacgaac ttccgtgatg ggttggctga cacgatcaaa tggtacgaag aacatgaaga
    12001 ctggtggaaa aaagaaaaag aggccgttga agcagcatat gccaaaaatg gacaataata
    12061 cagaggaaat ttcactgtat taatggaaaa acacatgtga ggcaggcgct cgcctgtctc
    12121 ttttttaaaa taaaaatcaa ggagcggata accatatgtt tttattaact ggaggaaatg
    12181 gacaattagg tacagaattg cgccatttat tagatgaaaa aaatctgcca tatgtttcaa
    12241 cagatgcaaa agacttggat atcacagatg ctgagaaaac aatggcttat attacagaat
    12301 taaaacccga tgttattttc cattgcgcag cttacacagc agtagataaa gcagaagaag
    12361 aagcaaaaga attagatgag aaaatcaatg tcgatggcac aagaaatgta gcacttgcag
    12421 caaaagctgt gaatgcacaa ttcgtctata tcagtacaga ctatgttttc gatggaaaaa
    12481 acaaaaatgt agaatacgaa gtagacgacc aaacgaatcc actaaatgaa tatggtcgta
    12541 cgaaattatt aggcgaaaaa gcagtagaag agattttaga agactattat atcatacgaa
    12601 cttcttgggt tttcgggatc tatggacaca atttcgtctt cacgatgcaa cgcctagcgg
    12661 aaacccgcga caaactgact gtagtaaatg accagtttgg gcgaccaact tggacacgta
    12721 ctttggcaga atttatggta tatgtcatcg aagaaaaagc accatttggt atttatcatt
    12781 tgtcgaacga aaatagctgt tcttggtatg agttcgcaaa agaaatattg aaagatacag
    12841 atgtagaagt tgcacctgtt acgtcagaag aatttccgca aaaagcaaca cgtccgcagt
    12901 attcagtcat gagcctgaaa aagacagaag cgttaggttt tgtcatacca acttggcaag
    12961 aagcacttgc gcaaatgctg gaagctgcca aaaaaatgaa taagtaattt cctttttatc
    13021 aaatagaatt tggagcatga tatggaaaaa caaacaagaa gtgcacgacg aaaagaaaag
    13081 ccaaaagcga atcaggcaga ttcgaatttg gtgcttgacc tgtttctaaa actccgctat
    13141 cttattggcg ttttagtcat cgtggtcatc gttactttta atttaaacgg aagttcactt
    13201 ggtgtttggg ataaatatgt gtcgcaaaga gacgatggga aaaaaactga tgtgattttt
    13261 ggacagaatc gggaagtgcg gtcagacgaa tggctggtgc agacaccttt ttacctctca
    13321 caagctgaag aggacttttc gttggtgaac gaggattatt cattaaacgg acaaaacatg
    13381 atcattgcgt ataattctcc tgtaaaggat attacggtta tcggaaaacc attcaactgg
    13441 ggattctttt tcctcggaag agatcgagga ctttcctttt attgggcgat gaaattagtc
    13501 gtcatggtct tgcttggatt cgaagtactg atgatattaa cgaagaggaa aaaagctcta
    13561 tcgctaatcg gtgcatttgc gctgacattt tctccagcag tccagtggtg gttcatgcaa
    13621 catgtgggcg atttgatttt ctttactttg ggactgatga ttgcttttta tcattatttc
    13681 tacaatcatg ataaaaaatg gctacgaacg ttgatgatgc ttttggtggt tattttcggt
    13741 attgggttta ttttagtgat ttacccagcc catcaagtga tgctggcgta cttgctgatt
    13801 ttttattttg ttggcctgct tatttactat agcaagaaag tttcatggga tctgttggat
    13861 gtagtgttga ttggaggggc agttttattt attggcggag tcatggttca tttctggcta
    13921 acgtcaaaag acgcattgtt agcttctttg aataccttat atccaggaaa acgggtaagt
    13981 actggaggaa agtggacgat tggcgaattt ttttctttcc ttacaaattg gaaaattcct
    14041 tttgaagata tcaactattc gaataactct gaagtggccc tgttctatca cttttttcca
    14101 actgtattcc tagcaagtcc atttgtgtta tttggcaaaa aagatagcga acaaaagtta
    14161 ctgggacgta ttttgatgat tttttgtctg ttttctatct tttggattac cgttgggttg
    14221 ccaaaatggc tagcagaaat cactctgctg tcttatgttc ctccattaag agccatcctt
    14281 acttttagtt ttgcggcttg tttattatcg atctggttta tcgcttatat ttggagagaa
    14341 gatgttattc ctatttggta taaattcatt ttgttggcag taaatgcatg gtcttatttc
    14401 tatgcgatta cgcattcgaa aatggataat tatttttcgg attttgaaac catcgcagtt
    14461 gctgtgttgg gaacagtgat tttagctttt gcattatttg gctggagacg cttgttttac
    14521 ctcgcttttg tcgccttagt tatcgtatct ggctttttcg tcaatccagt cgtagaaggt
    14581 acaggagcga tttttgaaaa aacactggca aaagaaatcc aaaaaatcga tcaacaagat
    14641 cctggtaaag tttggttgtc tgaagatgaa ttatataact tcaccccaac attgggagtg
    14701 aaatccttca atacagtacg cttttatcca gatatagctg catggaagaa aatcgatccg
    14761 aaagaagaat acgagaagta ttataatcgg tatgctcata caagagcttt tatcgctaag
    14821 gaagatacaa agttcatttt agatcgtcca gatatgtttt cggtcacgtt gaattttgaa
    14881 gacgcagaaa aattagggat cgactatgtg atcagtaaac gaccattaac tgattacaac
    14941 caattatatg aagcgcagtt cacgcagcta tatggaccgg ataaagacgg ttatatgatt
    15001 tttaaagtag aatatccgga ttatccagat gtaccacaaa cacaaactca ggaaaatcag
    15061 ctagtcgtac agtaataggc tagctgagaa taatcctgaa atattagtag aaaggagagt
    15121 gaagataatt caagcaatcg tacaaaaatt tgtcaacaaa atgagtgtcg gtttcttgct
    15181 tttgttgctg acgctagtct tttttgtttg gacagcaggg gtaggttttc cagcaacttt
    15241 gtttatatat aacaatagtt cattacttat cgcttttagt gtggctattt tgctcatttt
    15301 aaatgtttat aaagcaaaag gcgtcgattt tctttatcta ggtgttgcgc ttgccctttt
    15361 cattttcttt tcttactttc gggaattaag aaatgagtct ttatttggag acttactgtt
    15421 tcctttgatt attttatttt gtttagtaat taaatggatc gagtggacaa aatgggatcg
    15481 cggtatttat ctgattgtct ttacggtctt cctcggaatc acgttatatc gtgtatttac
    15541 agagatccaa gtaccagaag gacaaagcat ctgggctcca ggcaattcgc tttctgatat
    15601 ttggatcaat acgaatacaa tcggaagttc tttgatgact cttgctttat tgatcagtgg
    15661 atttgccagt tcttttgaac gatggtatgt tcgaatcatc ggtgggatag cagtcgttgc
    15721 agccttttta ggtatttggg tctgccaatc tagaggtgca ttagtcgcgt tgattgtttt
    15781 tgcgatattg gatcttttac ctaaatcgtt gatgcgtgtc attcgtgcgc catttattgc
    15841 ctacacggtt acgattcttt tagctttgcc gatttcttat ctggcggctg tctctgaaaa
    15901 agtcaatttg tttaccggtc gagaagatat ttggcataaa ttctatcaga cgctaggaga
    15961 aaaatctgaa caagttttac taggaatgaa aacatttatc ttccagcgag gaaaccaatt
    16021 tttaggaaac cacaatagtt ataattctat tttgaatatc tatggattga taggatttgg
    16081 gattgctgct ttgctgctta tcctttttat tggtcgctta acgttgaaag cagatttatc
    16141 caacgggcag atgactttta tctgggcctt ttttgcggtg atgatgcagt catttatgga
    16201 ggatacattg acttcatacc gatgggttcc gattatttat atcctgttgg ctatggccgt
    16261 tcatcgatat gatgaaccag aagttacgga ggaattctat gttatggaag aggcatatga
    16321 cgatatgact tatgatgata catcttatga ggaacaacca gatagcgctc attcttccag
    16381 gatccaacgc tatcattgat aggcacaaaa tacgagagag gctgtgacaa ccgtcgcagc
    16441 cttttttggt ttattgcaat tgtctagcat cttctgtaaa atagaaacgt tagagaaaat
    16501 cttgtatcat agaaaattaa gaaagaaata agaacgcagc tcggtaccaa aaatatggta
    16561 aaatggcact tgactgtaat tcactgaaaa tcgcttggag gaacacaaat gtttaaagat
    16621 ttgtatttat ttatgaagga catttatcag aacagaaaat tactgctaca gttttcgatc
    16681 aatgacttca aatcaagata tgcaggatcg tttttaggaa tcttatgggc gtttatcaat
    16741 ccgctattta ctgtattgat ttattggctg gtatttggtg ttggactgaa agcacaaatg
    16801 atcgatgcaa aatacccatt tatcgtatat ttagttaccg ggatgattcc ctggttcttc
    16861 ttctcagatg catttgcatc aactacggtt gtttttcggg aatatactta tttagtaaag
    16921 aaagtcgtat tcaatattcg tatcttgccc acgacgaaaa tcctatccaa tctgtacacc
    16981 catttattct tcattctgat tggcatagct gtcgcgctta taaatggtat atatccttca
    17041 gcaatgtctt tacagctgat ttattattta ttttgcatgt gtgctttctt gacaggattg
    17101 acttggctga cggcatcgat ccaaccattt ttacctgata tcatgcaatt aattacgatc
    17161 gtgctgcagt tgatcatgtg gacattaccg atattatgga gccctaatca gtttgattca
    17221 ggtcttgtaa gcatattgaa attaaatccg ctgtactatg ttgttcaagg ttatcgtgag
    17281 tcatttttaa gtgaagcttg gttctgggag catggaacat atactttgta tttctgggtt
    17341 ttcacgttag taatgttcgt aatcggctct gcagtgttcc gtagattgaa accacatttt
    17401 tcagatgtgc tgtaaggaga atagctatga cagaatatgc aattagacta aaagatatca
    17461 caaaatctta taatatgtac gcaaaacctt cggatcgatt tcgtgaagcc ctcaatcctt
    17521 ttaagaaatc ctatcatgat gtattttatg cgttgaaaaa cgtgaatgta gaaataaaaa
    17581 aaggtgagat gatcggtttc gtgggagaaa acggatcggg aaaatctaca atgttgaaaa
    17641 tcattacggg agtactgaca ccaacatccg gcacgatgga aatcgaagga aaaatctcag
    17701 ctttgctaga actaggatca ggattcaatc ctgagtattc tggctatgaa aatatctatt
    17761 taaatggaat ggttctagga ttttcaagag aagaagtcga tgaaatggtc gatgatatca
    17821 ttgaatttgc tgacatcggc gaccatattt atcaacctgt aaaaacctat tcaagtggga
    17881 tgttcgttcg tctagctttt gctgtagcga tcaatgtgaa tcctgatatt ttgattgtgg
    17941 atgaagcatt ggcagtagga gatcttgaat ttcaactaaa atgtatggaa aaatttacag
    18001 aaatcaagaa ctcgggaaag acgattttat tcgtttctca tgatattagt tcgattcgcc
    18061 gtttttgtga tcgttcctat tggttgaaaa atggaaaagt tgtcgaatat ggtgatacga
    18121 tggaagtaac gacgaattac gaaaactttt tgaagaaaaa atcagttaaa acagtagatc
    18181 gaaataaaaa ccaagtagaa cgcttcgcag ctccagatat cgtagacgtg acgaaagccg
    18241 aattgttgga taaagataaa aatccagtgg atatgctgga acaaggagaa aaactatacg
    18301 ttaaagtaac ctatgaagta aaagacgata caatcaaaaa tccagtttta ggaattgctc
    18361 tgcgtacagt cgacaatttt tatgtgtgcg gactcaatac tctactagat ggtatcaaga
    18421 tcccttggaa aaaaggtgag aatatctttt atctggagta tgaaaagtta ggtttgttag
    18481 ctggtgaata ttattttgac gttgcggtct ttgaagaaaa tgcaacggtt cctttggtat
    18541 acaagacaaa atacatgaac ttgtttgtca gcggttctta tatcggagaa ggcatcgtgg
    18601 tcttagacca taaatgggaa gaagggactc atagcaatga aatatgattt tgaaatggac
    18661 ctagatgaac aaagcagtgt gggaaaaatt gccgcacaga tcaaaccagg aagtaaagtt
    18721 ttagagtttg gtcctggaaa cggccgatta acgaaacatt tgatcggagc aaaacaatgt
    18781 gaagtgagca ttgtcgagct agataaggaa ttgttcgatt ttgtcagcga gtttgcccaa
    18841 gatggatttt atggagatat cgaaagtttt gaatgggcaa actattatgc tggacaaaca
    18901 tttgattacg ttttgtttgc cgatgtttta gaacatttgg tagatcctgg aaaaacgttg
    18961 aagaaagtta gagaattttt gaatgaagaa ggagaaattc taattacatt ccctaacctt
    19021 gcgcataatt ctgtcatgat tgatttgttc aataatcaat tgccatgggc ttcttatggt
    19081 ttgctagatg aaacccataa ttctttttat actcatgatg gcttccaaaa agtttttgaa
    19141 aaagcaggat tatacatcaa tattgaagat tatttgtact tagctgtagg tgatacagaa
    19201 ttaaaatcaa cttatgaaga attgccggaa gctgtacgtt atgatttcaa aatgcgacca
    19261 tttggcgaag tctaccaata cttcttctca ttgatgaaac atccagtagc acaaagttcc
    19321 attgcgcagc cgcaaaattc taattatgtg aaagtattag aagtaatcca gcaaacgaaa
    19381 caagatgaaa taattcaaca aataccattc aataatttca caggggaaaa tgagacacta
    19441 agtttccatg tatcagaatt agcagaacga atggtgtttc gcttcgcgca gcaacctagc
    19501 tttatcgaat tcagcgcaga agctgctgga gaaaaactca catttatcga ttcaaatgct
    19561 gtagtcaaaa cagtaaatga ttgttatttg tttgatggta aagatttacc agagttcttt
    19621 ttaacggata ttgcagggaa agaagtgact atccattgtc actatcgttt tatcggagaa
    19681 ttgacaccaa caatgaaaga attgctggaa acaatccgtc caatggctga agttacaaag
    19741 cagctttctg agaaaaatga tgaattagaa agagaaaatc attatcttat tgagaataat
    19801 acaagattag atcagacgtt gaaaacaacc ataaatcgct attgcacgtt gttggaaagt
    19861 gatgaatgga caatcaaaca tagattaggg cgacgtaaga aagaaacatc gaaaaaaatc
    19921 caagaaaaag agctttctct atgtattgac gaaaaaatct gggatgcaga aacaaagata
    19981 ttgaaaatca tcgggtgggg aatcgctaat tcggatcgcc aaccattgtc ctataagtta
    20041 tcagccgacc aatcaccatt ttttgaagca attcctgttt tacgagaaga agtcaatcaa
    20101 gcaaaacagc tagctgaagg agcaaaagca ggatttgaac tgcggatcct ttgcgagaag
    20161 gaacgttcat tcttagttga agcagtggct caaaatggtc aatcatggat cattgaaatg
    20221 taaataatga ttaacttata aagataagga gtaaacgaat cgtgcaaaaa aaagaaattg
    20281 ccgtatttat cgatcaaatc actcgtgaac gggccactgg cgatttgctt attgtgggct
    20341 gggcaattga tgaagtgacc aaagaaatcc ctacgataaa ggtagaaaaa gaaaatgtga
    20401 ttgctgaagc aacacatgtc gtaagattgg atatcaatca tttatataat cttgatgtta
    20461 agacccaaag cggttttaag gtacgtttat cagggaaaat gagaggaaaa gcaatcttag
    20521 acttccaaac agcaagacat caaaatggca tagctgtgaa attaaacggt aaatatcctt
    20581 atgatgacgg cattgaatct tcatgggaaa gaaaaaaaag attactgaaa aaagggataa
    20641 attatgcaag gactcatggc gtaaaaaaag caatccgccg ggtcaaactg gaattgaaac
    20701 caggatcgat cgactatgca gagtggatca gtcgtcatga gaaacctgat ctgaaagagc
    20761 aaaggaaaga agtacaagga tttgcctacc gcccattgat ttccatactt atgcctgtct
    20821 ataacgttga aatcaaatgg ctcgaaaaat gcattgattc ggtacttgat caaacgtatg
    20881 atcactggga actatgtatc agtgatgatg cttctactga tccagctatc cgaaagtgtc
    20941 tagaatccta tcaagcaaaa gatgatagaa ttaaagtcgt tttccgacaa gaaaatggac
    21001 acatcagtct ggcaactaat tctgcattgg aaatggcaga aggagagttt atagcgctct
    21061 tagataatga tgacgaatta cctccatttg ccttatatga agtggcaaaa gtgctaaacg
    21121 tacatccaga actggatttg atctacagtg acgaagataa aatcgatgca gatgggaatc
    21181 gctttgaccc gcacttcaaa gcagactggt ctcccgatac attgatggga aataattata
    21241 tctctcactt aggcgtatat cggacaagca ttgttaagga acttggcggt ttccgtaaag
    21301 gatatgaagg ctctcaagat tatgatttgg ttcttcgtgt gacagaacaa attcctgcag
    21361 aacacattta ccacattgac cgtgtcttgt atcattggcg gacgatcccg ggatcaactg
    21421 caagcaatgg agaagctaaa agctacatct atgattctgg agtgaaagca ttgacagatg
    21481 ctctatctag aagaaatatc aagggaagcg tacatcctgg cagaatttct ggtttttatg
    21541 aaatcactta tgatgttttg caagaagact tggtcagtgt gatcattcca actaagaatg
    21601 gctatgaaga tctaaaaact tgtgtggatt cgatcatcga aaaaacgtct tatccaaatt
    21661 atgaaatcat tattgcagat aatggaagta cagatcctaa aatgcaagaa ttatttgcag
    21721 aatacaagca tcagctgaaa gaccgattta tcgtcgaatt aattgacatt ccattcaatt
    21781 attcacgtat caacaatctg gcagcagaaa aagcaaacgg caaatacttt ttattcttga
    21841 ataacgatac agaagtgatt gaacctgact ggatgacagc gatggtttct tatgcacagt
    21901 ttgatcgaat cggctgtgta ggagcaaaat tattttatcc ggatgataca acgcagcatg
    21961 caggtgtatt attagggatc ggtggcgttg caggacatgc attgaataac tatgatcgaa
    22021 cacattgtgg ttactttggc cgattggtca ttgacgtcaa ttacttagca gtcactgcag
    22081 cttgtatgat ggtcaaggca gctgacttca atgctgtcaa tggatttgat gaaatattag
    22141 aagtagcatt caacgatgtt gacttgtgct tgaaagttta tgaactgggt cgttacaatg
    22201 tctatgcaca tcaagcagaa ttgtaccatt tcgaatctaa atcaagaggt tatgaagata
    22261 caccagaaaa acaaagacgt tttgctgggg aaatcaaaaa aatgcaagat aagtggcctt
    22321 cttatattgc tcatgaccca ttttataacg ataatctaac taaagaagga atcggcgatt
    22381 tttcgttgag acctgattga ttaaaagaac tgtattttca tttttattaa tgaaaataca
    22441 gttcttttaa tttaat
//
LOCUS       DAZUMK010000036        22105 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000036 DAZUMK010000000
VERSION     DAZUMK010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.036"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            211..1917
                     /gene="efbA"
                     /locus_tag="ACG808_001323"
     CDS             211..1917
                     /gene="efbA"
                     /locus_tag="ACG808_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFS5046956.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2075..2977)
                     /locus_tag="ACG808_001324"
     CDS             complement(2075..2977)
                     /locus_tag="ACG808_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS5046957.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2974..3666)
                     /locus_tag="ACG808_001325"
     CDS             complement(2974..3666)
                     /locus_tag="ACG808_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFS5046958.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3709..4350)
                     /locus_tag="ACG808_001326"
     CDS             complement(3709..4350)
                     /locus_tag="ACG808_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFS5046959.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4388..4702)
                     /locus_tag="ACG808_001327"
     CDS             complement(4388..4702)
                     /locus_tag="ACG808_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046960.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4690..5319)
                     /gene="pyrE"
                     /locus_tag="ACG808_001328"
     CDS             complement(4690..5319)
                     /gene="pyrE"
                     /locus_tag="ACG808_001328"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFS5046961.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5326..6042)
                     /gene="pyrF"
                     /locus_tag="ACG808_001329"
     CDS             complement(5326..6042)
                     /gene="pyrF"
                     /locus_tag="ACG808_001329"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFS5046962.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(6039..6971)
                     /locus_tag="ACG808_001330"
     CDS             complement(6039..6971)
                     /locus_tag="ACG808_001330"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFS5046963.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6968..7744)
                     /locus_tag="ACG808_001331"
     CDS             complement(6968..7744)
                     /locus_tag="ACG808_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFS5046964.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7798..10983)
                     /gene="carB"
                     /locus_tag="ACG808_001332"
     CDS             complement(7798..10983)
                     /gene="carB"
                     /locus_tag="ACG808_001332"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFS5046965.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10988..12064)
                     /locus_tag="ACG808_001333"
     CDS             complement(10988..12064)
                     /locus_tag="ACG808_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFS5046966.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12069..13349)
                     /locus_tag="ACG808_001334"
     CDS             complement(12069..13349)
                     /locus_tag="ACG808_001334"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFS5046967.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13368..14294)
                     /locus_tag="ACG808_001335"
     CDS             complement(13368..14294)
                     /locus_tag="ACG808_001335"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFS5046968.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14307..15590)
                     /locus_tag="ACG808_001336"
     CDS             complement(14307..15590)
                     /locus_tag="ACG808_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFS5046969.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15604..16143)
                     /gene="pyrR"
                     /locus_tag="ACG808_001337"
     CDS             complement(15604..16143)
                     /gene="pyrR"
                     /locus_tag="ACG808_001337"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFS5046970.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16378..17289)
                     /locus_tag="ACG808_001338"
     CDS             complement(16378..17289)
                     /locus_tag="ACG808_001338"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFS5046971.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17286..17750)
                     /gene="lspA"
                     /locus_tag="ACG808_001339"
     CDS             complement(17286..17750)
                     /gene="lspA"
                     /locus_tag="ACG808_001339"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFS5046972.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17755..18453)
                     /locus_tag="ACG808_001340"
     CDS             complement(17755..18453)
                     /locus_tag="ACG808_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFS5046973.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18440..18826)
                     /locus_tag="ACG808_001341"
     CDS             complement(18440..18826)
                     /locus_tag="ACG808_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046974.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18973..20643)
                     /locus_tag="ACG808_001342"
     CDS             complement(18973..20643)
                     /locus_tag="ACG808_001342"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFS5046975.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20826..21059
                     /locus_tag="ACG808_001343"
     CDS             20826..21059
                     /locus_tag="ACG808_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS5046976.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21158..21589)
                     /locus_tag="ACG808_001344"
     CDS             complement(21158..21589)
                     /locus_tag="ACG808_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFS5046977.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21668..22081
                     /locus_tag="ACG808_001345"
     CDS             21668..22081
                     /locus_tag="ACG808_001345"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFS5046978.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
BASE COUNT         6255 a         4934 c         3902 g         7014 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgataccta
       61 aaaatttgga tttcaacgat agaaacactg ttttttatgt aattagtcca agatttttct
      121 agacttttgc tttactattc ggccaaattg gtttttcttc tttttttcgt tataatgaat
      181 atacttatga aaagaaatga ggaatgaacc atgtcctttg atggtgtatt tacccacgcg
      241 atggtcaacg aattgcgtga aacgttacta tctggccgga tctcgaaaat ccatcagcct
      301 tatgaaaatg aaatcgtgct ggtgattcgt tcacgcggca aaaaccatcg attattatta
      361 tctgcgcacc caagttatgc tcgtgtacag attacccaaa tcgattatca gaatcctgat
      421 aatccgccta acttcgtgat gatgcttcga aaatatttgg atggcgctat tttagaagac
      481 atcgaacaaa tcgaaaatga tcgagtgatc catttccact ttgctaaacg aaacgagttg
      541 ggtgatctac aaaatatcat attgatcgtt gaattaatgg gacgtcatag tacgatcgtc
      601 ttagttaatc gagaaactgg aaaaatactg gatgcgatca aacacattgg aagctctcaa
      661 aacacttatc gttctttgct tcctggtgta gaatatgttg ctccgccaaa acaagaggtc
      721 ttgaacccat ttagctccga gaaagaaaaa atctttcagc ggctttccca aactgaacta
      781 gatccaaaag caatccaacg tcagtttcag ggaatcggtt tcgatatcgc tcaagagctg
      841 accaaaagat tactggaacg tcctaatgaa aagatggttg tatgggacga atttttctca
      901 gccatcagcc atcagcctat tcctactttt tacgaaacgg aaaataaaga ttttttcaca
      961 ccgattgctt atcaggtatt gtccgaacag gcatctgctg taacagctta tccaacactc
     1021 agtcaattat tagatagcta ttatcacgaa aaagcagaga aagatcgggc aaaacaacaa
     1081 ggcggagaac tgattcgaaa aatcgaaaat gaactaaaac gaaataaaaa taaattgaaa
     1141 aaacgtgaac aaacactcaa agaatccgaa aacgctgaga attaccgtag aaatggcgaa
     1201 ctacttacta cttttctgac acaagtacca cgcggcgcaa aagaagttgt tttacctaat
     1261 tattatgaag aagatcggcc aatcaaaata gcactagatc cggcattgac accaaatcaa
     1321 aatgcacaga aatatttcca tcgataccaa aagctgaaga acgctgttaa gctgattggc
     1381 gaacaaatcc aagaagccaa agatgaaatc caatatcttg aatctgtctt atctcaactg
     1441 gaaattgccg gcccaatgga tatcgaagca attaaagaag aattgacttc agaaggatac
     1501 ctgaaaaaga agaacttaaa aaaacaaaaa cgtaagaaac catctcagcc tgatcaatac
     1561 ttttcttctg acggaacgct cattcttgtt ggtaaaaaca atcttcaaaa tgaccagctg
     1621 acaatgaaga cagctaaaaa aaccgactat tggcttcatg caaaaaatat acccggttcg
     1681 cacgtcatca ttaaaagtga caagccctca gatgaaacaa ttacagaagc tgccgagtta
     1741 gctgcctatt tttcaaaata ccgatactct gcccaagtgc cggttgatct tgtacaggta
     1801 aagcatatcc gaaaaccaaa tggcgccaag cctggttatg ttatttatga aaaccaaaag
     1861 acgattatcg tgacaccaga agaagaaaaa attacagcta tgaaacaaaa caggtaatca
     1921 atgagtgttt ctagaatcat atttgcgtac tttcccacaa cagcaattag ttataaattg
     1981 tttgctacaa aaagaggagt tgtgacaaaa ttctgtcaca acccctcttt tttgatcaac
     2041 ggtgttcaat aaacagcgtt ctcatctgct tttcttagct ctgctggtcc agtaaccgag
     2101 taagctcttt gataaatctt tgagcgattt cactgagcgg acgtttcgcc gaataaatga
     2161 tataaaattc atttagttcg ccttctgcca aagggattaa ccgcaagtgc tcgcgaatcc
     2221 ctccaatcac aataccagat cctgaagcgt aagcatcagt ttcaagaacg atattcatca
     2281 aagtacctcg atcactcgta taaatcactg tttcttgatc agggatctcg ataaggtctt
     2341 cataaaaata cgggaaatta tttccatcct gtgtaaaacg aacttgagga taagataaca
     2401 attcttcttt ggtgatctct gcgtgttcag ctaatggatg tccttgcctt aaaaaaatat
     2461 gcgttttgaa ttttccaatg ctgcggccac ttagctctcc ttgttgtaaa tatcttctga
     2521 taccagcttg attatgctca ttcatataaa taatgccgat ctcactgtgc tgctctttga
     2581 cttcttcgat cactctcgct gttgtcgtct ccatgatccg aaattgtttg atctcgtctt
     2641 tgaattcctc gagtagctgt gcggtcaaag gacctaagaa atcataatgc tgggcagcga
     2701 tcgaaaaatt gtctctcttt tcactcgtta gatagtgagt ttctaatgta tcaagctgga
     2761 ccaatatttt ctcggcacga tttaagaagt cttgcccttc ttctgttaaa tatgcccctc
     2821 gattcgttcg agtaaacagc gtgatcccta actcggtttc taattctttg ataccagctg
     2881 aaagacttgg ctgggagaca aacattttct ttgccgcttc tctgaaacta ccattgtttg
     2941 ctacagcgac cacgtatttc atctgctgaa tattcattgc tcaaactcct cgaaataata
     3001 aatttctctt ccattcaagg gctggattgg tcggttgttc ggtcgagcat actgaccatc
     3061 gaaggggaga agaaactctt ggttcagttt tcctacatga tccattacaa accattggat
     3121 tggcccgctg gtaggcggag ttgtcaatga gccaatataa tggaaatggc ttttttgctc
     3181 cggaaagaac acatccggat ttacccaatg ctcatgtttt tctaagttcc aactttcccc
     3241 attttcttga ttatacagcc aaccttcctg taccattagc tggtacatga cacccacaac
     3301 tagattctct tttttgctgt tcatatgaac aaaatgaaat tcaatatctt cctgttttcc
     3361 gttgatgata tgttcagaag gcaagtggaa atgaatatct gtcaaataat agcgttcttt
     3421 cttgaactct acataactga gttggtcaaa aggtactaaa tgaatcgtgt ttttgaattc
     3481 cttgtcagta aaccgttgct tttgataatg aaaggaaagc gtgtctcctt ctattttggt
     3541 ttcttccccc ttggtcaaat gaataggcga ctgcaatgga aattccgcac ctctcgcaaa
     3601 ccaatcacat aaactcgccc aattttccgg acctttgtct ccagaatagc cccattcgac
     3661 atccatattc tttttcaact atacttcccc ctgcatctca tatcattttt atttttcttc
     3721 tttctttaca atcgttgaga cggtttctaa aaacgcctct tttccataaa attcctggtt
     3781 caatagttcc ggaaatatct ctttaaagaa atactgaaca gaaggccgat gttcaaagct
     3841 tttaatcgta gtcactgcct caatgaacat ctccttataa actttttccg ggttattttt
     3901 gatgatttca tcaaaagatt cataaagaag gtcaacctta tctgaaatgg ctaatagttc
     3961 tccttctacg gtttcatctt ttccttcgaa caaccgacgg cgataaactt cttggaactc
     4021 agctggaatc tcttggttga tgaattcgtc cgtcatcttt tcttcaactg tttgcagcat
     4081 ccctcttaac tctttgcttg cgtatttcac cggcgttttg atatcaccaa taaaacgctc
     4141 tgtgtagtca tgattcaatg atttttcata gagcagtttc caatcgatcg gttgtccatt
     4201 cgcttcttca atgtctccta gaacttgcgc aatagatgct acacgatacg aatgtgctgc
     4261 aactgtatgc tctgtatatt taaaaaatcc aggtgccctt gtgatcgtct ctaaattgtt
     4321 caatcctaaa ataaattcat tcaaccccat aaaagttacc tccctttgag atttcttttt
     4381 ccgaatatca tatcattaaa taactgatat tcgcaacatt tccgctcttt accatttttg
     4441 aaacagctcc cttaccaaaa aatagagaaa atctatatat aatagaaaaa agagaagaga
     4501 ttattctctt ctttctgcta accagtctta gaaaaatgaa ttttaaaaaa atcaaaaact
     4561 gatcacgata gaagtttctt ttcatacctg atgaaaagat gcttctcgca atcagttttt
     4621 ggatttttgc tattccaatt gatgattctg gagacacaaa tcggatttct ctatcttcat
     4681 caagcgcgct taaatctgcc aattttcagg atcttttttc cattctttta gcagagttaa
     4741 ttcttgctca tcgatataac gatcttctaa agctgcttca atcaatacag aataattggt
     4801 caacgtcata agagggattt cggctttttc gaaatttgct tttccttttg gcaactcata
     4861 cgtaaagata gccgcaacac caaggatatc cgcaccttct ctttttgctg cttcagctgc
     4921 ttccaagaca cttccgcctg tagaaatcaa atcctcgatc acaaccatct tctgaccttc
     4981 aaatatacgt ccttcgattt ggttaccttt cccatgatct ttcgccttac ttcgaatata
     5041 gaccattggc aaatccagta tttccgctac ccatgcggca tgagggattc ctgctgttgc
     5101 agttccagca atcacttgta cttcagggta agcttctttg attttgcttg ctaatccttt
     5161 tgcgatctct tttcggactt ttggatagct catcgtgatt cggttatcac agtaaattgg
     5221 gcttttgatt ccacttgccc atgtaaaagg attacttgga tttaaaaata ctgcttcaat
     5281 ttctaataag tcttttgcga tactttgttc aacattcatt ttaggttagc tccattctgt
     5341 ttgaatcgtt tgatatgctt tataaggatt agttgactga gtgattggtc gacctataac
     5401 gataaaagtt gagccgatct tttttgcctg agaaggtgtg actactcttg tctgatctcc
     5461 tgcgactgtt ccttctggtc ttatacccgg agttaagcaa acaaattcgt ctcctgccat
     5521 ttctttgatc gcttctactt cccaagcaga agaaaccaca ccatctaatc ctgctttttt
     5581 ggcacagcta gcataatgga tcacactttt ttctaaagaa acttcgatta gttgatcggc
     5641 atgcatttct tcttcacttg tagaagtaag ctgagtgata gctagaagta gcggacgttt
     5701 tccaccttct ggagtcccct cttctaatcc tcgcattgcc gcctccatca tacgaatacc
     5761 gccagcggca tgaacacagg tgagatcaac accaagcttt gccaatcccc gcattgcttt
     5821 ttcgaccgta ttcggaatat cgtgaagttt cagatctaaa aatacattat gaccagcctc
     5881 ttttagataa cgtacgattt caggtccttc ttggtagaac aattccattc cgattttcac
     5941 aaacaatttt tcttctttcg ggaaaaatcg aagaaagtct tctacctctt gcctagttga
     6001 aaaatctaat gcaataattg gtcgttggtc tagttgattc atcgttcacg ttcctctctc
     6061 acttctttga ttaatctttc cagcgattcg atgcctagtt cgctcattcg cacagggagc
     6121 tctttaatta gtttaggaca gatatacgga tcggtgaaat tcgcggtccc tatagctaca
     6181 gcacttgctc cagccataaa catctcaaga acatcatcca ctgtctgcac gccgcccatc
     6241 ccaatgattg gaaggctgga tatacttgct acttggtgaa tcaaacgaat agcgacaggc
     6301 ttgattgctg gtccggagag tccacctgtt tgattggcta aaatcggttt tcttgtctta
     6361 aggtcaatcc gcataccaag aagagtattg atcatcgtaa acccatctgc ccctccagct
     6421 tcgattgcct gagcgattgg aacgatgtct gttacatttg gggacaattt gacatagaca
     6481 ggaacagaag aaactttctt tactgcttgt gttaattgaa aagcaacatc agaatctgtt
     6541 ccaaaagcaa ttccgccatg tttgacattt gggcaagaaa tgttcagctc gattgctttc
     6601 acatttggtg cttcgccaat tttgctacaa acttctacat agtcttcttc acaagcacca
     6661 gcaacatttg cgatgatggg taactcattg tactcctcta attctggaag aagtttatgc
     6721 ataacaacgt ctagaccagg attttgcaaa ccaattgcat tcaacatgcc agatggtgtt
     6781 tccgccactc ttggcgtggg attgccaaag cgggcatttg gagtcgttgc cttgaccatg
     6841 atcgatccaa gctgattcaa atcatagtat tttccgtatt ctttgccaaa accgaaacag
     6901 ccgcttgccg gcatgatagg attttttaat tctaggccag gaagtttgac cgctaaagga
     6961 ttctcattca tataattacc tctcctattt taaaaatcgg tccttcgtca caaactttca
     7021 cactttttgt tcctgtttca tcttccggcg tatgacaaac gcaagcatag caagccccta
     7081 tcccacatgc catgcgggat tctaatgata actggacatt ttgatgcgaa gaaaacagct
     7141 gttccactgt cttcaataac ccgtttgaac cacatgcaaa aacagctgta ggttccgtct
     7201 tcgttcctaa caataaatta ccgacatttc cgtgcatacc gtatgttcca tcgtcagtag
     7261 cgattcttgt ttctcctaga cgattaaatt ctttttcata ataaacgact tcatgtgtgg
     7321 caaaacctaa aaaatgcgtt ggcttaattc ctttttcgac taactgtttt gataactcat
     7381 atagaggagg gacaccgatt cctccaccga ttataaatac ttcgtctcct gtatgaagat
     7441 tggacaaatc aaaaccattg cctaaaggac ccatgacatc taaatggtct ccggcagaaa
     7501 gagtagagaa gactctagtc ccctcacctt ccacccgata aatgatacgg caagtatttg
     7561 tttcgtggtc gatatgattc aagctaatcg gtcggcgtaa tagcaagtct gcacgcggca
     7621 cgagaatatg caagaactgt cctggttgct gcatttcttt tactagatca cctttcaatg
     7681 tcatttcaaa aatctttggt gccagctgtt tttgctgaac gatttccatc atttcttgtt
     7741 tcatctgtcc cctcctgcaa cggtttttcg tctttttgtc ttaacatgtg ctcgccgtta
     7801 aatagggtta gttgaaaaag cacgggattc catcactcga acgatcgcat ctgctgtgtc
     7861 taaagaagta aacaatggaa taccatgttc aactgcttct cttcggatga tgaaaccgtc
     7921 tttagaagca tttccccgat ctttatcaat cgtattgaca accacttgtg ttttaccatc
     7981 tcttattaag tcgactacat ttttttctgc tgcttcctca gaaattttcg ctactgtttc
     8041 aacgaccaga ccattttttt cgaaataagc agctgtatgt ttcgttccta tcagactata
     8101 gccaatttct gtaaaacgtt tagctaattc tagtgcttct tcttttgttt cgtccgcaat
     8161 cgtaaataat acggctccat attcaggcaa gtgtaatcca gaagcttcga atgccttgta
     8221 caaagctttg tctaattgtc tgtcagatcc cattacttct ccagtcgatt tcatttcagg
     8281 ccctaagtaa gtatcaactt tctgcaattt cgtaaaagaa aagacaggtg ctttaacatg
     8341 aacttctttt gtttctggat aaagtccgtt ttcataacca agtgtcttta gtttctctcc
     8401 caaaatagct ttcgtcgcaa tttgtgccat tggaattcct gtcaccttac ttaagaatgg
     8461 aacagtccga cttgctcgag gattgacttc gatcacataa acctcatttt catggatgac
     8521 aaactggata ttcatcatcc cgacacaatt caaacctaat gctaagcgga tcgtgtaatc
     8581 ttcgatcgtt ttcttgatcg attcagataa tgtttgaggt ggatacacag ccattgaatc
     8641 ccctgaatgg acaccagcac gctcaatatg ctccatgatc cctgggatga gtactgtttc
     8701 accgtcacaa atcgcatcca cttcgcattc accaccaagc aagtaacggt ccactagtac
     8761 tggatgttct ggagatgctt tcacagcatg ttccatgtaa tcttctaaat ctcgctggtt
     8821 ttcaacgatt tccatcgcac gtccgcctaa tacatagctt ggacgaacaa gaactggata
     8881 accaattctc gaagcgataa ccacggcttc ttctttactt gtagcagtat cccctaaagg
     8941 ttgaggaata tccaactctt tcagcgcttg ctcgaataaa tcacgatttt ccgcacgatc
     9001 caagtcttcg atcgttgtcc ctaagatttt tacaccagca gcagtcaaag gttcggctaa
     9061 gttgattgct gtctgaccac caaattgaac gattactcct atcggctgtt ctagctcgat
     9121 cacattcatc acatcttcga aggtcaatgg ctcaaaatat aatttatccg aaatagaaaa
     9181 gtcagtcgaa actgtttcag gattgctgtt catgatgatt gcttcgtatc ctgcctgctg
     9241 gattgctttg acagaatgca cagtcgcata gtcaaattca accccttgtc cgatacggat
     9301 aggaccagat cccagtacta gaattgaggg cttttctgat cgtgaacttt cattttcata
     9361 ttcataggaa ctataaaaat aaggtgtttt tgattcgaat tccgctgcac aagtgtcaac
     9421 catcttatat acaggaatca aatcattttc ttttctcatc tggcgaacgg cttcttcgtt
     9481 tatttcccac agctccgcca cttttttgtc tgtaaaaccg ttttgtttcg ctgttttcaa
     9541 tatcttgtca tccaacggct gctgtttcaa ctgctgttcg atttctacga tatgcaacag
     9601 tttatctaaa aagaataaat cgatttttgt caaatccgct aattcttcta tggtgtaccc
     9661 tcttctaata gcttcagcta cataaaacag gcgatcatct tgtgctcgaa ccactttttg
     9721 gattaggtca tcgtccgtga tgtctgccaa ctcaggaagt tcgatatggt gtgtaccgat
     9781 ttctaaagaa cgcacagctt tcaataaaga ctcttcgata ttccgaccga tagccatcac
     9841 ttcaccagtt gctttcatct gcgtgcctaa aagccgttct ccgttttcaa acttatcaaa
     9901 cggccagcga ggaattttag ctacaacata atctaatgca ggttcaaatt ctgcaaaagt
     9961 cgttcctgta acaggatttt tcatttcgtc taatgttaat ccaagcgcta ttttagcagc
    10021 tagcttagcg atcggatagc cagtcgcctt acttgctaac gcagatgaac gagaaacacg
    10081 cggattgact tcaatcacat aataattaaa actatgtgga tcaagagcga gttggacatt
    10141 acagcctccc tcgatcttaa gtgctcgaat aatcgaaaga gaagcatcac gaagcatttg
    10201 atattcatgg tcagacaatg tttgactagg ggcaaatacg atagaatcac ctgtatggat
    10261 acccaccggg tcaaaatttt ccatattgca gacaacgatt gcattatcag ccgaatctct
    10321 catcacttca tattcaattt ctttaaaacc tgcaatactt cgttcaatca aacattgggt
    10381 agcgggggaa agacttaatc cattttctga tacgatacgt aattcttctt cattttcaca
    10441 catacctcca ccagttccgc ctaaagtaaa ggctggacga acgatgacag gatagccgat
    10501 tttatttgca aaatctacag cttcttctac tgtattgacg atttcagact cggggattgg
    10561 ctgattcaat tctttcatca actgtttgaa tagatcacga tcttctgctt gatcgatcgc
    10621 agaaagcttt gttcctagaa gttctatgtt caattcttct aaaatacctg atgctgccag
    10681 ctccattgcc atattgaggc ctgtctgtcc gcctaaagtt ggaaggagag catctggacg
    10741 ctcttttcga agaatccttg atacaaattc aagtgtgatc ggttcgatat atacttgatc
    10801 tgccacttct ttatccgtca tgatcgtcgc aggattagaa tttactaaaa ccacttcata
    10861 tccttcttct tttaaggcaa ggcaggcttg tgttcctgcg tagtcaaatt ctgcggcttg
    10921 accaatgatg atcggaccag atccaatcac cataattttc ttgatatctg tacgtttcgg
    10981 catctgttta ctgctccttt cctgcgtcca tcatttccat aaattcatca aataaatgta
    11041 gtgcatcatg cggaccaggg gcagcatctg gatgaaactg cacactgaaa gcgggataat
    11101 ctctgtgttt gactccttct actgttccgt catttacttc tacatgagtt acgatgagtt
    11161 tttctggatc aatcgtggct tcatcgactg catagccatg attttgagaa gtaaaatcaa
    11221 ttcttccggt cgctacttct cgtaccgggt gatttaatcc tcgatgtccg aatttcattt
    11281 tataggtatc tgcaccgttg gctaaagcaa ataactggtg accgagacaa ataccaaaaa
    11341 tcggtacttt tccttggatt tttttgatca tttcgattgc ttctggcaca tcttttggat
    11401 caccaggtcc attcgtcagc atgactccgt ctggtgatag cgctaaaatc gtctcggcat
    11461 ctgtgttata gggaagtact gtgaggttac agtttctttt actcaattct cgtaaaatac
    11521 tatgtttcaa gccgaagtca atgacaatga ctttccgacc tgttcctgga cttggataag
    11581 gtttgttagt tgagacttgc tgcacttgat tggttgctag aacggttgct ttcagctgat
    11641 caaatgcatg ttcgaaggaa tctccagcat caactatact agctttcatc gttccgactt
    11701 cacggatttt gcgagtaagg gcgcgcgtat cgatacctga tattccagga attccttttt
    11761 gtttcaaaaa tgaatctagc gtcatttggt ttcgccagtt tgaagctaca cgtgcatgtt
    11821 cttttactac tactcctttg catgttggag aaatcgattc ataatcatct ctatttaccc
    11881 cataattacc caccatagga taggtaaatg taatgatttg accgttaaaa ctttgatcag
    11941 tgatagtttc ttgataacca gtcatacttg ttgtaaaaac gatttctccg aagacactag
    12001 catctgcacc aaaagccttt ccttcgaaaa ctgttccatc ttctaatata agcaaacgtt
    12061 ccatactttt attctccctt ctgccacaca agttttccgt tgacaaaagt catttgtgtt
    12121 tcgcctttta ctttccagcc gataaaaggt gtattctccc cttttgataa aaagtcttct
    12181 ttatcgatcg tgcaagtttg tgtaatatca aaaactgcta catcagcggg ggcaccaacc
    12241 gttagcgttc ctgcattcaa accaaaaatc tcagccggtt taactgccaa ccaattgatc
    12301 acttgttcca acgtgaatcg accagtttct acaaaatgag tatagatcag ttgaaacgct
    12361 gtttcgcttc ctacgatacc aaatggtgat ttcatgaaac tttggctttt ttcttctaat
    12421 ccatgggggg catggtctgt agcgatgcaa tcgatcgtac cgtcaagcaa gccttcgatc
    12481 aaagcttctc tgtcctcccg tcctcttagc ggcgggttca ttttccaaaa gccgaaatct
    12541 tcgggaatat cttcgtcgat caaaataaga tgatggggtg aaacttctgc tgttacgtgg
    12601 attcctgctt tttttgcgtc tctgattaca cggacacttt ctttagtaga aacatggcaa
    12661 acatggtaat gaacaccagt tgcttcagct agcaacagat ctctagcaat ttgagaagac
    12721 tcggttacgc ttaagatccc gggaagacca agttcttctg ccttcttgcc agcgtgcata
    12781 acgccgccaa ataataaaga ttcatcttct gtatgggcta ctagggcttt attgtttttt
    12841 gcagcttctt tcatagccag atacatcgtc ccagctgttt ggacacctac accgtcattt
    12901 gtgaaagcaa aagcaccttc ttcaatcagt gcttcttgat caactaattt ctcacttcgc
    12961 agattttctg tgattggcgc atactgcaat accttcacaa ctgcatcttt tctaatgatg
    13021 tcatatactt gtctcagctt ttctgccgta tcaggtacag gatttaaatt cggcatcgcg
    13081 catacagtag tgaaaccgcc acgagcagct gagcgagtcc cagcttcgat cgtctccttg
    13141 tatgtaaatc ctggctctct caaatgaaca tggacgtcca ctaaaccagg tgtgatcaat
    13201 tgtccctttg catcaaacac ttcatcaaat tcggcttctg agaatcctgt accgattgct
    13261 ttgattcgcc catcttctat ccaaatttct gctggtgtcg tcatattttt tcgtgtattg
    13321 atctgaccgt ttttaatgag tgttttcatt ttttcatcct cctataatta cgcttttcca
    13381 tttagcactg cttctaagat tgccattctg acgaatactc cattgctcat ttgctgaacg
    13441 atccgcgact gtaatccttc cactaatgaa tcagctaatt ctacatcacg gttaactggt
    13501 gctgggtgca taatgattgc atgtttttgc aacttcttcg cacgctcaac tgttaaccca
    13561 tgttcctgat ggtagccttc ttttgagaaa ctttcttttc catcatggcg ttcgtgttgc
    13621 acccgaagca tcatcatgac atcaacttga tctagtagtt catccaatgg catataatgt
    13681 ccgtacactt cgaattcatc gtcgtaccat tctctaggac ctgagaagaa tacttgtgcg
    13741 cctagacgtt ttaacatctg catattggat ttagctacgc gagagtgagt gatatcacct
    13801 acgatggcaa ctttcagacc ttcaaaatga ccaaattctt cataaatggt catcaaatca
    13861 agcaagcatt gggttggatg ctgtccgctt ccgtctcctc cattgataat cgaacactgg
    13921 attgttttac tttgtatcag ttcgtcataa tagttctcat caccatgacg gataaccgct
    13981 acgtctacgc ctaatgctga catcgtcagt actgtatcgt atagtgtttc ccctttttgg
    14041 acagagcttg tgcttgcttc aaattcaatc acttctagcc ctagtttttt ctccgctaca
    14101 tcgaagcttt tatgtgtgcg agtactattt tcgaaaaaga ggtttgtagc aaagtactgg
    14161 cgcttttgcg gagtccaatt agcaccttgc ttgaattcct gccctctgcg gatcaagccc
    14221 ataacttctt gatcagtaag tgcctcaacg gtcaataaat gctttaagct gatacgttct
    14281 gatttgatga tcataatgtt tcctccttag ttttcttcgc ttcgtgaagt ttctggtaag
    14341 ataagattta aaatgatgcc aagtacagtt gctaatgcca ttgccgacaa tgtaaatgtg
    14401 cctacttcaa agacgagtcc gccaattcct actactaaga taactgaagc aatcaacaga
    14461 tttttctttt tatcaaaatc gattttgttc tcgattaaaa tcttcattcc acttgcggca
    14521 attactccaa ataagataaa actgattcct gagatgactg gtccagggat acttaaaatc
    14581 aaagcgctta attttcctac gaatcctaaa atgactgcaa atactgcagc tccaccgatc
    14641 acaaacacgc tatggacacg tgtgatagcc agaacaccaa tgttttcacc gtaacttgtt
    14701 acaggaggac ctccgacaaa ggctgccacg atttgtgcta atccatcacc tgtcaacgtc
    14761 ttatgaagac ccggttcttc aaagaaatta cgtttagtta gtttattcaa taccataaga
    14821 tggccgatat gctctgtcat cgtcacaaaa gcgatgggtg ccattgttgt gattgctcca
    14881 agatatagct ttggttcata ttgtacaaag ggaatctcaa agttaggaac agcaaaccag
    14941 gcagcgtcta cgattggctt agtatccacg attccaaaca gcaaggctac gatgtatccg
    15001 ctaataatac ctagtaaaat cggaatcaat ccgagaaaac ctcttagcca catgttgaag
    15061 aaaattgtta atcctaatgt gatcagcgct acagcaatat acttgaaatc atacgtgccc
    15121 tgattataca tagcatttgt tgctgcattt gccgcaagtc ccaacccgat aaccatgatg
    15181 actggaccga cgacgattgg aggcaagatt ttatccagcc atgctgaacc gatctttttg
    15241 atgatcagcg caacaatcaa atagactaat ccagttgtga ctgctccttg tgcaatcgct
    15301 ggataaccat ctgttttcat caaaagctgc atcgctgcaa tgaaagcgaa gctgcttcct
    15361 aagtacgcag gtatctttcc tttagtagta aataagtaca ccaatgtacc tagtcctgaa
    15421 ctgactagcg caatacctgg atcgatacct actaagatgg ggactaacac ggttgctccg
    15481 aacatcgtga acaagtgttg taaacttaat cctacccaat gtcctggggt tgggcgatcg
    15541 tggatatcta acaccacgtc atcattatga aatccttttt tctccgacat gtttctctct
    15601 cctctaatct tcctctttca aaatcatgat gcgatcttgt ccatcaagct cttgcatctc
    15661 tactaaaatc tcttccgccc gagatgttgg aatgttcttc ccaacataat ctgcacggat
    15721 tggcaattct cgatgcccac gatcgaccag gactgctaat gaaatttttc ttggtctgcc
    15781 aaaatccatt actgcgtcca ttgctgctcg aatcgttcgt cctgtataca aaacatcatc
    15841 aatcaaaata acttcttttc cttctaagga aactgggatg tcagaagaat gaagttccgg
    15901 ttcttctgga ttttcttttt tgtcatctcg gtataaagtg atatccagtt ctcctacagg
    15961 aatatcgata tcttctagct gcttcaggcg ttcggcaatt cttgatgcaa tgtagattcc
    16021 tcgtgttttg atacctacca atacgatatc ttggatacta tggtttctct cgataatctc
    16081 ataagtaatt cgagtcaatg ctcgcttcat ggttacctga tctactactt cttttgcttg
    16141 cattttcatt ccccctactt ttttgcataa aaaaactcct gccctaagag gacaggagga
    16201 atgtttagac tgagcgtata ctgatacact gatagctata ctatgtcaat gagcaatcta
    16261 cttatggcgc accatacgta tccttgctcc cacccttctt agcctcacgg gactaaactt
    16321 aaaggaattc tattaagttt tatgctttcg ttgttaatta ctataccgtt atctaaatta
    16381 aaatgcaatg ttttttctta atttttctaa agttttttca aaaacttctg gaagaggtgc
    16441 ttcaaagatc atcttttcgt ttgtagtcgg gtgcgtaaaa cctaacattt gcgcatggag
    16501 aaactgacca ttgcctttca aagttttctt cggtccgtat actggatctc cagctaacgg
    16561 ataaccgata tacttcatat gcacacggat ctggtgtgtt cttccggttt ctagttttaa
    16621 ttcaaccaga gtaaatcctt cgaaacgttc caatacagta aaatgtgtga cagccgactt
    16681 cccatcttct cttatcgcct gcattttccg gttttccttc gaacggccga ttggcgcatt
    16741 gatggttcct ttttcgtgag gaatctctcc atggactaaa gcaacatatt tgcgcaaaga
    16801 ggtcttatct tttaattgct gagctaatga ttcgtgtgcc tggtcatttt tggcaaccat
    16861 tagcagtccg gaagtatctt tgtcgattcg gtgaacaata cctggacgaa taacgccgtt
    16921 gatcgtagaa agatccttga tatggtacat cagcgcattg accattgtgc catgcgtatg
    16981 acctgctgaa ggatgaacga ccattccttg aggtttgttt atcaccgcta catcttcatc
    17041 ttcgtaaacg atcgtcaacg ggatattttc tgggaccaca tctaatgttt ctggttcggg
    17101 gatcaagaca gtaatctcgt ctccttgttt tactttgtaa tttgctttga ctggctgtcc
    17161 attgacagaa accgctccat ttttcagcca cagctgaatt tgcgaacgac tgtaatcggt
    17221 gaataaatta gtcaatactt tatcgatcct accagattca tggtgaatcg tcgttttaag
    17281 ttctgtcatt tttttctttg atccttttca tctaaaataa tataaattaa catacaaatc
    17341 actccacata caagtgtcac atctgctacg ttaaaaatcg gaaagctcat aaaatctgtc
    17401 tggaacatat ctactacata gccaagacga atacggtcga taaaattgcc aatcgcacca
    17461 gctagaatca aactcaatcc aatcatgaac cagcgattac ctttttcacg atttttaatc
    17521 atcaatgtga gaatcacaac gactgcaatg agtgtcacta tagtaaagaa ccacatcttc
    17581 ccttctagca tactccatgc ggctccagtg tttcgaatat gattcagtga caaaatacct
    17641 ggtataagat ttttcgtctc tccaagcgct agattatcag tgatccacca tttgaccaac
    17701 tgatccactc ctactagcaa agcgctgaat atccaataaa taattaacaa aaaatcaatc
    17761 cttttctatt tgtttataaa tatcacgagg tcgtacgatc ccgagcagat taccatcggg
    17821 attcccatct ttagtgataa gtacggcttc caaagtccct tgctcacgaa acatcgcttc
    17881 aacctcgtaa atatgtgttt tagctggaac aaaatgataa tttgtgtact ttccgttttg
    17941 gatgatcaaa tccaacgctc gtttatttgt taaatcaacc ggaccttttt ggctttcttt
    18001 tgctagccaa aacccgatat tacgtaaagt aatcaatgct acaaattttc ctttattgta
    18061 taacggaaat tgtgaatacc ttttttctgc tactatcttt aacagttcaa gtaacggaag
    18121 atcttgttca aaacctgtta cacgttttgc aaatcgaggc agcacggttt caggctgaat
    18181 cagttcttgt tcgatcagct ggattcgctt gactgtccaa tcatttggct ctgcaatgac
    18241 aaaatcgact gcgatccgat catggacgat cgcattcctt aactgagcaa gctgtaaaag
    18301 atcatcttcg tattttctga tcattaattg ttttctttga gctaggcgtc tgacaagttc
    18361 ggtaaatccc atatttcgtg catttcccat ctcgtctctc atccattttt cgattcgatt
    18421 gaaactgatt aaaaaaagat cagagtttgc cataattttc ctccttgatc aaatatgccc
    18481 ggttttttat atagaaaaag aacgcaatca acacaataac tgcaaaaaaa ctgaaaaata
    18541 aattttgtag gaaaacggcc cccaacccag ttgctagaaa taaaaacgga tagatttgaa
    18601 gaatcaagta attcattgtg atcacataag caggctttgc ttctttcact gtataaacgg
    18661 ggctttttag ccaataaaag gtgacctgca tcgtaaaggg cacttccaga tcaagcgtta
    18721 tcttttgttc atgactgaga acactgcgtt tttcattgtt gattcttagc tgaatcgagc
    18781 tacccatacc gtaaaaaccg gtttttctta taaatgtcgt tttcatcata ccctcctatt
    18841 atgctttagt atagctcaaa atacaataaa gaaaaagaat gatcctagtg taaacaaaat
    18901 taattcccta tcgtctaaaa aaagaagctg tagtcaaaat acagctcctt cgatggtaga
    18961 ttataatcaa gcttaaaaca atcccaacac ttctccgtca tttgtcacgt ccatattcaa
    19021 tgcagcaggt tttttaggca aaccaggcat cgtcataaca tctccagtca aggctactaa
    19081 aaatcctgcc cctaattttg ggatgatttc gcggatcgtg atggtgaaat cttccggtgc
    19141 acctagcagt gaaggatcat cagaaaatga atactgtgtt tttgccatac aaatcggcag
    19201 tttgtcccat ccattcttcg taaactcttc tatttgtttt tgcgcttttt tgctgaaaac
    19261 aacaccattt cctccgtaaa ttttcttgac gattgtttct gtcttttctt gtatcgtttc
    19321 ttctaggcgg tataatggct tgtaattagc ttcttttcta tcaatcaatc gtactacagc
    19381 ttttgctaaa tcaacgccgc cttctgctcc atctgcccat acactcgctc tcttggcaag
    19441 aatcccctga ttttcacaca agtgctccaa cagtgtgagt tcattgtctg tatctgttac
    19501 gaattcattg attgcgacga tcacaggtag ctgatattgt tccatattac gtatatggcg
    19561 ctttaaattt gcaaaaccta ctttcaaagc atcaagattc tcatttttaa gttcatcttt
    19621 tttcattccg ccatgcattt tcaatgcacg gacagtggcg acaatcacca cagcatccgg
    19681 cgcttttttc aagttaggta ctttgatatc taaaaatttc tctccgccaa gatctgcacc
    19741 aaatcctgcc tctgtgacaa cgtaatctgc taatctcaat gctgtcttgg tagctaaaat
    19801 actattgcag ccatgagcga tattggcaaa cggtcccccg tggacaaaag ctggtgttcc
    19861 gtaaattgtt tgtacaagat tcggcttgat tgcatctttc agcagtaatg ccagagcgcc
    19921 ttctgctttc aagtctccag cagtcacagg ctgacggtca aatgtatagc cgacaacgat
    19981 ccttgcaaga cgagccttta aatcttcaag gtctgccgca agacacaaga tagccatgat
    20041 ttcgctggca actgtgatat caaacccatc ttctcttgga acaccttgga ttggcccacc
    20101 caagccaaca attactttac gcagttcacg atcattgaga tcgactaccc gtttccaaat
    20161 cactcggcgg gcatcgatgt tcaattcatt tccttgatga atatgattat ctaaaagagc
    20221 agataatgca ttattagctg ttgtgatagc atgcatgtca ccagtaaaat gcaagttgat
    20281 atcttccata ggcaatacct gtgcatatcc tccgcctgca gcaccacctt tgattcccat
    20341 cacaggacca agtgagggtt ctcgcaatgc aataacagat tttttattga ttttattgag
    20401 cgcgtctcct aaccctacag tgatcgttga ctttccttcg ccagctggtg ttggattgat
    20461 ggaagtaact aagatcaaat gcccctcagc gttatccttt gatcgattga ttccagaaaa
    20521 attgattttc gccttatatt tcccatacag ttccaaatcg tcttccttaa gatctaattt
    20581 actagccact tcaacaatcg gttctagctc tgtttcttgt gaaatttgca gatcattctt
    20641 catttgatta ccccttttca cgaactttat tcaacaaaat atcttctttg ttaagatatg
    20701 ccaattatac ctttttttat tcagaaaaaa aacataaaat tacaggtttt taatttattt
    20761 gacgtactta taaatactct ttataatgaa ggctatgatc agactttaga aaggtcgtga
    20821 gtgctttgac cacaggaatt gtaaaatggt ttgacaacaa aaaaggatac ggctttatca
    20881 gttatgatga cacagaagaa atttttgtac attttactgc gattgaagaa gagggattca
    20941 aaactttgga agaaaatcaa gtggtcgaat ttgagatcat cgaagggaat cgaggaacac
    21001 aagctgccca tgtcaagaaa ggcaacgttc agactaccga tactagtatg catgaataga
    21061 taaacaaata gagtgagacg ctaaacggga tgaaccgtac gtctcactct tttcttttcc
    21121 tgtaaaaatt cgttttttta ggttctgatt tttaaaatta gctttttctt tttttcgtat
    21181 aaggataagg aattgattct gatttgggtc cgtttttttc aaaatgatca aaaaggacta
    21241 cttgatcttt ttttcgcatc atgaaaacag atggggcttt ctcatcttgc aacatcattt
    21301 gcaccccagt attagttggc gtcaaagatc gaatctgtcc gacagatatt tctcggtctt
    21361 tccaaaaagc agcataacgt ataatcacag agtttgattt taaaataaaa gaacgccgca
    21421 aacctagaaa tagaaaaaca agaaaaatac ctaaaacaaa attgcttttc caataaggac
    21481 ctgtgttttc taaagaaaga atcagactaa aaaacagaat gatcaaggtc aatgaccaat
    21541 agatgatcgt atgtgccagt tcaggctgcc atcgaatatt ttttttcatt ctctaacctc
    21601 tcaccgattc agtaatgata taatcttacc ataacattag tttgaacaaa aaggagaatc
    21661 tctttttatg ttaaaagtat acattgatgc ctctacaaaa ggcaaccctg gtcctagtgg
    21721 aggtggaatc ttgattgtct atcaaggtaa acaagaacag ctatctatcc cgctttctat
    21781 agggacaaac catcaagcag agttcgaagt ctttttaaaa actttagaat ttctaaagaa
    21841 aaacgactta cagaatgaaa cgattttctg tttttcagac agcaaaacac tagtttctac
    21901 ggtcgacaaa aacgcaacga agaacgagct cttccttccc taccttcaaa gaatccaaga
    21961 acttctatcc gaattttctt tattgatcct gcaatggttg cccgaaaaca aaaataaagg
    22021 agcagataat cttgcacgtc aggctctcca taaacaatta aaagaaaaaa agactcttta
    22081 aattaaaaac tttaaggagt ctttt
//
LOCUS       DAZUMK010000037        21838 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000037 DAZUMK010000000
VERSION     DAZUMK010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.037"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..408
                     /locus_tag="ACG808_001346"
     CDS             133..408
                     /locus_tag="ACG808_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFS5046979.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            414..1181
                     /locus_tag="ACG808_001347"
     CDS             414..1181
                     /locus_tag="ACG808_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFS5046980.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            1349..3178
                     /gene="typA"
                     /locus_tag="ACG808_001348"
     CDS             1349..3178
                     /gene="typA"
                     /locus_tag="ACG808_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFS5046981.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            3439..5211
                     /gene="recQ"
                     /locus_tag="ACG808_001349"
     CDS             3439..5211
                     /gene="recQ"
                     /locus_tag="ACG808_001349"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFS5046982.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLRKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            5325..5627
                     /locus_tag="ACG808_001350"
     CDS             5325..5627
                     /locus_tag="ACG808_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFS5046983.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            5630..6793
                     /locus_tag="ACG808_001351"
     CDS             5630..6793
                     /locus_tag="ACG808_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFS5046984.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            6819..10247
                     /locus_tag="ACG808_001352"
     CDS             6819..10247
                     /locus_tag="ACG808_001352"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFS5046985.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            10268..11392
                     /locus_tag="ACG808_001353"
     CDS             10268..11392
                     /locus_tag="ACG808_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFS5046986.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            11373..11819
                     /locus_tag="ACG808_001354"
     CDS             11373..11819
                     /locus_tag="ACG808_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFS5046987.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            11803..12141
                     /locus_tag="ACG808_001355"
     CDS             11803..12141
                     /locus_tag="ACG808_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFS5046988.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            12260..12820
                     /gene="rsmD"
                     /locus_tag="ACG808_001356"
     CDS             12260..12820
                     /gene="rsmD"
                     /locus_tag="ACG808_001356"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFS5046989.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            12817..13308
                     /gene="coaD"
                     /locus_tag="ACG808_001357"
     CDS             12817..13308
                     /gene="coaD"
                     /locus_tag="ACG808_001357"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFS5046990.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            13298..14341
                     /locus_tag="ACG808_001358"
     CDS             13298..14341
                     /locus_tag="ACG808_001358"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFS5046991.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            14500..15228
                     /locus_tag="ACG808_001359"
     CDS             14500..15228
                     /locus_tag="ACG808_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFS5046992.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            15344..15589
                     /locus_tag="ACG808_001360"
     CDS             15344..15589
                     /locus_tag="ACG808_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFS5046993.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            15586..15804
                     /locus_tag="ACG808_001361"
     CDS             15586..15804
                     /locus_tag="ACG808_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFS5046994.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            15940..16479
                     /locus_tag="ACG808_001362"
     CDS             15940..16479
                     /locus_tag="ACG808_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFS5046995.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            16472..18745
                     /locus_tag="ACG808_001363"
     CDS             16472..18745
                     /locus_tag="ACG808_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFS5046996.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            18783..19829
                     /gene="holA"
                     /locus_tag="ACG808_001364"
     CDS             18783..19829
                     /gene="holA"
                     /locus_tag="ACG808_001364"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFS5046997.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(19918..20172)
                     /gene="rpsT"
                     /locus_tag="ACG808_001365"
     CDS             complement(19918..20172)
                     /gene="rpsT"
                     /locus_tag="ACG808_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFS5046998.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(20572..20841)
                     /locus_tag="ACG808_001366"
     CDS             complement(20572..20841)
                     /locus_tag="ACG808_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5046999.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(21103..21231)
                     /locus_tag="ACG808_001367"
     CDS             complement(21103..21231)
                     /locus_tag="ACG808_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047000.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(21311..21766)
                     /locus_tag="ACG808_001368"
     CDS             complement(21311..21766)
                     /locus_tag="ACG808_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFS5047001.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
BASE COUNT         7322 a         3711 c         4604 g         6201 t
ORIGIN      
        1 aaaatcagtt gcgttttgtt ttttagaatt atcaataata ttattatgta aacttaaaaa
       61 tatgacaact tcttggttta ttaccacttc ctatgctaca ctgaagagga atatgaaata
      121 ggagagttga agatgcaaga atatcagtat ccacttgatt tagattggac aacagacgaa
      181 atggtcattg taatgaagat gtgggaagat ctggaacgtg cgaatgaaaa agggataaac
      241 aatgaagctt ttttgaagac ttatcagcag tttaaaactg ttgtcaaatc cattggtgag
      301 gaaaaacgat tgggacggga atttgaaaaa gcttccggtt attctttata tcgtacagtg
      361 cagcaagcaa aaaaaaatcc aaacaaaact ttgagaatga acgggtgaga taaatggaac
      421 agatgattga tcatatcaag tcctggcttg aagaagctgg tgagaaaatc aaatcttctc
      481 aacaaacatt aacaatctcg caaaaatcaa atcgaacgga tctagtaaca aatgtcgatc
      541 gaaacatcca gaattttcta atcgaaaaaa tccattcctt tgatccagag gcgaaaatat
      601 tggcagaaga agaagggtgt aatcaattat cggatctcga tggaagagtc tttatcatcg
      661 atcctattga tggcaccttg aattttgtga ttgaagggga aaatttttgt attatgctcg
      721 ctgtttatga atccggcgtt ggaaagcttg gtttcattta tgacgtgatg agaaatgaac
      781 ttttttgggg tggaaaagga ataggcgtgt ataaaaatga tgagccattg cctagaccag
      841 caaataaatc cttatcggaa ggcttacttg gtgtgaatag ttatcttttc ggtcataata
      901 aatatggaat ccgagaaatt gcggaaagca gcatggggac acgtatgtac ggatgcgctg
      961 gtcttgaact agtggcaatc ttgaaaggaa accacattgc ttatatttca aaccttagcc
     1021 cttgggatta tgcagcggga aatgtcttat tggaagagtt tggtatggta acaagcggtc
     1081 tttttggtac tcctttgaca tttgaaaaaa gggaatacta tttagcaggg acttcttgtg
     1141 catatgcaga aatcgcagac cttttgaaaa aagaaggctg aaaaaaaggt agaaatcatt
     1201 cacaaaaatg ataaaaacga gcaaaagatt aattttctcg aaaaaatgaa aacattttac
     1261 tatatttcat gaaaaaagtc tgctataatt agcagtcgag taaaatttgc cctgtcataa
     1321 agaaccaata aaaaaggagt tagttacatt gaattataga aacgatatcc gtaacgtcgc
     1381 aattattgcc cacgttgacc acggaaaaac aacattagtt gatgaactat taaaacaatc
     1441 agatacatta gacgcacata cgcaattgca agaacgtgcg atggactcaa atgcgctaga
     1501 aaaagaacgt ggtatcacga ttttagccaa aaacacagct gtagactata aaggtattcg
     1561 cgtaaacatc atggatactc caggacacgc ggacttcggt ggcgaagtag aacggatcat
     1621 gaaaatggtt gacggcgttg tgttggtcgt agatgcgtat gaaggaacaa tgcctcaaac
     1681 acgttttgta ttgaaaaaag cactggaaca acatatcaca ccaatcgttg tcgtaaacaa
     1741 gattgacaaa ccttctgctc gtcctgagca tgtcgtagat gaagttcttg aactattcat
     1801 cgaattagga gcagatgatg atcagttaga ctttcctgtg atttatgcat ctgcactaaa
     1861 tggtacgtca agcttgtcag atgatccagc agatcaagaa ccaaccatgg cacctatttt
     1921 tgatacaatt attgaaaaaa tccctgctcc tgtcgataac agcgacgaac cattacaatt
     1981 ccaagtttca ttacttgact acaacgatta tgtaggtcgt atcggaatcg gacgtgtatt
     2041 tagaggaaca atcaaagtag gagaccaagt agcattaatt aaattagacg gaacagttaa
     2101 gaaattccgt gtaacgaaat tatttggttt ctttggacta aaacgtctag agattcaaga
     2161 agcaaaagct ggagacttga ttgctgtctc aggtatggaa gatatctttg tcggtgaaac
     2221 agttactcct gtggatcatc aagatgcgtt acctatcctt cacattgacg aaccaacatt
     2281 gcaaatgact ttcttagtta ataattcacc atttgccggt cgagaaggta aattcgtaac
     2341 cgctcgtaag attgaagaac gtttgatggc agaacttcaa acagatgtat ccttacgagt
     2401 tgaaccaaca aactctccag atgcatggac agtttctggt cgtggagaat tgcatttgtc
     2461 tatcttgatt gaaaacatgc gccgtgaggg atacgaattg caagtatctc gtccagaagt
     2521 tattgaaaaa gaaatcgacg gtgtaaaatg cgaaccgttt gaacgcgtgc aaatcgatac
     2581 accagaagaa tatatgggta gtgttatcga gtccttgagc ttacgtaaag gtgaaatgca
     2641 agatatggtc catacaggaa acggacaaat ccgtttgaca ttcttaacac ctgcccgcgg
     2701 tttgattggt tattcaactg aattcctttc aatgacacgt ggttacggaa tcatgaacca
     2761 tacttttgac caatatctgc caatgcttcc tggtcaaatt ggcggacgtc accaaggggc
     2821 gcttgtttcg atcgatacag gaaaagcaac gacttattct atcatgagta tcgaagaacg
     2881 cggtacagtc ttcgtagaac caggtacaga agtatatgaa ggaatgatta tcggggaaaa
     2941 cagccgtgat aatgatttga ctgtcaacat cacgaaagca aaacaaatga caaacgtacg
     3001 gtctgctact aaagaccaaa catctgtcat caaaaaacca aaacaattga cgttggaaga
     3061 atctttggaa ttcttgaatg atgatgaata ttgcgaagta acaccagaaa gcattcgctt
     3121 gagaaaacaa attttagaga aaaatgctcg tgaaaaagca tctaaaaaga aaaaataatc
     3181 tgtaaaagga tgaagagaga aaccagcttg ttataaaaaa tagctggttt ctttttttgt
     3241 ccaactcttt tctgtggaga agtcatcttt gtcaatggac ataaattcag aaaatacgcc
     3301 gaaagacgtt taaaagactt ttaaaaaact tatttctaga gagtttttgt ttcataataa
     3361 gataatgttt cacgaaagga gatcaacttg tccttcgaag aaaatcgaag taaaatggca
     3421 agagaaagga gcgggaagat gaacgactta ttaaaacaat actttggcta tgatgagttt
     3481 agacctggtc aaaaagaaat tatccagaaa gtcattgatc aggaaaatgt attaggaatc
     3541 atgcctaccg gaagtgggaa atcaatctgt taccagcttc cagctttact attagatggg
     3601 cttactgtgg ttgtgtcccc tttaatctct ttgatgaagg atcaggtgga cgcggcgaat
     3661 caattaggta tacccgccac ttttatcaat agctcgttag atggttacga aacagctaga
     3721 cgcttccaag aaatcgatag acagcagtac cgtttacttt atatcgctcc tgaacgtttt
     3781 atcatgccgg attttatcca agcaatgaag cgatggaacg tccgtatgat cgctattgac
     3841 gaggcacatt gtatctctca gtgggggcat gatttccgcc caagttattt acagatggcg
     3901 aaccagctag atcaactgcc aaatcgtcct gtaattgttg ctttgacagc tacagcgact
     3961 gtgcaagtag ctgctgacat aaaaagactt ttaaaaattc cagaaaacaa ccatattcag
     4021 actggttttg aacgagaaaa tctacgattt caagtaatta aagatcaaaa aaaagaacaa
     4081 tatctaatcg aatatttaaa gatcaacaaa aatcaatctg gtatcattta tgcagcaacg
     4141 agaaaagaag tagaccgtct ctatcatttg cttagaaaat tcgatttttc cgtgggtaga
     4201 tatcatggtg gtctaaatga gaatgagcga acagaaatgc aagaagcctt tttatacgat
     4261 cgcttgcagc tgattgtagc gacaaatgca tttggtatgg gtataaataa aagcaatgtc
     4321 cgttttgtta tccattatca aattcctggc tctctagaag catactatca ggaagctggt
     4381 cgggcgggaa gagatggtct ttccagtgaa gccatcctgc ttttctctcc tcaagatatc
     4441 caagtccaaa agttttttat ccagcagtcc caaagagaag aagggcaaaa gcaaaaagaa
     4501 tatgaaaaat taaaagcgat gacagaatac gtgtatattg agtcctgtct tcagcaatac
     4561 atcctaaact attttggcga gacaagcagt ccgtgcaacc gttgtggtaa ctgtttagat
     4621 gacagagaaa tagtagaagt aacgacagaa gcacagatgg ttctttcttg cttaaaacgt
     4681 atgggtgaga actatggtaa acaaatgctg atgaaagtct tagcaggatc gaaagaacaa
     4741 aagctgcaag ctttaggttt tggacattta tctacctatg gactgatgaa aaaccaatcg
     4801 caaaaagaaa cgatgcagtt aattgaatat ttgatatcga atggctatct tctgaccatc
     4861 aatggtgagt atcctgtttt gaaagtaaca gaaagaggga tacaagtatt gaaaggacaa
     4921 gaatctgttt atcgaaagga acccaagaaa gtccagcagc tatcggatga agaaaccgat
     4981 acactatttg aagtgctgcg ggaactgcga acggatctag catcagaagc aggcgttccc
     5041 ccgtatgtcg tattttcaga ttccacattg aaagaaatga gccgaatacg tccatcaagc
     5101 agattggaga tgcttcagat aaaaggagtt ggccaaagca agcttgataa atatggcgaa
     5161 gcctttttat ctcggataaa aaatgcggac tctaacgttc tggataagta gaaagaacga
     5221 atacgtaaaa atgaataaaa acaactggtt aaatctgttt tttttgctcc ttatgaaaat
     5281 caaatatgtt ataattaagg aattagaatg ggaggtaagc acagatggat tctttgtcga
     5341 aaaaacttgc gatccaagta ttaaatgatg aagcagaacg gatcaaacga ttaatccgaa
     5401 atcaaaacaa cagtctctgt atctcacaat gcaaagcctt cgaagaagtg gtagatacac
     5461 agatgtacgg cttttcccgg caagtcggtt ttgctgtccg tataggagta atcggaaaga
     5521 acgagggcca gctgatgtta agtgagctag aacgagaatt gaatcagtta tatagtgagg
     5581 tttaccaaga aatttatgat aaaaaagaag ttggcaagga ggaatagact tgcctaagaa
     5641 agtgaaaaaa aggcatctat tagactatag catcttgatt ccctatttaa ttttatgtgt
     5701 attgggattg atcatggtat atagttctac gtcgtatcta ttattagaaa acggacaaaa
     5761 tccgtccgca tcagtcatta accagagtat tttttgggtg cttagcttga ttgctatcgc
     5821 actactttat aaaatgaaaa cagatgtttt gaaaaaccag cgtttgatca tggcagccat
     5881 cgcagttttg acgattttgc tgctgattgt tgtgtttttt ggtaaggaga tcaatggggc
     5941 gaaaggttgg ctgcaaattg caggattctc gatccaacct gccgaatacc taaaaatcat
     6001 ttctatctgg tacctgtctc tgactttatc taaaagacaa aacagtgtac aaaaagattt
     6061 tcttgggact gtgaaacgtc cacttgcaat ggttatcgga cttactgcat tagtcgctat
     6121 tcttcccgat tttggtaatg cggcagttat ctttttgatt attttggttc tgcttttagc
     6181 cagcggagtc aattatgtgt atactttgat tgtaggagta ggaggctttt gtctaagtac
     6241 ttttacgatc tggctgatca atatcacaaa cggaaagatc ttacccggac gtttacagta
     6301 tatctataat cgatttgcta tttaccaaaa tcctttctca gacgaattaa ataaagggca
     6361 tcaactagtc aacggctact atgccatgtt caacggagga ctatttggca ggggattagg
     6421 taacagtatc cagaaaaaag gatttttaca agaagcacaa accgatttta tttatgcaat
     6481 cgtagtggaa gaactgggag tgattatggg gattttgata ttagcgttgc tattctttat
     6541 gattgtgcgc attgtccttg taggtatccg ttcaaaagat ccgttcaatt cgcttttgtg
     6601 cattgggatc ggcgcaatgt ttttgatcca agtatttgtc aatcttggag ggattactgg
     6661 cgttattcct ttgacaggga tcacgttccc atttttaagt caaggaggct ccagtttgct
     6721 gatgctttcg atttgtgtcg gctttgtcct caacatcagt gcggatgaaa aacgcaagag
     6781 cttaggttta taataaaaaa agatgtagga gtggttcgat gaaaaaagtt ttagttgcta
     6841 acaggggtga aatcgctgta cgtgtatttc gtgcttgtac agaattagga attaaaactg
     6901 ttggaatcta tgcagaagaa gatgaatatt ccgtacaccg tttcaaagcg gatgaagcat
     6961 atttagtagg acaaggaaaa aaaccgattg atgcgtatct agatattgaa gggatcattt
     7021 ctatcgcgaa agaatgcgga gctgacgcta ttcatccggg ttacggtctt ttgtcggaaa
     7081 acttgaattt tgcgcaacgg tgcgaagaag aaggcatcat ttttgttgga cccaaactgc
     7141 atcatttaga tatttttgga gataaaatca aagcaaaagc tgcagctatc gaagcgggga
     7201 ttgcttcgat tccaggcaca gatggaccaa tcgcttctat agacgatgcc ttggagtttg
     7261 cgaaacaata cggttatccg attatgatca aggcagctct aggcggcggc ggacgcggca
     7321 tgcgtgtagc tcacgatgaa aagagtgcaa gagagggcta cgaacgtgcg aaaagcgaag
     7381 caaaagcagc ctttggtagt gatgaagtgt atgttgaaaa atacatcgct aatcctaagc
     7441 atatcgaagt acagattctc ggggatacgc atggaaacgt catccatctt tttgaaagag
     7501 attgttctgt tcagcgccgt caccaaaaag tagtagaagt agcgccttgt gtctctatga
     7561 atgaacagca acgtcaaaaa atctgccagg cagctgtcca attgatgaaa tatgtcgggt
     7621 atgtcaacgc aggtacagta gaatttcttg ttgaaggaga tgatttttac tttatcgaag
     7681 tcaaccctcg cgtccaagtg gaacatacga tcacagaaat gattacagat attgatatcg
     7741 taacaacgca actgttgatt gctcaaggtt tggatctgca taaagaaatc ggattgcctc
     7801 agcaagaagg aattaaatta aatggttcag ccattcaatg ccgaatcact acagaagacc
     7861 cgttgaataa ctttttacca gacacaggga aaatcgatac gtatcgttca cctggtggtt
     7921 ttggcgtacg tctggatgtc ggaaatgctt acgcgggcta tgtagtgaca ccgtattttg
     7981 attcattgct agtaaaagta tgtacgcatg gtgctacctt tgaaacagcc attcagaaaa
     8041 tggaacgttg cttgaaagaa tttagaatcc gtggagtgaa aacaaacatt ccatttatgc
     8101 tcaatgtcat cacacatcct gaattccaat caggaaatgc aaaaacaacc ttcattgata
     8161 gtacagcaac attatttgaa tttcctcgac tacgagaccg cggaaacaaa acgatgaaat
     8221 atatcggtga aattactgtg aatggttttc caggtatcga aagtggcgaa aaaccatttt
     8281 atgaagaacc acgtatgcct aaagatctaa tcacaagaac tgattacgta acggctaaga
     8341 atgtattaga tgcgaagggt gccgatgctt tggtcgaatg gatcaaagga caggaaaatt
     8401 tgttgttgac ggatacgaca tttagagacg cccatcaaag tctgctagca actcgtgttc
     8461 ggacaaagga tttcaagcag attgcacgtt tgactggaga aggtcttcct gaactctttt
     8521 caagtgagat gtggggggga gcaacatttg atgtggctta ccgctttttg aatgaagatc
     8581 cttggcaacg gttgagaaaa attcgttctt taatgcctaa tacgttgctg caaatgcttt
     8641 tcagaggttc aaatgccgtt gggtattcta attatcctga caatgtattg gtggaatttg
     8701 tcaaagaagc agcagcgcaa ggaatcgatg tatttcgaat ctttgatagt ttgaactgga
     8761 ctccacaaat ggaaaaaagt atccaagctg tacgggatac tggtaaaatc gcagaggcag
     8821 ctatttgcta tactggcgat atcaacgatc caagtcgggc aaaatacaat gttcagtatt
     8881 ataaagacat ggcaaaggaa ctagaacaac tgggtgcgca tatcattgca atcaaagata
     8941 tggctgggct gcttaaaccg caggcagctt atcgtttgat tagtgaattg aaagaaacga
     9001 cagatctgcc aatccatctg catactcatg ataccagcgg gaatggaatc atcacttatt
     9061 ctgctgcttc aaaagcagga gtagacatcg ttgacgtggc aatgagtgca atgagtggga
     9121 atactagtca gcctagcatg agcagcttgt attatgcact ggtcaatggt ccacggctgc
     9181 cagaaatcac gattgaaaat gcacagaaat taaaccatta ttgggaagat gtgcgcatgt
     9241 attacaaacc gtttgaaaac ggcttgaatg ctcctgaaac agaagtatat atgcatgaaa
     9301 tgccaggagg acagtattcg aatctacagc aacaggcaaa agctgtcgga ttaggtcatc
     9361 gctgggatga aatcaaacaa atgtatcata ctgtcaactt gatgtttggc gatatcgtta
     9421 aagtaactcc atcatctaaa gtagttggag atatggcact atttatggtc caaaatgatc
     9481 ttacagaaga agatatttat gaaaaaggcg aaacactaag tttccctgaa tctgttgtga
     9541 ctttcttcca aggagaacta gggcaacccg ttggcggatt tcctaaaaaa ttgcaaaaga
     9601 ttattcttaa aggcagacct gcattgaatg aacgtccagg tcttttggct gaatcagtcg
     9661 acttcaacga agtaaaaaaa gaattggcag aaaaaattgg ctatgaacca aaacaagacg
     9721 aagtattaag ctatttgatg tatccgcaag tctttttgga ctatcaaaaa gcatataatc
     9781 aatttggcga tgttacatta ctggacacac caacattttt ccaaggtata cgtttgggtg
     9841 aaacaatcaa tgtacaaatc gaacgcggaa aaacgcttat cattcgttta gatgaaatcg
     9901 gagaacccga tatcgaaggg aatcgcgtac tgttcttcaa tttgaatgga caaagacgtg
     9961 aaatcactat caaagataat tcgatcatca gtacggtcca aacccgattg aaagctgaac
    10021 cgacaaaccg agaacaaatt ggagcaacga tgtctggttc tgttttggat gtattagtga
    10081 aaaaaggcga taacgttaaa aaaggtgata cgttgatgat tactgaagcg atgaaaatgg
    10141 aaacggccat cgaagcacgt tttgacggag aagtcgcaca tgtatatgtg tcttcaggag
    10201 acactatttc gtctggtgac cttttgattg aagtgactga gaaataataa gcgaaaggtg
    10261 gggaaacatg aaaagactgg gtttgttttg gctgattctg ctattagtta ttgtggcatt
    10321 ctacatggaa ccagtcttgt ttccgccaaa acttgaagtg aaggaaaact ctgatcaaca
    10381 aatatcaaac caaatgacaa cgtggaaata ccaggagctt aaagcaactg gtttttctac
    10441 ttatatcgga aaaccgatcg atcagctgga aaaggatttt ggccaagcct atgatcgatt
    10501 aactagcgga tttggttttg aaacaagata ttatgaaaat aaagctagtc atctcacatt
    10561 tgaagcgaac atccaagaca gcagagtcac aactgttaaa gtactgcaaa cagggcctgc
    10621 tgatatcgcg ccgttttatt tagggatgac gatgcaagat cttacgaaaa tcactactat
    10681 ctatactaat ttcacttttg agtaccatga tacagaagtt ggaatcgaac taatggaaga
    10741 ggatatgaat tatcgtccgc taattgcttt tgataacgac acttttgcta ttttattttt
    10801 tgatcaaaca acaaacggcc tattttcagt tgtgtattta gataaagata gtcttttgaa
    10861 actattgcca tatcaagtgt ttggtgaagg ccttccacat tatcaagcag ataaatcggc
    10921 tgattgggaa tcaatcaatc gcgttaaaga acgaaaaagt acgactttat taaatatgct
    10981 tagaagagaa gacgaacttc ccatatacaa tcaaacactt gcctttcaaa accacagtca
    11041 gctgctacta catgattttt tgacaaggcc tgaggaaata ttgtcagagg aacgtatggc
    11101 tgattggcaa aaatcgctag gcagtgctca aacgacagtg aaattctcac tgacgaatga
    11161 tgaattggag aagttaatca gtaaacaaaa attagaaaat actagtgggc tatttattca
    11221 tccggcgatt gacgctactt tttcgttttt atattggtac agcgatcctt acaaccatga
    11281 ccgtttcatg gacccgggaa cggattctgt aggcattgct ttttctaaag aaaatatgtt
    11341 agtcttattg caagaagaaa acggagaaag tagtgatagc agtgatcgtt aacgaagagc
    11401 tgtttcaact ggaggatcaa tgcctccaag tcgctgaaat gataaaaaag agtaaaacga
    11461 tggcttctta tcttgaacat aaacagtcaa tggaagaatc tgcaaaagtt tgccagttga
    11521 aagaagcgtt cttgtctaag aaagaagcat ttgaaaatgt agcggcatat ggtgagtatg
    11581 cacctgatta tcgtgaaaaa aaacgagctg ttcgaaaagc aaaaagacag cttgatatgg
    11641 atgagcatgt tgcagctttc cgatatagtg aaacacagct gcaaagtctg ttggatcaaa
    11701 ttgtccaagt acttgccgaa gaagtttcta atgaaatcaa agtagatgca ggtagcccgt
    11761 tttttgaaac gaaaaaacac tttggatgcg gaggtaattg tcatgcaatt agacaatagt
    11821 gaatttgaaa taacaaaacg acgcgggctg atcgtatggg tctacagctt aaaacaatta
    11881 aagaatctga aaaagttcgg cttgttgcat tatgtctcaa gaagaatgaa atatgtcgtg
    11941 ctttacttaa atgaagagac attggaagaa acagaagaac gaatccgtaa attacatttt
    12001 gttcgaaacg tagaacgttc ttaccgaccg gatatcgaaa tgaattttgc cgaaaaaata
    12061 ggaaacaaaa cagtattaaa agacgaaggt ttcgaaattg aagagctaaa tacagaaatc
    12121 cgattagctg aacatcttta aaaatctctt atgagcagtt tttaaataaa aaataacttg
    12181 gataacgatc gatttttgat cattgtccaa gtattttttt ggtatactag taccagtacc
    12241 atttctaagg agtgagtaga tgcggataat atcaggaaat tatggcgggc gccgtttaaa
    12301 aagtttagca ggggcgaaca ccagaccaac tacagataaa gtaaaagaat ccatattcaa
    12361 tatgattgga ccatattttg atggagagac agtattggat ctgtttgcag gaagcggtgg
    12421 tttagctatc gaagcagtat ctagaggctg cagtcatgcg gtatgcgtag acaaaaatta
    12481 ccaagcgcta aaaatcatca aagaaaacat cgaaattacg aaagaaccag agaaatttac
    12541 cacaataaaa cttgatgcgg acaaagcaat cgaacggcta gcagcggaga aacaacaatt
    12601 cgactacttg tttcttgatc cgccatatgc aaaacaaaaa ataatcgatc aaatccataa
    12661 aatggaagaa ctgggacttt tttctaaaac cgcagttatc gtatgcgaaa cggataaaac
    12721 agtcgaattg ccagaaactg tggctggatt ccatcaaata agaaaacaga tttatggcat
    12781 aacggccgta actatctata gaaaagaggg aaatcaatga aacgtgcatt attcccagga
    12841 agttttgatc cttttacaaa aggtcactta gatacggtgg aacgtgcggc taaattattc
    12901 gatgaggtag taattggtgt ttttatcaat acttcaaaga aaagtttgtt tcccccagaa
    12961 gaaagaatga cattgatcac aaaagctgtt tctcatcttc ctaatgtaaa agtcatgcat
    13021 caagaaaatc aattgacggt agaaacagca aaagagattg gtgcagatgc gttgatcaga
    13081 ggcatacgaa gtatcaaaga ctttgaatat gaacgagaga ttgctcagat gaatcatcat
    13141 ctatatgatg aattggaaac tgtcttctta ttagcaaaac cagaatatag tcacgtgagt
    13201 tccagtattt taaaagaagt actgcacttt ggcggagatg tgagcagcta cttaccgcca
    13261 gtgatcaacg aagctttagc tggaaagcgt gaatcaaatg aaagctaaac aatggttgaa
    13321 aacagtactg attatcatat ttggaatgtt gatcattgga tttgtagtag ttcccttgcc
    13381 gtattatgta gaatcaccgg gagccacgat cgatctgaaa gaactaatta cggtcaatga
    13441 taaggaagat aaaggttctg ggtctttctc tcttacctct gttggtatca ggcaagctac
    13501 aggtttctta gctttaaaag ctaaaatgac cccttttgaa gaattgatca gtgaagaaga
    13561 attgactggt ggggcgacaa atgaagaata taatcaaatg caacagtatt atatggattc
    13621 ttcccaaaat gcagcaatcg aacaagcgtt aaaacttgca gacaagcctt ataaaatgga
    13681 attcaaaggc gtatatgtca tgagtgtaga gaaaacatcg aatttctttg gcaagctttc
    13741 agttggagat acagtaacaa aagtagacgg gaaaagcttt caaagtacag aagcatttat
    13801 ggattatgta aaaagccaaa aagttggaca aacaatcgaa atcagttatt tgcataatgg
    13861 tgaagaaaaa acagcttctg gtgatctgat cgagttacca acagataaaa aggcaggaat
    13921 aggtatcacg cttactgacc ataccgagat cgaatcagat acagatgttc gtattaattc
    13981 tggctctatc ggtgggccat cagcgggatt gatgttcact ttagaaatct atgaacaagt
    14041 cactggtaaa aacttgcgac atggtaagca aattgcagga actggaacga tcaacagtga
    14101 aggagaagtt ggccgaatcg gaggaatcga taaaaaagtg gcaagtgcgg ataaagctgg
    14161 cgtagagatt ttctttgcac cagatgatac gatttcagac gatgttaaga aagaatatcc
    14221 tgaaataaaa accaactacg aagaagcaaa agctgccgct aaaaaattga attcctcaat
    14281 gaaaatcgta ccagtaaaaa cagtacaaga agcgcttgac tatctagaga aaatgaaatg
    14341 aaactaaata attaaagagg ggttgtgaca cgagttctgt cacaacccct ctttttcgtc
    14401 aaaaatcttc acgataaaaa taaaaacaaa agaaaaaatt gacgtaatta gatgttgaag
    14461 ggacgatttt gccagagctc actgtttctt caattttaaa tgaaaggagt aatgaatgat
    14521 ttttactggt cattctttga aaaaagaaca atgatagaaa aaatcagcaa gaaaatacta
    14581 gcttcaccca aattgatcat ttggatgtct atgatagttc ttatcttttg tatcgcaagt
    14641 ggttttttat tatcaaattt gctattttat agagaaacag aagagactgc tgcaaaagaa
    14701 atactttctt cgtttagttc cagtggagaa gatccatcag ctgaaggaaa cgatgtagaa
    14761 gaggatgctc gtccaaaggt catgtacacc gatatcaaag gaagtgtgaa ggagcctggt
    14821 atctattcct tttcttcaga agaacgggtc tatgatgtac taaagcgtgc aggtggatta
    14881 ttagaagaag cggatagcga tcgaatcaac ttttctgcga agatagaaga tcagcaagtc
    14941 ctatatatcc ctgctgtagg tgaagagctg ccagaacatc ttgatcagtt ggcatcctcg
    15001 gaaggaaaac agcctacagc agattccgag cctcccaaaa taaatatcaa tacagccact
    15061 ccatcagaac ttcagcagat tcctgggatc ggatcagtca aagcgcaaga aatcattcga
    15121 tttagagaag aaaacggatc cttccagaaa gtggaagacc tgcaggagat ttctggtatc
    15181 ggtgaaaaga cagttgaaaa actaaaaaac ttcgttacaa ttaaatagaa tgattcaaaa
    15241 tcgactggca cgagcaataa atggacgcta atggatacta agaataatcc aattctaaga
    15301 gcttggcagg acgaggaggt agtatttttg gataggaaat agcttgattg ttcgcaaaga
    15361 aacattaaaa aaaccaatgc tgaatgtcta tctccaaaat aaaatatctg ggattcatat
    15421 tatgaatacg gctgtcagcg gcaataacag tcaagctcta cgagaacgct ttgccaaaga
    15481 tgtacttagc tatacagcag ataaagtatt tattctcatt gggacgaatg atctagcaga
    15541 gcataaacaa ctttctaaag aaacgtacca aaaaatctgc agtggatgat agttcagctg
    15601 aaaaaagact atagttctga tcatgctttc tttatcacgc caccagcagt cgatgagaca
    15661 aaacaaaaat atcggaataa caagttgatc aaagaatata tctctgttct cacagaagta
    15721 gcaaaacgcc atgattgtga agtatcgatt tttatacgga atttcttgaa caaatggatt
    15781 cgaagaattt ttataatggg ctaatggatg acggacttca tttcggcagt gaagggtagg
    15841 atcttttgtc agacaggatg attcgttata tttaaagaat attttgttta agcaatgagt
    15901 actttttaat caacttgaaa aaacggagga atataaaaca tgttagaaaa atcttcaaaa
    15961 gcatgcgata cgaataaaga aaaagttact gataagtcac ataatgaacg cattccttgg
    16021 gaccagtact ttatggctca ggcagtactg ttatctttac gtagtacgtg tacacgttta
    16081 gaagtaggag cgacactcgt tcgagaaaaa aggatcattg ctggcggcta taatggagca
    16141 gtcagtggag atgtccactg catagatgaa gggtgttacg tcgtggatgg acactgcttg
    16201 cgaacgatcc atgcagagat gaatgcactc ctacagtgtg caaaacttgg tatcccgacg
    16261 gaaggttcag aaatctatgt cacacatttt ccatgtttgg cctgtacaaa ggctttgttg
    16321 caagctggtg tgaaagggat caattatctc catgactatc gaaatgatcc ttatgcccaa
    16381 gcgttgatcg aacaattagg tgtatctgta caccaagttg ctttagactc acagcatttt
    16441 tcaaaacttc aaaatgaatt aacaggaaaa catgtctagc gttttagaaa ggcatgttcg
    16501 aaatcggttg atttttccgg caattacgtc gatgattatc tgtttgttgt attacactga
    16561 caattggggt tatcgtttgc ttcttgccgg aattttttta tggttttcgc taaaagaagg
    16621 acgttttatc gcgtacttgt gtattttgtg tagcataacg gtatccattt cttataggct
    16681 acaagagtat tcatttttag aggaaaaaac aacatctgcg caaattactg tatttacaga
    16741 tacgattcgt gcaaatggta attttttgac aatggaagga aggttatcag agaataaaca
    16801 aaagctccag ctttcttatc aagtaaacac agaagcagaa ttgaaaacat ggcttaactt
    16861 ccgaggaaag ggaatagaat tgactgctga aggcgagttc atctctccag caaaaaagcg
    16921 gaatctttat aattttgacc aagaagctta cttcaggaac catagaattt ctggacggtt
    16981 ccaaattcaa aagattgaac agatcaccat gcaaaatcac tggcgaaact ggcttcagag
    17041 aaaaagagga tcttttatct cctttgtcca atctgaattt ccgcaaaaaa caagtgtata
    17101 tattaattct ttattatggg gctataaaga tgcaggattc agagaaccag aagaaatttt
    17161 tagggagagt ggtttgctgc atctgttcag tctatcgggt ctacacatcc aattttattt
    17221 agggtggctg tattatttat ttcgacgtat cggatggcca ctacacgtca gtattattcc
    17281 gttgagtcta ctaactatct gttatgtttc tattgcagga agttctattt ctgttttgcg
    17341 tgctagtttg ctgtttttat tgcgattgct agtcaaattg ctgaataagc attattccac
    17401 catggactta tttgcagttg tcttttggct tcttttacta tttgatcctc gatttctttt
    17461 tcatgcgggt ggacagctta gttttttcat gagttttttg tttatcctga tttcttttga
    17521 tggagattgg ttgaaaaaaa cggcctctca cgtcctgttt actatgatca cgatgccgtt
    17581 gattgtatgg catttttatg agtggcctat tttaggtagt ctttttacat tactgattgc
    17641 accggtattc aagtttcttt ttcttcctgg tgtgttattt ctcacactct tcaatcgctg
    17701 gctgccggaa actttacttt tgctgataga agagggatta atcttatttg agcgaataat
    17761 cgatttttct tcagggtcgt ctctcattat aggacgtttg ccccttgtgc tatctgggat
    17821 gtatattttc ggattactga tttgtatgga taggttgaaa gaacaacctg tgaaacactt
    17881 gttttatgtt ggtctttttt cttttatttt tttgactttg ccatttttac gcttttcttc
    17941 aacgatcgct tttatcgatg tcggtcaagg agacagtatc tttttgcaaa gtccttttcg
    18001 aaaagaaacg attttgatcg atactggagg tcagttacac tttgaaagag aaaagtgggc
    18061 gcaaggtatg atacgtcctc ctgctgaaag aaatatcgtt ccatatctaa aaggtcaggg
    18121 gataagtgta atcgataaac tcatcttgac acacgacgat acggatcatg tgggagaatt
    18181 acccacgtta tctcagcatt ttactatcaa aaaaatttat atcggttggg gcgctggacg
    18241 aaatgagcca ttacaaaggc atttatctga agctcaaaaa aatggtactg agatcattga
    18301 aatcaaacat ggggacagga tcagcggtta ttatgatctt tatgtattga ctccttttga
    18361 aaagggagag ggaagaaatg aagattcgat tggtctatgg atggaatata acaataggcg
    18421 ctttttattt ttaggagatt tgaatcaagc aatggaaaaa caattgcttt tgatttatcc
    18481 taatctgaaa gccgatgtag tcaaattagg tcatcacggt agccgtacgt caagtaacac
    18541 agattttctt tcccatattg aagcaaaaca tgggattata tcctgcggcg taaataatcg
    18601 ttatggtcac ccgcacagcg aagtattgga gacactagaa gaaaatcaga taaaaacatg
    18661 gcgaacggat caacaaggaa tgatcaccta tcgctggcat ccagtttttc atcctcatgg
    18721 gctagtggaa acaatgatag actagtaatt ctcatctgct catggtagga taatttttga
    18781 aagtggtgag taatttgagt atacaagcag agttacaaaa aatacgtaaa gaaaaacttg
    18841 catcttgtta cttgatttta ggaacagaaa agtttctcca agatcaagtt cggacagaaa
    18901 ttgtcaaaaa aatccaatta gagggcgaag atgacttaaa ttttttaagt tttgacatgg
    18961 aaaatgcaag tttagatgag gtagtagctg aagcagagac gttgccgttt ttcggagatc
    19021 aacggttagt attcgtagag aatccttatt ttttaacagg tgaaaaagta aacaatggaa
    19081 tcgaacaaaa tactgattta ttgacggctt atttgaaaga accattagaa tcgactgtat
    19141 tagtattctt tgcgccatat gaaaagttgg atgaacggaa aaaagtaacg aaacaattga
    19201 aaaaaacagc aattatcatc aatgtccaac agttgaatga aaaagaagta cgtcaatatc
    19261 tgatgaatac cttagagaat tcctcaatca aaatggaacg aagtgcaatt gatttattct
    19321 tgaggttgac cgacttagat ttatccaaaa tgatgagaga gttacagaaa ttggtccttt
    19381 atggacaaaa tcaacagcaa atcacggtaa gagaagtaga agaattagtt cctaaaacgt
    19441 tagaacataa tatttttgac atgacccaat atattttaaa agggaagacg gaacaagctt
    19501 tgcgtttata cgaagattta gttatgcaag gggaagaaac aatcaaaatt aatgcgattt
    19561 tgctttctca attacgttta tttttacaga caaaatttct tgtaaagatt ggttatcagc
    19621 aggcaaatat tgctgaaaca ttgaaaatcc atccatatcg tgtaaaactt gccatgcagg
    19681 aagtaaaaaa attcgatgaa catttgctgg tacagttgtt tgatcaatta gttgagatgg
    19741 attatcaaat caaaacggga caaatcgaaa aagaattaag ttttcaacta ttcgttttgc
    19801 gcacagggca gttgttaaga aatcgataag aagaaagacg agggcaaagc atgcgggaag
    19861 gtcgtttgtt tttgtccttt ttttgacaaa taaaaaaatc ggccgaagcc gattctttta
    19921 tttagcgatt tttttgctta aacgagattt gtcgcggtta gctttgtttt tgtggattag
    19981 accttttgat tcagccatat caatagcttt aacagcttct ttatataacg catctacgtt
    20041 gtcagcacct gaagctacag catcttcaaa ttttttgatt gcagtacgca tagcgcttgt
    20101 ttgagatgaa tttttcacat tagcgttttc gcttgtacga acacgtttga ttgcagattc
    20161 aatgtttggc attagtttca cctccgataa cttaagttct tacgcgtgtt accgcataat
    20221 tcaacattca ttattatacc taaaggcagc gtgctttgca atatgaacat gacaagtttt
    20281 tttccttaac agtcttttat taggattttt tttcagtaaa aacggatagt acaagctgaa
    20341 aaaaatcaaa aagacgctat tgtgggaggc aatagcgtca aaccaaacta gatttttatt
    20401 tatctattta aacaaagttt gaatgggaat gtttggtact aagttattat ataacaaaga
    20461 gaaccgtttg caaagttttt tttgaaaagg gatacgattc cctgttgttc tttgtttttc
    20521 tattaagaaa ctgcagttat tttataacca gtatcctttg atcagtaaag gctattctgg
    20581 tgtaccgatt attttaatgt attcatcgat attttttatc aacatttttt ttatgaccaa
    20641 ctggttccga cggttccatt tgccacttac ttccagcaca tatttattta ggggcaaacg
    20701 taagatatat tttgccaaat agcgatcttt aagcaggcca ttgatcacac ggtcctcaag
    20761 ctgcaagttg atcccagtca gcatcattgg ctgttcttgt aaaacgtacg tatcaattaa
    20821 aagaccttca aaagtttcca caagcgtctt cctttctttt caaagtaacc tgaaaacagg
    20881 cactaattct tccattttga tattcgttga agtggatgcg atcattcttt cttttgtcca
    20941 ctgtaatcta gtataacaaa aaagagcaga agagccaaaa agaaaaaaga gcatcgaatt
    21001 ctataggaag tctcattggc acggcctaaa ttattacaaa acattgatta aaaaaagccc
    21061 ctgcatcgca ggagcatccc ctatatcgtt ttaataggtc aatcaaaaag cagtatcaaa
    21121 cacattttgt atcaaatcag gaaataatag ataagctaag cccagaatca aaagtaccgc
    21181 tagaagaaac cagccgattg ttcctatgaa atactttaat ttttcgaaca ttgtatcacc
    21241 tcgttgtcag tatatcacgg atcagtaaat tgggggtgtt aagaatctcc taaaatagac
    21301 aaaatttgtt ctattttcct aaagcaaata cttgtaagat acagtaagtg ataccagaag
    21361 caatgaccgg tccggcggcg attcctctta agaagacaac acctaaaatc gtgccgaaaa
    21421 caagtgcaac agtgatttgt ggatcagaag caatcaatcc tacacccttg gaagataaga
    21481 cagccactag cccaccgcaa aaaacagcta cccagccaat aggggattta aaggcttcta
    21541 atagatcacg tagaccgatt tctcctgttg cgatagggat tagaatagcc gctgtgatga
    21601 tcgtcacccc aatattgatt cctttgcctt gaagccaagg gaaaaaggaa cgggaggctg
    21661 gtatgagctt cataatcaaa acaaaagctg ccgctatcat cagactttga tttttagcta
    21721 aaattgctac aagtaaaata attcctaaaa aaagccaact ttccatgaaa tatattcctc
    21781 actttcttcg aaacaatcat aacatgtttt tagaaagtta caaaagtagg aattcatc
//
LOCUS       DAZUMK010000038        21573 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000038 DAZUMK010000000
VERSION     DAZUMK010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.038"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(308..2689)
                     /locus_tag="ACG808_001369"
     CDS             complement(308..2689)
                     /locus_tag="ACG808_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFS5047002.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2679..3326)
                     /gene="recU"
                     /locus_tag="ACG808_001370"
     CDS             complement(2679..3326)
                     /gene="recU"
                     /locus_tag="ACG808_001370"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFS5047003.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3390..3929
                     /locus_tag="ACG808_001371"
     CDS             3390..3929
                     /locus_tag="ACG808_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFS5047004.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4019..4438
                     /gene="gpsB"
                     /locus_tag="ACG808_001372"
     CDS             4019..4438
                     /gene="gpsB"
                     /locus_tag="ACG808_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFS5047005.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4460..4827
                     /gene="rnpB"
                     /locus_tag="ACG808_001373"
     ncRNA           4460..4827
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACG808_001373"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5036..6202
                     /locus_tag="ACG808_001374"
     CDS             5036..6202
                     /locus_tag="ACG808_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFS5047006.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6248..7744
                     /locus_tag="ACG808_001375"
     CDS             6248..7744
                     /locus_tag="ACG808_001375"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFS5047007.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     LLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7825..8508
                     /locus_tag="ACG808_001376"
     CDS             7825..8508
                     /locus_tag="ACG808_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS5047008.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9153..10343
                     /locus_tag="ACG808_001377"
     CDS             9153..10343
                     /locus_tag="ACG808_001377"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFS5047009.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10445..11398
                     /locus_tag="ACG808_001378"
     CDS             10445..11398
                     /locus_tag="ACG808_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFS5047010.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11467..12816
                     /locus_tag="ACG808_001379"
     CDS             11467..12816
                     /locus_tag="ACG808_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS5047011.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(12999..13322)
                     /locus_tag="ACG808_001380"
     CDS             complement(12999..13322)
                     /locus_tag="ACG808_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047012.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13451..14614
                     /locus_tag="ACG808_001381"
     CDS             13451..14614
                     /locus_tag="ACG808_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFS5047013.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14684..16033
                     /locus_tag="ACG808_001382"
     CDS             14684..16033
                     /locus_tag="ACG808_001382"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS5047014.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(16078..16508)
                     /locus_tag="ACG808_001383"
                     /pseudo
     CDS             complement(16078..16508)
                     /locus_tag="ACG808_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            16896..19538
                     /gene="alaS"
                     /locus_tag="ACG808_001384"
     CDS             16896..19538
                     /gene="alaS"
                     /locus_tag="ACG808_001384"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFS5047015.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19738..20334
                     /locus_tag="ACG808_001385"
     CDS             19738..20334
                     /locus_tag="ACG808_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS5047016.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20456..>21573
                     /locus_tag="ACG808_001386"
     CDS             20456..>21573
                     /locus_tag="ACG808_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFS5047017.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLE"
BASE COUNT         6980 a         3795 c         4556 g         6242 t
ORIGIN      
        1 ttcaaaacca tatattgtat tttttatttg ataataacaa tgggattatt aagaagtggc
       61 actaaataaa agactagtgt gtatttagcc gatttaatta taaacaagta aatggataaa
      121 gtatatgtaa tgataaaaga aaaatggatt ttctaatttt taagctaaat gttagatcac
      181 acatgtatat gctggatagt cttataaaaa atcatgtaat acagcttggt acatgatttt
      241 tatttgtagc tgttttttac atagacacaa aaaagaaacc acgcttgtag acgtggtttc
      301 ttttttacta tctgtttcca gaagctgagg tgctgctgga tggtgtatgg tcatttgcag
      361 catccgctcc gctagttgct ggctcactgg aagaagcagg aggagcagat gaagacggtg
      421 tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga agacgctggc tcgcttgttt
      481 ctgctgtcgt gctttctttt gaggactcag cagttgattc gctttgacta gtagagctag
      541 agctttgagt cgttgtctct gttgttgagc ttccaggagt ctggacatag ctggaagatg
      601 gaatagttgt tgatggtgta attgcattcg atctagctgt atattgatct ttgtagtaca
      661 gttcaccgcc cacacgaatt aatccgcttg gcatctccca gtctgtattt gtcacgttag
      721 ctgaaacata ctgcataagc tcgcgataaa catcagaggc tacgtgtgac gattctgaag
      781 tgacaggtgt cattttttta ttgtaacctg tccacacgga aatcgaataa tttggcgtat
      841 agcccgcaaa taggatatct ggatacaccc cagaagaaat accaagtttt gcatactcgt
      901 catctgtgta attggatgtt ccggtcttac cggcttgata taacccagcg atttgcgcat
      961 tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc tgtgatcata taagctgttt
     1021 ctggagacat ggctgttttt ccatctggct cataagtttc ttctgttccg tcttggaaga
     1081 cgattttatt aacatattga ggtttgtaat aagtccctcc attggcgaaa gccgcatagg
     1141 ctgctgccat ttttaaggaa gaagctccgt attttgtccc atcttgttct tccgtattac
     1201 tagagatcgc attcgattga tggatcgtac tgtattcgat cccaagattt tttaagaaag
     1261 aagccacttt atctgaaccg acttcattga ataatttgac tgctggaaca ttacgggata
     1321 agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg attatcccag tttcctacag
     1381 gagtcgaagt cccttcatag ttatacggtg cgtcagtaat agtttttcca gtagaatatt
     1441 tcaaatattc aaaagctggt ccgtaatctg tgacaggttt catcgtagaa ccaaaatcac
     1501 gggaagtatt cactgctaag ttgttgccta aagttacgtc ttctgcgatg tgacgtccgc
     1561 caatctgggc ttttactttc ccggtattgg tgtcgataag ggtggaggca acttgcatct
     1621 cgtcgtctgg gtaagagaca tactgatccg tgttgacgat atcatatagt ttcttttggg
     1681 cgtccagatc caaattcgta taaatctcta aaccgtctgt gtagacattc ttatctgttt
     1741 tttcttgtac ttcattgatg acttctttga cataattatc gacgatctta gtattgtcat
     1801 cggattgagt tagttcttgc agaccatccg ttaccggtac attaacagct tgatcatatt
     1861 ctgtttgaga gattttttcg ttttgaagca tcgtatacaa aacagtatct cgtcttttct
     1921 tcgcctgatc tggataaaca taaggatcat aggcggaagg ggcttgaggc atcccagcaa
     1981 gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt accaaagtac atctcagaag
     2041 ctgtctccat cccgtacaag ccgtttgaca tatacacttt atttacgtag taggtcaaga
     2101 tctcttgttt cgattttttt tgttccaatc ttacagccat ccacgcttct tgggccttgc
     2161 gtttcagagt ctgatcctcg gcactggtgg agaagaagga aagtttgatc aattgctgtg
     2221 tcaaagtact tccgccttgc agtccgccag aggtaaagtt agagagggca gaaccgatga
     2281 tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg atcttctacc gacacgatcg
     2341 catcttctaa tgttttcggt aattcattcg cagaaatttt ttcacgcttt tctgcgccta
     2401 aatcttcgaa tagttcgcca tcttgtgtat atagtttgga agatacggta gcgtctaatt
     2461 ttttatccgt caattcgggc gcatcttttg cgtaatacca gaaaagtcct acacctgcta
     2521 aaaagaggat acagaaaaat gagagtattc ctaaaaggat cttgataaat aatccttttt
     2581 tatgtgttcc agaggacttt ccagaacctt tgccagaatt cttcttcaca ctctttttag
     2641 gagtaggctg tttctgtcgt ctagagcttc tagtttgttc atttgccatt cttgctttct
     2701 ccttcaatag ataatgttga taaatagaag tcaaccgcgt ccaaataagg aatccgcgga
     2761 gaaataccag ttttgatctc tattcctagt tcttcgatca aagataaagg tagtgatttg
     2821 cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat gaacactatt caaataaaaa
     2881 cagcgcttca aagacgaaaa ccataacaag acaaagcaga ccccattttg ttcgaggcat
     2941 gctttcatat gttcgatttg atgggcatgg aagtttttaa agggaaaaga tgttttgttc
     3001 ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat agacgccatt atagtctgta
     3061 gttgaggctt gggtaaagta ggcttccttg atcacagcag cacttctctt aggatagtct
     3121 actttcacga tttggatagg ggtaggcttt ttatggataa ccgccaaccc gcgggaaaga
     3181 taatatttat tactttcatt gattgcttct tcaaagcgca tcccccgatt gccgaattca
     3241 gtgatctgat ttcttttttc ttgaattttt tcgacgggag aggcattctt ataataaggt
     3301 tggccatttg gatagcgcaa taccatcttt aatcacactc ctaggccaca ttatagcaaa
     3361 aaagcggata gaaagaaaag gaatctgaga tggataagtc aaaagtaatc tatgtgtctg
     3421 ggtatcgaag tttcgaactc ggtgttttta aagaaaatga tcctaaaatc caagttgtta
     3481 aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga agggctggaa tgggtacttg
     3541 tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat agtagcggaa ttgaaagaag
     3601 aatatccgga actatctctt ggtattttgt atcctttttc tgattttggt aataactgga
     3661 atgaagggaa ccaagaaaaa aaacaattag cagaacaatt agcagattat gtcgaagctg
     3721 ttagccatca accctaccaa tcacctagtc agctaaagaa tcacactcgt tttatacttg
     3781 agcatacagt gggctgtctg ctgatctatg atgaagaatt ccctggaaaa acgaagtatt
     3841 tttttgaaga tgcacagaat taccaagcag atcatcccta cgaaattcgg ctgatcagta
     3901 tggacgatct tcaaaatttt agtgaataat cgatattgtg tttgatttat caactttttt
     3961 cctatacaat gaaaaaggta gaaatgttat tacgtaaatg aaaactagag gtgtaaatat
     4021 ggcgaatttg gtttatagtc ctaaagacat tttacaacaa gaattcaaaa caaaaatgcg
     4081 cggctatgat cccgttgaag ttgatgaatt cttagacaat atcatcaaag attatgaaac
     4141 ttacagcaaa gaactccttg ctttacaaga agaaaacgat cgattgagtg caaaagtagc
     4201 tcaattatct aaaactcaag gagcagctca aacacgcgtg caacaaacag aggcaccaaa
     4261 gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg tcaaatcttg aacgtgaagt
     4321 atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa ccagcacaac ctaatccaaa
     4381 taactacaca aatgcagaca caagcttaga tgataatgaa aaaacacgtc aattttaatt
     4441 tttgattaat gaatttgcgg ttttcgggta atcgcggtct gacttgatca ggctgaggaa
     4501 agtccatgct cgcacaggct gtgatgcctg tagtgttcgt gcttagcgaa accataagct
     4561 aaggtactct ttggagtaac ggcaggaaaa caagctaagg cttcggctat gcttaagtat
     4621 ccttgaaagt gccacagtga cgaagcggct ggggaaaccc atccggtgga acgcggtaaa
     4681 cccctcgagc gagcaaccca aacaatggta ggggcgcttt ttgtctggaa gaagaacagg
     4741 acaaaaaggc agtataactg cagatagatg gttaccgctt tgttttttct ccctggaaaa
     4801 gcaaagtaca gaacatggct tatagaaaac cgcaaacatc agggaattag gtcgtgacaa
     4861 aagtaatgcg ttataagaaa caagaaaaac atttgaaagc tatttttcaa tacttcagat
     4921 gaatcgttct tattacaaaa caaacttttg tatcaccggt tattcggaat ttgagtaaaa
     4981 gtgtggggta ggactgatgt cccacctcct ttttttataa ataaggagat tgtgtatgga
     5041 aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc gggctagaag cactggttgg
     5101 taaagaatta agagatcttg gaatcgaatg ccaagttgag aacggacgtg cacgttttga
     5161 aggaacaatg gaaacgatcg ctacagctaa tttatggtta cgtacagcag atcgagtaaa
     5221 aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa ttgtttgaaa acgtaaaagc
     5281 attgccttgg gaagattttc ttccactaga tgcagctttt cctgtagctg gaaaatcgat
     5341 caagtcgaaa ctatatagca cacctgattg ccaagcgatc acaaaaaaag caatcgttga
     5401 acgtctccgt acctattatc accgtccagc ttctgtacca ctgacggaaa ctggcgcaca
     5461 ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg gtaactctgg atacgacagg
     5521 accaagttta ttcaaacgtg gttatcgctt agaaaaaggt ggagcaccat tgaaagaaaa
     5581 catggcagca gcacttatta tgttgacaaa ttggcgaaaa gaccgtccat tttatgatcc
     5641 tgtatgtggt tcaggtacgc tttgtattga agcagcctta ataggccata atatcgcacc
     5701 aggattcaat cgtgagtttg cttgcgaatc ttgggattgg ttcagtcaag aaatcatgga
     5761 aaatgttcga tcagcagccg aagaaaaagc agattatgat attgaattag atattactgg
     5821 ttcagatatc aatggacgga tgattgaaat tgccaaagcc aatgcagaag aaatcggatt
     5881 aggctcttcg attaccttta agcaacaagc agtcaaagac ttcaaaacgg ataaagaata
     5941 cggggtcatc gtggctaatc cgccttacgg agaacgtcta ggagaagaag aaactgttcg
     6001 gaaattatat aaagaaatgg gcgatgtatt ccgtccatta aaaacatgga gtaaatatat
     6061 cttgactagc gacttagcgt ttgaggaatt ttacggacaa aaagcaacca aaaaaagaaa
     6121 attgtacaat ggtgcattgc gtacggatct attccagtat tggggaactc gtccgcctag
     6181 aaaaaaagag aaagacgaat aatcttacta attgattcat tcaattggat gtttggagga
     6241 atagggaatg aaagaaaaag agtttttaca agaagtaaaa gaaatcaatc ttctacaaca
     6301 agctttagga atcctcgatt gggatacaca aacaggtatg cctgaaaaag ctagtgcata
     6361 ccgaagtgaa gtagacagct atctttacag catttatttt tcaaaaaaaa tcggtccaaa
     6421 aatcaaagaa gccattcgct atttttctga acatccggaa gaattgtcgg aagttggaac
     6481 tgccgttttt gccaaagtaa aagaagagta tgatttggaa catcgcgtac cggaagatct
     6541 tatgagaaaa tatacagctg ccgtttcttc agcacacgga caatggcaaa aagctagaga
     6601 aacacaacaa ttttctgatt ttcaacacgc attgaatcaa aatattgaat taacaaaaca
     6661 gttgatacct tactggagaa aagatgaaaa gacgaattat gatgttttgc tgaatcaata
     6721 cgaacccggg atgactgtcg aaatccttga taaggtattt gatcagctta aagaaggaat
     6781 tctttcgatc cgccgtacgc tccaagaaaa agggactgaa cctgaaacag actttttaaa
     6841 tcggagaatg acgaaaaaac aacagaagag atttgtagtg aaagtaatcg aacaacttgg
     6901 ttatgatttt agtcgtggtc gtttagatga cactgtccac ccatttatga ctggaattaa
     6961 tccgaatgat gcacgaatca cgacacgatg gaacgaaaca gatttcaaaa tggctgtttt
     7021 tggagtgatc cacgaggctg gtcatggtat gtatgaacaa gacatcgatg ataaatatgc
     7081 gtatacaccg gtttatgaag gtacatcaat gggtatccat gaatcacaat cgttgtttaa
     7141 cgagatcatt attggaagta gtcgcagctt ctggaaaaag caatatcctt tctttcaaga
     7201 atgtgcagaa ggaacattcg atgatattcc ttttgacaaa ttttatcgtt tattgaacca
     7261 tacaaaatca agtttggttc gtatcgaggc agacagtttg acttaccctc tccatatcat
     7321 cattcgttat gaaatcgaaa aaatgatttt caatgaaggg gtagatgttg ctgaactacc
     7381 tgaaatatgg aatcaaaaat atgaagagta tctgggaata cgaccaacaa atgatgtgga
     7441 aggcatccta caagacgttc attggtcagg tgcaagcttt ggttattttc cctcttatgc
     7501 attagggtac atgtatgcgg cacaattact tcattctttg cagcaagaaa tagatgtgga
     7561 aaatgttcta gcgtcagacg attattctcc aatcaaaaac tggatgtcag agcatatcca
     7621 tcaatatggt gcatctagaa aaccaaatca attaatcatg gatgcaacac atgaaccgct
     7681 taatccgcaa tacctgatcg actatatgaa agacctgtat tttaatgtat atcaggtaaa
     7741 ataaaataca atttacaaag atagctgaaa ttcatctaat ctatagatgt tttcagctat
     7801 ttacttaaga aagaagtgga acgaatggat aaattacaag tgatcgctga ttatagtatg
     7861 gaccaattag cgcatgatca aacaggacat gggagcgatc ataccaaaag agttgtcaag
     7921 ttagctgaaa aaattttgga gacggaaccg caggcagacc gctttgtgac gcttgcagca
     7981 gcttacctac atgatacgat tgatgataaa gtcgttaaaa atgagaacga ggcaaagcaa
     8041 cagcttcgtg tattccttcg ctctttgcca atagtagagg aacagatctc gatgattttt
     8101 tccattatcg aaaatatgtc cttttctaaa aatctctctg aagcggccga actatctctg
     8161 gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat taggagcaat cggcattttg
     8221 cgtactgctt atttcggcgg tggacatggg cacccgattt ttgatcctga actgtatccg
     8281 caaacattca aggacaaaga aaattaccga aaaggcacga cagtgatcaa tcatttttat
     8341 gaaaaactgt tcttgctgtc tgataaaatg aatactgatt acggatatga agaagccaaa
     8401 cgaagagaaa ccttcatgcg tgaattctta gaagaatttt ttctggaatg gtctattgac
     8461 gatgaaaatt ttactccaga caattggatc aaaaataaca aaaaatgatg gaggttgtga
     8521 cagaagtggt cagctccaag atatttcgga tcattttccg taaaagttac ttctggaaca
     8581 ctgtttacca agaggtcgtg agcctgccgt tcagctccga gtcacaagga acaatttcaa
     8641 aaaatagctt ctcgtgtttg atgaatattg taacttgtga ttcggaattg gcaggagaac
     8701 accgtttatt cggaagaata ggactgtgac aagacttttg tcacagtcct tttttcgaat
     8761 tcaacaagta aaacttttaa gaaatattca gagagtaaaa ttaaaaattg ctgataaacg
     8821 aagatggaaa ttttatgtat ttttattggt ctatgcaata aataaattaa atattcagaa
     8881 tacaattgat tttaacgaaa gggcttctat caatggttgt gttgtattta tctgcccagg
     8941 tagagtaaaa tagatcatta tctaaaaggt aattgttcat actatataag tcatttgaaa
     9001 gtaaaaagat actgtaaata aaaaatgtgg ttaaataatt ctcataatca gtgtaagcgt
     9061 tatcaaaaat atagcatact tgctttgtag atgaatgaca cgatatatgt ttggtatgtt
     9121 ctgcaagaca gatgaatatg gaggtggaga caatgtcttc caaagaacta acaatgtatt
     9181 tagagaaaga attggatcaa ctgaaagaaa aggggttata caatacgatc gacatactag
     9241 aaagtgaaaa tggtgcttgt atcatcgtag acggaaaaaa gatgatcaat ctagcatcca
     9301 acaattatct tggatttgcc aatcgcgaag aattgaaaaa agcatgtatt gaagcaaccg
     9361 aaacttatgg tgtaggagca ggagcagtcc gaacgatcaa tggttcgctg aaaatccatc
     9421 agcaattaga agaaaaaatc gcagaattca aagggacaga agcagcaatc gcctttcaat
     9481 ctggattcaa ctgcaatatg ggcgctatct ctgcagtaat gacaaaagaa gatgctatct
     9541 tatcggatga attaaaccat gcatctatta ttgacggttg ccgcttatcc ggagcaaaaa
     9601 tcatccgaat taaacatcaa gatatgaaag accttgagaa aaaagcaaag gaagcgatag
     9661 aaagtaagaa atataaaaaa atcatgtata tcacagatgg tgttttctcc atggatggag
     9721 acattgcacg attgccggaa attattccga ttgttgaaaa atatggactg atcacctacg
     9781 tcgatgatgc gcacggctca ggcgtaactg gaaaaggtgc aggaaccgtc aagcattttg
     9841 gattaagcga caaaatcgat atgcaaatgg gcacgttatc gaaagctata ggtgttgttg
     9901 ggggttatgt agctggatcg aaaacactaa tcgactggtt gaaagctaga agtcggccat
     9961 tcctattttc tacttcgcta actccaggtg cagctgcagc tgcattggct tcgattactt
    10021 tgatgcaaga acatccggaa cttgtcgaaa aagtttggga aaatgctaat tattttaaag
    10081 aagaattgaa aaaagtaggc tataacatcg gtatgtctga aacacctatt actccagtca
    10141 ttttgggaga cgaaaaagtg actcagactt tctctaagaa gctgatcgaa catgggattt
    10201 atgctaaacc aatcgtttat ccaactgtgc ctcttgggac aggcaggatc agaaatatgc
    10261 cgactgcgga acatacgagg gaaatgcttg atgaagcagt agcagtttat caaaaaatag
    10321 gaaaagaaat ggaaattatt taactaaatt tagttttttc gtctatttaa aattagagaa
    10381 catgttcacg catatattgt taatcatctg aagatagtag taaaaaagat cagggaggat
    10441 catcatgaaa aaagtattgg tgacaggatg tttaggacaa attggttcag agttgatcac
    10501 acggctacgt caagaactag gcagtgaaaa tgtcattgcg acagatatac gcatgcctga
    10561 agtcattgag aaaaacgata tctttgagat cctagatgta atggattacg aaaaaatgcg
    10621 cagcatagca gaacaatatc aagtggacac attgattcat ttggctgctc ttttgtcagc
    10681 tgttgcagaa gcaaaaccac aattagcctg gaatttgaac gtgactgggc tggtcaatgc
    10741 attggaagtc gctagagaat tccagctgaa attttttacg ccaagttcaa tcggcgcgtt
    10801 tggtccaaat actccaaaag atcatacccc acaagatact cttcagcgtc ctacaaccat
    10861 gtatggggta acgaaggtag ctggagaact actatgtgat tattattatg tgaaatatgg
    10921 cgtagatact cgtggtgtcc gtttccctgg attgatttct tatgcaacct tgccaggagg
    10981 cggtacgaca gactacgcag tcgatattta ttattcagca atcaaagaag gaaagtacac
    11041 tagtttcatt gatcaaggta catcaatgga tatgatgtat atgccagatg cgatcgatgc
    11101 cattataaaa ctcatgaatg cagatcctga tcgattggtt catcgaaatg ccttcaatat
    11161 atcggcgatg tcttttgacc cggaagagat caaagcatct attcaaaaaa tcataccaga
    11221 ttttgaaatg gattatgatg tggatcctgt acgtcagtca atagcagaat cttggccaaa
    11281 ttcactagat gtttcttgtg cgcaagagga atggggcttt tcgccgaaat acgacttaga
    11341 tgtgatgaca aaggatatgt tggaaaaact gaaagaaaaa ttggtagaga cggtataaaa
    11401 caagaaccac atccaaaaag ttttttatac ttttgggatt ggctatggta tgaaagggga
    11461 caagggatgg aagaatatca agccacgcca gtaaaagaag taaaaataga aaatgaatta
    11521 aaacgtacga tgggattttt tactgcttta tcgactgtta tgggcacagt gatcggtgca
    11581 ggggtatttt ttaaagccgc aagcgtagca gaagttacag gatccgccag tttgcatatg
    11641 ttttcttggt ttctcggtgg tatgatttct gtctgtgcag gactaacagg agctgaactg
    11701 gcagctgcta ttccagaaac aggagggatg attaaatata tcgaacggat ttacggaaat
    11761 acagcagctt ttttattggg ctgggcgcaa gtcgtgattt attttccagc aaatgtagca
    11821 gccttgtcga tcattttcgg tacacagttc gtcaatctat ttggtctttc ccagtcaatg
    11881 attgttcccg tagctgttac agctgctgta tcgatcctgt tgatcaattt tttaggttct
    11941 aaagctggag gggcttttca gtcgattacg cttgtctgca agcttattcc tttgtttgtg
    12001 atcgtcattt tcggtttgtt ccgacaagaa ggagtagact tccaactgtt tccgatccaa
    12061 gcaggagaaa atctttcatt tttctcagcc ttgggagcgg gcttgctagc aacgatgttc
    12121 gcatacgacg gttggataca cgtaggaaat atttcgggag aattgaaaaa gccggcgaaa
    12181 gatttgccaa aagcaatttc tttaggaatc atcggaatca tgatcgttta tttattagta
    12241 aatgcggtgt tcttaagaac cgcatctatc gacggagtcg caggaaacag caatgcggca
    12301 agtgacgttg caaaaatgat ttttggcggc ttcggtggaa gactggtcac agttggtatc
    12361 ttgatttctg tttatggaac catcaatggc tataccctta ctggaatgcg tctcccttat
    12421 gttatggcaa aagaaaacaa cctgcctttt agcaagctct ttgctaaact tcatgataaa
    12481 actaaagttc ctgtagccgc aggtatctta gagctagtca tcgctattgg catgatgatg
    12541 atcggtggtt ttgatacact aacggatatg ctgatctttg tcatttggat tttttatacg
    12601 atggtctttg ttggtgtcat tcttcttcgt aaaaaagaac ctgatttgtt tcgaccatac
    12661 aaagttccaa tgtatccgtt tattccacta gtagcaatca ttggagggac ttttatcgta
    12721 tcaagcacgc ttatcactca gaccttttta gcttcgatgg ggatagtatt gacacttgct
    12781 ggtatcccga tttatctcta tttaaaacgt agatagctaa aacattcgaa ttttattaga
    12841 actgcacttc tctgccaaac aaaatatttt atttaaatga ggagtacgtg gaagaaatgt
    12901 caaaagtact cgttaatttt gatcacgtaa atgtcgtttt gtctacgatg aaatagcaaa
    12961 gtaaaagaca gaaatcgggc tcaaccgatt tctgtctttt actttgattt gcgtaactga
    13021 actattcggc gtacgccgcg ataaatattt aataaaacca agcaaccaga aatgaaaatg
    13081 ggaagccaaa tcagatagac agggtagcca agcactttag acaaagcatg tggagtcatt
    13141 ttatccattt caagaagtgc ttgctgccaa gtctgaagat tgaagactag catgaaaccc
    13201 gaaataatca ggagaataaa tcctaaataa tgaattgtag ttctttttct tacataagcg
    13261 atcgtagcat aaaggaacag taaaaaaata ggtacgccaa aaacaaagta catattcagc
    13321 atctcgacac cccctttgtg tttatcttac tttactttga aagaaatgag aaataatagc
    13381 ctcgttactt gcttgaaaca gcaaaggact gctaagataa gagagataca aagaatcaag
    13441 gaggagtcat atggcaagtt atatcgaacc gattcgtttt gcgctgattg tttttccatt
    13501 tttagcctta gcgatctcat ctgttttttt tatttatgaa tatcgaaagt atggaacatt
    13561 tctagtaaca agggcagtta ttttgtattc tttcgttttt tatttattgt gtgcgtattt
    13621 tttggtgatc ttaccacttc cggcaaagga gacggttgcc caaatgacag ggccaaagat
    13681 ggagctgcat ttatttgctt catggcaaca tttcagagac aacacggtat tggtcttgtc
    13741 tgaacctagt acctacctgc cggcaatgaa gcagagtgta tttttggaac cagcttttaa
    13801 tgttttatta ctcataccat ttggcattta tttaagatat tatttccggc tttcttttat
    13861 gaaaacgatg attgctagtt tctgcctttc tctatttttt gaattgacgc agctaagtgg
    13921 actttatttt atctacccaa gaccataccg cttatttgat gtgaatgatt tagtgacaaa
    13981 tacattgggt ggaatcattg gttttgtcgt tacgccgtta tttacattta tgcttcctac
    14041 aagaagacga atggatgaac tttcttatga aaaaggaaag actgtctcat tgactcgacg
    14101 cttggtcgct tatttgatcg actggacatt tttatctctt attaatttgg tagcggcgat
    14161 tttctttaaa attgcgttcg attcaggggc agttacttct catatctggt ggcttttatt
    14221 agaagtaggg gtatacttta tgattgtacc gtttataacc aatggatgga cacttggcaa
    14281 aaaaattgtg cgtatccgtt tggtggaaga agaccgcgaa cgaatttctt taaaagcatt
    14341 gatgatccgc tacgggtatc tgtactttct tttttatggt ttaagctggt tggggcagtt
    14401 gcttcagcaa atcatgaatt cgacaacgaa tacggtgttg cagatgagtg cagtattcat
    14461 ttctctcggt gtcttgttac tacaaggtat ttttatttta aatatcttgc tatcattttt
    14521 gagaaaaaaa cggattctgt tttatgaaaa agctagtcac acatatactg ttagcacgat
    14581 tggccaaaca aaggatgatg attctcgaaa ttagctgagc aaaaacgctt tgtctgtgtt
    14641 ataatgccaa agtcgtgata atttaaagag aagaggtgca cgaatgaata attttgatat
    14701 attccgcttc aaaccgttta tcaatgaagc gttaaccgca aagggcttca aacagcccac
    14761 agaggtccag gaacgtttga ttcctttaat tgcaaaagga aaaaatatca ttgggcaatc
    14821 tcaaactggt acaggaaaaa cacatacgtt tttgttgcct ttgtttgaaa aaatcaatgc
    14881 agaaaaagag gaagtacaag ttgttattac agctccaagt agggaactag ctgcacagat
    14941 ctatgaagca gctacacaaa tcgctaaatt ttctacaccg gagatccgtg tctcgaattt
    15001 tgtcggcggt acagacaaac agcgtcaatt aaatcgtctg catcaccaac agccacaggt
    15061 tgtagtaggc acgcctggta gaatattaga catgatgaat gaacaagcgc tgaagattca
    15121 caccgcattt gcatttgttg tcgatgaagc ggatatgaca ttggatatgg gttttttaaa
    15181 tgaagtagac caaattgcaa gtcgattacc agaaaagctg cagtttttag ttttttctgc
    15241 cactattcca gaaaaacttc gtccattctt acgaaaatac atggaaaatc caatcattga
    15301 agaaatcaaa ccaaaagcag ttatttctga agacattgaa aattggctga tttcaacaaa
    15361 aggtaaagat gagaatcaaa tgatttatca actgctgaca attggtcatc cttatttagc
    15421 aatcgtattt gccaatacaa aacagcatgt ggatgaaatt gccgattact tgaaaaacca
    15481 aggcttgaaa gtagcaaaga ttcatggaga catcacccca agagagagaa aacgagtgat
    15541 gcgacaagta cagaatctgg agtatcaata tgttgtagcg actgatttag ctgctagagg
    15601 gatcgatatc gaaggtgtct ctcatgttat taatgcagaa attccagaag atctagactt
    15661 ctttatccat cgtgtcggac gtactggacg taatggctta aaaggaacgg ccattacgtt
    15721 atattcgcca aatgatgagc aagccattga tgcgatagaa aaattgggcg tctcatttga
    15781 accaaaagaa atcaaaaacg gggaaatcgt caaaacatac gatcgtaata gacgtgccaa
    15841 acgagaaaaa tcaaaagatg tattagatcc cacgctgatt ggtcttgtga agaagaaaaa
    15901 gaaaaagatc aagcctggtt ataagaaaaa aatcgattgg gcaattgcag aaaaaaataa
    15961 aaaagaacgc aaaatcgaac gcagacagca aagccgtact gctcgtaaaa ataagaaaaa
    16021 ttcacataaa taaaaaacaa gaggttgtga caaaaggcgt gtcacaatct cttgttttta
    16081 agctagactt tttttcattc cgatacaaag tacttcatct tcaataaaag gttctgtgca
    16141 agtatgatac ccttgttttt cataaaaatc tacagccgtc atttcagcta ctacataact
    16201 agtctgacaa ccttctgaaa ccgcccgttt ttcagcagct tcaagcaact gaccgcctaa
    16261 cccttttcct cggtattctt ttgctacgca aaatcgttcc ggcttgaagc aagtaggtgt
    16321 tttcttttca taacgcaaag tagctactgg tcttttttga tcaagaaata gataataggt
    16381 ttgttgcggg atatcttttc cgtcgaattc tagttctgca ggaatccctt gttctaatac
    16441 aaaaattgat atctcaaaaa gcagctagct gcgcgtatcc attcatccgc tccaactatt
    16501 tttttcatca tattcctcct tgtcatgaat agcagagatg tattgattcc tttattctta
    16561 tcgtaccaaa aatcgtggag tatgaccaaa aggaaatgaa aataaaaatt gagatattga
    16621 cagtgatcaa tagaatgcgt atactaatta agtacgaaaa ggatatgatt tgttttttaa
    16681 gactcacaga aaattctttc ttggctgaaa aaagaatagt cactaatcaa attgctccct
    16741 ttttctattt tggtagaaat accgacgcag ctcagcgtta tcgagtttaa gaggtaatga
    16801 ttacacatca ttgcaatcaa ggtggtaccg cgagttttcg tccttggctg tgatggtgtt
    16861 tttttgtatt taatttatag aaagcaggta atgacatgaa acaattaagt agtgcacaag
    16921 ttcgacaaat gtttcttgat ttcttccaat ctaaaggaca tacgattgaa cctagtgcgt
    16981 ctttgatccc cgtgaacgat ccaaccttat tgtggatcaa ctctggtgtt gcaacattga
    17041 aaaaatattt tgatggttca gttgtgccag aaaatccaag aatcacaaat gcccaaaaat
    17101 ctattcgaac aaatgatatc gaaaatgtcg gtaaaacagc acggcaccat acgatgtttg
    17161 agatgctggg taatttttcg attggtgact atttcaaaaa tgaagccatc cactgggcat
    17221 gggaattttt gactgatcca aaatggatgg cattcgatcc agaaaaactt tatgtaacag
    17281 tttatccaaa agatacagaa gcaaaacgca tctggcatga agaagttggc ctgccactag
    17341 atcacattat tgatgtagaa gacaacttct gggatatcgg tgcaggccca tgtggtccgg
    17401 atacggaaat tttctatgac cgtggagaag cttataacga tttagcagaa gacgatcctg
    17461 aaaactatcc aggaggagaa aatgaacgtt atttagaaat ctggaactta gtattttcag
    17521 aattcaacca taagcctgat aacacatttg aaccacttcc tcataaaaac attgatacgg
    17581 ggatgggatt ggaacgcatg gtctcaatcg ttcaagatgc gtcaacgaac tttgagacag
    17641 acttgtttat gcctattatc cacgccgtag aaaagctaag caaacaagta aaatatggtg
    17701 aaaacccagt aacagatatt tcttacaaag taatcgcaga tcatattcgc gccctttcat
    17761 ttgctattgg cgacggtgca ttgccatcga atgaagggag agggtatgtc ttacgccgtt
    17821 tgcttcgtcg tgctgtcatg catgggaaaa aattaggtat tgaacaagca ttcttatatc
    17881 gtttagtccc tgttgttggg gaaatcatgg taagttatta tcctgaagta cttcaacaaa
    17941 aagaatttat tgaaaaagtt atccgtacag aagaagaacg tttcaatgaa acaatcaatg
    18001 aaggtttgga aatcttaaat caagtgatcc aagaagtaaa aaacgaaaac gggacgactc
    18061 tagcaggaaa agacatcttc aaactatacg acacatatgg cttcccggta gaattaacgg
    18121 aagaggtggc agaagatgaa ggactgaaag ttgatcatgc tggttttgaa aaagaaatgg
    18181 atgcacaacg cgaacgtgct cgctctgccc gcagtactga aaaatcaatg ggggtacaat
    18241 ctgctttatt gacagatatc aaagtagaaa gtaaattcat tggatacaca gaacaagaat
    18301 cagatagtca attattggtc atcgttaaag gtgagtctct tttgaatgaa gtcgcagaag
    18361 gtgaagctca aatgatcttt gatcaaacac ctttctatgc agaaatgggt gggcaagtag
    18421 cggatcaagg atggatcatg aatgaagtag gcgaaatcgt agcagttgtt acagatgttc
    18481 aaaaagcgcc taatggtcaa tttatgcaca ccgtagaagt aaaagcacca atgagtgaag
    18541 gacaaaccta tcatctattg atcgatcaat cgatgcgcaa tcggatcatc aaaaaccata
    18601 cagcaaccca tctactgcac aaggcattga aagaagtact tggcgagcat gcaaatcaag
    18661 ctggttcttt agtcgctcct ggacatttac gattcgactt tacccacttt ggtcaaatca
    18721 cttcagatga attagctgaa atggaaagaa tcgtcaacga aaaaatctgg gaagctatcc
    18781 cagttgaaac aattgaaaca gatatcgata cagccaaaaa catgggtgca atggctttgt
    18841 ttggtgaaaa atacggacat gacgttcgag tagtaaatat tggcggctgg tcaatcgaac
    18901 tgtgcggagg cacacatgta gcaaatacag aaaacatcgg aatcttcaaa atcgtttctg
    18961 aatcaggaat tggtgcaggc gtgcgacgaa tcgaggctgt aacaagtaaa gaagcgtacg
    19021 aactacttga agaagaagaa aaacaactaa aacaagtagc aacaattgtt aaatcacctc
    19081 aattgaaaga agttgtttca aaagcggaac aattgcaaca gcaagtaaga gagctacaaa
    19141 aggaaaatga acaattagct agcaagttag cgaatcaaca agctggtgat gtattcaaag
    19201 atccagaaac gatcaatgga aaaacaatca ttgcagcaca agtaaaagtg aaagatatga
    19261 atcagctgcg tcaattagct gaccaatgga aacaaaaaaa tatttcagat attcttgttt
    19321 tagcaaacga acaagaagga aaagtcagtc tgctagcggc aatgacgaaa gaatcaaatg
    19381 aagctggact aaaagctggc gacttgatta aagcaatcgc accgaaagta ggcggtggcg
    19441 gtggtggacg tccagacatg gcacaagctg gcgggaaaaa tcctgctggt attccagatg
    19501 cacttaaagc tgcgaaagaa tgggttgcgc aacaataggg cgtttacgtt tgacagaaaa
    19561 acaaaagttc aaacattgaa cgaaacatga ttatgggggc gaagacaaac aagtcttgcc
    19621 ctcatacttt tttcaattgg atggcgcatc taatcaaatg gtcggattcg gatggtcttt
    19681 agttgacgaa agtgacagaa atttttacga ttagtataaa taaaaagggg tgaaaaaatg
    19741 aatagtagaa ataagacctt tcgactagtc ttacgtgcga tcttgttagc aatcatcatc
    19801 gtacaagcga tggttccttg gctggggttt attccattag gattcattag tttaacaatt
    19861 atccatatca cagtaattat tgctgcagta gtattaggcc caaaagatgg catggtgatt
    19921 ggcttgtttt ggggaatcgc tacaattgtt cgtgcctatg ccatgccaac aacgccattt
    19981 gatacattag tgttcacgaa tccgattatc tcggtagttc ccagagtact tgttggctta
    20041 gtagcaggct tggttttcca ctggatttat caaaaaaata aatcaataac gatcagctca
    20101 gcctttgcag gggtactggg ttcattggtc aatacagtct tagtgttagg attcatggga
    20161 ttattttata caggtgcaac ggcagatgcc tatggtgtag actctgctct tttatttaaa
    20221 accttagcag gggttgcagc aatcaatggg ataccagaag caataggtgc gggaatcata
    20281 acaccattac tagcaaaagc gttatttgca gcaacaccgt tgaaaccaga ataatgacca
    20341 tcttttaata gtaataggac gcacgataga tagaaaaagg aagagaggag aggaaaacaa
    20401 tcgtcctctc catcttttta cctgagcaac aagcaaacta gaaggggtaa aaaacttggc
    20461 taagaaacaa gaagaaaaaa agaaacaaat accgcaacag cggacaaaaa aaggagtact
    20521 agcgggtatc ttaggattgg ttacgaatct gatcttgttt gtctctaagc taatgatcgg
    20581 gcttttttca ggaagtgtct ccattatggc tgatgcagtg aacagtctat cagatactgc
    20641 ttcatccata ttgactttga tcggatttaa aattgctgca aagccagcag atcaggaaca
    20701 tccttatgga cacgaacgct tcgaatatat cagtgggtta tttgtttcga tcatcattac
    20761 ttatgtagga tttcaatttt tagagtcttc tgctgaaaaa atcatccatc cagaaaatgt
    20821 ctctttaaca cccatcgtct tttttgtcct aatcatttcc atctttttga aatttgcaca
    20881 aggcaaaatg tatcaggtta ttgcgcaatc cattgattct gaaacattga gagctacagc
    20941 aacagatagt tataatgatg tctttactac actggctgta ttgctttcag cagcaatcga
    21001 atgggtaact ggatggcgta ttgatggata tataggtttt gctttagcat tatatatcct
    21061 ttatagtgga ttgacgatgc ttcgaaattt tgtatacgaa ctattaggaa gtcgcccaac
    21121 gccagcagag attgaagaaa tggaagagca tttaaatgct tgcgatgata tacttggcta
    21181 tcatgattta ttggtgcata actatggacc caataaacgc tttgcatcag tccacatcga
    21241 agtagatgaa tcaatgaatt tgaatgtggc tcataagatt attgatctga tagaaaaaga
    21301 ctttaaaaag tctttggatg tggaacttgt ctgtcacttg gatccaatgc ctgttgataa
    21361 cgaaaaatac tataatatct tgcggcagtt aaagcaaatt cttcttgaaa caggtacagg
    21421 gcttaatatt catgatttta gagtaattca tcaaggaaaa acgctgcaat ttgatgtagt
    21481 ggtccctgag aaattccagt ttgttgatac agaactagaa agaatcttac gtagtcaaat
    21541 agaagaaaaa atcggagatt accaattgga aat
//
LOCUS       DAZUMK010000039        20926 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000039 DAZUMK010000000
VERSION     DAZUMK010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.039"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            30..3308
                     /locus_tag="ACG808_001387"
     CDS             30..3308
                     /locus_tag="ACG808_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFS5047018.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3849..5756)
                     /locus_tag="ACG808_001388"
     CDS             complement(3849..5756)
                     /locus_tag="ACG808_001388"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFS5047019.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5886..6761
                     /locus_tag="ACG808_001389"
     CDS             5886..6761
                     /locus_tag="ACG808_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFS5047020.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6841..7815
                     /locus_tag="ACG808_001390"
     CDS             6841..7815
                     /locus_tag="ACG808_001390"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFS5047021.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            7995..9140
                     /locus_tag="ACG808_001391"
     CDS             7995..9140
                     /locus_tag="ACG808_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFS5047022.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9277..9621
                     /locus_tag="ACG808_001392"
     CDS             9277..9621
                     /locus_tag="ACG808_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFS5047023.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9892..11178
                     /gene="efmA"
                     /locus_tag="ACG808_001393"
     CDS             9892..11178
                     /gene="efmA"
                     /locus_tag="ACG808_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFS5047024.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11316..11915)
                     /locus_tag="ACG808_001394"
     CDS             complement(11316..11915)
                     /locus_tag="ACG808_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS5047025.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12319..13440
                     /gene="mnmA"
                     /locus_tag="ACG808_001395"
     CDS             12319..13440
                     /gene="mnmA"
                     /locus_tag="ACG808_001395"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFS5047026.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLIYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13686..15107
                     /gene="arcD"
                     /locus_tag="ACG808_001396"
     CDS             13686..15107
                     /gene="arcD"
                     /locus_tag="ACG808_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFS5047027.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15121..16239
                     /locus_tag="ACG808_001397"
     CDS             15121..16239
                     /locus_tag="ACG808_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFS5047028.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16299..17591)
                     /locus_tag="ACG808_001398"
     CDS             complement(16299..17591)
                     /locus_tag="ACG808_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFS5047029.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17741..18301
                     /locus_tag="ACG808_001399"
     CDS             17741..18301
                     /locus_tag="ACG808_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5047030.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18430..18807
                     /locus_tag="ACG808_001400"
     CDS             18430..18807
                     /locus_tag="ACG808_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFS5047031.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18740..20812
                     /locus_tag="ACG808_001401"
     CDS             18740..20812
                     /locus_tag="ACG808_001401"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFS5047032.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
BASE COUNT         6779 a         3799 c         4474 g         5874 t
ORIGIN      
        1 ctttctaatt tgttacggga ggaatacact tgttaaaaaa acgtataaaa tacagtgcgc
       61 taatgatgaa tctccttatg ctcagtaccc cggttttaat ttctgtcgtt gaagttgctg
      121 ctaacgaaca acagtctgtg aacgaagaaa atgcacacgt gaacattgaa gggatgtcaa
      181 caatggagga aataccaaga actagtgaag atatggatac ggaagaagga aatgatccta
      241 tgatgaaaga atcagaaaat gaaaagacaa aaaagcaatc tgaagaagca aatgaagagt
      301 ggaaaggggt tgttttcgga gaaagcacaa gtgctagtag gtatgccatt gaaccattag
      361 aagacggcgt gcggctctct tccacgaata acggcggaaa attccagtca agcggatcag
      421 atggaatgac ctattattat caagcaatcc ccaaagacat caactttagg atgcgggcga
      481 cgatcgaaat agaatcttgg acctatacga atgcccaaga aggatttgct ttaatggtaa
      541 gagacgcagt accaaaaaat catttgtttg ggcaggcatt ttattcaaat tcatttgcca
      601 tactaggcag tcggatcgaa tatgtctggg actcggatag acaagaagtg gtcaacacga
      661 gcggcagcaa atatacgatg cgacttggat tggggacacg ggcaatcaca ggaatatcat
      721 ctgaagatcc gcaagctgcg ccagctcctg ggagtgtctc agtggaaaca acacctttag
      781 agacttcagc agggttcttg gaaaaagaaa caggttctta taatatattc ggaaacggtc
      841 atgggaatct gtttccggcc acgaactcga ttactaaact ggacgtagag atgaaaaaaa
      901 cgaatactgg tttagaggca tattattacg atcctgaaac aggagaagaa atggcttctg
      961 acatcatgta tgactgggaa aaattatacg cttcagatga atcggttatt tatgcaggat
     1021 ttgctgcagc aaggaatatg acgatcaagg tagaaggaat cgaagttgct tactcagatc
     1081 ctgcgacaga tcctcctggg caagaaagac ctactcgctt gattgatccg atttacactg
     1141 tgacctctag taatcattca ggaaaccaag atcatactat gtcctttaga gcaaatgctg
     1201 acggattatt gacaattaaa aataaagcaa caggagaggt gcttaagaac gcaaaaaact
     1261 tggctatcac tgcgaatcgg gaatttgatt atcagacaaa actgatggaa ggaacgaacg
     1321 aatttacttt cagttttacg ccagataaaa actatccgcc agaagaatac gtagagatga
     1381 ctagttatga aacaaaagag attcttcata cagtggacta ccgtaaacct aaatattcga
     1441 cagtttatgt aacaccagaa gggtcagatg caggaaacgg aagcagacaa aatccattat
     1501 ctctgacaca agcattgaga tacgcatatg ctgggcagac aatcgtcatg gcgcctggaa
     1561 cttattcctt cgaaagagga ttgacgattc caaaaggcgt taacgggaca agagaaaacc
     1621 cgattcagtt gattgcagag aaaaatccag aaaacaagcg tcctgtactt gattttaaag
     1681 gaactggaaa tgggatgaca ctgttcagtc actactgggg tttacgaggg tttgacatta
     1741 caaatagtgc agctatgcaa aaaggattac agatttctgg aaatcataat ttgatcgaag
     1801 agattcatgc ttatcggaat ggaaatacag gaatccaaat cagcggtagc tcaaatgatc
     1861 ctttcgaagc atggcccgcg cataacttag tcaagaactg tacatctttt gaaaatgccg
     1921 atccaggatt tgaagatgcc gatggatttg cggccaaact gacaatagga gaaggaaatg
     1981 tatttgacgg ttgtatcgcg tatcataatg cagatgacgg ttgggacctt tttgctaaaa
     2041 atgaaacagg ttcgatcgga aaagtaatca tcaaaaactc agttgcttat cgaaacggct
     2101 gggtaccagg aattgaggga gaaggtaacg gtaatggatt caaaatgggc ggttcttctt
     2161 taacgggccc tcatgaattg atcaatagtt tgtctttcga aaatcttgcg aaaggaatcg
     2221 attccaatag cggaacagat attatcgtca atagcagtac aagttttaat aacggttcat
     2281 ataatgtggc tctttatacc tcttcagcag gaaacacaga ctaccatgct tctggtatct
     2341 tatctttccg aacagagaat cttgagatga gagagcagat ccgcccattg aatcaaaacg
     2401 aagatcaaat ctaccactcg ttgaactact attggaatga acgagagaaa caatcagaaa
     2461 atactcttgg acataccata tcaaaagatt ggatcgagtc tctaaataca tgttcgaaag
     2521 aaggtcagca gcctgttacg cgctatgaaa atggaagtat ccatgttcat ggactgatgc
     2581 agattaaacc ggggaatcgt caaacagaaa aggaaagagt ggatggtctt ccattatctg
     2641 taggtgctat ttttgacgga gaaacctcac cgtcttcgga atatccagag tatgcaatcg
     2701 tacaagaacc aaatcagaca aaaccaaatg aacaaaataa tccgattatt atacaagaag
     2761 caaatggtgt ggcggagaat gcagaaggaa atatcattga actgaagcct tttacagttt
     2821 ttgtgtcaac tttcacacca aatcaaggaa cgcctgtctt tgaagatatg cgttatttcc
     2881 cagaaaagat ccaaatcgtt gaaacacagt caagtgctca attttctaat cataccgcta
     2941 ttttttcagc accttcaaaa ggagaatata cgctagaagt cacttatcgt tcggaaatat
     3001 ttgatgggga aaaatggtct gctcatacag aatctgttac ccagcaaaaa gcgatcactg
     3061 tgcgagaaac tgccgatgtg cccccgggaa atgacaaacc agaaagagaa aatccaggaa
     3121 atgaatcacc aaatggaaaa ccaaatggaa aaccttctca tccggatagg acagcaaaag
     3181 aaagtgaaaa aaatggcact caaggactcc caaaaacggg agaatttaat aacccgttgc
     3241 tagcattagc tggaggaatt ttgttaatcg gtgtagttgt ttatgtcatg aaacagcgaa
     3301 aaaaataagt ggaatttctc cttttatgat gtaaaacaat tgactgagga aagtaaaaat
     3361 agaaggaaat gagaccaata gatgtctcac ttccttctat ctttttatag atgaggttct
     3421 ccttgctgct tttgattggc tgttcttatt acttgatgca ggacagcttt ttcacaatct
     3481 ctccactaaa cggtgttcaa tcagctgacc ctcggtatta gctcaacaaa cggcaaaata
     3541 tgaggaacta tttttccgtt tttttctcta atactcaggt caggacgctg atttcacaac
     3601 ctctcgataa acggtgttca aaagccagtt tctcggtagt aagtcaaatt tatgcaaaaa
     3661 tgtggaaagc cattttcaca aaatttttct tactacttga aactaagcag ctttttcaca
     3721 acctctaata catggtattc cagaagtaac ttcttcgaaa aataagccga aatgtcacaa
     3781 aaatttgaga agcgattttc ccaaattcct tcttatttct tgaagctgac cacttctggc
     3841 acaacctctt accagattcg cactcttttt tctggcgcta gatacatagc gtcttgttcc
     3901 gtgataccaa atgtttcata gaattcgtca aggttttttg gttgcacgtt tgctcggagt
     3961 ttagcagggg catggacatc gatttgcagt aaaagttgtt ggtattcttt ttttgctttt
     4021 gtacgccaaa tcgttgccca attgacaaag aattcctcga tagaaagatc tgattccgtt
     4081 tttgctgctt ccaatgcaca gcttagaccg cctgcatcag caatattttc cgaaacggtt
     4141 aattttccgt ttacttttcc gtcagcaaaa ggcagaccat cgaattcttc gatcattgct
     4201 tgtgctttgt tttgaaattg tgtaagatct tcttctgtcc accagttgtt caagttgcca
     4261 tattcgtcaa ataaagcgcc gttattatca aatgcatggg aaatctcatg agcgataaca
     4321 gctccgattc caccataatt agcagagctt gattgttcta gactgtaaaa tggtgcttga
     4381 aggatcgctg ccggaaagac gatcacattc cgaaatggat gataataagc attgaccgtg
     4441 tcagcactca tttcccattc tgaacgatcc actggtttgt tccatttgct gaaacgatct
     4501 tttaatgtga gtttgctgaa aacaagagta tttgaaagca aagtaccacc ctgatctgca
     4561 ggtactgttt tgaactggtc gaataatgct gggatcttat cggggtagcc tacttggata
     4621 cctaagttat ttaattttat gacggctttt tcttttgttg catccccaag ccagtcattt
     4681 tcttctagac gttttttata aacggcgatc atttttttca ccatttgttc tacgtctttt
     4741 ttagcagctt cgccaaaata tttttttccg tagtaatcgc caactacttg atcgaattgt
     4801 ccagaggcaa ggtaatacgc tgcttttttc tgaggcattg cttcgtctgt tccagacaat
     4861 acgcgagaat agattccgct gacttggcgg aattcttctg atagataacc actaagagaa
     4921 cgtactgttt taatgatcat ccagcttttg aataacggaa aatgagcttc tgtcaggatg
     4981 tcattcaaat gatcgaagta cactggatca gttacgatca ctttatctgg agttgtccca
     5041 atcaaacccg tgacaagttt tttcaagtcc agtttatctg tgtatttaat aaatgtatca
     5101 aaagattgcg gattatacat tttgctgtaa tctgcgtttt cttctgcact ttttacatga
     5161 ggagcaatca atttatcaaa ttgcactgct tgttccgtga ttgcttctgc ctgacttttt
     5221 tctttgccgg caagctctaa taactgaaca gccatatcaa agaaaacgcc tattagttgt
     5281 gcaccgttgg gatggtctga tgcataatac gtcttatctg gaaggaataa agaaggcgga
     5341 taagcaaaga aggcgttcgt ttgcgcattc ttcatatctg catctacatc cagattaaat
     5401 ggcaatggta atgtatccaa cagccattct ggaaattgct tattcaattc gtcgaaagac
     5461 tgcaatgatt caactttttc gattataggt tgaagcggtt gagcatgcaa ctcatttctt
     5521 cggtcataat cgtttgccag ttgataatat gcaaggaaat gtttcatttt ttctgaagac
     5581 actttttctg gatgggcaag cagatcgtct gtttctccca tcaaaagttg gtcgatcccg
     5641 tcaactaaat cttggaaacc gcctgtagct ggtttatctg cagggatttt tgctgttttt
     5701 tgccattctt cattgactgc ttcatagaaa tcttgtttca atcgttcttt gttcattcat
     5761 cgatcgctcc ttttttgttt cgtgtgccca ttatcttaca aaatgaagga attaacaaat
     5821 agaacataaa aaattgaaaa ttcgaacaat caaactgatt tgtttttttc aatccttcac
     5881 ttataatgaa aaaaaggagg cgatcccaac tgaatcaagt agtggataaa cctttatatt
     5941 tcaaagtaga taaaaaaatt aaaaaattag ccagcactca gcagctgcag tcaagaaaaa
     6001 gtaaattgct ttttttagct ctggtttttg aagatcaaag ttatgtgatt atcgatcaat
     6061 caggacatcc ggtagaatat tctccagcaa aatatactta tcaagagggt ctttcttgta
     6121 aaaaatggaa gctgatcaat gaagagccaa tcgagttatc tagatggatc aacagaaaag
     6181 aagatattcc cgttttgatc gaagaaaaaa ggtcaggaaa ggaactcgca aattgctggg
     6241 tgggattgcc agaagaacgt tttttgaggt ataagaaatg ggctaccccc tcaggctatc
     6301 tttgtggtac ttacgcggca gcagttctcc ttgcctatta tcaagactat cgtaaggggt
     6361 ggatgcttcc aaatgagatt cgcaagaaaa atacggctga ttcgttggtt ttgacgaagg
     6421 cattgcgtag ccagatccaa ccactaggac ttcctacgat tccttttcag gttagtacag
     6481 ggatttctag ttttttaaag aagaatggca atcatgagcg tgcacgagca actcttctag
     6541 gttcatggca gcgagctaca aaacgtatac gtgaaggcaa acctgtgatg attggtattt
     6601 tgaaagtact tggcagtaca tatggcaatc attgggtgac agcgtacgct tattttgaaa
     6661 ctgaaacagg agaacgttat tacaaagtac acgacaactg gggcgattat cataaagtca
     6721 ttcctgccag ttggagtaac ggaacagttt cacttcctta agaatttgct gaaattaaca
     6781 gaaaatcttg gaaaaatatg ttaaactatc gaagaataga gaagttttgg ggaggactac
     6841 atgagtaata agtatcagga tgacacgttg aagattttta gtttgaatgg caaccgtcca
     6901 ttagctgaaa aaatcgcgaa agtgttcgga acagaattag gtaaaagcgt cgtgaagcaa
     6961 tttagtgatg gcgagatttc catcaatatc gaagaaagta ttcgagggga tcatgtgtat
     7021 atcgttcaat caacgaatgc acctgtcaat gattactata tggaactact gatcatgatc
     7081 gatgcaatga agcgagcaag tgcgaaaacg atcaatgtcg tattgcctta ctatgggtat
     7141 gctaggcaag accgcacggc gaaacctcat gaaccgatca ctgcgaaatt gatcgctaac
     7201 ttgatcgaag aagcaggggc aactcgagta ctgactttag atctgcacac ggttcaagta
     7261 caaggattct ttgatattcc tgtagataat ttatttacga tgccgttgtt tgctcattat
     7321 taccgtcaat taggtctgac gggagatgac atcgtagttg tttcgccaaa aaacagcggt
     7381 gttcagcgtg cacgtagtct ttcagaatat ttgaacagca cattagccat cgtagatcat
     7441 gcagatgatg cttcaactga gggtggttat gtgatcggtg atgttcaagg aaagacatgt
     7501 atcatggtag atgatatctt gaacacagga gcaacgcttg cacgagcagc aaatgtatta
     7561 aaagaaaacg gcgcaaaaga agtatatgcc tgtgcgtcac atggattatt gtccgatcat
     7621 gcgaaaagta ttttagacaa tgcaccaatc aaagatattt gtatcactga ttctgttttc
     7681 acagaagaaa atcgtcatcc agaaaactta acaatcgtta catgttcaga attaatgggt
     7741 gaagcggtca aacgcatcca tgaaaataca ccgatgagtc cgttattccg tctggaagaa
     7801 aaagaattcg aataaacaaa aaaaagaggg aaagaagttg tgacagaagt gagcagcttc
     7861 aaggagctgc gacaagactt ttgtcacagc tccttttttc tatgcccatg aaacgtcctc
     7921 tagacaaaaa tgagctcttc tttggtttgt tcgctatttt atgctagaat gtgcctagca
     7981 ataatttgga agggatgaat gaagtgaaaa ctatttatct ggatcatgcg gcaactaccc
     8041 ccatgcatcc acaagtaatt gaagcgatga cggcaatcat gcaagacacg tttggaaatc
     8101 cttcaagcat acatggattt ggtcgccacg cgcatgaaaa attggaaaca gcaagacaaa
     8161 tcattgcaga cagtctgcaa gcaaaacctc acgagatcat ctttaacagt gggggaacag
     8221 aaggggataa tacagctatc ttagaaacag cgttctcgcg tcaaaaagaa ggcagacatc
     8281 taatcactac agctatcgaa catcctgctg ttcttaatac catgaaatat ttagagacaa
     8341 aggggtttga cgttacatat ctgcctgtag atgaaaaagg gaatctgtcg atcgaagagg
     8401 tcaaaaaagc gttacgagaa gatacgatct tggtctctgt gatgtacggg aacaatgaaa
     8461 tcggaaattt gatgccaatc aaagaaatcg gtgaactttt agcggatcat ccagcttatt
     8521 tccatacaga tgcggtacaa gcgtacggaa accaaacgat ccatccagaa gaattaggaa
     8581 tcgatttatt aagtatctct gctcataaaa tcaatgggcc aaaaggcgtc ggtttcttat
     8641 ataaacgaga cggagtaaat atccctgcat tgcttttagg cggggagcag gaagaaaagc
     8701 ggcgtgcagg tacagaaaat ttagcaggga tctggggtat ggcaaaagca gttgaactgc
     8761 tgtcacctga agaacgtgaa gcacgtaaca acaaatataa aggattccag caattgattc
     8821 ttgatacatt agaagcaaac gaaatccctt atcaagtaaa tggtgattta acgaataaac
     8881 tccctcatgt gttgaatctt cgcttaattg gcgaagcaaa tgatttgcta ctgatgaagc
     8941 ttgatcttaa tggggtcgct atttctactg gttcagcttg tacagcagga aatatcgagc
     9001 cttcacacgt attagaagca atgtatggag aagatacacc gatactaaaa gaatccatcc
     9061 gtatcagctt tggtttggga aataccgaag acgacatcca gtatttcctt gatcaattag
     9121 ttcgtgctgt caaaaaataa ctagtgaagt cattcttata tgaacagatg tctgagagaa
     9181 cgccttcatt ttttagggaa cttaactgaa aaacctagta aaaatcagga taaactttta
     9241 taataaagag aaactaattt tatcgaggtg aaaatcatgg catttgaaac aacagcaacg
     9301 gtactgggcg caccaattag ctatcgtttg catcctgatg caaaaagata tacattgcgc
     9361 gataatggat ttacagaaac aaacggaggc aattatcaat tgatccgacc attagatgcc
     9421 acgccgcaaa gcaaagaagg attcaaatta aaaatcactg tatcaaaaga tattcaaaca
     9481 ttgaaaatgt cgatcactac agcaaatgga ttgaaagcag tcaatatttt caaagatcca
     9541 aaacagaaaa tgagccaaga taaattttat tttctgatgg atgggatgat tagccgtggg
     9601 ttattcgaaa aagtcgaatg atctgtggat ataaaaagac aactagtttt gaaacggcct
     9661 attgttagat gactaacttt gtaaggacga cgcaaattag ttgtcttttt tatttttaaa
     9721 tttaacacat gttgttattt ttaatatttt gctgatttat tgtttctaag taaagaaatt
     9781 taaattgaaa cgattgttat ttagggaaaa aggtattttt tgaaaaaaga atcccgtata
     9841 atgattgttg tgcaaacact atacaaaatg aaaaagaaaa gagggatgtt tatggaaaat
     9901 gaacagtcgg tcgttttaac gaattggaag agaaattatt tgttcttttt atcgggacag
     9961 tttttgtcag gaatcactag tatggtcgtc caatatgcga tcatctggta tttgacaaga
    10021 gaaaccggtt cggcgacaat tttgagtttt gctactttac taggaatgat tcccatggtt
    10081 ttactaagcc catttgtagg accgctagtg gatcgctggg ataaaaaagc tcttttgatt
    10141 gtcacggata ttattgtagc gattttcgcg cttattttag cagtagtcgg aacgatttca
    10201 gaatccttcc cgatttggct agtttttgtg tctttgttta tgcgttcggt cgcacaaaca
    10261 ttccaaatgc caacgatcca gtcgattatg ccgacaatcg ttccatcttc tcacataaca
    10321 aggacgaatg gacagttagg aatggtccaa tcagcgaatt tcatcattgc gccagctcta
    10381 ggtgcggctt tgttttcagt cgttcctgtc aattacctta ttttattaga tgtattgggg
    10441 gcagtttttg gtgtcggctt attgattttt gtgaagatcc caaaagtttc tcctgaaata
    10501 ttggaagttc cgcttactat ttttaaagat gcaaaatttg ggctgcagca attgatggat
    10561 aataaagggt tatggtacat aacgatcaat ggtgcatttg tgatgctgtt gttcatgcca
    10621 gcaatcagtc tgtatccatt gatgacgcta gattactttg gtggttccgt tggacaggcg
    10681 ggagcagtgg aagtagtcta cgcagtcgga atgctcttag gcggtgcact gatcagtttt
    10741 ataggaacat ggaaagaccg aatgaaacct attattatag cctacatcat tatgggcctg
    10801 acgatcggtg caagcggatt ggttccaaac gatagtcaag ggttcttgta ctttcttatc
    10861 ttgaatgcag gagcaggttg tgcaacgcca tatttcaata cactgctgat ggcaatgatc
    10921 cagcagagtt atgaatcgaa tgttttaggt cgtgtcttag ggaacttcaa ctctttgatg
    10981 aacctggcag gtccaattgg attattattt gcaggaccat tagctgatcg tttaggggta
    11041 gaaaaaatgt tcttgttttc cggaatcggg attttattat gcggaatcgt tttattttta
    11101 acgtcagctg cccgaaatta tgataaagaa ttacagaaaa aactggtaaa agaacaccat
    11161 gagcaaaaag acgaatagta tggaatggag ttgtgacaga agtcttgtta caactccatt
    11221 tctgatccta atgttcctgt ggcaataagc ctactcaaat gaaaaatatg agtaattatc
    11281 tttatttgtt taatacttga tatagggcag ttttttcacg acctatcgta cttcctcttt
    11341 gcttcataga tttctttgat ttgttgctca gataacggag tgccaaatgg tagtttttcc
    11401 tttaagagtt ctctttccgg tgcgtctacg cctacattga catcgttctc gataggtacg
    11461 ctgtaatgat ggatatgtcc atgaagattg cggatagtgt cgttaggccc gagcaacatc
    11521 ggataatgag tcaagtaata ttgttgatgg tcgaatttaa cgatggcacc tacatcataa
    11581 aaataaaatt tgggacttcc gtcagtaagc ctaggatcat ttttttctaa ataacggaaa
    11641 aaagcgcgac tgtcatggtt ccctttgatc aagtggattg ttccttgcag ttgagtgacc
    11701 agttccaaaa tttctgcatt tgctttttca tattgcggat gcatagcaag gtcgcctaga
    11761 tgataaacat ggtcggtttc ttttattact gcattccagc tagtgatcaa ctgttggttc
    11821 atttcatcca catttgcaaa gagccgcgga gcaaagtcgt tttttcctaa taagcgttca
    11881 tggaaaaagt gcatatcact gataaagtaa ttcatgtatc ttttgcctcc cttttttctt
    11941 agagtaaaca aaatcactta tctgagcaac tcataacatc taaaaagaaa aggttgtaaa
    12001 tgagacgtgc agctttaagc gataagaagc aattttgaaa aatagttttt cacaatttat
    12061 aaaattattc cttattgccg aaaggctggc cctttttatg agtatgccaa ttttttatat
    12121 cgaatgctca aaagatgagt gattttttgg ttgactatca tgtttgtgta ctgaataggt
    12181 ccagtagaac agtattttta cgacttgata attaagggat gaaattttca gaaatatgac
    12241 gtataatgta aaatactgaa aagttgcgac cttcaactcg tagatgtttc agaatctaat
    12301 tgaaatgatg gtgatatcat gacagacaac agcaaaacac gcgttgtcgt agggatgagc
    12361 ggcggtgtcg actcatctgt aacagcactt ttgttaaagg aacaaggata cgacgtgatc
    12421 ggaatattca tgaaaaattg ggatgacacg gacgaaaacg gtgtatgcac ggccacggaa
    12481 gattataagg atgtagcgaa agtagctgct caaatcggga ttccttatta ctctgtgaat
    12541 tttgaaaaag aatattggga tcgtgtattt gaatatttcc tagccgaata ccgcgcagga
    12601 cgtacaccta acccagacgt gatgtgcaac aaagaaatca aattcaaagc attcttggat
    12661 tatgcaatgg atcttggcgc cgattatgta gctactggac attatgcgca agtcactcgc
    12721 gatgaaaacg gcgtggtcca catgcttcgt ggagtagaca ataataaaga tcaaacttac
    12781 ttccttagcc aattatcaca agaacagctt tcaaaaacga tgttccctct aggcggcatg
    12841 gaaaaatcag aagtacgggc aattgctgaa cgcgcaggcc ttgcgactgc taagaaaaaa
    12901 gattcaacgg gaatctgctt tattggtgaa aagaacttca aacaattttt gagcaactat
    12961 ctgccagcaa aaaaaggaaa tatggtcact cttgatggtg aagtcaaagg acaacatgct
    13021 ggcttgatat attatacgat tggtcaacgt caaggactag ggatcggtgg cggaggagat
    13081 tcgcaagaac cctggtttgt tgtaggaaaa gatctagcga caaacacatt atatgtgggg
    13141 caagggttcc atcatcctgc attgtatgcc acaagcttgg atgcaagtga aatccacttt
    13201 acgacgaatg aaccaatgcc aaaagaattc aagtgtacag cgaaattccg ttatcgtcag
    13261 caagacgttc ctgtgacagt tcgcttattg gatgataatc gtgcagaagt cgtattcgac
    13321 gaacctgtac gtgcaatcac tcctggacaa gcagttgtat tttatgatgg aatggaatgt
    13381 ctgggtggtg gtttgatcga ccacgcatat caagaaacaa aagttttaca atacgtttaa
    13441 gcgatattaa atgaatcaaa acagctatgg ctaacaaaag aaacaccttt tcttttggat
    13501 agcatagctg tttttttgca ataaaattaa tttttattca ttttatactt gaaatacttg
    13561 tgtaaaaagt tcataatatc caataatatg aataaagttg ctttttgtta agcaagagca
    13621 gttgcaaaat agaagggaat cttttgcaag tgatttgttc aattttataa gtgagggtga
    13681 tggaaatgga aaagaaatcg acgggtatta gcaaatgggg actggttgct ctggttgtca
    13741 gttcgtcaat cggaagcggt gtgtttggga tcacaagtga tctggctagt tctgcagctc
    13801 cagggccagc gatcctttca tggataatcg ttgggattgg tattttagct ttggtattat
    13861 cgctgaatca tttaggagaa aagcgtccag atctagatgg tggtattttc ggttacgcgg
    13921 aagcttcatt tggtaaatta ggtggcttca tcagtggttg gggttactgg ttgtctgcgt
    13981 ggctgggaaa tgttgctttt gcaacgatgc tgatgagcgc catgggagaa tttttcccga
    14041 tattcaaagg tggacaaaat gttccttcta ttttatttgc aagtattttt atttggggtc
    14101 tgactttatt agtaaacaat ggagttgaag gagcgagcgt catcaataca atcgtgacga
    14161 tctgtaaatt agtgccatta ttcctatttt taatatttgt atttatcgca tttaaagtga
    14221 atatattcac tgctgatttt tggggtaacg tatctgataa tctagtgaat ggaacagggc
    14281 agcaaggaac gatctggttg cagattaaag gttgtttgat ggttatgatg tgggtctttg
    14341 tagggatcga aggcgcaagt gttttagcca accgcgcgaa gaaacgttca gaagcccagc
    14401 aagcatcgat cattggtctg ctttgtttat tgtttatcta tatccttgct tcgatcttgc
    14461 catacggcgt cttatctcaa gaagagttag caactatttc acaacctgct atggcaaata
    14521 ttttaaaagg aatcgtggga aattggggag cagcgctgat caatatcggc ttgattattt
    14581 cgatcattgg ttcttggttg tcttggacga tgctcccagc agaaacaacg atgttgatgg
    14641 ctcgagacgg tctgttgccg aaaggttggg gaaaaacgaa taaaaaaaat gccccaactt
    14701 attcgttagt tgttactgca atcttgacta atctattctt attgactttc ttagttactg
    14761 attatgccta tcaatttgcc tattctttat gtacagcggc aattttgatt tgttatctac
    14821 ttgtcgggat ctatcaagtc cagttttctt taaaagaagg aatcaaaaaa caagtagtga
    14881 tcggtgggat tgctgtagtt tttgaattga tgggcatcat tttagcagga ttttcttatg
    14941 tccttttatg tagtattgcc tatcttcctg gtttttgctt ctactggatc gcttgtcgtg
    15001 aaaccaaaca tcaaattacg ggaaaagaaa aagtaatgat tgggctgatt tgtgtggccg
    15061 cactgctttc aatcgggttg cttgctgtag gctggatcaa gatataaggg ggaaagaaac
    15121 atgaaaatcg caaatttcgg tgtagaagaa tggctaaata aatgggaaaa gaaagcggta
    15181 tatgatattg cacaaagctc gatcgaagca ttgacattag acgaactcgt tggcttagat
    15241 gggactagcg taagtaaatt tttcgaaaaa cgcaaaaacg aaccattgga ctatggctgg
    15301 atcgaaggat cagatgtttt caaacaagaa gtcgcttctc tttatcaaac cgtcgatccg
    15361 gaaaacatct tacaaacgaa tggagcgact ggtgctaatc ttttagcttt atacgcgtta
    15421 gtggaagcag gagaccatgt tgtttcgatg cttcctactt atcagcaatt atatgacatc
    15481 cccaaatctt taggtgccgc tgttgacttt gttcatttga aggaagaaga agattggcag
    15541 tttgatttag aacagctaga agccttagtc aaacctgaaa caaaaatcat ctgtttgaat
    15601 agtgcaaaca atcctacagg cacattgcta gatcggaaaa ctttagaaaa aatcgcagaa
    15661 attgctcgta cagtagatgc atatgtattg atagatgaag tatatgcgcc tttaacggat
    15721 aaaggtgaat tcttgtcgat cgtagatgtc tatgaaaaag gtatagcaac caattcccta
    15781 tccaaaacct actccattcc aggtattcgt attggctgga cagctactgg acctgagttg
    15841 gcagaagttt ttcgaaagta tcgagactac acgatgattt gcggcggagt cctgtcagat
    15901 gatcttgctg tacatgcatt gaagaataaa gaaaaaatat tggcgcgaaa caaaaagatc
    15961 attacagaga accttgtgat tttaaaacaa tgggtagcca atgaaccgaa agttgaactt
    16021 gtcgctccca attacgtctc tacttctttc attaaattag ccatcgaaga agatgaccag
    16081 acattttgta tcaatttatt agaagaaaca ggagtactat tagtaccagg aagtgccttt
    16141 gatttaccga agcatgcccg actaggttac tgttgtaaaa aagaaacctt ggaaaaggga
    16201 ttattcctct tatctgagtt cttgaaaaaa caggcctaaa caaaaacact gcaaggacag
    16261 tctgtgacaa ctgttcaagc agtgtttttc atttacgctt attgtctatg agaaggagag
    16321 tgccctgaag ctatggagac tcctgaagct ttttcttctg cagtttcttg ctgatggatc
    16381 tcttgtgtat tgtgtcgtac aagcaagaag ttgaccaaaa caagaagaga agtcgaaaga
    16441 aagacgccac gataaccaaa tgcactggaa acactggagc cgatcatagg ccctactacg
    16501 ttaccagttg cttggaaaga ttggttataa ctgaagatcc gtccagcagc ttgatgaggc
    16561 gaatattttg tgatcaatgc ttggacagca gggagcagac aagcatcaga gataccaatc
    16621 aaaaaacgta aggcagctaa ttgccaaaca ttcgtgacga atgccattgg gatataaacc
    16681 agaatcgcaa aacccaaacc aatcgcaagt attctttcac tcccaatctt gtcacctaag
    16741 cgaccaaatc ttggagcggc tatcaaagtg gcaatcccag gtatcgaagc aatgattccg
    16801 ctgatcaacg taacattccc atgtccatgc agcaattggc gaatgtacag actgatgatt
    16861 ggactaatgg aattgttgga tgcttggatg atcatcgttg tgacgaacat accgatgacg
    16921 acgtgtggat atttcaagtc tttgaaaatc tgtttggcag aaaccatgtt ttcttttttc
    16981 actgggacaa atttttcatg gacgaaaaac agactcaaca agaagacgaa aaataagatg
    17041 atccctgtaa tgaaaaaggt agggcgatag ccaaacgctg aagcagagat cccgccaaga
    17101 agcggtccaa gtaatgttcc tgtaacagaa ccagttgcta aggttccaag aacttgacca
    17161 cttttttctt ttggcgtccc agttgcgaca agggcagtgg cattactgat ataaccagaa
    17221 aaaatccctt gaagcagacg caatgcgact aattgataca cactagtcac gcaacccatc
    17281 aaagaaatga cgattgccat tcctaatgaa gcacgcaaca gcatcaattt tcttcctttt
    17341 tggtcggcta attttcccca ccaaggagag ataagcgtag tcaccaagaa agtagatgaa
    17401 aaagtcaatc cgctccaaaa gttcaattgt gacgtactat aattacctaa cgtatctatg
    17461 taaagtgaca taaaaggcat gactaaacta aatccgatac cagccataaa tgttccaaac
    17521 cataagacaa acaagttttg ttcccaagtt tctcttttcc taaataattt ttcctttatt
    17581 cttgcagcca attagactcc ttctttctga aaattttcat ctacagctct ttattataaa
    17641 ctacaatatg tagtttatca atgaaagtct cttttttctc tttctttttc gtggtaaact
    17701 gaaagagtga aggatttatt ttatgagagg aaacatggaa atgaaaaaaa gaaatctgtc
    17761 tcaagagaaa atcatcgact gctttcgaga actagcagaa gagatggatg ttcagcaaat
    17821 aacctttcag catttagcca aacgcttgga tatcaaggct ccctcgctgt ataaccattt
    17881 caaaaatatt cgtgatgtca aaacagcgct aacagcgaaa cttctgcagg aacttaatga
    17941 ccagctgcga cgggctcttg tgggaaaaag tggtcaggaa gcactgcaga tctatgcaga
    18001 aacttatcga tcctttgctt tttcaaatca agcagtgtat gagttactga tcagtgtacc
    18061 gcataccaat gaagctgttt tattagatgg aatccatgaa acgaatcaga tcattttgca
    18121 gatatttgag gcttttcaat taacgaaaga agaaaaagtt catcgaagca gggaacttcg
    18181 tagtatgatc cacggttatc tttccttacg ttttttagga tactttacaa aagaaccaac
    18241 aatcgatcca gaagacagct atttttggat gatcaataac tttatcgcta ctttgcctta
    18301 aacaatcaga ttcatttgta acaattttaa gatgttcatt aaatgaccct tttataagag
    18361 aaaattcttg caactttctt gaaaagtgac taagatgagt ctagaaaagc tgaaagaagg
    18421 agcacgagta tgtatcaagt aattaccatg ttcggggaca atgagccttg gtggtttttt
    18481 gaagactggg aagaagatat tgaagaagaa gaaacattcg agtctttcga agaagcacga
    18541 caagcgtacg aaaaacaatg gaacgaaatc catcaaaatt atgaatatat caatgcaaaa
    18601 agtaactttc tcagcgcctt ttggaatgac ggagatgagc gctggtgtga agagtgtgac
    18661 gatgatttac agcaatacaa gggacttgcg ttgttgaaaa atcatcagcc catcacagta
    18721 gaaagcagaa aggaatttta tgaaacaact aattctagcg gaaaaaccaa gcgttgcgaa
    18781 agacctaagc aaggtgcttg gtgctaatca aaagcacaaa aattattatg aaggacccaa
    18841 ggtgatcgtt acttgggccc ttggtcattt attaggattg aaaatgccgg aagatctgaa
    18901 taaggaatgg cagagctggc aaatggacac gttgccaatg atccctaaaa aactaggaat
    18961 caaaccactt cctaaaacag gacatcaatt aaaagcaatc aagcagctgg ctcagcgaaa
    19021 agatgtctca gaagcagtca ttgcgacaga tgccggaaga gaaggggaac ttgttgctcg
    19081 ctggatctta gagtatgtgc atttcaataa accagtgaag cggttatgga tctcttctca
    19141 aacagataaa gcaatcaaaa ctggattcaa gcaactaaga cctgcgaaag aatatgatgc
    19201 attgtatgaa tctgctcttg cccgggcaaa agcggactgg ctggtaggat tgaatgtgac
    19261 cagagcctta actgtgaagt acgcggataa tttatcagca ggccgtgtgc agacaccgac
    19321 actagcatta gtgagagacc aagaaaagaa aatcgagcag ttccgtccac aaacgtattt
    19381 cacgatcacg ttgcaagctg gaaaagaaca agccaaaatg cttcaaaaaa atcaatttgc
    19441 tttaaaaaca caagaagaag cacaaaaact tgttcaattg atcagtacaa gcaaaggaac
    19501 tgtcacagaa gtccaagaaa aattaaaaac ggaatctgcg ccattgcctt atgatttaac
    19561 agagatccaa agagaagcca atcaacgcta tggctattct gcaaagaaaa cattaagtct
    19621 tgtacaaagt ttgtatgaga cccataagat cgttacttat ccgcgtacag acagcaaata
    19681 tctgacaaat gacatgaaaa gtacgatgaa agaacgattg caagcagtca gcgactttgc
    19741 tcctgaggtc aagcagtatt tgaaaaacgg agccatcgtc caacagacaa aagtattcca
    19801 agacagtaaa gtgaccgatc accatgcact tttgccaact gaaaacagag cacgttatga
    19861 aaaattaagt aatgaagagc gaaaaatcta tcaaatgatt gtctctcgtt ttttaggact
    19921 gtttgctcaa ccccataaag tcagccaaac aaaagttacg gttgaatttg gtaaagaacg
    19981 attcattttt cggcaaaatc gtgtcgtcca agcaggatgg aaaggcgaaa ctgaaactga
    20041 gacggaaaca gtcaaatggg aaaaaggaat gagaatcaat cccgatttcg ccatcaaaaa
    20101 agaactatct gcaccgccta aaccattgtc agaagcaagc ttgcttggtc aaatggaaaa
    20161 atacagtctg ggaaccccag ctacgcgtgc agagattatc gagaaactaa tcaaatctga
    20221 actgatggaa cgtacccctc aaggactgca agtatctcct aaaggtaagc aattattagt
    20281 attggtaaat ccctctttag taacaccaga tttgacagaa agctgggaaa aagaccttga
    20341 agctattgct gcaggaaaga aacaagcagg aaccttcttg aatggaatcg aaaaagaaac
    20401 aaaacgcttg gtgaatgaga tcaaatcaag caaacaagaa tatcaagatt tctcgattac
    20461 ccaaaagaaa tgcccagaat gtggggccaa tctgcgcgag aaaaacacac gtgacggcaa
    20521 aatctatgtc tgcaccaatt cagattgcaa ctaccgcaga agaaaagatc caaaagtctc
    20581 taatcatcga tgcccgcaat gtcaccggaa aatggaaatc atcgaaggta aaaacggtgc
    20641 ttatttccgt tgtaaatacg atggaacgac agaaaaattg cttgataaaa aagagcagaa
    20701 gaagaaaatg accaaacatg aagaacgacg cttgctgaaa aaatatgcac aagcagaaga
    20761 accagaagaa agcccattag cagcagcact aaaagcagca atgaagaagt aataagatat
    20821 gaaagaggtt gtgatagaag tggtcagctt caagaaataa gaaggaattt acgaaaattg
    20881 cttctcaaat ttttgtgaaa tttcggctta tttccgaaga agctac
//
LOCUS       DAZUMK010000040        20802 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000040 DAZUMK010000000
VERSION     DAZUMK010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.040"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            48..1946
                     /locus_tag="ACG808_001402"
     CDS             48..1946
                     /locus_tag="ACG808_001402"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFS5047033.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(2245..2964)
                     /locus_tag="ACG808_001403"
     CDS             complement(2245..2964)
                     /locus_tag="ACG808_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFS5047034.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     misc_feature    3154..3201
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            3301..3633
                     /locus_tag="ACG808_001404"
     CDS             3301..3633
                     /locus_tag="ACG808_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047035.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            3620..5623
                     /locus_tag="ACG808_001405"
     CDS             3620..5623
                     /locus_tag="ACG808_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFS5047036.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            5642..6112
                     /locus_tag="ACG808_001406"
     CDS             5642..6112
                     /locus_tag="ACG808_001406"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFS5047037.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            6133..6717
                     /locus_tag="ACG808_001407"
     CDS             6133..6717
                     /locus_tag="ACG808_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HFS5047038.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            6732..7718
                     /locus_tag="ACG808_001408"
     CDS             6732..7718
                     /locus_tag="ACG808_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFS5047039.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            7708..8019
                     /locus_tag="ACG808_001409"
     CDS             7708..8019
                     /locus_tag="ACG808_001409"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFS5047040.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            8041..9822
                     /locus_tag="ACG808_001410"
     CDS             8041..9822
                     /locus_tag="ACG808_001410"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFS5047041.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            9815..11191
                     /locus_tag="ACG808_001411"
     CDS             9815..11191
                     /locus_tag="ACG808_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFS5047042.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLLERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            11198..11833
                     /locus_tag="ACG808_001412"
     CDS             11198..11833
                     /locus_tag="ACG808_001412"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFS5047043.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            11863..13218
                     /locus_tag="ACG808_001413"
     CDS             11863..13218
                     /locus_tag="ACG808_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFS5047044.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(13410..14804)
                     /locus_tag="ACG808_001414"
     CDS             complement(13410..14804)
                     /locus_tag="ACG808_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFS5047045.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            14896..15867
                     /locus_tag="ACG808_001415"
     CDS             14896..15867
                     /locus_tag="ACG808_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFS5047046.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            15864..16646
                     /locus_tag="ACG808_001416"
     CDS             15864..16646
                     /locus_tag="ACG808_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFS5047047.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(16815..17186)
                     /locus_tag="ACG808_001417"
     CDS             complement(16815..17186)
                     /locus_tag="ACG808_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5047048.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            17320..17763
                     /locus_tag="ACG808_001418"
     CDS             17320..17763
                     /locus_tag="ACG808_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFS5047049.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(17806..18615)
                     /gene="yidA"
                     /locus_tag="ACG808_001419"
     CDS             complement(17806..18615)
                     /gene="yidA"
                     /locus_tag="ACG808_001419"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFS5047050.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(18634..20001)
                     /locus_tag="ACG808_001420"
     CDS             complement(18634..20001)
                     /locus_tag="ACG808_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS5047051.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(19985..>20802)
                     /locus_tag="ACG808_001421"
     CDS             complement(19985..>20802)
                     /locus_tag="ACG808_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFS5047052.1"
                     /translation="RLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYDCTVIL
                     GMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTKKITID
                     DAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNGISVMM
                     YLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFVKEVKE
                     AFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL"
BASE COUNT         6634 a         3641 c         4456 g         6071 t
ORIGIN      
        1 ataacaactt tataatgttg agtataccaa tttttggagg cgttttaatg gaccgctatt
       61 tagatttact ttcagagaag ttccctacaa cggaagatgt catcacggaa atcatcaatc
      121 tcgaagcaat tttgcagctt ccaaaaggaa cagagttatt tttgagcgat atacatggcg
      181 aatttcctgc ttttgatcat attctgcgta tcggttcagg aaatcttaaa gaaaagatca
      241 aagacctttt ctctgaacaa atgacggaaa cggagatcag ccaattcact atttttacgg
      301 cttatccaga atatgtgttg acgactgatt ggtacaaaaa acaagataaa tcccagctga
      361 tccatcgttt gatcgatctt ttacggttta cgacagtcaa atacactcga tcaaaagttc
      421 gaaaaggatt gccgaaagaa tatagctata tcattgagga actcttgtat atcgatgatc
      481 gtatcaacgg gaaaaaagac tatgtaaaaa aaatcattga acaacttgtc ttgatgaagg
      541 aagaagaaaa atttttaaca aaacttgcgc agacgatcca aaaatctgtc atcgatcatt
      601 tgcatatcgt gggtgatatt tttgatcgag gaactcaagc agccaaagta atggatcgaa
      661 tcatggattt ttcctctgta gatatccagt gggggaatca tgatattttg tggattggtg
      721 cgtattgcgg ctctgaggca tgtttattga cgcttttacg gattgccgca cgctacaact
      781 atttatttga attagagaaa gaatatggat taaatttacg tccattattt tcttttgccc
      841 atcaaaccta tcagaagaat ccagtattta cccctaaaaa caggaaagat ctatctgaaa
      901 gagagcaaga agaattagaa aagatccatc aagcactttc tatcctccaa ttcaaatcag
      961 aacatcagtt gttgaatcga cgacctgaat ttaaaatgga cgatcgtaaa ttgttggaaa
     1021 aaatcaacta tgaggaatca acgatcgttt tagaaggtca gctctatgag ttgaaaggga
     1081 cttgttttca aacgatccag ccagatgatc ctaaaaaatt cttggaagag gaacaaaaag
     1141 tgatcgatag tttgatgtat tcattccagc actcgttaag aatgcaaaaa cacatgacgt
     1201 ttttgatcga aaaaggggga atgtatctca cgaacaatca gcatgtactg ttccatggct
     1261 gtatacctgt agatgaaaag gggcagctct tagcatttga attagaccaa tcttatcgcg
     1321 gaaaagaact gctgggattt tttgaaaaac agataactga aagcgcaaaa gatttgactg
     1381 caaatgaaga tttggcgacc gatttgattt ggtatgcgtg gagcgggcca ttttcgcctt
     1441 tattcggcaa gagtaaaatg gcaacttttg agcgctattt tctcacagag cctcatacgc
     1501 acgtcgaaaa aagcaatccc tattatcact tgcgagatga agagtggttc agtgaaaaaa
     1561 ttttggcaga attcgatgca aaagaagaag gttcagcaat catcaatgga cacacgccag
     1621 taaaagtaaa aaaaggagag tcaccgatca aagcaaacgg aaaagcattt gttattgacg
     1681 gaggtctatc caaagcttat caaaaaacaa ccgggattgc tggctactca ttactatgca
     1741 attcttacgg tttccaaatc gtgacacatt atccgttctt gcctattgaa caacaatttg
     1801 caaaaaaatc tgatcaaacg aatgtaaaaa aagtgatcga gcagcagctc ccacgcaaac
     1861 ttaatcggaa tacgacaaaa ggaatggaat tacagcaaca gattactcaa ctccaaaggc
     1921 tgttggatta tcgaaaggac gattgagaaa aatgctagcc aacattgctc aatagactaa
     1981 aaagcgactg caacaaaagg cgtgtcgcag tctccttcgg aattagctca aaaaacggta
     2041 aaatattaga agctattttt ctgtttattt ctctaatact caggtcaggg cgctgatttc
     2101 acgacctctt ccttaaacgg tgttctcctg ccaactcctc ggtcacaagt cacaatatcc
     2161 aacaaacatg agaagctgtt ttttgaaatt gctccttgtg actcagagtt gaacggcagg
     2221 ctcacgacct aatcagaaaa acgattaaaa ttcattcaca aagaaatcga ataaacgctg
     2281 ttcagatgaa tctacacgat gagaaagttc cggatgccat tggacaccaa ggattttgtg
     2341 catattatct ttactttgga ctgcttcaat gagtccatcg ttggaaaagg cgatggcttt
     2401 taacaaagga gaaagatctt tgactgcctg atgatgatag gaattcacaa agtatgaatc
     2461 agggagaagg tcacttaata aactgtccga aacgattttg acttcgtgag ttgcaaactg
     2521 cggggcagtg ggctgttgtt cgtgttttac ggaccatttt ggatataatg aaagatcttg
     2581 ataaagtgtt ccctccaaca cgacattcaa tagttgaagt cctcggcaga ctgcaaaaat
     2641 cggttttctt tgtttcaatg cttccaagat caatgcctgt tcgaattggt ctcgttgaat
     2701 atttgtttcc gtcagttttg gatgagggtc ttccatgtaa agttgcggac taacatcctg
     2761 accgcctgca agaatgagtt tgtcgatttt ctcaattagt tgaggcgctg aatctggaga
     2821 catgactggc agaataagag gtaatccttg agcatctaaa actgcatcca caaatccctg
     2881 cggtgtatag cttacttgat ttccttggaa agtatctgtc gctcgaatca gttgattccc
     2941 ggcaatgccg atgattggtt tcatttttat tccccctaaa gtagactaat tgtaacaagg
     3001 atttaaaaaa atttaaacta tgtaagactt gcccctatac ttatctgttc ttgctgtgtt
     3061 actatctgta aagagaattt tcgtgtgaaa ttcatgacga tagttgaaaa tattgcttaa
     3121 tttgttataa taaaaagtaa gcagaaataa aaaggttggg cgtaagcttc aaggatctag
     3181 ttcttcaagg ggtgagaagg acattttatt gaaggggcct gcctatacta agttatcttt
     3241 agaggtacat actttcaatg aaaaaagaaa gcatgtacct cttttttaaa tcgaaaggaa
     3301 gtgaagcaag tgttagaagc tttggaacaa atccgaaaag cagaagaaga aaatgagaag
     3361 cgtgctcgaa ccctacggca gcagttgaag acctatgcag aagaaaaaga gcaagaattg
     3421 gagataatcc aaacgaacag tcggcaacag ctgaaacagt tggtagaaga aaaagaacaa
     3481 ttcgaaaagc aagaattaga aaaagaaaaa atgaaattga aagcagaagc agacaaggtg
     3541 caagcaactt tgaaagacca atatcaaaag catcagagtc aagcaatcgg tgcaatcatt
     3601 gaaagggtga aagaaactta tggccgtcac taagatggaa aaagtgaccc tcatttcaga
     3661 taaaagaaat caggagagga ttttacaagc agtacaagga atccaaaatg ttgaaatccg
     3721 ggatctgtat caaaatagaa caaacaacca gtgggtagaa aaatattttc cagataccac
     3781 gattattgat aaagaagcaa aattaagcgc tttaggcaat cggctgaaag acatccaaga
     3841 agctatccaa ttcatacaac accacggcga tagccgtcaa aagaaactcc atttgaaacg
     3901 tcaagaactt tccttacaag acttggaagc aaattattcc gaagaaaatt tcatgaataa
     3961 attagaagaa gtccttgatt taaaagaaaa gtgggaacag ctaactgaaa aacgtgatcg
     4021 tttagaagaa gaggaagaat ggcttcttca ttggcaacag ctggatattg ctccaagtat
     4081 gtatgcaagt aagacgacca tttttgagat ggggactgtg agtgcagcaa attttgaaag
     4141 tttcaaggaa gaactaagta agcttgatga cgtttatatg gaagaaatag attatacgcc
     4201 aaaacaagtt tatctttcgt atgttgcttt aaaaaaatca gttgctgcta tagaagaaat
     4261 tgctgggaaa tacgggttca ataaagaaac gtatccttat gaacaaaatc ctaaagaaaa
     4321 attgcaagaa accaaacagc aattatccat tgttcacgag cagcaaaaaa agataacgac
     4381 agcgttaggt cagtgcagcg gctatatcca agaatttgaa tgggtagaag aagtcacact
     4441 tgccgttgca gaacgggaaa aagtaaaaga acggtttatc catgccactt atctgatcgt
     4501 tttgcaaggt tgggtagatg cagaagaaaa acagaatttg atgcatgtgt taaacgaaac
     4561 tgtatcagaa gaagatatat acgttagttt cgaaacacca aactcagaag agattcagca
     4621 ggaagtccct actaaattga aaaaccatcc gcttatcgaa ccttttgaat tgctgactga
     4681 aatgtacagt ctgccaaaat atgaagaagt agatccaacg ccttggatga caccatttta
     4741 tcttgtcttc ttcgggatga tggtcgcaga tgttggttac ggattgctga tgctcattgg
     4801 gacaatacta gctcaaaaac tatttgtttt accccgcggg atgaaacgtt tcgtaaaatt
     4861 ctttgagatt ctctcggtac catcgatcat ctggggattg atctacagtt catttttcgg
     4921 acaggcatta ccaaaagaac tatttggtat ccgcttgcct ttcccaatcc tatctacgac
     4981 ggatgacgtc aatacgattt tgattttatc cgtgattttc ggtctgatac agatcttggt
     5041 tggattattc gtggcagcaa aggaaaatat caggaggaaa gattacttga gtgctgtcag
     5101 tgatggtttt gcctggcaag ggatcctgat tgggatcgtt attgcattga ttggtgtcat
     5161 gttgataaaa aatacagcat ttgtttatct tggcgcgggg ctatcgatca ttgctgcttt
     5221 atgtatctta atcgtgccga ttatccaatc gccgtctaaa gtcaagggtg ctgccaaagg
     5281 agcgtataat ctttacggat tgactggtta tatcggtgat ttagtcagtt atacacgttt
     5341 gatggcacta ggtatttcag gcggaagtat tggcgcggca ttcaatatgc tagttgcttt
     5401 catgccgcca gccgctcgat tcagtgtagg gatccttttg atcatcgctt tacacgcact
     5461 gaatatgttt ttgacactgt taagtgccta tgtccatggt gcacgattgc agtatgtcga
     5521 attttttgga aaattttata caggaggcgg acgtgccttt gacccgctga aaacggcgga
     5581 aaaatacgtc aacatcaatc ataagaaaaa aaacaataaa taattggagg atttttatac
     5641 aatgatggat tacttaatta gtcaaaatgg tggaatggta tttgcagtat tagcgatggc
     5701 aacagcgacg attttttcag ggattggttc agctaaaggt gttggaatga caggtgaggc
     5761 agcagcagcg ttaacaacaa gtcaacccga aaaattcgga caggctttga ttctgcaatt
     5821 gcttccaggt acgcaagggt tgtatggatt cgttatcgcc tttttgattt ttatcaactt
     5881 aggaaatgat atgtcagttg tacaaggatt gaactttcta ggcgcttctc tgccgattgc
     5941 ttttacaggt ttgttttcag gtatcgccca agggaaagtt gcagcagcag gtatccaaat
     6001 tttagcgaaa aaacctgaac atgctacaaa aggaatcatt tttgctgcga tggttgaaac
     6061 atatgcgatt ttaggtttcg ttatttcctt cctattagta ttgaatgcct aagcataaag
     6121 gaggaaaagc agatgaacgc cattgaaaaa atcatctctc agatgaatga agcagcagag
     6181 caagaaagag cggcgttaga gcaagaagag cgaatgaaga tcgatcaaaa ctttgaacaa
     6241 aaacgtacgc aaatcgaaac agaacatcaa aaacaaaaag aaaaacaaat cgaattactt
     6301 gaaaaaaaat accgtcagct taggaatcgc caacaagtag aagtacgaca agaaaatttg
     6361 aatgcgaagc aggaattttt acgacgattg tttgcagatg caatagctga aatggagaat
     6421 tgggatgaat ctgaacaaat ccagtttatc aaaaattcat tgtattcatt acctttgaca
     6481 ggcaaagtaa cattcattgc tggagaaaaa tcagcagctt atctttctca gacattgctg
     6541 gatgaatgga acaatgaact tccctttatg atggttttaa gtgatgaaac tgttgctgat
     6601 caagcgggtt ttctgataaa cgatcaagga gttcagtata attttctttt cagcagcctt
     6661 gtacaagata tccaaggaac gatgagtttt gagattgcca atcaattatt cgaataagga
     6721 gggatcagtg tatggaatac cacgaactaa atcccttgat tcgcggaaga gagcttgaac
     6781 ttttgtccaa tgagacattt gaacggatga ttcaaacaaa ttctgtcgaa gcacttggtg
     6841 aaatattgaa atccacgatt tacagtccat atatttatga cggatatgaa tatgacttcg
     6901 aacagaatct tttaaaggag cagagccgtc tatttcgctg gttaaaggaa tcagcaccgg
     6961 agcctgaaat cgtttggatc tatacgatgc gctatacttt ccataatctg aaggttttga
     7021 caaaagccga gatgacaaat caaaatctgg accatttgta tatcgacgat ggattttatt
     7081 cattagaaac cttgaaggat gccattcata cgcaagtttc aaatgaactc cctgaaagca
     7141 ttatggacag tatacgagaa gtacgtgatt attttgaaga gtctaccatt cttcagggaa
     7201 ttgacgtgat ttatgatcga cagtttttga cagagcagcg acggcttgcc gataagctag
     7261 gatatcaaga attgctgaca gaagtcattg cttttattga cttgaccaat atcacaacga
     7321 tggctcgagg aatattgcag aaaagaacat atgggttcat gacgacggta ctttccagct
     7381 cgggaagtat cgaaaaagaa gtatttttga actttgtaga taaagatatg gaagattaca
     7441 cgaattttct attagagaca gattatgctg attttctgcg ccctgtcatc cgtgagggaa
     7501 atcttgatct cgtaaagctt gaacagttga aagatgatta tctaagttca ttgtatcaag
     7561 aagcacaaac ccaggctttc ggaccactgc cactactagc ctttttgaac gcaaaagagg
     7621 tagaaagcaa aaaccttcgt ttactggtta tcggcaaaag aagccatttc gaaccagaaa
     7681 caatcagaaa gcgggtgaga caagtctatg acgcataaga tcggtgtagt tggcgacaaa
     7741 gattctgttt tgccattcaa attgtttggc tttgatgttc gttatggtac aaccaagcaa
     7801 accgttcgtc aggcaattga agaaatggca aagaatgagt atggcgtgat ttatgtcaca
     7861 gagcaatgtg cagcattgag cccagaaacg atcgaacgtt acaaggaaca aatgatacct
     7921 gctatcgtat tgattcccaa tcatcaagga tcattaggga tagggctgga agaaattcaa
     7981 aaaaacgtag aaaaagcagt aggtcaaaat atcttatagt atgaaaagga gatagaaatt
     8041 ttgcaaattg gaaaaatcat aaaagtctcc ggacctcttg taatggctga gaacatgtct
     8101 gatgccagta tccaagacat gtgtctagtg ggagatctag gagttatcgg agaaattatc
     8161 gagatgcgtg gcgatgtggc gtctattcaa gtatatgaag agacttctgg aattggtcca
     8221 ggcgaaccgg ttcgctctac tggtgaagct ttatctgttg aattaggacc agggatcatt
     8281 tcacaaatgt ttgacggaat ccaacggcca ttagatacct ttatggaaat cacacaaagt
     8341 aatttcttag ggcgcggagt tcaattgccc gcattggacc atgagaaaaa atggtggttc
     8401 gaaccaacag tagaagccgg agaaacggtg agtgctggag atgttatcgg gatcgtagaa
     8461 gaaacaaaag ttataaaaca taaaatcatg gtgccaaatg ggataaaagg aacgatcaaa
     8521 acaatcgaaa aaggttcatt cactattgat gaaacagtat gtgtcattga aaccgaaaat
     8581 ggtgacaaaa atttgacgat gcttcaaaaa tggccagtaa gacgtagtcg gccaatcaaa
     8641 gaaaaattaa acccggatgc acccatgatc actggacagc gagtgattga tacattcttc
     8701 ccagtgacaa aaggaggagc agcagctgta cctggtcctt ttggtgcagg gaaaacagtc
     8761 gttcagcatc aaattgcaaa atgggccgat gtggacatgg ttgtctatgt gggatgtggc
     8821 gagcgtggaa atgagatgac ggatgtttta aatgaattcc ctgaattgat tgaccctaat
     8881 acaggagaat cgttgatgga acgtacggta ttgattgcta atacatcaaa catgccagta
     8941 gcagctcgag aagcttctat ctatacggga atcacaattg cggaatattt ccgtgacatg
     9001 ggttatgacg ttgctatcat ggcagactcc acttcacgat gggcagaagc acttcgtgag
     9061 atgagcggac gtttggaaga aatgcctggt gatgaaggtt atccagctta tcttggttca
     9121 cggcttgcgg aatactatga acgatctgga agagtcattg cattaggatc agaaggtcgc
     9181 gaaggaagta taaccgcaat cagtgctgta tccccgtctg gaggggatat ttcggaacca
     9241 gtcacacaaa atacattgcg ggtcgtaaaa gtattctggg gtcttgattc cagtttagcc
     9301 caaaaaagac attttccttc gatcgattgg atacaaagct attctcttta ttcaacagaa
     9361 gtagggaagt atatggatca tatccttcaa gctgactggg catccatggt caccgagggc
     9421 atgcgtattt tgcaggaaga acagcagctg aatgaaatcg tgcgtttggt agggattgat
     9481 tccctttcag ataatgaccg tctgacatta gaagtggcaa aatccattcg tgaagattat
     9541 ttgcagcaaa atgcttttga tgatgtcgat acattcactt caagagagaa acaattcaaa
     9601 atgttgaaga tgatcttgac ctttggtgaa gaaggacgcc gtgcgttgtc gttaggtgct
     9661 tatttaagtg agatcatgga cggtaccgta tcgattcgtg aaaggatcgg acgaagcaaa
     9721 tatatcccgg aaacagaatt agaaaaacta gatcaaatta ttggtgaaat caagcaaacg
     9781 atgcaaacga tcgtttcaga aggaggcatg agcaatgatt aaagaatatc gtacgatcgg
     9841 tgaagtcgtc ggtccattga tggcggttga aaaagttgct ggtgtcaaat atgaagaatt
     9901 aatcgaagtc cgtatgcaaa atggcgagat tcgtcgtgga caagtattgg aagtccaaga
     9961 agataaagca atggtccaaa tattcgaagg aacaagtggt atcaatttac gtgactcttc
    10021 tgtacgattt ttaggccatc ctttagaact aggtgtatcg gaagacatga tcggtcgagt
    10081 attcgatggt ttgggacgac cgaaagataa cggaccagag atcctgcctg aaaagcattt
    10141 ggatattaac ggagaagtaa tcaatccagt agctcgggat tatccagatg agtttatcca
    10201 aacaggtatc tctgcaatcg atcatttgaa tacattagtt cgtgggcaaa aattaccagt
    10261 attttctggc tctggtcttc ctcataaaga attagctgct cagatcgcgc gacaagcaac
    10321 ggtactcgat tcatcagatg atttcgccgt agtctttgct gctattggaa tcacttttga
    10381 agaagctgaa tttttcatgg aagattttcg tcaaacgggc gcaatcgacc gttctgtcat
    10441 gtttatgaat ttagcaaacg accctgcaat cgaacgtatc gctactcctc gtatggcttt
    10501 gactgctgca gaatatcttg cttatgaaaa gggaatgcat gttttggtta tcatgacaga
    10561 catgaccaac tatgcagagg cattgcgaga gatttctgca gcacggcgag aagttccagg
    10621 aagaagagga tatcctgggt atctttacac gaacttagct actttattag aacgagcagg
    10681 aagaatccgc ggactaaaag gatcggttac tcaaattccg attctaacaa tgccagaaga
    10741 cgataaaact catccaatcc ctgacttgac tggttatatt acagaaggac agatcatctt
    10801 gactagagag ctttacaaga gtgggattca gccaccaatt gatgtactcc cgtctctttc
    10861 gcggttaaaa gataagggaa ctggacccgg aaagacaaga gaagaccatg cagctacgat
    10921 gaatcagctt tttgcagcct atgcacaagg aaaacaggca aaagaattag ccgtagtttt
    10981 aggtgaatct gctttatctg atgtagacaa gatctatgcg aagtttgctg agcggtttga
    11041 aaaagaatat gtcaatcaag gattctacac gaaccgttcg attactgaaa cacttgatct
    11101 agggtgggaa ctgctatcga tgttgccaag aaccgaatta aaacgaatca aagacgacat
    11161 gctggacaaa tatctgccag aagggaagtg agttctgatg gcacgattga atgtgaatcc
    11221 gacccgcatg gaattgactc gattgaaaaa acagttgaca actgccacaa gagggcataa
    11281 attattaaaa gataagcaag atgaactgat gcgtcaattc atcctgttga ttcgaaagaa
    11341 caaccagctg cgtcaagaaa tggaagagtc tatgacagaa gcaatgtctg attttgtatt
    11401 ggcaaatgct tcgatcacgg aacctttcat cgaagaatta tttgttttgc cggcggaaaa
    11461 cgttgatttg tctatcgtag acaaaaatat catgagtgta aaagtacctg tgatgaattt
    11521 tgaatatgat gaacagctga cagacacacc tttggaatac gggtatttga attccagtgc
    11581 cgaactggat cggtcaatcg atcggtttac taccctgctt ccaaaacttt tggaactgac
    11641 agaaatcgaa aaaacttgtc aactgatggc gagtgaaatc gaaaagacac gtcggcgagt
    11701 caatgcatta gaatatatga cgatccctca attagaagaa acgatctatt atattcgtat
    11761 gaagctggaa gaaaacgagc gcgcagaagt aacaaggttg atcaaagtaa aaaatatggg
    11821 taccactgac taagtggtat ctttttttaa aagaggaaaa cgatgattaa acaaagcaaa
    11881 caaataaaga aacgtttgcc ggctgttcag cttctcgcat taggattttt tctcctgatt
    11941 ttagttggcg gcggcctact taccctacca ttttttagta atagtgggca tgggacaaat
    12001 tttatcgatg cgcttttcac tgcaacttct gctgtctgtg taacgggatt gacaacattg
    12061 aatacagcag agcattggaa cagtgctggt caatttttga tcatgctttt gattgagatc
    12121 ggcggtcttg gttttatgat ggttccgatt ctatgctttg cattgataaa gaagaaagtc
    12181 agtctcagca cacgtattat attaaaagaa gcgctgaatc tagaagaagt atcaggtgtg
    12241 atgaagctca tgctttacat cctaaaattg gcctttgtta tccaattgat tggtacggtt
    12301 gctttatctt ttgtatttgt gcctgaattt ggctggttga aaggtatctg gtacagtttg
    12361 ttccatgcaa tctctagttt ttgtaatgca gggtttgatt tgttaggaga cagtttagcc
    12421 aatcaccagc aaaatgttta tttgatcatg gtggtatctg ctttgatcat ttctggtggg
    12481 ttaggcttca tcgtatggcg tgatttactg gaatatcatc agaaaaagaa gatgacgatt
    12541 cattcaaaaa tcgcattaac cgttacaggt acccttttat taggcggatt cgttgtgttc
    12601 tttttcacag agcgcaatgg ggcaacgcta gtggaaggga atatgtttca acgattggca
    12661 aacacctttt ttatgagtgt gacacctaga acggctggat attattcagt tgattacctc
    12721 catatgagcc atgcaggtct gattttaacg atgtttttga tgtacatcgg cggaacatct
    12781 gggtcgacgg ccggcggatt gaaaacaact acgttaggtg tgttgctgat ccagatgcgt
    12841 tcgatgttga aaggacgtac gagagcggaa gcatttggcc gtaccatccg acaaggcgca
    12901 gtgttgcggg cacttacttt attttttgtt acgttgacgt tgtgtgtagt cgccatcatg
    12961 attttatcgg tgactgaaac gatacctaaa acttctggca tcgaatatat tgcttttgag
    13021 gtcttctctg cgtttggtac agtaggtctt acgatgggat tgacgccaga cttgactgca
    13081 atagggaaat tgatcattat agcgcttatg tacattggac gagttggtgt attgactgtg
    13141 gtcttctctc tgatgacaaa agcacaaaga acacaatcaa aatacaaata tccagaagaa
    13201 agtgtcatga ttggataatt tcatcttctg acagaaacct agtgatttag taaaataaga
    13261 aaataaaata atttttcttg cttcttggat actttaagtg caaaatgttc gtttattttt
    13321 tgaggcacta cccgttgttg aacctcgggt agtgcctctt ttttattgaa aggaatcaat
    13381 aagatttgat tcctttattt atcaataggt tataattcat atttttttat tttttctttt
    13441 acgatactta gtccagaagc taaaatttca aggttattag aggataggga aattcgaacg
    13501 aatgcatctg gttgcgcacg tgtagtaaag cgtctggaat gataaagtcg gataccgtta
    13561 gcaagaaagt cattctcaat tatcttaggg tcaatctcag gatcaactgg aagaattcga
    13621 tagtaaggga cacgattcgt tggtttttct actttgaaaa gagaatcaaa aagctgattg
    13681 gctctttttg taagacttaa tttagtttct aagatcctat ttgcttcatt tgaatggagg
    13741 atttgagtga tgacttccac atctaatcca gaagttttca catttatatt aaaaattgct
    13801 tgttctatct tgctttggac atgtactgga actacaagat aagcaactcg caatccagaa
    13861 caaataaatt ttgtcattcc agctaagtag attgtttggt taggtaataa attttggaaa
    13921 ggaggaatga tcgtatgttg atagtaagtt gttaaaaatg aatggatatc atcttcaata
    13981 acccatagac cttctttttc aatcacttcg gctaattgct ctcgtctttt gtgagagatt
    14041 tgaaacccta taggattatt acaagaaggc ataagaaaga caccatgaat cttctttttt
    14101 ctacattcct gaatcaatat atcaggtcgc attccttgct cgtcaaaatc aattggaacg
    14161 atctctaaat gatataactg agccaattca atgaaattag cgtatgtata tcggtctgtt
    14221 gctattctat cacctggaga gaaaagtgca gaaagtgtga tagccaatcc attttgaaca
    14281 ccggaaacaa tagatagaga ttctaaggac ggaatgactc cttgagtttt taaccaattt
    14341 gctgcagtag tcaattgaaa atttgtgcct ttgggatctt ggtaattaag caaaccaaca
    14401 agatttgggt tattactagt tgattgaata aacgggacaa cgaatttatt acattcttca
    14461 aaagaactaa ccagaccaaa atcaatgata tgttcggtta cttcattggt agagatggtg
    14521 atggactcaa agacattggg agaaataaat gtgccacttc caatattcgt atacagcaaa
    14581 cctttctcaa ttccatattt ataagccttg ccaacggtag taaaatttaa atctaaataa
    14641 tcagctagct ctctttggga gggtaactga gtgtttttct gtaatttatt ctttaaaata
    14701 tcttgttcta attgttgaat caacgatcga taataaggac gcttcaattt ttgtttgtct
    14761 ggtcgccaag ttaacgggta attgtcataa gaattaatag gcattttctc cactccttaa
    14821 aattgtatac catacaattt taccattgat tgtattgatt ttagaagtta atcttttgtg
    14881 aggagggaat tgtctatgaa aattattaat tttgaggaag tagtagcttc ttttagtttt
    14941 gaagaagcga ttcaagtaat gaaaaaatgt ttttttgatt atgaaagtgg taaaatcagc
    15001 caaaatcctc gattggtgga aattttacct gacggaaaag gcaaaaatat gtttgcgatg
    15061 atgcctgctt atttggggga gaacagatat tttggcacaa aagtcattac cgcttttccg
    15121 gataatcatc agtatcattt accttctcat ttaggagaga ttttcttgtt tgatgctcaa
    15181 aacggactac ccaaagcatt gataaacgca aatgcagtca cctggattcg aacagctgct
    15241 gtatctgcgc tggcaacaga ttacttagca aatccgcatg cagaaacact tgctttaatt
    15301 ggagctggtc aacaagcatt gtcgcatttg actgcgattt ctaccattcg tcccatcact
    15361 acagtgtttg tttacgatat tttagaagaa agacgaaata cttttatcgc taaagctaaa
    15421 aaacagtatc ctaaaataac gtttataaat gcaatatcag tagaagatgc agtacgtcca
    15481 gcggaaatca tttgtacgtt gacctctagt aaagaggcat ttcttcaaaa agagtgggta
    15541 agggaagacg cacatatcaa tgctgtgggc acatttactc ctgatacaag agagataagt
    15601 acatcgttga tggaaacttc tcaaatctat gtagatgatt actcagctgc cttaacagaa
    15661 agcggggacc tgcttatagc catggcagaa ggagatttag aggaatcttg tattgttggt
    15721 acattaggcg aattagtttc tgagaaaaaa acattttata aaggagaatc gaggccgacg
    15781 attttcgacg cagttggtct agctattgaa gatctttgtt gtgcagaata tatttataac
    15841 aaattagaga aaggggcttt aaaatgaact tagattatta cgtagttgat gcatttgcag
    15901 atgaggtgtt caaaggaaat ccagcagcgg tttatgtgct agaagaatgg ctgccagaag
    15961 gtacgatgca aaaaattgcc attgaaaaca atttatctga aacagcattt acagtgaaga
    16021 aaaaccaaga atttgagctt agatggttta cgccagatcg agaaatcgat ctttgtggtc
    16081 acgcgacatt agcaacagca tttgttttat ttaactatta caaaatacct gatgaaacga
    16141 ttaaattttc tactcaaagc ggtaatctat ttgtgacaag aattaaagat tattattaca
    16201 tggattttcc tagtattatg ccgaaaaaag tgccgatatt gtctgaatat gaagaagcga
    16261 ttggggctaa gattaaagaa gcctacttag cacgtgattt atttttcgta ttagaagatg
    16321 aaaaaacggt ggctaaattg cagccagatt ttacagctat caaaaatttt gaattaggaa
    16381 taggagtcat cgtgacagca gaaagtgtgc aaaaagactt tgtttctaga accttctttc
    16441 caaaattaac aatcaatgaa gatccagtat gtggatcagc acattctaat cttattcctt
    16501 attgggcaga aagaatgaat aaaacaaaat tgtcagctta tcaaatgtca tcaaggggcg
    16561 gggatctaga atgtgaatta aaagaagatc gtgtgataat aggaggaaaa gcagttttgt
    16621 tttctaaagg tacctcgttt atttgatttc tttcaaatga attaaaaata aattggtgga
    16681 gagtggcaac cttatagcta caatggttgt taaaaaaaga cgcaaacttt ttcaatttaa
    16741 tgaaaagttt gcgtctcatt ttaattattg gtgtttgttt tttattcaat caagagtaaa
    16801 atgccccata ctaactatcc tcggatgact tcgattttat aaccatcagg gtcaatgaca
    16861 aagtagtaag aaggcgcagt gccaggtaat ccttttaagt cagtaatctc aagaccggca
    16921 gctttatgtt gctcgtgtaa tttttctaga tcatcagctg ctatagcgat atgaccatag
    16981 ccgtctccta gatcatacgc accatgatca tagttgtaag tcaactctaa ttcatagtca
    17041 tccccaggaa gtgtcaaata aacaagtgta aatttgtttt ctggaaaatc acggcggcgg
    17101 ctttcttcaa aaccaaatgc cttttgataa aattcgattg atgcttcaag atctttcact
    17161 cgaacacatg tgtgagccat tttcatagat tcatctctcc ttttcctttt actcaatcat
    17221 aacaaaaaga agtaaggaaa tagttgattt gtgcttgttc atgagaaatt tctcatgtaa
    17281 aatggataga aaataaaact gttaaggtgg aaactattca tggataaacc aatttttggt
    17341 gaaaaaaagc aatttgctga ttatcaaacc cgttatgcag catatattgt catagaaaga
    17401 gatcagcaga ttgcagtaat cgaggctcca aatggtgctt tttttcttcc tggcggggaa
    17461 attgaaggag aagagacgaa agaagaagcg attgtccgtg agctgataga agaaatggga
    17521 attgtggcag aaatttcttc ttatctggga gaagctgatg agtattttta ctcgaattat
    17581 cgtgcaactt attattataa tccaggatat ttctttgttg ctgattcttg gaagcgtata
    17641 ggagaaccaa ctgaaaagac caataaaatt tggtgggtga gccctcaaga agcgcttgtg
    17701 aaattgaaac gtggtagcca ccgatgggca gtcgagagat ggatcgaaaa aaataaaaac
    17761 tgaaagaaag cgagtagatc agctttctct cagttttttt atttattaca gtacatattt
    17821 tttgattgct tcagctacac cgtgctcgtc gtttgatgaa gtgatgacat ttgctgcttc
    17881 tttgacaaga gaaacggcat ttcccattgc tactcctaga ccagcatatt caatcatgga
    17941 taagtcgttc tcgttatctc cgatagccat gatttcatct tgatcaatac caaggtgctg
    18001 tgcgagattc gcaactgccg cacctttact tgcttcttta tttagtactt cgaagtaata
    18061 gggcgcactt ttgaccgttg tgtatttatc acggaaagtt tgcggtaaac gcgcaattgc
    18121 tgcatctaaa atttctggtt cgtcgatcat catcattttg acgatgttca tgtctggtgt
    18181 catttcttca ggtgttcgat attttagcgg catcttgact aaacttgctt cgtggatcgt
    18241 ataagcgctg atatctcgat taggcgtgta gatcgctttg tcatcgatcg aatggagatg
    18301 actgccgaca cggcgtgcca tcacttcgat ctctaggaaa tcttcatgtg tcagtccgtg
    18361 acgggcgatg attttacctg atttcgtttg ttgtacgagt gccccgttat aagtaataac
    18421 gtaatcatta tcttgaaaaa ggttcaactc gtttaactgt tcctggacac ctggtaaggg
    18481 acggccagtg cataaaacga tcgaaacacc ttttgcaaca gcagcggcaa tcgcttcttt
    18541 tacttctttt gttacttctc ttttctcatt taataaagta ccatcgatgt cgatagcgac
    18601 tagtttgata gacataattt cctccaatat attttaattt atttcaatca atgcgccatt
    18661 acggatatgg ctggcaaatt cttgataagt ttcgtcaaat aaatcataat gatcacgcaa
    18721 ggacggatca accatttctt ttggaaagaa aaatcgctca tctccttgtt cttgtcccgc
    18781 taatgcagca acaagctgac taacctgtga taattctatc aaagttccgt catttcgcaa
    18841 aatctcgatt tgtgtgcgat gtctgccttg ttctgggcga taaaaatcat aaggtagatc
    18901 gtagctagaa ttcacagcag tgtaataaac aggattatat ccgacctttt ctactaacaa
    18961 agtgagttct tggactaatt ttgaatcccg gttgccagaa aaagtagcag atttcagtgg
    19021 tttacgattc aaaaaacgtt tagctaaatc atttaaaata ggatcagcag attcagacca
    19081 ttgagtaaag taagtgccca agaccccatc atcaagtttg aggtattctt ctaaagtaaa
    19141 gcttccgtca agaaatggca gaagtaattg agaatggagt tcaaaactgc tggcatcttc
    19201 ttgatacaac tcgtgtgcgc gatgaagcag atgctccaaa attacttcca taccacggga
    19261 aacaggatgg aagtacactt gcacatacat ttgatatcgg ctaacgatat aatcttccac
    19321 tgcatgcatg ccactcatgg aaaaagcgat cccgccttca taaggacgaa tcacacgtaa
    19381 tattcgggta agatcaaatg ttccatattc tgttccagtg aaataagcat cccgaagcaa
    19441 atagtccatt cgatctgcat cgatctgact agaaatcatt tggacaactt gtgggttggg
    19501 gtaagttttt tggatcacac tagctacctt ttcaggaaaa ccttcttcta ctcggttaag
    19561 aatctgatac acttctgttt ctggagaagt gatgatctct acagtgatgg cttcatgatt
    19621 cgtatggaag atatgttcaa atgtatgaga gtaaggacca tgaccgacat catgcaacag
    19681 tgccgcacat aaagtcacca gccgttcctg atcatcccag cctccgttgc ctattttttc
    19741 ttttgaaaaa tttcgagaaa agatatcgca gattcgtctt gaaatctcat aaacgcctaa
    19801 tgaatgagta aaacggctat gttctgctcc gtgaaaagtg aaagaagaag tgcctaattg
    19861 tttaatgcgt cgtagacgtt ggacttcttt cgaattgatc aaatctaaga ttacttgatg
    19921 ctggacatgg atatagttat ggactggatc acgaaaaact ttttccatag gcagcatttg
    19981 atatttataa ggggttgtca taatgtagct cctttatgtt attacgttgt tccattctct
    20041 ggatttggtt gttccattgc tccagctgga tttctttttt tagccagtcg gtagaatcct
    20101 gtaaatcact tgcagaatac agcttttcaa aagcttttgc aaatgcttct ttgacttctt
    20161 ttacaaaaag gggttggtca agcagttctt caagtgttgc cattgtagca ggatccactg
    20221 caggatagcc atttgtacca aagtcttctt ttaaagcaga ttgataaaat cgcttcatga
    20281 cttgcccgcg tttttcctga ttaccgttga cacttaagta catcataaca gaaatcccat
    20341 ttttgatccg acgctgagcg ataccggcaa attttttgcc atggatgctt aaatcataag
    20401 ttccaggaca ataagaatgg gtgatttcga atgcttgtat cggtgccttt ggaaaagcgt
    20461 atttagtcaa taggagcatt tgctcgtaag cgtcatctat ggttattttt tttgtgtcac
    20521 tttgggggaa aatcaaagaa atattcaaaa ttccttcatc tgatacgatc cctaatccac
    20581 ctgaatttct gatcactgga gcgtaacctg catctttcag tgtttggatc ccgtcactta
    20641 gaaaaggagt tcgaacatcc ttcataccta agatgactgt gcagtcatat tgccagaaat
    20701 gtaaaaagat ttgctggtgt tctttagaat attccgttaa tatatctgtt aatgcaaaag
    20761 gggaaaagtc gtttttttga taaatgtgct gatctagtcg aa
//
LOCUS       DAZUMK010000041        20322 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000041 DAZUMK010000000
VERSION     DAZUMK010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.041"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(16..321)
                     /locus_tag="ACG808_001422"
     CDS             complement(16..321)
                     /locus_tag="ACG808_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFS5047053.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(346..777)
                     /gene="ruvX"
                     /locus_tag="ACG808_001423"
     CDS             complement(346..777)
                     /gene="ruvX"
                     /locus_tag="ACG808_001423"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFS5047054.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(774..1040)
                     /locus_tag="ACG808_001424"
     CDS             complement(774..1040)
                     /locus_tag="ACG808_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFS5047055.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(1295..2020)
                     /locus_tag="ACG808_001425"
     CDS             complement(1295..2020)
                     /locus_tag="ACG808_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFS5047056.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(2381..2451)
                     /locus_tag="ACG808_001426"
     tRNA            complement(2381..2451)
                     /locus_tag="ACG808_001426"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2417..2419),aa:Gly,seq:ccc)
     gene            2568..3320
                     /locus_tag="ACG808_001427"
     CDS             2568..3320
                     /locus_tag="ACG808_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFS5047057.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            3336..3815
                     /locus_tag="ACG808_001428"
     CDS             3336..3815
                     /locus_tag="ACG808_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFS5047058.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(4066..5646)
                     /locus_tag="ACG808_001429"
     CDS             complement(4066..5646)
                     /locus_tag="ACG808_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFS5047059.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(5639..6382)
                     /locus_tag="ACG808_001430"
     CDS             complement(5639..6382)
                     /locus_tag="ACG808_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5047060.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(6558..7334)
                     /locus_tag="ACG808_001431"
     CDS             complement(6558..7334)
                     /locus_tag="ACG808_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFS5047061.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(7582..8418)
                     /locus_tag="ACG808_001432"
     CDS             complement(7582..8418)
                     /locus_tag="ACG808_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFS5047062.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(8429..9358)
                     /locus_tag="ACG808_001433"
     CDS             complement(8429..9358)
                     /locus_tag="ACG808_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFS5047063.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            9550..9903
                     /locus_tag="ACG808_001434"
     CDS             9550..9903
                     /locus_tag="ACG808_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFS5047064.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(9977..10633)
                     /locus_tag="ACG808_001435"
     CDS             complement(9977..10633)
                     /locus_tag="ACG808_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFS5047065.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            10859..12793
                     /locus_tag="ACG808_001436"
     CDS             10859..12793
                     /locus_tag="ACG808_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS5047066.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(12879..14102)
                     /locus_tag="ACG808_001437"
     CDS             complement(12879..14102)
                     /locus_tag="ACG808_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS5047067.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(14096..15967)
                     /locus_tag="ACG808_001438"
     CDS             complement(14096..15967)
                     /locus_tag="ACG808_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFS5047068.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(15988..17106)
                     /locus_tag="ACG808_001439"
     CDS             complement(15988..17106)
                     /locus_tag="ACG808_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS5047069.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(17189..18022)
                     /locus_tag="ACG808_001440"
     CDS             complement(17189..18022)
                     /locus_tag="ACG808_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5047070.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(18015..18932)
                     /locus_tag="ACG808_001441"
     CDS             complement(18015..18932)
                     /locus_tag="ACG808_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5047071.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(18955..19134)
                     /locus_tag="ACG808_001442"
     CDS             complement(18955..19134)
                     /locus_tag="ACG808_001442"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047072.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            complement(19159..20283)
                     /locus_tag="ACG808_001443"
     CDS             complement(19159..20283)
                     /locus_tag="ACG808_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS5047073.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT         6269 a         4045 c         3386 g         6622 t
ORIGIN      
        1 tcctagcttt atagtctatt cttcttcttc cgccatgaat gtattgaata cttcttcaat
       61 catgtcccat tctgcgtctg tttcgatttg ttctaactcg ccttctgttc catcttcgtt
      121 ttcgatgtat gcataagctt gtaattctac atcttcatct tctgaaacac cagcaggata
      181 taaaagaaca tagttacgtc caaattcttc ttgtccgtca actgttagta ggatttcata
      241 taatgtttca tttccttggt catccaccaa tgtgatgtgt tcgtgtcctt catggtcgtg
      301 ggaatggtta tgttctgtca taagattacc tcttttcttt tattattaaa ataattgctt
      361 acctgagccg tctaaataat tttgtaagat catcacagct gctaatttat cgatgacttt
      421 ttttcgtttg gctcttgaag tatttgcctg ctctactagc atacgttctg cctgaacagt
      481 ggttaatcgt tcatcttgat aatcaactgg caaggagaat agttctttga tcttttctcc
      541 gtatgacatg gaagcttctg cccttggtcc gatggaattg ttcatatttt taggtaatcc
      601 aactacaaac cggtctactt catattgtgc tactaattcc ttcaaccgat caaagccaaa
      661 ctcttcttct gcttcattga tccgaatgat ctctactcct tgagctgtcc agccaaaggg
      721 gtcactgata gcaacaccta ctgtcttaga accgacatcc aagcccatta ctctcataaa
      781 ttcactccat gatttgagag gtagtccttg acaattactt ccatgatctc atcgcgttcg
      841 tgacggcgaa tcaaatttcg agcatcacga taacgaggaa tgtaggccgg gtctccagaa
      901 agcaagtagc cgacaatctg attgattgga ttgtagcctt tttcttccaa agcccgataa
      961 acaatcgcta atgtttcgct cacttctttt ttgcgactat cgtcaaaacc aaaacgtacg
     1021 gtttcatctg taaaacccat gattctacac ctctttcccg caaggtaatt ccagcttacc
     1081 ttctacaacc ttattttact cgaagaaagg gaaaactaca aacaaatatt ccaaacttcc
     1141 gaataaatta agaaacacta tttgtatgtt tttttccttt tttctgtagc tttatcaaaa
     1201 gtactctttt cataaagtat acgctatatt tctttgaatg tatgttttta cacaattttt
     1261 tcataatatt cataaaaaat tcgaatagct gctactaatt ttttgttttt gttgcttgtc
     1321 ctgtctcata attgatcgtg tagccatctt gaacattgta gataaagaca ttgaattcaa
     1381 tttttttgtc ttcaatgctt tctgcttcca attgcactcc cctggctacc agttcattcc
     1441 cctcaaaatg aggagtgaca cggtagcgta catgatgatt tgtttctttg atatatgccg
     1501 caatatcatt ttcgtgagca agcatgtatg gagcattcaa cgaacgtgtc cctgtcatca
     1561 agtttttaat gttgttattt tgaccagtca gctggaaacc aattaaatgg ctgcgattat
     1621 ataaccattg cccgtcactt aatttctttt gtttccaacc tgtcgggtca atatacaaag
     1681 gctctctctt ttcagtaggg aacaaatctt ttccaagcat agcattggct tgtcctactc
     1741 tgttcaattt atcaatattt gaatagcttt cccatgttcc tttggaaagg ctaagatcct
     1801 cttttgtaaa ttccggctga ttcccattga tttctatttc ttgatgtttt ccatcatatt
     1861 ctggcacatc cgctacttga gaagcttgtg gtgtggaatc aaaaatagtt gtaatctgct
     1921 gaaagatatc tgaatctttt tgtgtggtgg aaacaccgaa tgcccctgca atcacagcag
     1981 cgacgatccc cacgatcgcc ttggttgttt ttttgctcat ttctttactc ctttactaac
     2041 agctccctaa atcttaacat atgttcccct ataaataaat actagaaaga aatagaaaca
     2101 gcaaaaaata aaacctgttt taagatggct taaaaataca aaaaagagac ggattcctcg
     2161 tctctaatca gtcaccactc tatctgtaag gtatttatta ttattcaaaa attttcttca
     2221 tactcaagac aatacactga tttcacaacc gcttggtaaa cggtgcgcaa aagcttgcat
     2281 ctgcggtaat aagtccatcc aagttaaaaa cataagaagt tgtttttgct tggctgttct
     2341 tattacttgt tgcagagcag ctttttcaca acctatttta agcgggtgac gggaatcgaa
     2401 cccgcgacat cagcttggga agctgggatt ttaccactaa actacacccg caatacaaaa
     2461 acagtatacg cctcttttat tccttcctca agttttttta ttctttacgc ttctggtatg
     2521 ttttgtagta aaataaaact aatcaatcaa ttattggaga agatgctatg acgggaaaag
     2581 aattagatct gttgctggac acttgtttac tcgctgggaa aatcatgatg gaaagcaatg
     2641 cggagatgta tcgggtagaa gatacgatga gtcgaattgc tttagcttct ggaaatttcc
     2701 gtttggtcag ctatgtcact caaaccggtc tctttattgg tcttgatcgt acctcgacca
     2761 tccgaatgga acaaattacc aaccgttcga ttaatttaga aaaagtagta aaagtaaatg
     2821 atctttcacg taaatatgtt gcttcagagc taacattaga agaactttac tgcgaactgc
     2881 aagcaatcga ccgagaccgt tctttctttc ctgtctggtt gcaaatcgtt agcgcggcag
     2941 tgatcagtgg aacaattatg gtgttatttg gcggaaagat gaccgattta cctgtgactt
     3001 tagtcattgg aggattggga tacgcagctt atctatatag tttgaaattt tttagaatca
     3061 aatttttatc ggaattttta tcttctttat tgattggcat agccgctatc tacagcgtac
     3121 gttttggctt aggaagcaat caggatctaa tcattatcgg atgtgtgatg cctttagttc
     3181 ctggagttca aattacgaac gctatccgtg atttgctagc tggacattat gtctcaggag
     3241 tttctcgcgg aacagaagcg atgatgactg ctacgatgat cggttttgcg attgctttta
     3301 ttttccagct tttttattag gaaaggaaca caagtatggg gacgatattt attcagtttt
     3361 cttttagttt tttagcgact gcggcatttg ccgttattac aaatgtacct aggcgttcat
     3421 tgatttgttg tggtctttct ggtgcttttg gctggatgat ctactggatc tgtgttcaaa
     3481 taggcggatc tgctgctttt ggttcattgc ttggttcttt aggagtagca ttcatcagcg
     3541 atcttttctc taaacgtttg aaaatgcccg taacgatttt caatattccc gggatggttc
     3601 ctctagtccc tggtggttta gcttatcaag cggttcgaaa tctcgttact gaatcctatc
     3661 gagaagcggc tcattatgcg gtccaagcaa ttatgattgc tggagcaatc gctttaggtc
     3721 ttgtattatc agaagtcttt aaccataaca tccgtaattt caaagtaaag cgtgacgcaa
     3781 tcatgatgaa acgaaaaaaa cggcttttta aataaacgaa gtgtaacaaa gaaaaggttg
     3841 tgaaagagac gttcagcctt gagcgatgac aaaatatctc acaaacattg cgacaagtaa
     3901 ccgcaggagc aataagaagt aattatgagt attaaaaaaa gttttgtaaa aatagcttct
     3961 cacattttta cataaaattg acttaatact gagaaactga cttttgaaca ccctctatct
     4021 gtaaaaaagg ggctgcgaca tgatttttgt cacggcccct cttctttatt caaatttttt
     4081 ccaaaacgtt cgttgataat aaacgatcca tagagcggaa agtattagaa ctaaaccgat
     4141 cacaggaata ttcactaacc aaaatggata aaacaaagcc aacattccgt ataagacgat
     4201 aagaataata ctgatgatca atgaacccat catagttgcg actaaaccca aacttcctga
     4261 acctctgtta aataattggt taatattggt ccagttcagc agcagcaacc gataatctct
     4321 agcgaaataa cgtagtgaca ataagtagca gccgaataaa gaaccaaata gcaagctagg
     4381 gataaaaaac aaaggcagct gaaaagacag tccgcttaat aatgcgatcc caccagttaa
     4441 aaaactctgc aatatccatc cgaatctgaa cttttctttt aaatattggt tcatagagat
     4501 tggcaaagaa cggataaaca gaaaattttc ttgatccaaa gaaataagat tgctgataaa
     4561 agaagtctga ttaactgtta agaaagccaa tgcgatccct accaggaaaa caacaccgat
     4621 taacttatta tctaaatcag ttaattttat ttcgcctcct agggcaaaag caagaataaa
     4681 aatgattgga gttaacaagg aagaagaaag aacttgcata atcaaattag gttcttttac
     4741 taattgagca ttgtaactaa ataaaagctg atgcacattt tggttctttt tatgggttct
     4801 ttttattttg aactcactgt ttgaatcagt cagttgctct gtaagctttg gtactatcag
     4861 ctgttttaca gcaaggaaaa atcccagagc cgtgatagct agtattcccc aacccaataa
     4921 accttgttga gaaagtggct gactggcagc atcatagaat ggcaataata tcgaaatcgg
     4981 atgccgatct gttaactcat tttcaatagt ccccgtttga tgattcatcc acatgattcc
     5041 agcaaatgcg atcagcatcg agctgccaag caagatacta gaaactgttt ttctatgttt
     5101 tttaaacact gctgttcgag ccaaaccaaa aacaatcaaa ctagccagac aaaaaagaat
     5161 aatcaaaaat agagaaaaca aaaggattga gataatgata gtaagcaaaa tccctagatc
     5221 agctcgccat ccggttaaaa taaacatgac tagaagcgga aaaacaaaag gaatgatggt
     5281 caaagcaact accaaaatct ttgctgaaaa aacctgttct tgtccaaaag gcagaggcaa
     5341 aaatacttgc aggtcatttc cttcaaaaaa gatattataa atgactgata tactttgagt
     5401 taaacctaac ataccaaaaa gtgccacgta ataagtaaag aaacctggca atttaacaaa
     5461 gttcatagca aacatagcaa atccgtaaat cagtaaaaaa atcagccctg aaaaaagatt
     5521 ttgagacaaa agatagatag ttaatcgctt tccctttttt cctttctctc ttgctttatt
     5581 cgtgagctgt ggattaacat ataaaagatt aactcgaatt aattctaata attgtcgctt
     5641 atacatacgg tccatctcct gtcgcttgcc ttccagccat tttcaaataa atcttttcta
     5701 gcgtttcatc aggtgagcca ttcaataatt cactaactgc cccctgataa atcaattcac
     5761 ctttcttcaa aatagccagt ttatcgcata actgttgtgc ggtatccaat acatgtgtag
     5821 aaaaaatgac tgtttttcct tttgcggcat gagccttcat catttctttc aaatcaaatg
     5881 ctgcttgagg atcaagtcct tgcaaaggtt catccaatac ccaaatatct ggatctggca
     5941 gcaacgtgcc gatcaaaatc gttttttgac gcattccatg tgaaaaactt gcgattgttt
     6001 cattttgatg actatacata tcaaaaagcg ctgtcaattc tgctaaacgc ttttcttttt
     6061 tttgtgtgtc taattcatag gcagctgaaa tcaagtccca atattctcca gccgtcaatt
     6121 gtaaaaaaat atctggggaa tcaggcacat aaccgatttt ttgtttgata ctcaatcgat
     6181 tttccgtcaa tttcattcca tctactaaaa tcgttcccga tgatggttca ataatactaa
     6241 ctaaactttt aatcgtagtt gattttccgg caccattatg tcctaaaaat ccaaaaattt
     6301 ccccttgtct tatcgagaga gacaagtctt ttaatgcttg ttttttcccg taacttttac
     6361 ttacattgat gaattcaatc atttctgtac tccttttgtt tgttattact ttttattgta
     6421 cccttcgtta ttggtggagt aaatgtcaaa aagagtgcat aacaaaaaag gatagaaacg
     6481 actaggctgc tacttgggtt gttctcttct acaggaaaac actcttctaa caggtaagat
     6541 atgagtcttt gttttcttta aaatgattca ttttctatct tttcttgcat aaaattcttt
     6601 agtttatcaa tacttctctt tcccatctct agttgttctc catttgcaaa tgttactatt
     6661 tcgtttactc tgctaataga tgtaatgttt tcagtattaa tgatataagc ttttaaatct
     6721 ttacataaat actcatattc gagtgatttt ttatattttt taattgtgcc gtttacgaga
     6781 atctcttcat ctttcaatac taggcctatt ttatttttat aacctggcat gctcattaga
     6841 taaaggattt catcgtaatg gatatagtga tcgctattac cataacggac agttgtccac
     6901 ctttttttcg tagcatgttg aacaggatag ttttcttttt ctttcaataa gtaactttgg
     6961 atgctttttt tatttttttc ataagtgaat tcttttatca aataaccact aggcgtaatc
     7021 tttcgattaa taacagttat tgctttattc ggatgagacg ttagataaac aattttacac
     7081 ttttcattct tttctctaag catttcgcct agttcgatac cgctataata ggcgttcaaa
     7141 tcgatgtcta ggaaaaaaat atctgaggaa tagatttcat tatttttaat gaattcaaca
     7201 aattgtataa cgttcaatcc ctgaacgatc tcaaatttcc aaggacttaa taaaattctg
     7261 gattcttcca aaatttctac tagcattttt tcaaaaacca aactatcatc caaaataaat
     7321 atacgcgtta ccataaaaag tacctccact cgatatttta ctatcaaaat catttcatac
     7381 aacttttcaa catacatgtt aggaataaat tgtaattctc ttcacaatct tttccaacac
     7441 ctttgaaacc gatatcaaat actttcagga tgctgcttct aaaataaata tcaaacaaat
     7501 gaatacatca tgcaaaaaaa gccagacaac cgacgtcctt gcgtgtcagc tgtctgagct
     7561 atgtttattt attttgttct tttaaaaaga acgatacaca tactgatttt tcggagcaga
     7621 gactttctca gcgtttgtaa caactacaga tgggatttca cgcttgaatg gttgataata
     7681 gtctgattgg atggttcctt ctattttata ccattcatca tttttaaccg ttactccttc
     7741 cggcatatgc gtcaacaaac caaagactcc tgaatctgcc acacaatgga taatgccgaa
     7801 tcggaacaag aagatatctg tattgctatc gttcggtgtt tggtaaacaa atccttcata
     7861 gctgattttt ttgccaatga attcgctagg gtaattataa attagctcca taacttctaa
     7921 atagttttca tctgtgatct tgatagtttg cttatcccca tacttttctt tcaactcatt
     7981 catttggtct aagtagtctg atttgttgaa ataaatactg gtatccggct ttaagtattg
     8041 tgtctgcatc tccggatccc ctactgattc tttacttact gggaaattaa atcctttggc
     8101 ttctacgatc gtagtgtcga gactaactgt tggaaacaac gtccccacga ttactggcaa
     8161 agctagcagt acataagcga ttccacgttg ataaggtttt gacaacccat ggtcatgatc
     8221 gtgctcgtgt gccttttctt ctgcttttcc gtttttgacc cacaaataca gctgaactaa
     8281 agccaaaaga gcagataaaa tcattgaaag aatagctaag tagcgataat ggacattgat
     8341 atattgatcg agtttgccag aaacttgcaa gtacatcatc aattcgaaat acccagataa
     8401 aatcaaaaac cgcagcatat tttccctcct aaataaataa tgtgaatcca ataacgctaa
     8461 gggcaatcag tccgatcagc gataaaataa agcgtcctgt aaaatatcgt ttcatcatca
     8521 gtagattttt gatatcgacc attgggccaa agaccaaaaa ggcagctaca ggtgctgtgc
     8581 cgaacagact taacaaagaa gatcctataa acgcatctgc ttctgagcaa agagacatcg
     8641 taaaggccaa taacagcatg atgataatgg ctaaaatctt tgttgagccg agcgttagca
     8701 taattctagt tggtaaataa gtctgcatcg cagctgcgat taatccgcca atgatcaaat
     8761 aacgacctgt atcaaaaaat tcgtcgattg aatgtgtcaa cacatgccca gtttgatgga
     8821 tcagctgatt ttttggttca ttcttctcac tttgttcttc ttcatggaca tgatgatggt
     8881 gattttctgc tactttagac gtgatttttt ctttcaatac agcgtctttt tgaaaatagg
     8941 caagccagac accaatcaca agggcaacaa aaaagctgcc tatcacccgc cagcttgcca
     9001 tctgccaaga attgccaaat gcgataaaag tggaaaaaat cacgattgga ttgatgatcg
     9061 gagcagtcag cataaaagca aacgctgtgt gaactggtac gcctttttta acaaattgat
     9121 gaacgatcgg tacgatgccg cattcacatg aaggaaagaa aaatcctaag atcgaaccag
     9181 taaaaataga gaagatacga ttttcaggaa gccatctctt cacacgttct ggggtcaaaa
     9241 atacttgtaa ggcgcctgag ataagacagc ctaataatac gaaaggtaat gcttcaataa
     9301 cgatcgataa gaaaatcgta ctcatctgca aaaccgaatt tggaataaaa gaaaacatga
     9361 gtttcctcct ttttatttct agttcattcg ctttgcaaat atacgctttt attcaaaaaa
     9421 aacaagttgc tctgtaaatt tttacacaac tcttattttt taccaaagca cttcaattga
     9481 ctttttctgt tattcccatt aaattaaggt ctcatacata tcacttctat cataaaaggg
     9541 agaataaaaa tgaaacaaac cactaacttg actgaagttc aagacatact gcaacagtca
     9601 tccgttgccg tcatttatct ttcgatgcct aattgcagtg tttgtcacgc tgttcgtcca
     9661 cgactagagg aacttctcac acattatgat attccggcac ttcatttgga tgcatttgaa
     9721 acaccagaag tagctagtaa atttgaagtg ctgactgcgc cagttgtcct gatttttcat
     9781 caaggaaaag aagtctttcg gcaagcacgg ttcatcgact ttaaaaaaat ccgttatcta
     9841 ctagatgaac tgacagcaga agatgattcc ctttcttatg aagaaatctt ccgaacaaaa
     9901 taatcgttgt ccaaacagaa aaaagcgatt gaacaagtga aagttttgcc ttcatttgtt
     9961 caatcgcttt ttatttttat tctgctgtat tttcggaatg cgcatttgcg tcagaatgca
    10021 agaaatagat caaggtttgc aattcattcg tcaaatcaac attttggatc aatacatctg
    10081 acggagcatt caatcgagca gcggtaaagt tcaagattcc tttgattcct gcttccgtta
    10141 actgattgac tacttcttgt gctctttcag cgggtatcgt taaaattgct acttcgattt
    10201 gttggatctt gatttgttct gacatatctt ccattggata aacggggata ccgtctacga
    10261 tgcgcccaac gatttcctca ttcacatcaa aagcacaact gatacgtatg ctattacttt
    10321 gatggaattt gtattttagt aaagcacttc ccaagttccc tacaccgatc agcgcaacat
    10381 ttgtcaattg atcgtcgttt aatgtctttg caaaaaagtg cataagatct tctacatcat
    10441 aaccataccc acgttttcct aattcgccaa aataagaaaa atctcgacgg atcgttgcac
    10501 tatctacttg aacggcttca cttaattcag ttgaagacac ttttttcttt ccagcattat
    10561 ttaaaatcct taaatagcga taatacaaag gcagtctttt agctgtcgcc tttggaataa
    10621 tatgatcttt cacgaaatga cctccacgta ttgtctaatt acatctttga tttctccatc
    10681 atagcagtat ttataggaaa aaatcaattc cattgctcag atattgtgaa attttattct
    10741 ttattcattt atttatcatt tctgcaaatc tcgcagtata atggaattta tggtagacta
    10801 ctaattgaaa aatagttttt tatttgagga gaattcacaa agtagaaaag aggattccat
    10861 gatattatta caagccaatc agattgctcg ccattttggt gcagaagtct tatttgaaaa
    10921 catacattta gaaatcagta caggcgctcg tgtcgcttta gttggcagaa atggcgctgg
    10981 aaaatcgact ttattaaaga tcattgctgg aattgaagca cctgatgaag gaaagatcgc
    11041 taaaaataaa acagcgactc tcggatacct cgctcaggac acaggacttg attccaatga
    11101 aacagtttgg aacgagatgc tgaaagcttt tgaacaagtt agacaaatgg aagagcggat
    11161 gcgcgagcta gagattttca ttagcgaagg tacgccagac acacctgctt atagttctta
    11221 tttaaaagaa tacgaccggc tgcaacacga tttttcagat cgaaacggct atggttatga
    11281 aaatgaaatt cgttccgtat tgcatgggtt taaattcgat tcttcatttt atgatcagaa
    11341 aatcgatact ttatcaggcg gacagcgcac acgtttagct ttagctcgca tgcttttgca
    11401 acaccctgac attttgattc tagatgaacc aacgaaccat ttagatatcg agacactttc
    11461 ctggcttgag aattatctgc aaagctatac tggtgcatta ttgattgttt cccatgaccg
    11521 ttatttttta gataaagtag tcaatgaagt ctatgaatta agcagaagaa aaatgcgcca
    11581 ttacaaaggt aactattcga aatatctaga tttgaaagca gaacagttag caagtgaatg
    11641 gaaggcttat gaaaagcagc aaacagaaat ccataaatta gaagacttcg ttgctcggaa
    11701 ccttgttcgc gcctctacca caaaacgagc gcaaagccgc agaaagacat tagaaaaaat
    11761 ggaccggctg gatcgtcctc aaggggatga aaaacaagca aactttctat tcagtataga
    11821 aaagccttct ggaaatgtag tcttgcaaat cgaagatgcc gctatcggat atgataatgt
    11881 caccttatct gaaccaatca atctagatat ccgtcgtcaa gaagcgattg ctctagtagg
    11941 tccgaatggt atcgggaaat cgacgttgct gaaatcaatc attcaccaga tcccttttat
    12001 ccgtggaaaa gaaacgctag ggacaaatgt caccatcggc tattacgatc aaggtcaagc
    12061 tgatctgcat acaaacaaga cgatcctagc agaattgtgg gatgagcatc ctaccactcc
    12121 agaaaaagag atccgaagtg ctttaggtgc tttcttattt tctggcgaag atgtagaaaa
    12181 accaatccct ctgctaagtg gcggtgaaaa agcacgggtt gctttagcca agttagcgat
    12241 gaacaaagaa aattttttga ttttggatga accaacaaac catttagata ttgacaacaa
    12301 agaagtatta gaaaatgcat tgatcgatta tgacggcaca ctgctttttg tttcccatga
    12361 ccgatatttc atcaatcgga tcgcgacaaa agtcgtcgaa ctctctgaaa atggcagcaa
    12421 agtctatcta ggagactatg attattattt agagaaaaag caggaagaag aagaaatcgc
    12481 cgaattgttg gcacaagatg agccagaatc tgctcctgct cccacgaaca aatctgatta
    12541 ttaccaaagc aaagaacagc aaaagcttat ccgtagcctt caacgaaaaa ttactcaggt
    12601 tgaagaagaa atggcgcgga tcgatgaatg gatcgatcag ttggaacaag aaatggtcgc
    12661 gccagaaaac ctggaagatc acgtaaaact caatgagctg aaccaagatt tagaagctgc
    12721 tagacaagat caagaaacaa aattgacgga atgggaagaa ctaagcttgg aattagaaga
    12781 atatgaaaat taacgaaaaa accaaaacag aaaccattca tgctgttttg gtttttcttt
    12841 ttttatttta tcaaacaaaa attccatatt tacctttttc accaataaga agaccaatta
    12901 ctagtcattc tcactaaagc ttctaaataa tagtaatcac cccaaataca aggtgtattt
    12961 accccattat ttttgggctt attataaaca gactgtgtta aaaagccatc ttcttcttcc
    13021 gttgtagcat atctttcttt caattgtcgc ataattttcc aaacagcaga ctggtataag
    13081 ttcttttctg ttttgtctaa tagacctgat tctattaatt ctaataatcc acaaaccatg
    13141 atcaccatac tagatgtatc tttttcttct ctgctagaat catcaaagat caaatcccaa
    13201 taacaaatgg tatcttctgg caaccgattc ataaaataat tggctaatat tttgcttaaa
    13261 tcaaaatatt tttgttcttt caaataacga taacttaatg caaaaccgta aatcccccat
    13321 gcttggcctc tagcccaaca ggaattatca ctataacctt gatgcgtact tccatatctt
    13381 ggttttccat ctttgacatc catataaaat gtatgataag ttgaaccatc ttctcttact
    13441 aaataatctg ctgcattttg aatatgagcc tgagcagctt ttttatattt ttgatttcca
    13501 gtttcttcac tcgcccaata caataatgga agattcatgc aacaatccat gatcattcta
    13561 ccttgttgtg aaggatcact aggatctccc caagcttgta taactcctgc cttctcccaa
    13621 aaacgctgaa gtaatgtttc tgcagcatca attgaagctt ccttcatttt tcgatttccc
    13681 gttattttat aatctgctac ggttgataaa gaatacaaaa aaccaagatc gtgatgatta
    13741 gtgataattt gacagtcaat cctgtggata aaatctatat ctgctttttc agctgtttgt
    13801 tgaaataaag gatcacccgt cttctcaaat aacaaccaat taattcctgt ccaaaatcct
    13861 tctgtccaac caatattgtc tcttaatgga taacgtcctt ttataggaca ctcgcttggg
    13921 aattgatact gactgttact gatacgtttg attaacctct ccgtgtgtct gcgacaacgc
    13981 tcgatctcat tattccattc tttttccatt aattctttat aacattcatt tatccatttt
    14041 tctggatgta ttatctcttc aaacatcact ttatcaagca tttccccacc agcctttagc
    14101 ataattttag tcttttttta ttgtttttat tttggatagt tagcccggtt gtcccatcaa
    14161 aatcaatttt gggacgattg ttccatactt ctaaactata atcccgatct tgatgagcaa
    14221 ccactcctgt tccgagccaa tgggttcctt ttccaaatgt accctctaat ccaggaataa
    14281 aagtgttttc ccatgtcatc aagtttgtat tgggcaaacc cataatagct gtggattttc
    14341 gtttaatagt atcgtcactt aaacagataa tacctgaaaa gccagcttca ctcaccctat
    14401 agttttcctt gtcttcttct atgattattc cttctttctc aggagaacat tcaatagcaa
    14461 accctgtctc atatagaaca tattctgtat ctaattctac tttgtgtacg cgaatctgcc
    14521 agccttcaaa aggaataagc caagttctga tttttgcctg taaagtaggt tcccatgtag
    14581 aggcaagtac ctctttctta catacaatgt ctcgtacatt atttcgaacg atgaactgtc
    14641 cataggtttt gatgctctca ttcttaaaac tggtcgtgat atatgtgtcc gctcgctgaa
    14701 tccctagcat tgaatccatt gccatttctg ctttcaattg attcgctcgc ggtatcgaaa
    14761 aaccaaattg actagaataa gcaaatttta aatatttttc attggtcaat gtatggaaat
    14821 ttggccctaa ttgtcccgca ttcaaaaaaa atgtatgtcc attatcatgt tgtatgagca
    14881 aattggcttc tgaaagaagt ttagttcctt cacgaatagg cataggcatt tctttagccg
    14941 tccaatactc atggtcttca ggtagcgcca acaataaaaa aatcttattc agccataatg
    15001 gagataacgt actattatag ggttcagtca atgtcaattg agggtaggta taaccaatat
    15061 tcaggatacc tttttcatca aatatttctt tttgcatcca ccatcttata ttccgattga
    15121 ctaacccctt gatttcacct aatttaaaag gcaaaagatc ggcccacaac atagctgacc
    15181 aaaagcttga aactgcaaaa cgataaatca tgcttcgacc aaaaggcaca tttgcaccat
    15241 cttccgcaaa aaaatgaata tagtctctag aaaaatcaat agctcggttc ttatatatct
    15301 ggctaatggg cttttctggt tctagtacgc tataaatcaa accataataa tgaaatgcaa
    15361 aaggattata ataatcttgg cgacctcttg aactgtctcg ataccagcca ttttgaagat
    15421 aacaattttc tatattatca aatgcttctt tcagtgctga ttctttaact ggataaccaa
    15481 atttttggat aattgttccg acaattatct taaaaaattg ccaattacca tctgcacatt
    15541 catattcaaa aatttgcgag aaccaatgaa taatctggct tttttctcct gtatctaatt
    15601 ttttccataa taattgttca tgatgatata acgctaataa cattgcgggc atttctacca
    15661 tacgctggtc tctttcatgt atttctcccc aataatcggg atgggaagga tctgttccag
    15721 cttttattcc tgatagaatt aatttcaacc atcgttcatc taattggtca attgcgggcc
    15781 ccgctcccca taaaacccga gagaatcctt ccattcctgc aattttattt ccatatccta
    15841 ccccttgatg tatataatca atttgtgcgc cacttgctcg ataaaacggc tggagtggtt
    15901 gatttatctt tttaaataag tcaaagtaat cctgtctggt ccttaatagt ggtactggat
    15961 gtttcattgt attttctcct ctttttacta ccaaatagct aattccgact gttctaatcg
    16021 aagtaagcct tcaataaaaa aataatcaga atagatcagt ggcacattat aagtcagttc
    16081 tctatggtat gcttctgctc cgcctccaat gatcccatct tcatccatat tccaatttgc
    16141 atagtgcttt tctgtctttt ggagtatatt gatagcagct tcatgataca tttttgatcg
    16201 tacagaggaa acatatcctg ctaattccag aagaccgcat gcagcgcata ctccagcact
    16261 ggtatcaata ttcgaatctt ttacagcaaa atcaatgaga ggaatataac cagttttttc
    16321 gattttctga ataaaattta ctgctatctt ttctgctata gctagatagt acggattttt
    16381 tgtgtaacga taacttaacg cgaatccata taaagcccat gcatttcctc tgctccaaca
    16441 agaattctca ttgtatcctt gcccgccaag taaccgaata tatttccctg attctgcatc
    16501 aaattctgcg atatgcccgg ttgatccatc tttcctcact agatacttag ctgttgtatc
    16561 tgcatgctgc cgagcaattt tagagaacct cggatcacca gtttccctcg aagcccaata
    16621 cagtaatggt aaattcatca tactatcaat aatcatccaa ccaggtttat ggtcattcca
    16681 tgctactaaa tagttccctg aaatattata gcgtccagca agtaccgtag ctgcatgtaa
    16741 ccctagttgt cgactatgtt tgttttgaga ctgttgataa tgtgcaatag ccgatggtaa
    16801 gaataaaaaa cctacatcat gatgaacgtc tgaaaaatgg tacagggcag attctaataa
    16861 ttgttcttgt tgtattgctg cctctttgaa cttttctcgt tttgtcttgt gaaagagctg
    16921 ccataaaata ccactccaaa aaccattggt ccaccagtcg attcctctgt cctttattac
    16981 gtctgtatat tttccgttgt aagaaatata tggaatttta ttccccattc tgtctaattc
    17041 cgcatctatc ttttcagaag ctacattcaa aatttttctt gaccattcct tcgtctgttc
    17101 attcatggat atcccgccat tcttttaatt tttatgaatg tggagttgct cctagaacga
    17161 actagatggt taacattcgt tatttttttt acttatttgc agctccaatg tcattctcct
    17221 taaatttaga ctgcgcccac acataaatca agaccattgg gatcactgaa gttgctaaaa
    17281 ttagcataat tgcgccataa ttaattggct cacctgtact agaaaccgtc atatttttga
    17341 taattaaggg aaccgtatat ttatctttat ctgaaagcat gactaatgga aatagatatt
    17401 gattccaact tcccataaag ataagtaaac cactagtaat aatcgtaggt gataaaatcg
    17461 gtacagcgat tttcgtaaaa atcacccact cattggcacc atcgattctg gcagcctcca
    17521 tcagtgctgt aggaaattga ctgctatatt gtcgcatcat aaacactgaa gttgtactgg
    17581 caatcgaagg gatgatcaca cctgcataag tattcgataa tcctaaacga ctcataatgt
    17641 aaaataaagg aatcaatgtg gtaaaaaaag gcaccatcct agatagcata actgtattga
    17701 acaacagttt attccccata aaacgatact ttgctaatgc ataacccgcc attgtagaac
    17761 tggctgtagc taaaactgta ctgattacag caataacaat cgaattccaa ataatcaagg
    17821 gataattata tctcgccatg atggcttgcc agttttttaa tagcatatcc ccaaacttga
    17881 aattgattcc gccacttaaa atttcttgat tactttgcgt tgaagctaca atcatatata
    17941 aatatggcat caaaaatatt aaggcaccaa acacaataat gacgtacgta aaaattctgc
    18001 tttgtatctt ttgtttagtc atttttatca cctccgaatt taaattgaat agccgataat
    18061 ataacagata caaacaccat cgtccaggca atagcagagg cttttccaaa atcaaccatt
    18121 tcaaaacttg ttttatataa attgactcct acgaccaaag ctgaattttc tggtccattg
    18181 gaagtcccaa agaatatatt tgggatattg aaattaccca agccattgat agtggctaaa
    18241 actgtggcca ttaaaatgat tggctttagc atggggatcg ttaataaaaa gaattgccga
    18301 aatttcgatg ctccatctat ttcagcactt tcgtatatct ctttagaaat cccttgaaga
    18361 ccagctaaaa ataaaatagt gttatatcct acactaagcc aaatcatcgt gatgatcatt
    18421 gatattcgta tccaaaaagg atttgttaac cactgtacac cgttaattcc tagcgcattc
    18481 aaaaatccat tgaataaccc agtcggattg aaaaaagtca tgaatacgga agctacagcc
    18541 accgttgatg taatggttgg aatataatag acagttctaa aaaatccttt tccttttaac
    18601 tgtgctgaat tcaataaaac agcaaagtaa atagctaata gcatcgttat tggaattgat
    18661 cctgccatga taatcaaaac attcgtgtaa gatttaataa acaatggatc tcggaagata
    18721 gctagataat tgtcaaatcc aacaaattca aatgagtagc caattttcat aaaactaaaa
    18781 gcaaacgaga taatgatggg aatcaaaatc gtcacaataa agaaaataac aaaaggtgtg
    18841 accaatccat aagcactttt tttatgagac gtatctgttg cttttttctt gctgttcttt
    18901 attgtcttgc ttggtaacga agattgcatc atcgttaagt cctcctaata ttttttaatc
    18961 accagttaat tttacgccgt acttatgagc aaaattttct gcttctttta gatatgaatt
    19021 ttcaaaatca cctgtttgcc aatagttata tgcagcggta gtcaaataag acgtagcatc
    19081 tgaataagtg gctggtaaaa cagtatttga tgtattttca ttgattttat tcaatagttc
    19141 atagtatttt tgtcctcctt agtagtcaaa ctttttctca gctgcttctg tttgataggc
    19201 caatgagtta gatgtagcca cgcccagctc cagtgcgttg gctaagcatt cttcatctgt
    19261 cagcataaat gtaagaaatt gttgtgccac ttgtggattt ttggcatctt tagggacata
    19321 aaaacttgat cctcctgata ctgcaccttt tcctggattt tcttttgaaa aaggaatcag
    19381 tggagccatg cgccatttgt cagatgcatc aggaaaattc aaagacatat tcgtagcata
    19441 ccagccgcca gcaacaaaca ttgcagattc ttgaaatgct ttctcagcat cttgtgcacc
    19501 gtagaaatgt acaatatctg agtcgatcat ttctttgatt aactttgcag catgtttcgc
    19561 ttctttagta ttcagacgga tatttccttc ttcatcaagc aaagattgat tctgctgggc
    19621 tagcatgttg ataaacaatt cttggtcccc agattctggc aaggcaattc caaaactacc
    19681 agttttttct tttaatgctt ttgccacctg gatatattct tcccaagaac tgatttcttc
    19741 aaaatctaca cctatttcag caaatttttt tggttgataa taacaaagtg ctggggtaaa
    19801 atcactagcc caaggaataa tcgtttcatt actcgtttgt tgacgcaaaa tattcaattt
    19861 ttgatcataa aactgatcgc catatttgtc ataaaatcca tattcatcag cactataaaa
    19921 agcatcttga aatttttcca gcacacctgc tgctgaagtg tctgccatgg agataatatc
    19981 cggcatatct tcgtgtgtta ccaattttgg ggtgatcatt tgtactgttg ctgttcccga
    20041 agctacattc acaattttta agtcaatatt tgctccagtt ttgcggttat agatttttgc
    20101 agcattttca acatagtttg tcagtttgcc gtttgcgaca tatacttcta atgtagtggc
    20161 tttttttatc attggatctg cgatgtttgc tgttacttct tcgttagttt ttccacatgc
    20221 acttaagctg aataataggg taatgtttag caaagctatc accaatttgt atcttttttt
    20281 catgtctatc ccttctttct tttcaagttc ttatataaaa cg
//
LOCUS       DAZUMK010000042        20242 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000042 DAZUMK010000000
VERSION     DAZUMK010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.042"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(66..623)
                     /gene="frr"
                     /locus_tag="ACG808_001444"
     CDS             complement(66..623)
                     /gene="frr"
                     /locus_tag="ACG808_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFS5047074.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(626..1348)
                     /gene="pyrH"
                     /locus_tag="ACG808_001445"
     CDS             complement(626..1348)
                     /gene="pyrH"
                     /locus_tag="ACG808_001445"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFS5047075.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1484..2365)
                     /gene="tsf"
                     /locus_tag="ACG808_001446"
     CDS             complement(1484..2365)
                     /gene="tsf"
                     /locus_tag="ACG808_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFS5047076.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2464..3246)
                     /gene="rpsB"
                     /locus_tag="ACG808_001447"
     CDS             complement(2464..3246)
                     /gene="rpsB"
                     /locus_tag="ACG808_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFS5047077.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3605..4084
                     /locus_tag="ACG808_001448"
     CDS             3605..4084
                     /locus_tag="ACG808_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFS5047078.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4301..5392
                     /gene="phnW"
                     /locus_tag="ACG808_001449"
     CDS             4301..5392
                     /gene="phnW"
                     /locus_tag="ACG808_001449"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFS5047079.1"
                     /translation="MYKLLTPGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSGSFGVESVLSSVIRPEDTVLICSNGAYGQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQEKDLL
                     FIVDAMSSFGGIPIRVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5385..6170
                     /gene="phnX"
                     /locus_tag="ACG808_001450"
     CDS             5385..6170
                     /gene="phnX"
                     /locus_tag="ACG808_001450"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050194 - phosphonoacetaldehyde hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFS5047080.1"
                     /translation="MIKTVIYDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(6517..8208)
                     /gene="argS"
                     /locus_tag="ACG808_001451"
     CDS             complement(6517..8208)
                     /gene="argS"
                     /locus_tag="ACG808_001451"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFS5047081.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8630..9577)
                     /gene="arcC"
                     /locus_tag="ACG808_001452"
     CDS             complement(8630..9577)
                     /gene="arcC"
                     /locus_tag="ACG808_001452"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFS5047082.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9692..10711)
                     /gene="argF"
                     /locus_tag="ACG808_001453"
     CDS             complement(9692..10711)
                     /gene="argF"
                     /locus_tag="ACG808_001453"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFS5047083.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10802..12031)
                     /gene="arcA"
                     /locus_tag="ACG808_001454"
     CDS             complement(10802..12031)
                     /gene="arcA"
                     /locus_tag="ACG808_001454"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFS5047084.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            12492..13193
                     /locus_tag="ACG808_001455"
     CDS             12492..13193
                     /locus_tag="ACG808_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS5047085.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(13765..14781)
                     /gene="tsaD"
                     /locus_tag="ACG808_001456"
     CDS             complement(13765..14781)
                     /gene="tsaD"
                     /locus_tag="ACG808_001456"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFS5047086.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(14778..15242)
                     /gene="rimI"
                     /locus_tag="ACG808_001457"
     CDS             complement(14778..15242)
                     /gene="rimI"
                     /locus_tag="ACG808_001457"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFS5047087.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(15249..15791)
                     /gene="rimI"
                     /locus_tag="ACG808_001458"
     CDS             complement(15249..15791)
                     /gene="rimI"
                     /locus_tag="ACG808_001458"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFS5047088.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(15775..16497)
                     /gene="tsaB"
                     /locus_tag="ACG808_001459"
     CDS             complement(15775..16497)
                     /gene="tsaB"
                     /locus_tag="ACG808_001459"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFS5047089.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDKIIVPMFDARRKNVYAGAYRWKENRLEIVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFYEIKEILPSAMINTVPAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(16688..17668)
                     /locus_tag="ACG808_001460"
     CDS             complement(16688..17668)
                     /locus_tag="ACG808_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5047090.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSDSKAPVSRKIIVETELIIRESTK"
     gene            complement(17692..19176)
                     /gene="galT"
                     /locus_tag="ACG808_001461"
     CDS             complement(17692..19176)
                     /gene="galT"
                     /locus_tag="ACG808_001461"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFS5047091.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(19188..20177)
                     /gene="galE"
                     /locus_tag="ACG808_001462"
     CDS             complement(19188..20177)
                     /gene="galE"
                     /locus_tag="ACG808_001462"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFS5047092.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         5623 a         4249 c         3595 g         6775 t
ORIGIN      
        1 ataaaagaaa tataaataaa aacagaggaa gcggaatgat ccgcccctct gtttttatga
       61 ttttattaaa cttccaataa ttctttttct ttatcagcag cgatcttatc tacttcttta
      121 atgctgttat ctgtcaatgt ttgtacgtct ttttctaatc cgcgcaaatc atcttcggta
      181 atatcaccat ttttttgttg ttttttatat tcatcgatcg catcacgacg gatgttacga
      241 atcgcgattt ttgcattttc tgcttctttt ttcacatctt tagctaattc ttttctgcgt
      301 tcttctgtca gttgcgggat aactaagcga atgacatttc catcattggt tggactgatt
      361 ccgatatcac ttgccataat agctttttca acatcttgaa gaatgctctt gtcaaaaggt
      421 gtgatcatta aaacacgtgc ttctggaatt tggattgatg ccatttggtt caatggtgtt
      481 ggcgcaccgt agtagttcac agtaatacgg tccaaaaggc tagcgttcgc acggcctgcg
      541 cggatttgcc caagttcacg ttgaagattt tgtgctgctt ttttcatttt ttcttttgct
      601 tctgtcataa ttgcatctgc catttttatt tcccccttac agttgttccg atattttcgc
      661 ctaagattgc acggcgaata tttccgtgtt catttaaatt gaagacaagc agtggaatat
      721 cattatccat gcttagtgaa cttgcagttg aatccatgac ttgtaatcct ttggcaataa
      781 cttccagatg agtcagttct tcaaatttta ctgcatttgc atctactttc ggatcagcag
      841 aataaacacc gtctacattg tttttagcca tcaggataac atctgcgccg atttctgccg
      901 ctcgtaatgc tgctgttgtg tctgtcgaga agtacgggtt gccggtaccg cctgcaaaga
      961 taacgatccg tcctttttct aaatgacgtt ctgctttacg gcgaatatat ggctctgcga
     1021 tttgacgcat ttcaatcgaa gtttgcacgc gtgtcggtac acccacgttt tccaacgtat
     1081 cctgtaaagc taaggcattc ataactgtag ctaacatacc catgtaatca gcttgagcac
     1141 gttccatgcc catttgtgct ccgatttgtc cgcgccagat atttccgcca cccacaacga
     1201 ttgccatttc aacgcccagc tcgtgaactt ctttgatttc ttggatgatt tctttgatta
     1261 ctggtggttt gattccaaat cctgcatccc cagctaaagc ttctccactc aattttaaga
     1321 ctacacgctg atatttcggt gtgaccatgt tcattcctcc aatattttct attgccattt
     1381 taccatactt agatgatttc gacactctaa gactggtttt tcttgttttg cttaaaaaaa
     1441 gggagcgcac aaaccgtgcg ttcccgtctg ttattcaaca gaattatttt ttcacttggc
     1501 tcataacttc ttctacaaag ttatcttcgc gtttttcgat accttcgcca acttcgaaac
     1561 gtacgaatgt tttaacagtt gcgccttttg atgcaacgta tttttcaaca gtcatatcag
     1621 gatctttaac gaatggttgg tccaccaaag cgatttctgc tttgaatttc ttcaagcgtc
     1681 cttctaccat tttttcaacg atattcgctg gtttgccttc gttcaatgct tgttctgtaa
     1741 gaacagtttt ttcatgttct aattcagctt ctggaatttg tgattcgttt acataacgag
     1801 gattgatagc tgctacgtgc atagctacgt ctcttgcaac tgtttcgtca gttgttccat
     1861 ctagaacagt caatacggca atacgtccgc ccatatgcaa gtatccacca aatgcagcat
     1921 tgtcgtcttt ttctacaact tcaaagcgac ggaagctgat tttttctccg ataacttgag
     1981 ttgcttcgat caagtcagat tcaattgtac ctttatctgt tttgattttc attgcagctt
     2041 ccatgtctgc tggtttgttt tcagcaacaa gttctgcaat ttcttttact aaatcttgga
     2101 acatttcgtt tttagaaaca aagtctgttt ctgagtttac ttcaacgatt gctgcagtgt
     2161 tacctttcac agcaactgaa gccaatcctt cagctgcaat gcggtcattt ttcttcgcag
     2221 cctttgccat tcctttttca cgtaaaagat caactgcttt ttccatgtcg ccttctactt
     2281 caactaatgc ttttttcgca tccatcattc ctacgccagt catttcgcgt agttctttta
     2341 ccattttagc tgtaacgtct gccatgttta actcctcctg tgtgtttttc tttttaaaaa
     2401 aagctgcttc aaaggagagt gaggcttgtc gcccagcctt tgaaacagct ccatcattca
     2461 aaattattct gctgaagaat tgtcgccttc aacaacatct acgatttctt cgattgatgt
     2521 tgctgtgttt tcttctgtga aatcttcttc aactacttga tcttcacctt ggtttccttc
     2581 gatgaaagca tcagccattt ttgaagtaat caatttaacg gcacgaattg catcgtcgtt
     2641 tgaagggatt actacgtcga tttcatctgg atcgcagttt gtatcaacca tagctacgat
     2701 tgggatattt aatttgtgtg cttcttgaac agcaatacgt tctttacgtg ggtcaacgat
     2761 gtacattaca tctgggattc ttggcatatc agcgataccg cctaagaatt tttcaagacg
     2821 ttcacgttct ttgttcaaac cagcaacttc ttttttaggt agaacttcaa aagttccatc
     2881 ttcttccatt gcattgattt gtttcaaacg gctgatacgt ttttggatag tatcccagtt
     2941 tgtcaatgtt ccgcctaacc aacgatggtt aacgaagtat tgtcctgcac gagttgcttc
     3001 ttctttgatt gcttcttgtg cttgtttttt tgtacctacg aataatgcta cgccgccttc
     3061 ttcagcaacg ctcttcatgt aatcataagc tgcatctact aatttaactg ttttttgcaa
     3121 gtcaatgatg tagattccgt ttctttctgt gaagatatat ttcttcattt ttggattcca
     3181 gcgacgagtt tggtgaccaa agtgtacgcc ggcttctagt aattgtttca tagaaattac
     3241 tgccatgttt gtttcctccg atttggtttt atttttcctc ttcttatctt ctaccttgcc
     3301 ggcaaacttt ttcaagcacc taccgacaat ccaataagaa tgtggatttg ctgatgcata
     3361 aagcaccctc agctattata cagacaaaag atccgtatcg caagagcaaa tatacttttc
     3421 tgctcaacat tcaatagttt acctgaaaga taaaaagaat tcaagaaaaa cagtagattt
     3481 ctctttgttt ctttattatc ctttttccat aaaataattt taggtataaa agacgaatat
     3541 tattgggcaa tccttctttt ttttgttatg atacatgtga aatctttcgt aaaggagaaa
     3601 gaaaatgcgc aaagctcaaa gacatttgct gattaaacaa gtcattgaag aattcccgat
     3661 ccgtacgcaa gaagaactat taaacaaact tgggcattat ggcgttaccg ccacgcaggc
     3721 aacgatttct cgagatatcc gtgatctaaa aatcgtgaag gcacctgatg aaaatggcgt
     3781 ttcccgcttc gtattgtttc gcgaaaaagg aactcctgag actaggactg aggaagagga
     3841 tcggctggtt caaatgattg aagacattgt attgaaagtc gaacgagtcc actttttgac
     3901 gatcgtcaat accttgccag acaatgctca tttatttgca tctgttttag atgagatcaa
     3961 acctccctct attgtcagta caattgctgg attcgatacg atcatggtca tctcggaaaa
     4021 tgaggaggac gcaaaacgag tagaagaatt tcttcaccat ccaactgaag tatcactttt
     4081 ttaatcagag cacgtattcc taatatggaa tgcgtgtttt tttattttca atattttcct
     4141 tattaaataa acctttacac tctttacaaa aatacatcat tttttattca attttttcaa
     4201 caccttgatt gaatcacaat aatcatcatt tatattggat gttaacaaga gcagcttctt
     4261 gctctttgtg tatttcaaaa aagaaatgaa aggaaatcat atgtataaat tattaactcc
     4321 tggaccattg acgacttctg aaacagtgaa aaatgctatg ctgatcgatc attgtacttg
     4381 ggatcaagaa tacaaagaaa ttacacaatg gataagaaaa aaattattgg atcttgctca
     4441 agtatcggaa ggaatataca caacggtctt aatgcaaggt agcggctcct ttggggtaga
     4501 atcggtatta agttctgtga tcaggccaga agatactgta ctgatttgtt caaacggcgc
     4561 atatggacaa cgaatgatca acatgtgcga atgtctgaaa ctgaactata ccgtttatga
     4621 ggtatcggaa catcaaatcc tccaagctca tgtgattaca cagctgattg aagagaatcc
     4681 ctctatctcc gcactagcaa tggtccatag cgagactact tccggtattt tgaataattt
     4741 ggaagagatt gctgctgtca cacaagaaaa agatcttttg tttatagtgg acgcgatgtc
     4801 aagctttggt gggataccga ttcgtgtagc cgaactaggg attgattttt tgatttccag
     4861 ctcaaacaaa tgtattcaag gtgttccagg cttttctttt gtacttgcta ataaagcgga
     4921 actagaaaag cgaaaaaatg tgagtcgaag tttgtcactt gatctatatg accagtaccg
     4981 cgtgatggaa aacgagaatg gcaaatggcg tttcacttcc cctacccata cggttttggc
     5041 ttttcaacaa gcgctgaagg aactagaggc agaaggagga atcgaaactc gatattcacg
     5101 ttatctcgcc aataatcatg tactacgaca aaaaatgaca gagatggggt tccagccttt
     5161 cattcaagaa tatcaggggc cattcatcac ctcttttctt taccctaagg actggcgatt
     5221 tgattttaat catttttatc agtatttaaa gcaaaacgga tttgtgatct atcccgggaa
     5281 aatcagcgat catgaatgtt ttcgtatcgg aaatattggc gagatctatc cggaagatat
     5341 tttggaactg acagcaatta tcaaagacta taaggagaac gttaatgatt aaaactgtaa
     5401 tttatgattg ggcaggaaca actgtggact ttggctgtat ggctcctgtc cacgctttta
     5461 gaaatgcttt tctagaaaaa ggaatccaac tgactgacaa agaaatccgc gaaccgatgg
     5521 gaaaattaaa atgggatcat atccaacagc ttctttccct cccttctgtc aaaaaacaat
     5581 ggatagctat ttatggacaa ctacctcagg aaaaagaggt ggaggaattg tatcaacaat
     5641 ttgaacatta tctgtttaaa gatttggtca agcattctac cttgaaaccc gacacattgg
     5701 aaacgatcga acggttgaga aacgctggga tcaatattgg ttccacgact ggatttactg
     5761 ctcctatgat gacaatcgta gcagaaaccg caaaaaaaga agggtattca ccagacttca
     5821 tggcaacttc tgaagacgtc gaagggtatg gtcgaccgtt tccttatatg atccataaaa
     5881 acatgcagta cttcaaaaac aagtcggttg atgaagtaat caaagtcgga gatacggtct
     5941 cagatatcca ggaagggaaa aatgccggag tgttgacagt aggtgtaatt gaaggtagtt
     6001 cgctaatggg gatttcttat gatgagtacc aaaaattaga gatggggaaa aaagatcgtt
     6061 taaaacagtc tgttgctctt agattccaac aagctggcgc tgatatttgt gtaaacaatt
     6121 taaaagagtt aacagaatac attttgtacg ctgacaagaa tccagaatga cttttataca
     6181 aaatcagagg tcgtgaaaaa gctgtactgc atcaagtaat aagaacagcc aaacaaaaat
     6241 agctcctcat atttttcgtt tggttaggct tattaccgca gatgcaagct tttgaacacc
     6301 gtttattcga aaaaaaaaag ggagctgtga cacgactttt gtcacagtcc ccttatttac
     6361 aaacaaacag tgtttaagag acaacctctc cactaaacgg tgttcattca gctgactctc
     6421 ggtatttgct caaaaaacga taaaatttga aaagctattt tctcttttgt ttctctaata
     6481 ctcggatcag aacgctgatt tcacattctc tttttcttac atcttatctg gtgcatgaag
     6541 tcctagcaaa cgaaggtctt ctttcaataa tacagtcact gcataaacta gagctaagcg
     6601 tgcttctttt tgttcatcat cagctaaaat cttcgtatga gcatagtatt tgttgaatgc
     6661 ttgtgctaat ttgattgcat gtttcgcaat cactgacggt tcgtattttt ccccagcaga
     6721 caagaccgtt tcagggtatt tttgaactaa tttaaccact tcccagctgg tatcgtcatt
     6781 caatgcatag tctgctgttt ctgaagggac aaatcctgct ttttccaaca ggctgactgc
     6841 acgagcatgt gtatattgca cataaggtcc agtttctcct tcaaatcgaa caacttcttc
     6901 caggttaaag tcaaatgtat tcagtcgatc gtttttcaaa tcgtggaaaa tgactgcccc
     6961 aaccccaact tgtttagcta cagcatcttt gttttccaaa tcagggtttt tctcgctgat
     7021 ttgttcttta gcagattgga ttgcttcatt taatacttct tctaataaga cgatctttcc
     7081 tttacgagtt gataattttt taccaccttg cgtgatcaaa ccaaatggaa tatggcgcat
     7141 atcgtcagac cagtcaaagc ccatttcctt caagacagct ttcaactgtt taaaatgata
     7201 gctttgttca ttccctacta catatagaga ttgtttgaag tcgtaagtac gtttacggta
     7261 caatgcagca gctaagtcac gtgtaatgta tagtgtcgca ccgtctgact tcttaatcaa
     7321 cgctggattc aggtcataag cagataggtc aacgatctct gcacctttat cctcgttcag
     7381 caaatgtttt tcttctaaaa gtttgacgat ttcatccatc ttgtcattgt agaaagcttc
     7441 accgttcaac gaatcgaaac gtacttccag cagatcatag attttattga attctttcat
     7501 cgattcatca cggaaccatt gccataattg aatagcctct tcatcgcctt cttccagacg
     7561 tttgaaccaa gcacgagctt cttcatttaa ttctggttct gtttcagcta cttcatggaa
     7621 ttggacatat aaacgaagca attcattgat tggttctgct ttaacagctt cttctgtacc
     7681 ccattttttg taagcgacga tcagttttcc aaactgtgtt ccccagtcac ctaaatggtt
     7741 gatgcggatt ggctgataac cgattttttc cataatgaaa ccgatcgaat taccgataac
     7801 tgtcgaacgc aaatgtccca ttgagattgg tttggcaata ttaggagaag acatgtcgat
     7861 tggtactgtt ccttgattcc cgatattgct atccccataa tgttcttttt ctttcactac
     7921 tgtttgcaaa acttttttgc tgataagttc tttattcatg aaaaagttca agtaaggtcc
     7981 gacaacttcg attttttcaa agttggcaga atcgattttt tctgctaaat ctgcagcgat
     8041 ttgctgtggc gcttttcggt aaacttttgc taatgaaaag gctggaaatg caacatctcc
     8101 gtgttcagct gattttggat tttctagtaa ttgttcgact tgttccagtg ttaaatcatc
     8161 tttgacagcg tcatagacag cttttgcgac aagatcttta ttattcatct ttattcctcc
     8221 taaagtgctc ccttgattgt taaacaaaaa aaatctctag cgacggcata cttagtatgc
     8281 catcactaga gacgagattt cccgcggtac cactccaatt gtcctaaaaa aggaccagct
     8341 ccatctgata tcggtagacg accgtctgtt taaaacagaa ttctccaagt gcgcttcgtt
     8401 gtatcttctt cattcggctt ccaccacttc cgaattcgct agccaagaat gatacaagta
     8461 ctctcttgtt caacaaatct tagtcttatt aaactttctt gaattatatc agaaaaaaaa
     8521 acgaaaggct agtcgtttct gataaaaaaa caatccagat ggcctaaagg agaagaaata
     8581 ccacctggat agctataaat catttcgtaa atacgaaaga cttaacaaat taatcagcta
     8641 cgataatcgt tccttcttca gaagctaaaa gattctcgat attttctaaa gaagtgatga
     8701 ttgccttagc gtttggtttt gcttcaacaa atgcaatagc tgcctctact ttcggaagca
     8761 tgcttcctgg agcaaattgc ttttcatcaa tgtattgttt catttctgaa acagtaacag
     8821 tttctaattt cttttgatct ggtttttggt aattgacata cacattgtct actcctgtca
     8881 atacgatcag caaatccgca tcgatgattt cggctaattt ttctgaagca aaatctttat
     8941 cgattacggc ttctcgtcct tctaatccag tagctgtttc tacaacagga atcccaccgc
     9001 caccaacaga gactgtgata actccttgat cgaccagcgt ttcgatgaca cgtgcttctt
     9061 taatcgagat gggtttaggg gaagcgacta cttttctcca tccacgtcca gcatcttctt
     9121 tgaatgtcac agtggagtca gcattcattt gttctttggc ttcttcttcc gtgtagaatg
     9181 gaccgactgg tttacttgga tttttgaatg aaggatcatt ttcatccaca ataacttgag
     9241 tgactaaaga aaccacatct ttatcgatcc ctttttcctt cagtacttcg cccatggcat
     9301 tttgtaacca gtaaccgata gatccttctg tcattgcaac acatgtatct aaaggcattg
     9361 ccggtgtttt ctcagaatca gcagcttgct gctgaatcaa taggttaccg acttgcggac
     9421 catttccgtg ggagatgatc aattcatctc cttgttcgat aaatttcact aaatactttg
     9481 ctgtttccat taacgcttct tgttgggctt ttgcacttgc atccgtagac aagattgcat
     9541 taccccctaa agcgacaact acttttcgtt tggccatttc attcgtcctt tcttcagttc
     9601 taccgaattt tctagataac aaaacgagcc agcgttttgt gattttccct caatgctgac
     9661 tcgttttttc gtgagatgaa tgtggttatg cttaaacgcg agggataaac aagttaccta
     9721 atgttgctgc catgatcgct ttgattgagt gcatacgatt ttctgcttgg tcaaattgac
     9781 gagcatattt gctgcggaag acttcatctg ttacttccat ttcagtgatg ccaaagcgtt
     9841 ctttcatttg ttcaccgtaa acagtgtttg tatcatggaa agctggcaag cagtgcaaga
     9901 agatcaaacg atctgtgttg tgtgtttttt ctaccatttc catattgatt tggtaaggtt
     9961 tcaacaattt gatacgttct tcgaatttat cttcttcacc cattgatacc catacatcag
    10021 aataaaggac gtctgctccg tctacacctt tatcaacatc gtcggtaatc atcaattgtg
    10081 caccggattt ttcagcaaag ccttctgcca ttttgacgat ttcttcttca ggttgtaatt
    10141 cttttggtgc aacgatacgc atattcgctc ctaaaattgc acctgtgact aataaagagt
    10201 tagccatatt gtttcgacca tctccgcagt aagcaacagt gataccttct actgttccaa
    10261 agttttcttg gatagttaaa aagtcagcga tcatttgtgt tggatgccat tcatcagtca
    10321 agccattcca aactggaaca ccagagaatt ctgctagttc ctctaccatt ttttgactga
    10381 atccacggaa ttcgattcca tcaaacatac gacctagtac tttagctgta tcttctgtgg
    10441 attctttttt acctaattgg atatcatttg cacctagata ttcaggatgt gcacctagat
    10501 cgatcgctgc agtagtaaat gcagaacgag tacgtgtaga tgttttttca aataaaagcg
    10561 caatattttt tccttctaga tagtgatgtg ggataccacg ttttttcaag tctttcaagt
    10621 gttctgaaaa atcgatcaaa tattgtaatt cttcttttgt aaagtctttt tctgctaata
    10681 agcttcttcc ttggaatact gattctttca tattaaacat tctcctttag attgttttta
    10741 tttaagatta aagaggaaat cccgacggca gctgacagta agaccgccgt caggaaacaa
    10801 attacttcaa gtcttcacgg acaagtggtt ggctcatgca gcgtggacca ccacggcctc
    10861 gtgacaattc acttgaattg atttctaaca ctttgatgcc atagctacga agcaattcgt
    10921 tcgatacata atttcggtta taagtgacta caacacctgg agcgattgcc aatgtgtttg
    10981 atccatcatt ccattgttct cgaggtgcaa cgatctcatc tccattacca gttgggatca
    11041 gtacaagatc atctaatcca agagcagctt tcatcgtttc gtgtaagtta tctgaatgag
    11101 taatcttgat atcatcacca tctggttcga ttgtaaagac atccatctta ccgtctttac
    11161 tttgaattgc tggatggata gtgaatttat cgtgatctac cattgtaaat acggtatcca
    11221 aatgcatcat tgcacggttg tttggaattt tgattgctaa cactttttca aatcctgaat
    11281 tttttgcgaa taggtggcgt gctaattttt ctaaagcttt cgcattagtt cgttgagaaa
    11341 tcccaacagc tactacttta tcgcttaaga tcaattcgtc tccgccttcg atatgatctg
    11401 gattttcacg atccaaccat acttcgattc ctttatttgc aaatcttgga tgatgtttca
    11461 aaatatattc tgtaaacatc gattcacgac gacgtgcttc aaaagtcatc ttattgactg
    11521 tcatcccatt tcccattgat gcggaagggt cgcgtgtaaa gtacaagttt ggcattgggt
    11581 ccatgtaaaa tggatagtca tcgtctgctg acagatcgta tagactgcta gaacgtactt
    11641 caatatcttt tgtacgaacc ccagccatga ttttatctac catttcttgc gtttccattg
    11701 atagaaggaa ctcatgaaga ccctctctta cagcattcga tgcaatatga gattcatcca
    11761 acattttatt caagaattgt tctttgacat tgcctgcgtc aatcgcttct gccgctaatt
    11821 tttccaagta aagagtctcg acgccttcat tttgcaatgt ctttgcaaag ttgtcatgtt
    11881 cttcttgagc aatcggaaga tatggaatat catcgaacaa cagacgatcc ataatatctg
    11941 gtgtcaaatt ttccacttct tgtccaggcc ttttcagtaa aactgtcttt aatttcccta
    12001 tttcagagaa aacgtgaata ggtttatcca tgttgtgttg cctccttctt tttgattaca
    12061 tgtataggat atcctccttt tgtaatcact ttcataattt tgtctcattc aaaatgagac
    12121 attcctcaca ttaatattcg ataattaaat aattaaacgt ttttctattt ttaagcgtaa
    12181 tttattcatt ttttattctg tatacacttt caaaaaaata tttattcatt gaaaaaaatt
    12241 ttttgaataa attaaaaaaa taaattatat tagcgttgtt agcgtttact atatgtatta
    12301 ttgttgtttt ttatacacaa atcattcgct tcaaaacaaa ttattcaata ccattgtata
    12361 ttttttacat aataacaaat tatatttgta aagtattaaa acttgtttct aatgttggta
    12421 tgttaaaact ttacaaattc tttaaaaaaa gtgacaataa atatagtgaa caataaggag
    12481 aggacttttt aatgcgtgtt aataaaatcg attttcactt ttcaaaactc agatatcaac
    12541 ctgattttga acatttctcg gatgaagagt tccaacagat caaggaacat actgttcttc
    12601 gttcttataa aaaaggacag atccttttcg acgatggtga tcccagacaa cggttgtata
    12661 tcttgaccaa aggactcgtt cgtttagaac gttatgatga aagtgcaact tattactact
    12721 atgactatgt gaatacaaat aacctttttc ctatgtcagg aatgtttgga gaagagacct
    12781 acacttatac agctcaagcg atgactgata ttgaaacatt ctaccttcct gtttcctact
    12841 atgaaaaatt agcacgaaaa aatcccgaac agttgatcta tatgatacaa aaaatttcta
    12901 aagtagttga aatacatgaa ttgcgtatcc aaatcggatt gacctctagc gcctttgacc
    12961 gggtaaaaca aaatttattc attttgaaag aagaattggg cgtaccaaca aaagatggtc
    13021 atatcaccat tccttatccg atcaccttaa aagaattagc agtgaatagc ggcacgacac
    13081 gtgaaactgc tggacaagta atcaaacaac tacgtgattc cggactgtta gattatgaca
    13141 aaaagcgttt tactttctgt aagacagcta ctactccagc tatgagagaa taacgcgaac
    13201 aaaaataagc agtgaccctg atatttatca gggtcactgc ttattttcct ccctaaaact
    13261 tctgaaagat ccggcagttt tagatcactg ggcaagactc aaagattttc tatgtgtgtt
    13321 tcagttatgt aatattcact gtttgcaaaa aatactgata gattaactag aggaagcgtc
    13381 ttgtcctcat ccgacctaga ttattcgata ttcctcgaca ataaacgatt aatcagtgag
    13441 ggcaatcaga ggaacggata atgatgaata agctgtaaaa aatagggctt tgacaaaagc
    13501 gtgtcacagc ccttatttcg attaaacggt gtgtacctgt caactcttcg gtcacaagtc
    13561 ataatgttca taaacatgag gagctgtttt ttgaaattgt tccttgtgaa tcagagttaa
    13621 acgacaggct tacgaactct tgattaaacg gcgttcaaaa gcttgcatac gcggtaataa
    13681 gaacagccag gtcaaaaaca tgagaagccg tttttcctgg gctgttctta ttacttgatg
    13741 cagggcagat ttttcacaac ctctttattc agataaaacc aagcttggat cagcattcaa
    13801 ctgatagcta gcaaaatttc ttttttgcat ttccacatgt gctgctgctc caatcattgc
    13861 agcattatct ccacaaagtc gaagaggggg aatcactagt tctacctcag ggagtttagt
    13921 tgccaaagca tcctgcaatc cttcacgcaa cccttgattg gccgctacac ctccagctac
    13981 aatcagttgc ttgactggat aattttgaca agctcgaagt gttttagtaa tcaatacatc
    14041 gatcacactt gcttgaaagc tagctgctaa atcatttttg tctagatctt ctccacgctg
    14101 ttgtgcatta tgcaccaaat tgataaatgc actttttaat ccgctaaaac tgaaatcata
    14161 attatcttca tggatcatcg ctcgtggaaa atggtaatta tcttttccct gatgagctag
    14221 ctgatcgatt tcttttccgc ttgggtaaga aagacctaat actcttccaa ctttgtcata
    14281 agcttctcct gctgcgtcat ctcttgtctc cccaatgatt tcatatgatc catcttcttg
    14341 catatagact aattctgtat gtcctccgct aacaagaagt gccatcaatg gaaactggaa
    14401 tggtttaacc agacgggctg catagatatg ccctgccata tggtttacag gaataagcgg
    14461 caattggtgt gcccaagcaa aagccttagc tgcagaaata ccaatcagca acgagccgac
    14521 aagtcctggt ccataagtga cagcgactgc cgataaatct tctgccgaca caccagcttc
    14581 taccagtgca tcttccaagc ataaggtgat ttgttctacg tgatggcggc tagctacttc
    14641 aggaactacg ccaccaaaac gtttatgact gtttatttga gaagcaacga tgttagataa
    14701 gatctctgtt ccatttctaa cgaccgctac actcgtttca tcacaacttg attcaatcgc
    14761 cagaatcaat tcttcattca tttcatcgtc tccgttttta actttctgct cataatgatc
    14821 gcatcttccg tcggattgcg ataatatgat ttccgtttac ctaatatacg aaacttttct
    14881 gattcataaa gttttcttgc tgcttcatta gatagacgta cttctaaaaa aacatgcagc
    14941 gaatctttta tttgttccgt aatcagtaat tgtaacaaat gccgtgcatg cccttttcct
    15001 tgttctgaaa ctgcaacagc aatatttgtg atctcgactt catctattac tttcgagtat
    15061 ccaataaagc cacagatctg gttatcttca actaaaagca gataatcaga gtgaggctgt
    15121 tggatgtctg ccaaaaactg cattttcgtc caaggtgaac catgtggata agcattttcg
    15181 ctaatcatcc atacgcattc tgccaagtac gcttcatcaa aatctttttt tctgcaaatc
    15241 aactaatctc actcattttc taaaagtttt atcatatcca acgcatcttc ttttgtttca
    15301 gtgtaatatc tttttttgat cgttgttgct tgaaaaccta acttccgata aagtctttgt
    15361 gcatcttgat tactcatacg cacttctaat gacatcgtct cacagcgatt cattatagca
    15421 aagttctcga tttcgtcaat aagcagcgaa ccgattcgtt tacgctgata tgccggtcga
    15481 acggcaatat tcgtgatatg gcaatctaaa ccgaacgctc ttgaaccgat aaaaccaatc
    15541 attcttccat tatcaaagat acctagatat agatgaggaa gcggtgactt caattctgat
    15601 ataaaggcac tttttgtcca tggcaattcg ccattataga cttctcgttc gacatccagc
    15661 agctctttga tatcttctgt agtcaattct cttagtacgt aacgacgatc gatttgttcg
    15721 attgtttttt ctgtataaat cattttatct tgaaaaaagc ttctggcata cgctctaaat
    15781 tttttcaaca taactttcgt ctccaggtgt gtgcgtctcc aaccattttt cttctgcttc
    15841 tacccgtttt aaatagcgag gaagaaaagc ttgtttctct gcaactggtt cagctaaagc
    15901 acctaattga gccacagtta caccacttgg aatatcccat gctggaactg tattaatcat
    15961 cgcactaggc aatatttcct ttatttcata gaagaatttg tgacaatcac tcccgacgaa
    16021 ataaatagct tcattcagct tttgtagttg tgtgaacaaa tcagaagcag aaatatgtac
    16081 atcttcaagt acaatttcta atctattttc tttccagcgg tatgccccag cgtatacatt
    16141 ttttcttcga gcatcaaaca tgggcacaat aattttatcc acaccgatgc aattagccgc
    16201 gatggttttc agactagata ctcctactag ctctttatct aatgtataag caatcgtttt
    16261 tgctgtcgtt acacctatac gaagccctgt ataagaaccg ggaccatcag aaaccgcgat
    16321 tcggtcaatg tcttttggac ttatttgaag atctgcaaac aattggtcga ttgcaggcat
    16381 cagtgttaca ctgtggttgc gtttgatatt tgtttgtatt tgtccaagaa tttgttcgtc
    16441 ttctaccaca ccgatcgcca aagtttggtt tgcagtatct attcctaatg taatcatata
    16501 tttgatcctt tctgctatga cattctcgcc ttccattgta gcatactttt ttattctgag
    16561 gatgaaaata ccaaaggcaa aactagtaca atgatacatg attatctgct ggcgggcaaa
    16621 ttttgcccct tcctgaaaca caaaaaagct gcggtatcac ttcatttccc cacagctttt
    16681 ccatctttta tttcgttgat tctcgaataa tcaactctgt ttccacaata atctttcttg
    16741 aaaccggtgc tttggaatca cttaattgaa gaatcgtctc cactgctagc tctcccatcc
    16801 attcggtata aacttttaca gtagaaagag agggcgttac atatttcgct acactgacat
    16861 cattgaaacc catcacagaa atatcttctg gtaccactaa acctttttct tgaatcgctt
    16921 tcagtgcgcc aatcgctaat gcatcattag ctgcaaaaag cgcatcagga acatgcggat
    16981 tcgtttttaa atactgcttc actgcttgat atcctgactc gacagaaaaa tctgcttcga
    17041 taattggacc ttcgtcgtag cctttttctg ccaaaacttg ttgaaatgcc ataattctgg
    17101 gatcttttag ccgctgatgg tttttcttcg tatattctgt tccagcaagc atagcgatat
    17161 gttgcgcttt atgggagaca aaatgttcaa tgactttttg aacacttgac ttaaagtcga
    17221 caactagaga gtcgattcct tgagaagtgc cgtcagaatc taccaaaagc aaggcatttt
    17281 gggatccttt catttctaat aactgagctt cgtcgaattt tcctagtgcg atcgttccat
    17341 ctgtcggaag atcactaatg gaatcccacg tttctttcaa taaagtaacg tttgcttctt
    17401 ctgccttttt ttcgattcct agacgaatag ataaataata aagatcttca agctcttccg
    17461 tatcgtcgta ccactggatc aggcgaagcg tttgcttagt atttgctgca tttttacgat
    17521 gtttcgtata atttaagcgt tctgctgctt caaaaatttt ttgctttgtg gctaagccaa
    17581 cggaaagatc aggatcatag ttgagaactc ttgaaacagt agcaggagaa actcctgcca
    17641 actgcgcaat gtctttaatt gttgccattt gtctctcctc cttttttacg tttataaaat
    17701 tgcagtaaat cgttcaaacg ctgcctgtcc tttttcatct cgcttgaata ctccagcgtc
    17761 ttccagtaca cgtgcaaaaa cctgacctac agatttttgt acgacatcgg tgaccgtatc
    17821 tttcgtcaaa tcaggatgtt ctttttttaa ttgttctgcc cacggacgat gatattcagc
    17881 catctggttt tcttctccta ataagaaatg ctctacttct actagttctg gtttcaaacg
    17941 aggtggtaaa acagcaagcc ccattacttc gatcaaccca atgttctcct ttttgatatg
    18001 ttggacatct ttatgtggat gaaaaatccc atcaggatgt tcttctgaaa cattgttgtc
    18061 tcgaaggaca aggtccaact caaagtaatt gtcccttctt cgtgcaattg gcgtaatcgt
    18121 atggtgtggt gtaccatctg ctgaaaaagc tctgatagat actgtttcat ccgaatatgc
    18181 tttccacttt tccaatactg agttagctgc ttcaatcaac aattttttct ctgttgattg
    18241 caaacggatc acagacatcg gccatttcac gactcctgcg atgacttgcg gataatctgc
    18301 tagtttgata agtttttcta tcggtgcttt tgccatagga aattcatgac gtcctgcctg
    18361 ataatgatca tgagaaagaa tcgatcctcc aacaatcggc aagtcagcat tcgaaccaac
    18421 aaagtaatga ggcaagactt cagtaatccg aagcagtcta gcaaatgttt cctttgtgat
    18481 tttcatcggg cggtgttgtt ctgataaaat gatcgaatgt tcattgtaat aagcataagg
    18541 agaatattgg aatccccagc tttcaccgtc tagattcatt cgaataatcc ggtgattggt
    18601 gcgggctgga tagttcaagc gacctctata tccttcgttt tccatgcaaa gcatgcaggt
    18661 aggatagtct acttttaccg cctcgcgttc tgctgcgatt tgttttggat ctttttctgg
    18721 cttagataaa ttgattgtga tttccagttc accatactcc gactctgccg gaaatttgat
    18781 gttttttgcg atggctcttg ttttgatgta gtcatttttc ttacttaaat taaagaaata
    18841 ctcagttgct tcctctgggc ttttcgaata gtgctgtgca aagaatgcat tgacgactga
    18901 aggtggcggt gtgagaaaat ccattaattg cgcttcaaaa atctcttgtt ctgctggcaa
    18961 ttctgaaaga acaccatttt ctcttgcctt agcgacaagc tgatccaata agtctacaga
    19021 ttcttcctta actggtctta cttcaatgct ttctagagca tcttctccta ccatccctaa
    19081 aagacgattc tgtaaatata agcggtccat ttccatccat ccgccagctt cgatcgcaag
    19141 tgtggcaaaa tcacaaatcg tttgactgat tgacatatct cattccccta ttcttcataa
    19201 ccatttggat ggctgacatg ccaatcccat gctgttttga taattgcttt gatatctgtg
    19261 tattcaggtt tccaacccag aacctctctc gctttttcac ttgaagcgac taatcggcta
    19321 ggatctccag ctcttcttgg tgctatttct gctggaattt cttttcctgt cacttctcta
    19381 gctgcttcta gcatttcctt gactgagtat cctttgctgc tgcctaaatt gaagaagttg
    19441 ctttcatttc cttctttcaa gtattccaaa gctaaaatat gtgcagcaat caaatcttct
    19501 acttgaacat aatctctgat acatgttcca tctggtgtat cataatcgtc tccataaaca
    19561 gccaatgctt ttctttgacc aagagctact tgtaaaatga ttgggacaag atgcgtttct
    19621 ggtgtatgat cttctccgat agatgcatct gcttttgctc cggctacatt gaagtagcgc
    19681 aaagcgacat agcgcatgcc atatgcttgg tcacaccact tcatcatttt ttccatcatc
    19741 aatttacttt caccatacgg atttttagga ttagctggtg tatcttctgt gattggagac
    19801 tcttttggtt ctccgtaagt tgctgcagta gaagagaaca cgattttatt cacattgaac
    19861 tcgtgcatga cttcaagtaa gatttgcata ccgtatacgt tgttgttgaa atacatcaat
    19921 ggtttttcga cagattctcc tactaaagag cttgcagcaa aatggattac tccttcaatc
    19981 ggttcctttt caaatacgga acgtagaaat tctttatccc ggatgtctcc ttcataaaaa
    20041 tgagcatctg gatgtactgc ttgtttgtga cctgtgagca aattatctac cacagctact
    20101 tgatacccct tttgaactag ctggtctaca gcgtgcgaac cgatataacc ggctccgcct
    20161 agtactaaaa ttgtcataaa aaacactcct ttgattagta aaattgtgtt tgttgattat
    20221 atataattta aaaaaacgaa gt
//
LOCUS       DAZUMK010000043        19896 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000043 DAZUMK010000000
VERSION     DAZUMK010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.043"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..686
                     /locus_tag="ACG808_001463"
     CDS             <1..686
                     /locus_tag="ACG808_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS5047093.1"
                     /translation="LYNVAFILLNLLISVVFAIIMSELRNKRLVKVYQTMSLLPYFLS
                     WVIISYFVYAFLSPDKGIFNQWITGHGGEAVNWYNEPKYWPFILVFIGTWKGIGYNSI
                     IYFASVMGIDPTYYEAAMVDGASKWQQIKHVTIPQLIPLMTILTILAVGNIFRADFGL
                     FYNIPRNSGALYDVTQVLDTYIYNGLTSTGDFGMTAAAGLYQSVVGFVLLMITNTIAR
                     RFDSDSALF"
     gene            698..1618
                     /locus_tag="ACG808_001464"
     CDS             698..1618
                     /locus_tag="ACG808_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5047094.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1659..3116
                     /locus_tag="ACG808_001465"
     CDS             1659..3116
                     /locus_tag="ACG808_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5047095.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3461..4075
                     /locus_tag="ACG808_001466"
     CDS             3461..4075
                     /locus_tag="ACG808_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS5047096.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            4068..5792
                     /locus_tag="ACG808_001467"
     CDS             4068..5792
                     /locus_tag="ACG808_001467"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS5047097.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            5789..7240
                     /locus_tag="ACG808_001468"
     CDS             5789..7240
                     /locus_tag="ACG808_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS5047098.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(7713..9866)
                     /locus_tag="ACG808_001469"
     CDS             complement(7713..9866)
                     /locus_tag="ACG808_001469"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFS5047099.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(9868..10914)
                     /locus_tag="ACG808_001470"
     CDS             complement(9868..10914)
                     /locus_tag="ACG808_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5047100.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            11064..12350
                     /locus_tag="ACG808_001471"
     CDS             11064..12350
                     /locus_tag="ACG808_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFS5047101.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVE"
     gene            12366..15068
                     /locus_tag="ACG808_001472"
     CDS             12366..15068
                     /locus_tag="ACG808_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFS5047102.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            15053..15910
                     /locus_tag="ACG808_001473"
     CDS             15053..15910
                     /locus_tag="ACG808_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS5047103.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(15981..16838)
                     /locus_tag="ACG808_001474"
     CDS             complement(15981..16838)
                     /locus_tag="ACG808_001474"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFS5047104.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            17049..19220
                     /locus_tag="ACG808_001475"
     CDS             17049..19220
                     /locus_tag="ACG808_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFS5047105.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            19288..19629
                     /locus_tag="ACG808_001476"
     CDS             19288..19629
                     /locus_tag="ACG808_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFS5047106.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
BASE COUNT         6655 a         3523 c         4102 g         5616 t
ORIGIN      
        1 ttttatataa tgtagcattt atccttttga atctgctgat ctcagtcgta tttgcaatca
       61 ttatgagcga gttgagaaat aaacgactcg ttaaagttta tcaaacgatg tccttactac
      121 cttattttct atcttgggtc attatcagtt actttgttta cgctttttta agtcctgaca
      181 aaggaatatt caatcagtgg atcactggac atggaggaga agctgtcaat tggtataatg
      241 aaccgaaata ttggccgttt atcttggtgt tcattggtac atggaaaggg attggttata
      301 acagcattat ttactttgca tctgttatgg gaatcgatcc gacttattat gaagctgcta
      361 tggttgatgg agctagcaaa tggcagcaga tcaaacatgt gacgatacca caacttattc
      421 cactaatgac gattttaacg attttagccg taggaaacat tttccgagct gattttggcc
      481 tgttttataa cattcctagg aattccggtg cattatatga tgtgactcaa gttttggata
      541 cgtatatcta caatggcctg acatccacag gagattttgg tatgacggct gctgcaggac
      601 tttaccaatc agttgtcggc tttgtcttgt tgatgatcac aaatacgatc gcccgacgtt
      661 ttgatagcga ttctgcatta ttctaaggag gaaaaaaatg aaaaaaaaga aagtggagag
      721 agtccaagtc cgctctttca ataagacggc taattttttc tctaatataa tgattgcact
      781 ctttgctatt tcctgtattt tgccatttat atttgttatc gccatctctt ttaccgatga
      841 gtccatattg tcgactcacg ggtacagttt ttggccagaa aagttcagta tatttggtta
      901 tacgtacttg ttcgaccaga tgcaggataa aattttccaa gcgcttttcg tcactgtact
      961 tgttactgtt ttaggtacac tgatcaatag cacggctact tctttatatg cttatgcgat
     1021 ttcacggtca aactttccat tccgtcgttt ttttaccgtt ttttgtttga ttacgatgtt
     1081 gttctcacca gggatggtgg ccaattatct tgttatgaca aatcttcttc agttaaaaga
     1141 tacgatttgg gcattgatct tgccaatggc tgtcagtccc ttcaatatta ttgtgatgcg
     1201 cacattcttt aaacgttcgg tctctgattc gattattgaa tcggcacgga tcgatggagc
     1261 tagcgaactg cgaatatttg ttcagatcgt tttaccgtta gctgttccag gtattgcgac
     1321 aatcagttta tttgccgcat tgggttactg gaatgactgg ttcaatgctt tactatacat
     1381 ccaagatgat aagctagttc cactacagta tcttttgatg aaaatccaaa gtaatattca
     1441 atatctgaca caaaattcag gagcaggaag ccaattgaca ggcggattag cctcagttcc
     1501 aggagaatct gccagaatgg cgatcgtagt catctcgacg ttgcctattg cgatcagcta
     1561 tccatttttc cagaagcatt ttgtcaaagg tctaacaatc ggcggagtaa aagaataaag
     1621 aaacaaaaat ataaatcata aaattaggag gaagaaaaat gaagttatgg aagaaactag
     1681 cctttacagg tgtttcagcg ttgttgttgg gaacattagc agcatgcgga ggttctggtt
     1741 ctaaagacca ggcagaagct agcaattcgg acactttgca gatgtatcaa atcggagata
     1801 aaccagataa tttcgatcaa ttgatggaag tagcaaacaa acgaatcgaa aaagagatcg
     1861 gtgtaaaagt caatatcaat tacattggct ggggcgatta cgaaaagaaa atgaacgtca
     1921 tcatttcttc cggggaaaat tatgatatcg cctttgcgaa caactatgtt tccaatgccc
     1981 aaaaaggagc ctttgctgat ttgactgaat tagcgcctaa gtacgctgaa aaagcttata
     2041 atgatttaga tgaagcatat attaaaggaa atctggtcaa tggaaaatta tatgctttcc
     2101 cagtcaatgg aaatgtcttt gctcaacaag tacttacttt taacaaagct ttactagata
     2161 aatacgatct ttcaattgat ggaatccaat cttacgctga tgcagaaaaa gtcttgaagg
     2221 aattccatga aaaagaacca aacacggcag cttttgccat tggacaaggc ttcaaagtcc
     2281 aagggaactt tgattatcct ttagggaata cgttaccatt tgctattgat ctggatggag
     2341 atgattcgaa aatcatcaac caatacgata atgaaacgta tatcggactg ttacgtacaa
     2401 tgcatagctg gtacaaacaa ggactcattc caagtgacgc tgcaacgagc aataaggatt
     2461 atccactaga aggaaatact tggctaatgc gtggagaaac acaaggaccg tatgattatg
     2521 gcgatacaat tttgacaaat gcagcaaaac aagaacttgt ctcaaaagca attactgttc
     2581 cactaaaatc tacagctcaa gcacaaatgg caaattttgt cgtatcaaat acttcaaaaa
     2641 ataaagaaaa atcagtagaa cttctagggc tgcttaacag tgatccagaa ttattgaatg
     2701 ggcttgtttg ggggatcgag ggagaagcat gggaaaaagt cgatggttca gatgataaaa
     2761 tcaaattgct agatggttat cagccaaata cacatatgtc tgcttggaat acaggaaaca
     2821 acaagattct atatacacaa gagtctatta cggatgacat gattgccaaa cgtgatcaat
     2881 cgattgctga tgcagaaact tcaccgattt taggattcag cttcaacact gacagtgtca
     2941 aaacagaatt aagtaatatt tctaatgtca tgaatcaata tttagatgga ttgaatacag
     3001 ggactgttga tccagatgaa acattaccga aattgaaaga cgctttggac aaggctggct
     3061 atgataaggt attgaaagaa atgcaaaaac aatatgatga gttccgacaa gaataataat
     3121 gaataaagac aaaggggttg tgacaaaaga cgtgtcacaa cctcttgttc cgaataaacg
     3181 gtgttcaaaa gccagtttct cggtattaag ctaaatatat gtaaaaatgt ggaaagccat
     3241 attcacaaaa tttttcttac tactcgaaac taagcggcta tttcacaaac tcttggtaaa
     3301 cggtgttcaa tcagctaacc ctcggtattc gctcacaaaa cgacaaaata tgaggaacta
     3361 tttttccgtt tgtttctcta ataatcaggt caaggtgctg attgcccaac ctttttttgt
     3421 ataatgagaa agtataaact taatgtttgg aggagaagat atgttaggga aggttttgga
     3481 agagttattt atccggatct gggtcattat aaaactaacc ttgtatttct ggatatatac
     3541 atttgccggc gggatcatct ttggattagg tgcggcatgg aaaacagtca atgaactatt
     3601 ttatttgtac ggattcgagt ataaagaaat cacaataaaa agaggctgga atatatataa
     3661 aagaaatttt ttaagaggca atctgctatt tagtttattt ttatctggta ctgctttatt
     3721 gagttacaat ttatatttat ctgttcaaat caaagggctc atttttttag ctattgattt
     3781 tattttactt tttgccttgc tctgcctatt tgctacttat caatacagta tgattttaga
     3841 tagtgaatat acgatctctg ttcctaatct gctaaagcta agttttattt cggttttttc
     3901 cagcttttcg tcttttctaa aaataattat tggtagcggt ataattttag gcgtgacttg
     3961 gcaattcaaa ggattgatcc tgtttggtgt cattggtctt ttgacggttt ggaatggtac
     4021 gatgacaaca cattggcgtg aagaattaga taaacagttg gaaagttatg aataagtttc
     4081 ggattctatt ttcaaaagga accttactaa atcgtctgtt gcggaaatac gcggttatca
     4141 tgatcggtgt aacgatgact gctaccgtta tttttagtat ccatacttgg gaacaaaatc
     4201 aaaaacaggc agaaaacatg acaagtgatg ccgttcaaag cacaagcaga atgctgaatg
     4261 ataaaacaac cttgagtagg atcatcaaaa accagcttgt agggaattcc gaaaagatcg
     4321 aaaatgtgac gacctactta acaaaaccca tagatcagta tctgatgtat gtatacgaac
     4381 agcaaaacag taccgatgaa ttagtttctt tcccaaatca aatcaaagat ctctacgcaa
     4441 attatgaaga actaagtgcg atatatatcg tgttgaatca actaccggag tattatgaat
     4501 cttctagaga aaacaaaggt ggggaaataa aaaaggggac accaaaatta ctagacgcct
     4561 tttatatcaa gttgcctatc acacaaggag gagctgagtc aggaagtatt tttattggtt
     4621 ttcaaaagaa agaactagat atgattttag aaaatctaac gtcattcaat ggactctctt
     4681 tatacatgat ttctggaacg acaaatcgac tatacacatt ccatgataat aaaatagcta
     4741 aagaaacgtt tgaaagacaa gaaatgatca tcacagagag tctgaagaaa aattcacaat
     4801 tgccgattga cgatctggaa aaaaacaatt ttgttcaaca ccaagaactg tctgatgatt
     4861 atcgtatatt agctgtacta gataaaaaaa gtgtaaaaat cgaaacggga aaaaatcttg
     4921 ccccgctgat cattggtatc attattttag acaatatact gcttatcttt ctttataaaa
     4981 cattcaaacg atattcacag caagtatcta tcgtcatgca ggccatggat caaacagcag
     5041 cagggaattt agagacgcgg atcgacataa caaacacgga atatgaactg aaggaacttt
     5101 caatcggtat caacgaaatg ttggatagca tcaatcagtt tgtagaagac atctacaaat
     5161 tagaaatcaa acagcaagac gcacatatgc gggcgcttca agcacagatc aatccacact
     5221 ttttatataa tacattagaa tatatccgaa tgtatgcaat cagcgaagga agtgaagaat
     5281 tagcagatgt tgtgtatgcg ttttccgctt tgcttcgaaa caatacgaat caagagaaaa
     5341 cgatcacttt aaaagaagaa ctggactttt gcgaaaaata tgtctatttg tatcaaatgc
     5401 gttatcctaa tcgagtagcc tatcatttta tgatcgatcc tgatttggaa aagatcgaag
     5461 tacctaaatt tgtgatccag ccattagttg aaaattattt taaacacggt attgatttta
     5521 ctcgattcga taatgcgcta agtgtcaaag tcttgcagga aggcaaacgg gtaaggatca
     5581 tcatcaaaga taatggaaaa gggatgactg aaaaacggct aaaacagatt gaagaaaaac
     5641 tgtctcatcc taaagtggaa cttcacggtt ctatcggtct gcaaaacgtg aatgaacgtt
     5701 tacgtgccag cttcggcagc agttattata tgtcgcttga gaataatgag acaggtggac
     5761 tgaccgtttc tattacattc aaggagggat gagcgtgtat caagtgttat tggtagatga
     5821 tgaatacatg attttgaatg ggttaaaaaa gataattgat tggcaaagtt taggctttca
     5881 aatagtagca acagcagaga atgccaaaga aggactagct gtacttgaac agcgacagat
     5941 tgatttagta gtaacggatg tgacaatgcc agaaataaat ggactggaat ttattgaagc
     6001 ggctcaaaaa gaacgtcata attttgaatt tatgatttta tcaggatacc aagaattcga
     6061 ttatttgaaa ggaggtatgc aattaggcgc cgtgaattat ttgatgaaac ccgtgaataa
     6121 gttcgaattg gttgaaagcc tcaagaaaat caaaacacgg ttagatcagc aaaatgaaca
     6181 aaaaaatcaa caagaaatct atcaagaact gttattcagt caatggttga atgaagaatt
     6241 ggatgaaata agtgaagaag aaataatagg acaaataagt gagaagcagc ataggatagt
     6301 attgattcaa ctggcaagaa ctcatgatac tcttatcaat agatggttgc aagaacggca
     6361 acaaatcttt tactatcaac gaaattacgg agaccgtgta ttgatcacgt tgttgttgga
     6421 ggcagatgaa gttgatggct tctgttgttt tgttcaagaa aactttcctg atcaggaatg
     6481 gctgatcagt atcggggaag agacagattc tttggacaaa atccctgaaa cattccaaca
     6541 agcgaaagac aatctacagt tgcatcaatt ttatggagat aaagacgagc atgtttttta
     6601 cgcagtacaa gcagaaacaa aagatcaaac catcgatttt tcaagtttca gacgcgtgct
     6661 gcaaaataaa caattggaag tcgctcaaaa aatgatcact gatttttttg aacagtttca
     6721 gttagcagcc atgatgccgg aagatatacg atattccagt tttttactgt ttatggagat
     6781 tcaacgggaa ttgattgatc tggaagatga ggaatattta caaggaatcg aaaaaatcca
     6841 tcaggcaaaa acggtgcaag aacttcagca gctacttctg tcattcatca aaaagcatca
     6901 gagacagaaa aagtacagtg acaatgtgga aaaagtaatc gtaatcctgc accagcatta
     6961 tcaagaacca cttacactca aagaagtgtc agaaaacctg catttaaatg tcatgtatct
     7021 aggacagctg ttcaaaaaag aaacgaaaaa aagtttttca gcttatttga accatttacg
     7081 tatggaaaaa gcgaaacaac tgcttttgca cagtaatcaa aacatcaatg agatagcaag
     7141 tgagattggc tacaacaaca ccacgtattt ctctaaatta ttcaagaaaa tcgtcggcag
     7201 atcccctaaa gagtaccgag aaaacagtgg agaatcataa aagaaaaagc caatgtaatg
     7261 aaataactta taaaaaattg tctagaaatc taattttggg ctgttcttat tacttgaggc
     7321 aaggcagctt tttcatgacc tctcgataaa cggagttcaa aagccagttt ctcggtatta
     7381 agccaaattt atgcaaaaat gtggaaagcc attttcacaa aatttttctt aatactcgaa
     7441 actaaacagc tttttcacga cctctccact aaacggtgtt caatcagctg accctcggta
     7501 ttagctcaaa aaatggcaaa atatgaggaa ctattttgcc atttgtctct ctaatactca
     7561 ggtcagaacg ctgatttcac aacctctcaa taaacggtgt tcaaaagctt gcatctgcgg
     7621 taataagacc aaccaaacga aaaatatgag gatctatttt tgcttggctg ttcttattac
     7681 ttgatgcagg acagcttttt cacaacctct tgttaattat agtttgataa ggaaaaaggc
     7741 aaatcttttg cttgatattt cttagggtga gggatgatcc ctaattggta agagatcgtg
     7801 ccgcctttca gcaaatcttg atgggtaaaa tataatttcg tatattcttt gtccatccgt
     7861 ttgattttat ggataaattg ttgctgcggc cgattaggcg ttgcttcaat cgtcaattcc
     7921 tttccatttg acaaaagcaa aacagccttg tcaattaatg gcataccgat aacatattca
     7981 ccggttccag gcgtgacagg ataaaatccc agactattga agatatacca agcggccata
     8041 cttccattgt cctcatctcc agggtaaccc attggagaat cgtcgaaggt ctgagtcatc
     8101 aactgtttaa gcagtggctg tgccatttca ggttgtccta tataactgaa caagtaagga
     8161 tagtgaaaac ttggctggtt tgaaatagct aattgtccaa attctatcgc ggccatctca
     8221 ctcatttcgt ggatctcaaa accatatcct cctgggtcaa acgttggggg ctgattgcac
     8281 agctcaatga gtttttcttt atatctctca ctgccaccat attttccgat cagtcctgaa
     8341 aaatcgtgat aaacagcaaa gctgctttgc caagcacttc cttctgcata atcttgtccc
     8401 caacgattag ctgaaaatgg ttctctgaac ttgccatctt tatcttttga acgcataaat
     8461 cctgactcat agtcaaagat attttgatag ttcaatgatt gctgctgata tttcttcgtc
     8521 atttcctctt cttctagtac cttggcaact tggctaatac aaaaatcgct ataagcatag
     8581 tctaatgtgt gattgactga ttcgtggtag tgattaggaa catagccata tgtcaagtaa
     8641 tcttctgttc cttggcgtcc ataatttgga tgtttgcttt gaaccgtagc acctttttcc
     8701 attgccgtta gaaattcagg catcaagtct cgtcggatat cttttacagc agcatctgca
     8761 atgaccgcat cgatcaatgt cccaggcata agtccacgtt catcaggaga cagccatttt
     8821 ggcaaatagc cactttctct atagctgttc aaaaaacctt ccagcatttc ttcgtatttt
     8881 tctacagcaa tcaaagaata aagaggataa accgttttat acgtatccca aaacccatta
     8941 tttgtataga gaacaccagg ttttatagat cgactagtcg tatcatagtg gattttttgc
     9001 ccactaggat caatttcata aaatgtttgt ggaaataaaa acacccgata taaattatga
     9061 taaaacgtcc gcaattgttg ttgatcatgg tgtgtgagct gtatacgatc aaaataggac
     9121 tgccattgtt cttgactgtg agcaaggtaa gtctctttgc tttggttttg ttctagttcc
     9181 aggttataag ctgcttgttc cgagctgata aaagatgtcc caaatcggat agtttgtttt
     9241 tttgtctgtt caaaatacaa cgggatcgag tcgctatttt tgttggaaac agctaactca
     9301 tcaattgtta aaggatattc aaatctcaat ataaaataaa aagaaaaatc aggatcttct
     9361 gaaccagcaa aatgtgtgat ttttccttcc actgtgtaag gatctataac aaagagctga
     9421 tggcgtcccg gaaaggataa aagtaaaccg ctgtcatttt tgctgtaatc aatagataag
     9481 attccgccat acatgctagg tatcaatgta gaacagatgc cataacgtaa ttgacagatc
     9541 gacagatgag tcgggcaaaa tatactttct tctgggcggt aagaactttg agcatggaaa
     9601 agggtgttct cctgtagttt tccattgatc ggcgtaaaca gcatatgact gaaatcaccc
     9661 atccaaggac ttggttgatg ggtaagacgg tagccttgaa aaacgcggtc atcaggatga
     9721 aaccaccagc tgccgttttg atccgtagtt tgcggtgcaa aataattcat cccaaaagga
     9781 acgccagtat aaggctggca gttcccatta gagaaatttg gctgattcgc tgttgcatgt
     9841 ctagtatcta tttgaccgat attcatttta tttctccttt gttgattgtc ttttgatgaa
     9901 tgaaactgga aatttactat cggatggtcg atcttttgtc tctaaccaag tgaccaaagc
     9961 atctcccgca agcttaccta actgttcaaa atcttgtgcg atggttgtca gtggcgggtc
    10021 aacaagagct gctgcttgga tatcatcaaa tccgataatt gaaaaatctt cagggatatg
    10081 ataaccatgc tgtctcaaga gattcatggt ctggatagcg acaacatcgt tttcacaaac
    10141 aaaagcagtt acttcatact tagtgatata agaaagcaat tcatctaatg tcgcgctttt
    10201 gtcatccagt gaagtgtgga aagtcaaacc agctttgtcc aatgcttcaa ggtaaccaag
    10261 gtaacgctgt cgaacggatt gtggatgagt ctgcgtacca aacaaataag cgatttttga
    10321 atgtccctgt tcgatcaaat aagaggcagc aagcgcaccg ccttggaaat tatcggaaag
    10381 aacggtagga aaaggtaact catagatttt tttatctaat atgacgacag ggaattcttt
    10441 caatgaaagc tcaaaaagta agtcaagatg ttgttctgta tcaagagcat aataaatcaa
    10501 tccgtcaaat tcattgatta gctcttttgc tgttttattt tgtagaaagt caagcgtcgt
    10561 catcatgaca tcaatctgtt tatcttgcat cacagggttc aatcccttgc taaaatcacc
    10621 aacagacaaa tcagcgacat atggaagaag aaacagaatc cgtcgtgttt ttgtaggaga
    10681 agcaagaatc tgttccggag aagcggattt aacgaaactg cctttcccgc gaacgcgata
    10741 aataagtcct aattgctcta attcagtcaa tgcacgtttg gaagtgattc tgcttacttt
    10801 ataggtacgt gaaagttctt tttcagttgg tagttggctg tctacaggta attcaccagt
    10861 taaaatagcc gtttgcaaat cattaaatat tgtctggtac aatggttgat tcatataaac
    10921 acctcttaat gatatatcat attcaaataa taccataatg gatgaaaaag taaataaaaa
    10981 gatatttttt gaaagcgtag acataattgg ctgtatgaag tataatcttt aaagatatat
    11041 caaatagaaa gaaggtttta ttagtggctt ataaagatat accaaaatcg gtgcagaaat
    11101 ttatggaaga tatcacacaa aaatgtgggg atatccatgc ggactgggca aagaatttta
    11161 cagcagcatt tgccaatact ctgttgacaa cagtcaaaaa gcaaaatgac ggaaccacct
    11221 ttttattgac gggagacatt ccagcgatgt ggttaagaga ctctactgca caagttcgtc
    11281 cttatttagt gattgcaaaa gaagatgagg aattagctga catgatcagc gggttagtca
    11341 aaaagcaatt tttttatatc aatatcgatc catatgcgaa tgccttcaac gaagaagcta
    11401 acggtgctgg ccaccaaaca gatcatacaa aaatgaacga ttggatctgg gaaagaaaat
    11461 atgaaattga ttctttatgc tacccaatac agttggccta tcttctttac aaaaatacag
    11521 gaaaaactga tcaattcaat caatctttta ttgaaggtat caaaaaaatc ttggacgtgt
    11581 tcaaaacaga gcaagatcat gaacactctc cttatacatt tgtacgagac acgacaagaa
    11641 aagaagatac gctgatcaat gacgggcgag gtgcaaaaac agcttttaca ggaatgacat
    11701 ggtctggttt ccgtcctagc gatgatactt gccgttatgg atatctggta ccatccaata
    11761 tgtttgcggt ggtcgtgtta ggttatgtgg aagaaatttt tcaaacgata ctcgatgata
    11821 aagaaattgt acagcaaaca cagaaattga agaaagagat ccaagagggg attcagaatc
    11881 acggacgaac ggttaatcaa aaagggcaaa cgatttatgc atatgaagta gatggaatgg
    11941 gaaacgccac agtcatggat gacagtaatg tgcccaatct gatcgccgca ccttacttgg
    12001 gatattgttc tgaacaagag gaagattatc tgcaaacaag aagaacattg ttgagtaaag
    12061 aaaatccata tttctatgaa ggaaagtacg caaaaggaat tggatcctcc catacgccag
    12121 aaaaatacgt ttggccgatt gcattagcta tggaaggaat gacaacgtca gacaaatctg
    12181 aaaaagagcg gatattgaat ttacttgtag caactgacgc aggaacacac cttatgcacg
    12241 aaggattcga tgtgaacaat cctgaaaatt atacgagaga atggttttct tgggcaaata
    12301 tgatgttttg cgaattagtc atggattact ttgatattag agtagaatag taggaggtat
    12361 cgtaaatgaa aaagaaagta tatatcattt ctcatagcca ctgggatcgt gaatggtaca
    12421 tggcttacga acagcatcac atgcgactga ttgaattgat agacgatcta ctagagctat
    12481 ttgaagttga tccgtccttc aacagttttc atttggatgg gcaaacgatt attttagatg
    12541 actatcttca agtaaggcca gaaaaaagac aggcaatcca acaagcaatc aatgaaggaa
    12601 aattgagaat tggaccattt tatatcttgc aagatgattt tcttatcagc tcggaatcga
    12661 acgtgagaaa tatgctgatt ggcatggaag aaagccgtaa atggggaacc cctgtcatgt
    12721 taggctattt tcccgataca tttggaaata tgggacagac gcctcaatta atgaaacaag
    12781 ctggtatttc agcagcagcg tttggccgtg gtgtaaaacc aattggcttt gataatcaag
    12841 ttttggaagc agaaaactat tcttcccaat actcagagat gtggtggaaa ggacctgatc
    12901 aaacagcaat ctttggtctg ctatttgcca attggtatag caacgggaat gagattccag
    12961 tagaaaaaga agcagctctt gccttttgga agcaaaaatt agcggatgca gaacaatatg
    13021 cctctacgaa ccacttattg atgatgaatg gtgttgatca tcaaccggta caaaaagata
    13081 tatcaaaagc aatccatctc gcaaatgaac tgtttccaga ttacgagttt attcattcta
    13141 attttactga ctatctggaa gcagttcaaa aagatgtgcc agaagactta ggctcagtag
    13201 aaggagaatt gacaagtcaa gaaacggatg gctggtatac tttagccaat acagcatctg
    13261 ctcgggttta cttaaaacaa tggaatacca aggtccagcg acagctagaa aatattacag
    13321 aaccacttgc gacgatggct tatgaagtta gtggaaacta tccccatgat caattagatt
    13381 atgcttggaa gacattaatg caaaatcatc ctcacgacag tatttgcggt tgttcagtcg
    13441 attctgtcca tcgggaaatg atacctcgtt ttgaaaaagc agatgaagtt ggaaagtatc
    13501 ttgcacaaga ttccttagaa cagctgaccg cagcaatcga tacgaccgga ttcccaaaag
    13561 acagctttcc atttgtcatt gtaaatactg caggaatgga taaaactggt gaagcagaaa
    13621 taaccatcga attagaaaga aaaagattcg ctgaaggaat acctgaacaa ctatatcaag
    13681 agttagaaaa tttgcctaaa cgtaagtatc atgtagaaac aaaaagtggg gccactatac
    13741 cagctatttt atctgaagaa actgtccaat ttggttatga tttgccgaaa gaccgtttcc
    13801 gtgtgccata tatggcaaga atgatcaaag tgactttacc attagaaaac atgccagcat
    13861 tttcttggga aacattcgcg cttgtagaag gggaagcgaa agcagaagaa aaggaaacaa
    13921 tgattcatca gagtggacga atcatcgaaa acggccctct acatttgacg attgaaaaaa
    13981 atggtaccat tacaatggaa gatcgcaaaa acaaacgaaa gttgaataat ctgcatattt
    14041 ttgaagatat cggcgatata ggaaatgagt atatctttaa acaacctttc tgtgatcagc
    14101 caatcctatc cagtaacaaa gaaaactctg aagtaaaagt attagtagat acaccggaaa
    14161 tagcaaaaat aagtattcta caggaaatgg aaatccctgt ttctgcagac gagcgtttag
    14221 aaaaagaaca gcagatggtt gtggaatttc gttatcgaaa agcagaacga tccaaagaaa
    14281 aacgaatcct tcaaataaaa acaatcatga caatcagaaa agatagtaaa aaaatcgact
    14341 ttgaaacgac tctcgacaat cagatgaaag atcatcgctt acgtgtcttg tttcctacaa
    14401 agttacatgt ggaacatcac gaagcagata gtatttttga agtggtgaag cgcccaaacc
    14461 atgtgtctaa aagttgggaa aaccccacga atccgcaaca tcagcaagca tttgtcaata
    14521 ttcatgatga agagtacggt gtaacagtcg gaaactttgg tttgaatgaa tatgaagtaa
    14581 cagaagacgg acaaatcgca gtgacgctac ttcgcagtgt gggagaacta ggcgattggg
    14641 gatattttcc aacaccagaa gcacagtgtc taggtgagca tcggttcaat tacagcattg
    14701 aactacatgg accagaagaa aaattctcta cttatttgca tgcatacgca gcacagattc
    14761 ctttttccac tcaacaaatc aaacatcatg aaggaacatt gatttcaaaa caacaatatt
    14821 taacgataaa aagtgaaacg tttgcaatta cagctttgaa acgcagcaag tttagtgata
    14881 aagtagtagt aagaggtttc aatatgtcta gtcatttaga aaaattggaa ataactaaag
    14941 acaatggaaa aactgtcata ttgaatctgc ttgaagaacc aacgaagcaa gcggttgtcc
    15001 caataatcca accttatgag attcggacaa tcggattcga ggaggaaaac gaatgtatgg
    15061 cggaatagaa gcaggcggaa caaaatttgt gtgtgcggtt tcagatggtg gagaaatttt
    15121 agaaaaatta agtatcccta ccacaacacc agaagaaaca ttggatcagg tatttgaatt
    15181 ttttgatcgt tatgaattga agggaatcgg gattggctca ttcggcccga ttggtatcga
    15241 caaaacaaaa gataattacg gatacgtttt ggcaactccc aaaaaagggt gggcacaatt
    15301 cgattttctt ggcacaatca aggaacgcta caatattcct gctgcttgga caacggatgt
    15361 caatgctgct gcatatgggg aattgaagca aggagcagca caaggaaaaa atagttgtat
    15421 ttaccttacc gtgggaacag gaatcggtgc aggtgtcgtg atcaatgaag aaattttttc
    15481 agggatcgca catcctgaaa tgggacatat ttgggtgaaa cgccatccag atgaccatta
    15541 tgaaggtact tgtccttatc ataaagattg tttagaagga ttagcagcag gaccgtctat
    15601 tgaggcacgc acagggatca aagggcaaga tctgccgcaa gatcatcctg tttgggctat
    15661 tcaggcttat tatattgctc aagcactgat caactatacg ttgacgttag cacctgaaaa
    15721 gattatttta ggcggtgggg taatgaatca ggatcatctc cttcaaaaaa tccgtcagca
    15781 atttgttgaa ttgatgggtg gttatatgga aacacctcaa gtaagtgaat atatcgttcg
    15841 ttggggactg ccgaatgaaa gtgggattat cggtagtttg ctgttagcag aacacgaaca
    15901 tcaaaagtaa gcagacaaat tacacaaaaa gaacagagtc ttatcattat ctgctgataa
    15961 gactctgttc ttttttattt ctattcccat aatccaggta aagctttacc gactattttt
    16021 tcttgagtaa atggatggat catgtgcagt tctaatgcat ggagcatcag tcgttcagct
    16081 gttctatttt ggtagagagg gtctccaacg atcggatgtc ccttatgagc taaatgaact
    16141 ctgatctggt gtgtacgtcc ggtttccaaa acacaataga cttgtgtttg ttcagaaagt
    16201 actttgttga cagtgacatg tgtcaaggcg ctctgaccat ttttatcatc tacgaccctt
    16261 tttctccgat catggcgatc tcgtccaatt ttattggtga tggtcatatc tttggcaatt
    16321 tctccccata cgatggcttg atatctccga tagatcattt tttgttccaa cattcttcca
    16381 agtatcggca agacaaatgg attttttgca aataaaacgg caccgcttgt ttctttgtcc
    16441 aagcggtgga caacgtaagg tcgttgctgt ttagtgtcga gataagcagc taaatgattg
    16501 agtaaggtac tgttttctgt cggttcattt ggatgagtct tcattcctgc aggcttatcc
    16561 actataatca aatgctcatc ttcatataaa acacggatgt gcttcgcatc tcctaattga
    16621 acttcttgat aattataatc tgtttcttcg aataacaatg tgatctggtc attgcccttt
    16681 acttcatgat ggaacaaagc aggttgttga ttgatccaaa cgtttttacg tatacgcaaa
    16741 aagtgtcgaa cctttctagg aactaaccat tccttttcaa gcaagtcgcg aattgttgtt
    16801 tcaggatggt tcataggtaa agtgatcgtg attttcacaa gtgtactcct ttcagtgcaa
    16861 tcgcagacta attgtaccat ttttaaaaca tatttaagaa aaaacttaca tttttctgtt
    16921 cattcttatg ttactctact tgcactaacg taaaaaaaca tattgaacta caacttaagc
    16981 aggaagaaaa cactctttca ctatggtaaa ataagacgat tgaatcaaat agacaggaga
    17041 agaagcaaat ggattttcaa agcattttag aaaagataaa agctgctttg atcagttttt
    17101 ggcgttggat cagaccatat ctcggacaat tccatcgatg gcgaaagcgc atctggaaaa
    17161 aatatcatgt gaacaaattg attttgctga ttggtttgat ctgtgtctta gtgacaagta
    17221 tctacctctt tattttagca aaacaagcaa atgtcgaaac gttaaaatca ggattaagcc
    17281 aatcgacagt catttatgat aagaaaaacg aagaagctgg cacactgtac gcccaaaaag
    17341 gaacatatgt agaattaaac gcgatttctc cctatatcca agatgcagta atctcgacag
    17401 aagataggaa tttttacgag catcacgggt ttgatatcaa agggattgcc cgtgcagcag
    17461 tccgaatggt cttgtctggc ggaacatcag ggggcggagg aagtacgatc actcagcagc
    17521 ttgccaaaaa tgcgtatctg acattagacc aaacgtttga tcgaaaagcg aaggaattat
    17581 ttttggcaat cgagatcgaa aagaagtaca gcaaagaaga aatcttgacc atgtatctta
    17641 acaatgccta ttttggcaac ggggtatggg gtgtccaaga cgcttccaga aagtattttg
    17701 gagtagatgc aacgaatgtt acgctgtctg aggctgctac tttggcagga atgctgaaag
    17761 gtcctggtat ttataatccg atcgatcacc caaaaaatgc gaatgatcgg aaaaacacag
    17821 tactaagtgt aatggaagaa aacggaaaaa tcacaaaaga acaagaacaa gcagaaagcc
    17881 agacagatat cagcgcgtat cttaatgaca catacgataa ttcagattct ggttatcgtt
    17941 atccttatta ttttgatgca gtgatcgatg aagcagttaa cgagtatggt cttgatgaag
    18001 aagatgtcat gaataaaggc tataaaatct atacagcttt agatcaggat tatcaaaagc
    18061 aattagaagc tacttatcaa aacagtgcat tattcccagc aaatgcttca gatggtgcca
    18121 tggtccaatc tgcttcagta gctttagatc cgaatacagg tggtgtacaa gcactagtag
    18181 gacgaagagg agatcatgtc tttagaggat tcagctttgc tacacagatg cgtcgttcac
    18241 caggatcaac gatcaaacca ttaagcgtat atacaccagc actggaagct ggatacaagc
    18301 cttccagtat cttgaaagac gaaccgcaaa gttattacga tgcgcataat tttgacggga
    18361 cataccaagg agaagtaccg atgtatcaag ctgttgcaca aagtttgaat ttgcctactg
    18421 tatggctttt gcatgagatc ggtttacaaa aaggatatga taaagcagaa gaattcggcc
    18481 tgcctttagc aaaatctgat aagtattatg gcttagccct tggtggactg gaaaaaggag
    18541 tatctccatt gatcatggcg ggtgcttaca gtgcatttgc caatgatggt cagatgtata
    18601 cgccgcatct tatcacgaaa atcgttgatt cgactggagc ggtgatcgtc gataacacaa
    18661 acaaaaaacc aaaacaggtc atatcaaagg aaacagccga tgaaatgacc agcatgcttc
    18721 ttggtacatt ttcaaatgga acagcggctg ctgcgaatcc tgcaggttat acgatagcag
    18781 ggaaaacagg aaccacagaa actaatttcg atgccacaaa agcaaatgac caatggatga
    18841 tcggttacac gccagacgta gtgatttcga catggatggg ttacgaagaa tccagcaagc
    18901 tgcactattt ggaagggaca agtggaaatg ttgtcgggaa agtcttcaaa tcagcggcag
    18961 aaggcatcct tccgtatact tcaaaaacag gatttagtgt agcagatgcc tatgccacag
    19021 gaggacaagt ggtagctgca gatcaagttg gaaacacagg aaattctaat ggagagtctg
    19081 gaaattggca agataacttg aatcaatatg gtgaacaagc aaaagaaggg ctgaaaaact
    19141 tcggcgatat ggtgaaagaa ggcgttcagg gaattggtga agcggctaaa gacctctggc
    19201 ggaaatatca aggagagtag cccctttgaa aacgaataac cccatgttac aatagagagt
    19261 aactactaaa agaaaagagg gcataacatg tcaaacatct acgatagcgc gaatcaaatc
    19321 gaacgtgaga tccgtgaatt accagaattc aaagcgttga aagaagcgta cgagaaagta
    19381 caagcaaacg aagaagcaca caaactgttc aaagacttcc aagcgatgca aatcgaatta
    19441 caagaaaaac aaatgagtgg acaagagttc agcgacgaag atgcaatgaa agcacaagaa
    19501 atggctgtga aaatccaatc agaagaagta atcaatgact tgatgcaaaa agagcaaggc
    19561 ttcagtacaa tcatcaatga cttgaaccgt atcatcatga caccagttcg cgacctttac
    19621 agcgaataaa actattttat gaaacaacag agggtgtgaa agagacgttc ggccttgagc
    19681 gatgacaaaa ttgcttctta ttgccgatag gctgtctctt gaacaccatt taccaagagg
    19741 tcgtgagcct accgtttaac tctgagttac aaggaacaat ttcaaaaaac agcttctcat
    19801 gtttgatgac tattgtgact tgtgaccgag gagttggcag gagaacaccg tttattcaga
    19861 aataggagct acgacaaaag tcttgtcata gctcct
//
LOCUS       DAZUMK010000044        19529 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000044 DAZUMK010000000
VERSION     DAZUMK010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.044"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..411
                     /locus_tag="ACG808_001477"
     CDS             <1..411
                     /locus_tag="ACG808_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFS5047107.1"
                     /translation="GLYYSPVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAP
                     QEYLSRAYNDAPKYCANIVNTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLA
                     ELKGYQVTTGEPVCQTRRLNSRIDKVRYMFNKIT"
     gene            509..697
                     /locus_tag="ACG808_001478"
     CDS             509..697
                     /locus_tag="ACG808_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFS5047108.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(755..1108)
                     /locus_tag="ACG808_001479"
     CDS             complement(755..1108)
                     /locus_tag="ACG808_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5047109.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            1639..2061
                     /locus_tag="ACG808_001480"
     CDS             1639..2061
                     /locus_tag="ACG808_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS5047110.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2058..2288
                     /locus_tag="ACG808_001481"
     CDS             2058..2288
                     /locus_tag="ACG808_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5047111.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            2786..2986
                     /locus_tag="ACG808_001482"
     CDS             2786..2986
                     /locus_tag="ACG808_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HFS5047112.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            3013..4206
                     /locus_tag="ACG808_001483"
     CDS             3013..4206
                     /locus_tag="ACG808_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5047113.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCALTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(4274..5839)
                     /gene="guaA"
                     /locus_tag="ACG808_001484"
     CDS             complement(4274..5839)
                     /gene="guaA"
                     /locus_tag="ACG808_001484"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFS5047114.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(5898..6821)
                     /gene="coaA"
                     /locus_tag="ACG808_001485"
     CDS             complement(5898..6821)
                     /gene="coaA"
                     /locus_tag="ACG808_001485"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFS5047115.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            7017..7622
                     /locus_tag="ACG808_001486"
     CDS             7017..7622
                     /locus_tag="ACG808_001486"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5047116.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            7930..8946
                     /locus_tag="ACG808_001487"
     CDS             7930..8946
                     /locus_tag="ACG808_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5047117.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            9087..10388
                     /locus_tag="ACG808_001488"
     CDS             9087..10388
                     /locus_tag="ACG808_001488"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFS5047118.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            10434..11096
                     /gene="deoC"
                     /locus_tag="ACG808_001489"
     CDS             10434..11096
                     /gene="deoC"
                     /locus_tag="ACG808_001489"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFS5047119.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            11117..11515
                     /locus_tag="ACG808_001490"
     CDS             11117..11515
                     /locus_tag="ACG808_001490"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFS5047120.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            11581..12663
                     /locus_tag="ACG808_001491"
     CDS             11581..12663
                     /locus_tag="ACG808_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS5047121.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            12875..14437
                     /locus_tag="ACG808_001492"
     CDS             12875..14437
                     /locus_tag="ACG808_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5047122.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            14430..15563
                     /locus_tag="ACG808_001493"
     CDS             14430..15563
                     /locus_tag="ACG808_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5047123.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            15563..16519
                     /locus_tag="ACG808_001494"
     CDS             15563..16519
                     /locus_tag="ACG808_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5047124.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            16618..17784
                     /gene="deoB"
                     /locus_tag="ACG808_001495"
     CDS             16618..17784
                     /gene="deoB"
                     /locus_tag="ACG808_001495"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFS5047125.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            17808..18626
                     /locus_tag="ACG808_001496"
     CDS             17808..18626
                     /locus_tag="ACG808_001496"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFS5047126.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            18643..19350
                     /gene="deoD"
                     /locus_tag="ACG808_001497"
     CDS             18643..19350
                     /gene="deoD"
                     /locus_tag="ACG808_001497"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFS5047127.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT         6243 a         3524 c         4199 g         5563 t
ORIGIN      
        1 ggtttatact atagccctgt tagtactcca gcagattttc ggatgcttac tcctattgta
       61 ctggagcaag cctttagaaa agctggaaca gaattgttag agccatatct tagttttaaa
      121 gtttatgcac cacaggaata tctttcacgg gcatataacg atgctcccaa atattgtgca
      181 aatatcgtaa atactcaact gaaaaataat gaggtcatta ttattggaga aattcctgct
      241 cgatgtattc aagattatcg caatgattta acttttttta caaatgggct tagtgtttgt
      301 ttagcagagc taaaaggata tcaggttacc actggcgaac ctgtttgcca gacccgtcgt
      361 ctaaatagtc ggatagataa agtaagatat atgttcaata aaataactta gtgcgtttta
      421 tgttgttata taaatatggt ttcttattaa ataagatgaa atattcttta atatagattt
      481 gaattaaagt ggaaaggagg agattgttat tataaactac aagtggatat tgtgtcctat
      541 ttgtggaaat aaaacaagac tacgaatacg agtggatact atacttaaaa atttcccttt
      601 atactgcccc aaatgtaaga acgaaacttt aattaatgtt caaaaaatga atataataac
      661 aatcaaagag ccagacgcca agacgcagag ccgataattt gagaaatgaa actctcatct
      721 tatcggctct ttttgtttat ctgaatttta ctgactagcc ttcaatattt cttgcttcat
      781 tgataccttt tgctagtgct tccattaagg atagttcttt gtctgtaaag ctatccatgt
      841 atttttctac ctgtaacctt cgagtgcttt taatcaaatt atctgtaggt aagaaaaatt
      901 catcaataga tacatggagt aatgatacaa gatcatacag cacttgtgta cttgggtgtt
      961 gtcctttgtt ttctatattt gttaaataac gtgggtcaat ttcaatcatt gttcccactt
     1021 gttcacgagt gagaccccgt ttcattcgag cctctttaat cgctaaacca acggctctaa
     1081 aatcgtattt atcttctttt ttacgcatac taaatcacct ctatacattt tactgttcct
     1141 atataaccat aaacaggtat agaaaaacat gttatatagt ttatgagttc ctatagaaca
     1201 ctgtggaatt tattaatgcg atgaatccaa tttcaaatag aaaggtcgct gattttattt
     1261 acctatacaa ctttataaaa tttagtcata ctcctagaaa gtagataaaa ttggcgactt
     1321 aatggaggga aaagtcgcca tgagaaagat aatctgtcat ctagttctct taattaagtt
     1381 cttatcaaaa aaacttatcc acctgtactg gatagatatg accatcaatg tggttttgca
     1441 gattacatag atagaaaaat caaagcatat gaacagagaa aacaatctgt ttatgtgctt
     1501 ttttggtcac ttaataaata tcttttagga tttctcttca tttatgcaac aaatcccccg
     1561 ttcgtgtcgg ggtttagtat gaagggagat aaagaagcac atgccactcc ctttcacgac
     1621 agaaaggggg tgaaaagcat gaaaccatct tcttttcaag aaaccataga aaatcagttt
     1681 gactatatct gcaaacaagt tattgaagat gaacgaaaag attacttcaa acatctagac
     1741 agactatcag caaaagaagt ctcgttttct gatatagata actaccttgt caactctttt
     1801 tctactgttg atacgtacgt cactgatttc caactattta aattatttgg attaagaatc
     1861 aatgttgaaa gtgatttatt aagtgaagcc ctgcgtcatc tttcagagaa aaaacggaac
     1921 attattttac ttcattactt tatggatatg agtgatgtgg agattgcgga gttactaaat
     1981 ttgaatcgtt cgactgttta tcgtcatcga ataagtggac tagccatgat taaagaattc
     2041 atgaaggagt gtaaagaatg aaaacagctt atccaagggt tcccttcccc ctcattgtaa
     2101 aggcgacaga tggcgacgtg gaagcaatta atcagattgt taaacattat cgagggtata
     2161 cctcaaaacg ttcattacgt cgcatgacag acgaatatgg aaattctcat atggttattg
     2221 atgaaaccct acgtggcaga atggaaacga aactcatctc taagatttta tcgttcgaaa
     2281 ttaaatagtc ccattttttc gtggaagcgt gtcgaatcgt atccacgctt cccgatttgg
     2341 gaggtttgtt attccatcaa agcatattgt gtgttcagta ggttttgata ggctaacaag
     2401 ccaaacgttc tttgacaacc aaataaaggc aatcgaatac gtttcggtaa gaaaagagct
     2461 gatgaactaa cacgccatga cctacaaaat tagtgagcga ttcatgttag taattcgaaa
     2521 gtaatcttta gcaggattgc tgccatgact ttcttatcgt gataatgata ctcccgtata
     2581 gccatagtcc gagcgttaga agcgtcacag gcaatgggta cggctacatg agaaccatgc
     2641 agaagtgaaa ttcctgtgag ctttgctaga gctgttcgat tgctaagata ttcttctctt
     2701 gtcagtgtat tattataaaa ctatttgata taattatagg tgaatattgg agttttccta
     2761 gcctataatt gggaaaggag taataatgat aacggtcaca aaaaaggatt tagttgagct
     2821 aggatatgga acttcttttt cagcagacat cataaaaaaa gcaaaagaat tgatgattac
     2881 aaaagggcat atctactatc aatctcgaaa gctagataga gtccctcgtg aagctgttga
     2941 agaaattcta ggaattcggt tttcaaatga aaacaggtct gagtagtttt tgcacagtaa
     3001 ggagtaaaaa ctatggttaa agacccaata aaaaaagcaa agaatggaac ctattatttt
     3061 cgtgcaaatc taggttatga ttctaaaggg aaaaaaattc aaaaatatcg tagtggattc
     3121 actactaaaa aagaagcaag agaagaatat tcgaaactgc tattgatgaa accagaagaa
     3181 ttaagtgaga ataaggataa aatgacgttt aaacattata tctttgaaat tttccttcca
     3241 tggtataaga cacaggtaaa gctcagaaca tatgaaaatc gcttaccaac tattaagaag
     3301 catttctctt attttgataa aatggctgtt tccgatattg agcctattga tgtgcaaaat
     3361 tggcaactaa aattatcaaa gaagtgtaag tcttcttatg taagagctgt tcaaggacta
     3421 ttttcagttg ctatggatcg agcaattgtc cttgggataa ccaccacaaa tccatctaag
     3481 attattggca atgtgaaaaa gcaaaagtct aaaattgaat tttggacaaa agaagaattt
     3541 gaaaaagtaa tctctctaat ttacaaagaa gattactatc aacacttttt atttatctct
     3601 ctgtggtttt tatttatgac tggaatgcga attggtgaag ctactgccat tcaatgggaa
     3661 gatattgatt ttgactcagg agtactaacc atcaacaaaa cactctatta caagaatcaa
     3721 tcgaattata gatttgttga accaaaaaca aaggcgagcg ttcgtcatat tgtattagat
     3781 aaatgcgcat taacttattt atccgaatgg aaggaagtac aacaaaatct aattcagaca
     3841 gactttgtaa tgagctataa cggtattcct acccaaaaac atacactatc atatgctatt
     3901 gaacgctatg ctaagctggc tggtatccat cgaatcagaa tccatgggtt acggcattct
     3961 catgcttcct tgttaatcag tatgggagaa aatccactta tcattaaaga tcgtctagga
     4021 cacgaagata ttgagacgac tttaggaacg tatggacatc tttatcctaa cagtaatttc
     4081 gaggtagcta acaaattaaa tggagccatt tcctttaaag aagcaaaaca aaattatgat
     4141 acttcaccaa agaatcaatt tactgtagac tacttgcgaa acaaagaaat gaaaaaagtg
     4201 caataaaagt gcagtaaaaa gataagaaga gccagaaacc cttggtataa gaggatttct
     4261 agctctccta caactattcc cactccactg ttgctggtgg tttagaagtg atgtcataca
     4321 cgatacggtt gacgtgggct acttcgttta cgatacgtac agagattttt tgcaagactt
     4381 cccatgggat acgggcaaag tcagcggtca ttccgtcgat tgaagttaca gcacggatac
     4441 cgactgtgta gtcgtaagta cgtccgtcac ccataacacc gacagagcgg atccctggaa
     4501 gaacagtgaa atattgccag atatcacggt caagtccagc agctgcgatt tcttcacgca
     4561 agatagcatc agattcgcgt acgatttcta gcttgtcttc agtgatttcg ccaagaacac
     4621 gaataccaag accaggacca gggaatggct gacgccaaac gattgaatca ggcatgccta
     4681 actgagtccc taattcacgg acttcgtctt tgaataatgt gtttaatggt tcgatcaatt
     4741 caaattgcat atcttctggc agtccaccta cattatggtg agatttgatc gtctgtgctg
     4801 tttctgtacc agattcgata acgtcagtat acaacgttcc ttgtgctagg aaagagacgc
     4861 cttcttcacc agcaagtttt gttgcttcgt catcaaaaac atatacgaat tcattcccaa
     4921 tgattttacg tttttgttct gggtcagata cacctttcaa cttgtctaag aagcgatctc
     4981 ttgcatcgac tttgatgatg ttcaagccga attttccgcc tagactttcc attacttgtt
     5041 ccgcttcgcc tttgcgtagc agaccgtggt caacaaagat agatgtcaat tggtcaccga
     5101 tcgctttttg taaaagaacc ccaacgacac tagaatcaac gccacctgaa agaccaagca
     5161 agactttttt gtcaccaact tgttcacgga ttttttctac ttgcatatcg ataaagttat
     5221 ccattgtcca gttaccttca cagtgacaaa catcaaaggc aaagtggcgc agcaattcga
     5281 ttccgtattc ggaatgacgt acttctgcat ggaattggat accataaaaa ttacgtgttg
     5341 tgtcttggat agaagcaatc ggacaatctt tgcttgttcc gactgtttca aagccttcag
     5401 gaactttagt taccaagtca ccatggctca tccatactgt ttgttgtttt ggtgtttctt
     5461 taaacaagac agcttgatcg cttgttactt ctaacaaggc tttcccatat tcgcggtttt
     5521 tagcaggctc tacggaaccg ccaagtttgt acgtgatcaa ttgcatacca tagcaaatcc
     5581 ctaatacagg aatacctaaa ttgaagatct caggatcgat cccaaatgca ttctcatcat
     5641 atacactgtt tggtccgcca gaaagaatga ttccctttgg attcatcgct tttacttctt
     5701 ctgcagtaat gcggtggctc aataattcgg aaaagactcc aagatcacga atacgtcttg
     5761 tgatcaattg gttatactga ctaccgtagt ctaatacgat aatcttttca acgttcgtca
     5821 tatcggcaac gtttgtcaaa tttattaccc ctatcccttt aaattttatc tcaaaatagt
     5881 caaactattt taaaatgcta atattttcgc atgaaaatcg aatcgatgat atgattttca
     5941 gttttatgga gaattatatc ggctcttcct cgtgtaggca agatatattc ctgtaaattt
     6001 ttcaaattca catttttcca tacatctttt gccattttaa aggcttcttc acgatctccg
     6061 atagcaaaac gataataata attctctgga ttttggaaag ccgtatccag taacgcacca
     6121 aaacgatcca aataccattt ttcgatcagc acgggatcgg catcgacata gacagaaaaa
     6181 tcaaaaaagt cgctgacgta gatttgctga ttcgcaggta attgaagcgt attgatcccc
     6241 tcaacgatca agatatcagg ctgctggatc aattcgtact tgtctggaat gatatcatac
     6301 acatcatgtg agtaaacagg tacttttatc tcttcctgac cagattttac acggttcaag
     6361 aaatcgatca gcatctccat atcataactt tcaggaaatc ctttacggtc catgattcca
     6421 cgctctttta atatacgatt tggataaaga aatccgtcag tcgtgatcag ctgaacgttt
     6481 cttcgtttga aggtacgaga caaaatcgtc tgcagcagtc tggctgttgt actttttcca
     6541 acggctacac ttccggcgat cccgataata aaaggcggta cttctacgta tttatgaaga
     6601 aataaacctt tactcaagaa caaagattca aattctttca tataaagata aatcagatga
     6661 gcaagaggta catagatatc tcgaacatcc tgcatggaaa tgcggtcatt caaacttttg
     6721 atattatcta gttcatcttg tgtcaaaggt gcttgcccat tacgataaaa ttctcgccat
     6781 tcttctcttg ccacacggta gtagttcatt ggatcattca tattagcctc ctgcgttctc
     6841 ccatcaatgg aactatttta acacaaaata aacgcaaacc ccaccactta agagagattg
     6901 ccgatctttt tgtataatct ttataataga aagaaacgga gtcttattct tgacttgcgc
     6961 acggatacat aaaaggatag actgttgtca tgaaagaaat gagaaggatg gaagacatga
     7021 caaatcatta ctattctgaa cgcccagata cggcgcacga ttttgaacaa tgggcttttg
     7081 agttaaaagg cagaaccttt caatttgtaa cagattctgg tgtattttca cgtgaaacag
     7141 tggattatgg ttcacgtgtc ctgattgatg ctttcgattg ggaagagtta cctgatgaag
     7201 gaaagatctt agatgtggga tgcgggtatg gaccgattgg gttagctttg gcatttgcga
     7261 cacagcggtt tgttgaaatg gtcgacatta atgaacgagc ggtaagtcta gcgcaaggaa
     7321 acgcaaagcg taaccaaatc gaacatgttg atatccatca atcgaatatc tatgaagcag
     7381 ttcatgaaga aacctatgca gctatcgtaa gcaatccgcc gatccgtgct ggaaaaaaag
     7441 ttgtacatga aattttgaca ggcgcatatc ctcggttgaa gaagggcggt acactgacaa
     7501 ttgtgatcca gaagaaacaa ggagcgccga gtgcgcaaaa gaaaatggaa gaaacatttg
     7561 gtaatgcaga aatcgtcaca aaagataaag gatattatat catcaaaagt gtgaaagaat
     7621 aactagctga aaaatagtgg agcgacgaaa gagtggaact gggcgtgcat gtacgggtac
     7681 gtatcagttc tttttttgtt gagaagtttt taaaaacaaa gaactatttt ttcttcaaaa
     7741 agaaatagta ggaacagagt cgttgttttt tacgataaaa taaataagat acagaacttg
     7801 tatagaaggc tgccaagtcg gtagataaag gaggttttta tcaaaaaaca tactttcctt
     7861 ttttctctat tcttttcttg ggggttttgg tacactgata gggaatttag atagaaggga
     7921 gccaatagga tgaagctgac catcagagat atcgcagaaa tggcaggcgt atcagtaaca
     7981 actgtctcac agattttgaa taacaaagga agcagattta gtgataagac aagaaaaaaa
     8041 gtgttgtcga tcgtgaatga acatcattat aagcccgatt atttcgcttc aaatgtgatc
     8101 aatcgacatt caaagaccat cggaatgatc gtccctgatg tcactgattt cttcttctcc
     8161 aaactgatcg aaggtgtaga gagctatttg aatcctttgg gctatgtgat catgttatgc
     8221 aattctcgtc atagccaaga aaatgagatc aagtatttac aagagctttc ccaccgctca
     8281 gtggacggaa tcttgctggc cacaccaaat attttacctg agaaatatgc attagacagc
     8341 ggtttttatc aaaaaatgcc aatcatttta atcgatcgcg gattgaatcg tcgggataac
     8401 ggacggctta tcgtaaagga atatgagggc gcctatcaag ctgtttcgct gtttattgaa
     8461 aatggccata cgaagatcgg catgctgaag gaaaccactg gatactatca attagaagaa
     8521 cgtttcaacg gatatcgtca tgcgctgaaa gatcatggcc tgccttttaa tgggaaattt
     8581 gttgaacatg gagagttgac tgtccaaggt ggatatactg catcaaaaaa attactcaaa
     8641 cataaagata tcacggctat tttttgcggg aatgatgcca tggcgatcgg atgctatcag
     8701 gcgatcgatg aattgggcaa aaaaatccca gaagatatct cagtcatagg attcgatgga
     8761 ttaaagctgt cagaatacat gattccgcaa ttgacgacag tgcagcagcc aagttttgat
     8821 attggatttt atgctgcacg tttcctgatc gatacgatcg aatttcctca aagaaaagtt
     8881 ccgaacaaag tgttcgaaac aaaattaatt attcgtgaga gcgtaaaagt attgacaaag
     8941 ggctaactcg gcggtatatt gcttatgtaa gcatttacaa aggctagtta gtgctttttt
     9001 tatttttagt aaaccagttt actaaagtgg tttacctttc ctaaaaataa tgtataatac
     9061 cgaatgaaga caaggaggat caggcaatgc gaatggtgga tctgattgaa aaaaagaggg
     9121 atggaaacga gctgtcgaaa gaggaaatcg agtacatcgt cacgaactat acgaatggaa
     9181 agattccaga ttatcaagtc agtgcattat tgatggcaat tttttatcaa gacatgacaa
     9241 atgaagaaat cacgaactta acattggcga ttgctaattc tggtgatgtc atcgatttga
     9301 gttctcttga aggaataaag gtagataagc attcgacagg tggcgttggt gacacgacca
     9361 cattgatttt agccccccta gtagctagtg tcggagtgac agtggctaaa atgtctggac
     9421 gcggtctagg ttacacaggt ggtacactag acaagctaga agcaattccc ggatttcaga
     9481 tcgagctttc ggatgaagaa tttgtacgca ttgttaatga aagtaaagta gcggtaattg
     9541 gtcaatcagg aaacttggca ccggcggaca aaaaattgta tgccttgcgt gacgtcacag
     9601 cgaccgttga ttctcttccg ctgattgcca gttcgattat gagtaaaaaa atcgcggcag
     9661 gagcagatgc aatcgttttg gatgtgacca ctggagacgg tgctttcatg aagaacatcg
     9721 aagatgcaag acgtctggca aagaccatga cgagcattgg gaaactggct aaccgagaaa
     9781 cagtggcggt tatctcagat atgtcagaac ctcttggcga agctatcgga aatagtctag
     9841 aagttgtcga ggcaatcgaa acattacaag gaaatggtcc agaagatcta gtagaaatgt
     9901 gctacgcctt aggaagccaa atggttgttc ttgctggcaa agctaagaca accgatgaag
     9961 caagaacgtt gcttcaagaa gctctggaat caggtaaagc gttagcaaaa ttcaaagaaa
    10021 tgatccaaaa tcaaggggga gacccagcga tcgtggaaca tccagaacga attttgactg
    10081 cacgctacac aatggagctt cctgctaaac aaagcggagt tgtgtcaaaa atcgtggcaa
    10141 atgaattagg aattgctgca atgatgctag gtgctgggcg taaaaccaaa gaagaagata
    10201 ttgatcacgc tgttggattg aaactgcata aaaaaattgg tgacactgtg acaaaaggtg
    10261 agtcactatt gacgatctat agtaacgacg aagaaatcac ttctgtcatt gaattattat
    10321 ataaaaatat tgaaattggt gaatcggcaa tgaagccaac gttgattcat gacatcatca
    10381 cagaataaag gaaaaaagcg gactttaccg ttttaaagaa aaggggaaaa gaaatggaac
    10441 taaaccgaat gatcgaccat actattttga aagcagacgc atctaaagaa gctgtgatgc
    10501 aaattatcga agaagcaaaa aaataccatt tttattctgt ctgcatcaat ccagcatggg
    10561 ttgcgctagc gaaagaacaa ttacaaggtg agccagtagc cgtatgtaca gtaatcgggt
    10621 ttcctctagg agcaaacaca cccgagacaa aagcatttga aacaacagat gccatcaata
    10681 acggtgcaga tgaagtcgat atggtcatca atatcggtgc tttgaaatca aaggatgatc
    10741 aacaagtcca aaaagatatc gaagcagttg ttgaagcagc aaaagatcgt gcgcttgtca
    10801 aagttattat tgaaacatcg ttattagata gagaagaaat catccgagct tgcgaaatag
    10861 caaaagctgc aggcgctgat tttgtcaaaa catcaactgg tttctcaaca ggcggagcaa
    10921 aggtagagga tgtccgtttg atgcgtgaaa ctgtaggacc agaaatgggt gtcaaagctt
    10981 ccggcggtat ccataacgaa gaagaagcaa tggcaatgat cgaagctggt gctacaagaa
    11041 tcggaacaag cgcaggagta gcaatcgtat ctggttcaac tggtgaaggc tattaatttt
    11101 ttcaaaggag aagctcatgg cagaactcaa acaagaatgg atcaacacag ctattgaagc
    11161 gttagataaa gcctatgttc cttattccca ttttccggta ggcgcttgtc tcgtaacgga
    11221 atcaggcaag atttatcaag gcatcaacat tgagaatgca tcatttggat taacgaattg
    11281 tgccgagcgc acagcttttt tcaaagcagt atctgaagga gaaaaaacat ttacgcatct
    11341 agtcgtagcg gggcacaccc ccgatcctat ctctccttgt ggtgcatgtc gacaagtaat
    11401 ggcagaattc tgttcccctg atatgccggt tacgctagta ggagacaatg gtgtgacgaa
    11461 ggcaacgacc gttcgtgcac tgctgcctta tgcatttact gaaaaagatc tataactcat
    11521 gttcattcag tcagctgact gttgaaaaat aaaacaatta ttttaggagg ctttacagaa
    11581 atgaaaaaag caaaattatt tggtttaggt gctgtcgcac tagcagcagg gttattttta
    11641 ggtgcatgcg gcaacaacgg ctctactgat tcatccggtg gcaaaagctc atcagacaca
    11701 acaactgcag cattgatcac agacacaggt ggtgtagacg accgttcatt caaccagtca
    11761 gcttgggaag gactagaaaa atgggggaaa gatcaaggac tttcacgagg aaatgatggc
    11821 ttccaatatt tccaatcttc aaatgaatca gattacatcc caaatattga ccaagcattg
    11881 aatgctggat tcaaaacgat ttttggtatt ggttataaac taaaaccagc gatccaagaa
    11941 caagcaacaa acaatacagg tacgaacttt gttattatcg atgatgtcat cgatggatta
    12001 gataacgttg tatctgcaac tttcaaagac aacgaagctt cttatcttgc aggtgttgca
    12061 gcagcttata cgactgaaac aaacgtagtc ggctttatcg gcggtgtaaa aggtgaagta
    12121 atcgatcgtt ttgatgctgg attcaaagcc ggtgttgatg caggtgcgaa agaattagga
    12181 aaagaaatca aagtattgaa tcaatatgct ggtgacttct ctgctccaga taaaggacgt
    12241 tcaattgctc aaggtatgta tgcacaaaac gcagatatca tcttccatgc ttcaggcggt
    12301 acaggtaacg gtgtattcca agaagcaaaa tcattgaacg aatcaggcga caaaaaagtt
    12361 tgggttatcg gtgttgaccg cgaccaatca gacgaaggcg aatacacatt aaacggcgaa
    12421 aagaaaaact tcacattgac atctacactg aaagcagttg gaacagtagt agaagatttg
    12481 gcacaaaaat cagcggacgg taaattccct ggcggcgaac acactgttta tggattgaaa
    12541 gaagacggtg ttggtctaac agaaggacaa ctttctgacg aagcgaaaaa agcagttgat
    12601 gaagcaaaag aaaaaatcat ttcaggtgac gtaaaagttc cagaaactcc tgaagaaaac
    12661 taaaaccatt tctatagcct tgttagacac atgtttgaca aggctatggt ttgattagaa
    12721 caagaattgt tcatttatcc aagctgcctt gtgacagttt ggataaggga acaactccct
    12781 gaagtaatgt ttgtttcaaa agaagaacgc tcactaagcg tgaatcttag tcaatcgttg
    12841 ccctagatag aaatcgttag gatgtgaaaa aaaggtggtt caagaaaata ttgtcataga
    12901 aatgcagaat attaccaaag aatttggaac attcaaggca aatgacaaca tcaatttaca
    12961 agtaaaggca ggagaaattc atgcgttgct tggagaaaac ggtgctggca aatctacatt
    13021 gatgaacgtg ctttccggat tattagagcc gacatcaggg aaaattttga tgcgtgggaa
    13081 agaagtaaag atcacaagtc cgaccaaagc caaccaatta gggattggga tggtccatca
    13141 gcactttatg cttgttgatg cctttactgt aacagaaaac atcgtgttgg gaagcgagcc
    13201 tagtcgcgca gggatgcttg accataaaaa agcacgaaaa gagatccaaa aagtttctga
    13261 acagtatgga ttgtcagtca acccagatgc ttacgttcgt gatatttcag ttggaatgga
    13321 acaacgggta gaaattttaa aaacacttta ccgaggagca gatgtactga tttttgatga
    13381 gccgacagct gtattgaccc ctcaagaaat tgatgaatta atcgtgatca tgaaggaatt
    13441 agtcaaagaa ggcaagtcga tcattttgat tacgcataag ttagatgaaa tcaaagcagt
    13501 agctgaccgc tgtacagtta tccgccgtgg aaaaggaatc ggtacagtca acgttaaaga
    13561 cgtaacctca caacaattag ccgatatgat ggttggaaga acggtttcat tcaaaacgat
    13621 gaaaaaagaa gcgaagcctc aagaagtcgt tttgtctatt gaaaatctag tggtgaaaga
    13681 aaatcgtgga ttagaagccg tgaaaaactt gaacttagag gttcgtgctg gcgaagtact
    13741 tggtatcgct ggaatcgatg gaaacgggca gtcggagttg atccaagctt tgactggttt
    13801 gcgaaaggca gaaagcggac atatcaagct aaaaggggaa gacatcacta ataaaaaacc
    13861 ccgaaaaatc actgaacatg gtgtaggaca cgtgccagaa gaccgtcata aatacgggtt
    13921 agtcctagat atgacattat ctgaaaatat cgcactacaa acctatcatc aaaaacctta
    13981 cagtaaaaac ggcatgctga attattcagt aatgaatgaa catgccagag aattgatcga
    14041 agaatatgat gttcgaacaa cgaatgaact tgttcccgca aaagctttat caggcggaaa
    14101 tcagcaaaaa gcaattatcg ctcgtgaagt cgaccgagat cctgatctgt tgattgttgc
    14161 aaatccaact cgtggactgg atgtaggtgc gatcgaattt attcataaac gtctgatcga
    14221 acaaagggac aaatacaaag cagtgttact gatcagtttc gaattagaag aaattttaaa
    14281 tgtttcggat cgtattgctg ttatccatga aggagaaatt gtcggtatcg ttgatccgaa
    14341 agaaacatct gaaaatgaat taggtctatt gatggcgggc tataccttag aagaagcacg
    14401 aaaagagtta gaaaaggggg ctggcgcaca tgaatgatcg cagcgaaaaa ttccgtaatt
    14461 tattagtgcc tgtactttca gttgttttag gattgatctt aggcgcgata ttgatggctg
    14521 cttttggttt tcagcccatc caaggttatt cttctatgct agatgcagcc cttggttcgc
    14581 aaagagggat aggtgaaacg ttaaggcaag caacgccgtt gatttttaca gcattaggtt
    14641 tttctgtagc taactcagct gggtttttca atattgggct ttctggacaa gcactttgcg
    14701 ggtggatcgt cagtgtctgg gtggcacttg cgtttccaga tctgccaaaa gttgttttgc
    14761 ttccgttatg cgtgatctta ggtgccttag ctggtgcgat ggcagcggct gttccaggtt
    14821 tacttcgagc attttttgga acaagtgaag tgatcgtaac aatcatgatg aactatatct
    14881 tattatacgt aagcacattt gctttacatg aagtgatgcc tgaaagctat cgctcaagtt
    14941 tggactcttc caatatgatc agtcaaaatg cctcactgcg gttaccatgg ttaacacaag
    15001 cttttggcgg ctctcgcgta aactcaggat tatttttagc aatcatcgcg cttattttcg
    15061 tttggctgct gatgaagaaa acgactttag gatttgaaat ccgttctgtt ggattgaatc
    15121 cttttgcttc tgagtatgca ggaatgagta gtaaacgtac gatcgttttg tcgatggtca
    15181 tttctggcgc tttggcaggg cttggcggtg tggttgaagg attaggaacg tatcaaaact
    15241 tctttgttca aacgacttcg ctatctattg gatttgacgg aatggctgtt tccttgcttg
    15301 gttctggatc agcaatcggt attcttttat cagcgatctt gttcagtatt ttgaagatcg
    15361 gtggacttgg catgcagaca ggagcaggtg ttccgtttga gatcgtcaat gtatcgattg
    15421 ctttgattat cttctttgtc gggatcaact tcctgattcg tttcgtcatg gctaaagtct
    15481 tccaagggaa aaaacaagaa gagatcgttg cgacaataga atcgaaacca acgaatcaaa
    15541 accatgaagg gggcgaacta taatgtcgac tatcgaatta attgccttga tccttacttc
    15601 tacactggtt tattcaacac cgctaattct aacgtcacta ggcggaactt tttctgaacg
    15661 aagcgggatc gtaaacgtag ggctagaggg aatcatggtc atgggtgctt ttagttccat
    15721 cgttttcaac ttgacgatga gtccatcact tggttctatg acaccatgga tcgctatttt
    15781 agtgggtggc gtcgtaggta tcctattttc tctgattcac gcagtagcaa cagtcagttt
    15841 gcgtgctgac catattatca gcgggacagt aattaatctg atggcacctg ctttaggtgt
    15901 tttcttgatc aaagcatggt atggtaaagg tcaaactgat aacatcacgc agaatctggg
    15961 gtatttttct ttcccaggat tagcagatat tccggtcatt ggaccaattt tctttaaaaa
    16021 tacattctta cctgcttatt gtgctatttt gattgctgtc ctagcttggg tgatcatctt
    16081 caagacgaaa ttcggcttgc gcctccgttc agttggtgaa aatcctcaag ctgccgacac
    16141 attaggtatc aatgtatatg ctatgcgtta ttcaggcgta ctgatttctg gcttgttagg
    16201 cggtatgggc ggtgctgttt ttgctcagtc gatttccggt aacttttcag ctggtacgat
    16261 cgttggacaa gggttcatct ccatggctgc tatgatcttt ggtaagtgga atcctttagg
    16321 ggcaatgggt gcctcattgt tctttggttt tgctcaaagc ttgagtatca ttggcgcaca
    16381 attaccagtt atttcttcga ttccgccagt attgctgcaa attgctccat atttgctgac
    16441 aatcattgtg ctggttgttt tcttagggaa agcttctggt ccaaaagcaa acgggaagaa
    16501 ctatatcaaa tcaaaataat cttttagcga ataaaaaggg tgggttacgg gaagtgtttt
    16561 tcgtctcgcc ctttttgttt taaaggcaat aaaaataaaa agaaagaagg aataatcatg
    16621 tttaaacgcg tacatctgat cgtcatggat tcagtaggaa ttggagaagc accagatgcc
    16681 gataagttcg gtgatgttgg ttctgataca ttaggacaca ttgcgaaaga agcaggattg
    16741 aacgttccac atttagagca gcttggttta ggaacgatcc gtcctcttga aggtgtggaa
    16801 tcagtgaaag atcatcaagg ctatgcgacg aaattagaag aagtttctgt agggaaagat
    16861 acgatgacag gccactggga aatcatggga ttgaatatca aaactccatt tcgtgtgttt
    16921 cctaatggat tccctgatga tctgatcaaa caaatcgaag aattttctgg ccgtaaagtc
    16981 gtatgtaaca aaccttatag cggaacagct gttattgacg attacggtga acaccaaatg
    17041 aaaacaggcg atttaattgt atatacttct gccgatcctg tccttcagat cgcggcacat
    17101 gaagaaatca ttcctttaga agaattatat cggatttgcc aattcgttcg tgatataaca
    17161 aaagatgatc cgtatatgat tggccgaatc attgcacgtc cgtacgtggg tgaaccagga
    17221 gacttcacac gaacaagcaa tcgccatgat tatgcgttga atccattcgg acacacagtc
    17281 cttgattctt tgaaagaagc aggaaaggac gtgattgctg ttggaaagat caacgatatt
    17341 ttcaacggac aaggaatcac tgaatctgtc cgcacgaaaa gcaatatgga tggcgttgac
    17401 cagctgctga aagttatgaa aaacgatttt tcaggaatca gctttaccaa tttagtagat
    17461 tttgatgcat tatacggtca tcgtcgggat gtagtcggct atgcgcatgc gattgaagaa
    17521 tttgacttac gaattccaga attgcttgaa gcgatggaag aagacgactt gctaatgatt
    17581 acagccgatc acggaaacga tccgacgttt actggtaccg atcatacgcg agaatatgta
    17641 ccgttattgg tttacagtaa aaaaatgaaa gaacaagggg cattgcctca aggatattat
    17701 tcagatattt cagcaacgat tgctgagaac tttagtgttt cttcgactga aaatggcgaa
    17761 agcttcttga aattattaaa ataatctttt taaataggag aaagcaaatg accaagttaa
    17821 gcgagatgtt acaagaaacg acccagttta tccttgataa aggagtaaaa gaaattgatt
    17881 ttggtttgat tctaggttca ggattaggtg aattagcaga tgaaatcgaa gatgctgtaa
    17941 tgatttctta tgatgagatc ccgttctttc ctgtttcgac agtagttggg catgcaggaa
    18001 agctagttta tgggtctctg gcaggcaaga aagtgttagc catgcaaggg agattccact
    18061 actatgaagg tcattccatg caaacagtga cctatccagt acgtgtgatg gccggactag
    18121 gggcccattc agtcattgtg actaatgcat gtggtggtgt caacgaatca tttgtaccag
    18181 gagatttgat gctgattacc gatcatatca attttactgg tgaaaatcct ttgatcggtc
    18241 cgaatgaaga tgcaatgggc cctagatttc cagatatgag tcaagcttat acgcctgact
    18301 atcaagcaac agcaaaagca gcagcagaaa aactaggttt ggatcttaaa gaaggcgtat
    18361 atatgggcta ttctggacca acttatgaaa cgcctgcgga gatccgaatg tctcggacaa
    18421 taggagccga tgcagtaggg atgtcgactg ttccagaagt catcgtagca gctcacagcg
    18481 gattaaaagt gttaggaatc tcttgtatta ccaacttagc agcaggtatg caagcgaatc
    18541 tgagccatga agaagttgta gaaacaactc agagagtcaa acaatctttt aaagcattgg
    18601 ttaaagaaac attgacatta ttgtaatagg aggaacaata aaatgagtgt gcatattgaa
    18661 gcgaaagaag gcgagatcgc cgataagatt ttacttccgg gagatccttt gcgagctaaa
    18721 tatattgcgg agactttttt ggaagatcca gtttgttaca atcaggtacg cggtatgtta
    18781 ggctttaccg gcaaatataa aggagaacgt atttctgttc aaggaacagg aatgggaatg
    18841 ccatcagcca ccatctatgc ccatgaattg atccagtcgt atggtgtaaa gaaattgatt
    18901 cgagtaggta catgcggtgc attgtcaaaa gacgtccatg ttcgtgattt agtactagca
    18961 caaggtgcag cgaccagttc ttcgatgatc gaaaaaaatt tccaagcctt tcattttcct
    19021 ccaatcagtg atttcaatct attactgaaa gcatatgaga tagctaaaga aaagggctat
    19081 acggttcatg taggaaatgt tctttcggaa gattctttct acaaagatga tttgacagag
    19141 acgttccaat tagctgaact tggcgtttta ggagtggaaa tggaagcagc ggctctttat
    19201 tacttaggcg cgaaatacca tgtgcagaca ttaagtttga tgaccgttag cgaccatttg
    19261 atcaccggtg aagaaacaac agctgcagaa cgtcaatcta cgttcaatga aatgatcgaa
    19321 gtaggattgg aaacagcgat agcagaataa gaaggaaatg aaaaagctgc cctgcattaa
    19381 gtaataagaa catccaagca aaaatagctc ctcatgtttt tgacttggct ggacttatta
    19441 ttgcaaatgt aagcttttga acaccgttta ccaagaggtt gtgaatctgc cgtttaactc
    19501 tgagtcacaa ggaacaattt caaaaaaca
//
LOCUS       DAZUMK010000045        19452 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000045 DAZUMK010000000
VERSION     DAZUMK010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.045"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..3660
                     /locus_tag="ACG808_001498"
     CDS             133..3660
                     /locus_tag="ACG808_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFS5047128.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="ACG808_001499"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="ACG808_001499"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFS5047129.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="ACG808_001500"
     CDS             7448..7789
                     /locus_tag="ACG808_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFS5047130.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="ACG808_001501"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="ACG808_001501"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFS5047131.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIVRKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /gene="pheT"
                     /locus_tag="ACG808_001502"
     CDS             9146..11566
                     /gene="pheT"
                     /locus_tag="ACG808_001502"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFS5047132.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACG808_001503"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACG808_001503"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFS5047133.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="ACG808_001504"
     CDS             13000..13737
                     /locus_tag="ACG808_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS5047134.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="ACG808_001505"
     CDS             13751..14566
                     /locus_tag="ACG808_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFS5047135.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="ACG808_001506"
     CDS             14579..15226
                     /locus_tag="ACG808_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS5047136.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="ACG808_001507"
     CDS             15242..15898
                     /locus_tag="ACG808_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS5047137.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="ACG808_001508"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="ACG808_001508"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFS5047138.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="ACG808_001509"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="ACG808_001509"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFS5047139.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="ACG808_001510"
     CDS             18240..18758
                     /locus_tag="ACG808_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS5047140.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /gene="cbpB"
                     /locus_tag="ACG808_001511"
     CDS             18865..19365
                     /gene="cbpB"
                     /locus_tag="ACG808_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFS5047141.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6537 a         3340 c         4177 g         5398 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg tgcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga ca
//
LOCUS       DAZUMK010000046        19282 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000046 DAZUMK010000000
VERSION     DAZUMK010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.046"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            74..1015
                     /gene="opp3b"
                     /locus_tag="ACG808_001512"
     CDS             74..1015
                     /gene="opp3b"
                     /locus_tag="ACG808_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFS5047142.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            1016..2059
                     /locus_tag="ACG808_001513"
     CDS             1016..2059
                     /locus_tag="ACG808_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5047143.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            2065..3135
                     /locus_tag="ACG808_001514"
     CDS             2065..3135
                     /locus_tag="ACG808_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5047144.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            3125..4078
                     /locus_tag="ACG808_001515"
     CDS             3125..4078
                     /locus_tag="ACG808_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5047145.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            4090..5100
                     /locus_tag="ACG808_001516"
     CDS             4090..5100
                     /locus_tag="ACG808_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFS5047146.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(5385..6206)
                     /locus_tag="ACG808_001517"
     CDS             complement(5385..6206)
                     /locus_tag="ACG808_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFS5047147.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(6303..6581)
                     /locus_tag="ACG808_001518"
     CDS             complement(6303..6581)
                     /locus_tag="ACG808_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047148.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            6849..8858
                     /gene="metG"
                     /locus_tag="ACG808_001519"
     CDS             6849..8858
                     /gene="metG"
                     /locus_tag="ACG808_001519"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFS5047149.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(9337..9924)
                     /locus_tag="ACG808_001520"
     CDS             complement(9337..9924)
                     /locus_tag="ACG808_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047150.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            10099..11421
                     /locus_tag="ACG808_001521"
     CDS             10099..11421
                     /locus_tag="ACG808_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS5047151.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            11576..12352
                     /locus_tag="ACG808_001522"
     CDS             11576..12352
                     /locus_tag="ACG808_001522"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFS5047152.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            12345..12914
                     /gene="rnmV"
                     /locus_tag="ACG808_001523"
     CDS             12345..12914
                     /gene="rnmV"
                     /locus_tag="ACG808_001523"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFS5047153.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            12907..13791
                     /gene="rsmA"
                     /locus_tag="ACG808_001524"
     CDS             12907..13791
                     /gene="rsmA"
                     /locus_tag="ACG808_001524"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFS5047154.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            14242..14871
                     /locus_tag="ACG808_001525"
     CDS             14242..14871
                     /locus_tag="ACG808_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047155.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(14995..16104)
                     /locus_tag="ACG808_001526"
     CDS             complement(14995..16104)
                     /locus_tag="ACG808_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5047156.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            complement(16448..17767)
                     /locus_tag="ACG808_001527"
     CDS             complement(16448..17767)
                     /locus_tag="ACG808_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HFS5047157.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            17997..18419
                     /gene="mgsA"
                     /locus_tag="ACG808_001528"
     CDS             17997..18419
                     /gene="mgsA"
                     /locus_tag="ACG808_001528"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFS5047158.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      18606..18704
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            18742..19278
                     /locus_tag="ACG808_001529"
     CDS             18742..19278
                     /locus_tag="ACG808_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFS5047159.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
BASE COUNT         6171 a         3478 c         3911 g         5722 t
ORIGIN      
        1 ctttattttt ggttcattat ctaataatgt agcgaaacat ctgtcagaag tcaaaagcta
       61 gggggaaaca ttagtgaata gttatatcaa atatgtatta aaacgtgtct ttttcatgat
      121 catcacgctt tggttaattg caacaatcac ctttttcttg atgcaattgc tgccgggcac
      181 gccatatacg aatcaggaac gtttgagtcc tgaaacaatc gagatgctaa ataaacaagt
      241 cggtttggat aaaccagtta tcgtacaata tgggatttat ctttctaatt tgcttcaagg
      301 agattttgga atttccttcc aatttaaaaa ccagccagtc gcacatttgt tagctggaag
      361 aatcggacca tccttgcagt tagggttaca ggcaattatc tttggaacag tcctagggac
      421 gattttagga acgatctctg ccatgaagca aaatacatgg gcagatacat cttctacact
      481 cgtagcgata ttaggtcgtt ccattccaaa ctttgtattt gctgttttat tacaatatat
      541 ctttgcaatc aaattacaag ttttgccaat cgcaaaatgg gatggctttg tgtacacaat
      601 cttaccaaca attgcgttgg ccatgtcgcc gcttgctgac tctgctcggt ttatccgtac
      661 ggaaatggtg gaagtactgc atagtgatta tgtggaatta gcaaaagcaa aaggattgag
      721 ccgttgggaa atcgccttta aacatggtct tcgaaatagt ttgattccat tgatgacttt
      781 attaggaccg ttagctgtag cattgatgac cggatcactg gtggtagaaa atatctttgc
      841 tattcctggt atcggggaac aattcgttaa gtcaatcaca acaaatgatt atcctacgat
      901 catggctgta acgatccttt actcatttat gctgattttt gttattttga tcgtggatct
      961 attgtacggt ctagttgatc caagaattcg tgtttcagaa gggagccgag gataaatgga
     1021 aatgatacca aagcgttatg aaacagttgc agatatccct gcggatgaat ttcttccatt
     1081 acaaaaaaat accgaagaag aacgtgaaca gattactgca ccttctttga attttatcca
     1141 agattcatgg cgccgtttaa agaaaaacaa agctgccgtt atttcaatgg ttttactatt
     1201 ggtcatcatt ttgatctctg tgatcacaat ctttgtttct cctcatgatc caactgccca
     1261 aaacgtggct tatatcaatt tgcctcctcg tattccggga atcaatatcg atggattgaa
     1321 tggaaaagca atggttggtg gtgaacttgt tgataaatat gctcaagcaa acgtcccttc
     1381 aaacgtaaat ttctttttag gaacagacgg tttgggacgt gatgtattaa gccgcttatt
     1441 tatgggaatg agaatctcgt tgctgatcgc atttattgct gcattactag atgtaacgat
     1501 tggtgtagct tacgggttgg tttctggttt gttaggcgga cgtgtcgata acgtgatgca
     1561 acgttttttg gaaatcttat caggaattcc aaacctcgtt gtcatgattt tgatgttggt
     1621 cgtttttgaa ccaggaattt tttcaatcgt ggctgctatg gccatcacaa actggatacc
     1681 aatggcacgt atcgttcgtg cacaaacaat gaaattaaaa gatcaagaat atgttttagc
     1741 tggattgaca ttaggtgaat caagaatgaa aattgcattc aaacatattt tgccgaatat
     1801 ctctagtgtc attattattc aaatgatgtt tagtattcca tctgctattt tcttcgaagc
     1861 ctttttaagc tttatcggtt taggtcttac gcctccgtca gcttcattag gaacgatgtt
     1921 gagcgaaggg tacaagacat tcctgtatct gccttatcta ttatggattc cggcagtaac
     1981 tttatcggtt atcatgattg gctttaactt acttgctgat ggattgcgtg atgcctttga
     2041 tcctaaaatg aaagagtgaa gacgatgacg aaaattttag aagtgaaaga cttagaaatc
     2101 tcttttgata cgtatgctgg aaaggtacga gcgattcgcg gtgtcaattt tcatttgaat
     2161 aaaggagaaa cattggcgat cgttggggaa tctggaagtg gaaaatcagt gacgaccaga
     2221 agcatcatgc gcttgctgtc aagcaacgcc aatatagatg ctggtcaaat cctctttaaa
     2281 ggtaacgata tcgttggaaa atcagaaaga gagatgcaaa aaatccgtgg taaagaaata
     2341 gcgatgattt tccaagatcc aatgacctct ttagatccga cgatgcccat tgggaagcaa
     2401 gtcgctgagt cccttagaaa gcataataaa gtttcaaaaa aagagggaca aaaagctgca
     2461 ttagaattgc ttaaattagt tggaataccc gaagctgaga aacgtatcaa cagttatccg
     2521 catcagttct caggaggtca gcgtcaacgg atcgtgatcg cgattgcact ggtatgttat
     2581 ccggaaatcc tgattgctga tgaacctaca acagcgcttg atgtgactat tcaagcacag
     2641 attttggagc tgctgaaaga aattcagaca aaaatcgata catcaattat ctttatcact
     2701 catgaccttg gtgtagtagc gaatgtcgct gatcgtgtcg ctgtcatgta tggaggaaga
     2761 atcgttgaag ttggaacatc ggaagaaatt ttctacaacc cgcaacatcc ctatacttgg
     2821 ggactgcttg gttcaatgcc gacattagat agtgcgaacg atcgattgta tgctattcct
     2881 ggttctccac cggatttatt gaacccgcca aaaggagacg cattctatcc aagaaatgaa
     2941 ttcgcgatga aaatcgatgc agaacaagaa ccaccatttt ttgaactatc aaaaacacac
     3001 aaggcagcaa cttggctact tgctccgcag gcacccaaag tgacaccgcc agaagaaatc
     3061 caacgtcgct gggctatctt taaagagaaa caaaaacata cttacggtca aggaggaatt
     3121 gctgatgcca aataaactac ttgaagttaa aggattgaaa cagtatttca atgtaggaaa
     3181 aaagaatgaa gtacatgctg taaatgacat cagctttcac atttatgaag gagaaacatt
     3241 cggtttagtt ggagaatctg gaagcggaaa atccactact ggccgtacga tcattcgctt
     3301 gaatgaacct actgatggtg agatattatt cgacggtcaa gatataacga agatcaaaga
     3361 taaaaaaggt ttgacgaaat tccggcatga tgttcagatg atctttcaag atccatacgc
     3421 ttcgttgaat cctcgtatga aagtcagaga tatcattgcc gaaggaattg atgttaatgg
     3481 actagctaaa tcatctgaag agcgtgcaaa aaaagtcgat gatctattga gaactgttgg
     3541 attgaatcca agccacggta ctcgttatcc tcatgaattt tcaggtgggc aacgccagcg
     3601 tattggtatt gcacgagcat tggcagtaaa accgcgtttt atcatttgtg atgaaccaat
     3661 ttctgcacta gatgtgtcga tccaagccca agtggtcaat ttgttgcaag atctccaaaa
     3721 agaacatcaa ttgacctatc tatttattgc acatgatttg tcgatggtta aacatattag
     3781 tgaccgaatc ggtgtaatgc ataacggctt gttattagag atgggaacaa gtgatgaaat
     3841 ctataatcac ggtgttcatc cttatacaga aagtttgtta tctgctattc ctctaccaga
     3901 tccagaccat gagagaaaac gcagaagaat caaatatcaa cctgaaccag atgacggaca
     3961 agtacgtcaa cttcgtgaaa tcgcaccaga acattttgtc tatgcaactg agcaggaagt
     4021 cccatattac gcaaaaaaac taaaacgaca aaaggaatct cttctggttg ctaactagga
     4081 gggacataga tgctaaaagc ttttaaattc cgtatgtatc cgacagaaga acaaaaacag
     4141 caattgattc gtacttttgg atgtgctcga tttacctata accatttgct gaaaaagcgg
     4201 cagaaaagtt ggcaacaaac aggagtagcc aatttttcgc tcacacccgc tacattaaaa
     4261 aaagaatacc cattcttaaa agaagtagac agtcttgctt tagcaaatgc acagcttaat
     4321 ttggatcggg cttttcgcaa ttatttcaag ggacgtgcca gcttcccaaa gcttaaaacg
     4381 aaaaaaagta tgtggcaatc ttatacgacc aacaaccaaa cccgtacgat ctatctggaa
     4441 aatggctact taaagttacc aaaacaaaaa gaactaatca agataaatca gcatcggcca
     4501 gttgaaggat cgattcgctc cgctacgatt tctgctcgat ataatgaaga attttatgtc
     4561 gcactacttt gtgatgtttc gccagtaaaa aaagaatctt tagctaaatg gatcgggatc
     4621 gcctatcatc cgaagacatt gatccaaaca tctcgacctc tagaagtaac tttaccaaaa
     4681 tttcaccaga cagaagaaaa gctgcaacat gcacagcgga aattgaatgt gaaagtccgc
     4741 tccgctcacc atagaaagat caggttagat caggcaagca attatcagaa acaaaagaga
     4801 aaagtaatgg atctttatct gaaacaaaag aaccagcgag aagattatct tgaacaatta
     4861 tctgggaaat tggtcaaaca atatgactac ttatttgtag aatcttttcc aaaagaagag
     4921 gcacatgctg atttttctat tcacgactgg cataaattaa taacaaaatt gcgttataag
     4981 tcccaatggt ataataaaaa atttcttttg atcaataccg acggagcaga agaaagcaat
     5041 tccgtaagaa agagccaagt agtagaaaaa atgggcagac attcggttat caagggataa
     5101 aaaatgaatg aaactgatag gagcgttagg gttagccttg ttaataaaaa gtcttaatga
     5161 cttttgtttc taggaatttt tcttttgaag aaaagctcac taattatgac aaaccaataa
     5221 aacaatagca gaaaaagttc gtacgagctt tttctgctat tgttaatttt ttattcagaa
     5281 agtttacgaa tacatcaaaa ttctgaaaca aaaaacgtaa ctcacaaaga gttgtttttg
     5341 cgttttttct tgaatgatta ggtctgaatc ttttgctcac aaccttattc atggtgcccc
     5401 ggcttgagct gatagatttt tgaaggacgg ccgattccta caggacgttc accgatctca
     5461 tcgaaatatt ggagcatagc tttcttaaaa ttggaatgat cgatcgtttt ataatcgatc
     5521 cctaagaatt tagcaaatac ttttcgagct tccgtaatcg tgaaatcttt acctaaaact
     5581 tgtagaactt gcggctcgtg ttccatttta ttgactacac ggttgaatgc tttcaagatg
     5641 atttgactat ggtcaaaagc caaagtatct ttgccagaag actctccagt gactaaatca
     5701 agcgagatag ccacttctcc gctagataaa tggatcttgt tactatgacg ttctagtgtg
     5761 aaccagcgta cttccttggc atcatctccg gcgattagcg gttcttctcc aataaaggct
     5821 aagtagctga ctgtgacgac ccagcctcgc ggatctcgat taggcgtact gaatgtatgc
     5881 agttgctcaa tattttcttt tgaaataacc acaccagttt cttcctttgt ttctcttaag
     5941 acgctttctc cagttgactc atccttttgg acaaagccac caggcaatgc ccatgaattt
     6001 cgataaggat gcccttttct ttggatcaac aaaactttca gctgatcttc ttctttgtta
     6061 tagcacatca gtacgatatc gacagtcaaa gagggcttgt catattctgg acgttcctgt
     6121 ttttgatacc agttaagaaa ttcttcttca cttgctttat gttcataata taatttttct
     6181 tcttctttag aagcaaaacg gttcatctga ccacctccat tgacagtaaa agtacttttt
     6241 accttattgt agcagactct gaaaaaaaac caaggattta cttgaaaaat ctaggttatc
     6301 atttaaatat gaaaaataaa tggaagtatg agattgaata aaaagaaaag cagataaaac
     6361 ggcaatactt tataccgctc ttcacgaatg gcaaagaagc cacaaataac gatatatacg
     6421 tcaatgataa aacgcaaaat ccaagctgct gtcataaaac tgttcaaatg cccatggagg
     6481 ttaaaataac catacatacg aatataaaaa tagctgaata ttaaatgtgc taatgaaaga
     6541 aaagcaatac taagtgtaaa aaaaagatgt ttcttctgca tgattgttcc agtcccttac
     6601 taagatagat agtctttatt ataccacgtg tgaggggaga aataaaaagc cgttttttag
     6661 tagatatgaa acaaaacaaa aacccgcatt tattcaacct attcatcctt ttaaaggata
     6721 gattgaacgt agatagaagt cgtttatcca gtctttttca ttttgaaaaa aagctaacag
     6781 catgcttttt ttatggtata ctagcaagga aacgaattcg catcatgcga taaaggagga
     6841 agagccccgt ggcagaaaag gaaacttttt atatcactac gccaatctat tatccaagcg
     6901 gcaaacttca tatcggcaac tcgtatacta cgattgcatg tgacgcaatt gctcgttata
     6961 aacggttgat gggctttgat gtgttttatc tcactggagt ggatgaacat ggtcaaaaaa
     7021 tcgaaacaaa agcagaagaa ctaggtgtaa aaccgcaaga atatgtagat aaaatggcgg
     7081 ctgatgtcaa gaaattatgg aaaacgcttg atatcagtta tgataaattc attcgcacga
     7141 cagatgatta ccataaagca gctgttcaaa aaatatttga tcgtttatta gaacagggag
     7201 atatttactt aggtgaatat gaaggctggt actctgtttc tgacgaagaa ttctttacag
     7261 aaacacaact tgcagaagtg tacaaagatg acgaaggtaa cgtgatcggt ggtaaagcgc
     7321 caagtggaca cgaagtagaa ttggtaaaag aagaatcata cttcttccgc atgagcaaat
     7381 acgcagaccg tctattggaa tactatgaag aacatcccga attcatccag ccagaatctc
     7441 gtaaaaacga aatgatcaac aactttatca aaccaggatt agaggatctt gcagtttcac
     7501 gaacaacttt tacttgggga gtaccagtca agagtgatcc gaaacatgtg gtgtatgtat
     7561 ggatcgatgc attatccaat tatattactg cactaggata tggctccgaa gatgaaagtc
     7621 tattcgaaaa atactggcct gcagatgttc agatggtcgg taaagaaatc gttcgctttc
     7681 atacgatcta ttggccaatc atgctgatgg cccttgattt gcctctacct aaaaaagtat
     7741 ttgggcatgg ctggctattg atgaaagacg gaaaaatgtc taaatcaaaa ggaaatgtcg
     7801 tttatcctga aatgcttgtg gaacgttatg gtttagatgc actccgttat tatttgcttc
     7861 gtgctgttcc gtttgggtca gatggtgtct ttacgccaga agattttgtt tcccgtgtca
     7921 attatgatct ggcaaatgat ctagggaact tattgaatcg aacgattgct atgatcaaca
     7981 aatattgtga tggactcgtt cctgactatg cctcacttgt cactccattt gatagtgaac
     8041 tgtctacgac ggctgctaac gttgtcggac gttaccatga tgcaatggaa aaaatggaat
     8101 tcaatacagc attggctgaa atctgggtgc tgatttctcg tgcaaacaaa tacattgatg
     8161 aaacagaacc atgggtatta gcaaaagacg aagaaaagaa agctgaattg aatagtgtca
     8221 tgatccattt agctgaatct ttgcgtatcg cagcgatatt acttcagcca atcatgacag
     8281 aaacgccaga gaagatcttc aatcaactag gattggactc tgaaacaatg aatcttgaag
     8341 gattacactt tggcgaattt ccttcaggaa caaaagtagt tgcaaaaggt acaccaatct
     8401 tcccacgttt agatatggaa gaagaagttg cttttattca agaaaaaatg tctgaaggta
     8461 ctcaaacaaa cgaagataca gtcaaatggg atccagaaga aacagaactt gtttcaacga
     8521 aagaaaaaca aatcaaattt gatgtatttg aaaaagtaga actaaaagta gcagaagtca
     8581 tcaactgtca aaaagtagaa ggggcagata agttgctgca attccgacta gatgctggag
     8641 acagccaaga ccgtcaaatc ttgtcaggaa tcgctgaatt ttatcctgat ccaagcgaat
     8701 tgatcgggaa aaaagtcgtc attgtagcaa atctgaaacc acgaaagatg cgtggacaaa
     8761 tcagtcaagg aatgatcctt tctgcagaag caccagatgg ctctttacaa gtaatcgaag
     8821 caccaaaatc catgccaaat ggctcagaaa tcgcatagaa attcaaaggg ggtatgacac
     8881 tagttgtgtc ataccccctt tttccgaaat ttgagaagca attttcgtaa attccttctt
     8941 attacttgaa gccaatcata taatacagaa acagagaact atccaaaata aatcctagaa
     9001 aaagaagtac gagcttcgct gatttttgtt aatttagaat aatactaata ggaatcgtaa
     9061 aaacagaaaa aaagcataat gataaagggt cgggcagatt ttaattgatt catctggtac
     9121 tcttttttat tgtccttaca ttcataaagt ttgaatgcaa tgactttatt acatctatat
     9181 ttatatctaa cagtagcgtt gatgtgttaa ttataagttc tatcacttta tgttcaatta
     9241 gtttgtgctc ttatatgtgt gattgaaaat atagataaaa aaacaatcat cggctttgac
     9301 ttcgatgatt gttttcttta ttttgtaaaa atcctatcac ttgattttat ggtaaccttt
     9361 tatttttgtc ttcaaacggc ttggtaagag agcagtaagc agtgcaaatg ggatcgaaaa
     9421 gacataccag aaaatcgtac gtccgatacc ataatgttga tagagctttg tcaacatcac
     9481 gtgtaacaag atcaacccta aaagcaagga gccagctata aaccaatgat tatcccaatg
     9541 catgacttca ctaagtgcca tcactccaaa aggatagaaa taaaaataga tcccgactaa
     9601 ggtcatccgc cagttctttg taaatgttcc gatattcaat aaaaagaaaa tcgctgaaag
     9661 caaaataccg ataggcggaa aaagcccaac caaaaaggaa tacaacatcc cccacaagat
     9721 catcacagga cctttgatca atgcaacgat tgcgatgaac aaagtcagca atattagttt
     9781 ttgtgataac gttgcaacag tcattaatcc aaataaaatt aatgtgaacc aatgccaatg
     9841 ggactgctgt tgttctttac gggaaaaatc gacccggttg gataaataat ctgctaattt
     9901 cggataagaa cgcttaaaat ccataaactg ttcctccttc gtggaaaagg taggtttagt
     9961 atacgaaaaa taaaaaagaa atacatccgt cttaagaggg agtttttcac tgaaagtgga
    10021 gaaaaaagaa tggtaaatta aaatattctc agttatactg aaaattatac caaatttcaa
    10081 agaagaaaga ggatgggaat gaaaggcaaa ctaaaacgag aaatcaatct gtttggcgca
    10141 ttagctaccg ttatgggaac cgtgatcggt gcaggcgtct tcttcaaaac tgctgctgtt
    10201 actgctagta ctcagtctac aagtttgaca ttgctagctt ggcttttggg tggtttctta
    10261 acgatctgtg ctggattgac agtggctgaa ttagctacag caatcccgga aactggtgga
    10321 gctgtaaaat atattgaagc tgcgtatgga aaattgccca gctttttatt aggatgggcg
    10381 caaagcttga tttactttcc tgctaatatt gctgcattat cgattatctt tgcgacacaa
    10441 ttgacgaact tattacaatt atctaccgat tatcttttgt tgattgcagt gatcaccgct
    10501 gtttctgtga cagggttgaa tttgttaggg acaaaagtcg gtgcttctgt acaatcagtg
    10561 acgttgattg ttaaattaat accaattgct gtgattgtca tatggggatt attgacccca
    10621 ggacaaggaa ctgtccagtt atttccaatc gaagctggaa aagatgttac ttttgtcgaa
    10681 ggattgagca gtgcgttgtt agcaacgttg tttgcttatg acggctggtt aggtgtcggt
    10741 gcaatggcag gggaaatgaa acggccggaa aaagatttgc ctaaagcaat tattcttggt
    10801 ttgagttttg taacagtcgt ttatctattg atcaattttg tttttttgaa aactttgccg
    10861 attgatcatc ttgccggtaa tttgaatgca gcttctgaag catcagacgt catttttgga
    10921 ggaatcggtg gaaagctggt cactattggg atcttgatct cggtatacgg tgcattgaac
    10981 ggttatacac taactgggat ccgcgtgcct tatgcgatgg ctttagaaga cgagctacct
    11041 ttcagtaaac agctgacaaa gctttcaaag aagtttacgg ttccttatgt tcctgcggtt
    11101 ttccagttag cagtagcttg tattatgatg agccttggtt ctttcgactt tttaacagac
    11161 atgttgattt ttgtgatgtg gctattcagt ttactgattt ttattggtgt ctttatttta
    11221 agaaaaaaag cgccggaatt gccacgtcca tataaagtac cactttatcc aatcgttccg
    11281 attattgcga ttctcggtgc tattttcatt ttaggtatga cgatgatgac gcaaacgaaa
    11341 ttagcattga tcggtatagg tgttaccttg ataggtatcc cagtctatta tcaaaaaaag
    11401 aaaaaacgct cagaagagta aaatatttca acaacagaca gaaaaagtac gaactaggtc
    11461 ctctagatct tcgtgctttt tctatttcaa taaaacaagt tcttctattt tgatttttgc
    11521 tacttctgca agtagtgctt atgtgctaaa gtaagaatga aaaggagaga taaacatgat
    11581 atttgattct catacccatt taaatgctga acagttcaat gaagatattc cagaaacgat
    11641 tcagcgagca caagaaatgg gggtaacgaa aatggcggtc gtgggcttcg atacgccaac
    11701 gattgaaaag tctttgcaat taagccatga ctatccaaat atatacagta ttatcggctg
    11761 gcatccgaca gaggctggca gttatacgaa agaaatcgaa aataaattac aagaacaatt
    11821 gactttacca aaggttgtag cattagggga aattggcttg gattatcatt ggatggaaga
    11881 cccgaaagaa gtccaagata aagtatttcg cagacagatt gctatcgcaa aagaaatgaa
    11941 cctaccaatc agtattcata ctagagaagc acttgaagac acgtatcaaa tcctaaaaga
    12001 agaagatgtt cgagatatcg gtggaatcat gcatagtttc agtggtgatt atgaatgggc
    12061 aaaacgattt cttgacttag ggatgcatat ttcatttagc ggtgtcgtca ctttcaaaaa
    12121 agcattagac gtccaagagg ctgcagcaaa tgtaccaatc gaacgattac ttgtggaaac
    12181 agatgcccct tatctggcac cagttccttt tagaggaaag cgaaatgaac caggctatac
    12241 tcgttataca gtagaaaaga ttgctgaact tcggaatctt ccgacagaag aagtggctgc
    12301 ccaaacatgg acaaatgcgc accgattatt tggtctaagc gaacatggat aagatgaaga
    12361 tccaagagat tatagtcgtt gaaggaaaag acgatacaag aaggctgcgg gaagtagttg
    12421 atgcagatac gatagaaaca atagggtctg caatcaatga agagatcctg atgcaaattg
    12481 aacatgccca agagactcgt ggagtgatcg tttttaccga tcctgatttt tcaggagaaa
    12541 aaataagaaa aatcattatg gaagctgttc ctcaggcaaa acatgctttt ttgccgcgca
    12601 cacaagctaa accaaagaaa aagggaagcc ttggtgtcga gcatgcaagt gatgctgcta
    12661 ttttagaagc attgaaaaag gtcgtgacgc cagacatttc ccaaacggct gtttctgaaa
    12721 tcactagaca agacctttta gactatggtt taatagctgg ggcacaagca aaagaaaaac
    12781 gagaaaaact aggtgacgag ttgcgcatcg gttacacgaa cggcaaacaa ttggaaaaac
    12841 gtttgaagat gtttcgtatt acgccgaaag aactcgccca agcaatgaaa atagtggagg
    12901 aacaacatga gtgaatatcg tgaaattgcg acaccctcta gaacaaaaga gattttattg
    12961 aaacatggtt tttcatttaa aaaaagttta ggacaaaatt ttctgactga accaaatatt
    13021 ttaaggaaaa tcgtagaaac tgcgcaaata gatgaacata cgaacgtgat cgaagttgga
    13081 ccaggaatcg gtgcattgac ggaacaatta gccaagcatg caaaacaagt cgtagcattt
    13141 gagatcgatg atcgtttgat ccctgtattg gctgatacga tgcaacccta tgacaatgtg
    13201 actattgtac atcaagatat tttaaagacc gatttgtcaa cagctgttag agaaacattt
    13261 catgaggagc ttccgttaaa agtcgtagcg aatttacctt actatattac gacaccgatc
    13321 atgatgcatt ttcttgaatc agatttggtt gtggatgaac tagtcgtcat gatgcaaaaa
    13381 gaagtagctg atcgtatctc tgctgaacca ggaacgaaag cttatggttc attatcgatt
    13441 gctgttcaat attatatgga agccagttta gcattcatcg taccaaaaac agtatttgtc
    13501 cctcagccaa atgtagattc agcgattttg aaactgacaa gaagagatat accggcagtc
    13561 gaagtgacag acgaaaaagc atttttccga ttgacaaaag cggcatttca gcaaagaaga
    13621 aaaacgcttt ggaataactt gcagcattcg tatggcaaag atgaccagac aaaagcatgg
    13681 ttagcaaaaa gcttggaaac agctggaatc gatccaaaac gtcgtgggga gactttgtca
    13741 ttacaagaat tcgccgcatt aagcaatgcg atgtcagaaa atatgcagta aaaaaagagg
    13801 ttgtgacaga agttgtgaaa aagctgtcct gcatcaagta ataagaacag ccaaacaaaa
    13861 atagttcctc atgtttttcg tttgtttggg cttattaccg cagatgcaag cttttgaaca
    13921 ccgtttaaga aagaggttgt gggcctgccg tttaactcta agtcacaagg aacaatttca
    13981 aaaaacagct tctcatgttt ttgagttagt tggtcttatt acttgatgca agcttttgac
    14041 cactattatg ccgaacaagg agatgtgaca caaattttgt cacatctcct atttttgttt
    14101 cttgatcaaa aaattcttat acttgatact cacactcatt ttgtatggat ttagaaaaga
    14161 atatgtgtct tgacagttat tagtaagtga gttatactaa gtcgacattt atgtgtcaag
    14221 acatattagg agagagaaaa aatgagaaaa aattcagttc gaaaactaac gatttctgct
    14281 ttattgattg ggatgggggt cattatccca atggtcatgc ctaaaatcat gattccacca
    14341 gcttccttta ctttggcgtc tcatgtgcca ttatttattg cgatgttttt tacaccagga
    14401 gttgcagtgg ctgttgcgct aggtacgaca tttggtttct ttttaacaac gccggttatc
    14461 atcgcattgc gagcattgtc acatcttgtg tttgcgttga ttggggcttg gtacttgcaa
    14521 aagcatccga gtatcgtttt aaaaaatgga cagtttactt ttgctaattg gcgtttccaa
    14581 gggttcaatt ttgtgatcgg tgtgattcat tctttagcag aaatgctagt agtcagcatc
    14641 ttttacttta ttggtaatat gcctgaaaca tattattctc aaggatattt ttatacagta
    14701 ttcatcttga tggggattgg tgggcttatc cacagtttag ttgattataa tattgcttat
    14761 tttatagcag gaacactcag caaatatttt gatatccccg tatttacggc agcaaaaaaa
    14821 gaaaatcgga aagagtcgat tcaagggtac gcgaagaaaa cgacatatta gacatgatct
    14881 ctcaaaaaaa aaactctcat ctagtaatgg ataataaatc ctctaagatg agagtttttt
    14941 gtcctaaaaa gagctgcacc aacatcttct ggcacagctc taacaggggg caatttatgg
    15001 aagtttgatg acttgttctg tttctttatc aaagaagtgg cctttgctga tgttgaaggc
    15061 aaggtcgact atttcgcctg gtttatggaa atctcttgca tctacttttg agatgaactc
    15121 agtagaacca gtttttgtgt aaagcatcgt ttcagctcca agtaattcgg aaacgacaac
    15181 ttctgatttc actacggctt caggtgaagc ttcaagggct acttgttcac tgtggatgtc
    15241 ttctggacga ataccgaaga taacttcttt tccttcataa cctttttcaa ctaatacttt
    15301 gttttttcct tcaggaatac gaagcttaag cccatgtcca tctgtgataa caccattttt
    15361 caatgtcaca ttaaagaagt tcatggcagg agatccaata aagccggcaa cgaacatatt
    15421 gacgggtgta tcataaactt ctttcggtgt accgatttgt tggataaatc cgtctttcat
    15481 gataacgatt cggtcagcca tagtcattgc ttctgtctga tcgtgagtaa catagatggt
    15541 cgttgtttca agacgctggt gaagtttggc aatctcagca cgcatggcta cacgtaattt
    15601 agcatctaaa tttgataatg gctcatccat taagaagact tttgcatccc gaacgattgc
    15661 ccgtcccaat gccacacgtt ggcgttgtcc accagataaa gcagctggtt ttcgtttcaa
    15721 gtattctgtc agacctaaga tctctgctgc attgtccaca cgttttttga tgtctgcttt
    15781 atcatattta cgtaatttca agccaaaagc catattatca aaaacagtca tatgaggata
    15841 caatgcgtag ttttggaaaa ccatggcaat atcacggtct ttaggtgcca cgtcattcat
    15901 gacttttccg ccgatattta gttctccttc agagatatct tccagtcccg cgatcattcg
    15961 caaagtagtt gactttccac aaccggaagg accaacaaaa acgataaatt cacggtcttt
    16021 gattgataga ttaaaatcag ttacagaata attttctgca ttgtcatatt ttttatatac
    16081 gtttttcaga gccatttcta ccataatacg ttccacactt tcttttttag ttttattaac
    16141 tatttaaagt ataaatgaaa gcgttaacta acttcaatgt catgttgaac aagaattttt
    16201 tctttttttt gtcaatgtgc tagttttaag ctttaataaa tatccctgaa taattcatag
    16261 ctttaattct ttggcacatt ttattgaaat atgtatcact aatatcccta tcaactgatt
    16321 ttgactaaaa agttacttca aacattcact gttcaaaaat attgaggaat aaattttggg
    16381 tgtaaggcat tttgagcata cttctccctg gataacttcc cagtaaaaaa atcgttagat
    16441 tgttggatta gatccattga ctggctcgcc tcaggcgagc aaagaccctg tagaattcat
    16501 tctgatacac atgataacta gataatttga gatagactcg tctacccgtt tggactaatt
    16561 tcccagcaac tttcagaaac ttcaatcgaa tagaatgaat ggtcattccc ttttggactt
    16621 cttcaaagcc aatcgttctt aagaagttga ctaagttgta agctatcaag ctgagcatca
    16681 ttcggacatg attctccaaa aagcgaggac tgtctgtttt gtcaaagtaa aagccagctt
    16741 tcgcttcttt aatgaagttc tccattgtgc cacgtttggc atagatagaa aagatggtat
    16801 caggagaaac attttctgat agattcgtca cgataaattc atgtcgaaag agtagttcgc
    16861 ccgcttcacg tgttgagcgt atacacacgc gacgactttg tgaccaggat tgtgcttgat
    16921 aagtggtcga gaagtactga acttctcgtt cttcccactt ttgattatcg ccataaagta
    16981 ctgatttctc agctatttga cctagtctac gattattctt cagtcgaata acataatgac
    17041 tcttttttga ttcacacgaa tcatacacac caggtgtagc gaacccgctg tctccacgaa
    17101 ccaagatgtc agtgtttggt agagagtgat tatagtgctc taataaaggt gtgagaaact
    17161 cctttacgcc ttttgatgta tattgatttc ctgaacgtag ttcagctttt aagaagtcac
    17221 cagtcaatcc gtcaaaagct accaatggat ggtaaccata ggtttggtag tgtgtattat
    17281 aattcgtttg ttcttggtga ccaaatgtat ctgaatgggt cgaatctaaa tcaatgatta
    17341 attccgtatc attacggatg aggcgtgctt tatcgataag ttcttggttc aaagcttgaa
    17401 gttcatggat attctcctca gatagtcgat ctaaaaatcg ggaaagtgaa gattgagaag
    17461 cgagttcttt cctatctaaa acagctttaa acacaggatc ttgtctcagg agattagctg
    17521 cagaatcagc tgagtaacca gcaattaatt gcataatgaa ctgctcaagt atggataagt
    17581 tgtcatgagt aaaatacgct cgttcgtctt caatgtgaat gtgttgctta gccagttcag
    17641 aaaaaccaaa gatgttcatc agctctttca ctaggacgag acccgaatca ctcgataatt
    17701 gaccacctgt atgagaaata gtgatatttg aattgaattt tacttggttt ttgtgtaagc
    17761 tagtcatgaa aagaactcct ttcttttggt tggtttagtc actttaacca tagcagaaag
    17821 gggttctttt ttcatcactt aacgggtgaa gatgaaaaag taattgtaag ctgccgtgag
    17881 gcagtttcac atggttttaa ttaaaagtat gaattattca ggaatatcca taagaaataa
    17941 aaactgatac actggagtaa ataagtatgt taacaatatc aaaaggagtg tctcttatga
    18001 aaatcgcttt gatcgctcat gaccgaaaaa aacctatgat ggtgaaatta gcaacagcgt
    18061 ataaaacaat attggaaaaa catgaacttt ttgctaccgg gacgactggg cagaggatca
    18121 cggaagcaac cggcttgccg atccatcggt ttaaatccgg accgttaggc ggggatcagc
    18181 aaattggagc catgatttcg gaggatcagt tagatttggt gatttttctt cgagacccgc
    18241 tggctgcaca gccccatgaa ccagatgtaa cagcacttat ccgtttatct gatgtctatg
    18301 agattccgtt agctacgaat atcggaacag ctgaggtgct actaagagga ctggaagccg
    18361 gcttcctaga ttttcgaaga atcgtccatg aaatagacaa gcatccactt tcattttgat
    18421 ggataagcgt atggaaaaat aacgtttaat gttcgcctat atgtagctat aaaagctaaa
    18481 caaactgata atatgcgata taatacgaac gtattggata atataaacaa taatattagt
    18541 ttttcgtatt tgcagtaaat gtgaaaatat gctatagttt ctgtgggaaa agatcccaaa
    18601 aataatacag agtagaaaat aaagcgttaa gtgctaagag gatgggatgt tgcctcttgg
    18661 acgaaggacg taatgttcgc ggttttattt tcgcattcgc tgcatgatac gatgtagcag
    18721 ctcttaaagg agatgctaaa aatgaagaaa caccggttag acgcacgtat gattgctatt
    18781 atgggcttat tgattgcatt gatggtcact ttgtcccgac tggtagcaat cgagacccca
    18841 tttattaaaa tcagtgtcac atttattcca caagtgatca tgggcattct ttttggtcct
    18901 ttttggtcag ggataggcgc agttttagct gatttagtag gaatggccct cttctcgaaa
    18961 tctgcattct ttattggatt tacattgaat gcatttattg aaggagcaat ctacggattc
    19021 tttttctatc gaaaagagat cacttggaaa aatgccattc tggctacgct tagtgtcacg
    19081 ctgatcatca acttattttt gacaccatta tggcttgcgt tgatgtacca tgtgccattg
    19141 ttcagctggg tcgtttgggc gccccgcctg ttaaaaacag taatatggtt accgattcaa
    19201 tcaattgcca tttattacgt aggacgtagt attccttaca aaaaaatttt aagaagttta
    19261 gcgatccacg ctaaataaaa aa
//
LOCUS       DAZUMK010000047        17737 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000047 DAZUMK010000000
VERSION     DAZUMK010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.048"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            26..838
                     /locus_tag="ACG808_001530"
     CDS             26..838
                     /locus_tag="ACG808_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFS5047160.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            835..1635
                     /locus_tag="ACG808_001531"
     CDS             835..1635
                     /locus_tag="ACG808_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFS5047161.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1796..3064
                     /gene="rseP"
                     /locus_tag="ACG808_001532"
     CDS             1796..3064
                     /gene="rseP"
                     /locus_tag="ACG808_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFS5047162.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3132..4841
                     /locus_tag="ACG808_001533"
     CDS             3132..4841
                     /locus_tag="ACG808_001533"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFS5047163.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5053..9405
                     /locus_tag="ACG808_001534"
     CDS             5053..9405
                     /locus_tag="ACG808_001534"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFS5047164.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9549..10022
                     /gene="rimP"
                     /locus_tag="ACG808_001535"
     CDS             9549..10022
                     /gene="rimP"
                     /locus_tag="ACG808_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFS5047165.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10046..11221
                     /gene="nusA"
                     /locus_tag="ACG808_001536"
     CDS             10046..11221
                     /gene="nusA"
                     /locus_tag="ACG808_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFS5047166.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11243..11536
                     /locus_tag="ACG808_001537"
     CDS             11243..11536
                     /locus_tag="ACG808_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFS5047167.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11533..11844
                     /locus_tag="ACG808_001538"
     CDS             11533..11844
                     /locus_tag="ACG808_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFS5047168.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11857..14163
                     /gene="infB"
                     /locus_tag="ACG808_001539"
     CDS             11857..14163
                     /gene="infB"
                     /locus_tag="ACG808_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFS5047169.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14190..14537
                     /gene="rbfA"
                     /locus_tag="ACG808_001540"
     CDS             14190..14537
                     /gene="rbfA"
                     /locus_tag="ACG808_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFS5047170.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14645..15149)
                     /locus_tag="ACG808_001541"
                     /pseudo
     CDS             complement(14645..15149)
                     /locus_tag="ACG808_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15314..17677
                     /locus_tag="ACG808_001542"
     CDS             15314..17677
                     /locus_tag="ACG808_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HFS5047171.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
BASE COUNT         6046 a         3148 c         3907 g         4636 t
ORIGIN      
        1 tcttttaagt ataggaggaa aaagaatgtt acgttttttt ccgcaaaaaa acaaatatat
       61 tcaagaaaaa agcgaatttc aatttaatac cgatggacca attcctcagc acattgccat
      121 catcatggac ggcaatggtc gttgggcgca aaaccgtcgt ttgccacgag ttgctggcca
      181 taaagaaggc atggaaacag taaagaaagt cacaaaaaaa gcatcaaggc ttggagtgaa
      241 agtgttgacg ctttatgcct tttcgactga aaactggaag cgaccaaaag atgaagtaag
      301 ttttctcatg caactacctg tcgacttctt tgatacgttc gtaccagaat tgattaaaga
      361 aaatgtcaaa gttcatgtca tgggttatga aaacgtattg ccagaacata cgcaagatgc
      421 tgtaagacgt gcaattgagc aaacaaaaaa caatactgga atggtattga attttgcttt
      481 gaattatgga agtagagcag agatcgtgac tgctgtccaa gaaattgccg aagaagtagc
      541 aaaaggagaa atacacgcag aagaaatcga tgatgaattg attgctaagc atttaatgac
      601 aggtttcttg cctaaagagt tacaagatcc tgagctgatg atccgtacaa gtggagaaga
      661 gcgaattagt aattttctgt tatggcagat cgcatatagt gagctgtatt ttacaaaagc
      721 gctttggcct gattttgatg gtgcgcatct tgaagaagca atcgcctctt atcaaaatcg
      781 agaccgtcga ttcggcggtg taaaaaaaac agaagatgag aaaggggatc agtcatgaaa
      841 caacgtgtga tcacagcagc tgtggcgcta gtcttgttta ttccgattat atggatcgga
      901 ggcatagcgg ttgagcttac agcagcaatc ttagcggtag taggagttta cgagctgttc
      961 cggatgaaag gattgacact cttaagtttt gaaggagttt tatccgccgt tggtgcagtg
     1021 ttacttgttt tgccaaaaga aagatggttt ttctttcttc cagagaatgt aagtacattt
     1081 atgttatttt atcttatagt tatgattctt ttaggaacat cagttatttc taaaaatacg
     1141 tatacgattg atgaagcagg ttttcctgtg cttgtcagct tgtatgtggg aataggtttc
     1201 cagaattttg tcaatgcacg aacagaaagc ttggctgttt tgatatttgg tctatttatt
     1261 gtttgggcaa ctgatatcgg agcatatatg gttggtcgac gttatggtca gcataagtta
     1321 agtcctgata tttcgcccaa caaaacgatt gaaggtgcgc tcggtggtat cggtagtgct
     1381 gttgtagtcg catttcttta tttattgcta tacccagcaa aagaactatt caatcatggt
     1441 atgtttgtca tgcttctgat gacgattatt ttttcagcag tcggacaatt tggtgatttg
     1501 gtcgaatctg caatcaaacg tcattatgat gtgaaagact cgggaaatat cttaccaggc
     1561 catggtggaa tcttagaccg tttcgacagt ttgctgtttg tatttcctat catgcattta
     1621 tttggcatta tttagtaagt atacctgcgt ttctttgtga atttcataaa agacgcaggt
     1681 ttttattttt cattatattg atactattgg tgtctttggt atactatggt aaaattatag
     1741 tgttttatcg ttgttttata aaaaacacaa aaaacaagta aaggagaaaa aagctatgaa
     1801 aacgattctg acatttatca tcgtttttgg tatattagtg attgttcatg agtttggtca
     1861 tttcttcttt gcgaaacgat caggaatcct cgtccgcgaa tttgctatcg gtatgggtcc
     1921 gaaaatttat gggcatcaag caaaagacgg gacaacatat acgcttcgcc tattacctat
     1981 tggcggttat gttcgaatgg caggcaatgg ggatgatgaa acagaaatgg caccaggaat
     2041 gcctctttct cttttactaa attctgacgg catcgtagag aagataaatt taagtaagaa
     2101 aatccaattg acaaatgcta tcccgatgga attgagccgt tatgatttag aagatgagct
     2161 gactatcact ggttatgtga atggagacga aacagaagta gtaacctacc ctgttgatca
     2221 cgatgcgacg attattgaaa acgacggaac agaaatcaga attgcgccaa aagacgtaca
     2281 gttccaatcg gctaaattgt ggcagcgtat gctgaccaac tttgctggac ccatgaataa
     2341 ctttattttg gcaatcgtgt tattcatcat tctggcattt atgcaaggtg gtgttcaagt
     2401 gacgaacact aatcgcgtag gagagatcat gcctaatgga gctgcggcag aagcaggctt
     2461 aaaagaaaat gatgaggtag tcagtgtgga cggaaaagaa atccattcat ggaatgatct
     2521 gacaacagtc attacgaaaa atccaggtaa aacattagac tttaagatcg aacgagaagg
     2581 tcaggtgcaa tctgtcgacg tgacaccgaa atctgtagag tcaaatggcg aaaaagttgg
     2641 ccaacttggt ataaaagccc caatgaatac aggttttatg gataagatca ttggagggac
     2701 gcgtcaagct ttcagcggtt cattggaaat tttcaaagcc ttaggatcac tatttacagg
     2761 tttcagttta gataaattag gcggacctgt catgatgtac caattgtctt cagaagcagc
     2821 aaatcaaggt gtaacaactg ttattagttt gatggcgctt ctttcaatga atctcggaat
     2881 cgtcaatctg cttccgattc ctgccttaga tggcgggaaa ttagtcttga atatttttga
     2941 agggattcgc gggaaaccat taagtcaaga aaaagaagga atattgacat tagctggctt
     3001 tggattttta atgttgttga tggtgctagt tacatggaac gatattcaac gattcttttt
     3061 ctagaaaaag ttgacaaata gggtataata acaaacgata actattttga ggaaataaag
     3121 gagtatatag aatgagacaa tcaaaaatgt tgattccaac attacgcgaa gttccaaatg
     3181 atgcagaagt attaagccat caaatcttgc taagagctgg atatatccgc caagtatcag
     3241 cgggtattta ttcatatctg ccattagcaa atcgtgtatt ggaaaaactg aaacgcatta
     3301 tgcgggaaga atttgaaaaa atcggagcgg tagaaatgct gatgcctgcc atcttgccag
     3361 cagaactttg ggaagaatcc gggcgttacg aaacatatgg accaaacttg tatcgtttca
     3421 aagaccgcaa tgatcgaaaa atgatccttg gaccaactca cgaagaaact tttactgatt
     3481 tgatgcgtaa tgaaatcaat tcatataaga aattaccttt aaatctttat caaatccaac
     3541 ctaaatatcg tgacgaaaaa cgacctcgct tcggtttgct tcgcggaaga gagtttatca
     3601 tgaaagatgc atattctttc catgcaacgg aagaaagttt ggacgagaca tataaagatt
     3661 acgaaaaagc ctataccgaa gtattcaagc gttgtggctt agaatttcgc tcgatcatcg
     3721 gagacggcgg cgcaatgggc gggaaagact caaaggaatt catggcgatc tcagaaatcg
     3781 gggaggatac gatctgttat tcgactgaaa gtgattatgc tgcaaacttg gagatggcta
     3841 caagctacta tgtatcaaaa aaatcgcatg aaacacagct tgaattggag aaagtagcta
     3901 cacctaacgt aaaatcaatt gacgaagtag cagatttctt ttctgttgaa cctcaaaaaa
     3961 tcatcaagtc tgtattgttc attgcggatg aacaacctgt ccttgttttg gtacgtggag
     4021 accatgaagt caatgacgta aaactgaaaa actttttatc tgctgacttt ttagaagaag
     4081 caacagaaga agaagcacaa aaatatctag gtgctgagtt tggttctgtc ggaccagtcg
     4141 gtgtttcaga tgacgtaaaa gtatatgcag accgttatgt ccaagattta gcgaatgcca
     4201 ttactggagc gaacgaaaca ggattccact ataaaaatgt gaatccaaaa cgcgatttca
     4261 ctgtgctttc ttatgaagat cttcgttttg tacaagaagg agatccttca ccagacaaca
     4321 acggtgttct tgcctttact cgaggaatcg aaatcggaca tatattcaaa atcggcactc
     4381 gttacagtga atctatggga gcaactgtac tagacgaaaa tggacgagaa aaatttgtga
     4441 ttatgggctg ttatggtatc ggtgtaagtc gtttattatc agcaatcgta gaacaacatt
     4501 cagatgaaca aggaatcaat tggccaaaag gaattgcgcc atttgatctg catatcgtcc
     4561 aaatgaatct aaaagacgaa taccaaacaa gcctgacgaa tgaactagaa gaagcgatga
     4621 caaaagctgg ctatgaagta ctggttgatg acagaaatga acgcgcagga gtgaaattcg
     4681 cggattctga tttgattggc tgtccaatca gaatcactgt cggtaaaaaa gcagtggatg
     4741 gaatcgtaga agtgaaaatc aagaaaactg gcgaaatggt tgaagtacgt aaagaagaat
     4801 tggcgaatac tttaccaatc ctcttgaacc aaggcgaata ataagcgtta aaacacagaa
     4861 aataaaaata gctgaatcag ctgtttttga caatctgatg attcgctttt aaaattggaa
     4921 ctgcgatttg aatcgcagtt ccaatttttt aatgtaaaac tcagaaaaaa cgagtatact
     4981 aaaaggtact gaaaaagaag aggataagat gcgatcaatt gcattgtttc tcatcagaaa
     5041 gggcgtatat ctttgtcaga aaaacgagat ttatttgaaa aactgttgga acaaatccaa
     5101 ctagaggaag cagaaaaaaa tcatccactg cttactgctg gtgaaatgga tagtgtagtg
     5161 gtccatcgta aatcgcgttt atgggaattt acgctacatt tttccaaaat tttgcctatt
     5221 atgctatatc ggtcattgac tcagcatctg actatcgctt ttaaagatat tgcacaagtc
     5281 aaactgaata tcttagcaga tgaccagaca ttcgatgaac agctcttgca agattactgg
     5341 gcccaagctt tggaaaatca gcaatgcgat acaccactag cacagcaagt attgaaaacg
     5401 caggtacctg tgataaaaga tcgtaaagtc atcttgccaa tcgacagtac aggagctatt
     5461 tcttatttga agcaacaata cttgccatta gtagaagagt tgttcgtcag ttacggattc
     5521 ccaaaatttc gtatcgaacc agaagtggat gagcagcaag cggaacgtgt attgaaactt
     5581 tttgaagaac gcaaacaaga acaggcagaa gcctttatga agcaagcagc tgaaagtcta
     5641 gttgtccacg aacaaaagaa aaaggaaaga aaagagcaat cgccagctct tgaaggaccc
     5701 atccagctag gacgaaatat tccagccgat gaaccaacga caccaatgat cagtatcgtt
     5761 gaagaagaac gaagagtcac acttgagggg tacgtattcg acaaagaagt cagagaacta
     5821 cgttcaaaac gcaagatctt gactttgaaa atcacagatt atacttcatc atttatcgtc
     5881 aaaaaattct cgaacggcga aaaagatgaa caagtatttg atgcgatcag tgtcggtagc
     5941 tggttaaagg tacgaggaag tattcaagaa gatacgtttg tccgtgattt ggtcatgaat
     6001 gcgcaagata tcatagaagt caaacataca cctcgcaaag attatgctcc tgaaggggaa
     6061 aaacgagtag agcttcatgt tcatagcaat atgagtacga tggatgcaac aaacagcatc
     6121 tctgatcttg tggcacaagc aggaaaatgg ggacaccgag ctatcgccat tacagaccat
     6181 ggtggtgcgc aagcctttcc ggaagcacat agtgctggaa aaaaagcagg tgtgaaaatc
     6241 ttatacggcg tggaagcgaa tgtcgtagat gatggtgtcc caattgctta taatgatgca
     6301 catgaagcat tgagtgaagc cacatacgtc gtatttgacg tggaaacgac aggattgtct
     6361 gcagtatatg acacgatcat cgagctagcc gcagtcaaaa tgtataaagg aaatgtgatc
     6421 gaaagctttg atgaatttat cgatccagga catccattgt cacgaacaac ggtagatctg
     6481 actggtatca cggatgaaat ggttcgaggg tcaaagtcgg aagaagaagt attgcggatg
     6541 ttccttgaat tttcaaaaga tacaattttg gtagcccaca acgcggcttt tgatatgggg
     6601 ttcttgaata ctagctacgc aaggtatggt attcctgaag cggccaatcc agtcattgat
     6661 acgttggaat tagctagata tctgtatcct caattcaaac gtttcggatt aggtgtgtta
     6721 tctaaaaagt ttggtgttag cttggaacag catcaccgag caatctatga tgctgaagca
     6781 actggtcact tagcatggat ctttgtgaaa gaggcaatgg acaatcataa catgctttat
     6841 catgatcagc taaatgagca tatcggagaa ggagattctt acaaaagggc tcgacctttc
     6901 catgtaacga ttttagcaaa aaatcaggca ggactgaaag atttgttcaa actgatttca
     6961 atgtcgaatg tcgaatactt cgaacgcgtc ccacgaatac cacgctccca attgaaaaag
     7021 atgcgtgaaa atctattgat cggctcagct tgtgataagg gagaaatatt cgaagccatg
     7081 atgcaaaaag gggtagaaga ggcaagaaac cgagcaaaat tctatgatta tattgaagtt
     7141 atgccaaaag cggtctatgc gcctttgatc gagcaagaat tggtaaaaaa tgaacatgat
     7201 ttggaagaaa ttatccaaaa tttggtagaa atcggaaaaa gtttggataa aatcgttgtt
     7261 gcaacaggta atgtacatta tctgaacgaa gaagatgcca tttaccgaaa aattttgatc
     7321 aattctatgg gcggagcgaa tccgttgaat cgtcatagtt tgccggatgt ccatttccga
     7381 acaaccgatg agatgctaac tgcatttcac tttttagggg aagaaacagc gaaagagatc
     7441 gtagtagaaa atacgaataa aatcgcagat atctgcgaag aagtgattcc agtcaaagat
     7501 gaactttata caccgaagat tccaggttca gaagatgaaa tatcagaatt gagttacaca
     7561 aaagcaaaac aaatgtacgg agatccgtta cctgagatca ttcaaaaacg attagaaaaa
     7621 gagttgaatt ctattaatgg taatggcttc tcggttattt atctgatcgc ccaaaaacta
     7681 gtgcataaaa gtaatgaaga tggctatcta gtaggttcac gtggctctgt tggctctagc
     7741 tttgttgcga cgatgacagg aatcactgaa gttaatccat tagcccctca ttattattgt
     7801 ccagagtgcc aatattccga gttttttgaa gatggtacgt atggttctgg atttgacatg
     7861 ccagaaaaac aatgtccaaa atgtggtgca cgcttaaaca aagacggtca tgatattcca
     7921 tttgagacat ttttaggatt ccatggagac aaagtgcccg atattgattt gaacttctca
     7981 ggtgattatc aagcagaagc ccataactat acgaaagtat tgtttggcga agactatgtt
     8041 taccgtgccg gaacgattgg tacagtcgcc gacaaaacag catatggcta tgtaaaagga
     8101 tacgaaaggg acaataatct gcagtttcga agtgcagaag tcgacagatt agcaaaaggc
     8161 gctactggag tcaaacggac aaccggccag catccagggg ggatcatcgt tattcccgat
     8221 tatatggatg tctatgattt tacgccgatc caatatccag cagacgatca aaattcagaa
     8281 tggaaaacga ctcattttga tttccactcg attcacgata atgtattgaa attagatatt
     8341 ctagggcatg atgacccaac tgttattcgg atgcttcaag atttatcagg gatcgaccca
     8401 caaacgatcc ctacggatga cccggaagtg atgcggatat ttgccggtcc tgaagtactt
     8461 ggagtaagtc aggaacaaat ttattccaaa acagggacat taggtattcc agaattcggc
     8521 actcgttttg ttcgagggat gttagaagaa acgcatccta caacttttgc agaactactg
     8581 caaatttccg gactatctca tggtacagac gtatggttag gaaatgcgga agaattgatt
     8641 cgacgaggag atgcgacact ggcggaagta atcggctgtc gtgatgatat tatggtttat
     8701 ttgatccatg ctggattaga tagcgggatg gcatttaaaa tcatggaaac tgttcgtaaa
     8761 ggacaatgga acaagatccc tgatgaattg cgagaaacct atctatcggc gatgaaagaa
     8821 aataatgtgc ccgactggta tatcgattct tgttcaaaaa tcaaatatat gtttccgaaa
     8881 gcccatgctg cagcttacgt attgatggct cttcgtgtcg cttatttcaa ggtttatttc
     8941 cctattttgt attactgtgc atacttttct gtacgtgcag acgactttga tctagtctcc
     9001 atgtgcaaag gaaaagatgc tgtgaaacaa gcaatgaaag aaatcacgga caagggatta
     9061 gatgcttccg taaaagaaaa gaatcaactg acagtgctgg agttagccaa tgaaatgctg
     9121 gaacgaggat tcaaatttgg aatgattgat ttgtataaat cggatgccgt taactttgtt
     9181 attgaaggtg atacgttgat cgcaccattt agagctgtcc caagtttggg aaccaacgtg
     9241 gcgaaacaaa tcgttgaagc aagaaaagac ggtccatttt tatccaaaga agatcttgcc
     9301 acccgcggta aagtttcaaa aacattgatc gaatatatga acgacaatgg cgtattgaaa
     9361 gatctaccag atgaaaatca attatcgtta ttcgatatgc tataagcatc tgttttcctg
     9421 tctgcttgta attttaagca gagtatggta tagttcttat aggtaatgat actaactgtg
     9481 agtgagcgga tttttcgctc actcttttta atggaattac gcacttaatt tttaaggagg
     9541 cgaaactttt gagtagcgtc gttgaaacag tcacagaaat ggtgacaccg attttagatg
     9601 aacaaaattt tgaacttgtc gaagtcgaat ttgtaaaaga aggaaaaagc tggttcttac
     9661 gagtatttat tgacaaagaa ggcggtatcg atatcgaaga atgcgccttt gtcagtgaga
     9721 aactgagtga aaaacttgat gcagcagatc cagatccgat tcctcaagct tatttcctgg
     9781 aggtatcctc cccgggagca gagcggccat tgaaaaaaga aagcgactat gaaaaggcac
     9841 ttggtgatta tgtccatgtc tccttatacc aaccagtaga tggcgaaaaa caatatgaag
     9901 gatttcttca atcatttgat gctgaacagc ttacattgaa aatacgcata aaaacacgag
     9961 aaaaagaatt gacttttgac cgaaaaaata ttgcaaaagc tcgcttagct atccaatttt
    10021 aatcacggag aggaatcaga aaaaaatgag taaagaaatg ttaaatgcgt tagacgcttt
    10081 agaagctgaa aaagggatct caaaagaaat cgtgatcgat gctttagaag cagcattggt
    10141 ttctgcatac aaacgtcact atggccaagc acaaaacgtc gaagtggaat ttgatcagaa
    10201 aaaaggaaag atccatgtct atgcggtaaa agaagtaacg gaagaagtga tggattcaca
    10261 gttggaagtc tcattgaaag atgcactttt aatcaatcca gcatatgaga tcggtgatac
    10321 gatccgtttt gaagtaacac caaaagattt tggacggatc gctgcgcaaa cggctaaaca
    10381 agttatttta cagcgtgtac gtgaagcaga aagaacgatt atttataacg aattcagtgc
    10441 ttatgaaaaa gacatcatgc aaggaatcgt cgaacgccag gataaacgtt atatttatgt
    10501 caatcttggt aaaattgagg cagttctttc taaacaagat caaatgccaa atgaatttta
    10561 tcaaccacat gatcgaatca aagtttatgt ttcccgagta gaaaatacgt ctaaaggtcc
    10621 gcaagtattt gttagccgca gccatccaga cctattgcgt cgcttatttg aacaagaagt
    10681 accagaagtc tatgatggaa tcgttgaaat tgtcagcatc gcccgtgaag ctggcgatcg
    10741 ttcaaaagtg gcagttcgct caaatgatcc aaatatcgat gcagtcggta catgtgtcgg
    10801 gccaaaggga caacgagttc aagcaatcgt caatgaatta aaaggcgaaa acatggatat
    10861 cgtcgaatgg aatgaagatc cagctgtatt catcgcaaat gcattgaatc ctgcacaagt
    10921 catggatgtg atcttcgatg agacaaatcc aaaggcctgc acagttgtcg ttccagacta
    10981 ccaattatcg ctagcaatcg gtaaacgcgg acaaaatgca cgtttagctg ctaaattgac
    11041 taaccataaa atcgatatca aatcagaatc tgacatgacg gaattttatg aaaatcaaga
    11101 acaacaaaaa gaaacagaag aactgcatga tgaagcaatc gttcagtcag atttaacaga
    11161 cgatgaatat gaaacaattg cattcaacaa tgaaacggtc gaagaaaaac cagaagctta
    11221 actttagtaa ggaggcaaag agatgaaaca aaggaaaatt cctttacgca aatccgtggt
    11281 ttcaggtgaa atgaaaccta aaaaagagat ggtccgaatc acccgttcaa aagaagggga
    11341 agtatcgatc gatccaactg gaaaaatgcc tggacgagga gcgtatgtct cacttgagcc
    11401 ggaagaagta caacaagctt gggataaaaa aattctcgat cgagtactcg aaacaacatt
    11461 aacagatgag ttttatcaag aacttcttga ctatgtcact caccaaaaag cacgtaaaga
    11521 gttatttgga aaatgaatac agttaatcgg caaaaagcca tgaatctcat cggtctagcg
    11581 atgcgcgcgg gaaaactaat cacgggtgaa gaactgacca ttgctgaaat ccgcaaacaa
    11641 aaagcaaaat tggtgtttgt ggcaaccgat gcaagtgaga atacaagaaa aaagataaaa
    11701 gataagagtt catattatga agttccttgc tttgagctat tttctgaagc agaaatcacg
    11761 cagatgatcg gaaaacctcg caaggtattc ggaataatgg atgccggatt tgcgaagaag
    11821 accaaggagc taattgaagg ttaggaaggt gattgcatgg gcaacaaaag aatctatgaa
    11881 ttagcaaaag aactcaaaaa atctagtaaa gacgtcgtgg aaaaagcaca acaacttggc
    11941 atcgatgtga aaaaccatat gggtgcgatt actagcagtg atgagaagaa attgcaacaa
    12001 gcgttcaaaa acccaagcac gaaacaacaa gctgcacaac aagcaagcag aaaaccagcc
    12061 aatcaacagc cgaacgatca acgtaaaaaa gaaacaacga acaatcaaca aaaaaataaa
    12121 aataaccgca gttatcaaga tcgcggtcaa gggagcggtc aagtgaatca aggaaaaaaa
    12181 ccaacaacaa accaaaataa tacgggaaat accagcagcc aaaatcgtca aggaacgagc
    12241 caaggcggca atcaaaaccg tactaacaat aacaacaaac caaacaataa caatcgcggc
    12301 aaatttaata ataataaccg taatcgttat aataaaaaag gtaaaaaagg caaacagcaa
    12361 caatcaacta aaccagctgt accaccacgc aaattccgtg aattgccaga tgtattagag
    12421 tatactgaag gtatgaacgt agcagatatt gcgaaaaaaa ttcatcgtga gccagcagaa
    12481 atcattaaaa aattatttat gcttggtgtc atggtcaatc aaaaccaagc actggataaa
    12541 gatacgattg aactgttagc gacggactat ggcatggaac cacaagaaaa agtccaagtt
    12601 gacattgctg atatcgataa attcttcgag gcagaagaag tcgatccaga taaattagtc
    12661 tcacgtccgc cagtagtaac gattatggga cacgtagacc acgggaaaac aaccttattg
    12721 gatacattgc gccattcacg tgtaacaagc ggcgaagccg gcggaatcac acagcatatc
    12781 ggtgcctacc agatcgatat tgatggtaaa ccaatcactt tcttggatac accaggacat
    12841 gcggccttta caagtatgcg tgcacgtgga gcaagtatca cagatatcac gatcttagtt
    12901 gttgctgcag atgatggcgt catgccacaa acagttgaag caatcaacca tgccaaagct
    12961 gcaggcgttc caatcatcgt cgctgtaaat aaaattgata aaccaggtgc aaatcctcag
    13021 catgttatgc aagaattaag tgagtatgaa ttaattcctg aagcatgggg tggagaaacg
    13081 atttttgttg agatctcagc gaaattcggc caaaacatcg aagaattgct ggaaatgatc
    13141 ctgttagtcg cagaagtaga agatttaaaa gcagatccaa cacaacgcgc aatcggaaca
    13201 gtgatcgaag cacgtcttga taaaggtaaa ggtcctgtgt caaccttact tgtccaagaa
    13261 ggaacacttc acgttggtga cccaatcgtt gtcggtaata cttacggacg tgtccgtgta
    13321 atgacaaacg atcttggccg tcgcgataaa gaagcaggac ctgcaacgcc agtagaaatc
    13381 actggcttga atgatgtacc tcaagcaggt gaccgtttcg tagtcttcga agacgaaaag
    13441 acagcacgtg cagcgggtga agaacgcgca aaacgtgcat tattggaaaa ccgtgctgcc
    13501 acaagccgtg tgactttgga caacttattt gaaagcctga aagaaggcga attgaaagaa
    13561 gttaacgtaa tcatcaaagc agatgtacaa ggttcagcag aagcactggc tgcatcattg
    13621 aagaagattg atgtggaagg tgtccgtgta aaaatcgtcc attcagctgt cggagcaatc
    13681 aatgaaagtg atgtaacctt agcagcagcc agcaacgcta ttatcatcgg tttcaacgtt
    13741 cgtcctacac cgcaggcaaa agtgcaagct gatactgaag aagtcgatat tcgtcttcac
    13801 cgtatcatct ataaagccat cgaagaaatc gaaactgcga tgaaagggat gctagaccct
    13861 gaatttgaag aaaaaatcac cggtcaaatg atcgttcgtg aaaccttcaa agtttctaaa
    13921 gttggtacga ttgcaggtgc ctatgtaaca gaaggatata ttcgtagaga tagcggtgtc
    13981 cgtgtaatcc gtgacggaat cgtaatctat gaaggccaac ttgcaagtct gaaacgattc
    14041 aaagatgacg tgaaagaagt caaaatgggt tatgaatgtg gtgcgatgat tgaaaaattc
    14101 aatgacatca aagtagatga cgtaattgaa ggatttatca tggaagaagt caaaaatgac
    14161 taaaaactga taaaaggagg ccaatcatta tggctaacta ccgtgatcgc cgagtcggtc
    14221 aagaaatctt aaaagaagtc aatgatattt tgcgcaaaaa ggtccgtgac cctcgtgtgg
    14281 agaacgtaac gatcacagat gtgaaagtta cgggggactt acagcaagca acgatctatt
    14341 acagcatttt atctgactta gcatcagata agaaaaaagc acaagatgga ttgaataaag
    14401 catcaggatt gattcgtcga gagttagggc acaatctaag tatttataag acacctgaat
    14461 tgttctttga acttgatgaa tcagttgtct atgggaacca tatcgatgaa ttgcttcgcg
    14521 acttgaacaa agactaatca aaaaatcccg caaatcctgt gaaatcaggg gttgcgggat
    14581 ttttttgttt tcattaagac gtattaccta agagttccat gactttagtc tggtagcgtg
    14641 tcagctaacc aaaaaagaga aaaagaaaag taaaaaattc gactttcttt tttctatagt
    14701 atataaattt ttgcatatag ataaagatca agataccgat aacggaagct agattgtaat
    14761 ctattttagt gacagtcagg tatagccaag taactagaag atcagtggaa cctgcaccat
    14821 aaaagtttga gtttgtcggt ccgttgacag ttaaaagaaa aatcacgttg aagttattga
    14881 tatttccgat tacgccgctt cgatttggtc acgatccaaa ttttggataa tccctgtaga
    14941 aattagcata gtagctggga taccaattca catattgaca aagataacag agacttttac
    15001 ataaaacgga tcaatcagga aaggaattgc tcgatccagc cccagttttc caacaatgtg
    15061 ttaactggcc tagcgtcatt cagcaaatta tgcatgatga ggagcgaaat gaactgcgga
    15121 acagccattg tgataacgac aagttttttg gatcaatgta aaaaacgtca gcgaaaggaa
    15181 aaatttcgtg gttttttata cctgttcatt tggctagata taaaatccaa acaaatgaac
    15241 agaagtgctt attaggtttc aattgggagt atctgcgcta gaataagagt aataatgaaa
    15301 aggagtgtta tgtatggatt attctagtaa agaatacttt gataaaatga cggcttggtg
    15361 gcgtgctgct aattatcttt ctgttgggca actttatctt aaagataacc ccttactgag
    15421 acgtacactg aaaccagaag atgtcaaaaa acatccaatc ggtcattggg gaacgattcc
    15481 tggccaaaat tttatttatg tccatttgaa ccgagtcatc aataaatatg atttgaacat
    15541 gttttatatc gaagggccag gacacggcgg acaagtcatg gtatcgaatg cttatttaga
    15601 tggaagttac acagagatct atccagaagt aacggaagat gagacaggaa tgcaaaaatt
    15661 attcaagcga ttttcatttc ctggaggcat tgcttctcat gcagcaccag aaacaccagg
    15721 ttctatccat gaaggcggag agcttggata ctctctttca catgcagtcg gagctgtatt
    15781 agacaatcct gaagtaatct ctgctgtcgt gattggtgat ggtgaagcag aaaccggtcc
    15841 tttagctgga agctggttct caaatgtctt tatcaatcca gtgatagatg gagctgtttt
    15901 accgatcctt catttaaatg gtgcaaaaat agccaaccca acgatcttgg cacgtaaatc
    15961 agatggagaa ttagcaaatt actttaacgg acttggctgg gagcctttct ttattgaagg
    16021 aaatgatcca gaaaaattaa atcctgttat ggctgaaaaa atggatcaag caatcgagaa
    16081 aatcaaatcc atccaaaaag aagctcgcct gaagacagca acagatgtcg tgatgccaaa
    16141 atggccagta ttgatcgtac gcacaccaaa aggatggacc ggtccaaaag aatgggacgg
    16201 ggaaccaatt gaaggaacct tccgagcaca ccaagtaccg attcctgtag atcaagaaca
    16261 tatggatcat gcagatgctt tactccgttg gctgaaatcc tatgaaccag aaaaattatt
    16321 tgatgcacaa ggacgtattt tagaagaaat cagagaaatt gcgcctactg gtgaccaaag
    16381 aatggcaaaa aatcctatca ccaatggtgg aatcgatcca agaccgctta ttatgccaga
    16441 ttggaaaaaa tatactttgc aatttgaaaa accaggctcc gttaaagcag aagatatgac
    16501 cgaacttggt aaattcgttc gcgagatcat tgagaaaaat ccagaaaatt tccgtatatt
    16561 tggaccagat gaaacaaaat caaaccgatt gaatcaagtt ttcaaaacga caaatcgcca
    16621 atggatggaa aaaatcgaac cagaaaatga tgaatggctt tcgccatctg gtcgcgtcat
    16681 cgacagtcaa ttatcggaac accaagatga agggttctta gaagggtacg ttctgactgg
    16741 acggcatggt ttttttgcaa gttacgaatc attcttgcga gtggttgact ctatgttgac
    16801 tcagcacttc aaatggatac ggaaatcaca tgacctttct tggcgaaaag attatccttc
    16861 gctgaatctc attgcaagct caacagtctt ccagcaagat cacaatggat atagccacca
    16921 agatccagga atattgacac atttggcaga aaagaaggcg gagttcatcc gtgaatatct
    16981 gcctgcggat gcaaatacat tgctcgcagt catggataaa gcattccgct cgtcagaaaa
    17041 aatcaatttg atcatctctt ccaaacatcc gcgtgctcaa ttttactcag cagaagaagc
    17101 agccgtctta gtgaatgaag gcttgaaaat cattgattgg gcttcgacag caaaagagga
    17161 ggaacctgaa ttggtcattg ctgctgcagg aacggaatct aacttagaag cattagcagc
    17221 tgtaactttg ctgctggaag aattcccaaa actaaaaatt cggttcatca atgtcgtaga
    17281 tttattgaaa ttacgtcatc caagtcaaga tccgcgtggg ttgagcgatg aagaatttga
    17341 tcaatatttt acaaaagaca aaccgatctt atttgctttc cacggttatg aaacattggt
    17401 ccgcacgatt ttctttgatc gtcataacca tcatttaatg attcatggct acaaagaaaa
    17461 tggagatatc acgacaccat ttgacatgcg tgtagtaaat gaacttgatc gttatcattt
    17521 ggcaaaagac gcagctttaa aaatcaaagg aagtcaagca gaagattttg cggaaaaaat
    17581 ggatcagaaa ttacaagaac atcaaaacta tattcgtgaa aatggtattg atctgccaga
    17641 agtattggat tggaaatgga aaaatcttga ccaataaaat gataaaatcg atcccgcaaa
    17701 acagaatttt ttacattgta ttatttgtca aattaag
//
LOCUS       DAZUMK010000048        17113 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000048 DAZUMK010000000
VERSION     DAZUMK010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.049"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..981)
                     /locus_tag="ACG808_001543"
     CDS             complement(34..981)
                     /locus_tag="ACG808_001543"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS5047172.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            1227..1691
                     /locus_tag="ACG808_001544"
     CDS             1227..1691
                     /locus_tag="ACG808_001544"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFS5047173.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(1753..2796)
                     /locus_tag="ACG808_001545"
     CDS             complement(1753..2796)
                     /locus_tag="ACG808_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFS5047174.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(3173..4138)
                     /locus_tag="ACG808_001546"
     CDS             complement(3173..4138)
                     /locus_tag="ACG808_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFS5047175.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            4372..5217
                     /locus_tag="ACG808_001547"
     CDS             4372..5217
                     /locus_tag="ACG808_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS5047176.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            5408..6595
                     /locus_tag="ACG808_001548"
     CDS             5408..6595
                     /locus_tag="ACG808_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFS5047177.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            6601..6957
                     /locus_tag="ACG808_001549"
     CDS             6601..6957
                     /locus_tag="ACG808_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFS5047178.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            6959..7297
                     /locus_tag="ACG808_001550"
     CDS             6959..7297
                     /locus_tag="ACG808_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFS5047179.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    7373..7629
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            7692..8726
                     /locus_tag="ACG808_001551"
     CDS             7692..8726
                     /locus_tag="ACG808_001551"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFS5047180.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            8719..9405
                     /locus_tag="ACG808_001552"
     CDS             8719..9405
                     /locus_tag="ACG808_001552"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFS5047181.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            9429..10277
                     /locus_tag="ACG808_001553"
     CDS             9429..10277
                     /locus_tag="ACG808_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS5047182.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            10474..11247
                     /gene="sufC"
                     /locus_tag="ACG808_001554"
     CDS             10474..11247
                     /gene="sufC"
                     /locus_tag="ACG808_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFS5047183.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            11260..12546
                     /gene="sufD"
                     /locus_tag="ACG808_001555"
     CDS             11260..12546
                     /gene="sufD"
                     /locus_tag="ACG808_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFS5047184.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIVVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            12543..13778
                     /locus_tag="ACG808_001556"
     CDS             12543..13778
                     /locus_tag="ACG808_001556"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFS5047185.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            13765..14235
                     /gene="sufU"
                     /locus_tag="ACG808_001557"
     CDS             13765..14235
                     /gene="sufU"
                     /locus_tag="ACG808_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFS5047186.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            14240..15631
                     /gene="sufB"
                     /locus_tag="ACG808_001558"
     CDS             14240..15631
                     /gene="sufB"
                     /locus_tag="ACG808_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFS5047187.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKAVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(15726..16868)
                     /locus_tag="ACG808_001559"
     CDS             complement(15726..16868)
                     /locus_tag="ACG808_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5047188.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(16907..>17113)
                     /locus_tag="ACG808_001560"
     CDS             complement(16907..>17113)
                     /locus_tag="ACG808_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047189.1"
                     /translation="NNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDNIN
                     KLEQTISSIGKKYTKKKMKGGDTI"
BASE COUNT         5564 a         3224 c         3603 g         4722 t
ORIGIN      
        1 atttaaaaaa atgtttactt catttgacgt atattattgg tgaggcttca attcatttgg
       61 cttcactttt tctgtttctc ttatgatata aacaggacgc tgtttcgttt ctaagaagat
      121 tttcccaata tatttcccga tgattccaag acataacaat tggattccac caatgaacag
      181 gaagatcgag accatggaag gccatcctga tgtaggatca ccaaacaaca atgcccgcaa
      241 aataatgaac aaaattgcaa taccagaacc gacacaagac aaagcgccga taaatgaagc
      301 aatcgttaac ggtgcatccg agaagttcac gataccatca atcgagtagt tgaataattt
      361 ccagaaggac caagatgttt cgccagccac acgttcgcgg ttttcataag aaatatattc
      421 tgttttaaat ccgacccagc taaataatcc ttttgaaaat cggttgtatt ctgttaattc
      481 taggacggca tccaccattt gacgagtcat caagcggaaa tcacgagcgc catcgaccat
      541 ttctgtatcg ctgattttat tgatcaactg gtagaatttt ttcgcaaaga aactgcgtat
      601 cgcaggctcg ccttcacgag tcgaacgccg ggtgccgaca caatcgatat cagaagtagt
      661 gatccggttg atcatctcag gaagcagttc gggcggatct tgcagatcaa catccatcac
      721 ggtcactaaa tctcctgtag aagcttgcaa tccagcgtac agagccgctt ctttcccaaa
      781 atttctggaa aatgagacaa agcggacata aggataagct gaggctagct tcttgattgc
      841 tgctaacgta ccgtcttttg atccgtcgtc aataaacaaa tattcgaact catgggcacc
      901 tttgaatttt tcaacctcat caaaaaaaag aggaacagtt ttttcttcat taaaacatgg
      961 gacaacaatt gatactttca tcgtttctat cctttcaatc gtatgtttat atatggttta
     1021 cattcaaaat ttaataaacc ttaaataaca cctttaaaat ttgattttac gtaaaagaac
     1081 aggtattctt tttgtaaact aacgtattta gtttaccatg tttcctcggt ttatcaaact
     1141 tttaagaaca atttttatta gttggcaagt agttttggat agagaaaaag gaggaagttc
     1201 cagtctattg actaggagca aacaaaatga aaatacacac acctatcggc tttttatggt
     1261 tagatgcaga tgaatccgga ttgactgctg tatcttttca gccaatttca gaaagcgaat
     1321 cagcgaatca gcgaatttta acgcgcacac aagaacagtt tgaagaatat tttgcgggaa
     1381 aaagaaaaga atttgatgtt cctttatcta taaagagagg tacgcctttt cagcaaaatg
     1441 tctggaaagc tttgtcgacc attccttatg gagaaatccg aacgtataaa gaagtagccg
     1501 ttgctgttga tagtccgaaa gctgtgcgag cgattgggca ggcaaatcga accaatccat
     1561 tgccaatcgt tattccttgt catcgagtga tcgggcaaaa tggaaaactg acaggatata
     1621 tgggaaacgc aatagatggg gttgaaatca aacgccaatt gttgttgtta gaaggatatc
     1681 tgcaaaatta aaaacagttg tgattcggac attgttccat gcgagaatca caactgtttt
     1741 ttcatgttca ttctaaactt tgatcagcca aataaatagt aaagccatca ctgtttggag
     1801 aagcccgaca gctaatccat aagataagaa gcgtgtacct tctttcatga attttgcaaa
     1861 gtctaaacgc agaccaattg ccgctaaagc agtgatctca aaccagctgc tgataaaatg
     1921 tgccgattgg tcaataacta caggtaaaga aaacaagctg ttgaccaaac aaaagatcac
     1981 aaaccctaat acataccaag gaagcggtaa ttttcgtttt ttctgcgtat cagttggcgt
     2041 ttcaaacaga gctggttgtt tcttgtgcat gaatttttca aacagataga caacaacaac
     2101 gagcatcata atacgtagaa tcttaaatag catggcgaat tcgacaacag aattgtcaag
     2161 taagcttgcc ccagcgacta cttgaccaac agattggagt gtaccaccca aaagtgcact
     2221 tttttcaatg agatttccac caaacaactg taatccaaga aaagggagag tcaacatcat
     2281 cacagtaccc agtagattga ctagtgtgat gatctgtcct ttttcttcct ctttcgcatg
     2341 gatggaaggg gcgattgaag caatagcaga agaaccacaa acagcgtttc ctccagccat
     2401 catcaaagaa gaagtatcgc tgaattgcag ttttttacca atcaagtaag caccgatgat
     2461 agtgactgac atttgcagta acacaaaaac aattcctttt acaccaattt gagcaatcgt
     2521 ttgagctgtg acagttgcac ctagtaaaaa aacagagaat tctaatagtt tgctttcagc
     2581 aaattttgtg ccgattgcta gaacaggctg tttaaaaaaa gtatttccta ataaaatacc
     2641 caataaaata gcaatagtcg ctccacctaa ctgtggcaga taaatagcaa gaagcctgct
     2701 gaaggcagcg atggcaaaag aaagcagtaa accgggtaag atttcgcgga ccgctgaatc
     2761 ctttacagat tttattggat gtgacaatga tttcatttga tcggttcctt tcgtcaataa
     2821 atactaaaag catactcgaa catagtagca ctaaaccagt attttgacaa aagaacaaga
     2881 ggtcgtgaca aaagtcttgt acgacctctt gtttcgaata aacggtgttc cagaagtagc
     2941 ttctacggaa ataagccgaa atttcacaaa aatttgagaa gcaattttcg taaattcctt
     3001 cttatttctt gaagctgacc acttctttta cgacctcttg gtaaacggtg ttcaaaagct
     3061 tgcatctgcg gtaataagcc caaccagtac aaaaacatga acagctgttt ttgtttggcc
     3121 gttcttatta cttgatgcag aacagctttt tcacaaccta ttgaatcgat tattaagcga
     3181 tttttttaga aacaaatttt ccatgtttgc tatgttcttt aattaagaaa cgtacgtaat
     3241 gagcaagaac gtagaagaca actacacttg caaacagaca ttccgcaact tttaagaaat
     3301 ccaatacgct actatagata cctaaactgt tgaagtaatg aacagtagta aatatagctg
     3361 ttgcagagat aacaagcggg aatgtaaagg ctgcatagct tggataaaaa cgaagcttga
     3421 acaaacgtgg aagcatagac agcacaacga aatacaatac ttgagataaa gcgaacaatg
     3481 ctacgacaag ataaaaatta ggttcagcaa agtttttaag ataacctgtt aggcaaagag
     3541 aaccaggtgc tgcaacgatc gtaatcagcg gacgtgtcgg atgagccata tttcttacga
     3601 aaaagacacg tttgatgatg atcggcaata agaacgcata gcagatcaat gaaatccaaa
     3661 agaaaatttt tccaataagt gggaagaagt tcccggcagt tacagtgatg accccaaatc
     3721 caacgtaaat gataaaccag cttggataaa ttgcttcaat tgaaagatca gctttgataa
     3781 catggtgata attgaaataa aagacaagga tcacttggaa gataacagcg cacagccaaa
     3841 tgaatttgac aattggtgca atactttcat atgaaacaaa ataagtacaa ataaccatga
     3901 gtgacatcgt aaaagttggt gaaacggagg cgatgattgg atcatgcaac gtttgtttcg
     3961 tgtgttcgaa taagataaac aatttaacaa aaatgaatat cataagaatc atagagatac
     4021 cgccagttag attgcctaaa gcaacatggt gatagtcttt aaacagattt ccaagggaag
     4081 ctaacccaag gatcaacccg cagatcggga tcggaataat tttcaaaaag ttcttcatat
     4141 ataatattct cctttttatt taatagattt tagtaccaag atgaacgaat acattgtaaa
     4201 atgaatagga atgtatgatt acgagcaagc agacagctga ttgtcttcat ctaagttact
     4261 agttggatca gcaactgttt tgttgtacat ttctattata gatggttttt tgtatattta
     4321 aatcaaatag tttttatagt aattatcata aaaagttatg gaagtggtgc catgtttaag
     4381 ttattaacga cttttcgagc agtatatgaa acaagaaatt tttccagagc agcagaagta
     4441 ctgtttttat ctcaaccagc agtctcgaat caaatcaaac agttggagcg ggaattgaat
     4501 atcgatcttt ttcaacgtaa cggaagaaaa gagatgcttc caactaagca agccgactta
     4561 ttatatgaac ggacactggt tttgttagaa gaatgggaag aaactcagca aaaattgctc
     4621 aatgaacaaa atgaagaaga agtatgtcgg atcgttgctt cccacacctt tgctgtctat
     4681 cttttgcctc agttgatgaa acatctgatc aaaaggtttc ctaaagtgca ttttgagatc
     4741 gaaatcacga attctcatga atcattagaa caagtagcga aacatgaatc cgattttggt
     4801 tttatcgaaa agccattaga gacttccggc gtacaaagat actctttgat ggaagaccag
     4861 ctagtcatcg ctggggaccc aaaaagcaaa ttgtggcttg taagagaagc aaactctggt
     4921 gtttttcatt acacgcagcg ttattttgaa gagatgaata tccaagataa gaagatgatc
     4981 gtgaaaagta acgatgtaat tgttgcgttg ctgaaggaag gaatcggtcg ctcgattttg
     5041 tctaagcgct ctgttccaga aaatgtgcca atgactccgt tgaatttcca tcgatatttt
     5101 tatttcatcc aacgaaccaa tctggtttct gatcagctgc tagagattgc cgaagagatc
     5161 cttcgataca atgaaagcac agaaacttct cacagcgagg agaatctcag ctggtagata
     5221 attgcatgta tcaaaaaaag ttctatgata cactaaagat aagcagtagg cttttgtact
     5281 gcatttgctc aagcaactgc acaaacggaa agttaatttt tcccaaatgt ctatggaaag
     5341 actttccatt atctaactgg accgctataa taatcagcag tatggttttt aagaaaaggg
     5401 atgaaacatg gaaaaaaaat taaaagcttc gaacgataat cgtattgatt atggcgtgat
     5461 tttaccagtc tttctgttat gtttgattgg tctgctctca ttatatgtag cacttagcca
     5521 tgatccgaac aatccagcag tcttgaaagg cgtagggatg caagttgtct ggtatttagt
     5581 tggagcggca gcaatcgttg tcatcatgca tttgaattcc aaatggatct ggaaactgac
     5641 tccttacctt tatggtttgg ggttagtggt aatgggactc ttgctcaaat tctatgatca
     5701 gaatttagct gcttcaacag ggtctaaaaa ctggtttcga ttcggtgcgt ttactttcca
     5761 gccagcagag ttgatgaaga ttgcgtacat cttgatgatg gcactgatcg taactaaaca
     5821 taatacgcaa gtaaaagaac ggacgatgaa atcggacttt tggctgattg ggaagctctt
     5881 gatcgtgaca ataccggttt tagcattgat catggcccaa gatgactttg gaacgatgct
     5941 tgtctttctt gcgatctttg gcgggatttt cctgatgtct ggaatctctt ggcgcatcat
     6001 cgcacctgta gtaatcttgg cagtagtcgt tggagcagga acgatcttct tggttacgac
     6061 tgaaggcgga agagacttgc tttataaagt tggattcaaa tcttatcagt ttgcacgtat
     6121 tgattcatgg ctggaccctt tccatgatac gtcaggaatg agttatcagc cggcccaagg
     6181 acttttggca attggtaccg gtggcttgtt tggtaaagga ttcaacgtaa gcaatatcta
     6241 tgtgcctgtt cgtgagtccg atatgatttt taccgttatc ggtgaaaact tcggctttat
     6301 cggtggtgca ttcgtcattt tcttgtattt tattttaatc tatcgtatga ttcgggtgtg
     6361 tttcgatacg aataatgaat tttacgccta tatcgcaagc ggtttgatca tgatgctgct
     6421 gttccatgtc tttgagaata ttggagcaaa tatcggatta cttcctttaa cagggatccc
     6481 attgcctttc attagtcagg gaggatcctc gattttaggg aacatgatcg gtatcgggtt
     6541 gatattatcg atgagatacc aaaatgaagc acctgcaaga tcttcaagga ggtaagaaaa
     6601 ttgtacacct tttactggta cccgaaatgt tcaacatgca aaaaagccaa agcgtggtta
     6661 gaccagcatg gcgttagtta tcaaaccgtt gatatgatca aagaagtacc gaaggcctca
     6721 acgattgaga cttggctaga aacgagtcaa gtaccaatcc gtcgcctgtt caacacaagc
     6781 gggatgaaat atcgggaact tggactgaaa gatcaagtag gcgagtttac ggcttcaaaa
     6841 gcgagccaag tcttatcgac agatggtatg ctgatcaagc gaccactgat cttacgagag
     6901 gaccagcttg ttgcaattgg attcaatgaa aaaatttatg aaggagaact gatataaaat
     6961 ggcaggcaag catttgaaga aaaaggacaa cctttggatc ttatttaatg gaaaagaata
     7021 ctgtgttggt ttgacaaatg aagcgcaaga agaacttgga gacatcacgt ttgccaatct
     7081 gccaaaagca ggacaatcgc taaaaaaaga tgaacctctg atcgaagttg aagcggaaaa
     7141 agcggtcaat gaatttgcca gcccgctaac gggtacagtt tcttctgtta atgataaaat
     7201 cggtgaagat atcaatatct tgaatgattc agatgaaatg aatgcgtgga tcttaagttt
     7261 caaagatgtt gatccaaaag aatttgacga attataaaat cagaaaatca cttgaaaaaa
     7321 gttgacactg attcttccag ttgctataat cataagcaat cctatatgaa acgagaaagc
     7381 tttgaaaaga agagtacatg tcacggacgt ttgaagagag ccctgtttgc tgaaaatggg
     7441 taacgaaaat gagatggaag atggtcttag agcagagtaa gcgagcgtaa gcaaacttac
     7501 tacgggagtg cccgttacag cgcgacagta tacggaaaga agctaagtcc aagtactgga
     7561 agaggctgtt attgcgaagt aacggtaaat caaggtggta acacggaagt tcaagctttc
     7621 gtccttttgc ttaaggcaag agggcgaagg cttttttgtt tgaaaaatca taagagaaag
     7681 atgtgaatgg catgtcatta atccaactaa cgaatgtcaa aaagacattc aatgggaaaa
     7741 acggaaacat ccaagccgtc aatgacgttt cactaaacgt tgaaaaaggg gatatttatg
     7801 gaatcgtcgg ctattctggc gcaggaaaaa gtacattagt ccgtctcctg aatggacttg
     7861 agcttcccac gagcggcgaa gtgatcgtaa acgatcaaga tattacaaaa ttaaaaaaca
     7921 aagaattacg tacattccga aagaaaatcg gtatgatctt ccagcatttc aacctgttat
     7981 ggtcaagaac tgtacttgaa aatatccagc tgccgctgga attagctggc gtaccaaaga
     8041 gcaaacgtaa agaacgggca gaagaactgt tgcgtttagt cgggttagaa ggacgcggac
     8101 aagcatatcc ttcacaactt tccggcggac agaaacaacg cgtcggtatc gcacgagcat
     8161 tggcaaatga tcctgagatc ctgctttgtg atgaggctac tagcgcattg gacccgcaaa
     8221 caacagaaga agtattagac ttattgttag cgatcaataa gaaattgaat ttgaccatcg
     8281 tcttgatcac acatgaaatg aatgtgatcc gcaaaatctg caacaaagtc gctgtgatgg
     8341 agttggggaa agtcgtagaa gaaggagacg tattgactgt cttcagacaa ccgaaaaaag
     8401 aagtaaccaa acgctttgta caacaagaca tcgaaccaga agctgattcg acagaactat
     8461 tagctgaact catcaaagaa aatccaagcg gccgtttggc aactttgacc tttagcgaaa
     8521 gaaatgccaa cgagccagtc atttcccaag cgattcgacg ctataacatc gatgtcaacg
     8581 ttgtgtatgg aaagatcaaa caagtgaaag aaggttcctt tggttcattg actgtcttga
     8641 tgactggtga aaccaaagaa ttagatcaag cagaagtgtt catcaaagaa caaggagtag
     8701 gaattgaggt gatccatcgt ggataaaagc ttttcagaaa catatctaga ttttaaacaa
     8761 atcaacccgg aaacgatgaa tcaggctatc cttgatacac tatatatgac attaatctca
     8821 atggtcttag tgacgatcat cggattagtt ttgggattga tcctttactc tattggacgc
     8881 aaaaacaaac cgtatgctcg aatcatttat ggaatcgtat cgatcatcag taatattttc
     8941 cggtcaacgc catttatgat cctgatggtt ttgatcattc catttactaa agcaatcgta
     9001 ggcactatgc taggtgcgca agcagccatt ccagctcttg ttttatccgc agcaccattt
     9061 tatgcccgtc tagtagagat tgcttttcgt gaagtcagcc ctggtgtact ggaagcagca
     9121 gatgcaatgg gagcaagtta ctgggaaatc atctggaagg tcttgatacc tgaaagtatt
     9181 cccgcattga tttctggatt aacagttaca acgatctcga tgatcggatt tactgcgatg
     9241 gccggcgcga tcggcgcagg tggacttgga ggtcttgctt ggcaggaagg ctaccagcgt
     9301 ggtaatctaa cagtgacatt cgttgcgact ttaattattt tactaatcgt atttatcgtt
     9361 caaggaatcg gtgatttctt aactaagaaa acagacaaaa gataatagga tataggggga
     9421 tgtaggatat gaaaaagaaa atttttggat ttgcagcagc gcttttgctg actgtaggat
     9481 tagcagcatg cggtaacaac agcgacagca aagattcagc aaataaagca gacgacacga
     9541 cattgaaagt cggcgcttca ccgacaccgc acgcagagat tttggaacat gtgaaaccat
     9601 tattgaaaga tgaaggaatc gatcttgaaa tcgtcaaatt cgatgactat gtattaccga
     9661 accaatcttt ggaagaaggc gacattgatg ccaattactt ccagcatatt ccttatttga
     9721 acaaagaaat caaagaaaaa ggatatgact ttgtcaatgc cggtgctgtc cacatcgaac
     9781 caatgggctt gtactctaaa aaagtcaaag atgtttccga attaaaagat ggtgcaacaa
     9841 tcattacttc aaattctgaa tcagactggg gacgcatcat cacaattcta caagatgctg
     9901 gcttagtcaa agtcaaagat ggagtagatc tggaaacagc aacattcgat gatattgaag
     9961 aaaaccctaa gaacttgaag ttcaaccata cgatcgaccc atcactttta gcttctacgt
    10021 accaaaatga tgaaggtgac ctagttgcaa tcaacgcaaa cttcgcttac ggcgcagggt
    10081 tgaatccatt agaagatgcc gtattgcttg aaaaggacaa ctctccttac gtgaatatta
    10141 ttgcgactcg taaaggtgat gaagacagtg acaaaatcaa aaaactgatt gaagtgcttc
    10201 accgcaaaga agtccaagaa tggataacag acaaatgggg cggctctgtt aaaccagtaa
    10261 aggctgatgc aaaataaggt atggaacaaa aaagatgaga aataagtctg taatggctta
    10321 tttctcatct tttttatacc tattctaatt tagaaatcaa aattgtttaa taataaataa
    10381 aagatatttg attttagtaa ggaaggtcat tgcgcttcat tctcaattag tctaaaatga
    10441 gtgagaacga aagagaattt gggggtataa tgcatgtctg tcttagaaat aaaaaatcta
    10501 catgtgtcga tcgaggaaaa agaaatcctt aaaggcgtaa acctaacaat gaaaaccgga
    10561 gaaatccatg ccatcatggg acctaacggc acaggaaaat ctaccttatc tgccgctatc
    10621 atgggcaatc caaactatga agtaacagaa ggcgaaattt tgtttgacgg gaaaaatgtt
    10681 ttagacttgg aagtagatga acgtgcgcga cttggcttat ttttagctat gcaatatcca
    10741 agtgagatac caggtatcac gaatgcggaa ttcatgcgtg cagcgatcaa tgcgaaacgt
    10801 gatgaaaacg acaaaatttc tgttatggca tttttgaaaa agttagatga aaaaatggct
    10861 ttactaaata tgccagaaga aatggctgaa cgttatctga acgaaggttt ttctggcgga
    10921 gaaaagaaac ggaatgagat cctgcaattg ttgatgttag aacctacatt tgcaatttta
    10981 gacgaaatcg attcaggtct ggatatcgat gctttgaaag tcgttgctaa aggagtaaat
    11041 gaaatgcgcg gagacaattt cggtgcattg atcatcaccc actatcaacg attattgaac
    11101 tatatcacac ctgatgtcgt tcacatcatg atggaaggcc gagtagtcat gacagggaat
    11161 gctgacctag ctaaacgtct ggaagcagaa ggctacgctg gtatcagtaa agaattagga
    11221 atcgactata aagaagaaga agcgtaaggg ggagcaagaa tgaaagaaaa aaactggctc
    11281 gattatcttg acgcggtaaa tgatttttct ttaagtaaag gcgaacctga ttggatgaga
    11341 accttccgtc aagacgcact agcgaaagca gatgaactgc cattgccgca tatcgatcgg
    11401 gtgaaattcc atcgctggtc gttatttgat gtgaaagaaa cacaaaccat cagtgaaaca
    11461 ggcacgattc cggcatttga tgcaatgaaa gacaatcctg tgcttgtcca acaaggttca
    11521 tggacgattt ttgaacaact gcctgtggaa ttagcagaaa aaggagtcat ttttaccgac
    11581 ttgttcactg caatgatcga atatccagaa ctagtccaag aatattatat gaaaaaagca
    11641 gtaaacatga acgaagacca attgactgca ctccatgtag cttttatgaa cagcggtatt
    11701 ttcctgtatg tgcctaaaaa tgtcgtgatc gatgaaccac tcgagtcctt attcattcaa
    11761 gatggcgcat ccgatgagca cttcttcaag catgtgttga tcgtggcaga tgaacatagt
    11821 gaattttctt atctggaacg tttccagaca accaaagaac aagtagcaaa atcatcaggt
    11881 aatatcatcg tggaagtcat cgctaaagct ggttcaaaaa tcaaatattc agccgtagat
    11941 caattaggag aaaatattac ttcttatatg aatcgccgtg ggcacatttt acgagatgct
    12001 tctgtcgact gggcaattgt tgtcatgaat gacggtcatg tgatcgctga ctttgactca
    12061 gatttagcag gagaaggtgc acacgcagaa gtcaaaatcg tggcaatcag ctcgggcaga
    12121 caaatccaag gaatcgatac acgagtaacc aataaagccc ctcatacgat cgggcatatt
    12181 ttgcaacacg gagtgatccg agaaaaagga acattgacct ttaatggtat cggccacatc
    12241 ttgaaagggg caaaaggtgc agatgcccaa caagaaagtc gtgtcttgat gctttctgat
    12301 aaagcacgtg gtgatgcgaa tcctattctt ttgatcgatg aaaacgaagt aactgctgga
    12361 catgctgcca gtgtaggacg agttgatcca gaagaaatgt actatctgat gagtagagga
    12421 ttgcataaag aagaagcaga gcgcttagtg attcgaggat tcctcggttc tgtcttaaca
    12481 gcaatccctg tagaacaagt tcggaaagaa ttagtcgaag taatcgaagg gaagttgaat
    12541 ggatgatcga tgctaaaaag ctacgcaatg attttcctat cttatttcaa gacgtcaatg
    12601 atgaaccttt agtttatctg gataatgcag cgacgaccca aaagccaaaa caagtactcc
    12661 aggcattagc gtactattac gaacacgata acgccaatgt tcatcgcggt gtccatacgt
    12721 tagcagaacg cgcgacccat gattatgaac aagcaagaga aaaagtacgg gcgttcatcc
    12781 atgcaaaaga aacagcagaa gtgctgttta ctcgtggtac gacgacaagc ttgaattggg
    12841 tagccagaac cttcggcgac cgctatatca aagcaggaga cgagatagtg ctctcttaca
    12901 tggaacatca ttctaatgtt atcccgtggc agcagttggc aaaacgaaca ggtgctgtct
    12961 tgaaatatat cgaactaaca gaagaaggct tcctggatat ggaggatgca gcgaaaaaga
    13021 ttacggacaa aaccaaaatc gtctccattg ctcatgtatc caatgtctta ggtgtcttca
    13081 atcctgttca agaattagct gaacttgtcc atgaaaacgg cggggtcatc gtagtagatg
    13141 gtgctcaatc tgccccgcac atggccattg atgtccaagc actagactgt gatttctttg
    13201 ctttcagcgg acacaaaatg tgcggaccaa caggtattgg tgttttatat ggtaaacgaa
    13261 aatggttaga agaaatggaa cctgttgaat ttggtggcga aatgatcgat tttgtcgaat
    13321 tatacgacag tacatggaaa gaactacctt ggaaattcga agcagggaca ccaaatatcg
    13381 ccggcgctat cgcattaggc catgcaatcg attatttgca aaaactcggt atggacaata
    13441 tccatcgata tgaagaagaa ctagccgctt atgtgttgcc aaaattacaa gcaattgatg
    13501 gattgactgt ttatggtcca caagacccga attttcggac aggagtcgtt gcattcaatt
    13561 tggatgggtt acatccccat gatgtcgcga cagctttaga catggaaggg gtagccgtca
    13621 gagcaggtca tcattgtgcg caaccgctat tgaagtacct tgaagtatca gcgactgctc
    13681 gcgcgagctt ttatttctac aacacaacgc aagacgccga ccgtttagtt gaagcaatca
    13741 tggcaacaaa ggagtttttc caacatggga ctatctaaat tagacaattt ataccgtcaa
    13801 gtgatcttgg atcattcttc ccatccgcat catcgtggaa gcttgactga ctcgacaagc
    13861 aagatcgaat tgaacaaccc cacatgtgga gatgtgatcg aattgcaagt cgatgtaaaa
    13921 gacggaatca tcaaaaatat cgcctttgac ggcagtggct gctccatcag tacggcaagt
    13981 gcctcgatga tgacagacgc cgtcatcgga aaatcaatag aagaagcaga acatttaact
    14041 gtagactttc tacagcttgt ccaaggaaaa gaagtcaaag atcccgacca attaggcgat
    14101 gctgctatgc ttggtggtgt agctaaattt cccgctcgga tcaaatgtgc gacacttgcc
    14161 tggaaagcac taggaaaagc aatggaaaac gatggtaccg cagctttcca tcattaccac
    14221 aaggaagagg agtgagaaca tgggcgtacc agaattagaa gaatataaat tcgggtttca
    14281 cgatgatgtc gaaccggttt tcagtacagg agaaggcttg acagaagaag tgatccgtga
    14341 gatgtcaaga atcaaaggcg aaccagaatg gatgcttgaa ttccgtctga aatctctaga
    14401 aacatttaac aaaatgccaa tgcaaacatg gggccccgac ctttcagaca tcgatttcga
    14461 tgccatcaaa tattaccaaa aaccaagtga caaacccgct cgtgactggg aagacgtacc
    14521 agaaaaaatc aaagatacat ttgaaaagat tggtatacca gaagctgaac gagcttatct
    14581 agccggtgct tccgcacaat atgaatcaga agtcgtgtac cataatatga aagatgaatt
    14641 cgaaaagtta ggaatcgtct ttactgacac tgactctgct ttgaaagaat accctgaatt
    14701 attcaaagag tacttttcaa aacttgtacc gccaaccgac aacaaattag cagcgctgaa
    14761 ttcagccgta tggtcaggtg ggacctttat ttacgtgcca aaagccgtca aagtcgatgt
    14821 tccattacaa acgtacttcc ggatcaatgc ggaaaacacg gggcaattcg aacgtacgct
    14881 gatcattgtt gacgaaggag caagcatcca ctatgtcgaa ggttgtacgg cacctactta
    14941 ttcaagtaac agcctgcatg ccgccatcgt agaaatcttt acaagaaaag atgcgtattg
    15001 ccgttacaca acgatccaaa actggtcaga caatgtgtac aacttagtaa ccaaacgagc
    15061 aaaagcatac gaaggcgcaa ccgttgaatg gatcgacgga aacctaggtg caaaaacaac
    15121 gatgaaatac ccaagtgttt acttagatgg aaaaggtgca cgaggcacca tgctctccat
    15181 cgcctttgca ggagcaaacc aaatccaaga tacgggtgca aaaatgatcc acaatgcacc
    15241 aaatacatct agctctatcg tctctaaatc aatttcaaaa gacggcggag aagtaaacta
    15301 tcgaggacaa gtaacctttg ccaaaaacag tgcgggttcg atctctcata tcgaatgtga
    15361 cacgatcatc atggacgaat ggtcgaaatc cgatacgatc ccattcaatg agatccacaa
    15421 cagccaagtc tctttagaac acgaagccaa agtctcaaaa atctcagaag aacaactcta
    15481 ctatctgatg agccgaggcc tgaccgaatc tgaagcaact gaaatgatcg tcatgggctt
    15541 tgttgaacca ttcaccaaag aacttccaat ggaatatgca gtcgaactca atcgattgat
    15601 cagttatgag atggaaggca gtgttgggta aacctgttat aggagctaga acccttgata
    15661 tataagggtt ctagcttttt tgttttgcct gtatactaga aatgaccaga ttttgaccag
    15721 ctcttttact gatgagccat catatcagca aagtcttgac ctgttttctt aggtgcatta
    15781 tttgtaatat gtgcatagat gtctaatgtc actttactat tttcatgccc taatcgttgt
    15841 tgaactacct tgacagattc tcctgcttct aataataaac tagcatgtgt atgtctaaac
    15901 ccatgaggtg taatacgagg caagttatat tttttcaaaa tatatcttaa ccaatcatta
    15961 acagtgtttg gaatgtatag agtgttttta actgttgtaa aaacatgttg ctctttagag
    16021 ctagtattgt atccaagttt taaatactct tctttttgct gtaatttcca gttgttgagg
    16081 atagaaagag tctcagtatc taaagagata actcgtctag aagctcttgt tttcggagat
    16141 tgaattataa ctttaccaaa ttcatctaca gcaagagttt tattgacatg tagttcttta
    16201 ttaaacgtgt ctatgtcttt ccactgtaaa gcgagtactt cactctttct catacctgtg
    16261 tatgccaata atctgaagaa ggcgtattgt ttcatgttac caaaatcttt aaaagcatcc
    16321 agtaaattat taagctcctc ttttgagtaa aatttacgat ttttctcata ctcttttttt
    16381 cgtggaagaa gtgttttttc cataggatta ttgtcaatat attgcattgc aaccccgtag
    16441 cgtaaaattt gtgcagtctg ttttctcata taatcgtaac tttcatattt actgtgccat
    16501 tcatctacca ctttttgaca atatgggaca gagatattcg atagtttaag cttaccaaat
    16561 gctggaagta tttgattatt tgcgtatctt actgagattg cgattgttga agctttaaca
    16621 ctggtacgct gatgagttat ccattcatta tacaggtctt caaatgtcat atcaggtgct
    16681 ttctttttaa caccgtataa tatttgatgt ctgtactcgt cataatcttt ttgagcttct
    16741 tgtttgctac taaaacctct tctcgtagta tttatttttt caccagttcg tggatcggtg
    16801 gcaatgtgtc ctttatacat ccacgccact tgaccgtttt tctttttgta ctttgatatt
    16861 tttgccatta aatcatctcc tcttagttta caaggagtac ttaccactag atggtatcac
    16921 ctcccttcat ttttttctta gtgtattttt tcccaattga tgaaatcgtt tgttctagct
    16981 tgtttatatt atcagtaatt tctctcacag cttcttcggg ataagacatg tctttcaatt
    17041 gaataggtag ccattcatct accattcttt ctagtagttc ttctatactg ctagtgagga
    17101 aaagaatgtt att
//
LOCUS       DAZUMK010000049        17024 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000049 DAZUMK010000000
VERSION     DAZUMK010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.050"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(16..561)
                     /locus_tag="ACG808_001561"
     CDS             complement(16..561)
                     /locus_tag="ACG808_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFS5047190.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(573..1265)
                     /locus_tag="ACG808_001562"
     CDS             complement(573..1265)
                     /locus_tag="ACG808_001562"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFS5047191.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1284..1568)
                     /gene="macP"
                     /locus_tag="ACG808_001563"
     CDS             complement(1284..1568)
                     /gene="macP"
                     /locus_tag="ACG808_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFS5047192.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1581..2144)
                     /locus_tag="ACG808_001564"
     CDS             complement(1581..2144)
                     /locus_tag="ACG808_001564"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFS5047193.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2372..3034
                     /locus_tag="ACG808_001565"
     CDS             2372..3034
                     /locus_tag="ACG808_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFS5047194.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3158..4354
                     /locus_tag="ACG808_001566"
     CDS             3158..4354
                     /locus_tag="ACG808_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFS5047195.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4455..5831)
                     /locus_tag="ACG808_001567"
     CDS             complement(4455..5831)
                     /locus_tag="ACG808_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS5047196.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5868..6734)
                     /locus_tag="ACG808_001568"
     CDS             complement(5868..6734)
                     /locus_tag="ACG808_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFS5047197.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6823..7848)
                     /locus_tag="ACG808_001569"
     CDS             complement(6823..7848)
                     /locus_tag="ACG808_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS5047198.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7866..8540)
                     /locus_tag="ACG808_001570"
     CDS             complement(7866..8540)
                     /locus_tag="ACG808_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5047199.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8724..9617)
                     /locus_tag="ACG808_001571"
     CDS             complement(8724..9617)
                     /locus_tag="ACG808_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS5047200.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9691..11388)
                     /locus_tag="ACG808_001572"
     CDS             complement(9691..11388)
                     /locus_tag="ACG808_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS5047201.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11381..12694)
                     /locus_tag="ACG808_001573"
     CDS             complement(11381..12694)
                     /locus_tag="ACG808_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFS5047202.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12694..16963)
                     /locus_tag="ACG808_001574"
                     /pseudo
     CDS             complement(12694..16963)
                     /locus_tag="ACG808_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333417.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
BASE COUNT         4789 a         3839 c         2953 g         5443 t
ORIGIN      
        1 aatcactttg tttttttata aaatttctgc gcctagtgtg gactcaaaat gtctcaatgc
       61 ccattcatgt ccaacgggat cgaaacttgc cacggcatct ttgaaaatat ggagtttgta
      121 tcctagatta tacgcatcga ctgctgtatg caacacacag atatctgtac atacgccggt
      181 caaataaata tctgtgatct ggcgttctcg caaacggatg tctagatctg ttccactaaa
      241 agcggaataa tgccgtttgt ctatccaata aacattttcc tcttgctcat agtcttggta
      301 taaacttttt aaagagccgt acaattcccg tccacccgtt cctatcacat tatgcggagg
      361 aaataagcgg ttctccggat gaaaggaatc ttttggatca tgcgcatcaa tggcaaaaac
      421 gacaaaatcc ttttgatcaa taaacttctt tgtatgagca gtcagctttt tttcaattac
      481 ttgtcctgct gtcccagtag tcagctttcc atcctctgca acaaaatcat atgtgtagtc
      541 aatcgagatc aatgcgttca tcttagtcct ccttatacga gatgtttgac aaattcgatc
      601 accatttctg ctgatcgttt gcctgcgtct tcgataaatt cgtcaaagct ttgtgtcgcc
      661 gagtgatctg ctgtgtcgct cattgcacgt acgatcaaaa atgggatatt gaactgacga
      721 gctgtttgtc caacagcggc tccttccatt tcgcaagcta atgcttcagg gaaattcgtt
      781 aagatttctt tgattttatc tggcgagtcg acaaatgtat cacctgtaac gatcaatcct
      841 tcttttgcgt tcaaattcgt tgcttttgct gctttaacca tttcactgcg caaatatgtg
      901 cttgcttcat aatatagagg cattcctggt aattgtcctg ggttgtagcc aaatcctgtt
      961 gcatctgcat caaaataagc tactttgtct gatataacga tgtcacctac ttgtaagcct
     1021 tcaccgattc cgccagctga acctgtattg atcaccatgt tcacgccgta ttgttggatc
     1081 aacagacttg tggtaataga tgctaagact tttcctatac cggaacgtac gacgatcact
     1141 tcgtggttac ctaatgaacc tgaaataaat aaagcgcctg cgcgttccca tgataatggt
     1201 tcgcttaggt tctctctcaa aatttttact tcttcttcca ttgcgccgat aataccgatt
     1261 ttcatctttt taattccttc tttctatata aatgcgattg ctaaaaaaac cacacataac
     1321 attaggataa caataataag agatttcatc aaaaaagagt tccatttggt cgtcttttcc
     1381 cgttcgctga tccttgtttt cgtgattggt ttattctttt tgctttcttt acgataaaag
     1441 ctggcaatct ttttttcttc ttgctgataa gctgtttctg cttttcgctg tttttttaat
     1501 gactcgcttg cttgtgcttg ttgtcgcttg cgaagttcgc ttcgcgtaat aagcggtcct
     1561 ttggccataa gccgatccct ctactttcct ttggtcaata gttcccagta ttgaatcgca
     1621 taaatcgtct ttgcatcaca aatcaactga tctttcatcg cttgttgcgc ctcttcaaga
     1681 gttagatgat acaactccag tacttcgtct tcatcttgtg ctaaaggatt ctcgaccttc
     1741 tctacccctt gtgcatgata aatatgaagg acttcatttg caaaacctgg tgaaagatac
     1801 atcgaagtaa gatggctcag cgacttcgca cgatagccag tttcttcttc taattctcta
     1861 gctgctgtca tttcgggatt ttgtccttct cctggatcga tttttccagc tggaatctcc
     1921 aaaatcactt tttctaaagg ttttcgaaat tgtttgacaa gaagcagacg atcttgttcg
     1981 tcaaaagcaa tgatcccgac accgccagga tgaaatacca gttctctctt cgaagttcct
     2041 ccatctggca aacgaacttc gtctacagca acatcaataa tttttccgct atagatttct
     2101 ttgcgactga ttgttttttc ttcaaattgc tcattgttca gcatatcttc tcctgctttc
     2161 tttgtaatca taactgcttt tttttcattt ttcaattttt ctcaaaaact ccttattagt
     2221 ttaccataga taaaccttag attttctcta aatatagaaa atccttcttt agtccgatac
     2281 tctttatagt agtccgtatg ctaaaataag tatgtaatcg aataacgaaa agaattcaat
     2341 actcaacgat aaatgacatg gagggtttat tatgataaaa cgaaattggc cgtggcttct
     2401 agcattggtc ttgctatttc tctttgtccg aaacaatagt ttttcttttg ttctaggact
     2461 tttattgata ggtgtagttt tagttttgtg gggactcttt ggttcgcgca ggaaaaaggg
     2521 aaaacaagaa gaaattcctg cactgactga cgaacttgaa actcactatg aaaaaagcgg
     2581 tatgacagcg aatgaaattt cattttttcg acaaacaatg aatcaaacaa aaaatgagat
     2641 caatcaattg caaatgaata tgcagcagac agccaaactt aaagcaatcg atctccgcca
     2701 tgatccagtc aaagcagcga aagctttatt caaagaattg gtgaaagaac caaaccgact
     2761 gcatgaagca agtcagtttc tttatacgca tctgccgaat ctagttgatt tgacaaataa
     2821 gtataatgag atcaatgacc atgagatcaa aaacaaacag acctatgaaa aactagaaga
     2881 aagcgcgcag atcatcgacc agctgtcagc tttaatcgca caagattatc aaaaattcgt
     2941 tgctgaggat ctagatgata tcgacgtaga gatatctgtt gcgaaacaga gcttaaagcg
     3001 tgataataaa tatgaaccag aacaaaagga ttaactcttt tgttcataaa agaagactcg
     3061 gaacattcag atgatgtctg gctgtttcga gtactttttc taaagtagag acattcatgt
     3121 aagaatcgag aacagattca acaaggagga attttttatg gatcataaac cgactgaaac
     3181 aaaagatgtt acaccagtta acgatacact tgaagattta ttgaacaacc cgttttcaac
     3241 tccaacagat acgctgacca cttcacaaca agcggagatt gatcagctcc aagagcaaca
     3301 gacagctgct cgtttaattg acaaactacc ggcggaacgc caagaacaag ctaaacagct
     3361 agcagcaaaa atcgatgcta atgatgcaca gtctgtcatc agctacggat cggctgccca
     3421 agcaaaatta agtgaattct cacaatcgat gttgaatcat gtgcaagcac aagatattgg
     3481 tcctgtagga gattccttaa ctgaactgat gtatcgtctc caagaagcca atcctgacga
     3541 actgcgtgcc ggagaaggga acatcttcca gcgtgtcttc gggaaggtca aacaatcgat
     3601 ctatgaagtg acagcaaaat atcaaaagat tggtgcacaa attgacaaga tttcagtgaa
     3661 attagataaa gaaaaagacg gtctgctaaa agataatctg atgttagaac agctttacca
     3721 gaaaaacaaa gactactttg atgcactgaa tatttatatc gctgctggag aattgaaaat
     3781 ggaagaactt caaacaacga tcattccaga agcgatgaaa agagcagaag aaactggcga
     3841 tcagatggat gttcagattg ccaatgatta cacgcaattc ctagatcgtc tggataaacg
     3901 tacgcatgat cttcgcttag ctagacagat cacgatccag caagcaccac aaatccgttt
     3961 gatccaaaac acaaatcagg ccttggctga aaagatccaa gcatctatcg ctacagccat
     4021 tccgctgtgg aaaaaccaag tcgtgatcgc attgaccttg cttcgtcaaa aagatgcagt
     4081 aactgctcaa cgccaagtct ctgaaacaac aaacgactta ttgaagaaaa attcagaaat
     4141 gctgaaaatc tcagccatcg aaacagcgaa agaaaatgaa cgtggaatcg tggatatcga
     4201 gacccttcaa accactcaaa atgatttgat cgaaacaatc caagagacat tgcgtatcca
     4261 aaaagaagga aaagaaaaac gtcgtcatgc agaagtagag cttggacaca tggaagaaga
     4321 tctgaaacaa aaactgttgg atttaaccca atagatcacc tttcgcttgt tactaaaaca
     4381 aacgcccaac aacagtttat tacaactgtt gatgggcgtt tttcttatct ttcttctaac
     4441 ttctccattg gagtctattt ctcattcatc ttgctgtgtc catagccata gccaaaataa
     4501 atgatcagtc caataaccat tacgataccg aacacgatcc atgtcacagt ttgcagacga
     4561 agcatcaata caatacaagt caagatggag acgattggca atagcggaac gaatggcact
     4621 ttgaattctc cagcttttgg tttgccaaac atttttctca acttcagtaa gcctagacta
     4681 acaagcatca aataggcgag cgccatgata ttcgttagtt ccgctaatac attcaatggg
     4741 aaaaatccgg caaagaatac agctgccaca ccagcggcat aagtcgcttt tttcggtgta
     4801 cgatgctttt gatcgatttc tgtcataaac ttaggaagta agccatcctt gctgacagca
     4861 aacagtaaac gagcaagtga atacatcatc gagatcgtta ccgtcagtag cgttaaaatt
     4921 gcccctacag cgatcaccgc gcccactgtt ccatgtccta catacctcat tgcaaaagcc
     4981 actgggtcat tgactcctag ttcagtatac ggaacgattc ctgtcaagat cagcgtcacg
     5041 atgatgtaaa ggacagtagc aatcagtatc gaaccaatga ttcctcttgg cacatctttt
     5101 tgcgggtttt tgacttcctc ggctgccatt gcaacggcat caaatcccaa gaaagcaaaa
     5161 aacacaagag ctgcaccatc tagcacaccg gaaaaaccaa aaggcatgaa tggctgccag
     5221 ttatcaggtt taacgaaaaa tattcctaca atgataaaca acgcgatgat accaaatttg
     5281 acatatacca tggcattatt caaacgtaat gctttcttgg cttcaagcga cacgatgtaa
     5341 gtgacgaata tcactacgag caccgcaatc aagtcaatgt atgtaccatt ttccggattg
     5401 tacccgccag ttaatgcttg tggaagttcg gcccctaaac ttttcaaaaa accttgcata
     5461 tagccagacc atccggaagc aacagaggaa accgctagta aaaattcccc gatcaacaac
     5521 caccctgtca gccaggcagc aaattcacca aatacaacat acagataggc atatggccca
     5581 ccaatcactg gcactcgcga agcaaattct gcgaaactta gtccagacaa aatgacaacg
     5641 attgcagcta agacaaatga aagtgacagt gctggaccag cattttgatt ggccgcaacc
     5701 cctgtcacca caaatattcc cgtaccgatg atcgcaccaa ttccaagcat gatcaaatca
     5761 gacgttttta gttctttttt catctcactc gtatgattga gattgatctc tttcttacga
     5821 aacaaatcca ttgaagttcc tccccatttt aggttggaaa acagcgatta aactgttttc
     5881 atatacccta aaaggatacc acctaaaatg actaaaaggc aaccaacaat aacataaatg
     5941 agctcttttt tcgtttttct ttcgccaagg atgaaaatcc cacctaaagt agagataatg
     6001 atccccattt gtgaaagtga gaagccaaca gctaaaccaa gtgatttgac tgttagaagc
     6061 atgcagatgt ttcctaagcc ccataataat ccacagatca tgtttttcca cacaaactga
     6121 tcgactttaa ctttacggaa agcaaagaag cttgcgccta agatcattcc gatactttga
     6181 ggtaagataa ttgacatcgc atccaaattc gcccagttga cgatgatggt atatacacca
     6241 taaccaatcg tagagaagat caatgctcta aaacccttac caaaatcaag cattttatta
     6301 tctgtatcta tttttccttc gtcatcttgt cgggcagtca aatacgcacc gcttacaaga
     6361 agagctaaag caataatacc gtatacataa tctttcgttt gtgtccattc atggaagaaa
     6421 acggctcccg caatcgtatt caaaattaat tgaaaaccag tagacaatgg tagccctaca
     6481 gaaacaccca tgtacttcat tccatggaac tgtccatttt gtccaacact ccaagctaag
     6541 ccggataaaa atccgatcaa gaaaatccaa ggggtgatta caggactaac aacgaaaaag
     6601 acaactaaag aaaacagaaa tgcaccgatc gtcattccta gtgtttgttg attggcatcg
     6661 ccaccaattt taccacttac taaaccaata ctaccccatg ccaacatagg gactaatgct
     6721 attaaaattt ccataaccaa tctcctttta tttcacatga taagaaactt atcagcgaaa
     6781 acgttaacaa cacaagcact ttttttcgtt tctaacgatt ttctaacgtt tttcattata
     6841 aaaatttaag attgggaaaa ataaagtcat tgttgtccct tttccttcag ttgattcagc
     6901 cgtcaaatgg atacctaatt tttcggccag attttttgct aaataaagtc ctaatcctgt
     6961 tgaatgtgtt ttgctaagtc gaccatttct cccagtaaat cccttatcaa agatccgttt
     7021 caagtcttcc gctgggatac cgatccctga atctttaact gaaaggctga ctccttgcgc
     7081 ttccttttct atgctcacaa tgatgtttcc gtgatccggc gtatatttga tggcattact
     7141 aagcaattgg ttgaagataa aagcgatcca tttagcatcg gtcaaaacag tatgatcctc
     7201 accaacgatc tcgtagcgaa tatttttttg gataaaataa tttgcctgac tgcgtatcac
     7261 aggctgaatg attcctttta gtccatattc ttggatgagg tagtcccgcg agaaactgtc
     7321 gagtcttgca taatacaaaa cttgttctac gtattcatcg atttttgata attcattttc
     7381 tagtaatgta tacttttgat cgtcaatatc gaactcgatc gaacgaacta gcaatttaga
     7441 agcggaaagt ggcactttga tttcatggac ccatgagtca atataatctt tttggtcttg
     7501 ttgattactg attgctgatt gcattacttc ttggtgttca cggatcagct gattgatata
     7561 ttcttctacc agtttttctt cttctgtcct cccaccactc aaataatttt gcaaagggga
     7621 agttttttgt atgtcggcta attttcccca ccatcttctc ttgctcaagt aaccacccag
     7681 cagataaaaa atcagaaaga cgccctcaat caaagctaaa taagcaattg tgctcaaatc
     7741 aactttgatg tttggcgcca gccacatcac aaagatcgtc aatgcaataa aaaacagcca
     7801 tcccaagatc aatgtccact gatctttcaa atatttaaaa aaattcatct taaccgctca
     7861 tcctttcagg gaacgatata tccctgtcct acttttgttt cgatatatcc ttggatacct
     7921 gcctgttcga tttttcggcg taaccgattg atgttgactg tcaatgtatt gtcatctaca
     7981 aatcgttcgt catcccacag tgctcgcagc aatttttccc gacttaagat ctttccattg
     8041 tgcttcatca aaataaataa tagacgatat tcatttttgc tcaaatcaat gatctctccg
     8101 ttgatttcca tacttccgtt gtcaacattc aacgtaatgc cattgtggct catcatttca
     8161 ctagataatt ctgtgtagtt gtaagaacgg cgaagtaatg cattgatttt tgcaatcagt
     8221 acatcgattt ggaaatgttt tgtcacaaaa tcatctgccc ccatgttcat agccatgatc
     8281 atatccatat tcgtattcct gctggagata aaaatgatcg gtactttcga tacttcacgt
     8341 attttttgac accaataata gccatccaaa acgggaagat taatatctag taatacaagc
     8401 tgtggttctt ctctttcaaa gtctgagaac acttgattga aatcggtcgt tccaaaggtt
     8461 tcaaattgcc atttctcaag ttcctccatg atcaattgtc gaatgatctc ttcgtcttcg
     8521 actaccatga tttttgccat aaaaaatccc tccatctttc ttcttgctac tacaatatca
     8581 aagaagccct tgtgaaacaa gttggcttac ctggaaaatg tcattttagc aaacgttaaa
     8641 aaaaactgga aatatgcgat ttaatcgcgt ttgtctcttc caagcagctt aaatttatta
     8701 tgttattctt aataattggt ctattatctg ttatttttct ttcgttgttt ttgtctttta
     8761 aattttcgat aacaccaata accgaaaagc gttagcacta gcaatatacc tagaaggagt
     8821 ataagtaatc gataaagatg gtattttttt gtctgttcta tctttgaaga gacttcttct
     8881 gtaaagggaa ctcgtcttcc tgtcactagt aatcggtgag tgttcacggt gtaaggtgta
     8941 caagtgataa gtgtgactag atcctgcttt tctctaattg ctaatgtatt tgtttttgtt
     9001 ggaaggacaa caattttctc aacgacttca taagcgagca tccgaccata cacttcaaga
     9061 tagaatcggt catcttgctc catttcttca agatccgtaa agagtttttt ctcagcaata
     9121 ccgctatggg cggtaatcac ggaatgtgta ctctctcctc cagttggaaa agaggtgcct
     9181 tgcaggactg tcgctcctct atctaacaaa agatcattcg tttcatcaaa taaaggcaag
     9241 ctaacattga ttttcggaat atagatggct ccgatcatat gttgttcgta atattctttt
     9301 cctggatttt ggacatttct aaccgcttgc tcaaaaggat ctttgacttg tcccattcct
     9361 gggattactg atgtatgagc aagcgtctga tttttctttt catgcactgc taattgttgc
     9421 tcatgttttt cttctttatc gcgttcaagc tgcttttgat aattctcgat ccttctttga
     9481 tccaaataat tgctgagagc atctgctaaa aatggatatg taaaaactgc agctccggca
     9541 agaaacatca ctgccataaa taattgtaat aaaagactga tttgggtttg aggtctatta
     9601 cctattttct ttttcatttt cttcaccttc ttatttaaaa aaactgcagc aacctttata
     9661 aaaccgattg ctgcagctta tttttgttgc ttatacttca gacttcattt tagatttacg
     9721 gtaccagctg tacgctccaa tcatcaaact tattccgatt agtaagaatg ccaagagccc
     9781 gttcccacca gtagcaggaa gtgacccgcg tttcatattc ggtactcttt ctggccctgg
     9841 attttctact cctacgatct gaatttcttc attatagctt ccataagaga tgataaattc
     9901 agtaaaggct gcctcacctg ttggaagtac ataaccttct ggcgccatgg tctctttcat
     9961 ttgataggtt ccataatcta acccttgaac ctcaaagtat ccatttccat ctgcgacaaa
    10021 ttctgttgct tgtccgcttg ttgcccactc aattctatct gctcctgatt cataaacagc
    10081 taaaggcgac tcccctttat aaaatattgc gtattcagaa cggtcttcat tccacaattc
    10141 gaatcttgca tctttaagca attcactgct tgatgcatca tatttttcaa actttcttcc
    10201 acctgttgtg actttctctg gcggctctac tccttcttca taatcaaatt catcatttcg
    10261 tccgatttca ataaccgctg tgttgttatg gaaatcatcc gcttctagtt ctgtggaaac
    10321 cttcaaatta tacgtgatcg ttaaatctcc tccacgtgca tctgctaatc tacctaaagt
    10381 atctactttg gctactccat tttcttctgt caaaatcgtc acgacaaacc cgttgcttac
    10441 tacttctact atataatctt gtccttttgt taaaatcgtc tcgccggctc gcacttctat
    10501 ttcctgatca taataagtta gaccaactgt aggcgtatct gtgattttgt aatttttgat
    10561 aacagcgctt ccgttttcaa ggacagaatc gatgaaataa ggaataggtg ctgtgatcgt
    10621 gtaagaagcc gtttttcctc tttctagatc aatataagaa atcgtttctt gttctccagc
    10681 ttcattaata atcgttactt gtctaaggtc gctttcctct acattcatct gtttatctgc
    10741 ttctctgatt tcgtttttcg gatacaaatg cacatctgta ttgtatgtgt ctttccatgt
    10801 agaaccatcc cacacgccat ccaccatatc aggcatcatc actggcatca taacaaccat
    10861 tggagacgcg attctggaaa tatgagctgg cgaataagtt tctaagaaaa gatagacttt
    10921 atctctagaa gaaggtgctt cttccgtcac ttggacggta gagaataccg cttctccagc
    10981 ttcattcgtt gtttgtttgt caatacttgt cagtcgatat tttaaaaacc aactttctgt
    11041 gtgccgtttt tgaatccatt caatcgcttc ggcagaatta agaccgtctt ctcgttccgg
    11101 tgttaaagga ttatctcgaa tcaaatcgta atatacttcc gttacgtcaa acaaatcaaa
    11161 atcgactcct ggaatatttt cttcaccaaa atccgtagaa accaatccgc tattcggtcg
    11221 ttcttccgga acagatgaga aacctttttt gtgtaatgta atcgtcactt cacttggttt
    11281 ttgtgcgact tcttcggctt tgactagttt tccgttcccg aaaatcccta ttcccgcagt
    11341 aacgagtaac agtacactca ttcccgtgac aatcattttt ttattcattt ttttctcccc
    11401 gttctttttt tattaataaa acagcaccta acaataaaag aattcctagt aaagaaatca
    11461 acgttcttgc ttcattcgtt tgcgggaata cggaccattt tcctgattga cctggattgc
    11521 ttggaccact cggctctttc ggtttgtccg ttgtttcttt tacggtctcg ttataaacac
    11581 gtggtgttgc ggattcatag gcagagactg gaactttccc ggattctaat tcagccatct
    11641 cctgtccgtt aactactaca tattgtggtt ggccttttgc atccacccaa aattcaggga
    11701 tgatgacctc gattctctta cttgcttcat ccatctcgta accttccaca ccttttaatt
    11761 cttcaaaata ataggtgcca gaaggcaaga aacgttgtcc tgtcgtaaca aggccctctt
    11821 tatccgaaat gaatttgctt acatttttgt cattcaaagg atcattgtct gctggtttga
    11881 tccattgatt tttcaagtca ttagcaggag ccatatctaa ataatatttc cccccttcta
    11941 tcatctgata tagagcaaaa acagcacctt ctaatggttt tcctttttca gttgttcctc
    12001 tttgacgtcc atacttgaag aagtatggat ctcttagata accgacattt ttaggataga
    12061 tatgaatctc ttgcagttcc tcggttgatt ctgtcgggtg ataaattggc aaaatagcag
    12121 caattgggat agctgtctga tcgatatcaa catccaatcc aacttctgga tcaatcgatt
    12181 cttcgaaaat caaataaact gcatcccgat cttggttttt cttaggtgct ttgatacgtg
    12241 caatcccgtc tcctaatccg ccaaccgtat ctttcccagt tgttaccgaa gtccctattt
    12301 ggacaagcct ttcttctgtg attagctgac ggatattgtt tctgttcgtt tcagcgattt
    12361 gctggacgaa ttctttagga gtcatgtttt cagcagcttt tttttcatga taataatcgg
    12421 tcatgtcgta aatggcaaat gtcgctccat ttagtggcac cgtctgtgaa atgatatctg
    12481 ctgtttcccc tgcttcctca gagatagcca agccatcatt ttcatggtat tcaaattgat
    12541 cgttataatc gatatctcga atcatccgtt tatgcaacac gaattccacg acatcttccc
    12601 ctgattcttc tgccgacatc gcatgtggaa gaaggactgc tgcaagcggt agtaaaacaa
    12661 gtaattgaag catcaaacac aacagctttt tcattacctt gctccctcct ttttagattg
    12721 tctgacaaac aaaccatagg ctgctcctgc aagcgccatg aagaatgcag caagcaggat
    12781 atagaataat cgtccggatc ctcctgtagc aggcaacgga ttttcaggat cgttcccgac
    12841 actaagctgt aatctgattc gatttgtctc caaaagttca tattctaact cttgctgtag
    12901 atcaggcgtc tctgcttctt cgtttggata atacgaaatc tcaactttta gtgtacccgc
    12961 ttcttttcct ggaactaccc gatcattttc tatgtcaggt tcttctcgat aactaatttc
    13021 taaaataaag taacctggaa gttttctata gctgtcaggt gacttcactt ccttgatccg
    13081 atagacccct tccaacaagt ctgaagcatt gaatagggaa gaatcgtttg gatctggtgc
    13141 caagcctccc tcgatgattt catactctcc tgcactattt tgtttctcaa ttgaaaattc
    13201 tgcgccttca agacgtcggc tattcatact gtcaattttc aaaagttcca aatcgattgg
    13261 cgctagttca tcttctaagg ttacttgtag tacaccatcg atgatttcat agccttccgg
    13321 caagggagca gtttcgtctt cgatgaccgt atcttcaatg ataaacttac cttcttcatt
    13381 cagtgcaaac tggaacactt gatcagatag ccgataccca tttggtgcga cagattccga
    13441 gacctcatat tcaccaatag acagatgcag aggttggtcg taatttaaat cgacatactt
    13501 ttctactcct acggcacgag aatagaagta tccgatccca ggcaaacgtt tcgattcttc
    13561 tggttctaac ggattaccag tgtatgctcg atacagcggg aaggaagaat cccccacttt
    13621 cctgatatcg aaacctgcac catctaatgt atggccttca tggtctaatt ttctaactcg
    13681 gatctcccga taatcaaacg tattgtaagc agtgaatgct acccataaac gatcctcttc
    13741 atctgttgta tacgtgagat attgccaatt ttcttcacct gtttgcttga gttggatagt
    13801 ccctatagca ttatcgtaaa gtcgcaagat ccatgtgtca ttttcatcag gttctttgaa
    13861 accttctggg ttctggctct caatgatttt gtattctgca tagttggtag tttcttcact
    13921 atccttataa acaaatgaat ctggctctcc gattggatga ataactttct caccatccac
    13981 gattacatat ttcccgtctt catctactga atagaacgtg agtctccctt tctcttcctc
    14041 tacttcgttc acaccagaaa gccctttcgc tactggatat ccgatttcca gtccgttacc
    14101 atctgtttca taaagttcaa actctgcgcc attgattggc atattactgc cttcaattag
    14161 tttattgata ttcatgtgga ttggcttgaa ttcatttcgc atttccagtt tgacatacaa
    14221 ctcatcatac tctgtgcctt ctatgttagg ctcttgtaca acagaaagct tgtgatattc
    14281 tccttcataa taggaattcg gatctttacc ttccggtccg ccgactctcg aaccagatgt
    14341 atattcatct acaagctcgt agtgacctaa catctcatct tcgttggctg catatttttt
    14401 gatttggaat tgatacgtct catctactaa ctgataacgt ggtggtccag cgatctctct
    14461 gatccaaaat atgtcgctga ctgctccagt ttcatctcct aaaatcggca tcttcgtttc
    14521 aaagatcgct attccgtccg tggtagtttt acttgtttcg attggttcgg cttccccact
    14581 ctctggattt tggtcatata atgcaaattc gactccagcc aacggctcgc cattttgacc
    14641 cttcttgctg acttggaaat tacggccttt atgttcgtaa cgaatggaag aagatgggaa
    14701 atacatccgt ccagtagtga tatcattctc ataatcctcc aggttctgaa tataggccat
    14761 attgttcact gtgcaaaaat ttccgtcttg agctcccgca tcaagtttta cttggtactt
    14821 gatcctgacc atttcccctt cacctaaaga aatcggagaa aatttataag cttggttttc
    14881 ccgtccgaca tacgttgtcg gagtcgccac tacttctggt ggggcttgtc catgacgttc
    14941 ttcatcccat atttctggct cttggtactt catgccgttt tctcctggat ctagatggta
    15001 aaactgaata tctgccgatc tgccagctcc aaatggtgag acattaaaac cagtttcatc
    15061 ataaagtgtg gcttgctgga tcgttctctt gaaggtagaa gagatatcct ccaaggctgt
    15121 aacaatgtca ttcttgttgt tcgcattgta atatcgcggt gttacgccat cttcttctaa
    15181 atcagcaata tttttcaata catttcgtcc aatagcatct agtcgttgtc tcccgctaac
    15241 gtttccatcg attcctaaac caatggagaa gatacttggc gggttctctt gatacttgtt
    15301 tttcagccaa tgatgataag caactgtgtt gatcgcagat gacttttctt gcgaagtagg
    15361 actgccattc gtattgttat tagaagaacc atcaccaacc cagtgagtcg ctttattatc
    15421 tccttctttt acctctccat aacgatagtg tacatttact tcatcttctg gacggttaaa
    15481 gtcagatgca taggtaactg gatgagtaaa ccctgcatta gaagtattcc cagaaaactc
    15541 ctcaaaattt ctgaacaaaa cgtcattatc ttcagcaatc ttatcttcca tcacagtcca
    15601 gttgctccaa gctccattag tgctcgtacg ataacttgac tgaatcggtg cataactgaa
    15661 ggtcggtgtc ccatctccca caacgattag gattttttct ggatttctat tttctccgcc
    15721 attgtcttta tacaatgttt catagccatt tttaagccct agtgtaagcg gagttcctcc
    15781 gattggtgta cgatttaatg ctgtattctc acttcccgtc aaacgattga tatcatttgt
    15841 caatggaaag atgtccgaac tgatttgctc atggttattg atagctgtac tattatggta
    15901 aaaattcacc ataccgattc gaatatccag actcgggtca gagagcagat ctttactcat
    15961 ttcgtttact gcctcaatca acgcagcatt tttagtttga ctattggtcc cagcggttaa
    16021 ttcactcata ctcgctgact tatctagaac aaaaacgata tcgattggac tgatttcctt
    16081 tttctcgccc ccgatgacat ctagatagac attgtaaagg gttgttgcat cttcaggatg
    16141 tagatcagat gctgtgctgc ttggcagaac aggttctacc cattttttga tgtacgcccc
    16201 gttataatcc aagtaactgt tttcaaaatt ccgttcattt ccccaaagct gaacggtttg
    16261 agctggaacg tttctatctt cttcgcctgc agttgatcca ttattagtag catagtcgta
    16321 atttcgccag ttcgctcccc cgtttgtata atcactatca tcgatcaagt tctctccgtc
    16381 atatggcgag cctttgtctc cttgatagcg ttcattgctt tgtgtccaat ttcctaacac
    16441 ttttgtatag tcattcgttg taaacttcat ctattctttc atcaaccacc acttgaagag
    16501 gtagttaccc ttcgttcaca tcggggatct tttctgtttc aaaaatcaat gtgccggttt
    16561 ctgcttcttt tgaataaact tttccgacat accattcttg accatcgatc gtctcgactt
    16621 ttaggtcttc tccatcttca tcataggagg atagacctgt tccacgaacg ttttttacgg
    16681 taccaatccc ggcggctgcc gcatcgattg ctaaacgtat tctaccttca ttggcacttt
    16741 cctttttttc aaaagccaga tgccattcaa gggtttcttc tttttcaata tacttccctt
    16801 caatttttgc atagtcctca tctaagaata cctcatcctc ctttgtttct gttgactcag
    16861 cagctacatc cagggtcgtc atatgataga ctgactggaa atatggtcca aatagcaaac
    16921 tgacagttac taactgcaaa agcttcaatt ttaacccttt cacatcctta tactcctttc
    16981 aaaaatattt gtcttctttc acaacaatca acaaaacaat gccc
//
LOCUS       DAZUMK010000050        16704 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000050 DAZUMK010000000
VERSION     DAZUMK010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.051"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(78..1583)
                     /locus_tag="ACG808_001575"
     CDS             complement(78..1583)
                     /locus_tag="ACG808_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5047203.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1580..2266)
                     /locus_tag="ACG808_001576"
     CDS             complement(1580..2266)
                     /locus_tag="ACG808_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5047204.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2459..3880)
                     /gene="gndA"
                     /locus_tag="ACG808_001577"
     CDS             complement(2459..3880)
                     /gene="gndA"
                     /locus_tag="ACG808_001577"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFS5047205.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            complement(4050..4229)
                     /gene="rpmF"
                     /locus_tag="ACG808_001578"
     CDS             complement(4050..4229)
                     /gene="rpmF"
                     /locus_tag="ACG808_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFS5047206.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(4287..4856)
                     /locus_tag="ACG808_001579"
     CDS             complement(4287..4856)
                     /locus_tag="ACG808_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFS5047207.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            5042..5944
                     /locus_tag="ACG808_001580"
     CDS             5042..5944
                     /locus_tag="ACG808_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFS5047208.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(5979..7763)
                     /gene="pyk"
                     /locus_tag="ACG808_001581"
     CDS             complement(5979..7763)
                     /gene="pyk"
                     /locus_tag="ACG808_001581"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFS5047209.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(7837..8799)
                     /gene="pfkA"
                     /locus_tag="ACG808_001582"
     CDS             complement(7837..8799)
                     /gene="pfkA"
                     /locus_tag="ACG808_001582"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFS5047210.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(8967..12281)
                     /gene="dnaE"
                     /locus_tag="ACG808_001583"
     CDS             complement(8967..12281)
                     /gene="dnaE"
                     /locus_tag="ACG808_001583"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFS5047211.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KRQLIYLLGEDNVVFK"
     gene            12512..12697
                     /locus_tag="ACG808_001584"
     CDS             12512..12697
                     /locus_tag="ACG808_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFS5047212.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(12759..13955)
                     /locus_tag="ACG808_001585"
     CDS             complement(12759..13955)
                     /locus_tag="ACG808_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFS5047213.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(14125..15561)
                     /locus_tag="ACG808_001586"
     CDS             complement(14125..15561)
                     /locus_tag="ACG808_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFS5047214.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            15854..16663
                     /locus_tag="ACG808_001587"
     CDS             15854..16663
                     /locus_tag="ACG808_001587"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5047215.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         4893 a         3518 c         2934 g         5359 t
ORIGIN      
        1 ccctgaattt ctcacttgct tgtattttgt accttattct ttgttggata tataaaaaaa
       61 caagtaaagc acatgcttta cttgtcttct gcctttgcga taggaataaa gattctaaaa
      121 attgtccctt gccctactac actttctgct aagatacgtc ctttatagtt ttcaaccagt
      181 tgtttagcga tagacaaccc cagaccattt ccacccttcg ttctagctct tgctttatcc
      241 actcgataaa aacgattgaa gatcttatta aggtcttctt tgggtattcc ttcgccaaag
      301 tcctgaatcg cgatctcaaa ttcattcaga gtggaagaaa tagagatatg gacttctttt
      361 cgtgtagttg aatactttac tgcgttatcc aatatgataa tgattaattg ttcaaagtga
      421 ttacgataaa tttgcagggt cacttctttt gtcaaatcat catctaaagt gatcacatag
      481 tctggatata atatcttgaa attattataa acttgatagg tgacttcttt tcctttagtt
      541 gtttcattcg cataatgaac atctacttgt tctgctcttg ataagtctag catctcttga
      601 actaagcttt tcatacggct gatttcttgc atgctggcgc ttaatgattc ttctaatatc
      661 tcaggatcat ctttccccca acgatttagc agactaagat gcccttcaat aattgcaaca
      721 ggtgtcctta actcatgaga tacgtcttcc acgaattgct cttgttgttc aatatacaaa
      781 cgcatacggt caagcatctc attgaatatt tcagctaaat ctgccaattc atcatttgtg
      841 tcgatttcag gcatgtggat atctgattgc ggatctttac gtatagtatc cattgtatct
      901 ctgagtacct tgagtggttt taagaaataa gaagataaaa agaaacctaa aatactactt
      961 aagatcaaag aaacaacttc taaaacaata agagtcaata atagcttatt tcgaatatca
     1021 tagaaagaag ataattcata gaagctttgc gcatagccga tcttttctct tgtaccttta
     1081 gaatatatag gctggataga taaaaagcca gtttttcctt ctagtgtaac gatcgttgga
     1141 actttacgtg tagtctgaat caggtttcga ctgttttcat gggttttaaa aatcaagttc
     1201 tcgtccaaat cataaacata aagatccatt gatggctgcc ctagctcagc tataaaggaa
     1261 tcgatcttca ttaagtttcc ttcgaccagt gaagtatgat ctacgacctc atctccgtca
     1321 tttgtcgctt tcgtcagatt acgatacgta tttactaatg tcagttcttc atccgagtta
     1381 gccaaacgag aagctacttc tgaaatcgta cgctctacat tgtttctttc ttttgccaca
     1441 atcagattga tagatgattt ataggtaatg acagcgaaaa ttgtaaacac aacgaatatg
     1501 aagaaagaac tcgcaaaggc ccacttgatt gtcaaggagg gccctttcag ttctcttttt
     1561 tccgattttt tcggctttat cacgagcgca tcacataacc tgttccgcgt acagtttgga
     1621 tatagctttc ctccccggga acgtcaattt tgttgcgaag ataacgaata tatacgtcca
     1681 ctacgtttgt ttcaacttct gtttcgtatc cccacacttt atttagtaaa acgtcacgtg
     1741 ctaataccac gttcacgttt tccattaaag taagcagcaa ctcgtattct ctttttgtta
     1801 gttcaattac ttcatttccg cgacgtacga cacggttttc tttttcgata gtcaaatcgc
     1861 gataagtaat agtagtttgt ttagccacat ttttatcgcc ttcaatatcg attcgacgca
     1921 acaatgcacg aagacgtgct aataattctt cgatagcaaa tggtttgacg atgtagtcat
     1981 ctgctccgtg gtcaagtcca gatacacgat cgatcacaga atctcttgca gtcatcataa
     2041 tgattggtgt atttttgact tggcgtacac gtctgcagac ttctaaaccg ttcaattctg
     2101 ggagcatcag atccaacaat atggcatccc actcattatt taatgccgca tctaaaccag
     2161 ttcggccgtt gtagtggact tctgcgttat acccttcatg cttcaactct agctcgacaa
     2221 atcgtgccaa gttcttttca tcttcaatga ttagaatatt gctcatttat tattttatcc
     2281 tttctattct actggctttt taaaaactct atcattatac cgataatatg cttaaaaatc
     2341 tatagtcatt ttattatgga tagcaatttt aaaaaagagc tgaagtctac tcataacaat
     2401 cgacttcagc tctttcgtcc tctttttatt ttctcatttc ctaacagccg aaagactatt
     2461 attcttcatg gtaccatgag taatggaaga ttccttcttt gtcgacacgc tcataggtat
     2521 gtgcgccaaa gtaatcgcgt tgggcttgga ttatatttgc aggcagacgt tctgctctgt
     2581 atgaatcgaa gtaagcgatg gctgatgaga aagtcggtac aggtacgcca gcttggacag
     2641 ccaaggcaac gacgtctctg actgattgct gatatttttt cgtgatttca acgaagtaat
     2701 catctagcaa taaattttcg atctcaggat ttttgtcata ggcatccgta attttttgca
     2761 agaatcgagc tcggatgata catccagcgc gccagatttt tgcgatttca ccaaatggca
     2821 agtcccaatc aaattctttt gaagcgacac gtaattgagc aaatccttgg gcataactca
     2881 tgattttact gaaatacaac gcttctcgga ttttttcgac caattctttt ttgtctcccg
     2941 tgaactcaaa atcgttcgta cgagacaaaa tcttgcttgc ttgtacgcgt tcttctttat
     3001 aagcagaaat atagcgggca aatacagatt cagtgatcaa tggaagtggc acacctaagt
     3061 caagcgcgct ttggcttgtc catttacctg ttcctttatt cccagctgca tcaaggatca
     3121 catctactac tggtttacct gttccttcat cgtctttacg tgtcagaatg tcagcagtga
     3181 tttctatcaa atagctgtct aattcgcctt gattccattc tttaaagata tcagccattt
     3241 cttctactga aaggtttaaa atattcttca ttaagtcata agattcagca atcagttgca
     3301 tatctccata ctcaataccg ttgtgtacca ttttcacata atgtccggct ccatttgggc
     3361 caatatatgt gacacatggt gctccgtctt ctgcttttgc agaaatttgc tctaagattg
     3421 gtgcaactag cgcataggct tctttttgtc caccaggcat gattgaaggt ccttttaaag
     3481 ctccttcttc accaccggaa acgcctgtac cgataaagtt gatgccagag ttggctaact
     3541 cttcattacg acgcatcgta tctttaaaga atgtatttcc tccgtcaatc aaaatatcgc
     3601 ctttatcaag gtgaggaagt aactcttgga ttgttgcatc tgttgccggt cctgctttga
     3661 ccattagcaa gatccgacgt ggtttctcga tagattctac aaattcttcg attgtatatg
     3721 ttgctttgaa atttttatct gggtgttctt ctactacagc ggttgtttta gcccctgtac
     3781 gattgaataa agccactgaa tagccgcggc tttctatgtt caaggctaga tttttaccca
     3841 taacggccat accgacaacg ccgatttgtt gttttgacat atgaactcct cctacatacg
     3901 ggatttctcc ctaatctctt acattatagc ttaacacggc tacattgcaa actaaagaac
     3961 aacatatttt ttatttaccg actacaaaaa aataaacctc aaggctccat ggttagcctt
     4021 gaggttttac attttctaat taaaatgtat taagcttctt ttgacattac gtccttacca
     4081 tcatagtaac cacaatttgc acatacgtga tggctttttt tcatttcacc acagtttgga
     4141 cattcgttta accctttgat tgtcaatttg tagtgtgtac gacgttttgc ttttttagct
     4201 tttgatgttc ttctagctgg tactgccatt ttgctacacc tccttgtaaa gtcttgtatc
     4261 taaattagac acaatatatt ccaatcttac ttgttatcgt cgtcttttga tttagaatca
     4321 aaaagttcag atagttttgc aagacgagga tcaattttat tctcggcttc ttccattttt
     4381 ctgcgctggt attcttcttc ggaaacaact tcccaatcat tgccttttgg aagttctgtt
     4441 gctgcttcct cttcttttgt caatacccgc attggaatcg ccaataagat gttgtcttca
     4501 atcgactctg ctaaattcaa taatggacct tcaattagca aaatctcatc tgcagacatt
     4561 tgttcatctc tactttggaa ttgttcttct gtcatgaata cttcgtctac agaaaaatcc
     4621 atgggcagtt caacaggttc tagtgaacgt gttgaaggaa ctgtcaaagt cgtctgcaca
     4681 gtgtaatgaa cgatatagcc tttcttatca actgagacca aaccttcgac ttccactggc
     4741 gcaacatcaa gtattaaatt gtctcgcttc atgagagcgg ctttcaagtc gagcgtctca
     4801 tgaaaagtta acggtgtctc ctggtatttt ctcaattcca ataaagacca tttcattttt
     4861 catcactcct aagcaacaaa agcaattata cagggcaaaa agcatgttgt caatgaaatt
     4921 ttcttgacac cttgcagttc ataaaaaaac agaaaaatct ttattagaaa gcctctgttt
     4981 gctatcatcc tcagaatcat ttacaataat cattattcca ttaaaggagc ggatgatctt
     5041 catgaacacg aaagatatcg aatactatgt ccaaatcgtc aaacaaaaaa attttacgaa
     5101 agtggctaat tatttccgtg tgagccaacc tactattaca tatgcattac aacgtcttga
     5161 aaaagagttt gacaccgtac ttattacaag agatcgatcg catcacgaat tgatcgtcac
     5221 gccttctgga aagcagctgt ttctccatgc acaaaccatt ctccaagaga tttctactgc
     5281 aagagctgaa atcactcagt tgcaaaagaa aaagcttcgt tttggcatgc ctcctattat
     5341 cggcaatgaa tactttccta aactgtcaag ttactttatc aagcatgaat tgatgaatca
     5401 aattgagatt accgatggag gctctcggga cttatacggg atgatccgac aagggaggat
     5461 cgatttagct gtattaggct caacacagcc aatcgtggat caatatttag aaacagagtt
     5521 acttttggac aaacgtttca tgatcgtcgt gccacctaca catcctcttt ccaagcagga
     5581 tactgtctct ttcaaagata tagcagatga acagtttgta ctattgaacg aacattatgt
     5641 ccaccccgca gcattcacta agctttctaa acaaacacat actcatccaa aagttgttta
     5701 tcaaagcaat gacttgacga ttttaaagag tatggtccga gaaaatgtgg gtatcggttt
     5761 tttggcagag attgctgtcc atcctgaaga tcatttagtt tgcctccctc tttcagatga
     5821 agttcagcct cgcttcttga tttcattggc aaaacgaaca cagcagatcg tcacgcctct
     5881 tcaagaacaa gtagtccaag ttatccatga attcgttaca gcacaaaaaa acagctcttc
     5941 gtaaaagaag agctgttttt attttttatt aagctaaatt agatagctgt tgtttcgcca
     6001 cggtaaacga tgccgcgacg tgaatcaacg gtaatcaatt caccttctgt aattgttgtt
     6061 gttgcgcctt tagcaccaac gattacagga atatcttgtg caattcctac aactgcagcg
     6121 tgagaagtca atccgccttc ttcaacgata acagcagctg ctttttcgat tgcaggcata
     6181 tattctttgt cagttgatgg tacaactaga accattccgt cttttgcttt ttttacagct
     6241 tcttcagcag atgaagcaat aactgcattt gcaacgacag atttttgtcc aacgccttga
     6301 gcttctaata atttagaacc aatcatttga attttcatca cgttagttgt tccgcgttca
     6361 ccaactggta caccagcagt gatcaagatt aaatcgcctt ctgatgcgaa acctaattca
     6421 acagcttttt tagctgctag ttcaaacatt tcatcagttg ttgatggttt ttcagcaact
     6481 gttgggtaaa cgccccagtt taacatcaaa ccacgttttg tgcgttcgtc aaaagtaaca
     6541 gccaagatat ctgcatctgg acgatatttt gaaatcattt ttgcagtgta tcctgattca
     6601 gttgcagcaa cgattgtttt cacgcctaga ttttttgcag cacgagctac agataaaccg
     6661 attgtttcag ttacatctgt tttatcgaat tcgttcaatt ggaatgtacc taattctgat
     6721 aatgcatttt cagcttcttg gtcgatacgt gccattgttg aaacagcttc aactgggtag
     6781 tccccgtttg cagattcacc agatagcatt gttgcatctg tgccatcaaa tacagcgtta
     6841 gcaacgtcag aagcttccgc acgtgttgga cgtgggtttt cttgcattga ttctaacatt
     6901 tgtgtagcag tgataactgg gataccagca gcattacatt ttttgataat gcgtttttga
     6961 accattggta ctagttcagc tgggatttca acacccatat ctccgcgggc aatcataaca
     7021 ccgtcagaaa ctttgatgat ttcgtcaatg ttgtcgatac cttcttgaga ttcgatttta
     7081 gggaagattt gaacatgagt catgtctttt tcttctaaga tttcgcggat atctagaacg
     7141 tcttgtgctt tacgtacaaa acttgcagcg ataaagtcga tatcgttgtc taaaccaaaa
     7201 cgaatatcgt cagcatcttt tggagtaata cctggcaaac taatagaaac gcctggtgca
     7261 tttacccctt tacgtgatcc tagcatacct gcatttttaa cttctacgac taattcacgg
     7321 tttgcttcgt ctttttcaat gatttgcata tcgatcaaac catcatcaaa taaaatgtgt
     7381 ccgccaacgt gaacatcgtc aaataatcct gggtaagtaa ccgcgatttt ttcttttgaa
     7441 cctacatgtt caggatccat agagatgcgt gtttgatctc ctacttcaaa tttgatgtat
     7501 cctgcacgac caaaatcagc ttctgttgtg ccttgaacag ttgtacggat ttcagctcct
     7561 tttgtatcta aaagaatacc gacatctttt cctgtctttt taactgcttc acggaccata
     7621 gtcatacgtg ataattgttc ttcatggtca ccatgagaaa aattaaagcg gaagacattt
     7681 gccccagatt caatcaattg agaaataatt tctactgagt tacttgctgg tcctaatgta
     7741 ctaacgattt tggttttttt catttaaaaa actctcccat tcaattttgt ttatttaaag
     7801 ctgtacacag cttaaatagc ttggtttctt tcactcttaa aatgaaagtg aattatttag
     7861 atcatatagt gataagtctg gtttatgttt gttgttttca agtgtatcaa tgatgtcaga
     7921 cgcaacgatt tcgttgtcat ggataccgac acataatcca ccttttcctt cttgaagcaa
     7981 ttcgacagcg taagcaccga atttacttgc caacacgcgg tcgcgtgcgc ttggtgctcc
     8041 accacggaca acgtgtccca agatagatac acgtgtatga tagtcaccaa attctgaaag
     8101 tttctcagcg aattcatttc cgcccattac accttcagcc aagatgatca agcaatgttt
     8161 cttcccgcga tcgcggcctt cttggatttt tttagcaact gaagccatat caaaatcatg
     8221 ttctgggatg atgatttcat ccgctccgcc agccacacct gaccataaag cgatgtctcc
     8281 tgcattgcgt cccattactt cgataacgaa tgtacgaaca tgagaagtcg ctgtgtcacg
     8341 aatacggtca attgattcta gtactgtatt gattgctgta tcaaaaccga ttgtgaaatc
     8401 agtcccagga atatcattat cgattgttcc aggaatacct actgctggga aaccgcgttt
     8461 tgtcaaagcc attgcgccat gataagagcc gtctcctccg ataaccacta gaccttcgat
     8521 tccgaatttt ttcaattgtt cgattccttt taattgacct tcttcagttg caaattctgg
     8581 ataacgtgca gaatacaaga atgtaccacc acgttggatc ttgtcaccta catctgccac
     8641 atcaagacga cgaatatctc cagcaactaa accggcaaaa ccataattga tcccgtatac
     8701 ttccagtcct tcgtaaatac ctttacgaac aacggcacga acagctgcat tcattccagg
     8761 tgcgtctccg ccactggtca aaattccgat gcgtttcata ataaacattc acctcattaa
     8821 aatactttat cctaagtatg acttaggttt acaaccattt ttgcgttaac cacgactaac
     8881 attctaacat tatttttctc acttgtcttg atttacggat aaaaaaatga aaaaaaatga
     8941 aagcaagtag tattttttac cgtaagttat ttgaaaacca cattatcttc gccaagcaga
     9001 taaataagtt gccgctttat actcttgctg tcatccaccc aataagcatc tgacagaact
     9061 acttttcgtt gcgttttttc gtgatacata actactggta tatatcctgg gtgtttctga
     9121 aaaatcactt gtaattgttt cattatctcg ggatcttccc gttcttctgt aatacgcaaa
     9181 tagcaagttt gtgaaggata ctgggcttct aactggttag gatcagcaaa gctttctcca
     9241 attagttgca gttcctgatt atatttgctg atttcgctcc gcccttgaac aaagatcact
     9301 cgatcaacgg caaacaaatt acgatattta cgataaagag ttggaaaaac tgtgacagaa
     9361 atttctccac tactgtcggt tccttcaaca aaggccatca gttctccttt ttttgtccga
     9421 atttctctaa cttccttgat catcactaat aaattgactg gttgattagg gacgatatct
     9481 gtaatcagct gaacttgttt agctaaacga agacgatcaa acccttctgc aggatgacct
     9541 gacaaataaa tacctaagta ctcctcttcc agcttcaagc gttcttctag tgtatagtca
     9601 gcgacttctt cttcttttaa tgccatgatt cccagcaagt caagacttcc accactgtaa
     9661 acgatatttt ggatttttcc ttcgagatca agcatcaatt gtttcctgtt aggagataat
     9721 tcatcaaaaa ctcctactgc tgtcagtgat tgcagtaact ctaatttcag ccaacgctta
     9781 tcgatccgta tgaggaattg atccactgac tggaacgggc cattttgttt tctttcgttt
     9841 aatatatctt ctacaaaatc acgccgaata ccttttatcg ctgtcagtcc gaatctgatt
     9901 tcttccttat tgaccaactc aaaactggaa ttactttgat taatggacgg cgaggaaagt
     9961 ttgagtttat ttttacgtgc ttctgcaata tattctttca attttgatgt gttatgccga
    10021 acagaattca tcaggctggt aaagaaagca cctggatgat gggcttttag atacgccatt
    10081 tggaatccca cgaatgaata agcaaatgca tgtgaccgat tgaatccata gtttgcaaat
    10141 ttttcgatat attcatatac ggtcgctgct gttttttccg aatatccctg ttgtaatgct
    10201 cctgatacaa aatgcgactt ttcttcatct aatacgtctt ttttcttttt actcactgcc
    10261 cgtcgcagaa tatctgactg ccctaatgta aatccagcca tagtcgaggc gatctgcatg
    10321 atttgttcct gatagacgat cactccatat gtgtttttca aaatcgggat cagtgaatca
    10381 tctgggtagc gaatggcttc ttttcctttc ttcctggcaa tgaaggtatc tatattttgc
    10441 atagggcctg gtcgatacaa tgcattgact gcggcaatat cttcgatgtt ttctggatct
    10501 aatcttcgta aaacgttccg gataccagca gattcaaatt gaaatactcc tgaggtttcg
    10561 cctcgttgaa acaagagcag cgtttttgca tcatttagcg ggatctcttt taatcggatc
    10621 tcataccctt ctactcgctt aacagctttc aaagtaaaat caataatcga taagttgcgt
    10681 agtcctaaaa agtccatttt caaaagaccg attgcttcta cctcattcat cgtatactgg
    10741 gtcagaaaag cttcatcaga accgcgttgg agcggaacta gttcaagtag attcttatcg
    10801 ctgataacta cacctgctgc gtgagtcgaa acatgtcttg gcaatccttc taaaatcact
    10861 gcagttttga ataaaagttg gtttttttcg cttgcctgaa ctaattctac taattgatgc
    10921 gaagttttat aagcttgggt caatgtgatt ttcaattgat tgggtattgc ttttgaccag
    10981 cggtttgctt cactttgcga taaaccaaac actctagctg catctctaag aaccattttt
    11041 gctgccattg tgccaaatgt tgcgatttgt gccatatgat attgtccata cttttctcga
    11101 acataattca atacttcttc acgtcgattg tcaggaatat ccaaatcgat atctggcatc
    11161 gtaaagcgtt cggggtttaa aaagcgttcg aacagcaaat catattctaa tggatccaca
    11221 tctgtgattt ctaacacata ggctactaat gaacccgcag cagaaccacg acctgcaccg
    11281 gtaacgattt tgtttctatg agcaaaagcc attacatccc atacgatcaa gaaataatca
    11341 tcgaatccca tttgatggat gacagataac tcgtagtcca aacgttcttg gtattccttt
    11401 gtatagtcag tgattcggcg ttccacttgc gaatagcata actctcttaa atattctcca
    11461 gcttgttttc cattaggaac tggataatgc ggaagtaatt tctgatggaa ctggaattca
    11521 aatgaaatcg attcagctaa acgctgggcg cctattacag cttgggaaag acccgctttt
    11581 tcgaatcgtt tgatcatttc ttgttctgaa agaagataga catttgctgt tagtggtaaa
    11641 gatgcagata aattttctaa aggtatttgg cttccctctt tgatatgttt catcacttca
    11701 agtgcaaaaa gttcttcttg ttgtaaacta gcgatgggat gtaatgcaca aagcgaataa
    11761 ttttctttta tcacatactg ataccattca ggcaattgat caggcgtgtt ttctgtcaag
    11821 ccgatataaa aatttctagt aaaaacattt tcaagtaaaa actgccaatg accgaaaaag
    11881 ttatcctctt tattgaaagc ctcagaaaca gaatgatttg ccggaacaac aaacgctaga
    11941 tggtcggttt ctgtcatttc tgcaagttcc aatttttctt ggatcatttt tcggctggaa
    12001 agtttcatca gctgctgata acctatttca tcttttgcgt acgcaagcaa gccataattt
    12061 ttttcttcat aagtataact aagatgtaat ccaatgattg gctgtactcc ttcttctaca
    12121 caaatacgat agaattcagg tgcaccgtat aagacatctt tatccgttat ggctaatgaa
    12181 gtgtagcctt gtttttttgc ttcttgtaca tattctcgaa tacgtatcgt actttgtaaa
    12241 agagaataag aagtaattgt ataaagttgt gggaaaaaca tcttgcattc cgcctttttt
    12301 ttcgttaaaa ttcactagcg ttaaaaggtt tgatcctttt actttcatta agatagcagg
    12361 cgctgaagtt gattattcca cttcatttgc ttgcttgtaa tctgtaggaa tgagttcttt
    12421 cgcaagaact caaattttat tgagatttgg tctcgtttct ttacattcaa agaaaatctg
    12481 gtaaaattaa gatagaaaag aggtgttctt tatgcgatat atcgttacat tattctgggc
    12541 tgtcgttcta ggtcaagtag tcggttatat tggtgctgca ttaacaagcg gaacatatga
    12601 ttttactttg acaacaatca tttcattcat tgcgggtgtc attatccttt tgattggtgc
    12661 agttgcgcca agaaaagaaa cctctgccca ttcgtaaaaa aacagccaag agcttgagat
    12721 ttctcaagct cttttttatt ttctactcct caccaccatt ataatctgct gcgaagaact
    12781 ttacgaaagt tgaataaaga aagaagaata tttgacagaa caaacaaact tgtcacaata
    12841 aaaacagcac tgtaattata agcatgcgcg actgtcgatc ctagcatggg acctaaaact
    12901 tgtccaaagt tgctaaacat ctgattgtag ctatagattc ggctgactcc ttcttgaggc
    12961 gtaatcttac tgatcagtgt attgacagaa ggcatcaaag cgccagttga aaagcctaaa
    13021 ataaagcgaa gtatacctaa ttggagcggt gtagtgacga atgccatagg taaataacag
    13081 caaaatgata aaatgagtcc tccaattaga actttttgat tcccaatctt atcccctagt
    13141 tttcctagtt tcggtgaaga aaagacagcg gaaacaccag ctatcgaaac aatcaagccg
    13201 ctgacaaaaa ggatattttc tgtacttccg ctcaattcac gaatatataa ggtcaaaata
    13261 gggctgatcg tagtgattcc tatctgtaaa atcaaggtcg taatgaaaag accgatcaaa
    13321 atagagagat gatccatttt tgaaaaaatt tctttggtgc ttatcagatc ttttttctcg
    13381 attggatgga aatcttcttt gactaagaaa atggtcaata ccgttgtgat catcaacaaa
    13441 gcccctgtga tcaagaatac attttccata ccaaaccact gagccagtgc accgcccatt
    13501 gaaggtccga tcagatttcc agcaatcgct ccggtagaca atgttcctaa cgcccatccg
    13561 ctcttttctc taggtgcctg tgaagcaatc atcgctgtgg catttggaat atatccagac
    13621 aaaatcccat tccaaaaccg catgatcaaa agccaatacg cgtttggtac aaaagccaaa
    13681 gatcccattg ttaatgtcat ccctgctgct gcacggatca tcatgagttt tcggcctttt
    13741 cgatcagcta gatttcccca gattggtgca accacggcag atgcaaaagc agtcacagag
    13801 attgctagtc ccgaaaacaa ttcgatttga tcttttggtg ttcctagttg ctcaacataa
    13861 actggtatga atggcataac taaactaata cttgcaccag tgaaaaaaca gccaatccac
    13921 gcaatcacta aattttttct ccaattaatc tccatctatc atccctcttt cgtctctttt
    13981 atcataccta ttttcgaata aacaagcaac gatctcgcca gtaaaaagag aaaaatcccg
    14041 caaaggtcat tttatatgta acgttctttt tatgtaaaac aaaaagagat cagaacaaat
    14101 actgttccaa tcccttctct ttagttaaat tgttgctaga atgatgaagt tgataatgaa
    14161 taaaatatca acgatccaca aagcaacaga aacttgattg tattttcctt tgattacttt
    14221 cacaattgta tagaaaataa atcctgctgc aatcccatag gaaatgctat agcacaatcc
    14281 cataaagatc gatgcaaaga aggccggtaa agcttcttcc atatctagcc atttgatgtc
    14341 tgcaaaagaa gccatcatca ttactccaac taaaatcaat gctggcgcag ttgcttgatt
    14401 tggtacaatg gcgatcagtg gagagaataa actacttaat gcgaatagca ctgccactac
    14461 tactgaagtc aaaccagtac gtccaccggc cccgatccca gctgcagatt cgacataagt
    14521 tgttgtattc gatgtaccaa agatagcccc cacagaagta gcgatcgcat cggcaaatag
    14581 tgctttatcc atttttgttt tgaacccacg accatcttcc aacgcgatct cgtcttcttt
    14641 tgagaaaata cctgtgcggc gtcctgtacc gataaatgtt ccgatcgtat cgaaagtatc
    14701 agataaactg aatgcgatga tcgtcatcaa tacttgagga atcttggaag aatcactgaa
    14761 taaagattgc atgccttcag aaccaaatgc tgcaccgaaa gttgttccta attctttaaa
    14821 tgaattacct aacgaattcg tttgccaatc aatggaactt aaatcaacaa cacccataaa
    14881 aataccaagg actgtggtcg ctacgatacc gataagaata gccccacgga cattttttac
    14941 aactaaaatg gctgtcagta ccaaaccaat cacagccaat aagataggag cgttgtcaaa
    15001 gttggctaac gcagggacga ttccaccatt tatcgtaaca tttgtcgctt ttccgccttc
    15061 aacgacagag ctcgaaatag ctgactggtc tgctgaaaaa gataagaatc ctgcattttt
    15121 gattccgaca tacgcaacga aaataccgat cccgccacca atcgcatgtt gaagactttc
    15181 gggaatcgcc cgaataatca tttttcgaat atttgtgaca gtaataaaaa tattgattaa
    15241 tccgcaaata aacaccattg ccaaggcttg ttgccaagaa taaccaagcc caaaaacgac
    15301 cgtaaaagtg aaaaatgcgt tcaatcccat accaggtgcc tgagcatatg gtacatttgc
    15361 gaacaatccc attaccagcg taccaataat cgatgcaatg attgtcgcta gaaatactgc
    15421 ttggaagggc atacctgatg ctgacaaaat cgtcggattt acgaataaga tataactcat
    15481 ggcaaagaat gtcgttactc ccgccattat ttcagttgat acatttgtat tgtttttctt
    15541 taaaccgaaa aacttttcca taaaaagttc cactccctga atttttttct gatggaaaac
    15601 tataataata aaaaataagt aaaatagggc aaatccatcg aacaatttca aattatatag
    15661 acgtttcatt cgcttttcaa cgttttttta acaaaaaaag cgaaaataat acttaacgtt
    15721 cgtgtttagc ttatgtttag ctatttatat agccataact gtagtttttg ttaggaatag
    15781 ctgattgctc ttgttaatta cctaaaatat gctagactaa aacaaatgct agttgaggaa
    15841 ggaaggaccc ttattgaata aaaaattaat tgccatcgat ctggacggaa ctaccctcaa
    15901 tcaagattcg atgattacat ctaaaacaaa agagactcta aaaaaagcaa tcaatgccgg
    15961 tcatcatgtc agtattgcta caggacgccc attccgaatg agccatcagt tttaccaaca
    16021 attagaatta acaacaccga tggttaattt taacggcgca ttagtacaca tccctaatca
    16081 gcactgggat ggtgaaagag aaacgttgat caatcgtgaa atcgcttttg aaatcttatc
    16141 gcaaaaaaag cagctaaatt tagattttat cgctgctgaa aatcgtgata ctttttttat
    16201 tgacagtttt gatttctttg atgaaaaaat ttttgcttct tctcgtccag gagagaagaa
    16261 tttgctttct cctaaaaatt tgacgacaaa tcctacatct ttgcttgtcc gaacagacaa
    16321 acgttttgca ggagctgtat ctgctgaatt aacacgtcaa tttggcagtt atgtcgatgt
    16381 tcggacatgg ggtggaccta ctgccatctt agagattgtt tcaaaaggaa tccaaaaagc
    16441 aaaaggcgtg caagagattg ctaactacct gtctatcgat caaaaagacg tcattgcctt
    16501 tggtgacgag cataatgatt tggaattgtt ggattatgca ggttggggag tagctatggc
    16561 taacggtact gaccaactca aaggcattgc aaatgatgtc actcctttga gcaatcagga
    16621 agacggttta gctgtttatc tggaaaaact acttaaacta taatagagaa ttagattcca
    16681 caaaaaagag gttgtgacaa tttt
//
LOCUS       DAZUMK010000051        16535 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000051 DAZUMK010000000
VERSION     DAZUMK010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.052"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(296..1024)
                     /locus_tag="ACG808_001588"
     CDS             complement(296..1024)
                     /locus_tag="ACG808_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5047216.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="ACG808_001589"
     CDS             complement(1002..2576)
                     /locus_tag="ACG808_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFS5047217.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="ACG808_001590"
     CDS             2805..3743
                     /locus_tag="ACG808_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5047218.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="ACG808_001591"
     CDS             3755..4666
                     /locus_tag="ACG808_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5047219.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="ACG808_001592"
     CDS             4690..6162
                     /locus_tag="ACG808_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS5047220.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="ACG808_001593"
     CDS             6213..6824
                     /locus_tag="ACG808_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS5047221.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="ACG808_001594"
     CDS             6854..8767
                     /locus_tag="ACG808_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HFS5047222.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="ACG808_001595"
     CDS             8895..11876
                     /locus_tag="ACG808_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HFS5047223.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="ACG808_001596"
     CDS             11873..13534
                     /locus_tag="ACG808_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-GlcNAcase"
                     /protein_id="HFS5047224.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="ACG808_001597"
     CDS             13574..14506
                     /locus_tag="ACG808_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFS5047225.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
     gene            complement(14898..16445)
                     /locus_tag="ACG808_001598"
     CDS             complement(14898..16445)
                     /locus_tag="ACG808_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFS5047226.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
BASE COUNT         5294 a         2686 c         3292 g         5263 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagttgaaa
    14821 agtaagcgcc ccatagaaca gtacctgtac gtcaaataac ccgacctata gaatagaatc
    14881 gggaatgttt ggcgttttta gttacgagaa aaatgttgtt gtacatcctt gttccaaggc
    14941 aacagttgat ctaaagtttc tgctgaaagt acgggaagat ttggtatctt atcaaataga
    15001 aactctagat actttacagg attcaatccg ttaagttctg ctgttttata tacactcaga
    15061 atgattcctg aagttcgtgc tccttcaaga cttgtggaat gcaaccaatt tttccgtcct
    15121 atcaccagct ctttgatatt tcgctccgca cgattatttg tgagttctaa acgaccatct
    15181 tcaaaaatag gttcaaaacc acttcgtagt ttacacgcat attgaacggc tgcatccaac
    15241 ttggattttg caacaggatc tatcgttgcc atccactcat aaaaagcaat aaataatgga
    15301 cgcagttctt gttgccgaca gcttaatcgc ttctcaggag aaaagtcttt ccatttcttt
    15361 tctaggacaa accatttatc caattgtttg actgcttgag ctgcgggaat atccgtgttt
    15421 ttctttcctt ttggaattgc ttcgaaaaat ttccgccgaa catgcgcgaa acaatagaca
    15481 agtcgtacac tttccacggc attatacccg ctatagccgt cacagtgtaa ataccctgta
    15541 aactccttga gaaaagtttt cggaacatct ccagcacgac tttcgtcgtg gtgataataa
    15601 acgatgggat gctgactgtc ttttcctgta caaaactgcc agtaataggt atctgttttg
    15661 gcactttcaa tcacacgata acttgtttca tccccgtgta gaatctcttc ttgattaaga
    15721 tagactctta atcgttctcc taaagggtct aacgcgtttt gcactgcttt aatatgccaa
    15781 ttggtgatat tatctctcga aatatccaac ccatgtgttt gtttccaata gttctcttgc
    15841 cgataggcag gaactttctg accaaatttc atccggatcg tttccgccac taaactattg
    15901 gaacctaagc tattagaaat caaaggttta ggaacgatcg cttttttaaa agaagaaagt
    15961 ccttcttttt cgcagctttt acaacaatag gtatgttgat aatgggtgag acaatccaac
    16021 atagcctgat ggaaacgaat ttcttctcgt acttttgtcc gtccgatatc tttcatctgt
    16081 gaaccgcaat gttcacaaaa ttgttcttcc aaaggaagga gatgttcttt ctctgttata
    16141 ggtaagtgtt tgattttgtc tgctttcgtt cctacaactc gttgatgtcc ttttactgga
    16201 ataatggtgg cagctgcttc tggaacactt acagaaattt cttcatcact aaaaagattc
    16261 aattgcccat tcgtttcctc aaagagagtc tctttagagc gtccaaataa tttgttggtt
    16321 aaataattga ccttttcatt caatagttgg atttcagttg taagattctg aatggtttgt
    16381 tgttgtgctt gattggtttt atgggcttct tgaagcaact gaagcaacgt atctgtagtt
    16441 tccaaaattt ttcaccacct tgaatcgaac tgattttgtt acagaatacc acaagaaacg
    16501 cacaagaaat aggggttcaa cacatttttt gtgtt
//
LOCUS       DAZUMK010000052        16376 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000052 DAZUMK010000000
VERSION     DAZUMK010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.053"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(153..551)
                     /locus_tag="ACG808_001599"
     CDS             complement(153..551)
                     /locus_tag="ACG808_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFS5047227.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(548..1321)
                     /locus_tag="ACG808_001600"
     CDS             complement(548..1321)
                     /locus_tag="ACG808_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFS5047228.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1318..2001)
                     /locus_tag="ACG808_001601"
     CDS             complement(1318..2001)
                     /locus_tag="ACG808_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFS5047229.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2168..3055)
                     /locus_tag="ACG808_001602"
     CDS             complement(2168..3055)
                     /locus_tag="ACG808_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5047230.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3052..3921)
                     /locus_tag="ACG808_001603"
     CDS             complement(3052..3921)
                     /locus_tag="ACG808_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5047231.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4058..5344)
                     /locus_tag="ACG808_001604"
     CDS             complement(4058..5344)
                     /locus_tag="ACG808_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5047232.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5617..6195
                     /locus_tag="ACG808_001605"
     CDS             5617..6195
                     /locus_tag="ACG808_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS5047233.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPMKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6195..7124
                     /locus_tag="ACG808_001606"
     CDS             6195..7124
                     /locus_tag="ACG808_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5047234.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7121..8857
                     /locus_tag="ACG808_001607"
     CDS             7121..8857
                     /locus_tag="ACG808_001607"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS5047235.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8920..9756)
                     /gene="pheA"
                     /locus_tag="ACG808_001608"
     CDS             complement(8920..9756)
                     /gene="pheA"
                     /locus_tag="ACG808_001608"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFS5047236.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9753..10262)
                     /locus_tag="ACG808_001609"
     CDS             complement(9753..10262)
                     /locus_tag="ACG808_001609"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFS5047237.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10255..11550)
                     /gene="aroA"
                     /locus_tag="ACG808_001610"
     CDS             complement(10255..11550)
                     /gene="aroA"
                     /locus_tag="ACG808_001610"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFS5047238.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11563..12654)
                     /locus_tag="ACG808_001611"
     CDS             complement(11563..12654)
                     /locus_tag="ACG808_001611"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFS5047239.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12671..13837)
                     /gene="aroC"
                     /locus_tag="ACG808_001612"
     CDS             complement(12671..13837)
                     /gene="aroC"
                     /locus_tag="ACG808_001612"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFS5047240.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13839..14903)
                     /gene="aroB"
                     /locus_tag="ACG808_001613"
     CDS             complement(13839..14903)
                     /gene="aroB"
                     /locus_tag="ACG808_001613"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFS5047241.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14936..15958)
                     /gene="aroF"
                     /locus_tag="ACG808_001614"
     CDS             complement(14936..15958)
                     /gene="aroF"
                     /locus_tag="ACG808_001614"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFS5047242.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECALMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         4727 a         3620 c         2910 g         5119 t
ORIGIN      
        1 agcatatttt gaaaagaaag tctgatgctt agagtagaat ttcataaaaa tttactattt
       61 cataagtatt cacgagaaaa atgcaaacaa aaaagcggaa ataacgctca tccgccttct
      121 tgtttgtgtt tatttcctct tcaaacgctt gtctatgatt cagacttttc tctatcgtta
      181 ttttcatcta aaagttcttg cattccgata atttctcgat attgatgaat cttagtgata
      241 ccaatgactt tgtctacatc aatactaaaa caaataccat tacctggaat atcgatgttg
      301 atgccttgac caatcgcatc cattacttta ttcgtcaaac tatcgggtac taatgtcaac
      361 acaatatctt tttctggcga aatgtccaaa cctaaaaatt tccctgaatc atgtacacca
      421 gaacctcttc catgtaagat cgttccgcct gctgcacctg cttcttttga aaagtcgata
      481 acatcttcgc ccattcctcc gtcaacgatc gtcacaatca tgtgaagatt caatgctaca
      541 agatctttca tttgctttcc cccaattttt gcaatctttc tttttcgatt ctgtcttttt
      601 catcttttcg tttataaata tatcctagta ctaaagtgga taatattggt gtcaatgcaa
      661 tcattgcaac cacaccaaac ccgtcaatga tcggatctct tccttcaatt tgtgcagcga
      721 ttgccacgct cattgataaa ataaatgtcg aacacatcgg gcccgttgct actccaccgt
      781 tatcaaaagc cattgctaaa aagatcttgt ccacttttcg tcctaaaata aatgctagcc
      841 cgtatcctgg gatcaagaaa taataaagag agaatccgac gagcaaacgc cacatagaca
      901 atcctactgc tgcgccgatc ccaattgaaa ttaccatcag catgattttc gatcggattc
      961 tcccctcgct caattcttca acctgtttga tcattacatg aatcgccggc tcagcaaacc
     1021 ctactaagaa ccccattaaa aagccaaggg gaaccagtat ataattgtaa ggcaatcccg
     1081 caatagcact gcctaaatgc tgtcctaccg gtacataagc catattgact ccgtgcagga
     1141 aaaggatcaa cccaatagtc gttatcaaga aacctttcat aatgtcaaag aaacgctttt
     1201 tgcgcatacg aaaactaatg atattcatta aaacaaatag gattacgata ggtgccactg
     1261 ccaaaatgac ttccatggta acgatttcga ttccatctat cacttgctgc cagaaactca
     1321 ttggaagatc acccccaaaa tcatcacggc aagaatcggc cctaacgaag caataccgac
     1381 cattccaaaa ctgtcatttt catttttctt ttgtttgatc atactagtca tccctccggc
     1441 aagggccaag ataaatggaa cagttactgg accagtagtc actccccccg aatcaaaagc
     1501 aatcggcata aattcggaac tggtgaaaaa tgaggcgatc aaaacgccta tgtagcctat
     1561 agccatcaat tgataatagg atagcttgaa tacgacccga agcagcgcaa aggctagaaa
     1621 tactccagtg ccgacgctga cgatcccaat cagcaacact ctgccaatct ctccgtcaga
     1681 tatatcatat acttgttgtc ccaaaacttg cacggaaggt tcagcaatcg tgatcacgat
     1741 cccgatagca aatcccaaac tgaccaaaac ggtcaaactt ttctttttga tcatatattt
     1801 ccctactaga tctccgactt ccatcataga ataatctgct ccaaacaaaa agagcgtcat
     1861 tccgatcatc atgattgccg caccaaccag aaatgaagcg aggtcttctc cttctaacgg
     1921 tgcaaaaaca aacgttaaaa tgacaatcag gacagtcatc ggcaatatag cagcaatcac
     1981 ttctttaaaa ttttttagca tgtatcctcc tcctttactc tttaagttta ccttaaaatg
     2041 aaagagtttt cgagcacaaa cttaaaacgt tatcatttaa tttttctcaa cataaaataa
     2101 cacacgtccc aaaagtatct ttttacttgg aaaaatgact ttgaaggaac gcgcttattg
     2161 tgttttttta ttttacagag ccgatcattc cttgaacgaa ttgcttttgc aaaacaaaaa
     2221 atacgatggc agttggtaaa gtagcgatga ctaatccagc catgatcatt ccatagtctg
     2281 gtgtatagga agcacccata gcagataaaa caagcggaac cgtccgttta tcaggagatt
     2341 gcaaagctac taagggccat aaataattgt tccagctact catgaatgtg atgattgcag
     2401 ccgcggcata ggtattttta gccgtcggca tatacactct aaaaaaaata cttagttctt
     2461 ttagaccgtc taatcgagcg gcttctacta gatcttttgg aaaagattta acattttgtc
     2521 gaaagaagaa gatcaaaaaa gcagtgctaa cagatggcag gatgacaacc aagaaactgt
     2581 tgattcccaa aaccgttccg ttcaattgtg aaaacattcg atataaaggg atcatcaatg
     2641 ccgcaaaagg aaccatcatc gatagcagta gaatattgaa aattaggtct tttcgtttac
     2701 tttgataaat ctcaaatcca tatcctgcca ttgacgaaat cagcaatgca aacacagtgg
     2761 tcaccaaagc gatcatcgct gaattccaaa gggaacgagt aaaattcagc tcattggaaa
     2821 acaaattttg aaaattgatg atcaagttac tgccaatctt tatttttccc gatgtgatat
     2881 ccacgggtgt attcgtcatt cccaaaacca tccagacaaa agggaaaatg gaaataaatg
     2941 ataggatgct taatgctgta tactttaata ctgacccaat tgtcgaacga agttctaatg
     3001 cttgtttttt gacgttgggg cgttcgattg tttctttttc taccatattt ttcatcgact
     3061 ttctcctcct actttcgttt ggattatcga caatatcaca ataaaggaaa caataacaaa
     3121 ggaaactgct gccgcatatc caaaattagg cgtgaattta taactcaaat tatagatgta
     3181 ttgagatagg gtggtcgttg catttgctgg tcccccgcca gtaatatttt gtacttcatc
     3241 aaaaagctgc aatgtcccga tcgtcgaagt aatggaagta aacaaaatga ttggttttaa
     3301 atttgggatc gtcactttga taaactgctg ccatttagaa gcaccgtcaa tagatgctgc
     3361 ttcataaatc tccgaatcaa tattttgcat gcctgaaagg aaaaaaatca tattgtatcc
     3421 agtccaacgc caagtgatcg aaagaatgat cagaactctc gcccaaattg gatgagtcaa
     3481 ccagccaatc ggtgtgtcaa tcataccaat gcccaacaac aagttgttta caaggccaga
     3541 ttgagaaaat agacttttca tgatcagaga ataactgacc atcgaagtga tacatggaag
     3601 aaagatagcg gttcgaaaca accctttata tttcaacttt ttatcattta aaaggttgga
     3661 aataaaaagt gctagaaaca acatgatcgg tacttgtaca agtaaataca ggaatgtatt
     3721 aaaaaaagct tttttaaaag ttgcatctgt caacatccgc tgataattac ctaacccgtt
     3781 aaatgtcata ttgacagggg gaccactttg aaaagacgtg atcaaagcca aaaccatcgg
     3841 aataaaaacc atgattgata tcaaaatgac tggcaagatt aaaaaagcat gcccgttttt
     3901 gaataaatct ttctgcttca ttcttttccc tccaattcta tcattgatta tctacatgaa
     3961 aagacaagaa agacaatatc tgtaaggcag acgtgatctt ttttgtcttt tcgtttgtct
     4021 gatgaaaaag cgaagaaatt cactttttta ccaccgatca attagccaat tgtgcttcta
     4081 cttgtttttg tgtatctgct agtaccttgt ctgtctcttc tccattgatg attcgatgaa
     4141 gagcttcagc tacgactgct tcaattgcat aggtctcatg tccgtaattc acttctggta
     4201 tttcagatgt ccatgttgaa aaatcgtcaa agatcttttg accactgtaa aattcagaag
     4261 cttcttgata agcttcttgt tcattcgcgc taagcatcgt agatactagt ccgatttctt
     4321 tcgcaagagt gcccatcagt tcttcattcg aagcaaacgt tgcttctagg aaatctttcg
     4381 ctgaatcttc tccagctact cctttgatca cataccagcc agcgccgcca aggttagaag
     4441 cttgcgcttt ttgcagattt tctggaagtg caggtattgg tgcaatcttc cattttccag
     4501 attggttttc tgctccttgg atcgttgaag aataccaagc gccagttggg ctagaagcta
     4561 ctgctcctga ttgtatcgca gttactccag cattccagtc agaggtttgt tcgactagat
     4621 cttctttcaa aagagtggca aacaattcaa gaccatactt taaagatgca ttgtcttgga
     4681 tcgtgactgt ctccccatct tcagatgtat accattctcc tgcctgttgc ataatcatcc
     4741 ttactcgacc aaggtcagaa ggattgactt cagcgatttt ctttcccgtc ttttctttga
     4801 catctcgcgc tacttgaata tactcgtccc acttcagatt ctccatatcc tcttctgtat
     4861 aacctgcttc tgccatcaga tccgtacgat agaagtttgc tgttacgccc gaatcgaaag
     4921 gaacaccgta tattttatct cctactttgt ttaccgcaaa tttatacggt gcaaaatgat
     4981 cttcctttac aatagcagac aaattagcga atgcatctgg ataagaagtc aaatagcctt
     5041 gtatcctata atcttcaatc aagacgatat tgggcaatcc ttctgtattc ccgctcgcta
     5101 aagcggtgtt cagtttctga acgatatcat cctgagacat cgtaacaact tctatctctg
     5161 tttcttcgtt ctcataaact ttttttgctt cattcactgc tttgatattg aatgtctcat
     5221 cccacgccca gacagtgact ttttcttctt tgtctgctgt actactagca cttccgcatc
     5281 ctgttaagaa acctgatgct gccgctaaac aaacgactcc agcaattaac tttctgatgt
     5341 tcatttttat ccgctccttt tccatccatt agttgtttac gtttgctaca ttcatcatac
     5401 caaaatttga aaacggtttc tttattattc agatacttca aaaatttctc ctaaaataat
     5461 aaaaaaatac aaatcaacta tgacaaaagg aataatctta gcatctagac caaagatcga
     5521 aatggtgagg tttctttaaa ataaaaatat ccttgcattt tctcctagaa ataagtattt
     5581 ttaaattaca agactattct taaggcgggt aaaaaaatgc caatacgcgt aattcttatc
     5641 gatgatgaac agacgatttt agatggaatg aaacagttga tcaattggtc tgagcacggg
     5701 tttcaattaa tagggacttt ttccagtccg atgaaagcgc ttgatttttc tttggtacat
     5761 gcaatcgata tcgtggtcac agatgtaatg atgcctgaat taaatggaat cgaattatcg
     5821 cggctattaa aacagcaaca acctgaaaca cagatcctta tcctctctag ctatgatgaa
     5881 tttgatatgg ttaaagattc ttttaaagaa ggcgtcgctg attatctttt aaagcctcga
     5941 ttgagtcctg atttttttct aaattcctta actgccgttt cacaaaaaat caaaaaaaag
     6001 cccaacaaaa gcccaatcac caatcgttat gagcagatct gcgagaatct ttcacagcag
     6061 attgctggac aaactcgtcc attgcttgac cctcttcttt ttgaacacac cttgtttttc
     6121 tgttatatac cgaaaaacgg ccattcagtt caaaacaagg ctctaaacaa aaacagtcga
     6181 tcacccattc attgatggat agcgacgatt cgtttactat ttatcctgtt tctacctttt
     6241 cggaagaatc cggctatgta atcaatactg attccaaaga tacacttagg aagtttgttc
     6301 aaaagtttcg tcaagaagtt tttccaggtg atttttttgt tgtttctgat tcttttgcaa
     6361 tccaagatat caatgaaata ttccaagaga tcagaaattg caccaaagga caacgtttct
     6421 atcataaggg aaagaacctt gcttatcagc atgaacttct tccgttatta accaaccacg
     6481 agcagcaagc aagtagttat ttaacaaaaa tcgttcataa agactacgct ttagctgtca
     6541 aagaacttga gaatttcttc acgacgatat tggaacagcc tattgatcca gcttatttga
     6601 aacatgaggc aatcaatcgt ttctatgcat tactcaacgc tttggctgac gagcatgttg
     6661 ggaatgaaga actttctttg ctcaagatcg cccttccttc tttattagcc gatgctgagt
     6721 atttcgaaga tttttctatt cttgtcaaag aaacacttga gaaactcacc cacttttctg
     6781 caaatgcaaa aaaagacaat ttcgatatct tgactttgat ctacgagttc gtccaggaaa
     6841 attatgagaa agaaatcaat ttacaacttt tatccgaaaa atatcatttt tcatatagct
     6901 atctctcagc catctttact gaaaaatttg gtatcaactt ttcaaaatat ctaaaaaaag
     6961 tccgtatcag taaagccaag ctctttttga cggaaacacg gcttactttg acggaaatct
     7021 gtgagaaagt cggctatacc gaactgggtt atttctccag agtttttaaa gaagagacag
     7081 gaatgacacc ttctcaatac cgtaaaggaa cttttgtaaa atgagaaaaa aaatcaaaga
     7141 gcatgaactc tttgccaaac ttctgttcat tttgttcatc ggtctttttc tgcaagccat
     7201 cgtcatttct ttgtttattt atcatcgatc aagggatgcc tatatccagc tgttcaatca
     7261 atcgaatgat gtggtactaa aaaagattca atctgaattt gaaagtttga atgatacaat
     7321 cgagaataca ttagccgctt ttgattctaa tccagctgtc aaaagctatt tttccaatga
     7381 tccagcccaa catatggaac agcttcagca actaagaaca atccaaaaaa tgaatgactc
     7441 tctttcaaaa atccatccaa tgatcgatta cgatgtattg atctttggag aaaatggaag
     7501 aacttttgtc ggaaacgaca tgctcactgc cgtatctgcc gattcttttt ttcaatctgc
     7561 gattgctcag agagtgaatg aacgtgctgc tgatacacaa atgctttttg cacaccacgg
     7621 attgactctt cgcgataaga aagcacccag cgtcttcttt gtcaaaaaac tgaaaaatac
     7681 attgaatcac gtttacggat atgctgtttt atcgatctca tccaagcaac tagcgaatct
     7741 ttttcaatct gtagtaaatc ctgagatatc gaagatctcc ttcatgaacg aagaacagac
     7801 gatcatcgca tcaaatgaag aaaatacaat tgggagccga tcaatgattt tcaaaaaact
     7861 aacagtaggt gaaacaacga tttcaaatgg acagcagttg acccgtcttc ctctgtaccg
     7921 ccaggattgt acattactca gtcaagtcga tcttcattct ttatctaacc aaatgggcgt
     7981 gatttttccg attattttgt ataatatcgt gacacttgct tttgctggct cattggtatt
     8041 tatgtatttg gatcgacaca caaaatcgat ctaccgcttg attgattcgc taaaacggct
     8101 agaggaagat ggcttaaaag ctactgttcc tgtacaagga agccatgaag tccaattgct
     8161 agggaatacg atcaatcaat tgctaagtaa tgctcagcat cattatgaac agacgatcca
     8221 agacgaaaag aagaaacgtt cgcttgaaat ccaaactatg caagcacaga tacagcccca
     8281 ttttatttat aatacgctga ctagcttgaa atttttgatt tggcagaaaa aaacagatga
     8341 agcagttcaa ggaatcgaca gtttcattac tttactaaga agtacgattg ggaaaaaaga
     8401 agaagtgatt ccagtcaagg aagaaatcaa aagcgtacaa agttacatcg atcttttgag
     8461 tatgcgatat ggcgaaagaa tctcggctaa aataatgatt cctgatgaac tgcagtcatt
     8521 atctatgccg aatatgatcc ttcaaccaat tatcgaaaac gcctatcttc atgcttttca
     8581 caataaaacg caaggattca tcacggtgta cggcaaaaca aaaaatcagc ttcttttatt
     8641 cgaagtgatt gataacggcg atggcttcga tactgaaaat accaaacaaa aaaatgatca
     8701 tttttcagga atcggcatca gccatgtaga tgagcggata cgcctaatgt atggcgatgc
     8761 atatggctta acgatccaat cagtgattgg gacaggaacg actgtaacga tttgtctacc
     8821 gcttcttcct aaaaatcaat cagaatatca aaaataaaga atagccaagc gctgattttt
     8881 ttattttaag ttaaataaat caatcagtat tcttgacttt catctacgcg tctcttctac
     8941 tgtcagcact ggataacagc ccaatatttg actttctcct ccgagcatct tgatttcttg
     9001 gagcgcattc tctactaatg tcattggacg gtccaacaac aaatcgatca cgaaaaaata
     9061 ttctcccaag ctagttttca atggtcggga ttcaatctta cttaagttga tttcccgcca
     9121 gccaaaagct gccagcattt tatgaagcat ccctggccga ttagcaggta gtgtcaagat
     9181 gacactcatt tttactggag ctgactgctg gctggtcatc tttcggtcac cgacgatcca
     9241 aaaacgtgtc tggttcaact cattatcttg gatattctca gctaagatct ccaatccaac
     9301 atgctgtgct gtttctaatg aagcaatcgc tgctgcgtct tctttcggat gttctgctac
     9361 atacattgct gcagcagttg ttgattctgt tgctactaaa ggaacattag gataatgcgt
     9421 ttctaaaaac tgttgtgatt gtgctagtgc ttgtggatgt gaaaggattt ttgtaatctt
     9481 tgttgctgga ttgcctaaaa gctgctgctt gataggaaga acgatttcac ttttaacttc
     9541 gacttctgga tgtttggaaa gaagatcgat tgtgtgatga acagctcctt ccaatgaatt
     9601 ctctacagga acgacagcca gatcgacttg tttgcgaaac aaagcgcgca aacaaacagg
     9661 gatggaagca tacgcagtca attgttctgt aggaaatagc tgacaagccg cctgataagt
     9721 gaatgaactt tctggcccta gatagctgac gttcatatgc ttttgatcct ttctataatg
     9781 gtatcgatga tcttttctgg tgtttgattg gtcgtgttga ttcgatgtgt cgcactttct
     9841 tcataccaag aaagtcgttg tgccaataat gcttttattt cttctcttgt ttttccaaga
     9901 gctaaaggtc gaatagacgt ttcatcttgt tggatacgat caactagaca gtccgcttct
     9961 gcctctaagt aaagaacaat cggctgtgtt tttaaaaaac gccgattttc tgcactttgg
    10021 acaatccctc caccagtcgc gataattttg tcttcatttg acagctttct taaaatatct
    10081 gactccactt ttcgaaagct tttttcaccg taatagtgaa aatattcggc aatcgacaat
    10141 tggatatgtt cctcaatctt tgtatctaaa tctacatact ctttttgtaa acattgagct
    10201 aaccctttac cgattgttgt ttttccagcg cccatgaaac caatcagcaa aatgctagcc
    10261 atgagcttct cctcctgaca caagttctgc taaatcgtca aaaaatgccg gataagagat
    10321 tgaaacagct tcagctcctt ctagttcgca aggtccatca gctatcaaag cagctacttg
    10381 cagcatcata ccgattcgat gatcgcctct gctggacact tttgcgccat gcagaggtgt
    10441 cggcccatgg atgatcagac cgtctgctgt cgcttttatc tttgctccca ttttctgtaa
    10501 ttcttctgcc accgcatcaa ttctgttggt ttcttttact tttaattctt ctgcgtcttt
    10561 gatgatcgtg attccttctg cttgagtagc cactaaagct agaatgggca attcatcaat
    10621 cagacgcgga atgatttctc caccaatcgt tgctttcttc aaatgactcg tcttgacact
    10681 taaatcagcc gattggttct gttcattgtg attcgtctgg gtaatcgctg cgcccatctc
    10741 ctccaacaca tctaagatac cagttcttgt tggattgacc ccgacatttt ttaacaacag
    10801 ttggctttcc ggaacaagaa gtcctgctgc tagaaagaaa gcagctgatg aaatatcacc
    10861 tggaacagtt atctgttgac cagttaattt ttgcggtccg gtcactatga tcgttttatc
    10921 ttccactgtg atccttccgc caaattgacg aatcatttct tccgtatggt tacgggaacg
    10981 ttctttttca acgattacgg atgtgccttc tgcttgtaat gccgcaaaca gcaaagcaga
    11041 tttgacttgc gcactggcta caggcatatt gtatcggata ggtgataaag agcgggttcc
    11101 tgtcacagtg attggtagat actggtcatt ttcttctcct tgcaagtcag cgcccatctc
    11161 gcgtaaaggt tccatcactc gccccattgg acgtttagac aacgaagcat ccccgaacag
    11221 agtagtcgta aaaggctgtc cagccaaaat acccatcaat aaacgtatgg tcgtaccgga
    11281 gtttcccatg tcaattgatt cggttggttg gacaaatgag tggctaccgc gaccatgaat
    11341 cttgatcacc tcttcctctt cttcgatctc gacacctaat tgttggaagg catgtaaagt
    11401 actcagacaa tcttctgcgc gcaaaaaatt ttgtatagtc gtcgttcctt cagcaattgc
    11461 tccaaacatg atgctgcgat gggaaattga tttatctgct ggtatcctaa cagtccctct
    11521 taatccatgt atttgttgta ataattgcat ttatccagcc tcctatttga tccagcaatg
    11581 ataactggta ttagacataa tgattctttt tgcttgtaac aaatcctgtt ggtttttaaa
    11641 actgatttgc agtattccta aaatatcttc cctggtctct tggatcttca aattaatcaa
    11701 agaaactccc gcttttccga tcagtccagt tacttctgca ataacgccag gttcatctgg
    11761 cacatcgata aataaatcaa aaaaggcagg aatcgcgcct tgcttatgga caggcaactg
    11821 gtctcgactt tcttttgccc gattgaaaaa agaaaagatt tccgattggt tctcagtcat
    11881 gatccattct gctatttgtg tcatttcttg ttgccaatct gccaatcgtt ccaataatat
    11941 ctgcttattg ctcaacaaaa tatctgtcca catgacagga tctgaggaag cgatcctagt
    12001 gatatctcga aagcctcctg ctgccagttg tttggctcgc ggatactctt gattgaattg
    12061 ttctgcctga ttgactaagc cagctgccag aatatgtgga aaatgactta gcattcctgt
    12121 tatacggtca tgttctcttg ctgtaagagt tacatatttt gctttggttc cgcaaaatag
    12181 ttcttgcagt ttttcaacat cttttttcgt cttttccaaa ggtgtaaaga tgtaataggc
    12241 attttcaaat agtttagcat ctgctgcctg tatacctgat ttatgagaac cagccattgg
    12301 atgtccaccg acaaaccgaa gatttttctg ctcagcggat gtcacgactt gctgcttcgt
    12361 actgctgaca tccgtaatga tgacgtgatc agacaagttg attctttcaa ggacatcaag
    12421 ataagctaag gttgtacgga cgggaacggc taataagata acgtctgctt gttctgcacc
    12481 tgtttcaaaa tcacttgcaa cgtaatcaac gatctgctgt tctactgctt gctcacgggt
    12541 ttttgactga ctatcccaac caactactat cagttctgga ttggtctgac gaatacaaag
    12601 tgccatagaa gcaccaatca gtccaagccc cacgataaaa acatgttgtg ccatcttatt
    12661 tgcccccttt ttagtaatgt ttcaagtagt cacggtactc cttcactgca gctttcaact
    12721 caacaagcga atcacttggg aattttgaca atagctctgt cgctaccaca gtggctacaa
    12781 cattttccgc aacgacgcta gctgcaggaa ctgctgtact atccgaacgt tctacacttg
    12841 ctttgtaagg ttcttttgta tcgatatcaa cactttgaag tggcttgtac aatgtaggaa
    12901 tgggtttcat gaccccgcga atgacaagtg gttcgccatt ggtcattccg ccttcgaagc
    12961 cgcctagatt attggtacgt cggaaatagc ctctttcttc ttcccatagg atctcatcca
    13021 tcacttctga gcctggtaat gaaccagaag tgaatccgac cccaaattct acgcctttaa
    13081 aggcattgat actaaccaca gcttgtgcga ttttggcatc tagcttcgtg tcccattgta
    13141 cgtagcttcc taatccagct ggaacgcctc caacgagcac ttctacaatt cctccgatcg
    13201 tatctccatt tttctttgtc tgatcgatca aatcttttat tctttgctca actgctggat
    13261 caacaactcg tacttctgac gcttcagaaa tttctttgat ttcttgaatc gagatatttc
    13321 caggtatttc tgcttggatt ccaccaagac aaatcacgtg tcctgctacg atgatgtcaa
    13381 gttcttccag aattttcttg gcaattgcgc cgattgctac acgcatggtc gtctctcttg
    13441 cagaggaccg ttcaagcaca ttccgaagat ctcgatgatg gtatttcata ccgcctacca
    13501 aatccgcgtg tcctggacgc ggacgtgcaa ctcgccgcag tgattggtct ttttcaggca
    13561 cttctgctac agacatgaca gttgtccaat ttttccaatc tttattttct accactaaag
    13621 tgacaggtga accaattgtt ttgccgtgac ggatgccaga cgtgatccga acacgatctt
    13681 tttcgatctt catccttcct cctctgccgt aaccagcttg tctgcgagcg agttcgtgat
    13741 tgatttcttc ttctgacaga ggtaatcctg ctggtagtcc ttcaataatg gctgttaatt
    13801 ctggtccatg ggattctccc gcagtcatat aacgcatctt acagtcctcc taagtaatct
    13861 ttgaatgtct ctgtgggaac aggaacgatt tttgcttgcc caatcttttc tagcaagatg
    13921 atattcaatt ggcttcctct tgctttctta tcatgggtta tcgcgtggaa taattcatct
    13981 ggatgactta gatcgtacgt aatcggcaga tggaattttt gaagtatcct ttctaaagca
    14041 gctgtcgatc cagttggaga caagccgatt ttttctgcat gagaagtgat ggctaccatt
    14101 ccaatagcta ctccttctcc atgactgact accccatatc ctgctgtatt ttcaattgca
    14161 tggccaattg tatgaccaaa atttaaaata agtcgatttc cttggtcaaa gacatcttct
    14221 tccactactt ttcgtttgat ttccaacgta gcagtaatga ttggaacagc atgttccaga
    14281 agatcttttt cgtctttcag ctcgttcagc tgtttccaaa gcgttgcatc tgctatagct
    14341 gctgatttga tgatttcagc gattccttct cgtaaacgac gtaccggcaa agtttttaga
    14401 acctcaggat caatcaatac accagctggc tggctaaatg ttccgacaag gtttttcgct
    14461 ttgtttgtat tgactgctgt cttcccgcca atactagaat ctacttgtgc aagtagcgta
    14521 gtcggaattt gaacgaaatg gatcccacgc atgaacgtcg aggccacgaa tgctgctaga
    14581 tcacccacca ttcctccacc aagtgcaagt atcccatcgc tgcgggtaaa atcattatct
    14641 gctaaaaaat cgtataactc agctgcttgt tctaaagatt tactggtctc acccgccggt
    14701 acgattgaaa ggactggttc aaaattagct tgtctcaatt ggaacatgat tttttctgca
    14761 tatagtggag caacgtttgt atcagtgatc acagctactt tttgaggtct ccaaatcgtt
    14821 tgaagccatg atccagcctg ttctagtgcg cctttttcaa tatgaatatc gtatttttgg
    14881 ttatcaacat gtacagtaag cattcgtttc acttctttct tttaaaaagc tggaattaat
    14941 cgagtgtctg aatcttttcc attgcttctt tcatgatcgc aacttctttc atcatgcgtg
    15001 agtaagtctt ttcattcaac gattggggac cgtctgacca agcatttgca ggatcaggat
    15061 ggatctctac gatcatgcca tctgctccgc ttgccacacc cgcacgagcc attggtggta
    15121 caagttccca aattcctgta ccatgacttg gatcaacaat cacagggaaa tgagttaatt
    15181 ttttcatcaa tgggacagca cttaaatcaa acgtattacg tgtcgctgtt tcgtaagtcc
    15241 gaatcccacg ttcgataaaa atgacaggag atttgtcttc gactgcaatg tattctgcag
    15301 cattaagcca ttcattgatc gtaccggaaa ttccacgttt caatccaaca ggtttacctg
    15361 ttttaccgac agcagatagc agtttaaaat tttgcatatt acgtgcgcca atctgtagga
    15421 tatcgctgta ctcagccacc atatcgatat gcccttcatc catcacttca gtaatcactt
    15481 tcattccaaa ttcatcagcc gcttgacgga tgtatttcaa tccttcttct tctaaacctt
    15541 ggaaagcata tggagaagta cgtggtttga aggcaccacc tcgcagaatt tttgcgcccc
    15601 cggctttggc cattagcgca cattctctga tttggtctaa tccttcgata gaacaaggac
    15661 cagccatcgt cacgaaactg ccgtcaccaa tttttactcc gtctacatcc acgactgtat
    15721 tgccaggatg gaattctcga gaagtcaatt tgtatgtgtt taaaatacgc acagcatttt
    15781 ctactccttc atagcgtgta aatgctcctt cttgtaaact tcgcgtatca ccttttagtc
    15841 caatcactac tcgttctgtt ccttgatcga ttgagacttt gaatccttct ttttcgatat
    15901 gatcgacgac tttttttatt tgttcttccg ttgcttcttt tttcataatt actatcattg
    15961 tttttcccgc tttcttttgt ttacttatct atcttggctg attttctcta ctttttcaag
    16021 gtggttttac attaattcct actaaatgac cgtgtcctgt tgttctcatt ttcgctcctt
    16081 ctttcctact tatgacccag ataatcaaca aaaaaagatg tccctgtagg aaattgcctc
    16141 tacagtgaac atcttcaacg agccttaaaa agcgctgcaa aaatataaaa tagccactat
    16201 agatgaattt ctctacgtgg ctgtaagtcg tgtttaaaaa tctttagcca tcatagatac
    16261 aaacctggtt tgtttgtacc cacgaagtca tgaatacaaa cctatctaaa atagctaaag
    16321 taatacgtat tcagttttgt aagttttcta gatttcataa aaaatcgctc caaact
//
LOCUS       DAZUMK010000053        16322 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000053 DAZUMK010000000
VERSION     DAZUMK010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.054"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..962)
                     /gene="murB"
                     /locus_tag="ACG808_001615"
     CDS             complement(27..962)
                     /gene="murB"
                     /locus_tag="ACG808_001615"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFS5047243.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1035..1955)
                     /locus_tag="ACG808_001616"
     CDS             complement(1035..1955)
                     /locus_tag="ACG808_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS5047244.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2068..2820
                     /locus_tag="ACG808_001617"
     CDS             2068..2820
                     /locus_tag="ACG808_001617"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFS5047245.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3684..4217
                     /locus_tag="ACG808_001618"
     CDS             3684..4217
                     /locus_tag="ACG808_001618"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFS5047246.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(4707..5231)
                     /locus_tag="ACG808_001619"
     CDS             complement(4707..5231)
                     /locus_tag="ACG808_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFS5047247.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(5224..5697)
                     /gene="tsaE"
                     /locus_tag="ACG808_001620"
     CDS             complement(5224..5697)
                     /gene="tsaE"
                     /locus_tag="ACG808_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFS5047248.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(5820..6800)
                     /gene="pta"
                     /locus_tag="ACG808_001621"
     CDS             complement(5820..6800)
                     /gene="pta"
                     /locus_tag="ACG808_001621"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFS5047249.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(6953..7633)
                     /locus_tag="ACG808_001622"
     CDS             complement(6953..7633)
                     /locus_tag="ACG808_001622"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFS5047250.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            7759..8628
                     /locus_tag="ACG808_001623"
     CDS             7759..8628
                     /locus_tag="ACG808_001623"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5047251.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            8654..9169
                     /locus_tag="ACG808_001624"
     CDS             8654..9169
                     /locus_tag="ACG808_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFS5047252.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(9216..9779)
                     /locus_tag="ACG808_001625"
     CDS             complement(9216..9779)
                     /locus_tag="ACG808_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5047253.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(9834..10013)
                     /locus_tag="ACG808_001626"
     CDS             complement(9834..10013)
                     /locus_tag="ACG808_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047254.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(10090..10599)
                     /locus_tag="ACG808_001627"
     CDS             complement(10090..10599)
                     /locus_tag="ACG808_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047255.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(10695..11075)
                     /locus_tag="ACG808_001628"
     CDS             complement(10695..11075)
                     /locus_tag="ACG808_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5047256.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            11405..12154
                     /gene="pnuC"
                     /locus_tag="ACG808_001629"
     CDS             11405..12154
                     /gene="pnuC"
                     /locus_tag="ACG808_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFS5047257.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(12338..13615)
                     /locus_tag="ACG808_001630"
     CDS             complement(12338..13615)
                     /locus_tag="ACG808_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047258.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQQFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(13694..14338)
                     /gene="pcp"
                     /locus_tag="ACG808_001631"
     CDS             complement(13694..14338)
                     /gene="pcp"
                     /locus_tag="ACG808_001631"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFS5047259.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(14537..15481)
                     /locus_tag="ACG808_001632"
     CDS             complement(14537..15481)
                     /locus_tag="ACG808_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFS5047260.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(15483..16169)
                     /locus_tag="ACG808_001633"
     CDS             complement(15483..16169)
                     /locus_tag="ACG808_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFS5047261.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         4707 a         3372 c         2874 g         5369 t
ORIGIN      
        1 ttacttattt taagttccat tacttattaa ttggctaaac ttctgcttgt ttctgatatt
       61 gcttgttttt ctccaatgat ccgcacttct gtttctaaag acacatcaaa tttttctttg
      121 attacttctt ggatgtgcgc aatcaattca atataatctg tcgctgtcgc atgatcgata
      181 ttgacgatga atccagcatg tttttcagag acttgtgcac cgccccattt caggccttgc
      241 aatcctgctt gttggatcag ctgacctgta tagtgtccta ctgggcgctt aaagacactt
      301 ccgcatgaag ggtattctaa tggctgtttt gattgacgca attcggtcaa ctcatccatt
      361 cttgctttga ttgcttcata atcccctgtt tgcaaactga atcgagcttc caatacgata
      421 gcacgtaatt cttggacttt actgtgacga taagaaaata ccatttcgtc ttttgtcaaa
      481 gttttcaatg ttccatcttc aagcaaaaga tccacttcag caaaaacatc tttgatctct
      541 ccgccatacg cacctgcatt catataaacc gctccaccga cacttcccgg gatgccgcaa
      601 gcaaattcaa atccagtcaa tgattctgcc aaagctgcat aagtcgtatc aatcagtgag
      661 gcacctgctt ccgcaatgac catcgttcct ttgacatgaa tctgtttcat ttcagtcagc
      721 atgatgacta caccgcgaat cccgccatct tgaacgatca gattgcttgc atttcctaat
      781 accatccaag gtatatcatg gattcggcaa taatcgatga tttgctttac ttcttcttgt
      841 tttttcggga atgcaaggac atcagctggt ccacctgttt tcgtaaacgt atagttcatc
      901 aatggttcat caaacaatat agtaatcgtg gctaagcttt caactatttc ttctttattc
      961 actgaggatt ttcctttctt ttctcaaacg tcctcattat tctatcacgc ttaatacaat
     1021 cttgccagtc ttgttcaaag ttgatgacgg tagtgatctt taagcacttc ctcacataat
     1081 tcatgacttc ttacaacttc tttttgttta agcaattctg tttccgattt acctaaacgg
     1141 atctgagaaa agaaggcctc taccattggc tcaaacccgc gtttgactag cgtgtttgtc
     1201 cagtcgccaa attctttcac tttttttcca tcagcatctt gaatgacaag ctgggtcaga
     1261 tcgtttactt cataagtacc atttttactt gttacttgat atttttctat attagcgcca
     1321 cttgctaaat ccatcgttaa aatagccgtt tgattttgtg tttctaattg taaaatggca
     1381 tattccaaaa attcctttgt ttctcttatc tggtattttg ttctcaaaat gggttcatct
     1441 aataaataca cagctgtatc aataagatgt aaaaacaaat cataaaggac gaactctgtc
     1501 gtttcttttg ccgctattcg atttttttgc aggtggatca accgcttatc tgggatacgt
     1561 ttcaattctt ctaccatagg agcaaagcgc cggttgaaac cgatcatcaa aattatttgg
     1621 ttcttatcag ctaattcttg caattcacga acttcattta gatggacact cagtggtttg
     1681 tcaatataaa catggatacc attttctaga cattttttgg ctaattcata atgtactttt
     1741 gttgctgcgt ggatcataca tgcttcgatt ttttcttcta aaagctcatc gatcgtttca
     1801 tatagatgtt ggaacccgta ctctttttga attctttctt ttgtttcttt atttcttgtt
     1861 gcaaaataaa aatcaagtgt tccttgtttt ttcgcataag ttggtaaata tgctttttct
     1921 gcgatattcc ctacaccgat tacaccaatc ttcatctact ttcctcctgg atcatttagt
     1981 tactttcatc ttacaccatt ttttactaaa atgaggtaca atagtatcgc aaaggtaagg
     2041 aaattaattc gaaggagtgt gcttttattg aaatttatct cttggaacgt aaacgggctt
     2101 cgtgccatcg taaataaaaa ttttttagaa gtttttcatg agttagacgc cgattttttc
     2161 tgtctgcagg aaacgaaact ccaagcagga caaattgatt tagatcttcc aggttattat
     2221 caatattgga attacgcgga acgaaaaggc tattccggca cagcgatttt tgctaaaaaa
     2281 ccagcattga atgccactta cggaatgggt atcgatatcc atgatacaga aggccgtctg
     2341 attactttgg aatattccga tttctttctt gtaacctgtt acacgccaaa ttctcaaagc
     2401 gaattaaaac gtttagatta ccgattagaa tgggaagaag ccttttataa ttatttggaa
     2461 aatctaaaaa aacaaaaacc agtcatcgtg tgtggggatc taaatgtcgc tcatcaaaag
     2521 attgatttga aaaactggaa aacgaatcaa aaaaatgctg gattcactcc agaagaaaga
     2581 gcggctcttt ctcgactgtt ggataacggg tttatcgata cattccgtta tttttatcca
     2641 acccaggaag gtgtctactc ttggtggaac taccgattca attcaaggaa aaacaatgct
     2701 ggttggcgga tcgattactt tttgacgagc aaagatttag agccaggact agctgatgca
     2761 aagatccata cggacattat gggcagtgat cattgtccag tagaattaga tttgaaataa
     2821 agaggttgtg aaaaagtcgt ttagtttcga gtattaagaa aaattttgtg aaaatggctt
     2881 tccacatttt gcataaattt ggcataatac cgagaaactg gcttttgaac actgtttatc
     2941 gagagattgt gaaaaagctg tcctgcatca agtaataaga acagccaaac aaaaatagtt
     3001 cctcatattt ttcgtttggt tgggcttatt accgcagata caagcttttg aacaccgttt
     3061 attaagaggt tgtgaaaaag ctgcactgca tcaagtaata agaacagcca aacaaaaata
     3121 gttcctcata tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg
     3181 tttattaaga ggttgtgaaa aagctgcact gcatcaagta ataagaacag ccaaacaaaa
     3241 atagttcctc atatttttcg tttggttgga cttattaccg cagatgcaag cttttgaaca
     3301 ccgtttattg agaagttgtg aaatcagcgt cctgacctga gtattagaga aacaaatggc
     3361 aaaatagttc ctcatatttt gctatttttt gagctaatac cgagggtcag ctgattgaac
     3421 accgtttagt ggagaagttg tgaaaaagtc gcctactttc gagtcgtaag aaattttttt
     3481 gaaaatggtt cctcacaact ttgcataaat ttgacttaat accgagaagt catgatcccc
     3541 atctaactcc aaatcacaag gaatgagttc aaaaactagc ttttctcaac aaatattaca
     3601 taaaaaattc tatgtttttt gttaaattta tttgcttttt tcaaaagtat gttacagtaa
     3661 tttcccaagg aggttttaca gaaatgaatt ttgttgccat ggattttgaa actgccaatc
     3721 atcagcctta tagtgcttgt tctcttgcgc tagtcatggt aaaaaatagc cagatcgttg
     3781 atgaattcta tacattgata cagcctgaga ctcctttttt ctggcgaaat gtccaaattc
     3841 acggaatcca tcaagaagac gtacgcaatg cgccgaaatt cccagaagtc tggcaaacta
     3901 tccaaccata ttttcaaaaa aatcgtttag tcgttgcaca taatgctgct tttgatacaa
     3961 aggtcttagc gggatgctta gattattatc atcttgaaca gcctaattat ctttctttat
     4021 gtacagtgaa aagtagccgt cgcctgtttc ctgaatttcc aaatcatcga ttgaacactg
     4081 tgtgcgaaaa tttgaacatc caattagatc atcaccatga tgcattggaa gatagtcgtg
     4141 catgtgcgga aatcctgctt tatcaagaaa aaaaattcgg gacagatcca ttgaaaaaac
     4201 ttgtgactat aaagtaaaag aatacttatg aaacacacaa agaggtcgtg agcctgccgt
     4261 ttcactccga gtcacaagga gcaatttcaa aaaatagctt ctcatgttta ttaaatattg
     4321 tgactggtga ccgaggagct ggcaggagaa caccgtttat tggagaggtt gtgaaaaagc
     4381 tgttctgcat caagtaataa gaacagccaa acaaaaatag cttctcatat ttttcatttg
     4441 gttggactta ttaccgcaga tgcaagcttt tggacaccgt ttaccaagag gttgtgaaat
     4501 cagcgttttg acctgagtat tagagaaaca aacggcaaaa tagttcctca tattttgccg
     4561 ttttttgagc taataccgaa ggtcagctga ttgaacaccg tttattcaaa aaaaatggac
     4621 tgcgacacga cttttgtcac agtcccttcg tgtgtttttc tgccttctca aaaggatgac
     4681 agtttctcac tcttttccta tttcttctat tcaatcaaat aactcatttt atatacatct
     4741 aaaaattcac ctagatctgt tcttgcccct ctaggcatga ccgcttcgat ttggaagccc
     4801 atttttcggt aaaggtgtac ggctctttca ttacgaactt gaacgtcaag ctccaatcga
     4861 aaaagtacac ccatttcctt cgcccaactg atgatttctt ctagcataag agtgcctagt
     4921 cccattcccc aatattcctg cagaatactg ataccgactt ctcccacgtg agacaaacga
     4981 aattgactgt ctgccttgac agatgctgtt ccaataatcg tcccttcata gattgcaagc
     5041 agcaataaat tattattgct ttcgtatata taatccaact cttcttctaa ggtttctggt
     5101 ggtaacagga gttctgcttc atccaaaact aaaaattctg tctccttatt aacttgtccc
     5161 atcatcaaaa gaatgtcttt tgcatctcct ggcactgctt cccgcaagat gatcccaact
     5221 tcttcactca tgattttcag cccatttttc tagaattcgc tgtttgaagg tttcactttg
     5281 ctccccgtat gagattagtt tcacataccg cttttctaaa tctgtatcac ttttttctaa
     5341 aatcagttcc aaataatcct ccggcagtgc ttcttctaat agatttcccc attcaacaac
     5401 ggaaagccca tcaccttcaa aatattcatc taaccctaaa tctgctccgc tagctgctac
     5461 ccgataaata tccatatgat acaacggaat ccgaccttga ctatattctc taataatcgt
     5521 gtatgtcgga cttttgatca tttgcgttat ccctaatcct tgggcaattc ctttcgtcaa
     5581 cgttgtttta cccgcaccta gatcaccagt caatatcaga ttgtcacctg gcattgcgga
     5641 atgaccaatt gcctctgcga attgttctgt cattgttaat cctgatagtt cgatcatttt
     5701 tcatcttctc ccgctcttct ttctttttgc cagtatatcg cgtaatgaag tttttttaaa
     5761 gaaaaaatga actagtcaag aacagcagct gctgtttttg gctagttcgc gtgtatttgt
     5821 taatcaatca atgtttgtgc tgctgtgata atcgataatt tatatacatc ttcttcattt
     5881 gctccacgag acaaatcaga aaccggtttg ttcaagcctt gtagaattgg tccgattgct
     5941 tcaaagttac cgaaacgctg agcaatttta tagccgatat ttcctgactg caattctgga
     6001 aatacaaaga ctgttgcttg accagctact tctgaatctg gcgctttcag ttgtgcaact
     6061 gaaggaacat aagaagcatc aaattgcaat tcgccgtcga tcatgtattg tggcgctagt
     6121 tttttagcga ttttagttgc ttcaactact ttgtctactt ctggcgattt cgctgagcct
     6181 tttgtcgaga agctcatcat tgctactttt ggttcgatat cgaacaattc tgctgttttt
     6241 gcagaatcta cggcgatttc agccaattct tgcgcagttg gattcacgtt gatcgcacaa
     6301 tccgaaaaga catatttttc ttgatctctt ccacgtacca tgatcatcgc tccgcttgtc
     6361 cgactgatgc caggctttgt tttgatgatc tgaagtgctg ggcgaacagt gtcgccagtc
     6421 gaatggatcg ctccagagac cattccgtct gcaatcccca tatacgtaag catcgtacca
     6481 aagtaattca catcttttaa aatcttttct gcttgttctt tggatgcctt tccatttcta
     6541 cgttcaacaa aagtatccac catttcatcc cacttatcgt aattatcagg gctgatgatc
     6601 gtaaagctag atgttttgat tcctctggca tgcgctgcat ttcttatttc ttcttcgttc
     6661 cctactaaaa tgggttccac aagttcttct gctttcaacc gggcagctgc gcctaaaata
     6721 cgagcatcag tagcttccgg gaaaacgatt ttgatcccac ggcgaacaat tttaaatctt
     6781 aaactatcaa acaattccac aagatgacct ccagacgttt ttatatgtat tatcatacac
     6841 tattccagaa aaaaagaaca gtttatttta tctgtatcag tcaaaaatgc tggaaaaatt
     6901 aagtttttca catacaaagc gaactgcact ataacaggaa agcgctgttt tattacactg
     6961 tttcaggcag ttgccaatca atcggttctt ctcctaacgc aaggagcgca tcattcgttt
     7021 tcgagaaggg tttggaacca aaaaagccac gacttgcaga taacggactg ggatgtggag
     7081 atgttatgat gatatggcga ctcttatcga tcattttgat tttttcttgt gccggttttc
     7141 cccacaagat aaacacgatt ggtttttccc gttcatttaa tttttcaatg atcgtgtctg
     7201 ttaatctttc ccaacctttc ccgcgatgag aatacgcttg cccttcccga acggtaagaa
     7261 cggtgttgag taaaaggact ccctgtttag cccatgacac tagatttcca tgttgaacag
     7321 gtgcgattcc aagatcatca taaagctctt tatagatatt tcttaacgat ggcgggatct
     7381 ttacgccagg ttgcacagaa aaagataagc catgagcttg attttctcca tgataaggat
     7441 cttgtcctaa aatcaccact ttaacttctt cataaggagt caattccaat gcttcataga
     7501 tatgatacat atctggatgg atcttttggg acgcatattc tttttttaaa aattctctta
     7561 gatgcagata ataatctttt gaaaattctg aggacaaaac ttcttgccaa ctgttatgaa
     7621 tgatttcttt caaaaactcc acctcctaat aaaaagaata actcacattt tcttcaatca
     7681 caagggacaa tcttcaccct cagcacttga ttaatatggt aaactaggaa caagaagcag
     7741 aaaggaacga taatcattat gatcaaatta attgcatcag atatggacgg cacattatta
     7801 gatgcccata tgagtgtttc tcctgaaaat gccgaggcga tacgttttgc aaatgatgcc
     7861 ggtgttgagt tcatggtcgc tactggtcga aactatcaag aagctcgcgc agctttagat
     7921 gaggtaggaa tcgattgtgc gatgatcact ttgaacgggg cacaggtatt tgataaagaa
     7981 ggaaattcat tgtttaccgt gtcgattcca aataatcaag caatctctgt tttagatatt
     8041 ctagatgcta acggtattta ctatgaagta gcaaccaacg atggtcttta ttctgaaaat
     8101 caagcgaaac gaattgaaag ttttgcttcc atggtggcta cccatttacc tcaccttacg
     8161 tataaaatgg cgattgccat ggcctcagct aagctggagt tattgcacat cacatacgtg
     8221 gacagtattc gtgctatttt ggaagaaaag aatctggaag ttttaaaaat tatttgtttt
     8281 catacagaag ggccagctgt attaggtcca gttggaaagc agatttctct tttaggcgat
     8341 ctagccgtta cttcctctgg tcaaaacaat atcgaagtga accataaaaa cgctcaaaaa
     8401 ggaattgctg ttgcccatgt cgctcacgag cgtgggattt ctttagaaga agtcatgacg
     8461 attggcgata acttcaatga tgtcagcatg ctgcaagtag caggagtcag tttcgcaatg
     8521 ggaaatgcag aaatcgaagt aaaagattat gccaaatata caacggacac aaaccttgaa
     8581 tctggcgtag gaaaagccat attgcgcgcg attgcagaag acttgtagtt ttatctagaa
     8641 aagagggata tagatgtcag aatttttcat tcaggaaaag cagcttggac gtgcaactcg
     8701 gacaattgtc aaaaacgaaa aaggacattc cctgtttttg atggttggtc gctggggaac
     8761 aaagggcgat gcattgtctc tttatgccat gaatggcgat ctcgttgctc acatcaagca
     8821 gataagcctg acattcggta cgcgttttga gctgtataaa gagttcgaaa aggtgggaac
     8881 catgcagaaa atattcaatt ggcccggtga cttttattat attcgtagac ttcactggac
     8941 agtccaaggt gatatctata atcatcaata tcaaatccat catttcaatc agcaaatcat
     9001 gaaaatggat aaagcaacac ttttttcagg agattactat gtattggatg tctcaaatga
     9061 tgaagatgct ccagtttgta tttgtattgc tgcggtttta gattactggc tctaccgtaa
     9121 agacaaaagt cgcgataagc ctgtttctta ccgacttaac ttaagttaaa aagatgtacc
     9181 tgaaatgcgg aaatacgcat ttcaggtaca tctttttaga tattcttttg catatgatca
     9241 taaagatgac cactgatcat ccgctgaccg acaactttga agtcatggcg ttcgtataat
     9301 tttttagcct ttggattttt ttcatccaca cttagaccga tgacggagcg attagctttc
     9361 ttcgccaatt taggtaatgc ttctaataat cgcgaaccga tgccttgtcc tctaaaatct
     9421 tctgatacac tgatagagtc caaataccat tcgttgggaa acgtttcttt atcaatgaac
     9481 aaacgtgtag acgcatctag ctgatatttc tctaagatag gtaccagtgg ctgatcgatg
     9541 attgattctt cttcatctgt ataaccgtaa gcaactcctg caactttacc gtctatttct
     9601 tctacgatac ctcgtgaata actgtaacga taatttggat cagtgattgc ttcttctaaa
     9661 atctctaaag tcttttgttc ccctgtctgc ttcaaaaatg gcagttccat atcttttaga
     9721 atcactaaga tcaatggggc gatttctgga gcatcttctt ttctcgcaaa acgaatcata
     9781 gtttcctctc cttttctcca ttctaacatg cagaaaaaga aatcgcaaaa tcgttatttt
     9841 aaaaaatctt caatcttttc agtaatgaaa taaccatgag taacgattct tgccgcttca
     9901 gttcggcaat aagggcagac gactggcatt tctttatcat tatgttcgac tacattattg
     9961 acttcttcgt atgcaaattt ttttccgcaa ttagggcatt tcattttttc catgttatct
    10021 caacttcctt tcatctttac tctcttagct tactcttata ttgtaaaaaa gagaagcaat
    10081 atccttcttc tatgcagata tccatacctg acggtcctgt tctccatcta ttaatcggac
    10141 tttataataa ggacgttcat ctttgactag aagtgtccac tctttcactt ttgctttttt
    10201 cccgtagtag ctgactgctt ccttcatcgc tgcttgcggt tgtttgaact cttccgtttt
    10261 ttctatatca aaaagatgtt ctgcggcctt ttccgtgtca tttcgtacgg ttgttttccc
    10321 agtggatgga ttgactgcta tgacttgtcc ttcacaaaaa aactggtatt catatgttgc
    10381 attcgtagga ttctccaaat ctttttcaac tgcaaaatga atagcagata tgttatgttc
    10441 ttttgtttcg ttttttacga ttgctgctgc ttttttcaat gagatccgat attcctggtc
    10501 actaatatca tccatgtgaa aagcggcatt gagcagtccc gtcataccgg taatccctat
    10561 tacgatgagg gcagcaacac caaacatcat ttttttcatt cttcattctc ctgtttcttt
    10621 tttttaaaga atatcaagaa atgaacgaat gaaaaatcga atgaaacgaa aaaaaagtga
    10681 caattttgtt atctttattc gtgcagttct aatggcaatc cgtctggatc aaagaaaaac
    10741 gtcatttttt ttcctgtaaa ggtatcttct ctaatcggtt ctgattcgat ccctaaggac
    10801 tgcaattctg caattgtttc ttcaatatcg tctactttga aagcaagatg acgcaagcca
    10861 caagcttctg gatagtttgg tcgttgtggc gcattagcaa ttccaaaaat ctccaattca
    10921 gcacttccta gtttcagatc taatttatag tcatctcgtt ctttgcggta attttctctg
    10981 attacttcaa atcctaattt attaacataa aaatcacgtg atttctgata atctgaaaca
    11041 atgatggcta tatgatggat cgttgtaagg ttcatatgag cactcctatt cttcgtttaa
    11101 gctcggatct atattaatct ccagttccat tgtaaatgtg tcagattgaa aaaaatagct
    11161 gtttttcctt tttataagaa aaaaggcaaa aaaaacagaa agtaggtcct ccaagacact
    11221 cttcctgttc ccaatataat aaatgaaagt aagaaattat attttcaaga aaacaaacag
    11281 aaaattatca taacacaaat tatttataat tgaaagacta gttttttttc aagaaaagca
    11341 tacaatagat tgtgttagta aatacagaac acactacatt tagtgtctta aggagtcctc
    11401 atacatgaag tcatattttc agtttttagc tcatcagcta aaaggctggc cgcaacaaaa
    11461 ttattatctt ttctttttca gtcttggctg ccaggtcatg actcttgtca atagtcccat
    11521 cacttggctg tccgttatta catttatcgg taccacgctt ggtgtattgt gtattttatc
    11581 catcaatgcg gcaaaatcag tcaacggtat tttaggtatt ttgtctgcga tttgttttat
    11641 tatcgttggg ttttcagcaa agaactattt aagtatcggt gaacagattg cttatatgat
    11701 cacattggat atccccgtat tgttaagtaa agaatggaat cataatatgg cttcaaaaat
    11761 ccgaaaattc actggaaaaa gctggtttat cgcaatcatc agtactttcc ttgtctatct
    11821 tgtttcagga ttcctgattg gtcgttatac agatgatcct cggccgtggg tagatgccat
    11881 tgctttttct attagcttga ctgctggtat catcagtttc ttgcgttaca acaaccaata
    11941 ttactggtgg ttagcttcag ggcttgctca aatggtcctt tggtatattt catttacgca
    12001 tggatctgca agtttggcga tgttcgtgaa cagctctgtt tacttgctga atgatgtcct
    12061 tgcatttacc gtttcacctt ggtataacca aaaagaacgt gaacgtatgc aaaaaatgga
    12121 agaagaatac tacaacgcac aacaacacgc ataaaaaaac tgagcgtggg acaaaagtaa
    12181 agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaatcagaa gcaactcctt
    12241 cggaaataag ctgaaattca caaaaatttg aaaagcaatt ttcgtgaatt tcttcttatt
    12301 tcttggagtt aaacgcttct gtcccagcct ctatttttta tagtaattcc ttcagatact
    12361 ttcttgcctc atcattgaaa cgcagtgatt cttctaactt ctgataatat tccaacgctt
    12421 gtttctgagc ttcagtaaaa cgataatgtt ttcctttcca tttgtcgatt gtctcttgca
    12481 catttccaga ttggatatct ttcactacta gttgccattt ttcttttgag actgtacggt
    12541 ctttcttatc agcagattgt atagcttctc gttctttacg caatactttt aaaaaaccat
    12601 ttctgactat ccgaggaatg ctgtcgaaga acccttctgt aaaaccatag cgacaatatt
    12661 gcttccaaat aaatagatag atttttggtt ggatgaattg ttggtttttc gcaaaatatt
    12721 ccatctgagc agtgacttct ggtgttcgtt gacgttttgt ctttctcaac acggaaataa
    12781 atgtttcaaa ataagcatta ggatgtactg gcacttcttc atatacgatg gattgccaat
    12841 ctttacgtaa aaatgcgcga tagttgatca agaattcttc tacatccttt actaattctt
    12901 ctgcaaagtc gtcaaatagt tcttttttta tctgtcgctt ttttgactgc ccattgatgt
    12961 gttcacttat tttcttgatt tcattttcca atgcttgata atccgctatg taaatcggca
    13021 tttctacttg gcgaaattct gcgagccgct gagctaattt tatatccggt aaatatggct
    13081 gtttcatccg acgattttgg tacaagacaa aacagtattt ttgccatagt ccttcaggaa
    13141 aatcgatatt cttctgctta agccataata attcgtctaa ggccagatca acttttgtca
    13201 ttccctgatg gatcgaagct gccactgtcg gagagacatg caggtgatcg gcaaagtggt
    13261 tgatgcctag ttttaggatc tttccggttt ttggcgattt aacgatgtat tgcactttta
    13321 attctctacc acactcacaa tacaattttt gctggcttcg ttttgttcga aaaaacggat
    13381 tgatcttgaa gtcagaaaac acccaatctg ttgcagctaa ataatgttct tccataaaac
    13441 gagagctgat tagatatcgg cggtgcttat cgactagttc ccgctgtttt ttcgttaatt
    13501 ttgcatagat ctcccaacgt tgactgcttg tgtattgttt agaaattttg atgggatagc
    13561 ttttgctgat ttcttttgga aaccattctt caaagacgga ttgccattct tccacggctg
    13621 tttctttcct ttctaattac aaaaagctgg ctactaacat aacgttagca accagccagt
    13681 gtttcttgct aaattagtga gttgttccgg catttccgga aatatcttct ttattttcta
    13741 ctgctgcttc aactgcataa gtcaaagaag aagcaatgtc ttctaaagac atagaaggcg
    13801 tacccactgg acgatcgatc acttgatcgg gtgagaatgg cacgtggatg aacccacctc
    13861 gaattgccgg gaattgtgtt tcgatcaaat gaagcaaacg atacatgata tcattgcaga
    13921 caaatgttcc tgctgtataa gagatgtacg ctggtagacc atgatcttgt acattttttg
    13981 tcattgcttt gattggcaat gaggtaaagt aagcggctgg accgttttct tctaaagccg
    14041 tatcgaatgg ctgatttcct tcattatcag ggattcttgc ttctgctaga ttgattgcga
    14101 cacgttcgaa cgagactgct gaccgcccac ctgcttgtcc gacacagatc acgacatctg
    14161 gttggtgctg ttggatggct tctttgacta cttgactgct tttttcgaat acggtcggta
    14221 cttccacttt gatgatttca gcaccgctga tttcatctgg catttttttt actgcttcat
    14281 aggctggatt cactttgtcc ccaccaaatg gatcaaaacc tgttactagt actttcatct
    14341 gttgttcctc cgtccgattg atttaacata tggttatctt ttatcatacc attttccttg
    14401 aacagcgtaa ataaaggaaa caatagttta aataacataa cagatcaaac ttaatatatt
    14461 cttataaaaa aatagtgttc cactacaatg ttagaagagg ttgcgacaat tattctgtca
    14521 caacccctct cttctattat ttaaatagaa tcatataagc aatttggaaa actagcataa
    14581 ataacgcaat aaacaactgg tttttgatca ctccgtattt gtctttcatc tccagcatcg
    14641 ctacaggcac gatattgaag ttagcagcca tcggtgtcag cagggtccca caaaaaccgc
    14701 aagtcaatgc aaccattcct attaacgctg gattcgctcc atgactaaat acgaatggtg
    14761 caccgatccc aaccgtcatc acggtgatgg ccgcaaatgc attccccatg attaccgtaa
    14821 atatcaccat gccaagagcg taaatgatga tgccgatgtt cacatttcct tctggaacga
    14881 tcgtacctac tgcattggaa atcactgtcc caactccagc gcttgtaaat accgcaccta
    14941 gagaagctaa aagcattggc aacatgctta acggccctac tgtgcccagc atatctgctg
    15001 catcatctaa aaatgtactt ggtcgattat ctcgagaaaa gaacatcaag attagcatgg
    15061 ctgcaaaaac accgacgccc acgccgacta aagcacctaa attggtaaat agagcaaaca
    15121 agatcgcaaa gacaccgata ctcaaagctg gaataaaaat tttcattcct agtttgtctg
    15181 acattttttc catatattct tttgatggaa gccggctttc tcctttgctg actttcttag
    15241 caattgctgg tatggtcatc gcaaagatca agccaccgtt cacgattgcc ggaagccatc
    15301 ttcctcctgc aataactacc cctaagaccg tccagaaaaa ggctgaccca taacgatgag
    15361 gattggtttt atctgttaaa tttcttactc cagtgtaaat caatacgagt cccatcacga
    15421 tataaaggat ctccagtaat ttatcgccta ataaaatatc aggatttgta aaaaagtcca
    15481 tcttattgcc cctttcctgg ataatatttc ttcgctaatt tcttttcttt aagatagaag
    15541 tagacacttg ccacaactaa agccgtgata gccactggaa cttccactaa agcaagctgt
    15601 cccaatgtta cgtcatatcc tagatctttc agtgttgatt ggactagcaa cgcccctgct
    15661 cctccgatga atagtacttg gccaaagaac cagcagacat tttccatagc tgaagccata
    15721 ccttttagtt cttcttcatg cttaggatgg acttctaacc cttttgattc gatggtcccc
    15781 aatgccatcg gtaaaatgat tggacgaaca aatccagcaa ctccaccaaa actcacgtta
    15841 aaagccgcaa agatcattcg aaagatccca taaatatcta caatcatacc agcagatact
    15901 ttcttgaatc gcttgatcaa ggaagccgct gattctttca gaccatttct ttcaagcgtt
    15961 cctgttgcca acataatgat gataaaaatc gccatcccgc gattgtttac aaaacttgta
    16021 cctaaaagtt ctaacattcc ttgaatgcca agaccgccag tcaaagctgt aacaactaaa
    16081 gcaaccatta cgatcaatat cgagtctaat ttaaatgcaa acccgagaat aacaatcaag
    16141 acgcctaaaa ggctaagata attttccata tattttcctc cataaaatga caatattttt
    16201 ttaattataa agcaaaacac ataaaaaata tagttgtttt ctcacgaaat agacactatt
    16261 tttaaatatt tttcgttatt tttttattat ataattcacc ttacttattg taagataaag
    16321 ct
//
LOCUS       DAZUMK010000054        16053 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000054 DAZUMK010000000
VERSION     DAZUMK010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16053
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.055"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..424)
                     /locus_tag="ACG808_001634"
     CDS             complement(<1..424)
                     /locus_tag="ACG808_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFS5047262.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQ"
     gene            complement(554..1264)
                     /gene="budA"
                     /locus_tag="ACG808_001635"
     CDS             complement(554..1264)
                     /gene="budA"
                     /locus_tag="ACG808_001635"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFS5047263.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1277..2941)
                     /gene="alsS"
                     /locus_tag="ACG808_001636"
     CDS             complement(1277..2941)
                     /gene="alsS"
                     /locus_tag="ACG808_001636"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFS5047264.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3146..3808)
                     /locus_tag="ACG808_001637"
     CDS             complement(3146..3808)
                     /locus_tag="ACG808_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS5047265.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(3818..4633)
                     /locus_tag="ACG808_001638"
     CDS             complement(3818..4633)
                     /locus_tag="ACG808_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5047266.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(4895..5338)
                     /locus_tag="ACG808_001639"
     CDS             complement(4895..5338)
                     /locus_tag="ACG808_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HFS5047267.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(5472..5810)
                     /locus_tag="ACG808_001640"
     CDS             complement(5472..5810)
                     /locus_tag="ACG808_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS5047268.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(5798..6175)
                     /locus_tag="ACG808_001641"
     CDS             complement(5798..6175)
                     /locus_tag="ACG808_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS5047269.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            6399..7592
                     /locus_tag="ACG808_001642"
     CDS             6399..7592
                     /locus_tag="ACG808_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5047270.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(7758..8180)
                     /locus_tag="ACG808_001643"
     CDS             complement(7758..8180)
                     /locus_tag="ACG808_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5047271.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            8551..9225
                     /locus_tag="ACG808_001644"
     CDS             8551..9225
                     /locus_tag="ACG808_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047272.1"
                     /translation="MKKLVALFLVAGSLLSGCGATTSTTQSTSSAQASSSSTTISEST
                     ADSSKEQEQIEKKKEEEKQKAEKAALEKKVTEAESALSQAEANPTKENYATASAAIQA
                     LPENKQELTSRLAAIDSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQ
                     AAAEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPC
                     SKCYGN"
     gene            complement(9387..10559)
                     /locus_tag="ACG808_001645"
     CDS             complement(9387..10559)
                     /locus_tag="ACG808_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS5047273.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(10765..12063)
                     /locus_tag="ACG808_001646"
     CDS             complement(10765..12063)
                     /locus_tag="ACG808_001646"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS5047274.1"
                     /translation="MHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYP
                     SKDTAVPADATPFASVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLS
                     LPFYNPNSSDPDKPLDTIHLYAKNVIKESNIKIQKAGNFSGVDKLAGAQFQIKNSENQ
                     FVTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVETKAPDGYQLTNPQ
                     KETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTITVENVQNYGDFHF
                     IKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWSDEVTGSDWEEVKL
                     ISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDDDPTTSDETTIDNQ
                     PKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(12191..12706)
                     /locus_tag="ACG808_001647"
     CDS             complement(12191..12706)
                     /locus_tag="ACG808_001647"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFS5047275.1"
                     /translation="MDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFT
                     SSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEP
                     KANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKK
                     QQKGNNRREIK"
     gene            complement(12779..13693)
                     /locus_tag="ACG808_001648"
                     /pseudo
     CDS             complement(12779..13693)
                     /locus_tag="ACG808_001648"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            complement(13690..16017)
                     /locus_tag="ACG808_001649"
     CDS             complement(13690..16017)
                     /locus_tag="ACG808_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HFS5047276.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
BASE COUNT         4571 a         3222 c         2851 g         5409 t
ORIGIN      
        1 cttgatcgat ttcatcatac cctttcgtta ctccgcaatc gatatctaaa acaccgatta
       61 aattaccttc ttttaccatt ggcactacga tttctgattg tgcggctgaa tcgcaggaaa
      121 tgtaatttgc atgggttttg acatcatcaa cgatcaatgt ttcttgtttt tcagctgatt
      181 ctccacaaac tccttttccc atcttgatac gtgtgcaaga aactcgccct tggaacggtc
      241 ctaaaatcaa ttcattacca tcgaataaat aatatcctgc aaagacagtc tcaggcaaag
      301 tctcattcaa caatgcagat gaatttgcta aatttgctaa ccagttatct tctgcttcta
      361 ataatccttg ttgctgtaat aataaaattt cgtatgcatc ttttttctgt ttttctgtcc
      421 acatgataaa aaccgctcct ttttgatcta tggagaagat tatagctcag tttttttgta
      481 aacgctaggt tttctcctca aaaaaacgag atacgtcagg ttaatcacct tgaacgcatc
      541 tcgctgtctt tttctattct gcttgttgga tttctgctgc taaatcagca taatcaatcg
      601 tttcgtgaac aaaagtcgga tcatcaactg gaaaatgctg tacaagggac gagatgcggc
      661 taatctcaac ttttccttca gccacttcaa aatccattac atgtccgcca aatgtatgtg
      721 tatcatcgat aaaatgcagg tgaaatcccg ctgcagcaac tccttggaat aattcaggcg
      781 taaagaaccc gactaatgtt ccttggacat tgctttgttg gaattctggt tgagactctg
      841 aaatttttgc tagtcttctg taaggttttt cttgcttcgg catgatacgg atgtgcatat
      901 ttttgaataa tccagttatc ttcactgcca aaaataggtt ttccccggct tctcttacta
      961 atatgtcttt tttcaattgt tctccatctg cagtttttga gacagaaaaa ctagtatctg
     1021 gcgtaaaatc tgtaattgct gcataggggg taagttcact tccatctaat tgggcaactg
     1081 agccatctga tcttccttga taagctacgc catctaaaaa gatgacttca ccatcaagcc
     1141 catgcaatgt tcccaatccc agagttcctt tttctaatat atgagcaatc gtttctgttc
     1201 catccatcaa gccagccatc aaagctccta atgtcccgtg ctggtataag attttttcag
     1261 tcatgtcgac acttccttaa tagaattgat ctggtaagat tgtttctccc aatttctcat
     1321 tatcacggta atcaatcgga atatcaataa ttacgggtcc ttctgtcgca aatccttctt
     1381 ctaaagcttt ttctaattcc tctacacttg tagctcggat tcctttagcg ccaaaagctt
     1441 ctgcatattt cacaaaatca actggaccaa agtctacacc tgacgcccgc tgatatttca
     1501 ttttttcttg gaactctacc atattgtagt gtccatcatt ccaaatcaaa tgaacgatat
     1561 tcaatttttt acgtaccgcc gtttctaaat cctgggcaga gaataaaaat cccccatcgc
     1621 cggaaactga tacgatttgt gtatttggac gcaccaatgc agcagaaatc gcccatggca
     1681 atgcaacacc aagcgtttgc ataccattac tgaataacaa atgacgaggt tcataacttc
     1741 taaagtggcg tgccatccaa atataatgac tacctacatc tacggtaaca gtcatatcat
     1801 ctgttacttt tgactgaagc gtgttaataa cttctaaagg atgcaagata cctgcttctc
     1861 cttttgattg aacaatgtcc cgctccgtta attctgcttg aagagatgct aaatattctt
     1921 ttgcatcttc tgacacttgt tgaggctcta atgaacctgt caataaatca agagtggctg
     1981 agatatcacc gatcaattct ctttcaggct gcataaaagg atcgatttca gctggcgctt
     2041 catcgatcac aatgatccgc gcatcttttt ccgcattcca gtttcttgct tcatattcga
     2101 ttggatcata gccaatagca ataactagat cacttcgttt caaaagcata tctcctggtt
     2161 ggtttctaaa taaacccaca cgaccaaaga aatgcgcttc taattcacgt gagatcactc
     2221 cagctgcttg gaatgtctct acaacaggca atgctgtctt tccaactaat tgcctgatgg
     2281 ctaacgtttc tttttcactt gatcctcgca tacctactaa tagtaccggt agttttgctt
     2341 ctctaatgcg ttcagctagg taacggatat catctgcaga agcagaacct aatttaggat
     2401 cgctcaatgg cttgatgaca ttcccttgga caggtgcatc cactacatct tgcgggatac
     2461 tgataaagct cgcgcctttt tttgaactct tagccatacg ataagcattg gcaatcactt
     2521 ctgaaattgt ttctggatct tggatttctg cactgtattt tgtgattggc tggaacagtg
     2581 ctgcattatc catgctttga tgggtcaact tcaatagatc gcttcgtttt acttgtccgg
     2641 caattgctaa aactgggtca ccttctgctg tcgctgttac caaacctgta gctaagttac
     2701 tagcaccagg accacttgtt gcgataacta ctcctggctc tcctgtaata cgtccgattg
     2761 cttgtgccat aaatgcagca ttttgttcgt gacgagtcac gattaattca ggaccttgat
     2821 cctctaattc attaaataca ccatcgattt ttgcacctgg tataccgaat acataatcaa
     2881 catgatgatt aatcaaactt tctactacag atttcgaacc ttgtttcatt ttttcgtcca
     2941 ttgaacatcg tcctttttat gctttatttt taattattag ttagaaataa tcacaaataa
     3001 ttgatacagt ttctaaacct gtttcattca aaagtatagc acgttttatt tcatttgttt
     3061 agccatatgt ttcaaaaaat gaaaaagaat gacagttttt acataaaaac tgtcattctt
     3121 aattgaaagt ttcaatttat aaagtctaca gatccatagt tctcatcaat ttacgattgt
     3181 tgaagtaaat caaaccaaat acgatagaag gacctagtag aatacaccat tggggcttta
     3241 cgaaaaagaa gagaaacaaa cttgtcaata gtaaaaaatc tagtaaactc acataccatt
     3301 ttttcaacgt atagtagaac gctatccgta gaacttgatt tggtttcgac gctggatttc
     3361 tcacctgaaa ataccagtag ttgatgctaa aggaaactga taaacaagca agtaaaacaa
     3421 acaacggaat caaccatttt ccataagaaa aacgtataaa aaagaaacaa tcaaaaatca
     3481 ttatcacact aactatccaa gcaaatagcc aataaaaaaa tccctttagc caaaattggc
     3541 gaaacgtctt tatgaacaaa cgtactggtg aaaaactttt ttcttttgct aattcttctg
     3601 tcatacctat catcacgatg caacctggac caataaaaag taaagaaaaa ataaaggaaa
     3661 agacattcct tccatctatc gacaaaagga acgcagctgc tatatacaag atattgacac
     3721 accaaaaaca aaggctactt gccaatgatt gatacaacca ataaaagaat tccatataaa
     3781 catttttgtc aaaacgctgc tctttcataa tcacctccta ttccttcatt cctgatgaag
     3841 cgatcccttc tacaaaatat ttttgcatcg ctaaaaatat aaaagcaaca ggaagaacag
     3901 aaacaactga ggcagccatg attaatgcgt actctgcatc aaatagccca ataaacattt
     3961 tcaaccctaa ctggattgtt ttattttccg tagaagacaa ataaatgaat ggccccatat
     4021 aatcgttcca tgtattaacg aatgtgatga tcgtcaaact ggcaatcgcc ggttttgtaa
     4081 gaggtaaaat gatcttgcga tagattcccc actctgataa gccatcgatc cgcgcactct
     4141 cacataaaga atctgggata ctactgtagt attgcttcaa caagaacaca ccaaaagcat
     4201 tgaacgcttg taatagcacc aacgaccaca acgtatatgt taaacccact tttctcatca
     4261 taatgaactg cgggatcata taagactgcc aagggacagc aatcgtgccg atatatgcta
     4321 aaaataaaat atcgcgccct ttaaaattta gtttagaaaa cccgtaagca gcaaaactag
     4381 aagtaaacaa ctgaatcaac gtgatcacaa cgcttaaaaa agctgtattc ttaaaaaatg
     4441 tcactaaagg aatacgagac caaataacca gataattctc taaatgccaa gttttaggga
     4501 tccattgaat cggaatcgtg aacacttcat tatttgcttt gaatgaagca gaaaccatcc
     4561 ataaaaaagg aatgatcgtc aataaagcta atacaatcag cagaatcata cccaaccatt
     4621 gaataaaggg catgtttttt gaacgaattt ttgctttttt tctttcattt cgaacacctc
     4681 caaagttata atcttcattt ctttgtaatg attacaatcc agaacaacgg ttattatagc
     4741 tgattcattt ccgttcttct tatgtttgta agcgcttatt taacaagtta tgtaaatctc
     4801 tttatttttt ttgatttaaa aatgaaaaaa tacaaaagca agactgtcat acgtctcgct
     4861 tttgtattga aatttcttcg attagagata cttattactg ttcaaaagct tctttgaaac
     4921 ggtgaatcaa ttcattgact acaggtgata aaatccgatt ctttttccag actagcatgc
     4981 agttcgtttc aatcattggg tcaagcggaa cgaatgtcac tcgctttgaa agatgactta
     5041 cagcaccttc tatgactaaa gcagcaccta cctgattctc aattagcgca aatacattga
     5101 aaatcaaatt gtacgttcct gtgatcagca actgctcctt ttttatgcct aaccagtcag
     5161 caaacattcg ctgtatttct ggacgctttg agatcaaaag aggtacattc gcaaagtctt
     5221 cttttgttat cacgtctttt tttgctaaaa cagaattttc taaaatgagc aatccccatt
     5281 tttcctttct tggtaacaaa atcgtttcgt atttctctgt atctactgga tctagcaaaa
     5341 tcgctaaatc taacaggctt ttttctagtc actccgtgat atcatcactt gttccgctgt
     5401 ggatatgaaa ttgaacgctg ggataatcac ggatcaactt ttctagtaat agtgccaatg
     5461 tattcgaatt atcagcttcc acacagccga tcgtaaatcg gccgctgaat aattgttttt
     5521 ttctgttctc gaattcttga gtggtttgat tcgttaaatc taaaatttct tctgctcggc
     5581 ttttttagaa aataacctgc ttctgtcaag atcattttgc ggttttcgcg tacaaacagt
     5641 tctgttccta gttcctcttc taactccttc agttgtcggc ttaaggttgg ttgtgtaaga
     5701 tgtagaactt ctgcggcttg agagattgtt ccttcttctg caattgtcca aaaatatttt
     5761 agtaagcgta actccatagc gatgctcctt gattttttta cttgatacat tccattttat
     5821 ttctagataa cacacgatca tatttcttga tttttccttg taaacgttcc aaacattctt
     5881 gcatttcagc aatttttttc gtcaaaagtt cactttcttc aatcaaaagc ttacgccgct
     5941 caaatacagt tgcttctcct tcgctagata ggcgaatata ttcaatcaaa gactcgatcg
     6001 ataatccggc atttcgcata cagatcgtaa agtcgaccca ttttaggtct tcttctgtat
     6061 aatcacggat tccgcctttt gtccgattaa ctggtggaat caacccgatt cgttcataat
     6121 agcgcaatgt atcggccgaa atgccctttt cttcactgac ttgtttgata ttcatctgat
     6181 cactctttct cttaatttct tgccacttta cttatgtatt ttatttattc tacaacttta
     6241 agtgtactcc aagtcaatac tcatttctat taaaactaag ctggattatc tatatcggtt
     6301 gtttgtgtat tatttcaaat aaaaaagtct tgttttttta ttcttttttg gatatacttt
     6361 taacaattta gggaaatttt aggaaaaggg gtataaaaat gacaacgtat cagcgaaagt
     6421 tattattatc gacatcggcg ggaattgctt tagagaatat ggatattatg tttttagctt
     6481 tttcattatc ttcaatgatt acttcttttc atattagtgg tacgcaagcg ggtttcattt
     6541 caacgatcac taatttgggg atgctcattg gaggtatttt ttttggcatc atagctgata
     6601 aatatggacg tgtgaaagta ttttctcaaa cggtccttct gttttcgatt gcttctttat
     6661 tgatgtattt tgcttcaaac atctacttgg tttatctctt tcgctttatc gcaggtgtag
     6721 gagcgggagg agaatacggg gcgtgtatgt ccttgatttc cgaaagtttt tcaaaaaaac
     6781 aaattggacg agcttcttcg gttgctggta ttggtgcgca ggtcggtgcg gctttggctg
     6841 cgattttagc tgctgtgatc attccaacgt ttggttggaa gatgttgtat gtcgtaggtg
     6901 tactgccggt gttgatggtc ttgttcattc gtcgcggctt gactgaacca aaggaattca
     6961 aatctgtaca atctgccgaa aagaaaacca aacttagcag actgtttgaa tcaaaacatc
     7021 ttgcttggca aacatgtggc cttagtatca tggtaactgt ccaaattgcc ggctattttg
     7081 ggttaatgaa ttggctgcct tcaattatgc aaaatcgatt agggctttcg gtttctgggt
     7141 cttcattgtg gatggtcagt actattttag gcatgtcttt ggggatgatg acttttggca
     7201 taatcatgga taaattgggg cctaaactag ccttttcttt gtttttaatt tgttcatcct
     7261 tatctgtcta tctacttgtg ttagcccata gccagtggag tttggtagca gccgctgtga
     7321 tagtcggcta ctttatcaat ggcatgtatg gtggatatgg cgcagtgatt agtagtttgt
     7381 atccaacaga aatccgcgcg acggcgaata atttcattat gaaccttgga cgagctgttg
     7441 gtggtttttc ttctatcgtc atcggtttct tgatggatca ttatagttta actgccgtaa
     7501 tcttgttttt gagcggtatt tacctagtca gtctgctggt cttgacaact cttactggtg
     7561 ttacagcatt aagggaaagc ttgcagcatt gatagttgag tctgtaaatg acaagaaaga
     7621 ggtgtggcaa aagcttgcat ctgcggtaat aagaccagcc aaatcaaaaa catgagacac
     7681 tgtttccgtt tggctggtcg tattacttga tgcagggcag ctttttcaac tgctaatttc
     7741 cttctatctg gcggctacta ttctttgacg tccgtcattt caaagtactt tactaataac
     7801 ttagcataac gaaaaactgt ggatacactc gtatattgag cgtctgcata atcttccaaa
     7861 tttttctcag gatgttgata catgaattca atcaaatgat aattatatga ttttctgtag
     7921 aaatgaagat aaataatgta gattgggaag ttctcctgct tttccataga aaagcgccga
     7981 ttattctcat tcttcaaaat gaccttatca ggatattttt ggaacaattc ttctaaatct
     8041 gttagataat tcaagataga cttacttgat accttcaatt cgttttgtag ttcttctatt
     8101 gaacaccaac gttctgtttc aagcagtaaa tgaatcattt taattttctt ttgagaaact
     8161 tttgttaaaa aagtctgcat attttctcct cctgtatcta ctttactata ctacatacaa
     8221 gaaaaaaaac ggatacaaat atcatattgt cacaccgtca atagtgcata aacctgttat
     8281 tatttctctt tttttcctaa gtattctatt gatatgaatc atttaatcgt ttattatata
     8341 aatgtcaata atctaataat aaaataacgt acatatttta ttattgcata cttaataaca
     8401 gaactatagc aaaaaatccc gtgtatcaaa acgggattag attaagtttc ctcaatcact
     8461 attcataaca caattattat aacaagtaag taaacaattg aaaaggacat ttattgaaaa
     8521 cggttgtttt ttacttaagg aggaaattta gtgaaaaaat tagttgcatt gtttttggtc
     8581 gcaggaagtt tgttgagtgg gtgtggtgct actacgtcta ctactcaaag cacaagttca
     8641 gctcaggctt ccagcagcag taccacgatc tccgaaagta cggcagattc atcgaaagaa
     8701 caagaacaaa ttgaaaagaa aaaagaagaa gagaaacaaa aagcggaaaa agctgcacta
     8761 gaaaaaaaag tgacagaagc ggaatctgct ttgtcacaag ctgaagcaaa tcctacaaaa
     8821 gaaaactatg caactgcttc agcagctatc caagcgctac ctgaaaacaa acaagagttg
     8881 acaagccgtc ttgctgcaat tgattccaca atcaaagcaa aagaagcaga agaagaacgg
     8941 aaaaagcaag aagaaattgc acgacaacaa gcagaacaag cgcaacaaga agcaatcgca
     9001 cagcaacaag cagaacaggc gcgacagcaa gcagcagctg aacaggcctc tcagcaggca
     9061 caagccgcag ctcctgagca aaataatgaa caaactgtct atgtcacacc gaccggttca
     9121 aaataccata cacataaatg tggaaatggt acgtataccc ctgccacttt aagcgaagct
     9181 caagctcgtg gtttaacccc ttgctccaaa tgttatggaa actaattcaa aagttatact
     9241 ataataataa aattcttctg aaacataaaa aaatagaagc tggcgttcca gatagtaaac
     9301 caaaaatagc agtttacacg tccatttcgt cagcttctct cttttttcat ttagtctgtt
     9361 ccctctgctt ttttggacat cttcattcat tctttctcaa tcaaatagta cacctctttt
     9421 tcttttcgca gtttatatct tccttttttc aatttcattg aaaactgttt cgaatgttta
     9481 tgttttacat ggattttaag caggagctcc tcatgttgca agcgatagtt cctgcctttt
     9541 aaatcagaaa atctcactag tccgtattca tcagaagctt tcacaaatgg aactttttct
     9601 ctagtaatat agtgtttgcc gttgagcgtt tttacagaaa agaccattcc ttcgattggt
     9661 cgtccgtttg tgttctttac ataaaaagat aaaggatagt ttcgcttagc gatggccaaa
     9721 gaatctaaat aagcgatgag tagccaaact aagccgattg atccaagtag tataccgata
     9781 aaccaaataa aaaatttcca ttttttccat tgatcaattc cttggatcgc tttttttgcc
     9841 tcttttgctt gataaggaat acgatgcccc gtcacaagca gtcgatgtgt attgaccata
     9901 taaggagtac aggtaagcaa agtcacgaga tctctgcctt tttggatacc caaagaatct
     9961 acttcatcag gtaagaccac ttggattttt tctacttgat acgccaatgt ctttccattg
    10021 atctggatga aaaactggtc tccttttttc aacttaggca aatctgtaaa taattttgct
    10081 tctgggagtc cacgatgccc agaaagaacg gcatgggtgt ttttcccgcc agtcggataa
    10141 gaactttctt ccaataatga cgttcctttt tgcaaaaata tttccgttgt ctgatcgaat
    10201 ataggtaagc tgacattgat ttttgggatt tcgatgactc caagcatatg ttccataaaa
    10261 aaggcattac taggtaaatc ttttaatgct tttgcatcaa ctgcttggtt gaaactgcct
    10321 atccctggtg atacgttttg ctctgccaac tgttggtttt tctttgtcat tttttcctgc
    10381 tgttttttta tttcctctga attcttattc gatgcttgct tttgataatg gatgatcatt
    10441 tgctgggcaa gaaaatcatt cagtgaatct tctacaaaag gataagaaaa gatcccgatc
    10501 ccaacgacta aaatgattgc catgaagatg tccagtattc ttattctgct tttattcatc
    10561 atgtccacct attttctaat atgtatatga agaggttgtg aaatcagcgc cttgacctga
    10621 gtattcgctc aacaaacggc aaaatagttc ctcctatttt gccgtttgtt gagcgaatac
    10681 caagagaccg accacttgtc acaacctttc tccccatttg aatcactatc gtttatttat
    10741 cgtatgattt ttgtttccaa tagtttaagc ttctgtttct tgatggcgtt ttttgaagta
    10801 gatcactgct cctccaacta aagcagtacc taccaagatg aatacgatga tccccatacc
    10861 accagttgaa ggcaagattc ctttaggttg gttatcgatc gttgtttcat cagaagttgt
    10921 tggatcatca tctactgtaa attcagtgta cgcagcttca ttcttaggta atgcgtagcc
    10981 ttctggtgct tttgtttctt gaaggcagta tgccccttgt ttcagccctt cgattgaaaa
    11041 tttcccttct gaatcagaaa tcaatttcac ttcttcccag tcagaacctg tgacttcatc
    11101 tgaccaagca tatgggtatt ctcctgaggc aagatttgct atttttttca ttaatttaac
    11161 agttggatct tttgctctta gaatagaaaa ttctgcatcg gctaattttt cttgcgtgtt
    11221 cacatcttgt ttgatgaagt ggaaatcacc gtagttttgg acattttcta cagtaatcgt
    11281 gttatccgta gttgtatttt cccgattgac tacaccgttt ggattcccaa gattggcttc
    11341 atctgcgcca tggatgatac cggttgtttg ttgcaatttt ccgtttttta ctataaaagt
    11401 agtttctttt tgtggattgg ttaattgata accatctggt gcttttgttt caactaaagt
    11461 ataattgccg tctattaatc cattgacact gatggatcct tcagtatcag ttgtgaatgt
    11521 caacgctgac tcagcaggat catagagagc gacaccagtg ttttcatcaa aaccggtcac
    11581 gaattgattc tctgaatttt tgatctggaa ttgcgcccct gctaacttat ctacccctga
    11641 aaagttccca gctttctgga ttttgatatt gctttctttg atgacatttt tagcatatag
    11701 gtgaatcgtg tctaaaggtt tgtcagggtc tgatgaattc gggttataaa agggtaagct
    11761 taacacgata tttggcgacg tttcgacacc gggctttggt gtttctacaa agacgtatac
    11821 agcatctttc ccatcagatt tacctggaag agtcaggtta gctaatcctt caccagaagt
    11881 cgttacactt gcgaagggtg tagcgtctgc tggaactgct gtgtctttac ttggatagta
    11941 cgtagcagtg atgtcatata cgttgaattc aacgccgttc aacggttcgc tattcggaaa
    12001 tgtcaattcc gttccgttgt ttatttgatt ttctggtgct gaagtgaatt tcaatttatg
    12061 caaattaacc gtaacatttt ctggtgtagg tgatacggcc tagaaacctc tgcagttcct
    12121 aggccgagaa cgaaaggaag cagcatgata gcagttccta aaaacgtcca tatttttgtt
    12181 ttgatgtttt tcattttatt tccctcctat tgtttccttt ttgttgtttt ttataaaacc
    12241 aagctgtccc tgttgaaagg actagcaaca accctaaaat tgagaaacct ccaagcatct
    12301 cttcgcttgt ttttggaaag cttcctccat ttttttcatt tgatggacca tttgtctgca
    12361 tctgaactgg cgtttttcca ttattcgtcg gatttgcttt tggttcgttg atgatttcca
    12421 atggtatagg atgaagcgga tcataactgc ctttttcaac ttcaaagaga atggttgatt
    12481 gactcaatat atatccttta ggcggtttaa tctccactag ttcgtaactt ccaaaagaca
    12541 atccatggac agaaaatgtt ccatctttac tggaagtaaa aatcgttgct cttgaatcat
    12601 ctttggctac ccaatgattt atcccgtttt cttgcgctaa gtattggtca ttttttttga
    12661 ctagaaattg agcatctgct agtttttgtt tcgtttgccg atccactttg acaaattgtt
    12721 tgcctcctgt ttggatggca agatgtgttt ctactgtttg attgtcagtc gtcaaacgta
    12781 catcgttatc aaatgtcgtt ttttcactgt tttctttcaa tgtcatttgg taacggatcg
    12841 ttaatttttt cccagcaaac gaactaagat cttttccaga atctgataag acaaaaccat
    12901 gttttgtttt ttgtgtgtga aatgtatgaa tcttgtctcc accaatcaaa aaatcgatcg
    12961 tttctggttc tagccataac tgaggatcag ctgtatcttt gatcgtatag gttcctatct
    13021 cgttgatgtt ctcaggaatt gttacggtga tgagataagg aactttatcc ccatctgcaa
    13081 aatctgtctg cttattctct actgtttttg ttaatgtgag tgcagcaggt attttttgtt
    13141 cgctctttgg atacaagtgg atggtcgtca atttatgatt agatgaatct gtcacaggaa
    13201 gcacaacgac aaatggtgct gatttttctt ttacttaatc tgaagtcttt atttccgcaa
    13261 atcggtaaac agcgtcacga ccttgctcat ctttgtccga tgcactaaaa gaagcaatgc
    13321 cctcttcctc gtctactgtt tttgtcacat tttctgccag tattttttca ctatcacttt
    13381 tttgtgctag ttttctttgt gcgtcttcca cactacttcc ttctgaccgc agcttgtaaa
    13441 attcatccga gacatcatat atagcgaaag taacgccgtt caaccctttt actccttgaa
    13501 agttatccgg gatatttatt ccgtcgtttt gactgatact tggcatctta tcgctttcga
    13561 acattcgatt atgcaaagta aatgtaatag gtgattctct agctgacaca gtcggagaac
    13621 ctataaaaaa gaccagtagg ataaaaaaga gaacagcaaa tatatccaca cgtacatttt
    13681 ttgttttttt catcgctgtt cacctgcttt tttcttacga tggataaaat aaaagaagcc
    13741 agaaaaaata aagatcagtc ctaaaaagac aaagggcagt agacctatcc cacctgtcga
    13801 aggaagcaag agagtcggat tgatcgtcaa cttagcggta tctgttgcga taatcgtata
    13861 attattgttt acacttcctt cgcctaaact aatcgttact atttgtgatc caatatctgt
    13921 ccggctgtta actgttaggg tataatccaa tgattcgcct aagacaacac cggtaacgtg
    13981 gagatttcca accgaaatcg agtgactttg ggcatcataa ttaaaggcag cgtcatcgac
    14041 agtaatcact gcgttagctt tatagatggt ataagttcct gatgtgttgg ctatatcagt
    14101 aaatgacaac ttgggatttg ctgctttgac tttgtctatt ccgctattcg ttagtttaat
    14161 tgtatagctg ccagcattaa ccatattttt tgctgattgt gcatctgctg tgtaatccat
    14221 actgctaagt gccacttgta ctggagtaag cgaactagtg acaccctctg ctagcttgac
    14281 atggattgac gggacacctg cttttccatc atatgtctta ctgccactga gctctaccgc
    14341 accgctgatt tgcttaatcg tatagacatc agatgtctgc acgatagtaa ataaatagtt
    14401 tgttctggca gctagtttag ctagtccgct ttttgtcaat tgccaagacg tataggttcc
    14461 aatttcagta ggcgcagtta cttcaactcc cttttcattc atcgctttga aatcagttgg
    14521 attccatgtg atttgatcaa tactgagatc attgtttaaa agggatgttg attctgtttt
    14581 atcattaaag gcatatttaa cgatcggtac aacagaaaga ccatcatagt acttactgcc
    14641 atttgcattg attgatactg ttgcatttgt cttataatat aggatgactt cagaaatctg
    14701 ttttccattt gcgtccttta cgattacatt tccatttgta tctaaactag ctaaaacact
    14761 cattgctgat tggttctttg tttctgtcgc tgaaacagct gtaaaggtat atccgttgat
    14821 agtaggcgta ttagtcgtca ggtcttgtgc agcacttccg taagtaagat tcattatttt
    14881 agaaggagca atcatctgtc caaaatagtc ttgatactta atagtgattg gtgtttgttt
    14941 gcctgtatag gtcaccgtat cgacgttact ttctagggct gtgaaagaac cgtttgtttg
    15001 tgcaaaagaa tagcccgtta ctgctgttgg agtgataacc actttgtcac ccacatggat
    15061 tccagataat gtagtcccgt acgttccgat ttttgctgct ccaccattga ctgtataaga
    15121 aaaagttcct gtccgattcg cattgacctg aaccgttgct gcttgatagg ttggaaaatt
    15181 tgaaacattt cccttaccat caacgatctg cccatttttg atcgagtacc ccgtgaatgg
    15241 aatatcattt gctaactgta taccgattga aaaagtactc atacctcctc cggcaggaag
    15301 ccctccccat gttgagggtg taaaagaata acttttatcc gcattgggtg taactgaatc
    15361 gatcgtattt ccagcggtag ccacccccat atagatactt tttgggaatg tgatagtagt
    15421 accatttact gtggagccaa taaacagatt atggatatct ggacggttat caataaaagc
    15481 gattgctttg gttacgcctt gtaacgcagt tttatccgca ctcatcgatc cgttaaccgc
    15541 aacaatgact ccatctttgg catatatcac tgtatatcca tctggtatgg tatgtccgtt
    15601 gaaattcgca gtggccgcaa cagtactaaa tgtctggctt ctacctgttt ctgaataagc
    15661 tttctcccct gctgcttttg ctgctgtgat ttctttttct gtcggatcaa tcagttgagt
    15721 cgaggatgtg gttgaggttg tctcgttgtt gctagactga ttggtgcttt ctatggactg
    15781 tatttcagaa tagggtggac tatttgtctc ctctgtcgtt tggttagcaa aatttgacgt
    15841 ttctgtataa acgtttacat aaatacttga taagctccct aagaaagtaa aaatcaagat
    15901 gatcgaaatc ataatgggat gtaagtattt tcttttagat acagctttga gtctagaaac
    15961 aaaatgtttt tgacatatac gttggatttt attttgagtt ttcttaatca ttttcatccc
    16021 tcctctcaat aaaaaatcat caaataatcc ttt
//
LOCUS       DAZUMK010000055        16039 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000055 DAZUMK010000000
VERSION     DAZUMK010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.056"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(541..726)
                     /locus_tag="ACG808_001650"
     CDS             complement(541..726)
                     /locus_tag="ACG808_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047277.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(1576..1917)
                     /locus_tag="ACG808_001651"
     CDS             complement(1576..1917)
                     /locus_tag="ACG808_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS5047278.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1917..2147)
                     /locus_tag="ACG808_001652"
     CDS             complement(1917..2147)
                     /locus_tag="ACG808_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS5047279.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            2434..2772
                     /locus_tag="ACG808_001653"
     CDS             2434..2772
                     /locus_tag="ACG808_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047280.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2977..3539)
                     /locus_tag="ACG808_001654"
                     /pseudo
     CDS             complement(2977..3539)
                     /locus_tag="ACG808_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(4271..4852)
                     /locus_tag="ACG808_001655"
     CDS             complement(4271..4852)
                     /locus_tag="ACG808_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5047281.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(5035..5661)
                     /locus_tag="ACG808_001656"
     CDS             complement(5035..5661)
                     /locus_tag="ACG808_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFS5047282.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(5683..7014)
                     /locus_tag="ACG808_001657"
     CDS             complement(5683..7014)
                     /locus_tag="ACG808_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFS5047283.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(7169..7570)
                     /locus_tag="ACG808_001658"
     CDS             complement(7169..7570)
                     /locus_tag="ACG808_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFS5047284.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(7656..7862)
                     /locus_tag="ACG808_001659"
     CDS             complement(7656..7862)
                     /locus_tag="ACG808_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047285.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(7957..9459)
                     /locus_tag="ACG808_001660"
     CDS             complement(7957..9459)
                     /locus_tag="ACG808_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFS5047286.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(9477..9932)
                     /gene="argR"
                     /locus_tag="ACG808_001661"
     CDS             complement(9477..9932)
                     /gene="argR"
                     /locus_tag="ACG808_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFS5047287.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(10030..10647)
                     /locus_tag="ACG808_001662"
     CDS             complement(10030..10647)
                     /locus_tag="ACG808_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFS5047288.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            10868..12241
                     /locus_tag="ACG808_001663"
     CDS             10868..12241
                     /locus_tag="ACG808_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5047289.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            12316..13428
                     /locus_tag="ACG808_001664"
     CDS             12316..13428
                     /locus_tag="ACG808_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS5047290.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(13500..15137)
                     /locus_tag="ACG808_001665"
     CDS             complement(13500..15137)
                     /locus_tag="ACG808_001665"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFS5047291.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(15305..15913)
                     /locus_tag="ACG808_001666"
     CDS             complement(15305..15913)
                     /locus_tag="ACG808_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS5047292.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         4479 a         3287 c         2767 g         5506 t
ORIGIN      
        1 atgcttttta tagaatagaa ctagtgttgt tttcatagac tctcctttct tcatatttcc
       61 aattaatcac aaattcacgg cattactaga aaaattaaag gggcgcagaa gtggaaaaat
      121 actcttcgac tttccctaca aaccaggcca tcttgtgttt aataactata ccaaatctaa
      181 ggtacctcta aatctaaatt ccatttgtgg cggtgacctc caatttttaa ctcttctatc
      241 tcccatccag tatacataac cttgatactt cgaccactta tatatcgaac acacaaatac
      301 ctatccattg caggagtact acctaactta gttgctactt gcatctaaat aacagctgaa
      361 aagataggac ttactcttag taaagtatct gaactattaa tttttaaact atcattttta
      421 tcctcaacta catgcacatc tgttgcaact tcactttctg tctcatccag ataaatgatt
      481 aaggaatcgg gaattgcaat atcttcagct gacgctggaa tagttgagct aaattagtca
      541 tcatatcttt attataaaat aaccaacagc aaaaaataca atgaaaaaca aacttggaac
      601 taatgtatca ttaataaatg ttgaaaaatt cagcaactca ttactgtatg attttagcag
      661 cccaaccaac atatacccta taaaagctaa taagatggtt aggaatcctt tagaattttt
      721 actcattcat agacacttcc ttaaataact tttattatag aaggtattgt aaataataat
      781 attgacaaaa agtgactcaa aataaaaaaa aacttacatt atactggaat gaactgtatt
      841 gatagtgtac ctaaaagctc tactgacata agatcgtgta atcggtaaag taattttatt
      901 aattatacgg gatgtaatca attagagaat aaactaatca taaaaggtac ttttacattc
      961 ataattgtcg ctttgagatt ctactttggt tcactatctg acccaattgc acattttcga
     1021 tcattacaag tattggtgtc gttagaacaa ctaaataaaa actagcggta gatttttcag
     1081 aatctatgct aatttctgta tggatgttcc agtgatggca aatttctctt ttcattgttt
     1141 ttcttgtcgc atgatatgcc tttttgtgtt attcagttaa actatagaaa ttctttccta
     1201 gacagatctt gatcccaaat tttacgtttt tgagcatcga tatagtttaa aatgacatta
     1261 ctcatatcta aagagtttag attttttagg actaaaactg tcagttgttc tttttcttct
     1321 ttctgttaaa gtattgtcgc gacttttcgt atacgttaag atatgtcgtg aaaaaacaag
     1381 ttttgtgaca caaaaaaacg aatgacttga ggaaatcacc tcaatcattc gtttaaaatg
     1441 ctatatattc tttagtttaa gatacggaaa ttaatgccat cgaccacact agtagaatga
     1501 acgacattta cttggccttt ttttaatttc gttatgttta gcatataaaa atattataga
     1561 gtttacttac aatgcttaaa acatatactg tataatctga tcaattttag ccatgtcttg
     1621 caatggtaag ttttctattt ttttgagttt tcgttcctta aaatcaagag attttaattg
     1681 tgtaataatt acttgtcctt ttatatctag ctcttcaggt aatgagtaac gtgtcggtaa
     1741 attctttata gtggaagtaa ttggacatat gaccgaaaac attgttttac ggttaaattc
     1801 ataacgagag acgactagtc ctggtcgtct tttttgaatt tcttttccta cggagggatc
     1861 aaaatcaatc catacaatat cacctttttt aggaatataa attctttctt ctgacactat
     1921 aaaatttctc tcccttcagg agttaaatct tcccattcgt ctttctcata atactcggcc
     1981 tcttccccac cactaaatgg atcttcaatt tttggaacta acgtaattgt tccatcatca
     2041 gaatacacaa cgatatattc ttggttttct gaaggtttct ttccgttatc agaaggtaaa
     2101 gttaccacta cagaacttcc ttgtaatcta gattttgtaa caagcattat catcactccc
     2161 ttaataaaag tatactatat aacactaagt aacgcaaaat tcccctttgt aaactatcta
     2221 gaaaattgtt ttgtttaaag tatatcttat tggatataaa tagaaataaa ttgtagccag
     2281 ttcatttttt ctttaactaa aaaaataatc caagaaacat tgaacctatg gatccaaaag
     2341 ctacccaaat actatagcta accccccgct accttcaatt ttgcagataa aacgctttgg
     2401 gagaaaattt caaagacaac aggccatcct atcatgcccg attataaaca aaaagctggc
     2461 tattcctttt gctctgacaa atttcaattg ggaaagttca cgaaactagc aggtgaagaa
     2521 gtttcaactg ttcggatcaa agaatgcccg cttcaaatag aaacattggt tcaacgaatt
     2581 tcaactagac aagactttgc tattgtagaa cgtgagatcg tagctatttt tgtagaagaa
     2641 gggattttac atgatccaac tcatatcaat gtggataaat ggcaacctct tatctataaa
     2701 ttcagagaat atacaactgc caaccagtcg ttaactttga attttagatt ccaagaattc
     2761 aaagagtcat aatgataaac atcgttaggt ataaataaaa aagactcgca gcacgagtaa
     2821 aaaacaaata gaagctatac attagaaagg tcgactgtga ccctatagat gcagtatatg
     2881 cagcttatga gcatttcttc tgttctcttt gtttttggga tctgtgctga agtctttttc
     2941 gttttttgcc gctctttctt tgaaaaatcg attatgttat accttctcca ttactaattc
     3001 aggcatctgc tgagtaatga tttttcgcgc aaaagaaata tctgttaccc aaggttcgat
     3061 ttgttcttca gtcaagacaa gcggcattcg gtcatggatt ggtgcgactg tgcggttagg
     3121 ttcagtagtc attatgattg attctgccat tttttcatcc tgatggctac gataaaaacc
     3181 gccaatatac actatttttt ccttatttga aaacataaat ttgtcttttt cttcattcca
     3241 ctcattggga aaacacaacg attttgcaaa aatggttgat gaaaggtctt tttttcttct
     3301 actgtctctg cacgggcatt aatcatcaat tgtccttttt taaatccggt aaatccccac
     3361 ttcatgattt ccggagtgag gatgttttct tcctcttttc ttaacgtcac aacaagattt
     3421 gacggaaaaa cttctccagc tgctaattct gctgcctgct tcggttgttt cgtctgggct
     3481 tctcgataat aattctgaag gagctctgac tctagatcaa aaaaataacg accacacatc
     3541 ctaatccctc ccaatctttt tttctatcat acactgcaac aagaagttcg caccaacaaa
     3601 acgctttcaa tttttgtaga actaaaaaag ttgtgacaag tgacaaactc tccagtgaat
     3661 ggtattctcc tgccaactcc tcggtcacaa gtcacaatat tcaacaaaca tgagaagctg
     3721 ctttttgaaa ttgttccttg tgactcggag tgaaacggca ggcttacgac ctctgggtaa
     3781 acggtgttca aaagccagtt tctcggtatt aagccaaatt tatgcaaaaa tgtagaaagc
     3841 tattttcaca aaatttttct taatactcga aactaagcga ctttttcaca acctcttcct
     3901 taaacggtgt tcaaaagctt gcatctgcgg taataagccc aaccaaacga aaaatatgag
     3961 cagctatttt tatttggctg ttcttattac ttgatgcagg gcagcttttt cacaacctct
     4021 ccactaaacg atgttcaatc agctaacctt cggtattagc tcaaaaaacg gcaaaatatg
     4081 aggaactatt ttgccgtttg tttctctaat actcaggtca aagcgctgat ttcacaacct
     4141 ctaatacacg gtgttccaga agtagcttct tcgaaaaata agccgaaatt tcacaaaaat
     4201 ttgagaagca attttcgtaa attccttctt atttcttgaa gctgaccact tctgtcacaa
     4261 cctctaatta ttaaagaacc ttttgcgaag ttaacaatct ttctttgatc aattgaaaat
     4321 tctgcctaag tagtgataga tcctgtgtga ctttcaacaa taagatactt ccctggattt
     4381 cagcaatcaa cgctttggct tgacggtctg cttcttcatc ggacagtttt tcttcagaaa
     4441 gtgccaggat attttgtttc gtatacttga cccattgatt gactaattct agtagcaaca
     4501 gacggtattc ttcatccact gtactcaact caataatcaa attacctaca ggacagccat
     4561 gataagtgat ctgtgtctca tgatcagcga aaatccagtc caacatttct tctaatgctt
     4621 cttctggctg ttttccagca tttaagatgt cttcgaacaa ttctgtctgc catacgtcca
     4681 tatgcgcatg gatcaccgct agacaaactt ctttttttga agaaaagtaa taataaaatt
     4741 gaccttttcc tgtatcagaa gctgctagaa tatctcggat tgacgtttca tgatagcctt
     4801 ttttataaat aagatctttg gtcgcttcca agatcttttc ctttgttttc aatgatattc
     4861 tcctttttca tttctcatag tcctatatta tacagtaaat tcatcatttt ttcttcttaa
     4921 tcgtttaaaa aagactgtgc caaattatct gacacagccc tcactataaa actgttctca
     4981 tatagctaga tgttcattga ttcaagaact gcgaggacaa aagcaattcc tatcttatga
     5041 tagttgctct aatacttcct ctgttgtcat cacttgatct gcaataaagc caaagttcgt
     5101 caacgctgca tccattgctt cttggccagg agcaccaatt gcatctgtcg ctaccacaac
     5161 attgaatcca ctctctacca attcacgcat atgggaatcg ctacacaagt ttccgttcat
     5221 tccaccaaga attactgtgt cgattccatt ttttctcaat tggagcgcca aatcattgct
     5281 ttctggtcca aagattttat gaggagagct gatgatcgta ttttcatcca aataaggttt
     5341 caattcttcg ataaaatcgg ctcctgaacc ttcttccact gattcatatt gactttttct
     5401 agcaaacatc tgctgctcaa gcatcatatg ttctcctgca ccgccaaatt gccatttatg
     5461 gtcatgtggg taaaaataat gaggagaaat aaagagtttg tatccttttt cttttgcttg
     5521 ttttaccatt tcaatcatat tttcgatcgt attgtatttt tctaagatat cttttgtcaa
     5581 accaaagcca ccgcctgctg gtttcaagaa ctcgacttgc gggtcagtga gaactactgc
     5641 tgtattttta ttattgaaca tggtggattc tccattcgtt atttatttta atacatcatt
     5701 tagagcttca ctcattgtcg gatgagaata gatttgatct ctcaataacg tataaggaag
     5761 ttttccattc atagctagcg aaatcaagtt gatgacttca tgagaatctt ccgcatagat
     5821 acttgcccct agaatcaagt cagtttcagg gtcgactaaa actttgaata cacctttagg
     5881 atcttctaat acttgtgctt tagggacacc gcttgtcata tacttgaata atttgtaatt
     5941 gacgccttgt gctttggctt gtttttctgt taaacctaca ttggataaag tcggagtgat
     6001 aaatacactg taaggcacag ctttccggtt attcgttgtt cgtttattct cgcccttcaa
     6061 ctgatccata atgattcgat aatcatctag agagatataa gtgaactgca atccgccttt
     6121 gacgtctcca atcgcccaaa tgttgtctgc atctgtacga aggtattcat ccacagcgat
     6181 tgctccgcga tctgttagtt taacatctgt attttctaat ccaagtgatg ctgtacttgg
     6241 ttttcttcca gttgcagcca aaacttttga cgcttggatt gttacttctt ggccatcttc
     6301 tgtaaaggtg acggcagctg ttgattcttg gtctgctact tgatctacag atacacctag
     6361 atggaattcg attcccgcat ttgtcatgtc ttctaaaatc atttgcgcga tatcttcatc
     6421 ttcacgtgga agaaattcag aatgggcatc aagaacgaca actttagatc cgtattcatt
     6481 gaacattgat gcaaattcca agccaatata tccagccccg atgatcacta atttttccgg
     6541 taattgttcc tgatccatcg cttgcgtaga atcaatcaag tagcgacttg tattcaatcc
     6601 tttgattggt aaaatgattg gttcagaacc agtattgatg aaaatccgtt cgccttcaac
     6661 aatcactttt tcaccattat tcgtcacttc gatttgatgg ttagaaagaa aacgtgctgt
     6721 accgtccatc actgtcccag ttgcctcatc gctgatcata tgataatttt tggcgcgaag
     6781 catgcccgtc aatttttctt tccgagcaac agcctctgta aagtcgattc ctctttctcc
     6841 attgaaaatc aatgatttag aaggaataca gccgatgttg atacatgttc cgccatacat
     6901 acgtgttgac ttttcaacga ctaaaaccga ttcacctttt gaagctaacg ccttcgctaa
     6961 agtcttaccg ccctttccaa aaccgatcac aatattttga tatttttcca tcatttttcc
     7021 ctcgcttctt ttttacctac cgattggtac aaaaatatga taaccgataa ttgtctattc
     7081 tccaaatcat ctgctttatt tgtcaaaaaa aaccagacaa aaagcggtcc tttcacgttt
     7141 ttagtctggt tcgatttcct ttattcttct aaacaatcgt tacttctaca tcgatatttc
     7201 ctctagttgc tttggagtaa gggcagaatt catgcgcatc ttctgcaatt ttttgggctt
     7261 cttcttcgct gatgccatcg atatgcactt ctaatactac accgatctga tagccgccat
     7321 catctaaatt ataaagagag acacgtgctt ttactgtgga ctctgctgtc attcctgctt
     7381 ctttgatcat atgttgtacc gccccattga aacaagaagc ataacctgct gcaaataatt
     7441 gttctggatt tgttccttct gcatggattc ctggaggagt gattttcata tgcatcgagc
     7501 catttggtgc ctcgacttct ccattgcgcc cgccatggtt gatcattgct gtttcgtatg
     7561 cttttttcat tgtactgctc ctttcgattg accatttgct acttggctat aattatagtg
     7621 tatgacaagt aaggcgcttt ctacaaaaat tctgcttatt cgaataccag aatgccgtct
     7681 tctactattt ttccaaaaaa caaaaaaata ctactcgtga acaacagcgt catatttgtt
     7741 ccaaaataaa tagcaatctt ttctacataa tcttttatat gattgatttt aagatacata
     7801 tataccagaa agttgacccc ataaaccaat actgttaaaa aaataaccat attcgttacc
     7861 attttttcct ccttttgctg gtctccctac cagcactcga tttaaccata ccactttttt
     7921 tgccgtccaa gcaactagcc tttcaaaaag actggtttat tctgctgtta atgattcaag
     7981 atcagaaaaa tgtccgaata attttgctaa ttttactaac gccaacttgt tctttccgaa
     8041 ttcatcattt tctatctgta atactagtaa cggctcatgt aagcttaaac ggatcaaaaa
     8101 ccagccgttt ccgtatggct cttctagatc atatcgaata ccttcttcat tttccgggtc
     8161 gaagttcatc cctttttctt cttccaacag tcgcttcatt tcttctaata tcttgtttcc
     8221 ttgttcgcgt ggttcatcgc tgaacaattt taatcggatt tcttgtactt ctacgggctg
     8281 tttcaactct ttgatttgtt catctaatgt gatacctttt ctttttaatt ctggcaaaag
     8341 catcaggatc tttgcgatga catatgcacc atcatccaga aaatagtttt ccttgaatgc
     8401 agcgtgtccg cttgtttcga tggctagctg tgtatctttc ccaagacggt taagttctaa
     8461 tgctttattg attacatttc gatagccgga aatataacga acttggatgc ctcctttttc
     8521 ttcaatgaat ctttgtagat gagaagaagt aggggaattc gtcacgattc ttgttcctgg
     8581 atgctctttt aatacgattg tacttaatac tgcgatcaaa ttatttcgat tgatcactgt
     8641 tccagaagca ctgacaactg cagaacgatc cacatctgta tcgaaaatga tcccaagatc
     8701 tgcttcattt gctaaaacag cctgcttgat actttccatc gcttctttat tatcaggatt
     8761 tgggatatga ttaggaaatc ggccatctgg ttccaaaaat tgggaacctg ttgaatccgc
     8821 acctaagcta ttcaatactt tttcagcaaa gaatccacca gcaccatttc cggcatcaac
     8881 aatgattttc caaccattta gtggtttgct ttctagtgtt ccaatcccct ttttaatttt
     8941 tgtgactaaa tcttgcgcat aaggtgtcaa aaggtctgct ttttctacgc ttccagtttg
     9001 ttcgcttttt attttttgct gctgtgatag gataaaagca atatcttctt tttctgctcc
     9061 tccttctgag gtaaagaatt ttattccatt ataataatag ggaagatggc tagcagtcag
     9121 cataaccgca gcatcgcacg agaactgttc aaattgagtg gacataaaca tagcaggcgt
     9181 agtagctaat tcgaaatcga tgacatgtat cccttgttct ttcagacttt ggatcaacgc
     9241 ctctttcagt gctggtccgc ttatcctact gtcatgacca atcccaatcg tccatttttt
     9301 atttggatcg gttgatttct tctttttctt tatccagttt gctagtcccc agccgatctt
     9361 ttttaccatg tctgtcgtca agtttacttc atatccttca actggtaatg cgattcctcg
     9421 aatatccgat ccattttgca gcgctttcat atctttcata taaaagtcct cctcatttat
     9481 tccctcattt tttccagctt ttctgcaaat cgctgtgctt cttcttctga gcggcaaata
     9541 ataatgaatg tatctacacc ggccatcgtt ccgacaactt cagggtaagc cacttcatct
     9601 agccaattcg ttaccacatc ggcaccattt cgttcagtca atacgataac catgaactga
     9661 acagactgga tacgcaaaac ctcgcgtcta attgctgtac gcaaacgttc ttcatttaaa
     9721 gaaacagatg gctggttaaa taatgtatag cgtactattt tgttttcatt ttgacttttt
     9781 actaacttca tttctcgaat gtctcgagaa atcgttgctt gagtcacatc ggcgcctgct
     9841 tcatgcagcc gcgcaagcag ctcttcctgt gtcccaatag gttgttctgt aatgatctgg
     9901 cggatcagtg cttgtcgctg tttctttttc atattagaca cctctcaatg ttgattatag
     9961 caaaagataa gtaatatttc ttcatatttt ggtatttttt acttttattt tttgttttca
    10021 ttcaggttgc taaccttttt ctattttaac acgcaaaatc gtcttcattt tttcaggtgc
    10081 tgattttctt ggatcgctga tgtaaatctc tttgtgttct ttagaagctc tttcatagcc
    10141 ttcttttgcg atgaatgctt ctaatttctc aaacgttgct ggttcttcgg catagggccc
    10201 gacgtgtaag atttgggcac aaagaccttc ttccatcttt ttgaatacta cttgttcttt
    10261 caagtcttcc ggaagtttgc tagaacgctg aatggcttct tgtgctatcg cttctgtaac
    10321 aaagtcaggc tgtcggatca tcaattgata agaaaaatgt tcttttttca tcactggttc
    10381 atgcaaatac ttttcttgca gtccccattg tccttccagt ggatagacag tatacatctg
    10441 ataatcagga atcagccaat tttttttagg cgacatgcgt attgcatagc tgactgcata
    10501 tagacaggct actcttcgtt taaagtcttc ctgattcggg tcgccgatcc ctctgatgct
    10561 aaagaatgat tttttaggaa cctctagtat ttgcgggctt tttttcatac tatagctctt
    10621 gtctttacgc cattcaattt tttccatttt tcttcctccg cgaaattcca tttagttatt
    10681 tattatcata cctctttttg agtgacttgg ctaatcgtgt tgacactatt tttcagtttg
    10741 tgctagaata agtgaagtaa ataaatcgta cagataacac gtttcggcgt gtcgagagag
    10801 aggcggtttc tatttttata atagaaatca ctctcttttt ttacgaaatt ttttagaggt
    10861 gatgtaaatg acaaaacaaa aacagctgct tttagtacct gcagtaattg caacagggat
    10921 gatgtctttt gcgggcgtat tgatcgagac ggccatgaat gtaactttcc caactcttat
    10981 cagacagttt gatttaacga ctgcacaagt ccaatgggta acaacgattt atcttttgat
    11041 gatttcgatc attgttccgc tgtctactta tttgaatcgc aattttagtc tcaaaaaact
    11101 ctttttagct tcaaatctgc ttttcttggc aggtataatg atcgatttct tctctcctac
    11161 cttcatggtc ctattgtttg gtcgattgct ccaaggtgca gcgacaggta tcgctcttcc
    11221 gttgatgttc catattatct tgacatttgc ccccctcaat cgacgaggcg cgatgatggg
    11281 tgtcggtact ttgacaacgg ccattgctcc agcaattggc cccacttatg gaggcattat
    11341 gacttcacat tttacatgga atcatatctt cctgtttctt gtgcccgttt tgattctttc
    11401 tttgattatc ggctggtttg ctattcccga gatggcagta caaaaaagtg gttatttgga
    11461 ttggataagc gttttaggaa tttgtctttt gtttagtgga agtctaacat ttttgagtac
    11521 gttgggaaca gtttccagct ggattgcttt aatcatcggc ggtattggct ggttcctttt
    11581 ttactatcga agtaaaaaag tggagcatcc actgatccga ctgtctattc tttcaaataa
    11641 aacattccgc ttgtttttga ttggcttctt agtttatcag tttttattac ttggtgtatc
    11701 ttttgtcttg ccgaatttcc ttcagatcgt tcaaggaagt agtccttttg tcgctggatt
    11761 agcgatgctt cccggcgcag cggttggtgc tttgctttct cctttttccg ggaaaatgct
    11821 ggatcaatat ggtccgaaga agccaatttt tgctgggctg attctttctt tgattggttg
    11881 gctagcatta ttcctactca taggacacgc ttcagtgggt gtattgattg cttgtcatgt
    11941 gttttatatg atcggtatcg gattatctta cagcaatatg atgaccaccg gaatgaatgc
    12001 actgaatgaa gaactacaag gagacgggaa cgcaatgttc aataccttgc agcagttttc
    12061 tggcgctgtg gctacttcat tagtagcggt cattatcaat tacgtccagc atcatacttc
    12121 ccataactat gaagtgtcta ctacattagg atcaaaagca gcattaggcg tcttgttgct
    12181 gttgcttctc gtaagctttg ctcgattcgc ctattatctg ttctttgcta aaaaagcgta
    12241 gattccatat acggcaggtg ttgcatctgc cgtttttttg tgtgaaaatg gttgtttgta
    12301 aaaggaggct tttttatgga aaaaattgcc attatcggtg gcgggatcat tggtatgacc
    12361 ctagccagtt acttagatac atcaaaattc aatgttgctt tatttgatga agggacagga
    12421 caggcaacta aagctagcgc tgggattatc tccccttggt tgtcgaaacg cagaaacaaa
    12481 caatggtatc gtctggctaa agacggcgct gcttttttcc ctaaacttat aaaagatttc
    12541 caacttgatc aaacgattta cgcacaatca gggacacttt tgcttcgacc tgaaaaagat
    12601 ttagctgaat tggctgttct tgccgagaaa cgaaaagaag aagcgccaga aattggaaac
    12661 atcgaatggc tttcacctga acagacatct caagcacttc cgttattgaa accagcagtt
    12721 tcactgcgta tcagtggtgg tggtcgtctg gatggaaaag catatttaga acatcttggt
    12781 cagcgtttag cacaaaaaca aatggagcag atccaggcaa aagtcacttt gcataagaag
    12841 agcgattctt ggctcatcaa gggaccgttt ggagaataca cagctgatcg tgtaattttg
    12901 acacctggcc cggcacttaa gcctttatta caagaattag gctatgctgc agatatacgt
    12961 ccgcaaaaag ggcagctctt agcatttcag acatcttata agatgagtca agactggcct
    13021 gttgctatgc ttgacggaga agctgatttc attcctttca tggatggaaa gatcctttta
    13081 ggtgctaccc atgaaaacga tcaaggctgg gatttggctc ctactagcga agcttataag
    13141 ggattgacta agagtacaga acagttcttg actgatccag atcagataaa aaactggcca
    13201 tacgagttcc gtgtaggtac ccgtgcttat acgtcagatt tttccccatt tttcggcgta
    13261 ctgccagatg atccaagctt actggctgct agcggtctag gctcttctgg cttgaccaca
    13321 ggtccttata tcggctatct attagccaac ttcttaaata ctggtcattg gcctacggaa
    13381 gattatcaaa aggacatctc cacctatatt acgaaaaatc catcatagaa aaacagcttt
    13441 ttcaagtaag gatcccgagc gatattgcgc atggattact tcttgaaaaa gctgttattt
    13501 tagttcaatt tctgaatcaa attctccaaa ctttcttttg cttcatctgg caagagatct
    13561 tttcctatct gagcttcaat gaatcgtaaa cgaatatcaa gtcgttgttt caccagattt
    13621 ttataggacg tatcctcaaa agaaacagga aaatctggta taggtaaata gtggatgtct
    13681 tcaaatggac caaacgactt gaattctgcc gtttgttgca cgattctttc aacgattgac
    13741 aatgtcgttt caggtttgat ttttttatca tcgaaatatc cagtattcag gacatagcag
    13801 gctgtttgct gtttcaaaaa caactctcga aaagcttgat agtcttccct aagaggatag
    13861 acacggaaag gattagcaaa tggttcaatg actagctcat ctagctgtct ttttgtagtg
    13921 atattttccg cgtctgatcg ttttgttgct aatgttgcac caaaagacgc tgctaaaact
    13981 gggtcattga tcttgataat tggcggaagc gaatcatctt tcatgatcca aaaaacagca
    14041 tcgatctttt cgctaagatg atcgactcgg tcgggtgtcg cataacgtga tttgactgtc
    14101 cgcccatttc cgtttcgtat atctcctgtt actaggactt tttctccttt acttgtacat
    14161 gtcacgccca cattctgaca agtaaggaaa gaagaaatgg ccggatcatc agacggatag
    14221 tcttgtgttt tatcaaagta agaaggttcc aaggcagtag caaaaccatt ttttcgagaa
    14281 ataacgaaag catcgtcatg caagatcgaa acagagtgac cttccgtatg ttttgctaag
    14341 gtaatcgttg attttccaga accggacaac ccaaatacag ccatcgtata ggtctggtca
    14401 ttactagcat attgtttcat cccaccgtgg caggcaataa agccattgcg atttcccaat
    14461 gcccaagcaa gcgtcaaagt cgcttttttt aattctccaa agtaacgtaa tcctagcaca
    14521 gccgctgtat tatggagagc atcaaacaaa atgagtccat ttggaaatgt ttcatgtgtc
    14581 cattcgggat cacaaaagat ataaagatct ccctcatcaa aggaaacaga cttttgataa
    14641 gtctctaaga tcttcgaatt cgctggctga aaattcagca aataagaata caaattgttc
    14701 tcaaatcctt ctggaatcag caaatgactt tgcacagaaa attcttcaga taggccaacg
    14761 attacttttg tatggtaaaa ctggcgcttg cgactttgat acacagcttc tcttaaaatt
    14821 ttctcgtatt tgctttgttc cttgtctaag tcctcaaaga tccgccgagc acttgctgtt
    14881 cttcccacaa tgcttccatc attgaatact agtgtcctaa catcagctgg taaatctagt
    14941 tcttcagcat gtgagattgt taaatctgtg atgatcgttc ctggactccc ttttgctagt
    15001 tgataagctg tcttcctatc aaaaacttca tgaacattat taccgtaaaa tgtcgtttct
    15061 acaatcgatc gaatactgga taacagtgga tttccacttg tgatttcgtt taattcgaga
    15121 ctctcttttg tactcatggc tcctcctcat tccctaaaca aaaaaacgtt ttctttatta
    15181 tacattattc gagtttttat ttgtactgct gcgctgtggg aagcaaacga gttatttttc
    15241 acacaaaaaa accgagaact tgaataaaca agtttctcgg tttcatcatt ttccttttat
    15301 cggcttagta ccagccgttt gctaaccaga atgttttagc agcatcccat gaaccataac
    15361 gagatgttac atattgttct gctacacgtt cttgattggc agctgaatag tcgccgttta
    15421 agtatgaagc atctaattga taacggccga tgtaacgtcc gttagtagct gtgtatgaac
    15481 cgcctgattc tttttgtgcg atccattctt ttgcagagct tgaagttggc gctgttgcag
    15541 cagctggggc agcaggagct acttcaactg tttcaacagg tgcaacgtat gtatgttctt
    15601 gcgttgctgt ttcagttgta gatgctgtat tttctgttgt tttgttttct gttgttgctg
    15661 aagaatcatt tgaagttggg attgttaatt gttcaccaac ataaatgcgg ttgatgtttg
    15721 cgatcgaatt tttttcagca attgcatcga tcattgagtt gtctcctgca aatttttgtg
    15781 agatcttaga taatgaatca cctgatttta ctgtataaac ttcatcagcg tgagcatttg
    15841 tagttcctac aaacattgca gtagcaccag cagcaaaagt tgtacctaaa acaattttct
    15901 taaatgaatt catattaaac tccttttttt ctccgttttt tattctttcg acgctattaa
    15961 ctttatcatt tagaaatatt tcataggtaa aagaaacatg acatttaatg acaaaataac
    16021 gaattattta atatttgta
//
LOCUS       DAZUMK010000056        16018 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000056 DAZUMK010000000
VERSION     DAZUMK010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.057"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            200..868
                     /locus_tag="ACG808_001667"
     CDS             200..868
                     /locus_tag="ACG808_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFS5047293.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1068..1454)
                     /locus_tag="ACG808_001668"
     CDS             complement(1068..1454)
                     /locus_tag="ACG808_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047294.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1426..2808)
                     /locus_tag="ACG808_001669"
     CDS             complement(1426..2808)
                     /locus_tag="ACG808_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFS5047295.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2964..4151)
                     /locus_tag="ACG808_001670"
     CDS             complement(2964..4151)
                     /locus_tag="ACG808_001670"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFS5047296.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4158..5189)
                     /locus_tag="ACG808_001671"
     CDS             complement(4158..5189)
                     /locus_tag="ACG808_001671"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFS5047297.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGHFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5205..6659)
                     /locus_tag="ACG808_001672"
     CDS             complement(5205..6659)
                     /locus_tag="ACG808_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFS5047298.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPTLSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVASASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            6834..7739
                     /locus_tag="ACG808_001673"
     CDS             6834..7739
                     /locus_tag="ACG808_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFS5047299.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENDLQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTGESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7830..8507
                     /locus_tag="ACG808_001674"
     CDS             7830..8507
                     /locus_tag="ACG808_001674"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFS5047300.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8504..8674
                     /locus_tag="ACG808_001675"
     CDS             8504..8674
                     /locus_tag="ACG808_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047301.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8788..9684
                     /locus_tag="ACG808_001676"
     CDS             8788..9684
                     /locus_tag="ACG808_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS5047302.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9739..11109)
                     /locus_tag="ACG808_001677"
     CDS             complement(9739..11109)
                     /locus_tag="ACG808_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS5047303.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11347..12093
                     /locus_tag="ACG808_001678"
     CDS             11347..12093
                     /locus_tag="ACG808_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFS5047304.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12090..12929
                     /locus_tag="ACG808_001679"
     CDS             12090..12929
                     /locus_tag="ACG808_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFS5047305.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12930..13880
                     /locus_tag="ACG808_001680"
     CDS             12930..13880
                     /locus_tag="ACG808_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFS5047306.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14021..14773
                     /locus_tag="ACG808_001681"
     CDS             14021..14773
                     /locus_tag="ACG808_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047307.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14792..15943
                     /gene="queG"
                     /locus_tag="ACG808_001682"
     CDS             14792..15943
                     /gene="queG"
                     /locus_tag="ACG808_001682"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFS5047308.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5189 a         2995 c         3226 g         4608 t
ORIGIN      
        1 cggttgtgta aaaatatcat tttagagcat aaaaaaaagg agtggatttc tccactccaa
       61 aaaagtctaa cttttggggg tcactacagt gtcacagtcc tttttttagt aaaaggatac
      121 atttcttcct tgtatattcc atgcgtgttc gtgagaaagc atatataatt taattaacag
      181 atagttaggg ggagaaaaaa tgattttttc aattacaatg aaactggtgt tcgggttgat
      241 cggtttatta ttagtggtac gtttgcttgg gaagaaatcc atgtcagaaa tcacaccgtt
      301 tgatttagtc tatacgcttg ttttaggtgg gatcttagaa gaatcaacct atgatgacaa
      361 tgtccatgta gggcatgtcc tatttgccat tgcgctttgg gcagtaatga tttatggtat
      421 cgaacgtatc gtccaaaaaa atgaaaaggt caatcgttgg gtcaaaggcg agccatctgt
      481 tctaatcaaa gacggcgtca tcaacatgac agagttgaca aataaccata tcgagatgga
      541 gcagttgcga gcaatcttaa gacagcaaga atgctttttt cttgaaaatg ccaaacatgt
      601 tatcctagag aatgccggtc aaatgagtgt attgaaaaaa tcagatgaag acaaagcttt
      661 atcgatcctg cttgtagatg aagggcaaat tcagcataaa gtgcttcagt ccaatcaatt
      721 gactgaagca tggcttatgg agaacttgaa gaaagaaggc tatgcagatg taaaacagct
      781 gatttatgtt gaatggtcag aagaaaaagg gctctacata ctaaataaag aagacgtcat
      841 gcatgaatct taccgaatag atggatagat agacagaggt tgtgaaatca gcgccttggc
      901 ttgttactca aaactaagcg actttttcac aacctctgat atctaatata cgttgttcca
      961 gaagttgctt cttcgaaaaa taagccaaaa ttccacaaaa atttaagaag caattttcga
     1021 aaaatccttt cttatttttt gaagttgatc acttctgtca caacctctca tgtcacaaac
     1081 catttttttg tttcctttaa ctcaatatac cgtacgtctt cccacaatat cggctgatgg
     1141 tcaatcataa agtaatcttc ataggcttca ccaatgaagt gcccttcgac ctcatccaag
     1201 agtcttccaa attcatcttt taaattgagc tgaatgatta ctgctttacg agataaaaag
     1261 gcttctgtca gtatactatc gatttcttgc aatgtcattt gttgtttagg aagaggctgt
     1321 ttgcgtgctt cttcttcatt ttttttgata ccgttgacta gttcttccat cgcaaaagca
     1381 gtcccccatt ttaatccaaa tggccgatct cgatactcgt tatagtcaat gaattttttt
     1441 gctgtccgtc tcatcttttc caccttctaa tccactcata ccacctgcat gcccgccaac
     1501 gagagaacta cgtgcgatgg cgcgtcctcc ttgcaagaga ctatgcgcat ggatgattga
     1561 ttgaaagccg tatttttttc taatcgtatc gacaacaaaa tcaactttcg cttcatttat
     1621 ctgcttttca ggatcttcaa aaaggttaag ctgtaaacct gtcgcgtata cgaggtcgga
     1681 acagttcacg cctacacgcc gaatatcctg gtagtggtaa tgctggtgga aaatggtcaa
     1741 taaagcatct gctagatctc tgctagcatt tgtcgcttcg attttcattt gctggtgaaa
     1801 cccggtcttg ccatttggat caagatagcc taaagagtat cctacccata agctgactac
     1861 ttgtgttttt gctgcagatc gtctcaagcg agtgccgact tggtcggcca tttctttgat
     1921 aacgatctcg atttcttctc gtcttgtata atctctatct aggatttgac tatttccgat
     1981 actttttgat tttggtgtat attcttgtcc caaaaaactt cgatcgatgc cccaagcatg
     2041 ggcataaagc tgagtaccga tgacccctag cttgcttttc aagtgataat aatccgcgtg
     2101 agctaaatca taaacagaat gtatacccat ttgtttcaga cgttttgctg ttcgtttgcc
     2161 gattccccaa aagtcagtga tatctgagat tttccataac ttttctggta catcttcata
     2221 acgccattca gcaaacattt gctttccgtc ttttgagcca ttgtctaagg ctagtttagc
     2281 aagtagcggg ttatcgccaa ttcctatagt cgtgtagatt ccggtctgct caaacacatc
     2341 tttatggatc agtttagcca tgtcgtacgc atttgtttta ccaaagagtg ccatgctatt
     2401 tgttacatca aggaagcttt catcgacgga atagacatga tggttcgctt cgtctgcata
     2461 ttttttatag atagaattga tttctctgtt cttttccata taataggcca tccttggggc
     2521 aacaatatat aaatcacttg gataggggaa gggaagatct ctggcacggc tgatattggt
     2581 gatgccgtaa gctttttttg ctgcaggact ggaagccaag attaatccgc taccccgtgt
     2641 ttctgtcgaa tcggacggat aggacatgac tactaattta gttgtaaggg gatcaagatc
     2701 cttttcaacg cactctacgg aggcgtaaaa agatttgcag tcaatacata aaatatctct
     2761 tgaaggttct ttttcataaa caaataccgg attttgattc gcccacataa caatcacctc
     2821 ttcaacacaa ttagaacaca cgttctcttt ccgtgtcaat aaaaaacagc aattttcaaa
     2881 tacgagcaaa aggctcacat ttggaaattg ctgagaggtt atttttatct atttgttttt
     2941 agctgagatc gttgattttt ttatcaaaca tgaacagctt ctggatcgat ctttgttgtc
     3001 ccatttccaa tgtgtgcaac ataaaattcc ggagcatagc cgacaacttt catgtattca
     3061 tcatagacat tgtttttgaa ggcaggtata ttttcttctt tgacaagagc aatcgcacag
     3121 ccaccaaatc cagctcctgt cattcgtgca cctaagacac cttcttgttt ttgagcagca
     3181 tctactagtg tatctaattc gatcccagtt acttcataat catagcgaag agagtggtga
     3241 gaatcattta acagttgacc aaatgcttct aagttcccag cttctaattc agcttttgcc
     3301 ttcaaagtcc gctgattttc agaaacggca tggcgagctc tgcgtatcaa tgtatcatcg
     3361 ccgatttggt caatagttga ttcaaattct tcttcatcta agtctcctaa agaagagata
     3421 ggcaaagttt tttgcaaacg tgccaaggct tcttcgcatt cgctacggcg ttcattgtat
     3481 tttgaatctg ccaattcccg ccgcttattc gtattcatga tgacgattgc ataaccatct
     3541 agtttcactg gaaccatttc gtatttcagc gtatttgtat ctaataagat tgcatgatct
     3601 agctcaccga agccgatggc aaactgatcc atgatacctg aattgacacc gatgaattca
     3661 ttttctactt ttttacctgt ttggactagt tccaagcgat ctgtttttaa atcaaacaga
     3721 tcttctaata caacacctac taataattct aatgaagcgc tactggaaag tcctgcacca
     3781 ttagggattg ttccttccac taataactca aacccatgat cgattgagtg tccagcttct
     3841 tttaatttaa ggaccattcc ttttacgtaa tttgcccagt ttgctttttt atcatattct
     3901 agctcatcta aagtaaaact aatgacaccg atatcgtcaa agtttgtgga ataaactaat
     3961 attttattat cgtctcgagg tgctgctaca ccttgcgttc cataagtgat ggaagcgggg
     4021 aagacatggc ctccattgta atctgtgtgt tcaccaatca aattgatgcg tcctggtgag
     4081 aagtattgtg tagttttttt aggtccgaat gtttcggcaa attttgtgtt taagcttctt
     4141 ttgatttcca tgatgagtta ttcctttctt gttttgattt tgaattctgt cactgtttca
     4201 aatactgatc cggcttttaa atgaatcgaa ccaaatgctg agaagcgctc tgcacctgga
     4261 gctgtttgag tctcaaatgt gatgccaccg tgatgtgcca gttttcggtt gcgcatttct
     4321 ggtgtttctg tgccgaaatt tgcagtgaaa ataaccacac tagaagcatc tgtagcaata
     4381 tctacttgga ttcttttatc ggggctagtc aagcgagcgg cttctttacc caagccggtt
     4441 tcttttaaga aaaatggatg atcgattccg tcaacaagat ttttttgatc caaatcacta
     4501 gcaaaaacgg acgctaattt tttaggtatt tgaaaatcga aagcacttcc tgcagcattt
     4561 tcttttaccc ctataggaat actatctgtt cgcaaaggag cataagcttc gctattgagc
     4621 caaagctcat gttgatcgat tgactgagaa gcatctcccg tcaagttgaa gtaaacatga
     4681 tttgtcggat tgaataaagt atcttggtca ctggtgccgc gagaggtcac tcgccagata
     4741 ttatcttttg tcaaagtata gcggatttcc acatccattg tacctggaaa accatgttct
     4801 ccgtcgggac ttgtagtagt aaaaatgaca gaagcttcat tttcgccatt caaaatgaca
     4861 taagaccatt tttttaattc aaatccagga gcaccaccat gcagattatg tccagttttt
     4921 ggatctgtgg ctaattggta agtttttcca ttgagagaaa agtggccatt ttcgattctc
     4981 cctgcggtgc gaccgataga agcaccgata tacggatctt tttcgatata ttcttcagcg
     5041 gtgtcaaatc ccaaaactaa ttctcgatca tttgatttta aactgacgat tctagcacct
     5101 aaatcagaga tagaaagtgc tagcttgttt ttgttctcca aggttatcaa gtgataacct
     5161 tggccaaaat ctttttctgt aatcttcatt ttcaagatcc tttcttaatg ggaaggaact
     5221 actgcatcgt gaagttcatc ttccattgta tcagctggag ccaatttgtt ttccaatttc
     5281 tcaacgattt gtgcatgttt tttctctgtc aatgtaatct tccaagcaaa aataatagcg
     5341 gagaaaatca tcaaagcagc aggagcatag aatgcataag ctttgaaagt cgttacgcca
     5401 tgagcagtga tatcagcagc agtagcatta ccagtcatcc cagaagcgct agcgacaaac
     5461 cctacgatcc cgttagacaa agcaccagcg attttatcta gtaaagggcg aattgataga
     5521 gtaacagctt cgttacggat accatttttc cattgaccat attcaacaga gtctgtgatc
     5581 gtcatcaaag cggataagaa aatcaattgt tgagggaagt agaacaatac tagcccgatg
     5641 ataacgattg gcaaacttga acctgcgatc gtaaagaaaa agtaagaaga aatcatcatt
     5701 gcgatccctc caaggtaaac gtagcgacga gagatgacgc gtgtaagcat tgggaatagt
     5761 ggaacagcta ctaaaccaag taaggcagca actagaccaa cgatccaaaa ttcagttggt
     5821 tggccaagta catatttgaa gaagtagaat aatacggcag ttgttgctac attggcaatc
     5881 gcataagcga tataagcaag ggcaagccac atcaactgat cattttgagc aactgctttg
     5941 aacacatctt tgatcccagc tttttcattt tgctgacgga tcgcagtatc gacttctttt
     6001 gtgcccaagg cagtgataat cgcagtaccg cctgaaataa cagcagcaat cacaccgaac
     6061 cagaaccagc ctgaagcgcc ttcaatatgt ttatgagtaa caagataggt aaagaaagta
     6121 acgataggga caacgactag agtagtacca tttgcaccta gagaagagcc gaaacgagca
     6181 aacgtagcaa atttttcacg ttcctttgtt tccgtactca aagttggaat cattgaccaa
     6241 aaagcgatat ccttaaatga ataaaaacta tccaataaga tgaaaacgat tgcaaataat
     6301 atgataaata aagtcgtatt atgagtagct aagccaaaga aattggtaaa gattgctacc
     6361 aagagtacgg aactaacaac gccgccgata actaaccatg gtttgaattt accgtatttt
     6421 gtacgagtat tatcgatgat gccaccaatc aaaggatcaa aaataatttc aactaagcgg
     6481 atacctacaa ctaagcttgt aactgtagca atcatttttg cattcgttgc cttatctgtt
     6541 cctgcgaaca tctggcttgt cacgaatacc ataaaataag tgcttaatgt aacatagaat
     6601 gtgtcgtgtc caaaagcgcc taaagcgtag gacacacgtt ggatcatttt gcttttcata
     6661 atctgtattc ctctcctgtt caatatataa tactaagtaa accggttaca acgaataaca
     6721 aagatctttt aatgacttaa acttaacaag aaatcgcttc ctttttaata tatttctgtg
     6781 acttttattt aactttttgt tgcattatac taaaggagta aggagggata gacgtggagc
     6841 aatcgatttt tttaaaaaag gaacatcaac aagtagaaaa agaattgtat tttgattttt
     6901 gtggtttctc taaaacgatg ccgtttcatt cttttggtcc tgctattcgc aatgattaca
     6961 tcctgcatgt cgtgatggaa ggcaaaggat cttatcatgt gaaaaatcaa caattccaat
     7021 tgaaaaaagg tgatctgttc ttgattcgtc caggagactc gacgttttac ttatcagatg
     7081 gagaagatcc gtggatgtat tgctggcttt catttggtgg gagtgctgca gaagaaatca
     7141 ttcggcagtc gcttttcaga gaagaccaat acacgatggt ctcttctgac atacaaaaat
     7201 atatcgacat tattttagaa tgcatgaaag atacggaaga aaccttggaa aatgatttac
     7261 agttaacagc attaacctac cgttttttat cagtcctttt aaaagatggc ggaaaggtca
     7321 gcggtggtgt acaaaaggtt cattcctctt tagctgttga aacagtcaaa tacatcgagg
     7381 cacattatgc tgaaaaattg tctgttgagg aaatcgccca gcatttagcg gtcaatcgga
     7441 gccatttgtc gcgagtattc aaaaatcata tgggtaccag tatcaaggaa tatttgatcg
     7501 gggtacggat caatcgaggg gcgtttcttc tttctctgac gggtgaatcc gtggaaagta
     7561 ttgcctatca agtagggttc aatagtctag tcgtatttag ccgaatgttc aaaaagagta
     7621 ccggagaaac ggctacgaac tatcgtaaac gtatgaaaaa cgaaaatttt aagaatattg
     7681 ctttggatac cttgaaaaaa cagttggaaa aacaaaccgc agtttcttgg acaacctgat
     7741 tagtctggtt aaaatgacaa gcatcggcaa aagaaacttg tggcggttat gctacactgt
     7801 aaagcgaaga ctaaaaagga ggaaaaggga tgcgtacact tatcatcatt agccatccaa
     7861 cgatcgaaga atctttaaat cagcaatttt tcaaagaagc ttctgctggt ttaccagcta
     7921 cttggcatca tttggaatca agttatccag acggaaaaat agatatagag actgaactac
     7981 agctcttggc ccagcatgat cggatcattt tccagtttcc gttttactgg tatagttctc
     8041 ctgctcattt aaaaatttgg caagataatg tcttggaacg agcagcacat gtattaaaag
     8101 ggaaagaact aggaatcgta ttgacaacag gtgtggcgga aaaagaatac caagctggag
     8161 gaaaagaaga atatacgatt tccgaatttt tgaggccgta tcagcgaatc gcaaataaat
     8221 tccatatgac ctatcttccg ccattcgttt tagcacaatt tatgtattta tcccaggaaa
     8281 aacgttggga gaagctaatt gcttatcagc agtatctctc attggaaggc aaaccatcgc
     8341 tgactcagcg gattgactgg tttattcaaa gagtacagga aaatcagaaa atgcaggaag
     8401 aagaatctga aaaacaagca tatatcgtag aagcactgac tgaggcaaaa gagcagatcg
     8461 aagacttgac ctttacactg caagaaatga aaggaacttc attatgacaa atgattggct
     8521 gatccaacaa ttagatgaat taagcgaaaa atcagaatat caagaccgtg cgctcttata
     8581 tgaaataaag aaactgatgg ctgagataga cgaacgaaca atgcagctgc aaggagaaat
     8641 cgacggacaa tcttggaatc atctcaactg gtgaaatttt cttataaata acaaagataa
     8701 aaatgtgcac agcgacaatt atttgctaca ataagaatat gaagcgtttg ataacgctat
     8761 tatacataaa aattggtggt gaaaaaaatg tatgatgaaa atgagtgggg agaaaatgtt
     8821 cggatgaccc gaagaagaca gcaaaaacag gcagctcgta agcggatgat catgattggg
     8881 gcagcagttg ccgtgttttt gatcatagca ggaggagttg cgggtctata ttttcatgga
     8941 aaaaattcgt cagaagcgaa aagtacgacc ataacggaaa caacgacaaa gaaaacagag
     9001 acgaaacaaa caagtgagtc atcaactaaa acaaaagaga cacaaacttc tgaagaaaaa
     9061 acagcaaatg gtttgccaga gcaacttcaa gcaatcgtaa atgattattc gaaaaagatc
     9121 gaagaaaaga cgcctgcatt gattgaagaa taccagacag aaattcaggg aaatcaagag
     9181 ggaatagctg gtttatcagc agttgctaac caaaaagcac gtgagcttca agcaatctcc
     9241 gacgaaggga tcagaaaatt acgtgcagcg tatcaggcag cagaaaataa agacggtgta
     9301 gacttagata ctttgatcaa tcaattatcg gctaattata cgaatcatgt cgctaagatt
     9361 tccgatattt atttgcagac tagcgcctct cttcaagcag agtcaacaag cacacaagac
     9421 acaacatctt ctgattctga gacagcagag tcggtagaag cttctgaaat gcaagctcga
     9481 acaacacaag acacaacaga ttcctctgaa actaatacca acagcgcgac ttcacaggct
     9541 gcgacaaccg tagttttgga aggagaaggt ccaaatcaaa tagccgaacg aactggcgta
     9601 ccagtagaaa cgatcttgtc gctgaatggg atgagcatgg agaattattt cctgactccc
     9661 ggtgagacgc taaaattgaa ttgataaaag caataaaaaa gaacgaagct tccactaagg
     9721 tgaagcttcg ttcttttttt attttccggt tcttttttcg cgtacaaagt aatggatcgt
     9781 catgtaaagc aagaaaacga gtccaataat caatgagatt ctcgtttcag gattgataaa
     9841 catgaaaatc aatacgatag ctaaaaaagc aagtgtcaca taattcgtat aaggagatag
     9901 tggcatttta aaaggatgat catgcatttt atctgcgtgc ttctttttga agcgaatctc
     9961 gctgatcaaa atcactaccc aaggaaccat accaggcaaa atacttgagc tgtatacgaa
    10021 aacgaaaata ttttctgact ggttgatgaa cattggtaga agataattta agatcagacc
    10081 aattaagatt ccacctgaga ttgctacgac aggataaaaa ggcgcaccgt gacgagatag
    10141 tttcagaaat tttttagaca tctttccatt tatggcaagt gtatagacca tacgacttgc
    10201 gctgaaaatc cctgaattgc aacctgataa tgccgctgta atcacgacaa aattaatcag
    10261 tcctgcagca aatgtaatgc cgactttaga aaaagtttgt acaaaaggtg aaccaatctc
    10321 actcaattga ttccacggat agatactcac aatgataaaa atagcaccaa tataaaaaat
    10381 caagattcga ccgatagtgg actggacagc ttcaatgatc gtctttttag gattctctgc
    10441 ttcaccagca gtcattccga tcagttcgat tccttgatag gaagcaacaa cgatcgataa
    10501 tgcaaagaaa aatcctttga cgcctccagt gaagaatcca ccatttttcc aaagatttga
    10561 aatccctact gcctgaccat gattaccaaa tccaaataag attaccccaa gacctgcaat
    10621 gatcatcaaa atgatcgtta tgactttgat gatcgaaaac caaaattcta attcgccaaa
    10681 cgccttgaca gaaataagat ttgccagcat aagaaacaag acgacaatga ttccgggaat
    10741 aattgccggc aaatcaggcc accaataatt catataagat cctacagcaa tcacttcact
    10801 tattcctacg acaatatatt gaaaaatatt actccaagcg gtcaagtagc cagccacagg
    10861 atggatgtac tcgctcgcgt aatttgcaaa agaaccagtc gaaggatcaa cgtaaagcat
    10921 ttcgcctaaa gcacgcataa tcaaatacaa aaagaatcct gcaatcgcat aggctcccat
    10981 cactgagggc cctgtccaac gtatggttga ggaggcaccc ataaacagac cgacaccgat
    11041 cgttccacct agtgcgatca tttggatatg acgggcacta agacttcgag ttaacttttc
    11101 ttcttccata caaaaactcc tttcgttgtt gagtcaacaa gaaaaaataa tcaaacgttt
    11161 caacacatgt ttttcattat taggttagcc ttattgaact cttgatgtga ttctacagaa
    11221 aattttcata aaatgcgagg aaaattcgaa aaaaactttt tcatcattct acttgaaaat
    11281 gctttccatt ttagttaaac tatatttaaa taaaaagtta ggtaaaccta aaaaggagtg
    11341 taagatttgg aacgtacggc cattgtaatt caagaccttt cagccgctta tcaagggaaa
    11401 actgttttga aaactatcaa cttgacgata aatccacaaa aaattaccgg tattatcgga
    11461 ccaaacggtg caggaaaatc aacatttatg aaatctttgc tagagttgat accagctact
    11521 actggtaaag tcaccttttc tggacagtcg gtaaactcag tccgtaaaaa aatagcctac
    11581 gtggaacagc gaagtgaatt ggatctttcc tttccaattg atgtattagg cgttgtactt
    11641 ttaggaacat atccatcttt acgaattgga caaagacctg ggaaaaaaga aaaagaacgt
    11701 gcaagacaag ctttgaaaaa agtagggatg gaagaatatg ccaaaagaca gatcagcgaa
    11761 ctatcgggtg gacagcttca gagagttttt attgcaagag ctctagccca aggagcagaa
    11821 tggatctttt tagatgaacc attcgtaggg attgatgcgt taagtgaacg aaagatcttt
    11881 gacatcttgc aggaattgaa gaattcagga aaaacgattt tgatcgtcca tcatgatctt
    11941 cataaagtag aagaatattt cgatgaggtt attcttttaa ataaacagct gatcgcttcc
    12001 ggtccagtac aagaagtctt tacatcgaag aaccttcaat tggcctatgg tgagatcatc
    12061 agacatttag taaaaggagg agagaaaaaa tgatccaatc ctttattgat ggattaatga
    12121 attatcaatt cctgcaatac gctttggtca cttccatgct tgtaggactt gcttctggag
    12181 tcatcggttc ttttattatt ttacgaggga tgtctttgat gggagacgct atctctcacg
    12241 cagttcttcc aggtgtagcc atttcttata tgtttggttt tagttacatc tttggagcca
    12301 ctgcttttgg gatgctaaca gcagcagtga ttggtttcgt gactcagcgg agccgattga
    12361 aaaatgatac agcgattgga attgtcttta gctccttttt tgcattaggg attatcttga
    12421 tttcatatgc ccagagtgcg acagatttgt atcatatttt atttgggaat gtcttagctg
    12481 ttagggaaag cgatttgttg ctgacagcac tcgttagtgg aatcgtcttg atatttgtct
    12541 ttttctttta taaagaattg aagatcacat cattcgatcc gacaatggca aaagcttatg
    12601 ggctgaacac atcgctgatc cattatcttt tgatgttctt tttgacatta gtggctgtag
    12661 tcagtttaca gacagtagga acaatcttgg tgattgccat gttgatcaca ccagccgcca
    12721 cggcttactt gctaacgaac catttactga aaatgatcat tacagctgca ggaatcggta
    12781 tgctaagtgc agttgtcggt gtgtttttca gttatagtta caattggcca tcaggagcta
    12841 cgatcgtgtt agcatgtacc gcatttttta tccttgcttt tttattttct ccaacaaaag
    12901 gaattttatt tcagaaagga agcagataga tgaaaacaaa aaaatcactc tttttaatct
    12961 tggcagttag tttcttggta ttagccggtt gcggaaagca ggcttctgac caagcggatg
    13021 aaggaagtaa agaaaagttg tcagtcgtgg ctaccaattc gatcttggcg gacatggcaa
    13081 aagaggtcgg aacagatcaa atagatatcc acagtatcgt accagtcgga acagatccgc
    13141 atgaatatga agtactacca gaagacatca aaaaggcaag cgatgcagat gttattttat
    13201 acaacggttt gaatcttgaa acaggtaaca gctggttcga taacttgatg gaaacggcta
    13261 aaaaagaaga aggaaaagat tattttgcag ttagcaaaaa tgtagaacct ctttatttaa
    13321 ctagcggtga agaacataca aaagcagatc cccatgcttg gctagaccta tctaacggaa
    13381 taaaatatgt ggaggaaatc gcacgtatat tctctgaaaa agatgcagaa aatgcgacac
    13441 tctataaaaa aaatgcagaa gcatatgtgg aaaaactaaa agaattagat acacaagcga
    13501 aggaaagttt tgcttctatc gaagagaaca aaaaattatt agtaacaagt gaaggggcat
    13561 tcaagtattt ttcacgagca tatgatttgc cagcagctta tatatgggag atcaatacag
    13621 aaagtcaagg tacgcctgat caaatgaaag cgatcattga tcagataaga gcaaaagaag
    13681 taccggtttt attcgtggaa accagtgtag actcaagaag catggaacgg gtagcaaaag
    13741 aaacgggttt gaaaatctat gataaactgt tcactgattc catagcaaaa gaaggagaac
    13801 aaggagattc ttactatcag atgatgaaat ggaatattga aacgatccat gaaggtttgt
    13861 cacaaacaaa agaaagctga aaagtgcaag gtctagcttg aggagcagac cttgcatttt
    13921 ttataagaga tatgaaaaaa aattatgctg tttttcgtcg tacagatacc ttttttgaag
    13981 aaattccgtt atagttagag taaggaagga aggtgtggct atgaatttta cacgtaaaga
    14041 taaatatacg attaaagaag aagtctttcg attgatcagc cagcaggaaa ataccgacaa
    14101 ggatcaagac cttcaatttc ttgaagcaga attagctaaa ttattcatag caaatcaaaa
    14161 tgagacaaca gaaccagatg aagagctgta cataggcaaa tggttgtttg atgaatttca
    14221 gcctttagat gaaaataccg gtatcggtta ttttgtgttc ctttctccag tcggcttcac
    14281 acctggcgta ttagttaaac aaaacgacga aataaccgtc aaagcatggc ctgcattatt
    14341 ttcagtcatc aaagcagaag gagtaatcaa aaagatccag tcggcctatt ataaaagtca
    14401 atttattgcc aacagcaaat taatgaatat gttttataat cgtctagtag aaattgctag
    14461 gcctgggaaa atcccgcaaa cattcacgta cacacagcaa aaaaatctct atgaagaagt
    14521 attgtattcc cgcgatctgt ctgagttttc gccatgggaa gaggtaaggc tccatgaact
    14581 gttaagtaca aaaccagttt cattgaattt ttttaatgca ttagataaca aaagaaaatt
    14641 aatttcaaca aattattggg aagtatttgc tgcaagaaaa aaatggatgg aagaaaagaa
    14701 gattgccgat cagcagatcc agtttgtcca gctgaaaaaa gaaaaactat attatgggtt
    14761 atacagccgt taacagcaag cgaggaagac catgacatta aaagaagaaa tcatacaaga
    14821 aagcaaacga ttaggaattg acaaaatcgg gttcaccacg gcggaacctt ttgattcatt
    14881 aaaagaatcg ttggaagaac aaaaagcagc tggccatact tctggctttg aacatccaaa
    14941 tatcgatgag cgtctttatc cagaaatgac atttgatcag ccgaaatcga tcatttccat
    15001 tgcacttgcg tatcctacta ggatccatga agaagtgcct agggatgaaa aacgcgggca
    15061 gtttgctcgt gcttcttggg ggatcgatta ccatgatata ctcagagacc gtttgaaccg
    15121 attgatcgaa ttcatccaaa caagagcaga aaaatggcaa aaagaagagg aatggcgtct
    15181 agctccgcaa gtagatactg gagaactgat cgatgtcgct gtagcacagc gagctggttt
    15241 aggatttatt ggacgaaacg gattgttgat cactgaagag ttcggttcat ttgtctatct
    15301 tggtgagatc gttactaata ttgtatttga gcctgataaa ccaggagaat ttggttgcgg
    15361 tgactgtatg cgctgcgtga ctgcttgtcc gacaaaagca ttgcttggtg acggaagaat
    15421 gaatgcccag cgatgtttgt cttatcaaac tcagacaaaa gggatgatgc cggaagaata
    15481 tcgtaaaaaa atgcgaaatg tcatttatgg atgtgatatc tgccagttag tctgccctta
    15541 caatcaagga aaagattttc attttcatga agagatggaa ccaaaagtag acgaaatata
    15601 tccaaaatta aagccaatgc tttcgatgtc taataaagac ttcaaacagc aatttgggca
    15661 tttagctggt tcatggcgtg ggaagaaacc tttgcagaga aacgcattga ttgctttagc
    15721 taatctaggt gatcgaacgg cattgcctga gatttcagaa tgtttaacag atgtccgccc
    15781 tgtcatcaga ggaacagcag cttgggctat tggacgtcta ggaacaaaag aaccggaaaa
    15841 atggcttgat gtattagatg aaatattagc taaagaaaca gaagaagtcg ttataaacga
    15901 attggaacat gcccagaaat tattgcggag aaagttaaaa taaaaaaata gttaacatta
    15961 ttcaaagttt ttgtccagta attgttgtaa caattactgg tttttttgta ttttttac
//
LOCUS       DAZUMK010000057        15400 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000057 DAZUMK010000000
VERSION     DAZUMK010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.058"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..833
                     /gene="mreC"
                     /locus_tag="ACG808_001683"
     CDS             <1..833
                     /gene="mreC"
                     /locus_tag="ACG808_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFS5047309.1"
                     /translation="NIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIVNDSVAMVD
                     KTISAPVRWVRNGVASVHNLFSTYAENERLKEKIDSYDDVSQQNKNYEREIEALKKEL
                     ELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGLIGRVIEVN
                     TASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLTGDTDIKEG
                     DVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVTIVQRLAED
                     "
     gene            837..1346
                     /gene="mreD"
                     /locus_tag="ACG808_001684"
     CDS             837..1346
                     /gene="mreD"
                     /locus_tag="ACG808_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFS5047310.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            1601..3175
                     /locus_tag="ACG808_001685"
     CDS             1601..3175
                     /locus_tag="ACG808_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFS5047311.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(3243..5042)
                     /locus_tag="ACG808_001686"
     CDS             complement(3243..5042)
                     /locus_tag="ACG808_001686"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFS5047312.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            5165..5671
                     /locus_tag="ACG808_001687"
     CDS             5165..5671
                     /locus_tag="ACG808_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFS5047313.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            5750..6472
                     /locus_tag="ACG808_001688"
     CDS             5750..6472
                     /locus_tag="ACG808_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5047314.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(6550..7950)
                     /locus_tag="ACG808_001689"
     CDS             complement(6550..7950)
                     /locus_tag="ACG808_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFS5047315.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(8120..9094)
                     /locus_tag="ACG808_001690"
     CDS             complement(8120..9094)
                     /locus_tag="ACG808_001690"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFS5047316.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            9447..10007
                     /gene="pth"
                     /locus_tag="ACG808_001691"
     CDS             9447..10007
                     /gene="pth"
                     /locus_tag="ACG808_001691"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFS5047317.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            10024..13545
                     /gene="mfd"
                     /locus_tag="ACG808_001692"
     CDS             10024..13545
                     /gene="mfd"
                     /locus_tag="ACG808_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFS5047318.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            13560..15158
                     /locus_tag="ACG808_001693"
     CDS             13560..15158
                     /locus_tag="ACG808_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFS5047319.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIRRL"
     gene            15240..>15400
                     /locus_tag="ACG808_001694"
     CDS             15240..>15400
                     /locus_tag="ACG808_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS5047320.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALE"
BASE COUNT         4768 a         2881 c         3273 g         4478 t
ORIGIN      
        1 aaaatattat tattaccttg gttattgtta tcatcgtggt gacgatacta agtgtgaccg
       61 ctgcgcagcg agcgaatgaa ggaaagccca atttcttcca atccatcgtt aatgacagtg
      121 ttgcaatggt tgataaaaca atctctgccc cagtacggtg ggtacgaaat ggagtagctt
      181 ctgtccataa tcttttttca acttatgccg aaaatgagcg gctaaaagaa aaaatcgata
      241 gctatgatga cgtttcacag caaaacaaga attacgagag agaaatcgaa gcgttgaaaa
      301 aagaacttga gttgaatcag acgctgacta gttacgaaaa agtaacggca aatgtcatta
      361 ctcgttcgcc tgacagttgg caggacatgc tgatcgtaga caagggttct aaagacggta
      421 tcgaagcaaa tatggcagtc atgtcgcaaa aaggattgat tggccgagtg atcgaggtca
      481 atacggcttc gtctaaaatc gaattactgt catcctctaa tgaaagctcc aatcattttc
      541 cagtacgggt atcttcggct aatggcgaag cgtttggttt gcttaaaaac tatgatgaaa
      601 agctccatgc cttagtggtg acccaattaa ctggtgatac ggatatcaaa gaaggggatg
      661 ttgtccagac atccggtctt ggagggaatt ctccagctaa cttgccgatc ggtacggtta
      721 ttaaaacgaa accagatagt tatgggctgg atcgggaagt ttatgtgaaa ccttatgcag
      781 aaatgtatga cgtgtcagtt gtgacgattg tccaacggct agcggaggat tagtccatgt
      841 tgaagaaaga aacaatgaag tactatctgc caatcgtttt gttctttttg atgttgatag
      901 atggtcattt aacaagaatg ctaggggagt ggtcgaaagg cacctatatg tcaaatgccc
      961 actttctgat attggcatta ttatgttgca gtatggcgtt tgaaaaacgt tatttactga
     1021 ttaccacgat tgttctcggg gctatctatg acgcttacta tattggcgtt atcggtatct
     1081 atgcagtagc tctcccttta attgtatggt tgatgtatgt aatgaaagac gttatccatg
     1141 tcaacatctt tactgaattt ttcagtatga tcatctttgt cacgggttat gaattgttta
     1201 cgatggtggt ccagttgatt tttaaattag cagtagtaaa taacacgtat tttattacaa
     1261 ggtttttagg acctacactg ctgttgaaca tgattatatt tgtattattc atttttccct
     1321 ttaagaaatt attcagtgat gaataatttc tttttttatt tttaggtcga aagcgaaaaa
     1381 gaatcgttgt tatcttaaga aatgctagac ttaacgcggc tttttcatga tcattttctg
     1441 aaacgaacag ttttgtcatt tttaaaagtt gttttgaaac atttttgtaa cataactatt
     1501 atatcgttga cacactgcta tggtacaatt tttatatcgt taagaaagac ttaaatatat
     1561 ttagatattt attaaattaa gccagtcgga ggaatgaaga gtgaaaaaga gtttaatatc
     1621 agcagtaatg gtatgttcaa tgaccttgac tgccgtagca tcaccgatcg ctgccgcggc
     1681 ggacgatttt gattctcaga tacaacagca agatcaaaag attgcagact tgaaaaatca
     1741 acaagcagat gcacaatcac aaatcgatgc cttagaaagc caagtttctg aaatcaacac
     1801 acaagcacaa gacctattag caaaacaaga tacattgcgt caagaatctg cacaattagt
     1861 aaaagacatt gcagatttac aagaacgtat tgaaaaacgt gaagatacaa tccaaaaaca
     1921 agcacgcgaa gcacaagtaa gcaacacaag ctcaaactac atcgatgctg tattgaatgc
     1981 tgattcatta gcagatgcta ttggacgcgt tcaagcaatg acaacgatgg taaaagcaaa
     2041 caacgacttg atggaacaac aaaaacaaga taaaaaagct gtagaagaca aaaaagctga
     2101 aaacgatgct aaactaaaag aattagctga aaaccaagct gctttggaat ctcaaaaagg
     2161 cgatctttta tctaaacaag ctgatttgaa cgtactgaaa acttcattag ctgctgaaca
     2221 agcaactgct gaagacaaga aagctgactt gaaccgtcaa aaagcagaag ctgaagcaga
     2281 gcaagctcgt attcgtgaac aacaacgtct agcagaacaa gctcgtcaac aagctgcaca
     2341 agaaaaagct gaaaaagaag cacgcgaaca agcagaggct gaagcacaag ctactcaagc
     2401 ttcatctact gcacaaagct cagcaacaga agaatcatca gctacacaat cttcaatgac
     2461 tgaagaatca tcatcagcta cacaatcttc agcaacagaa gaatcaacaa cacctgaatc
     2521 ttcaacagaa gaatcaacag cgcctgaatc ttcagcaaca gaagaatcaa caacagcgcc
     2581 tgaatcttca gcaacagaag aatcaacaac agtgcctgaa tcttcagcaa cagaagaatc
     2641 aacaacagtg cctgaatctt caacaacaga agaatcaaca acacctgcgc ctacaacacc
     2701 atcaacagat caaagtgttg atacaggaaa cggcacagga agtagtactc cggctccaac
     2761 gccaacacca acacctgaac aaccaaaacc tgtaacacct gctccagctc cttctggaag
     2821 tgtgaatggt gcagcaatcg tagcagaagc ttacaaatat attggtactc catatgtttg
     2881 gggcggtaaa gatccaagtg gctttgactg ctcagggttc acacgctatg tttacatgca
     2941 agtaactggt cgtgacatcg gtggttggac agttcctcaa gaatcagctg gaacaaaaat
     3001 ttcagtttct caagcaaaag ctggagactt gttattctgg gggtcacaag gcggaactta
     3061 ccacgtagcg attgctttag gcggaggaca atatatccat gctcctcaac caggcgaaag
     3121 cgtaaaagtt ggttcagttc aatggtttgc ccctgacttt gctgtcagca tgtaagaatg
     3181 attaatcaaa aagagttagc ccattctgga ctaactcttt ttttattgtg ataaaataga
     3241 aatcagataa attcttctgt caattccatg aacgtttgga tatcttcagt caccatgtcg
     3301 atacctgctt gccaaaaatc tggttgggta agatcgacat tcaaatgttt cttcgcaaga
     3361 tcttccgtcg tcattgaggc agtatcgcgt agtaaatgga tatattcttc ttcaaaattt
     3421 gctccctttt ggttggcgta agcatagatg cccaaactaa agaggtagcc aaatgtgtat
     3481 gggaagttat aaaacggtac gtcatcaata aagaaatgca attttgcagc ccagaaatat
     3541 ggatggtaag tagctaaagc tccttgatag ccttcttttt gcgcctcaac catcatctct
     3601 gtgatcttat cttctgagac taatccttct tgccgtgctt catagaatcg attttcaaaa
     3661 atgaatcggg aatggatatt catgaacata gctaaagcat tttgcagttt tgtatctagc
     3721 aaattgattt tttcttcttt tgtttttgcg acttttaatg tagcatcggc tacgatcagt
     3781 tctgcgaatg tacttgctgt ttctgcgaca ttcatggcat attcgcgatt taaagaaggt
     3841 aaatcccaca tcgtgctgct atggaaagca tgtcctagtt catgagcaag tgtcgcaacc
     3901 tcattaacag aattactgta ggtcataaag atccttgatt cttttgtttc tggtaactca
     3961 gtacaataac caccaggacg tttcccagga cgatcttctg cttcgatcca gctcttttca
     4021 aaagcagatt gggcaaattt agccattttt ggactgaatt tgttaaagtt ttcgataata
     4081 aatgctgctg cttcgtcaaa actaaaggtt ttttctttca aatcgcctaa aataataggt
     4141 gcatcttgat cttgccattc cattttttct ttaccgaaaa gattagcttt tctagtcaag
     4201 taattaacaa ggggttgttt gtttttctgg attgtatccc acatgacaga caatgtttct
     4261 ttcttcaacc gattgtactc taatggtttt tgtagaaaat ctgtgacacc atgaagttca
     4321 tagtcagaaa gtctaaaacc atctaaatga ttcaaagtat ccgtgaagag gggtgccttt
     4381 tctttccagg cttcttccca tgctgcgaaa agtgtttctc tgacttttgg atctggatcg
     4441 cccatcatct tattgaaggc ttgtccagca gataattcga ctacttgtcc atcttgttcg
     4501 aagggaatcg aaatgctggc tacaatcgta tcataatgac tgctccaagc atttagacca
     4561 tctaaagaaa gcgtatttat aatgttttct tcagcttctg ataataattg tgagccatca
     4621 cgacgaatct cgtttaaacg aaaagcaatt gtttcaaacg aagattgaga aagcatctga
     4681 ttccaatcat tgtcagatat ttctgcaaat ttctttgaca atacagtttc ggctgattgc
     4741 agacgaggca gcattgcgta aagcttgcct gataagacct ttgcatctga atcattgaca
     4801 ttggcagaaa gcaatgcagt aatatagctg ctgcattggg taaaaccatt cgtaatcgtt
     4861 tcttgcaatt gaagaatagc gtttaactgc tctgcattgt cagaactagg tgaccatttt
     4921 gttacgcggt gataatactc gttcatttgg tcttccaatt gcttcatccg ttggttcaaa
     4981 gcatcagaat gactgcctcc ggaaaaaata gaatctagat tccaatttaa tgaataactc
     5041 atgaaaaaac tcccttcata ttgtaataca aaattaagta tagcactttt ttatggaaga
     5101 agtctctatg aaaatgattg ttgtttcttc aaaaaaagtc taagataaaa tacagaggtg
     5161 acttatgaat tcttcaaaaa aacaaatgaa aaacggaaat atttatttag taatcgcact
     5221 gatagtcgtt ataatcttat tttacagttc ttctcaaact tacggacagc aatcacaagt
     5281 ttcgcgatta gaaagctggc tgcctaatca gccatttaaa gacacattag cgggtatacg
     5341 ttttaattat ggtggaagta tggtgagtat cgaccatctg ggctacgcca aatttttaga
     5401 gttcttttta aggaagggcg cacatttcgg tacctatttt attttgggag gcagcttgtt
     5461 tttaggtgtt ttccccaagc taaaaatttg gtggctgaca gcgatccttg cttggctttc
     5521 agcaacaggg tacgcaggtt tggacgaatt ccatcagatg atgaccggtg gacgaacacc
     5581 tatgttccaa gatgtcatgt tggattcgat cggtgctctg actgctgtga tattatgtgt
     5641 gctttttgcg gcattcaaga agcgtttata gattggctgt ggctttgttt ttttgtgaaa
     5701 tagtactaga atagaagtgt attctcaaaa gaacaaggga ggaaaaatca tgtcaggaca
     5761 tagtaaatgg cataatatcc aaggaagaaa aaatgcccaa gatgcaaaac gagggaaagt
     5821 ctttcagaaa ctttctcgtg aaatttatat ggcagctaaa gcaggcggtc ctgatccatc
     5881 tatgaatcca gctttgcggc ttgttgtgga caaagcaaaa gctgcaaata tgcctaacga
     5941 caatgtggaa cgggcgatta aaaaggctac gagtgccgta gatgaaacga attacgatga
     6001 aatcacgtat gaaggatatg gtccgggtgg agtaggaatc ttagtccatg cattgacaga
     6061 taaccgtaat cggacagcga caaatgtccg agtagctttt actcgaaacg gtggatcact
     6121 gggagaaacg ggttcagtca gttacttgtt tgaccgcaaa ggatatatcg ctattgaacg
     6181 cgatgggttg actgttgatg aagagacgat gtttgaggat gtattagagg cgggggcaga
     6241 agatctgcaa acttctcctg aagtattcga gatttatact gcaccagaag attttgctgc
     6301 tgtacgagat gagttggaaa aagagttcac tttggcgcaa gcagaattga cgatgattcc
     6361 acaaacgacg attgatttaa atgaggaaca aaaagagcaa ttggaacgtc tagtagataa
     6421 gctagaagac gatgatgatg tttcagaagt ctttactgct gctgaaatgt aagtagcaca
     6481 ttgttagata ataaaaagaa agcacctgta ccaatcgtac gggtgctttc tttttatata
     6541 ttatttttgt tagaagacca atacaggcgt tcctttttcg accatcccga ataattcctt
     6601 cattacactt ggtggggtgt tgatgcagcc gtgtgaaccg cgggttttcc ataagtctcc
     6661 accgtattca ggttgccagt ctgaatcgtg gataccaaca cctgtccagt caatcggcat
     6721 ccagtagttt accggactct cgtaaggtgt tccatcgtca tttgttcctt ttaaagttgc
     6781 atcttcttct ttattccaaa catagaagac tccagcaggt gtaggtgtag ttggctttcc
     6841 ggaaacgata tctgtttcaa gggccacttt tccgtctttg taataccaca tatgctggtt
     6901 ctccagatca acttcgatat acgtatcttc gatcaatgga tgatcagcgg ttgtgccacc
     6961 ttgcacaatg ggtgaacgag taaaatcttg gcctgccaga atcgcttttt tcaatgcttc
     7021 tgtctcactg tctgtttgga tcgtccaact gtaagtgcca actgggaccg taacttctcc
     7081 tctttttgta cttttaaatt ttgtgtcatt cgtacttgtg ttatactttg tgcctaaatc
     7141 tgttacatat tgacgtactt gttctgtatc taaatcgact tttccatcat tgtaggtcag
     7201 ccaactcata atatcacttg atgggatctg gaaagtctca ccgtttatac tgtaagtagc
     7261 ttttacattt gcgatagcat tcatggatgc gagttgttct ttcagtgagc tgtcttcaga
     7321 tgttactttt ggtttttctt tgaattcagt caattcaatt gtatctttac cagagtttac
     7381 cgcacttttt aattgtttga ccgcagcatc gacgtcgatc gtatttccat tgacttcagg
     7441 cttgataacg aaagagtcac ctgctttttc gatcgttgca tcagtagtag gtgtccggtc
     7501 tttgttcaaa tcagtcagct tagttgtcaa tgtctgaacg gtagtgtcta gtttttggtg
     7561 gttttgactg acaggatcta tttcttgttt ttctgccgct gagacataag tcattcccca
     7621 agtccatccg ttttgttcat ttaggatcgg agaaagctcc gaagtgaaat catctttgta
     7681 gccgagctcg gattttggta cttcctgcca gacttttcca tcttctttaa tagccagttt
     7741 tttatcagaa taagcttctt ttaatttttc atttgctttt tcgactgtta gattgctgac
     7801 atttattcca tttatttctg tattcggcaa aaaatgcttt gtgtagtatg tactgcgata
     7861 ggtataaaag ctgatcagca ctaagagaac accaagaact gaaagcaaag caacatgttt
     7921 cgattttttt cgatgcgaag atcttgtcat atcgacaact ccttaagtaa aagtttaggg
     7981 tggaacggtc tcatgatagc ataatttaga ggaaaagacg aaggttatct ctactttatg
     8041 aaaaaattta gaaaccataa gggtgataaa ataaaaaaac ggaaaacacc gaatagatgt
     8101 tttccgtcat gttcaaagat tattcagcgt ctagtttagc aaaagcttca tctagaattt
     8161 cttttaattg cttagcagaa gcagccatac ggtcttgctc gctgtcactt aaaggaattt
     8221 cgattacttt ttggatacct tgacggttga taacagcagg tgcaccaatg tagatgtcgt
     8281 tcaatccata ttcgccattc atgaatactg ataaaggaag aacagcattt tcatcatcta
     8341 agatggcacg agtgatacgt gctaatgcta ctgcgatacc atagaaagta gcgccttttt
     8401 tctcgataat tgtgtaagca gcgtcacgta cgccaaagaa taggttaacc atagcttctt
     8461 catcgatttc tgggttgttt tttacccatt catagatttg caagccggca acatttgcat
     8521 gtgaccaaac tgggaattct gaatctccgt gttcacctaa gatgtaagca tggacgttac
     8581 gtgcatcaac gtcaactaat tccgcaagtg cttgacggaa acgagctgag tcaagagagg
     8641 tacctgaacc gattacgcgt tctttaggga aacctgagaa tttccaagtt gagtaagtca
     8701 aaatatctac tgggttagcg gcaactaaaa agataccgtt aaatcctgat tcaacgattt
     8761 gtgtgacaac atcgcggttg atttttaggt ttttgtttac taagtctaaa cgagtttcac
     8821 ctggtttttg tggtgcgcca gctgtaataa caactaagtc agcatcatgt gcgtctgcgt
     8881 aagtagcaga atagattttt ttaggtgaag taaatgccaa agcgtgagac aagtcaattg
     8941 cgtctccttc agttttagca gtattgatat ccacaatacc tacttcttga gcgatatttt
     9001 gagtaactag agcaaaggca tagctagaac ctacggcgcc gtctccaaca agaataactt
     9061 tttgatgatc ttttttttct gcagctgcag tcatttagta tcaatccttc cattgatttg
     9121 ttttttccca tgcttatgct aacatttctc aagccccatg tcacgctatt tggctataaa
     9181 atataacaga taacaaggtt gtattatatg taaaagctta catgtgcgtt atttcactat
     9241 ttttatatat caattatttc attaaacttt gaagaaattt ttaatttatt tatcatttat
     9301 taacgattct cttttagccg aaacgccttg ttttgtgtta tactatgcat tgacaagtat
     9361 aaaaaagaaa aaagctcgat ggactggacc gctaattagc agtccagcct tttgtgctgc
     9421 tttttagaat taggtggaaa acaaaaatga aaatgatcgt cgggttagga aacccaggta
     9481 ccaaatacca atatacaaaa cacaatatcg gctttatggt cgtggataaa attgcacgcg
     9541 agcatcaagc tactttcaaa aaaaatccgt tcgaagcaga agttgccgaa ttttttcata
     9601 atggagaaaa gatcctttta gtcaaaccac aaacattcat gaatgaatct ggtcgtgcag
     9661 tcggcccact aatgacttat tttgggattt atccagagga attagtcgta atctacgatg
     9721 atttggatct ggcagtggga aaaatccggc tccgtcaaaa gggaagtgcc ggcgggcata
     9781 acggtatcaa aagtattatt tcccatttga atacgaatgt ctttgatcga atcaaggtgg
     9841 gaatcggcag gccagaaggg aaaaaaacag tagtccagca tgtgcttagt cctttcagca
     9901 aagaaaatca gcctttgata gaagaaagca tgtgtcaatc tgttaaagca gtcgaatatc
     9961 tcatagaagg gcattcgttc gtggatgcga tgaatcgatt caattgagga aggaggaaaa
    10021 ggaatggata ttattcaatt agtcggaaaa gatccgcaag taaaagaatg ggtcagcaat
    10081 ttagaagaaa aaatgcctag acaactaatc acaggattag cgggctcagc gaaaacactt
    10141 ctatttgctc gagcgtttaa gaaaacaaat aaaaacatga ttattctgat gccaaatttg
    10201 tattatgcga atcagttagc agaagatctg caacatgtga ttccgcaaga tcagatccat
    10261 ttatttccag tggatgaagt gctttctgct gaaatggcct tttcttctcc agaagcgcga
    10321 ggcgagagag ttgcaacgct taacgcactt caagaaaaaa aagcaggtat ctatttgttg
    10381 ccagtcgcat ctttgcacaa acgactgccg aataagaaaa cttggctaca ggcgcaattg
    10441 aattggcata ttggtgatga gatagacgtg gaaaatcttc ctcgccaatt gactttgatg
    10501 ggctatgtac gagaagagat ggtcgcaaaa ccgggagaat tcagtattcg aggaagcatt
    10561 atcgatattt acccgttaac gacaacctat cctgttcgtg tagaactatt tgatgtagag
    10621 atagattcta tgcggtattt tgaagcagaa acacaacgct caatcgaaaa aacggaagaa
    10681 atctgggttt ccccaacgag tgaacaagtc tacggaccag aggatctaga acatggaata
    10741 gagcaaatgc aaaaaatgct agaaaaacgg cttgctgcga ccacagaaga agcagaccgc
    10801 gagtttttgc aagattactt cggtcagctg acttcaagct ggcaagcagg tgtcccgaca
    10861 gaagaagcaa aattctactc agatcttctt taccaagaat ctgtgacgat cttggattat
    10921 ttcccttcaa atagtttact agtcgttgat gattatcagc gaatcatgga aacaaatcgg
    10981 gaaatcgaaa gggaagcggc agaatggcag actcaaaaaa tcagcgaatt gcgggtgttt
    11041 tctgagcaga cattttcagc agatatacaa aaaatcgtgc agaaggaaaa gttcaccaca
    11101 acctttttct cactcttcca aaaagggatg ggaaatcttc gtttccaagc aatccataat
    11161 tttcaatatc ggacgatgca acagttcttc ggacaaatgc cattgctgaa aacagaaatg
    11221 gaccgttggc gcaagcaaaa acagactgtt gtgatcttta ttccgacaaa ggacagaata
    11281 cgcaaagcag aacaaatgct tcgagatgaa gacatattag tagtagaaac agaaaaaaat
    11341 gcattgatca gaggacaggt acaattagtt gaaggagctt tgcaggctgg ttttgaactt
    11401 cctcaagaaa aaatagtagc tatcacagaa aaagaaattt tccaaaaaac gacgaaaaaa
    11461 caaacgagac gtcaaacagt aacaaacgca gagcgattga aaagctataa tgagttaaaa
    11521 gcaggagatt acgttgtcca tgccaatcat ggtatcggga aatacattgg gatggaaacg
    11581 ctagaagtag atggcgttca ccaagactat atgactattt tgtatcaaaa cgacgacaaa
    11641 ctgtttattc ctgttactca actgaatctg atccaaaaat atgtcgcctc tgaagccaaa
    11701 gcgcctcgca tcaataagct aggcggaagt gaatggacga aaaccaagcg aaaagtctct
    11761 tcaaaaatcg aggatatcgc tgacgatcta atcaaattat atgccgcaag agagtcagaa
    11821 aaaggatatg cttttggacc tgatgacgcc taccaaaaag aattcgaaaa tgccttcccc
    11881 tactctgaaa ctgatgacca gttgagaagt gcagcggaga tcaaacggga tatggaaaaa
    11941 gaaaaaccaa tggatcgact actcgtaggt gatgttggtt atgggaaaac agaagtagcg
    12001 cttcgagcgg cctttaaagc gataaaggaa agcaaacaag tagccttttt agttccaaca
    12061 acgatcttgg cacagcagca ctatgaaacg atgctggaaa gatttgaagg tttccccgta
    12121 aatatcgggc tattaagtcg tttcagaaca agaaagcaac aaaaagaaac catcgaacaa
    12181 ctgcgtacag gtcaagttga tattgttgta ggaacccacc gaattttgtc aaaagatatt
    12241 gaatttagcg atttgggtct actgatcatt gacgaagaac aacgttttgg tgtaaaacat
    12301 aaggaacgat tgaaacagtt acgtgcacaa gtggatgttt tgactctgac tgcaacaccg
    12361 attccgagaa ctttgcatat gtcgatgtta ggcgtgcggg atctttcagt aatcgaaaca
    12421 ccgcctgcga atcgttatcc gatccaaacg tatgttatgg aaaaaaatcc tggggctatt
    12481 cgcgaagcaa tccatcgaga aatgggacga ggtggacaag tattctattt atataatcgg
    12541 gtagaaacga tcgaacaaaa agtagaagaa atccaagaac tcgttccgga agcacggatc
    12601 ggttatgctc atgggcaaat gacagaggcg caattagaaa atacgctttt tgaatttatt
    12661 gaaggtcaat atgatgttct tgtcactaca accatcattg aaacaggagt agatatcccg
    12721 aacgcaaata cgttgttcgt tgagaacgct gattatatgg gattatccac gttataccaa
    12781 ttgcgagggc gggtaggaag aagcagccgt gtcgcatatg cttactttat gtatgagcag
    12841 cagaagatcc ttaacgaagt cagtgaaaaa cgtcttcagg caatcaaaga ttttacagaa
    12901 ctgggatctg gattcaagat tgcgatgcgg gatctttcca ttcgcggtgc aggtaatctc
    12961 ttaggtgctc agcagcatgg atttattgat gcagtcggtt tcgatatgta ttctcaaatg
    13021 ctaagcgaag ctgtttctcg taaacaagga aagaatagtc aagtggagaa gaccactgtt
    13081 gagatcgacc tcggtgtcga tgcgtatcta cctgaaacat acgtggcaga tcaacggcag
    13141 aaaatcgaga tctataaacg aattcgtgaa cttgattcgc aagaaatgct agatgaacta
    13201 gaagatgatc tgctcgaccg ttttggagaa tatccagaag aagtagcaca tctcttgaca
    13261 attgggcaga tcaaaatgga tggcgatcga gcgctcctag aaacgataag gaaacaggag
    13321 gcgcatatag tctttactct aagtaaagtt ggaacaaaac gctatagtgt ggaacagtta
    13381 ttcgaagcac tgacagcgac aaaactaaaa gccagtctag gcgttgaaaa agaacagatg
    13441 gtgatacgct tgacgatccc caaccaaatg gaagaagcag tatggcttca ggaaatccaa
    13501 aagtttgtca aagcgttgcg tgagcaaaaa tatctgtcag cgtaggaagg agcaatgatt
    13561 tggatcataa agaaatgcgg cgcgtcatgc aaggagcatt tgtattgacg attgcttctt
    13621 tcatagcaaa agtattaagt gccctttacc gaattcccct ccaaaactta gtcggcgacg
    13681 aaggttttta tgtttatcag caagtttatc caatttatgg gatagcaatg actttagctt
    13741 tatccggtct tccgcagttc atatcaaagt acgttgcaga aagaaagaat cctttcgagc
    13801 gaaagcaagc tttgcaaaat ctttatccct tagtattttg gacaggtgtc tttttgtggt
    13861 tgtttgtctt tgcatttagc agctggattg cttggatgat gggagacata caattgaaac
    13921 cgctgatcca agtagtttcc tttacctttt tgctgatgcc tggtctatct ttttatcgag
    13981 ggaatttcca aggaagattc ttgatggaac caactgctgt ttctcaagta gttgagcagt
    14041 tcgttcgtgt ttttgtgatt gtctgtagtg cgtttgcttt tagaaagctt ggatggacgg
    14101 tgtaccaaac agggacggca gcaatgagtg gagctgtttt tggcggttta tgcgcatatg
    14161 ggatccttca ctactatgaa caaaagattc atggaggagc gttaagcttc cgacggtttc
    14221 cattgttcaa acagccgtca aagcccttga tccggcgttt tctggtagaa ggcggactgg
    14281 tttcgattta tagtggattg cttatctttt tccagctgat cgactctttc tttgtaaaaa
    14341 atgctttgga agtttacgga ctgtcagaat acggtgcgaa gatagcaaaa ggggtctatg
    14401 atcgaggtca gcctttagta caattaggtt tagtgattgc cactgcatta agtgcgacat
    14461 tcctgcctgc gttgactcgt catttgacca accggatcta tcgtcagttt ttacagacag
    14521 ccaaaatcta tcttagattg acgaccgctt tagcattagc tgcgtctttg ggattagcct
    14581 tgctgctccc ttatatcaat tatgcgttgt ttaaagatta tgcgggaaat gcagcgcttg
    14641 tattatttgt tttttccatt gctttcactg cagtcatcca agcttatcaa agtattgctc
    14701 aaagcaagaa ctcgtttcgc ccttctttaa aaggcgcagg atggggacta ttggtaaaag
    14761 gattgactac ttcttttctg accggtttat taggaacagc aggtgcaagc ctgtctaccc
    14821 ttttaggatt aggtgtgacg ctttggtatt ttgttcgaat tgaaacaaaa gagctaaatg
    14881 ctttttggaa agaacggcat tttggacgga agttgatgtt cagccttctt tgtatgacag
    14941 tagtactgct gctgtattat ggtgtattga atatagtatt tggtcctgta actcatcgtt
    15001 ccactgcttt ttttgtcagc gtgataggag ttgggatcgg ggcttctgtg ttcatttatg
    15061 cagtgattcg tcttcgtctg tttacagtac gagaatggct aatgttgcct tttggtaaga
    15121 aaatcctgcg attaaataaa aaaattcgcc gattgtaaaa ttaagctaga caactaaaaa
    15181 agtcatttgt gctacactca aaatagttcc gaccaaagaa ttataaggag tgagtacaaa
    15241 tgacctatac ccatcttaca acggatgaac ttgtaataat agagtcttat ttcaaaatga
    15301 atcaatctgt tgctaaaact gcccattgct tgaatcgttc aagacaaacg atccataaag
    15361 tatacctgtt cttcaagcaa ggaaaatcag ccttagagta
//
LOCUS       DAZUMK010000058        15380 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000058 DAZUMK010000000
VERSION     DAZUMK010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.059"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            73..2133
                     /locus_tag="ACG808_001695"
     CDS             73..2133
                     /locus_tag="ACG808_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFS5047321.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2216..2785
                     /locus_tag="ACG808_001696"
     CDS             2216..2785
                     /locus_tag="ACG808_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5047322.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(2960..3766)
                     /locus_tag="ACG808_001697"
     CDS             complement(2960..3766)
                     /locus_tag="ACG808_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047323.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3904..5418
                     /locus_tag="ACG808_001698"
     CDS             3904..5418
                     /locus_tag="ACG808_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFS5047324.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5821..6804)
                     /locus_tag="ACG808_001699"
     CDS             complement(5821..6804)
                     /locus_tag="ACG808_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFS5047325.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6809..7456)
                     /locus_tag="ACG808_001700"
     CDS             complement(6809..7456)
                     /locus_tag="ACG808_001700"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFS5047326.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7541..8173)
                     /locus_tag="ACG808_001701"
     CDS             complement(7541..8173)
                     /locus_tag="ACG808_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS5047327.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8189..8740)
                     /gene="coaC"
                     /locus_tag="ACG808_001702"
     CDS             complement(8189..8740)
                     /gene="coaC"
                     /locus_tag="ACG808_001702"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFS5047328.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8737..9474)
                     /locus_tag="ACG808_001703"
     CDS             complement(8737..9474)
                     /locus_tag="ACG808_001703"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFS5047329.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9471..10460)
                     /locus_tag="ACG808_001704"
     CDS             complement(9471..10460)
                     /locus_tag="ACG808_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFS5047330.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10718..11260
                     /locus_tag="ACG808_001705"
     CDS             10718..11260
                     /locus_tag="ACG808_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFS5047331.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11277..12362
                     /locus_tag="ACG808_001706"
     CDS             11277..12362
                     /locus_tag="ACG808_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5047332.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12374..13180
                     /locus_tag="ACG808_001707"
     CDS             12374..13180
                     /locus_tag="ACG808_001707"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5047333.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13181..14008
                     /locus_tag="ACG808_001708"
     CDS             13181..14008
                     /locus_tag="ACG808_001708"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5047334.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14005..15078
                     /locus_tag="ACG808_001709"
     CDS             14005..15078
                     /locus_tag="ACG808_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5047335.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
BASE COUNT         4944 a         2778 c         3136 g         4522 t
ORIGIN      
        1 tttatataat gaaatcatct tatgaaaatt actacttcat gtttagacaa gagtagggag
       61 gactaagaat aaatgtgttc aaaaagacgt tcaaaacaac taaataaaca cgcttgtcgt
      121 ttagataagg aacggtcgtg ttctgaaaaa cactacgtta agtttatgat gcgcacgatg
      181 ctgatcagtt tatctgtagg tgtctatatg catgtacccg ttcaggcaac gacacaaacc
      241 atcaaggtcg tacgtacctc tgtttctgag accactgatt cccctatcac tattactcat
      301 actttcactg atgcaaatgg aaaacagcta cttcctgcat tgacttccta taataaagtc
      361 agttatcctc caacgataaa ccactactgg ttaattccta acaaaacatt atggacatcg
      421 gaaaccggaa gcagtgtgcc gctttctatc ttttccgata tgactgatcc tacaaaaggt
      481 gatcagttaa aacagatcac tgatattttg aatcatgctt tgacagatat aaaggacggt
      541 ggaagcgcac aagtttctta tgtatatgaa aaagattatt cacagtttga aagaaagaaa
      601 atcgatgttc tggcagggac aaaagtttcg atatctgatc tggtagaaag gctgaagaac
      661 gcagatggaa cacaaggaga tatgtccaaa gtaatttcaa acaatgggaa tatcgtggat
      721 acgactaata caggaacatc tactatttcc ttgtcctatt ttgatccaat tgctttaaaa
      781 acaatgactg catctgctgt gatccatgtt cgtgctgatc aaacagctat caaaggaaaa
      841 gatcttcact taagaacagg agagagttac gcccctaaag atcttgttga atatgtaaca
      901 aaaagcgatg gaacttcagg aaataaagaa gaaatcaaaa tgaacgggac agtagatact
      961 acaactccgg gagaatatcc cgttgtatta acttatatag actcgaaaac atcaaatgtg
     1021 gcagaaacaa cagctgtagt ttatgtagaa gcatctgctg cttcatcgac tgtcatcact
     1081 cattttatag atagtcaaac aaataaagaa cttcaagcag aaacacatat gagtagtgta
     1141 aatcgtattc ctgatttgcg cctacctgct gaattttctg atcgagtgcc aaatatagat
     1201 ttatcgactt atgaacaaaa tgggaaagtc acttctattt ctgattggat gcaagcaaac
     1261 ggattagaca gaaaaagtca ctggtcggag atccttgaag tacttcactc agaactgatg
     1321 aataatagga agcaatcagg aggggaaatt acctttactt atgtttatat accagacaat
     1381 cgttcattag aaggtcatga tcttattgtt catccaaacg aaaaaataaa caaagaagag
     1441 ttgatccaat cagcaacaaa ttccttaggt aaacccgtac cgttaaagga tgtatcggtt
     1501 acaatcaatg aagtaccatt ggaggatggt tatttgacca aacaaacagg tgattttacg
     1561 attatgtata gttatcatga cccgtattca ctccaagaac tccatgctca gtcactatta
     1621 catgttttac cattaaacaa acagatagat caaaaagaac aaaaaagaca agaagaaagc
     1681 ttgtcagaaa aaaaacaaca aatggctcat gcagtttctc caacagggaa agtaaaacaa
     1741 acaagaagcc tagagttagc aagaatatct agaaaaatct cagaatctaa taaaacgata
     1801 aggcaagaag ctcatttggc cccacaagtt tctggattgg aaaatgaaat tttagagcct
     1861 aaaataaaac aacaatcaga aaaagaattt gttgctaaac agtcggaaga tcagaaggaa
     1921 tcagatgacc agaaggaaac gaagaaaaaa cgcttgtcaa ctattgattc caccgatgag
     1981 catctgcagc cgcaagtacc acttcctagt ccgggaacac ccgcacctgg tggaggtatt
     2041 ccttcttctg ctttaggaga tttcatgcga ggtatggcag gaacatgggt atacgctaat
     2101 cgaaatgaat ttgacgcaga tgactggttc tgatttatat ctctatctgt tatagtcaaa
     2161 taagtagctg agtcatatgt tatttggcat aatggagacg aggagggata gaaaaatgtt
     2221 agataaaacg ttaccttacg cagaaatctg gatgacaaga ccacttcaac aaaatgttcc
     2281 aatcacgcct ttagcggaag gttttgaatt tcgattttat caaaaagaag atgaatctgc
     2341 ttgggcggag attgagactt ccgttgggga atttcaaacg acagatgaag cacttgagta
     2401 ttttcaacaa aactttgctc cttatcctga agaacttgaa agaaggatgt tttttgtaga
     2461 gacaaaaaaa ggagaaaaag tagcaacctg taccgcttgg cgaaaaaaat caagagagca
     2521 agtgtatccg gtttttcatt ggttagcagt gaaaccagat taccaaggaa aagggctcgc
     2581 aaaagcattg actgccagag tcttgcaaga atttcctacc ctccatacgg acggaccaat
     2641 ctatcttcac acacagacat ggagccatca ggcgattgct ctttataaaa aaatgggttt
     2701 tacttttatt gctgaaaatc tagacggcac acctaatcca gattatgaaa aagttatgca
     2761 ggttttaagt aaattagata aataacgaaa tggtgaaata agtcctcagt attgggataa
     2821 aaaacgaaat agtcattcct catatcttac ttttttgagt taatactatc ggataactaa
     2881 tggcatgcca tacattcaaa aaagaagggg cagtgacaaa agtattgtca ctgccccttc
     2941 tcttgttttt tagcatgttt tatcgtttga tttttttagg aataaaattc ttgatgaacg
     3001 taaatttttg gtaatcgttt cgactataga ggtaaccaaa tacactgaaa acaactgctc
     3061 caataaaatt aacaaataaa tccttcatag tatccatgat tcccacgtct aagtaaccat
     3121 tttggatgat tgttttatct gttttaccat ctgcattttg actttcaatg attgtccgag
     3181 tgatatctgg aacatgatag acagtgtttc cgtctttact taactccaca ctattgattt
     3241 gctggatgat cgtatctttt tgcatatcga attgcagtaa tttatctgct gaaaattcca
     3301 caaactccca cataacacca actgtcatcg aaaaacagaa ggaaacaact gctagtaata
     3361 aaggagtcaa gttcatggtt tttgtatttt tattgagcaa ataaacaagg gaaaagccga
     3421 cacccccagc taaaaatccg ctgaatgcat gtagaaaagt atcccacatt gaaaaatagc
     3481 cataaaaatc acttaactct cctaaaactg ttgaactaaa aataaaagat aacaaaatga
     3541 tttctagcat atttggaatc tgtatcttta atatcttttc cagaaaataa gggatttcaa
     3601 ataaaattaa tgtcagtaat aaaagcattg tatttcccca gtgttggttc cagacctgat
     3661 ccactagcat aataacgacg atgatacgta ataaaatata gattccgttt gtgattctac
     3721 ctgcagtttt tgtactgttc gtaaaaaagg ttttcaaatg attcataaaa taactccttc
     3781 atgtatatcc ttaattatac taagaagcgt gtgtttacca aagctatatg acttaaagaa
     3841 aaacagagtt gatattgtga aaaaaatcac ttaatatagt ataaggagaa aagggggata
     3901 caaatgaaaa aattagttgc tggtttatta acagccgttg cacttcttgc tatgagcgca
     3961 tgcggcgcag atcagaaaaa tacagcgcag acaagttcta gtacgaagga atctgccgaa
     4021 gtatcatcag gcgcttctga acaagtatat acagatccaa aagaaatgaa agaatcttat
     4081 gatgtcgtga ttattggagc tggtggtgca gggatggcag cagctatcga agcaaaagat
     4141 gctgggttga atccagtaat tttggaaaag atgcctgttg ccggaggaaa cacatctaaa
     4201 tcatcaagcg gtatgaatgc ttcagaaaca aaatttcaaa aagaagaagg aatcaaagac
     4261 tcgaatgatg ccttctatga agaaacgcta aaaggtggtg gaggaacgaa tgataaagaa
     4321 atgcttcgat actttgtcga tcactctgct gatgcggtcg attggctaga tcaaaacggt
     4381 atcaaactgg acaacttaac gatcactgga gggatgagtg aaaagaggac acatcgtccg
     4441 tctgatggct cggctattgg tggatactta gtggaggggt tattgaataa cgtccatgag
     4501 cgggaaatcc ctatttttgt caacgcagat gtgacagaca ttaagaaaaa tggggaaacg
     4561 gtcaatgaag tgactgtaca tgttcaagga gaagaaccaa aagaggtcac cggaaaagca
     4621 atcgtagtta ccacaggcgg ttttggtgcc agcaaagaat ttattgaaaa atatcgtccc
     4681 gatttagctg attatattac gacaaatcaa gaaggatcaa ctggagatgg tatcaccatg
     4741 attgaaaaat taggtggaca aaccgttgat atggataaaa tacaaattca tccaactgtg
     4801 cagcaggatg agggggtctt gattggtgag gttgttcgcg gagaaggagc aatcttggtc
     4861 gatcaagagg ggaaaagatt tgtcaatgaa atggatacaa gggataaagt atctgcagca
     4921 attacagcat tgcctgaaaa atcagcttat ttgatttttg accaaggcgt gcgtgatcgt
     4981 gccacagcta ttgaatttta tgaccaaaaa ggatatgtca caactggaaa gacaatggaa
     5041 gaattagcag aaaaaatcga tttaccaaaa gaaacactag caaaaacagt tgatggatgg
     5101 aatacggatg ttgcggagaa aaaagatgct caattcaacc gtgccacagc catggaacat
     5161 gacttatcgt ctccgaatta ttatgcgatc aaaatcgcac caggaatcca ttacacgatg
     5221 ggcggtgtga aaatcaatac gaacactgaa gtattagata aagaaaacca gccaattagg
     5281 gggttatatg ctgctggtga agtcacaggt ggcttacatg gaaacaaccg gatcggtgga
     5341 aattcagttg cggagatcat tatttttggg cgacaagcgg gcatacaagc agctcgattt
     5401 gcttcggaaa atgaatgaaa tagaacgtaa ataaaaagag gttgtgaaat cagcgttttg
     5461 agctaatacc gagggtcagc tgattgaaca ctgtttagtg aagaggttgt gaaaaagctg
     5521 ccctgcatca agtaataaga acagccaagt aaaaacagct tcttatgttt ttaacttggt
     5581 tggacttatt accgcagatg caagcttttg aacaccgttt attcggaaaa agagcgtggg
     5641 acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaagcagaa
     5701 gaaattcctt cggaaataag ctgaaattca caaaaatttg aaaagcaatt ttcgtgaatt
     5761 tcttcttatt tcttggagtt aaacgcttct gtcccagcct cttttttgat cagcatgatt
     5821 ttattcagtt atcttcaaaa tgatttttcc gatgtttttg tttgcttcca tatattcatg
     5881 cgcttgtttg actgcgctga attggaatgt acggtcaatg attggccgaa tcttgccatt
     5941 ttctagaaaa ggcccagctt tttgcataaa agtttgtgcg agctctgctt tatactgatc
     6001 acttctaggt gtcaataatg taccaagcag ttggacacgt ttcccaatca atacaccaag
     6061 gtctatctca ggcacgatgg tcccacctag cataccaatc aagacccaac gtccatcaac
     6121 ggctatagaa gctaaattct tttcccagta agaagcgccg atgaaatcca aaataacatc
     6181 gactccccga ccttcagtga tttttcgtac ctcttcttga aagtcttgtt ttttatagtt
     6241 gatcaattca tctgcaccaa gttctttaca aaaggcgagt ttttcttctg atcctgcagt
     6301 aacgatgatt ttcgcgtgag aaagctgtct agctaattgg atcgctgctg ttcccacacc
     6361 acttgctcct gcatggatca agacagtttc gccttcttgt aattttccaa gccagtaaag
     6421 tgtttgataa gcagtcaaaa aaacttctga gattccagca gcagaaacat agtctagcgt
     6481 atcagggaga aggattgcac gatcggcagg catcacagca tattcggcat aaccacctag
     6541 attgactaat ccgtatactt ttgtcccagg agtaaaagtg gagatatcac ttttattctc
     6601 aactacctct ccagctactt cgactcccaa aatagggtag ggaggtttca aagagactga
     6661 ttctcttttc ataatatctg ttcgattgac agctgctgta tggactttga tcagtaattc
     6721 gccttgtttt ggtttaggaa caggcacttc aatttgttct aatacatcta cagaaccagg
     6781 atgcttgatt gaaatggcac gcatggatct attcctcctt tgcccattct tggaatgctg
     6841 tattcaactc tttgctacct tcagaaagta ctagagtcgt acgagaaata tccgtttctt
     6901 ccactagagc ggcaagtaca gcagcaactg tagagcgagt gatcgttttt gatgccttct
     6961 cattaggctt agttaattga atggaagtaa cttcttcatc gttagtcaat gtgactggtt
     7021 gaagaatgac aaaatctaag ttagttcgtt tcagccattc atctgcataa tgtttagtaa
     7081 tataataatc tgtcatggac tcaggccatt ttgctggttc atctgcataa acagcactga
     7141 ccatcagata gcgagagaca tgcgcatttt cagctgcgat cactgttttg attgcgccat
     7201 ccaagtctac ttgcagaaga ttctttcctt gagagccggc agcaaaaatc aatacatcga
     7261 tgcctttgaa tgcttcggtc attttttcca cggaccaagt taaatcaaaa gagacatacg
     7321 aaacattctc tttttttact aatgtttggg aagcaacatc tctcacccct gcaaaaattt
     7381 catgggaact tggagctaaa tcttttatca ggtgtcggcc aatttgtccg tttgcaccta
     7441 caacaaaaat tttcatttgg atcatccttt cgacgtcaaa tctttccatt caatagtaac
     7501 accaaaagag aaaaaaatca gagatcggct acgatgacta tcagttagta agagaagggt
     7561 gcttgaagta tcttaacaag acattgcata ctgctgcagt cgcaagacct gcgacgattc
     7621 cttcaggtat accacttgta aaaataaccg ctagaatagc tgaataaaca gcatttaaat
     7681 ttgttccgat gactttggca taatcttgct ggaataaaaa gtagatcata ttcatcacta
     7741 aaatcgtatt ggtcaatgaa ccggaaagtc cagctaagaa taaagaaacg gaatctgctt
     7801 tttgtttcat tagtttgcgc agacctttat aaacgaaata aggaaccacc ccaatcaaga
     7861 tgcgaggaat aaacgcgatc agcaaggctt tccagcttcc atgttctgtt ccgatgactg
     7921 ggataaacgg agaaaagaca aaagacaaag gtgtttggat aactgtgcta cggatcatgc
     7981 tgatcagtcc aaatgtaccg ccaagaaaag cacctagtct tggtcctaaa acgatcgaag
     8041 caatgattac aggaatatgc atggttgttg cattgattgg accgataggg ataaaaccta
     8101 aaggcgtaac agctaataaa atcaaaatag ctaaaaacat cgcagttaat gtaaagtttt
     8161 ttgtattttt cataagtact ccttttttct aagcatttct ttcattaaga gtcagcatga
     8221 cctgttcgat gatcgtctga atatcagcta aggcaccttt tccaaaatct ccacaagcta
     8281 aaagagattc tcttggttca atttcctgat agccgacaga tctcaatgtt tccagattac
     8341 gctgagtgat cgggttttga tacatattcg tgttcatggc aggggcaatc aattttggtg
     8401 ttcccactgg aagcgccatt gcgactgttg aaagaagatc gtctgcaatg ccgtttgcta
     8461 gcttgccgat aatgtttgct gtagcaggcg cgatgagaaa caagtcagct tgttttgcta
     8521 gttcgatatg attgattacc tgcggatttg attcttgcat gacgtctgta tggactgcgc
     8581 gcttcgacag agattgcaag gttaatggcg tgataaaatg tgtgctgctt ttagacataa
     8641 tcacatctac ctgggcacct aatttgacga attgacttgt aagatcagcg cttttgtaag
     8701 cagaaatact tccagtaacg cctagtaaga tctttttcat tttgattcct ccagtctaat
     8761 tttctcagta atcaatgcag caatctcaga tttactttga gcttcttcca ctgttccatc
     8821 tttagataag agatagccgt ggtgtgtctc gccatggatt tccgtcaaat catttgcgaa
     8881 aataaaatca gttttgttat ttatcagact attttttgcg actgccagaa gagattcctt
     8941 tggtacatct actaatagtt tgaaaccaat gatttttgct tttggctgcc attcacgtag
     9001 ataagaaatg atttttggtg ttttcttcaa aacgagcaca agatgctcgg tgttagaaga
     9061 gatctttgaa acagtggtat ctgtctgttc attttccgca aaccaattgt ccagttcttc
     9121 ttgcgttgaa gaagtgggta ggttttttgc cagctgttct tctgaaaaac taaaagcagg
     9181 agtaaaatca ctgactgcca tactgtggat aatcgcatcg tagtgttctt ttttcgttag
     9241 ttcttctaat tgtaaaaata agtccagtgc agattcgatc gtataaatcg ttatattgga
     9301 gtgtcttttc ggtttcagtg cttgtttcgt agtcacgtaa tcgattgtgg tatccggatt
     9361 ggcagctaaa taatcagcta atgcttgccc taatcgtcct gtcgaatgat ttgtaatagc
     9421 acgtacttga tcgatttttt cagtcgttcc tccagctgtg attagaatct tcatttatgg
     9481 tcacctacat ttactagtgt tcttccttgg tgtgttccag caagtagttt agatgctgtt
     9541 tcaggcaatt gatccagagt aatctcttgg acgaccagtt catctgcaat ctgcaaatcc
     9601 gccatctgtt gccataaaga aagacgttga tctattggta cattgacaga atcaattcca
     9661 atcaactgga tgtttcgcag aataaaaggc aagacagtcg cgtttagttt gattccccca
     9721 gcattgccac ataaagctac tgcaccgtta taagagatca atggaagaag actgcttagc
     9781 tgctcgccac ctactgtgtc gatgacatag tcgatttgct gttttcctaa agcttttgtt
     9841 gtctctggca gaaattcatc aggtcgtacg acttgcaaag cacctagact ttttagccat
     9901 gtgacagact cttcttttct ggaaaaggca atgattgact ggaatcctag tttatgtaac
     9961 agggctatcg ctgtactgcc tacaccgcca gaagcaccag taacgacaac ttttttatcg
    10021 gaggtcatac cttgacggat taaagcgttc acacacaaca tagcagtaaa ccctgctgta
    10081 ccaaggatca tcgcttgacg cagcgtcata ttttttggta gaggaactaa ccagtctcct
    10141 ggtacttttt gatattggct ataaccgcct ggatgggtga cgccaagccc aaaaccagta
    10201 gcaagtacaa gatcttcttt tgaaaaacgt ggattgttac tttggacgac agttccggat
    10261 aaatcaatcc ctggagtcat cggatatgtt cgtatcacct gaccagactc gctcattgcc
    10321 aatgcatctt tataattgac atcagaatag gcaattttta ccaagacttc attttcagcc
    10381 aactcagtaa atggcagatc actgattgct gtatggaaaa caggttcctt tattacctga
    10441 aaagatcgaa aagcgttcat tcgtgagaca ctccttttaa aattttcttt tatcatacca
    10501 taaagcctac tcttttattc ttgttcttcc gtaaatttag acaaaaatta ggtgaaaaat
    10561 aaagaatcaa acaactagaa tttattccaa aagataaaga aatattcagc aaaaaaaatt
    10621 ttacaaaggg ttgccttttt caataaattt cgtatactaa tacgagtggt agtaaacagg
    10681 aagtttacta ttagaaaact tcgattcgga ggaaattatg gaaattggtg agaagttaag
    10741 aaatttgcgc atccaaaaga atttgacaca agaagaacta ggcgaacgca cagatttgtc
    10801 aaaagggtat atttcccagt tggaacgaga tttaagttca ccatcaatgg aaacattctt
    10861 ttctattctt gaagtcttag gtgtgactcc agaagaattt tttcatcaag aagcagcgaa
    10921 tctgcaagtg gtttattcaa aagaagatca tacggtttat ttcgatgaag aaaacgggta
    10981 tgaattggaa tggctcgtgg ctgattcgaa tgaaaaagaa atggagcctg tcttgctgac
    11041 ttttgaaggt gcaggagaat ataagacgtt tgaaccttca ttatctgaaa cgtttatttt
    11101 tgtattagaa ggggaattag agttaagctt aggagagacg gtctatgtgg caaaagcagg
    11161 acagtcgatc tattatcaag cgacaaaaca acatcaatta aggaatcatc tcaaaaaacg
    11221 aacaaaagta ttgattgttg caacgcaatc atatttgtaa tatagggggt aaaaacgtgg
    11281 ggaaaccaat catttcattt gatcaagtag tgaaacgcta tgatgatgaa acagtattaa
    11341 aaaaagtaag ttttgagatc gaacaaggaa aattttatac actgcttggt ccttccggct
    11401 gtggtaaaac aacgatccta cgtattatag ctggattcac cgaagctaca gaaggggaca
    11461 tctattttga agggaaacgg ttgaatgatt tgccagctaa taagcgacaa gtaaatacag
    11521 tcttccaaga ttatgcactt tttcctcata tgaatgtttt tgataacgtt gcttttggat
    11581 tgaagatcaa gaaaatgtcc aaatcagata tcgaaaagaa ggtaaaagat gctttgcgta
    11641 tggtccagct tcctggatac gaaaaacggg agattagtga aatgtctggt ggtcaaagac
    11701 agcgtgtagc gattgcacga gcaattgtaa atgaaccgaa agtattgcta ttagatgaac
    11761 cgttatcggc gcttgatttg aaactacgta cagatatgca gtatgaactg cgggatctgc
    11821 aacaacgatt aggaatcacg tttatttttg ttactcatga ccaagaagaa gcgttggcaa
    11881 tgagtgatga gatttttgtc atgaataaag ggaaaatcgt ccaaagtggt acacccgtag
    11941 atatttatga tgagccaatc aatcatttcg ttgctgattt tgttggggag agcaatatcg
    12001 tggacggtgt aatgatcgaa gataatctag ttgagtttgt tggtaagcaa tttgaatgtg
    12061 tcgatggcgg gatgcgtcta aatgaaccag ttgaagttgt tttacgccca gaggatttga
    12121 cgatcacgac accagataaa ggaaaactag tcgtaacggt cgatactcag ctgtttcgtg
    12181 gtgtccatta tgaaatcatc tgttatgatg aacagcaaaa tgaatggatg gtccattcaa
    12241 cgaaaaaagc taaagaagga agcaaagtag gattggcctt tgagccagaa gatatccatg
    12301 tcatgcgttt caacgagtct gaagaagaat tcgatgcacg tcttgacagc tacgaagaat
    12361 aggggggaca agaatgaaaa caatgcgcag gatctattcc gtcccttata ttttgtggct
    12421 cggtttattc gtgatcgcgc ctgtattaat gatcatttat caatcttttt ttgatatgaa
    12481 tgggcaattc acgctgaata attatctatc ttattttacg tcggggacat cgctttctat
    12541 gacgatcaat tctgtatggt atgcattttt gatcacgttt ttcacgctga tcatcagtta
    12601 cccgactgct tattttttaa caaaactgaa gcacaagcaa ttatggctga tgctgattat
    12661 tttgcctaca tgggtgaatc tgctgcttaa agcttacgca tttattggga ttttcagtgt
    12721 gcacggcagc gtgaatcatt tctttgattt tatcgggatc gggccgaaac agatattatt
    12781 cacagacttc agttttctgt tcgtggcaac gtatatcgag attccattta tgattatgcc
    12841 aattttcaat gcattggaag agatgaatcc ttctttgatc agtgctagta gagatttagg
    12901 cgcgaatagc tttgagacct tccgacgagt gatttttccg ttatcattaa atggtgtgaa
    12961 aagtggtgtc caagcagtat ttattccttc tctttcttta ttcatgctga ctcgtctgat
    13021 tggcggaaat cgggtgatca ctttaggaac ggctatcgaa cagcatttct tggttacaca
    13081 aaactggggg atgggttcta caatcggtgt gattttaatc attgctatgt tcatcgtgat
    13141 gctcctgaca ggcgagaaga aaaaaggagg tcgctcataa atgaaaaaat ttaaatggtc
    13201 gtatctttat ttagggatcg tctttttatt cttatatgca ccgatcttct atctgatttt
    13261 ttattccttc aatgaaggag gaacgatgaa cagctttaca ggatttactt gggaaaatta
    13321 tgaagctgtt tttgaagata cacgtttgat cattatcgta ttgaatacgt tcatgctggc
    13381 tttcttatct gcattgatcg ccacgatcat cgggacattt ggggctatgg gaatctatta
    13441 cacgaaaaaa cgacgtgcac ggacagcgtt gttgagcatg aataacatct tattagtctc
    13501 tcctgatgtt attatcggtg ctagtttctt gattttcttt acactactag gattcggatt
    13561 aggttttcag agtgtattgc tttcccatat tgctttcagc attccaatcg tcgtcttgat
    13621 ggtcttgcca aaattgcagg aaatgaacga ttcaatggtc gatgcggcta gagaccttgg
    13681 tgctaataat ttccaagtag tcaaaaatat cattttgcct tatctttctc caggaatcat
    13741 agctggatat tttatggcat ttacctattc tcttgatgat tttgcggtta catttttcgt
    13801 cacaggaaat ggctttacga cgctctcagt agagatctat tctcgtgcaa gacaaggaat
    13861 cagtttagaa atcaatgcgc taagtgcgtt agttttcttg ttctccatga tattggtcat
    13921 tggatactac tttattagta aagaaaaccg tccaaaacgg ctgaagaaaa acagaagagt
    13981 acgcgagggg gtggcacggc tgcgatgaaa aaattacaat ctctgttttt aggtattctc
    14041 atcattattg cggtgctgct gcttggtgtt cgtcaacttg aaaaaacaag tggcatgagt
    14101 ggagcaaagg tacttaccat ctataactgg ggagattata tcgatcctag tttgatcacg
    14161 aaatttgaaa aagaatatgg atacaaagtg aattatgaaa catttgattc taatgaagcg
    14221 atgttcacaa aaatccagca aggtgggaca aactacgaca tcgctatacc aagtgaatat
    14281 atgatccaaa aaatgatcaa agaaaaacta gtacttccat tagatcattc gaagatcaaa
    14341 ggattaaata atattgatcc acgattttta gacttggatt ttgatccaga taatcagtat
    14401 tcgattccat atttttgggg aactttaggg atcgtatata acgataaatt ctttgatgca
    14461 aatcaaatta agcattggga tgatttgtgg aagccggaat taaaagacag tttgatgctg
    14521 attgacggtg cacgagaagt tatggggctt tctttgaata gtttaggcta ttctttgaat
    14581 agtaagaata tggcacagct gacagaagca gcaaataaat tgaatcattt aacaccaaat
    14641 gtcaaagcaa tcgtggcaga tgagatcaaa atgtatatga tcaatgaaga agcctccgtg
    14701 gcagtgacat tttctggcga agctgccgat atgatgtggg aaaatgaaca tttacattat
    14761 gtgatcccac cagaaggatc caatctttgg tttgataata tcgttatgcc aaaaaccgtg
    14821 aaaaataaag aaggtgccta tgatttcatc aactttatgc tagaaccgga aaatgcggca
    14881 caaaatgcag agtatattgg ttattctaca ccaaatgaag aagcaaaagc gttattgcca
    14941 aaagacattt cgtcggacga acaattctat ccatctgatg acacgatctc ccatcttgaa
    15001 gtctacaaag atctaggatc gaaataccta ggcatctata atgacctatt cttagaattc
    15061 aagatgtata ggagataatg gcaaaactca gtaagaatgg ggacttatca acgttctaaa
    15121 gagctaaaaa tcgtcctttt tctatctgag ttttccaatc ttctaccaaa agcggatgaa
    15181 acgccgcttt ttttgtgtta ttttattttt tctaccacgt ttttctacca gagaaaagtg
    15241 gacaaaacca ttgtaaaata gggcccgaga cgggctagta aatctaagat tgaaaaagtt
    15301 acagaaggat aaagtaattg gaaactatgc aaatatactt aacccttctg taatctatta
    15361 ataaaactaa tatatgaatc
//
LOCUS       DAZUMK010000059        14771 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000059 DAZUMK010000000
VERSION     DAZUMK010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.060"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(8..1270)
                     /locus_tag="ACG808_001710"
     CDS             complement(8..1270)
                     /locus_tag="ACG808_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5047336.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            complement(1263..2162)
                     /locus_tag="ACG808_001711"
     CDS             complement(1263..2162)
                     /locus_tag="ACG808_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5047337.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            complement(2307..4085)
                     /gene="uvrC"
                     /locus_tag="ACG808_001712"
     CDS             complement(2307..4085)
                     /gene="uvrC"
                     /locus_tag="ACG808_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFS5047338.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            complement(4267..4581)
                     /gene="trxA"
                     /locus_tag="ACG808_001713"
     CDS             complement(4267..4581)
                     /gene="trxA"
                     /locus_tag="ACG808_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFS5047339.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            complement(4668..7028)
                     /locus_tag="ACG808_001714"
     CDS             complement(4668..7028)
                     /locus_tag="ACG808_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFS5047340.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            complement(7143..7691)
                     /locus_tag="ACG808_001715"
     CDS             complement(7143..7691)
                     /locus_tag="ACG808_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFS5047341.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            complement(7749..8186)
                     /gene="zapA"
                     /locus_tag="ACG808_001716"
     CDS             complement(7749..8186)
                     /gene="zapA"
                     /locus_tag="ACG808_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFS5047342.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            8388..9305
                     /gene="rnhC"
                     /locus_tag="ACG808_001717"
     CDS             8388..9305
                     /gene="rnhC"
                     /locus_tag="ACG808_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFS5047343.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            complement(9561..10472)
                     /locus_tag="ACG808_001718"
     CDS             complement(9561..10472)
                     /locus_tag="ACG808_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFS5047344.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(10875..11453)
                     /locus_tag="ACG808_001719"
     CDS             complement(10875..11453)
                     /locus_tag="ACG808_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5047345.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(11473..12141)
                     /locus_tag="ACG808_001720"
     CDS             complement(11473..12141)
                     /locus_tag="ACG808_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5047346.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(12156..13226)
                     /locus_tag="ACG808_001721"
     CDS             complement(12156..13226)
                     /locus_tag="ACG808_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5047347.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(13397..14761)
                     /locus_tag="ACG808_001722"
     CDS             complement(13397..14761)
                     /locus_tag="ACG808_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFS5047348.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLTRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
BASE COUNT         4175 a         3142 c         2453 g         5001 t
ORIGIN      
        1 ctatatttta tgcttttttt cgttcatttc tcataattga aatcgattgt ttcaatgaac
       61 tccataatcc gccttcgcta tacacgagta cattcgattt gtacatgttt gcagacaaga
      121 tcatcaataa gaatgttacg gctacaagta ccaacaagga aatcaatgct ccacccatac
      181 cgactgtctc atttgctaag cgaaccggca taatatatga agataagaat ggaatatacg
      241 aagtgatccg aataacgata ttgtttggat ctgaagctcc tagtatcaat ccaagcatat
      301 atccgcctag agatagataa gtcactggca aaattgcttt tgctgtatct tctgctttgt
      361 tgaccaaaga gccacataaa gctgctaaaa cagaaaaaat caaaatgcca aagatcatga
      421 atagaagaga ataccacaag aatggtccaa tcaagttatc caatgagata ccgtctaata
      481 tatttttgac catttccata tctttgaatt gactgtatcc tataccaaaa ataataccgt
      541 ataatgccat ttgcgttaat gcaacaagta aaacacctgt tagttttccg taaaaatgct
      601 tttgtgcagt cgtactggac aggatcactt ccatgatccg cgtgcctttt tcagaagcga
      661 tctcttgtgc aatgatctgc gcatacgtca aaataataat gaacaagaca atcgttgcta
      721 cataacttac gacatattgg actgtcgagt tatcttctcc aactgtcatt tttccttttg
      781 aatcaaaatt gactttttgt cgagtcaaac ttgcttgttg gctcaagcta gctacttctt
      841 ctggtgaaat gcctaattga cttgctcgaa gcatcgattg caagccagtc agttgttgtt
      901 gtatcagtag ttgtgtactt tgtcctaatg aattttcgct ataaagcatc ccatttatct
      961 tatcattctc agttgagact actaaatacg catcgatttt ttcttccgaa agctcttttt
     1021 gtgctttttc ttcagaatca actgctttga actcaaaatc atctgttttg acttggctca
     1081 tttgctgact gatttgctca ttatcagcca atacgccgat tttattgaca tcactatttt
     1141 gttgagcaaa gtttcctgct acataaatga tacccagcat gataaaaggg actaatatca
     1201 tgatcaagaa agaaatcgac ttaacatttt ttaaatagac atctttggta ataatccaaa
     1261 acttacgcat cgatttcacc tgctttcaat ttgaaaatct cttccaaagt tggcggttgc
     1321 tggttaaaca taggaatgta gccgaactga gtcgcacgag taaagatttc tttcccagct
     1381 tcaggatctt ctaaggtaat ctctaacgta ttgtcttcat gaaggatcac ttttttcacg
     1441 ccttcgattt cttctacttc ctgttggctt aaaccagatt ccaagaataa cttcgtacgg
     1501 ccaaatgatt cacgaatctc atgtactttt ccattcaaaa ccatttttcc gtttcgcaac
     1561 atgatcaaat gatcgcagat tttttctaca ttatccatat tgtggctaga aaaaatgaca
     1621 catgaaccat gttttttcaa ttccaatatt ccatctttta gcaattcagc atttaccgga
     1681 tctaacccgc tgaatggctc atccaaaatg atcaatttag gttcatggat taacgtggcg
     1741 atcagctgga ctttttgctg atttccttta gacaaagatt ttactttatc cgtctttttc
     1801 ccttttacct gaaatttttc catccaaaaa tcgatttttg gttcaatctc ttttcttgat
     1861 ttccctctaa gttctgcaaa ataaagcagt tggtcttgaa tcgttacttt cggataaagt
     1921 ccccgttcct ccggaagata accgatgaca tcataatctt tctccccaag atgatggcca
     1981 ttccataaca cttctccttg gtcttgtgtc aaaaaatcca agatcaaacg aaaagtcgtt
     2041 gtcttcccag ccccgttttg gccaattagc cccatgattt ttccatcggg gatttggaaa
     2101 gagacatgat cgacagctgt gtagttccca aaagttttaa caagatcctt gacttctaac
     2161 atttcttcac tccttcttca agaaatcaca tatatcctta gacaccagac gtaagcgatt
     2221 tattatttac caacaaaacg taaacgctct ttttgttgta cataaaatga taggacactt
     2281 tactttcttt gtaaagtgcc ctttcctcat tcctgatgta atttatcaaa tacgttttgt
     2341 gcgacatttt tcggcagtcc gatttcttgt aattcttctg tcgtggctgc tgtgatattt
     2401 ttcaacgatt tgaactcttt aagcagcatt ttttttcgtt ttggtcctaa cccttcaatt
     2461 tcatctaatc gggaagcaaa actgttttta ctgcgtagct gccgatggaa agtgattgcg
     2521 aatcgatgga cttcatcttg gattcgctgg agtaagaaaa actctggtga atttcgctca
     2581 agcggtacta cagccaaatc tataccgaat agcaactcat ttgttttatg tttatcgttt
     2641 tttgccatgc cagcaatagg aatatcgatt cctaattgat tttctaagac atcttttgcc
     2701 acatctactt gtccttttcc tccatcgatc aagatcaaat caggtaaagg caacccttcg
     2761 cgaatcactc gtgaatatct gcgatagatg acttctctca ttgatgcata atcatccggt
     2821 ccgttgactg tttttatttt atatttccga tattcttttc ttgccggttt tccatcaatg
     2881 aatacgacca tcgcagccac aggattcgtc cccatgatat tagagttatc aaaagactcg
     2941 atacgtattg gggtcgggat attcatcgcg tttcctaaac gttcaactgc tccgatcgtc
     3001 cgttcctgtt ttctggcaat cagatcaaac cgttcattca aagcaactga ggcattctta
     3061 ttcgccaatt caactaattt tttcttttct cctcgttttg gttgcagcac tttcgtagct
     3121 aacaaagctt ctacactggg cttgtcgatc gtatcaggaa tcaagacttc tttaggaatg
     3181 aaatgctcat tttcctgata aaactgaccg aggtaagtca agaaatcttc ttctgcttcg
     3241 ttatagaaag gaaacattga aacatctcgt tcaattagct ttccttgtct tacgaaaaac
     3301 acttggacac acatccatcc tttgtcaatc gcataaccaa atacatctcg atccagtaaa
     3361 tcagtatttg tcattttttg gcgagtcatg attgtctcaa tggcacggat ctggtctcgg
     3421 tattctgcag ctttttcaaa ctccatgttt tcagcagctt tttccatttt ctgatggatc
     3481 tctgactcga ttttttcgtg tcctccgttt aaaaactttt tcacttcatc aacgattttc
     3541 gtatagactt tcggatctac gtcgaaataa taaggacaca gacattgtcc caaatgatag
     3601 taaagacaag gctccttcgt ctgactaggt ttgcattttc tcagaggaaa taatcgatcg
     3661 aggatcttct ttgtctcatt cgccgcaccg acatcgggat atggtccaaa atacagtgct
     3721 ttgtctttta gtactttacg agtaatcacc aaacggggat atttttcatt cgttatctta
     3781 aggaaaggat aggttttatc atcctttaac ataatgttgt atttaggatc atttttcttt
     3841 atcagattga tctctaagag caatgcttcg atattcgatt ctgtaacgat gtattcaaag
     3901 tcttcgattt cactaacaag ccgttccgtt ttcgtattat ggctgccagt aaaatatgag
     3961 cggacacgat tttttaatat tttggctttg ccgacataaa tgatcgttcc atttttatct
     4021 ttcatcaagt aacagccggg ctgatcagga agtaaagcta atttgttttt gattcgttcg
     4081 ttcatctggt actccccttc atattcaatt aaagttatta tagcatgtct gtcaaaaaaa
     4141 caatccagcc tgacaacgta attatactca aaaaatatta atttgaataa ccttaactca
     4201 accgcaaaaa aaccgaggac ggaactggat tcagtcccac cctcagctta tttttgctgt
     4261 aagtttttat aaatattgat cgatcatttg acgtagttgt tcttttgaat gaacaccaac
     4321 agctttttct actacttgac catctttttt caacattaat gttggaatgc tcatgatacc
     4381 gaattgttga ggagtttctg gattttcatc cacatccatt ttagcaattc tgaattcttc
     4441 ttcatcatat tcttgttcca actgatccaa aatcggtgct tgcatacgac atggaccaca
     4501 ccaagttgcc caaaaatcaa tcaatacaag tcctttatct gtttcttcat taaatgtctt
     4561 atctgtaatt acttgtgaca tatcaaagac ctccattttt aaaatctatg attagtatag
     4621 catttctata cttggatagc gaaccatctg cttaccttta gtaagcttta cttaaattta
     4681 acaattgtcg caccatttcc gccttggttt gccggtgcaa actcaaagct tttgacgctc
     4741 cgatggtttt taagataatc agtaattccc ttgcgaagtg cgcctgttcc ttttccgtgg
     4801 acaatagtca cttgaggata accagctaga atggcagaat ccaaatattg atctacctca
     4861 cttagcgctt cttcgtatcg ttttcctcga aggtccaatt gattcggcac gtgactacta
     4921 gattcagatc gtacagcatg gataactcgt tgtgtcggct ctttttgagg agcaacaggc
     4981 gtcagctcgt cttcggacac attcattttt aatataccga tttctacttg ccactgattc
     5041 ccgtttttac gtaaaagcgt ccctctttgg ccgtaagtcg taaccaacac ttcgtctccc
     5101 ggtttcaacg ttttttgttc tttcgccttc ttcaatactt tatttttctt caaagtttct
     5161 tcttgatgta gatttgctaa ttgggtcttt gcatcgatca actgatgctc tttcacattt
     5221 ccttgcccaa tctgctgttg cattttacga atatcagcaa tgattttttc tgctttttct
     5281 tccgcctctg acacgacttc gtttgctttt ttctttgctt tttccatttc tttttcacgt
     5341 tcttcaaaga aatagctgta agcttctttc aaatcatcgt gaagttcttg tgcctcagaa
     5401 acaaaatgac gcatttccag atattcggtc tctgccattt tccggcgatt ctctaaatct
     5461 gtgatcattt cgtttaaatc ttggctctca tcattcatca actgcttcgc ttcatcaata
     5521 acttctgtat caagacctaa tcggctagag atttcaaacg cattgcttcg tccaggaacg
     5581 ccaatcagca aacgatacgt tggacttagt gtatccacat cgaactccat gctggcatta
     5641 atcgtatttg cccgattgta tccataaact ttcaattcag gataatgcgt tgttgccatc
     5701 acataagcag actttttacc aagatcatct aagattgcga ttgctagagc agcaccttct
     5761 tgcggatctg ttcctgcacc gagctcgtca aataatacca gacttttttc attgactttt
     5821 gataaaatat ctacagtgtt cgtcatatga gaagaaaagg tcgatagact ttgctcaatc
     5881 gactgttcgt ctcctatatc cgcaaatact tcctcaaaaa taccgatttg actttcttca
     5941 cctgcaggaa tcggtaatcc tgcttgcccc atcagttgta acagacctaa agtcttcaac
     6001 gtgatcgttt tcccacctgt gttaggtcct gtaatgacga tggcttgata gtcttttccg
     6061 attgtgatgt cattagaaac tactttttcc tgatcgatca gtggatgtct tgcttgtttc
     6121 aagacgacat gattgtcttc actgattccc ggcacgatgg cttttacttc tttaccaaat
     6181 cgtgcttttg cattcatcag atccatttta ccaatcacat aagcattgtg gatgatttct
     6241 tgacgatggg gaacaagttc agcagaaagt tcacttaaaa tacgttgaat ctcatttcgt
     6301 tcagcgattt ggtattgacg caaacgatta ttcaaatcca ccacttgttt tggttcaata
     6361 aataaagtct gtcctgaagc actttgatcg tggactacac cgccaaatac accgcggtat
     6421 tcttgtttga ctgggataac gtagcggtca tttcgcatgg tgatgatcgc atcacttaaa
     6481 tactttgcat ttttcccacg gacgatccca tctaactgtt cacgaacagc ttgttcactt
     6541 cgccgaatgt tctgtcttat gctctttaac tcaggagaag cgtcatcagt cacgcgtccg
     6601 tcttcatcga ttgcttcctt taaacggcgg ctcaaaacag ggatcgtgac taactgatct
     6661 gcccacatat acaaacgtgc aaattcgatt tcactgtcag aaagatcatc aatgaaacga
     6721 ttcaattcgc ttgtcgtaga caatactctg gaaacttgtg caagctccac tccattcaag
     6781 tctgctccga tctcgatccg tttcatatgg ggacggatat tttcaagttt aggtataggg
     6841 atcccgcctc gcagacgttg cactttcaat ccatcctcag tttcttgaag ccagtttgtg
     6901 atcgtttgtt tctcactcac tggtaccaat tctgccagtt cttcttttcc ttgtgcagtc
     6961 actacaaatt gtctgaccat ctgtttgact ttttcaaatt ccaatgtttc aaggattcgg
     7021 ttattcattt tatcacctta tctttcacta tgaaaaaaga gctgtgccgg acatacgcta
     7081 cgcagccttc ttaaataaac tactctgtac aaaaaacgaa atattttcca gcactatttt
     7141 ccttaattca aaatattcgt tacccaaagc tcatacatct tgttggtaag aaatggcgtt
     7201 ctctcaacga taaaacgagc gaaactatgc tcgccgaatt gattttggac cacatctaat
     7261 ggaacaaagg caagtaactg caaaatcaaa aatatagtaa cgtaagccat gattagtgac
     7321 aggatgcctc ctgcaatcca gtctacctgt ctcaagatcg gcacatacgt caatccatgg
     7381 acaaaaaccc ccacaaatcg ggtaagcagc cagccaatta gcaaaataat cagaaaagca
     7441 gttcctgcat aaaaagcttc atctaggtga aaagcaaata cctgatcaaa aaatgctaac
     7501 tttgatgttg gggtgacagc tgggtatgga atgtataatt ccaaatgact tgctaatggt
     7561 ttgtaaaaat gctgggcagc aataaatgac aatacatatc caatcgcgaa tatgatttgc
     7621 aaagcaaacc cgcgacgtgc tccagaaaaa aacgcaatta gcaataaaaa taaaatcagc
     7681 aaactcagca tgctacgttc ctttctcttc taactttttt aaatttttct tattttgata
     7741 aaaggatctt aattagaaga tttttgttta atttgtcctt ttcgtttctc gtttaaaatc
     7801 tgttgtgctt ccacatgatt tttaatggta tgatctgctt gaccattttc tttcaatact
     7861 tcccgagctt cgttctcgat tgcttcaatg cgtttcacac gattttcaag ctctgtaaat
     7921 ttgatcattt ttttctttaa ttctgctgtt tcttcttcca gctccaagat tctttcttgt
     7981 ttcttcaatt gatccgaaag cgcattgaca gccatcaaga ttgctgcttg ttcattatcc
     8041 atttgtggtg ataattgttt caattctgct aactgttcat tgatcagctt cgttacgata
     8101 tccatatgat gcttggtttc ccgtccgata atagtatatg tttggttggc aatcactgct
     8161 ttgtagcgtg ttttttcgtg cgccatacga atcatttcct cctatttgat gaaagtttta
     8221 tttagtgtac catgaagttg gttctttcac cgttctccac aaatattttt cacatactta
     8281 actattatat gataaacttt aaaaaaatgc tatgtctcgc aatctcttag aaaacgaaaa
     8341 gtcgtgagtt gcggtatagt tttatgtata aaaacgagga gaattttatg agcaacgtca
     8401 ttatcaaagc aacaaacgac caacttagtc aaatgaaagc atactatcgc tcttatttgt
     8461 taaataaaca gattccttat actagctttg ctgcaaaaaa agacgggacg accattaccg
     8521 cttatacatc cggaaaagtg atgtttcaag gtacgaatgc agcaatagaa gctgataaat
     8581 ggggggaggc acaggataaa caagccaagc cagcaaaaac agcctcttct ttgccaaaaa
     8641 atatcagtca attatccgtt ttaggtagtg atgaggtcgg aaatggcagc tattttggtc
     8701 cgctaacagt atgtgctgct tatgtgaaac gtgatcagct tgcagaatta cgtaaacttg
     8761 gtgttagaga ttcaaaagag ttaaaagata gccaaatcat ccagcttgct gacatcttaa
     8821 aaaaaaccat cccctataaa ctgcttgtct tatctccaga aaaatacaat gagatccaac
     8881 caaaatacaa tgccgttcgg atgaaagtcg ctttgcacaa tcaagcgatc catttgctgc
     8941 taaaaaaaat cgagccagaa aaaccagaag cgattttgat cgatcagttt acgccggaag
     9001 caaattttaa aaaatatgcg cgtttagaaa aaaatcaatt acaagaaaaa ctgtacttcg
     9061 ttactaaagg agaacaatat catttagctg ttgctgctgc ttctatcatc agtcgtgcca
     9121 gctttttgga agagttacac aaagcatcag ctgaagctgg aatcaccctt ccatcaggag
     9181 ctggaacgaa atctgaccaa gtagcggcaa aactattgga gaaaggcggc atgcctatgc
     9241 ttgaaaaata tgccaaactt cattttgcca atacagaaaa agctttgaag ctgctaaaaa
     9301 aataatggaa ctgtgacaaa agtcatgtca cagtcccttg ttccgaataa acggtgttct
     9361 catgccaact cctcggtcac aagtcacaat agtcatcaaa catgagaagc tgttttttga
     9421 aattgctcct tgtgacgcgg agttaaacgg caggctcaca acctcttcct taaacggtgt
     9481 tcaaaagctt gcatctgcga taataagccc ggccaaacga aaaatatgag gagctatttt
     9541 tgtttggctg ggcttattta ttactgaaaa taagtggtca atgcttgtgt caaacttgca
     9601 gcaatctgct gctgataact gtctgttact aattccttta aatcactatc attattcata
     9661 taacccatct cgattaaaat agaaggttga agattttctc gaagaacttg atagttccca
     9721 gtggagattc ctcttgaatc cagaggcaga tcagtgaagg agtcagcaat tgtctgtgct
     9781 aatgtttcct cttgtccata gtaataatat gtcgtgattc cagttgcttc attcgcatat
     9841 tccgtagagt ctgcgtgtaa actgataaag acatctgcct ttttttcttc actgtagtcg
     9901 catatatctc caagctgtac taattcatct gtagtatggg tcaaataaac cgttgctcca
     9961 gcatcctcta gcgccttttt tacttttatg gctgtactta atgttatatc tttttcttct
    10021 gtttgttcat cattgcttaa tgccccagta tcatttccgc catgcccagg atctaataca
    10081 atcactgcat cagatagctc ttccggtttt gtttgtatat tctttgtgac tttggttcca
    10141 tcttcgaggg ctaaatatcc agtatagcca tcatttgtct tgatctgata agcatttttt
    10201 gtttcagaaa gaaactccgc cagctctccg ccgtttatag ttgcaagtgg ggtagttagc
    10261 gagtcatctg tatatagtgt acggtcattt tttccgattt tgattaatgt ctcttgttct
    10321 gtatcatcaa atgattcctt tgtctttgta accgtactgt tcgtactgct gaccgtttgt
    10381 ccaactatct ttttttgacc aaaaacatct atttgaaaac agataataat gataatgatt
    10441 gcaaatacaa aagctccaat tatttttttc attcttgact ctcacctcgt agatccagac
    10501 taagggtcaa aacttaaaat cagcttaagt acgcaaaaaa ggggctgtaa aagatataac
    10561 cgtagaaaat aacattactg ctaaaatgat ttttttcatt tttggcatat cctgaaatct
    10621 ccttttgact attcgtggtg attcatctac ccaataaata gtttccttca ttctacaaac
    10681 aattaatttc ttcgttatta aattaaaaca cttcctggaa caccgacata ataaataaaa
    10741 atataccctg ataatcatga gagaataaca aatttgtatt tatgaaaaac tacctatata
    10801 aaaaaaacaa gatataacga tcttcgtcat atcttgcttc ttcacctaac tatttttctc
    10861 cctatataac agctttacgc ttctcctaat acaccattca aatataaatc gatcaattgc
    10921 gccacattta gcggttcttc cacaggtctt ttcgaccgag tgatgtaagg tgctaacact
    10981 aagaaaaaat gccttgctgc gatttctttt gaaatcccat ttcttagcac agattggttt
    11041 gctgaaaaga tttcttcaaa aggttcacga taatttttct tccatagaaa gaaaagatga
    11101 tctcttgttt cctttgaaag attgctttca agatcgtgaa gcatcagaaa caaatcgacc
    11161 agatgtgtct caatcaaaaa ctgcgccatt tttgtcaagg ttacttgaaa tcccgtttcg
    11221 cttgtttgaa cgatttctct caaagctttt ccaacatctt ttaagtggtt ttctaaaact
    11281 gccgtatata gtttttcttt atcaccgaaa taatgataga gattgggttg ggtgatccca
    11341 actttttgtg cgatctctct tgttgaagtg ccgagatatc ccttggacat aaacaagtca
    11401 gtcgcttctt tcaaaatttt ttgctgtgta tctttgttgt atcgatggac catttttcca
    11461 cctgcttttt ggttattctt tttctttcaa tacaccatct tccatcataa atattctatc
    11521 gcatttgtca atcagccgtg tatcgtgagt aaccatgatc gttgctttat ttttgtcttt
    11581 tgattctttt gccaagatat cgactacttc tcgtgccttt tctgaatcca aactagcagt
    11641 tggttcatct gctagaataa tggacggatc attgtacaat gcacgagcga ttgcgaccct
    11701 ttgccgttct ccaccagata gttcttctgg atacttgttg atcaacttat ccacgccaag
    11761 ctgctcaaat aattcttttg cttctttttg acgatgttca tgtttgatct tatctactaa
    11821 taataactgc ttttttactg tcagaaaagg aaccagattg gaagcttgca aaataaagcc
    11881 aatttcttta aaacgtagtt taacgcgttc actttctttt tgattactga attcttgatc
    11941 attgataaat acagagccac tgctaggcgt ctgcaatccg cctgccaaag tcaaaaaagt
    12001 acttttacct gaaccagaag gaccgatgat tgcaacaaat tcccctttat cgatactcat
    12061 ggttgtttct ttcaatgctt taactacttg gtcgccatct tggaaacttt tttctacttc
    12121 tttaaattct attgctttca tgctcttcct ccttcttatc caattgcctt caacggatcg
    12181 atttttacga ttgtccgaac agaaaatagt gcgcctaaca cagctactac cagcatcaac
    12241 aagctaatga cgccaaaaaa cagccaattg ctttggaatg gaaccgcatc aggtagaaca
    12301 agggatgttc ccactgttcc taatagacca agaagaatac caacaaatga taaaatgaat
    12361 gtttgtgcca ctacagaacg cgcaataaat cctcctgtga tcccttgtgc tttcattaca
    12421 ccaaagatat taatcttttg catcgtcagt acataaatga aaatcccgat cacaatagcc
    12481 gcaataacga tcaaaaatcc gatcataaat ccaaaagtca atacttgcgc attatatcca
    12541 ggtaattcat tgatgaattt agaaattttg atctgttcta aatcttcagg taaattactg
    12601 attttacctc tgacaactat tgcgttaatc cgtgcatttt cacttgtatc ttctttttcg
    12661 aagcggatct cttggtaagc attgatggtt gtatagagaa ctggtgagac attgaatttg
    12721 gcatgatccg taaagccagt tagtttaaac gtcttgtcac taccagataa cttaactgta
    12781 tctcctacgg ctagaccata ttcttctttc aaactaatat cgcctactgc ctcatcatcg
    12841 ttatcaaaag cttttccttc aacaagattg ggcatgatga attgattttt atcgataccg
    12901 aagaagctaa cattgatttt tccttcttct gtactgtctt tactagtcac gactccaggt
    12961 gtctgggcaa gatacgccac tttcccacct tcgacttctt ctgctatctt cttagtaatc
    13021 atggacatcc ctagatttga atttgcatct ttcgaaagca caatcgaatc tgcctcccac
    13081 ttgtcgactg ctgttcgatt atcttgagct aagccataag ctaacccagt caaaaaaaat
    13141 accaggtagg cgatcaaaaa catcacccct gcaactaacg cataacgtaa ctttgaatgc
    13201 ataatttcat ttaatgctaa aaacatttaa ctcctcctcc tgaaataatc attttataca
    13261 acaattttat cacttgataa atttttggcg aatattatgc tcaaaaaatt cttccgctat
    13321 ttcattatat gaatttttta ttctcgattg attaatattt attatatccc cacacaataa
    13381 aagaccgtga tatttctcac agtcttttat ctactctatt gctatttgct ctcttagcta
    13441 gtactttaaa agctaactca ttgaggtagt gaaaacgaga ggtgtgttca gacagaaatc
    13501 caaaatcctc aactgctagc tgtggaatcg tagttttgtg tttcacgata tccgcaagca
    13561 ggttcaaatc tgataataag aattcttggc ttatcagctg tccgccaatt atttctaatg
    13621 tatccggatg gtagatcagc tttgcattta ctgttttgtt ttcttcatcg tattgtggaa
    13681 atagatcaaa ttctcctgaa cacgaaccgg tattttgttc caagaatggc gcttcatctt
    13741 ctgtcagccc tacagttcct aaaaagtagt ctataatgac tgctgctgtt atcttttgtc
    13801 tgaggtcgta agcggttggc tgaccactca aagttagcgc tactgctctt gccatatgta
    13861 cagctcgagt aactaaaggt aaaaagagat gccgtttatt tttgtcaaaa ctgactggga
    13921 cgatatctcc aatcgcatag atgtccggat cacttgtctg cagatattca ttgactatca
    13981 cagtctcatc ctcatacaaa gccacttttt cttttaacaa tgtcgtatta ggacgagttt
    14041 gggcagaaaa aatgaccata tctgccttta ttgcttgaga tagaagttgt acggatacta
    14101 ctttctcttc tgattttttc caatcgatca agaattcatt tggataaaaa ttgatttgct
    14161 tagtttgcag ctgattttga aaactgttga tcatatcctt atcgaaatat cttgttaaaa
    14221 cattcggcat ataatcaact agatgaataa tgtatccttt atttctcaaa gcatctgcta
    14281 attccaatcc aatataacca gcaccgaaga tcactacttc ttttgcttct tttaaagcgg
    14341 caagtacttg cttagattgc actagatttt tataggtgta gacattttca gcttcaattt
    14401 gtttatacaa ggtgacagga gaggaacctg ttgctacgat taatttatca taggttatgg
    14461 tctcgttttt tccttctttg gatgcgagga tgagctgatg tttgtctgga tcaagttcag
    14521 ttactctcca ttcggtgatc agttttgccc cactttcttg aagagtccgc atgttatggg
    14581 caacttcagc taaactacgt atctttcctt ttaaataaag gttgattcca tttgaaacat
    14641 agcctagtcc atctttatga tcttcatcga tcagtaaaac ttctgctttc ggttgaagtt
    14701 ttcttagatt tagggcagct gtgatgcctg catgggaagc accaatgatc acgattttca
    14761 tgttttattc a
//
LOCUS       DAZUMK010000060        14725 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000060 DAZUMK010000000
VERSION     DAZUMK010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.061"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..604
                     /locus_tag="ACG808_001723"
     CDS             <1..604
                     /locus_tag="ACG808_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFS5047349.1"
                     /translation="VRRRKLIANDILIILKNEFGIEVKDYFIDLDLLQLINSVKRMYS
                     GDDNGVDLYKFVTKHIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFK
                     KMFKSRDGITLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYV
                     ICSRAKNNLFLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            804..1118
                     /locus_tag="ACG808_001724"
     CDS             804..1118
                     /locus_tag="ACG808_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS5047350.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            1139..1516
                     /locus_tag="ACG808_001725"
     CDS             1139..1516
                     /locus_tag="ACG808_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS5047351.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            1526..2299
                     /locus_tag="ACG808_001726"
     CDS             1526..2299
                     /locus_tag="ACG808_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047352.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            2321..3724
                     /locus_tag="ACG808_001727"
     CDS             2321..3724
                     /locus_tag="ACG808_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFS5047353.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3906..5090
                     /gene="mobT"
                     /locus_tag="ACG808_001728"
     CDS             3906..5090
                     /gene="mobT"
                     /locus_tag="ACG808_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFS5047354.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            5087..5377
                     /locus_tag="ACG808_001729"
     CDS             5087..5377
                     /locus_tag="ACG808_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047355.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            5374..5595
                     /locus_tag="ACG808_001730"
     CDS             5374..5595
                     /locus_tag="ACG808_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047356.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5637..6416
                     /locus_tag="ACG808_001731"
     CDS             5637..6416
                     /locus_tag="ACG808_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HFS5047357.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            6477..6977
                     /locus_tag="ACG808_001732"
     CDS             6477..6977
                     /locus_tag="ACG808_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFS5047358.1"
                     /translation="MEMKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEE
                     YAIHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENK
                     EDIHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHG
                     VFEYCQ"
     gene            7033..7671
                     /locus_tag="ACG808_001733"
     CDS             7033..7671
                     /locus_tag="ACG808_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6037 family protein"
                     /protein_id="HFS5047359.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            7743..8138
                     /locus_tag="ACG808_001734"
     CDS             7743..8138
                     /locus_tag="ACG808_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS5047360.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            8122..10575
                     /locus_tag="ACG808_001735"
     CDS             8122..10575
                     /locus_tag="ACG808_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5047361.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            10572..12599
                     /locus_tag="ACG808_001736"
     CDS             10572..12599
                     /locus_tag="ACG808_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFS5047362.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            12596..13618
                     /locus_tag="ACG808_001737"
     CDS             12596..13618
                     /locus_tag="ACG808_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFS5047363.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            13635..14564
                     /locus_tag="ACG808_001738"
     CDS             13635..14564
                     /locus_tag="ACG808_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS5047364.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
BASE COUNT         5013 a         2457 c         2911 g         4344 t
ORIGIN      
        1 ggttcgaaga agaaagctaa ttgcaaacga tattttaatt atattaaaaa atgaatttgg
       61 aatagaagtt aaagattatt ttattgactt agatttatta cagctaataa acagtgtgaa
      121 aagaatgtat agtggagacg acaacggtgt tgatttatac aaatttgtta ccaaacatat
      181 atttgttaat cttaatattg acgagaaaaa ttacccgtct atttttaagc actatctatt
      241 ttttattaac gaagtagaag atcggataaa aaataattca ttatctagta gtgtatctag
      301 ttttaagaag atgtttaaat ctcgagatgg tatcacactt actacagcac ataaagttaa
      361 aggaaatgag tatgatactg ttatcgctat taatcttcta aacgggaaaa ttcctcattg
      421 gaatgaaatt tttaatgttt ctgataaagg aattagttca gcgagaaaac tactatacgt
      481 aatttgttca agagcaaaaa ataatttatt tcttttctca gaggtgggtt attttacgag
      541 taaaggagac ttgctttatc ccactcaatt attagataaa atagaatttg attataatat
      601 ttaaaatacc taggtagtcg ttgttttaaa tatagtagga atcggacgct cagtacggag
      661 cgtctatttt tttacccaat tttgaaagga agtgaactta tgaaaataaa aaatcaagaa
      721 tcaaaaggtc gctctccccc ctttaaaacc atcacacaaa taagattcat tcaaacataa
      781 aaacagaaag gataggtaaa aatatggaac ttaaatatgt cattcccaat atggaaaaaa
      841 catttggaca attagaatat gctggtgaag ggaacattga acaacgtcga ataaatggca
      901 gaccaaccat tctctctcgt agttacaatt tatactcaac gattcaacga gcagatgata
      961 ttgttgtcat tcttccctcc gaagctggcg agaaacattt tgaatccgac gaaaaagtca
     1021 aactcatcaa cccacatatt accgcagaag gttataaaat tggcaatcga ggatttacca
     1081 attacattat gcacgcagac gatatggtga aagcataaga aaggaaaggt gaaaaaatat
     1141 gagattagca gaaggtattg tgattgaaaa agaccagaca tttggcacat tgaaattttc
     1201 cgccttgcgt cgtgaggttc gtatccagaa tgaagatggt acggtttccg aagaaattaa
     1261 agaacgtacc tatgacttaa aatcaaaagg acaaggacgc atgatacaag tcagtatccc
     1321 cgcttctgtt cctttgaaag aatttgacta caacgcagaa gtggaattaa tcaatcctgt
     1381 ggcagatacc gtagcgacgg ctacttttcg aggggcagac gttgattggt acgtgaaagc
     1441 agacgatctt gtattaaaag gacagacaaa acctcatcag aaatataaaa aagaagcgtc
     1501 cgaaagtgaa aaataggtgt tgcttatgac aaacacacca ttgattttga aagaaattcc
     1561 gaaggatgaa gccatttctt ttattcggca ataccattat tcaaagatac tccctagatt
     1621 gtgtaaatac tttctaggga ttttttcaga agaaaaatta ttaggcgttg tggaattagg
     1681 ctggggtacg caacccctac aaaccatacg caaactcttt cccgacagtt cccttcaaac
     1741 cacagattat ttagaaattg gaaagatgtg ttttcttcca gaaatgaatc aaaccaatta
     1801 ttttggcagt caagctcttt cagctctaat taagtggttg aaagaacata cggactgtca
     1861 ttttttatat acactggcag atggaattga gggaaaatgt ggctatgtct atcaagcgtc
     1921 caacttcttt tactgcgggt atttcaagac aagtgtctat cgtgataagc agtcatggga
     1981 aaagatacac cctcgaagtg ctcgcctttt attagaggaa aatgctcgct ttgaacaggt
     2041 agagaaaaag cattggcttt cccaggcatt ttgcgaatac aaagggattg agaaaatcaa
     2101 tggacgcatg tttcgctatc tctatccgtt gacaaaagaa gcgaaaaaac tgttaggaca
     2161 cactctctat cgacgtcatt attatccgaa agaaaaaaat ttacgctttg aaaagcgaat
     2221 cgcttatcag aaatatgaag ccatttctca accaactttt gataaacagg ctcgtatcta
     2281 caatacacaa ttattttgat aggtggaaag gaggattttt atgccattgc ttcatgacag
     2341 aggagagcga atcaaagcca acgataagca cttggtctat cattttgtac taatttcatt
     2401 gctggtagtt tttctagtcg tcctactccc ttttcattgg agagcgttac aatcggtgaa
     2461 ctggcaagca gttcagttag atacattttt ctatcaactt catacgccat acttttggag
     2521 cagtattctc actgctcttt ttgtatgcgg acttatcagc tggctttatt atcactatcg
     2581 tatagattct ataaaacagt tagagcaccg tcaaaagtta gctcgtatga ttttagaaaa
     2641 tcattggtat gaagccgaag aaactcaatc agagggattc tttaaagatt tacccagcaa
     2701 gaaaaccaaa caacgaatcc gccattttcc taaaatatat tatcgaatga aagatggact
     2761 aatccatatt catgtagaaa tcaccttagg gaaatatcaa gatcagcttt tgcacctaga
     2821 aaacaaacta gaaacaggat tgtattgtga attagtggaa aaaacgttga aagattctta
     2881 tgtagagtat gtcctacttt atgatactat tggcaatcga attggcattg aagacgtggt
     2941 agctcaagat ggacgtgtca aattgatgga atcggtctat tgggagttcg attctctccc
     3001 tcacatgtta attgctgggg gcactggtag tggaaaatcc tattttattc tcacattgat
     3061 tgaatctctc ctgcatacaa atgcaaagct ttatattctt gaccctaaaa atgctgattt
     3121 ggcagactta ggggcggtca tgaataatgt ctattaccgt aaagaagata tgttggcatg
     3181 tatcaatcaa ttttacgaag atatgattga tcgcagtgaa accatgaaac aacaccctaa
     3241 ctataaaaca ggagaaaatt atgcttatct tggtttgtct gccaattttc ttatttttga
     3301 tgaatatgtg gcaatgatgg atatgctagg tcgtgaaagt accaccgtta ttcataaact
     3361 caaacaaatt gtaatgttag gacgacaagc aggcttcttt ttaattcttg cctgtcaacg
     3421 tccagacgca aaatatttta gtgatgggat tcgggacaac tttaacttcc gtgtggcttt
     3481 gggtcgtatg tctgaaatgg gctttggtat gatgttcgga agcgatacac aaaaacaatt
     3541 tttcttaaaa cccatcaagg gaagaggtta cgtggataca ggaaaaagtg tgattagtga
     3601 gttttataca ccccttgtac caaaaaggta cgatttctta ggagaaatag gaaaagtcat
     3661 tcaaaaaaag caatctgagc ctgtaccgca cgaagtgaaa ggtacaggtt cagacttgct
     3721 ttgattggtg tggcgttagc cacgccaatc ctttagcccc ctctatctaa caggggggca
     3781 caaatacaac tgaaaacagg caattctcat caaccaatgt tgatggtaca aaggattgcg
     3841 gaaaatatga aaggtgtaaa ctcaagggtc atagtttaca tcttttttag attggaggtt
     3901 tgcgaatgga aaacgaaaaa atttggatta aggaattgaa agaaaagcga ctggcttatg
     3961 gcgtttctca aaataaactg gcggttgctt ctcatattac cagaccttac ctatcggata
     4021 ttgaaacagg caaggcagtc cctactcaac aggtgaaaga agatttactg aacgctttgg
     4081 aacgcttcaa tcccgataac ccattggaga tgttattcga ctatgtacgg attcgattcc
     4141 ctaccaatga tgtggctcaa attatcggtg aggttttacg tttgaatatg gattatatgc
     4201 tacacgaaga ctttggctat tattcttatc ccgaacacta tcgtttcggt gatattgttg
     4261 tcctagtctc tcatgatgta agtaaaggtg ttttattaga actgaaagga aaaggctgtc
     4321 ggcaatttga aaactttcta ttggctcaac atcggagctg gtatgatttc tttcaagatt
     4381 gcatggaaca taaaggtatt ttcaaacgct tggatttagc tatcaacgat aaaacaggga
     4441 ttttgaatat tccagatttg gcaaagaaat gtaaacagga agaatgcatt tccgtctttc
     4501 gaagctttaa aaattatcgc agtggtgaat tggttcaccg tgatgaaaaa ccatacatgg
     4561 gaaacacact ttatattggc tcattaaaaa gtgaagtcta tttctgtata tatgagaaag
     4621 attatgagca atacgtgaaa aatgatgttc ccttagaaga cgcagaagta aagaatcggt
     4681 ttgaaattcg gttaaaaaat gaccgagcca cccaagccat gaaagattta ttggcacatc
     4741 aacaggcgga aaaaacagcc tttgaaatta tcaatcgcta tatccgcttt gcagataaag
     4801 acgacactaa acgtcggagt gattggaaaa ctaatgaacg ttgggaatgg tttatcggta
     4861 aaaatcgtgg agcattacgc ctaaccacac agccagagcc ttattctttt gaacgaacat
     4921 tgaactggtt acatcatcaa gtcgccccta cattaaaaat cgcaagcatt ttagatgttc
     4981 tcaacggtac aaccattatc tccaccatga ttcaggaagc caaactgaca gaaaaacatg
     5041 agaaattaat tgaacaacaa catctagcca tggaggattt gattacatga gccttttaac
     5101 aaaagaatta aagaagttag ggtttcagtg tggcattgag tttcaagcgt atatacaaaa
     5161 tacagggaaa tatacgtctc tcattattga gggaaaacgg caagctggtg ataccatcta
     5221 tacctatgat ttttacaagg tgagatttta taataattat accaatcgag taaccgtcta
     5281 tggagagcat ttaacccctt ttcaattatt aaggcgtgta aaaagctata tctattatcg
     5341 agaaaaatat cttaaggaaa ggaggacaat cacatgaatt ttgggcagaa tttgtacaac
     5401 tggttcttgg ataatgcaca gtcattggta ttgttagcga ttgttgtcat tggactgttt
     5461 ttaggtttta aacgagaatt ttcaaaactc attggcttct tgattattgc tctaatcgcc
     5521 gtcggacttg tgttcaatgc agctggcgtg aaagatgtgt tattaaactt atttaaccga
     5581 atcattggtg cttaacagat atttttcatg ataaaatagg aagtatatat tttgttatga
     5641 aaggatttat gaatatgttt acaaatgatc aatttaaatc tcttttaatt aaaacattag
     5701 aaagaaagag ctcttcaaat tattatgaag gaggtaacga gattgtttct aaaatgaaaa
     5761 tatcagaggt aactttagat gagactgacg attttactta ttataaagga tataaaaagg
     5821 gctggaatac gctttgcaca tatcttaaaa taagagttcc ttttgatgat ttagactttt
     5881 ttgaaagtca taaagatttg attacccaga cagcaagctc catatatgat aaacaaggtg
     5941 ataatgtatt agtggatact attttagtac cacttccaga gaattacgaa gtaattaact
     6001 ttagtcagtt gaaaattagt gatgtggttt cacaagctat tgaagacgct gagtctttta
     6061 tgagtaatgg tgaatatcaa cgtgcctttg acagagttca cactgctttt catggatatt
     6121 taattgaaat actaaaaaaa tatgaaatta cagttcctag agacgaaaat ctatctaaat
     6181 tatatagtag gatacaacaa ttaattgaaa aagaaattca acctactgaa cttgctgata
     6241 tagtgaagac aacgataagg tcatcaaatg gaatgattag ctctcttaat gaagcaagaa
     6301 ataggcattc acttgcacac cctaatacaa atattattgg aaagagagaa gcaaaactaa
     6361 taataggtat ttcaagtaca gttacagatt atattagcgg ataccttgat aagtagcgtc
     6421 attaactgat tgctactttt tttatgacta tttttataaa aaggatgtga taagaattgg
     6481 aaatgaaagt ctatgtggca aatttaggac ggtataatga aggtgaatta gtcggagcat
     6541 ggtttactcc accaattgat gaggaagaaa tggcagaaag aatcggttta aatgaggact
     6601 acgaagaata tgcaatacat gattttgagc ttccttttga tgtggacgaa tacacgccga
     6661 ttagtgaaat caaccgtcta tgtgaagcca ttcaagaaat cgaaggtaca cctatttata
     6721 atgaactcaa agaaattcaa ggcatgtggt ttagtagctt ggaagaatta ctggaaaaca
     6781 aagaggatat tcattgttat agtgattgtg attcgatgga agatgtcgcc cgttattacg
     6841 tggaagaaac aggacagctt ggtgaagtcc cttccaattt acaaaattat attgactacc
     6901 aatctttggg acgtgatatg gaaatagagg gtaattacct agttacctct catggggtat
     6961 ttgaatattg tcagtaagtt taacaatccg tgttataatt aaattaataa atagcacgga
     7021 gaggtataaa ttatggtaaa tgtatttgaa aatcttaaac tattaaaact agacatgaaa
     7081 gataaaggct gggtgataga ttccttttac tttcgttaca aacagcaaaa ttatattgta
     7141 cttgttaagc tatttgaaaa agaggaaaaa gttccagaat atgctctgtt aaaactagaa
     7201 tttttaaagg aaaacgattt ttcagatatg ctagctgtct atgctaattc ggtaaaatta
     7261 tatacagaca cgaaaacaat aagagaatat tttggtatcg aatacagtag taacttggga
     7321 gatgttttat ttcaatttag ccaaactctt gctcgattta ttccgacaga agttagtgag
     7381 aagaaaaatg aagaccaaaa agaagccatg tgtttctcat taagtcaaag tgattcagaa
     7441 gatcccagaa aaaaatattg tttttcagtt agaagaaatc ctttaaaggg aaatggtgag
     7501 ctgggaaaac gaagtccttt taatgacaat aaaacgagat tgtatagacc cagcttatat
     7561 gaacgattag ggtcagatac caatttaagt ttcagatatt caatgaatcc agatgatgaa
     7621 gaaactgatg aaggtattat tgcaaaatgg acaaaaaata aaattgaata gttaaagcat
     7681 tgaaagagca atcacctatt ttgattgttc tttttctatt tttacggaaa ggacgtgaag
     7741 acatgaaaaa aataagaagt tacaccagta tttggtcagt agaaaaagtg atttatgcga
     7801 ttaatgattt acaactccct ttcccaatta cctttacgca aatgacatgg tttatcgttt
     7861 ctttgtttgt cgtcatactc tttggcagtt taccaccact ttctctgatt gacggagcac
     7921 tactcaaata cttagggatt cccattgttc tcactgggtt catgtgccaa aagacctttg
     7981 acggaaagaa accctatgga tttctaaagt ctatcattac ctatgctcta cgccctaaag
     8041 taacctacgc tggcaaacaa atacgattaa aaaaagaaac aatgaatgaa tcgattacga
     8101 ttgtaaggag tgaacaacta gatggcatat ccgattaaat atatggaaga taatctcgta
     8161 tttaatcatg atggcgaagt attcgcttat tatgaattaa ttccctataa ttacagtttt
     8221 ttaagttccg atgaaaaaat tcaagttcat gataatttct gtcaattgat tgcccaaaat
     8281 cgtgatggca agattcatgc tctgcaacta gcaaccgaat caagtattcg agccacacag
     8341 gaacaatcga aaaaagaaat cactggacga ttaaaagaga tagcttacga aaaaatagat
     8401 gaacagaccg aagcgttaat ttctatgatt ggtgacaatc aagtagacta tcgctttttt
     8461 attggtttta aactctacct caatgaacag gaagtatccg taaaaaatgt tgggaaagaa
     8521 attcaaaaag catttgcgga attctttcat ggtgtgaatc acaaattaat gggcgatttt
     8581 gtttctatgc ctatggcgga aatcgaacgc ttttctaaga tggaacagtt gttagaaagt
     8641 aaattatcca gacgctttca gattcgtaaa ttggagaaag atgattttgg ttatattatt
     8701 gaacatcttt acgggcaaac aggtgtccct tttgatgact acgactacca tttaccgaaa
     8761 cgatacgaag ataaaaaagt gttggtaaag aaatacgata ttctcaaacc cactcgctgt
     8821 ttaatcgaag aaaatcaacg ctatttacgg attgaacagg aagataccac tacttatgtg
     8881 gcgtatttta caattaatag tattgttggt gaattggatt tccctaatag tgaaattttt
     8941 tattaccagc aacagcagtt tactttcccc gttgatacca gtatgaatgt tgaaattgtc
     9001 gccaataaaa aagccttatc taccgttcgc aataaaaaga aagaactgaa agatttagat
     9061 agccacgcat gggaaagcga caacgaaacc agtactaatg tggtggacgc tttagaatct
     9121 gtcgatgaat tagaaagtaa tttagaccaa accaaagaat ctatgtacaa actaagctat
     9181 gtggtacggg tatcagcaaa gaatttggac gagctgaaac aacgttgtga tgaagtaaaa
     9241 gatttttatg atgatctgtc tatcaaatta gtacgacctt ttggcgatat gctaggcttg
     9301 cataatgaat ttattccctc aagcaagcga tatatcaatg actatattca atatgtcacg
     9361 tcggattttt tggctggtct tggtttcggt gctacacaaa tgattggtga aaattctggt
     9421 atttacgtcg gttacaatct cgatacaggc agaaatattt atctccaacc tagccttgca
     9481 agtcaaggaa ttaaaggctc tgtaacaaat gcattagcgt ccgcttttat tggttcatta
     9541 ggtggcggaa aatcattctc taacaatatg ctggtctatt attcagtact ctttggcgga
     9601 caggcaacca ttgttgaccc aaaagctgaa cgtggtgaat ggaaagaaac cttacctgat
     9661 attgcccatg aaatcaatat tgttaatctc acgagtgaga acgacaataa agggttactt
     9721 gacccttatg tgattttaaa actcccgaaa gattcagaaa gccttgccat tgatattctc
     9781 acgttcttaa caggtatttc cagccgagat agcgagaagt ttcctgtcct gcgtaaagct
     9841 attcggagtg taactcaaag caaacaacga gggttgttat tggtgattga tgaattgcga
     9901 aaagaagata cgcccatcag caatagtatt gctgaccata tcgaaagctt tgtcgattat
     9961 gattttgccc atctgttatt cagtgatggc agtgtcaccc aatccattag cttggaaaaa
    10021 caactcaata ttattcaagt ggcagatttg gtcttacccg attccaatag ctcttttgaa
    10081 gaatacacta ccatggaatt attaagtgtg gcaatgctga ttgtcattag tacctttgct
    10141 ttagacttta tccactctga ccgttccatt tttaaaattg ttgaccttga tgaggcttgg
    10201 agttttttac aagtggcaca aggaaaagct ctatctatgc gattggttcg agcaggacgt
    10261 gccatgaatg caggggtcta tttcgttact caaaacgcgg acgatttact agatgaaaag
    10321 atgaagaata atattggctt gaaatttgcc tttcgttcca ctgacctagt ggaaatcaaa
    10381 aagaccttag aattttttgg tgtggacaag gaagatgaaa acaatcagaa acgcttacgt
    10441 gaattagaaa atggacaatg tttagtttca gacatctatg gtcgtgtagg ggttatgcag
    10501 tttcacccga tttttgaaga gttgcttcat gcctttgata ccagaccgcc tgtgcgaaat
    10561 gaggtggaag aatgacacaa aaacaaaaga aatttcttca ctatagttgg ataacgctcc
    10621 tttgtgtggg cgttatttta cttttattag gcacgctggg caatgcagta caagcgacag
    10681 gcttagttga tgaaaccatt gatacaagca atgaatactc caagtatggt ttgaatcaat
    10741 atcagcttga ttattatgtg gacaatagct ggggctggct tccatggaat tggagtgatg
    10801 gcataggaca atcggtgatg tatggattgt atgccattac taattttatt tggacaattt
    10861 ctctttatct atcgaacgcc acaggttatt tggtgaaaga agcctatgcc ttagatttca
    10921 ttagtcaaac atcccaagca attggcgaaa acatgcagac gattgcaggc attacaccga
    10981 atggcttttc cagttcaggg ttttatgtcg gtttcctcct tctttttatt ctcattgttg
    11041 gaatctatgt tgcctataca ggattggtta agcgagaaac gacgaaagtg atacgagctg
    11101 ttttaaactt tgtcgtggtg tttattctat cggcttcctt tattgcgtat gcacccgatt
    11161 atattgcgaa aatcaatgat ttttccaaag atgtcagtac cgccagtctg aatattggga
    11221 caaaaattgt tcttcctaac acggatagtc aagggaaaga cagtgtggac atgatacgtg
    11281 acagcttatt ctctgttcaa gtaaagcaac catggctatt gcttcaatat ggctccactg
    11341 atgtagaagc gttaggggaa gaacgtgtgg aaagtttatt gtctaccagt ccagatacaa
    11401 acaatggtga agatagagaa aatgtagtga ttgaagaaat tgaagatcat gacaatacct
    11461 atttaaccct accgaagacg atttccagat taggaacagt cttctttttg tttgtattca
    11521 atattggtat ctcgattttt gttttccttc taacaggaat catgattttt tcgcaagtgc
    11581 tatttattat tttcgctatg tttttgccaa tcagtttcct attaagtatg ttgccgagtt
    11641 ttgatggcat gggcaggcat gccattacca agttatttaa tgtgattatg acaagggctg
    11701 gtattacctt gattattacc gttgccttta gtatttccac tatgctttat tcgctgtcag
    11761 caagttctcc ctttttcatg attatgtttt tacaaattgt gacctttgca gggatttatt
    11821 ttaaactggg cgatttaatg agcctattca ctttacaaag caacgactct caaaacatgg
    11881 gcagacaagt gtttcgcaga cctcgtcaaa tgatgaatcg acaaaccaga agatttcaac
    11941 gcagtatgag taagattttt cgtggaaaag cagggtcaac gaagcaaaag acaatgacaa
    12001 caaagcagaa tcaaaccaat aaagctaacc atactcgtca aaatgaacgg actacaccaa
    12061 agaaagaaac agcactttcc aatgtgaaaa agcaacaagg cggtatcggg caacgattag
    12121 gtgaaaagac agcccatgta ttggacacga aaaatcgtct agtcaataaa agaaagcagg
    12181 caaaacaaca gatacaggcg acaccaaccc atgtcaaata tgcccttcat caaggaaaag
    12241 agaatgtcac tcataatgta acagacttta aacaatctat ggcagatacc aaagctcaac
    12301 gcaaacaaca gcgtatgaac cagcaggagc aaaaacgaaa gaacattgct caaaagcgtt
    12361 tggagctgga taaagcaaaa gaaaaaagac gaaaagaaaa cccatcatct cctgttcaga
    12421 ataaccagca gcatgttcgc aagcgtcagc aaaccgtcac gcctgttttg tcacaaaaca
    12481 ctaaacgtga aaatcatcag cgaccattga aaaaagagca aaaacaaaag acaccaccga
    12541 agcaattcaa tcagaggaag ctaaataaaa gaacggataa gcgaggacaa caaacatgaa
    12601 gctaaaaata acagtgattt gtagtgtggt cttctctctc ttcttttttc tcttattatt
    12661 cctagtcatt ttctttgcag atgatacgga cagtggcgaa aacaacaagg attcttctat
    12721 tccacaaggc ggtgtgaccg tttcaccaga agtgttggca caccgtccgt tgattgaaaa
    12781 atatggtaag gaatatggga ttgaagacta tgtttcttat atcttagcca ttatgcaagt
    12841 ggaatcgggc ggtacagcag aagatgttat gcaaagttcc gaatctttgg gtttaccgcc
    12901 caacagtttg agtaccgaag agtccatcaa acaaggggtt aaatatttca gtgaattatt
    12961 aaccagtgca gaacaacaag gagtggatat agattctgtt atccaatctt ataattatgg
    13021 cggtggcttt ttaaattatg tgaggagtca tgggaaaaaa tatacctatg aattagccga
    13081 acagttttct aaagagaagt cgggtggtca aaaagcagac tatcccaatc ctatagccat
    13141 acctgtgaat ggtggttggc gatataacta tggaaatcag ttttatgttc agttggtttc
    13201 acaataccta accgacactt ccccaacaga atttgatgat gaaaccgtgc aagtcattat
    13261 ggacgaagcc ttgaaatacg aagggtttcc ctatgtcttt ggaggggcgt cgcctaccac
    13321 ctcttttgat tgtagtggct taatacaatg ggtgtatgac aaagctggca tttctctccc
    13381 tcgtgtcgcc caagatcagt atgacgctac gcaagaaatc tctatggaag aagcccaagc
    13441 aggcgacctt atcttctttc attcaaccta taatgcaggc acatatgtga ctcatgtggc
    13501 gatatattta gagggaaatc gcttttatca tgcaggcgac ccaatcggtt atggcgactt
    13561 aagcagtcgt tattggcaag accatctgat tggggctaga cgtgttattc ataactaaga
    13621 aaggaatgaa tcaaatgatt ataaaaatcg agcgaaaaga aaagaaggag aaaccaaaga
    13681 aatctaaaaa accgagcaag caaagaaaag ttccgatgat taaagttggc acgcataaga
    13741 aattaacctt tgttttatgg gttttattga ttggtagtgt cggatttgga atctataaaa
    13801 attttactgc cattgacact catactgtcc gtgaaacaga aatcattaaa cagcagattg
    13861 tcgataccaa ccaagtagaa agttttgtga aatcctttgc gaaagactac ttttcgtggc
    13921 aacaatccca agaagccatt gataaacgaa atgaaaaatt gacccattat ttgacggaag
    13981 aattgcaggt attaaatgaa gaaatgattc gtaaggatat tcctactagt tctagtgtga
    14041 acgatataca ggtgtggcag gtttctcaag tgaatgagaa cacctttgag gtactgtttt
    14101 ctgttgaaca agttattacc gaagacaaag acaaggaaac tatatcgtct agctttcatg
    14161 tggttgtcca tatagatgaa tcagataata tggttattat caaaaatccg acaatgagta
    14221 agaaacctca aaaatcggac taccaaccca aacagctaga aagtgaccat acagtagata
    14281 cagaaacgat ggacgaaatc actagctttt tagaaacgtt cttccagctc tatcctaccg
    14341 ccacagaaaa agaattgact tactatgtaa gtaatcatgt gttacctatg attaacaagg
    14401 aatatgtttt cgaggaattg gtgaatccta tctttaccag aaaagataac caagtaatcg
    14461 tgaatgtcgc tgtgaaatat ttagatcaag aaacaaaggc aacccaaatc tcgcaatttg
    14521 agttgatatt agaaaaacaa gataattgga agattgtaaa ataacaaata ttggtacatg
    14581 attacagata ctttgtaatc atgtactctt tttgataaaa aattggagat tcctttacaa
    14641 atatgctctt acgtgctatt atttaagtat ctatttaaaa ggagttaata aatatgcggc
    14701 aaggtattct taaataaact gtcaa
//
LOCUS       DAZUMK010000061        14660 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000061 DAZUMK010000000
VERSION     DAZUMK010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.062"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            282..602
                     /locus_tag="ACG808_001739"
     CDS             282..602
                     /locus_tag="ACG808_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFS5047365.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGLLLISIIGIKI
                     TSGH"
     gene            763..1668
                     /locus_tag="ACG808_001740"
     CDS             763..1668
                     /locus_tag="ACG808_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5047366.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSEGTIRIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVKQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDQAARTILTDAGYPLEDPDKIKLLKKNEELLGTVIQLLSAENVHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            1665..2438
                     /locus_tag="ACG808_001741"
     CDS             1665..2438
                     /locus_tag="ACG808_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5047367.1"
                     /translation="MTLLIKQELFKLIKKKSTAVLSVLLVALLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMESQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSIYLYLLAIIVTVLMKLILFPSISFTEKISTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIALFLALGYLVFKRKNV"
     gene            complement(2615..4039)
                     /locus_tag="ACG808_001742"
     CDS             complement(2615..4039)
                     /locus_tag="ACG808_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFS5047368.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNVASIVMSTIFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFINNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGFLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            4250..4696
                     /gene="nrdI"
                     /locus_tag="ACG808_001743"
     CDS             4250..4696
                     /gene="nrdI"
                     /locus_tag="ACG808_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFS5047369.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEYEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQRFGFPFLADYELRGTPSDVTRVYQILAEKNK"
     gene            complement(4766..6748)
                     /locus_tag="ACG808_001744"
     CDS             complement(4766..6748)
                     /locus_tag="ACG808_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFS5047370.1"
                     /translation="MAKNELTLEELAKQIYQGAGGMGNVESIVHCMTRVRMKVINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQEMVDLAGVKLGEKLPSASATTAS
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAGTWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALATGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKAVPQASA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVISSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAADK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6762..7658)
                     /gene="murQ"
                     /locus_tag="ACG808_001745"
     CDS             complement(6762..7658)
                     /gene="murQ"
                     /locus_tag="ACG808_001745"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFS5047371.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELPSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISEHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEAAQAKLAEANGFVKGTL"
     gene            complement(7661..8722)
                     /locus_tag="ACG808_001746"
     CDS             complement(7661..8722)
                     /locus_tag="ACG808_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFS5047372.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQNRLIELGSEAKKNKLELMVDISGEALKRAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPNDIDELKEAAADFSQIEAWHNYYPRLETGLDKEWYQEK
                     NQWLKTQGFLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSEHVQEQFASFQKEQTVLLHTEPVDEVFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPVRNTAKRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(8934..9461)
                     /locus_tag="ACG808_001747"
     CDS             complement(8934..9461)
                     /locus_tag="ACG808_001747"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS5047373.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQALAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELISYE"
     gene            complement(9528..11030)
                     /gene="eat(A)"
                     /locus_tag="ACG808_001748"
     CDS             complement(9528..11030)
                     /gene="eat(A)"
                     /locus_tag="ACG808_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFS5047374.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            complement(11288..12409)
                     /locus_tag="ACG808_001749"
     CDS             complement(11288..12409)
                     /locus_tag="ACG808_001749"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFS5047375.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            12535..13476
                     /locus_tag="ACG808_001750"
     CDS             12535..13476
                     /locus_tag="ACG808_001750"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFS5047376.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(13619..14515)
                     /locus_tag="ACG808_001751"
     CDS             complement(13619..14515)
                     /locus_tag="ACG808_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFS5047377.1"
                     /translation="MNKNDVLLIAKNKSLYFFPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAEKYKQEFTKR
                     GVTTNYFILPLRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYQFYHSENIFLLQERDLDELPEFLHTPFQPIPSELLEFFDAENWAKR
                     FFTTDSAIYEQYEYDLSSVKNK"
BASE COUNT         4257 a         3117 c         2623 g         4663 t
ORIGIN      
        1 tctagttact gtttttttaa atgattaata aataacaact cctttctatg aaattgcaaa
       61 gcattcatct tcttttctgc taacgatata tccgtcaatg cttgttacac tttactaaaa
      121 agtatgagat tgccgatatc tggataggtg tgctatacta cagaaaaaga tatacgatct
      181 gccaccgggt agtaaaaaag aggatgatca ttccattagg tctttgaagg gatgattctc
      241 ctctttttgt gtatttttta tattgaagga ggaacgtatg tatggcatgg atggaattaa
      301 tcatagcagg aattttggaa gtattctggt caacgatgat gaaatggagt gatggttttt
      361 ctaaaatcaa ttattctttt tacacagtga ttggaatgat tgctagtttt tactttttgt
      421 caaaggcaat caaaagtttg cctatgagtc tctcttatcc tatttggaca gggattggcg
      481 cagttggttc ggtcattatt ggtgtggtct tgtttcatga taagctgaat ctttccactt
      541 ggttctttgt cgggttgctg ctcatcagca tcattggcat caaaatcact agcggtcact
      601 agatacttag acaagcagac aaggactgat cagaaaaagc ggggcttttt atttgatatt
      661 cctctttcta tgaaaaaaca agaaaataag actaatgacc tagcgacttc tctactcatc
      721 aggtgtatgc ttgtactatc tatagaaaag gagtgaatat ttttgaaaag tctttcggtc
      781 caagaattat ccaagaactt tggtcacaaa caagccctca gcgatgtcag ttttagctta
      841 tctcctggca aaatcgttgg attagtaggt cctaatggcg ctggaaaaac aacgatcatg
      901 aaagctattt taggactgat ccattactca gaaggaacga tccgtattgg tgaaaatgag
      961 atttctccct ctagtcataa aggattagaa caagtaggag cattgatcga atatccgggt
     1021 atctatccat tcttaacagg ttatgatcat ctgaagttat tctctgaaac aaacgatgta
     1081 tctgctatcg acactatcgt gaaacagctc aagatggaaa aatacattca caaaaaagcg
     1141 aaatcctact cgctgggaat gaaacaaaaa ttgggaattg ctcttgcctt actgaaccat
     1201 ccgtctatcg ttattttaga tgagccgatg aacggtttgg atccacaagc aacaagagat
     1261 gtgcgaaaaa tcattaccga cttggctact cagggcacct ctttcttgat ctccagtcac
     1321 ttactcagcg agcttgaaaa aatggtcgat gagctgatcc tgatcaatca aggaaaaatc
     1381 atcaaacaat gtacaatggc tgaattgaac caaacagcgc aagatttcat cgttatctct
     1441 acgacaaatg atcaagctgc tcgaacgatc ttgacagacg ctggatatcc cttggaagat
     1501 cctgataaaa tcaagcttct aaaaaaaaac gaagagttat taggaactgt tatccagtta
     1561 ttgtctgcag aaaatgtcca tgtgacagat gtccagcatc tgacgaatga tttggaatct
     1621 tccctattgg aaattctgga ttcagtaaag ggggaagaag aaaaatgacg ttgcttataa
     1681 aacaagaatt atttaaattg atcaagaaaa agtcaactgc tgttttatct gttctattag
     1741 ttgctttgct cataggaaca gcacttctag ccaaaaaata cacgacgatc attgatccag
     1801 ttgaaatgac tgctcagctt ttcagtgcca catcttggat tgtctttatc atgattgctg
     1861 ctgcaagtac gatcatttca atggaatcac aatatggtac attgaaaaat ctcctttatc
     1921 gaaagtattc tcgaggagaa atattagtaa gtaaatggat cacattagtt atttattcta
     1981 tttatttata ccttttagct attattgtca ccgtgctgat gaaactgatc cttttccctt
     2041 cgatttcctt tacggagaag atttctactg gtcagacact gatccaaagc ttattcactt
     2101 atactttggg tagttatatc ggtttatggc taattttaag tttagtgttg atgattgctt
     2161 gttttatcaa tagttccggt gcctccatct cagcagggat cgtcttttat tttgcttcat
     2221 caatcatatc tggtatcctc atcgcactca ttcaaaaatg ggagtggatc aaatggaatc
     2281 caatcagtat gctgaatctg caaaaccaaa ttggcaacga agagattatg aagcaattga
     2341 ctcatctttc tactaatcaa atgctgtttg gcaatttagc ttacattgct ttgttcttag
     2401 cattgggata cctcgtattc aagcgaaaaa atgtatgatg gttcattaac aaagaagttg
     2461 tgacagaagt ggccagtttc aagaaatttc ggcttatttt tagaagaagc tacttcttga
     2521 acaccattta ttcaaaaaaa gaagcgatga cacgaatgtt gtcaccgctt cttttttgaa
     2581 taaaccacag gcttcacagc ctctctttat acttctatac gtgaagcatc ccagtggcaa
     2641 ataaaaccat tgcgattact ccgaccatcg tcacaccgat aagtaaaatc ttatctaagt
     2701 ggatatgttg tacaggttcg ttttgttctt tacgtgcttt gaggtaaaaa taaatacctg
     2761 gtaagaaaag aacaagaaca agcatcactt cattaaaggc tacaactagc atggcccata
     2821 cttgaaaagc acttgctaaa aaccctacta aaaattggac ccactctttc cgctgataag
     2881 aaagcttcat ttggtacagc cctaagaaaa gccacgtgaa gaaaatcgct gctgtaccaa
     2941 gtgtataagc aaagttatat gcactgtcag tgaataataa tgaaagtaaa aagacattcg
     3001 tacataacgt cgtcaatacg atagcataag ttggtgcccc atttttgttg agttttcccc
     3061 atgctttagg caaaagtccg tcctcagcca tattccccat tgtttcaatc ggcaagattg
     3121 tccatgagag ccaaacccca actaaagaaa tgatcaaacc gccgttgatc acgaccgccc
     3181 cccatttacc aaccatcatt tcaaatacat atcccatagc aggttgcgct ttgatcgttt
     3241 gcagctgatt catcgtcatt gtgccatatg gaagcatgga aaccaagacg taaataatga
     3301 tcaaaccgaa tattcctaga atcgttgctt tcccaacatc cgattgtttt tttgctcgtg
     3361 aacctacgac agaagctcct tcaataccaa cgaataccca gagcatcacc atgatgcaac
     3421 tcaatacttg actagaaaac gggatcggct gcccattcgc attcaatatg atatcgctag
     3481 aaaagttatt aatgaattgt tggatcgaaa aggtgctgat agctactaca ataaataaaa
     3541 ataatggcac taatttacaa aatgtgacga tagcattgac aaagctagca ttttcaactc
     3601 cacgattcac tagccatgca tatccccata agaagatcgt actcatcacg atcgatgcca
     3661 cattttgacc accttcgaat acggggaaga aataacctaa tgcactcatc aacaatgttg
     3721 cgaacgccac gtttcctaac cagcatgtga gccagtaacc ccaaccgctg ataaaaccag
     3781 cgaaacggcc aaaaccttct tctgcgtaac taaagatccc gccttttaaa tctggacgtt
     3841 ttttattgat attattgatc gataaggaga gcatcaacat tgctgttcct acgagtaccc
     3901 agctgaagat agctgggcca ccagccgatc cggagatgtc cgtcataata ccaaatacgc
     3961 cacctccaat cgctgaacca atcactaagg cagcaagtgg aatcaaacca attccttttt
     4021 tctgttgctc tgtatccatg taagcatcct ccaaagtaaa accgctttta tcaaaaagta
     4081 taacaaacct tttttatttt tacttaatta ataaaaactt taatagaaat ataattctac
     4141 cgactttttt ctcattatac aagcaaagcg cctagtccct ttccaaatta caccaccaaa
     4201 aaaacttaaa aagatgctat actaaaggga aagaattggg ggatgatcca tgaacatttt
     4261 atatatatcg atttcaggaa atacgcgtgc ttttgccaaa catctagcag aatatgctga
     4321 aaaaatgcat gcagaagatc ctgtcaatcc tgaagttact ttaaaagaaa tccatgaaaa
     4381 tagtgatttt acgaaagaaa cagaaccatt ttttactttc gttcctactt atttagacgg
     4441 cgggaatggg ttagacaacg gtgacacaga aatcttgacc gaaacgatgc gcgaatatct
     4501 ggaatacgaa gacaatcata aactttgcct tggtgtagtc ggtagcggta ataaaaactt
     4561 caacaatcaa tattgtctga cagccaaaca atacgcacaa agattcggct ttcctttctt
     4621 ggcagattat gaattacggg gaacaccatc tgatgtgaca cgcgtttatc aaattttggc
     4681 agaaaaaaat aaataacaca aaagaagaag ctgaaaaata aatttcggct tcttcttttt
     4741 atgttctcta ttgtttctat tcttattaag ctttgacatc catcacaggt gcttttgctt
     4801 tcgctttacc tgttttttcc aagacaaagt tttttacacg atccatattg gttaccaaga
     4861 cgatcatgga ggtttctttg tctgctgctt tgattgcttc taagtcagct tctgctacaa
     4921 gcgtatcagg tgtcacttgc tgtccttctg ttactttcac atcgaatggc gtaccgccta
     4981 agtcaactgt gttgatgccc atgtgaagca agatttctaa gccatttggt gtcgtaatgc
     5041 cgatagcatg ttttgttggg aaaacagaac ttattactcc agtaaccggg gaatagatat
     5101 gtccgttttc tggttctaca aagtaacctt cacccatcat tttttgtgca aaaactgggt
     5161 cacttgcttt atctaaagaa acaactgttc cgtcagcaac tgatgataag atagtcacac
     5221 tttcagctga agcttgaggt actgcttttt caggtacttc atttactgtg gctgtttcgg
     5281 tcgctagttc ttctttttct aaacggtctt taggaatacc gaagaagaat gtagcaacga
     5341 agccaccagc gtaagcagcc aataaaccaa ggatatagcc taaccattta ccatcagaga
     5401 tcaatggaat caaagcggct cctgaaggtc caatagcgat tgctccgaca tttccgatca
     5461 ttccgatcac tgccccaccg atacctccac cgatacaagc agtaataaat ggacgtccta
     5521 gaggtaatgt tactccgtaa atcaatggtt cgccgattcc taagattccg actggtaaag
     5581 cccctttgat catttctgtt aattctttgt cttttttgca gcggatccat aatgccaatg
     5641 ctgcacccac ttgtcctgct ccggccatcg caaggattgg aagcaataag gtacgaccag
     5701 tttcatcaat catttgtaca tggattggcg tcaagatctg atgtagtccg aacatgacca
     5761 ttggcaagaa taatgtacct agaacgaagc cggcaaatgc tcctccaaca ccaagtaccc
     5821 agttgatccc accgaccatg ccgtcagaaa tgaaccctgc taaaggcatg atgaagaaaa
     5881 tttcgatcag accgatggca atcagggaaa gagttggcgt aacgatgata tctaccgcat
     5941 caggtactac tttatgcagt tttttctcaa cgattgacaa taaccaaact gcgaagataa
     6001 cacctaagat cccgccttgt ccagttgcaa gagcttcacc ggtaaacaag tttttcagtg
     6061 gtgcttctgg attcatccct gtcagcatca caacagcccc gataactcca ccaagcgttg
     6121 gcgttgcacc aaaaacttgt gcagcattga tacctgtata gataactaga tagctaaaca
     6181 tcccgttttt caagatgttc atcacatcga tatattgttg ccatgttcct gcgtctagtg
     6241 ttccagctgt gactaagttt gctaaaactg cggcaatccc tgccacgatc ccagtgccga
     6301 caaatgcagg gatcattggt acaaagatat tagagatgtc ttttaatacc gatttgattt
     6361 tagagggttt ttgtttcgat ttttgtgccg ctttcatttc ttgtgcccgt tcgtttacac
     6421 ggtcttttcc agatgcagaa gaacttgctg tagttgcact agcagaagga agtttttctc
     6481 ctaatttcac gcctgctaag tcaaccattt cctgcgctac tttgttcact ttcccaggtc
     6541 cgatgatgac ttggagctgt tcatcctcta ctacgccaag tactcctgga atcgctttta
     6601 attccgagac atttacaaga tcatcattga tgaccttcat acgtacacga gtcatgcagt
     6661 ggacaatgct ttcaacattg cccataccgc ctgctccttg ataaatttgt tttgccagtt
     6721 cttccaaagt caattcgttt tttgccatgg tcttttcccc ctcataaggt tccttttaca
     6781 aaaccgtttg cttcagctaa ttttgcttga gctgcctctt tatcactatt tgtcaaaatc
     6841 atgacgatag ccagtttgac attttgatct gcttcctcaa agtacttttc agctgtttcg
     6901 tagtcagctt ctgtcgcttg catgatgatt cgttttgagc gttcaattag tttttcattt
     6961 gttggtttga cgtcgaccat caagttattg aatactttac cgataccgat catactgata
     7021 gtagaaatca tattcaaaat cagtttttgt gctgttcctg acttcaatcg agtagagcct
     7081 gtcaaaaatt ctggtcctgc atcgacttcg attgggaaat ccgcatgctc gctgatttct
     7141 gcttgtttat tgcaagaaat cgttcctgta acagcaccaa tcgattttgc gtaatctaat
     7201 ccaccaatca catatggtgt acgaccgcta gctgcgatac caataactat atcatttttt
     7261 gttaaattca gcttgattag atcttcttgc cctaattcat aagaatcttc ggctccttcg
     7321 acggctacag tcattgcttc ttctccacca gcaattaggc ctatgaccat ttccggttct
     7381 acaccgaatg tcggtacaca ttcagcagca tctaatacac caagtctacc actcgttcca
     7441 gctcccatat aaatcaagcg accacctttt ttaaaggatt caatcgtgtt ggcgatcact
     7501 ggctcgatac ttggcagttc tttttcaaca gcagcggcca ctttctgatc ttctttgttc
     7561 atgagttgga ctgcttgaga aatactcatt tcatctaatc caaaagtttc ttggtttctg
     7621 cgttccgttg ttaggttctc taaattgatt ttgctcattt ttatcttcct tcttttctta
     7681 taaattgata gttcatacca gctttgattt ggttgatcag cggtagttct tctttgatga
     7741 ctttggctac tacattcact ttttcatccg ctggcaaatc atatttagtt aattggatct
     7801 cacccatata gcgcaaatac ttttcgttat ctaaagtaat agatcctttc atacgcttag
     7861 ctgtatttct tactgggatt ggcggaatct ctcgaaaacg cgcatctgcg cttcgtatga
     7921 catctcttgc atcatcttgc cgattcgtat gttttcctaa tatatattcg taaaaaactt
     7981 catctacagg ttccgtatgg agcaagactg tctgttcttt ttggaaagaa gcaaattgct
     8041 cctgaacgtg ttctgacaga cctgcatctc cgatataaac aatatccgtg ttacttgcta
     8101 atagatcaag agcacctgcc agaggatgga tccctcgatg ttcttccaaa gttggtaatc
     8161 cttgatacag tggacctctc aactctgtat ctcctggtac aaagccttgt acaaggaatc
     8221 cttgagtttt gagccattga tttttttcct gataccattc tttgtccaac cctgtttcaa
     8281 gacgtggata ataattatgc caagcttcaa tctgtgagaa atcagccgct gcttctttca
     8341 actcatcgat atcattaggc gtgatcgtgc tggcattcaa actgattttc aattgatgtg
     8401 accagtcggc gatctgctga ttagaaatat gataatccat tcttaaccct gttacaccaa
     8461 ttgctttcaa gggtgttaga tcttctaatg aaaagccagc tcgtttcaat gcctctcctg
     8521 aaatatcgac catcaactct agtttattct ttttcgcttc gcttcctagt tcgatcaatc
     8581 gattttgata agccgaaaca tcatcttccg gtatatgcat ggaagtgaaa ataccgacaa
     8641 agccattgga tgccatttgg cggatatact gtttcttctc ttctgacaaa gaatcattca
     8701 taaatattga aaagccaaac atattatccc tctttttctg tatccgtctt catttctatg
     8761 actagagtat atatctaaag aaacaaaatt tcaatacttt tgttaaaata attaaatttt
     8821 atttctaaaa attggattta taatgccaaa agaattgaca cagccacaaa aaaggcactg
     8881 ctaagcaaaa gcagtgcctt gaaacatttt atttaaataa cacaaaagtc taattactca
     8941 taagaaatta attctgtatt gtctaccatg aatgtaaaga acccttcatt aggcattgga
     9001 tgaaaataag cattgatcac tcttatcaag gcattatgac agactaacaa gtagacattt
     9061 tcttcgtctt ccatgcattc tttcaataaa ggaacgatcc gtgcgtacac atctaagacc
     9121 gactctccat ttgggaaacg tacagcaaac gctaaacgcg ctttttgaaa gttttcatct
     9181 ttgcttggca gtccgtcata atccccaaaa tccatttcta ccaatcgctc atcagcggtc
     9241 atcggcaaat tgagacgatc aactactgct tgtgctgtat ctcttgctcg ttgaagaggg
     9301 gaatggatga ttttcgtgat tggaacaggc aaatctacta ccttttctgc tagtgcctgt
     9361 gcctgcatct gtcctttttc agtcagcggc acatctgctc gcccgcaaac taatccttgt
     9421 gcgttccaag ttgtttctcc gtgtctcgtt acataaagca tgtgattctc tccttcgttg
     9481 acaaagttct ccttaaccat accttgttgt ctttgagatt acaaggttta gattttctca
     9541 agagaaataa tctcagtaga tactttatct agaaaggttt gatcatgttc cactagtaac
     9601 atagcaggtt tcacgttcaa aatcagttgt tctaattgtt cttgattgaa gacatccaaa
     9661 taattcaatg gttcatccca tatatagagc tcagctggtt gggataaaga tttagccaat
     9721 tccacttttt tccgttgtcc catactcatc tgttcgattt tattatgaaa aacatctctt
     9781 tccatcccaa gctttcggag attattcaaa aatgcttgat agtctactcg gtttttcttc
     9841 gcgaattccg ccaatgtccc tcgattgtct tcataatttt ggcgtacgta actaatggtc
     9901 aaatgtttgg gctgggattt ttctcctaca accttgccgt tgaatgtccc cagaagataa
     9961 tggataatgg atgactttcc cgcgccgtta ggacctgaaa ttgccatccg ctgatgaggt
    10021 tcgatcgtaa aatgaactgg ctcgaataac agattttcat accctaattg aagattttct
    10081 acgcttaaca gccgtttatg atgagacact tggctattca tcgtcaaagc atcgatatac
    10141 tcgatatctt ttaataattt ttctttctca ctgatctggg tttccatccg attcacgatt
    10201 gctttagatc gtttcatcgt ccgtgcagca tcagcgccta ctgctccttt attcactcgc
    10261 ctagattccg tatcgatgaa tccgactttt ttctttgttt tgtccccttc tcgggaacga
    10321 gaccattctg ctttttcagc tgctgttttc tttagcctgc tgacttcttt tttcaatttt
    10381 tcatttttag ctatttcaaa ttcatcacga agttttttct gttcttcata gatagagaaa
    10441 ttcccttgat aaagttccag ttggctcttt tcgattgcta aaacatggtc cactacttca
    10501 tcgataaatc cccgatcatg actcaccacg atgaatcctt gttttttctt tttcaagtaa
    10561 gccgctactt gttttctgcc agagatatcc aaatgattcg ttggttcatc gattaacggg
    10621 aaatgagtgt catccacaaa taagagtgcc agtaggactt ttgtcttctc tcctcctgat
    10681 agtgtgctga attctctcca taagatttca ggatctgttt gcatcagttg gagctctctt
    10741 tcaatttccc atatctcaaa atccataatc tcatttaaca cataataggt caaacgctct
    10801 ttatcttttg tttgctgcgg gaaatagaca aacgcttgct gatggatcac ttgcccttga
    10861 taagggagtt tgttttgcag gatattcagt aaagttgtct ttcctcgacc gtttcgtccg
    10921 ataagtccta gtttccattg tgtgtcaaag tttagattgg cttgttcaaa taataatgtg
    10981 ccttggctgt catagccgaa tgtcagattt tttatttcga ttttagacat tattccatca
    11041 cctcgtaaaa aaagaccagt cctattttca gaactggcta aagaggatgc aagggttggg
    11101 gcacacaaaa agacccatac atcgaaagaa ccaagccaat cccgaaaatc tgcacactgc
    11161 tcccttgtct ccacaaggta cagtctgaat gcagctttca tcaatcgact taatttttca
    11221 atgcacggat ccctcacttt gtcctatatt tgaaattacg atagcataaa gaaattgttt
    11281 ttgtcaacta cttcgggaga atgttgaaaa atctgctgag tgtctgtcct aagatttctg
    11341 ttttttctaa tgtttgcctg tgttccattg cttcctctaa atcgattggt cctaattgaa
    11401 tgcttaagac aatggggaga aaatcaaaga gtccttccag ctctcggtct ctacttccgc
    11461 aaatagcgat tattggtttt cgatattttt ttgcaagcac tgctacgcca tatggaactt
    11521 ttccgtggat tgtttgctga tcaatccgcc cttctcctgt aatgatgaca tccgcttgtt
    11581 cgatctgttt ttctaatcca gtgatttggc tgaccaactc aaaaccagag accatcttcg
    11641 ctcctaacaa aaaaagggct ccgccaatac cgcctgctgc tccgcttcct gggatgtcat
    11701 ctaaatccac ctcccaaaca tcttttatct gctgagcaat cttttcagct tgtttatcca
    11761 agaaagctaa cgtcgtatcc gttcctcctt tttgtcttcc aaattgccaa gcagctcctt
    11821 gttgacctgt atatgggttg gtcacgtctg cagcgatgat cacttttact ttagaaacaa
    11881 gaacgttcct tcgatccaaa tgaagttttc tagtagataa caaaggattt ccttctgtgt
    11941 acccttctaa agaagcaccc aaagattgga gtaatcctag accaccatcg ctacatccgc
    12001 ttcctcctaa agtgacaatg atttgttcca cttcttgaag cagtgtatct ttgataagct
    12061 ctcccaaggc ataactagaa gcaagtcgga tggtttgatc cgttggttta tcaagtaaat
    12121 ctaatcctaa tacttgtgca gattcaatga ctgcggtatt ttttccttgc cagttcgtca
    12181 ataaataact gatttttcgc tgacgaaata agagatccat cccatcaatc gtacgccagg
    12241 ttcccccgag cgcgtaatag attgcttctg cagtattttc tccgccatct gcaatcggca
    12301 cattgatttt tcttccttta cggtcttccc aaccatttag aactgcctga tttaattcaa
    12361 atgaagaagc acttccttta aatgaatcga ttgccgccaa aatatccatc tatatccctc
    12421 actttcttct taagtaagac actttttttc acttttttca aacaaaacaa tgctattttc
    12481 ttctgttttt ctatatacta aaagggaact cattgaataa aggagagaaa aaacatgaaa
    12541 atcgcaatag ctggagccgg agcaatgggc agccgaatcg gattaatgtt gcatcaaagc
    12601 ggaaatgagg tcgtcttgat cgatcgctgg cctgctcaca ttgaagccat tcgtacaaat
    12661 ggattgattg ctgactttaa tggcaaagaa gtagttgcaa aactgccaat ctattcacca
    12721 gaagaaatca tcgaaagcaa caaacacgtg gatttgatcg tagccttgac aaaagcgaat
    12781 caattagatg atatgtttcg ttcgatccaa tccattatta ctgagaatac ttatgttttg
    12841 tgtttactga atggattagg tcatgaagat gtactggaaa aatatgtacc aaagaaaaat
    12901 atcttatttg ggatcacgat gtggacagct ggtctagctg gtcccggaaa agtcacatta
    12961 ttaggtgatg gagaaatcga actagagaac ttggaaccag aaggcgaagc atttacaaaa
    13021 aaagttgtag aagtctttca agaagcaaaa ttgaatccaa tctatagtca taatgttcgt
    13081 tattccattt ggcgaaaagc ttgtgtaaac ggaacattaa atggattatg cacgatattg
    13141 gattgtaata ttgctgaatt aggtgcgcaa aaagcggcag agggcatggt tcgaacgatt
    13201 gtatctgagt ttgcttctat cgcagcaaaa gaaggaatca cattagatca agaagaagtg
    13261 taccaacata ttgcttctac ttatgatcct gacaacatcg ggttgcatta cccatctatg
    13321 taccaagacc tgatcaaaaa ccatcgtttg actgaaatcg actacatcaa tggtgccgtt
    13381 tggcgaaaag gacaaaaata tgatattgct acaccatatt gtgccttttt gacccaatta
    13441 gtccatgcaa aagaaggaat ccttggcgca gaataaaagg ctctctgatg agaatcagct
    13501 ggatcagcag ctggttctca tttttttacc cccatgaaag ttcaaaaaga aaaacacaat
    13561 tctcatgttg ccataagaat tgcgcttgtt tattttcatc tatgattggt tggcactttt
    13621 atttattttt aacagaggaa agatcgtatt catattgttc ataaatagcg gagtcagtag
    13681 tgaaaaaacg ttttgcccaa ttttctgcat caaaaaattc caacagttct gatgggattg
    13741 gttgaaatgg cgtatgcaaa aattccggta attcatctaa atcacgttct tgaagcaaaa
    13801 aaatattttc agaatgataa aactgataat gagctatggc ttgattatct gtcactagtt
    13861 tcttttgaaa atacatagat tcaaacgttc gcaaagtcac tccttgctgc ccttcccgca
    13921 acagttctag aatccccctc gattccccgg tttttttcag atattcttca taagtaaggt
    13981 atccaggttg ttcatttcct cgaagcggaa gaataaagta attggtcgta acacctcttt
    14041 ttgtaaattc ctgcttatat ttttctgccc gttcttttct gccgttattt gttgcaccaa
    14101 aaaatagatc cgtctttggt tcgctagaag aaaaatccat atgtttcgaa taaaaggaac
    14161 tattatgttt tagtccaagt tctttcgctt ctaaaaaatc aaaatgataa atctcatcga
    14221 ttgccggttc atcttgtgcc tgcttcagca tcgcctcgtg ttctgcttta aagtggttcc
    14281 aaaaaaataa cacgattttt gcattggtaa atggacgcac gcgtcgagcc atctccatat
    14341 caaatccata atctaaaaaa acgatcagct gataatttgc caactgttgt cgagctgatt
    14401 taatgaattc cgactgtagt aaatcttttc cggaccaaac atctaaagag cggatggaag
    14461 gaaaaaaata aagtgattta tttttcgcaa ttagcaatac gtcgtttttg ttcatctgtt
    14521 ccgcctcact ttctctgtca ctttttccta tgatagcctg tttaaatagt aaaaagccag
    14581 taaaacgctt cctattattt ttataaaaat ttaacatttc tatgaaaata cgatgaaata
    14641 aatcattctt ccattaaaat
//
LOCUS       DAZUMK010000062        14564 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000062 DAZUMK010000000
VERSION     DAZUMK010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.063"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(313..1008)
                     /locus_tag="ACG808_001752"
     CDS             complement(313..1008)
                     /locus_tag="ACG808_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFS5047378.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(1098..2198)
                     /gene="ychF"
                     /locus_tag="ACG808_001753"
     CDS             complement(1098..2198)
                     /gene="ychF"
                     /locus_tag="ACG808_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFS5047379.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(2207..2398)
                     /locus_tag="ACG808_001754"
     CDS             complement(2207..2398)
                     /locus_tag="ACG808_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFS5047380.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(2419..3309)
                     /locus_tag="ACG808_001755"
     CDS             complement(2419..3309)
                     /locus_tag="ACG808_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFS5047381.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(3296..4063)
                     /locus_tag="ACG808_001756"
     CDS             complement(3296..4063)
                     /locus_tag="ACG808_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFS5047382.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(4075..4788)
                     /gene="rsmG"
                     /locus_tag="ACG808_001757"
     CDS             complement(4075..4788)
                     /gene="rsmG"
                     /locus_tag="ACG808_001757"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFS5047383.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(5020..6921)
                     /gene="mnmG"
                     /locus_tag="ACG808_001758"
     CDS             complement(5020..6921)
                     /gene="mnmG"
                     /locus_tag="ACG808_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFS5047384.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(6938..8335)
                     /gene="mnmE"
                     /locus_tag="ACG808_001759"
     CDS             complement(6938..8335)
                     /gene="mnmE"
                     /locus_tag="ACG808_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFS5047385.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            8634..9017
                     /locus_tag="ACG808_001760"
     CDS             8634..9017
                     /locus_tag="ACG808_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047386.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(9185..9943)
                     /gene="jag"
                     /locus_tag="ACG808_001761"
     CDS             complement(9185..9943)
                     /gene="jag"
                     /locus_tag="ACG808_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFS5047387.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(9961..10779)
                     /locus_tag="ACG808_001762"
     CDS             complement(9961..10779)
                     /locus_tag="ACG808_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFS5047388.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(10776..11132)
                     /gene="rnpA"
                     /locus_tag="ACG808_001763"
     CDS             complement(10776..11132)
                     /gene="rnpA"
                     /locus_tag="ACG808_001763"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFS5047389.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(11250..11384)
                     /gene="rpmH"
                     /locus_tag="ACG808_001764"
     CDS             complement(11250..11384)
                     /gene="rpmH"
                     /locus_tag="ACG808_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFS5047390.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            11907..13241
                     /gene="dnaA"
                     /locus_tag="ACG808_001765"
     CDS             11907..13241
                     /gene="dnaA"
                     /locus_tag="ACG808_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFS5047391.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            13439..>14564
                     /gene="dnaN"
                     /locus_tag="ACG808_001766"
     CDS             13439..>14564
                     /gene="dnaN"
                     /locus_tag="ACG808_001766"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFS5047392.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRT"
BASE COUNT         4028 a         3070 c         2621 g         4845 t
ORIGIN      
        1 gcttctcatg tttttgaatt ggttgggttt attaccgcag atgcaaactt ttgaacaccg
       61 tttagtggag aggctgtgaa aaagctgctt agtttcgagt agtaagaaaa attttgtgaa
      121 aatggctttc cacatttttg cataaattgg acttaatacc gagaaactgg cttttgaaca
      181 ccgtttatcg taaaaaggga ctgtgacaag acttttgtca cagtccctta ctattattta
      241 actatggata aacaaaatga ggttctttac tacccctaga tatctagcaa aatagctgat
      301 ctttttgcta gtttatcttc ttgggacgga taaacttcca ataatcccgt attttttctt
      361 caagtagtaa cggattgcta aagcagttgc cccgataatg ataactacta ctggatctaa
      421 tactggattg ataactggtg gcaatagtgt tgtcgcagaa aacagtacga cccaaagaag
      481 tgtcgttcca cctaggataa gcaatgtctt acccattcct ggtttcttgc ttcgatctgc
      541 tccgggatat tcatattgat agatgtattt gtacatcata taaaagaccc agccaccgat
      601 tgcagaacct agaacgagtg tagaaaagcc ataagctggc gttgttccac gtgaaaacaa
      661 tccggtaatc gcaatcatga tacttaatag accaagcaac agcaacgtat tatctaacca
      721 catcaataaa ggggtggatg aattttttac ttctgctggc ttattgatga ttgcttccgt
      781 tctttcagag actgtcccaa acaattgacg ggctgttctt ccgccttttt gttctgcaac
      841 taaagtaggc agaatgtcat gaagggcttt tactttttct tcttcagaca aattcgctgc
      901 ttctaaagat tttttcaaat caaaaatgta ctgttgattt cttttggtta attttgtttc
      961 taaactattg ttttctttta cgattgctcg taattgttct ggttccatta tctaatagtc
     1021 ctccttagcc gttccttttc tcaacggtcg tcacaaaaga cattctacca tattctacag
     1081 ctagtttgat agccctttta cacattaaag cggaataaca ttacgtcgcc gtcttgtacg
     1141 acataatctt ttccttctaa tcgaacacgt ccagcttctt ttgctgcatg catgtttccg
     1201 taatgatcca agtcttcaaa agagacagtt tctgcacgga taaatccgcg ttcgaaatct
     1261 gaatggatga ttcctgctgc ttgtggtgct ttgattcctt ttttgaatgt ccaagcacgt
     1321 acttcttgtt ccccagcagt aaagtaagta gctaagccta acaaatcata agcagcacgg
     1381 atcaactgat ccaaacctga ttcttcgatt cctaaagctt ctaaaaattc cgctttgtct
     1441 tcttcatcta attctgcgat ttcttcttct gcacgagcac tgaccacgat cacttctgca
     1501 ttttcttctg cagcaaagtt gcgcacttct tgtacataat gattcgcgtc tgcatctgct
     1561 acgtcatctt ctgagacatt ggctacataa agaattggtt ttgtcgtcaa taaaaatagt
     1621 gatttaacga ttttttgttc ttcttcagta aattcgatcg ttcgggcaga ttttccttct
     1681 tccagtacag gtttgatttt atccagtaca gctagttctg ccactgcatc tttatctttg
     1741 gttttagcaa cttttgctac acgagtatag cgtttgttga tagattctaa gtctgctaac
     1801 actagttcca agttgatcgt atcaatatct tctagtggat caacacgtcc ttctacgtga
     1861 gtaatattat catcatcaaa acagcgaaca acgtgacaaa tagcgtctac ttggcggata
     1921 tggcttaaga attgatttcc caaaccttct cctttacttg cgccttttac gattccagca
     1981 atatctgtaa attcaaaagt cgtcggaaca gtttttttcg gatgtacaag ttctgttaag
     2041 cgttgcaaac gccagtcagg tacttctacc atccctacgt tggggtcgat cgtcgcaaag
     2101 ggatagtttg ctgcttctgc tcctgctttt gtgatcgcat taaacaaagt tgattttcct
     2161 acgttgggca acccaacaat tccagcagtc aatgccataa atttattcac tctttctctg
     2221 catttttttc aattactttt tttaatttct tttcaaaatc tcgacgggtc atcatgacga
     2281 tatgtccgca attcatacat ctgattttga tatctgcgcc cattcgaatg acttcccagc
     2341 gattcgtctg gcaagcgtga ggttttttca tttcaacgat atcgtgcaag tcgtacataa
     2401 aaatagcctc ctttttgttt attcttcatc aaattgaata ttcaagatat caagaattcg
     2461 agtcaaatct ttttgagaaa gatattcaat ctcgatctta cctttcccat ttttttcttg
     2521 gatagctaca cttgtaccaa atttatccat caaacgatct tcgctttctc ggatataata
     2581 tggtttttcc tttgctagcc ttgggatttt tttcttttct tttccatcat tttgatttaa
     2641 ctcagaaaca agctgttcca gttggcggac agttaaattt tcagttactg cacgattggc
     2701 taatttcaag atcaatgttt tattttttaa acctaacagc gtacgggctt gtcccataga
     2761 taaacgctga tcttgtacca tttccttgac aagatccggt aaagaaagca agcgcaaata
     2821 attggcgata taaggtctgc tctttcctaa acgactagct acttctgctt gtgttagttt
     2881 caggtttttc atcagcatct catatgcttc tgcttcttct aacggattca gatcttctcg
     2941 ctgcaagttt tccagcactg ctacttgcat catcgcttct tcgtcaaagt ctctaacgat
     3001 agcagggatc gtttcttttt cagctagttt ggacgctcga aaacgtcgct cccctgcaat
     3061 aatctcatat ccttttactg cggaacggcg tacgatgatg ggttgaaaga cacctgattg
     3121 ttcaatagaa ttagctagtt cctgcaaaga tacttcttca aatgttttac ggggctgata
     3181 cggatttggt cgcagctcat tcaatggtag ctggacaacg gtatcatctt ttacatccac
     3241 cgtctctaag ttttctaaat cttgaaacag tgcatcgatt cctcggccta atcctttact
     3301 tttcttcacg acttaccact tcctttgcca atgcttggta gacttcagct cctcgagaac
     3361 gtggatcgta gtcgatgatc ggttttccat gactaggtgc ttctgataaa cgaatatttc
     3421 ttggaatgat cgtttcgtaa actttttcgc ggaagtattt acgtacttct tctacgactt
     3481 catttcccag atttgttcgt gcatcataca ttgtcagtaa gactccctcg atctctaatt
     3541 ctggattaaa gtgtttttgg actaaacgaa ccgtattcag taattggctt aatccttcca
     3601 atgcgtaata ctcacactga acaggtatca aaatcgaatc acttgctgta aatgaattga
     3661 ttgtcagatg gccaagagaa ggcgggcaat cgatcaaaat ataatcatac tgactgctta
     3721 cttctgctaa agatcctttt aatcttgatt ctcttgccat cattgacgtc aattcgatct
     3781 ctgctcctgc taattggaga gtcgcaggca caatactcaa attttcgtgt tcagtgatca
     3841 aagttgcctc atcaattggc aattcattga ctagaacatc atagatgtct cttgtcacat
     3901 caggttttct gatcccgaca ccactggtag catttccttg tgcatccata tccactagca
     3961 atactttttt gccaaggcta gcaagacaag cacctagatt caccgtcgtc gttgttttgc
     4021 cgacgccacc tttttggttc gcaacagata ttatttgtgc catctcttct cctcctattt
     4081 gattggctgc ttattaggca gtccgggctt tcttgggtat ttctttgggg ttggtttctt
     4141 tttttgaata acgatgatat gccgttgatc agagacattc ggcaaggtaa ccgcctcttc
     4201 tttgaggaat ttaccaccta ataaagcaat cgcttgtttt gcttctgtca attcctcttc
     4261 actttttgct gctttcaatg cataaaaata gccttcttgt ttagccaagg gcaaacacaa
     4321 ttccgctaac acattcaaac gagcaaccgc acgagcagtg acaaggtcaa atgatgcacg
     4381 aaagtgagga ttttgcccaa atgtttctgc ccggtcatga tataaggaag catcgattcc
     4441 taataatcgg gttaactcag ataagaaggt tattcgttta ttcagagaat caacgatcgt
     4501 tatctgcagt tctggaaagg caatttttaa aggtatgcta ggaaaccctg cccccgaacc
     4561 aacatcgcat aaagataaag gttgcgtaaa gtctacttca aatgctaatg taagtgaatc
     4621 gtaaaaatgc ttcagatata cgtctttttc atccgtaata gctgtaaggt tcatcttttc
     4681 gttccattcg actaatgtcc gataataaat agcgaattga ttcatttgat gatcggtaag
     4741 ctctatccct ttttctgcca aggattggcg aaattcttct ggtatcataa atattccttt
     4801 cattgtgaaa cagcaattgt ttcacagatt ctctccctac tttaacgcaa aatctctaga
     4861 catgcaatcc ttccatatgg attcttaaaa atttgaaaag taagaaaagc cgatagactt
     4921 tttctttcta gtatgactca taaaatatac acagaagaga gaactagctt tgagcaattt
     4981 ctgcaaaaac tagttctctc ttctatttat tattacgtat tacgctggtt cactgacttt
     5041 cgcaatcttc ccttgttcga tatagaccat taggatactt aggtctgcag ggtttacgcc
     5101 actgattcgg ctagcttggg caattgtttc tggttggatt ttctgcaatt tttgttttgc
     5161 ttctgttgct aatccgttga ttgctgcgta gtcgatgttt tctggaatct ttttcgcttc
     5221 catgcgtttt aatttttcta ctttttccat ggcttttttg atgtagcctt catatttgat
     5281 ttggatttct acttgttcga tttcttttcg tgtcaattct tcattttctg gaataaaccg
     5341 tagaagttct gcataagtaa tctcaggacg tcttaaaaag tcgcttgcta gtacaccatc
     5401 ttttaatggt gccgcattaa tcgttgctaa aaagtcttgg acttcttctg ttggtttgat
     5461 tcgtacattc atcaaacgtt tgatttcttg ttcaactgct tcttttttct tcaaataatt
     5521 cttgtattga tcttctttta ctaagcctac acggtgaccc atttctgtta atcgcaaatc
     5581 tgcattatca tgacgtaaaa gcagacggta ttcagcacgt gaagtcagca aacgataagg
     5641 ttcgttcgtt ccttttgtta ccaaatcatc gatcatgaca ccgatgtaac cgtcacttcg
     5701 tttgatcact aaaggttctt ttccctgaac tttcaaagct gcattgatac cggcaatcag
     5761 cccttgtcca gccgcttctt cataaccgct tgtaccattt gtttgtccag ctgtataaag
     5821 attttcaacg actttagttt ctaatgtcgg acggagctgg tgtggctcta ctacgtcata
     5881 ttcgatagca tatcctgtac gcatcatttc tactttttct aatccagcaa tagaatgaag
     5941 catgttgact tgaacatcct ctggtaaaga agtagataaa ccttgaacat agacttcttc
     6001 cgtatttaag ccttctggtt ccaaaaataa ttgatgacgt ggtttgtctg caaaacgcac
     6061 gatcttatct tcgatggatg gacaatagcg agcgcctacc ccttcaacga tccctgtaaa
     6121 cattggggca cgatgtaagt tgtctcgaat aatttggtga gtcgtctcac ctgtataggt
     6181 cagccagcaa gattcctgat ctagtttata ggcactatct ggtgtgctga agctaaaatg
     6241 attcggctct ttgtcaccag gctgctcttc tgtaacagaa tagtcgatcg tacttgattt
     6301 tactcgaggc ggtgttcctg ttttgaaccg ttcgatagca aagccaagtt cttttagatg
     6361 attcgctagt ccaatagaag gttgagagtt attcggacct gatgaatatt tcaattcacc
     6421 gataatgatt tctccgcgaa gtgctgttcc ggctgtaata acaactgctt tggcccggta
     6481 gctcgctcct gtggacgtca ctactccatg acaaacacca tcttcgacga tcagttcttc
     6541 gacgattcct tgtcttagcg tcaaatttgg ttctttttcg atcgtatgtt tcatttctgt
     6601 tgaataagca tgtttgtctg cttgtgctcg caatgctcgg acagctgggc cttttcctgt
     6661 gttcaacata cgcatttgga tataggtttt atcgatattc ttacccattt ctccgcctaa
     6721 cgcatcgatt tctcgaacga caacgccttt agctggtccg cctacagaag gattacatgg
     6781 catgaatgct accatgtcca aatttattgt cagtaataat gtttttgatc ccatacgtgc
     6841 agcagcaagt gcagcttctg aaccggcgtg tcctgcgcca acaacgatca cgtcatacgc
     6901 atctgcttca taatgttcca atcgtttgtt cctcctttta ttttcctaaa caaaattgac
     6961 tgaataattg agtgattagc tcatcttgta cactgtcgcc gacaacttct cctagatagt
     7021 cccagcaacg cgtcatatct atttgaacga gatccacagg cattcctgaa tcaatacctg
     7081 caatgacttc ttcaagtgag agggaagctt tttctaacaa cgcgatatgg cgggtattag
     7141 aaacataagt agcgtctctt tctcctgttt ctccgctgaa gaataaatca gaaatagctg
     7201 attccaaacg atctagtccg tcgtttttag ctacggaaat tgaaaaaact ggttcatttt
     7261 cgatcaattt cttcaatttt tcttgttcca gctgtgctgg caaatctgtt ttatttagaa
     7321 gaatgatccg tttcaatcca cttgtcgctt ccaataattg ttcatcttct gctgtcagtg
     7381 gttcgctttg gtttagaaca agtaagatca agtctgcttc tgctaatgct tttcggcttc
     7441 tttcgacccc gatccgttcc accacatctt ctgtttcccg aattccagct gtatcgatta
     7501 gcttgagcgg taccccccga acattgacat actcttcgat tacatctcga gtcgttcctg
     7561 caatatccgt aacgattgct ttttcttctc ttaacaaatg gtttaacaaa cttgatttgc
     7621 cgacatttgg ccggccgatg attgccgtac ttaacccttc ccgcagaacc ttcccttgct
     7681 tggatgtagc aagtagcgct tggattcttt gttggatcat ctgcgctttt tcaagcaata
     7741 acttcgtagt caattcttcg acatcatcat attctggata atcaatattt acttctactt
     7801 gtgctaatgt ctctaaaatc tcttgtctca atgagcggat caaagcagac agattgccgt
     7861 ctaactgatt aagagctagt cccattgctt tgtctgtttt tgcccgaatc aaatccatca
     7921 cagcttctgc ctgtgaaagg tcaacacgac cattcaagaa tgctcgtttt gtgaattcgc
     7981 ctggctcagc taatcttgca ccttctctta acaatagctg caaaatttga ttgactacaa
     8041 cgatcccacc gtgacaattg atttctacta catcttcccg cgtaaatgtc tttggtgctc
     8101 tcatcacgga gaccatgact tcatcgacaa gctgattgct cttgggatca acgatatgtc
     8161 cataatggat cgtatgactt ggtacttctg ataatcgttt gtttccagat tgataaactt
     8221 tatctgctag ttgcacagct tgatcgccac ttaaacgaac gatactgatt gcaccttctc
     8281 caggtggggt agaaattgcc gcaatcgtat caaattctaa agtaatttca gccatttttt
     8341 cacttccttt ttcattttgc gcacaaaaaa agtgcccact ccacccttta tgcacaataa
     8401 acggtgaact tcgcactttg actgcttgtt cttgattaaa ttctggtcat agattatcac
     8461 acttgctata aggaatcaag tgtttaaaac aattagatga agttgagcct gttctgcttc
     8521 cattacctta tatttaattg tccttggatg tctctttgcg ttctttggtt tattcgaaga
     8581 gaagatactg tataaacaag gctttatctt tcttaaaaaa aacggtacac taaatggtag
     8641 cgctatctaa actatacact tttgaaagga gaaaatatgt gagcccgttc agatctgctg
     8701 cattggaaga agaaagtgac cctcggatca acgatgtcgt tttatctaca gggaatatca
     8761 accagaaata tatcgtccgc gatatcgttt ttgttgtgga atgcttagaa gctgatttgt
     8821 ttgacccatc ttttcccaaa gatgaccttt tcttaacaac gaagaggaaa ttaaagacga
     8881 aggtacttga atatggtgcg aatgcagtaa tcaactgtca ttttgagtat cagcaaaaaa
     8941 aacgcgacgg aaagtatttt ctggaacttt ttgcttatgg aacagtgatc caatttatcc
     9001 aaacaacgat tggttaaatg aaacagacaa aaaaagcgga tcaaaactga agacagtttc
     9061 gatccgcttt tttctatcaa agttagtgac tacttcatcg tatactctga taagaatgat
     9121 ttctagtaaa atatacttgg tttgaaaaac tttgtcaacg tgatttattc aaaaagtaat
     9181 ggaatcaata atatttttta gcaggttcta ctactaaata acgataaggt tcctcacctt
     9241 ccgaatgtgt ttttacatgt tcatctttgc ttaatgcagc atggatttgt ttacgttcaa
     9301 atgcaggcat tggttctaag aaaacaggct gccctgtccg ttttactttt tcagccgtgc
     9361 gatcagctaa acgctgaatg atctcttggc gcttttcacg gtaattgcct acattgacta
     9421 ctacagacag cttatttctt gctaaacgat ggatgaagac ttgtgccaga tattgcaatg
     9481 cattcagtgt ttttccatgt ttcccaatca gtatgccttg cttttgtgtt tctaaatgga
     9541 aaacaaccat attatttgat tctctttcca ttttgacaag agctggagca tcaagctgtt
     9601 ttgaaatttc agtaagataa actgccaatt ccgttaaagc tgcttcatca cctagctcac
     9661 ttacttcttc tgttttttct tcaagaatcg tttcgttgac tacctcgatc tcagctgttg
     9721 gttcttcttc caaaatttct tctacagtct gttctactgt ttcagaaact ttttcgctga
     9781 tacttggctg gatcgaaagc cgtgcattct ttcgacccat tcctaggaaa cctttttttc
     9841 cttcttccag tatttcgatt tcgacttggt ctttggccaa acccaaatca gccagaccta
     9901 gttggacagc ttcgtctatt gttgcagctt cataaatcgg cattagcatt acctccccta
     9961 ttatttccgt tttttcttag gattcattgc tttcttaaga gcacgttctc tttcgcggat
    10021 tctccgtgct tcttcttcac gctcttttcg aattttgaaa gggttgttga tcagcagcgt
    10081 ttgtacaact tggaaggcat tggaaactac ccagtataat gaaagaccgc tggctaaatt
    10141 gacacccatc actaaaatca ttacaggcat cacgaaattc atgatcttca ttgatgcatt
    10201 cgattcaatt tgactcatgc tagataaata cgtacttgca aaagtaaaga ttgctgctaa
    10261 aatcggcaaa ataaatgttg gatccgcgtt ccctaagttc aaccataaga aatgcccttg
    10321 tttcaactct ggtacacgtg aaatagattg ccataacgcc atcaagattg gcatttgtac
    10381 taataaagga agacaaccag catatggatt gacgccattt tctgcatata aacgctgctg
    10441 ctcttctctt aacttacttt gtgtttccgg atctcttgaa ctatattgtt gctgcagtgc
    10501 ttttaatttc ggttgtaatt cttgcgtttt acgcatgctc tttgtttgga aatgcatcaa
    10561 cggcaatagg atcacgcgga tgatcagtgt aaataaaatg atccctatcc ctgcattacc
    10621 aaatgacaac gccttgatcg cctcagcaaa gtaataaaca atataacgat cccaaattcc
    10681 tgtactctgt gcgctgactt ccccagttcc gcaaccggat aagacgatga ccaaactaac
    10741 gataccggcc atcaaaagta aacgtttgta tttcttcact tactcttttc ctctctgtcg
    10801 attatttttg caagttttaa gacgtggact aaattggagc gaacctcatc ataagtcaac
    10861 cccttcacac ttggtctagc aatcacaata aaatccaact ccggatcaat ttcttctttt
    10921 atagaataaa ctgctgctcg gatttttcgt ttgacttcat tgcggtttac cgcgttgccg
    10981 accttttttc ctacagataa accaactcga aaatgaggct gtcccggttt ttccaaacgg
    11041 taaacgacga atttgcggtt agcaaaagaa tctcccgtat ggaacacggc ttgaaactct
    11101 cgttcttttt ttactcgata cgcttttctc attagttttc tttccttttc tatctctcac
    11161 ttacgctcat aatcatacca taattacgat tgtcctttca tcatttccct aaaaaagagt
    11221 ataaaaaaaa ccactgaaac cccagtggcc taagctgaaa gaacttttct tcctttgcga
    11281 cgacggctag ctaacacacg acgtccattt ttagtactca tacgtttacg gaatccgtga
    11341 actttttgac gtttacgttt atttggttgg tatgttcttt tcatatattc cacctccaaa
    11401 atgttcttat ctattttata aacacagaca tactttgata gtataacgac aaagtagtga
    11461 ttttgtcaat ataaaataga agatcgattg ttaaaactta tccacaagat tatttttttc
    11521 tatgggaatc ctatgtggaa aaagttatgc acagggatta tccacagata ataaaaggct
    11581 ggttttacac agaaaacaca cgtgtaaaaa aagtaatcca caatcaggcg atttcctgtg
    11641 tattccgtct cctaactatt ggtatatctg tattttttat ccacagacgc aagaggaaat
    11701 ctattggttt ttacgagttt tccacctgtg cattagtggt gtggaaaact ttttttgaca
    11761 actgtgtttt tctctttttt ctttttgtcg attgtgtgga taattgtaat taaaaaattt
    11821 ttttcttcgg tttctgtgga aaactattga aaaaaatgct aaattagtac tagttattca
    11881 ctttttaatg aaggaggagg ccttatatgg tatccctcga tgctttatgg aatgaattaa
    11941 aagcaacata ccaaaaggat ttatcgcctg ctagctataa tacatggatc gaaacagcga
    12001 accctcgaac ccttgatcaa aaccagctcg ttgttgaggt tcctagcaag attcataaag
    12061 aatattggga aaaaaacctg gcgacaaaaa tcgttgaagt cggttatatg ttatcaggaa
    12121 atgagattat ccctcgcttt attactggcg aagaagcaga gcaagaagaa gttatagaag
    12181 aaaaaaatcc aaaagttgtg gcacctagtc cgctaaaaaa agccatgctg aaccctaaat
    12241 atacctttga tacgtttgtc attggtaaag gaaaccagat ggctcatgca gcagcgctcg
    12301 ttgtagcaga agatccaggt tctatttata acccgctctt cttttatgga ggcgtgggac
    12361 ttggtaagac tcacttgatg catgcaattg gtcatcaaat gctgcaaagt cagcctaatg
    12421 ccaaagtaaa atatgtaagc agtgaaactt ttgctaatga tttcatcaat tcgatccaaa
    12481 acaaaacagc agaagagttc cgtcaagaat atcgaaatgt tgatttactg ctagtggatg
    12541 acattcaatt ttttgcagaa aaagaagcta cgcaagaaga gtttttccat actttcaatg
    12601 ccttgtacaa tgaagggaaa caaatcgtac taaccagtga tcggttgcca aacgagattc
    12661 cgaaactgca agaacgcctt gtttctcgct ttgcatgggg gctgtctgtt gatattacac
    12721 cgccggatct tgaaacaaga acagcgattt tacgtaaaaa agcggcggct gaacgtctag
    12781 agatcccaga tgacacacta agctacatcg caggacaaat tgattctaac attcgtgaat
    12841 tagaaggtgc gcttgttcga gtgcaagctt ttgctgcgat gaatagtgaa gatatttcga
    12901 ctagtctagc tgcagacgca ctgaaaacgt taaaatcagg aaaaggacat cctcaattgt
    12961 caatcctgca gatccaagaa gaagtggcaa agtattatca catccaattg aaagatctca
    13021 aaggaaagaa acgcgtcaaa tctattgttg ttcctagaca aatcgctatg tatttggcac
    13081 gtgagctcac agataattct ttaccgaaaa tcggagctga attcggtggc aaggatcata
    13141 caacggttat ccatgctcat gaaaaaatcc agcagctgat ggattctagt gtcagtatgc
    13201 aaaatgaagt atcagaaatc aaaaactttt tactaaatta atttgtcgat tctatcaact
    13261 gaaaatagca tgtggataaa agaaaaaaac gttcaaaagt tatacacact ttttccacag
    13321 ctgttttttc gtttctctct tacgtttttt gagttttcca cagaatcaac atgccctatt
    13381 acttttatta ttctttttaa ctaataataa ataaacaaac attcaaagga gatacatcat
    13441 gaaagttact ttaaaccgag ctagctttat gcaggaattg caaactgttc aacgagctat
    13501 ttcaagcaaa accacgatcc ctattttgac aggtgtaaaa atcacactga cacaagaagg
    13561 tttgactttg acggggagca acgctgatat atcaattgaa acttttttgt ctgttgaaaa
    13621 cgaaaaagca aatatgcaaa tcgaatctac tggttccatt gttttacaag cacgtttctt
    13681 tagcgaaatc attcggagac ttcctgaaga aacatttact ttagaagttt tagaaaataa
    13741 acaagtagcg atcacttctg gaaaagcgaa ttttatcgta aatggattag atgcagataa
    13801 ctatcctcat cttcctgttg tcgaaagcca taaccagatg aaattacctg tacacgtatt
    13861 gactaaacta atcaacgaaa cagtttttgc tgtctcacaa catgagagtc gtccaatctt
    13921 gacaggtgtg catttcattt tatctgataa ttctttatta gctgtagcta ctgattctca
    13981 ccgtctaagt caacgcgtga ttccagtaga acaagcggct gatcattttg atattgttat
    14041 tcctggaaaa agtttgatcg aattatctcg ctcattaaca aatgaagaag aaatcgtcga
    14101 aatcagcatt atggaaaacc aagtgttgtt caaaacagaa acgatgtatt tctattcccg
    14161 tttgctagaa ggaaactatc cagataccaa tcgtttgatt ccttcaagtt ttaatacaga
    14221 ggttgaattt tctgttccta gctttttagc ggcgatcgaa cgggcttctt tactttctca
    14281 tgaaggccgt aacaacatcg ttcgtttatc tattcgtcca gatgcggttg ttttatacgg
    14341 aaattcgcca gaaatcggga aagtagaaga aagcttgagt tatacagcaa gcagtggcga
    14401 tccattagat atttctttca atccggatta tatgaaagca gcgttgcgtg catttggaga
    14461 tatgagcatc aaagtgaaat ttatctctgc tattcgtcca tttacattgg aaccaacgga
    14521 agatggcgtt caatttattc agctgattac tcctgtacgt acaa
//
LOCUS       DAZUMK010000063        14393 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000063 DAZUMK010000000
VERSION     DAZUMK010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.064"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            147..1886
                     /gene="yfcC"
                     /locus_tag="ACG808_001767"
     CDS             147..1886
                     /gene="yfcC"
                     /locus_tag="ACG808_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFS5047393.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2043..3227
                     /gene="iadA"
                     /locus_tag="ACG808_001768"
     CDS             2043..3227
                     /gene="iadA"
                     /locus_tag="ACG808_001768"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFS5047394.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3338..3541
                     /locus_tag="ACG808_001769"
     CDS             3338..3541
                     /locus_tag="ACG808_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFS5047395.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3642..4304)
                     /locus_tag="ACG808_001770"
     CDS             complement(3642..4304)
                     /locus_tag="ACG808_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5047396.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4317..5819)
                     /locus_tag="ACG808_001771"
     CDS             complement(4317..5819)
                     /locus_tag="ACG808_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5047397.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6367..8046
                     /locus_tag="ACG808_001772"
     CDS             6367..8046
                     /locus_tag="ACG808_001772"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFS5047398.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8173..8652
                     /locus_tag="ACG808_001773"
     CDS             8173..8652
                     /locus_tag="ACG808_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFS5047399.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8871..9551
                     /locus_tag="ACG808_001774"
     CDS             8871..9551
                     /locus_tag="ACG808_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFS5047400.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9559..10890
                     /locus_tag="ACG808_001775"
     CDS             9559..10890
                     /locus_tag="ACG808_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFS5047401.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10905..11867
                     /locus_tag="ACG808_001776"
     CDS             10905..11867
                     /locus_tag="ACG808_001776"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFS5047402.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKINVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11978..12316
                     /locus_tag="ACG808_001777"
     CDS             11978..12316
                     /locus_tag="ACG808_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFS5047403.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12395..13102
                     /locus_tag="ACG808_001778"
     CDS             12395..13102
                     /locus_tag="ACG808_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFS5047404.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(13205..>14393)
                     /locus_tag="ACG808_001779"
     CDS             complement(13205..>14393)
                     /locus_tag="ACG808_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5047405.1"
                     /translation="MLLINYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLR
                     ILSVKNCCTLEGNELSIRLYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISK
                     QEKNIYLLLTLFILRNKQQAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEET
                     ETERAYFNYFFYCFFPNQISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHL
                     ENKIPICFYFLVLAVVFFELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIF
                     SKNGERNQISQYLSQLIQHLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAV
                     KITAYSEHADLIITDQHEKISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKT
                     KRM"
BASE COUNT         4586 a         2495 c         3109 g         4203 t
ORIGIN      
        1 tcatacatag gagaaaaaaa caaaatagaa aaaagtgatt ttctaataaa ttattcatgt
       61 ggaaatgtca tttttactgt ataattcatt tgattatcca ttttttattc atcaaaatta
      121 ctgaaaaaag aaaggaagaa ataaatttga agaaaacaaa ttcttcagga aaaccgaaac
      181 gcgacttcac taaattatcg acaccccata cgtatgtcat tatttttggt gtagtcatct
      241 ttgcatggat actgactttt gtcgttccag caggaaagtt cagcacgcaa gatattgaat
      301 acaaagatgc aaatggcgaa acaagtactc gtactgtttt acgtcaagat tccttccgtt
      361 atgcttacga gctggataag tcgtatgtgt ttgatcaatt agaagaactg caagatcatc
      421 cagcagaacg tgaaaaactg gatgttccgg aaaaaggatt ggaaaaagtc attgctgacg
      481 gagaaaaaaa tcttacgcag gaaaaactag atgaaatctc cttgacagat gatgtgcttt
      541 acgatcagta cggcgaaaat atctatgata cttctaaaaa attgcataaa acagccaaaa
      601 tatggggaac agatgatttt ggcggctttg gtttcttaaa ttttgttttt gaaggactag
      661 tatctggaga taagtatgga tctgctgtag ggatcgctgc attgatcctt gttgttggag
      721 gtgcttttgg tattattatg cgtaccggag cgatcgacgc aggtatctat gcgtttatca
      781 gtaaaactaa gggactggag cgtttagctt taccgctatt attctttgct ttttcattcg
      841 gtggcgctac attcgggatg gcagaagagg taattccatt ctcaatggtg atggttccat
      901 ttgtcattgc gttaggatat gactcgatcg tagcagtaac cgtgacttat gtcgcatctc
      961 aagtcggaaa tgcgacctct tggatgagcc cgtttagcgt agcggtggct caagggattg
     1021 ctggaatccc cgtgttgtct ggtgcgacat tccgattgat tatgtgggta gttgttactg
     1081 cattagccgc tggttatatg atgatatatg cagaaaaaat ccgtaaaaaa ccagaaagtt
     1141 cgttgactta caaatcagat gattatttcc gttctcatat caaaaagaca tctgatgaaa
     1201 ataaaaaatt catgcttggg cataaattga tcttattaga gatgttagtt gtccttgtct
     1261 ggatcgtttg gggcgtgact caaaaaggct attacattcc agaaatcgct tctcaattct
     1321 ttgtgatggg attagttgct ggaatcattg ctgttctttt caaattagat ggaatgaaag
     1381 tcaatgacat cgcttcttct ttccaatctg gtgcagctga tttagctggt acagcaatcg
     1441 tagtaggtat ggccaaaggg attcttcttg tcttgggagg atctgatgca tctgttcctt
     1501 cagctttgaa tacgattttg catgggatcg ggacggcgtt gaccggtgtt ccagctgtca
     1561 ttggtgcttg ggcgatgtac atcttccaaa gtttgttcaa cttggttgtt acatcaaatt
     1621 caggacaagc agctttgact atgccaatca tggctccact ggcagattta gtaggtgtat
     1681 ccagacaagt agcagttctt gcttatcagc ttggttcagg ttttatggac gcttttacac
     1741 ccgtatcagc cagcttgatt ggtgtgctag gtgttgcacg gatcgaatgg gcaaaatggg
     1801 caagattcca aatcaagatg caaggattcc tgtttgtttt aggaactatt ttcatcatga
     1861 ttgccattgc aataggtctg cagtagtact ggagacctgt aaataaaaaa gtaatccaaa
     1921 cagtagataa ggactccaag aaccgtcatt ttacggtaaa atcaaaggag taaagatcct
     1981 atcgtttgga ttatttttta agatcctatc atcagttata cttaaagaaa gaggaaaaat
     2041 gtatgaaaat cattcgacaa attgaagtgt ttgcaccaga ttacttagga aaaatggatg
     2101 tactgattgc cggtgggaag attttggctg tagaagaaca gctagaaggc ggctacgaag
     2161 gagtggaagt agaagaactt tcaggtgagg gaaaagtact aaccccgggt ttcattgact
     2221 gtcatttcca tattttaggt ggaggaggag aaggcggtta tcaaaatcga acgccagaag
     2281 tcacattgag ccaattgact acagctggtg taacgacagt tgtcggttgt ttgggaacag
     2341 acggagaagg tagagatatg actgcgttga tcagcaaagc gaaaggtctg gaagcagaag
     2401 gaatctccac ttatgtatac gaaggttcgt accgattgcc ggtaaaaaca gtaacagatt
     2461 ctatcatcaa agactttttg acaattgata aaattatcgg aatcggagaa atcgcagtat
     2521 ccgatcaccg ttcttctcag ccaagttttg aagagttcgc tcgtgcagta gcagatgctc
     2581 gtgtaggagg catgttatcg ggaaaagctg ggatcatcaa tgtccactta ggcggaggaa
     2641 aaagaaagct agagctattg acacgaatcg tagaagaaac agaaattccg atcacacaat
     2701 ttttgcctac tcatgcaaat cgcacaccag aattatttga agcttgcgtg gcatttgcca
     2761 aacgtggtgg cacgattgac ttcactgcta gcgaagatcc tgatttttgg gagaagacag
     2821 atggtgaagt tcgcttcagc aaagcactga aacgattgat cgaggaagat gtttctttag
     2881 ataatttcac gatgacctca gatggacaag gaagcttgcc ttattttgat gaaaacaatc
     2941 atttcctagg gttaggagta ggaagtgcca aggcattgct agtagggatc aaagaagctg
     3001 tgcaaaaaga aagtatccct ttggaaatcg ccttacgagc tataaccagt aatccagcac
     3061 gtatattgaa attggataaa aaaggaaaaa tcgaaattgg tgctgatgca gatctgtgta
     3121 tcttggataa agaaacgctt gatattgata cagttatcgc taaaggagaa ataatggtcc
     3181 aagaaaaaga agtcaaagta tgggggactt tcgaaaaaag tttttaatca acagctctaa
     3241 ctatcttttt tagaaaacga gcgttctgtt tgtctgtatc tgttgttgat gttaccctga
     3301 aggtagatca ataaacaaac taagaggagg aagcacaatg gcagatttaa aaggacgttc
     3361 taaagatatg aaagataaag tagtcggtga aggaaaagaa gcatacggca aagcaacaga
     3421 cgacaagagc aaagaaacag aagggaaagc acaatcacta ggtgctgaca tcaaagaaaa
     3481 agctcgaaat ttaggcgacg aagtcaaaga caagttttct cgaaaagacg aaaaaaaata
     3541 aaaaccactt attactaatt gaatcaagca aataaaaaat ccaaaccata tctgcgagac
     3601 agatatggtt cggatttttt atttgatcat tatcctgttt tttaaacttc gattttttga
     3661 aattttttat tttttagttc aaagacttca tcacagcttt ttgctacttc aatctcatga
     3721 gtcacgatga taatacagcg attttgttca tgggcgattt cttggaaaag tttgacgata
     3781 tcttgtgaag ttgtttcatc tagatttcca gtaggctcat ccgctacgat tagttgatgt
     3841 tcacagcaga tagcacgaac gatagcgact cgctgctgct gacctcccga aagtttggtc
     3901 acctgttttt ttgctaatat ttcatcaatt cctacacgag ccagtcgatc taaagcaact
     3961 tgtcgataat tctcttgctt ggtcttagtg atttccattg ctgctatgac gttgtctaga
     4021 gccgataaat aaggaagcaa gttgtaagct tggaaaataa tcgagacatc atttttacga
     4081 taacttctta acccgatttt gttcagtgaa cgatcttgat agtatacctt tccttctttt
     4141 ggtgcatcta gtccagaaat aagcgaaaga aaagtcgttt ttccagaacc acttgctcct
     4201 aaaatagcgt acatttttcc agcttcgaaa ttcagttcta catctttata aagcgcatct
     4261 tgcggatctt gataccaaaa tcccaattgt tctgtccgta acatcaaatt cctcctttag
     4321 tttaatagga tttttcttgg gtttaatctt aatatcccta tagaagctaa aaagacagaa
     4381 aacagactga ttccaaatgc tactccggct aatagtccca attgtgcagg tttgacattg
     4441 atttccaatt cgctgacttg atcagaaaca gcaaatggat tattcgtggt tccttgttga
     4501 ccatcgcgtt gaccgccttg ttcttgtctg ttacctggca tttgtccacc gccaggagct
     4561 tgctggtttt gtgtcgtggt ttctgtctgt tggtcaagta gctgctgtcc cacggcattc
     4621 gcgacgacgt ttccgctaag cgaagcaact cctaatgcaa gaatcataca aatagccact
     4681 tcggtaaaca attggagaat gattttactt ctggattctc ctaaagatag caagacgcct
     4741 aactcatgcc gacgctcccg aatggtaatc ataatgatca gagtaagtat gataataccg
     4801 gctacagcca ctagtaaaac aacattttta gaaaaacttg ccacgttgtt taaaggttcc
     4861 aacattgact gatacatgga atcatttgat tgtaagctaa atgtatctgt gtcgatcagc
     4921 ttctcagctt ctttgagaaa ctcatccatt ttatctggat cagatagagt gtaaatagca
     4981 gaatctatcg tgttatcgtc agatgtccca tttagttcat ttgcaaaagt gtaagaagtg
     5041 taaagtgtat tcgacggatt catgaagttg aatttcatac ccatactgcc gcttgtttcg
     5101 ctggattcat aaattccttt gatcgtcatt tcataagtgg tatcttcatc ttcagtactc
     5161 gtgattgtga aagtatctcc gactgccagg tcatttgttt ctgctagagt agaatcaatg
     5221 actacattgt ttgtgccttt gtcgctgtca tctattcctt caccatccgt gatttttgct
     5281 gttccatcag aaaaattact attttgtgca gttgctgaca caccactgat ttgaaagtct
     5341 gcttgagtca tttgcggatt cccgcccatt cctccagcag gttcaccgcc tgcatcagta
     5401 gtttctgaac tgtcttcact tgaatcagaa ctagtgatgg cagttattcc atcttttgcc
     5461 atagctgagg cggaactttc aaaggaataa ctctttacat tatctaattc agctattttt
     5521 tccgcgtccg ttaaggaaac tggtgtaaga gaaaaactgc ctggatctgg acgttcttca
     5581 gtggtagaac cgcttgaaga agtttgttcc tgttttttaa atgaagcttc tcgattggca
     5641 cttaaagtca ctgttgctcc aacttctttt tgtgcttgat tcgctgcttc ttctgcagcg
     5701 cttcgaatcg tgaggccagc tagcacaaaa ataagaatgg ctgtgaacac ggcaaacaat
     5761 aacaatgaac gtccccattt tacttttgta cttttccatg cacgtttcat aaaattcata
     5821 ttttgcatcc ctcctagtag atgcatttga taatagacaa cctatcttaa agctagctta
     5881 aatcttatgc tgtttgcaca ccatcaacat gaggttctgt atatataaaa agtcactata
     5941 gttattgcaa aaaatgtggt aaaatgaaaa aagtatgttt caaaccgacg aagaagaaat
     6001 gaaagaggaa tagcaaaaga aatcctagat gctacagagg gatttttacg tgcatagttg
     6061 ttctaacaat tgctgatact agcgatttag tttatttgtt gtataataat ggggaacagc
     6121 tgaatactta tataagtatt ctggctatag cgtttcgcaa aagatccatt ttgttaatga
     6181 agtatatcca aaataaaatg cccatccaac tgtcagaacg cggatgtgct ggtaattttc
     6241 ctgctgattc tccataagtt gagaaatagt gattttgcat atgcttcatt ggaatggctc
     6301 atatatttag ataaagaaga aaatcgacat ctttaaactt ggatgtcata agaagaggtg
     6361 aagtttgtga gtaccattaa aattatccca ttaggcggcg ttcgtgaaaa tggaaaaaac
     6421 atgtatgtcg cagaagtggg cgaggatatt tttattttag attgtgggct aaaatatcct
     6481 gaaaatgagt tgttggggat cgacgtcgta atccctgatt ttacgtattt agaagaaaac
     6541 attgatcgtg tagctggtgt ctttttaaca catggacatg cagatgcgat cggggcactt
     6601 ccttacttgt tgtcaaaaat ttctgtacca gtattcggga cagagttgac gatcgagtta
     6661 gcaaaattga atgtcagtcg taatgaagca gccaaaggat ttaaggattt ccatgtggta
     6721 gatgagcata cagaaataga ctttggtgaa acagtagtga gctttttccg taccactcat
     6781 acgataccag attcagtggg gatcaatctg aaaacaaaag aaggtagtat cgtttatacg
     6841 ggagatttta aatttgacca aacggctatc ccaatgtatc aaacagatta tgcacgattg
     6901 gcggaaatcg gcaaagaagg agtcttagcc ttactaagtg aatcggcaaa tgcagaaaat
     6961 cctcagcctg tagcttctga agcccaaata gcagatgaag tatttgatac gatcaaatat
     7021 tgggaagggc gtatcatcgt cgcttgtgta gcaagtaatt tgcaacgagt gcaacaagta
     7081 ttggatgcag cagaaaaagc acaacgaaaa gttgtcttga ctggtcaaga tttcgaacga
     7141 attattcgca cagcgatgcg tttagaaaaa cttcaattac caagtgaaga tctgcttgtt
     7201 acgttgaaag acatgaaaaa ctatgatcca gaagaactga tcattttaga aactggccga
     7261 atgggtgaac ccatcaagtc tctgcaaaaa atggcaaacg gtacacaccg taatgtcaag
     7321 attgaagaag gagatttggt ttatatcact actacgccaa gcatcgcgat ggaaaccatc
     7381 gtggcgaaaa cggaagatat catttatcga gctggtggaa cagtgaagtt gatctctgag
     7441 aatatgcgcg tttctggcca tgccaatcct aatgacttgc aattaatgat caatttgatg
     7501 aaaccaacat attttgtgcc agttcaagga gagtatcgtg aattagctgc tcatgcagat
     7561 ttggctcatg cagtagggat gccatacaaa aatatctata ttactggacg cggagacgtg
     7621 ctggaatata agaacaagcg tatgagtgtt gcaggaacta caagtgcaga aaatgtaatg
     7681 attgatggat taggcatagg tgatatcgga aacattgttt tacgtgatcg aaaaatcttg
     7741 tcagaagacg ggattttcgt agcggttgtc actatcagtc ggcgagaaaa gaaaatcatt
     7801 tcaaaacctc aaattacttc acggggattc gtttacgtaa aagctagccg tgatctgatg
     7861 aaagaaagca gcaatatgat tgaaacaatc gttgagaaac atttagaaag caatgatttt
     7921 gagtggagca aattaaagca agacatccga gatcaattag gccgttactt atttgaacaa
     7981 acaaaacgcc gcccagtgat cttgccagtc attatggaag ctactcaacg tcgaaataaa
     8041 aaataaacaa aaagagtaag atgaacctct ctatagagtg ctcgtcttgc tctttttttg
     8101 cgaaaaacaa ataatttttg aatacagcat ccctcgcttt ataataaacg atagaataaa
     8161 gggagtgaat caatgatgag tcaaacaaaa gtcgaacgtt atttagaaga acaaaagatc
     8221 agctatcaac cacttgattt tagtcatgtg gtagaagaat ctttcacaga agaactacaa
     8281 aaaagaggga ttgatccagc tttgatatgt aaaactctcg tagtgaaagg aaacaaaact
     8341 ggggtgatca tcgcattgat tcctttgaac gatcacttag attacaaaaa aacgagacaa
     8401 gtgacgaaag atcgtaaagt tggttttcct ggaatggatt ttgtattaat gcatacagga
     8461 tatccccacg gagcaaacac ccctattgga ataaaactcg cacatcctga ttattgtttt
     8521 ttagcagaca gttctttaaa agaaaaagaa gaagtaattg tgtcaagcgg tgaaatcggg
     8581 agaagccttc ggataaaagt agccgattta gaaaagatcg tccatcctat ttacgctgat
     8641 ttaattagtt aagggacgag aagagaagct aagcagagca gagacctcaa acttgagatt
     8701 atatctgctt ggtacttggt gcatcctgta ttatctgtta caattgcttt gtacatactg
     8761 tactatcaat ccaatgaaaa tatatttagc agctatattt tttcattgat gtgcaagtga
     8821 tgaaatcgtt tttaataaaa aagatacaga gaaaacgcga ggtgtaaaat atgaaaatta
     8881 cgtatcatgg acattcctgt ttaacaattg aaatggaaaa tggccaacgc gtgatgattg
     8941 acccgtttat tacaggaaat ccattgacag acttaactgc agaagaagta aagacagatt
     9001 ggttgctgat tactcatggt catagtgacc acataggaga tatgattcct attgcaaaag
     9061 aaaatcaagc cacaattatc agtattgttg agattgctga ttatgcgaac agcagaggag
     9121 tagattcatt cggtatgaat atcggtggaa aacatgcgtt tccattcggc gtggtaaaat
     9181 tcgtacatgc acagcatagt tcaagctatg aagtagacgg tatcgttcag tatatgggcg
     9241 agcctagcgg aattattatt caagcagaag gaaaaacgat ttatcatgca ggggatactg
     9301 cttatttcag tgatctagga ttgcttgcag aagaattcga tattgatgtc gctttcttgc
     9361 cgattggcga taactatacg atggggccag aagatgcagc aaaggctgcg caaacaattc
     9421 gtgccaagaa agtagttcca atccattaca atactttccc tgtcattgaa caagatcccg
     9481 aatcttttgt agagttactg gaagatagtg aaggaaaaat aatgagcgtc tcggaaacgc
     9541 tggaagtgta ggcagaaaat ggcgacaaaa catgatttga ttttagaata tattgaaagt
     9601 ttacctgttg gcaatcgaat ttctgtaaga agcatcgcaa aagaattgaa tgtcagtgaa
     9661 ggaacagcgt accgtgcaat caaagatgca gaaaatgtcg gactagtttc aacaattcaa
     9721 cgagtaggga ccatccgtat cgaacgcaaa ttaaaaaaac atattgaacg tttgacattt
     9781 ggcgaggtcg tcaggatcat tgaaggggac gttttaggcg gggcggcagg attagacaag
     9841 gtcttgaata aattcgtcat cggcgcgatg actaaacatg ccatggaacg ctatattacg
     9901 ccaggatcac taatgatcgt cggcaaccgg gcggaagtcc aaaaattagc attggaagat
     9961 ggcgccgcag tattgatcac aggaggattc aatacgactg ctgagattgc aaagctagct
    10021 gatgatcttg aaatgccgat cttacgcacc tcttatgata catttacagt cgcaaccatg
    10081 atcaaccgag cgttaagcga ccaattgatc aaaaaagata tcatgcttgt cagtgatatc
    10141 tatatgccag ctgaaaagac tcattatttg cagcagacag atacgatttc tgattatcag
    10201 cgtttgtcag aagaaacgca gcattcgcgt tttccagtcg tcaaccgcca tcatcgtttg
    10261 atggggattg ttacggcaaa agatgtttta gggaaatcgc caaaccagat catcgatcgg
    10321 gtgatgacga aagaaccaat cagtgtaaag aaaacaatga gtattgcctc tgttagccat
    10381 caaatgatct gggatgggct agaagtcatg ccggtcgttt ccgatgactt gacattggaa
    10441 ggcttgatta cccggcaaga tgtaatgaaa gcgatgcagc ttgtccagcg acaaccgcag
    10501 attgcagata cgatttcgga tcagatatct ggaaatatcc atacgattga tactgaccgt
    10561 gaaggaaatc gtttggaaaa ccctaagttc aaattcgtag tagcaccaca aatggtcaat
    10621 ggcgttggaa ccatttcttt tggcgtactt agcgaaatca tttctgatgt cgcacaaaaa
    10681 acaatggtga tgaaccaaaa acgaaatatc ttgattgaac aagtgaacct acattatttg
    10741 cggctgatcc aattggaaag cgaattagac attcgtccac gtgtattgga aatcggcaga
    10801 cgttccgcta aactggatat cgaagtgttt attgaaaaca ccatcgtttc caaagcaatc
    10861 gttgtttgtc aagtaatgga acgttcttag aaagcaggag tgaaatgaca gtacagcaag
    10921 aaatagtaga tacaataaaa aaatacgata aaatcattat ccacaggcat ttgcgccctg
    10981 atcctgatgc attaggatca caagtaggat tagccgaact attacgggaa agtttcccag
    11041 aaaaagaaat acgtcaagta ggtgaaacga ttgaagggct tcgcttttta gcagaaatgc
    11101 aagaagttga agatgtcttc taccaaggag cgctagtcat cgtgactgat acagctaatt
    11161 cacccagaat cagtgatcaa agatatcagt tggcagaaaa actgatcaaa atcgaccatc
    11221 atcccaatga tgacccttat ggagatcttg tgtgggtaaa tacaaaagca agtagctgta
    11281 gtgagatgat tgcagaattt gctttgatgt ttcctgaatc tttaaagata aacgttaagg
    11341 ctgcacggtt actttatgca ggaatcgtcg gagatacagg gcgctttttg tatccctcca
    11401 cgacgtcccg tacattagag attgcttcga tattgagaaa ttatccattt gatgcaagtg
    11461 ctttgaacag agagattgaa caaatgccga tgaaagtcgc taaactttcc ggttatcttt
    11521 atcaaaatat ccaggtggat gaaaatggtg caggaagaat aatccttccg caagaaattc
    11581 ttgataaata tggaatcgat gattcagaga cagcttcgat cgtttcactt cctggagtga
    11641 ttgacgaagt gctggcttgg gggatttttg ttcagcagcc ttcagggtat taccgcgtac
    11701 gtttacgttc taaggggccg atcatcaatg aattggctaa acgccatcat ggaggaggcc
    11761 atccattagc aagtggtgcg aatgcaaaag atttagcaga agtagaggaa atctataaag
    11821 agatccaaga tatatgccaa tcgtataaga gcagaaaaac agaatgatac ttcgttcgaa
    11881 aacgtgcctt ttgtagtaaa aatcacaaat gtatcttttc aagatcttct ttttccgtta
    11941 caatagtgct cactaaagat tttaaaggag aaagaagatg acattaccaa attgcccaga
    12001 atgtggctca gagtacacat atgaagatcg aggcttgttg atttgcccag aatgcgggaa
    12061 cgaatggaac aaagaagaac aagaaactgt catagaagac ggattagtgg tattagatgc
    12121 aaatggaaac cgtctgttag agggcgatag cgtgacagtg attaaagact tgaaagtaaa
    12181 aggtgcgtca ggtgccatta aacaaggaac aaaagtcaag aacatccgtt tagtagaagg
    12241 cgatcataat atcgattgca aggtagaagg atttggaccg atgaaactga aatcggaatt
    12301 tgtgaaaaag aattaaagaa tattcaaggt cttttcacat tcttttccta ctactaatga
    12361 taaaataaag taaacgaaaa ggagtgaaac gaaaatgtat ggttatggaa tgtatggttt
    12421 tttagatccg acatttcttt tggtcattct tggattagtt atttccggaa ttgcgtcagc
    12481 gtacgtcaat agtactttcc gaaaatacga tcaagtgaga agcaaaaaca atgtgactgg
    12541 tacacaagca gcacaatata tattacaaag tcaacagata aataatgtcg gtgttcaaca
    12601 aattgccgga gatctcacag ataactacaa ttcaggtaat aagatgttga gtttgtctca
    12661 agcaactgca cagtctactt cagttgctgc gataggtgta gcagctcatg aatgcggaca
    12721 tgcggtacaa gatgcttcgg gttatgtccc attgaagatt cgtgcagcac ttgttccagc
    12781 agctaacttt ggttcgatga tttcgttccc cttgattatg gtaggtgtat tgcttagctg
    12841 gaactctacg ttaatcaata tcgggatttt cgctttttct ttagcattac ttttccaatt
    12901 ggtgacatta ccagtcgaat ttaacgcgtc tcggcgagca atccaaattt tgagtgaagg
    12961 cggactactg acggaagaag aagtaccgat ggcaagacac gtattatttg cagcagcact
    13021 gacttatgtt gcagcagcgc tttcgacatt cctgcagctg ttgcgcttga tcttattgtt
    13081 cggcggcaac cgaagagatt aaaataaaga agttgtgaaa tcagcgcttt gatctgagct
    13141 aataccgaga gttagctgat tgaataccat ttatttggaa taagaggatg tgacaagaat
    13201 tttgtcacat cctctttgtt ttgttctcat aacctttttt catatacagt tcatgaaccg
    13261 cttttattaa cttgccaaat aacactgtat tgtgcgttgt atccagatag aaagtctccg
    13321 tttcatctga aattttctca tgctgatctg taatgatcaa atcagcatgt tctgaatatg
    13381 cggtaatttt aacagcatct ttgcgaaata actgctgcaa atggctttga agatgataat
    13441 ttcctgtaaa ctgtttggtt atctgcaaat ggatgagtaa ctgctctttc gtttctgttt
    13501 gtagtaagtg ttggatcaat tgactaaggt attgactgat ttggtttctt tccccatttt
    13561 ttgaaaaaat tgtatcgatt ttttctctta atttcttttc ctgtataaaa gaactagaat
    13621 gattggacgt ggcttctgga aagcaaagtg tagaaaaact actgctggat tcacccaaca
    13681 gttcgaaaaa aacaactgcc aacacaagaa aataaaaaca aattggtatt ttattttcta
    13741 aatggttgag aggaaactgt tgagttaagt aagtcatcaa ttgaccgact tgtcgacaaa
    13801 taaggttttt gcttgtggaa aataaaaaac caatctcctg tttttgtcga aaagagattt
    13861 gatttggaaa aaaacaatag aaaaaataat taaagtaggc tctctctgtt tctgtctctt
    13921 cacaagggcg actcactgaa aaagaagtaa agtattctga ataatcatgt gtatgctgca
    13981 ataagtccat caattcagtg atttcagaag aagtttttgg aaaatatgcc tgttgtttgt
    14041 ttcgtaatat gaataaagtt aacagcaagt agatattttt ttcttgtttt gaaatttttt
    14101 ctatttgctc atctgtactg ttcattttta gtgcatttaa ttcaagtgag gaaaatggcc
    14161 aatcgttacc tccaaatgta tctgaaagaa aaaagtaaag atagagacgg atagataact
    14221 catttccttc aagagtacag caatttttta cagataggat ccgtaaatgg aattttttta
    14281 aggaatgatt gagctgtttt gtcagttttt cgagatagga gggcgatata taaagttgtt
    14341 caagtaaata tttttttggt acagcagttt gtggataatt gattagtaac atc
//
LOCUS       DAZUMK010000064        14335 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000064 DAZUMK010000000
VERSION     DAZUMK010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.065"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            287..2758
                     /locus_tag="ACG808_001780"
     CDS             287..2758
                     /locus_tag="ACG808_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS5047406.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2861..4597)
                     /locus_tag="ACG808_001781"
     CDS             complement(2861..4597)
                     /locus_tag="ACG808_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5047407.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4594..5298)
                     /locus_tag="ACG808_001782"
     CDS             complement(4594..5298)
                     /locus_tag="ACG808_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5047408.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5429..5932
                     /locus_tag="ACG808_001783"
     CDS             5429..5932
                     /locus_tag="ACG808_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFS5047409.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5892..6230
                     /locus_tag="ACG808_001784"
     CDS             5892..6230
                     /locus_tag="ACG808_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFS5047410.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6251..7669
                     /gene="ffh"
                     /locus_tag="ACG808_001785"
     CDS             6251..7669
                     /gene="ffh"
                     /locus_tag="ACG808_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFS5047411.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7781..8359
                     /locus_tag="ACG808_001786"
     CDS             7781..8359
                     /locus_tag="ACG808_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFS5047412.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8363..8884
                     /locus_tag="ACG808_001787"
     CDS             8363..8884
                     /locus_tag="ACG808_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFS5047413.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8963..9517)
                     /locus_tag="ACG808_001788"
     CDS             complement(8963..9517)
                     /locus_tag="ACG808_001788"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFS5047414.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9770..10045
                     /gene="rpsP"
                     /locus_tag="ACG808_001789"
     CDS             9770..10045
                     /gene="rpsP"
                     /locus_tag="ACG808_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFS5047415.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10057..10308
                     /locus_tag="ACG808_001790"
     CDS             10057..10308
                     /locus_tag="ACG808_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFS5047416.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10433..11224
                     /locus_tag="ACG808_001791"
     CDS             10433..11224
                     /locus_tag="ACG808_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047417.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11394..11915
                     /gene="rimM"
                     /locus_tag="ACG808_001792"
     CDS             11394..11915
                     /gene="rimM"
                     /locus_tag="ACG808_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFS5047418.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11905..12666
                     /gene="trmD"
                     /locus_tag="ACG808_001793"
     CDS             11905..12666
                     /gene="trmD"
                     /locus_tag="ACG808_001793"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFS5047419.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12802..13149
                     /gene="rplS"
                     /locus_tag="ACG808_001794"
     CDS             12802..13149
                     /gene="rplS"
                     /locus_tag="ACG808_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFS5047420.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(13185..13370)
                     /locus_tag="ACG808_001795"
     CDS             complement(13185..13370)
                     /locus_tag="ACG808_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047421.1"
                     /translation="MLEYSGFFIKAVTLKKDIIIVLFFLLLLIGRFILIHYSPLPIIK
                     EKTGSGCSNYMLRKKNP"
     gene            13407..13700
                     /locus_tag="ACG808_001796"
     CDS             13407..13700
                     /locus_tag="ACG808_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFS5047422.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(13788..13934)
                     /locus_tag="ACG808_001797"
                     /pseudo
     CDS             complement(13788..13934)
                     /locus_tag="ACG808_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
BASE COUNT         4792 a         2585 c         2923 g         4035 t
ORIGIN      
        1 gtatgattat ccattgagag actcctttgg taggatgacg ttttggtcga ctaaatttta
       61 ccaaaagtga gtctcttttt gagttgaaac gaagaaaaag tgttagtaga aaatcgcctt
      121 cgattttcca ccaacacaaa atattataga accctttttt tttgctatat gacaggaatg
      181 aaaggtgttt gcaagcttaa tgtgaggaac actttccata tctgttctat actggatagg
      241 agaagtttgg aaatatccag aaaacgaaat caatcgaggt agccacatga agaataaatg
      301 gataaatcga agtaaaaaaa agaaagtaac acaaagatca tcagataaag tcatccgtct
      361 ttttccatcg tataaagaag gattaacggc agatcaagtc aaggaacgaa tagaaaaagg
      421 tgcagcaaat aattcagtcg atcccacatt caaaacaaat cagcaaatcg tattggaaaa
      481 catatttacg tatttcaatt tgatctttct tatcttagcc attttgcttt gtttagtaga
      541 atcctataag aacttgactt ttttaccagt tatcatagcc aatacaggaa tcgccatttt
      601 tcaggaaatt cgatccaaaa agatcttgga cgaactaaat gttttaaata cagcaaaagt
      661 caatgtcata cgagacggga tagaacaaac aatagatata gaagaccttg taatagatga
      721 cattgttatt ttagagactg gatatcaaat tccagcagat gcagaggtcg tggaagggaa
      781 actgcaagtt aatgaggctt tattgacagg cgaagcagat gaaataacca aagaaatagg
      841 tgatccatta atgtccggca gtttcattgt ttccggaaaa gcttacgccc gattagacaa
      901 agttggagca gattcgtata tttcccagct gacagtcaaa gccaagacaa tgggagaagg
      961 agaacagtcg gagatgattg cttctcttaa tcaattgatc aaatggattg ggatcatcat
     1021 tattcctatc ggtatcctgc ttttttctca aagttacttt tttaatggaa acacgatcaa
     1081 agaaagtgtc gttgcaatgg aagcagcatt gatcggtatg atacctgaag gactgtactt
     1141 gctgacgacg attgcattag cattaagtgc gactaaacta gcaaagcaaa gagttctttt
     1201 acacaatatg aaaagcattg agacattggc aagggtaaat gtcctttgtg tagataaaac
     1261 aggaacgatc acagaaaata aaatgtctgt tcaaaaaatg atcgtctcta aaaaacaaga
     1321 aataatcagt cagtcagatc aattggaagc atgcattgct gactatgcaa aagcgatggc
     1381 aggagacaat gcaacaatgg aagctgttaa agcatatttt acaaaaacca ctgatcaatc
     1441 ctatcaccat gttattcctt tttcttctgt ccaaaaattc agcagtgtag cgttggcaga
     1501 caaggtgtat gttttaggag cgcccgaaat ggttttgcgc gatcaattgc cggaatatgc
     1561 ggaagagttt gtttctttcg ctgaaaaagg ctatcgcgtg ttggtctttg gtgtgtacga
     1621 tgggattctt gaagaaccag tattgacaga agcagtcaca ccattaggct atattctgat
     1681 tgctaatcct atcagaaaag aagcaagagc cacatttgat tacttcaaaa aacagcaagt
     1741 agccatcaaa gtgatttctg gagataatcc gctcaccgta tccaatgtag ctgtccaagc
     1801 agggatttca catgctgatc agtacgttga tgcatctaaa ttatcagaag aagaatatga
     1861 agcagctgtc gaaaaatata cagtttttgg acgcgtcaag ccagaacaaa agaaaatctt
     1921 cgttcaattg ataaagcgta acaatacagt agctatgaca ggtgatggcg tgaatgatat
     1981 tctagccatg aaagaagcag actgcagtat agcgatggct tccggaaatg aagccgccat
     2041 gcaggcctca caagtggtct tacttgattc tgacttttca cgcatgccag aagttgttgc
     2101 agaaggacgg cgcgttgtga acaatattga gcgatcagcc agcttgtttt tagtgaagaa
     2161 tattttctcc tttttgctct cactgttttc agtgatcttt gctctgacct atcctttaga
     2221 accatcacaa atcacattga ttagtttgtt tactatcgga cttccatctt tcttgcttgc
     2281 tctagaagaa aacaaaaagc gaatcagagg gaaattcatc atgaatgtga tggaaaaagc
     2341 tgtgccaggt ggactaacag atatgatcgt agtcggtgcg ttggtgatct gcggggtgac
     2401 tttgaatcta aataaaacag atgtttctac agcatctacc atgcttttga ttgcagtagg
     2461 gttcctcgtt ctctataaaa tctgttcacc gctgaataaa ttcagaagtc agatcattct
     2521 tttctgtgct agcggtaact ttttttcggt catctttttg cataagctgt tttcaatcac
     2581 cggtatctca gctgtatcac ttttgctgtt gagcattcta tttttctcgg cagattcgat
     2641 ttttaggcat ttgaccacat tggtagaata tttgtttgaa cgtaaaaatg aaaaagagca
     2701 aaagccgttt agtcgcgtag ctaaaaaatt tctatcgata tttaaacgag aaaattgacg
     2761 aaaagtatct caaaaaagag taaaaaagaa caaccactcg tgtatctttc gaagcaggat
     2821 gagtcatcca actttaaaac gcacgagcag ttgctctttt ttacattttc ggaatggtaa
     2881 cggtgaaagt cgaacctgtt ccaagatgac tgtctacagt aatcgtacca ttaaggagtt
     2941 gcacgtagtc ttttacaatc gctaacccta atccggtccc gcctgaatgt cggcttcgcg
     3001 ccttgtctac gcggtaaaaa cgctcaaaga tccgttcctg atcctcaatg tcgataccta
     3061 taccaaagtc ttggacagaa aacgatagat catctgttgc ctgatagcgg ataataatct
     3121 cgctgtcgct ttttgaatat tggacagcat tttcaatcag atttttgata attggataaa
     3181 ggatatctgt tttagtagta aatggttcgt tctttccaat caatcgaatc gtcaaccgtt
     3241 tcttttcgat gattggctga taattttgaa cgatttgctg gaaataaggt tctgtataaa
     3301 ttgtctggat ctcgtaagaa ttctcgccat cttttgacag ctggatgatt tcccgaatca
     3361 gtttatctaa ccggatcgca tctttttgca tgatctcaag aaaagaagtc agagtttgcg
     3421 ggtcatcttt tgcaccatcc agtaaagtct cggtaaaacc aatcagtgaa gtcacaggag
     3481 tttttaattc gtgagagacg ttgccaacga aatccttttg tagcttttct aaacgtcgca
     3541 ctttcgttaa gtcatacgcc acccctagaa tctgtccggt accttctgaa ttagtaaagt
     3601 agcgcaaact caagtctaat gtctgccgac cttctgtaag ggtgatctcc tgatggatca
     3661 gcggatgctc aggagtcact tgatgaatca gctgaatgag ttttggctct tgaatgactt
     3721 ctgtgtaccg ctgttcaggt tgatagctta ccacgtttaa atgtagctgc atctttggat
     3781 taagcagtag taaacgagaa tcagcatcat caatcaggaa tacgccaatc atcagttcgt
     3841 tcagtaacgt atataattgc tcttctgtgg tagtacacgc ccgatacatc ttactcattt
     3901 gttcgctcaa cgcattaatc gtctgataca attcttccca ttgatcgggt gtttgcatga
     3961 ttacttccgc ctgattagga ttttgcacca ttttttttag tacgggcagc acggttttca
     4021 atggctggtt tttttgatag atcagataat agatcattcc tgcaagaaca aggaagaaag
     4081 acagaaagaa gaaaaagacc caacggcgaa aactctcagt tctcggtaaa aaaacgcttg
     4141 tcggttctgc gatacgtaaa atggcttcta actggccact ttttttgact ggtaatgcga
     4201 catataaaag atcctcattc aaagtagtac ttttgcgcaa agcgctacct aggttcccac
     4261 cattcaatac ggctttgatt tcgggacggg agtttcgctg ttcattcaat gtttcattgt
     4321 ttgtgtcaaa taagatcttt ccagtagcat ccattaaagt aatccgttcg ttcgactgat
     4381 ggacgaattc ttccaaagca gttttgtttt gcgctgcttg gaggttttcg acatctaatt
     4441 gccggatcag cagttgtgct ttttttgtca ggtagctttc ttgctgttcc acgatctgct
     4501 gttgaaaata atgggaaatc aattgccagc caccaaaaaa caaactaact aaaagaataa
     4561 gaaagaagag acgttcataa atttttttcc atttcatcgt ttgggttcct gaaacttata
     4621 gccaaatcct ctaatagtca ccaagtactt tgggtgtttt ggatctcgct cgattttttc
     4681 tcgcaaatga ctaacgtgta catctacgat tcggctttgt cctgcgaagt caaaattcca
     4741 aatgcggtct agcaatgtgt ctcggtcgat cacacggtct tttcgtttca tgaaataaac
     4801 aagtaattca aattctttcg gtgttagttc aatcagatgg tcgtctacgg tcacttgata
     4861 attggtcaaa tcagcagtga tttgtccaat cgatagatat tcaggctcag cttcatcttt
     4921 tgattgctta cgaggttcaa ttcgtctgaa tattgctttc atacgggcta atacttcgcg
     4981 aggactaaat ggtttggtca aatagtcgtc agcacctatt tctaggccaa tgatccgatc
     5041 cacttggtca tctttagccg tcagcatcaa aatcggtgtt tcattttttt cttgacgcag
     5101 ccgctgagtg atctccatgc catccatcgt tggaagcatc acatctaaaa tgatgaaatc
     5161 aaatgattgt tctaatgcta attctaatcc ttcgcctcca tctgtggcac tggttacctt
     5221 gtagccttct ttttccaaat tgaacgtcaa gagcgttaca atagaaggtt catcatcaac
     5281 aactagtatt tttttcattc aaatctcctt taaataggtt tcattctgtt tatattttag
     5341 catagaatca gtacaaaggg ctattttaca aaagaaataa atggtataat aggaaggcac
     5401 tttgctgaat agaaaggaag agcagtcaat gattggaatc agtgcatgtt taggcggtct
     5461 ggcctgtcgc tatgatggac agaaaaaaga agtaccagaa ttgatggaac tagttgaaac
     5521 tggagaagct gtcatggtct gtccagaagt gatcggcgga ttaccaattc ctagagatcc
     5581 cgctgaaatc attggtggaa atggattcga tgtgtggaaa gatcaagcaa aagtatggac
     5641 gacaagcggc gaagatgtaa cggaagcgta caaaaatggc gcgatcaaag cctatcaaaa
     5701 attgcaggaa aaacacattg atctagtcat catgaaggaa aaaagccctt catgcggaac
     5761 ccattcgatt tatgacggaa gtttttccgg aataaaaaaa aatggtgccg gagtggctac
     5821 tgcttatttt attcagcaaa aaatgactgt gctctctgat gaagaatggc tgaaaactag
     5881 aggtgagcag aatggaaatc gaaaagacca atcgaatgaa cgcgctcttt gaattttatt
     5941 cgaccttatt gacagaaaaa caaatgaact acatggaatt atattatgca gatgattttt
     6001 cattaggtga gatcgcggaa gaatacgaag taagccgaca agcagtatat gacaatatca
     6061 agcggacaag caagatcttg gaggattatg aaaaaaaact ccatcttttt tctaattata
     6121 ttgttcgaga acaggtattg gaaaatatga ctgcatatat tacagataag tacccggaag
     6181 ataaaaaact acttgaatat gtacagcaaa ttcaagcaat cgaagaataa ataaaaggaa
     6241 tgaagaaaat atggcatttg aaagcttaac tgaacgtctc cagcaggcga tgggaaaaat
     6301 aagaaaaaaa ggaaaagtat cagaagctga cgtaaaagaa atgatgcggg aaatccgcct
     6361 tgccttacta gaagccgatg ttaacttgca agtagtcaaa gattttacta agcgcgtacg
     6421 cgaacgtgcg gtaggggcag aagtgctgga aagtttatca cctgcacaac aaatcgtaaa
     6481 aatcgttgat gaagaattga cgatcacact tggttctgaa acagcagagt taaataaatc
     6541 accaaaaata ccaactgtta tcatgatggc tggtcttcaa ggggcaggta aaacgacctt
     6601 tactggtaaa ttagccaatt atttaaagaa aaatgaaaat gctcgtccgc tcctaatcgc
     6661 aggggacgta tatcgaccag ctgcgatcga ccaattgaaa gtattaggtc aacagctaga
     6721 cgttcctgta tttgatatgg gaactgaagt cagcccggta gagatcgtcc gtcaagggat
     6781 ggagttagca aaagaaaaga aaaacgacta tgtattgatc gatacggcag gacgtctgca
     6841 tatcgatgaa acattgatgg atgagttgaa acaaatcaaa gaattgacgc aaccaaatga
     6901 gattttgctt gttgtcgatg ccatgactgg tcaagatgcc gtcaacgtag ctgatagctt
     6961 caaccagcaa ttaggtatta caggggtcgt catcactaaa cttgatgggg atactcgagg
     7021 cggggcagca ctatcgatcc gctcagtgac aggcgcacca atcaaattca tcggttctgg
     7081 tgaaaaattg actgacttag aagtattcca cccagatcgt atggctagcc gtatccttgg
     7141 tatgggggat atgctgacat tgattgaaaa agcgcagcaa gactacgacg agaaaaaagc
     7201 ggaagaactt gcacagaaaa tgcgggaaaa ttcattcgat ttcaatgact ttattgaaca
     7261 gcttgatcaa gtgatgggta tgggcccact ggaagatcta atcaaaatga tcccaggaat
     7321 gaatcaagtc cctggaatcg aaaacgtcaa ggtagatcca aaagatgttg aacgcaaaaa
     7381 agcgatggtt tattctatga cgccagcaga aagagcaaat cctgatctgt tgaacccaag
     7441 ccgtcgtcgc cgaattgctg ccggttctgg aaacagtgtt gttgaagtga atcgaatgat
     7501 caaacaattc aaggaatcaa gaaaaatgat gcagcaaatg tccaaagggg atatgaacat
     7561 cccaggtatg gaccaaatgt ttggttcagg cgtcaaagga aaactaggca aaatggcgat
     7621 gaatcgaatg atcaagaaaa ataagaagaa aaagaaaaaa cggaaataag atgaaaaaaa
     7681 cagcttcggt acagactgaa aatgtctact gccggagctt ttctatcagc aatttttatt
     7741 ctttactaat acaattactc taaacgaaga ggtggcacag atgagatgtc cgtggagtga
     7801 acaaacagaa agtatgcggc aatatcatga tactgtctgg ggtgttcctg aatatgatga
     7861 ccaaaagctg ttccgaaaat tgatgcttga tattaatcaa gccggattga gctggcagac
     7921 gattttaaat aaaagtgaag cattcgatga agcatatgat cgattcgata tcaaaaaagt
     7981 cgcaaattat ggagaaacaa aaatcagtga actaatggaa aacaaaggaa ttattcgtaa
     8041 ccgaagaaaa atcgaagcag ccgtccaaaa cgccaaagca gtcttgaaaa ttcaagaaga
     8101 aatcggcagt ttttcagagt atctttggtc gttttctaat ggaaaaatcg ttgattcttc
     8161 ttatcattca caagaagaag tcccaacaac aagtcctctt tcagatgaat tagcaaaaga
     8221 tctaaagaaa agaggattta aatttatcgg aagcacaaca atctatgctt ttttagaagc
     8281 tgtcgggatc atcaatgatc accttgacag ttgtttccgc aaagaagagg tcgtacgtat
     8341 cggcagaaag ggcgaataaa gcatgcgaaa aatcatattt tatggtgcta taagtttaga
     8401 tggctatttg gcaacgaaaa cggatgatct tcaatggctt tttgacactc caacaggtaa
     8461 gcaaactact tatgaagctt tttatgaaac aatcgatacg accatcatgg gcagaaaaac
     8521 ttatcaggaa gcaaaaaaat atttggagac cgaagcaatt tatccagaga aaaagaattt
     8581 cgttttttcc acccacgcac aattacaatt gccagatgct gaggtcgtaa acgaagatcc
     8641 tgttgctttt ctaaaaaatc tacaaatgac agaaggaaaa gatatctggg ttgtgggtgg
     8701 cggtcgattg atcaaaccgc tattggaaca tcaaatgatt gatgaatggt atatccagat
     8761 tacccctgta ttattagggg acgggattcc tttattccaa aaaggaacat atgaagcgcg
     8821 ttttgaaatg ctagacacga cacgattcgg tgaatttatc gaattgcatt atgtacgaaa
     8881 gtagaagtaa ataaaataaa aaaggcaact atatgcagaa atgtcctgta catagttgcc
     8941 ttttttatta ccgttatgct ttttactctg ttaatttgtt agaaaaatct agatacgcat
     9001 ctaaaatcgt ttggaagaaa cttaatgttc cttcaacgat attaccattt tcatcaatta
     9061 gattcgcaac gtttccgata tatgcttccg gttgctgcaa ggttggcaca ttcaaaaaga
     9121 caagtgattg gcgtagatgg tggtttgcgc caaagccgcc gatcccacct ggtgatgcag
     9181 taacgactaa acctggttta tgatcccaaa cactgtgtcc ataaggtctt gagcctacat
     9241 ctaaagcatt tttcaatact gctggcacag aacggttgta ttcaggagta acgaaaacga
     9301 caccgtctag tcccctcact tcctcacgaa aacgagtcca ttcagcagga gcctgttctg
     9361 gagtttcgag atcctcattg taaaatggca gatcatcgat ttttacaaat acactttcgt
     9421 acccttcagg aagcaatttc gcaaaagctt ctgctacttt tttgttgtaa gaatcttttc
     9481 ttaaactacc aataaaaaat ccaatttttt ttgtcatacg atttttcctc cttcaaacta
     9541 tctataataa tcttacaaaa gataagtaac tggagcaaat aaaaacactt actgacaaaa
     9601 gctacataga agatagatta cttattttta acaaaaaaga agtctaaaat cttctatttt
     9661 ctaaaaaaaa tcgaatttaa tcaagctgta aagaaaaaaa gctttacagg gtaaaaaaaa
     9721 actgttacac tacatcttgt gaataaaaca atggaggtgg aaataaataa tggcagttaa
     9781 aatccgttta aaacgtatgg gttctaaaaa gagtccgttt taccgtatcg tcgtagctga
     9841 ttcacgttct cctcgtgatg gacgtttcat cgaaactgta ggtacttaca atcctttgaa
     9901 agaccctgca gaagtagttt taaaagaaga cttagttctt gactggttat caaaaggtgc
     9961 acaaccttct gacactgtac gtaacatcct ttcaaaagaa ggcgttatgc aaaaacacca
    10021 cgaagctaaa ttctcaaaga aataaggtga ccggatatga aagatttaag cgacttagtc
    10081 ttaacaatcg ttcgtccgct agtcacttat cctgatcaag tcaagttaga cgtcgtagag
    10141 tcgaaagact tttacgagta caatctaaca gtggctcctg aagatattgg ccgtatcatt
    10201 ggaaaacaag ggcgtgttgc taaagcaatc cgcacaatcg tttacggtgt tcggatcaat
    10261 gcaccgaaaa aagtgcgttt gaatatcatc gatgataagg aaaagtaaat aaacgtgctg
    10321 tactcgaatt ttcgagtgca gcttttttgt tttttttatt aattgaagaa atgattattt
    10381 ggcaaaacaa gcaagaaagc gtatccttat aggaggaggg agtgaacagc caatgagaaa
    10441 gaaaatgtta tttattccat ttgctctatt gttaagcgct tgtagttcgg ttactcaaga
    10501 atcagacaat acacatctat caagcagtat atctgaaact gttaccagtc cagaaaaaag
    10561 tagtaccaaa acggcttcca ccactgaaca aacaatggct acctcaaata atgagaaaaa
    10621 gactgcttta gaccagttga aagaacaaca gccaaatgtt cccatgcctc tagatgtacc
    10681 agtctcttca ggctatctca atatcgcagc gacacataca aagcaaggtt attctatttt
    10741 atattacaga acagatcgtc ccctcgggtt gaatgctgac gaattgaatc aagaaacgcc
    10801 tattgcaacc tatctatatc aatatgggtt tgcttctagt caagaaacaa tccaagtttt
    10861 gcagccattt gagatcgaca caaatggtca gcaagtggac ttaggttctc gaatcacagg
    10921 gtatcagcaa ggtgcagcag gttcaagttt tcttgaatgg caagaaggaa actggcgtat
    10981 tcgaatccgt gggaataata tagaaggcca agatccctta ctattagcta aagaaatcgt
    11041 agcttatttg gaagaaaaca gtctaccagc acctgaacaa tttggtaaaa ttactgttga
    11101 tatgggtgat acgacgaatc gagccgtaga agtaagctgg caagaaccga aaaatgccta
    11161 tacgatcact catcaagatc ctatgtctgc gttgaagatg gcagtttcta tgaagaggtt
    11221 gtgaaatcag cgcgctgatc tgagcccatc atttaactcc aagtcacagg aagtaatcaa
    11281 aaatagcacg ccgcattttt gcataatttt ggcttaatac cttaaaagaa aactagaatt
    11341 gcctcttcac acatgctaaa ataaaacaga caaaaaagaa atggggatat aagttgactg
    11401 aatacttgaa tgtaggaaaa atcgtaaaca cacaaggtat caaaggcgaa gtccgtgtta
    11461 tctcaacaac ggatttccca gaagaacgtt acaaaaaagg aacggtgcta acactattcc
    11521 aagacggaaa agcacctgtg gaattaacag tcaaaagtca tcgcaaacat aaaaattttg
    11581 acttattgag ttttgaagga catccttcta tcaatgatgt ggaaaaatat cgggatggca
    11641 tattaaaagt gtcaaaagac aactctgtag atctagctga aaatgaattc tattaccatg
    11701 agatcatcgg tctgcatgta ttagaagatg gaactgagtt aggtaaagta aaagaaatct
    11761 tgtcaccagg tgccaatgat gtttgggtcg ttcaacgtcc gaaaaaagct gatatattgc
    11821 ttccatatat cgcttctgtc gtcaaagaag tcgatttaga agctggagtg gtccgtgtag
    11881 aaattccaga aggactgatt gacgatgaag attgatgtat tgactttatt tccaagaatg
    11941 tttgaaggtc ctatggggga atccatcatc ggtaaggctg tagataaagg attattagat
    12001 atcaatatct ctaacttccg cgattattca gataacaaac atcagacagt agatgattat
    12061 ccatacggcg gtggagcagg tatgctgctc aaagtccagc ccatctatga taacatccaa
    12121 gccattgaaa aagctgcacc ggatgtcaaa aaaagagtaa ttcttcttga cccagctggt
    12181 aaacgattcg atcagaaaat ggcagaagaa ttttcacaag aagaacactt agtattcatt
    12241 tgtggtcatt atgagggata tgatgaacgt atccgcacat tagtgacaga tgaagtttcc
    12301 ttaggagatt atgtccttac tgggggagaa ttaggtgcga tggtcatgat cgatgcaaca
    12361 gttcggcttt tgccagatgt gttagggaat cagacttcag ctcaaacgga ttcttattca
    12421 accggtttat tggaacatcc ccaatatact cgcccggcag aatataaagg gatgaaagta
    12481 ccagaagtat tgacaaatgg aaatcataaa ttgattgaag aatggcaatt aaaagaatca
    12541 ctaagaagga cctatcaacg tcggcctgat cttttggaaa cacttgaatg gacacctcaa
    12601 atgacgaaat ttttagaaga aatcaagaaa gaagaacaag aaaaaacagc agatgaagcc
    12661 aagtgacttt ttctaaaatc ttctttacag taggttgata atatgttagt attttatggt
    12721 gagtgcaaaa gcacttaact attacgatat tccgctgtga gaaaagctca taagaatatt
    12781 tggaataagg agaagaaaac catgaatcca ttaatcgaag aattaacaaa agaacaatta
    12841 cgttctgaca tcccagcgtt ccgccctggt gacactgtgc gtgttcacgc aaaagttgtt
    12901 gaaggtactc gtgaacgtat ccagttattt gaaggtgttg ttatcaaacg ccgcggtgct
    12961 ggaatcagcg aaacttatac agtacgtaaa gtttctaacg gagtgggcgt tgaacgtaca
    13021 ttcccattgc atacaccacg tgttgcaaaa atcgaagtag ttcgctacgg taaagtacgt
    13081 cgtgcaaaat tgtactacct acgtgcatta cacggaaaag ctgctcgtat caaagaaatc
    13141 cgtcgttaat cattgttatc agactgagaa agaacccttg gtattcaagg gttctttttt
    13201 cttagcatgt aatttgaaca ccctgatcca gttttttctt taattattgg taggggagaa
    13261 taatggatta gaataaatct acctataagt agtagtaaaa aaaagagcac tatgataata
    13321 tctttcttaa gagtaaccgc tttaataaaa aatccagaat attctaacat aaatggagta
    13381 ttcttagaat caaaaggaga aagaagatgg tttatactta tacggagcaa gaaaagatga
    13441 aagttttaga cacattcatg aatgatggaa aagtaaaaca agtaccaagt tcaacaaaaa
    13501 agaaatacat cctattgaaa gaagtcttga aacgctttga ccatggagtg acttatacag
    13561 aaacagaggt taattcgata ctgctgaacg tattttctag tggcgattat gtcgaacaaa
    13621 gacgttatct aatcacattt ggctttttca aaagaagttc agacggtcgg gcatatcaga
    13681 tgatgggaat tgaaaattag aagttttaat ttatactcat tgaaaaaagt tcaagtatgc
    13741 gatattggtt ctacaccgca tacttgaact ttttttgtca tctgtatcta taatacaaag
    13801 ccaagaacgt tattcaaaat atggatagct atgataaaag ctaatgagcg attcgcagtt
    13861 aaataagcaa tcgtcagtaa aatcgttcca ggagcgtaca tgaaaaaatc aattggatgc
    13921 gttacatgaa taaagataat tctcttttat ttgtacgaat aaataccaaa acgtacctcc
    13981 taaaattaga ttttggacag atgcccttta aaaagataac aagatttcgt tcttgaaaca
    14041 atatatatgc ttttattcca atgaggttgt gaaaacagta ttttgtcctg agcgttagaa
    14101 aattgaaaat ggggaatagc aactcgtact ttaccatttt ttaagtgaat acaagatcag
    14161 ctaattgaac accgtttatt cgtgatgagg gacacaacac tgcagtcgac acatttctct
    14221 tttatgttca aagataacaa atatttaaaa caaagaaaaa tagtagataa tcccgtccta
    14281 actcttttat tcttccctca tagcttccaa atttaagcaa tacttcaatt aatca
//
LOCUS       DAZUMK010000065        13736 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000065 DAZUMK010000000
VERSION     DAZUMK010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.067"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(63..1268)
                     /gene="mobT"
                     /locus_tag="ACG808_001798"
     CDS             complement(63..1268)
                     /gene="mobT"
                     /locus_tag="ACG808_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFS5047423.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            complement(1291..1444)
                     /locus_tag="ACG808_001799"
                     /pseudo
     CDS             complement(1291..1444)
                     /locus_tag="ACG808_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(1447..2832)
                     /locus_tag="ACG808_001800"
     CDS             complement(1447..2832)
                     /locus_tag="ACG808_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFS5047424.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            complement(2861..3247)
                     /locus_tag="ACG808_001801"
     CDS             complement(2861..3247)
                     /locus_tag="ACG808_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS5047425.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            complement(3263..3577)
                     /locus_tag="ACG808_001802"
     CDS             complement(3263..3577)
                     /locus_tag="ACG808_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS5047426.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            complement(3930..4409)
                     /gene="rlmH"
                     /locus_tag="ACG808_001803"
     CDS             complement(3930..4409)
                     /gene="rlmH"
                     /locus_tag="ACG808_001803"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFS5047427.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            4837..6126
                     /locus_tag="ACG808_001804"
     CDS             4837..6126
                     /locus_tag="ACG808_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFS5047428.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(6165..6770)
                     /gene="ytpR"
                     /locus_tag="ACG808_001805"
     CDS             complement(6165..6770)
                     /gene="ytpR"
                     /locus_tag="ACG808_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFS5047429.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(6966..7430)
                     /locus_tag="ACG808_001806"
     CDS             complement(6966..7430)
                     /locus_tag="ACG808_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS5047430.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(7452..7772)
                     /locus_tag="ACG808_001807"
     CDS             complement(7452..7772)
                     /locus_tag="ACG808_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFS5047431.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            complement(7915..8994)
                     /gene="pepA"
                     /locus_tag="ACG808_001808"
     CDS             complement(7915..8994)
                     /gene="pepA"
                     /locus_tag="ACG808_001808"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFS5047432.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            9194..9544
                     /locus_tag="ACG808_001809"
     CDS             9194..9544
                     /locus_tag="ACG808_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFS5047433.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            9729..10682
                     /locus_tag="ACG808_001810"
     CDS             9729..10682
                     /locus_tag="ACG808_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS5047434.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            10695..12482
                     /locus_tag="ACG808_001811"
     CDS             10695..12482
                     /locus_tag="ACG808_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6020 family protein"
                     /protein_id="HFS5047435.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            12469..13671
                     /locus_tag="ACG808_001812"
     CDS             12469..13671
                     /locus_tag="ACG808_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047436.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
BASE COUNT         3938 a         2689 c         2520 g         4589 t
ORIGIN      
        1 aggttttgtc caaaattcat aaaaatgttc tcctctctat attcaatgaa tttgtatttg
       61 agttattttt ttgttgttat cacgtcctgt tcttttactg actgttgctt caaaatctgc
      121 ttgtgtcggt ctgtcagttt cgcatggtcg agaatgtctt ttacaacctg cgtctggttg
      181 atttcatcaa gtttaatcgc aacctttaag gtcggggcaa cttgatgaga tagccagttc
      241 agcgtccttt ggaaggagta aggctctggt tttgtggtta gttttaatcg ttcacgattg
      301 ttcccaataa accaagccca ttcttcattc agtttccaat cagaacgagg tttggaatcg
      361 tctttatcta caaaacggat ataccgattg ataattttaa aggcggtatg ctctggattg
      421 tcatagacga gtaaatcacg gactgcataa taggcacgct catttttcaa tcgaatctca
      481 aaacggtttt ttacttctgc gtcttcaatg ggaatatcat ttttcttgta ctgctcgtag
      541 tccttttcat agatacagaa ataaacttca ctttgtaatg aaccgatata gagggtgttt
      601 cccatacatt ccttttcctc tttgcgtacc agttcgccac tgcgatagct tttaaaactg
      661 cggaagacgg agatacattc ttcctgttgg cacttttcag tgagtacagg gatatttaaa
      721 atccctgtct tatcgttaat ggcaaggtca aggcgtttca tcacaccgcc agccaccaaa
      781 acgtccataa agaactcata ccagcttctt tgttgtgcca gaagatagct ttcaaattgt
      841 ctgcacccac gacctttcaa ttccaccaga actcctttgt ccagttcatg ggagcaaagg
      901 acgaatatgt cgcctaaagc ataatgctct gaataagaat agaaaccata gtcctcatga
      961 agaaaatagg acagtttcag ttgtaagatg ttttcgacca cctgctgtac gtctgttgtc
     1021 ggaaagcgaa ttcttacata atcaaacagc atttcaaggg gagcgtcggg attgaagcgt
     1081 tccagagctt cccaaaggga ctgctgtaaa tcctctgatg gcttgacttt tcctgtttca
     1141 atatcgctta gatactgcct tgtaatacca gtcgcaacag ctaaacggtt ttgagatagt
     1201 ccataagcca agcgtttttc ttttaaatgc tgtaaccaag tttgttcatt cagtaaaaat
     1261 ccctccaatc aaaaaggcgt atgtcaactt ttaaagccca tttgacatac gctgaaattt
     1321 tgtaaatccc ttgtaaccaa aggattttct aatgtttttt tgactgtttc ctgtcgattt
     1381 gtacccccct gttagatacc gaggggttaa gtgctggcgt ggctattgcc acaccagcca
     1441 gcaagatcag tccacacctg cgacttccgc ttcgcacgtc gcctgcgtgg actgtctgct
     1501 gttggataac tttttaattt cctccaagaa atcatatcct tttggtacaa ggggagtata
     1561 aaactctgat atgacacttg ttcctacatc aacatagcca cgacctttga ttcgctttaa
     1621 gaagaaatcc ttttgtacgt cactgccaaa catcatgcca tagcccattt cagacatacg
     1681 acctaaagcc actctgaaat taaactgatc acggattccg tcgcctaaat attttgcgtc
     1741 tggacgttga caagccagta ttagaaagaa gccagcttga cgacctaaca tgacaatctg
     1801 tttcagctta ttcataactg cggtgttttc ttttgttccc agcatttcca tgaaagcgac
     1861 gtattcatca aagattaaga agtgtgccgg gagacctaag taagcataat ttttgccagt
     1921 cttatagttc ttcatctgct tcatttcctc actacgtttc atcatttctt catagaatgt
     1981 ttcaatgcaa gaaagcaagt cttcttttct atagtagaca tttgccatca cagaacctaa
     2041 gtccgcaagg tcagcatttt tcgggtcaag aatatacagt tttgaatctg tatgaagcaa
     2101 ggcttcaatc agtgtcagta taaagtaagt tttaccgcca cctgtaccac cagcaatcaa
     2161 catatgaggg agcttatcat attcccacca tacgtttttc attaagcgaa gtttaccatc
     2221 tttagcttct acttcatcaa tagaaatacg actggctatg gtgtcataga gcaaagtata
     2281 ttccacatag gaatccttta actctttatc cgtcagctca cagtacaagc cactctctaa
     2341 tttcttttcc aagtgtaaga gttggtcttg atattttccc agcgtgattt ccacccgtat
     2401 ctgtatcaag ccatttttaa gtcgataata cattttaggg aagtaggtta tcttttcctt
     2461 tgtacgacca gcactatctt taaagaaacc ctctgttttg acctgttcag attcatacca
     2521 cttgttttca agtatcatct ttgccagttt ttgacggtgg taaagttgtt taaccgtatc
     2581 atagcgaacc cgtttgaata caaacgctac cagcaagcag ataagaattg cgacactgaa
     2641 actgataatt aaatagggaa tgtcaatctt atctgcttgt gataggttaa aatcctgcca
     2701 gttgatctgc tggattgtct tcacatgaaa cagtccgaca accagcagga aaacaggcag
     2761 gagtgacgct atcgtaaaat gaaagactaa atctttacca gatgggcgaa tccttttacc
     2821 acgctgtttc atgcgaaaaa gtctcctttc tacctagcga ctatttgtct tgtgtcggtt
     2881 ctttctttgc ttgtggttga gctttgaatg aactagaatc ctttgtcagc acaatatcgt
     2941 ctgccttgat ataccagtca acatctgctc cttgataggt ggcagtagca acggtgtccg
     3001 caatgggatt gataagttcc acccgtgcgt tataatcaaa ctctttcaaa ggcacgctgg
     3061 caggaatact tacttgaatc atgcgtcctt gtcctttgga ttttaagtca taggtacgtt
     3121 ccttgatttc atctgaaacc gacccgtctt cattttggat tctcacttca cgacgtagag
     3181 cagagaattt caattctcca aaagtcgtgt ctttatctaa tacaatgcca tttgctaatc
     3241 tcatcatttt tcctctcttt ctttattctt ttatcatgtc gtcagcatgt aaaaggtaat
     3301 ttgtaaaacc acgagtgccg attttgtagc cctctgcggt aatacgtgga ttgactaact
     3361 tcacacgttc ctcaaagccg aaatgttttt cgccagcttc agcaggaagc accaccacaa
     3421 tatcatctgc tctttgaaca tcagaataga gattatagct tcttgataag acagttagcc
     3481 gtccgttgat tcttcgctga acgactttat cctcgccagc aaattctaaa ttgccgaatg
     3541 ttttttccat gttgggaatc acaaatttaa gttccatatt tttacctatc ctttcttttt
     3601 tattggctga atgaatgttt gatggtctta aagagtgggg aacgaccttt tgattcttga
     3661 ttttttgttt tcataagttc acttcctttc aaaatcgggt aaaaaaatag acacctcatt
     3721 ttttgaagtg tctacctatt aaatattcaa attttattgg aagtatcttt atatcttcac
     3781 ttttcaagga taaatcgtcg tatcaaagct cattcataag tagtaaatta gtagtaaatt
     3841 gagtggtttt gaccttgata aagtgtgata agtccagttt ttatgcggat aactagattt
     3901 ttatgctatt tttctcttaa aaaatatcat tatttgtggt atggagcccc cgcgttgatc
     3961 cggaaggcac ggtagatttg ctcgacgagg atcaggcgca tcaattgatg tggatacgtc
     4021 attttgccaa aagagatctg agcattgctt cttttcatga cggcttcgct gagtcctaaa
     4081 gaaccgccga taacaaaaac aaggtggctt tttccttgga tacctaattg gtcgatttgt
     4141 ttagcaaaag attcgcttgt gggatttttc ccttcaattg ctaagccata gacgtattct
     4201 tgttctttga ttttcgctaa gatccgttct ccttcttttt cttttacttg tctcatttgc
     4261 gcttcgctta aattctccgg ggctttttcg tcaggaactt cgatcaattc gattttcgca
     4321 taggcattca gccgtttgac atactcattg ataccttgga tcaagtattt ttcttttaat
     4381 tttcctactg aaataatttt gatattcatg ttttctctcc tttgatcttc acgctcactt
     4441 tgtttattat aatcaatttt ttcgtctata tgtttatttt gactattttt gttaaaaaga
     4501 tttccccaac agttatgcac aacatccaca ggtttatcca cagaaaacag cgttgtttta
     4561 ccttatttca tcacattcat taggcaaaca tacgttttat aagaatttat ccactttata
     4621 cacaggctgt ggatatgttt tatcccttat ttttccaatc ctatccgcgt agttttacac
     4681 aatatctgtg gaaaaagaaa atcaaatatt ttcatctgaa aacaatgatt tttcctaaag
     4741 tttttcataa aaagttcaaa gtctcaaagt agactaaagt caacaaataa gactatacta
     4801 acagtagata aatgaatgta attggaggag gaacacatgg atagaaaaaa tgtgacacca
     4861 aagatgaaaa aaaataaaaa taatagcctc tggcgtaagt tgggtctggg gcttgttgga
     4921 gggatcgttg gaggactact gacagctggc attttttatg cagcgatggg cactggcaac
     4981 aatagttcta ctgctacaag cggcaatcaa aattctgccg gtgaaacagt tgtggaaaat
     5041 gtcaaagtca atgtagattc tgatatcacc aaagcggtag ataaagttca agacgctgtc
     5101 gtttctgtta tcaacttaca aagtcaaaac caatcaagcg gttttggcgg attattcggg
     5161 cagcaagaag aaagtagcag ctcagatgat agtaatttgg aagcttatag tgaaggaagc
     5221 ggcgtaatct acaaaaaaga cgggaatact gcttatgtag tcacaaacaa tcacgtagta
     5281 gatggacagc aaggtttgga agtcatgatg aaagacggta cgaaggtaaa agctgaatta
     5341 gttggaacag atgcctattc agatttagcg gtgttgaaga tcaattcaga caaagtcgaa
     5401 acggttgcct cttttgggga ctcaagtgct ttgaaggttg gtgaacctgc gattgcgatt
     5461 ggttccccat taggatctga atatgcgaat tctgtcactt caggaatcat ttcttccttg
     5521 aaccgacaag taacaagtac gaatgaatcg aacgaaacag tcaatatcaa tgcgatccaa
     5581 acagatgcag cgatcaaccc gggaaattct ggtggtcctt tagtgaatat cgaaggacaa
     5641 gttatcggta tcaactcaag taagatcgca agtacttctg aatcgacttc taatgtaagc
     5701 gtagaaggaa tgggatttgc catcccaagt aacgatgtag tgaatatcat caaccaactg
     5761 gaaaaagacg gaaaagtgac acgtccagct ctaggtatca caatggtcga cttatctgcc
     5821 gtttctactc agcaacaaga acaaatcttg aagattcctt catctgtaac taacggtgtc
     5881 atcgtgacaa atgtccaaac agctacaccg gcagaaaaag ctgggttgaa acaatacgac
     5941 gtcattacta agatcgatga caaagaagtt tcttctggtg tagaacttca atctgctttg
     6001 tataagaaaa aagtgggaga ttctgtcaaa atcacttact atgaaggctc gaaagaaaaa
     6061 acaactactg ttgaattgac gatcgatcaa tcggcattaa aacaaaatca aaatagtgaa
     6121 aattaataaa aaagaggttg tgacaaagac ttgtcacaac ctctttattt tggaaatact
     6181 tctccgacta cggcatcctc aggcaatact aagattcctt ttttttctgg agcatttggc
     6241 aaatgcaatt cacgagcaga acaaatcatt ccatagctgg ctacccctcg caaagttcct
     6301 ggccagatca tcattccatc tggcatcatg gctcctggtt ttgcaacgac aactttttgt
     6361 ccagcttcga tatttggtgc accgcaaaca atttgcaata ctgtcccatt gtccacttct
     6421 gtctcagtaa tcgataaatg gtcagagtca ggatgcggga cgcatgtttt tacatacccg
     6481 acaacgatct tcggttcatg atcagcttca agctgttctt cgaatcctgc ttctttaatc
     6541 aatcgattca atttcgctaa ttcttcatct gttaagctta cttggccgtt cccttcaatt
     6601 gttagatgat cggagatatg gaaaaagttc caagcaacaa cttgaccttg ttcatctttg
     6661 atccgagcaa cattccattt acgttcgaca gtgttttctg ttcctttatc gtcggctatg
     6721 ataaccatca aagtgtctcc gacgtgtgct ttgttataag caaaaatcat gttctttcct
     6781 ccattatcct ttgctgagat ttatcttaac aaaaaattgc attcactgca tgagattttt
     6841 ttcatgcttt atttccgaag aagcttcttc tggaacacca tttattcgta acaagggact
     6901 gtgacaagac ttttgtcaca gtccctttac tgtttgatta aaaaggttat acaagttcca
     6961 cttgcttaat tactcggctg ttcttcttcg tcagaaacga tcaagacatc acaaggtgaa
     7021 tgacgtatga cgtagctggc tacacttcct gtcataaagc gttcgactgc atttagtcca
     7081 gactgcccaa ccatgattag atcgattgca tatttttttg gcaatttgac tgccatggat
     7141 tcacgaacag agccgtatgc aataattcct tctatatttg tgtaatcaac agattttcca
     7201 tattctttac attcttcgat catttgcttg gctgtttcag tttgctgatc gactagattt
     7261 tgatcaatag gagcatagcc cataaaatta taaaattgct gatcgattac gtttgctaca
     7321 tatactttcc cattattacg gcgagccact tctaacgctt ttttgaaagc agcaagtgct
     7381 tgttcactac cgtcaatacc tactaaaatg ttcttatatt cttgattcat cttgacccct
     7441 cctaacagat gtcagccttt tattacactt tctaggaacg attcgatttc ctgttttgtt
     7501 ttacgatctt tattcactag acgacctaat tcttttccgt cttggatcac gacaaaactt
     7561 gggataccaa aaatgttcca ttcagcggct acatctatgt acttgtcacg gtctacttca
     7621 ataaattgcc aatcagaaaa gtcattttcg atttcaggca tttgcggttt gataaaacgg
     7681 cagtctccgc accagccggc agtaaagaaa aatacacttt ttccagtttc tacgtagcct
     7741 gctaattctt ctaaactatt tgggataatc attctttttc ctctactttc tgtttatgta
     7801 tttttcaatt aaattataca cctttctttc gaactaacaa acattgatcc tctttttata
     7861 aaaaaaatct gacaaaaggt tgcttttgcc agattttata gtttcactat cgcgttattt
     7921 cccgtatacg atcgtgttca ctgttgattt atctaaacct ttcaatgttt gaatcaacat
     7981 ttctcgtgca gcttcaaaat cgcgaatact gaacatcgtt tgatgcgtat ggatataacg
     8041 tcccactacg ccaatcactg tacttgggat tccttcattt tgtgtatgag cagcacctgc
     8101 atctgtaccg ccttttgaaa caaagtattg ataagggatg ttgtttgttt cagcgatatc
     8161 tagcaagtac tcacgcaaac gaggaagcat gatcaaacct ggatcttgga tacgcatcaa
     8221 tgtaccttct cctaaatgtc cgaatgtgcc atttttcgtc actgtgtcat ctgctgcaga
     8281 gcagtctact gcaaagaaca gatctggctt gaatttattg acagaagctt tagaaccacg
     8341 caagcctact tcttcttgga cattcgcgcc tgcaatcaat gtatgaccta atgtttcatt
     8401 ttgtaacgct tctaaagctt ctaataccat tgtacagcca tagcggttgt cccacgattt
     8461 gctgatgata ttcttcccgt tggctgtttt gatcgtttca gcgtaaggaa cgattgtgtc
     8521 tcctggaaga acaccgtaac tcattgcctc ttctcttgat tcaaacccag catcgaacaa
     8581 aatatctgtt acttctactg atttttgtcc gcttgttcct cgtaataaat gtgggggaac
     8641 ggaagaagaa atacatggat aattcttacc agtagaggtt ttaagtgtaa aacgttgagc
     8701 agaaacaacg taagggttcc atccgcctag aggtacgact ttaaaaagcc cgttttcttg
     8761 gatttgtgtc agcatgaatc cgacttcatc catatgtgct gcgaccatta cacgaggtgc
     8821 atccgcttct tcatggtggc gcagcccaaa aatgccgccc aaaccatcta actgtaactc
     8881 atctaccagc ggttccatat gactttccat ataggcgcga acgtcttgtt caaaaccgct
     8941 tgttccttgt aattctgtta actctttgat tcgttgaaat gttttttctt ccatatgcta
     9001 ttcgtctaaa tttcagacta aactcctctc agacttatat tgatctgcgg ataaaatatg
     9061 attttttctg cagaccgatc gtatttatta tagcttattt tttctggtag cggttattta
     9121 tttcaaagaa aatatgagag tgtggtaaaa taaacacaaa gagtagcaag tgagaagaag
     9181 gaggtatatc cacatgtatg aagaaagcga attaagctat tttaaaggcg ggttagccgt
     9241 tgggatcggt ctaggattat tgagtggggt agcttcaacg atttggtatc atcgcaaaaa
     9301 agtgttatca gcagaccaag tgcttgaaaa tatcaaaaca tcttttttaa aagaaggcgc
     9361 aatcgaaggt gcttggatct cttttgaaaa agaacctatt cggaaattcg ccgttcggtc
     9421 gagtgcttat cggggtgggc tttcccggat agaagatggc gaaactgttt attatgaatt
     9481 tttagcagat gctcatacag gaaccgtatt agacatttca agaaaaaagg gcgcaataga
     9541 ttaatacgtc aaaaaaatga aataacgaat acgcacccac tatctaaaag agaggtatcc
     9601 tatccttctt ttatagtggg tgatttttat aaaaataggg ctatttcctc ttggaatagc
     9661 tagtatttgt agttattata cggtattctt gaaataagat tagttttatt atataaggag
     9721 agtgacaaat ggaatcacgt gaaaaaattg ccgtgctcat tccttgttat aacgaagagg
     9781 cgacaatttc aactgttatt gaagatttta aaagggaatt accagaagca gatatttatg
     9841 tctatgataa taattcgacc gatcgaacat acgaattggc cgttcaaggc ggagcaatcg
     9901 taaagaaaga accacgtcaa ggaaaaggga atgtaatccg tcaaatgttt tttgatattg
     9961 atgccgatta ttatttgatg gtagatggtg atgatactta tcctgcagaa gctgcacatg
    10021 gtttgcttga gaaattacgt tctggcgaag cagatatggt cattggcgat cgtctatcaa
    10081 atggaaccta ttttgatgaa aataagcgtc ctttccatga ttttgggaat aatctggtta
    10141 aaaatacaat tacttactta tataaaacaa aaattcgcga tgtcatgacc ggctatcgag
    10201 gattcaatcg aatttttgtc aaaagcttcc ctattatgag ttctggtttt caaatcgaaa
    10261 cagaattgac gattcatgct ttagataaaa aattcaagtt cgttgagatt ccaatcgatt
    10321 atcgcgatcg tcctgaaggt agcgaatcaa aattgaatac attctctgac ggattcaaag
    10381 tgatcatgat gatcatcaaa atgtgcaaag attataaacc tttgcttttc tttgggatct
    10441 ggaccatctt cttcttttta tttggattgg tcatggggat tcctgtgatt cgtgaatata
    10501 tgctgacaag ctttattaca cgcatccctt ctgcgatttt aagtactgga ttgatgactt
    10561 tagcccttct ttcattagta acagggttga ttttggatac ggtcgtcaca aatgccaaga
    10621 aagaatatga attgaattta taccatgtct accacgaata tcaaaataaa gaaatgaagt
    10681 aggaggcctt ttttatgcat aagaaaaagg agaatctatt tcctactttt tttgcaactt
    10741 tatttgcgtc aggttttgct ttttttactt cttatggtct aggaactttg aatccaacag
    10801 taatcattgg tttagccatg gttttgctac ttgcttttaa gcaatatgga atagagttgt
    10861 tttcaataaa aaacaaagtt gcagctattg cccgattgtt tttttctgca gcttttgctc
    10921 ttacactttc tattggcaga cagatacaat ttcagtatgt ctcagcaaac tatacaaaga
    10981 caacgattgc tgatggaagt attttttact ttcttgttta tttctccatt tttgtgtatt
    11041 cttttttatt tttacaatcg atttatttat caatgccaaa actggaaaca ttttttactg
    11101 ctcaaaacac acaggctttc tcttcaagaa aatatttttt attctttact gtctttttag
    11161 ttctgtgttg gcttccttat ctgttggcat tgtatcctgg agtggttttg cctgattcgt
    11221 taagttcggt cgcccaatct atgggtgatg ttcctataag caatcatcat ccagtcttat
    11281 tcaccttact ggtcaagcta tttgttcatg atttaccaac tgatacgatc aatcacggtg
    11341 tatttttatt ttcgattttt caaacattga taacagcttc agcgataagt tattctcttt
    11401 gctggctgtt aaaaagaaac atccattttt ttccggtact cattagttta tgctattttg
    11461 catttgcacc ggtttttcca atctacgcct tgaacatgca aaaagatact ttgttctcta
    11521 tcgcttgctt tttattttcc ttagcggttt ttgactttat ttttcaacga gaaaagccaa
    11581 cgattctcaa tcagatttac gtaatcatcc ttgctttact gaccacctat ctaagaaata
    11641 atgggattta tgtagtaacc ggcacaggtt tgatttgcgg cttgattatt ttgaaacaaa
    11701 aaaggtatca agcattcttt caagtggtgc tgggttatac agtgattgct ggactactca
    11761 tccacccgtt gatggctcgt tatgctattc ctacagaaac tgctgaacaa ttaggtgttc
    11821 ctttgcagca aatttctaga actattgtta tggatggtga cttgtctaag aaagataaag
    11881 cattcatgaa tcagctactc ccgataaaag attatgaagc gtattctcct agtcttgcag
    11941 actcaatcaa gtggcataaa aattttaaca atgagttttt gaatacacat aaaaaagaat
    12001 ttctatcact atgggcaaga aatttaccaa gtaatataaa aatctataca gatgcttata
    12061 ttttagaaac gttcgggttc tgggtacctg gtacaaaaaa tagttacggc tttttagata
    12121 ctcgtgtaaa cacgaataat tatggtatga agcaagtaga tttgattgaa catcttactg
    12181 gattgtcagg aatgaaaaaa atcatcgatg ctcgtgattt ctttggttcc ggcacattac
    12241 tgtggttagc tatcggcagt gcacttttag caatctttaa gaaaaaatat cggtatttac
    12301 tcctttttct tcccggtatg cttgtttttg ctactattat gattgctact cctgtggctt
    12361 ttagtttacg gtatgtgttt gttttagcat taggtctacc tgtttatatc ctctctcctt
    12421 ttttactaga taaaagagaa aaaatgaaaa cagatatagg agatactcat gaaactattt
    12481 aaaaagatat tttttgcttt tgttctcttg atcgctagct attgcgtact actgacaatt
    12541 acttttttca ttccgcagca ctctattgaa tcgaatgcag aaaaatctct acagatggtt
    12601 gaaaaagaag gaatgtatcc ttctatcaat caaggaaata tgctaggcac tcggttagat
    12661 aattttactg atcatttgat gatacgaaaa acaaaagcag atcctgaatt gaactccttg
    12721 gagaacgcga tgtctatggc ggattatccc cgttattggc atggttatca gcttttttta
    12781 cgacctttac tgctagggat gagtttgggg agtattcgaa tgatttacgc agccattctt
    12841 tttctgctga ttggattgac gagctacttt ttaaccaagt gtagcgacat ctatttaacg
    12901 acagccttgt tgatttcgct tgctatagga aatgctgcaa cctttttctt cagtatgcaa
    12961 ttttcaaata tttggatttt gactttattg tctgtattac tatttttaac aaaaccacaa
    13021 ctctccaaca ccaaccaaca attattcttg tatttctttg tcattggttc tttagctaac
    13081 ttttttgact tgttgacaac accggtcatc tcttggggga tacctgtgat cattatttat
    13141 tatgtaacaa ataagtattt aatggataaa cgcagttcat tgagcaaaca agtaggcagt
    13201 tttgttttta ccggtatctt ttggggatta ggctatggtt tgacatgggt ggctaaatgg
    13261 atcctatcat cgatcatatt gaacaaaaac attgtaaaag atgcaatcaa ccaaatcctc
    13321 tttcgaacgg aaggaaacga caaatacccg ttgcatcgat tagagatgct gaaaagcaat
    13381 attagattga tgtatccaaa agtagcaata ttattgctat tgattacttg tcttgttttt
    13441 ctctattttg cttatcgtaa acgaaatagc tggcgtccgt tccaacttct agttttgttt
    13501 gttttagctg ctgtaacgcc gtatatttgg tacaatattt tagctaatca ctcacagatt
    13561 cattattggt ttacgtatcg tacgcaaatc atcacaagtt ttgctatact tagtgctttc
    13621 gcctttttga tccctcctga gaaatcaaaa gacgaactaa attactttta agaataccga
    13681 tgtactaatt catttttaaa cataaaaact ccagctgtca gagatagccg gagttt
//
LOCUS       DAZUMK010000066        13732 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000066 DAZUMK010000000
VERSION     DAZUMK010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.068"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(143..1345)
                     /locus_tag="ACG808_001813"
     CDS             complement(143..1345)
                     /locus_tag="ACG808_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5047437.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1348..1542)
                     /locus_tag="ACG808_001814"
     CDS             complement(1348..1542)
                     /locus_tag="ACG808_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFS5047438.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIISVPLSQEE"
     gene            complement(1589..2437)
                     /locus_tag="ACG808_001815"
     CDS             complement(1589..2437)
                     /locus_tag="ACG808_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HFS5047439.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2642..2992)
                     /locus_tag="ACG808_001816"
     CDS             complement(2642..2992)
                     /locus_tag="ACG808_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047440.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3170..4033
                     /locus_tag="ACG808_001817"
     CDS             3170..4033
                     /locus_tag="ACG808_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5047441.1"
                     /translation="MFGLVQPNKVKVGQRIKEIKDGMSLSFTELGNRLGLKKPTISSY
                     VQGYALAPKSVINQLSSISGKPVGWFYFGHVEEYIREYLFLIGQKKIVDDYPEIIEKI
                     KQEFYTGEFKNPAWENELGYPLEEFIQECFVEYSSEIMKDYVVQITKEVLEESKTLNK
                     LSSKAKEEAVTILSSGIIDYIEMSGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSY
                     LIGKLINILGTDEDTELFLSSLSMIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREK
                     TPDEFYDWFEK"
     gene            4657..5649
                     /locus_tag="ACG808_001818"
     CDS             4657..5649
                     /locus_tag="ACG808_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5047442.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5665..6549
                     /locus_tag="ACG808_001819"
     CDS             5665..6549
                     /locus_tag="ACG808_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5047443.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6598..8205
                     /locus_tag="ACG808_001820"
     CDS             6598..8205
                     /locus_tag="ACG808_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5047444.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8206..9690
                     /locus_tag="ACG808_001821"
     CDS             8206..9690
                     /locus_tag="ACG808_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFS5047445.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9867..10859
                     /locus_tag="ACG808_001822"
     CDS             9867..10859
                     /locus_tag="ACG808_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5047446.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10911..11780
                     /locus_tag="ACG808_001823"
     CDS             10911..11780
                     /locus_tag="ACG808_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005472675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS5047447.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12200..>13732)
                     /gene="guaA"
                     /locus_tag="ACG808_001824"
     CDS             complement(12200..>13732)
                     /gene="guaA"
                     /locus_tag="ACG808_001824"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFS5047448.1"
                     /translation="KIIVLDYGSQYNQLITRRIREFGVFSELMSHRITAEEVKAMNPK
                     GIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGK
                     AELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKM
                     YGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLG
                     LSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAK
                     ERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGT
                     ETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPG
                     LGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRT
                     YDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVE
                     WE"
BASE COUNT         4213 a         2321 c         2833 g         4365 t
ORIGIN      
        1 ttgaaaaacg ttgatttatc ggcattcccc atccatatta tatttacgtc ttattcccac
       61 tcaatatgag tagcctcgta aagaagctat tatatcagtg ctttttcagc ctattcctca
      121 ttttggtagg gaaatggtag ggttagattt ttactgcttg atttccatta aagtttgcaa
      181 ctttagattt tgttgcacag gtaaatttaa tgtttcctgt catttgttct gcaattcctt
      241 catcattacg gctccataga tgagcgtagt gctttaaagt gatttcaggg ctagaatggc
      301 ctaaccgttg cgacacgacc agaatgtctg cgttaaactc attgattagg tagctaacat
      361 gagaatgacg taatcctttt ccttgtatta ggggtacctt agctagtttg gcataacgct
      421 gaataattcg ttgaacagtt gaacgataga gtgggaggtc agtgtagcta agaataaatt
      481 gttctacacc atgatttttt tgaataactt tccacttttt tagcacttca attgtatcat
      541 cgtccagaga aatgatccgc aagccgcttt cagtttttgt gtaaggtttc cgaataaaat
      601 ctttttgatt tgtcatttcc aaggtgtgat gaaccctcaa ctttttacgt ttgaagtcca
      661 cgtcattcca attaagtgct aatccttcgc ttacacgaac accagtcatg taatatagcc
      721 agatcattaa aaaacacata tgttcgtaga aatcatcttt ataaattttt gccaaaactt
      781 cttcaaattc ttcttttgtc cagtaaggaa cgattgattt tccttttgga attgcttttg
      841 ttcttttgga aatattctca ttcagaaatt gcagggtgac cgcatagtct aatgatttac
      901 gaaacatacc gtacataagt gaactgtatg cttgtgagta tccgctatcg ttcaataacc
      961 aaattcgata attttcacaa tctcgcacat tgatttcacg aagcttctta tcaccgaaaa
     1021 attcagtaat ctgaatcaat ccacactcac gagaactcca agtacttttt tgtacgctag
     1081 ctttgtagtg tggaacgtaa gactgctcca taaattcttt gtaagttaaa tcataattcg
     1141 caaatccatt tacaaccatg tattcatttt ttatacgcat gacttctttg tgtgcttcac
     1201 gagctgatga gaatcgtttt cctagagaat cttttgttcc tttcttctgg atcctctttc
     1261 cagtcacttg atctgttcct aactcaacta gataagaaaa gcgaccattt ttatcttgat
     1321 acacacaagg gtattttgtt ttcatagtta ttcctcctgt gaaagaggaa cacttataat
     1381 ttcttcaatt acttgtcggg gtactacccc aactctttta ttattataat atggatatcc
     1441 ctgatttacc attcttgttt ttgcttgtcg gattattgtg gtagcttgat gtgttttaaa
     1501 tcctagagcc tctaaatctt ttcgacaaat tgtatttttc attgtataaa ctcactttca
     1561 aagaaaacac tatcattggg taatagtgtt atttctttat ttttgtataa tgaacggtac
     1621 tttcttgaaa atccatcact tgttcaggac ttcccaactc atttaaagct aaagcattgt
     1681 aaaaaaatgg acgtctaact atatattcgc ttgtatatag tatatcttga ttcgtggaat
     1741 catagggttt ctttaccatc tttttggtga cagttggtag tttgagattc cgggacttga
     1801 aaaaagcttt tttcttatgc tctttcaatt ccagctcttt atcgaaatat ttgctgatat
     1861 agcgcccgcg attttcgata ctgtctacat cgatcttgtt gatcttaacg aaaccctgac
     1921 cccaaatcgt agttaaccgg ttaaatgaga taaaggggaa atcaaacaag ataatatggt
     1981 agtggattgc accgcgttgc tgcttttccc acgtggcgag atactttaaa tttgcttttt
     2041 tagttttata gaggtcataa ttcagtcgtt tgataaattt tttaaattca ttatttgcat
     2101 actcaatgtc ttgaatgttt tctttgaaag tgagagttaa aaatttcgtt tgattgtcaa
     2161 aattcgtgtc tacaaggcgg gcaatctcaa atcgcatact ttgataatgt ttctgtttgc
     2221 gttttaaact gtcatattgt ccttgagcgg ataattcatc atagtttttt cttttttcaa
     2281 gagtaatatc attttcataa atccaagcta atttttgttt tcgttccaga tttacttcgt
     2341 tttcgatcgt tttacgtttg ggagtaatga ttgcttcata atcataaatt tcaatatacg
     2401 tgggagtttc aatgattttc tgattataag cttgcaaaaa tcatcactcc ttgttgcgta
     2461 ttcgattgtc aaagaaccag tcattttctc ctgatgttga tctataaatc aagttaaggg
     2521 ggaaggcctg cgtagcagcc cttctagcct atggggtgtt gggcggtctt aacggacaga
     2581 aacggctctg tgggcgctgc cccgccgttt cgtcctaacc acccaacact ccatagtata
     2641 gttagttttg tgtagtggta ccttgttttc tgggaatcaa agttgccgta tcagcggttg
     2701 ccgataaccc ataactgaca aattgtccat tcgagcgtcc ccaaggtaca atcaccaaat
     2761 tggtaaatgt aattatggct aaaccttgtt gggtaagttc ttccggcgtg atcacaggat
     2821 gttcagattt gactttgaca ctgatttttt cgtagcgaag ttttggtagg atacattcgt
     2881 agctccaacc tagaatttcg ccctcctttt gccagcgagt gacattgacg acttggtatt
     2941 ctttttcgag tgtttctttt ggtaaactga gatcggtaat ttttattggc atcacaattc
     3001 tcctttttta tacgaaagtt tatacgattt gattaaaaaa tatagatggc catctacgac
     3061 ttgtattata tacgagaact tatacgtagt caagagattt tctaaaaaaa tatatacgat
     3121 tttttatacg agtgttataa tgaaaaaaat gataaggagg aatttgaata tgtttggttt
     3181 agtacaacct aataaagtga aggtgggcca acgaatcaaa gaaataaaag acggaatgtc
     3241 tctctctttt acagaattag ggaatcgatt aggtttaaaa aagccgacga tcagttccta
     3301 cgttcaaggc tatgcgttgg ctcctaagag tgtgatcaac caattatcta gtatcagcgg
     3361 aaaacctgtc ggctggtttt attttgggca tgtggaagaa tatatccgag agtatttgtt
     3421 tctcattggt cagaaaaaaa tagtggacga ttatcctgaa atcattgaga aaataaagca
     3481 agaattttat acgggcgagt tcaaaaatcc tgcttgggag aatgagctag gctatccttt
     3541 ggaggaattt atccaagaat gttttgtaga atattcgagt gaaatcatga aggactatgt
     3601 cgtacagatt acgaaagagg tacttgaaga atccaaaacg ctaaacaaac tatcatcaaa
     3661 agcaaaagaa gaagctgtca cgattctttc atctgggatc attgactata tcgagatgag
     3721 tggtgagatc aagtatggag agaaagatcg cattgcgaaa atggtgaaag aatcaataaa
     3781 aacctttgat gtgaaacaag acctgacatt taatgattcc tatttgattg gaaagttgat
     3841 caatattctt ggtacagatg aagatacgga gctgtttctt tcttctttat caatgatatt
     3901 tactgataag catttttcca ctcgatttgg tggggaagaa ttagtcgaaa tatttcaatc
     3961 gatgcgacca gcgttaatca aactctatcg ggagaaaaca ccagatgaat tttatgattg
     4021 gtttgagaag taaaataaga aaagtggctt agattacaag ttggtgaaaa aatttttttc
     4081 agcataacat cagtaatcta taaaggggtt gttgattata ctgtagaagt taagtactca
     4141 agagattttt tgtacgtaaa caaagcaaaa aagggtgcag tttcactaga aaatgtactc
     4201 ttttttgtga tttttcttag ttatagagtt tttttgaaac tttaaaaatg agaaaacata
     4261 ttgattttaa agagtgtaga catagcttct tttgctaaat agattaaact tactggtgag
     4321 tggtcttgaa catactttta aatcacattt tactgagttt tattggattt tataattcat
     4381 tggaaacctc gtacttaatc tgtttttatc tatcgactag aatctgtata tattccagct
     4441 atcaatgagg ccgcagaaaa gggtgcttaa aaataatctg ttgtttaaaa tattattatc
     4501 agaatactta gatacaaaat tctggcacaa agtaaaaaaa atattgactt aaaaaaatgg
     4561 atatggtaat ctataaacga aaatagtggt aacgtttcca aaatgcatct tcgttaaatt
     4621 tttatttttt taaacgaaaa tggaaacgtt tctaaaatgt attgggagga gattgaattg
     4681 gaaaagacat tgggaccagg tggtgagata agggcggtac aacgggataa aaaaaatatt
     4741 tcgaagtggg aaaaattaaa gaaacagttg cggcgtgata agtggatgta cttgttgctc
     4801 ttaccaggta tcatcatgac gtttatcttt aagtatgtgc caatgtacgg tgctgtgatt
     4861 gcatttaaag actataatcc tatgctagga attttggaaa gtccttgggt aggactagat
     4921 cactttaggg aatttatcag ttcaccaaat tttacgcttt tgctgagcaa cacattaaaa
     4981 attagtattt atggtttatt gttaggtttt tttccaccaa ttattttagc actgagcttg
     5041 aatcaaatta tcaataaaaa aataaaacag aaaatccagt tgattctata tgcgccgaat
     5101 tttatctcag tagttattat tgtggggatg ctgtttttgt tcctatccgc aaatggacct
     5161 attaatgggt tagtagaaaa ggttacgggg caaccaatta tgttcatgtc taatccagct
     5221 tatttcagaa cgatttatat tttatcggga atttggcaag gaatcggctg gtcatccatt
     5281 ctttacaccg caacgctggc gggagtcagt accgatttat atgaggcagc aaatatcgat
     5341 ggggcaaata ttttacaaaa gatttggcat atcgatttgc ctgcattgag gcctgtgatg
     5401 gtgatcagct tcatcttggc cgccggcggt attatgaatg taggatatga aaaagcctac
     5461 ttgatgcaaa cttcaatgaa catccctgcc tcagaaatca tttccactta tgtatacaaa
     5521 gtcggcttgc aatcaggtga ttatgcctac tctgctgctg ttggtctgtt caatatgatt
     5581 attaatgtcg ttttattact gattgtcaac aatataacca agcggctaaa cgatggtcaa
     5641 ggcttgtaat gaaaggagaa aattatgaat agtgctttat caaaaatcga ccgtcaaatc
     5701 ttatggttga atcgagtgat tgtaactttt ttagtcgtca tcaccattgt tccgctgatt
     5761 tatgtactgg tagcttcttt tatggaccca gctactttga tcaataaagg tataagcttc
     5821 aatccgaaag attggacaat acagggatat caacgggtat ttgcagatga ttccatttta
     5881 cgcggttttg taaattcgtt gttttactct tttagcttca gcttattgac tgtatttatt
     5941 acgatgatca ctgcgtatcc tttgtctcga cctaacttgg cggggaaaaa gattattatg
     6001 actttttttg tcattacaat gttcgtgggt ggtggcttga tacctaccta tttattggta
     6061 aagaacttgg gcatgttaga tagtgtatgg gcgattatcg taccaggtgc tataaatgtg
     6121 tggaatatta tcttagcccg tacctatttt agtgctttgc caaaagaact ttctgaagct
     6181 gctactatcg atggcgccaa tgacttgcaa atactgttca aagtcatgct tccattagcg
     6241 aaaccaatta tgttcgtatt gtttttatat gcctttgttg ggcaatggaa ctcatacttt
     6301 gatgccatga tttacatcaa agatcccgac ttacagccgt tgcaactcgt attaagaaat
     6361 attttgatcc aaaaccaacc ttcacaagac atggtgggag ccaatactgc catggcagaa
     6421 atgaaacaaa ttgctgagtt gatcaaatac gcaacgatcg tcatttctag tctgccatta
     6481 atcataatgt atcctttctt ccaaaaatat tttgataaag gtgttttagt tggttctgtt
     6541 aaagggtaag tattgttcac tatttgtatg aataaaaatt ataaaggaga ttttaagatg
     6601 aaagcaaaat ggatgctgcc aatcgttttt acttccgtac taattttggg ggcttgtgga
     6661 ggctcaaaag aaaagagtaa atcaagccct gattatgaac tagaaaatgt ctcttttcca
     6721 ttgaaagaaa aagtatcttt aaaaatgatg tctcaaagtg cccctttggc tcccaatgat
     6781 cctaatgaca aattgatttt ccaacgatta gaagaagaga ccaatctcca tattgattgg
     6841 acaaactaca gtagtgattt tgccgaaaaa agaaatttgg atatcgctag tggtgattta
     6901 ccagatgcca tcttcaacgc cgctgcaggt gactatgatt tattaaactg ggcggaaagt
     6961 ggcgttattg taccgattga agatttgatc gaagattata tgccaaatct gaagaaaatt
     7021 tttgatgaaa accccgaata ccgacaaatg tctactgcac ctgatggtca catttactct
     7081 atgccttgga ttgaagaatt aggcgaagga aaagagtcga ttcatacagt aaatgggatg
     7141 gcttggatca acaaggagtg gttggataat ttgggtcttg aaatgccgca aaccactgat
     7201 cagttgatga ctgtgttgga ggctttcaag acgcaagatc caaatggtaa tggtgaagca
     7261 gatgaaattc cttttagctt tattaacaat ggaggaaacg aagacttaaa atttttaatg
     7321 ggggcattcg gtctaggtga taatgatgac cacttagtcg ttgataacga tggtcaggta
     7381 caatttactg ctaacaaaga agggtataaa gaagccatca aatatttcaa tgaaatgaat
     7441 aataaaggtc ttattgataa agaggctttc gaacaagatt ggaatacgta tgttgccaaa
     7501 gggaaagaac ataagtatgg cgtgtacttt acttgggata aagcaaatat cacgggtgca
     7561 gatgatagtt atgacgtctt gccagtctta gcgggaccag atggaacgaa acatgttact
     7621 cgtacgaatg gcatgggttt ctcaagagat cgtttcgtaa ttactagtgc gaataaaaac
     7681 ttggaactta cagctaggtg gatagataaa atgtatgaac cgttacagtc tgtccaaaat
     7741 aactggggaa cctacggtga tgaagcacag gcgaatattt ttgaattaaa ggatggtatg
     7801 ttaaaacatt tgcctttaga ggggacagca ccgggggaac taagacaaaa aaccgaagct
     7861 gggggaccat tagcagttct tgattcttat tacggcacag tcactactat gccagatgat
     7921 gcgaagtggc gtttagattt gatgcatgac acgtatttgc catatattac agaagacaat
     7981 atctatccac gtgtcttttt agaaaaagaa gaaacagatc gattagcaaa aatcgaaacg
     8041 gatatgtggg attacatcta tcgcaaaaga gcagagtgga tcaccggcgg taaaatcgat
     8101 gaagaatggg aagaatacct tgctgaattg gaacgtgtag gtttggaaga atggattcaa
     8161 ataaaacaaa ctggctatga taattttttg aagggagcac aataagtgtt agtagaagaa
     8221 acggcgattt tggataaagc aaatcgattt attgaaatga tgcgaggaaa agttaaccct
     8281 cagtatcgac aaaactatca tttttccgca cccatcgggt ggataaatga ccccaatggg
     8341 tttatatatt acaaagggca ttaccatctc ttctatcaat actatcctta cgatagtcga
     8401 tgggggccca tgcattgggg gcatgcgcgc agtagtgatt tggtccattg ggaagatcta
     8461 ccagtagcgt tagccccaga tcaaccttat gacagcggcg ggtgtttctc aggaagtgca
     8521 attgaaagag acggaaaact atatttattt tatacaggct gtcaagaaat tgatggtatt
     8581 agccatcagc atcaatgttt ggcggtctct gaagatggta ttcattttag caaatacagt
     8641 ggtaatcctg tggtagcaga aaaacagcta ggtgaaaatg ggctaataca agaatttcgt
     8701 gatcctaaag taattgttca caacgatggt tactatatgg tagtagctac taaatctaca
     8761 cagtctactg gtagaatagt gatgtttcaa tcgtcagatt tgtatacttg gcacttttac
     8821 tctgtgctgc tagaagggac ttcagagcaa ggaataatgt gggaatgtcc tgatttattc
     8881 catttagacg gacgagatgt cttaatcatg tctccgatac aaatcaagaa gaacggactg
     8941 gaatatcata atatcagttc taccatggct tgtattggtg aggtaaactg ggaaacaggt
     9001 cagatgatag ttgataattt ccatgaaatg gattttggaa tggattttta tgcaccacaa
     9061 acaacaagag atcctaatgg tggacgggtg atgattgctt ggatgcagat gtgggatcgt
     9121 acattgccta cacatgagtt aggccatctt tgggcaggct ctatgagttt gccccgagag
     9181 ttaagagtga aaaaaaatcg attggtacaa aaacccatct caactgtata tagtcattta
     9241 gtttatcaac aaggcagtga aaatattgca gtgactgaca catcagtcgt atttcgaaac
     9301 gcgctgtctg attcttcata tttacatcta gtcgcagatt tggagaaagc tgaagttttt
     9361 acaattgaat tactgcggtc taagacggat tgtctcaaac tagcctatga cactgctaca
     9421 caggtttttg aattggatcg tcagaaacta ggaagagagc ttacaggggc ggaagaagac
     9481 cctctgaatg tacgaaaagt tgaagtacct ttgattgata atcagctcgt tcttgagatt
     9541 tttagagata cctcatcaat tgaagtcttt attaatgggt tagaagttat gacggctact
     9601 ttctatgagc tggaaaaagc tcgagatatc ttgtttcatt cgatcggaga aagtcgtatt
     9661 caagcttttg aaacagcgag tgtgaaataa atgtttgatt gcttaggcga tgaatcaatt
     9721 atttaatagg ttttgaagga taatgttttt agaaaagaac agatagctag atttcccatg
     9781 gatttgctat tagtattttg aaaaaattag taatttatga caaaattcat tatactaaac
     9841 ttgtgcagaa atgtgaggga tcaactatga aagcaacaat gaaagatgtc gcaaaattag
     9901 caggtgttgg agtaggaact gtttcccggg tattgaataa cggttctgtt aaagcttcta
     9961 ctagagaaaa agttgaagca gcaatgacag agttaaacta tcaaccagat ttttatgccc
    10021 gggggttaaa gatgaagcat acgaacacgg ttgctttgat tctacccacg gtatggcatc
    10081 cattttttgg cgaatttgcc taccatgtag aaacagcatt agaaagacaa aactacaagc
    10141 tttatttgtg taactccagt ggtaatagtg aaaaagaaca agaatatatc cagatggtca
    10201 gccaaaataa agtagacggc atcattggaa ttacttattc agatatcgat gaatacattt
    10261 cgagtaatct gccttttgtt agtattgacc gttatttcac tgaagaggtg atttatgtga
    10321 ccgctgacaa tgcggccggt ggacgtttgg ctgcaaatga actttatcgc cgaggatgtc
    10381 agaatgtagc atatatcggt gggtaccaag aaacaccgaa tgaaacgaag agtcgtcgaa
    10441 aatattttga aatcgagtgc cgtcaactgg ggatttccta taaagcgtta gatatgttag
    10501 aacctttaga ccatctctca gaacgtgtaa caaatttttt gttagagaat cccgatatcg
    10561 atgggatttt tacgatcaac gatccaatgg cattagctgt cattgatatg cttgaaaaaa
    10621 tagggaaaaa cgtaccagag gatgtccaag ttatcggatt tgatggtcaa aagatgacag
    10681 aaggagatcc ttatctagtc tcaacgattg cccaacctac aaagcaaatg gcggaagtgg
    10741 cagtggcaaa attaattgct gtcattaatg gagagacagt agaaaaacgt accgtattgc
    10801 cgattgaatt tggcgaaggt gacacaacga aacctttagt gagaaacgat tttgcttagt
    10861 gcaaatacgt ttcttttttt gaaaatgagt gaatgaaagg tgtagataaa atgttatatg
    10921 gtggaataga agccggtgga acaaagtttg tctgtgggat tggtaatgaa caattacaag
    10981 tgatagaacg gaaaagtttt cctaccacaa caccagaaga aacgatggag caagtgtttg
    11041 atttctttga taagtacaaa ggaaatatcg catcaattgg tgtaggagca tttgggccta
    11101 ttgatattca aatcgaatca agaacttacg gatatatcac caacacaccg aagttagctt
    11161 ggcaaaatta tcattttgta ggagctttgg aaaacgcctt gtcggttccc atctattgga
    11221 ctaccgatgt aaatgctgct tgttatggag aatatatggc agggtatgga aaaggtaaaa
    11281 atagtgttct gtattacacc gttggtacag gtgttggtgg tggtgctgta gtggatggga
    11341 aaatagtcgc aggttttagt catcctgaaa tgggacatat gctcttacaa cgtcataatg
    11401 aagatcaatt tgaaggaatc tgtccttttc atcatgattg tttggaaggg ttagcatcag
    11461 gacctgcgat tgaaaaacgc tttggaagaa aagcccagac tatctcattt gatcatcctt
    11521 attggacgat tgaggcagat tatcttgctc agtgtgtgta taacacaacg ttactatatg
    11581 caccggaagt aataatttta ggcgggggtg taatgaaaca gccgcaacta ctcgagttga
    11641 ttcgccaatc gtttaaagaa aaaatgggtt cctacgtgga tacgcctact ctcgaagaat
    11701 atctactaac tccaaggctt gctgacaatg cgggcttgat tggctgccta gctttagcaa
    11761 taaagaaact caatcattag aatagtattt attagaacta ttcataacat accggaagtt
    11821 taaaagggta ttctgaaact taaatagtct caattcattc ttctttcttg agaatctgag
    11881 tagagacttt tattctgttt caaattaata taattacgaa atgatattga gtacaagaaa
    11941 gttcttttta ccagatcttt taaatcgaaa aatctgatat atatatttat agtactaaaa
    12001 atctgattag ataaaaagct aattcaatag gaaaataaag gtagtgaata aaagcaaagc
    12061 ccttgttagt agttaatcta acaagggctt tggtagggaa aatggtagga aaccatctta
    12121 ttttttacga aaatcaggaa ctttagtaat tgaataatgt tgatttatag gtattcctca
    12181 tccatattat gttggctctt tattcccact ccactgttgc aggtggtttg ctggtaatat
    12241 catacacgat acggttaacg tgtgcgactt catttacgat acggacactg attttttgca
    12301 agacttccca agggatacgg gcaaagtcag cggtcatgcc gtcaatagaa gtgacagcac
    12361 ggatacctac agtgtagtca tacgtacggc catctcccat aaccccaaca gagcgaatgc
    12421 ctggtaggac ggtgaagtat tgccagatgt cgcgatctaa accggcattg gcgatttctt
    12481 cgcgcaagat agcgtctgat tcacggacga tttctaattt ttcttctgtc aattcaccaa
    12541 ggacacgaat acctaagcct ggtccaggga atggttggcg ccaaacgatg ctgtcaggca
    12601 tgcctaattg ggtacctaat tcacgtactt cgtctttgaa caatgtgttc aatggctcga
    12661 tcagcttgaa ttgcatgtct tcaggtaatc cacctacatt gtgatgtgat ttgatggttt
    12721 gggcagtttc agttcctgat tcgatcacat cggtataaag ggttccttgt gcaaggaaag
    12781 aaacgccttc ttcaccggca agtttggttg cttcatcatc gaagacatat acaaattcat
    12841 ttccaatgat tttacgtttt tcttctggat cagacacgcc agcaagtttt gataagaaac
    12901 gttctttggc atcgactttt ataatgttca agccaaattt tccgccaaga ctttccataa
    12961 cttgttctgc ttcaccttta cgcaacaata cgtgatcaac gaagatacat gttaattgat
    13021 cgccgatcgc tttttgtaaa agaacaccaa caacagagga atctactccg cctgaaaggc
    13081 ctaataaaac ttttttgtca ccgacttgtt cacgaatttt tgcgatctcc atgtcgatga
    13141 agttatccat tgtccagtca ccagtacatt ggcagacatc tagtgtaaaa tggcgcaaca
    13201 agtcatttcc atattcggaa tggcgaactt ctgcatggaa ttggattccg tacattttgc
    13261 gttctgcatt ttggatcgat gcgatggggc aatctttgct ggtagcaacg acatcgaaat
    13321 cagtcggtgc ttgggtaact aggtcaccat gactcatcca aacggtttgt ttttcaggag
    13381 tgttttggaa aagggccgct tctgcagaag tgatttccaa ttctgctttt ccgtattcac
    13441 ggtttttggc tggtttaact tttccgccta atttataagt aatcaactgc ataccgtaac
    13501 agattcctaa aactgggatc cccaaatcaa aaatcttttc atcaatacca aaggccttct
    13561 catcataaac actgtttggt ccgccagaaa ggatgattcc tttagggttc attgccttta
    13621 cttcttctgc ggtaatgcgg tggctcatca actctgagaa aacaccgaac tcacgaatac
    13681 ggcgagtgat cagttggtta tactgactac cgtaatcaag tacgataatt tt
//
LOCUS       DAZUMK010000067        13722 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000067 DAZUMK010000000
VERSION     DAZUMK010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13722
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.069"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..903)
                     /locus_tag="ACG808_001825"
     CDS             complement(<1..903)
                     /locus_tag="ACG808_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFS5047449.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFSES"
     regulatory      complement(927..971)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1186..2154
                     /locus_tag="ACG808_001826"
     CDS             1186..2154
                     /locus_tag="ACG808_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5047450.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            2267..2545
                     /locus_tag="ACG808_001827"
     CDS             2267..2545
                     /locus_tag="ACG808_001827"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5047451.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            2663..2899
                     /gene="secG"
                     /locus_tag="ACG808_001828"
     CDS             2663..2899
                     /gene="secG"
                     /locus_tag="ACG808_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFS5047452.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3002..3757
                     /locus_tag="ACG808_001829"
     CDS             3002..3757
                     /locus_tag="ACG808_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS5047453.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            3760..6117
                     /gene="rnr"
                     /locus_tag="ACG808_001830"
     CDS             3760..6117
                     /gene="rnr"
                     /locus_tag="ACG808_001830"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFS5047454.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            6139..6603
                     /gene="smpB"
                     /locus_tag="ACG808_001831"
     CDS             6139..6603
                     /gene="smpB"
                     /locus_tag="ACG808_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFS5047455.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            6905..7624
                     /gene="atpB"
                     /locus_tag="ACG808_001832"
     CDS             6905..7624
                     /gene="atpB"
                     /locus_tag="ACG808_001832"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFS5047456.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            7677..7892
                     /gene="atpE"
                     /locus_tag="ACG808_001833"
     CDS             7677..7892
                     /gene="atpE"
                     /locus_tag="ACG808_001833"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFS5047457.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            7983..8507
                     /gene="atpF"
                     /locus_tag="ACG808_001834"
     CDS             7983..8507
                     /gene="atpF"
                     /locus_tag="ACG808_001834"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFS5047458.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            8494..9036
                     /locus_tag="ACG808_001835"
     CDS             8494..9036
                     /locus_tag="ACG808_001835"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFS5047459.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9059..10615
                     /gene="atpA"
                     /locus_tag="ACG808_001836"
     CDS             9059..10615
                     /gene="atpA"
                     /locus_tag="ACG808_001836"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFS5047460.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            10629..11531
                     /locus_tag="ACG808_001837"
     CDS             10629..11531
                     /locus_tag="ACG808_001837"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFS5047461.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRAVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            11554..12960
                     /gene="atpD"
                     /locus_tag="ACG808_001838"
     CDS             11554..12960
                     /gene="atpD"
                     /locus_tag="ACG808_001838"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFS5047462.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            12974..13396
                     /locus_tag="ACG808_001839"
     CDS             12974..13396
                     /locus_tag="ACG808_001839"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFS5047463.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4536 a         2452 c         2991 g         3743 t
ORIGIN      
        1 actttcggaa aaaagacgag tatctacttt tgtcataatt ttactgttag caggtttctg
       61 ccaaaattca taacgatcaa ccaatgtttg tccacgactt attccctgag tttccacagc
      121 ggtgaagctt ttgaaacctt cacagatggc cccctcgatg aaataagcaa cagctaacgg
      181 atcattgatg acacatccta aaattctttc ttgtttccag tggaagtcaa aataaaaacg
      241 agtgatcgct tttaaatact ctccctcttc aggattcatt tgacagcagt attcgaggat
      301 agtcggtgtc aagacgattt ctcttgtcac atctaaccca accatttcta tcgtttgttt
      361 gagattctcg aatacataag aagctgcttc aggatcacac cagtaattgt attcagcaac
      421 tggcgaacag ttaccatggc ttttgtaagt tccacccatc gagacaaaac gatccatatg
      481 tttcccaata tttgggttga ctttcaaagc aaaagcgata ttggtcaaag gacccaaagc
      541 aatgactgat gtatttgtct tttctttgaa ataatcagcc aaaaaatcaa cggcatggag
      601 aggttctgcc tgttttctca agatcatcgg gaaatttgtt tcccctaaac catccatacc
      661 atgagtgtct tgtgcactga tgaaaggttg cttcaaaggt ttttccattc cttgataaac
      721 aggaatgtct aaacgatcac agcgttccag gcatttcagc acattctgcg tacctatatc
      781 tgcagggaca ttcccacaaa ctacggtaat agcaactact tcgatatcag gggatttgac
      841 agctaatgat aaagctaaag tgtcgtcgat ccctggatcg caatcaataa ttacttttcg
      901 cattatttcc aatctccttt ctttagagat acttagtttt ttattctggg aagtttccga
      961 accagtccaa gacgagcgtc ttgattataa tgcacgaaat tagtatactg gcttttattg
     1021 cgaatgtaaa tgctctctta caagtaaatc tattgaggag aactttaagt aaatgataca
     1081 atgaaaaaga aatggaattt acgtgtgccg aaaataaaga atcctatgga tcggtacatt
     1141 ggataataga tagattatag tttggcagaa agcggcgggt gtacaatggt tattttttat
     1201 ttcattctta ttctgataat tatagggatc agttaccatt ttctttctta tttttatgca
     1261 aagaaattag taagagcggg agtacagaga ggatatgact ggtatgaaga cactggtcat
     1321 cgtttgatgg accctgctgc agtcacgtca gtagaaaaaa aacgacaaaa attagaagaa
     1381 gagtgtgaaa gcttttggga gcagggaaca cccaaaacaa tcaaaagtta tgacgggttg
     1441 aaactagctg gtcagatgtt catccatcca aataagcaga ataaatgggt catttgcgta
     1501 catgattacc gaagcacagg gaaaagagac atgtctcata taggtaaaag atatgcagag
     1561 aaaggattca acgttcttgt acctgattta cgtgcgcacg gagaaagtga aggagaaatc
     1621 atcggaatgg gatggctaga tcggctagat ttaattgcct ggatccaact catcttagat
     1681 gaacagccga atgcgtccat tattttacac ggcggatcaa tgggagcttc tacgatcatg
     1741 atggcaagtg gtgaaaaact tccatcagcg gtcaaaggat ttattttaga cagcggatat
     1801 gtttctgttt atgcagaatt ccgatatatg ttaagtaaaa tcactgtctt tcctaaaaaa
     1861 atggtcatga gatatgcgaa tcattatgcc cagaaatacg ccggttactc cttaaagcaa
     1921 gcatcagcta cgagacagct tggcagcaac catctgcctt tattagtcat ccatggtgaa
     1981 agagatcatt ttgtccctac agaagcagcg tacacgattc aaaatgctac ggcaggggat
     2041 aaagcactgc tgcttgttcc agaagcagaa cacttggaag catcgatgaa agatccgaaa
     2101 acctattgga cagtgatttt ttcatttatt gaacaaagaa taacgatgga ttaattttct
     2161 cttctttttt gtaagtaatt cgaggataaa taattgttta tagatctgga gactgctaaa
     2221 ataaggtagg aataaaaaaa tatcagttat ttgaggagtg cggcatatgg aaatcaaaga
     2281 agaaaaaaat cgtcttgctt tattcaatga tgagcaacaa gagattggag aaatgacatg
     2341 gtcagatgcg ggcccagaca tcatgatcat tgaccataca tttgtagatc cagcatatcg
     2401 tggtcaaaag ttagctgaaa agctagtgta tactggagta gaattagcaa gacgagacgg
     2461 gaaaaagatc attcctttat gcccttatgc aaaaaaagag tttgaaaaga aaccagaata
     2521 tcaagacgtc ttacgtcagt cataagaata acagctgaaa gttttgaact ttcagctgtt
     2581 attccattta tatagagttg tcacggactt ttatctatga taaagttaaa gagacgatat
     2641 cttttgggaa ggaggctgcg atatgtataa tttaattttg ggaatcgtga tcgttttatc
     2701 gattgttatg gtcattgcga ttatgatgca gccaagcaag caaaatagtg cagcgagtgc
     2761 atttaccggc ggtgcggatc aactattcgg caaacaaaaa gctcgcggct ttgaagctgt
     2821 catgcagcga tcaactgcag ttatgggagc tatctggatg atcttattat ttgtcctagc
     2881 atatttatct tcgaagtagt tgaataagcc tctcgcaaaa cggcgggcgg ctttttttat
     2941 tgcaaagaga acgcttaaga agaagttatg aaagaaacta tggtaaaatg aggcgaaaga
     3001 ggtgagacga gtgagacaat taccaaaacc cctttatgca aaacatggaa agcgagcggt
     3061 tttgctgctt cacgcttact caggaagtcc aaacgatgtt cgaatgctcg cacgattttt
     3121 ggaaaaagca gattatacag tttatgcgcc gctttttaaa ggacacggaa caatggagcc
     3181 atatgatatc ttgcaagaaa aagcagaaag ctggtgggca gacactaaaa aagccatcca
     3241 ttttttacag tctgagcagt tttctgatat tgccgtatta ggattatcaa tgggcggcat
     3301 ctttgctgta cgagcactag aagaagaatc agtgattggc ggcggttttt tctgttcgcc
     3361 tttgtctcct gtaaaaacaa acgtgcctga aaactttgaa aaatacgtac gtcaagtgct
     3421 gaagacagca ggtaaatcag agaaagaaat caatgaaaaa gcagttgctt accgctcgtt
     3481 agctgaacaa caattaatgg atatacaaga tcaggcagca atcgtagaaa gtaggttgtc
     3541 agatatccaa cagccgatat ttttagctca agctgggaaa gatgaaatga tcgaccctaa
     3601 tggcgtattt gaaactgcac gcaagttgag ccgtcagcga gtgacacttc aatggtatcc
     3661 tgaaagtggt catgtgatta cagttggtac agcaagacgc gaattagaaa aagacgtctt
     3721 ggaattttta gaaaaattac cttggaatga ggaataaata tgacaaaaga aacgataaaa
     3781 gagaaaattc tctttttcat ggaaagcagt cgcaaacgca gtttttctat ggaagaaatc
     3841 gctgaaggtt tagggttcca aaaaagcgat gattttaaat tactagtaca aacgattgct
     3901 caaatggaaa gagaacagag tatagttttc aataaaaaag gaaaagtgaa acttccttta
     3961 aaaccaatct tgatcgaagg aacatttcga gcaaatgaac gaggatttgg atttgtaacg
     4021 attgatcccg aagaagatga catctatatc ccaaaagaag cgactggttt tgcaatggat
     4081 ggagacaccg tagcaatcga tatcatgaag cgagcaaata cagatatgga tcgcggagca
     4141 gaggggaaag tagtcgagat tcgagaccgg gcgactactc agttagcagg ggaattcgtt
     4201 gcttattcag aagaagaaat acaagaaaca gatctttacg gcgttgttat cccgaaagat
     4261 agaaagctca accaatttaa agtatatgca gcagcagagg gagttcgtcc agttgacgga
     4321 agtattgtgc tgatcgaact gacccattac cctgaaaaag ggtatgcgac tagtttagaa
     4381 gggataatca agcaagtgat tggtcataag aatgaccctg gcatggatat cctctcaatt
     4441 gtcgtggcta atggtatccc tacgaaattt cctgatgatg tattgtctga agcagatgaa
     4501 gttccagatc aaatcagcga aaaggatata actggacgta gagatttaag aaatcagctc
     4561 attgttacga ttgatggaga agatgcaaaa gatctagatg atgcagttac ggttaaaaaa
     4621 ttagataacg gtaattattt tcttggagta cacattgcag atgtttctta ctatgtgaca
     4681 caaggcagtc aattggacag agaagcatat gaaagaggga caagtgtata tttgactgat
     4741 cgagtgattc cgatgatccc gcaacgttta tcgaatggta tctgttcact gaatccccat
     4801 gttccaaggc tgaccatgag ttgcgaaatg gagattgatc caaatggaca agtagtctct
     4861 catgatattt tcccaagtgt gatccaaaca acagaacgga tgacctacac agctgtcaat
     4921 caaatcttag aagatcaaga cgaagaaacg atgcaacgct atgctcagct cgtacctatg
     4981 ttccaagaaa tgggtgaatt gcatcgaatc cttgaaacga tgcgaatcaa acgtggcgcg
     5041 atttcttttg aggatcgcga agcgaaaatc cttgttgatg ctgaaggaca cccaaatgac
     5101 atccagctaa gaagcagagg aattggtgaa cgcttgatcg aatctttcat gcttgcggcc
     5161 aatgaaacgg tagctgaaca ttataataaa ttaaatctgc cgtttattta ccgaatccat
     5221 gaacagccaa aagaagaaaa aatgcagcgc ttctttgatt ttgcttcagc tttgggcatt
     5281 ctggtccgag gaacgaaagg aacgatcaca ccaaaagatt tacagcatgt gatcgaagga
     5341 gtagaagaca aaccagaagc tgcagttatc aatactatgt tgctaagaag catgcaacaa
     5401 gcacgctatt cagaagacaa ttttgggcat tatggcttag cggcagatta ctacacacac
     5461 tttacatctc ctattcgacg ctatcccgat ctaatcgtcc atcgtctgat ccgttcttac
     5521 agtgaagata aaagtgaaag caatcaaagt tcgtgggctg cggaacttcc tgaaattgca
     5581 gatcatagtt ctaagatgga acgtcgtgca gtagaaacag aacgtgaagt agattccatg
     5641 aagaaagcag aattcatgat ggacaaagtg ggagaagaat tcgatgggat catcagctct
     5701 gttgtgaaat tcggcctgtt tatcgaactg ccgaatacag tagaaggatt gatccatatc
     5761 aatgaattga aacaggacta cttccatttt gttgaaaatc atttagctct tgtaggtgaa
     5821 agaactggtc tgacttttaa aatcggacaa aaagtgcgcg taaaagtagt caaagctgat
     5881 ccagatgaac gagcagtgga ttttgaactc gtttccgcag aagaggtcac tgcgttggaa
     5941 cgtccaaaag cacgcaatgg aaaatctgga aaacgagaag gaaaacaagc aggcaaacgc
     6001 tcagaaaaaa atggcaagaa gaacaaacga tatagtgata gtaagcaaac gttcggtaag
     6061 aaaaaaggga aaaagccatt ttacaaagat gtcaaaaaga agaaaaagaa aaaataaaat
     6121 gagttggagg aaacagctat gccaaaaggc gagggaaaac taattgcaca aaacaagaaa
     6181 gctcgccatg attattcgat catcgacacg atggaagcag ggatggtctt gcaaggaacg
     6241 gagatcaagt cgatacgaaa cagccggatc aatctgaaag atggatttat tcgcgtccgc
     6301 aacggggaag ctttcttgca taatgttcat atcagtcctt atgaacaagg aaatattttt
     6361 aatcatgatc cgttgcgcac gagaaagtta ttattacaca agaaacaaat cgttcgtctt
     6421 gaaaacgaat tgaaaaacac tggaatcact gttgttcctt taaaagtcta tattcgtaac
     6481 ggctatgcca aggtattgat tggtctggca aaagggaaaa aatcgtatga taaacgggaa
     6541 gatttgaaac gaaaagatat cgatcgacaa attgatcgaa cattaaaaaa tttctctaga
     6601 taatttcatt atcctttttc gcatattcgg tgcttttgct tttacttaat gataaaattt
     6661 gttagcattt aatctgataa aacatgatta tcacaggttt tttcttagat caggagcacc
     6721 gtgcatgggg aaaaattcgc ttcgtttttg actgaaataa aaaagtgtct aattttcatg
     6781 aaaatgtttt gaattttctg tttcatggtg gtatgatacc gtgtggttag aaaccgattg
     6841 gaaaaaacca gatacgcaca tgaattgatt ttatgtgaat ttcggggaaa agaggtgaaa
     6901 tggattggat gaacgctcgc taacgtttca cattggacca atttggtttg atggtacagt
     6961 ttgtataatg gtgttactga cctgtttgat tgttttcttc ttagcttact tttttacaag
     7021 aaatcttaag ttgaaaccaa caggcaaaca aaatgcgata gaatgggtca ttgattttag
     7081 tcgaggaatc gtaacagata atctgcctag aaaagaattg agcaatttcc atttgttggc
     7141 atttacattg tttttatttg tttttgttgc aaacaacata ggtttagtaa caaaaatcgt
     7201 cttacctaac gaaacaacac tttggaaaag cccgacagca gacccgtttg ttactttaac
     7261 tttggcattt ataatgatct tacttactca cttattcggg gtgaaaaagt taggcttcaa
     7321 aggatatttc gtaaattcgt tcttgaaacc ctatagtttt atgtttccga tgaaactgat
     7381 tgaagaattt acaaatttat taacacttgc tttacgtctt tatgggaata tttatgcggg
     7441 ggaagttttg ctaaccttga ttgcaaaaat gatgacaagc ttaggctggt tctcattacc
     7501 attagcaatt ccattagaga tggtctggat cgcgttctcc ttgtttattg ggagcatcca
     7561 agcatttgtc tttgtcactt tgtcaatggt ttatatgagc cataagattg aagtagaaga
     7621 ataatactcg gttaaaaaac aaactactat ttatctattt taggaggaaa acaattatga
     7681 attatatcgc agcagcaatc gcaatcatgg gagcagcaat cggagccggt tatggtaacg
     7741 gtcaagttat ctctaaaaca atcgaatcaa tggctcgcca acctgaaatg tctggtcaat
     7801 tccgtacaac aatgtttatc ggggtagctc tagttgaagc tgttcctatc ttaggggtag
     7861 ttatcgcctt gattctagtt tttgccgttt aattgaatca gtattaaggc agaaacaaca
     7921 tcgtttgtta ctgctttttt acatggaaac tagaaatact ccaaagaaag gaaggctcgc
     7981 ctatgctgaa tcaattggca attgcagaag ttggcaatgc gatgttaggt aatatcatcg
     8041 ttgtcagcgg ctcatttttg atactactgg ctctcttgaa gcactttgct tggggaccaa
     8101 tcagcgatat tttgaaaaaa cgtgaagaca agatcgccaa tgatttagat tctgcagaac
     8161 aatctcgcat caactcagcg aaaatggaac aagaacgcga acaacaattg ttagcctctc
     8221 gttctgatgc agctgatatc atcaaaaatg cgaaagaaag tggagaatta agccgccaaa
     8281 atattttgaa ggatgctcaa gaagaagcag ctcgtctaaa aagcaaagcc caagctgata
     8341 tcactgtaga acgtgattca gcgctgaact ctgtaaaaga cgacgttgca gaactctctc
     8401 ttcaaatcgc ggaaaaaatc ttgaacaaag aattatctcc agaaatgcat gaatcattaa
     8461 tcaatcaata cattgaaggt ctaggttctt caaatgaaac tagataaata tacagtagga
     8521 agacgttacg ggaaagcttt atttgaactg gcgatcgatt caaatcaagc agaagaaatc
     8581 tatcaagatc tcgtaagttt acgtcaaatc tatgaacaag tacctggatt agggaatatg
     8641 ttaagtgatg ttcgtctaga acctcatgaa aaacgagaaa tcatggacaa gcttgtcagc
     8701 ggttatgatg ggatcgttaa aaacttttta gaagttgttt atgagtataa ccgaatggat
     8761 gaccttttgt tcatgatcga agaatatgaa cgtcgatatg atgaacatca agggttacta
     8821 ttgggaagtg tcacaacagc agtcccactt tcagacgaac aacgaataca attagaaaag
     8881 aatgttgcaa aaacaatgga ctatcaatca gttgaattaa aacaaatcgt tgattcttct
     8941 attattggtg gcgcgattgt tgaagccaac catcgagtaa tcgacggaag cattcgcacg
     9001 caattagaaa aaatgcgtaa tcaattaaac agatagtaca aagaggtagg tgaatcgaat
     9061 ggccatcaaa gcagaagaaa ttagtgcctt gattaaagaa caaattgaaa attatcaaaa
     9121 cgtgctttca gtcgaagaaa tcggtaccgt aacttatgta ggtgacggga tcgctcgtgc
     9181 acatggttta gaaaacgcga tgagtggtga gttgcttgaa ttttcaaacg gctcatacgg
     9241 aatggcgcaa aacttagagt caaatgatgt aggtatcatt atccttggcg atttcgagtc
     9301 cattcgtgaa ggagataaag taaaacgaac aggtaagatc atggaagttc cagttggaga
     9361 ggccttgatc ggtcgggtag tcaatccgct aggtcaacca atcgacggac taggtgaaat
     9421 cgttactgac aaagctcgtc cagtagaagc gatggcacca ggtgttatgc aacgtaaatc
     9481 tgttaacgaa ccaatgcaaa cagggctaaa agcgatcgac gcccttgtgc caatcggacg
     9541 cggacaacgt gaattagtca tcggtgaccg taaaacaggg aaaacttcta ttgcgatcga
     9601 tacgatcatc aaccaaaaag gtcaagatat gatctgtatc tatgtagcaa tcggacaaaa
     9661 agattctaca gttcgtacac aagttgaaac attgaaaaaa tatggcgcaa tggattacac
     9721 aatcgttgtg aatgccggtg cgtctcaacc agcaccattg ctttatatcg caccatatgc
     9781 tggtactgca atgggtgaag aattcatgta caacggtaaa catgtattga tcatttttga
     9841 tgatctttca aaacaagctg ttgcttatcg tgaactttcc ttgctacttc gccgtccacc
     9901 aggtcgtgaa gcttatcctg gggatgtttt ctacttgcac tcacgtttat tagaacgtgc
     9961 agcaaaatta agtgatgatt taggtggcgg ttcaatgact gccttaccat ttgttgaaac
    10021 acaagctgga gatatctctg cttatatccc aacaaacgtt atttcgatca ctgatggaca
    10081 aatcttctta gaaagcgact tgttctacgc aggtactcgt ccagcggttg atgccggact
    10141 atctgtatct cgtgtagggg gttctgcgca aatcaaagca atgaaaaaag ttgctgggac
    10201 tttacgccta gacttagcaa gttaccgtga attagaagca tttacacaat tcggttctga
    10261 cttggatgca gcaacacaag caaaactaaa tcgtggacgc cgtacagtag aaatcttgaa
    10321 acaaaaattg catgcaccgc tagctgttga aaaacaagta gtgattttgt atgctttgac
    10381 acatggattc ttagatagta tccctgtaga cagtatttta gacttcgaac atgaattatt
    10441 cgaatatctg gataccaatc attcagatat ctttgaaaca atccgtacaa caaaagatct
    10501 tccagaagaa gaagcgttga acagtgcgat tcaggaatat aaagacatgt tcttagctac
    10561 aaaaggaagt aattcctcta cagaagataa attgaaatcc attcaaaatg catagtgagg
    10621 tgagatgaat gggtgcttca ttaaatgaaa tcaagacacg tatcgcttca acgaaaaaaa
    10681 cgagccaaat cacgcgggca atgcagatgg tgtctgcttc taagttgaca aaatctgaag
    10741 cttcttcaca gaagtttcag gtttatgcaa caaaagtaag agaaatcgtt acgcatttga
    10801 ctgctactca gttaaatgat attgcctcag aaaatcctag aggggatatc aattacaaca
    10861 gcatgctgct cagccgtgct gtgaagaaaa ctggttatat cgtcatcact gcagatggcg
    10921 gtttagtcgg tggatataac agctcgatat tgaaacaaac gatgtctatt ttagaagaag
    10981 accaccaatc acccgatgat tacgtgatga tcgcaattgg cggaactggt gcagacttct
    11041 ttaaagcacg cggtatcaat ctagcatatg agctgcgcaa tctatctgat cagccaagct
    11101 ttgatgaagt ccgcaaaatc gtaaatatgg caacaactat gttccaaaac gaagtattcg
    11161 atgaattata cgtatgttat aaccatcata tcaattcgct aactagtcag tttcgtgtag
    11221 aaaaaatgct gccaatctca gacttagatc cagaagaagc aacgactttc gaacaagaat
    11281 atatttttga accttcaaaa gaagaaatct tgtctcagtt attgcctcag tatgctgaaa
    11341 gtttgatcta cggtgcgatc gttgacgcca aaacagcaga acatgctgct ggtatgacag
    11401 caatgaaaac agcaactgat aatgctgcta atatcattga tgagctgacg gtttcttata
    11461 accgtgcaag acaaggggct attacccaag aaattaccga aattgttgcc ggagcttcgg
    11521 cactagaata gtattgagat tggaggaaaa aagatgagtt caggcaagat tgttcaagta
    11581 atcggtcccg ttgtcgacgt ggaattttct ttagaccaat ccttaccaga tatcaacaac
    11641 gcattagttg tttataaaaa tgacgaaaat aaatcaaaag ttgttcttga agcagcgtta
    11701 gaattagggg acggagtgat ccggaccatt gcgatggaat caacagatgg tttacaaaga
    11761 ggaatggaag tcattgatac aggcaaagca atctctgttc ctgtaggtaa agaaacatta
    11821 ggtcgcgtat tcaacgtact aggagatacg atcgatttag aaacaccttt cccagaagat
    11881 gcggaaagaa gcgaaattca taaaaaagca ccagcctttg acgaattaag tacaagtaca
    11941 gaaattttgg aaacagggat caaagttatc gatttgcttg ccccatattt aaaaggtggg
    12001 aaagtcggac tattcggtgg tgccggtgtt ggtaaaaccg tactgatcca agaactgatc
    12061 cataatatcg cccaagaaca tggtggtatt tctgtattta ccggtgtagg tgaacgtact
    12121 cgtgaaggta atgacttgta ttatgaaatg aaagattcag gagttatcga aaaaacagcc
    12181 atggtgttcg gacaaatgaa cgaaccacca ggtgcacgta tgcgtgttgc tttgactggt
    12241 ttgacgattg cggaatattt ccgtgatgta gaaggtcaag atgtactgtt gtttatcgac
    12301 aacattttcc gtttcactca agctggatct gaagtatcag ccttgttagg acggatgcct
    12361 tctgcggttg gttatcaacc aacattggca acagaaatgg gtcaattaca agaacgtatc
    12421 acatctacga aaaaaggttc gatcacatcg atccaagcaa tctatgtgcc agccgatgac
    12481 tacactgacc cggcgccggc aacagcattc gcccatttgg atgcgacaac caacttggaa
    12541 cgtaaattaa cagaacaagg gatttatcca gcggtagatc cattggcttc ttcttcaagt
    12601 gctttagcac ctgaaatcgt gggggaagaa cattataaag tagcaacaga agtccaacac
    12661 gtattgcaac gatatcgtga attacaagat atcattgcta tcttaggtat ggatgaatta
    12721 tccgatcaag aaaaagtgtt agtttctcgt gcccgcagag tccaattctt cttgtcacaa
    12781 aactttaacg tggctgaaca atttactggt cttccaggtt catatgtccc tgtagaagaa
    12841 acagtgaaag gattccgcga aatcttggaa ggcaaatacg atgatcttcc tgaagaagct
    12901 ttccgaagtg ttggccgaat cgaagacgtc gtagaaaaag cgaaaacact aggctactag
    12961 aaaggggtgc cccatggatc aatatttaac agtaaatgtg gtaactcctg ccggtttggt
    13021 ctatgatcac catgcagcta tcgtcgttgc acgtacgaca gctggtgagc ttgggatttt
    13081 gcctaaacat gcaccaatca tcgttccatt gacgattgat gaagttcgtg taaaacgaac
    13141 agattcagat acccacgtcg attggatcgc tgttaatggc ggaatcatgg aagttcgtga
    13201 taatgttgtt tcaatcgttg ctgatagtgc ggaacgtgaa cgtgatatcg atgtatctcg
    13261 tgcggaacgt gcaaaacaac gcgcagaacg ccaaatcgaa gaagcaaaag caaaacaaga
    13321 tacgaacgaa ttgaaacggg caactgttgc gttgcaccga gcaatcaatc gtatcaaagt
    13381 atctaagcac tcataaaatg agatgcggat gtttatccgt gtttaagaac agtaaaaaga
    13441 ggttatgaca gaagtggtca gcttcaagac ataagaagga atttacgaaa attgcttctc
    13501 aaatttttgt gaaatctcag cttatttttc gaagaagcta cttctggaac accgtatatc
    13561 agaggttgta aaatcagcgt tttgtcctga gtattagaga aacaaatgga aaatagttcc
    13621 tcatattttg ccgttttttg agctaatacc gagggtcaga tgattgaaca ccgtttattt
    13681 gggctctttg tcaaatgatg ttggtaaaga gaaatcttag aa
//
LOCUS       DAZUMK010000068        13584 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000068 DAZUMK010000000
VERSION     DAZUMK010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.070"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(106..1053)
                     /locus_tag="ACG808_001840"
     CDS             complement(106..1053)
                     /locus_tag="ACG808_001840"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFS5047464.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            1243..1512
                     /gene="rpsN"
                     /locus_tag="ACG808_001841"
     CDS             1243..1512
                     /gene="rpsN"
                     /locus_tag="ACG808_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFS5047465.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(1688..2050)
                     /locus_tag="ACG808_001842"
     CDS             complement(1688..2050)
                     /locus_tag="ACG808_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS5047466.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(2061..3182)
                     /gene="alr"
                     /locus_tag="ACG808_001843"
     CDS             complement(2061..3182)
                     /gene="alr"
                     /locus_tag="ACG808_001843"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFS5047467.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(3198..3548)
                     /gene="acpS"
                     /locus_tag="ACG808_001844"
     CDS             complement(3198..3548)
                     /gene="acpS"
                     /locus_tag="ACG808_001844"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFS5047468.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(3678..5189)
                     /locus_tag="ACG808_001845"
     CDS             complement(3678..5189)
                     /locus_tag="ACG808_001845"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS5047469.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(5511..6863)
                     /gene="murF"
                     /locus_tag="ACG808_001846"
     CDS             complement(5511..6863)
                     /gene="murF"
                     /locus_tag="ACG808_001846"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFS5047470.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(7016..8092)
                     /locus_tag="ACG808_001847"
     CDS             complement(7016..8092)
                     /locus_tag="ACG808_001847"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFS5047471.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCAFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            8288..10033
                     /locus_tag="ACG808_001848"
     CDS             8288..10033
                     /locus_tag="ACG808_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFS5047472.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(10313..10750)
                     /locus_tag="ACG808_001849"
     CDS             complement(10313..10750)
                     /locus_tag="ACG808_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFS5047473.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(10734..12107)
                     /locus_tag="ACG808_001850"
     CDS             complement(10734..12107)
                     /locus_tag="ACG808_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047474.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(12429..12917)
                     /locus_tag="ACG808_001851"
     CDS             complement(12429..12917)
                     /locus_tag="ACG808_001851"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5047475.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(12939..13541)
                     /locus_tag="ACG808_001852"
     CDS             complement(12939..13541)
                     /locus_tag="ACG808_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFS5047476.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         3889 a         2944 c         2462 g         4289 t
ORIGIN      
        1 caaagttttt atctattata cgtatacgca aaaaagctaa aacatttttt gtctttgccc
       61 ctacaaaaat gtttcagctt ttttcgtttt ttattcctgt atttcttatt tttcgaatga
      121 caggatgatt ttcccagcag gaggatgtcc ttcaactagc tgatgtgctt ggatgacatg
      181 cttaagtgaa gcagacagat tcattgaaat aaaaacatga aaggctcctt tttggtaggc
      241 tttgattatg ccagcaaagg cttctgcatc tagatattcc tttcttggaa caaacgattc
      301 ataaaagcct tccttttttt gccgcaaatc tagatctggg aggtcattta atgcaacata
      361 cctgccgcct ggtttcatga tctggatgcc ggtttctcct ttgatacttc ctttcgtcgc
      421 atcgattaca aggtctgcct gatcagcaaa ttgcagacct ggatttgttt tatcataagc
      481 agcaaatgct gaagctccta atttttttac cttttcctca tttttactgg atgcactggt
      541 taagattcgg attccctttt catggagaag ctggatcaga cttgacccga ccgcacctga
      601 tgctcctagt atcattacag tatctgtcgg ctggatttcc gtcaaatggt tgattagatt
      661 ataagccgta attcctggcg tgaccattcc ggctgcttct tcccaactca ttttatctgg
      721 aatttttgct acttttgctg atggcaacac gacttcttct ccgtaagtcc cgccgactgc
      781 atggacagct actcgatcac ctacatgaac agttgtgaca tctgaaccaa tctctgtcac
      841 aataccagcg ccatcattac ctggtacata agggaatttc aatgttcgta tctctttcat
      901 ttctcctaaa cgcaaggcaa catcataagg attcacactg aatgctttta tttctacacg
      961 aacatgtcca gaaccgactt ctctgggatg agcatcgatt tcttcaaaaa catcttgtgc
     1021 ttctccgtaa ccgttaattg caaatcttcg catggtcacc atctcccttt ttctttaagt
     1081 ttacgtttat gtctttattt tgacaaaaaa aatgtctttt cgttttggcg catgattcaa
     1141 aacaaaatag ttgtcttttt tcgcgtaacg tgttaaatta tttatctaaa agcgtaaaga
     1201 ttacgattta cgctaataaa aaataattgg aggaatttgt tgatggctaa aaaatcaaag
     1261 attgccaaag caaaaaaaca gcaagaaacg attgaacgct atgctgcttt acgccaggaa
     1321 ttaaaagcgc aaaaagatta cgaaggcttg cgtaaacttc ctttagatgc tcgtccagaa
     1381 cgattgaaaa atcgtgattt aatcgatgga cgccctcgcg gatacatgcg taaattcggg
     1441 atgtcccgta taaaattccg tgaattagca cataaaggtt tgattccagg agtaaaaaaa
     1501 gccagctggt aatccaagtc tggctgtaat gtaaaggagg agggcgcatg gaccactcct
     1561 tggatcatca aaaaagatac gttcgcttct tctagcagaa atgctggtac tagaagcaga
     1621 cgtatctttt tttacgcaca gctggtcctt ttttatatgt aaaggctgca tggttacttc
     1681 gctatattta tttagtaaca ggagtcagtg cttcgatacc cacactaatt ttcaatgctc
     1741 ggtcaacttc tgccataatg ggtcgatcca aatggcagac cttttctttc aatcttgatt
     1801 tatcgatcgt tctgatctgc tccaacagga taacggagtc tctttctatt cctgttccag
     1861 tagctttgat cccaatgtga gtcggaagct taggtttagc catttttgcc gttatggccg
     1921 caacaatgat cgtcgggcta aaatggttac ctagattatt ttgaataacc aaaactgggc
     1981 gtgttccgcc ttgctccgat cccacgactg gagaaagatc agcaaaataa atatctcctc
     2041 tttttaccat aggatcctcc ttattcttga tattctcttg gtatccgctg tcctaatccg
     2101 caagccactt catagtggat cgtacctaat tgatctgcta tatcctgcat agtgatttct
     2161 ttgtctgcat ttttgccgat aagcgtcact tttgttccga caggaaattc ttgaggcaat
     2221 cggatcatca gttgatccat acatacacgc ccaatcgttt cacagtagtt cccttctacc
     2281 aatagagaaa aaccctgcat ctttcgtggc cagccatctg cataaccgat cggtatcgtt
     2341 ccgatccact ctgcttcagg agtgatatat gtttctccat agccgattcc ttcacctgct
     2401 gggagttttt tgacttgaat caattcagaa accagctcaa gtgctggctg caatggataa
     2461 acttccggta gtgcgtgccc agagggatta agtccgtaca ttgctactcc ataacgaatc
     2521 atattgccga ttgtttcatc atgccataaa gccgttgcac tattgctgac atgtacatat
     2581 cgaggaagtt cgggaagttt tttcagcact tctataaagc gttctttttg cagattccaa
     2641 tatgtatcat ctgcctgatc tgctgtagaa aaatgggtaa ataaaccttc ccattctaat
     2701 acacgacttt ctttgatgaa tgcagctgcc tcttttactt cttcaggtgt acaaaatccg
     2761 atacgaccca tcccagtgtc tgcttttaga tgaatcgaaa gaggcgtctc tagcttactt
     2821 ttctctaaaa cagcagctgc tgcttgcaac cattcccgtg tacctgcagt tactgataaa
     2881 tcatgagcaa cagctacagt cagtgcgtcg taaggaacca tattcaagat aaggatgggc
     2941 tgagtaaatc cagcttctct caactcgact ccttcatcta aatttgaaac acaaaagccc
     3001 gaagctccac ctgcaactgc tgcctcagct gtttcaatag ctccatgacc gtatccgttc
     3061 gcttttacta ctgcaaacaa ttcttttcct tgcggaagtc ttgcaacttc atttgctaca
     3121 ttttcagtta tagctttttt atgtatcact gctttcgttg ggcggtgcca agcgacaacc
     3181 atcgtttttc cttctttcta gtcttccaaa ataatttgtg caaaagctgt ttcatttgta
     3241 tgagtaatac tgacccatac attgccttca tgaggtgaac gcgtcacaac aggctgacca
     3301 ttttcatttt ttaaaatctc gacatcttga aaggtaactt ttccgatccc agttccccaa
     3361 gctttagaga aagcctcttt gcaagcgtaa cgcccgccta aaaattctac ttggcgatga
     3421 aaaggcaagg actggaatag tttcatttca tcagaggtca agattcgtgc aatgaatttt
     3481 ggtttttctt cgatcagccg agtgatccta ggaagttcga ctgcatcgat cccaattcct
     3541 tttatcatat gcttttctct tttcaataaa tttagaacac tctcattcta ccaaattata
     3601 accattttgc cacaaataag tccacaaata acaaaaatga aagtttctct taaaaaagag
     3661 aaactttcat tttatgttta atcgttattt gaacggatta caaatccgcg ttttttatca
     3721 ttgtgacgtt taccgccatc tttttggctg ttattatttt tgctataacc gccttttgat
     3781 ttgtttccac gataattacc gccgtcacga cgattacggt tgtttccgcc accacgtttg
     3841 ccatttttat tgtatccttt tttctgcatt ggcaatgggc gttctggtgt gatcttcact
     3901 ggcactgcat cagctggatc ttttgctgtc gtttttagca aaagtgcaac taaatcttgt
     3961 gcagaatatt cttctaataa tttatctgct gtttgcaggt atttatctag accattttct
     4021 gcaagttttg tttcgatttg ttcaactgca gcacctaatt gacctttgaa tgcttctttt
     4081 tcggttggtg gacgtaaagt cgtcatccgt tttttcgtca agttttcaat cacatgcaag
     4141 tagtccattt catttggtgt aacgaaagtc acagacatac cgccttttcc ggcacggcct
     4201 gtacgtccga tacggtgaac gtagctttct ggatcttgag ggatatcata gttgtacaca
     4261 tgagtgacac ctgaaatatc taatccacgt gcagcaacgt ctgtcgctac aaggatatct
     4321 aaatgaccgc tcttaaatga acgcaatacg ctcattcgtt tttgttgaga caaatcgcca
     4381 tggattcctt ctgcacggta accgcgtgct tctaatccgc gtgccaattc gtctacacgg
     4441 cgttttgtac gtccgaagac gattgttaat tcaggtgttt gaacatcaaa caagcgagtc
     4501 atgatatcga atttttcgta ttcttttgcg cggacatagt attgatcgat caagtcagct
     4561 gtcatttctt tcgctttgat cttcacatga tgtggatttt tcatgaattt aacgccaata
     4621 ttcttgatag ctggcggcat cgttgctgag aaaagtaatg tttgtctttg atcaggaact
     4681 tgtgaaatga ttttttcgat atcttccaag aagcccatat tcaacatttc atctgcttca
     4741 tccaaaacta atgtttgaac ggttccaagt ttcaatgtgt ggcggttaat gtggtcaagc
     4801 atacgtcctg gtgtacccac aacgatatgt ggacgatctt tcaagccacg gatctgacgg
     4861 ccaatatctg ctccgccgta aacggcttgt acacgtactt ttttgtcgcg ccctaaacga
     4921 tataattctt cttgtgtttg gatagctaat tcacgtgttg gtgcgataac taaaccttgt
     4981 aattcgtgac gatctggatc gattttttct aacattggta atccaaatgc agctgttttc
     5041 cctgtacctg tctgtgcttg tccaataacg tcttttccag ctaatgctag tggaatcgtt
     5101 tctgcttgga taggcgttgc ttcttcaaaa ccagcgcgtt cgactgattt caacaattct
     5161 ggtgataatt ctagttcttt aaatttcaaa tgaatcctcc tatatggttg ttaactgagg
     5221 atgtagcttt tttgctaccc cgtcgatttg ttcggtcaaa gtgtattgtt tctttttgac
     5281 agtggccacg cacgttttta tccattctta gcccctgtgg caccaccgaa aaatcacttt
     5341 gcgaagttta ggcattagaa aagccaaacg ggtcgcgttc agctttatca ttattatgac
     5401 atctggcttc aacaagttgt ttctatggca aaaccagtcc aatttctctt catctttcgg
     5461 caataggtta ttctaacata gtttttcgtt ttctgcaagt ttacagattt ttatttcatt
     5521 tgctgaagtt gttctaccac ttcaaataag cccatgctgt tgctgccttt tagcataata
     5581 ctatctgttg ctgcaagatt ttgtttgata gcatcaatca atttattctt ttctttttca
     5641 aaatagaaca caggtagttg ggggtatttt tggttcaatt cttcttttag tgcatgcatt
     5701 tgttccccat aaaggaaaat catggcgaat ttctcaggat cgatatgttc actcatttgc
     5761 gcatgcatat ttaaagactc tttccctaat tccagcatat ctgctaacac agcgattctg
     5821 cgtccttttg ttggcagatt tttaaaagta tctaatacca gtcccatggc tgttggattt
     5881 gcattgtaga catcgctcaa aatagctgca ccatttcctg ccgttagcca ttcggttctg
     5941 ttttgagtga tagaaacatg ggcgagccct tggcgtatct caggtatgga cagtccaaag
     6001 aagcgcccga tcgtatacgc aatcaaagca ttcgttacat tataacttcc aggcagcggg
     6061 atcgtaaatg tttctccttc tactacaaaa tcggtttgtg cttttccttt tgctgtcact
     6121 tccgcatgaa catcgccatt tccaataccg aatgttgtga ctgtttgtac taatgttgcc
     6181 gttaaaggtg tcagcaacgg ttcatctgcg ggcacaacga gtaatccgtc tgatacaagt
     6241 ccgtcagtga tttccatttt ggctttagcg attccttctc gcgacccaag attttctatg
     6301 tgtgcttcac cgatcattgt gattgctgca gcttttggtt gagccataag tgaaagttcc
     6361 gtgatttcat tggcgtgatc catgcccatt tccaaaacga tcttttctgt atcttggggc
     6421 atgtgcaaaa tcgtgtaagg aagaccaata ttattgttgt aatttccttg cgtcttatat
     6481 gttttgtatt tttcagacaa gacagacgcg gttagatctt tcgtggtggt tttaccattg
     6541 cttcctgtaa ttgcaacgac ttctgcgcct actttttcca agtaataggt tgccaattgc
     6601 tgcatggctt tcaaaggatc tgctacagga ataactggaa aattttttgg agcttgttca
     6661 acaggcaagc tccaaaatga agcagatgct ccgtttgtga tcgcttgctg gataaattca
     6721 tgtccatctc gcgtaccagc aagaggaacg aataaacttc cttcggttat cttccggctg
     6781 tcaaattcga cattggtaat ctttgtcagt gcagagcatt cgctgcccac ggcttgtgca
     6841 atttcttgta ttgttagatt cattgagatc tctcctaaaa aaggttgtga aaaaagcgtt
     6901 tggcctaaac ggcgggttcg catgactctc acttccaagt aaatttctct attcgtattt
     6961 agaagctaga atgaacgcgg ttacgcaatc actccagcct cttctttatt tctctttatt
     7021 catttttttc aaaaaaagat tgacgctgat ggtatcgatt cattcctaac tggatcagtt
     7081 cttcaatcaa atcaccgtat ttcaagccca tattttccca taagagtggg tacatactga
     7141 actccgtaaa tcctggcata gagtttaatt cattcaggaa taattcattt ttatttgtca
     7201 aaaagaaagc gcaccggctc aatccgcttc cacctaacat cgtgtaagct aacttcgcgt
     7261 actcttgcgc tttttgataa acttcttctg gcacttcggc tggaatctgc atttcgattt
     7321 tattattgat atattttgct tcataatcat agaatgctac gtcttttacg acttcaccag
     7381 gcaaagtcgt ccgaacgtct tcatttccta atacagcaac ttcgatttcg cgcgcttcaa
     7441 ttccttgttc aacgattgct cgagaatcat actgataggc tgttgctaaa gcattttgca
     7501 gctcttctcg gttttctgct tttgtaatgc cgacactaga acccatattc gccggtttga
     7561 caaacatcgg ataaagcaaa gaaccttcac attgatcaaa tactttttta ggattttctt
     7621 tccattgatt cttaagtact ggtacataag gaacttgcgg cacaccagca gcttgtaaaa
     7681 tatacttggt catgatttta tccatggcac atgcactggt caatacgcct gcgccgacat
     7741 aaggcatatt caatgtctct aagaagcctt ggatcgttcc atcttccccg tttggcccat
     7801 gtaaaactgg aaaaacgatg gctccttctt ctttgatttc gcccggattg atcacttttc
     7861 ctgtaaagcc ttcctctgtc tgtccacttg ggtcccatga aagatgcaag acatctttgc
     7921 tggcaggttt ttctgttagt aatggacctt tgacccattg tccttctttt gtaataaata
     7981 cgagttgaac ttggtaataa ttataataaa tggcatttaa aactgaaaat gcggaaagaa
     8041 tggacacttc atgctctgcg ctgcgtccgc catatagtaa agtaatcttc aaaagattgt
     8101 cctcctgtat tctaaatcgt tcagtgattt actctcatat tttagcacaa aacaaaaata
     8161 aacgaataac ttatttataa aaaaacgagc cagcttctaa aagaacgagc gtcatttatt
     8221 ttttgtcagc tagattattt gtcccgtttt ttataaaaag ttatgataaa gaaaaggagg
     8281 gatgatgatg gcgcaaacaa atccacgcag ccgcttggca gaaatcattc gagtgttcat
     8341 caattacaat gtagtgccaa actttgtaca gcaaaaaaat cccgaacagg taaaaaaagc
     8401 ctttgaagaa ctaggcccca ccttcatcaa gataggacag atgctttctg ttcgagaaga
     8461 tttgctttct tctgccttca ctcaaacatt caaaacatta caagatagtg tacctagcga
     8521 cacattttca acggtcaaaa agacgatcga aacagaactt tctctttcat tatcagatat
     8581 attcgatgat ttttcaaaat cgccttttgc ctctgcttct atggggcagg ctcatcgtgc
     8641 caagctgaag aacggtgacg cggtagtcgt taagatccaa caccctaaca tcgccgaaga
     8701 gatccgactt gatcttcaac tatttgaacg agcgatccct ttgatcaaat acattcctga
     8761 aactagcgta gttgatctaa aaggggtact ccaagaagta aaacgttcct tgatcaatga
     8821 aatggacttc ttaaaagaaa gtcaaaatgg cgaacaattc tatcaaaaga acaatggctg
     8881 gaaagagatc cgcagcccca agatttatga cgctttttgt tcgaaaaaag tgatcgtgat
     8941 ggaagaaatg tctggtaaaa acttgaatca tttaatgaac gccgaaaaca aaactgaaac
     9001 ttttatcact ggaattcaaa acaagcagct gaaacaagaa gttgcaaagc ttttagttga
     9061 gaactttatg aaacaggtgt ttgatgatgg ttttttccat gctgatcctc atccagggaa
     9121 tctgctattc catgtactaa caaaagaaga acagacacaa gctagccgaa aaactgaaac
     9181 agtccatgaa aaagagttcg gttcctttgc ttttcgagcc tccacttctg cagaagaccc
     9241 tgttgcgcca tataccatca actacatcga ttttggtatg atggggcatc tttctgcagg
     9301 acttaggcaa aaactcacac aagccgtttt agcgctgtat acgaaagatg cttatcgaat
     9361 cgaaaaagcg gtccttcgac tttgtcagca agaaggctcg tttgatgaaa gtcggttcca
     9421 ccaagaactg acaagttttc ttgaacagta ttatgattct cctattgatg aaatcaactt
     9481 gcaagaagta ttcacacatg tcgtgacgat ttgtcatcaa aataatctgc agttcgatcg
     9541 tgatatcacg cttttgctga aagcatttgg gacattagaa ggggtgatcc gcgtcctcga
     9601 tcctgaagtt tccctcatgg aagtcgcttc gccttttgct caacattatt ttctcactca
     9661 tctggatgta gaagatacat tgaaacaaag cggattagac ttattggaag ggatgaaggc
     9721 tgcgcctaag atccctcaac agctccacca tctattagaa atgtggactt cgggacaagg
     9781 aaaagtcaat ttggaattga aaaagcaaga caaattattg agcaggatcg aaagtatgat
     9841 caatcggctt gttttcggta tgattttagc tgcattgatc gtagggtcct ccctgcttgt
     9901 ccaagcagca cctgtagaaa acgcagaggt cgtttcctta ttagggatat ttacgtatgc
     9961 tattgccgca ttcgtcatta tttttttggc aatcgatgca ttgatccaaa tgtataagaa
    10021 gaggaaaaag tagaaaaagg ttgtgacaga aagaatcagc ttcaagaaat aagaaggggt
    10081 tttacataaa ttgtttctca aatttttgtg aaaattcggc ttatttttcg aagaggctac
    10141 ttctgaaaca ccgagaataa aaaatgtgaa aaagctgttc tgcacccagt aataagaaca
    10201 atcaaaaagg gaacatggaa cacatttatc ctgtttccct gttccctttt tgatgtattc
    10261 gagcataaat aaaatacctt cgttacctac tatcttctgt acttggtaaa ccttagtcaa
    10321 ttgccttttt taacgcttga cgaaaagaca tatgatcttt ctccgtaaag ggttttggac
    10381 cctttgtcct tatgccagct tttcttaact ttccactatg ataacgttgt tctagaagcg
    10441 tgccgatatt cgcttcgtca aattcttttc cagagtgatg gaacactcgc acacccttcg
    10501 gcaatacaag tgaagctaaa ccataatctt gcgtgatcaa aaagtcattc ttcttaacca
    10561 taccgacgat tttataatcc gcactatcag ctccgcggtc tacgtagacg atttggacat
    10621 gattagcgta ttctttggtc gtataatggt ccacactggt cacgataacc acgtcaagac
    10681 cataggattc tcctaatttt atgacatctt cttttactgg agaaccatct ccatcaatga
    10741 atagcctcat tctttttcgc cttcttccat aaatgataaa cagttactaa tccgctcagt
    10801 aaaagaccaa cattgatcca ttttgccaag ataagcagtt gtacactttc tatcggcact
    10861 gattcatggt tcagcgtgat caataataga tggattggct ctcccatttc ttttgctaaa
    10921 acaaacaaaa gcgtcatcaa taaaaactgg tggatcacac tccctttatt ttgagtagta
    10981 tacttgacaa cgatccggcg atccattgga atagctgtat cagtttgata atattcctta
    11041 tttagccagc tgcctgttac tacgtgccag aaactaagca gaaagacacc tatcgcaaaa
    11101 ccatttggaa tcaagaggac caataaagaa gaactcagtc caagcaattg catagccaat
    11161 ggagaccatg ttaaacgttt caaaatccat ggtccgacta tttttgctgc aatcaagcct
    11221 gctgcataag gcaaatacat aaaaatcgaa agtttttcta atccgtacag tatgccaaca
    11281 taaaaactgc taaataaaaa aaggaaattc cctagcattc catttagaaa agtaaacaga
    11341 tttagccagc ttggcaactt ccattccttc ttctggtgtc ctaaaacaaa cacgaacaag
    11401 atgaacagca gcaagaaacc ataaatagca agatcaaaaa gcctttcatc gaataacaat
    11461 cgggcatttc tcaaaagaaa caataagcca aagaatatca aaaataaaac aagctctcgc
    11521 gtagctacca gatttctttt gatgtctttg aaatccagtt cgtaacgcgg ataatgacta
    11581 accgtatggt aggccatgac gtaaaaaagt gtataaatag taaagcttgc tgcaatctga
    11641 gtcggtgcag gcaactcaat cgcttgatac aggagaaata gccagatagc tgcaaataag
    11701 tactttttcc catccatatt gataaaacct tgcgtctttt cgtgaaaatt gaccgtggta
    11761 ttagcaggta caagccatgc tgcactcagc cctagcaaaa cacttgaaaa ataaaataaa
    11821 ggaaaataaa atacgcctaa tattccaagc aatgaaccag caccaccaat cagcagagcg
    11881 atcatcagca acgtgtaact atctagacca aatcgtagac tgttgactaa aaatagtcca
    11941 gtcatccgaa aggcataaaa cagcgtaaat ggcaaaatcg tcagccaatt atgcgagtct
    12001 gttagtccca aaaataaaag ataaggaata aaaataaccg tgttacttaa gataaagggc
    12061 gttgtttgtg taaaattacg cccaaatcgt ttgagttttt cgaacatgac aatggctcct
    12121 taaacagtct tataactaat ctgctcttcg attttagcat taaaggggaa ataaaccaat
    12181 tttacagaat atattcttgt aaaaaaaaat acagaaagaa gcataagctt ctttctgcat
    12241 ttttacggta cttgattact caagcagaca agcccttctc cacaaaagtc ttgcccgaca
    12301 cccctgcatc cggcaggtga taaagataga aatcaattat cctgtgcctc caaaatcaat
    12361 taggagctct gtaaaccaga gtccttctcg acccgtcgca taaatttact ttaacacagt
    12421 tcctatgttt atgcgagagc ttttttcatt ttataatcga ccatttgaaa tccttgaccg
    12481 atatctgaaa taagttcttc tgtaatctca aatccttcat gcttataaac ctcgattgct
    12541 tgatgattcc cctgattcac tcgcaaaaag atctctcttt cctttgtttg cgcagcaagt
    12601 tcttcatacc agcgaaaaac tgaagaagtc aatccttgac cacgtacttt atttagcaaa
    12661 tataatttac ttaaataaat ttttccattt atctcttcat aagcagtgta accgacagtc
    12721 ttttcttcgc tttttaataa aaagtaggac acaccttctg tcagttcttt ttgaatctgt
    12781 ttttttgact gataagtttt cagcatgtaa tccacttgtt tttgaccaat gatcggcaca
    12841 aaagcttccg gccatacttc ttgtatgact gccatcagct catccagttc attttctgtt
    12901 acttttattt tttccataca cccacgtcct tttttacatt ataaatgacc ttgcgcttga
    12961 tggaataaaa agagcatgat catgctatac acacccaccg accatggttt caagaaaatc
    13021 acaatctgca taaattccct aaaagataat ttcgtcaaac cagctactag acagatcaag
    13081 tcgtctggcg caaagggaag cagcaaggta acaactagta cttttttaat atctttcgtt
    13141 tttcttgtca gcaattcatc aaatttctga tacttttttg gcgataaaat aagctggaca
    13201 aaggctacgc catagtaacg gacaagcaaa aatccgattg cttccccaat cactaggcca
    13261 atacaactat ataaaagccc aaatccattg ccaaacatca gcatgcccgc tactgaggag
    13321 atccctcccg gcaaaatagg aatgatcact tggatcactt gcaacagaac aaatatcatc
    13381 actgcatact tgccaaattg ctggataaac gcttgcaaag acgtgctcga tcgaaaaatc
    13441 ccatgatgat aagcataaag aatcaacaca aagaaaaaaa acagaccaat aatgggcatg
    13501 atctgaagac atttttgtaa aactgatcct tttttagaca tctatattcc ccacatcttt
    13561 tttcatttat taaaactgcc tcct
//
LOCUS       DAZUMK010000069        13453 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000069 DAZUMK010000000
VERSION     DAZUMK010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.071"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            140..307
                     /locus_tag="ACG808_001853"
     CDS             140..307
                     /locus_tag="ACG808_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFS5047477.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(369..2180)
                     /locus_tag="ACG808_001854"
     CDS             complement(369..2180)
                     /locus_tag="ACG808_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFS5047478.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(2177..2542)
                     /locus_tag="ACG808_001855"
     CDS             complement(2177..2542)
                     /locus_tag="ACG808_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFS5047479.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2705..3100)
                     /locus_tag="ACG808_001856"
     CDS             complement(2705..3100)
                     /locus_tag="ACG808_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFS5047480.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(3118..4080)
                     /locus_tag="ACG808_001857"
     CDS             complement(3118..4080)
                     /locus_tag="ACG808_001857"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFS5047481.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(4080..4292)
                     /locus_tag="ACG808_001858"
     CDS             complement(4080..4292)
                     /locus_tag="ACG808_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFS5047482.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4313..5011)
                     /locus_tag="ACG808_001859"
     CDS             complement(4313..5011)
                     /locus_tag="ACG808_001859"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFS5047483.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(5031..5573)
                     /locus_tag="ACG808_001860"
     CDS             complement(5031..5573)
                     /locus_tag="ACG808_001860"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFS5047484.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5705..6709)
                     /locus_tag="ACG808_001861"
     CDS             complement(5705..6709)
                     /locus_tag="ACG808_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFS5047485.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6706..7695)
                     /locus_tag="ACG808_001862"
     CDS             complement(6706..7695)
                     /locus_tag="ACG808_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFS5047486.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7692..8498)
                     /locus_tag="ACG808_001863"
     CDS             complement(7692..8498)
                     /locus_tag="ACG808_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5047487.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8664..9620
                     /locus_tag="ACG808_001864"
     CDS             8664..9620
                     /locus_tag="ACG808_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5047488.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9697..10215)
                     /locus_tag="ACG808_001865"
     CDS             complement(9697..10215)
                     /locus_tag="ACG808_001865"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFS5047489.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10303..10452)
                     /gene="rpmG"
                     /locus_tag="ACG808_001866"
     CDS             complement(10303..10452)
                     /gene="rpmG"
                     /locus_tag="ACG808_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFS5047490.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            10680..11126
                     /locus_tag="ACG808_001867"
     CDS             10680..11126
                     /locus_tag="ACG808_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFS5047491.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(11321..13216)
                     /gene="asnB"
                     /locus_tag="ACG808_001868"
     CDS             complement(11321..13216)
                     /gene="asnB"
                     /locus_tag="ACG808_001868"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFS5047492.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         4197 a         2841 c         2294 g         4121 t
ORIGIN      
        1 atatggtaaa tttataataa aacatttagt aaacattttc aaggattttt tgcaaaggtt
       61 tacaaacaaa cgtaaaacta gccttttttt ctcttttgtt ccataataga tataacaaat
      121 aataaatgag gtggattcaa tgaaaaaatt tgtatctgga atattagtag gaagtctggc
      181 aacagccgct gccgttgctg gtttggtcgc ttctgtgaaa aaaacagtga tcgatccaat
      241 tgacgaaaaa gaagcaatga tcgaagaaaa ccgtaaaaaa gcaatgcgca aacgcgtttc
      301 tcgttaataa ttatttaaaa acacgcatga agacttgtga aagaacagat aagtcatcat
      361 gcgtattttt atttgtcagt caatataagt tgtgctattt tatctgcctg tctcagattc
      421 catggatctg tataaggtct agctactgct gcagttagcc aatccacacc ttcaacaggt
      481 attccatagc aataaaagtc agactggatt tcccctgaag catcaatcac gcgattcgta
      541 cctcgctcaa catctatagc ttctgaatga taacttcctg tattatctga aaatgaaaaa
      601 gggcgaacaa gtttgttttc cttcaaattt tttatgacag gattacttga acgagaaaag
      661 cttgtttgtg gcaatcgagc ttcaatcaaa tagcgactgc tgatttcttt atcttgtaga
      721 gaaataacat agcggttgtg cttttgctga atatcaaatg gcggttcgaa caaatgaaaa
      781 atacccgcat ctatcagtgc tgctaattct ttcgtccgaa tgactggcgg accgatcgtt
      841 aaaaagctat ttagatgagt aaaccatccc catagtattt ctttgtattc ctttgcagaa
      901 aaaacgtgcc attcaatagc ctgccggatt ggatcacgcc agtcttttaa tgcatcaaat
      961 gttgaagcaa atgctcctgt gcaattgcct ttttccgctt cttttatctg cccttcaata
     1021 aatgctcggc tagcttctgc aaaggactca tcctgatagg taagaggaat ttctaaatac
     1081 gaccaatccc atctataagg gattagtgct ggatgctttc cgagacactc ctctgaagag
     1141 agggagagaa aatcatgctc aaaatttttc tttggaatcg gcaaatgata ttcttcgatg
     1201 attttcttgt aataaaacaa ctcaacgtct tttttcagat agtcaaaaaa tgtttcgcca
     1261 gtcaaaaatc ccgactgatg ccattgctcc aggttttctt ttgttagtaa ccgtggccat
     1321 gccattgatc cgccttgctt ttggtttctt cctcgagggt aataagggag cccccttctt
     1381 gatccactgt atacaatcgg ttccttgcct gatggatgat agacaagttt tcctttttcg
     1441 ttctcgaagc gcccttctct tgatactgta aacaatccga cataatcaaa aaaacttaag
     1501 cctagacctc ttaaaaagac attttcttta ggcggtatct tcgaaagatc gacatctgca
     1561 ggattagaag gaagctgata cagtaaatca tttttctttg cgtagtctac ccacactctt
     1621 tcttcttctg tctcatcatt ttcccagtga ccgcttgcta aaacaagctg atcgatagaa
     1681 tagctttgct gatctgtctg taaaagataa ccatcttttc ttttgctgac tttttttact
     1741 aaagaataaa caaactgaat agaagctgga aattcttctt gcaactttga gaaaaaccag
     1801 cgttgataga gtccgtataa acatcgagta gattgctgat ctttttccag acgattgatt
     1861 tcttctataa ttagttgctt atttttggtt accgtctgtt cgatataatc tgtagcaggt
     1921 cctttactcc attgaaacaa gttgggtccc tgtgttacca atcctccact ggacaatgtt
     1981 tcatctgtaa aaagcgtcac ttgttgagaa acagaattca ttaataactc cggtggctga
     2041 ttcaaccgcc aaactcgacc tccaggacca ttgggatcga aaagaaaaag ttcaagagaa
     2101 tgtctgtcct gattattatg gagaatcctt tctgctatac ttaatcctct aggtccggca
     2161 ccaatgatcc ctattttcat ttgcatgcct ccatttcgat aaattttttc aataaaacag
     2221 catggttctg atgttcattt tttgcaccat aaacaaaagt aatgactgga tgttctttca
     2281 taagttgact gagtttaaaa atagcttgct tcttttcttc ttcgaaaatc aattcttgct
     2341 ggtatttctt tgcaaatgaa gcaaatcgtt ctggtacatg gtcaaaagtt ttgcgtgctt
     2401 cattactagg tgcaatttct ttcaaccaca gatctaactg cgctttttct tttgaaattc
     2461 ctcgtggcca gaacctatcc acgagtattc tatagccatc ctctttttca gcatcaaggt
     2521 aaacacgttt taatttgatc atagttgttt ccttctttct gccttttcgc ttagcttata
     2581 tatgtagata atagcacaaa ataaaaaaaa tgaacggcac aactatccat tgcgccgttc
     2641 atgtgatctt ggtcaaaatc taatgattga ttcggttttt aaagctgtca tacaatcatc
     2701 tgttttatac ttttttactt ttgatttttc cagaccatcc gtcatatcca cccttaagta
     2761 aataaatctc ttgataacct gctttacgta atttatttgc tgtacggatg ctcaagcttt
     2821 tcttttgatc gtaaatatag acaggctggt cttttcgaat agatcctaat gaatctttca
     2881 aaacagtgta agggatgttt cttgcaccta aaatatgccc agcatcaaac acatcttttt
     2941 cgcgaacatc gattacttgt gcttttctca ttccttctcg aaattcttct tctgtcaaag
     3001 tcgttgctga acgcttggcc attactcgta gatagacttc ccaaaagatg attgcaagca
     3061 caatcaatag caaaatgata ttaattaccc ataaaaccat gtaatgcctc cgttttttta
     3121 agcaaatttc aaggctaatg aggcagcacc aatgacacca gcttcattac caagctctgc
     3181 taatttgatt tttgttgatt ctcttacttg cgggaaagta aattctttaa aatatttctc
     3241 tacacggcta cgtaaaaatt ctcctgctgc cgatactcct ccacctaaca caatacttga
     3301 cggattcaat gtattcccca agttggcgca agctaaacct aagtagaagc atacgcgatc
     3361 aacaaccatc aatgcaaacg gatcgtcttc ttgagccatt tcaaagatat ctttacttgt
     3421 tacttcttca ccgttgtcta acattgcttt cagttttgaa tctcctgcgt attcttctgc
     3481 caagtaacga cctagacgta ccacaccagt agcgctagac acagtttcta aacatccgcg
     3541 tttaccgcaa gtacattcga acccttcagg atccacagtg atatggccga tctctccgcc
     3601 tgctccgctc acgccgtgaa tcaaatttcc gccagcaacg ataccaccgc caacacctgt
     3661 tcctagagta acgaatacaa cgtctggatc gttttctcca gcacctttcc atcgttcacc
     3721 taaagcagca acattcgcat cattatcgat agcgaatttg attcctgttc ctgcctcgat
     3781 cagttttttc acaaattgaa cttctgtcca gtttaagtta tacgcaccaa taaccgttcc
     3841 agcttcgctg tccacgcttc ctggggtacc catgccgatt ccgataaagt cttcggcttt
     3901 catattatat aaattcaaac gatggttgat tgattcaatg atttcaggaa cgatatgctt
     3961 accatcatcc aaaatattgg tatcgatgct ccatttttgt tgaatctctc cttctggtgt
     4021 taaaattgca aattttgctg ttgttccacc taaatcaatc ccaataatct tcttgtccat
     4081 tacaaatttc ctttctttcg gctttcttca attcggtgct ctctttttaa tatgcgccac
     4141 gctgtcatgt atgtatcatg atcaatcaaa cggctatcat acagattacg caattcgata
     4201 gtcattaatt caatgtcata gatgcgtgca cccacataga taaagatgcc aaattttttg
     4261 aacagctgct gtacatcata taagctctcc atctttaagc acttccttca tattatacaa
     4321 gtaaaccata tttttttaaa cccagtaaaa tacatattac aaaaagaaat acaaaaacta
     4381 gtgtagaaag aatcctttgg tgtatcgaat agcttgtctg ttgtttcggt agacccaaaa
     4441 cattccctaa cactaatccg ccaattagcc caccgacatg tccccaaaca tctacagagg
     4501 tatccagaag accaaaaaca aatgtcaaca caataaataa aagaaaatgt ctgactagct
     4561 gctggatcgc tgggttatgt ttaaaatgaa accctaagat aaacaacgca ccgaacaaac
     4621 cgaatatcga agtactcgca ccaccagaca gaacattcgc ttcgttaaaa gcaaagcttg
     4681 ctgtatttcc cattatcccg ctgaacatat aaatcaagaa aaaacgccaa tggccataga
     4741 ttgcctcgat ttgctgcccc ataaaataca gtatcagtga gttcattacg aaatgcatca
     4801 gtccaaaatg gatgaagatt ggtgtcacta aacgccacca ttcattgaag tgaacaagat
     4861 aaggaccata cattccggca taataaggga tctcaagtcg cggaaataat tccatcatga
     4921 ggaagaccgc tgtctgtatc cctaaaaaag cataggtcca taacggtcgt cttaaccatg
     4981 cacgcaattt tatttgctgt tgataattca tgaaaacctc ccatttcatt ttagtcaata
     5041 aacaattgtt ggatcttctg atcaaagctc tccggttgcc attgttcatt caattgttca
     5101 cgaaaaacaa ggctacatgt ttttttatga aaattattta aataccggtc gtaataacca
     5161 ccgccaaaac cgattcgata accggctcta ttgtagataa tacctggtac aatgaggaga
     5221 tcgatcatct ccggcaaata gagcttctct gattgtggct caagaacacc aaaactcgaa
     5281 cgtacaagaa cggactgttc atccatttca taaaacgcca tctgacgttt cggcaaagtt
     5341 ttaggtacaa ctattttctt ttgttctttt tctgctcgtt ggataaccgg ctgtgtatcc
     5401 agttcaaatt cattcgataa agtcgtccct atcacttttg ctgacttcca gtactcgctg
     5461 gcgaatagct gtttatagat tcgctgttct ttttcctttt tcttgccact tttggccaga
     5521 tttttcagct tttcgattcc tttccggcgt aatcttactt tcaattggtc caaaaactca
     5581 cccttccttc tctcataaaa tggtatagga aaaaaagaaa aatagcaatc gaactttcaa
     5641 ataaatgcat aaatagaata aaaagaagac ctagaaatga ttggccttcc ttttttaata
     5701 aatttcaact gttcagcgcg tgtttttgtc tgataaaaag tgcatacaga aaatatggcg
     5761 caccaaaaag ggcaatcaaa attcctgtag gtacttcccc acttggtaga atcactctag
     5821 caaccatatc tgcactaagc agaaaaatgg aaccgaacaa accgctcaga acaaaggtcc
     5881 agttattttc tttacgtacg actagcttcg cagcatgagg agctagtagt cctacaaaag
     5941 ataaactacc tgtcaccgct acactaccgc ttgccaaagc aacagctaac aacaaaagga
     6001 ttttttgact tttagccacg ttaaggccta atccttgcgc gcgttcttga ccaaatgata
     6061 gcaatccaat cctctgtgca taaagtcctc ctagcggaat taagataacc gcccatggaa
     6121 gcaatgccca aacataaggc cagctcgcac cccagattga tcccgctaac catgcattca
     6181 caaatccata attttcctta gataattgga tcgtgcatag caatgttaat gcagaaaggc
     6241 cggcattaac ggcaataccg gacaataaga ttcgattcat acccaaaaag ccatttcgat
     6301 cataggccgc aagatagaca cagcaagcag ctaaaacacc acctagaaag gctacaacta
     6361 gcaatagaaa cggcagcgaa tagtgcgaaa agaaacctaa ataaagcatc actaaaaatc
     6421 ctgctccggc attaatgccc aatatactag aatctgccaa ttcatttctt gttactcctt
     6481 gcaagatata accagaaacg ccaagacaag ctcctgctaa taaactgatc agtaatcgag
     6541 gaactcgaaa ttctctaaca atcagcgcca aagaagatga ttctttccct atgaacgtgg
     6601 caaaaagctg atccaggtaa ataaaaggcg taccaatcat taaactacct atagacagga
     6661 tgacaagtac aaaaagcaag aaatagaacg tccaatatat ttttttcacc gttgttccct
     6721 ccttacttgt atcagtaaaa acggaacgcc aatcaatgca atgactaatc caaaaggtgt
     6781 ttcaaaaggg ggattgatca tcctcgctgt aaaatccgcc aacataacca atccaccgcc
     6841 aactaaaaaa gagacaggta gaacataacg atagtccgtt ccaaccattc cgcgaacaac
     6901 tggcggaacc aacagcccaa taaatgaaac aggtccgact aacgaaacgg caatggctgc
     6961 taacactgca actagacaga aaacaatcaa ccgcgttcgt ttaggatgac ttccaagagc
     7021 taatgcatgt gtttcactca aatgaatcaa agatattgct tttcctaatg aaaaagaaac
     7081 caaaacagca agtatatata aaggaaacaa tactgctaat tgtttccatg tgatattggc
     7141 agctccgcct acaaaccaaa aagcaagatc ctgctgcaga ttaaagaatt gggtaagtgc
     7201 ttggctgata gcagtaaaaa aagaactaat agctacgcca gctaaaatca gctgtaaagg
     7261 attcatgccc attttcgaaa actgcaaaaa accaaatacg agcaaagtta cagcaaaaga
     7321 acctgccaat gacattagaa acacagaaaa atcagaagct ttaggccaaa aagcaaaact
     7381 aagggccatt gccaacgaag cacccgcgtt gattcctagt aagcctgaat ctgctaaagg
     7441 atttcttgtc actccttgca tcaatgcacc gctcaatgca aaacaactcc ctacaagaaa
     7501 tgctcccgcc acacgaggca aacgaatatt tcgtatgatt tggtgacttt gttttgatct
     7561 atcaaaatca aacaacgcct gccaaaaatc tgcttttccc acagtcgctg cgccatttga
     7621 aagagcaaca aaaaaaataa tcaaaagaac aaaacagacc aaaagcagga gtctattttt
     7681 cttcatagtc ctcataattg atcctcctgc tcttttggca acaaatcata ggttagaaaa
     7741 actggtttat ttgttcttgg aagcgtgaca atcgttgcat caatctcaaa tatttttttc
     7801 atacactctt ttgtgatcac ttcttcaggt gtaccttctg tgatcaactt accgtttttc
     7861 attgctaata aataatctga aaaacgtgaa gcatgattaa tgtcatgaat cgacatcaag
     7921 atcgttgttt gtttcatttg attgatttca gtcaataact gtaggatctc taattggtgt
     7981 gccggatcta aaaatgttgt cggttcatcc aagatcaaga tatctgtttc ctgagcaagc
     8041 gccatagcga tccagacacg ctgggtttgt ccaccagaca gcgttgcgat atcccgcata
     8101 cgaaatccag ctagacctgt agcatccaac gcccactcga ttgcttcatg atcttgcgtg
     8161 tccatgccac tgaacagttt ttgataaggg tatcgtccgt aagatactac ttcttctact
     8221 gtcatacctg tagggtgttc actagtttgt gctaataatg taattttttt tgcaagttct
     8281 tttgaactgt atgtagagat atcagagtta ttgacaatga tctttcctga tttcacaggt
     8341 agtatacggg taaaactttt caataaagta gattttccac ttccatttgg cccaatcaaa
     8401 ctagtgattt tcccttcagg aatcgtaaca gacatgtttt caatgatttt tttgttttcg
     8461 tagccggctg caatgtcagc tgctcttaat ctcgtcattt cacccatcct ttcgctgaaa
     8521 atcattctca attatgacgc tgtttcaaca tttcgtcaag cgctactttc atatctatag
     8581 ccaaatgaag cggttgacag cgttcgaaat atcaggaata ataaccaatg agaaagattc
     8641 tcaattatgt taggaggaaa gaaatgaaat taaaaacaaa agtattcaca ttatctagca
     8701 tattgctgct ggctggagta ctgagcgctt gctccccctc ttcagataaa gaaacagcta
     8761 caactgccaa ttcgacgact tcatccaata gtactcatac agtcacagat acacttggac
     8821 ataaagtaga gattccaaac gcgcccaaac ggatcatcgg cagttactta gaagactatc
     8881 tgattgcact aggggaaaaa cctatcgccc agtggactgt tggcagtggt tccattcaag
     8941 attatctgca agatgattta aaggatgtcc caacgatttc ttatgacctt ccttatgaga
     9001 aagtgttaag ctttgagcct gatttgctgt tgatttcttc ttccgctact gtcgaaggcg
     9061 ggaaatacga acaatatagc aaaatcgcgc caacttatgt tgtaaaaaac ggaacagatg
     9121 tcacatggga agaacaacta aaagatgtcg gcaaagcttt ggcaaaagaa aaagaggcag
     9181 aaaaaatcat ttccgattat caaaaatcca tcaagaaaac tcgagaagaa ctagcagata
     9241 aaatcgagaa caagacagct gcagtgttgt gggtgaccaa caactcagcc tttatggtca
     9301 gcaaaaatcg gtcaagtgga agaatcgtat acgacgatct ggaatttggt gttcctgatc
     9361 tagtggaaga ggtctccaaa aaagccactt ctgactggtc tgctgtttcc tctgaaaagc
     9421 tagctgaatt agatgctgac tacatcatcc ttgtgaacag tgacaaagat gctgccatgt
     9481 tcaatgaacc aagctggaag aatcttaaag cagtgacaga caatcatgta ctgcaattcg
     9541 gacctgaatc tagttggcta tataacggac ctattgccag tcaggaaatg gtcaatgata
     9601 tcaaagaaaa actaaattga ttttaactaa aaaaagaatc ttcacgatta gtcgtgagat
     9661 gctttttttc ttattaacaa actagacgca tagattttat tcttcttttg aagcaaccct
     9721 attttttact ctttcccatt tttgaaaacg atgggcatat agattttctt gatccgttat
     9781 cccctcatac tcctctataa gggcgaagtc tgaaaaatca atattcccaa tatacgtatc
     9841 gccttcaaac gtgctatcga tcaatgttcg ccagatccga ccagtgtaag gaagaagttc
     9901 ttgaaaaatc cgactaccac ccgaaacata gatatcttct gagatcgttt gactatacga
     9961 caaaagttca tcgatcgaat gcattacttc cgcattttct ttcgtgcgaa aatttggttg
    10021 cgttgtcaac acgatgatat ggcgataagg aagtgaaaga tcccccattc cttcataggt
    10081 ctttcttccc atcactaaaa ttcgatccat tgtatgttcg cgaaagaaac gcatatcatt
    10141 tggcaatcgc catggtaaca aaccattttt gccgatcaga ccatttttat cttgtgccca
    10201 catggaaata aacatattat ccgctcctta tgattttaga tgttttgaga taaaaaaagt
    10261 ctcaattctt tgaccagcaa ggaattgaga actttaaaaa cattattttg tttcacggtg
    10321 caaagtaact ttgcgttcgc gtgggcaata tttttgtttt tccaaacgat caggattgtt
    10381 acgtttgttt ttgcttgtta gatagttacg ttctttacaa gaagtacact ctaaagtgat
    10441 gtttacgcgc atgtttttcc ctcccatttt tacaagaatc ctctaagatg actctcagac
    10501 ttgattatct taccatgatt ttttctagat tgctagtgtt tttcacgatt ttgttaagta
    10561 aaactacttg acgataaata caacggtttt acttctcaaa caaaatcttt ttctttttgt
    10621 ataaataccc ttacacttgt ttatatagat tatgtttttt aataaagggg gatactaata
    10681 tgccatggaa catgaaggac tttcctgcat caatgaaaaa cttagataaa ttgacaagaa
    10741 aaaaagcaat cgacatcgca aatgcattat tagatgaagg gtaccctgat aaccgtgcga
    10801 tcccaattgc cattgatcaa gcaaaaaaat ggtatgaaga tgctagtgaa tcagaaaaac
    10861 gaactttcga aaaagaaaaa aatccaagca agacggatga acacgatacg aatcctcgtg
    10921 ctggaaaact gcttgattca gatgtgatcg tagaatatga ggaagaacaa tggatcgtga
    10981 aatctaaagg ggcaaaaaag gcaagcaacc attttgatac aaaaaaagaa gcaatcgaaa
    11041 aaggaaagca agtagcacaa aacaaagaat caactttgtt catttataaa aaagatggga
    11101 caaaagaaaa agaaatcgat tattgaacat atgtaaaggg gttgtgacga aaatcgtgtc
    11161 acaaccccct tatgttcgaa acaatggtgt tcctctgcca actccgaacc ataagtcata
    11221 atatctataa aacgggaaga gctgtttttt gaaattgttt cttatcactc agtgttaaac
    11281 ggtaggagaa aacctctttt ttattgatat ttgattttca tcaaacagtc acatagtcga
    11341 ttgcttctgc ttgcggaatc gtttcatcta caaagtagac tttataccat gtcaagaaac
    11401 tgaagattgt ccagattttt ctttgctctt ctttacgtcc ttcgtgatgg tcatctagca
    11461 acttcatgat tttttcttga tcaaaaaatt cttttgcaaa atctgcttcg aataatgctt
    11521 tgacttgttc gtagccgtgt tcttctttca accaagcttt gattggcaca gggaatccta
    11581 atttcacacg attcgaccat tcttccggaa gatgtttgtt cgcagcttgt ctgaaaatat
    11641 cttttgtgtt gttttgattc aataaaaact tacttggtat ttgttgagcc aatttcatga
    11701 cttcgatatc caataaaggt actcggactt ccaaagaact tgccatagaa agtttatctg
    11761 cctttaacaa gatatctttt ggcatccatt gatgaagatc gacatattgc atcttattga
    11821 cttcatcttt gattccttct gttttctcgt aatgaacagt catgatttca tccacagatg
    11881 gtgctgtttg atattttggt gttaacactt ctgatgcttc tgattcttcg aacactcgag
    11941 catgaccgat aaaatattct tttgcaggag caagtgattc atataaatgg atccgtccat
    12001 ggaaattttt catttttccg attttacgtc ctaaattata gcgtgttcct tttggtaatt
    12061 ttttcaatcc ttgagcaatc acacggatga attttgaatt cgtatggaat ccatacgttt
    12121 ggtatcccgc aaacagttca tccgcccctt ctccagattg gactacccgc acactttgtg
    12181 aagcaagatt tgctaaaaag tacaaaggta cgcaagaagg atttgaatca ggttcatcta
    12241 agtgatactg gatcaaaggg aatgctttga acgacatgtc tccgtcgatc actgctgctg
    12301 tattatctaa gtccaatagt tcggttagtt ttctggcttc aatcgcttca ttataggttt
    12361 tatcatcaaa cccgattgag aaagaatgat ctggtttcaa tacagaggtt acataactgg
    12421 aatcaacccc gctggataag aaagaaccaa cttctacatc actgaccgta tgtgcttcaa
    12481 tagaagcttg aacggtttcg tcgatttttt cgatccattc ttgtcgggaa tgcgtttctt
    12541 ttgtttcaaa atctgcatcc caatattgct gaatgtctag ttttccatct ttatacgtat
    12601 aataatggcc ttcaggcaaa cgatagacat ctttgaagaa tgtttcgccg tttaatggag
    12661 aatattggaa agtcatataa ggtttcaacg cttctttgtt caattctttg ttaaaatcag
    12721 gatgaggcag aaaacttttg atttctgaac caaacataaa tgttccgttc atttctgcat
    12781 aataatatgg tttgatccca aagtggtctc ttgcaccgaa taactcattc ttttcattat
    12841 cccaaatagc aaaagcaaac attccgcgga tcttttgcaa taattctgtt ccccattctt
    12901 cgtaaccatg caaaaggact tctgtatctg catgtgtttg aaaaacgtgg ccagctgcaa
    12961 tcaattcttc tctcaaaggc tggtaattat agatttcgcc attaaaaata atgatctttg
    13021 tccgatcttc attgtaaata ggctgagtac cgccctctaa gtcaataata cttaatcttc
    13081 tgaatcctaa agcaacatgt ttatcgatat attctcctga actattaggt ccacggtgaa
    13141 cgattcgatc catcattgta ttaataattg tttttttgtt gtccttgtcg ttaacaaaac
    13201 ctacaatacc gcacatttaa ctttcacatc ctaatataaa attactgttt atttcattca
    13261 tatgcatctt atcatagaat aaatagcgga cgaatatttt ctctcttaca atttcgtcac
    13321 gtgaaaacaa aagatgaaag tttctgtttt tacagaaaat aaagtcccaa tatctaattt
    13381 accatgtttt cttttttgag gctaccttat tctcttcgtg ttaaaaaaat attagtggat
    13441 gctatttcca aaa
//
LOCUS       DAZUMK010000070        13149 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000070 DAZUMK010000000
VERSION     DAZUMK010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.072"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(78..150)
                     /locus_tag="ACG808_001869"
     tRNA            complement(78..150)
                     /locus_tag="ACG808_001869"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(115..117),aa:Lys,seq:ctt)
     gene            531..1100
                     /locus_tag="ACG808_001870"
     CDS             531..1100
                     /locus_tag="ACG808_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFS5047493.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1087..1947
                     /locus_tag="ACG808_001871"
     CDS             1087..1947
                     /locus_tag="ACG808_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS5047494.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2137..2841
                     /gene="yycF"
                     /locus_tag="ACG808_001872"
     CDS             2137..2841
                     /gene="yycF"
                     /locus_tag="ACG808_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFS5047495.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2846..4681
                     /gene="walK"
                     /locus_tag="ACG808_001873"
     CDS             2846..4681
                     /gene="walK"
                     /locus_tag="ACG808_001873"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFS5047496.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4678..5994
                     /gene="yycH"
                     /locus_tag="ACG808_001874"
     CDS             4678..5994
                     /gene="yycH"
                     /locus_tag="ACG808_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFS5047497.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5995..6864
                     /locus_tag="ACG808_001875"
     CDS             5995..6864
                     /locus_tag="ACG808_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFS5047498.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6919..7728
                     /locus_tag="ACG808_001876"
     CDS             6919..7728
                     /locus_tag="ACG808_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS5047499.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7831..8460)
                     /locus_tag="ACG808_001877"
     CDS             complement(7831..8460)
                     /locus_tag="ACG808_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS5047500.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8630..9924
                     /locus_tag="ACG808_001878"
                     /pseudo
     CDS             8630..9924
                     /locus_tag="ACG808_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
     gene            complement(10000..10662)
                     /locus_tag="ACG808_001879"
     CDS             complement(10000..10662)
                     /locus_tag="ACG808_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFS5047501.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10887..11171
                     /gene="groES"
                     /locus_tag="ACG808_001880"
     CDS             10887..11171
                     /gene="groES"
                     /locus_tag="ACG808_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFS5047502.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11222..12847
                     /gene="groL"
                     /locus_tag="ACG808_001881"
     CDS             11222..12847
                     /gene="groL"
                     /locus_tag="ACG808_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFS5047503.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4371 a         2265 c         2833 g         3680 t
ORIGIN      
        1 ataaaaaaag aacagcgtaa atgttttcga aatgctttcg caccaacatt tacctgttcc
       61 gtgggtttgg cacccaatgg gccgtgaggg gctcgaaccc gcgacccgct gattaagagt
      121 cagctgctct accaactgag ctaacggccc gtacaaaaag tacttaataa tagtagcact
      181 aagaatttta aaatgcaaga ctacatttaa agaaaatcag aaaaatcttt acttccttat
      241 aggaaatcat tcatagatta ttcacatttg ctcgctatta tataagcata ccaacaaaca
      301 aagttcttgc aagagaacaa accctaacac ttttttcatt ttttactcct ttcccgccgt
      361 ctccccggcg ggtctcctgt tcttaacgat aaaagacaag aaggcttagc cttcttgttt
      421 tttgttttca tggaatcccg ctaatcaaat cacctagtta aagcaactag attgaatgtt
      481 tatcataagt ctgatatcat gtttatgaaa tgaaaaaagg agcggttatg atggaaaaaa
      541 tgcgtcagca attaatggag tgggggaagg aactagaaac attccgtctg cctcactggg
      601 aagaactccc tgacttagaa ctttatatgg accaagtcag aacgttagtt gatcgttatc
      661 tttcacctgt gatacaagga gaaaagcatc cattgcttac ctcttctatg gtaaacaatt
      721 acgttaaact aggtttgata ccagcaccag ttaaaaaaag atataacaaa gaacacgtag
      781 catttttact tgcaatcact acgttgaaac aagtattgac tattcctgaa atcaaagaag
      841 ggatattgtt ccaaggaaaa acagtaggaa ttcgtgaagc ctataatcta ttttgcgatg
      901 aacaagaagc agctgtgtgg atggtcagcc aattagcaca aggtaaatct catccgcaaa
      961 aattcgacca ggcaacccct gtagaatata ttgctgtaag agcggcaact ttatcgtttg
     1021 caatgaaact tcttgcggaa aaaacgattg ttctagaaac cgaatattta aaagaggaga
     1081 aaacaaatga aaaacaataa aatcgcaatt ttagtagatt caggtacgga tgtgccagaa
     1141 gaattgataa aaaaatacga gatgtatatg atcccattga aaattattta taaagatcag
     1201 gtatatacag ataaaattga cattacctct gaagagatct atcaacgttt gccagaggaa
     1261 atccctggta catctttgcc ggatggtgag tcgatcacag aaatctttga gcaaatcaaa
     1321 aaagacggtt atgaaaaggt acttgcggta acaatttcca gtggattgag cgggacatat
     1381 aatgtggttc gtttaattgc acaacagcaa gaagaattgg aagcatatgt actagatacg
     1441 aaaaatattg gtattggtgc aggtttttct gcgatccaag cagcaaaatg gctggaagaa
     1501 ggagtagaat gggatcaact gatcagtaat ctgaatgagt tagtcgaaag aacaaaagtt
     1561 ttctttaatg ttgcgacatt ggaatatctt caaaaaggtg gacggatcgg tttagttgct
     1621 tctattgtag gaaccgcttt aaagcttaac ccaatcatct catgtaatga agaagggatt
     1681 tattacacag taggaaaagc taggggacgt aaaaaaagtt tggataaaac ggttgcttac
     1741 gtcaaagaaa aaatcggtaa tgcaaaggtc ttcaacttag ctgttgccca tggggatgcg
     1801 aaagaagaag caattgaaat ggaagcgaga cttaaaagag agttcccaca agcaaaccaa
     1861 atctactttg gccagatttc tccggcatta gtagtccaca ctggtccggg attattgggt
     1921 gtcggtgtac aagttttaga aggataaatc aaaaaaagga aacactgagg tgaaaatcag
     1981 tgttttcctt ttttattgaa gttagaacaa aaaataataa agtattttgc actttcattt
     2041 taattttctc ttttgtttta taggcaaaga tgaaacaaaa gcagaaatac ggtataatga
     2101 gaaggtatga caataatata taagaggtgg ctctttatga aaaaagtttt agttgtcgat
     2161 gatgagaagc cgatttcgga tatcgtcaaa ttcaatttag ccaaagaagg ctatgatgtt
     2221 tatactgcat atgatgggga agaagccttg gaaaaagttg ctgaggtaga acctgatttg
     2281 attttacttg atttgatgct acctaaaatg gacggcttag aagttgctcg tgaggtacgc
     2341 aaaacttatg atatgccgat cattatggta acagccaaag attctgaaat tgataaagtc
     2401 ctaggcttag agttaggcgc cgatgattac gtgaccaagc cattttccaa ccgagaatta
     2461 gttgcacgtg tcaaagcaaa tcttcgtcgc ggtgccacgg ctgcgaagga accagaagag
     2521 gcagcacctg cggaattgac gattggtgat ttgacgatcc atccagaagc ttacatggtg
     2581 acgaaacgag gagaaacaat cgagttaaca catcgtgaat tcgaactact cttttacttg
     2641 gcgaagcacc ttggacaagt gatgacaaga gaacatttat tacaaacggt ttggggttat
     2701 gattattttg gagatgtacg aacagtggat gtaactgtac gtcgtttgcg tgaaaagatc
     2761 gaagataatc caagccatcc aaattattta gtgacacgtc gtggtgtcgg ttactatttg
     2821 cgtaacccag aacaggagta attggatgaa aagaaaagtc cgcttttttc gatcagtcaa
     2881 ttttaagatc gctattacct ttattctgat tttattgatc tcgatcgaaa ttattggtgc
     2941 ctactttata cgtggtttag aacgttccac gatcaataca tttattaaag atatgaatca
     3001 aacggtggaa tcattagcaa caacaatcag cccggaattg aatcgaaaag ataatgcaga
     3061 tgatgaagag gtaaatgcga atatcaaaag atttattgaa aacagtgcga ctagcgatat
     3121 catcgaaata cgggtcgttg acgaaaaagg gatcatccga ggaacaaccg atgtcaatga
     3181 acaaagcgct gtgggaaaga aaaatgatta tgttgatatc aatgatttca cgactaaacg
     3241 ttatgttgct ttagataacg acgataaacg ggtgaatatc aatgttcaac cgatcctgtc
     3301 cccgaccggc gatgccgtta tcggggctct ttacgtcaaa agtaatatcg aacaaaaata
     3361 ttcggaaatc aataacacag cagtcatttt ctttactgcc tcactaattg cggcatttat
     3421 ttcaatgatc gtatctgttt tagttgctcg ttcgattaca cagccgatcg gtgaaatgag
     3481 agaacaagcc attcgaatcg ctagaggaga ctacagtcgg aaagtcaaag tgcatggaca
     3541 agatgaactt ggacagctgg ctgatacctt caatcagtta gctgaacgta ttgaagaagc
     3601 tcaagataca atggaagctg aacgcaatcg tttggatagc gtgctatccc atatgacaga
     3661 cggcgtcatt gcgacagatc gcaggggtaa agtcattacg atcaatgata tggctgtttc
     3721 cttgctagat atcaaaaacg aggaagcaat cggacaatcg attttgacac ttttagatat
     3781 cgaagaagaa tatacgttgc ggaaattatt agaagatcca aacgaaatgg tacttgaccg
     3841 ttcaaccagt acattggaaa gtgatcagat gatcttgcga gtagactttt ctatgatccg
     3901 tcgcgaatca ggttttatca gcggattagt agctgtcttg catgatgtga cagaacaaga
     3961 aaaaacagaa cgggaacgtc gtgaattcgt ctctaatgtt tcccatgaac ttcgtacacc
     4021 gttgacaagt atgcgtagtt atatcgaagc attgagcgaa ggtgcgtgga aagacgagga
     4081 agtagcacca aattttttga aggtaacgct agatgaaaca gaccgaatga ttcgaatgat
     4141 caatgacctc ttaaatctat ctcgcatgga tacaggtaat acccagcttc aattagaata
     4201 tgtcaatttc aacgagttag tgaattttgt tcttgaccgg tttgatatga tggtgggaaa
     4261 tcaggaaaaa aattataaga tccgtcgtga atttacgcaa agagatcttt gggtcgaagt
     4321 agatacagat aaaatcatcc aagtagtcga taatatcatg aataacgcga tcaagtattc
     4381 accagatggg ggaacgatta cttgtcgctt attagaaacg cataataacg tggttttaag
     4441 tatcaccgat caaggattgg ggattcctaa aaaagatctg aaccgagtgt tcgagcggtt
     4501 ctatcgagta gataaagcac gtgcaagagc acaaggtggg acaggacttg gattagctat
     4561 ttctagagaa gtgattaagg cgcatcgagg cgctatttgg gcagagagca aggaaggaaa
     4621 agggtcgact ttttatatct cgctgccata tgaaccttat gaggaggaat ggtgggaatg
     4681 agaatctctg aaaaagtcgt tcgtatagga ctcattttga tggttgcgtt aagcatatac
     4741 ttctcttatg caatctggct gagcccagca ggaaaaagtt caatcaacgt agatgagaca
     4801 aacactcaaa tgatcgagtc tcaaagcaat cgaaaaactt ccgaagtctt tcttccgctt
     4861 catgttacat ggcaccattc gggaatgatc caagaaacca acagtgaaaa tctcgtgtac
     4921 cgattgcaat tgatcatcga taaagcaaga tttggaaagc tttctgaagt agtttcagga
     4981 gacaaatcac agtttgaaca ggtgaaaaat ctgaactctg gttttgagtt ggcctataat
     5041 gcgccttttt tgctaagaga atacaaggaa gcatttgatt tagatttaga tgtgtcgaac
     5101 ttggcaaaag aaagcgaaaa tatgtatttt actagaatac aattcgatca aggaaataaa
     5161 aaaatccgat ttttgaatta ttcgaaccat ctcgtatatg aagcaaatat ttcgttgaat
     5221 tggacagata tcaataaaga attaacagca aaagacacaa aatggacaga aatgacaaca
     5281 gaaccatcga cgatcgacac gcaatatgct acgaaacatt cgatcaaact gaaaaagtat
     5341 agttatatcc tgtcacagca accctataca ctttttagaa atgctttttt ccagaaacca
     5401 gatagcgtga aaaataatga tgaaagcaat gaattgattt tttacggtgg gaatgaaaca
     5461 atgtctatcc attcggaaac gcaagtagtt gattttcgtg gagagctgcc agagaaaaaa
     5521 gaaaatgcta gtgtattttc tgaaagtttc ccctatatta gcaaattagg cagttcttta
     5581 gggaacctca gatattttga ccgcagtgac aatacgatcg attatcggat attcgtggaa
     5641 ggcttcccgg tttttgggac agacagcaga ggacagattg gtgtggaagt tggcggtgaa
     5701 acgaatggtt ctgtgcaagt gaatatccaa accagtttga atacgattca agtgccgata
     5761 ccttcagacg aagaagtaga acttgcaagt acgaatgaaa tcatccagca gttaaaagct
     5821 aagggagcag atagcaacaa gattggttca gtgatcgttg gctatacatg gcaaaatgtc
     5881 aaagaaacga atcgtgtcgt tgatctctta cctgaatggt atgtgaaata taaagataac
     5941 tggtattcag ctaacgacct tctggaaaaa ttacctggat tggaggcgaa ttaattggat
     6001 ttcaaaaaaa tcgaatggat atttttcgtc gcttttttag ggttgaatgt gtttttattc
     6061 agcagttatc atgacgcggt ataccaagaa aacaatgtca ttcgctctaa tcagactgaa
     6121 tcaatccaac aacgtctggc tagcgacgat atcacttaca cggggaagat ttcgtctgag
     6181 aacaagcaag gctattatct tagcgcggag cagaccaatc ttagcacttc attagcgaat
     6241 tatcgcaagg ctggtaaaag cggcttgctg gcaagtggtg tgtttttaga cggtgatgtt
     6301 ttgacgaaat ctttatccag ttcagatgct gaaaagaacg tgatcgatcc agataaaatt
     6361 agtagttcct tggaagattt tttatctaac aaagacaata ttttattcgg taatgattat
     6421 gtgtacacga aaaacttttc tcatttagaa gaagaaccac tagaaatcca agcaagccaa
     6481 gagtatgaag gcatcccatt caatgatgat acagcaaaaa tcgttctttc tttagaaaaa
     6541 aacgataatg attatagcat cacgaaatac atgcagacgc atttatctga tatcgagcag
     6601 ctgcgcgaaa agacggaact gtatacggaa gaggaagcaa tcaatacatt gtacatcaat
     6661 aacaaaatac caagaggatc aaaaatttta tggcgtcagc tagcttattc ctgtattctg
     6721 aaagtccggg aaaaaaatgt gtatgttcct gtttggcatg tagcgatcga aacaagcgat
     6781 aaagtggtcc aaattgagag tgtcaacgct ttcagcaata cgatcgtgac aaacaatact
     6841 gtaccaaaag tggaagatca ataaaaaagc gagtataatg aaaaaagttt acaaagtaag
     6901 aagggaagta ggagtcaaat ggctgaagaa aaaccagctt ttaaaatcag tatcctagca
     6961 agtggaagtt cgggtaattc actttatatc gaaagtgaaa aaaaacggct tttggtcgat
     7021 gcaggactta gcggtaaaaa aatcacttcc ttgatggctc aaattgatcg gacaccggac
     7081 caattagatg gaatacttgt gacgcatgaa catcgagatc atatacatgg cgtgggtgtc
     7141 ttggctcgta agtatcattt ggatgttttt gccaatcagc agacttggga agcgatggaa
     7201 ccactgattg gaaatgtacc agtggaacaa aaacatctat ttgagatggg gaaagtcaga
     7261 acattcggcg atatcgatat tgagagtttc ggcgtctccc atgacgcagc ggctccgcaa
     7321 ttttatcgtt tccataaaga aaatcggtca ttcgtcatgt tgacggacac cggatattgc
     7381 agtgattata tgcgaggtgt cattgaaaat gccgatggtt atttgatgga aagtaatcat
     7441 gatcttgaga tgttacgcat gggaccttat ccgtggaatt taaaacagcg tattttagga
     7501 gatagaggac atctttccaa cgaagacggt gcacttgtca tgactgatgt gatcggtaat
     7561 cggaccaaac gaatctatct tggccatctt agcaaggaaa acaatatgaa agaacttgct
     7621 catttaacaa tggaaaatgt tttgaaagaa aaagattttg gcgttggaca tgattttgaa
     7681 atctatgata cagatccaga tgaagcaact gagctatttt cgatttaaac agttcaatca
     7741 ataaaaatat aaaaagcaga agataaaatc aatcagggtt ggttttatct tctattttta
     7801 tttttttagc atattaaaaa aataaccgta ttatcgactg actacactga tgtgctgctt
     7861 ttcatgtttc tcatccagta tctcatctac taatgcaatc gcgtaatctg ccatgctgat
     7921 cgtactgtcg ccagcagcgt ttctttgtaa ataatcatca cttaacaaat aagtacctgt
     7981 acgtttgcca tgtggatcaa ataaagaagc tggactgata taagtccaat gtagattttt
     8041 agattgtttc aattttaaca atccttcgta catattgaaa gctgttggat agaagggtgc
     8101 atttttaggt acttggtcaa tcatacgagt cgtcttttct gtatctaaga ataacgaaga
     8161 ggcaccccca acgacaatta agcgtgtatc ggttccttca agcatctcaa tcaaatggga
     8221 caaagaagtt tgatgcattt cttcttttcc tttgggagcg ttgaaagcat caacgacaac
     8281 atcaaaagat gcaagatcag tagttgtcaa ctcaaataaa tccttttgta taaacggaac
     8341 atctgatggc aatttttctg gatgacgcac gattgccgtc acgtctaaat ctctggacaa
     8401 tgcttcgtct aaaataaatg aacctgcatg gccagtagct ccaatgagtg caattttcat
     8461 aaaaaacctc cttttttatt cctgtccatt gtaagcagtt gctgaatatt cgtcaaagaa
     8521 tccgattttt gaaaagctca gtgcgcattc ctttcatggt tcgtgatcat tgggtatagt
     8581 taaattaaga aaactgattg ataaaaatag aagttgaagg agataagaaa tgaaagagtg
     8641 gttagatcgt gcctttttac caatgataac atctgttgtg aatcttagac ccttaactgc
     8701 tttgcgtaat ggaatgactt acacgatccc aataattatt gttggctcgc tgtttttctt
     8761 atttgctcat tttcctgtgg attacatcgc tcagtggata gacaaaacag gattttctat
     8821 gtattttaca caagtatacc aggctacatt tgatgtgata gccttttgga tcgtttttgc
     8881 cattgcatac gcttatgcaa aagaagaaga catcgatcgc ttaccagtcg gcataacagc
     8941 cttgtctgct tatctactgc ttattcagcc actaaatact cagatagata ctgtctggac
     9001 aggtgctgaa gggatactag gagcagttac aactgggctc atcatcggat ggggctatat
     9061 gcacgtttag taagacttgc gaaagggtgg aagcaaagag agaagatacc tgaaaatgtt
     9121 tggcagtctt ttacttcatt gattccaatc attgttatag tcacttttac gcttatcggt
     9181 tcagcggttt ttcaacagtt gttcagaatg actgttgttc aaggagtatg gacatatgtc
     9241 cagcttccca ttcaatcgtt catagacacg ttgtttggtg tgcttttatt aggttttttt
     9301 gttccgtttt tatggttatt tggtcttcat ggatctgcga tggtcaatgg gttggttagt
     9361 cccgtcttgc aagcgaattc attagcgaat gcagaaatct tagcctcagg acaagagttg
     9421 acggtggcaa atggcggaca tatcgttact cagcaatttc tcgatcagtt catgacggtt
     9481 acgggtgctg gattgacgtt aggggctgta ttttttatga cggtttttgc gaaatcgaag
     9541 aaatacagag aattaggaaa tcttgctctt ttaccagcct ttttcaacat caatgaatca
     9601 attatttttt ccacaccgat cgtgatgaac ccgatgatgg ctgttccttt tattttcgca
     9661 ccggtcgttt caggattgat tacctattcc gctttatatt ttggattcgt tcctttattt
     9721 acagcagtcc aagtcccttg gacaacaccg ccgatcctct cgggtttctt agtaggcgga
     9781 gtgcctgcag cgattttaca aggtatcgtg ttgtcaatta gttttttcat ttatttccca
     9841 tttttgaaaa aaatagattc tgcaaactgc aaaaaggaaa gacagacaaa aaatgatgga
     9901 gcagcagaaa agatgaacga ctaaaaaaca aaaatactcc tgtcgcttta gattgggaaa
     9961 atcaccaatc taggatgaca ggagtattta tagtcatgtt tatactagcc ctttttggac
    10021 agctaattga acaagaacaa ctaatgtgtt cattcctacg tgtgtgatca tcgatgtcca
    10081 gatccgacca gttgctttgt actctaggct gaagaagaaa cctaggaaaa agtagattag
    10141 cagatgacca tcattatgag caaacgcaaa cagcaaagag ctgatcactg ctccgaccca
    10201 aaaatttgaa tatcgactga tgatccccag tatactgcgg cggaatacga actcttccat
    10261 gatgggtcca gcaacagttg tcgccaaaat aaaagcgggt gcttccatga tcatctggat
    10321 gatattttgt gtattctcgg aaggggttgc tttgccaaac aatacagatt ctaacatgac
    10381 agcgatgctt tgcagaatca atgcgacaaa gattccaacc aatccataaa taacaatctt
    10441 tggagcagaa gcttttttta ccgctgattc gatcgctaag ggttcacgtt gcttaaaata
    10501 aaaaatagct aggacaacgg cgcctagtat atacatgact gcagttgctg tgatcaattc
    10561 agacgtagat tttacaagtc ctatcgaagc taggatcaag ggtgagataa aaattagacc
    10621 atagcaaaaa atgctgagga agctatattt ttttaaagac atgtaattcc tccttttaag
    10681 ggataacaag catgcaaggc aagcttatat cgattgattt caattatatc ataatttcaa
    10741 taagcagctg tcagaataaa atttcttttc tttgatttaa aagcttgcaa aaaagaggga
    10801 gaatgagtat tattaaaaat gtaggttagc actcgaaggt taagagtgct aacagattaa
    10861 aaaaagagtt atcggaggga tttatcgtgt taaaaccatt aggtgatcgc gtcattatcg
    10921 aagtcgcaaa agaagaagaa aaaactgtcg gaggaatcgt tttagcttct gctgctaaag
    10981 agaaacccca aactgggaca gttgttgcag tcggtgaagg ccgtttgtta gaaaacggtg
    11041 aaaaagttcc tgccgctgta aaagcaggcg accaagttat gttcgaaaaa tacgccggta
    11101 cagaagtaaa atacgaagga aaagaatatc tgatcgtcgc aggaaaagat atcatggcga
    11161 ttgttgagta aatttggaag ataaaaaaat aatcaaaaat aatcaatgag gtgaatcaat
    11221 catggcaaaa gaattgaaat ttgcagaaga cgcacgcgca gctatgttac gcggggtaga
    11281 taaattagca gatacagtga aagtaacatt aggacctaaa ggccgaaatg tcgtactaga
    11341 aaaatcttac ggctctccat tgatcacaaa tgatggtgtg acgatcgcaa aagaaatcga
    11401 attagaagat cattttgaaa acatgggtgc aaaacttgta tcagaagtag catcaaaaac
    11461 aaatgatatt gcaggagacg gtacaacgac agcaactgtt ctgacacaag caatcgttcg
    11521 cgaaggttta aaaaacgtaa ctgcaggtgc taatcctcta ggcattcgtc gcgggatcga
    11581 attggcaaca aaagcagcag tcgaagaatt gcataatatc tcaactgttg ttgattcaaa
    11641 agaagccatt gcacaagtag cagctgtttc ttcgggttct gataaagttg gacatttgat
    11701 tgctgatgca atggaaaaag ttggaaacga cggtgtcatc acaatcgaag aatcaaaagg
    11761 gatcgaaaca gaactagatg ttgtagaagg tatgcagttt gatcgtggtt acttatcaca
    11821 atatatggta acagataacg acaaaatgga agctgttcta gaaaacccat atatcttgat
    11881 cacagacaaa aaaatctcta acatccaaga tattctgcca ttgttagaac aaatcttgca
    11941 acaatcacgt ccattgttga ttattgctga cgatgtagat ggtgaagcat tgccaactct
    12001 tgtattgaac aaaatccgtg gaacattcaa cgtagtagct gtaaaagctc ctggtttcgg
    12061 cgatcgtcgt aaagcaatgc ttgaagatat cgctattttg acaggtggta cagtaattac
    12121 tgacgatcta ggtttagaat tgaaagatgt aacaatcgaa aacttaggaa atgcttctaa
    12181 agttgttgta gataaagaca atacaacgat cgtagaaggt tctggagaaa aagaagcaat
    12241 cgaagctcgt gtgcaattga tcaaaaacca aattgctgaa acaacttctg attttgaccg
    12301 cgaaaaatta caagaacgct tagctaaatt agcaggtggt gtagctgttg ttaaagttgg
    12361 tgcagctact gaaacagaat tgaaagaatt aaaattacgc attgaagatg cattgaatgc
    12421 tacacgtgcc gcagtagaag aaggaatggt ctcaggtggt ggtacagctc tagtgaacgt
    12481 tatcagcaaa gtttctgctg tagaagcaga aggagacgtg gcaacaggta tcaagatcgt
    12541 tgttcgtgct ttagaagaac caattcgtca aatcgctgaa aacgctggtt atgaaggatc
    12601 agtgattgtt gataaattga agaacgtgga actaggcact ggtttcaatg ctgctactgg
    12661 agaatgggta aacatggttg aagcaggtat cgtagaccca actaaagtaa cacgttctgc
    12721 cttgcaaaat gccgcttccg tttctgcctt gttattaacg acagaagcag tcgttgcaga
    12781 taaaccagaa ccagcagcgc cagcagctcc tgcaatggat ccatcaatga tgggcggtat
    12841 gatgtaagaa aaagcaactt gcttatctac aactgatcat tagtgaaaga cagataaaaa
    12901 gaggctggaa cagaagcgtt taactccaag aaataagaag aaattcacgg aaattgcttt
    12961 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgctgtttat
    13021 acgtgtttag agcgtgagac aaaagtgttc tttacttttg tcccacgctc ttttttgtgt
    13081 gtaattataa tgaaaatagt tatttttaat agttataata ttttatctat taaaagtaaa
    13141 tatccattc
//
LOCUS       DAZUMK010000071        13032 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000071 DAZUMK010000000
VERSION     DAZUMK010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.073"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(124..486)
                     /locus_tag="ACG808_001882"
     CDS             complement(124..486)
                     /locus_tag="ACG808_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047504.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(560..940)
                     /locus_tag="ACG808_001883"
     CDS             complement(560..940)
                     /locus_tag="ACG808_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFS5047505.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1022..2197
                     /locus_tag="ACG808_001884"
     CDS             1022..2197
                     /locus_tag="ACG808_001884"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFS5047506.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2274..2858)
                     /locus_tag="ACG808_001885"
     CDS             complement(2274..2858)
                     /locus_tag="ACG808_001885"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS5047507.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3059..4057
                     /locus_tag="ACG808_001886"
     CDS             3059..4057
                     /locus_tag="ACG808_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS5047508.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4169..4678
                     /locus_tag="ACG808_001887"
     CDS             4169..4678
                     /locus_tag="ACG808_001887"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFS5047509.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4708..5367)
                     /locus_tag="ACG808_001888"
     CDS             complement(4708..5367)
                     /locus_tag="ACG808_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFS5047510.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5369..6133)
                     /locus_tag="ACG808_001889"
     CDS             complement(5369..6133)
                     /locus_tag="ACG808_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFS5047511.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6141..6818)
                     /locus_tag="ACG808_001890"
     CDS             complement(6141..6818)
                     /locus_tag="ACG808_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFS5047512.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(6855..8240)
                     /locus_tag="ACG808_001891"
     CDS             complement(6855..8240)
                     /locus_tag="ACG808_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFS5047513.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8450..8534)
                     /gene="ffs"
                     /locus_tag="ACG808_001892"
     ncRNA           complement(8450..8534)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACG808_001892"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8557..9072)
                     /gene="tadA"
                     /locus_tag="ACG808_001893"
     CDS             complement(8557..9072)
                     /gene="tadA"
                     /locus_tag="ACG808_001893"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFS5047514.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9179..10036
                     /locus_tag="ACG808_001894"
     CDS             9179..10036
                     /locus_tag="ACG808_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5047515.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10117..10452)
                     /locus_tag="ACG808_001895"
     CDS             complement(10117..10452)
                     /locus_tag="ACG808_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047516.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10519..11889)
                     /locus_tag="ACG808_001896"
     CDS             complement(10519..11889)
                     /locus_tag="ACG808_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFS5047517.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(11967..12503)
                     /locus_tag="ACG808_001897"
     CDS             complement(11967..12503)
                     /locus_tag="ACG808_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5047518.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12812..>13032
                     /locus_tag="ACG808_001898"
     CDS             12812..>13032
                     /locus_tag="ACG808_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5047519.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETD"
BASE COUNT         3950 a         2627 c         2158 g         4297 t
ORIGIN      
        1 tttgaaaata aaatgcacta aaaaactatc tttagcagaa catttcattg tattttttgg
       61 accaaacaat aaaaaaagaa gtttgaaaaa aattcaaact tctttgttcg cttaatctgt
      121 attttagaag aaaggcactc cctctacttc gtaagttcca agaacagagc gcatttgacc
      181 tggttcttcc caaatatatc cttcgcctaa ttcttctctt tgattctttt gttcagtttc
      241 aaagacaaac aacggaacat tttcctcttt aatatgtgta tttgtcaatt ccatcaaact
      301 aatcttgcta acatcgatat gaactatttc ttttaagaaa ttaagtatat tcgctaaacc
      361 agtctttcct tcagaaagtt ccgctaacgc aaattccaac ttgtcatcaa ttgaatggac
      421 caaaaatttt ttagtaccat cctccaaatg caacataatt gtaccagcaa ctcttttgct
      481 tgccaaaatt tccacctcga tcaacttgtt ctatggctta cataaaaatt ctagcacatt
      541 ttcagaaaaa atacactttt tatttcttat tttctttccg ttctagttca gataatgctt
      601 cttctatcca aataggtaac tgctccgcaa tagtggtaaa tccgatctta cgatttttat
      661 tcgtgaaata cctttttctg cgatatggaa cagctggatt tgttttttct aacgttctta
      721 aagataaact gatttttttg gaatattcat caatgtcgat gatctgaact gttactggct
      781 gtccgacttt taacaatgaa tggatatttt tcgtataacc ggattgtact tctgaaacat
      841 gaataagtcc ttgcgtctcg ttatcaagag aaacgaatgc gccataaggc tgtatccctg
      901 tcacttttcc ttcaattatt tgtccaattt tataagtcat ctacttccct ctcttcgcat
      961 ttatcattat actgtaaata gctcaaaaaa agaagcgacg aaaatgactg gaggtcttaa
     1021 gatgatcgaa tttgataaac agatttcaag aaaagataca gaaagtgtaa aatgggacgc
     1081 aattgctgag acatatcaaa tggatgactt acttcctttg tgggtagcag atatggactt
     1141 cttgtctccc ggtggggtag ctaaagcgtt tagtgaatac atcaaacatg gaatctttgg
     1201 ttatgccact ctgtctgata aactatatca ggctatcatt cattgggaac gccaacacca
     1261 tgctgtggag ttgacgaaag aaaatatcgt ttttacaagt ggtgtgttaa ccagtttagc
     1321 tgcagcagta caaactttta caaaaccagg cgattctatt ttgattcatg acccagttta
     1381 tcctccattt tcctctatta tagaaacaaa tcaacgtcac atcgttcgaa gctctttgct
     1441 agaaaaaaat aatcatttca tcatggactt gtctgacatg gagaaaaaaa tcgaagaaaa
     1501 caatgtaaaa gcaatgattt tatgcaatcc tcacaatcct ggagggcgtg tttggagcaa
     1561 agaagaattg aaacagttaa gcgaattgtg tttaaaacat caagtgattc tgtttagtga
     1621 cgagatccat caagatttaa ctttattcga tcatacattt acttccatgc taacgattga
     1681 tcctaattta gatgatctac tagttgcttt tacttccgca accaagactt tcaatctagc
     1741 tgcaatcaaa aattcaatgg tatttatcaa aaatccagag atgaaagctg attttgaaaa
     1801 acatttagtg atgaaccaac agcacgaaat caacactttt ggactgattg gaacccaagc
     1861 ggcttatgag acgggtggag aatggttaag ccaactgatt ccttatctag aaaaaaatgt
     1921 tcaaacagtt aatcagtttt ttgaacagta tcttccgaaa gtgagaataa tgaaccctga
     1981 aggaacttac ttaatgtggt tagattttac cgcctatatt tcagacgacc aagaattgga
     2041 gaacactctt gttcggaaag gaaaagtcgt attaaatcct ggcatcacgt ttggtccttc
     2101 cggtcatggg catatgcgct tgaatattgc ttgtccagaa gaaactttac tggaaggact
     2161 taatcgaatc aagtatgctt tagagacaat cgattaaatc atttattcaa aataaaagga
     2221 cgagacctta ctaggtttcg tcctttttca taattactcc gtaccatacc aaactattct
     2281 gaaatttcaa ttgtttcaat cactacatca tgtacaggtc ggtcttgtgg accacgttgt
     2341 acatttgcga tttcatctac tgtgtccatc ccttctagta catgtccaaa gactgtatgt
     2401 cggaaatcta gccaaggagt tccgccacct ttatacgctt caatgatttc tgatgggaat
     2461 ccagcacctt ctagttgtcc aagcatatta gctggcacat ttgtgttatt tacaataaag
     2521 aattggcttc cgtttgtgtt tggacctgaa tttgccatag ataaagctcc gcgaaggttg
     2581 aacagttcac gactgaattc atcttcaaaa ctttcgccat aaatactttc gccacccatt
     2641 cctgttccag ttggatcgcc gccttggatc atgaaatcag gaataacgcg gtgaaaaatc
     2701 acgccatcat aataaccttt tttggaaagt tcaatgaaat tttgaaccgt cttcggtgct
     2761 agttcaggga ataattgaac agtaatgtca ccacggtttg ttttgataac agcttttggt
     2821 cctttttcac tttttaaatc tagttgtggg aatgccatat tgtttcctcc taatatttgc
     2881 atacttcata ataataataa cataatacct aacctctcgt aaaaatacta gatttgtttt
     2941 cttttatttt tcatcactag atacattcta taacttaccg tttcgatttc gaattcaaac
     3001 atttttgaga agcattctca attaaattct gctatagtgt gaaaagggag gaatttttat
     3061 ggaaatctat gacattacaa tcatcggtgc aggtcctgta gggatgtttg ccgcttttta
     3121 cgcaggaatg cgccaagcta aaacaaaaat cattgacagt ctacctcaac ttggtggtca
     3181 attagcaact ttgtatcccg aaaaatatat ttacgatatt cctgggtatc cagcaataaa
     3241 agcaagtgaa ttaatcgatc aattagaaaa acaattaact acttttaacc atacattcca
     3301 tttaaaagaa gaagttctct cgcttactcg tgaagacgaa gttatcgaga ttactacaaa
     3361 taaaggcatc cattattcca aagcagtcat tttagcttta ggaaatggtt cattccagcc
     3421 aagaaaattg aatttagata atgctgaatc atttgaaaac cacggattgg attatttcgt
     3481 gaatgatttg atgaagtatg ctggaaaaaa agtcgctatt gctggaggtg gtgattctgc
     3541 tatcgattgg acgttgatgt tagagcctat tgcaagcgaa gtttacttga ttcatcgtcg
     3601 cccggaattt cgtgcgcacg aacatagtgt atcacgcttg aaatcttcat ctgtcaactt
     3661 gcttactccc tatctcatcg atggattatc aggcaataat ggggaactga cagatatccg
     3721 gttaaaaaaa gtgaaaagtg acgagacgat tgatttgatg attgattctc tgatcgtcaa
     3781 ttatggattc tcttctaatt tagaacatct ttcttcttgg ggacttgata gtaccagaaa
     3841 tgccattact gtaaaatcag atatgtctac ttctattcca ggcgtttatg cagctggtga
     3901 catctgtacc tatgaaggaa aagtgaaact gattgctact gggctcggtg aagcacctac
     3961 tgcagtgaac aacgctttac actatattaa tccaaaggaa cgaactcagc ctggtcacag
     4021 cacaagctta tacgataaaa atcttcgtcc tagttgattt tttatatctc tttgcatttc
     4081 aagttaaacc cgaaagcagt ttcttgcatt tttttcatgt tccgttaaca tgaaatagag
     4141 aaagaaaaaa taaaggggga gcaataatat ggtcgtcaaa actgagactt tagaagaaaa
     4201 ggcaagagaa ctattgaagg aacgtggcgt aactgtagaa gatatcgcac aacttgtcat
     4261 gtttttacaa aaagactata tttctgattt gactttggcg gattgtactg aaagtgtcaa
     4321 cagcgtgcta acaaagcgtg aagtacataa cgccattatc actggaatcc aattagatat
     4381 attagcagaa aagaaaaaat tgatgagtcc gttgcaacat attatcgaag atgacgaagg
     4441 cctttacggt atcgatgaga tcctagcctt gtcaatcgtg aatgtttacg gtacgatcgg
     4501 ctttacgaat tatggttata tcgacaaggt gaaacctggt attttaaaag aactaaacag
     4561 ccacgatggc gttcatgtcc ataccttctt agatgatatc gtcggagcga tcgccgctgc
     4621 tgctgccagt cgattagcac acaaagaacc agaaaaaaga agtcgcctta ctcaataatt
     4681 caacaaaaaa agaaagaaaa cgtctattta gacgagttct ttcttttttg tttgtttcaa
     4741 gctatttttt cctaagaaaa gaaaaacagc aaaaaataat tctaataata caaaaaataa
     4801 gataaagcta tgcatgaaaa attcttctgg aagtacattg atgattggat cagtaacggg
     4861 atcgaataac cagtcatcgt tgttgaaaaa cgtttcatga aaaaggataa aaaagcgatc
     4921 aaatcctatc atcatgaaaa aaccgaaaac aactggcagt aacatgccga tttgaaacgg
     4981 acgaatcaac cgccaaagcc gatcattctt ttgcaaatat ttgataaaca aaatactcgg
     5041 gatcaaagtg atgaaaaaca cagcataatc cactaaaaac aaaattttca cttcataaaa
     5101 gtgatgggct ccgctagcag aaactgggaa atctggcaag ctgaggatgg ttttgaaggg
     5161 attattgaga taattcatta ggtgatcaaa atttttcagt aacgtctctt gatctaaaga
     5221 agtataatcc aaaatttgca aatgtccgat atcaaatacg tatagtggtc gaaagttgat
     5281 cgtcacacca actgccagac tgatcagtgt cagaaatagg ctgaacaatc cagcatattc
     5341 catccactgc catctcgaat ttttcatatt agaagctcca ttcatccagt gattgtttca
     5401 cataggtagc tgggataggc aacccaggta catcttcttc ttttgtaaaa cctgataaaa
     5461 cgagcaaagt atctatatta ttgcgtatcc cagcttggat atctgtttca tagttatctc
     5521 caaccatgat tacttcttct ttttgcagcc ctaatacttc aacggcttta tccataatga
     5581 tggcttctgg ttttccgatg tagatcggcg gcgtttgggt tgctgtctct acaaaagaga
     5641 tgactgaccc tgctccagga agaaggccgc gttcagtagg tatattctta tctgggttcg
     5701 ttccaatgaa agtagcacct ttttgaatag caagtgttgc tttaactact ttttcatagg
     5761 tcagataatt atctaagcct accactacat agtcaggagc ctcttcttcc catgtaaatc
     5821 cggcagctaa aatcaagtca atcaatcctg cttctccgat aacatagact tttttccctt
     5881 ttccatctgc tttcataaaa tcgattgttg ccaaagtagc tgtgtagact gtctctggag
     5941 ctacatgaat gtcaaactca tctgctagac gattagctac tgtttctggt gacttagtcg
     6001 tgttattggt aacaaacaag aaaggtaatt ttcttttttg caattcttcc acaaatcgtt
     6061 taccggccgg tatgggttgt gtcccacgat aaatcgttcc gtctaaatca attaaatatc
     6121 ctttatagtc cattcatcca ctactcttcc ctttttctga tcgtaaatgc tgtttttgtt
     6181 ttttctactt gtttaggttg ttcttgtttt tttacttttt cagaggtttt tgctgactct
     6241 gtttttcgat ttttaataac cggttttttg cgattagatg cgactggtcc ttttttctca
     6301 tcgatatacg cacgattatg gttttttttc ttttttcggc gctgagttgt cttttctttt
     6361 ttaccgccga ttctctcgat gacaaaatac gcacaaccaa aattgcaata ttcgtaaaga
     6421 tagtcttcca aggaatcgat tcgctgatct ggatgcgccc gtcgattatc cgcttggaaa
     6481 aatcctttta ggcgtagttg ttcataaccc caatctccta cgatataatc atatcgggct
     6541 agtacatcac tgtaacgttc tccaagcttt tctgcattaa atccttcacg ataatcggac
     6601 acaagccgat aatgtctttc acctatcaaa aaattactgt catctaacag tgtcactagc
     6661 tcgcctttga ctttttctgc tttaggctcc tcaacgattt cttccaaagc agctgtcagt
     6721 tcttcggtga ctgttgtttc ctgttcagct gatgattttt tcttagcaat ttctgtttta
     6781 tgctcaacgt tctgctgttc attttgtttt gcagacatgc gttcaccacc tatctttttt
     6841 attatacctt gtttttattt gattgggtag tgagctttca agtaatctcc taccacactt
     6901 cggataaatt cagggaacaa aaattcattt tttccagcaa tctcaatcgt tggaaaaaat
     6961 ggaacaaaca tcaaatgatc tacggtagta aaagtataat ttttattcag atcgattggc
     7021 ttgttcttcc aaaagacttg atggttaata gaatcatatg tgattccatt atagaccatc
     7081 tgcccaaaaa tcttaccgcg aaatcccatt cccttcatag gatagttacg taaaaagtta
     7141 cgattttttt ctatttctaa aactaagcgg atcagatcat ttccacccaa tgtgacattt
     7201 aacaagtgca tcggatgcgg caacgcagtg tgaagctgat cctgattgac ccgccctttt
     7261 ggaatttcag ttaaaaacag accactgttt aatattgctg cttccgtctt tcctcgttct
     7321 ttcaccgcct caaaggctac ttggataaac gaatgctcat tgaagatatc tagactcaga
     7381 gaaaatggca aatctgctac ttctttttct tttaataatt catgcccacg ttctagataa
     7441 ctatttattt cttgttcatc ttctgtaaaa gctgtcattg tttcggtagg gatggctttg
     7501 gctgaatgct taaggatttt tttctcttca tcgatggtta aatggacttc accgacatac
     7561 tgcccgtact ttccagcggc tgctatttgg acatcattga ttacgtgacc ttctctgaat
     7621 aaatgatggg tgtgcgatcc taaaataaca tcaattgctg gtatttcttt tgcaatttgg
     7681 aaatcatcct gaatcccgag atgactcatc aaaactaaaa catccacttg tgaacgtaaa
     7741 tcttcgacta attctggcaa aatatcataa ggattccgta tgtcccagcc attcggatta
     7801 taagtaagcg gaaatggagc agtaaatgca attagtccga ttttcgtctg gtgtttcgtt
     7861 gtaatgattt tatatggttt cgtccatgcc ggtggctgga gcgtattttt atcgaataag
     7921 ttatccagga tgatatcaaa ctcagcatga tcgtataaat gattcaactc ttctttggaa
     7981 tttcctacgc cttcattgtt tcctatcgtc gctgcatcat aaccaacttc attcatcagc
     8041 aagatatttt cctgtccatt cgttgcttct gacaatggat gccaacgatc cacaaaatca
     8101 cctagatcta ctgcaatcgt tgtcgttttt tctttctttt cgttttctcg tttacgttga
     8161 tccaaaaacc ttctgatcct tggccaattt tctaaatgag aatgcagatc attcgtatgt
     8221 aaaatcacta ttttttccat gtaccctctc gcctctcttt atctttatca taccataggc
     8281 agctttttct tccattgaaa caagctctat ttcagagttt ttcctgcctt gaagcaaatt
     8341 gtataagtac aatattcgat aaatcagtct ggctaaaaaa tcaggaacat tcatttttac
     8401 agtactataa aaataaaaaa aacagacccg aaagtctgtt gtcgtttcat tataatcaga
     8461 cacatggcgg aacctgctta gtgctgcttc cttccagacc tgacacgctt cacaagccca
     8521 ccattgtcct agcctttcgg caaagaatag tataccttat ttttcattcg ttgaccagtc
     8581 tttttttaat ttcttttttc gctggcggag ctttcggaaa aagtcagtca aaatcatccc
     8641 acattcgtct tctaaaatgc caccttctac atatgcttgg tggttaaacc gctcgtcctc
     8701 caaaagattc atcaaggtac cggctgttcc gccttttgga tcatacgcgc cgaaataaac
     8761 ttctgctaca cgagacaaaa tcatcgcgcc gctgcacata ggacaaggct ctaaggtaac
     8821 aaaaagtgca gcatcttcca agcgccagct tcccactttt tcacaggctt ccttgattgc
     8881 aagcatttct gcatgtgtag tagcatcttg cgtggtttct ctaaggttat atcctcttcc
     8941 aatcactttt ccatccaata caaccactgc tccaatcgga acttcatgaa gcatttctgc
     9001 tttctttgct tctgccaatg caaggcgcat ccatttttct ttttctactg catcaagatt
     9061 tgtatttacc aattaccctt ccctgccttt gttttttctt gttttacttt agcatatgat
     9121 aaaaaggctg taactaactt ttttttctat ggtaaaataa atacaagagg tgaacaagat
     9181 gaaaatggat aaattacttg ctctttaccc acaagcacag aaaaaaaact ctccagcatc
     9241 agaagcggat atcctttctt ttgcaattga tgatgctttt atctggatca aacaagattc
     9301 attatctcaa caagaaacca gccttttgaa agcgttattc cctgtaatca acgatcaaaa
     9361 aaaacaccca tggtatcact atctttttaa agatgcttct tgtgttcaag aaaaaagctt
     9421 tcgtttgatc cagcttcacg tcgaatcaaa aggagaattc ttaaaaaaag aatggcaaga
     9481 tactattttg gaattatttc gctattctgc tgatttcttt ttttatacag acacagatgc
     9541 gctcttagtc gaacaaataa accgtgatta tcttgatgct actgaaatca acggtatttt
     9601 tctatcttta gatgctgatt ttgatacgac aacgtctgta ttcgttggtt ctttccattc
     9661 acctggcacc gacctcgtcc ctttgtttgc tgaagaaaga aaaatttttt taacagagca
     9721 aaacaatctt tatactcaca gccgtacttt ttctatgcaa gatgtggctt tacattacta
     9781 tacaaaagat actgtaaaag atagtaagtt gatcaaaagt tacatagaac tgatcaaaca
     9841 aaatgaaatg gaagtgatca ttcaagaatt atggaatgaa ttaggcaatg tcagctctgc
     9901 tgcaaaaaca ctgtttcttc atcgtaatac tctaaaatat cgaatcgaaa aatttcaaga
     9961 acagtctggt ttcaatctaa aaaaagccaa cgatttactg ttttgctatc ttttattgct
    10021 tcatgaacat aattaaagat agattaaagg ggttgtgaca ataattttgt cacaacccct
    10081 tatttcgaat gaatagtgtt caaaagctag tttctcttac atcatttttt caacaaaatg
    10141 cattagccgg tcttttacat cttcggttga atctttgatt gaatcaccat aaacagagat
    10201 tttctttttt cctgatttga tttttgctaa cattcccatc aaagaatcca agtcactatc
    10261 tgaaagacca tctacgattt ctagtaattt ttcatttcca tcaaatttgt catagacgaa
    10321 tttttttact ttcgtccgag tagtcatatg atggatcttt tccattactt ttcccgatac
    10381 aacgactgct gtcgctacac tggcagcggc tgctacgctt aatccgattg ttacttttgt
    10441 tgatgttttc ataagtatca gctccttttt atctatctcc atcatagcat agacaaaaaa
    10501 ataaacgaac taatccactc agtgaaatct caatccttta gggaaaaagt tctttactgt
    10561 tccttttact tgtttcccaa atccgacagg tatcttatct aatactagca aaacccatcc
    10621 ttgattccca tcacgttgaa atgtttctcc tgctgtgtaa gattgccatt ccttttgagt
    10681 gatcggcaag caaggtatat tctctatctt ttttgtagca agcgctaatg catagctagg
    10741 ttcaaatcga ttctttttga aatctcctaa atgcaaacct gtacgtacga cttttaaacc
    10801 atcgagagaa ggggctaact caggaacttc ccaaagatgg tcgttgaaca ctaaaagtct
    10861 tccagtagct tcataatgaa aatcatttga aaactccgtc cagagttttt cctgctcttt
    10921 tgtcatttgc actttacttt tttttcgtgt cttcttttct ttatgcatct gattttgtcc
    10981 atgaaaagtc agctttgcaa caaaatgtcc ttcaccctga tctttatgag gccatatacg
    11041 tattgttttt tccaaaccag ctacgcttcc ccattcagaa cgtcctgaac taacggattg
    11101 agtcaaagga atctcctcaa tcgttactgg ataattttct acaagccagc taatgatctc
    11161 ttcattttct tctggtgcaa acgtacaagt cgaataaatc agttgtcctt tattttttag
    11221 catttttatt gctgaagaca aaatctcttg ctgacgtttt tgacagtata gcggagattc
    11281 ttctgtccac tcttttattg catttggatc ttttcggaac attccttctc ctgaacaagg
    11341 agcatcaacc acgatccgat caaaaaagcc cgaaaaatga gggacaagtt ctgcgggtgc
    11401 atgatttgtc actattgcgt tcgaaactcc ccatcgttct atattttctg ataaaatctt
    11461 cgcacgtttc ggaaagatct cattcgtcac taataaccct ttccccttca tttgagcagc
    11521 taattgggtt gatttaccac cgggagccgc acacaaatcc aaaacttttt caccgggttt
    11581 tgctgcagca gctgttccaa cgatcattgc acttggttct tggctatatt catacccagc
    11641 ttgatggagg aaagactttc cattgacagt gccaagaaat ccctcattgc tgtagggagc
    11701 gggttgtaat tcttcgctat ggtaagtttg gaccatgtcc aatcctgccg gttttagtgg
    11761 attccaccga aatccttttt tcacgcttcc ttgttcaagc gcagaaaaaa aatcggatgc
    11821 ttcttcgcct aaaagaagac gatatttttt gataaattgc tgtggtaacg ttgtcgcttc
    11881 ctccttcatt ccaatgtttc gttagacatt ttacaatctt ttttacgaaa aataaaggtg
    11941 gctgatctac tttttcatgt tgctgcctaa ggtgactgtt cctttaagat attttccaag
    12001 tattcatccg tcacctcgtt tttatcccaa acagcgttca tcatttcaag aaaatcttga
    12061 gaagaatcat ctgagcgcaa acttcccaat gcattaagcc ataccaattc aagcgacttt
    12121 tcttctttga tcttcgtgtg acctttgaca tatgagaaaa atggccgctt aaatagcgaa
    12181 tccatgtcta aatcagattt tgcaatgatt tcacgaatac tttcatggat agaaaaaaaa
    12241 tctttcaata agtagacaat gcttaaaaca tcatcggaac tgcattcagc taagtagttt
    12301 tcaaaataaa cagtgatttc ttctaatatc ttctctttta acccgctgat tgatttgaaa
    12361 tgtgtataaa tcgggtatgt agaacaaccg cagaactctg ctaactctct tgcagtaatt
    12421 gacttcattc catattcttc cacgtatacc atacttttga acagcaaata ttctttacta
    12481 taatttgtta ctctccccat cattcttacc tccgtctctt ttcatctacc cataaataaa
    12541 taacataaat aattctaaaa atattattga taattatcaa aacatattta gttatatcca
    12601 ttaaaaatca atttttccgc cttaaaaaca agaatatctt gacaaaatac cctaggaaat
    12661 aaacaataga ataaacaagg taacaaaaaa attaaataac gttatataaa aaagaaggcg
    12721 acactctgtg agaataacac agactgaaat gactttccac tcacagtttt tgtctaacta
    12781 tatacaaaaa cagaataaaa aggaggtatt tatgctaatt gaagcaagaa aaaaagagtt
    12841 acttgttcta caaatggtca ttcaaaaaaa taattgttct ttacaagaat tatccaatga
    12901 cttacatatt ccaaaacgga caatcaaaga actgatccga aaaataaatg tatcactcaa
    12961 gcaattttta ttgattgatg aatttattta ttctaatcat aaaggagaga tccaaaccaa
    13021 agaaaccgat ca
//
LOCUS       DAZUMK010000072        13004 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000072 DAZUMK010000000
VERSION     DAZUMK010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.074"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(549..2180)
                     /locus_tag="ACG808_001899"
     CDS             complement(549..2180)
                     /locus_tag="ACG808_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFS5047520.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(2194..3867)
                     /locus_tag="ACG808_001900"
     CDS             complement(2194..3867)
                     /locus_tag="ACG808_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFS5047521.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(3880..4197)
                     /locus_tag="ACG808_001901"
     CDS             complement(3880..4197)
                     /locus_tag="ACG808_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS5047522.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(4222..5598)
                     /gene="lacG"
                     /locus_tag="ACG808_001902"
     CDS             complement(4222..5598)
                     /gene="lacG"
                     /locus_tag="ACG808_001902"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFS5047523.1"
                     /translation="MFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFSPDPASDFY
                     HQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEECKKRNVTPF
                     VTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEIYPVATNQY
                     LLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLETKYAATDA
                     PEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPEEDFVELKK
                     ASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIYKEGIPRTD
                     WDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPRIDYLRVYL
                     ESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPKESAYWYKL
                     VSETKTII"
     gene            5858..6643
                     /locus_tag="ACG808_001903"
     CDS             5858..6643
                     /locus_tag="ACG808_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS5047524.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            6646..7338
                     /locus_tag="ACG808_001904"
     CDS             6646..7338
                     /locus_tag="ACG808_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5047525.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7612..7983)
                     /locus_tag="ACG808_001905"
     CDS             complement(7612..7983)
                     /locus_tag="ACG808_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFS5047526.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(8383..8577)
                     /locus_tag="ACG808_001906"
     CDS             complement(8383..8577)
                     /locus_tag="ACG808_001906"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047527.1"
                     /translation="MNVAKVREDENEWKEFKSRYSINSTPTFTVYREGSIEKTVFWTK
                     ESGMSLAEVEEFLDYVSMQQ"
     gene            complement(8558..8716)
                     /locus_tag="ACG808_001907"
     CDS             complement(8558..8716)
                     /locus_tag="ACG808_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047528.1"
                     /translation="MDVDTSEILYDIEIDENLLDVAQEIDHQKQEAIRYAGRKGKRKY
                     LLRECSKS"
     gene            complement(8707..9045)
                     /locus_tag="ACG808_001908"
     CDS             complement(8707..9045)
                     /locus_tag="ACG808_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047529.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            complement(9973..10089)
                     /locus_tag="ACG808_001909"
     CDS             complement(9973..10089)
                     /locus_tag="ACG808_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFS5047530.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            complement(10565..11443)
                     /locus_tag="ACG808_001910"
     CDS             complement(10565..11443)
                     /locus_tag="ACG808_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFS5047531.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            complement(11790..12815)
                     /locus_tag="ACG808_001911"
     CDS             complement(11790..12815)
                     /locus_tag="ACG808_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS5047532.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
BASE COUNT         4146 a         2693 c         2132 g         4033 t
ORIGIN      
        1 ccttgatagt atcgcattct gttttataca ctttacgttg aaaacagagt gggaataaag
       61 agccaacata atatggaggg ggaatcccta taaatcaaca ttcttcaata gctaaaatgc
      121 ttgattttca ttaaaaataa gatggttccc taccaaaaga gactataata taagttaatt
      181 tgtttcattc ttatcataaa aagaagttct cgaaaaacga actgttttcc gagaactttt
      241 ttctatccat ctaagtgctc aaatcatatt cacgacggaa ttctaaggtt accctttctt
      301 aaacaaactt tatgttatct gcacaacttt ttgcaatttt ttcattgctt cttacgaaat
      361 cgttgaactc tctttagcat aatacaaagt gtagatgaag tctggtgctt tactcgtttt
      421 ggtaaaaatg actggaaatc gtttctggtt atttttggca aaggcgaggt gcaggttcga
      481 ttcctgcctt ctacgctaac gatgtggctg aagggaacct ccgatcttga atcgaaacga
      541 tccgcagtct attgtttcac caatgaaaca aaatcaacgt tcgtccaagc atttcctgaa
      601 gccttgctat aaaccccaat ttcacaaaca cctgttgtaa ttggaatgtc tttgattgta
      661 atttttgtcc attcacttgt tttaacaacc gcagtattta gttccgctcc accataattt
      721 ttgatatata actgattagt atcaaactct cccccagaca ttacataggc actcaatgtg
      781 taagttcctt tgcttatgcc actaacattt tgataagctg atgcctcgta tgcttgatcc
      841 tgccaaaaag ttagtttgta gtccccaaaa aaagtgcccg cttccgtttt gatcgtaccc
      901 ttagcggttc cttgttgaaa ccaggtttgc caattggaag gactggtcgt tggtccatcg
      961 ctttcgaaac ttgcattttt aactagattt atttgcgccc ctgcttgttt aaacgcacgc
     1021 aagcttggca aggcgtttcc agcaaaatca aacatcgctt ggttgtccca ttcgtttccc
     1081 atagtggatt gaacactgag ataattcata cctgcttgtg ttgcccaact cacatttccg
     1141 ttgtaccaaa gaggttccca atagaagaag cccgccccgc gattattcgg tactgcctga
     1201 atccgatcgg caagatccag caaatactct gcttgtcctg tcggtgttgg ttcatatccc
     1261 cccgcagctg cttcggcttc accaaaagca tttggcatag tatcggcatt cgctgttgta
     1321 tgcccataag ctgtttcaac tacgatcact tctttaccat accgttggct gatcgcattc
     1381 atattggttt gaagttcatt cattgaaccg tgccaatacg gataatacga cactccaatt
     1441 aaatcaaatg acacacctcg actcgtgatt tcatcaaacc accaagtaaa tgctcctgta
     1501 tcgccaccat cagctaagtg gagcattact ggagttgttt taggttgagt gtctcgaaca
     1561 gcttggatac cagacttcaa aaaagcagcc aatcgatcaa attctccccc gccttctccc
     1621 caagacttgc cataaggcca gagcattccg ctattcagtt cattgccgat ctgaaccata
     1681 tcaggataaa cgtccagctg cttcatttcc gacagaacgt tctttgtata gtcgtacaat
     1741 gccgtattca tttcttcaaa ggtcagacct tgccatgctt taggaagatt ctgtttccca
     1801 ggatcaaccc aaaaatcact gtagtgaaaa tccagcagca ccttaagtcc cagatttttt
     1861 gctcgttttg caaggtcaat cgtcgtttcc agatcatttg tccctgctcc ataactttct
     1921 ccattactat tataaggatc ctgccacaaa cgcagtcgaa catagttgac accattattt
     1981 tttaaaatct ctaaggcatc ttttgaaata tcgtcttcat aatattttgc accagatttt
     2041 tccatatctg acaggatcga aatatctgct ccgcgaataa attcgctggc agatgcttca
     2101 acagtttttg aaacagcccc caaagcccct aacacagctg caaataaaac agctttacgc
     2161 aaccaaattc ccattttcat tatggctcct cctttattga aattggttca gaataaactg
     2221 aacagctttt tcaggatctc tcgttaatcc gatatattct gctccttttg tcgcaaccag
     2281 cttgattcct aagcgatcgg tgtcttcttt gatatcatca tagtaagaat tgacttgcgg
     2341 tgctaagacc accatatcaa aatcctgcag catttcgtaa tgagaaccat aagcacccgc
     2401 cattgcactg atcggtgctt tcgattcaga tgcacctttt tttaaagcat tagctaacat
     2461 cgcacttgtc cctgcgccag cacaaagtac caacacattg atttctttat ttgcatcgct
     2521 gataacttga ctcgctgcca caggtacttc ctcacgtaca tcggcagaag caccttcctc
     2581 ggttgcgatc acctctgctt tttgtgcttc tttctctact aagacagcat cgtatgcttt
     2641 gcaaaatggt agatagatca ggaagtcaac gacgattaga accgctgcta gaacaaaagc
     2701 taagataccc atgcccgttc ctagaaccaa accgatcggt gctggagttg cccaaggcaa
     2761 aacgtacata aagctgttca tgcccaatac atcgacaaaa aacttgaaca ggcaaacgtt
     2821 tacaacaggt gttaacaaaa atggaataaa aaaataaggg ttcaaaatca tgggtgccgc
     2881 aaatagcaat ggttcgttga cagcgaaact tactggaata aaggaagctt tcccaactgc
     2941 cttcaactgc ttagacttag caaagagtag gaagataaaa ggaacaacca atgttgcccc
     3001 agttcctccc atagttccta caaaattccc taaacccacc gtcagtactt tagaagcttg
     3061 ttcaccggaa ttgaacaatt gcagattggt ttccacattg gcatagataa ttgccgcaat
     3121 tgccggctcc acgatcgacg gtccatgaac accgacaaac caaaacagcg ccatcgctcc
     3181 ccagataata gcgattccta agtacccgtc agctgctgta aatagcggct gcagcaccgt
     3241 aatgattgct tctgcaaatg agatttggaa gatttgtcga atgcctaaat caattaaaac
     3301 ggccgcaaat actgaaaaag agaaaggaaa aatatctcgg aaggtttgcg aaatcgttcc
     3361 aggaacttct ttaggcattt taatggtaat atctttcagc acacaaaact tatataaatt
     3421 aactgttaaa aatgcagcga caaaggaagc cagtagtccc tttgtcccca tatattccgt
     3481 tgaaaatcca ccttcaatcg gagagacgct taacaacagg aaacttacca cagaggccag
     3541 catcacagag gtggtgttga ttactttgtt tttcggtaag cgacgattca tcgaatcagc
     3601 caagcagcga gcagtggttg ctgaaacgag taaccctaca acacccatcg aataattata
     3661 gactttccat agccatgtgg agacagattc aggccattgg taaccaaaaa tctcaggaac
     3721 cgcagcaatc aaaatgaaaa tgcttgaaaa taaaatagct ggcatcgctg ttaaaaatcc
     3781 gtcacgaacg gctcctaaat aactattacg tgagattttt tcaaaaaatg gtttcccttt
     3841 ttcgatttgt ttaatgattc catccatagc gttcttctcc tatccttgat tctgtctgta
     3901 cagtacaata aagtcttgaa tcagatctct cagcaataaa gtggtcatta aatgatcctg
     3961 cccatggata aaaatgaatc ccatctccat ctctttccca gatgcttctt ccgctaaaat
     4021 ttttgtttgc gaattatgtg caagggttaa attttcgtcg gcttctttca acgccgaatc
     4081 aacatgttca aagtttcctt gtcttacttc tttcaataat ttcaataatg tactgcgagc
     4141 gtcgcctgaa tatgcaacga tctcaaaacc aagcatttgt aattcctctt tattcatctt
     4201 ttcttcctcc attatcactt attaaataat cgttttggtt tcactgacca acttgtacca
     4261 gtaagctgat tcttttgggt aacgcttctg ggtttcaaaa tcaacataaa acaagccata
     4321 ccttttgtta tacccatttg tccatgagaa aagatccata agcgaccaca aaaaataccc
     4381 cttcacattt accccggctg ttatcgcctt gcttaaggat tccaaataga ctctcaaata
     4441 gtcgattctc ggctgatcca taataatgcc atcttcaaac tgatctttat accccatccc
     4501 gttctcagtg atatagattt tgttgtaatg tgggtaatcg cttttaattc gcagcaacaa
     4561 gtcatataga ccttctggat aaatcagcca gtcccaatca gttcgaggaa ttccttcttt
     4621 ataaatacgt tctccgattc ccttcacttt ataaacagag gtcccttttt cacccgtacc
     4681 gttatgatgg attgcatttt ctccatcgta agctttgaca aaatggcatt ggtagtgatt
     4741 gatccctaag tagtcattac gcgtggatgc ttttttcaat tcaacgaaat cttcttcagg
     4801 aaaatgatag ctggcctgat ttgcttcaca tatctcatca agagcagtca aggtttcagt
     4861 tgaataatag cctaaatagg tagcatctaa taagaaacgg atcgacaatg catcgtctaa
     4921 aaaggcagca tgcttatctt ccggcgcgtc tgttgccgca tatttcgtct ctaatgagtg
     4981 aactacacca atttcacccg gaagttcgtt ttctttgaaa tagttcacta cacgggcatg
     5041 agcaaccatc atattatgaa gacaggcgac gatctttgta aaatcatatt tgattcctgg
     5101 tgggaaaaca cctaacaaat attgattggt tgcaactgga tagatttcat taaaggtact
     5161 ccacaccttg acttctttga attcatgaaa acaaaaaatg gcataggaaa caaatgcttc
     5221 tattgtttct cgattcaaaa aatctccatg gtcaaacaaa cgtttagggg tatcaaaatg
     5281 atgcaacgtc acaaacggcg ttacgtttct tttcttacat tcttcgaaca cacgatgata
     5341 aaaagcgatt ccttctggat ttggtttccc agctccatca ggaaaaatgc ggctccaagc
     5401 aatggataat cttagaccat ttacaccaaa acgttcacac aattcaatgt cctttgcata
     5461 ttgatgataa aaatcacttg ccggatcggg actgaatcgc ccttgttcct ctaaaaagtc
     5521 atcccaagca acagcgcctt tgccaccttc cttagtggct ccttcaacct gataggctgc
     5581 tgtggctccg ccaaaaataa aatcttctgg taattaagca tgtacatctc tcctcaaatc
     5641 tataatcagt tttcgctctt tctaactgat tgcattgtaa acgcatccaa aacatttgtc
     5701 aacaaaaaca aacacaaata atcataaaaa ccagaaaaca gcaataaaat ctattgtaac
     5761 aaacataaaa accaccctaa atgtttactt ttgttcgaat tagattgact gttgtccgtt
     5821 tatatgatag attatcctta aattagaggt gaagacaatg ttgaaaaaag aaagattgct
     5881 tactatcgta gaaatggtca ataaaaaagg aatccttacc gttaatgaaa tcatcaatca
     5941 attagatgtt tctgatatga ccgttcgcag agatttagat gaattagaaa aatcgggcaa
     6001 gctgctgcgt gtccatggag gggctcaaag tctttcctac actttagatc aagaactttc
     6061 tcacactgaa aaagccactc tgcagattca agaaaaaaaa gaaattgctg ccacagcagc
     6121 ctccttgatt cacgagggag aaacgatttt cttaggacca ggtacaacaa tcgaactatt
     6181 ggcgacgcat ttactgaaca agaaaattcg gatcattaca aataattatc cagtttttga
     6241 tattcttgct caagcagatg gaccagaaat tctactgatt ggcggtgatt tccggaaaaa
     6301 taccggcgca tttgtcggac cgatcaccaa cgacaattta agaagactaa agtttacaaa
     6361 agcatttatc agttcaaatg gcgttcacaa cgaagcgatc tctacttaca gtaccgaaga
     6421 aggagaagca cagcagatcg ctctaaataa ttcccgtaag aaatatctac tcgttgataa
     6481 taaaaaattc aatcgagatg atttttatgt gttttataat ttgcatgatt tcgatctgct
     6541 cattacggat cgccagatct cgcgggaggt tcaagagcac tatgcacaat atgtcgatct
     6601 gcaaattgct gattcaaccg aaaataccac tataggagcg taactatgaa agggattata
     6661 tttgatttca acggaacaat gtttttggac tctcatctgc atgaagctgc ttggctgcat
     6721 atgatacatg atcatacaaa agatggttta tcagacgagg atattttaaa aaatattcat
     6781 ggtcggacga atacggagat tttgaaacat ttcatttcag agcatttgac cgcctctgag
     6841 atcgccgtat tgtcagaaga aaaagaaagc tattatcgta tgctttgctt gcaaaatgaa
     6901 aacgagcttc agttgacaaa agggcttgaa gcgactcttg accagctgac tgaccaacag
     6961 attccattaa cgattgccac ggctactgtc aaagagaatg ttgcttttta ttttgatatt
     7021 ttcgatttag cccgctggtt tgactgggac aagattgtct atgacgacgg ctctttccct
     7081 gggaaaccgc aaccggatat ttttcttaaa gcggcagaca aactgagctt agctccggct
     7141 gattgtcttg ttatcgaaga tgcttactca ggattacttg ctgccaaacg tgcggggatc
     7201 ggaacaatta ttgcaattga tccattcggt aaaaacaaaa cagtatttga acaggcacag
     7261 ctaaataaag atggaaccat caaagatttt actggttttt ggacagagca cctgtctgcc
     7321 acatccgtta attcttaaac gattcaacta actaccaaag aaaagcaact gaaatgcaca
     7381 cctcctttta taaaaaactg ccagtgaaac gacatgaaag atacactgct atatccttag
     7441 tgaaatatag cagtgtatct ttgacttttt cattctttta accatatgac gcgttcatct
     7501 ctatctataa ttaacattaa aaatagtgtt ttcacattat ttctcaaacc aatcttaaaa
     7561 atcatcagga gtactttttt gatatacttt tattaatgct gggcgtaatg gttaaaagat
     7621 ttccactagt tcctctccac caagctgagt tgaaaaatgc ttatctgtaa acaacataga
     7681 caaagaggaa ataaacgttt ccgtatcttt ctctgtccca aggatattaa ttaattttcc
     7741 tactaggtaa gtatcattaa acttaagatc tgccttcaca tcaaaagttt tgataaaagc
     7801 ttttatcatg cgtacaattt tgtctttatc gccataattg atttcaccac tcatatcaat
     7861 atagtcacta atccctgaag aaataatggc aactgcttct tctttcgcct tatcagataa
     7921 ttcatttaat gactgggatt gattgatgac ttcttgagtt ttatctgtta cgtaatctct
     7981 cattatccta tcggcatact ccgcaaagta atcttgaaaa aattcttcta cagggtaacc
     8041 aacttcgttt tcccaaccag gagaaatttc attatcagta cttattaaat ctattaaaaa
     8101 ccaacccgtt ctctgagtgt tgattctata atatttttaa tttcctatat aaatcaaaaa
     8161 tattatagaa tcaataagct aattaagaaa caaactttag aaactctaga agaacttctg
     8221 aatttatata gaagtgtgta gctacttact aaatatacaa aagttaaaat aaaatactct
     8281 ggatactctt tatactgcat taaagttagt aatattaaca gcaaagatat cgaaaaaaca
     8341 ataacataat atgtgtcaaa atttttcata ataagaactc ccttactgtt gcatactaac
     8401 ataatccaaa aactcttcta cttcagctaa agacattcca ctttctttag tccaaaaaac
     8461 agttttttct atcgatcctt ctctgtaaac tgtaaatgtc ggagtcgagt taattgagta
     8521 gcgactcttg aattctttcc actcattttc atcttctcta acttttgcta cattcacgta
     8581 ataaatattt tcttttccct tttctaccag catatcgaat agcttcctgc ttttgatgat
     8641 cgatttcttg tgctacatct agtaaatttt catcaatttc tatatcataa agtatttcac
     8701 tagtgtctac atccacaata tatacttttt atcagtagca atttttgagt aatccgttaa
     8761 aatgatctca gtctcgtcac tttcatcagt ataaatatac gttaacaatg aactcacctt
     8821 ttcaatcggt gttagctcta agcctactgt attttttact attgagtcat tatttaccac
     8881 cataacttcg tactgtatat accatcctat ttggtttcca tcaatatcag taattggctc
     8941 actagctaat ggagtctccg gaacaatatt ttcctgatat ttcttaaact cttctgtagc
     9001 acgaaaaagg ttatcatgtt cctcacttga aactggttga gtcatagaaa atgttacttg
     9061 gctaaacaga gggaaaataa acactacaat caacaaagta taaaaaaaag cccttctttt
     9121 tcatttttta tcttccaatc acaaattgaa aaacttattc ctgcaacttt gagtctagta
     9181 tcacaatcga ctgactacaa ctattcattt acattctact ttaagttcct ccttaaccat
     9241 taattttatt aaagatactt gtaatagtga ttctcacaaa atttcatgaa aaatacttta
     9301 ttactgtatc taaaattcca atacacaact tatcgactat tatctgaaaa ccaaaactta
     9361 ttattccttg atgttattct agcaatagta actgcttttt tatttttttg catatttttt
     9421 cggcgttact ttttaaagtt aactttttcg acaaatagaa agagatacga atcactgatt
     9481 tttttgaaga aaagaaatgg agtgagctga acgttagaga tattgggagc tttcgtaact
     9541 gcttattaaa cgataatggt tatttgcaac cgtattgctc gcttctacat gaaatatttc
     9601 atgaaatatt tcgcaaaacc tttgattatg tagttacgtt tccattcttg actaaaaatg
     9661 tttcaaaaac gaacaaaggt gttaaaaggt aaagtgatca atactactgg tcaaaataag
     9721 aggttgaaag ggaattgaaa aataactaca gataatactt ttataaacgt acgtgctttg
     9781 tggttctgta gttaactata taactggaat tagggaatgg tatcaatttg aaaagaaaga
     9841 ttctaccaat ttatcatatg ttaaaaatgg aaaatcaaca gcagttcttt cggaaacctt
     9901 acacaaaaac cgattattga aaatagccga aaaacgtttt tcggctattt ttttaattaa
     9961 gtttccttct tcttaccttt tcccactatt ccgtttcaca tctttgatga tcttcagcgc
    10021 acgttttcgt gttttgatat ttgggctttt caagcggcgg tatgcacttg agatatctgt
    10081 tttttccata tggacgaccc actttcttct ttgattgtag tcatgctaaa aaacacaaat
    10141 cgaaacaatt acgatttgtg ttttaaatgg tatcaaaagt taaaatgaaa gtcaacttgt
    10201 taaaacagtc gatcagacta gctgattgac tcctatctct ggaaataaat tttcattatt
    10261 acgttttagc tacctaaaaa aggccctctt gtccgttaac tttaatatct attgttatag
    10321 ctagtttacc tattgaacgt actgtgtgtt cgatagaaca ctaaaaaaga ggctgggaca
    10381 gaagcgttta actccaagaa ataagaagaa attcacgaaa attgcttttc aaatttttgt
    10441 gaatttcagc ttatttccga aggagttgct tctgcttccg ccgtttatac gtgtttagag
    10501 cgtgagacaa aagtgttctt tacttttgtc tcacgctctt ttaaactatt tcgaagtact
    10561 aaacttatga agtaagaata cctaagccag agccaatgtg ctcgcctgca agcaattgct
    10621 cttttgcttg tcgctgtaaa ctcgtacttt ctggataatc atgtatttgg gcatctactc
    10681 gtccaaagat ccaagggact ccactatcgt cctgttcatc aactgataat aatcgataat
    10741 cggctgcaaa aagacagatt tttgactctt ttttcaacca tttagtaaga ttgggtgata
    10801 gtaaccaagt atctgtgtac atcacttggt aatggtaatc aggaaaataa agttcaaaaa
    10861 aacgaatggc actttcgtaa ctatctctta tcttattctg agataaatct gcatcggaag
    10921 gaatatgaag agaaaggcac tgctttcctt ctaatcttag atccgtgtac cctttcggaa
    10981 aatatgtcat ttcgtactcc aattcaccaa tccgaaagat caagccacta gtgtaatgcc
    11041 aaatccaaaa accacggtca aagcgatagc ttcctgttac ttggaaatgt tcatgaataa
    11101 atctagtaaa agcattcatt gttgccaaat agatagttct tggaatcttc aatggttgat
    11161 aaattttttt ccaactttga gaagctgcat atagataaat agctaatgct gcaaaaggtt
    11221 cctcttcata gactttgtct attttctttg cagtcgcctt tgctttatta ggatccatta
    11281 gttcatgact atattgctca aacacctcat caaggatgtt ttttgcttgt acaatcattt
    11341 gctctataac gactttggga agagttaaag tttctgccag aatttggatt tcattgctta
    11401 ttttcttcat caaatttccc cttttcatat ctttgaacct cattgtttaa atgactgatt
    11461 atccggacac catatggtat aaacatactt ttattgtaca ctaactataa aacttaaact
    11521 ttctatgaag aaagaatatc tgtaagactt tcaaggaaag cattgaccat ctcaggaaag
    11581 tttattcagt taccatcata aacaaaattt tttgatagaa taaatataaa attttttgat
    11641 agaataaata taaaattttt ttgaaagttc ctttccacat ttttgtgtga atttgactta
    11701 atgctgagaa tctggctttt gaacaccttg aattagaggt tgtgacaaaa atcttgtcat
    11761 aacctctttt ggcacatata tttgtccttc tattctaatt tttgacgaac aaccgttaaa
    11821 taatgttgca aaccaaccgt cattacccac gcaacaccta aaccgatgaa acttcctatg
    11881 acgatcccta aaaaacgata ttcgatcaaa gaaatcaagt atgctccacg aataagtgta
    11941 gatagcatca acgccatcgg attaagaaaa atattagcaa ctgcataatt tctagcaact
    12001 agaaattgtg cgattccata tagaaaagtg atcagaaaaa tggtctgcaa ctgcgtaaac
    12061 ggcacaaatg acaagaatgc cgacacacat aacccaccca tcgatccaat cagatattgg
    12121 acttgtcgat gcttcatcgc atccaagttt tctgctagca aaatcgatgc acatgacaag
    12181 gtcaaccaat aaggattatg cagattcaag ccgtgactga cataaacgga aagaaataag
    12241 gcagcgctgt agaaaacaga atcaatgatc actaaaggct cttcgtgaaa tctttctttt
    12301 aaagttgctt tttcaattgt ctgctcaggc cgtgaatcca gtaattttgt aatcactgcc
    12361 gcaatcaggg caaaaacgac acccataaaa aaataaagtg agacgatcgg taattgagcg
    12421 attggcagct gcatgctcgt tcccatggca gaaagcatcg caaaaaacaa gccacctggt
    12481 ttagaaatat ggaataatcg caacacgaat ctgctggaaa aagcaaccaa agctacggca
    12541 aatggttcaa gccataaaac ataagtcgat aagatcccca aaacgaaagc aaccaacagt
    12601 ccgcaaccga tcagaaacat acgtttcagc aaatatttca tcggtatgtt ttgataatag
    12661 aaaaaagtaa agatccctaa tgatgaaaaa cttgaaatca aaaaatcatg atttatgtaa
    12721 cctaaccata atacaataaa gataagtagc atcagattca tgatacgaaa aggattatct
    12781 tgtattttat tgaaacgaaa ataagtcttg aacaatctat ccctccttcc tagtaattgg
    12841 aaaaaaagct gaaccaagat gaaatgccaa tcccgtttgt cagcattacc tagtataaca
    12901 actatttctt gatttttcat aaacttagta tcaaaaagta aacaacttgg agaaattaga
    12961 acaaaaaacg aacattccga gtcgttttga cagaaaaaag atag
//
LOCUS       DAZUMK010000073        12981 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000073 DAZUMK010000000
VERSION     DAZUMK010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.075"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(64..1173)
                     /gene="rpoD"
                     /locus_tag="ACG808_001912"
     CDS             complement(64..1173)
                     /gene="rpoD"
                     /locus_tag="ACG808_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFS5047533.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(1197..3038)
                     /gene="dnaG"
                     /locus_tag="ACG808_001913"
     CDS             complement(1197..3038)
                     /gene="dnaG"
                     /locus_tag="ACG808_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFS5047534.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(3220..5535)
                     /locus_tag="ACG808_001914"
     CDS             complement(3220..5535)
                     /locus_tag="ACG808_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS5047535.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(5720..6580)
                     /locus_tag="ACG808_001915"
     CDS             complement(5720..6580)
                     /locus_tag="ACG808_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFS5047536.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(6639..7271)
                     /locus_tag="ACG808_001916"
     CDS             complement(6639..7271)
                     /locus_tag="ACG808_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFS5047537.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTNLMQSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            7438..8199
                     /locus_tag="ACG808_001917"
     CDS             7438..8199
                     /locus_tag="ACG808_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5047538.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            8225..10816
                     /gene="mprF"
                     /locus_tag="ACG808_001918"
     CDS             8225..10816
                     /gene="mprF"
                     /locus_tag="ACG808_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFS5047539.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11004..12191)
                     /gene="tuf"
                     /locus_tag="ACG808_001919"
     CDS             complement(11004..12191)
                     /gene="tuf"
                     /locus_tag="ACG808_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFS5047540.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            12559..12915
                     /locus_tag="ACG808_001920"
     CDS             12559..12915
                     /locus_tag="ACG808_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS5047541.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         3727 a         2714 c         2309 g         4231 t
ORIGIN      
        1 tttttttctt ttaaaataaa ccaaacagtt ttactgattt ctcttctggt caatagtcca
       61 cgcttattct aagaaatctt ttaattgttt tgaacgagaa ggatggcgca attttcttaa
      121 tgctttagcc tcgatctgac gaatacgttc ccgagtaaca ccaaatactt tccctacttc
      181 ttccaatgtt ctcgtccggc catcatctaa accaaaacgt aagcgaagga cattctcttc
      241 ccgatcagtc aatgtatcta gtacatcttc caattgttct tttagtaact cataagctgc
      301 gtgttctgct ggacttgttg cttcctggtc ttctatgaaa tcacctaagt gagagtcatc
      361 ttcttcaccg attggcgttt caagggaaac aggttcttgc gcgattttca agatttctcg
      421 gactttttct gttggaagat ccatttccgc accgatttct tctggcgtag gttctcttcc
      481 aaggtcttgc agcaattgac gttggatacg tatcaattta ttaattgttt caaccatatg
      541 aactggaata cggatcgtac gtgcttggtc agcgattgct cgagtgatgg cttgacgaat
      601 ccaccatgta gcataggttg aaaacttaaa tcctttgcgg tagtcaaatt tttcaactgc
      661 cttcatcagt cccatgtttc cttcttggat caagtcaagg aactgcatgc ctcgtcctac
      721 ataacgttta gcgatactta ctactaaacg aaggtttgct tctgccagac gctgtttcgc
      781 ttcttggtcg ccttcttcga ttttcaatgc taacgctact tcttcttccg cagttaaaag
      841 ggagacacgg ccaatttctt tcagatacat acgaaccggg tcattgattt ttacaccggt
      901 tggcgcagaa agatcttctg tttttgcttg ttcagctttt ttctcagcac tcttcaagct
      961 gtgaattgat ggatcaccat tttcatccac cacactgata cctgcgtctt caatcttttg
     1021 gatcaatttg tccatttcat ctgcattcaa agtgaatggt gtagcaagct gattggataa
     1081 atcatcatat acaacttgtc gtttgggttt gttttctttg atgaaagctg ctactgctgc
     1141 ttcatacgtt tttttatctg ttccatttgc cataaaagaa ggcccccctt gagtagctat
     1201 ttggcttgtt ttaactgttt cgttaaattg atgatttcaa tcgtcaattc aagctcaagc
     1261 tgtttgtttc cagttcgtct ggcttcttgc tgcatttgtt tttttaatgt tatttcatca
     1321 gctaagccag atttgctgat cacatctagc aagtcttgga tttctcgtgg cgaactttct
     1381 tctgccaccg gtaaatattc gatttccacc accaaccgcc ttaaattttc atcttgcaaa
     1441 aagtctaaaa actctgcaag gttgaattca taatgttgtg agacatatgc atcgaataaa
     1501 aaataaactt ctgcatatgc atcatgaata aactgaaatt ctcgttcttt taaaatatta
     1561 cggacagaaa cttctttaaa tgcacgatat aatagaatct gctctgcctt ttctacttgc
     1621 gtcaaaggtc tctttttccg tgcagtttct tgtctcgtaa cctgagtgtg ttgactttcc
     1681 tgaggttctt gacgattttg ctgatttgtc cttcgaagat cgcgcagttg tttttgtatc
     1741 gtctctctgc cgatctgaaa ctctgttgac agttgagata gatacatatc ttgttcgatt
     1801 ggtgaaacaa ctcggctaag ttcaactagt aattcttcga ggtattcgat cttgtctttt
     1861 tcattatcta aatttttccc ttgttttaaa taacgtgatt taaaagtaaa gactgtttcg
     1921 cgcccatgag ccactaattc ttggaaagat gcttctccat acttacggag atattcatca
     1981 ggatcaagct tttctggaac gactacgaca gaaagtgcta atcgactgtt ttgccgaagc
     2041 aattcaatcc ctcgattggt tgcttcaaaa cctgcgttgt ctccgtcata gcaaaagacc
     2101 aattctttcg tcagcttttc catttgttgg atctgttgag ctgttagact tgtccccata
     2161 gaagctaatc cagttttgat accagcctgc catgcagcaa tcacatccat aaacccttcg
     2221 aacaacaaaa tttctccgtt tttccggata tcgcttcttg ctttatcaag attaaataaa
     2281 acttgtcttt tattgaacag ttctgtttct ggactattca agtatttggg ctgatctttt
     2341 tcctgccctt tttcaggtgc aagccagcgt cctgaaaaac cgatcgtctg gccttgagaa
     2401 ttacgaagtg gaaacatcac tctttgataa aaacgatcag acatacggcc atcatcatgc
     2461 gtgacaaaca atccagagcg ttctaacaat tcatttgaaa ttgattcatt cttgaatact
     2521 tgctgaagaa aagaacgctc attaggtgca aaacctatgt tgaattcttc aattaattct
     2581 aaagacaacc cacgactttg caaatattct aaagctggct gtccagcttt tgtatgcaat
     2641 agcatgtgat gatagatttc cgctgctttt tcatgagcag caatcagttt ccccgtagca
     2701 gattcaggat tttggacagc tatagtacgc tgacgccatt gatcttctaa cggtatctgt
     2761 tcgaagtctg ccacttttac cactgcttct ggaaaactta gaccttctag ttcttgaaga
     2821 aaagtaaaca cgtttccgcc tttaccacaa ccaaagcaat gaaaaatctg cttgtcttct
     2881 gcaacagaaa aagacggtgt acgttcttca tgaaaaggac acagccccaa gtaattttta
     2941 ccagactttt tcaactggac atattgtcca atcacctcaa cgatattcgt ttgactgcgt
     3001 actgtttcga taacctcttg aggaatccgt tgagccattt tttcaccccc tgctctattg
     3061 atatcttttg ggtttaacag aagatatttc aggagaataa ccggtaaaaa atcgcactaa
     3121 ctgaaatcag tgcgatttta aaggacacaa ttatacatgt ttgattgtat catatttcat
     3181 tgctttttca agtataacac atttctatct cattaagcat caatttgcta ctcttttcag
     3241 taactgattt tgaatatcag ccttccagta caatactcga agaacaatat ttacgagcat
     3301 gaatacaaag aagtatccta cgctctctcc caaccctaag tttacttcca aaattccatg
     3361 gaacagcatt gcagctacat atatccctac gatcgtcgtt actgctagga agatgcgcaa
     3421 tgggttgaag ggataacaag cttttacaac tgccatgcaa ctgatcccaa tcagcaagta
     3481 atacatcaaa gtagtagttt cagcatttcc ccatgcgaat gtttttccaa gtagatacac
     3541 cgcaatgata ttcactacta caagcaaggc atttggtaaa gcattaataa gtgctgtagg
     3601 caaataacgg taagtgattt ttcttttatc ttgttcaaaa gataaaaaga aggaaggata
     3661 cccttcaatc gctaaatcaa tcagcgtgat ttggataggg ataaacggaa atccaattgc
     3721 tgtaacggca catataatcg ataaaataaa tgaataaatc gttttgataa aaaagatgcc
     3781 tgagactttc gtcacattgt tgaccactcg gcgtccttca aacaggactt ctggcaatgt
     3841 agtaaaatca gagtccaaca ataccagatt cgcaatctga cgtgttgctc catcaccttc
     3901 tgccatagca atgctgcaat ctgcttctct taacgccaaa acatcattca caccgtcgcc
     3961 tgtcattgca accgttctgc cgctttcttt caattcattt accagcaatt tcttttgctg
     4021 cggactcact cgtccaaaaa ccgtatagcg atgagcagct tctcgcactt ccatttcttc
     4081 agtaatttga gaaagatcga catacgcatc ataattcggt aatcctgcac gtctagcgat
     4141 attggagaca gtaaaaggat tgtctccaga aatcactttc agatccacac cttcctcatg
     4201 aagataagct agtgtttctt gagcgttttt tcggattgga tcatcgattt caaacagagc
     4261 aaccggctca acaaatgctg gtttttcttc gttcaattgc tgatttggag cgatccccaa
     4321 cataagtacg cgatagccat tcgcttgggc tgtttttaga ctgtccggaa gccgatcttc
     4381 ttccaccaaa cgttcaggcg ctcctacgta cacgactccc agttctggaa attccatcgc
     4441 gccccatttg cgatcagaag aaaatgcgac tttgtttgtc acatcatacc gattagaaag
     4501 ctcaaaataa tctctcatag cccgcatagt gatgttgtta tcactgcttt ctgccagata
     4561 cgtccccata atttctggga tggctttctc gtacagcgga tgtagtgctt ctacccgctg
     4621 gatactcatt ttcccttcag taatggtccc tgttttatcc aagcataatg tatcgacatg
     4681 ggccagcgtt tcaattgaat acatgtcttg aacaaggatt cgtttttttg ccagtttggt
     4741 tacccctgtc gttaatgcaa tacttattag caataccaaa ccctttggaa gcatgcctaa
     4801 caaagctgct gcagatgcaa taacagaaat tttgatagaa gcatcgcgga tgaacagtgc
     4861 ttccaaaaat agtatgatcc ccaaaggaat aatcacgaaa ctagtgaatt tggatacttt
     4921 gcgaatcgaa tctacaagtt cagaacgaat agctttgtga gtctttgcct cgctagtcaa
     4981 gcggacagca taattatctg cacctactcg gataacttca gccatgacag aaccgcttgt
     5041 caaaaagctt ccagaaagca attcagctcc agtttctttg acaatcaaat cagattcgcc
     5101 tgtcaaaaga gcttcatttg cttctgcccg tccagatata acctgtaaat cggctggtat
     5161 ctgttctcct gctccgattt ttaccaaatc tcctaatacc agctctttag tagaaatcga
     5221 ttgttcttta ccgtcccgga tcactagtac ttctgattgg gaaacaatcg ataatttccg
     5281 tacaagattt cttgcatgta tttcttgata aattccaata acgatattga cgacaataat
     5341 tgctaaatac gccatgttgg aatacgcacc tacaaataac agacaaatcc caataccaaa
     5401 gttcagaaaa ttgaataacg tcaatatatt atcgctgaat atttgttttg tactttttga
     5461 tgcatcttcc tggtagtcat tttgctggcc agaagccatt ttctgaacta cctcttgctg
     5521 tgtaagtcca ttcattttct ttttctccat tttccgccaa ctttcaaaaa taatcacgtg
     5581 tttaagtcta gcgtgttcca agattaaaaa caacttgttt ttataaaatt tatagaaaaa
     5641 cacacgaatg attttacaaa aaagatgcca acctttttgt ttgaatcacc cgtgtgctgt
     5701 ttttattatc aatctatctt tacttatatt gttcctccaa acggctcatc caccaaccta
     5761 gtgctgtacc ggctataaaa agaacaggac aagctagtac cccagcaact cccaatcccg
     5821 cgtaatcggt aggaatgatc ataatcgttg aagcaaaaac tacacctaaa atgaaatgga
     5881 atagcttgct atatgccttt gagaaaatgt aatccatgat ttttgacaat ccaagaacac
     5941 aaagaatccc accaattccg atcggcacaa tcacttcaag tcgtacttct ttaaatccgt
     6001 cagccatcgc tttatacaat cccatataca ccaaaaaatt agaagggctc aatcctggga
     6061 cgatcatacc caaaccaatc aatccgccgg caatcagcca tgttccaaaa ttctgatcga
     6121 cttgcccgtc aaataattga gcgccgtata ataaaaacaa ataagccaga acaaaagtaa
     6181 caaccatgat gatcacatca gaagtatctc ttcctttttt ccctgcttct ttccataacg
     6241 caggaacagt tccagcgata catccgacaa aaaaccataa tatcgttgtt tcatacgcac
     6301 ctaataaaaa actgactaaa aaagaaagta gaaagatgcc tgtcactccg cctagaccaa
     6361 caggcagaaa aaacaaaaca ttttctttga aattttttgt aaggtgggct aaaaaagaaa
     6421 tcagccgctc atacatgcca aagacagctg ctaatgcacc accgctgact cctggcaaga
     6481 taaatcctga accgataaac attcccttca caaaccgtaa catccagtct ttggttgtgt
     6541 accccacttc ttgattttcc attttttgcc ttttttccac tatttgcgca ctccttcttt
     6601 catttaattt tattcatgat tgaacatttc ctgctatttt acttaaagac tttttcatag
     6661 gtctctatta ttttttcatt cattttgaca atatcaggat aaaacatgtt acctccatta
     6721 tgatagagat agccgccatt gtaaagcaag gactggatcc gccaatatcg gtattgtttc
     6781 ccttcctcat ttcctaaaag tgggctcagc acatctttag aatacgcttc tgctaattct
     6841 actgtgtttt ctccgccatg attttttaca taggcaatat aatctttccc aaaattatat
     6901 gcttggacag ctgtccataa atcgcatcct tgtttctttg cttctactat catttccgcc
     6961 aaatatttga caccttgatc aatactgtca gctggattat caatgatctc agcttctcca
     7021 tgcagacttt ccgagctctg catgagattt gtcccttttc cttttgattc ggtcaatatt
     7081 atcgccttga tcaaattctc gtagttttcc atatgataat tttttgcagc tgttgttatt
     7141 tctatttgat agctttccac tcgttttacc gaacgataag ttcttcctcc taaaaacaac
     7201 accgcaccaa taaccgtcag aatgatcatg taaaaaagaa tacggataat agataatcgt
     7261 tttcgcttca tttcatcacc tagttatttt gacatctttt tttatcttac tatttcttta
     7321 tcttcttggc tatttcttcc ttgatttctg tgtacgcctt aagaaaaaat gaattttcct
     7381 gtattttttg acatcttacg aaaatgatcg ttgtttttca gtaaaaacaa ttgcccaatg
     7441 actgatataa tcgttatact ttacacagta ggtgattatg tgaaaaaaga aaagaaatat
     7501 gcaaaaatgc cagatgggag cgaaatttac tatgaaaaga gcggtcaagg cttccctctc
     7561 tttttactgc atggcaatga cggcagcggc cgcttttttt cagaacaggt ccctgtactc
     7621 gaacgctact ataccgtgta tctagtagat agccgaggac atggtcgttc gacaaacgaa
     7681 gcatctatgt tgaattttca gttaatggcc gaggatctga atacgatcat gttgctggaa
     7741 aaaatcgatc aggcagattt tcttggattt agtgatggag caaatctagc actggtattt
     7801 gccagcagct tccctaaaaa agttcatcga ttgattttaa attctggaaa cacgcttgtt
     7861 aaaggcgttc gtttttctgc cagagtcatc agtaatattc attatgcatg ggtatggtta
     7921 ctctctttat ttcgcccaag tcttcgcaaa aatcttttag ttatcaaact tttgctgcat
     7981 gacatcgggc ttacagaaaa tgatttaaaa aaaatcaact cccccacttt gatcatcgtg
     8041 ggaaaaaaag atgtaatcaa gctaaaacat tctttgtata tagctaaaac tataccaaaa
     8101 gcttcctttg tgcttgtgaa agaacaagga catgaattag ctagaaaaga tcctgaacgc
     8161 ttcaatcgag aagttcttca atttctttct gaaacataac tattaggaat agaaggtgat
     8221 ttatttgtta aaaaatacca tacaatggct gaaaaaccac ctaggactgt tcaaaacaat
     8281 ctttttgatt tctgtcattg tcattattgt cggtgaattg atgtccatcg gtaaaactct
     8341 atctatccag caattaggag aaacgtttgc cactatacct gtatggaaaa gcggactaat
     8401 gctattgatt ggtcttatct ctgtgttacc tatggttggt tatgatatta ttttgaatcg
     8461 gatgcttgaa caaaagcaga atcctcgtta cctgtttgag acaagctggc tgatcaatac
     8521 gatcaacaat attgccggtt tcggcgggtt tgttagtgtg gggcttcgct ctgaactata
     8581 tggacaaaag aaagagagca gaaacgtgat ccaagcatta tcgaaagttt tcttgttttt
     8641 gatggctgga ctttctattt acagtctgct ttctttttta cttgtagtct tcacgccagt
     8701 tgctcctttt ttgaagcaat attggatatg gttgatcggc ggcggtctat attttcctgt
     8761 cgtcttttta tttactactt tcaaaaagaa aggattcctt ggtggtacct cacttaaaac
     8821 tcaaggacag ctattgctag tttcattgtt ggaatggacc ggtgtcctgt ttagtttcct
     8881 ttctgttggt tatttaatgg aaatccggat tgatctgtgg caaacaattc cattattcat
     8941 tgctgcttca gtgataggaa tcgtgtctat gattccagga gaaattggga gtttcgatgt
     9001 aatgatgatt atcgggttgt ccgctattgg tgtgccaaga gaaatagtcg ttgtgtggat
     9061 cttgctctat cgcctgttct actacatcat tccgtttttg attggaatcg tctttttctt
     9121 caaaaatatc ggttccactt ttgatcagcg ttattctggt atccctaaac aattagcaac
     9181 agaaattgcg cataaaatcg tggttgtctt attatacttt tctggaatca tgctagtact
     9241 ttctgccact atcccgcaag cttttacaga gtttcgctgg cttcatagtt tgaacccatt
     9301 gaaatttcat tttattatcc aatttcctag tatactattg ggatttcttt taatcattat
     9361 gggcagaggg atcgcagcac gtgtgaaacg agcatatttg cctacaatct tcttgattgt
     9421 tctagcttta ctttatgttt tattaagtga ttttagtttc acaccggtgg ttttcttgtc
     9481 tattctgctt ctgatcattc ttgcttctaa aaacgaatta tttcgggaac aattgatcta
     9541 ttcatgggag tggcgtacga tcgatggaat cattattggt gcactgtcct tgctttacat
     9601 cgttattggg gtatacaatc tgccagattt ccctcatcgg cgtcaccatt ttatttcctt
     9661 tttcttattt ccttctgaaa agatttggtt ttcgggattg ttggccatca tcgctgttag
     9721 tttcatgatc gttctatttg ttcatttcct tcaaggagaa aagaaacaga ttggcgaagc
     9781 ctttaatgaa gaaaaggccc tgaagatcct tacaacttat ggaggaaact ccgatagtca
     9841 attgatcttc ttaaaagata agcggatgtt tgcctatgaa aaagacggtg aaccaactgt
     9901 tcttctccaa ttcgcttgct tcaataataa atgtattgta atgggtgatc cttctggtaa
     9961 gaaagaagat tttccagaag caatcgaagc ttttatcgaa gaaacagatc gactatgcta
    10021 tttaccagtt ttctacgaaa caagcgaaga gattgttatg attttgcatg aattcggcta
    10081 cgatttcatt aagatggggg aagaagctta tgtagacctg aatagtttta caacttcagg
    10141 gaaaaaaatg aagggcactc gtgctgtctt gaatcggatc gaacgagaag gatttacatt
    10201 tgatgtctta caaccaccat tttctactga gcaaatgagt actttcaaaa atatctctga
    10261 taattggttg ggttcacgaa aggaaaaagg gttttcttta ggctttttct ctgaagatta
    10321 tctacaaaga gcgcctattg cagttgtcaa aaatacccaa gaagaaattg tcgcttttgc
    10381 aacgatcatg cctacttaca cgaacaataa agtaggaacg attgatctga tgcgatatga
    10441 ttctaaaaaa gcaccttctg gcagtatgga tttcttgttc ttgcatttgt ttgcctatat
    10501 gaaagaagaa catatccaat ggttcaattt agggatggcc cctctttcaa atgtcggaac
    10561 ttccagaaag agttttttac aggaaaggat tgcatacttg gtttacgagt ttggttctca
    10621 tttttattct ttccatggat taagagaata taaaagcaag tacgctacaa actgggtctc
    10681 acgttatact ctatattcta gagatagttg gattgcatat gttatgattg ctttgctgat
    10741 tattgataat gcgccagttg agcagacaca aggtagaata agtggtttga aaaaatggat
    10801 acgaagaaag tattaaaaac tgcataccga tagatgaaca ctctcatgat gaataaaaga
    10861 gactgaagta aaacgaaagt tcatgcttct acaatctctt cttatacaat tgaagaacaa
    10921 agagcggtcc aaacagaaat cctgtttgga ccgctctttt attttaaaca tttttcatta
    10981 ccaaaatatt cgagtcgcgt ttcttatgct tcgatctctg taacgatacc tgaacctact
    11041 gtccgtccgc cttcacgaat tgagaatgtc gttccgtttt caacagcgat tggatggatc
    11101 aagtcaacat caatcgtcac gttgtcacca ggcatgacca tttctgtatc ttctggcaat
    11161 gtgattgttc ctgtaacatc agttgtgcgg aagtagaact gtggacgata gttgttgaag
    11221 aatggagtat gacgtccacc ttcttctttt gtcaacacgt acacttctgc tttgaatttt
    11281 gtatgtggag taatggaacc tggtttagca agtacttgtc cacgttcgat atcgtcacgt
    11341 tggatccccc gtaacaacac gcctacgtta tccccagctt ccccgtaatc taacgtttta
    11401 cggaacattt ctacacctgt tacaactgct ttttgtgttt caggtttgat ccctacgatc
    11461 tctacctcat caccgacacg aacagcacca cgatcaatac gacctgaggc aacagttcct
    11521 cgacctgtaa ttgagaagac gtcttccact ggcaatagta atggtttatc tgtatcacgt
    11581 tctggtgttg ggatatattc atctactgta tccatcaatt ccataatagc agcttcagca
    11641 tctggatcgc cttgcaatgc ttttaatgcg gaacctttga tcacaggagt gtcatcgcct
    11701 gggaaaccat attcgctcaa taactcgcga acttccattt ctaccaaatc gatcaattct
    11761 tcatcatcga ctaaatcaac tttgttcaag aaaacaatca ggtattttac accaacttgg
    11821 cgtgacaata aaatgtgttc acgtgtttga ggcatcggac cgtcagttgc agaaacaact
    11881 aagattgcac catccatttg tgctgcccct gtaatcatgt ttttcacata gtccgcgtgt
    11941 cctggtgcat caatatgcgc gtagtggcgt ttttctgttt catattctac gtgtgcggta
    12001 ttgatcgtaa tcccacgttc acgttcttct ggagctgcat caatactagc atagtcttga
    12061 ggatttgcta ggcctttttt gcctaatact gtagtgatcg cagcagttaa agtagttttc
    12121 ccatggtcaa cgtgtccaat cgtaccaatg ttaacgtgtg gtttacttct gtcataatgt
    12181 tcttttgcca taatttacaa acctcctaaa taatatagat cactgaaaaa tttcagctct
    12241 caccttttta ttatagtctt tttttgacta aatcaaaaag aatacgtccg acttactttt
    12301 tgtaagattc gaactaagct tattatacgt caatattggt caaagtcaac taagagcact
    12361 cgtctgtctt atacgcgaaa aaagtacctt ttataaataa aaaaatgtgt tttttgagaa
    12421 aaacttcaag atagtcctta actttctttt gaagcaataa gaacaaaatg aggaaaagtt
    12481 taaaattcag ctgttgccaa caactttcaa tagacgaaat cttctatttc gttaataata
    12541 aggaaagagg tgaagtacat gaaaaaatta attggcgtac ttgtcgtttt aggcatattt
    12601 ctcggcggaa gttttgcagc ttatcattat ttttacggcg gcgaagctta ttatacaaaa
    12661 atcacgacga atggcgaaat gtcagctacc caagcagata acggagagaa attcacgact
    12721 tatacctaca agcaagcggc ttataataaa gatggtgaaa aaaaacaggt gacgcttcgc
    12781 gaagaaagag aacatccatt aaagatgaat gcgtatctta aactcaaagt caatcctcgt
    12841 aaaggtgtat taagctggga agaaataaag gcttctgaag tgcctaaaaa tgcggcagaa
    12901 aaaatcgata attagtaaat ggtcttaaaa ttttattaga taaaaagagg ctgggacaga
    12961 agcgtttaac tccaagaaat a
//
LOCUS       DAZUMK010000074        12965 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000074 DAZUMK010000000
VERSION     DAZUMK010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12965
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.076"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..450
                     /locus_tag="ACG808_001921"
     CDS             127..450
                     /locus_tag="ACG808_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFS5047542.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            632..2002
                     /locus_tag="ACG808_001922"
     CDS             632..2002
                     /locus_tag="ACG808_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFS5047543.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(2132..3433)
                     /locus_tag="ACG808_001923"
     CDS             complement(2132..3433)
                     /locus_tag="ACG808_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFS5047544.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(3663..5654)
                     /locus_tag="ACG808_001924"
     CDS             complement(3663..5654)
                     /locus_tag="ACG808_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFS5047545.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(5798..6064)
                     /locus_tag="ACG808_001925"
     CDS             complement(5798..6064)
                     /locus_tag="ACG808_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS5047546.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(6574..8700)
                     /gene="pnp"
                     /locus_tag="ACG808_001926"
     CDS             complement(6574..8700)
                     /gene="pnp"
                     /locus_tag="ACG808_001926"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFS5047547.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(8888..9157)
                     /gene="rpsO"
                     /locus_tag="ACG808_001927"
     CDS             complement(8888..9157)
                     /gene="rpsO"
                     /locus_tag="ACG808_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFS5047548.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            9408..9971
                     /gene="def"
                     /locus_tag="ACG808_001928"
     CDS             9408..9971
                     /gene="def"
                     /locus_tag="ACG808_001928"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFS5047549.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(10224..10745)
                     /locus_tag="ACG808_001929"
     CDS             complement(10224..10745)
                     /locus_tag="ACG808_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFS5047550.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(10748..11590)
                     /locus_tag="ACG808_001930"
     CDS             complement(10748..11590)
                     /locus_tag="ACG808_001930"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFS5047551.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(11583..12308)
                     /locus_tag="ACG808_001931"
     CDS             complement(11583..12308)
                     /locus_tag="ACG808_001931"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFS5047552.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            12484..12780
                     /locus_tag="ACG808_001932"
     CDS             12484..12780
                     /locus_tag="ACG808_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047553.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
BASE COUNT         3624 a         2713 c         2420 g         4208 t
ORIGIN      
        1 tcaattattg ttgcattttt cctgattctt tattagaatg aaccctcgtt gaagtaagta
       61 tttctagttt atcctaggat taattataaa aataaaacat tcgttgattc gaaggaggtt
      121 aaacagatgt ttgaaagttt tgatagcagc cgcaaccgtt acgcttcagt tggtgtcgta
      181 tcaagtttgc cagatgaatt gattgacagt atatggttta ttattgacct agatcttaag
      241 ggagtcattc cattagaaaa tatattaacc tttgatttga tcgaaaatcg tggaaaagtg
      301 acgatgcatt tttctcaagc aggaagcaat gtggaaatgg atattgattt gccgtttgcc
      361 tattcgaacc aatttcctgc tcaagtatac gcctatgatg acggtacgag agaaacgatc
      421 cttttaccaa gtgagattag acaatattaa ggtgatagat aaacgtggat gtcccttatt
      481 ttacagacca ggccaagttg ttgattcttt gactcaattc ttggcttctt tttatggagt
      541 ttcataaatt atcattgatt tctaattggt attctcagga taaacgctat atactactaa
      601 ctaaatttta aagatataag gagtttaaaa gatgcgcgat ttaacaaaag gaagccccgc
      661 taaactgatt ttgatgttca ctgttccttt actgatcgga aatgtttttc agcagttcta
      721 caatatgatc gatatgatca tcgtgggtca gacgctaggt aaaaatgcgc tggcagcggt
      781 tggtgcaact ggaagcctga catttttgat cattggcttt gcccaaggtt taacagctgg
      841 tttagccatt attaccgcac aacgttacgg tgcaaaagat tatcggggtc tgaagaaaag
      901 ttttgctgct agtgttgtca ttagtcttgt cgtcactgtg attttaactg tcctaagtct
      961 cctgtttatt cgtccgatgc ttcaattgat gcaaactcct cctgaaatac ttgatcaagc
     1021 acaaacattt atttcaatta ttttattagg gatctttgca tcagtcagtt tcaatttgct
     1081 ttctaatgtc attcgcgcac ttggtgacag ccgcacacca ctattctttc tgatcattgc
     1141 ggtcatcatc aacgtcgtac ttgatttgat ctttattatc tattttggca tgggtgtgga
     1201 aggtgcagct attgctacag taatcgcaca agtttcttcc agcgtactgt gtctagtgta
     1261 tatcaagaaa aaaatgcctt tgttacagtt acgcaaaaaa gatttcaaat ttgaaaaaga
     1321 agagtttgct gtacacctaa atgctgcttt gccaatggcg tttcaatctt cgatcatcgc
     1381 tgttggtgct atcgtgttac aagcagcact aaatagtcta gggacagatg tcgtagctgc
     1441 tcaagccgct gctggtcgta tcgaccaatt tgcaaatcag ccgatgatgt catttggtat
     1501 tgcaatggca actttcactg cacaaaacta tggtgcgaaa gaatatggcc gaatcttgca
     1561 aggggtacgt caaactttac tgatgagtgt cggatttagt ctcttagcag gtgctatcgt
     1621 cattttcttc ggtcatttct ttgttcaatt gtttgttagc ccatcagaaa cacgtgtatt
     1681 tgaattagca caaacctact tcaatatcaa tgggtctctt tactggatct tagcagtcct
     1741 gttcatttta cgctacacac ttcaagggct tggacaaagc aagatcccta cgatcgccgg
     1801 tatgatggaa ttgcttatgc gttcttttgc agctatcgtt cttacaggaa tgtggggata
     1861 cccaggagca gcagcagctt ctcctttagc ttggatcggt tctgttgccg tgttgcttta
     1921 ctcttatatg cgttcgatga aacaattgaa acacatgggt gaagaggaac atttcacatt
     1981 agaacatgca gaagcaagat agccttgatt aaaagaaatc gttgcggcta aaaatgaaaa
     2041 cttttattga aaatatcttc ttcgtttacc gaaaacaggt tttttagata acaaaaaggc
     2101 tgtgacaaaa aatgtcacag ccttttttct tttattctat ttttctttcc gtggactcta
     2161 ctttgaaata atcgatatgt gcatgctggt attctaatac cgtgaaacgg aatgatgcaa
     2221 tctcaatgat tgttcctgct tttatttctt tatatcggct agaagcaaag cccgcaagtg
     2281 tagtaaaatt cgtttcttca aactctggaa tctctacatg aaaataacgc tggaaatcat
     2341 ctaatgttag tttgccggag atgatataag agccatctga ctgttccgct atcaatgctt
     2401 cgtccaaatc tgtctcatct cggatttccc cgacgatttc ttctaaaata tcttcaatcg
     2461 tcactaagcc gcttgtccct ccgtattcat cttttaagat agcaatatgc tttccctttt
     2521 ttcgcatgat cgtcaataat ttttttatag ggatcgtttc agttgttacg atcggctctt
     2581 caatcaactg ttctactgtc agctggtcat ctttataaga ttgcttgatc aaatctggca
     2641 aggtgacata acctaaaaca ttgtcttttg attctaagat aaccggatag cgagtatgtc
     2701 cttgttcaat cgaagctttt atcgcttctt tgacggtttc tgaagcttcg aatacttgca
     2761 tggacgtacg gtctacttgt atatcttttg ccaaggtatc atcaaattca aacacgtttt
     2821 ccaaataatg atattcttct ttggtcaact ctcctttttc ataagaattc aatgcgacta
     2881 atgtcagctc ttcttgagac aatgtctctt ctccttcaga tacgagacgc acacctaata
     2941 gcttccctag ccctgcagct gaatgattca atacccaaat aaaaggaaac attactttat
     3001 aaaaataatg aagtggttta actacgaaca agacgacctt ctccgtattg acgatactat
     3061 agctcttagg aagcagttcg ccaataacca catcgatata cgtaactaaa ataaatccca
     3121 gcgccacaga gattgtactg ctaatagccg aactaaaagg tagtttaccg atcactggtt
     3181 ctagtaaagc cgcgacagat ccttcaccga cccacccgat gatcaaaccg gttagcgtaa
     3241 ttcctaattg actagctgat aaatagttat ctaaatggtg tgtaacatga agagctagct
     3301 tggcgttctt tacattttcc ttttctagtt gttccaatct tgatcgtctg attttcacta
     3361 aagcaaattc acttgctaca aaaaatgaag taattgcaat catcaaaaat aaaataatga
     3421 aattgataac catattctcc cgcctttctc tttttctatt gtaccctttt tctacaaaaa
     3481 gattgaaaat aatgcgtatt catatttagg aataaaaaag gagatgcggc atttttcagc
     3541 cacatctcct tatcacttca aaatcattgc gttacttgtt gctaaacagc tttttcatga
     3601 cctctgataa acggtgttca agtagctgac attcgacatt agttcaggtc aaagcgctac
     3661 tttcacaacc gctctttcaa ccatgttttc cgagtatttc caatgataag cggaacagtt
     3721 tcatgtttta cttcgctgta ctctagccca aaccatgttt ctggcgaggt agcgattttt
     3781 ttgattgcca gttttgcctg catgatttga cgttgccatt ttcctagagt tgtcacattg
     3841 gaaactactt catcaatcag gacaaattgg aaatcgccaa cttctcgccc tggtgtcaga
     3901 gagtatcttt gtggctgttt tggcaaacgg ccttgtttca tcaaatcatg gatgacttgg
     3961 cgaacatata cgttgatttc ctgttggacc ctgaaaccta agaacagatt cacttggaca
     4021 ataaaatcag tgtccatcat atctacttga tattctttcg tatatggctc atccgtcact
     4081 tccacatgaa caaaccaata gactcgtgct cttttaggcc gtttatctaa aatcgaatag
     4141 atgaactgtc ggccaacttt atcttcagcc atatcaggaa ccaagaagac cacatttgtc
     4201 tgatagattg gtaaagaagt atcatcacgt aattctctaa gctgcgggat ataatctctc
     4261 aaagacacat cctcggttga cgattcgcga ataatgttcc cttgttccca aataaacatc
     4321 acgactaaga tgatcaaacc aatcagaacg gctacaaaac cgcctcggaa gaatttgaca
     4381 acacttgaga taaagaaaac gacttctatc gcaccgaaga aaaaggtaac taaatatgca
     4441 agccaacgag aatgcccatt ttggataaga taaaagtaaa gcagtgctgt tgtcatcaac
     4501 atcgtcacag tgatcgatag accgtaagca gcttccatat gatgagacgt tcggaatgtg
     4561 acaaccacta gagaacaggc gatccataaa atcagattga ctgctgggat atacatttgc
     4621 ccgatactgt tcccaggata catgatcttt aaccgaggca gcagtttcaa cttgattgct
     4681 tctgaaacta gcgtaaacga acctgtaatc aatgcttgcg aagcaatgac tgcagcgact
     4741 gttgcaaaag caacgccgat cacgatccaa ctttgaggca tcatttggaa gaatggattc
     4801 aggttcttga tttgctgtgc ttcagggttt tgataaacat tcaacaacca cgcagcctgc
     4861 ccaaaataat tgataaccaa acaaattttg acgtaaggcc agctcagacg aatatttcgt
     4921 ttgccgacat gtccaagatc ggaataaagc gcctctgcac ctgtcgtagc caggaaaata
     4981 tttccaagaa tgaacaagcc taatttgtta tctgcactaa aaagcagctg gatagcataa
     5041 taaggattca gtgcacggat aactgtccag tcttgactga agttcattaa tcccattacg
     5101 cctaaaaaag taaaccaagc gaacatgatt ggtccgaatg cttttccgac aatatctgta
     5161 ccaaagcgtt ggaccatgaa taaaacaaaa ataataacta aagtgataat gacgatcacg
     5221 ttctggttat taccgaaccg gtcaaaaaac tgcggaattc ctcgcaagcc ttcaatcgca
     5281 gtcgttaccg tcacagcagg ggtcaatacc ccatctgcta gtaaggcagc tcctccgatc
     5341 attgcaggaa tgatcaaata tttcccacct tttcggacaa gtgtgtagag tgagaaaatt
     5401 ccaccttcac catggttatc tgcattcaat gcaatcacga catatttgat tgttgtcagt
     5461 agagtcaatg tccaaaaaat caatgagact gatccgataa caaaatcagg attgattcct
     5521 gacaatccgc cattatcttc aacaatcgat ttcataacgt aaagcggact tgtcccgatg
     5581 tctccgtaga cgactcccat cgctacaagc aggccagcca ttgatatctt tttggtattg
     5641 tgcgttgttg ccacgttttt ccctcatttc ttgttatatg gatatccttt aggtaagctt
     5701 gaatgataga tgatattctt atccatgaca cccgctttcc taaattgctt ctaattatcc
     5761 aagtaacaca ctgaagtgtc aacttatttg aataaaatta ttctttcttt ggacgttctc
     5821 tgatacgtct tgccacaaat gaataaagag caaggcaaaa aattgcaccc aataatgctg
     5881 gtatcaaata aaaaccgccg agttccggtc ccagtggtcc tagtaaaaat tcgccgagcc
     5941 aactacctaa aaaaccgata atgatatttc cagatacgcc tcccgggact tcctttccta
     6001 aaattgcgcc agctatcaaa cctaatatac cgccgataaa taacgaccat aaaaaattca
     6061 gcatctcctt tccctccttt atccatattt ctcactttta ttttacgctt tattcagaag
     6121 gactccaaaa atatgcgcca agaaactcca gaaaaaaaga agttgtaaca aaagtgttca
     6181 gcttatttcc gtagaagctg cttctggaac accgtgaatc agaggttgtg aaatcagcgg
     6241 tttgacctga gtattagaga aacaaacgga aaaatagttc ctcatatttt gccgtttttt
     6301 gagctaatat cgagggacag ctgattgaac atcgtttatt ggagaggttg taaaaaagct
     6361 gttctgcatc aagtaataag aacagccaag taaaaacagc tcctaatgtt tttaacttgg
     6421 ctggacttat taccgcagat gcaagctttt gaacaccgtt tattcgaaaa aaaaggactg
     6481 tgacacggct tttgtcacag tccccttttt tcgaataatt attgtccccc tgtcaccttc
     6541 acgatcacaa gtacattatt cctatgaatt aaattatttt tcttcatttt tcgttgaacc
     6601 atcttcgttc aacattgctt ttcttgacag atttacgcgt ccttgtttat cgatttcagt
     6661 tactttcaca aggatctcat cacctagttt aacaacatct tcgactttat tgacacgatc
     6721 gtttgctaat tgggaaatat ggacaagtcc atcttttcct ttgatcaagt tgacgaatgc
     6781 accgaatttt tcgatacgga caactttccc tagataaact tcaccaactt tgacttcttt
     6841 ggtcaaatct tcgatgatct tgatagcttt tttgatcatt tctgcatcag ctgaagcaat
     6901 acttactttt ccatcttgat cgatatcgat tttcacacca gtttcatcga tgattccgtt
     6961 gatcgtatcg ccgcctttac cgataacatc tttgatttta gcaggttcga tctggatcat
     7021 ttcgattttt ggtgcatatt ggcttaattc ttcacgtgga gcagcaatcg ttgaagtcaa
     7081 ttcttctaag atttccatac gtgcttgttt tgcttgagtt aatgcttcag ttaggatttg
     7141 ttctgtgatt ccttgaatct tgatatccat ctgtaaagca gtgataccat cttttgtacc
     7201 ggcaacttta aagtccatat cgccaagatg atcttccagc ccttggatat cagttaggat
     7261 cgtatagttt tcaccatcag aaacaagtcc catcgcaata ccggcgactg gcgctttgat
     7321 tggtacacct gcatccatca atgctaatgt gccagcacag atacttgctt gggaagaaga
     7381 accatttgat tccaatactt ctgctactaa acggatcgta taaggaaatt cttcttctga
     7441 agggattact tgtgctaacg cacgttcacc taatgcgcca tgtccgattt cccgacgccc
     7501 tggagagcct gcacgtcctg tagaaccaac agaaaattga gggaaattgt agtggtggat
     7561 gaatcgtttg ctgacttcca cccctaaacc atcaatgatt tggtgctctc ctagaggtgc
     7621 gagtgtacaa gctgataaag cttgtgtttg tcctcgtgtg aacaaacctg atccatgtac
     7681 acgtggcaat aagctgacct ctgatgacaa tggacggatt tcatccaatt tacgcccgtc
     7741 aggacggatc ttatcgatgg tgatcaattc acgtacgaca tctttttcaa gatcttctgt
     7801 gatttgttta acttctttca acaagtgatc ttcatcttca tgacctgcaa atttctcagc
     7861 ataagcttcg cggatatcga tcttcacctg ctcgatatta tcttcgcgag caagtttttc
     7921 ttcagtcatt actgctgctt tcattgtagc gtaagaagca tcaaagattt cttttttcaa
     7981 tacttcatca acttgcagta aagtcacttc cattttttct ttccctactg cttgaacgat
     8041 ttcttcttgg aaagcaacta actctttgat agcgtcaaaa ccaaacagca atgcaccaag
     8101 catgtcttct tctgaaactt ctttcgcgcc gctttcaacc atattgatgg cttgtttcgt
     8161 tccggcaaca gtcaattcga tatctgtttg ttctgcttgt tctactgttg gattcaagac
     8221 gtactcgcca tttacacgac caacttctac gcctgcgatt gggccgtcaa atgggatatc
     8281 tgagattgat agtgctaaag aagaacctag cattgctgcc atagctggcg agcagtctgt
     8341 ttctacactc atcacgatgt tcgttacttg gacttcatta cggaagcctt ctgcaaacat
     8401 cgggcgaatt ggacggtcga tcaaacgggc agtcaatgtt gcttccgtac ttggacggcc
     8461 ttctcgtttg atgaaacctc cagggatttt ccctgctgca tacatttttt cttcataatt
     8521 gatcgttaat gggaaaaagt ctgtatcttt tgcttcttta gaggccacag ctgcacttaa
     8581 gactactgta tctccataac ggactaaaac ggctccgttt gcttgttttg ctagctgacc
     8641 gacttcgatt tgtaagggac ggccgcccca tgtcgttttg aatacttgtt tttctgacat
     8701 aacttctcct tttccttctc gtgaaagcaa gacgctacta aacaaatgaa aactgccgaa
     8761 agccgtcttt tgagcagctt tcatttggtt agtaggagtc catctagctt gatcacggaa
     8821 ttttacttaa ttgatatacg agtaaaaaaa gtgagattca tgtgaatccc actctttttg
     8881 actaaaatta acgacgcaag cctaaacgtt tgatcaattc gcggtaacgt tgaacgtcag
     8941 ttttacgtaa gtaagctaac aagttacggc ggtgaccaac ttttttcatt aggccgcggt
     9001 atgaatgatg gtcttttttg tgcacgcgtg catgttcatt caagtggttg atttcttcag
     9061 ttaatacagc gatttgtact tctggagaac cagtatcccc ttcatggcga gcgtattcgt
     9121 tgatgatttc attcttgcgt tcttttgaaa ttgccatttc tttcacctct ttcgattatt
     9181 cccctgcgac tgagtaaaac gttggtgatt cgcttaacca agtggcaggt catgtgccta
     9241 gagcacacag ctatacttta cctgaaaccg ggttgaaaat caagtagtta atcattaaga
     9301 aaaggtagtt ttcaaacaat cttactacta cttttccatt tttgtggtaa actataatgc
     9361 gaaaatagac ttgaaacaca gtcttttaag aaaggggtac atattccatg attacaatgg
     9421 atgatatcat tcgtgaagga aatccgacct tacgcgaagt tgcaaaagaa gtttctcttc
     9481 cattaagcga agaagatatc tctttaggaa aagagatgct tgagttctta aaaaacagcc
     9541 aagatccaat caaagctgaa gaactacact tacgaggcgg tgttggctta gctgctcctc
     9601 aattagatat ttctaaaaga atcattgctg ttcacgtacc aagtcctgac ccagaggcag
     9661 atggaccttc tctaagtact gttatgtata atcctaaaat tttgagccat tctgttcaag
     9721 atgcttgttt aggggaaggt gaaggctgtt tatctgttga tcgagaagta ccaggatatg
     9781 ttgttcgcca tgccaaaatc actgtgtctt attatgatat gaatggtgaa aaacacaaga
     9841 ttcgtttgaa gaattatgaa tcgatcgtcg ttcaacatga aatcgaccat atcaacggtg
     9901 tgatgttcta tgaccacatc aacgatcaaa atccatttgc actaaaagaa ggcgtattgg
     9961 tgatcgagta gagaattggc aggttccttc gatactatgc tacaagaaag ctttataaaa
    10021 aaggggagtg ctcaaaagct gcacttccct tttttatgtt tctccactaa tcgaaattta
    10081 gatagacatc tagaaatcgc aattccaaaa aattctgtgt attggatacg catcttttct
    10141 tattttttct tatttcttcc atatgggtgt ttatctcaga gatgatagaa tagtttctag
    10201 gataaaaagt agcagctacg atgttatcct tcgtagagga ttcctgcttc agctagtttt
    10261 tgtttgatgt ttttcagcat atgcttttct gggtagcgga tcgtatgcag atggatgccg
    10321 cctgttaatt cggataacaa agtagcatta cttttttgat aacgtctcat aaaggaatcg
    10381 atatcttgtg gcgttttgat gtgcaaagtt cctgctaact caccgtaaag gggatgttcc
    10441 acaattacat caatgatttc accaccattt tcaacgatta gtctcagttc ttcttctgtt
    10501 tgatcttttc catgctggac agcgatttta ctgatgtgcc cagtattggt ttcttctgtt
    10561 tcgaggatat aacctcttgc tgttgccacg atacttttac ctgcagctcg tagtaaagca
    10621 atatcgccta caatgatctg acggctgact ccaaaagttt tcgcaaactt gctagcactc
    10681 actggttttt ctgtatttgc caaggtttgc atgatttgtt ttcgccgtgt ctctccttcg
    10741 atcacactta ttcctccttc attttgtttt ggtattcgcg cgagtgtttc atcaaatcaa
    10801 catacgtgtc tgttattgtt tccttatatc ccttattttt cagcagacta acaactttat
    10861 ccaatacttg ctgctctctt tttgcatcat aaactgcttg gttttcttct tttttcactt
    10921 gtcctatttt agttaccgta tccattcttt tttctaataa agcaaccaac tctcgatcga
    10981 tcatatcgat cttatgacgt aatgtttcaa gagactcttc tgttagttct tcctctactg
    11041 tcttgatgat acgagcagga tttcctgcaa tcactacatt gtcgggaaaa gatttggtca
    11101 caaccgaacc tgcgccgact actacgttgt cccctagagt gacacccggc gtgacgataa
    11161 ctcctccgcc taaccaaaca ttattaccga ttttaatggg ttttgcgtac tccagaccgc
    11221 tgttacgttt cactggatgt agtggatgcg tcgccgtgta aagctggaca ttcggagcga
    11281 acatgcagtt atcaccgatt tcaatcgtgc tgacatctaa aaatgtacaa ttgaaatttg
    11341 cataaaaatt ctccccaaca aatatattat atccgtaatc aaaattgata actggctcca
    11401 tatagatttt tttccctgta cggccaaaag tctctttcaa caattgggat cgcagttccg
    11461 ctgattctgc ttggttgatg atttggcttt gacttcttgc aaacatacgg tcggcaacca
    11521 gctcaggatc attggcaaaa taaagctcgc ctgcaatcat tttttctttt tcggttttga
    11581 attcagtcat aatctgctcc taattcttgt atttttttga aaacaaccgc tccgtcttct
    11641 tgaataaacg tataaggctc cgctaaataa acaggcgttt gattgattac taagattttg
    11701 gctgacggct ggcgaaactg taccagatca caaaacggat gaacttgaaa actcgttcca
    11761 acaatgacga tcagctctgc ttggctgacg gcataagccg cctgctccat gactccatct
    11821 tgaaaccctt caccatataa cacgatgtct gggcgtattt gtcctccgca tgcttgatgt
    11881 ttgtcgctgt gaagatattc tttccagtct accgtttgtt gacattttcg acaatgacat
    11941 tgatacagac tcccatgaaa attgacgagt tgcgggcttt tagcctttgc gtgcaaaccg
    12001 tcgatgtttt gggaaacaac ccaaacattt ttttctttcg ctagatgtgc tatttccaaa
    12061 tgaatgatat tgggttttgc ttcaggatga taaatctttt tgacaaattg atagaattta
    12121 tccggttcat tcaccattgc ttgatgactc agcaagtatt ccggctgctc catcccgtgg
    12181 tagattcctg acaaagatcg atagtctggc actccagaag gtgtggatac ccctgcaccg
    12241 gtcaaaaatg taatttgctg ataagctaat atctcttttg ctgcttcgtc tactgttaat
    12301 tgtttcatgt tttgtcacct cactttccat tatagcccat actcgaacaa atttattgtt
    12361 gaaaagtcag aaaaatggga ataaaaaagt gttatgggca tttgagcaat ttatcacaat
    12421 gttcactgac aaaagaatga aaacgtgtta caatactgct gtataaaagt gaggtgaatc
    12481 gatatgttac cattctttat ttattggggc gttattctgg cttgtatcgc ctggttagcg
    12541 ttaagcgttt atttctctgt tttttatctt gtacgcaaag aaaatggaaa tctttgggct
    12601 tttgctttat tcaacgtgat tgccgcaatt gttttagcca ttacattagc cgtatatcga
    12661 acatggggct ggggaatcac ccaatactca agtttgattt atctaatatt agccatttat
    12721 ggcgtcgttg ttcttttgca agcgatccta ggacgtgagc caaagaaagc ggcagcttaa
    12781 atgatcgaga tggaactgga cacaggaaat tttctgttct agttcttttt ttattgtatt
    12841 acatacattt ttgtgtataa ttatactatc gtttgcaatg gatagatgat tcacattttg
    12901 attgtttttt cggtaaaagt aaagatttct atatttatca acccgtggtg ctagttaatt
    12961 ttttc
//
LOCUS       DAZUMK010000075        12791 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000075 DAZUMK010000000
VERSION     DAZUMK010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.077"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1177
                     /locus_tag="ACG808_001933"
     CDS             <1..1177
                     /locus_tag="ACG808_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS5047554.1"
                     /translation="TPRACRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVTR
                     LKKQRYTCKNCRTHWTAQSYFVRLSHSIADHVRYKIASLLTEKVSLSFIAKSCQVSLT
                     TVTRTLKEFKSYLPKPSKKILPRVLMVDEFRSHASIEDKMSFICADGETGNLIAVLPT
                     RKLPRLTSYFLGCTNPEEVEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAL
                     NELRIREMNELRKAGQKSQAEKLKKNWRFLLKNRASINHYEYKTWKSFRAPKYPFLTE
                     AMMIDRLLEFSAPLKEAYPFFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNL
                     LTYEEGITSAMIYPYSNGKIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLINVR"
     gene            complement(1356..1685)
                     /locus_tag="ACG808_001934"
     CDS             complement(1356..1685)
                     /locus_tag="ACG808_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS5047555.1"
                     /translation="MNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSKYH
                     TKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQRL
                     EERMEEL"
     gene            complement(1675..1938)
                     /locus_tag="ACG808_001935"
     CDS             complement(1675..1938)
                     /locus_tag="ACG808_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFS5047556.1"
                     /translation="MAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQGKI
                     PFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2398..3201)
                     /locus_tag="ACG808_001936"
     CDS             complement(2398..3201)
                     /locus_tag="ACG808_001936"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS5047557.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3179..4303)
                     /locus_tag="ACG808_001937"
     CDS             complement(3179..4303)
                     /locus_tag="ACG808_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5047558.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4319..5134)
                     /locus_tag="ACG808_001938"
     CDS             complement(4319..5134)
                     /locus_tag="ACG808_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5047559.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5124..5996)
                     /locus_tag="ACG808_001939"
     CDS             complement(5124..5996)
                     /locus_tag="ACG808_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5047560.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6081..7370)
                     /locus_tag="ACG808_001940"
     CDS             complement(6081..7370)
                     /locus_tag="ACG808_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5047561.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7514..8047)
                     /locus_tag="ACG808_001941"
     CDS             complement(7514..8047)
                     /locus_tag="ACG808_001941"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047562.1"
                     /translation="MANEKATMTINCDLKEEGLEMAVFSLAQTYQIWPQIVLSGYVQL
                     SWAAIWGKHIFYNIENQFEGKKLGDITEADLIDTLQKLAYQSVQIVNIHEGWLTKLAR
                     EVGQQLGLTFSVWTVNELETIEYLARCGVYNITSRCAWAYLWKKGAQNKLFYNQNRGV
                     KRFGDGIHDHMGALFLA"
     gene            complement(8093..8242)
                     /locus_tag="ACG808_001942"
     CDS             complement(8093..8242)
                     /locus_tag="ACG808_001942"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFS5047563.1"
                     /translation="MTMITAHSGSDNTEENSWAFLRFCLDRQLCFEVDVRQQTDGTLI
                     LAHDR"
     gene            complement(8719..10215)
                     /locus_tag="ACG808_001943"
     CDS             complement(8719..10215)
                     /locus_tag="ACG808_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISEfa7 family transposase"
                     /protein_id="HFS5047564.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYS
                     FEEKIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANA
                     NKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGEREKQFAYSAHTSCDDNGFILSTIITPGNIHDSQVAFQLVKQSKRLFPEIN
                     CVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGKKGLLSKNEFLYDEYFDCYICPQDQ
                     MLAFSTVTREGYREYKSNPKECVNCPLLNQCTISKNHQRVITRHVWGDLMDEVEHLRL
                     TDLNKSIYKKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLVFAAMNLKKLATW
                     LWKGKEPLFFCSKIRNEVDKKLFQARVTSLEQLLSTVWLSVRTAFFVG"
     gene            complement(10187..11386)
                     /locus_tag="ACG808_001944"
     CDS             complement(10187..11386)
                     /locus_tag="ACG808_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFS5047565.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVRGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDDVYSLFFYNRFMKTNA
                     RSDSYDV"
     gene            complement(11516..>12791)
                     /locus_tag="ACG808_001945"
     CDS             complement(11516..>12791)
                     /locus_tag="ACG808_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5047566.1"
                     /translation="SIKDATDRASKETFANITNSFSMEINRQVNPGTPRGGGNVKGED
                     IKKISQTDSIDSYVKRINSVADLVDYDIIETQETLANQSPERAKNFKRTVMLTGVNDS
                     TKETKFVSEAYKLVEGKHLENEDKNKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEK
                     GADETVEVEIKGLFDGHNSGGVSAAQELYENTLITDIHTAAKVYGNTEDTAVYQDATF
                     FVKGDKNLDSVIKDLGKLDINWREYNLIKSSSNYPALQQSISGIYSISNKLFFGSLIF
                     AGVVVSLLLFLWMNARKKEIAVLLSLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQY
                     TADKLGNNILNKVTGDIAKQIAKQSASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYV
                     VIFMSLVLLVSLIISSFNILRRNPKELLIDNN"
BASE COUNT         3908 a         2661 c         2044 g         4178 t
ORIGIN      
        1 tactcctcgt gcttgtagaa gttgtggttc tactgtggta gatggaaacg ggaaagcaat
       61 tatagtgaaa aatgggaaga aggaaacgat tgtccgtttt gaacaataca atcatatgcc
      121 tttggttacg cgcctaaaaa agcaacgcta tacctgtaaa aactgtcgta cccattggac
      181 ggctcaaagt tattttgtcc gactcagcca ttcaatcgca gatcatgttc gatataaaat
      241 tgcttcttta ctgactgaaa aagtatcttt gtcttttatt gcgaaaagtt gtcaggtatc
      301 tttgactacc gttactcgta cattgaaaga gtttaaaagc tatttaccaa agccttctaa
      361 gaagattctc cccagagtat tgatggttga tgaatttcgt tcgcacgctt ccatagaaga
      421 taaaatgagc tttatttgcg cagatggtga aacaggaaac ttaattgctg tcttgcctac
      481 gcgtaaatta cctcgattaa caagctattt cttaggctgc accaatccag aagaagtaga
      541 attcttagtc acagacatga acgccgctta cttccagctt accaaacgtg ttctgccaaa
      601 tgcgaaagta gtgattgatc ggtttcatat tgtcaaacac atgaatcagg cactcaatga
      661 gttgcgtatc cgtgaaatga atgagcttcg taaagcagga cagaaaagtc aggcagaaaa
      721 actgaaaaag aactggcgct ttctgctaaa aaatcgtgca agcatcaacc attatgagta
      781 caaaacatgg aaaagtttcc gagcaccaaa atacccattt ctcactgaag caatgatgat
      841 tgatcgattg cttgagtttt ctgcgcctct aaaggaggcg tatccctttt ttcatgaatt
      901 agtcgaagcc tttcgagaca aagaccctga cttatttttc tccttattgg cagaacttcc
      961 cgaaacgtta gatgacggct ttcgggagaa gcttcaaaac cttctgacct atgaagaagg
     1021 tatcaccagc gcaatgatct atccttattc caatggaaaa attgaagcga agaataccca
     1081 cataaagaca atgaaacgag tatcctacgg atttaaatca ttcgagaaca tgagaattag
     1141 aatctttttg atcaatcaat taatcaatgt aagataacaa aaaatctgaa ccagaatgtg
     1201 gttcattctg acccggattt gattttgcac aactcatcag tccttattga caaagagcca
     1261 aaaatctgag ccagaatgag gttcattctg acccagattt gattttgtac aacccatcag
     1321 tccttattga caaagagccc ttctttttag tattattata attcttccat gcgttcttcc
     1381 agtcgctgta aatcgcttgg acttaattcc ccaatttttt tccatttgac ctttttgttg
     1441 tttttgggta attcgtttag agttcctacg tcaatccaag attctttttt tagtcctgcc
     1501 tgtttccaat cccttatttc aacgtatttc agctttattt tatcggattt agtatgatat
     1561 tttgaagtaa ttgacagaaa cgaaattgtg tccagtccat tttttagaat atagacaggc
     1621 cggcgtttac cgccctctgc agctgtgaaa gcaacgtaga cggttgctat atcattagta
     1681 ttcatcttca ttgaaccatt cttcaatttt ttcgtcagtg tctagttttt ggacaggcaa
     1741 tttttccgct aatctttgaa tattatggat tcgtttactt ttttccgaca attcaataga
     1801 aaatgggatt tttccttgct caacaatttg cttataaaaa acggtgacgg ctgttttggg
     1861 gctcatgcct agttcatcaa taatgctttc tgcttcattt ttcaattttt cgtccataga
     1921 aatttgtaac ttagccattt tctccacctc tttctgacaa acacattata tcacggaaag
     1981 agttcaaatg gaattaatac aactactttt gaactccttg aaatgcagtg tatttatcta
     2041 aatataagga aaggatatag tgctaaatgg ccttgataga tagaaaagtt tcaaaatccg
     2101 ccacagcgat aattgaagcg aacactcaac gacacacaga acaggaaaag actcctaaaa
     2161 gaaccaaatt aactaggacg tgtctgaaaa ctccacaaag atgtggtatg ctctggtaaa
     2221 aaacgaatgg cacaaccacg aagaccgata tggttcttgg aaaactttct ccaccctttt
     2281 tgtaattgga gggatagtgg tttattgata gcgattttcc aagttcttca agtggaacca
     2341 gacattgagc accacggcta aaaagtgaat gaagtcttta tttttaatca ataattttta
     2401 atgattacca gtaattggtt ttttcatgcg ccctatttct atttgatgtg gagttaaata
     2461 atgctgataa gtaaaaattt ttccgtcaat taaactgcca ctagtgaatc ctgttctatt
     2521 ggtaaaaaga gaatagtctg ggtattcatc tacaccaaat gataaagatt cagccataaa
     2581 ctgtttttta ccagcaaatt cagcactgct accttgatgg atatgtccta caaaaatacc
     2641 taaaatatca ctttcggcaa tcaattcagc aaaaccttcc ggaacttcag ttacaataga
     2701 tggatcttcc cacgacaatg gatgatgaac caataaaatc gttccctttt cagcaggtgt
     2761 acccaaccac tcttgtaatt gaatcatctg tcctatcgat atttttcctt cgtgctgggt
     2821 atcttgagca gaatccaagg aaataattcg taacccattt tgattcgtac aatggatata
     2881 ctctcccgtt tcattgcttt caacactcat tccttcaaag aaaacactgc gacgatcatg
     2941 attcccacga caaaaatagt aaggtgtggt tgggaaatat tgatccaaca ttttccgtaa
     3001 gacttgataa tcagaaatcc ccccttcatg tactaaatca cctgttaata gaataaaatc
     3061 gtacactgtt aacgagagtt cttgtaacaa tttttccaat gccaactgtg gatttttaat
     3121 tgttggaaat agttcagcag taaacaagtt agtttgatag ttttctctaa aatggatatc
     3181 agagagatgc aaaaagcgca tggctattcc ctctttcttc aataaaataa tcaggatatc
     3241 ctaaattttc ttgcgtctga taatcaaaaa aatgcattct ttcaggttct atgcggagat
     3301 tgactgcatc accaatctgc caagtaggtt tttcattaag gacaatcatt tctataccat
     3361 cgactttaac ggtgtacgca taactttgac ctaatagctc acgatacttc acttgaccgt
     3421 gtaaatcgct tgtttctttt gaaaaggtta cgtgttctgg tcttatgcct aaatacaatg
     3481 agccactaac ctgaagaagt tgtttttgcc aagctggatt caatgttaaa aattgactgg
     3541 ctacctttaa tgttccttga tcataagtaa ctggcataat attcatgcct ggcgaaccaa
     3601 taaaatttgc agtaaaagta tttttaggtt tcgtataaac atttttcggc gtatcaatca
     3661 tctgaattct accttcattc aataaaacaa tccggtcagc taacgtcatc gcttcaattt
     3721 gatcatgagt gacgtagaca aaagtttgtt tataacgttg atgaattttt accaattctt
     3781 ttcgtgcatc caatcgtaaa cgcacatcca aatttgataa cggttcatct aataaaaaat
     3841 aattactccg tttaaccacc gcacgagcca atgctacacg ctgccgttgc cctcctgaca
     3901 aatctttagg aagtcgatct tggtatggcg ttaaacctaa catcttcaaa gcttcttcca
     3961 aacggtgctg aatgtcagct gtcttaattt tattcgccct taatgcaaaa ataatattct
     4021 cctttacagt catgtgagga tataaagcat aattttgaaa aaccattgcg atctctcgtt
     4081 ctccacaagg tagcttattt gctagttgtg tacctaaaaa aagctctcca ctcgtaattt
     4141 tttccaaacc agcaatcatt ctcagcaaag tagattttcc tgaaccagat ggtcccagta
     4201 acacaattcg ttctccagcg gccacagcta aatcaatttc ttttaaaatg gtatgttctc
     4261 catatttttt tccgatattt cttaattcaa gcgtttctcc catgatttgt cctccaattt
     4321 atttaatacc tgcagaaaca aaagtcccga taatatattt ttgaaaaatt gcatacaaga
     4381 ctaacggtac aaacattgta attaaactaa tcgccattgc tacggtaaaa tttgtcccac
     4441 cttcagaact aatataggtc tgcatcgcca acggcaaagt ataattatct actgtccgta
     4501 aaattaatac cggccaaaca tactcattcc aagaggtaat aaaaaaccaa ataccggtag
     4561 aagtaatcgc atgtctagta agaggaaaaa caattttact catgacggtt gtttgtttga
     4621 tgttatccaa tgccgccaca tctagaaggc tcactggaat ttcccgcatt gtttgcgtca
     4681 ataaaaaaat gcccatcgca tcagcaaatt gtggtattat cacaccccaa gtagtatcaa
     4741 ttaaattaag ttttgcaatc aacaaataat taggaatcat cgcaacagta aaaggaatga
     4801 acatcgtacc aatgatgata aagtaccaga ttttcttccc tttgattcgg caataagtaa
     4861 aactatatgc cgcaaaaaaa gctacgccta acttaacaac cgttataatt gtagcaataa
     4921 aaaaagtatt tctcgtaatt ttgataattg gaagattttc gaacaaatag caaaaatttt
     4981 ccaaggtaaa cggctgtggt atcaaactag taatcgtctg ataggcatct ttcaatggct
     5041 taaaagaatt ggccataata aacaataatg gatagataat aatgattatg atgaacataa
     5101 ataagagatg tggcaaccag cgtttaattt tcatagtaaa cccccttttc cacaaattta
     5161 aatattaccc ctaagaaaac agcaaatatt aataaagtta aaactgaaat tgctgaagat
     5221 tctccgactt tatacatttc aaaagccttt tgataagcat aatagatcac gtttgcacta
     5281 ccattattcg gtccaccacg agtgatgatc ttaatcggag taaacacata ctgcagacct
     5341 tgaacaatgg tcatcaccaa taaatatagt cctgtcgaac tactcatagg caacacaatt
     5401 gtaaaaaaaa tgcgccagat gggtacacga tccaacttag ctgcttcgat catctcttga
     5461 tcaattccca caattgctgc aaaaatgaca atgaaattat aaccaaaaac tttccaagca
     5521 gtaatacctg caatcacagc aattacccaa ccttgtaaat tcatccacat aggcagtctt
     5581 aaaccaaaat ttcccaacaa gacagcaatt ggtcccgata ccggatttaa tatccatgta
     5641 aaaaccatcc ccgccactac caaagaaatc acagctggaa taaaaaataa tggtttatac
     5701 aaattctgcc ctttttctat aactattcct aataaaaagg atgtaatata tgggaacaca
     5761 cagctcagca agacaaatag aacggtatag gacaaagtat tcttcaacac tttatgaaag
     5821 actggatcag tcagcacctt tacatagttg gcaaagccaa caaattcttt agtgggggcc
     5881 accatattcc agtcaaaaaa actcaaatag atcgtataaa tcaatggata aaaaacaaac
     5941 acgcccaaca aaataattgc tggcgccaga tacaaataac ctacatagcg cttcatttcc
     6001 gacagcctcc tcatccttat gtcaaaagca gacgaccttg tttttccttt cttacaggcg
     6061 ctgtcgtctg cttgctttgc ttatttgagt aactcattaa tttccttttg agtagctttc
     6121 atcgtttcat tgacggaatt acctgataaa attttatccc gcatatccaa caatttttgt
     6181 tcagcttcta atccagcatc tccaggaaat gaactccaac tgaccatctt atccatctga
     6241 tcaattgctg gtatcatcat ctgattttca gctaagaaag cttttaatcc attttgggcg
     6301 tctgcaacat ctttacgtgg tggtaaataa ccagtacctt ctgtccattt agctaaagat
     6361 tcaacactat aaagatattt taagaaatcc caagttgctt cctttttctt atccgtttgt
     6421 gatgtaacag ctaacattgc gccaccagca ggtagtttaa cttttttccc ttcccaactt
     6481 ggtgactcaa ctgctttagc agtaaaagta ctattttttt gaatgttgtc cctttgagca
     6541 atagtggtat agaccatcgc gacgttacca tcaataaagc tttgcacacc ttgttcccac
     6601 gtagtgtgta atgccgtctg gtcttttacc atcctttgat ataattcata agcagcgata
     6661 ccatctttag aagcaaaggt agctttgcca tcttttatta cacttgcccc attactttct
     6721 aaaattgctt gttgtgccca attatcagca ggttcttgaa tatacacgcc atactttcca
     6781 gttttttctt ttattatctt agaaaaagtt tgaacttctt cccaagtttt tggaccattt
     6841 tcatccaagc ccgctgcttt taatatatct tgatttaaat ataaaaccgg attactaatg
     6901 gagtaaggaa tacccacttg tttaccatct ttttcagaca gtgccatgat attgtctaaa
     6961 aaatgatctt taaaataatc agcattatcc ggatccactt cttctactaa agattgagga
     7021 ttagtatatt tgaaattttc tgcaaaataa tctttaaaag cccatcccac ttgtactacg
     7081 tccggtattt ctttggaggc aacagaagct tgtaaatttt gcatcaagcc tttatacata
     7141 tcaggattat attttgctac aaccttcact ttgtcttgag attgattgta tttatctact
     7201 aattcattca ctgtttttcc acctagggta tccgcattaa catgccaata ctctatcgtt
     7261 acacgaccct ccgatttttt tgttgtcttg gtattcccac taccacaagc tgccaaagag
     7321 atgacactta aaacagacac aacccctata acaagatttt tctttaacat aacattaact
     7381 tcctcctaat ttctttatgg ccatttttct aatcaagaaa tattatgaac taccaatgta
     7441 aatttctagt taattttctg taaataattc gaaatttgtg catttcgggg tacagggtca
     7501 ctggacatat ccatcaagct aagaataaag cccccatatg gtcatgtatc ccatctccaa
     7561 atctcttaac tcctctgttt tgattataaa atagtttatt ctgtgctcct ttcttccata
     7621 aataggccca tgcacaacgt gaggttatat tataaacacc acagcgcgca aggtactcaa
     7681 tcgtttctag ttcattgact gtccatacag aaaatgtcaa tcctagctgc tgccccactt
     7741 cacgggccaa cttagttaac catccttcat gaatattgac gatttgaacg ctttgatagg
     7801 ctaatttctg taaggtatcg atcaaatctg cttcggtaat atccccaagt tttttccctt
     7861 cgaactgatt ttcaatatta taaaatatat gctttcccca aatcgcagcc caagataatt
     7921 gtacatagcc actaagaaca atttgtggcc agatttgata ggtttgtgcc agcgaaaaaa
     7981 cggccatttc caatccctct tcttttaaat cacagttaat ggtcatggtt gccttctcat
     8041 tggccactgc ttgaaacacc tgctcaaggg ttaccgaatc tgggacaact tcttacctgt
     8101 catgggctag tattagcgta ccatctgttt gctgacgcac atctacttca aaacaaagct
     8161 ggcgatccaa acaaaagcga agaaatgccc agctattctc ctccgtattg tcactaccag
     8221 agtgtgcagt gatcatggtc attttgaccc ctcctttaat tccgtgattt tctcccacac
     8281 gtccactata ccgatgaagc gttaatttct tgttaatttc ttgtaaattc cctgcgtttt
     8341 ttcagtaaac tagttgtaaa tcttgcatgc tccttcctac gaactcatga atcgtcgatt
     8401 ttgcccttct agctagtttc accaattaag tagttctaca ttccccgctt ttaacctact
     8461 acaccttttt ttgttttcag ctattccttc ttcttgctga aagtccttga ggttctttct
     8521 tacaggatac catcaaggtg ttcaatggct tattcccatt gaatgaccga gacttactat
     8581 cagatttctg ctcgttgaac caagtctttg atacaggatt tcttccgtca tcttctcaca
     8641 aataccacct tgcgtttctc tagcgattgg tcgatgtgca ccctcccagt ggactgtcac
     8701 tacccctttt tgctatgcct agcccacaaa aaaagctgtc ctcacagaca gccagactgt
     8761 agacaaaagc tgttccaaac tcgtaactcg agcttggaac agctttttat ctacttcgtt
     8821 tcgaattttt gaacaaaaaa ataaaggctc cttgcctttc catagccagg tggctaattt
     8881 ctttaaattc attgcagcaa acactagcat cgtatgcgtc gcgacttttt ccaagcctcg
     8941 atacttggtc caacgcatcc catgcttttc ttttgcatcc gcaaatattc gttcaatcgt
     9001 ttgtttccgt tttttgtaaa ttgatttatt cagatctgtt aggcgaagat gttccacttc
     9061 atccatcaga tctccccaaa catgtctcgt gatgactcgt tgatgatttt tacttatggt
     9121 acattggttt agcagaggac agttcacaca ttcttttgga ttggatttgt attctcggta
     9181 cccttctcga gtaaccgttg aaaaagcgag catttgatct tgcggacaaa tataacaatc
     9241 aaaatactcg tcatagagaa actcattttt tgataatagc ccctttttcc ctttgggcct
     9301 tgaataaggc aaacaaggtc gaaggttcaa atgagtcaaa aagtgaacaa acttaggtgt
     9361 cttataccca gcatcagcga caacacaatt gatttcagga aaaagtctct tcgattgttt
     9421 gacgagctga aaggcgactt gactgtcatg aatatttcca ggagtgatga ttgtgctaag
     9481 aataaatcca ttgtcgtcgc atgaagtatg ggcagaataa gcaaattgtt tttctcgttc
     9541 gcctttgacg tagtagccgc tttccggatc agctttactt atttttcttt ctttcagctt
     9601 cacttccttt tctaagtcca aagtaaaagg gcgttttcca gcctctattc gttgacgatt
     9661 gatctcatct tctaattcat cctgataggc tttcgcttca tcatgggtca tttctttcgt
     9721 aaacttatgt ttattggcgt tagctttgat gtgggtagaa tccagataca aattatcctc
     9781 tgttacaaaa cctgctttaa ctgcttgttc taaaatatag gcaaaaatat cctcaaacac
     9841 cgtcgtttct ctaaatcggc gaacataatt ctttccaaaa gttgagaaat gggggatttt
     9901 ctcctcgaag gagtagccta aaaaccagcg gtaagcgaca ttggtatcga cttccttaat
     9961 ggtttgtcgc atggaacgga taccgaataa atactgaata aacaccaatt tgatcagaat
    10021 cactggatcg atacttgggc gacctaaatc ggagtaagtg gcttcgacga ttggataaat
    10081 aaattcaaaa tcaagcgcgg catcaatctt tcgaacgaga tgatcttgag gaactagatc
    10141 atcaatcgta agcatcgcaa attgatttct accattcgta ttgtttttag acatcatagc
    10201 tatcactcct tgcattcgtt ttcataaatc tattataaaa gaaaagactg tagacatcat
    10261 ccataaaatg gactttgtct acagtctgcg cattctcatt agagaacact cccttttttg
    10321 tgacttttct gaagctagag tgaatacttt caactgcatt agtagtgtac atgatttttc
    10381 taatggcgct gccataatca aagagttgtt caacatgaga aaagtttcgt ttgcatacat
    10441 ctactgcacc agaataatga gaccaccgat tttgaaaact gtcgaaggca gcgtgtgcag
    10501 cgtttaaaga agaagcactg tagaattttt tcatgtctcg acagacttcc ttataatcct
    10561 tacttgggag gtatcgtaac gcatttcgaa caagatgaac aatacagcgc tgcacaatga
    10621 cagaaggaaa aattgctttt gcgccttctt tgagaccgga aacaccgtcc atcgaaatga
    10681 aaaaaacatc ttctacacca cgtactttca gttcatcaaa tacttgcatc cagcgatttt
    10741 tagattctgt ttgattgagc cataggccta aaatctcctt gtttccttta agatcatagc
    10801 caagaatggt gtacacagca tattctttag cttcatagtt ttcgcgtaaa gtgacgtaca
    10861 tacaatcaac gaatagaaat gcataacact tggctaatgg ccgagcttgc cattcttcca
    10921 attcaggaag gacggcatcc gtgatatcag aaatcatttc gtgggaaata tcaaagccgt
    10981 aaatatcttc aacggttgcg gcgatatcgc attgactcat tcctcgtgca tacatggaaa
    11041 gaaccttacc ttcgatgtcg gagacatctc gttttctatt aggaatcaac tcaggttcaa
    11101 aagaagcctc ccggtctctg ggaacatcaa tgtttacttt accaaagctc gttttaagcg
    11161 tcttattgcc ataaccgttt cgtcgattgt catgtttctt tggctcttta gaatgggcgt
    11221 cataccctaa atggttattc aattctcctt gaagcatttt ttcaaataag ggaccaaaca
    11281 catcttttaa aacatcttgc atatcctcta cagattcggg ttgataagta ttcaaaatgg
    11341 attcagctaa cttctcagca tcaggatttc ttttctttct agccatcgtg taatcacctc
    11401 ttgatcttat tttagaaaaa taaaaaccgt gaagcaacca cccgcctagg attgatggtt
    11461 acttacacgg tttatattac actctcattt ttggtgctaa aaagcaccga aaattttaat
    11521 tattgtcaat taataattct tttggattcc ttcttaatat attgaatgaa gaaataatca
    11581 atgatacaag aagtactaaa ctcataaata taactacata aatcatgaat ttaggtaata
    11641 cattgatatc taaacttgat aatgtcttgt taaatccttc agcttctgcc ccaccaccta
    11701 attggctaga tgcagattgt ttagctattt gtttagctat atcaccagta accttattca
    11761 atatattatt cccaagcttg tcagctgtat attgagctaa gaaataggaa ccaacgaatg
    11821 cggggattga tataaatacc atctcaatta ggaattgacc aaaaatttct aattttgata
    11881 tacctaatga tagtaatact gctatttctt tctttctagc attcatccat aagaataata
    11941 ataatgaaac tacaactcct gcaaatatta atgagccaaa aaataattta tttgaaattg
    12001 agtaaatacc tgatatagat tgttgtaatg cagggtaatt tgatgaactt ttaatcaaat
    12061 tatattctct ccaatttata tctaatttcc caagatcttt tattacacta tcaagattct
    12121 tatcaccttt tacaaagaat gttgcatctt ggtatacagc tgtatcttcg gtattaccat
    12181 aaactttagc agctgtatga atatcagtga ttaatgtatt ttcgtatagt tcttgtgctg
    12241 cacttactcc accgctatta tgcccatcga atagaccttt aatttctact tctactgttt
    12301 catctgcacc tttctcatta tcagcatcaa ataaattaga ttttattttt atcttatctc
    12361 caactttaag gttatttttt tcagctaaat ctttatgcat taagatttta tttttatctt
    12421 cattttctaa atgctttcct tctactaatt tatatgcttc tgatacaaat tttgtttctt
    12481 ttgttgagtc gttaacccca gttaacataa ctgttctttt aaaattcttt gctctttcag
    12541 gtgattgatt cgcaagagtc tcttgagttt caataatatc ataatcaact aaatctgcaa
    12601 cactgtttat tctttttaca taggagtcta tactatctgt ttgagatatc tttttaatat
    12661 cttcaccttt tacatttccc ccacctctag gtgttcctgg atttacttgt ctatttatct
    12721 ccatagaaaa actatttgtt atattagcaa atgtttcttt tgaagctctg tccgtagcat
    12781 ctttaataga t
//
LOCUS       DAZUMK010000076        12755 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000076 DAZUMK010000000
VERSION     DAZUMK010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.078"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..2725
                     /locus_tag="ACG808_001946"
     CDS             <1..2725
                     /locus_tag="ACG808_001946"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS5047567.1"
                     /translation="LKTKILKELNDLLNSYDFPQYFPEECNAQYIAQKFQIKRNTASK
                     HLNELMEQKKVVKINGRPVLFFPVHTIERYISHSLDSFEFSSIDSFREKISKKKADHF
                     FDDLIGAKQSLKEAVQQGISAMKYPGGLPIMYFGSSGVGKSYLVEKLAEYCRQTGIIH
                     ENAPFIELNCAQYFNNPELLTSQLFGHMKGSFTGADSDHIGIIEAADGGIVFLDEIHR
                     LPPEGQEKLFIHMDKGVFRRVGDSGPWRLSHVRYIFATTEQEENFFLETFRRRIPIVC
                     TLPNYQDREISERKEIVFHLLEKESTLINKEILVSEAALSFLIRLDLYGNIGELDGLI
                     KQTIANKLFSGSNKKYIEIHISDLPSRYLLTENIELKNQIASQRFYIFSNGNYVHDCD
                     ETSTVTTNLRGLIHRVSKYYSECHDVNRNSEEFRRFVKDQMAEFNDRFIYQKNSEVLV
                     KYYLPEIQNIVQKISPSLFVHLSGNTVQAIATYLAIRDQWGYGKENASANEIEKLYEN
                     LKCNYLTDLNVFLLIFENALDTTLTIWDKLYISLCVISETGNEAKDINAIILAHGYAT
                     ASSIANVANRLVNQFVIDSIDMPLDISFSEVIDRLLHYIEYRKPKDGLVIFVDMGSLA
                     QIKTEIEQVIEVPTMIINNVTTEMAIETAQLIQSTSDIQKVVKKLPYSQFEKQVLYPI
                     KIRKRTIVVSCNTGLGTSIKIKEMMENNLSKDLGIEFLPYENETLRDTQQLEFLIKTK
                     DIIAIIGTDDPRVSNIPYISLEELFDGEIGKLYKVLLRVYGKEIAEAINQNIVKNSSL
                     NRLIDSLTILDAEKVVNQIEESLKDYQMRVGKTLSSLSKINLYVHISCMIERLIRNQG
                     VSSYPAIDQFLKRYETEVASIQKALSKIEKLYNIKIPMEEIAYVCNIIVSY"
     gene            2871..3275
                     /locus_tag="ACG808_001947"
     CDS             2871..3275
                     /locus_tag="ACG808_001947"
                     /inference="COORDINATES: protein motif:HMM:NF015567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5047568.1"
                     /translation="MSHGPLAKGMIETLSIIVGDLDNIDFRCAYVDGNNDVEMIIDDY
                     LKESMGNETIVVTDIFGGSINNEWLRRLPDLSKVYLISGMNLSLLVDLYLKFQLVSSD
                     QIEEIIDHAIQASSGSIKFCNQLDYEQTVDDF"
     gene            3293..3763
                     /locus_tag="ACG808_001948"
     CDS             3293..3763
                     /locus_tag="ACG808_001948"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5047569.1"
                     /translation="MIKLVRIDHRLIHGQVALTWTNAISADCILVANDEILNDPIQKT
                     TLKLAAPHNVKVVIKSIQDSIDAINSGKTDKYKLFVVTKTISDAKRLANGVEQIKSVN
                     IGSVKETSGSSALTHTIFVTEEERAELKGMVSEGIEVFAQQVPTDKAIDLKKSL"
     gene            3787..4569
                     /locus_tag="ACG808_001949"
     CDS             3787..4569
                     /locus_tag="ACG808_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS5047570.1"
                     /translation="MDSLLVQAILLGLLTAFIVIDWMTGTNNLSRPIVSSVFVGLIMG
                     DLKMGIMMGATLELAFIGAITIGAARPPDTVSGGILGTAFAIATGKGAEFALTLAFPI
                     AALFLLVDNLLTLLVLPMIARKADRYAEKGELKNVSKMHVLGFILVKSLPRALFVGFS
                     FYLGSPVMERILNAIPTFVQDGINIAAGIMPALGFAILLQMLLKKDVFVYFVIGFALF
                     SYLHVPILGIAIFGACLAYIIVNMETKAAMGSRGEEMVEDDF"
     gene            4559..5383
                     /locus_tag="ACG808_001950"
     CDS             4559..5383
                     /locus_tag="ACG808_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017867003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS5047571.1"
                     /translation="MTSSGKALTKDDLTKICIRSCALEASWNFERQQHMGFAFAMMPA
                     LEKIYSGNEEEKQKAAQRHLEFFNTAPPLSTFIMGIVASMEEENAKNPDDFDTSTITS
                     IKTALMGPLAGIGDSMFWGTLLVIGIGVGASLAKEGNILGPFLFLLIFNVPNFLLRYF
                     GVMQGYNFGTKLLENIESSGVMKTLTHAASILGLMVIGGMSGSMVSIKVAGSIGQGDE
                     ATKIQSIIDGILPNLLSVLCVFLIYWMLKKGFKTTYVLFGIFIFGIIAAFFGFLTT"
     gene            5418..6416
                     /locus_tag="ACG808_001951"
     CDS             5418..6416
                     /locus_tag="ACG808_001951"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017867002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS5047572.1"
                     /translation="MLNYNAKEMKKKGKRIYGIREDVEKIAKQAYDSGISNIFFTASG
                     GSVAVMQPFEYVLMTESSIPVYSMTAAELVLMGHKQLNEKSLVILYSKSGDTKETIKA
                     AEYLKERNVPTVGIVSKEDTPLEALSTYAINYLDGRPQEMPLYFLIMSIMNLNGEFPE
                     YTRFADELKNLPTALNSVREQADEKGKKYAEEFKEDPYQIWIGSGNLWGITYSYSMCV
                     LEESQWLRTKSVSSPEFFHGTLELVEKGVCVTLLKTEGETRLLDARVEKFAVQYTDKF
                     NVFDTKDYELPGISDEFRKYFCAPVMWAALGRVSVHLEDIRNHSLDKRRYYRVVEY"
     gene            6433..7287
                     /locus_tag="ACG808_001952"
     CDS             6433..7287
                     /locus_tag="ACG808_001952"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFS5047573.1"
                     /translation="MTAYVIGFGDNVVDYYENLNQKFPGGNAVNFAVNAKKSGVKAYY
                     VGSLSSDPDGVLLQHAISSEGVDISFSEVIDEPTEQAHVTIKDGDREFIGGVRGSRLT
                     PALSAELIDLMKNSSLVHTSCHSRTETKIKELKKYDVKVSFDFSDLDKYRTHEYLSDV
                     CPSIYIAQFSVATDDQEEINRLISECKDFEVPYLLFTRGSKSPVFVDTRENKTYEGFI
                     RKVESPHDTMGAGDAYFAAFASNFVLSKREKSMIEHVIDCFHKGANLAYETLMIDGSF
                     GHATPINP"
     gene            complement(7861..9111)
                     /locus_tag="ACG808_001953"
                     /pseudo
     CDS             complement(7861..9111)
                     /locus_tag="ACG808_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397514.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            9628..9819
                     /locus_tag="ACG808_001954"
     CDS             9628..9819
                     /locus_tag="ACG808_001954"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047574.1"
                     /translation="MGKYSFEFKSTIVQEYLEGEGGLGYLAKIQGVRSKKGTRMDQSK
                     VRPKNLTFGGRYLYGKISF"
     gene            9800..11211
                     /locus_tag="ACG808_001955"
                     /pseudo
     CDS             9800..11211
                     /locus_tag="ACG808_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612187.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            11286..12569
                     /locus_tag="ACG808_001956"
                     /pseudo
     CDS             11286..12569
                     /locus_tag="ACG808_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         4257 a         1999 c         2564 g         3935 t
ORIGIN      
        1 tttgaaaacg aaaattttaa aagaattaaa tgatctactt aacagctatg attttccaca
       61 atattttcca gaagaatgta acgcgcaata tattgcccaa aaatttcaaa taaaaagaaa
      121 tacggctagt aagcatctaa acgaactaat ggaacagaaa aaagttgtga aaatcaatgg
      181 tcgtcctgtt cttttttttc cagtccatac aattgaacgg tatatatcac attcattaga
      241 tagttttgag ttttcttcaa ttgattcgtt tagagaaaaa atttcaaaga aaaaagcaga
      301 tcattttttt gatgatttaa ttggtgcaaa acaaagttta aaagaagcgg ttcaacaagg
      361 gatatcagca atgaaatatc caggtggatt accaataatg tattttggat cgtccggagt
      421 tgggaagagt tacttagtgg aaaaactagc tgagtattgc cgacaaactg gtattattca
      481 tgagaatgct ccgttcatcg agttaaactg cgctcagtat tttaataatc cagagttatt
      541 aacaagtcag ttgtttggcc atatgaaggg atcgtttacc ggcgccgatt ctgaccatat
      601 tggtataata gaagcagcag acggtggaat cgtctttctt gacgaaattc atcgtttacc
      661 accagaagga caagagaaac tttttattca tatggataaa ggcgtttttc gaagagttgg
      721 agatagtggt ccttggcgtt tatcacatgt tagatatatt tttgcgacaa cggaacagga
      781 agaaaatttt tttctagaaa cttttcgacg aagaattccg attgtttgta ctttgcctaa
      841 ttaccaagat agagaaatta gcgaacgaaa agagattgtc ttccatttat tagagaaaga
      901 gtctactctg atcaataaag aaatattagt ttctgaagcc gcccttagtt tcctgattcg
      961 cttagatttg tatggaaaca ttggtgagct ggatggacta ataaaacaaa ctattgcgaa
     1021 taaactattt agtggaagca ataaaaaata tatcgaaatt catatttctg atttacctag
     1081 caggtacttg ctaacagaaa atattgagtt aaaaaaccaa attgcgagcc aacggttcta
     1141 tatctttagt aatggtaact atgttcatga ttgtgacgag acttcaactg taactacaaa
     1201 tttacgaggt ttgattcatc gtgtttctaa atattattca gagtgtcatg atgtgaatag
     1261 aaatagtgaa gagtttcgac gttttgtgaa ggaccaaatg gcagaattta atgatcgttt
     1321 tatttaccaa aaaaattcag aagtcttagt taagtattat ttaccggaaa tccaaaatat
     1381 tgttcaaaaa atctctccca gtctttttgt tcacctttct ggcaatacgg tccaagctat
     1441 agctacctat ctcgctatta gggatcaatg ggggtatggg aaagagaatg cttcagcgaa
     1501 tgaaattgaa aagttatatg aaaacctaaa gtgtaactat ttgacggatt taaatgtatt
     1561 cttactgatc ttcgagaacg ccctggatac aacattgact atttgggata aactgtatat
     1621 cagtttgtgt gttattagtg agaccggaaa tgaagcgaaa gacattaacg caattatttt
     1681 agcccatgga tatgcgacag ctagtagtat tgctaatgtt gccaatcgtt tggttaatca
     1741 gtttgtcata gattctattg atatgcctct agatatcagc tttagtgaag taattgacag
     1801 attacttcac tatatagagt atcgaaagcc taaagatgga ctagtgattt tcgttgatat
     1861 gggaagttta gctcaaatta agactgaaat tgaacaagta atcgaagttc caacaatgat
     1921 tattaataat gttacaaccg aaatggctat tgaaaccgca caactgattc aaagtacgag
     1981 tgatatccaa aaggttgtaa aaaaattgcc gtattctcag tttgaaaagc aagttctata
     2041 tcccatcaaa attagaaaac gtacaatcgt tgtttcctgc aatactggtt tgggtacctc
     2101 cataaaaatt aaagaaatga tggaaaataa tttgtcgaaa gacttgggca ttgaatttct
     2161 gccatacgaa aatgaaactc ttagagatac tcagcaatta gagtttctca ttaaaacaaa
     2221 ggatatcatt gccattatcg gaacagatga tccgcgtgtg tcgaacattc cctatatttc
     2281 tcttgaagaa ttatttgatg gtgaaattgg caaactctat aaagttcttt tgagagtcta
     2341 tggaaaagag attgctgagg caattaatca aaatattgtt aaaaatagct cattaaaccg
     2401 cttgattgac agtcttacta ttttagatgc cgaaaaagtt gtcaatcaaa ttgaggaaag
     2461 tttaaaagat tatcaaatga gggtggggaa aacgttatca tcattgtcca aaattaatct
     2521 ttatgttcat atcagctgta tgattgagcg actaattcgt aatcagggag taagtagtta
     2581 tccagcaata gatcagtttc ttaagcgata cgaaactgag gttgcttcta ttcaaaaggc
     2641 acttagtaag attgagaaac tttataatat taagattcct atggaagaga ttgcatatgt
     2701 gtgcaacatc attgtaagtt attgataaaa taaagtgaca ctattcacag cgatggaaat
     2761 agtgtcactt tattttttgt acgccctttc atttaagtgg tgacagtaat tggcacagta
     2821 tttgctaata acaaagcgaa aggagttcgg gtatgagaca aatattatgt atgagccacg
     2881 gtcctctagc aaaaggaatg attgaaactt tgagtattat cgtaggagat ttggataata
     2941 ttgattttcg atgtgcctat gtcgatggaa ataatgacgt agaaatgatc atcgatgatt
     3001 atctaaagga atctatggga aatgagacga ttgttgtaac cgacattttt ggtggaagca
     3061 ttaataatga gtggctaaga cggttgccag atctttctaa agtctatttg atttctggaa
     3121 tgaatttaag tcttttagtg gatttatatt taaaattcca attagtttca tctgatcaga
     3181 ttgaagaaat tattgatcat gcaattcaag catcttctgg ttcgattaag ttttgtaatc
     3241 agctagatta tgagcaaacg gttgatgact tttaaataaa ggagggaaga gaatgattaa
     3301 attagtgagg atcgatcatc gtttgataca cggtcaagtt gctttaactt ggacgaacgc
     3361 aatcagtgcg gattgcattt tagtagcgaa cgatgaaatt ttgaatgatc caattcaaaa
     3421 gacaactttg aaattagccg caccacataa cgtgaaggtt gttatcaaat caattcaaga
     3481 ttcgattgat gcaattaata gtgggaagac agataaatac aaactatttg tagttaccaa
     3541 aaccatttcc gatgctaaac ggttggcaaa tggggttgaa caaattaaat ctgtaaatat
     3601 cggtagtgtt aaggaaacta gtggcagtag cgctttgacg catacaattt ttgtaacaga
     3661 agaggaacga gcagaactta aaggaatggt ctcagagggc attgaagtgt tcgctcaaca
     3721 agttccaaca gataaggcaa tagatttaaa aaaatctttg taataaaaaa ataagggggt
     3781 aatttcatgg atagtctctt agtacaagca attttgcttg gtctcttgac tgcgttcatc
     3841 gtaattgact ggatgacagg tacgaataac ttatcaagac caatcgtatc ttccgtcttt
     3901 gttggtttaa ttatgggcga tcttaagatg gggattatga tgggggcaac actggagtta
     3961 gcatttatcg gagcaataac aatcggagcg gcacgtccgc ctgatacggt atctggtggg
     4021 attttaggga ctgcgtttgc tattgcaact ggcaagggag cagaatttgc gttgaccttg
     4081 gcattcccaa tcgctgcgct attcttatta gttgataatc tactaacatt gcttgttcta
     4141 ccaatgattg ctagaaaggc agatagatat gctgaaaagg gcgaactaaa aaatgtgtcg
     4201 aaaatgcatg ttttaggttt tatcttagta aaaagtttac cacgggcttt atttgtcgga
     4261 ttctcatttt acttaggaag tcctgttatg gaacgaatct taaatgctat tccaactttt
     4321 gtccaagatg ggattaacat tgctgcaggc attatgccag cactaggttt tgctatttta
     4381 ttgcaaatgc ttcttaagaa agatgtcttt gtatatttcg taattggttt tgcactcttt
     4441 agctacttac atgtgccgat tctggggatt gcaatcttcg gcgcatgtct agcttatatt
     4501 atagtaaaca tggaaaccaa agctgcaatg ggttcaagag gggaggagat ggttgaagat
     4561 gacttctagt gggaaagctt taactaaaga tgatttaaca aaaatttgta ttcggtcatg
     4621 tgcattagaa gcatcatgga attttgagcg ccaacaacat atgggctttg cttttgctat
     4681 gatgccagcg ttagaaaaaa tttatagtgg caatgaagaa gaaaagcaaa aagcagctca
     4741 aagacatcta gagtttttta acacagctcc gccattatca acattcataa tgggaattgt
     4801 ggcttctatg gaagaagaaa atgccaaaaa tcctgatgac tttgatactt ctacaatcac
     4861 ttctattaaa actgcattga tgggaccatt agctggtatt ggtgattcaa tgttttgggg
     4921 taccttatta gttatcggta tcggtgtagg tgcttcatta gcaaaagaag gcaatatttt
     4981 gggtccattt ttatttttac taatcttcaa tgtccctaat tttcttctcc gttactttgg
     5041 agttatgcag ggatataact ttggtacaaa gctattagag aacattgaat cctctggcgt
     5101 catgaaaacg ttgactcatg ctgcctcaat tttgggtctt atggtaattg ggggcatgtc
     5161 tggaagtatg gtatcaatca aagtagctgg ttctattgga caaggtgatg aagcaacaaa
     5221 aattcaaagt atcattgatg gtattttacc aaatctgctg tcagtattat gtgtatttct
     5281 gatttactgg atgcttaaaa aaggctttaa aacaacttac gtgttattcg gaatctttat
     5341 ctttggtatc atagcagcct tcttcggatt tttgacaact taattgaata atctaaataa
     5401 ttgtaaggag agaactaatg ttaaactata atgcaaaaga aatgaaaaaa aaaggcaaac
     5461 ggatttacgg tattcgtgaa gatgtagaaa aaattgcaaa gcaagcttat gattctggaa
     5521 ttagtaatat tttctttact gcttctggtg gatcggtagc cgtaatgcaa ccatttgagt
     5581 atgtattgat gactgaatca agtattcctg tttactctat gactgcagct gagcttgtct
     5641 taatgggaca taaacagttg aatgaaaaat ctttagtaat cttatactca aaatctggag
     5701 atacgaaaga aactattaag gcagctgaat atttaaaaga acgaaacgtg ccaaccgttg
     5761 gtattgtaag taaagaagat acaccgttag aggctctatc aacttatgca atcaattacc
     5821 tagacggccg ccctcaagaa atgccacttt atttcttaat tatgtctatc atgaatttga
     5881 atggggaatt tcctgaatac actcgttttg ctgatgaact gaaaaactta ccaacggctc
     5941 tgaattcagt tagagaacag gctgatgaaa aagggaagaa gtatgccgaa gaatttaaag
     6001 aagatcctta tcaaatttgg attggtagcg ggaatttatg gggaatcacc tactcttatt
     6061 caatgtgtgt gcttgaggaa tctcaatggt tgcgtacaaa gtctgtctca tcaccagagt
     6121 ttttccatgg taccctcgag ttagtcgaaa aaggagtttg tgtaacactt ttgaaaactg
     6181 agggggaaac acggctttta gatgctcgag ttgaaaaatt tgcggtccaa tatacagata
     6241 aatttaatgt ttttgatact aaggattatg agctaccagg tattagtgac gaatttcgaa
     6301 agtacttttg tgctccagtt atgtgggcag ccctaggtcg cgtcagtgtt catcttgaag
     6361 atattcggaa ccactctttg gataaacgtc gttattaccg agtagttgaa tactaaaagt
     6421 aggtgattaa ttatgacagc gtatgtgatt ggatttggcg ataatgttgt tgattactat
     6481 gagaatttga atcaaaaatt tccaggggga aatgcagtta attttgctgt gaatgcaaaa
     6541 aagagtggtg ttaaagcata ctatgtcggt agtctatcat ctgatcctga tggcgtgtta
     6601 ttacaacacg ccatcagttc tgaaggtgtt gatatctctt tctcggaagt tattgacgag
     6661 cctactgagc aagcacatgt aactattaaa gatggagata gggagtttat aggtggcgtg
     6721 cgtggctccc gattgacacc tgctctctca gcagaactta ttgatttgat gaagaacagt
     6781 tcattagttc atactagttg ccatagtcga acagaaacaa aaattaaaga attaaaaaaa
     6841 tatgatgtta aagtttcttt tgatttttct gacttagata aatatagaac gcatgaatac
     6901 ttgtcagatg tttgtccgtc aatttatatt gcacagtttt cagtcgctac agatgatcaa
     6961 gaggaaatta atcgcttaat tagtgaatgc aaggactttg aggttccgta tcttcttttt
     7021 acgcgtggta gtaagagccc tgtttttgtt gacactcgag agaataagac ttatgaagga
     7081 ttcattcgca aagttgagtc acctcatgat acaatgggcg caggtgatgc atactttgca
     7141 gcttttgcaa gtaattttgt cttatctaaa cgtgaaaaat caatgataga acatgttatt
     7201 gattgttttc acaaaggtgc aaaccttgca tatgaaacat tgatgattga tggctccttt
     7261 ggtcatgcta cacctattaa cccttagctt tgtgctttgg gcttttattg ctggaatttg
     7321 tatagtagac tcatcataac tatatttttg tatgtgaaaa ggcaagaggc tatgatgaaa
     7381 ataacactgg ctaattgcat gagggagcat atcgttgtta aagcgtcatt gatagtatga
     7441 ataaatatct cgataatatt atattgttat ttacttaatt tattgattag tcaagcaaag
     7501 ataatcttct gttacttaac gagactagta ggtacaagca cttcatgtta tgtacgaccc
     7561 aaaaatattg ctgttgatta tttgctgaaa ttattgcgag cttcaattta tttttaaagg
     7621 acttggtagg ctgcgtgatg aaataagact tggaaggctg tctaaaccaa tatatagtag
     7681 actaaatagt aaaaaagagc atggtaattg tctctttgtc aataagtcta atggtaataa
     7741 gacaagttgg agattgagaa tatttttatt gtgtaaccta ttttgaagcg tacttaacta
     7801 gccccggcca aaatcaaatt ttttcggggt atatcaaggg tttgcgtcaa taacagaatg
     7861 ttatttatct atcacgttcc ttggtggcgt gtagagttga tggttacgta gtagcgtatc
     7921 taccagacgc gtaaattttc ttgcggttaa gacgtgtgct cgtttgtgtt tatttttggg
     7981 acctctttac atttgctttg ataaaaagct ttatattgag gaagatattt ttttacagag
     8041 ttggaagctt caactaagta gtatctaaaa tagcgactcc tttgttttgt cagaggtgta
     8101 ttttgtgacc gatagttaac agattgtttg cctttccaac ataagcctgc gtattttgcc
     8161 aattttgttt gatcttcaaa tcgttcaatt tgaccgattt cagcgattaa tccagcggcg
     8221 tgtacctttc caatacctgg aacactagtc aaatattgat attctggaat aacgacaaca
     8281 aattgctcgg ttgctttatc caagtctttg atgttctttt caagcgcacg aatttcacat
     8341 acaaaaacat ttagaacgac attgatggat tcttatgcaa gactatctaa ccgataactc
     8401 acggcaattg cacgttgaat cgatttggca tttttttctg gatctttaaa gcgacatttc
     8461 ctttttcttt tagtaagtca cagaaagctt ccaaaggaag cacagccaat tcatctagcg
     8521 tgaagtcttc tgtcacaaga gtaatgacag tggcactata gagacttgtc gattcatcgc
     8581 gcatttatcg agtaagcgta ttgcatttgt acgttagatt ttccagaaag tgttgttttg
     8641 tacgagtgaa gttgcttaat gagtcgatag cgagtgcgtg ttaaacgctg taaagccacg
     8701 tacctctctt ctttgatagg agaagttgtg aaccgttgaa tacgcaataa atcagcaatt
     8761 cttaacgcgt tgtttcgatc agtcgtatct tcctcaaaca tgcgactgaa ttgtttcatc
     8821 cgaaaaggat tttcaatcct cacaagtgca ttgagttttt ttagctctgc atcttcatgg
     8881 aaaaacatcg aaggatgaaa gctgtacatg gacgtttaat tccatctcaa taacgatttg
     8941 accgaagtga ttgttttcat tgaacacaag aatcagttct cgtgtcaatg gagccctttc
     9001 aatgtcatta gcaacagaat tttcagtcaa tatagatagc tgatcgtcgt ctgttaggaa
     9061 gcaaacgtct aatttttcag aactaacgtc attaccaaca aataatttca tggaataaaa
     9121 acctattttc gatataaatt ctagaaaatg cttcgatact gtgggattcc acagaaactc
     9181 actgtgtggt cacgacctcg cctaatagaa tacggtaata aaagagctaa tgagttgata
     9241 gtaaacacag cgctttggat aacagactta tgaaggtaga caaagctaca ggactgatta
     9301 gcaaattctc gttgaatcct ttccatacat ccattgtact ctgagaaatc acacaataac
     9361 gaaataaatt ccggaaggaa tatgttattc aaaaaaattt gtgttttata gatactttaa
     9421 aagaaactta ttaggcaaaa tatattttac gaagactgat gagttgtgca aaatcaaatc
     9481 tgagtcagaa tgaacttcat tctagctcag atttttgttt aatgacttca gtcagtttct
     9541 tgcattacgt gaatgaaaat gggactggtg atgtccaaac cctaaaaagt tagaccaaaa
     9601 tctaacttca ggggggatgg tatttttatg ggaaaatata gctttgaatt caagtcaaca
     9661 atcgttcaag agtatcttga aggagaaggt ggattaggat atttagcaaa aatacaagga
     9721 gtgagatcaa aaaaaggtac acggatggat caatcaaaag ttagaccaaa aaatctaact
     9781 tttgggggtc gttatttgta tggcaaaata tccttttgaa ttaaaactaa aaatcgttaa
     9841 agaatatctt agtggcgtgg gcgaatatcg gcatcttgcc aacaaatacg gtatttcaag
     9901 caagtctcaa gtaagtgatt gggtgaatgc ctatcgtgaa tacggagagg aaggtctatt
     9961 gaaaaagcat caaaatcaga aatattctgt tcaatttaag ttaaatgcgc tagagttata
    10021 tcaaacaagt gagatgtcct atcgagaagt tgccagcact ttagaaatga acaaccctgc
    10081 gcttattgcc aattggatgc gtaatttcag agaagcaggt attgaggggc tctcaaaaga
    10141 gaaagggcga ccacttactt tgactagaaa aaaagaaaac aaaaaagaaa atacaacaac
    10201 tgaagaacgc gagcgtatca aggcattaga aaagcaagtc cgatcccttc aaattgagaa
    10261 tgccttttta aaagagttga ggaagctgcg aaaacaggaa gcccagcaaa gacgcaagaa
    10321 tcaatcgcac gaatcatcgc cagcctccga ggatcattca aactagttga gcttcttgaa
    10381 acattgaaat ttcctaaagc aacctatatg tattggcaga aacggtttga cagaccaaac
    10441 ccagaccaag aaattgaaga aaaaatgctt gaaattcgca aggagcataa agactatggc
    10501 tgtctgcgac taaaaaaaga attagagaat caagggattc atgtcaataa aaagaagatt
    10561 caacgtctga taaaaaaact aggtatcgaa gtaaagtctt acactcgaaa atctcgacgg
    10621 tacaattctt atcatgggaa ggttggcact gtcgccaaaa atcgtattca tcgtcggttt
    10681 tatacaaata tctgtcacca aaaaattaca actgatacca cagagtttaa gtattatgaa
    10741 gtagacacca caggtgtcgt tcgtcagaaa aagctgtatt tagatccttt catggatttc
    10801 tacaatagtg aaattctatc ttatcggatt tctgaaaaac caaacgcact agccataatg
    10861 gaaggattag aagaagcaat ccagatgaca aatgattgtc catttcgtag aaccttccat
    10921 tcagatcaag gatgggccta tcaaatgaat gcttataaga ataaattgaa gcagcataaa
    10981 atcttccaga gtatgtctcg taaagggaat tgtttagata actcacctat ggaaaacttc
    11041 tttggtttat taaagcaaga gatttttcat ggagaagtct atcggagttt agatgaattg
    11101 aaaacaaaaa ttgaccaata tatctattat tacaaccata aacgcatcaa aaagaaatta
    11161 aattggcgaa gtccggttca gtttcgagaa gctgtaaaaa cagctgtcta acagttaatt
    11221 ttatcgtaca aaaaaggagt ggatttctcc actccataaa agtctaactt tttggggtcg
    11281 ctacaaatgc ttatcgagaa tttggagaag aaggcctctt gcgtaagcga aataagcgaa
    11341 gttattctgt tcaattcaag atagatgcga tagagttata aaaaacgggt gagctatcct
    11401 atcgtgaagt ggcgaatcat ttagggataa accaacctgc gttagttgtc aattggatga
    11461 aaatttttgt tcaaaaggat tgcacgggct ctcaaaaatg aaagggtcct cctccatttt
    11521 gtctaagaaa gaataaaagg aagaaacaag caaactgacg cctaaagagc ataatcgtat
    11581 caaagaattg gaaaaataag tgcgtttcct tcagattgag aatacctttt taaaagaatt
    11641 gaggaagctt agaaaacagg aagtctaaca aagacgaacg aatcaatcgc acgaatcatc
    11701 gacaacctcc gaggatcatt caaattagtt gagcttcttg aaaccttgaa atttcctgaa
    11761 ttaaccttta tgtattggca aagacgattt gatcgaagaa attcagatca agaaatcgaa
    11821 gaggaaatgc ttaaaataag aagtcaatat aaagattatg gctatttacg tatgacaaag
    11881 gaattgtggg accgtgggtt ccatgttaac aagaaaaaag tacaacgttt gattcgaaag
    11941 ctgagtattg aagtacgatc atttacgaga aaatatcgcc gctataatac ttatcgtgga
    12001 agaatcggca tgattacaaa aaatcgaatt catcgacgct tttacacaag caactgtcat
    12061 caaaaggtta caacagatac gacagaacta aaatattttg aaatggataa atacggtgcc
    12121 aatcgtgaaa agaagctgta tcttgatcca tttttagata tgtacaacag tgaaatttta
    12181 ttttatcgca tttctgaaaa gccaagtgcg ttggctatca tggaagcttt agaggaagct
    12241 atctgcgcaa cggaatgtat ctatcgtcga acttttcact ccaaaaagga tggacctacc
    12301 aaatgaaagc ttatagtcat aagttgaaag aacataagat cttccaaagt atgtcccgta
    12361 aggagacctg cttagataat tcgatcattg agaacttcct tagtctatta aagcaggaaa
    12421 tttattatgg aagagactat cgtatctttg aagaattgaa gcaagccatt gatggctata
    12481 ttcattacta caaccatgag cgtatgaaaa ggaaactaaa ctggcagagt ctggtgcaat
    12541 ttagagtaat agcattcaaa gctgtctaaa atcatcaaga aaagcaaaga aaaacggagt
    12601 gaattttctc cactccgaaa acatctatct tttttaggtc actacagaat caattctgat
    12661 tcatcagacc cataaattat agatcctaat aatttatacc atgctctttt tactcatatt
    12721 tacatttatc tttgggtatt ttatggttta agttg
//
LOCUS       DAZUMK010000077        12691 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000077 DAZUMK010000000
VERSION     DAZUMK010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.079"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            97..948
                     /gene="licT"
                     /locus_tag="ACG808_001957"
     CDS             97..948
                     /gene="licT"
                     /locus_tag="ACG808_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFS5047575.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     EKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            1041..2927
                     /locus_tag="ACG808_001958"
                     /pseudo
     CDS             1041..2927
                     /locus_tag="ACG808_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
     gene            complement(2967..3869)
                     /locus_tag="ACG808_001959"
     CDS             complement(2967..3869)
                     /locus_tag="ACG808_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS5047576.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            4100..5503
                     /locus_tag="ACG808_001960"
     CDS             4100..5503
                     /locus_tag="ACG808_001960"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFS5047577.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            5503..6936
                     /locus_tag="ACG808_001961"
     CDS             5503..6936
                     /locus_tag="ACG808_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFS5047578.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            7081..8007
                     /gene="trxB"
                     /locus_tag="ACG808_001962"
     CDS             7081..8007
                     /gene="trxB"
                     /locus_tag="ACG808_001962"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFS5047579.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            8221..9948
                     /locus_tag="ACG808_001963"
     CDS             8221..9948
                     /locus_tag="ACG808_001963"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFS5047580.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            9963..10313
                     /locus_tag="ACG808_001964"
     CDS             9963..10313
                     /locus_tag="ACG808_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFS5047581.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(10405..11265)
                     /locus_tag="ACG808_001965"
     CDS             complement(10405..11265)
                     /locus_tag="ACG808_001965"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFS5047582.1"
                     /translation="MTSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            11357..11785
                     /locus_tag="ACG808_001966"
     CDS             11357..11785
                     /locus_tag="ACG808_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFS5047583.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGKIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            11947..12123
                     /gene="rpsU"
                     /locus_tag="ACG808_001967"
     CDS             11947..12123
                     /gene="rpsU"
                     /locus_tag="ACG808_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFS5047584.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            12150..12596
                     /locus_tag="ACG808_001968"
     CDS             12150..12596
                     /locus_tag="ACG808_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFS5047585.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         4164 a         2159 c         2795 g         3573 t
ORIGIN      
        1 taaacctgat gagtctccca tggtttcatg gacggctttt tgggtttttc tttttgtttt
       61 ggatacagcc ctatacatca tggaaaggag caaaacatgc tgattgagaa agtactgaat
      121 aacaacgtcg taataaccag ggatgaaaga aatcaggaaa tgatcgtgat gggaaaaggt
      181 ttggcattca aaaagaaagt gggtgacccg atttttgact catgcatcga aaaaaaattt
      241 cagttgacaa atgacgggct ttcacaacag ttccaagaat tggcacaagc gattcctctt
      301 gagtacttag aaatttcttg tgaaatcatt gaatatacaa aagaggctct acaagtccag
      361 ttgaatgatt ctgtgtatat tgcattgacg gatcatttgt atacggctat cgaacgtcac
      421 aaaatgggaa tatttgtacc aaatgtattg ctgtatgata tcaaacaact atttcctaaa
      481 gagtatgcca tcgggaaaaa aacagtgaag aaaatctcgg ataaatacca catcaagtta
      541 tcagaagatg aagccggatt tatcgcattg catttagtca atgcacaagg cattcaaagc
      601 cagcaagaca atacctacct gtttactata acaatacagg atattctaaa tatcgtccgc
      661 tattactata agattacatt tgatgaagat tcagtccatt tttttcgatt cgtcacacac
      721 ttacgttttt ttctgacacg cgtcctagat ggagaaaaat cagaagtggg agaagtttct
      781 gaggaattgt ttcaggttat tcaaaaaaaa tataaggata cgtttggttg cgttgagaaa
      841 atagcagact ttttagccga gacgttcaac taccagatgt ccacggacga aaaaatgtat
      901 ttaacgatcc atactgcgcg tttagtgcat gagaatcgtt gtaaataaaa aaaggaggat
      961 aaattaacaa gaacgtatga aataagggta gtgacattca gttggctaaa ccttccacta
     1021 ataaattgga gggaatagaa atgggaaaat accaagaatt agcgagaaaa atcgtagaaa
     1081 atgtcggcgg gaaggaaaat atcaatggtt tgacacattg tatcacaagg cttcgtttca
     1141 aattaaaaaa cgaagaaaaa gcaaatgatg agatcttgaa aaatatggac ggtattgtaa
     1201 cggtaatgcg agcgggaggg cagtatcaag ttgtcattgg gaatcatgtg ccagtcgtgt
     1261 ttgaagaggt tataaaagca ggaaacctta cttttgatga ggctgtttca acagaaaaaa
     1321 tgagaccgtt tgatatgctt attgatggaa tatcgggctg tttccagcca ttccttgcta
     1381 tcctagctgc tggcgggatg attcgtggac tgacagcatt ccttgtcttc ttaggcgcgt
     1441 ttgaccgcgg ttctggcaca tttgttatgt ttgataatat cggtgattct gtattccaat
     1501 ttatgcctgt tattatcggt ttaacagctg cccgtaaatt taaagtaaat gaattcgtag
     1561 ggatgttgct tggcgcagca ttaatgaatc ctagcttgtc gctggaagct ttgagtggtg
     1621 cagcagaagc accactaacg accatatttt ctggcactat ttttgaagca cctatttatc
     1681 aaacagtatt cggcattccc tggattgcgc gtaactacgc ttcttctgtc attcccatta
     1741 tctttattgt gttgttagct agccaaattc agaaaccaat taaaaagtta gtacctgaga
     1801 tgatcgcgaa tttcttcgtt ccattcttca ccgttcttat tacaatgcct ttaggttttt
     1861 tactcgttgg accagtattc acattcgcaa ctgatatttt aatggctgga tttgaaacac
     1921 ttttagcact ttcgccagtt atttatggag caatcgtagg cttcttctgg caaattctgg
     1981 tgatgtttgg tctccattgg gcaatcgtac caatgggatt gatgcaattt tctgtgaatg
     2041 gttggcaaaa tattatgaca cctgttgcag tagtgtcttt tggacaaact gcagcactta
     2101 ccgcactcta ctttaagctt agaaatccaa aagacaaagc aattgcaata ccagccattg
     2161 tttctggtat tgtggggatt acagagccag ctatttatgg gtttacatta ccacgtaaaa
     2221 aaatatttat ttttacctgt gtcggtggtg cgttaggggg cgcgtatagt gggttaatga
     2281 atttaacttc ttggaatcaa ggtggactag gcatctttac aattcccaac tatattagac
     2341 cagatggtga tttaacagat gtgattaatg tattgattgg gatcgccatt gcgatggtgg
     2401 tatcctttac gctgactttt ttcttttgga aggatgaagc aggtgaaaca gatgatatac
     2461 agaaaaaatc aggtaaagaa attgtaaaaa caccgataca aggacaaatc gctccactaa
     2521 atgcagcaaa ggatgcagct tttgcacaag gaacactagg tcgaggaatc ttgatctacc
     2581 ctgaaaaagg agaagtacgt gccccttttg acggaacagt catgaccctg ttcccgacga
     2641 aacaggcaat tggaatggtt tctgaaacag ggctggaatt gctgattcat gtcggattgg
     2701 atactgtgca gttggaaggg aaatattttg aaagcttagt gcagcaagga taaagagtga
     2761 aacaaggcga tctgttgcta cgcttcgaca aaaagaaaat cgaaaaggaa ggatacagtt
     2821 tagaaacacc tgtgatcgtc accaattaca cggattattt ggatattttg gaaaaccaag
     2881 gggagacggt cgggccagac gatgcactta tcaccgcact gacctgaacg gttaggtgaa
     2941 gaatggacta ggcttaacta ttctttttat tccacttctt tattttcaaa attgaaaggc
     3001 tctttcatga tggctgagct gatagggcgt ttatcgaatt tttgaccgac agaaattaga
     3061 tggactaaat tctgttgata attttctgca aaaacacaac tatataacac gtctagaaga
     3121 taacaaattg atgtattgat cgtaaaatta ccaatttttg aatacagtcg ctcgcgtgtc
     3181 gtaagtcgca aagcggctgt actcattgca gatagcgttg aatcaccgat actggtcaaa
     3241 gctaaaattg gaacatgatt ttgtttcaat atttctgcgg tacgaaggat cacattgttt
     3301 tcacccgtat aagaaatcag aatcccgcac atatcactcg tgcaattgta cgcttcatag
     3361 aatgattcac caaaagtggt gacaatcgtg acatccattt tgatgcgctt cattttcagc
     3421 atgaaatctt gagaaatcaa agtattcgag ttactggcga aaatcacgat tttttttgct
     3481 tgaagcatta gttgtttggc tttttgcagt tggtcatgat gaatgagaga aagtgtatca
     3541 tgaatcgtca tctgttctaa agaagccatt ttatttgcga tcgtcataat accgtctgtg
     3601 gtggaaaaag gaagattcgc atctacgtct tgaaaattcc cttcaagata ggctgtttca
     3661 gctaaaaaag cattacgaaa actgacccag ccatcaaaac ccattttttt tgctacacgg
     3721 atgagtgtag aggggtggac ataacatcgc tcagcaatcg cttggatggt catttcgcca
     3781 agtgctggag aatcgcttaa gagaaactgg atcacttcat tttctgaagg agaaaaggag
     3841 atttctttga ttttttcacg taataacatg tagatacctt cttcttattt ttttacgtaa
     3901 attataactt ttttgaaaat gatttgtaaa cggatttata ctatataact taaaatagac
     3961 attttttgac aagatattta caaaaatcca ctagaaaaca gtaaattttg taaatgttac
     4021 tggaaagaaa cagatttcta ttgtttgttt tgtttacaaa aaagataatg aaagtgtaaa
     4081 aaagtaaagg agtgagaaca tggcaaaagg aatcaaaatc gctacgattg gtggaggcag
     4141 cagttacacc cccgagttga tggaaggatt tatcaaaaga tatgaggaat taccgatacg
     4201 agaaatctgg ctcgtagatg tagaagaagg aaaagaaaag cttgagatcg ttagtcagat
     4261 gtctcagcgt atgtgggatg cttctccgta tgaagtcaag atctatgcaa ctctggatcg
     4321 ggaagaagca ttgaaagatg cggattttgt tacgactcag tttcgtgtcg gtcaattaga
     4381 tgcacgaatc aaagacgagc ggatccctag ttattatggg atgattgggc aagaaacaaa
     4441 tggtgctggc gggatattca aagcttttcg taccgttcca gtcgttttgg caatcgttga
     4501 ggacatgaag cgtttatgtc caaatgcttg gctgatcaat ttcgcgaacc caagtgggat
     4561 ggtgacagaa gcagttatca gatatggcaa atgggataaa gtgattggat tatgcaatgt
     4621 gccagtgatg gcgatgatga cagaaccagc aatgcttggc aagaaaaaag aagagctgat
     4681 ctacaagttt gccggtctaa atcatttcca ttggcacaaa gtagcagata ataaaggaaa
     4741 tgaccgcacg gcagaattga ttgatcgttt atacgatgaa gagaatggat taccgaaaaa
     4801 tatctttgat atcccgtttt tcaaagaaca attaaaacaa atgaacatga tcccttgtgg
     4861 ctaccaccgc tattactatc gtgaagaaga aatgctggaa catgctttag aagaatatca
     4921 aacgattggt acaagagcgc agcaagtgaa agaaacagaa gcagagctat ttgaattata
     4981 caaagatcca gaattagacc ataaacctga acaattgcaa aaacgaggtg gcgcttatta
     5041 ttcagacgct gcctgtgaaa cgattgcttc aatctatgga aataaagaaa cacagatcgt
     5101 ggtatctaca aagaatgatg gagctgttcc tgacctgccg gcagattgtg tagtagaagt
     5161 cactgcctat gttggcgcac aaggagcgag aaatgtagcg tttggttctc ttcctgctgc
     5221 tgaaagaggc tggttgcaag taatgaagaa tatggaattg ctgacgatcg aagcagctgt
     5281 gactggtgat tatgggacag ctttacaggc gtttacgatc aatccactta tacgatcagg
     5341 aaagacagca aaacggatca tggatgagtt gtttattgca caccagcagt atttaccgca
     5401 attcaaggag acaatcagcc gattaaaaga agaaggaatc gaagttcaag atgaactggc
     5461 aagagaatta tcaggtaata cacatataat ggaggaagta taatgggatt cagaaaagat
     5521 tttttatggg gcggtgctac agcagccaat caatgtgagg gcggttatga tgaaggtgga
     5581 cgcggactag caaacgtaga tgttgtgcca atcggaaaag accgttttcc tgttatcacc
     5641 ggaaagaaaa aaatgtttga atttgatgag gatcattttt atccagcaaa aattgcaatc
     5701 gatatgtatc atcgttataa agaagatatt gctttgtttg cagaaatggg gttcaagact
     5761 tatcgaatgt ccatcgcctg gagtcgtatt tttccaatgg gggatgagaa agagccgaat
     5821 gaagctggct tgaaatttta tgaggatgcc tttaaagaat gccaaaagca cggaattgag
     5881 ccacttgtga cgattacgca ttttgattgt ccaatgcatt tgatcaaaga atatggtgcg
     5941 tggcgttcca gagaactcgt tggtttttat gaaaatctgt gtcgagtcat cttcaatcgc
     6001 tataaaggac ttgtcaaata ttggctgaca ttcaatgaga tcaatatgat ccttcatgca
     6061 ccattcatgg gcgcagggct gtattttgaa aaaggagaaa atgaagaaca aatcaaatat
     6121 caagcggcac atcatgagtt ggttgcaagt gccatcgcaa cgaagattgc tcatgaagta
     6181 gatccggaga acaaagtagg ctgtatgctg gcagcaggag catattatcc atacacttgc
     6241 aatcctgaag atgtttgggc ttctagaaaa tcagatcgag aaaattattt cttcattgat
     6301 gtccagtcac gaggagaata tcccgcttat gcattgaaag aaatggagcg aaatggcatt
     6361 cagatcgaaa tggaaccagg ggatgccgca ttgctgaaag aacatactgt tgattttatc
     6421 tctttttctt attatgcttc ccgagtttcg actaccgaca acgaactctt ggaacagact
     6481 gcaggaaata tctttgcttc tgtcaaaaat ccatatctgc aagcaagtga atgggggtgg
     6541 caaattgatc cattaggctt gagaataacg atgaatgata tttatgatcg ttatcaaaaa
     6601 ccattgttta ttgtagaaaa cggcttaggt gcagtcgata cgccagatga aaatggttat
     6661 gtggaagatg attaccgaat cgattacctg gcagcgcata tccaggcgat gaaggatgca
     6721 gtagaactag acggagtcga tctattaggc tatacgactt ggggatgtat tgatttagta
     6781 tcagctggta ccggtgagat gaagaaaagg tacggattca tttatgtcga tcgtgataac
     6841 caaggaaatg gcacattagc ccgttcaaag aaaaaatcat tcaactggta taaaaaagta
     6901 attgattcaa atggagaaaa tctttcgaac gattgatcaa tgatttataa aagtctattg
     6961 tttttcagcg gatttttcga gtataataaa gctattcgag aaacgcaagc agaacaatag
     7021 actttttttc gcattggctg taaagtatgc ttacttgatt aagtaaagga gaatctacat
     7081 atgtatgatg taatcgttat tggcgcaggt cctgctggga tgactgctgc tttatatgct
     7141 tcacgttcaa acttatccgt attgatgatc gaacaaggtg cccctggcgg ccaaatgaac
     7201 aatacggcag aaatcgaaaa ttatcctgga ttcgattcga tcatgggccc cgatctagct
     7261 tataagatgt atgaaagtgt ctcaagattt gggacagaaa atgcttatgg aatcgttcaa
     7321 gatatcaagg atcacggcag ccataaagaa gtcatctgtg aagataaaag ttacgaagga
     7381 aaaacagtca ttatcgcaac aggatgtatc caccgtaaat taggcgtacc tggcgaggaa
     7441 gaatttgctg gacgaggcgt ttcttactgt gcagtctgtg atggtgcgtt tttccgtaac
     7501 aaacgtctag tagtcatcgg gggtggggac tcagcagtag aagaagccat ctatttgaca
     7561 caatttgctt ctgaagtagt gatcgtccac cgtagagatg aattacgtgc acaaaagatc
     7621 atccaagacc gtgcatttgc aaatgaaaaa atttccttta tgtgggatac agtcgttgac
     7681 gaaatcgttg gaaacgaaat ggtagtgaca ggtgtgaaag cacgtaatgt caaaaccggc
     7741 aatgaatttg atttagcagc agacggagta ttcatctatg ttggactgga tccattaact
     7801 gcacctttta gagaagcagg tttgacgaac gaagctggct ggattccaac agacaacgaa
     7861 atgaaaacga atatgccagg cgtttttgcc atcggagatg ttcgtgaaaa agatttgcga
     7921 caaatcacta cagctgttgg tgacggcggt attgctggac aacaagtgta taaatacttg
     7981 gagtctttag aagaaacaac agcataagtt taaaaagagt agaagaacgg gttcaattgt
     8041 tcttttactc ttttttttcg cgtgaaattt actgaatctg atttatgcga acacgtccaa
     8101 caccttttga taaaagaatc ctttcttgat ttaagcgttt tttggtcctc atactttcat
     8161 atctaataag tgaatgctat acttattaag aaccatattt cagaaagagg tgttattttc
     8221 atgtcttggg aacaagtgta tgaacaatgg gtacatgaag aaaatttaga agaaaactta
     8281 aaaaaacaat taaatgatct gggtgaagat cctgaaaaac ttgaagatgc tttttatgca
     8341 ccgttggaat tcggtactgc aggtatgcgg ggaatcttag gacctgggat caatcgaatg
     8401 aacatctata cggtaagaca agcaacagaa ggactagcac gtttcatgga tacacaaggt
     8461 gaagaaacaa aacgccgagg tgtcgctatc gcttatgact cacgccatca gtcaccagaa
     8521 tttgcaatgg aagcagccaa aacactagca catcacaata tcccgtctta tgtattcgaa
     8581 agtctgcgtc caacaccaga gctttctttt gcagttcgtc atttgaagac gtttgcggga
     8641 atcatggtaa ccgcatctca caacccagcg gcttacaatg gatataaagt ctacggagaa
     8701 gatggcggac aaatgcctcc tgcagacgca gatgcattga cgaaatatgt ccgtgaagtc
     8761 agcaatccgc taaaagtcga agtattatct gacgaagaag ctaaacatag cgggttgatc
     8821 acgatcatcg gagaagaagt ggacgctgct tatctagaag aaatcaaagc agtgacgatt
     8881 gatcgtgaat tggttgaaac aatggggaaa gatttgaaac ttgtgtacac gccacttcat
     8941 ggaacaggga aaatgttggg cgaacgtgcg ttgaaacaag ctggattcga acaattcgta
     9001 ctagttcccg aacaagctgt ggcagatcca gatttcacta cggtcaaatc gccaaatcca
     9061 gaagaacatt cagcttttga atatgctata cgacttggag aaaaagaagg agctgacttg
     9121 ctgattgcaa cagatcctga tgcagaccgt ttaggcgcag cggttcgttt gccagatggt
     9181 tcttatgaag tattgactgg gaaccaaatc ggagcattgt ttgctcaata tattttggaa
     9241 gcacatcaat caagaggcac tttaccagaa aacgcagcgg tattgaaatc aatcgtttcc
     9301 agcgaattgc ctacagcaat tgcggaacac tacggtgcta ccatgttcaa tgtgttgaca
     9361 gggtttaaat tcattgctga aaaaatccaa caatttgaag aagaccattc acatactttt
     9421 atgtttggat tcgaagaaag ttatggttat ttagttaaac cttttgttcg cgataaagac
     9481 gcgatccaag cattgctttt attagcagaa gtagcagctt actataaaaa acaaggcaaa
     9541 acgttgtatg acggcttgca aacgattttt gaaacctatg gctatttcgc agaaaaaacg
     9601 atttctgtga caatgagcgg acaagaaggt tctgcgaaaa tctctgcttt gatggaaaaa
     9661 ttccgtaaag aagcaccgac agaatttgcc ggagcaaaag ttattcaaac agaagacttt
     9721 aaagaattga cgagaacaga ccgtgaagga aacgtagaaa aactaacaac accgccatcc
     9781 gacgtattga agtatacatt ggaagaaaat ggctggattg ctatccgccc ttcaggaact
     9841 gagccgaaaa tcaagtttta tattggtgta aaaggaagtt ctacagaaga cgcagccgaa
     9901 aaagtagcct cttatgaagc gattatcaaa aagatgacag aagaataaaa aacttgagac
     9961 tgatggaaaa agtggtaaac tttagaagtc agtctttttt caagttggag gtgaatggat
    10021 tgccagacac gcaaaaatca aaagtagaga aagtcagtca attattcaaa atgttgagtg
    10081 atcccactcg tcttaaaatt ctgctatatc tgaaagacgg ggaacaaaat gtgacagcaa
    10141 tcacccaagc agtggaaatg gagcagtctg ctgtatcgca tcaactacgt ttattaagag
    10201 aaaatcatgt cgtcaagtct catcgcgagg gaaaagcgat tttgtatagt cttgatgatt
    10261 ctcatgtact ggatattttg aaccagacac tcaaacatgt ggaacatcaa taacgatagg
    10321 tgcaaataga taaacactgg agtgtgacaa cgaagaaatt tcgttgtcac actccagtgt
    10381 tttttaagaa aaagacaaaa atgatcaatc gcttttttct gttgtttcag ttccataata
    10441 actatgatct tctaggtcta aagcattcaa aataagcgaa gccgtttctt caatagataa
    10501 agatgctaca ttgatcacta cacatcctag tttttcataa agatcattcg caaaatcgag
    10561 ttcagctcta attttttcga tatcggaata agcagtatca gggtttaatc cgtaagagcg
    10621 catccgttct ttacggatat tatttaaaat atctgggtta tttgttagtc ccacgatttt
    10681 tttaggatcg atctcccaaa gctgtttggg aatatgtgct tgtgggatca aaggtaaatt
    10741 ggcaactttt aaatttttgt tagctaaaaa caagctcaaa ggcgttttgg aagttcggga
    10801 tacgcctagc aaaacgacat ctgcttctaa aaaaccacga ggatctttcc catcatcgta
    10861 ttttacagca aattccattg ctttgatccg tttgaaatag ttctcattca ataaatggag
    10921 ggcaccaggt tgccgaattg gcttgatacc agttaaacgt tcaatttctg atatcggtgg
    10981 cgtcatcaca tcaaaactaa ataattgatg ttcttcacaa aaagaacgtg ttatctcgat
    11041 cagttcttga ttgattaatg tatgcaggac aagtgctttt tcttctaatg catcttgcaa
    11101 tgcctgcctt aatgtttttt catctgtcac gaacgttctg cggaataggt tgaactcaac
    11161 ggtaggatac tgagccatag tagcttgcgc tagttttgtc gcagtttctc cagcagagtc
    11221 ggagatgaca aaaaaagtaa cgatttctgt agaatcggaa gtcatggata aaacctcact
    11281 tgcatttttt ttatgtttat tataccatac tctagaatgc gtacttcgta atcattttga
    11341 tttggaggga taagtcatga aaaaacagac attgatcgaa caatcattag aaacattgaa
    11401 aaataacggt tttaaatata ctaaaaaaag agaagcattg ttaacttatt tagtgaagcg
    11461 taaccgctac gtatctgcta aagaagtttt tgactatatg aatcaggaat tcaaaggggt
    11521 aagttatgat acgatttacc ggaatctcca tgattttgca gagatcgatt tactggaaga
    11581 aaccgattta aatggagaaa tgaagtttcg ttttcattgc tgtcagggga aaatcggcca
    11641 tcaccatcat tttatctgta cagtgtgcgg aacaacgaaa gaaatccata tgtgtccgat
    11701 gaattatttc gaagaacagc taggtggatg cacgatcgaa gggcatagat tcgagatttt
    11761 cggaagatgt gaaaattgtc aataaggatt taaataaaat aacatttgat aattttaaaa
    11821 aaaagcaaga tttccttgac ggtcaaaaca gcattagata taatatttac aggacagtct
    11881 tgttttttga gatatatttc aataacaatt acggttactt tttaagctcg gagggaggga
    11941 attaacatgt caaaaacagt cgttcgcaaa aacgaatcac ttgacgatgc tcttcgtcgc
    12001 ttcaaacgtt ccgtgtcaaa agcgggtact ttacaagaat ctcgtaaacg tgaattctac
    12061 gaaaaaccaa gtgtaagacg caagaaaaaa tctgaagcag ctagaaaacg caaaaaattc
    12121 tagttctgtg atgaattgga gttggtagtg tgtcactact aagtagatta aacgatgaca
    12181 tgaaagcagc gatgaaagcg aaagataagg aatctttaca agtcatccga atgatcaagt
    12241 catctataca aaacgagcag attaaagaag gtcacgactt gacagaagaa gaagaactaa
    12301 cggtgttgtc tcgtgaaatg aaacagcgac gtgattcttt gcatgagttt gaagaagcag
    12361 gacgtgacga tttagctgaa aaagtaaaaa gtgaaattgt aatagtcgaa aaatatatgc
    12421 cggaacaact ttccgacgaa gaaattcgac aacttgttca agaagcaatc actcaaactg
    12481 gtgcttcatc aatgaaagaa tttggcaaag ttatgggtgc aattatgcct aaagtcaaag
    12541 gaaaagcaga tggaaaccaa gtgaatgcga ttgtgaaaga actattacaa tcttaatttt
    12601 atcatttaaa ctgcggacag tgattatccg cagttttttg tgttcgtaaa aaaagaacta
    12661 aaaaacagct agaagaaaga atcttattct t
//
LOCUS       DAZUMK010000078        12578 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000078 DAZUMK010000000
VERSION     DAZUMK010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.080"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..2376
                     /locus_tag="ACG808_001969"
     CDS             4..2376
                     /locus_tag="ACG808_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFS5047586.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNDQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERIENKVQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     TPTDSVKEKETDHFYRVEFNENDPAKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELARLCEQVPEKEQKQTVKNEPISKV
                     QNEIKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DQTGHTELQVGIQAVAKNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     ATWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQD
                     EKNVVETAEQKNIVLVGSLWIDKKDGKVLEEDTGRTLHLKDAAFIEDTQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2437..3897
                     /locus_tag="ACG808_001970"
     CDS             2437..3897
                     /locus_tag="ACG808_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFS5047587.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPDWLIDYAEKEGLIAELKPKHERQNF
                     LVQDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEMKVEPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKGCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIHQQEIRKQKRAMLFQQGKQQERE"
     gene            3908..6112
                     /gene="topB"
                     /locus_tag="ACG808_001971"
                     /pseudo
     CDS             3908..6112
                     /gene="topB"
                     /locus_tag="ACG808_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
     gene            6128..6277
                     /locus_tag="ACG808_001972"
     CDS             6128..6277
                     /locus_tag="ACG808_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047588.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6358..6954
                     /locus_tag="ACG808_001973"
     CDS             6358..6954
                     /locus_tag="ACG808_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFS5047589.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDTERTLCDIWNPWYTVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            6967..7866
                     /locus_tag="ACG808_001974"
     CDS             6967..7866
                     /locus_tag="ACG808_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFS5047590.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNEIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKEQKLAYQRHQQMNREMER"
     gene            7869..8353
                     /gene="ssb"
                     /locus_tag="ACG808_001975"
                     /pseudo
     CDS             7869..8353
                     /gene="ssb"
                     /locus_tag="ACG808_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            8375..8632
                     /locus_tag="ACG808_001976"
     CDS             8375..8632
                     /locus_tag="ACG808_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047591.1"
                     /translation="MAKKISLTMDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVF
                     VYLVCSYLLKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(8791..8925)
                     /locus_tag="ACG808_001977"
     CDS             complement(8791..8925)
                     /locus_tag="ACG808_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HFS5047592.1"
                     /translation="MLKSIKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK
                     "
     gene            9068..9328
                     /locus_tag="ACG808_001978"
     CDS             9068..9328
                     /locus_tag="ACG808_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047593.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            9777..9983
                     /locus_tag="ACG808_001979"
     CDS             9777..9983
                     /locus_tag="ACG808_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFS5047594.1"
                     /translation="MRKKFDYLGVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIEEKGKKR"
     gene            9983..10234
                     /locus_tag="ACG808_001980"
     CDS             9983..10234
                     /locus_tag="ACG808_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047595.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGCELHYQENLETYENEYWLTKLEN"
     gene            10250..10687
                     /locus_tag="ACG808_001981"
     CDS             10250..10687
                     /locus_tag="ACG808_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047596.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCFEIGVKPSTIKPSEYQALE
                     LTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            complement(10798..11025)
                     /locus_tag="ACG808_001982"
     CDS             complement(10798..11025)
                     /locus_tag="ACG808_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047597.1"
                     /translation="MKQNTSITKNTARFNCTAALSRISISPTNSTNNKGIIFFKWITF
                     FLLSYRALPVYFAPPKRRGRLVRNKNHSLSY"
     gene            11123..11311
                     /locus_tag="ACG808_001983"
     CDS             11123..11311
                     /locus_tag="ACG808_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047598.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IACIGQYLYCIRGRKLYA"
     gene            11429..11635
                     /locus_tag="ACG808_001984"
     CDS             11429..11635
                     /locus_tag="ACG808_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047599.1"
                     /translation="MKYNLLDHKRMWVEKLEEDLEQLELLGHNKTSTVYKEGLKRTKK
                     AQKELATLESRVQKKEVDLTHTYL"
     gene            11645..11878
                     /locus_tag="ACG808_001985"
     CDS             11645..11878
                     /locus_tag="ACG808_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HFS5047600.1"
                     /translation="MADKRKEKIQKLLALAADVNDEESVSALAKAQQLMMDYDLTEDE
                     IFNYHEQQRIEAVETHVIYRGRPQKWLYRLFRN"
     gene            12200..12403
                     /locus_tag="ACG808_001986"
     CDS             12200..12403
                     /locus_tag="ACG808_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucocin A/sakacin P family class II
                     bacteriocin"
                     /protein_id="HFS5047601.1"
                     /translation="MILGIVLLSVSTLGITVDAATYYGNGVYCNTQKCWVDWSRARSE
                     IVDRGVKAYVNGFTKVLGGVGGR"
     gene            12393..>12578
                     /locus_tag="ACG808_001987"
     CDS             12393..>12578
                     /locus_tag="ACG808_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047602.1"
                     /translation="MEDKKEKLFDLIANAYNSKSYNNVEVKEGFLQDAKKIREDKPYP
                     EIIISVYKHLSKLKAEER"
BASE COUNT         4655 a         2077 c         2645 g         3201 t
ORIGIN      
        1 gacatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ttagtacgat tgcaaaatga attaaatgat caaatcgaaa agatgtttga acaccaaaga
      121 aagaccaacg gtcaaccaat gaacgataag agaaacgggc attcttggtt taggcaacaa
      181 gaacgaatcg aaaataaagt ccaaagtctg agagaagaga tcaagcaaca agaaaaacag
      241 gtagaaaaac ttgaaagaca agaagagcta aaggaaatgg gttataacaa gtacggtggc
      301 ctggatatga cgatcgaaaa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcga ctttttcagc tgccacgatc cgaaaatacc aacgaaaatt ggaaacatta
      421 gaacagctga aagaaaaatc agagaaagga aaagaaaata ttcttcctga agtacaagcg
      481 atcattgaca gcggacgagt gacgcaatgg aaaaagaatc caactatcta ttttctgaaa
      541 ggctatcgaa aagtcgcttt agaactcaat gaaaacgggg gatttgagga atctaaaaaa
      601 taccaagcca cgtcggaaga agaaaaggca gctgttcgtg aattacttga agaagctaca
      661 cctactgatt ctgtgaagga gaaagaaacc gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag caaaagggtt gaaaagttat ggtggggaga tcgtaacact agaattgatc
      781 gatcaactca aaacactgga taaccaatac gccgcagaag caggctatta taaattctat
      841 tttgaagaag tgaagaatgg agaagtagta gatcagatac gaatggatct aggggatggc
      901 gtcaatagta atcaccccat ctatttagag ttagctagat tgtgcgaaca agtacctgag
      961 aaggaacaaa aacaaacagt caaaaatgaa cctatttcaa aagtacaaaa tgagattaaa
     1021 gagacaaaaa caaaacaaaa gggttatcaa aaacgaacgg cagaagagat taagcaagaa
     1081 gtcaaatcac tttcccaaca ggcgcttgat gcggtgaaaa agcatactca ttcacctgaa
     1141 gacgtcaaag aacttttgaa ttttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgatcg aggaacaatt tccaggcgca acggcttgtt taggtcgtgc cgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaaggat tttatgagaa aggacgtggc tttgttcgag aaacagaggc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca aaaaacctta ctacacgatt
     1441 gaaaaagtgt ttgatatcac acaaacacaa ttgaagcctg aagactatcc gaaaatcttc
     1501 ccgaaccgcg tctttgattt tcaattggat caaacagggc atacagaact tcaagttggg
     1561 attcaagcgg tagccaaaaa tattggaatt caaattcgag atatgacaga aagtgaagtc
     1621 tatcaaagag aattaggaca agcacgtggg gcgtacgttc gtaacgaatt tactggcgaa
     1681 gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttagccac aagtattcac
     1741 gaattagccc atgctagaat gcacaaattt tcagaattag atactgcaac aaaagaacta
     1801 caggcagaaa tgacctccta tattgtgtgc aagcattttg ggatggacac ctcggagaaa
     1861 gctattcctt atattgcgac atggacgaag aatggtcaga cgttggatga taaggaagct
     1921 gcggaacgtg ggaaaatcat gaatgatgtc agtcgtgtcg caaatgaatt cattcaaacc
     1981 atttctaatg aaatcaatca atacagagaa agagaaccag agatccagcc agcagaagtg
     2041 aagcaggatg agaaaaatgt tgtagaaaca gcagaacaaa agaatattgt gctcgttggc
     2101 tcactttgga tcgacaagaa agacgggaaa gtcctggaag aagacacggg ccgaacgtta
     2161 catttgaagg atgcggcgtt tattgaagat acgcagcata atcaagtgat tttaaaagga
     2221 aaaagttatg gaaaagagat tcaaacggcg ttaccttcag aagaatttgc gctaggaaat
     2281 ccattccgat tttcacttga aacagtgtca gaagtggcca aacgaaaaaa tgaaatctat
     2341 tatgaacaac aattgtcaca aaaagaacgc gtataaaaca agtaaaaaag actatctgaa
     2401 aaacggatag tcttttttct tttgggaggt ttgaaaatga ccgagaaaaa aatgagtctt
     2461 attgatcgat gtaaacagat cgatatagtg gattttgctc gaaacaatgg aatggctgta
     2521 gtcaataaag gacgagacta tcgcttagaa gaccatgatt catttgtttt tgatcgaaga
     2581 aaacaacgtt tttattggaa cagtcaaaat attagtggcg acattattga attagctaaa
     2641 ctttttttca tagacaaaga gattcaagac tctaagcaac aatttaaagc agccacagat
     2701 tttattctaa agaatgaaga caaaacagaa cgagtagaaa atcttcattt tgagacagaa
     2761 aagtacaaag atcaccccgt tgattatcaa ccactcacag aaaaaggaag gaattatttg
     2821 aaggaagaac gaaaactgcc cgattggttg attgattatg cagaaaaaga aggattgatc
     2881 gccgaactaa agccaaaaca tgaacggcaa aatttcttag ttcaagacga tcgtttggat
     2941 catgcggtcg cctttttatg gaaagatcca cagacaaaag agacagtagg ggcgagttat
     3001 caaggaacat tcattgatta tgaacgtttt ggtgagagag gcacatataa gcatatagat
     3061 aaaaattcga cagcgaatca tggatttaac ctaaagatcg gtgaccctaa acagttaaaa
     3121 ttttttgaga gtagtattga tttacttagc tatgctgcac tgaatcgtga tcagttaaac
     3181 gatacgtggt tagtctctat ggaagggtta aaacaccatg tgatcagtca ttattttggt
     3241 gaggcagtat ctgaattgag aaagaaacaa gcctttcctc aatccattga gatttgtgtg
     3301 gataatgacc gtgcaggaca tattttctac gaaaaagaac aattaatggg cgcagttgat
     3361 ccatttacga atcagaaagt acgttgtgaa cgaggaatcg ctaatgattg gcaagtgcca
     3421 aaagaataca aagtcattta tgaagaagtt gcaaaagaaa tgaaagttga acctgaagca
     3481 attatggcca tacacaagac agaaaacaat ctgcaattaa cgaaccaact ggtttccgct
     3541 cacaaagtaa atgcgtcttt tggtcagcag ctttctgtta acgattcaat agaagctatt
     3601 aatttgaaag atatttgtag agaagtcgca aaagaattaa aaggttgtga acgcgtggat
     3661 ggtacgtacg attttgatcg tttttatcaa gaaaaagggg atattaatgc tcagattctt
     3721 ttctcttata aagcagaaca atactacaaa ggctataaaa atcatgaaca tgaatttgta
     3781 cctgaagtaa aaaaggattg gaatgatcaa ttgaagcatg aaatccacca acaagagatc
     3841 agaaagcaaa aacgtgcgat gttgtttcaa caaggcaaac aacaagaaag ggagtgaaat
     3901 cgtcatgatg aaaaccgtta ttttagcaga aaagccgtca caagcgaaag cttatgccga
     3961 tagcttctct aaagccaccc gaaaagacgg gtattttgaa atccaagata gattgtttcc
     4021 tggtgaaact gtgatcacgt atggttttgg tcatttagtc gagttagatt cgcccgatat
     4081 gtacgatgaa aattggaaac aatggtcatt agagcatttg cctattttcc ctacacatta
     4141 tcactatcat gttcctaaag ataagaaaaa gcaattcaag gtggtgaaac agcaacttca
     4201 atcggccgac accattatta ttgcgacaga tagcgatcgt gaaggggaac tgatagcttg
     4261 gacgatcatt caacaagcag gcgccgatca tggaaagata tttaaaagac tttggatcaa
     4321 ctccttagaa aaagaagcca tttatcagtg attccagcaa ttacgagacg cagaagagac
     4381 ctatcccaaa tttgaagaag cacaagcacg ccagatcgct gattggttga tcggcatgaa
     4441 cggcagccca ctgtatagtt tactcttgca gcaaaaaggc ataccaggaa gtttttcctt
     4501 aggcagagtc caaaccccaa cgctttacat gatttatcag ctgcaggaaa agattagaaa
     4561 cttccaaaaa gagccttatt ttgaaggaaa agcccaagta atcgctcaaa atggcgcatt
     4621 tgatgcgaag cttgatccga atgaaaccca agcgacgcaa gaagcatttg aagattattt
     4681 aaaagaaaag ggggttcaat tgggaaaaca gccaggtacg atccaccaag ttgaaaccga
     4741 gaaaaaaagt gcggcaagtc cacgtctttt ttctctatcc agccttcagt ctaaaatgaa
     4801 tcagctcatg aaagctagtg ccaaagatac tttggaagcc atacaagggt tgtatgaagg
     4861 gaaatatcta agctacccac gaacagatac cccttatatt acagaagggg agtatgcgta
     4921 tctgttggat cacttagacg aatacaaaca ctttttaaag gccgaagcca ttcctactcc
     4981 tattcataca ccgaatagtc gatatgtgaa taataagaaa gtccaggaac attacgcgat
     5041 cattcctaca aaaacggtca tgacggcagc ggcttttgaa caattaagtc cactgcagca
     5101 ggccatttat gaacaagtgt taaaaacaac ggtggccatg tttgcggaaa aatataccta
     5161 tgaagaaaca acgatcttga cgcaagttca gcagcttcag ttaaaggcaa tcggaaaggt
     5221 gccgctagat ctaggttgga agaagttatt tggaaaagag agcgacggaa aagagaagga
     5281 agaagagccg ctacttccta aagtgacaaa aggagaaatg gtgacggtgg atctacaagt
     5341 tcttgaaaaa gaaaccaagc caccacaacc ttataccgaa ggtacgttga tcactgcaat
     5401 gaaaacagct gggaaaaccg tggatagtga agaagcgcaa tccatattga aagaagtgga
     5461 aggaatcggt acagaagcga cacgggcgaa tattattgaa accttgaaac aaaaggaata
     5521 tatcaaggta gagaaaaata agctcgtggt gacgaataaa gggattttgc tttgccaagc
     5581 cgtggaaaaa gagccgctgc ttacgagcgc cgaaatgacc gccaaatggg aaagctactt
     5641 gttaaagatc ggtgaacgaa aaggaacaca aaccactttt ctagccaata tccaaaaatt
     5701 tgtctctcac ttattggaag tcgtaccagg gcaaatccaa tccactgatt ttggatcgac
     5761 gttacaagaa gtgaaagcag ccagtgaaaa gcaagaagcc gcgcgtcatt taggaatttg
     5821 tccaaaatgc caggaacaag aagtcttgct ctatcaaaag gtggcagctt gtacgtctga
     5881 agcctgtgat tttaaattat ggacaacgat tgccaaaaag aaactcacag cgacacagct
     5941 gaaggagatc attcaaaata gccgaacaag ccaaccagtg aaagggttaa aaggtcagaa
     6001 gggttcattt gaagcaacga ttgttttaaa agaagacttc actacaggct ttgaattttc
     6061 tgaaaagaaa aaaacgaact tcaagaaaag aacacgccgc actgcaaaat aatggagggt
     6121 taatcttatg cgtaatacca cacacaaaca acaagaaatc gaaaatcaaa aagccaaaat
     6181 tcgttcagaa caggaaaaaa tgcgtgccag caaacgaaga ctgcagcaat tgaagaacca
     6241 gcagaggatt caaaaagaaa tcgaattaga tcgataaaat cttcctaact attgtatacg
     6301 tataaactta tgttatactg tttttataag tttatacgta tacaaaggag ggagaaaatg
     6361 acaaaagcaa tggtatacaa gctgctagaa gaatttaatg gaactttggc tttgaaggat
     6421 gccaaaaaag cgggcatttc tcctgtaacg atcaaacgta tggtagatcg aggggagctg
     6481 gatcgagagt atccaggttt tttcacctta cctggtcaat ttcctgatga attgtttatg
     6541 gcgcaaaaaa aatatgaacg cggcattatt tcacatatta ccgcgttaga tttgtatgat
     6601 ttaacagata tgatccctag acagatcgat ctgactgtac cttacggtta tcacgtttct
     6661 gaaaaaggat tgaaggaatt cgcagttgag ctacactata caaaagcgga atggtatgaa
     6721 ttaggtaaga ttgaaataaa aagtcgctat gggaatccta ttatagctta tgatacagaa
     6781 agaactttat gtgacatttg gaatccctgg tataccgttg aggatgaaat caaagtaaaa
     6841 gcaatcaaaa actatatgga gtcggacaga aaaaatctta gaaaattgaa tgagtatcga
     6901 cgaattttac caacagacaa aacgatgcgt tcttatatta tggctttaaa ttaaaggaga
     6961 agatacatga cacctacgca gttaaaagca aaagtgaaga atatttctag agaaaaagaa
     7021 gttgatccgc agcttgtttt aagacatttt atgatggaaa aattcttgga aaaaatctca
     7081 gagtctcctt atcgtgaaaa ttttgtgtta aaaggcggct tcttgattgg ctcaaagtat
     7141 ggcattgaaa atcgcacaac caaagatatt gatacgactt tacgggaaat gaaagtaacc
     7201 aaagaaacat taacaacggt tctaaacgaa attttttcaa cgccgacgaa agaaggcatt
     7261 caatttgaga ttcaaggaat gaaagaaacg agagaagccg attactatcc aggatttagc
     7321 ttacgtgtgc tggcacattt ggaaaacatg cggccagatt ttaaggtaga tgtgactaca
     7381 ggtgactcga tctatcctgc aacgatcact cacagtcata aattgatgtt tgaggatcga
     7441 acgattcctt tggaatccta tcctacagaa caaattattg ctgaaaaatt aagtgcaacc
     7501 tttgattttt taacagataa ttcgagagga aaagattttt atgatctcta tactattcct
     7561 aaaatggaga agatcgatga gaagaccttg aaagatagtg tgagaaatac atttacacgt
     7621 cgaggaaaaa ctgatccgtt aaagatatac tatgaaaagg atatggatgt actgaaaaat
     7681 agtcacgagc taaaagatac ctggaagaaa tatcaaaaaa gcaatcctta tgctgcatcg
     7741 atcacgtacg atgagacaat ggattcgatt agtaatttga tggaaaaagt gattcacgtc
     7801 gaagaaaaag agcaaaaact agcttatcaa agacaccaac agatgaatag agaaatggag
     7861 agataaagat gattaataat gtgacattag taggacgctt aaccaaagat cctgatttac
     7921 gttataccgc aagtggtact gcagtagcga cttttaccct agccgtcaac cgaaatttca
     7981 cgaatcaaaa cggcaatcga gaagccgatt ttattaactg cgtgatttgg cgcaaaccag
     8041 cagaaacaat ggcgacatta gctaaaaaag gtagcttgat tggtgtagtt gggcggattc
     8101 aaacgcggac ttacgacaac cagcaaggac aacgtgtata tgtgactgaa gttgtagcag
     8161 ataactttca actattagaa aataaagcag cgacagagag ccgtgcgcac gctgatcaat
     8221 cgtccaccag tccaagcacc gccacctttg aacaacggat acagcgacgc ctaacaacaa
     8281 tggattgaac gcttcacaga atccatttgg tggacaatca atcgatatta gcgatgatga
     8341 tctaccgttt tagtttgaga aagaaggagt aagcatggca aaaaaaatta gtctgacgat
     8401 ggatctattg atacccgtaa ttgcatttat agacgggatt tctttgggcg tcactgaaaa
     8461 tcctttggta aatagaattg cgaaaggatc actatttctt gtattcgtgt atcttgtctg
     8521 ttcttatctt ttaaaaagag caggaaagga taattccttt tggcgattga ctaaagtcac
     8581 agtagcagcg acagctgtta tacttacggc tattctaccg ctttatatgt agagttaacg
     8641 ggaaatcgtt gaacttcttg cgaaatattg ctataatgaa catgaaaaag aggagttgcg
     8701 ctaacaaccc ctcattaaca gcaccgatta aagaacggtg gcagaatatt agttatttgg
     8761 aaataaccgc tgacctgcta aagtagacgg ttatttcttt ttgtcatttt taagcatatt
     8821 tacaatcaaa ctaatcaaag caatggtaaa cataccaaaa ccaagaatgg tttgaattgt
     8881 ctcatatgcg gacaaaaggc ttactccttt tatagatttt agcacttaca ttcataagca
     8941 ccaccacctt tccagaagtc gccaccgtct tcaaacttta ctgtcttgac tattatagca
     9001 tatctaaaat cgaataaaaa ttctcaattt taactgtttt tgctataatt ttagcgaggt
     9061 gatcgtgatg aacaaatatg ttggtttgct tgataaaatc cgagtaataa aaacacaacc
     9121 attattggtt cgctttactt tacaaacaat tcatgaatcg attaattgtg tagttgcaga
     9181 tattgaaatt atcgataaat tgttaattat ggatgatgga aaatacaata ttgccgtcac
     9241 aggtcatttc aataaacgga atcaattagt cattgaatcg atgcaaatta gaaaccctga
     9301 tcattttacg cgatcaatgg gcatataaat aaaagaagag ctgcaatcct aatgtaggac
     9361 tgcagctctt tttgttttac atagaaacag gaagaaaggg aaaggcctgt ttaggaaggc
     9421 acaagtgcct tctcaattga aaaacctttc tctcactcca accgccaccg ccgttcgaga
     9481 agaaaggaag tgaacgttgc acgttcacta agataagggg tcttgctttg taagacaaaa
     9541 taggaaaagc ttcctctcac caaaaggttc gttcctgttc gctatctctt tattgatttg
     9601 ccagaccggc aacaagttgc cttttatcac caaaaagcga caaggacaag ggtaaatggc
     9661 acgctgcgcg cccttgtcct tgtcgctttt cgttgattca ttctggccaa caaatcaata
     9721 aagaaagaac taacgaaagg aagtaaaacc tttcctaaga ggaggcagaa tcaatcatgc
     9781 gaaagaaatt tgattatttg ggtgttccct tcagcgaatt attcccaaac tatcatgcac
     9841 cacatactgt cgaatgcgat tgtggagaaa gagcgaaatg tatcaaaagc tattgtttgt
     9901 atcaatgtcc aacatgcggg aaaaaatata ccttgtctta tggcgattat attttgattg
     9961 aggagaaagg aaagaaacgc tgatgaaaac acaacacaaa ttattgactg taaaagaagt
    10021 cgttcaaatt ttggtggaca aagaagtcat ttctgaaaaa gtagcagaag ctttacttat
    10081 tccgcggtac tggacgatag aaaccccttt agtagaggat gcaccgacta tgctatttga
    10141 agacctagtg aagattgcag gatgtgaact gcattatcaa gaaaacttgg aaacttatga
    10201 aaacgaatat tggttaacca aattagagaa ctaaaaagga gaaaaaaata tggctcaaaa
    10261 ttactatgac gaatttgtca aattaccttt ggataaaatg gcacaaaaaa tggaggatat
    10321 gacgttccta tacaacgaaa cacgtgttcc aaaaaaacat tataaagaaa aattgtcggt
    10381 agctgtggaa gaaatgattg aatcaggtgt cgaaatgaat cttattgcga cgtactatcg
    10441 aacactggaa gaactgaaaa agcaaaatgc aaaatggttt ttccaagcct tactatgctt
    10501 tgaaataggt gtcaaaccaa gcactattaa accttctgaa taccaggctt tagaactaac
    10561 gtatgcgaaa tttatcgaaa cgaaaaaagc caagactgtt tcaagtgaat ggttagatta
    10621 ttttgaaaat atcaataagt atggcgcata ttacaccatg aaaaaggaag acaacgaaaa
    10681 tgaataaggt gatttatcaa ctaacaacgg attccatatc aacgatttga aagaaaaaaa
    10741 ggtcaataat catttatacc gaaaagtgag aatgtgtgga ataaggacaa aaaaagccta
    10801 gtaactaagg gaatggttct tatttcgcac caaccgcccc cgccgcttag gtggtgcgaa
    10861 ataaacgggc aaagcccgat aacttaagag aaagaaggtg atccatttga aaaagattat
    10921 tcccttattg ttagtactgt ttgttggcga aatacttatg cgagatagtg cagctgtaca
    10981 gttaaacctc gctgtatttt tggtaatact ggtgttttgt ttgatctttt ggcggtttgt
    11041 attagtcctc gtgagtctac tattcaagat gattgcagcc atatttttca tggggttatt
    11101 tcgatgaaag gagagagtac caatgccaat tgcaatagga aataaacggt tacccgtgac
    11161 attagatgaa aagagacaaa aagaattgca gcaactaaag cagaagtacg gcaaaagtga
    11221 atccaggatt atgtgtattg cgttagattt attgattgcc tgtattggac aatatctcta
    11281 ttgtattcga ggaagaaagc tttacgcttg aatgcgataa gttaacccga tatatcgatc
    11341 caaaattcta tgaggataaa aacctggcat gggaactggc tttatagaca gagtgctagt
    11401 gcaagtaaag gaagaaagga agatcaccat gaaatacaat ttattagacc ataagcgaat
    11461 gtgggtagag aaattagagg aagacttaga acagttggaa ttgttaggtc ataataagac
    11521 ttcaacggtg tataaagaag gactaaaacg aacgaaaaaa gcacaaaaag aactagcaac
    11581 actggaaagt agggttcaga aaaaagaagt agacttaaca cacacttacc tttaggagta
    11641 gaaaatggca gacaagagaa aagaaaagat tcaaaaatta ctggcgcttg cggcggatgt
    11701 aaatgatgag gaatcagtga gcgcattagc caaagcccag caattgatga tggactatga
    11761 tcttactgaa gatgagattt tcaattatca tgagcagcaa cggatagaag cagtagaaac
    11821 gcacgtgatc tatcgtggac gtccgcaaaa atggctgtat cgtttattta gaaattaaga
    11881 taaaaaaggc tagttaacag gtttgttgac tggccttttt tgatatgttt gtacatgttt
    11941 tattgtgttt taatttttct cttttttata gcaagctcct tttataataa atattccgaa
    12001 aagtgaagac cattttttat ttattaagac tattattatt agtgagataa aatttgattg
    12061 atcttacaga atattggtga cgattatgac actaaagata ctatttatga aaattttgca
    12121 aaaatgttct gaaaattaca tataattgtt ttataaaaaa gggaggcaat tatatgaaga
    12181 aaaaatttgt tagtattttt tgattttagg aattgtttta ttgagtgtat ctactttagg
    12241 aattacagta gatgctgcaa cttattatgg aaatggtgtt tattgtaata ctcaaaaatg
    12301 ttgggtagat tggagtagag ctcgctctga gattgttgat agaggagtaa aagcttacgt
    12361 taatggtttt acaaaagtgc taggtggagt cggtggaaga taaaaaagaa aaactttttg
    12421 acttaattgc gaatgcatat aattcaaagt catataataa tgtagaagta aaagaaggat
    12481 ttcttcaaga tgctaagaaa atacgagagg ataaaccata tcctgaaatt attatatcag
    12541 tgtacaaaca tttatctaaa ttaaaagctg aggaacgc
//
LOCUS       DAZUMK010000079        12122 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000079 DAZUMK010000000
VERSION     DAZUMK010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12122
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.081"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..548)
                     /locus_tag="ACG808_001988"
     CDS             complement(<1..548)
                     /locus_tag="ACG808_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS5047603.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAA"
     gene            complement(587..1705)
                     /locus_tag="ACG808_001989"
     CDS             complement(587..1705)
                     /locus_tag="ACG808_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFS5047604.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(1702..3801)
                     /locus_tag="ACG808_001990"
     CDS             complement(1702..3801)
                     /locus_tag="ACG808_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFS5047605.1"
                     /translation="MTINEVIYSPKHPLFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(3852..4673)
                     /locus_tag="ACG808_001991"
     CDS             complement(3852..4673)
                     /locus_tag="ACG808_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS5047606.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLGCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(4670..5479)
                     /locus_tag="ACG808_001992"
     CDS             complement(4670..5479)
                     /locus_tag="ACG808_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS5047607.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFVMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(5501..5995)
                     /locus_tag="ACG808_001993"
     CDS             complement(5501..5995)
                     /locus_tag="ACG808_001993"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5047608.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6007..6507)
                     /locus_tag="ACG808_001994"
     CDS             complement(6007..6507)
                     /locus_tag="ACG808_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5047609.1"
                     /translation="MIKPMFLKVGTGIAYMKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDYEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQEIKMTILDEVHVVN
                     EEEDFF"
     gene            complement(6572..9190)
                     /locus_tag="ACG808_001995"
     CDS             complement(6572..9190)
                     /locus_tag="ACG808_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS5047610.1"
                     /translation="MKKSAKDRVFDELIRLTGNMSSVTAEKISKNLGISRQNASHYLT
                     RLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQINEAFHSVVGHDGSLREVIQKCIAAV
                     KYPPNGLSVLINGATGVGKSFLATKIFEYAIHEQIIEEDAPFAILNCADYADNPELLS
                     ATLFGYKKGAFTGAEKDTEGLLATANNGYLFLDEVHRLSKENQEKLFVFIDTGNYRPV
                     GENVNWHSAKVRFIFATTEKSEDYLLDTFDRRIQISVMLPTFEDRPIRERLELIQLFF
                     QNEADILQKDIIVSKEALSLMLSHPFSGNIGKLKNIIKISCADVYSRTREEPLTIGKN
                     EILIQLNMLESEVESLPIASLLIKKNQKIKTDRIKPFDYDSILEKITQLINEGNFNEK
                     FSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMFRKVWVSVLGKTYGLTTSGPVAEIISR
                     SYVISPSYKTDLTGINRTLAANLPRTTYLCNQFLEHLPPLSEKYENFLRLLIVVTLSD
                     YVDESIELKGLLLAHGDSTASSIQAVVNQLCGNYVFEALDMPVESNVSEIVESTRNFV
                     KRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGELLLLNNLTTSIALDIGLKMTMNAPFK
                     QIAEDAADKYSINVQYFEGFSQSSNIIISCMSGLGISDKLKDVFRHHLSDESIEVFTK
                     EYRELRELIDQNDETYFDKTKLVITTSDLPRSFSIPNINVYDILDGDGAANLKHILSS
                     EMKPVEFNRLIQELIKFFSLEGIADRLNFLNPTIVMSEVETVISKYENYYHFTLNGRV
                     KLNLYMHTALMMERLFLARTEKAEEPNYDMKPDEDEFYYVSRSIFQSMEMKYNFKVNA
                     YELSLLYELLGPYIQK"
     gene            9673..9888
                     /locus_tag="ACG808_001996"
     CDS             9673..9888
                     /locus_tag="ACG808_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFS5047611.1"
                     /translation="MKKFIIPCIFLFLSILLFSTSILVGSYLDSSGMLIEPAFFCVPL
                     GYLTLGLAFFTALYSFVKQRLLKKYSA"
     gene            10749..11708
                     /locus_tag="ACG808_001997"
                     /pseudo
     CDS             10749..11708
                     /locus_tag="ACG808_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT         3733 a         2669 c         1986 g         3734 t
ORIGIN      
        1 gctgccatcc cctctaaaca atcttggtgg aagggacagt ttcctgaaaa attgtccttt
       61 ggatgtcgct tgatcaacat atgccccatt tccggatgac tgaacccttc cacgaaagtc
      121 ccattgaaca aggcaccacc tccgatacct gttcccacag tgaagtatgc aacacctgct
      181 aaccctttgc cagaaccagc aacatattca ccatagcaag ccgcattgac atctgttgtc
      241 cattcaatcg gaatattgaa atattgcttt agcgttccta caaaattgaa attttgccaa
      301 gcaagttttg gtgttgatgt gatatgaccg aaactcgttg atttgcattg aatatcaatt
      361 ggaccaaagc agccgacccc gatgcctgcc aattgttcct taaaaggttt aaaaaaatca
      421 atgacctgct tcattgtttc aactggagtt gttgtgggaa aactgactct ttccaaaatc
      481 gttaattcct cagtaccgac tgcacagaca aatttagtcc ctccagcttc aattgcccca
      541 tatatcatca taggttcctc ccttcgaata tgtgaaaaac acttcattaa cgcggcaagc
      601 gttctcgatc agtgaaatga cgtttagtat ttcctgaagc ttctgttgaa taccagtaag
      661 cgacactagc aacatcgtct tgccgctcaa ataagcgaac atcatcgttg ccgatttgtt
      721 gtaaagtgac cgataaatct tcggtaaaac agatggggtc ttgaagatgc caacgataaa
      781 gaccatgtcg tggcattgcg tcgtcaccaa aggcatgaac ggcattcaat tttcccgttt
      841 caaaacgatc tcttgtggca tctctatttg attggtaagg ataccctaaa aaggctgttg
      901 aatagttttt cgcatgtgga cgtccgaact catcgtactc atgaaaggcc cacgccccgc
      961 caaaataatc ttctgaacct gttgaggtta cggtcggata ctcactatcc ccatcaatgt
     1021 agaatttaaa ctcgccttcg ccccaccaat aacgttctag gcaggtaagc gcaaggtaag
     1081 tacccacgta atagccttga ccctttaatc gatcaattaa cttataatcc tcctgtaatt
     1141 tggtaatgcg ttcccgatgc caatttgcgt gaaaatatag gctattttct tccaattcgt
     1201 ccaccaaagc gtaatttatc gtataaaaaa atgaggacat atccttcgga tgtaaattct
     1261 cgatcgtaat tttagcggat gtttggaaag gcatttcgaa ataggaatta aagccccctg
     1321 taggattaac cactatcggc aacgagttca cctcgcaacg ctctccaaac ccattacaaa
     1381 agaaatcccc taaaggactt tcaacagcag ggctatccga cccatcccaa taaattctta
     1441 agattaaatc acgtaaaaca aaactgccaa attctgttcg atcacgaatc gtcatccaca
     1501 tatgacgaat tattcccgtt ccagtaatat ccgcaatcac agtttcttca ccagaagtca
     1561 gcggaattgc gccacgacct ttgcggcttg gtcctagctc gcttggcaac atcgcagctt
     1621 tacctttctc gcctgttgga ttttcgggag tgattgctcg actctggatt tttttaaatt
     1681 gggtaatatt ttttagtata ctcatctttt tctcctttga atgtgaatcg tttttccctt
     1741 ttctacgtga agaatttttt tcgtatcact tattttcaat ggcttttctg acaaatttga
     1801 aatactcaac tcggattgcg tacattcaat cgaaatgaag gcttgaccga acttcaaatt
     1861 cttccaagaa aacgttaaat tatcaacagg atcaatttgc aatactcctt ttttgaattt
     1921 caatccgaaa tattgactga tcattaggct gacaaaaaga cccgatgccc agccgcattt
     1981 gccgcagcca aagtctcgca cgacttctcc ttgttgagcc tctaagcgat acggccacca
     2041 ccaccacgaa ccgtctaaat ctgccaaaca aattagttct tctaatcggg ttacgaaagc
     2101 ttgagggttg tgcagctcgc tcattaaaac agtgataaag ccaggaaaag tggcgccgga
     2161 ttctaatccc caagtgatgc ctttgatttc ctcgctatag gttggattga aagaactagc
     2221 agaaaaggcc atggtgttct gatataaatg atctttatta tctaaaaatt gataaaaagg
     2281 catcaacgta gtgtctgatt cttctccatc atgcatcaat aaattaattt ttccatccac
     2341 aatcacatca cttaaaaaga ttaacccttg ctcaagaatc ggtttttgat agtgcttcac
     2401 tgaataggtt gttttctcct catcaccgat tccctccaaa aattgctcac caaaaggacc
     2461 atcgatagtc atttgtttac aaattgattc ctttagttta caggcaatgt ttttgtatcg
     2521 cttgctaaga ctcagttgtt ctagggcttc aaaaatttct gcaagtccaa tacaggcctt
     2581 ccaaagacaa agattgctgc cggtatgata ctttccaagt gcaaaggcgt ctgataacca
     2641 aacagatgga aaaagcattg gttcccctga ccgacgttga gctaatatgt cctcaataat
     2701 tcttgaagca ttcagtagaa cctgcggaca ccgctcaaag aattttgtat ccccagtatg
     2761 ttcaaaataa agactagcca gtaacgcact ggataacgaa ttactcagag aatgattgat
     2821 acctcctgta aattttgaac caggaaactt aacgccatat tgatcaaacc atagaaccgt
     2881 tttttgacat aactccggtt caaacatggt gaatggcaat gatgcgtaat acatatcctt
     2941 attccaaatt cgattagtag caggatagct gccccaattg ctgccgacga cctgtccctg
     3001 tcgattcata ccgaaactag aaaagctttg ataaagtgca cgacgataga gatggacaac
     3061 tctatcgttt ggcattgaca gctgcccaaa tagctgagca aaatagttca atgtttcctt
     3121 cagccatttt tcgatatcag attcctgaaa agtcattact tcttcaaaag catttgggtc
     3181 cacaaaagca atggcgaagc ttgccacttc ctgttgtgcg atcgtgtcct tatttccttt
     3241 ttgagaaatc aacacatttt gctgatccga atacttctct tgatacgaag gcacatcttt
     3301 cactgaaact tgaaggcttt cgtgtgtttc attcacaatg taaacctgtt gaacaagcat
     3361 acttaaacgc ttattagcca gtatgggagc aaaggatact acaatgacct taaacgtcga
     3421 ataatgatga atatatctag gcaaacagtc ttgaatcaca tcgacttcaa tacgatgatc
     3481 cgattctgac aaatggtaga caatcccgtt tacttcaacc tttaaggata acgaattggt
     3541 ttgagagagc tgtttattga cccaaacacc tggtttttcc tcagttagta aattttctcg
     3601 ataaaaggtc aacgtttgaa tcgtcccgca aacatcaagg tttgctaatg tcttttgatt
     3661 acttacatcg aaggtcaatc cctttacatc aggaatattc cgatagtagc tgtattcgtt
     3721 tgaagtagtc acatttccgt cgtttaactc agcattaaat aatgggtgtt tgggtgaata
     3781 aattacttca ttaatagtca tcttttccct ccttgctaaa aaaactgcag cctagtagct
     3841 gcagcttcct actatgcgat tccaagtaat gacaataaaa ttcccaagac gaccattcca
     3901 aaaataattt tgatgacgcc gactttttta tctagtaatt tgaaacagcc caatgttacc
     3961 aataacggta caattccttt aaaaatacta tcgaggattt cttggacatt cattagttct
     4021 tcgcccttca ttgaaaagct caaaacggta ttgaatttga ccatactgat cgtcattgcg
     4081 cctaccatga tcagacccaa gatactagca gcttttgtca gaatttcaat taacccgttc
     4141 gcatacagct tttcaatata tttagagcct aatgaatagc ctaaaacgcc gccatagtat
     4201 cgcatcaaat aattaggtat attgaacata actaaaaaga taattggtgc caaaatattt
     4261 ccggcattgg ctaagttgat cgctagtccc gcggtaacga cacgccaaac tccccagaac
     4321 aatgaatcgc caattccagc caatggtccc atcaaactcg ttttgatcgc gttgatactt
     4381 tccacgtcaa aaccagcttt ttcagaattt tccttctcca tcgatgccgc aatccccatg
     4441 acaaaggacg cgcaaggaat ggtcgtcgaa aaatactgtg tatgacgttc cagcgccgca
     4501 gctcgatctt cttcactttt gtagaaatga ttaatgaagg gcatcataga ataggcgaaa
     4561 ccaccgccgc ccatcgtcac cggattcacc gaagcataca tagtcatcga gcgccaaaac
     4621 atcttccgaa ccaatttttt ctcatcttta gaaattccat cagctaattt cattgaaaga
     4681 aatcctcctc ttcttggtca agcgttgcat ttacagcagc atcgctttga actgcatgaa
     4741 cagatatatt ttttagttgt agatctcttt gggctataac aattgcaata atcgttccaa
     4801 ctgcggcaat tgcaatcaat ggtaactcga gatacgccat aacaacggcc ccaaagaaaa
     4861 accacacagc aatattttta ctccaaagca tccgtaacag catagccatc ccaactgccg
     4921 gcagcagatt tccagcgact gttaatccgt tcatgatgac ctctggaata ttatccaaga
     4981 cgcttttgac aacatctgct ccaaataaaa cggcaaagaa tggaataaga gcataaagcg
     5041 catagtggac aaaccacata acgaagtgca gattgatgat tcctttttga tcaccagatt
     5101 ttgcaaattt gaaaaattta gcagccatcg gtccacatac aaaagaaaaa atcatattca
     5161 ttaactgtaa tcctaatact gctagtggaa agacaatggg aataatcgca tccacgtttc
     5221 ctcccatttt gattgcgaac gctgtagcca acccagcagc caaagaaggc tccgccgtac
     5281 tagagattcc gatattgacg acacccataa aaatggcttc tagtgacgcc ccgacaatca
     5341 accccgtctg caaatccccc atcaacgctc ctgctaacgg agcaacaaca atcggacgac
     5401 tcagcatgga catcccgaac atttcatgtc cagcaactcc aataaaaacg gccaaagcga
     5461 ctacaaatgc atcatacata atttttctcc ttcctttttt ctaaaaatca ctcaaaatag
     5521 agatagctga acgtttttcc atcgccggtg taggctgaaa aacggtatct ggataaagtt
     5581 caactaattc ttttaagtct tgaatttctg acgtcgttaa catcacttcc tttgataagg
     5641 ttttcttatc ttccgatggc ggtgtcattt tcccagcatt ccccacgttc attaattcga
     5701 tttccgttgg gaaagctttc gcaactttta ccgcatcttt gacattaggg actaaaacca
     5761 tcaagcgcat gtccttcgcc cgcggatctt ttaacaaatg aatcgcgtca tcaactgttt
     5821 taataatgac tttgacattc gatggtgcag ccatcttcaa tgtcatcttt tgaatatcgt
     5881 tggtagccgc ctcatcgctg gctactacta acccggttaa atttagactc tttgtccaag
     5941 tcatcgctac ttgtccatga attaatcgtt catcaattcg cactgcttta atcatttttc
     6001 ttcctcctaa aaaaaatctt cctcttcatt tactacatgt acctcatcaa gaatggtcat
     6061 ctttatttcc tgttggcatt tatttaccag ttcctgaata tcctcctttg ttaattcctt
     6121 ggttgccaat actatttcga gcagaattgg cagattaaag ccagacaaaa tagttacagc
     6181 aatctctttc tccaaaaagg ttttggtaat cttctgattg acacttccgc caaataaatc
     6241 ggtaaaaaca aaaatctgat cctgctcgtt cacctggttt acaaatacat tcaactcctg
     6301 ttcataatca cactcatcca cataagcatt aatcaccgta atctcttgaa ccttccccgt
     6361 cagtaattga atactgctaa gaaagcctga agctaagtga ccgtgagtcg ctattagaat
     6421 tttcttcata tggttaattc ctttctttct gttcactttc atataagcaa ttcctgtgcc
     6481 aacttttaaa aacattggtt taatcacgtt aattcacata aaaaaaagtg tctaacctga
     6541 aaaaatcaga ttagacactt ttttagacac tttatttttg aatatacggt cctaataatt
     6601 catagagcag cgaaagctca taagcattga ctttaaaatt atatttcatc tccattgatt
     6661 gaaaaatact tctcgaaaca taataaaatt catcttcatc tggtttcata tcataatttg
     6721 gttcttctgc tttttctgtc cgcgccaaga ataaacgttc catcatcaat gccgtatgca
     6781 tataaagatt tagttttacg cggccattta aagtgaaatg atagtaattt tcatatttag
     6841 aaatgactgt ttccacctca ctcatcacga ttgttgggtt cagaaagttc aaacggtctg
     6901 cgattccttc caatgaaaag aattttatca gctcctgaat caaacggtta aattctaccg
     6961 gcttcatctc agaactcaaa atatgcttca aattggctgc tccatcacca tctaaaatat
     7021 catatacatt gatattagga attgaaaagg aacgtggaag atccgaagtc gtgatgacca
     7081 attttgtctt gtcaaaataa gtttcatcat tttggtcgat taattcacgt agttctctgt
     7141 attccttcgt gaaaacttcg atggactcat ccgataaatg atgacgaaaa acatccttca
     7201 gtttatccga gatacccaat cccgacatgc aggaaatgat gatgttgcta ctttgagaaa
     7261 agccttcaaa gtattgaaca ttgattgaat acttgtctgc tgcatcctcc gcaatctgtt
     7321 taaatggcgc attcatcgtc atcttcaggc caatatcaag tgcaatggag gtcgtcaaat
     7381 tattcagaag aagtaattct ccttttaaat cgttcttgat ctgtgtgtag atttgattta
     7441 aagagcccat atctacgatc agcactagac tatctgtatt cacttgccgt ttcacgaaat
     7501 tccttgtcga ttcgacaatc tctgacacat tactctctac tggcatatcc aacgcttcaa
     7561 aaacataatt gccgcataat tgattcacta ctgcttgaat gctgctagcc gtactatctc
     7621 cgtgagcaag caacaggccc ttcagctcga tggattcatc tacataatca gataaagtca
     7681 cgacaataag cagtcttaga aagttctcgt acttttctga gagcggtggt aaatgctcca
     7741 agaactgatt acataagtaa gtcgtccgag gtagatttgc agctaatgta cgatttatcc
     7801 cggttaagtc tgtcttatag cttggggaaa tgacatagga tctgctaata atttctgcaa
     7861 caggtccact cgtcgtcaat ccgtacgttt ttcctaaaac ggatacccag acctttctaa
     7921 acatctgaat catcaatttt ctactcattg ataaaaaaga atccttatca atgtgatgca
     7981 ccaaatgctg tacttcattc tttatcgaag aaaacttttc attgaaatta ccttcattta
     8041 tcagctgagt aattttttcc aaaatcgaat cataatcaaa cggtttgatt cgatccgttt
     8101 ttatcttttg atttttcttt atcaataaac tagcaatcgg caagctttct acttcggatt
     8161 ccagcatatt taactgaatc aagatctcat ttttcccaat cgttaatggc tcttcccgtg
     8221 ttcgactgta cacatccgca caactgattt taatgatatt cttcagctta ccaatattcc
     8281 cactaaaggg atgagataac attagagaca aagcttcttt tgaaacaata atatcttttt
     8341 gtaaaatatc cgcttcattt tgaaaaaata attgaatcag ctctaaccgt tctcgaatgg
     8401 gacgatcttc aaaagttggc aacataacac tgatttgaat acgtcgatca aaggtgtcta
     8461 ataaataatc ttcacttttt tccgtcgtgg cgaagataaa acgaacctta gcagaatgcc
     8521 agtttacatt ttcaccgact ggacgataat ttccagtatc aataaaaaca aagagctttt
     8581 cttgattttc tttcgacaaa cgatggacct catcaagaaa aagatagcca ttgttggctg
     8641 ttgccaataa cccctctgta tccttctcag cccccgtaaa ggcccctttc ttgtatccaa
     8701 acagtgttgc cgaaagcagt tcgggattgt ccgcataatc ggcacaattt aaaatagcaa
     8761 atggtgcatc ttcttcaata atttgctcgt ggatagcgta ttcaaaaatt tttgtggcta
     8821 aaaaactttt tccaaccccc gtggctccat ttattaatac actgagtccg ttaggcggat
     8881 atttgacagc tgcaatacat ttttgaatga cctctctcag acttccgtca tgacccacca
     8941 cagaatgaaa ggcttcgttt atttgctccg tattatctac aaccgcatat tcatctaaag
     9001 gcttccacaa aacaggcttt cccggtaatt tctcgacttt tttatcttcc actaatctcg
     9061 tcaaataatg gctggcattc tgacgagaga tacctagatt cttcgaaatt ttctcggcag
     9121 tgacagaaga catattccct gttagtcgta tcagctcatc aaaaactcga tctttagcgc
     9181 tctttttcat tttcccctcc agttcaacgt gtctagaagc ataataaact atcggctaca
     9241 atatgtctat tctatatttc tgttccaatt ttactttgaa acaaaaataa tcgagcttaa
     9301 actgatatta gttataaaac tatagttaga ttaccaatct tcctctcata caataaactt
     9361 ctctcacctg catccaacct acttatgtga aagaataact gccgattatt tatctttttt
     9421 acagttactt tcgaacataa aaaaatcacc ttcaaatact agaaaaacta ctatttgatg
     9481 gtggtttaga acaatttccg ttgattcagt atcgtctggt cccacatcct ctgtctcatt
     9541 aatcacaata gcatactcca aaggataaag ctggttgata tctcaatgcc tttcacacct
     9601 atcaattttg tcacacttat cttttcctag gttctattct atataatcta gaaaaacaaa
     9661 gaaggagaca ccatgaaaaa attcattatt ccttgtatat ttcttttctt gagtattttg
     9721 ttattttcaa cttccatcct agtcggttct tacctcgatt caagcggaat gctgattgaa
     9781 ccagcgttct tttgtgttcc gttgggctac ttaacattag gacttgcttt ctttactgcc
     9841 ttatacagtt tcgtcaaaca aagattattg aagaaatact cggcttgatt tgcacgtaaa
     9901 tcctctgttg aagaaaagtt agttttttca acaacgaacc ttcgagtatt aagaatggat
     9961 gttgatgtag acatcaaacg tctgcataaa cattcatttt attcctccat taccaacaat
    10021 tattcccaat cgccaaaaga agcttttttc ggaattacct tctccatttt ttcacctgct
    10081 ggttatattg tcttttgtaa taccgatagt cctcttcaat cattttcaat atgcgaaaag
    10141 aaccaatttt aacatggcac tcaaatcaca attcctaggg cggccaaaaa gatacggctc
    10201 gttgatttca agggtttcaa cgagttcatt gacaaaccca gcggtgctat cttcttttgg
    10261 aataagaagt tgtgatatct aaagtgagtt gattcatgtt ataatttgta tgcatgattt
    10321 tgatcctttc aagggttgaa tgatttagag tgcgagtatc ctctcgtact cttttatttt
    10381 acctaaaaag ctccagaaat gacgaatgac tcgtcatttc tagagctttt ttcagagaat
    10441 tatgtcacaa tctctttttg ttatattgat attctatatc tttttttatt ggttttattc
    10501 ccttgttcgt tatagactaa aatccttctt tcagcattat tcaaatcaaa tggcaacata
    10561 atagagaaat ttatagtatg tataatcatc tgaattcaat caaatcaacc ggagtcatat
    10621 acggagtcaa actatcccac tgagagttta ttaaacgcag attgtaaaat taagctagac
    10681 aactaaaaaa gtcatttgtg ctacactcaa aatagttccg accaaagaat tataaggagt
    10741 gaagacaaat gacctatacc catcttacaa caaatgaact cgtgatgata gaagcctatt
    10801 tcaatatgca tcaatctgtt accgtagtcg ctaagacctc aaatcgttcc agacagacca
    10861 tctacaatgt gtacaacttc ctgaaacaag gccaatctat ccatgactat taccaacaat
    10921 atcaaaaaaa taaaacacgg tgcggccgac gacccatttg tttgccagca gaacaagatg
    10981 cgtatatccg aaacaaggtg gcgcaaggat ggacacccga tgtgattatc ggtcgtgctg
    11041 agttttcaat ttcatgttct gttcgaaccc tttatcgcaa gtttaaaaca aactaatttg
    11101 attgctcgaa actacccatg aaaggaaaac ggaaacccaa cgggcataaa gaacgtcgag
    11161 ggaaacaagc cttcaaacgg aatatttcag aacgtctcac ggactatcct gaatttcaac
    11221 aggaatttgg acatttggaa ggcgacacta ttgtgggtgt ccatcataaa agtgctgtca
    11281 tcacgctggt tgagcgcttg tcaaaagtga ttattacttt aaaacccgaa ggacggaaag
    11341 cactagatat tgaaaaagtg acaactgact ggctacaaaa gcttccccga aacttgttca
    11401 aatccattac ctttgattgc ggaaaagaat tttcgaattg gaaaaacatg agtaatgcta
    11461 acgatatttc tatttatttt gcagatcctg gaacgctgtc acaacgggga ttaaatgaac
    11521 attctaatgg attgttacgt aaagacggac ttcctaatac aatggatttc aatcaggttg
    11581 accaaaaatt tgtggcggcg gttgcctcca aaagaaatca tataccaagg aaatctttaa
    11641 attatcgaac atcgttagaa gtatttttga gttacataag tgaggagcaa ttgtctaact
    11701 taatttgaca tttagaaaat aataaaccat atcccatcta ttcaaataat attttaccga
    11761 ttgaaagact tgctaatcaa agcagaatag tttatataga taaattattt tctttaaact
    11821 tatactcatt tttagatatt ttataagaat acttcatgtc ttcgccagta atataaagaa
    11881 catctgtctc tccatttgga cttgggttat ctattttggg tgtaacatac tatacataca
    11941 accgatacat aaaagatttg acatctggac aaaacacaat gctttacttc atttttatag
    12001 gctttgttta caccatcaca aaaatcacct cctaatttac aaattcatta aacaaacaga
    12061 aataaaaaat caccacgata gccgcgttca aaaaaattta caaaagagaa ttttagttta
    12121 ag
//
LOCUS       DAZUMK010000080        12117 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000080 DAZUMK010000000
VERSION     DAZUMK010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.082"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..733
                     /locus_tag="ACG808_001998"
     CDS             <1..733
                     /locus_tag="ACG808_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFS5047612.1"
                     /translation="LSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEPKSIPTTLN
                     VALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGMNKINAAYS
                     YGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSLDLDGVYIN
                     KGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFKLPKAVGKL
                     LGSIQTNLPESVLLDCGMDFLKDIC"
     gene            791..2830
                     /locus_tag="ACG808_001999"
     CDS             791..2830
                     /locus_tag="ACG808_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFS5047613.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFQTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLINPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(2939..4090)
                     /locus_tag="ACG808_002000"
     CDS             complement(2939..4090)
                     /locus_tag="ACG808_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFS5047614.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4244..4774)
                     /locus_tag="ACG808_002001"
     CDS             complement(4244..4774)
                     /locus_tag="ACG808_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFS5047615.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(4873..5223)
                     /locus_tag="ACG808_002002"
     CDS             complement(4873..5223)
                     /locus_tag="ACG808_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFS5047616.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5380..6909)
                     /gene="ahpF"
                     /locus_tag="ACG808_002003"
     CDS             complement(5380..6909)
                     /gene="ahpF"
                     /locus_tag="ACG808_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFS5047617.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(6919..7482)
                     /gene="ahpC"
                     /locus_tag="ACG808_002004"
     CDS             complement(6919..7482)
                     /gene="ahpC"
                     /locus_tag="ACG808_002004"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFS5047618.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7626..8288)
                     /locus_tag="ACG808_002005"
     CDS             complement(7626..8288)
                     /locus_tag="ACG808_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5047619.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8472..10118
                     /locus_tag="ACG808_002006"
     CDS             8472..10118
                     /locus_tag="ACG808_002006"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFS5047620.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10109..11191
                     /gene="uxuA"
                     /locus_tag="ACG808_002007"
     CDS             10109..11191
                     /gene="uxuA"
                     /locus_tag="ACG808_002007"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFS5047621.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(11299..>12117)
                     /locus_tag="ACG808_002008"
     CDS             complement(11299..>12117)
                     /locus_tag="ACG808_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFS5047622.1"
                     /translation="YIDFFIDPSVDILRPNLPICREQRILVNILARLSITKIESEETS
                     LEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAFARNKLYNGFLNFN
                     QPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILEKENVQWMVEDLVH
                     LYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQNDFMENCNIVISDCP
                     LKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQSKKENQVTS"
BASE COUNT         4050 a         2248 c         2270 g         3549 t
ORIGIN      
        1 attatctatt ttgttactgc ttacattagt agttggctat ttttcgattg agtttatcca
       61 tggattttcg tctgcaaaac agagttcaac tgtgaaaaag gtagagccga aaagtatccc
      121 taccacacta aatgtggctt tgattggttc agatgctcgt tcgaaagacg aaaacggtcg
      181 ctcggattca cttctggttg cacaatacaa ccagaaaaca caacaagcaa aactgatctc
      241 tatcatgaga gattcatatg tcgatattcc aggttacgga atgaataaga taaatgcagc
      301 ttactcttat gggggagttg atttattaaa tcaaacatta aaggaaaatt tcaaatttga
      361 agctccctat tatgcaagta tcacatttca agattttatc gattgcgtta atgaactgtt
      421 tcctggtgga gtcaagatcg atgcagaaaa atctctagat ttagatggcg tatatataaa
      481 caaaggagag caagtgatgg atggtaatac tttactccag tatgctcgat ttcgggaaga
      541 caaagaagga gacttcggga ggatcagaag gcagcagcaa gtgatcaaag ctatctctca
      601 gcagctgaaa gacgtaactt caatattcaa gctacccaaa gcggttggga aattacttgg
      661 tagtatccaa acgaaccttc cagaaagtgt gcttctggat tgtggtatgg atttcctcaa
      721 agacatatgt tagaataaac aggtataaat agtgaaataa aggaatgaca agcaagagaa
      781 ggaggaaaaa atgaaaagaa gtgacaagca cggcaaaaat cgaacaggcg cttatattgc
      841 cggcgcagtg atcttaatag cagctgcggg tggcggttat ttttactacc agcactatca
      901 agaaacccaa gcagtagaag ctggagaaaa gacagttgag caatttgtcc aagctttgaa
      961 caagggagat tataacaaag ctgcagaaat gacatcaaaa aaggcggcaa ataaaagtgc
     1021 attatctgaa aaagagatct tagataaata ccagaatata tacggtgctg ctgatgtcaa
     1081 aggacttcag atatcgaatc taaaagtaga taaaaaagac gattctactt acagtttttc
     1141 atataaagca aagatgaata cctcattagg tgaattgaaa gatctttctt ataaaggaac
     1201 attagacaga aatgatggtc aaaccacgat caactggcaa cccaacttgg tttttccaga
     1261 aatggaagga aatgacaaag taagcctgac cacacaagaa gcagcaagag ggaatattat
     1321 agatcgaaat ggtgaaccat tagcaacaac aggcaaacta aaacaattag gagtcgttcc
     1381 aagtaaactt ggagatggag gcgaaaaaac agccaatatc aaagcgattg cttcctcctt
     1441 cgacttaaca gaagatgcta tcaatcaggc gatttcacaa agctgggtac aacccgatta
     1501 ctttgtccca ttgaaaatca ttgatggagc aacgccagaa cttccagctg gagctaccat
     1561 tcaagaagta gacggcagat attatccttt gggtgaagca gctgctcagc tgattggtta
     1621 cgtgggagat atcacagcag aagatattga taagaatcca gaattaagca gtaatggaaa
     1681 aatcggtcgc tcaggtttgg agatggcttt tgataaggat cttcgtggga caacaggtgg
     1741 gaaattaagc attacagatg cagacggtgt agagaaaaaa gttctgatcg aacatgaagt
     1801 tcaaaatggc aaagatatca aattgacaat cgatgcgaag gcacaaaaaa cagcttttga
     1861 cagtctagga ggaaaagctg gatcaactgt tgcgacaacg ccaaaaaccg gtgatcttct
     1921 tgcgcttgct agctctccaa gctatgatcc aaacaaaatg acaaacggga tctcacaaga
     1981 agattacaaa gcttatgaag aaaatcctga acatccattc atcagccgat ttgcgacagg
     2041 ttatgctcct ggatctacgt ttaaaataat tacagcagca atcggtctcg acaacggcac
     2101 tatcgatcca aatgaagtgt tgacgatcaa cgggcttaaa tggcaaaaag acagttcttg
     2161 gggatctttt caaacaacgc gtgtcagtag tgatgtatca caagtagact taaaaactgc
     2221 tttgatctat tccgataata tatatgcggc acaagaaacg ttgaaaatgg gtgagaaaaa
     2281 atttcgtaca ggcttagata aattcatttt tggtgaagac cttgatttgc caattagtat
     2341 gaatccagca caaatttcta atgaagatag ctttaactca gatatcttgc tagctgatac
     2401 tggatatgga cagggcgaac ttctaattaa tcctatccag caagcagcaa tgtattctgt
     2461 ttttaccaac aatggcacac ttgtctatcc taaattgatt gcagataaag agacaaaaga
     2521 taagaagaat gtcatcagcg aaacagcagt acaaacgatc gtgccagatc tgagagaagt
     2581 tgtgcaagat gtaaatggta cagcacattc tctttctgct ttagggattc cgttggcagc
     2641 gaaaactggt acagcggaaa tcaaagaaaa acaggatgta aaggggcaag agaacagttt
     2701 cttgtttgct ttcaatcttg ataaccaagg ttatatgatg gtcagcatgt tggaaaataa
     2761 agaagatgat gattcagcaa ccaaacgagc atcggaacta ttgcaatacc tcaaccaaaa
     2821 ttatcaataa aaaattgctt tttaagtaca gtaaaaggac tgtggcgtta tcgtcacagt
     2881 ccttttttca aataaacggt tttcacgatg tctctttcac aacttctttt tgatttactc
     2941 acacttcata aacttttttt gtaaaatatt tgagaacaag tggagaaata agtgtactta
     3001 ataaaatcac gataactaaa ggtgaatagt actgtggttc gatcaattga ctttgctgtc
     3061 caatttgtaa aatgatcaaa gccatttctc cacgcgaaat cataccagca ccgaccatca
     3121 atgaactatg ttgagaaaaa ccggcaatct gagcaccaaa ataacctcca agcaatttgg
     3181 tcaaaatcgc tacaagcgtc aatatagaaa taaagagtag ttgttcacta aagtcagaaa
     3241 aatcaacttc taatcctaca ctgacaaaaa atactggtat aaatactgca tatcctaaag
     3301 cctctacatt gtggtatact tcttctttaa cctttgtctg actgacagct atcccagcaa
     3361 aaaaagcacc aatgacagag ctaagcccaa caagatccgc tacgtaagac attcctaaac
     3421 aaataactag cgatacgatg ataatagaag aatttgcgta cactttttct gcaaatgaaa
     3481 gtaggaaagg cgcaatccat ttgactaata agaaaataaa aacaaaataa attagctgtt
     3541 caatcaaaac aagcggtaaa gaagcattgc tagaagcttc ccccgttaga aaagataggc
     3601 tgaaacttag aactaaaacg actaaaatgt catctaccac agaagcaccc aaaattgttg
     3661 atccttcttt tgtattcact acgtttagtt cttttaatac ttctactgaa atactgacag
     3721 aagttgccgc aagaataatc ccaaagaaaa tcgcttcatt tcccgaaact tggaatgctt
     3781 tgcctgtcaa ccagccaaaa acaaccggga agatgatccc aagcaaagca acgaacatac
     3841 caggtcggaa atattttttc aaaagagata aatcactttc cagaccagct aaaaacatta
     3901 ataagatcac accaatctcc gaaaagtcat ggactagaat atcaggtttg acccaattca
     3961 atccagcttg gcctagcaaa acaccaacaa gtaattgtcc aatcactgct gggataccta
     4021 atctccgcga gatatgtgag ccaatcgttg ttgaaatcaa tatcaggcat aaaataccaa
     4081 taaattccat tctcatcatt cctttgtata aaatatagcc cgaacttagc ataacataca
     4141 ttattgaaaa aataggaaaa tatactcaca aaaaaaccaa caatccaaaa gatttttttg
     4201 gattgttggt ttttatacgg tctattttaa taactaaaaa tgattaacgt atagtcattt
     4261 ttcttattct gatgaaattg ataatctcct ggatgcccat gaaaatcaaa ataaagccga
     4321 atatttggaa tagaagcatc aaacttttga atggattgaa caataagacg aatccgccaa
     4381 tcaacaacag aatactacta taaacactcc aacttttagc ttttgtattc atgctgaaga
     4441 acaactggaa caagctgttg atgataacag ctaatccaag aagaatcggc aggatagaaa
     4501 cgattgcagg tgcaaaagca tagacgatca gagctaagat caggaaaaaa agaccactga
     4561 acagaccgat actccaagaa cctgttttcc ttttaaaacg gtaagcctca aaaagattca
     4621 acaacccaag caaaacaaga taggcggtga tcagataacc gataaaatga aaaacagcag
     4681 aagggtttag cacaatcgca atcccagaaa taatataaac agcagctctt aataaggcgt
     4741 atttttgaat attttgttta atcgtctcaa acatataaga aaacctcctt tgacttattt
     4801 taacgtgaac tagtcatttt accaaattaa atgttacaaa atgagcgaag gagaagaata
     4861 tctctggaaa aatcaacttc tgaggatctt ttccattttt ttgccttttg ctagctcatc
     4921 gatcatttta tctaaataac gaatttcttt catcaagtta tcctcgatgt cttctactcg
     4981 tacaccacaa acaacacctg taatcaagga acgtgaagga ttggggacag gggcttccgc
     5041 aaaaaattgt tcgaaagtca cttgtttttg tagttgatat tctagttctg tttgggaata
     5101 acccgtcagc caacaaatga tttcatctac ttcctctttt atacgatctt ttctctccgc
     5161 ttttgctaca tacataggat aaacgcttgc aaaacttgtc gtaaaaatac gattttgcgc
     5221 cataatcagt cacctttctt tttttcaaat tatttactac ttatagtata taagaaaaag
     5281 agaagaaaaa gcgattattc aaaaaaacaa aaaggattgt gacaaaattc ttgtcaccat
     5341 ccttttttcc gctctctgac tttttacaat cttcatagct caagtgcgca tcagataatc
     5401 gaatgctcct aaagctgcag tagctcctga acccattgaa acaatgattt gtttgtatgg
     5461 actatctgta caatcaccag ctgcgaaaac gccggggata ttcgttgcgc catttttctc
     5521 ggtcactatt tcatttcttg gattcatttc gatcgttcca tctagccaat ccgtattagg
     5581 gagtaagccg actagaatga atactcctga aacagctagt gtatgcatct ccatcgtacg
     5641 gcgatcgata taggagagag attctacctg attcgtaccg ctgatttctt tcgtcgctgc
     5701 atgtttgata acagtgacat tcgcaagaga gactagttta tcctgtaaga tttgatctgc
     5761 ttttaattct ggtaagaatt ctagaacata tacatgtttt gctaacccag ccaaatcgat
     5821 tgctgcttca acaccagaat taccaccacc gataacagca atctcttttc ctttaaataa
     5881 agggccgtca cagtgtgggc aataagcaat tcctttattt ttaaactctt tttcgcctgg
     5941 tacattgata gaacgccagt gcgcacctgt cgcgatgatg gcggtttttg ctttaagtga
     6001 tataccgtta gctaagccaa tattaataaa atgttgctct ttatttaagg aaacggcctg
     6061 ttgattcatc ataatgtcga tagggtaact acgcacatga ctttctactt gctgcatcaa
     6121 ttttggacct tctgtgtaag aggtaccaat catattttca atccctagtg tttcaacgac
     6181 ttgtccgccg aaagaatcag cgacaagtcc tgtacggatt cctttacgtg ctgcatagat
     6241 agctgcacta ctagctgctg gtccaccgcc aattactaat acatcataag gttctttttc
     6301 aaacaactct tcttcaatca aagggccagt gattttttca acaatttgtt cgatcgtcat
     6361 gcgtccgcta ctaaattctt ctccatttaa aaacacagtt ggcactgcca tgattttttt
     6421 atcatcgatt tctttttgga acatcccacc ttcaatcatc gtatgagtaa tatttggatt
     6481 taatacagct aatatattca atgcctgtac aacgtcgggg caattatgac aagtcaagct
     6541 aacataactg tcaaaatgca atgtttgtgt tatttttttg attttaagac tcaaagattc
     6601 actgattttc ggtgcccgtc cactaatttg taacatagcc agtaaaaaag aggaaaattc
     6661 atgaccgagg gggataccag caaaaacgat tccactttct ttgccaactg gattgataga
     6721 aaaactaggt gtgcgcatcg aatctctttt ttcccaagag attttaggag acatttctgc
     6781 tatttccttc gtaaattcag ctagtttttg actattttca ctattatcca aactaacaga
     6841 aaaaacaata ggagattcaa tcagtgctaa atattgttcg agattttgtt tagtttcttt
     6901 gtctaacata tccaccacct aaatctttcc aacaagatca aaacttggtt tcaatgtttc
     6961 gccactttct ttccattttg ccggacaaac ttcacctggg tgggtacgta catattgagc
     7021 agcgcgaatc ttatcaatca gtgcactagc gtcacgaccg attccatctg cattgatttc
     7081 catagcttga acgattccgt ctggatcaat aataaatgtt cctcgctgag ctaatccttg
     7141 ctcttcatcc aaaacatcaa aaatacgaga aatctgatga gacggatcac cgatcatcac
     7201 gtattcgatt ttaccgatag catctgatgt atcatgccat gctttgtgag taaagtgggt
     7261 atcggtagag caagaaaaga cttcaacacc taaagatttc aatgtactgt attgttcttg
     7321 taagtcttct aattcagtag gacaaacaaa agtaaaatca gctggataga agcaaagaat
     7381 actccagttc cccataagat ctttttcact gacttctaaa aattctcctt gatgataagc
     7441 atgtgcatta aattcttcga tttttttccc aatcaatgac atagtaaaac ctccaataat
     7501 aattatttat ttataattat tgtaatttag ttggagttgc aaagtcaatg gatattttgt
     7561 tatttaataa agattactca aaaacgaagg ttaattataa aaatagaaga ggtcgcaaga
     7621 aaatgctact gctggaaata ctcgggataa cgttgagtaa cagtttcctg ttctttcagc
     7681 atcatgtgta agtgcaatcc cgtttgaaat cgagcttctt ctggtttttt atcaactaaa
     7741 gccttgagta aatcataatg ttgttccatg atcgtttccc atggaagccc ctgaatggtc
     7801 actcgtaacc agcggaaacg ttctaaatgg gtattatggt catctaacca gttccaaact
     7861 tcttttcttc ctgcaatttc gaaacaagct tcatgaaata aattatctgc acggaaaaaa
     7921 gcgcgttcgt catttatttt gactgctttt tcttgttctt ctaaaatcgt ttctagcact
     7981 attcgtgttt gtgtgtccaa tttggaacaa cattcaatca tgatttgctc ttccaactgt
     8041 tctcggacaa agcgagcatt ttctgcagaa acaagattaa ttttggaaac atacgtacca
     8101 ctttgaggta tcgtatacac gagttcttgc tgacgtagtt gaatcaatgc ttcgcgaatg
     8161 ggagtccggc cgatagcaag atgttcctcc agtgcctttt cagatatttt ccttccaggt
     8221 tctaagtcag catagataat ttgtttacga atcgcttgat aagcttgtga ttgcaagctg
     8281 cttttcatct ttattcctcc aaaatcattt ctatgaacgt agtataacag aagaggtctg
     8341 aaaaacaatc tggtatgtca cattaactag taaatttgta taaatagtaa aattttggtt
     8401 gacatagcga tttttattgt ataaaattca cttgaataca aaaactaata tgttagttta
     8461 ggaggggaaa aatgattgat ttaaacaact ggaggaactt tatccctgaa ttatctcaaa
     8521 aagaagtaga aatacctcat tttgaccgtg catcattaag agaaaatgga caaaaaaatc
     8581 ctgtctggat ccatttcggt ggcggaaatc tttatcgagg atttcatgca gaaatcgctc
     8641 aacgtttagc ggatctaggc gaattgtcca ctggtgtcat tgtttgtgaa acattcgatg
     8701 aagaaataat tgaaagggct taccaagatt ttgatgattt gattcttgaa gtggtcatgc
     8761 caagcagtgg agagttcgtc aaacgtatat tagctgcgac agctgacagt ctgtattgtc
     8821 atccagattg ttctgaacac tttgataaga tgattcgaca ctttgaaaat cctagtttac
     8881 aattagttac ttttgctatt actgaaaaag gttatcgatt gaaagataca aataataaat
     8941 atataggacc gattgcagtc gatataaaaa atggtccaaa gaagccatca catacaatga
     9001 gtattcttgc tgcgctactt tataaacgtt ttttatctgg agcttggccg attgcacttg
     9061 tctctacaga taatttttca caaaacggta gtcaattacg tacttctttg ctaacgatcg
     9121 caaaagaatg gcaaaaggaa ggttatgtac aaaatacatt tatccaatac ttatcgaatg
     9181 aagaaattgt tgcgtttcca tggacgatga ttgatcgtat caccccgaat ccttccttgt
     9241 cagttagcaa aatgttggca aaagagggct tttcaaatat gtcgattttc caaaccgcaa
     9301 aaggaaataa tattgctcca tttgtcaata cagaggacat ccattattta atcatggaag
     9361 atcattttcc caatggacgt ccgaaattcg aaaaagctgg ggtattcttg actgatcgtg
     9421 caacggtaga taaagcagat acgatgaagg tgacgacttg ccttaatcct ttgcatacgt
     9481 ctttagctat tatgggttgt ttactggggt ataggagcat agctgaggag atgaaagatc
     9541 cagatttatc gctgctagtt tctgaaatcg ggtacaatga aggcctccca gttgtggagt
     9601 ctccaggtat cattgatcca aagatctttc tggaagaagt aattactgtg cgcttttcta
     9661 atccaaatat tcctgatatg cctcagcgaa tcgccacgga tacctctcaa aaggtggcga
     9721 ttcgttatgg tgagacgatt aaaaaatata tggaaataga agttaaagac ccgcagacac
     9781 ttgtattcat accattgact ctcgctgctt ggcttcgata ccttattggc attgatgatc
     9841 aaggaaaacc attcatcccg agtccagacc cactattgac agactttcag cataagcttt
     9901 ccgatattca actgggtacg caagatacat cacatattca tgagaaaatg gctcctattt
     9961 tgtcaaataa agaaattttt ggtgtagatc tttaccaagc agagcttggc agaaaaatag
    10021 aagaaatgtt catcaagatg gtagcaggac cgaatgccgt acgaaaaaca ttacaggatt
    10081 acttacaaga acatggagga aatttatcat ggaaatgact tttagatggt acggatcaca
    10141 ggaagaaaag attaccttag atgaaatcag acagattcct gcggtcaaag ggattgtagg
    10201 cacattgatg gatataccag taggagaggt atggcccagg gagaggatca aagaactaaa
    10261 agaagaaatt gagtctcgtg ggttaacact aaaagtaatt gaaagcgtca atgtgcatga
    10321 tgccattaaa attggattac cttcccgtga gcagtatatc gaaaactata agcagacaat
    10381 ccgaaacttg gcagaatatg gaatagaagt catctgctat aattttatgc ctgtatttga
    10441 ctgggtgaaa tctgaccttg actaccgctt agaagacggc tcatccacgt tagcttttat
    10501 atctgatgat attccagatg atccaagaga aatcgtcgcg cgcatcgaac attccagcaa
    10561 tgaatttgaa ttgccgggat gggaacctga gcgcttagct catatcaaat ccttgcttga
    10621 agcctatgca tcagtagacg aagaaaaact tcgagaaaat tttgcctatt ttctccaatc
    10681 aatcatccca acatgtgaag aggtaggggt caaaatggct gtacatccag atgatccgcc
    10741 atactcgatt tttggattac ctcgaatcgt aaagaatcga gaagatttag attggttgtg
    10801 ccgcatagta gacagccctt ataacggaat cactctttgt acagggagta ttgcagaaga
    10861 tccagaaaat gatgtatatg ggattttagc agaattttgt caaagagatc gaatcccatt
    10921 tgcacatgtt cgaaatatta aatttttagc taatcgagat ttctatgagg caccgcataa
    10981 atccgaatac ggttcattag acatgtaccg catactaaaa acaatgtatg acaacggatt
    11041 taacgaatac atccgtcctg atcatggaag aatgatctgg aatgagacag gaagaccagg
    11101 atacggctta tatgatcgag cattaggtgc tgtttatcta aatggattat gggaagcaat
    11161 cgaaaaagga aacaaaagta aagataatta gtgaaaaaaa ctgtaatttt attatgtaaa
    11221 taagaaggat gttcaaattc ttgatacata aatccggagg ttgttaataa gcattcggat
    11281 ttatgtatct tatttggtct aggaagtaac ttgattttct tttttcgatt gatccatgag
    11341 tgcttcttct tttaattcaa agatatattc tgagatcctc ataatcactt ttttccagtc
    11401 ttctggtgtg atgattcctt ttaaaatata gatttttttg atatgagaag gcaattgttt
    11461 caaagggcag tcgctgataa cgatgttgca gttctccata aaatcatttt gtattttgat
    11521 cgattcattt gaaaaaattt gttctatctt aacgatcaga tctttactga tataaaaatc
    11581 acgcgtatag ttcacaccaa tcacaattcg cggctgtttt ttgtagcgat catacaaatg
    11641 tactaggtct tcaaccatcc attgcacatt ttccttttct aaaataactg gtaacaagtg
    11701 ttgattttgt tctctgaaat ccatataaaa ttgttcaatg gcggtttgga cttctttcaa
    11761 cacgctatgt tcttgataaa gcatgaaagt aggaaggggc gtatttggtt gattgaagtt
    11821 aagaaatcca ttataaagtt tatttcgtgc gaatgccaat gagtaaagaa tcttttcttt
    11881 ttcaggtata aacaaatcaa atacagtaca aaaggactcc accatttctc ttgaataaag
    11941 aacatgagga acgtttgctt tttttaaaag tttgtattgc tccaagcttg tttcttccga
    12001 ttcgattttc gtgatagaaa gtcgagctaa aatattcact aagatgcgtt gttcgcgaca
    12061 aattggcaga ttaggtcgca aaatatctac agagggatca ataaaaaagt caatata
//
LOCUS       DAZUMK010000081        11944 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000081 DAZUMK010000000
VERSION     DAZUMK010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.083"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            16..258
                     /gene="yaaA"
                     /locus_tag="ACG808_002009"
     CDS             16..258
                     /gene="yaaA"
                     /locus_tag="ACG808_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFS5047623.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            245..1369
                     /gene="recF"
                     /locus_tag="ACG808_002010"
     CDS             245..1369
                     /gene="recF"
                     /locus_tag="ACG808_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFS5047624.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1366..3312
                     /gene="gyrB"
                     /locus_tag="ACG808_002011"
     CDS             1366..3312
                     /gene="gyrB"
                     /locus_tag="ACG808_002011"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFS5047625.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3332..5803
                     /gene="gyrA"
                     /locus_tag="ACG808_002012"
     CDS             3332..5803
                     /gene="gyrA"
                     /locus_tag="ACG808_002012"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFS5047626.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6039..6338
                     /gene="rpsF"
                     /locus_tag="ACG808_002013"
     CDS             6039..6338
                     /gene="rpsF"
                     /locus_tag="ACG808_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFS5047627.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6388..6900
                     /gene="ssb"
                     /locus_tag="ACG808_002014"
     CDS             6388..6900
                     /gene="ssb"
                     /locus_tag="ACG808_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFS5047628.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6926..7162
                     /gene="rpsR"
                     /locus_tag="ACG808_002015"
     CDS             6926..7162
                     /gene="rpsR"
                     /locus_tag="ACG808_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFS5047629.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7305..9278
                     /locus_tag="ACG808_002016"
     CDS             7305..9278
                     /locus_tag="ACG808_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFS5047630.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9284..9736
                     /gene="rplI"
                     /locus_tag="ACG808_002017"
     CDS             9284..9736
                     /gene="rplI"
                     /locus_tag="ACG808_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFS5047631.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10010..11377
                     /gene="dnaB"
                     /locus_tag="ACG808_002018"
     CDS             10010..11377
                     /gene="dnaB"
                     /locus_tag="ACG808_002018"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFS5047632.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
BASE COUNT         3986 a         2176 c         2647 g         3135 t
ORIGIN      
        1 aatgagggat gggcattgaa acaacaagtc attctaaata cggaattcat gacgttgggc
       61 caaatgttga aagaagtcac agttatcggt agtggcggac aagcaaaatg gtatttggca
      121 gaaaacactg tgttagtcga tggcgaacca gaaaatcgtc gtggacgcaa actttatccc
      181 ggcatgatgg ttgaagttcc agaagtagga acttttttta tggtaaaaaa aggaaacgac
      241 gataatgagg ctgaataagc tgtatttaaa aaattatcga aattacgaag agctgaacat
      301 cgaattttcc aagaacctcg tcatcttttt aggagaaaat gcgcaaggaa aaacgaatct
      361 tttggaaagt atctacgtat tagctatgac gcgaagtcat cggacaagta gtgagcagga
      421 attgattggc tggactgacg atcaggcgat gattcaagga gagatcacaa aaggttcatc
      481 cactcttcca ttagaaatct tattatctaa aaaaggccga aaaaccaaga tcaaccatat
      541 cgaacagaaa aagttaagta cttacgtggg acaaatgaat gtcattttat ttgcaccaga
      601 agatctttct ttagtaaaag gcagcccgca gaccaggcgg aagtttttag atatggaatt
      661 aggccagatc aatccaatct atctttatga tcttgtccaa taccaagcaa ttttgaaaca
      721 aagaaaccag tacttgaagc aattgaatga aaaaaaacaa actgatttgc tttatttgga
      781 tattttgtcc gaacaactag cagcgtttgg cagcaaagta ttgaaagcaa gagcacagtt
      841 cgtcaagcga ttggagtttt gggctaattc tttgcatcag caaatcaccc atcaaaaaga
      901 gcagctggaa atcgagtatc tcaccgcagt cgattcttta gaaacacata cgcaagagca
      961 aatccaagaa caatttctcg cacttttgaa ccaaaataaa aagaaagacc tttttcgggg
     1021 aacaacgaca gttggtcctc atcgtgacga tctttccttt tttattaatc agaaaaatgt
     1081 gcagacttat ggatcgcaag gacagcaaag aacaactgca ctaagtgtca aattagcaga
     1141 aatcgatttg attaaagaag agacagggga atatccaatc cttttgttag atgatgtgat
     1201 gagcgagctg gatgataatc gtcaactcca tctgctagaa acaatcgaag gaaaagttca
     1261 aacgttctta acaacaacga cacttgacca tgtaaaaaat aaaatgactg ttgaacctga
     1321 gattttttat gttcaacaag ggaaactaaa aggagaagaa actgaatgac agaagaaaga
     1381 agtttagtag aacgcgctaa agagtatgat gccagtcaga ttcaagtact tgaaggactt
     1441 gaagccgttc gtaaacgacc tggcatgtat atcggttcga ctagttctga gggtctgcat
     1501 caccttgttt gggaaattgt cgataattcg atcgatgaag tattagcagg ttttgcaacg
     1561 aaaatccatg tgattattga aaaagacaac agtatcaccg ttattgacga cggtcggggg
     1621 atacctgtag atatccaggc aaaaactgga cgtcctgcag ttgaaacggt ctttactgtt
     1681 ttacatgctg gaggaaaatt cggcggtggt gggtacaaag tttctggtgg acttcacgga
     1741 gtaggatctt ctgttgttaa cgcgctttct acacagcttg atgtcaaagt atacaaagaa
     1801 ggaaatgtgt actatcaaga atatcgtcgt ggagctgtag tggacgactt aaagattatc
     1861 gaacaaacgg atcgccatgg aacaacggtt cactttacgc cagaccctga aatttttaca
     1921 gagacaactg agtttgattt ttcaaaattg gctacccgga tccgtgaact tgctttcttg
     1981 aaccgcggga tgcgtatatc gattgaagac aaacgagaag aagaaccagt catcaatgaa
     2041 taccattatg acggcgggat taaaagttat gtcgaatact tgaatgcaaa caaaaccgtg
     2101 atttttccag aacctgtata tttggaaggt gaacagcaag atatcgcagt tgaagtctct
     2161 atgcaatata cagacggcta ccattccaat atcatgagtt ttgccaacaa tatccatacg
     2221 tatgaaggcg gaacacatga atcaggattc aagactgctt taactcgtgt gataaatgat
     2281 tatgcccgaa aacaaaagct gatgaaagaa aatgacgaca acttgaccgg tgaagacgtg
     2341 cgagaaggat tgacagctgt tatttctatc aagcatcctg accctcaatt cgaaggacaa
     2401 acaaaaacaa aattgggcaa ttccgaagta cggacagtca ctgatcggtt atttagcgaa
     2461 catttcatga aattcttgct ggaaaaccca agtgtgggcc gtcaaattgt tgaaaaagga
     2521 ttactagctt ccagagctcg tctagcagcg aaacgtgcgc gtgaagtaac gagacgtaaa
     2581 ggtgctttag agattagtaa tctgccagga aaattagcgg actgctctag caacgatcct
     2641 gaaaaatgcg aattgttcat cgtcgaagga gattcggccg gcggatcagc taagcaaggt
     2701 cgtaatcgag agtttcaagc catcttgcct attcgtggga aaatcttgaa tgttgaaaaa
     2761 gcaagcatgg acaagatctt ggcaaatgaa gagatccggt ctttatttac tgctatgggt
     2821 acagggtttg gagaagattt tgatgtatcg aaagctcgct accacaaatt agttatcatg
     2881 actgatgccg atgtcgacgg cgcgcatatc cgtacattgc tgctgactct tttttatcgt
     2941 tacatgcgtc ctattgttga agctggatac gtctatattg ctcagccacc tttatacggg
     3001 gtaaaacaag ggaagaatat tacgtatgta caaccaggaa aaaatgcaga agaagagttg
     3061 aaacaagtcg ttgcttcact tcctgcctca ccaaaaccaa gcgttcagcg ttataaagga
     3121 cttggagaaa tggatgatca tcaactatgg gaaacaacta tggacccaag caatcggatg
     3181 atggcccgcg tcagtgtgga cgatgctatt gcagcagatc agatatttga aatgttgatg
     3241 ggggatcgag tagaaccccg tcgtgcattc atcgaggaaa atgcacacta tgtgaagaac
     3301 ttagatattt aatcatgaag gaggaaaaag aatgagtgaa gaaatcagag aaaacatcca
     3361 tgatgtcaat ctaaccagcg aaatgaaaga ctccttcatc gattatgcga tgagtgttat
     3421 tgtcgcacgt gcacttcctg atgtcagaga tggtctgaaa cccgttcatc gccgtatttt
     3481 atacggcatg aacgaattag gtgtaacacc ggataaagcc cataaaaaat ctgccagaat
     3541 cgtcggagat gtcatgggta aataccatcc tcatggagac tatgcgatct atgagtcaat
     3601 ggtacggatg gcacagccat tcagctaccg ttatatgcta gtggatggtc atggaaactt
     3661 tggttctgtt gacggtgacg gtgccgccgc tatgcgttat accgaagcac ggatgagcaa
     3721 aatcgctaca gaaatgctgc gtgatatcaa taagaacact gttgatttcc aaagcaacta
     3781 tgatgataca gaaaaagaac cagttgtact acctgctcgc ttcccgaatc ttttagtgaa
     3841 cggaacaacg gggatcgccg taggtatggc cacgaatatc ccacctcata acttgtctga
     3901 ggtagtagcg gctatcgatt tgctgatgga taatcctgat gtgacgacca atgaattgat
     3961 ggaagtatta cctggaccag attttccaac aggtggtttg gttatgggaa aatcaggaat
     4021 ccgaagagcg tatgaaacag gtaagggttc tattaccgta cgtgcaaaag ttgagattac
     4081 tgagatgcca aatggtaaag aacggatcat cgtgacagaa ttgccttata tggtcaataa
     4141 agcaaaattg atcgaacgga tttctgaact gcacagagac aagcgtatcg aagggatcac
     4201 agatttgcgg gacgaatctt cccgtgaagg tatgcggatc gtcattgaca tccgtcgtga
     4261 cgccagcgca tctgttatct tgaataactt atataaattg acatccttgc aaaattcatt
     4321 tggattcaat atgttggcga tcgaaaaagg tgtgccgaaa gttttgagct tgaaacaaat
     4381 tcttgaaaac tatatcgaac atcagcgcga agtcatcaca agacggacag tctttgaaaa
     4441 agaaaaagca gaagcaaggg cacacatttt agaaggtttg cgtattgctt tggatcatat
     4501 cgatgaaatc attgcgatta tccgcgggtc taaatcagat gacgaagcaa aagcgacgat
     4561 gatcgaacgg ttcgatttat ccgatcgcca gtctcaagct attttagata tgcgtttacg
     4621 ccgattgacc ggtttggaac gggaaaaaat cgaaaatgaa taccaagaac tcttaaaact
     4681 gattgaagat ttaactgaca ttctggcaag accagaacgt gttacagaaa tcatcaagaa
     4741 tgaattagca gacttgaatc aacgatttgg tgataaacga cgcacagaat tactagtcgg
     4801 tgaggtctta agtctggaag atgaagattt aatcgaagaa gaagaaatcg ttattacatt
     4861 gacaaacaat ggttacatca aacgtttggc aaataatgaa ttcagagctc aacgaagggg
     4921 tggacgtggt gtccaaggaa tgggtatcca caatgacgac ttcgtcaaaa atctggtttc
     4981 ttgttctacc catgatacat tgttattctt tacgaataac ggaaaagtgt atcgggcaaa
     5041 aggatacgaa attccagaat acgggcgcac agccaaagga ataccaatta tcaatttact
     5101 aggtatcgat tcgagtgaaa gcatccaggc aatcattgcg gtgacgggag aagcagaaga
     5161 aggccactat ctcttcttta cgacgcgtca aggtacagta aaacgtactt ctgtcactgc
     5221 ttttgctaac atccgaagta atggtctgat cgcaattggt ttgaaagaag acgatgagct
     5281 cgtcaatgtg ctactgacag atggtcaatc taatatcatt attggaacac ataacggcta
     5341 ctctgtcact ttcaaagaag aagcagtccg cgatatggga cgtacagctt ccggggttag
     5401 aggaatacgc ctgcgtgagg aagattatgt cgttggggca gccttaatga aggaagatca
     5461 agaagtatta gtgatcacag aaaaaggtta cggaaaacgg acaaaagtat cggaatatcc
     5521 agtgaaaggc cgcggaggta aagggatcaa aacggcgaac atcactgaaa aaaatggtcc
     5581 actagctgga ttgaccactg tttctggaga tgaagacatc atgctgatta cagataaagg
     5641 tgtcattatc cgtttcaacg tatcgactgt ttctcaaact ggacgttcaa cattaggcgt
     5701 acgtttgatg aagatggaag ctgatacgaa agttgtcacg atggcagcag tcgaaccaga
     5761 agctgcagaa caaacagttg aagaacaagg atcagaagaa tagccatcaa gactaattgt
     5821 gtaagctagc aagaatgacc gattaaaaaa tctggttatt cctgctggct tttttgatta
     5881 gttgaatttc tcttaaaata ttcgaaactg ttcattctgt attgcttttg accaaaatag
     5941 agtgtataat cagagcttgt gagtagtcta attaattaga taactctcct tgctcttagg
     6001 aacctaagag ccgaaagtcc acagggaggt gaatatcaat ggaaaacacg aaatatgaaa
     6061 tcatgtacat tattcgtcca aacattgatg aagaagcaaa aactgcttta atcgaacgtt
     6121 tcgatgcaat cttgaaagat aacggagcag aagttatcga atcaaaagat tgggaaaaac
     6181 gccgtttcgc ttatgaaatg aacggttacc gtgaaggtat ctatcacatc gttaaagtat
     6241 cttctccatc atcagcaaat gctgtcaatg aatttgaccg tcttgctaaa atcaacgacg
     6301 atatcattcg tcacatgatt gttaaagaag aagcttaatt gttgtttcat gtgaaacaat
     6361 agaaaacgat gagaggagat gactattttg attaataatg ttgtattagt tggacgtctg
     6421 acgaaagatc cagatttgcg ttacacagca agtggtacgg cagtcgcaac atttactttg
     6481 gctgtgaatc gtaattttac aaaccaaagc ggcaatcgcg aggctgattt tatcaactgt
     6541 gtgatttggc gcaaatcagc agaaacttta gcaaactatg ctcgtaaagg aacactttta
     6601 ggtgttactg gacgtatcca aactcgttct tatgataatc agcaaggaca acgtgtatac
     6661 gtgacagaag tagtagctga caacttccaa ttgctggaaa gtcgttctgc atctgaaaat
     6721 cgccaacaaa gcggcggatt ccaaagttct ggtcaatcag ctggcggatt tggcggaaac
     6781 aataactcta atcaaacatc tcaatcatcc aacggtatgc ctgatttcga ccgtgatact
     6841 tctgacccat tcggctcaag ttcaacgatc gacatttcag acgatgattt accattctaa
     6901 acgaatttga taggagggaa atagaatggc acaacaaaga agaggcggac gtaaacgtcg
     6961 taaagtagac tacattgctg ctaaccatat tgaatatatc gattataaag atatcgaatt
     7021 actaaaaaga tttatttcag aacgcggtaa aatcttgcca cgtcgtgtaa ctggtacagg
     7081 cgctaaaaac caacgtaaaa ttactgttgc aatcaaacgc gctcgtatca tgggcttgct
     7141 acctttcgtt agcgaagact aataaatcct aggaattgaa tgactgatcc ttttgatcag
     7201 ctattcaatt cctttttttc tgtaaaaata acctctattc gtttagtcgc tcgaatgacg
     7261 aaagttatgg taaaatcaaa ttagtcatat atgggaggaa aattatggaa aataaaaaca
     7321 atcatttatc cccgaaacct ctcttcctta tcctgtttat attaatcgaa gtattggtag
     7381 tcttaatttt accatttcgt tttgttagcg cttcggtcat tctattgatg aatttatggg
     7441 gtgttcgcta ttggttgaaa atgaaacgtt ggcttatttt aaataaacgt gaaaaaatcc
     7501 agcaggcttc gactgtagct gaaaccaatc tagcttatgt ctcagaagtc atgcctgtag
     7561 gagtgattgc ttatcagcca tcaagccaaa aaatcgaatg gctcaatcca ttagcattga
     7621 aaatcaaaga tcaaagtgga ttaacagagg aagaattgct agatactttt tttactgcaa
     7681 aagaaaaaaa gaaaaaccaa gtctatctag cagataaaac atttcagttt gatatggata
     7741 cagtaaaggg tgttgtttat tttaatgata ttacccaaaa ccagttgtta aaactccatc
     7801 agacgaacag tcagccggtg attggtattt tatctattga taactatgca gatgccattg
     7861 ataaattgga tgacaaagag atttcttatt tgaactcttt cgtcactaca ctagtttctg
     7921 actggatgaa tgaataccat gtctttttca aaagaatcaa cgcagaacgg tttttcttcg
     7981 tagcacagat gatcgatctg aaaaagatga tggataaaaa atttgatttg ctggatcgct
     8041 ttcgagcaga agctgaaaaa cagagctcgc ctatcaccct tagtatgggg attgcttatg
     8101 gctcaagaac cctcgatgaa atcggtggag aagctcagaa taatttagat gttgctttag
     8161 tccgtggagg cgaccaagtt gtcgtgaaag aggtaaaaga aggagcaaaa cctgtttatt
     8221 atggggggaa atctgctaag aatgtcaaac gcacgcgtgt tcgttctcga gcgatgagta
     8281 ccgctttgcg tcggattttt gatgaaacag gcgatgtgtt tatcatgggg cacaagtttc
     8341 cggatatgga tgctttaggc tctgcttttg gtgtcagttg tctggcacgc ttcaaccaga
     8401 aaaaagctta catcgttatc aacgagaacg agatcattcc agatgtagaa cggtgtcttg
     8461 aagaaatcca taagcatcct gaattagaaa tgcaactaat ctctccagaa cgtgccatag
     8521 agctgaaaaa agaagaggac ctcctcgtga tggtggatta ccataaacct tctctatcga
     8581 tctctcaggg cctatatgag caatttgata agattgtgat cattgatcac catcgtcgtg
     8641 gggaagagtt tccaagtaaa cctctgctga cttatatcga atcttcagct tcttcagctt
     8701 cagagctagt ttctgagctg atccaatacc aaagcagcaa aacaaatcgc ttagatcgtt
     8761 ttgaggccac attattgctt gcagggatgg ttgtagatac aaatagtttc aatgtccgta
     8821 caactgcacg aacttttgac gttgccagct atttaaggtc ttgtggagca gacgcatcct
     8881 tagtacaata tttattgagc tcggatttag cgacatactt ggagatcagc aatttgattg
     8941 ctggaagcga ctatgtgact tctgatattg ttgtggcggc agggacagac gaaaaagagt
     9001 ataatagtgt tatagcagca aaaacagcag atacattgct ttcgatgatc aatatcaatg
     9061 cagctttcgt cattacaaag cggacagacg gcttgattgg gatcagtgcc agaagtaccg
     9121 gcacgatcaa cgtccaaatc atcatggaat ccttaggtgg cggaggacac ttcacaaacg
     9181 cagctactca actggaaaat acgacagttg aggaaacaaa acagaagcta tttgctgcaa
     9241 tcaaagaaaa tatgaatcaa atatatgata aggagtaatc caaatgaaag taattttttt
     9301 acaagatgta aaaggaaaag gtaaaaaagg ggacatcaaa gaagttccag caggttatgc
     9361 acaaaattat ctaatcaaaa ataacttggc aaaagaagca acgaaaagca gtatcagtca
     9421 gttagcagga caaaagaaag cacaagaaaa acacgaagct gaaatcttgg aagaagctaa
     9481 aaagttaaaa gaattcttgg aaaaagaaga tactgtagtc gaattgaagg cgaaagcagg
     9541 ggaagacagc cgtttgtttg gttcgatccc ttcaaaacaa attgctgaag ggttaaacaa
     9601 acaatacaaa atcaaattag ataaacgaaa aatcgaatta gaaaatccga tccgttcact
     9661 aggctacaca aacgtacctg ttaagcttca tcatgaggtg acggctacga tcaaagttca
     9721 tgtcgtacaa gaataacaaa tcttgatagc gatgttcagc tggtaagtat aaccagaagg
     9781 aaaaaagaca aaccgaaatt tatctgagaa aacagctgca gcaatgaaag caaaagctgc
     9841 aaatcgattt cagattgatt tcggtttctt ttttgggaac agcaaaatag cataaagaaa
     9901 acgagtataa taaaagagaa ctattttttt attacacttt cggtacgaat gatgactttt
     9961 ctctcatttt cttgtaaaat gaagcaattg aagacaagga gcgatacgta tgaatgaagt
    10021 gtggcaggat cgaatcccac cgcaaaatat tgaagctgaa caagcggttt taggctctgt
    10081 ttttcttgac gcagaaacga tcattgatgc catggagtac atagagccaa aagactttta
    10141 tcgtcgtggc catcaaatta tttttgagac aatgatcgag ttaaatgatc gtaatgaagc
    10201 gattgacgtg gttactgtca aagaccggct ggaacaagcc aatttgttag aagatgccgg
    10261 tggtttaagt tatctctcag acttggcatt agcagttccg acagcagcaa atatcgtcta
    10321 ctatgcgaaa atcgttgaag aaaaatcatt gctgcgtaca ttgatccaga cagcaacagg
    10381 aatcgttacg aaaggattcg agcaaggaga agaagttcaa acaattttag atgatgccga
    10441 acgcagtatc ttggaggttt cggaaaaaag aaatcgaagc ggctttttat ctattgctga
    10501 agtactgaac tccacgattg aaaatatcga tcgactagct caaaacaatg aagaaatcac
    10561 aggtctacca actggctatc aaggtctgga taaaatgaca gcaggtttcc agcccgaaga
    10621 actaatcatt cttgctgcac gtccagcggt aggtaaaaca gcgtttgcat tgaatattgc
    10681 tcaaaatgta ggaacaaaaa cagatcgttc tgttgcgata tttagtttgg aaatgggggc
    10741 agaatctctt gtaaatcgga tgctttgtgc agaaggttcg attgaggcca gccatttacg
    10801 tacaggtcag ctttcggaag aagaatggtc gaatctgatc gttgcgatgg ggagtctttc
    10861 tcgagcaaat atttttatcg atgatacacc agggatcaaa atctctgaaa tccgtgcgaa
    10921 atgtcggaaa ctagcgcaag aaaaaggcaa tctaggcttg atattgatcg attatttgca
    10981 gttgattgaa ggtaacggta aagagaaccg tcaacaagaa gtatcagata tctctcgcca
    11041 actgaaaaaa ctggcgaaag agctgaaagt acctgtcatt gctctttcac agttatctcg
    11101 tggagtagaa cagcgacaag acaaacgacc agtgctaagt gatattcgtg aatccggatc
    11161 aattgaacaa gatgcggaca tcgtagcctt cttgtatcga gatgattatt atgaaagaga
    11221 aggcggagat gacgatgatg gaaacgagcc aaaaaatgac aacgtcatcg aagtcatcat
    11281 cgagaagaac cgaagcggtg cacgaggaac agttgaacta ctgtttatca aagaatacaa
    11341 taagttttcg tcgctttcgc caagagaaga attttaaaga agaggttgtg agccttgagc
    11401 aatcccgaga gctctcgtaa gaagatgcaa ggagtaaccg caggaacgac tagagtactt
    11461 aatttaaaca gatctccaaa gggattgtga caaaagtctt gtcacagtcc ctttttccga
    11521 ataaacggtg ttcaatcagc tgacccttgg tattagctca gcaaacgtca aaatatgagg
    11581 aactattttg acgtttgttt ctctaatact caggtcaaaa cgctgatctt acgacctctt
    11641 ttccttaaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat catcaaacat
    11701 gagaagcagt tttttgaaat tgctccttgt gactcagagt taaacggcag gctcacaacc
    11761 tctttcttaa acggtgttca aaagcttgca tctgcggtaa tacgcccaat caaacgaaaa
    11821 atatgagaag ctatttttgt ttggctgttc ttattacttg atgcaggaca gctttttcac
    11881 aacctctgat tcacggtgtt ccaaaagtag cttcttcgaa aaataagtcg aaatttcaca
    11941 aaaa
//
LOCUS       DAZUMK010000082        11803 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000082 DAZUMK010000000
VERSION     DAZUMK010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.084"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..195)
                     /locus_tag="ACG808_002019"
     CDS             complement(<1..195)
                     /locus_tag="ACG808_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047633.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNY"
     gene            complement(211..825)
                     /locus_tag="ACG808_002020"
     CDS             complement(211..825)
                     /locus_tag="ACG808_002020"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFS5047634.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(972..1655)
                     /locus_tag="ACG808_002021"
     CDS             complement(972..1655)
                     /locus_tag="ACG808_002021"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFS5047635.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1764..2222)
                     /locus_tag="ACG808_002022"
     CDS             complement(1764..2222)
                     /locus_tag="ACG808_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFS5047636.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2306..4069)
                     /locus_tag="ACG808_002023"
     CDS             complement(2306..4069)
                     /locus_tag="ACG808_002023"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFS5047637.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4290..5750)
                     /locus_tag="ACG808_002024"
     CDS             complement(4290..5750)
                     /locus_tag="ACG808_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047638.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5958..6884)
                     /gene="glsA"
                     /locus_tag="ACG808_002025"
     CDS             complement(5958..6884)
                     /gene="glsA"
                     /locus_tag="ACG808_002025"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFS5047639.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6907..8334)
                     /locus_tag="ACG808_002026"
     CDS             complement(6907..8334)
                     /locus_tag="ACG808_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS5047640.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8365..9579)
                     /locus_tag="ACG808_002027"
     CDS             complement(8365..9579)
                     /locus_tag="ACG808_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFS5047641.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(9665..9892)
                     /locus_tag="ACG808_002028"
     CDS             complement(9665..9892)
                     /locus_tag="ACG808_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047642.1"
                     /translation="MKNKFENRSDTRCGSFLYILYEVFFIPYEKISLKIKKDENKRFS
                     IKRRPFIFCLYLCEKRKRAQHIKLLLIVCLL"
     gene            complement(9920..10162)
                     /locus_tag="ACG808_002029"
     CDS             complement(9920..10162)
                     /locus_tag="ACG808_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS5047643.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(10194..10751)
                     /locus_tag="ACG808_002030"
     CDS             complement(10194..10751)
                     /locus_tag="ACG808_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS5047644.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(10764..10952)
                     /locus_tag="ACG808_002031"
     CDS             complement(10764..10952)
                     /locus_tag="ACG808_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFS5047645.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(10965..11531)
                     /gene="amaP"
                     /locus_tag="ACG808_002032"
     CDS             complement(10965..11531)
                     /gene="amaP"
                     /locus_tag="ACG808_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFS5047646.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         3461 a         2447 c         1933 g         3962 t
ORIGIN      
        1 gtaattaaac aatccgctaa tcgtttgatc tggcatttct atttccttac catctaaagt
       61 aacagtagta acgacaaaat ggtcttgcaa tggcggaact tctaagtact gcccttgcag
      121 aaaatcattc actgtttcat attctttttt catttctttt gaatctgtcg atggtttata
      181 gcttattttt aacataacta gtgctccttt ttattcggga cgtgcaatcg tgaaaatttc
      241 tcctattgct gcataatcat ttcctgccaa acggcttaca gcattcaatt ttcttggatc
      301 gatccttcct gtcttatcat catagactgt ttcatttaaa tgatatttct gtacttctag
      361 caaaaataaa tcagctgtta ctttattttt attccgtatc tcgacatgtt catataagga
      421 tgtttcaaaa cgaacttttg cttctgtcaa tgctggtact gaaatgatta ctgaatcagt
      481 taaagtcaag tcagtcaggt acagttcgct ttcctcatta ggcagattgg ctgctgtttt
      541 atttacttct tctacgttgt cctcatcaag gatatggata acagcttctc ttgtctcaag
      601 aatattcttt gctgtgtctt ttactgcatc ttgttttctc tggatcgcca aggataagat
      661 gggtcgattc gaactgacga tattgaagta actgaagggt gcgatattca ctatatcttt
      721 tgctgattta gtcgttacta ctgctactgg tctagggatg actgaaccaa tcagcaactt
      781 gtaattttca cgttctgtca attctgaagg actccgttca aacatggttt cgccttcttt
      841 ctataaaatt cgttaaaaag cagatcttga agacaaaatg aaaagtaatg cttttttcat
      901 cttcttagaa tctgctttaa actatctatc aatttttctt ttttgtcaag aatctgtgcc
      961 gtgcgtgttt tttagttatt tgctttactt gcggcttcga catatctttt agtgatcaat
     1021 tgcggacggg tgataataga tccaactacg actgaaaaag cacctaattc atatgctttc
     1081 tctacttgtt caggagagtc agtatgtcct tccataatta ctgggatatc gacttgttct
     1141 atgatttgat tcaataaatc aaaatcaggt gtctgcgtct ttcgtgaata ttctgtatag
     1201 cctgctaaag tcgtagacaa aagatcaaaa ccatcttctt gcgcttgaag cgcttcctca
     1261 aaagtcgaaa tatctgccat agctaaccga ccgtgttcgt gaatcatttg gacaagttca
     1321 gcagttgttt tttctgtcgg acgtttacga gaagttgcat ccagtgcaat gatttctgct
     1381 tgcgtctcca aaagttcttc cacttctttt tctgtcgcag taataaacac ttctgaccca
     1441 tcgtaatctc ttttgataat ccctattacc ggtaaatcaa cgattcgctt gatttcattg
     1501 atatcttctg tcgtatttgc tcgaatacca actgctccgc cttcttttgc agccaaagcc
     1561 attctcccca taataaaaga actgtgtagc ggctcgtttt ccaaagcttg acaagaaacg
     1621 acgactccgc ctttgatttg ttccagtgta tccacaatat ctcctccagt aatgttatat
     1681 aaaaatacag cttcatataa aaatgtaacc cttttctatt caggagacaa taatgagcta
     1741 tttctttcag cagacctttc ttactaaaag aaaattactt caaattcttc gtatacgacc
     1801 agcttctctt ctgtaaattc tatgttcaac gcttctttat aagcatgttt aaaaaactca
     1861 gcatggactt tccgccaata agctaatgtc cgatcccctt ctccttcttt ccaagcatgc
     1921 tctttcgtga cttcactaaa aggcaagatg gtcacattta tatttttgat gatacaaact
     1981 ggttcatttt ttccattcag gaccacgtct aattgaccaa cttttggtaa aggctcattc
     2041 tcttgtttat acagatcgta accacttgat gtaccagtct ttatcttttt tacaactagt
     2101 tcgctcaact cgtctgccat tttttctgta tccccaaatg aaaagaaacc taatttttct
     2161 gattgataac taggatattt ttcttggaac tttttgacat attcttctac ttgctgatac
     2221 ataacatcca cccctttgct ttttataatg aaaaaaccat ccagatacta gtataaacaa
     2281 ttatctggat ggtgagcata atgaattatt gaatattcaa gacacgcaga ctatcttcaa
     2341 cgatttgttt tggtgttaaa tgataacgtt cataaagctg ccctacagga acgctatccg
     2401 tgaattcttt ctctgcacca aaatttaata cgttcattga agaatcacca taataacgac
     2461 tgatattttc gccaaatcct cctgacaaac taccatcttc taatgtgatg actaattcgt
     2521 gtgtttcttt taatttatct aatgtttcta cgtcattttc tgtgatcgtt cctggattga
     2581 tcaacgttgc ttcgattcct ttttttctaa aagcttttac tgtttctttt cctaattcaa
     2641 gaaaaccgcc taaagctaaa attgctactt ttcctccatt atttactgtt tgataggctg
     2701 gaatactata atcatttgcg atagctgtac catgttctac accatgtgta ggaattcgga
     2761 ttgctacagg ctgcgctgtt tgttcgaacg cccaatctag catagagatc atatcctctt
     2821 ttgttgctgg agcaagatat ttgatgttcg ggatacttga taagtaagga atgtcaaatg
     2881 ttccttggtg tgttcggtca ctagctgaga tcgttccgcc accgactaaa ataatcactg
     2941 gattctcgtt gatcgctaaa tcatgtgata attgatcata tgcccgttgc aagaaagtac
     3001 ttgattggaa gacgactgga cgtactccct gagaagccaa agcagaagcg aaagtgatag
     3061 agtgctgttc agcgatcccc acgtcaaaat aatgatctgg atgtttttct tggaatgttt
     3121 tcagtccaaa ggcgcctggt atcgctgcat tgactgcggc aataggagct gtacttgttt
     3181 gcattttctt atccaaataa tccatgatga tcatattaaa tgtttctcca gaagttactt
     3241 gctttgcctc cccggtggac agatcaaatg gtacatgcca atgatatttc attttattat
     3301 cgattgccgg ctgataaccg cgaccttttt ccgtatggac gtgaagtaca atcggatgat
     3361 caatatcttt tacttcttca aataaacgga tcaccgtttc cagatcgttt ccattatcca
     3421 cgtaacgata atcaaatccc attgctttaa ataggttatt gctgctttta ccgttggttt
     3481 gacgaagctc agctaagttt ttatataaac tgccatgatt ctcatcaata gacatctcat
     3541 tatcgtttac taatacgatt agattggatt gtaaaacacc accattgttc aatccttcaa
     3601 aagctaagcc accacttagg gagccgtcac cgataaatgc taccacattt cctgattctt
     3661 tcttcagatc tctcgcttta gctaaacctg ttgctaagct gatagatgta gatgtgtgcc
     3721 ccactgtaaa atagtcatgt ttactttcat gttggttgga atatccgctc acttcacggt
     3781 aatgtccttt ttcaaaacca ttttttcgac cagttaaaag cttatgacca taagactgat
     3841 gggaaacatc ccaaacgatt ttatcctttg gtgaatcaaa tacataatga aacgcaatcg
     3901 ttaattctac tacacctaga ttcggaccaa cgtgcccacc tacatcgctg tccttttgca
     3961 agattttttg gcgcatctct tctgccaact gtttcatttc ctcaacagat aattttttga
     4021 catctgcggg gctattgatt gcatccaata catgtaatac tgtactcata atacattctc
     4081 tcctccgtac tatcttcttt taatgaaaat agatttgtta cactttatct attctactac
     4141 ttagagttga ctctaagtca atagcatttt aaaaaaattt cagagaaagc ttgttatata
     4201 agtagatctt cgcacgaatc aggactcaaa taaaaacctt ctacagacaa ttaaaaagct
     4261 ttgtagaagg tctcctaatt aaggtctcat caagaaatat gtttttttaa taggtgaagt
     4321 tcctgagcaa tatgatacaa atggatgttt gcacctttta atgagattgg atagatcttt
     4381 gtcttttcag tttctaatcc attttgatag ctagcagcat cgcacaaaac cactcttttt
     4441 ttcgcacata gacattcatc aggcaattct tctaatgaac gaaataaaat aggatttatt
     4501 tcagcgatgc cttcttccgc atacaaatga aattgggttc gataaaaaaa ttgtttgact
     4561 gaactttctg aaacaacaaa aatttctatt tcctgtctcc ccgcttgttc actgttgatc
     4621 aaagaaagac ttttgacgaa ttttcgaatg aaattttcat tccatcttag ttggatttga
     4681 tttttctttt tccactcttc caatatttct acaatttttc cgtacagaag ttgttctttt
     4741 tctgatagaa gatagatcct ttccggtaaa aatacttgtc catctgccca caacgtacgg
     4801 acaaattgta aaaaggattg cttgaaaata aaattttctg catcgtcgat ttcagcgata
     4861 ctaataatct gtaaaatcaa ttcattcagt gataggtcat tgagaacaaa atttttataa
     4921 acaacttcga aatcttttct taacagctcc atgttctcat tggtataatt tctactgttg
     4981 aacaaagaaa aaatataata gacatcttct tcttgtaagt ctagaattcc tttttgctct
     5041 tccaatcctt gacagactaa ttgaaataac ggcttcgttt ctgcgctttt cttatcttct
     5101 tctttcagtc caaggggaaa tgcctttctt ccgattgcga tctctattcc tcgtatgata
     5161 aaggactttt catcttccga atatcttctt ttaaaaaaat gagtctctac ataatcaatc
     5221 aactcgttac aagcttcttc tacatacgga tttttatttt catagccttc ccaattgaca
     5281 taacgtacta aagctagcaa gatgttccgc gagtcaaatt ctgggataat gatttcttta
     5341 tttttcaaat aatcattttc tttaaaaaaa tccaatacga tttttttgat tgtatatact
     5401 ttgctcaaag aaataaattc aagatcagcc agttctgtac tcgtgaatat accattaaaa
     5461 taataactta aaaactttaa aaaataagat tgaccgtaca gaaactgtgt gagctcagaa
     5521 agagcaatac catgtttgaa tacgagttta tacttatgct tctctcttac ttgttctttt
     5581 atacaatatt ctagacactc ggctagactt tctagatcgt gaaggatcgt tggtgtagtc
     5641 acccttagag aaatagctgt ttgttcgatg tctatttctt ggaattcaaa taattgttca
     5701 cacagataca cttttctaaa tatatttcgc tcgatgtaat tttccagcat tcctgataca
     5761 cgcctccagt ttttcttatg attttattta atcatatctg taaatcgtat ttttgtttcc
     5821 tttttattta gaaaagccct ttcaaaaacc aataaaaaaa gaaactggga caaaagtcaa
     5881 acaagctcac aatttcagcc tcattgtagg tagctttgag ccgtttggac ttttacccca
     5941 gcctctttca agaatggtta ttcaaagatg tcagcgtcta gttctttgac tacatctttc
     6001 aacaattgga taccagctgc gctgttacca aatggatcta gtgccgggct gaagacacct
     6061 atgccgtaac gatttggcac agctgccatc aacccgccgc ctacaccgct ttttgcaggt
     6121 acaccaacat ggacggaaaa ctctcctgat tcatcataca aaccagcagt tgtcataatt
     6181 gatttgacga ttgtagcact ttcttcagta atgatttgtt tgccattcca aggagcaatt
     6241 cctttatttg ccagcacagc agctaatttt gctatgcctg tagcggtgac attgagggag
     6301 cattgtctga aataagcatc gagtatctcc ggtacgtctc cttcgatcat ttgattacct
     6361 ttcatatagt aagcaagaga gcgattgata tcacctgttc ttgattcaga atgatagatt
     6421 tcttcattca atgtgatatt aggatcatca cagatctctt tcataaatga caagattcgc
     6481 tcaaattttt catctgcatc tttaccggca atcaacgagg ttgttgcaat cgctcctgca
     6541 ttaacaaaag gattcattgg atgtttgcga tgattgattt ccatattcat gatagaattg
     6601 aaggcaaaac cggttggttc cgtgttgatt gtttgaaaga ctttttctat tccgttgtct
     6661 ttgattgcta ataacaatac gggaactttt gacatgcttt cgatggcaaa gcgcacatcg
     6721 gcgtctcctc cctcaatcag ttgttttgta tccaaatcat aaatcgccat acctaattgt
     6781 ttgggatcca cttcagctaa tgctggaatg taatccgcca cttgtcctgt ttgataatag
     6841 tggaaatttt ttccaatcaa ttgttctaat tcttttactt ccatttgaat aaccagcttt
     6901 cattttttat ttttctgcat gctggaattt atgacggcat gcgtagatcg taaatggaat
     6961 aactaagacg ataagataag aaacaatcag aatcgtttca taaatcccat gctgtccagc
     7021 tgcgatacta gcaggtggaa caaatgagat acaaaaagca aaaagtgaag tcaaaagacc
     7081 taaacaagca acgattactt ttacaacttt tccgcctgga acctgatagg ctctttcaag
     7141 actgtcatct ttaaaaacca gcttcaaata gcccgcgaaa aacataaagt acaacatcag
     7201 gtagattaca acagtcaatg acatcgctgc aagaaatgaa agattattcc ccccgccgct
     7261 taatgtaaag acggctgccc agattgttgc aacgatccca tttgcgatca tcaaatgagc
     7321 aggaacaccg tgtgtgtttt cttttgcaaa atattcagga agtaatcctt cttctgctgc
     7381 tacgtgaagt gcttttgaag gagaggttac ccaagaagca atttctccga tgacacctaa
     7441 acaaacaaga atcgcaagaa ttttcgcaat ccatgcaaga tttggatcaa tgtgtcttaa
     7501 taggaattct aacgattgaa tcacccctgt attaagagac aaaccatgtt gaggtactgt
     7561 agtagcgacg gtcaatccgc caaaagtatc taaaatgatt gccagaataa ccaatacaaa
     7621 cattgctaat ggataatcct ttttagggtt ttccatttca tttgcatgac tggcagatgt
     7681 ttctacgccc atatatgcta aaataaagga aacaaaaaca accagcgtag gcaatttcga
     7741 aaaatcagga atcaatgttt tcatagacag tatcgtttgt agtggagcgc ctgaagcgac
     7801 ataatggatc cctaagccaa acaagactaa tgctggcaac aagataccag ctacaaaacc
     7861 gatccgagca atcaatgaag tgtatttggt tccttttaac tgtgagagta aaatagccca
     7921 gaaaataatc aataggattg ccaatttcag atatttattg ttttgtatag caccaatacc
     7981 cgttgcatag gataatgcac cagttaagaa atacagcatg gtcaagtagc caactgttat
     8041 ctcaaaccat tggaaaaaga ttgcggcaaa accgaatcgt tttcccaatg tgcgactgac
     8101 ccaagtatag acaccgcctt tttcccatcc tttcaccgtt gccatctctg ctgcgcacaa
     8161 tgccacaggg ataaaccata gcaaaccgcc taataacaag aaaaatacta agctgaagcc
     8221 tgaggtcgcg aatgtgggat attcatataa tgaaacgacc attgagatgg tcatagccaa
     8281 aaagccaaag aacgttaatt tttttgcagg aattttcgtt ttgctttgtt gcatgaatct
     8341 tcctcttttc tatttgaatt tgtttcaatt gagtttgttg ccaaagaaga aaaagtccaa
     8401 tccttcttct tgttcttctt ttgtagtagt gatcgttgta aagtgcatcg ccgattttaa
     8461 aatcactgtt gtcagcacat aggcatacac ggcagtaatg atgacaccaa tcacttgaat
     8521 ccctaagaag taccagctgc ttggattcgt tagtaaatgg ctgttagatg caaagatccc
     8581 tatcagaatc gtacctgtga atccgcccat gccatgaaca ccccacacat caagtgcatc
     8641 atcccattgt ttctttttac gaaaatcaac agcagcatga cagacgatac ccgcaatgat
     8701 cccaatcagc atggcacttt ttgcttctac ataaccggca caaggcgtaa tggctgccaa
     8761 tccagcaacg gatcctgtta gtacgtcgac aaaatcaaag ccatttcctt ttacttttgc
     8821 aatgatcagc caagtaatca tcgcaaatgc aagagcgatg aatgttgaac caaatgcatt
     8881 gactgcttgt tcatctgctc gaagtgcacc accggaatta aaaccgaacc agccaaacca
     8941 aagcagtcct gtaccgatag cagctgccat aagactgcta tgctgacttc tttctcctaa
     9001 aggaatcttt ctttgaccaa gtgtgaggat acatgctaaa gagccgaatc cagctgtcgt
     9061 gtggatgact gctccaccag cgaaatctct aaatcccatc tgatccaaaa atccgccacc
     9121 ccatatccag tggcaaacag ggaaataaat cagcagattc caaaaaataa caagtataat
     9181 atagcctttt aaattcaatc tccctgcaaa agctcctgtc atcaaaggaa cggtaatcac
     9241 gataaacatc agttggtaaa gaaagaacat taaaaatgga atcgttgcac catgaaacat
     9301 gttggggaag cttgtaacat gtctcatcaa aaagaaatct gccgggtttc caataacacc
     9361 gccgatatcc cgtccaaagg ccaatccgaa tccaccaaat acccacataa tcgttgttat
     9421 cccaacggag agaaaagatt gagacatgat cgttagtata ttattcttcc gtgctaatcc
     9481 gccgtaaaaa aaagctagac caggagtcat caggcaaacc attgctgtac ataaaatcat
     9541 gaatgctgta tctcctgtat tgataggtaa tgtattcatt tttttcgcct cctttctacg
     9601 attacgagta taaaagaagg tgtagttgtt tttttcttct gtttttttga attaatactt
     9661 gcatttataa aagacagaca attaataaaa gcttgatgtg ttgtgctctt tttcgttttt
     9721 cacacaaata aagacaaaaa ataaacggtc ttctttttat agaaaaccgt ttattttcgt
     9781 ctttttttat ttttaaagat attttttcat aaggaataaa gaatacttca tataaaatat
     9841 ataaaaaaga accgcagcga gtgtcgctac ggttctcaaa tttgttcttc atttcaacat
     9901 aagtttttct tcaatttgct tataatttac gtgcaaagaa tgaaacgact aatactaaaa
     9961 tgattgcacc gatcaatgat ggaatgatag ccattcctgc aattgttggt ccccaagttc
    10021 ctagtaaagc ttgtccaatt gctgaaccta ctaaaccagc aatgatgttt gcgatgatcc
    10081 ccattgatga tcctttattc gtgattgctc ctgcaattgc tccaatgata ccgccgacga
    10141 ttaatgccca aataaatccc attttaaatt cctcctaact gacttcttac ttcttattgt
    10201 acacgtggtt cgttattttc tttgactttt tgtgcacctt tgcctacagc agattttgct
    10261 ttatctgttt ggcgtgaagc aaattgtcct gtggattttg ctgcttctgt tactttgtct
    10321 tgtactgttt cttgatcttt ttggtattct tcttgtgttt tgatgtctgc aacattcaca
    10381 ttcacttcga taacatctaa atgtgtcata ttgtttactt cagtactgat caattctttg
    10441 atttggttgt aaatatcttc gatgtcttta ccgtattcta cgatcacatc tagatccaca
    10501 gctacttgtt tcttacctac ttctgtattg atacctgctg ttacgttatc tgtgttgact
    10561 aatttttcag ccatatttga gaaaaatccg ccatctaatg tcaatagacc atctacatct
    10621 ttcaatgcat aaccgatgat tttttggatg actttgtctt cataagtcaa ttcaccattc
    10681 atcatgcggt tttctccttt ttcatttgcc aaaactgatc catttgttgt attcatgttt
    10741 ttgttttcca tctttgtttc ctcctatgat ttaaaaaatt gatcaactat tcctgactgt
    10801 ttcaaataaa aaccgattcc tgcgccaatt gctgtcatga ttatgatcaa caatgtcttg
    10861 aaaaatccaa tcgttaacca taaaacagct agcagtagtc caagaccccc aaagataata
    10921 gggagcttgt atgctgaaaa tagttctttc attatttggc cctcctattc cactcgagag
    10981 tgcttgtagt ctcttgtatc ttcttgttct tcataaccgc tgtatgtgac tgccactttg
    11041 accgtttctt ttgttccgag tactttagtt acttcttctt cgatatcttg catcaataca
    11101 cctgttttcc ctactaatga agaagttcgt ttcaagtctc cgttgacatg gatatggatt
    11161 cgatttttcg ttgaacgtat ccgtacttta ggtgaatgaa tgaactcttc ttcgtgtaaa
    11221 tgggaacgta ccaacccttc gatcgctttt ttgtcgattg ttagtttgcc atcttcccgt
    11281 ttcataacga aagttcctct tgactttgga tagaaaagaa cgactagcaa gatgataata
    11341 gcaagaacgg caaatactac agctgcccag aaaatatatt gttgcatgta gtaattcgtt
    11401 aaagtaaaga agcgaaatcg ttccaattgt tcaggcataa tatacggcgc attgatcaac
    11461 gcaatcgcta gaaatactga aagtagcagt aaactcatga taacaccgat tatttttaac
    11521 cccttattca ttttccacct cctataaact tatttttacc ctgtaaataa ctctaccgac
    11581 aatcacagta tagatagcga acagttagca tagcatcagt aaataaagcg ctaccaaaca
    11641 ttgctggatc ataacggcgt tttattgcca gtctgatact gaaataacaa ttgcttactt
    11701 attctctatt tacttttgat tgctggctat acttaaagga tattattcaa gattaaaaat
    11761 tgcaaagatt atgctcagtt attattacaa gattctattt att
//
LOCUS       DAZUMK010000083        11747 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000083 DAZUMK010000000
VERSION     DAZUMK010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.085"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            23..763
                     /locus_tag="ACG808_002033"
     CDS             23..763
                     /locus_tag="ACG808_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFS5047647.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGETNYIESSKN"
     gene            complement(855..2042)
                     /locus_tag="ACG808_002034"
     CDS             complement(855..2042)
                     /locus_tag="ACG808_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFS5047648.1"
                     /translation="MYFLGIDIGKRTHVASIMNEEGKVLLKGFSFPNTTEGAESLIER
                     MVDYSGAPSDFVIGMEATGHYWLSIFSYLHESDYLIHVVNPLQTDGWRKGTEIRKRKN
                     DIIDSVLIADLMRYGSFVETVLSDENVFSLKQLSRYRTYLVGTASDFKRKIIAVLDQV
                     FPEYATIFTKQGVFGKASKELLLAFSAPIDLENISSETLAQYLAELSRNRIKAEKADQ
                     LKQAASNSFGVKFAQEAFTFQLRSMIEQLKFIESQIKETEREIKHIMDTLNSVILTIP
                     GIGPINGATILGEIGDIQKFSNPKKLVAYAGIDASVTQSGQYEATHNVMSKRGSPYLR
                     KALFSAALVASQCDPVFSAFYQKKVSEGKHHLTALGAVSRKLCYVIHAILTKNEPYII
                     VSD"
     gene            2364..3206
                     /locus_tag="ACG808_002035"
     CDS             2364..3206
                     /locus_tag="ACG808_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFS5047649.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            3203..4153
                     /locus_tag="ACG808_002036"
     CDS             3203..4153
                     /locus_tag="ACG808_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFS5047650.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            4168..4401
                     /locus_tag="ACG808_002037"
     CDS             4168..4401
                     /locus_tag="ACG808_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFS5047651.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            4732..5316
                     /locus_tag="ACG808_002038"
     CDS             4732..5316
                     /locus_tag="ACG808_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5047652.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            5447..6115
                     /locus_tag="ACG808_002039"
     CDS             5447..6115
                     /locus_tag="ACG808_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFS5047653.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            6126..7541
                     /locus_tag="ACG808_002040"
     CDS             6126..7541
                     /locus_tag="ACG808_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5047654.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQNPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            7559..8128
                     /locus_tag="ACG808_002041"
     CDS             7559..8128
                     /locus_tag="ACG808_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFS5047655.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVVSWVLMQLGSAAGQWIPIWFFTDRFRQDWIDRGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            8238..9992
                     /locus_tag="ACG808_002042"
     CDS             8238..9992
                     /locus_tag="ACG808_002042"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5047656.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQYLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHRVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            9952..11718
                     /locus_tag="ACG808_002043"
     CDS             9952..11718
                     /locus_tag="ACG808_002043"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5047657.1"
                     /translation="MQMIGGLTMKNFYKKKFALTDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENAKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
BASE COUNT         4054 a         1640 c         2173 g         3880 t
ORIGIN      
        1 tatcatttag aaggagaaag ttatgttaga agtgaaaaag cttaatgttt gttataacga
       61 ttttttagca ttgaacgaga tttctttaaa aatacaagaa ggtgctttaa caggtattat
      121 tggtccaaat ggtgctggaa aatctacatt gcttaaagca atgttaaata ttatccctca
      181 tcagggagag gtctccatta ataatgaaga tatcaataaa aaactggtga agattgcata
      241 tgtagagcaa aaagctgata ttgattttac ttttccaata aaagtaaaag aatgtgtatc
      301 tatgggaaca tatgccggaa tgaaagtttt tcaaagaatt aaaaatgcag aatggcaaag
      361 agtttcaaag gctttggaaa aggtcgatat gggaaaatat tctaatcatc aaataggaga
      421 attatctgga ggacaatttc aacgtgtact tttagcaaga tgtcttgctc aaaatgcgga
      481 tttcatattt ttagatgaac catttgtagg gatagactta gttagtgaac gaataattat
      541 ggatacgtta aaagaattga aacatcaggg aaaaacagtg ctaattgtgc atcatgattt
      601 aagcaaggta aagaagtatt ttgataatat tattcttttg aatcgaaatc taattgctca
      661 cggaagtgta gaatctgtat ttaatgaaga gaatttaaaa aaagcctatg gagatactat
      721 atttattgga gagactaact atatagagtc aagtaaaaat tagccaaatc aaaaaatatt
      781 ttataacaga taattccgaa aaaagggtac aacaaataag cttcttacca ttagtttttt
      841 tgaatgttca atagctaatc agaaacaatt atataaggct cgtttttagt caaaatagca
      901 tgaatcacgt aacaaagctt acgagaaacc gcacctaagg cagttagatg atgttttcct
      961 tcggaaactt ttttctggta aaacgcacta aaaacaggat cacattgaga agctactaaa
     1021 gccgccgaaa acaaggcttt tcgcaaatat ggagaccctc gtttactcat aacattatgc
     1081 gttgcctcat attgccctga ttgtgtcacg gaagcgtcta tcccagcgta agcaacgagt
     1141 tttttcgggt tagaaaattt ttgaatatct ccgatctcac caagaatagt cgcaccgttt
     1201 ataggaccaa ttccaggaat tgtgagaata acagaattta aagtgtccat tatgtgcttg
     1261 atttctcttt ccgtttcctt tatttggctt tcaatgaatt ttaattgttc aatcattgaa
     1321 cgtagttgga aggtgaacgc ttcttgagca aattttactc caaaggagtt agaagctgct
     1381 tgcttcaatt ggtccgcttt ttctgccttg attcggttcc tgcttaactc agctaagtat
     1441 tgggccaaag tttctgagga aatgttttct aagtcgatcg gggcactaaa cgccagcaat
     1501 aactctttag aagcttttcc gaaaacgccc tgcttggtaa aaatcgtcgc gtattctgga
     1561 aagacttgat ccaatacggc tataattttt cttttgaagt cgctcgctgt acctactaaa
     1621 taagttcggt aacgagaaag ttgtttcaaa gaaaaaacat tttcatcgga taagaccgtt
     1681 tcgacaaatg agccatagcg cattaagtca gcaatcaaaa cagaatcaat aatatcgttt
     1741 tttcgtttcc gtatttctgt acctttacgc catccgtctg tttgaagggg attcacaacg
     1801 tgaatcaaat aatctgattc gtgaaggtat gaaaagatgg acagccaata gtgccccgtg
     1861 gcttccatcc ctataacaaa gtcactgggt gctccggaat agtcgaccat tcgctcaatt
     1921 aatgactctg ctccttcagt ggtgttagga aatgaaaaac cttttagtaa tacctttcct
     1981 tcctcgttca tgatagaagc aacgtgagtg cgtttaccga tatcaatgcc taaaaagtac
     2041 atgtatatct gacctttcaa aaaaagaatt ttggattgaa atccactcaa ctgaaaagcg
     2101 tcactacctt gtacgagata cggagtaaag taaggacttt caacatctaa ctcatacgca
     2161 atccacttat cagagagagg agccagtctc tcgagtgcgg gttcaatggc ccaagggggg
     2221 aacggcttag cctctcaatc caataaatat attatctcaa aagaaatgag ttaataccaa
     2281 attaaaataa cgatggttca tagggttatc caatcaaaac cggaaggtta aaaccctaac
     2341 tctaatatac aagggggaat aatatgcttc aaaattttat taatggtctg tatgactttc
     2401 actttttaca gaatgctcta attacatctg tagttatagg gattgtttct ggaacagtag
     2461 gttgttttat tattttaaga ggaatgtctc ttatgggtga tgctatttct catgctgttt
     2521 tgccaggagt agcaatttct tatattttag gtattaattt ttttattggt gcgattgtat
     2581 ttggtctatt agcttctatc attattactt ttataaaaag caatagtgta ataaaaggag
     2641 atacagcaat tggtattact ttcagttctt ttttagcatt aggagttatc cttataggag
     2701 ttgcaaatag ctcaacggat ttatttcata tcttatttgg aaatatttta gctgtacaag
     2761 atattgataa gtggattaca attggcgttt ctgttttagt gttggttaca gttgttctat
     2821 tttttaaaga attattaatc acttcttttg atccattaat ggcaaaagca atggggatga
     2881 atgtcagttt ttatcattat ctattaatga ttctattaac tttagtagca gtaacagcta
     2941 tgcaaagtgt gggaactata ttaattgttg caatgttaat tacaccagct gctacagcat
     3001 atttatatgc aaacagctta aaaactatga tatttatttc ggcgacagtc ggagcgtgct
     3061 catcattgct aggtttgttc attggttata gttttaacat agcagcagga tcaagtattg
     3121 ttttaacagc ggcattctta tttgtaatta gttttctaat ctcaccaaaa caaaaatttt
     3181 taagaaagaa ggagagaaag ttatgatgag aaaatggaaa gcagtactgg gaagtctggg
     3241 gattttgatt gctcttttta tattcggggc atgttcaaca aatagtaaag acaaagatac
     3301 agtggcttcg aacgaaaaat taaaggtagt agttactaat tcgattttag cagatattac
     3361 tgaaaatata gcaaaagata aaattgattt acacagtatc gtacctattg ggaaagatcc
     3421 ccacgaatat gaacctttgc ctgaagatgt tcaaaaaact tcaaaagcag atttgatttt
     3481 ttataacggt gttaacttgg aaactggagg aaatgcttgg tttacaaaat tagtaaaaaa
     3541 tgcgaacaaa gaggaaaaca aagactattt tgcagcaagt gatggcatag atgttattta
     3601 cttagagggt cagagtgaga aagggaagga agatccccat gcttggttaa atttagaaaa
     3661 cggtattatt tacgctaaaa atattgaaaa acagttagcg gaaaaagatc ctgataataa
     3721 aaaattctat aaagaaaatc tagataagta tattgaaaaa ttggattctc tagacaaaga
     3781 agctaaatct aaatttgctt caattccgaa tgataaaaaa atgattgtta caagtgaagg
     3841 atgctttaaa tatttctcga aagcgtataa tgtgccttct gcttacattt gggaaatcaa
     3901 cactgaagaa gaaggaacac cagatcaaat aaaacactta gttgaaaaat tacgcacaac
     3961 aaaagttccc tccttattcg tagaaagcag tgtggacgat agaccaatga aaacagtatc
     4021 aaaagatacc aatattccta tctattcaac gatttttact gattcaattg cagaaaaagg
     4081 acaagatggt gatagttact atgcgatgat gaaatggaac ctggataaaa ttgctgaagg
     4141 cctttcgaaa taatttggag ggtaggtatg gtacaaatta aaacgactac tatactaatt
     4201 tatttactag caattcagga attatcaaaa agaaaaaaga aattaaaaaa taatgattta
     4261 gcaaaagttc taagtgttag tccagcatct gtaagtgaga tgctggctaa acttagatat
     4321 gaggactatt tagatatagg atttcaatta actgataaag ggaaaaaatt tattgacgat
     4381 tacaaaaaag gttttaagta aaagacttta tttttaggga ggataagaaa atggctattg
     4441 gagtgattat gataggactt accctaatag ctggatataa tttttttaaa tctttctcgg
     4501 attattttag aaaaggtgaa tgatttagat gagtctatta attcttgtag gattgctttt
     4561 tgtatcagtt attttgctct ttctcattag tttagctcta aggaatgagt tcagtaatag
     4621 acgttaacga ttgaaacttc aagtcctgtg taagataata tagttaattc atcttattag
     4681 gaggaagtac ccatagaaac cagagattat attacaactc accaaaaatt aatagaaagt
     4741 ggactaagcc attttgaaaa agaaggattt cagcaaacta agataaggca actgtgcttg
     4801 accgcaggcg taactacggg tgctttttat aaacatttta cttctaagga agcgttattt
     4861 gaagaaatta ttcagccgca tattaatgcg cttattaata tgtataatga agagtttacc
     4921 aaatacctct taaacttaga aaatatagat atagagaaaa gttggagaga gaacttcaat
     4981 gaattagatc catttattaa ttatattttt gagaataaac gtatattcga tttggtttta
     5041 ttccaatcag ctggaaccaa atatcaagat attgttgaaa aaattacatc atttgtgaca
     5101 aaaaagacag tctgttgtct agataagtta aagactatcg gaatgatcaa tcaaaattta
     5161 gtctttaatg aagaagaaat ccacttctac atttactcat tctacgctac attctatgat
     5221 atattgaagc atagctattc aaaagaaaag actatgctat taaccagaag attgtatcaa
     5281 ttttctgttc ctgggtggat tgaattactg agttaattaa aaaaaagctt gcattgagaa
     5341 ttgttctcag ttatgctttt ttataagatc gcactgttat ctgaataacg gtgcattttt
     5401 gtcagcttaa gataacagtg cgatcttatt ttttggaggt gaaagagtgt caaaaatcaa
     5461 tccgttaacc ttgattactt taaatatttt ttttccggtc attatttttt taggaaaggg
     5521 aaatatattt caagtggggt gtttcattat tgctacagtt ttagtattga tgtttcgtaa
     5581 aataaagaca tgtatttatt tcatcttgat ttattcagtt ctgtttttag taatgaaaat
     5641 tattgatgct atcaagatag agttttttac tatatttttt gggacatcct tatatattgt
     5701 atttcgaatg ttgccggtcg taatgatctc atggttgttg gttagtgtct atactagcaa
     5761 tgaattatta tcctctttag aaaaaataca tttaccgaaa aaaataatgt taagtattac
     5821 agtagtgatc agattctttc cgacttatcg ttctgaaata agaatgatta aagaatcttt
     5881 aaaaatgaga aacattcgct tgtctttttt tcagcctctg caatatttgg aatttctgtt
     5941 agttcccgtt cttatacgtg caactatgat tgctgaagaa atgaccgcta atgcaataac
     6001 aaaggggata gaatctcctg tgaaaagaag ttcttactat aaaacaaaca tgagcttatt
     6061 agactatatc atcctgtttt ccgttactat ttttttctta agtattttga tttgaggtga
     6121 aagaattgtt tgaatttaac aatgtttctt tttcatataa ggatgttaag gaacaagcgc
     6181 ttgttgacct taatttttca ataaaaaaag gcagtcttac attgattact ggttcttctg
     6241 ggagtgggaa atccacacta ttaaagttaa tgaatggact tattccaaga ctttatgacg
     6301 gaaaattggt tggggaaatt ttatttgaaa ataactcgct acaatcctta acacaagaaa
     6361 gtatatcaaa aaatattggc tacgtaagtc aaaatcctag aggacaattc tttacaacaa
     6421 atacaacgag tgagttagta ttttcgatgg aaaactttgg atttacaaaa gagcatatgg
     6481 ataaaagaat caatgaatta gctgaattat tagatctttc aaatatatta gataaaaaca
     6541 ttttttcgct atcaagtggt gaacggcaga aaatttctat tggatgtagt ttatcgctaa
     6601 actctaaatt aattatattg gatgaaccgt cttcaaattt ggacttcaaa gcgactaaga
     6661 aacttgctga cttactttgt gatttaaaaa ataaaggatt tactattgtt atagctgaac
     6721 atcgttttta ctatttgaaa gaattaattg atcaagttct atttgttaaa aatagaactg
     6781 taaaaactac aactattcag ttgttaaaaa ctgaaaacaa taacgcatta aggaaatttg
     6841 acatttttaa tagttcgatt gaagttccct atatcgaaaa ggtaagtgca gatgtattaa
     6901 aagttgaaga aattacttat gaaaatattc taaaagacat aaattttgat gtcaaagttg
     6961 gagatgttat tggtttggta gggaaaaacg gtgtggggaa aacaacctta ctaagaatac
     7021 tcatgaagat tattcgccct accaaaggaa agataatcga aaataaaaaa aatttgtcat
     7081 cgccgttttt agtaatgcaa gaaatggatt accaattttt cacagaatca gtgaggagcg
     7141 agttagccct ggggaatgaa atagttagta aagatcagca agaaaaaatt ttaaaaaaaa
     7201 tggaacttta taaaatgaaa gatcaattac cgtttgattt atccggtgga gaaaaacaaa
     7261 gattattggt ttctatagct tcattgtcaa aaactaattt atttctattt gatgaaccta
     7321 caagtgggtt ggattatatc aatatggaaa ggattgctga ccttattaaa gacttaaaag
     7381 aaaagggtgc agtagttata gccactcatg atccggaatt tctttataaa acatgcaata
     7441 gaattattta tttaaaaaat ggtggaataa aaaaagatat aaaacttaga gtagaagatt
     7501 caactttgat agaacagatt tttaatgaca taacgaaata gaattgagga gaatatatat
     7561 gaaaacaaaa gattatattt ttattggttt agctacagtt atttccctag taatttactt
     7621 tttttcaata atggttagta gtattggtgg ggcgtttggt cacagcatat ccccagggat
     7681 ttttggatta ttatctggaa tcatatttgt ttatattagc tataactacc ctagaaaagg
     7741 aatttttact atttacacaa ttgttttatt gttatttttt acgttaatgg gcggggcgta
     7801 cttaccttgg tttatcagtt cgattagtat ggcgatctta gctgatatta ttctatctgt
     7861 atttggatat gatcgagcaa ttcctcaagt tgtgtcttgg gttctaatgc aactaggcag
     7921 tgctgccgga cagtggattc ccatatggtt ctttacagat cgttttcgtc aagactggat
     7981 agatagagga caatctgctg caacaatgga tgcaatgatc cactacgcgg tgggtatatg
     8041 gggaattatt tcagtgttag ttgtagcaag tttatctatg attggtgtat tgattggaag
     8101 aaaagtctta aaaaaataca aaaaatagaa cgtagtttac cttctatctg tttgtacatc
     8161 tctaatttac gctattacgg aaagcattaa cagttgtaaa cgaataagag aagaaattga
     8221 atgcggggga ggaaaaaatg aaagtataca aaaaattatt tagttatgtc cctaaaatga
     8281 aatactacgg atggttagcc actttatttt ctgatttttc tgtcgtatta acagtctatg
     8341 gatattacag tatttacaaa tttcttcagt ctctaattat tgtaggagat gaatatttag
     8401 ctaagagtta tgcggttcaa actgttgctt ggttaacttt aggagcttta ttttatattt
     8461 tttctggctt attttctcat atccttgggt ttcgcttaga gactaatttg agaaaaaagg
     8521 gaattagcgg tttaacagaa gcaagttttc gtttttttga tttaaattct tcgggatata
     8581 ttcgaaagac aattgatgac aacgcggcta aaacacatat ggcaattgct cacttgattc
     8641 cagatagtgc tcaagccata gtaacacctg ttttatcagt tgccttaggc tttttcatta
     8701 actttaaaat tggattggtt ctaatcgcca tgttagtaat tggtgtattc ttgttaaaga
     8761 aaatgacaga gaatacaaat tttattcaaa aatatcaaga atcgttagat gtgttaagct
     8821 ctgaaacggt agaatatatc cgcggaattc aagttataaa gatatttggt gctaaagtaa
     8881 catcatttaa agccttgaat aaagcaatta acgattatgc taagtttgca tataagtatt
     8941 ctttgagtgg gaaaacacct tttgttcttt ttcaatggct attttttggc atagtttgca
     9001 ttttgatagt tcctataacc ttttttataa cttctcttac cactcccaag atcttagctg
     9061 ttgacttgtt gatgttgttt tttttaagtg gaattatttt tgtttctttc atgagaataa
     9121 tgtatgtaag tatgcacttg tttgaagcaa gatatgcagt cgaaacacta gactcccttt
     9181 atttagaaat gaagcaagac caactaaaat atgggaacga gacacagatt aaaaattgtg
     9241 acattgcatt tgaaaatgtc agcttttcat acaaagaaga gtgtgtttta gaaaatttca
     9301 acttagtttt agaacaaaaa agaatttatg cattagtagg aaaatcagga agcggaaaga
     9361 gtacgatagc taaactaatt tcaggtttct acaatgtgga taagggacag attaaaattg
     9421 gtggtaagcc tttaaatcac tattcaaaag acacaattat taacacaatt gcttttgtct
     9481 ttcaagatac gaaactattc aatcagagta tttacgataa cgtttctata gcaaatcctc
     9541 aagcgccagc aagtaaagta tttgacgcgc taagattagc cggttgcgaa tcgattttag
     9601 aaaaatttcc cgaaagagag cataccttaa ttggtagtaa aggagtttat ctgtcaggag
     9661 gagaaaaaca acgtatagct attgccagag caattttaaa agatgcaaag attgttatta
     9721 tggatgaagc cagtgcatct attgatccag ataatgagta tgaactacaa aaggcttttc
     9781 aatatttgat gaaagaaaaa acagttatta tgatagctca tcgattatcc agcattaaat
     9841 ctgtagatga aattcttgtc ttgtcagaag gaaaaatagt agaacgtgga aatcataatg
     9901 aattaatggc taaacatcga gtgtataaac aattggtcac taattatgag aatgcaaatg
     9961 attggaggct tgacaatgaa aaatttttat aaaaaaaaat ttgctttaac tgatcagggc
    10021 gcagaagctt taactaaagc ttctatttct agtttttttg tatactgcat taatatggta
    10081 ccagcattca ttataatgat gctgatagac gaattagttt tagaaaatgc aaagccccgt
    10141 tggctttatt ttgcagtatc gtttgttact ttgcttttca tgtattggtt gttagatagg
    10201 gagtatgaaa acttatataa tagtacttat aaagaaagtg cgcatttaag agtgcaaatt
    10261 gcagacgatt tgtcaaattt accattatcc tatttttcaa aacataattt atcagattta
    10321 tctcaaacta tcatgtctga cgttgaaggt attgagcatg cgatgagtca tgcaatacct
    10381 aaagccggcg gtatggctct gtttttccct tttatttcag tgatgctttt ggttggtaat
    10441 gtcaaaatgg gattagctgt tattttgcca acgttattta gttttgtctt aatcttgtta
    10501 tcaaagaaat cccaaacgaa agccaatact aaatattacg atactttgag agaaaactcg
    10561 gaagaatttc aagaaactat tgaattgcag caagagatta atagctttaa tctatctaaa
    10621 aaagttcaag acagactttt caaaaaaatg gaagagagtg aaaggattca tttaaaggta
    10681 gaattaagta ctttttcagt catggcctta tcctctattt tctcatatgt tagtttagca
    10741 gtagtcattc tagtaggtgt tcacttactg ttaacgggtg aagtgactat actctacgtc
    10801 gttggttact tactagccgc aataaaaata aaggattcct tcgattctat gaaagaagca
    10861 gtgcttgaaa tattttattt agctcctaaa atacagcgaa ttagagctat gaaggaaacc
    10921 agtatccaag agggatcaga tagtcctctg aaatcttttg acattgagtt gcgagatgtc
    10981 tctttctcat atgataacaa taccccaatt ttagatcata tctcttttac ggctaaacaa
    11041 ggagaagtga ctgcgttagt aggtgctagt ggttccggga aaactagtat cttgaaatta
    11101 gtgtctagac tgtatgatta tgatgaaggc tgtattttaa ttgatgggta tgatattaaa
    11161 cgtgtatctc cagcttcttt gtttagcaaa atcgctattg tttttcaaga ggtaactttg
    11221 tttaatacaa gtattttgga aaatatacgt attggaaata gtcaagcgtc agatgaggaa
    11281 gtgaaaaaag cagctcgttt agctaactgc gaagacttta ttgaaaaact acctgacggt
    11341 tatcatacat tggtcggaga aaacggaagt tcactttctg gaggagagcg acaacgccta
    11401 tccatagcac gggctttttt gaaaaatgcg ccaattttaa ttttagatga aattactgcc
    11461 agtttagatg cggaaaatga aaaacgtatt caggaaagtt taaatcgttt aatacaagat
    11521 aagacagtac taattatatc gcatcgcttg aagtctattg aaaaggtaaa taaaattgta
    11581 gtgatggatc aaggaaaact agttgatcaa ggtactcata atgagttgta tcgaaggtca
    11641 gaaatataca aaaatctcat aaagaaaact aaactatctg agaaattcgt ctatgaaaaa
    11701 gaagctcaat cacgatgatg aaataggctt taaattaacc tacaatt
//
LOCUS       DAZUMK010000084        11648 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000084 DAZUMK010000000
VERSION     DAZUMK010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.086"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..460)
                     /locus_tag="ACG808_002044"
     CDS             complement(<1..460)
                     /locus_tag="ACG808_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFS5047658.1"
                     /translation="MRCQQHLTYVFLPKYGFYFLLLGVISDLSTPYILGLFYPKLNQM
                     TTVISVFGDVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILAVATVGLY
                     GIGDCIFTGLFSINTNESSWNLSTWIHNTGSGLGYAGFLLFPLLLVLLYRQ"
     gene            complement(500..1552)
                     /locus_tag="ACG808_002045"
     CDS             complement(500..1552)
                     /locus_tag="ACG808_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS5047659.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(1565..2503)
                     /locus_tag="ACG808_002046"
     CDS             complement(1565..2503)
                     /locus_tag="ACG808_002046"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFS5047660.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(2954..3604)
                     /locus_tag="ACG808_002047"
     CDS             complement(2954..3604)
                     /locus_tag="ACG808_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047661.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEVRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(3704..4213)
                     /locus_tag="ACG808_002048"
     CDS             complement(3704..4213)
                     /locus_tag="ACG808_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFS5047662.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILEVFAEAELYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(4576..4959)
                     /locus_tag="ACG808_002049"
     CDS             complement(4576..4959)
                     /locus_tag="ACG808_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5047663.1"
                     /translation="MPKYKEIALALKERIVEGKYQEGSKLPEQESLAREFKTSRVTIR
                     KAIQLLIDEGLLYTRRGSGTFIRSNIRQDNENVTQVNNVFGTSSQENTNVTSKIIRFD
                     VRFPTDYERRLSATYFRSPSCKSWV"
     gene            complement(5200..5814)
                     /locus_tag="ACG808_002050"
     CDS             complement(5200..5814)
                     /locus_tag="ACG808_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5047664.1"
                     /translation="MELAFKQLEKKDYKKAIQFAIKGMHFDWYTKNKLLLNLYGRYFW
                     YLELNRATQIIALYADDELAGVLLADIKGKSKKHHSFSQKLYVKLFDFLQNAFVKDSK
                     GGYDDTNDELLSDYLKKHSPDGEIVFLAANPDLKIKGIGSKLLKEFERREQGKEVFLF
                     TDSGCTYQFYERRGFERKKEKDNVMKILNKEVDLRCMLYSKIIQ"
     gene            5958..6519
                     /locus_tag="ACG808_002051"
                     /pseudo
     CDS             5958..6519
                     /locus_tag="ACG808_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(6630..7604)
                     /gene="lacD"
                     /locus_tag="ACG808_002052"
     CDS             complement(6630..7604)
                     /gene="lacD"
                     /locus_tag="ACG808_002052"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFS5047665.1"
                     /translation="MPLTEKKKTYIRQLSNESGIISALAIDQRGALKKMIGKFQNKSA
                     TDEQIINFKKIISSELTPYASAILLDPEYGLPAAEVRDKNAGLLLAYEKTGYDASTPG
                     RLPDILSIWSVKRLKEQGADACKFLLYYDVDESDDINDQKKAFMERIGSECVAEDLPF
                     FLELVSYDANDPDTSSKEYALKKPHKVNEMMKEFSKPRYGVDVLKVEVPVNMNYVDGY
                     TKNEVAYTKEEAAAYFKEQSETTNLPFIFLSAGVTAELFQETLKFAKEAGSTFNGVLC
                     GRATWANGVEPFVTKGEYAAKEWMQTQGRKNIEELNNVLKETATAIKL"
     gene            complement(7623..8078)
                     /locus_tag="ACG808_002053"
     CDS             complement(7623..8078)
                     /locus_tag="ACG808_002053"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFS5047666.1"
                     /translation="MTLIKTNKVFMNKNFTTQDEVMNFLADQSIVLSISEDRDAVYEK
                     LMEREEESTTGMMDGFAIPHAKDASIKDADIVIVRLNGKVDWKSLDGEGTDFVIALFI
                     PDSEAGTTHLKFLSQVARLLMHKDVTEGLKQAQSAEEIAELLNQKLEEN"
     gene            complement(8106..9551)
                     /locus_tag="ACG808_002054"
     CDS             complement(8106..9551)
                     /locus_tag="ACG808_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFS5047667.1"
                     /translation="MTTYKIVAATGCPTGIAHTYMAQEALEQAAKRKGVSIKVETHGQ
                     VGIENELTPEEIKNADAVIIAADKDVQAERFAGKRVIDVPVSKGIKDADQLIEDALAG
                     KGSITAENKTVDAAGEKAEMGVAAGIGHSIYKNLMNGVSHMLPFVVAGGVIIAISFAI
                     WGVYSFDPNDPTYNPTAAMLKGVGDAAMGMMVPILAAYIAEGIAKRPGLVVGFVGGLL
                     ANSGGAGFLGGIFAGFLAGYFILLLQKVMKGLPKQLDGLKAIFLYPVIGVLFVGVVMS
                     LLANPMEAVNQGMMNFLAGFQNSNPLILGIIVGCMSAFDMGGPVNKAAYVTGTALLAQ
                     GNHTFMAGVSAACIAPPLITGFAALFFGKYFDTNDRNAGLVNFILGSTHITEGAIPFA
                     AKDPLRVLPTMMLGSSIAAVLTYMFGVQVPAPHGGFLVLPVVTGKVAWVGSILLGSIV
                     GGVILGLIQKRSVEKREAEAAAVEMKEATNN"
     gene            complement(9548..10483)
                     /gene="pfkB"
                     /locus_tag="ACG808_002055"
     CDS             complement(9548..10483)
                     /gene="pfkB"
                     /locus_tag="ACG808_002055"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFS5047668.1"
                     /translation="MSVYTCTLNLAIDLFIETDEMKPSQVNRTNDDDIQANGKGVNVS
                     LIMKQLGIDSTALGFKAGFTGNYIEDYLHQKDISTDFISVPGMTRINVFTQVNKTNEE
                     FKLVNKGPEIPQEQIKTFLNQIQKLQADDYLCVSGSLPRGVSPEILIDISRICFENKV
                     HLIIDSSYPEIIDCLPYQPFLLKPNEEELQSWYNVELSDREGYIHYAERLIADGAKNV
                     LLSLGGEGGIFLNKDIVYSGNSPKGKVVNTACAGDTLLGTFLAGYLKGEQVDINLKKS
                     LAAGSSTAFRKGLTDFSDVAELEKQIEIKEGASKK"
     gene            10651..11409
                     /locus_tag="ACG808_002056"
     CDS             10651..11409
                     /locus_tag="ACG808_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS5047669.1"
                     /translation="MDTKLSESEKYLWDFIQSHILEIPSYSIVKLSEYANVSTATVVR
                     TMKKKGYEGFTSFKHSLKEKENTNINFAALDKVDEGIKTAILKNEQEVIRTINMVESG
                     NIEDAIQRIKAARRIMIFARGFSELIAQEMMVKFQLTGKYCEMHTDPNIIKNISRKLS
                     KDDIVIFVSLNGETKELTTAAENCYKAEIGTILLTANRHSSLMEHIEIPFVGFKSEGS
                     FFPDYEVRSRLPLSILARILLDSYALRTSGFTQH"
BASE COUNT         3649 a         2438 c         1809 g         3752 t
ORIGIN      
        1 tttgacgata aagcagaaca agtaacaatg ggaaaagtag aaagcctgca tagcctagcc
       61 cagaacctgt attatggatc caagtcgata gattccaaga gctttcattc gtattgatac
      121 tgaataaccc agtaaaaata caatcaccta tcccatataa accaactgtt gcaacagcaa
      181 gtattgcaag tgtttttgat gttccaacaa ataggtgata caacgcaggc aacgaaagaa
      241 caaaaaaaag tccagataca actgaccaga ccaagaaagc acgtctgact ggactatcta
      301 cgtcaccaaa cacactaatt acggtagtca tttgattcag tttgggataa aaaaggccaa
      361 ggatatacgg tgtcgataaa tcactaatca cacctaataa taaaaaataa aaaccatatt
      421 ttggcaaaaa aacgtaagtc agatgttgtt gacatctcac ttacgcatcc attgattgaa
      481 aatttcttag taaaatctac tagcctaaaa atttgaagac ttcttctcga tcatacagtt
      541 taaatacttt ctcgcataaa aattgagtaa ttagtgccgc aaaaacaggg atgactaacc
      601 aacaaagaac aacaagaaag ccatttagcc ctgcatctaa agaagccaac ggaccaacaa
      661 gtccgaccac accgaaacca gcagaagcag gcgtacctga aatagaaaat agagcgacag
      721 gaatagctgt aatcactgca ctaactaaac aaggaataag aatcacaggt tttctaaata
      781 gattaggcat catcatcttc atagcgccta aagcaatcgc aattgtcaca cctgacttat
      841 taaccttcca agagttcact actaaaacaa aagtggttgc agctacgccc atcgcagccg
      901 caccagcaga aacaccatct agttggattg ccattccgat tgctactgtt gaaataggag
      961 agataatcaa aaaggcaaag gaacaaccaa tcaagataga cattaacagc ggttgtaagg
     1021 tagtgaagtt attaatcaat gatccaattg cagaagtaat tgctgttaca aaagggaata
     1081 aataaaaacc aagtaaacct gcacctacac ctacaacaat cggtgttaac acaatcgaca
     1141 ctgacccaaa actatttcca atcagtaaaa tcagcccgac agcaatcgat gctgtcagca
     1201 tcgtattaat aatatcgcct gtaccagcac caacgtaaac acccgtttca cccaaatcag
     1261 gtgtgaattt gactacacca gaaccaacaa aagaagctcc ggctacaacc atcattctca
     1321 tcggatcaaa tccaaattgt aaggcaatca aaccaccaat gatcaaaggt gttgctaatt
     1381 gaaaaataac tgctaactgt gcgatcgtta ccgcaaaggg atacgcacta aaatatttta
     1441 aaatagcacc taatactgca ttaggaatca atccaataat gattcctgtt gctgtaccag
     1501 cgaggacttt attcagaaaa attttcggtg atattttttg tgaagtttcc attcaatctc
     1561 tcctttattt tacgttcaaa ctatcttctt ttgcatggac aagctcagtc aagaaatcac
     1621 aataaggcgt cgcaacacca tatttcttcc cttttctaga aattgcgcca ttgatataat
     1681 cgatttctgt taatcgatgg ttcttaatca agtcttgata cattgaagga taatgtaacc
     1741 caattgtttc cgggtcaaaa caagattcac aatgtgcaat gacttctggg acatctagtt
     1801 caatcttctc tactgctgct actttggcaa actcattgac aatcgtcgcc accattttat
     1861 gagcagtcga tgtttttcct agctcagcca tattaacgtc taagattgtg catagtccgt
     1921 tcattgttcc gttcacacat gctttgcgat aaatagaata gtgaatattg tcagaaaaat
     1981 gagcatttaa tcctgattca gacaatttgt ctgctaattt ttttgcagct gcttcttttc
     2041 catcacctaa attttgtaat tctactgaac cacttccaaa taatttgacc tgaccaggac
     2101 cttcaagacc tgccgtccac atggtatttc caatatagat attttccatt ggtacaaatt
     2161 tctcaataat atcttcatgc ccgataccat ttaatagaca taagacctct gtatcttttt
     2221 tgattaatga ttggatatcc tgcagcattt tttccagctg catcgctttg gtaaatagaa
     2281 taatcagatc aacttgatct tctttttcta cttcggattg aagaacgatt ggtagttttg
     2341 cttctacctc tttaccatta aaatttgctt gtaatccatg ttctttgatt tgttggacat
     2401 gttctgccca gccatcgatt aataatactt cgttcccact ttggtgaagc attaacccaa
     2461 agcgacttcc cattgcgcca gcgcctgcaa ttgctatttt cattgattct tcctcctttt
     2521 tctttctatt atacttattt gagaatcatt tcacaacctt gttttgttat tcttctctta
     2581 tttatcatgt ttatatccac cattcaagtt attttatcag caaatcaata aaaactggaa
     2641 tagaatccca atttcatcgt aagagaaact agactagcaa taagaaaata gaaacattca
     2701 tagaaaaaac ctcactcctc tgattataaa agaggggaca ttaacctcgt gtatactaaa
     2761 accttccatt cattcgatgg atgattttct ttttgtgaaa aaatattcat tgtagtacga
     2821 tgttgcgcaa cacagacgga tggtattgct tattatttat taaaatcgaa caatacactt
     2881 atttaaaaac ttctttccca gattttcttc catttttaca gttataacta gtattatcat
     2941 gaaaaatagc cttttattgt ctatgggaat aataatttaa aattggtact tttttattaa
     3001 ttagttcatc gcccaataat ctctccagtt tcttagcatc tcctgactca accggaatta
     3061 tccaagccca gtccaaataa ttttttaaaa tctcatcata ttttgcaaat atttcattgc
     3121 catattccgt ccttaaaatt atattttcag tgccagacaa gtgatttgta ttgagttgat
     3181 aaaaaagatc tccatctaaa aaaatatcat tagaagaaga tttccccaca gtaatgacca
     3241 ttccatcagc tctatgtgtt acatacaaat aattgtatga cctcacttca cctaatgaat
     3301 caattatttc ttgcccttta gctctaatat attgatctct attacctatc tcactcttat
     3361 ttaacaaaag ttttttcatg tcaaatttaa tcggctgttt tatttttgta aattcgcttc
     3421 tactactaat ccaatccgag attaattttt caacatgtcc cagagtaaac tctgatttat
     3481 tcgctaaata ctttttttgt tgtattaaag acatctttcc ccgaagctca aaattgagcc
     3541 tacatcgatt ctctatctct tcaaaaagca tcttatcttc atcattctta tattcatcat
     3601 tcataaaacc aatccagctc ctcgattaag ataatatttt aatctcatac aaaaactagc
     3661 tctcatcaaa acaaccattg ttttgatttc tcatgaaatt tctttatact tttctccgat
     3721 tataaattcc taaccccttg agaaaattac gcatatattt atctccgcaa ggcttaaaat
     3781 ttcgttggcc ttcttttctc aaaaatgccc cgatttcgcc ttttgatgga tagagttctg
     3841 cctcagcaaa aacttctaaa atatcatcag tggtcataga caaggcgatt tttaattttt
     3901 ttagcaacac attgttgata tatttgattt ccttgctttt ttgcggcgtg tctactatcg
     3961 gcttatcttt ttgcggacca cgcgccaaga gaatcaggcc atttaggaat gcgtcaaaaa
     4021 cttggttgtt gatggttttt tcatacacat tattttctgc attatctttg ttcttatcct
     4081 gtactttggt cagtaccgcc tgagcttctt ctttagtcag tggtacaccg cctagctcaa
     4141 aagctttaat caaatccgta tctttcaaat caatcgcata acgcaatcgc aataacctat
     4201 cattgttatt catttgttcc tccgaagtat ttagtcttta ctttcttacg tactttttgt
     4261 cttgctaaga tttttaggat caatgataaa aaccgcatca cttatgatac ggagctgggt
     4321 atcatgagtg aatgatattt ataaatcact ggatattgaa gtatattctg ttttctcaac
     4381 ttatttagtc agatttttta atgaagattt tttgagtaaa atttctataa ttcctactta
     4441 ttgaaaagta gtttgtagtg ctgcttctac ctctctcatc tgttctacaa attccaaata
     4501 acttgacgca gcttgcggaa tgaaaaaatg atatgtgaat atttctcctc ctagcccgct
     4561 taatatgcct attttttata cccatgattt gcaggaaggg gatcggaagt aggttgcaga
     4621 aagccgcctt tcataatctg tcggaaaccg gacatcaaaa cggattattt tacttgtcac
     4681 atttgtattt tcttgtgagg aagtgccaaa tacattattt acttgagtga cattctcatt
     4741 gtcctgtctg atattgcttc gtataaaagt accagatcca cgtctcgtat ataacaaacc
     4801 ttcatcaata aggagttgta tagctttgcg gatagtaaca cggcttgttt taaactctct
     4861 agccagactt tcttgctcag gtaatttaga accttcctga tattttccct caacgatcct
     4921 ctcttttaga gccaaggcta tttctttata ttttggcata ttttcccctc atttcattga
     4981 tcctacaaaa aaattagtat atatacaaat ttttgtaaag tatatatact aataaataca
     5041 aaaatatttt tattgttgga tacagaaatc tatttttaac tggggatatc tttgccagct
     5101 aatatctttg agactacacc tatcttcatc taaatggatc aattactcgc tttttccatt
     5161 gaccttcgtg gtacttctgc tttctgatag taaaaaacat tactgaatga tcttactata
     5221 taacatacag cggagatcta cttctttatt aaggatcttc atgacgttat ctttttcttt
     5281 tttccgttcg aaaccgcgtc gttcataaaa ttgatacgta cagccgctat ccgtgaacag
     5341 aaatacttct tttccttgct ctcggcgttc aaattctttt aataatttac taccgatccc
     5401 cttgatcttt aaatcagggt tggcagctaa aaagacaatt tctccatctg gggaatgttt
     5461 ttttaaatag tctgaaagca attcgtcatt ggtgtcgtca tacccgcctt tgctgtcttt
     5521 cacaaatgca ttttgtaaga aatcaaatag tttaacatat aacttttgac taaatgaatg
     5581 atgctttttt gattttcctt tgatgtctgc taataaaaca cctgccaact catcatccgc
     5641 atataaagca atgatttgtg tcgcgcgatt caattctaga taccaaaagt atcttccata
     5701 aagattcaaa aggagtttgt tcttggtata ccaatcaaag tgcatacctt taatcgcgaa
     5761 ttgaatggct tttttgtagt ctttcttttc cagttgctta aaggctaatt ccatcactta
     5821 ttctcctatt ctttaaatat tgatatgcta tacttatatt ataatagaca aggtgtcggt
     5881 tatctatgtt cagatagcgg tgaaattgtc ggataaccga cacaattcct tgattatgtt
     5941 gattaggagg ctgcaaaatg aaaaaagagg atcgccgcgt caaaaaaaca gaaaaagcgt
     6001 tagcagaagc attatcaaaa ttattagtag ataaaaaaat tcaaaacatc accatccgtg
     6061 aactgaccga aactgctgat cttcaccgtt cgacatttta tacacattat acggatatct
     6121 atgatcttta tgaaaaactg gaaaatagct ttttccaaga cttggatgaa atcatttcct
     6181 acgatacgac acatagctac gaggatctat acgctcaatt gatcgactat gtctatacga
     6241 atacgttcgt atacaagtta ttcgtcatca accacggaga tatccaattt caaaagagat
     6301 taaataatct gatcgaagaa aattatttaa aaatctggct acaagaagac aaaaaaaccg
     6361 tgatcacaga agaaatgcgt tacttcacca tctaccatat ccaagggtgc ctctccgttt
     6421 taaatcattg ggtagaaaag caattttgca tactctaaga aaaaaatgct gaccttcttg
     6481 cgcttattga acgacaacat agaacctatc atgccttaaa aacaaacgaa aaaggaagcg
     6541 tcgggacgct tccaattctt tatttaaaag ctcaaattga ttattaaaaa tcattaggtt
     6601 acaaacttaa ggttatagtt aaagctttct tacaatttaa tagctgttgc tgtttctttc
     6661 aaaacattat tcagctcttc gatgttcttg cgtccttgtg tctgcatcca ttctttggca
     6721 gcatattcac ctttagtaac gaatggttct acaccattcg cccaagttgc gcgcccacag
     6781 agtaccccat tgaatgtaga gccagcctct tttgcaaatt tcaaggtttc ttggaacaat
     6841 tcagcagtta ctcctgcgct caagaaaata aatggcaggt tggtagtctc ggattgttct
     6901 ttgaaataag cagcagcttc ttctttggta taagcaactt catttttagt gtaaccatcc
     6961 acatagttca tattaacagg aacttccact ttcaaaacgt caactccata gcgaggctta
     7021 gaaaattcct tcatcatttc atttacttta tgcggttttt tcaaggcata ttccttcgaa
     7081 gaagtatcag gatcgttggc atcataagaa accaattcta agaaaaatgg caaatcttca
     7141 gcaacacatt cagaaccaat ccgctccata aacgccttct tttggtcatt gatgtcatca
     7201 gattcatcta catcgtagta taacaagaac ttacaagcat cggccccttg ctcctttaga
     7261 cgttttacag accaaataga taaaatatcc ggtaaacgtc ctggagttga agcatcgtaa
     7321 ccagtttttt cataggccaa caacagacca gcatttttat cgcgaacttc ggctgccggc
     7381 aacccatatt ctggatctaa taagattgct gaagcatatg gagtcaattc agaagaaatg
     7441 atttttttga aattgatgat ttgttcatcg gttgctgatt tattttgaaa tttaccgatc
     7501 atttttttca aggcaccacg ttgatcgatt gctaacgcac tgatgatgcc actttcatta
     7561 cttagttgac gaatataagt tttctttttt tctgttaaag gcatgatttc actacttcct
     7621 ttttagtttt cttctaattt ttggtttaaa agttcagcta tttcttcagc agactgggct
     7681 tgtttcagac cttctgtcac atctttgtgc atcaaaagtc tggctacctg agataaaaat
     7741 tttagatggg ttgttccagc ctctgaatct ggaataaaca gagcgattac aaaatccgtt
     7801 ccttctccat ctaaactttt ccaatcgacc ttaccgttta accgaacaat cacaatatcg
     7861 gcatccttga tactagcatc tttggcatga gggatggcaa aaccatccat cataccagtc
     7921 gtgctttctt cttctctttc catcaatttt tcgtaaactg catctcggtc ttcactaatg
     7981 ctcaagacaa ttgactgatc tgcaaggaag ttcattacct catcttgcgt cgtaaaattt
     8041 ttattcataa aaactttatt tgttttgatc aaagtcattt gaaatccttc tttctagaat
     8101 aacctttagt tgttggttgc ttctttcatt tcaacagcag ctgcttctgc ttcacgtttt
     8161 tctacactac gtttttggat caaccctaaa attactccgc caacgattga accaagtaaa
     8221 atcgacccta cccatgcaac cttaccagta acaacaggta atactagaaa acctccatga
     8281 ggtgcgggaa cttggacacc aaacatatat gtcagtacag cagcaattga tgaacctagc
     8341 atcatagttg gcagaacacg cagcggatct ttagccgcaa atggaattgc tccttcggta
     8401 atgtgcgttg agcccaagat aaagttgact aaaccagcgt tgcgatcatt cgtgtcgaaa
     8461 tattttccaa aaaacagtgc ggcaaatcca gtaatcaatg ggggcgcgat acaagctgcc
     8521 gaaacccctg ccataaatgt atggttacct tgtgccaaca acgcagtccc tgtaacataa
     8581 gccgctttat tgactggacc tcccatatca aatgctgaca tacagccaac gataatccct
     8641 aaaattaatg gattagaatt ttggaatcct gccaagaaat tcatcattcc ttggttgact
     8701 gcttccattg gatttgctaa taaagacatg actacaccaa caaacaagac accgatcacc
     8761 ggatataaga agatcgcctt cagaccatcc aattgtttag gcaagccttt catgaccttt
     8821 tgcaatagta agataaagta gcctgctaag aaacctgcaa aaattccgcc taagaaaccg
     8881 gctccgccag aattggctaa cagtccgcca acaaacccaa caaccaaacc aggacgttta
     8941 gcgattcctt cagcgatata agctgctaaa ataggaacca tcattcccat tgccgcatca
     9001 ccaacaccct ttaacatagc ggcagtcgga ttgtaagtag gatcattggg atcaaaagag
     9061 tacacacccc agatggcaaa cgaaattgca ataattaccc cacctgccac tacaaacggc
     9121 agcatatgag aaacaccgtt catgagattt ttataaatac tgtgaccaat gcctgctgca
     9181 acccccatct ccgctttctc accagcagcg tcaactgttt tgttttctgc agtgattgaa
     9241 cctttaccag ccaacgcatc ttcgatcaat tggtcagcat ctttaatccc cttgctgaca
     9301 ggcacatcga ttacacgctt acctgcgaaa cgttctgctt gaacatcttt gtcagctgca
     9361 atgattacag cgtcagcgtt ttttatttct tctggtgtta attcattctc tatccctact
     9421 tgaccatggg tttcaacttt gatagatacc cctttgcgct tagctgcctg ctccaatgct
     9481 tcttgagcca tataagtgtg ggcaattcct gttggacatc ctgttgcggc aacaattttg
     9541 taagttgtca ttttttactc gctccctctt tgatttcgat ttgtttttcc aactctgcaa
     9601 catccgaaaa atctgttaat cctttacgaa aagcggttga acttccagct gctaaacttt
     9661 tcttcaaatt aatatctacc tgttcacctt ttaaatatcc tgcaagaaat gttcccaaca
     9721 aagtgtcacc agcacacgct gtattgacta ctttaccttt aggagaattc ccagaataaa
     9781 cgatatcctt atttaaaaat attcctccct ctcctcctag agaaaggaga acatttttcg
     9841 ctccatcggc aatcaagcgt tcagcataat gaatatatcc ttcacgatca ctaagctcga
     9901 cattatacca agattgcaat tcctcctcgt ttggttttaa taagaacggc tgatacggca
     9961 gacaatcaat tatttccgga tagcttgaat caataatcag atggacctta ttttcaaaac
    10021 aaatacgact gatatcaata agaatttcgg gtgagacccc tcgcggcaaa ctgccagaaa
    10081 cacacaaata atcgtcagct tgcagctttt gtatttgatt tagaaaagtt ttgatttgtt
    10141 cctgaggaat ctcaggtcct ttattaacca atttgaattc ttcattggtt ttattaactt
    10201 gtgtaaaaac gttgattcta gtcattcccg gtacgctaat aaaatcagtg ctaatatctt
    10261 tttgatgcaa ataatcctca atataattcc cggtaaaacc tgctttaaat cctaaagctg
    10321 tactatcgat acctaattgc ttcatgatca atgaaacatt cacacctttt ccattagctt
    10381 gtatatcatc atcgtttgtt cgattgactt gggatggttt catttcatct gtttcgataa
    10441 aaaggtcgat tgccaagttt aaagtacatg tataaactga catttacgct cctcctttct
    10501 tacatattca ttatgtatca agatcgaatg taaatgtttt cataaatgac taaattcatt
    10561 gaaaactttt acaaaatata ttaaataaac ttgaagtaaa ggaaactccc gctttacaat
    10621 agaattatcc taactaatcg gaggcaaaat atggatacaa aattaagtga atcggagaaa
    10681 tatctctggg attttattca aagtcacata ctcgaaatcc cctcctattc cattgtaaaa
    10741 ctaagtgaat atgccaacgt ttcaacagca accgtcgttc gtactatgaa gaaaaaagga
    10801 tatgaggggt ttacgtcttt taaacattcg ttaaaagaaa aagaaaatac aaatatcaat
    10861 tttgccgctc ttgataaagt tgatgaagga ataaagactg ctattcttaa aaatgagcaa
    10921 gaggtaatcc gcaccatcaa tatggtagaa agcgggaata ttgaagatgc tattcaacga
    10981 atcaaggctg ctagaagaat catgattttt gcccgaggtt tttcagaatt gattgctcag
    11041 gaaatgatgg tcaaatttca acttaccggt aaatattgtg agatgcatac tgatcccaat
    11101 atcataaaaa acattagtag aaaattatcc aaagatgaca tcgtgatctt tgtttcacta
    11161 aacggtgaaa caaaggaatt gactaccgca gctgaaaact gttataaagc tgagatcggg
    11221 acaattttgc ttactgctaa tcgtcattca agtttgatgg aacatatcga gattcctttt
    11281 gttggcttta aatcagaagg ctccttcttc ccagattatg aggtacgctc cagacttcca
    11341 ctttctatac tggctagaat cttacttgac tcctatgcac tgcgaacatc aggattcaca
    11401 cagcactaac tctatatctg taagactgta cctaaaatat atctgtgtga attaactaag
    11461 gttgtctaga aaaatattta atcgctattt gtattctcgt gctttctatc attgctcctt
    11521 ctataacttc ttagtttcaa gatagaatag tgcattgaga ctaccaggac tacttacgca
    11581 aatatataat aataagaatg tgataagcag ttcgaagttc ctgtctaata tttcttgtta
    11641 attattat
//
LOCUS       DAZUMK010000085        11609 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000085 DAZUMK010000000
VERSION     DAZUMK010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.087"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            116..553
                     /locus_tag="ACG808_002057"
     CDS             116..553
                     /locus_tag="ACG808_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFS5047670.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            629..1249
                     /gene="rpoE"
                     /locus_tag="ACG808_002058"
     CDS             629..1249
                     /gene="rpoE"
                     /locus_tag="ACG808_002058"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFS5047671.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            1438..1944
                     /locus_tag="ACG808_002059"
     CDS             1438..1944
                     /locus_tag="ACG808_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFS5047672.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            1961..2860
                     /locus_tag="ACG808_002060"
     CDS             1961..2860
                     /locus_tag="ACG808_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFS5047673.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            2947..3831
                     /locus_tag="ACG808_002061"
     CDS             2947..3831
                     /locus_tag="ACG808_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFS5047674.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(4023..4094)
                     /locus_tag="ACG808_002062"
     tRNA            complement(4023..4094)
                     /locus_tag="ACG808_002062"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4059..4061),aa:Arg,seq:ccg)
     gene            4154..5488
                     /gene="rpoN"
                     /locus_tag="ACG808_002063"
     CDS             4154..5488
                     /gene="rpoN"
                     /locus_tag="ACG808_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFS5047675.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            5637..6728
                     /locus_tag="ACG808_002064"
     CDS             5637..6728
                     /locus_tag="ACG808_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFS5047676.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            6769..7770
                     /gene="gap"
                     /locus_tag="ACG808_002065"
     CDS             6769..7770
                     /gene="gap"
                     /locus_tag="ACG808_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFS5047677.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            7880..9070
                     /gene="pgk"
                     /locus_tag="ACG808_002066"
     CDS             7880..9070
                     /gene="pgk"
                     /locus_tag="ACG808_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFS5047678.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            9174..9929
                     /gene="tpiA"
                     /locus_tag="ACG808_002067"
     CDS             9174..9929
                     /gene="tpiA"
                     /locus_tag="ACG808_002067"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFS5047679.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            10045..11343
                     /gene="eno"
                     /locus_tag="ACG808_002068"
     CDS             10045..11343
                     /gene="eno"
                     /locus_tag="ACG808_002068"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFS5047680.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         4073 a         2092 c         2449 g         2995 t
ORIGIN      
        1 ctaaaatcaa agagttgttt tggaaaaaag gttttctgta aaaatggaca agttggttga
       61 caaaacaaaa gaaactagga acaatgtagc tatgagaagt aaaggagaat gaataatgga
      121 attatcacaa ggaacaccga tttcaatcaa attgcgtaca aaagtaaaac agcaagggga
      181 agtgcaagat ttttattttg atttgaaagg gcaaatggtg acaattggcg atacgctgta
      241 catacgttat aaagagattc aggaggagac gggcgatgag attcctgtaa cgatcaaatt
      301 agttccagat ggtcatgtac agctgatccg tgcaggagaa atgcggatgc gtttgaaatt
      361 tggctataaa gaacggttag aaacatccta tcgaacacca tacggtatga tccagattgc
      421 tacttttacg aaagaactgc atgttagttt gaaagaccgt cccacaggag ggaaggttcg
      481 aatcgattat gatctgttca tgggaccgga aaaaatcggc gaatattatt tgaccttgga
      541 ttttacagcg taaatataaa agaaaagttt gattttttgt gtctgatttt gtatgataag
      601 gagtagactg tgaaaggacg tgtcctgatt ggaaattagt gtatttgaag gtgcaaacaa
      661 aagcgaatta tcaatgatcg aagtagcaca cgcgatctta gaacaacgtg gcgatgtcat
      721 ggatttttct gatttagcta accagatcca aaactatctt gggaaatcag acagtgaaat
      781 tcgtgattca ctcgcacaat tttatactga cttgaatatt gacggcagct ttatctcatt
      841 aggggataat cgttggggct tgcgttcatg gtatgctatc gattcgatcg atgaagaagt
      901 aaaccatgga atggacgaag aggatgaaga tactccacgt cgccgtaaac gcaagaaagt
      961 caacgccttt atcaatgatg atgaagacgc gatcgattac aatgacgatg acccagaaga
     1021 tacagatctg acagaagaag atgacgatga tctgtttgat gacgatgatg atgaagatga
     1081 agaaatcgct gcttataatt ctgacttgca agaaatcgga gcagatgacc cgacagatga
     1141 tgaagaagac ttgccaggaa atatcgaaga agatttaagt atcatagacg aagacgatga
     1201 tgatgaagat tatgaagagg aagaattttc tgactctgaa aaagaataaa cattattttc
     1261 ttacgagagg ttgtgacaga gacgatcagc ctatcagaat aaggaggtgt gacacgattt
     1321 ttgtcacacc ttcttttttg tatataaaaa tcccaagtaa atacataaaa aataaaaaaa
     1381 ttgaaacttt tagcgcacct tgtacgtcat attaagtgag gcagaaagga gtggagaatg
     1441 atttttactg gatataagaa aaaacggcag atcgaaaaat accgtcacct gctgaatgga
     1501 ctcatcgaaa ctgattatca aaaaatgttc cgtatcacga tgaattacgt acatgataaa
     1561 gaagaggcct tagatgtgat gcaagatagt tttcataaag ccctgaacta ttttgaaaaa
     1621 gaaaaagaga tcgagcattt tagtgcatgg ttttatcgga tattgatccg cacggcatta
     1681 gatagttggc gcagacaaaa aaggacgcct gtggacttat ttgaaattca agatatcccc
     1741 caacagttac ctttagaaac ttccgttgca gagctccata tgattttaga taaaatagac
     1801 agtccggaaa aagagattct gatcctctac ttttttgaag gatttcgttt gaaagaaatt
     1861 gcgactatct tggatatgaa tgaaaacaca gttaaaacga aaatgtaccg ctcattgaat
     1921 gctttacgtc aaatcctaga atgaaggaga gaagagtgcc atgtattccc aaaaagaaaa
     1981 tttaaaaaaa ctaaagaaaa cttataaaga acaagcaatt cccaaagggc ttaaagagga
     2041 gatccgccag cgttttatta tcgaagagca atcatttata aaagcaaagc ggagaaagag
     2101 acgtctccaa gggttcggag tatctttggc tatcgtaatg attacaacaa gtagccttct
     2161 ttttaatagt caagttcgaa gctttgcaga agatttaccc atcttaggat cagtcattga
     2221 attgatctta ggcgaacgct ttactgatcg atctgaaaaa atcgatattc aggtaccgaa
     2281 gatcaataca caaaatgaga aagaaaataa aacaattcat ggattaaatc aaaaatactt
     2341 tcgcgaagga caagcagatt ttgaaaaagc tgaaaaagaa tatggaaatt ttgaaacgga
     2401 tcattaccaa gttttgggag attatcagaa aatcgtagac gataaccggt ttctcgtgat
     2461 tgaaaggcag atcacccaaa cagcagcaga tagccatgtt gaaaaaagat acgacacaat
     2521 cgacaagaaa aacagtgtcc agctctcact tccactttta ttcaaagatg acacctatct
     2581 atctgttttg acaaaagaag tcaaacggca gatggccgaa caagtcaagg aagatccatc
     2641 taagtactat tggactgagc aagatattca agaagggacg atagaaaaac caacacttgt
     2701 tacgcctaca cgaagttttt atttgaacaa agagcatcaa ttagttttga ctttttctca
     2761 atacgaaatt gctcctggtt atatgggtac accagaattt gtcattccta agtccgtgac
     2821 aaaaacgatt ttggcatcgg aagattattt ggatcattag acgagaagta gttgcgacta
     2881 gcagtttcaa ttttcaagta agtacaagta aataataaga agagaaaaag agaaagaaga
     2941 gatatagtga aaagaaatta tttagttatc agtattattg ctttatccgt gcttttgttt
     3001 ttcgaaatta cttcggtggc ctccttgatc accgaagtaa aaggacaaac acactcttcg
     3061 gagcattcgt cacaatcttt caaacaatca gaacaatcaa ccattaaaag ccaatcaact
     3121 gaaacagcta acgtcaaaga agccattcaa gaatcagcgg aaaaggtaca aagtcagtca
     3181 tcacaatcaa cacaagacag tacaaaaacg agccgagatc cggctgtcaa aagcgtcgca
     3241 atcagctttg atgatggacc aggagcgacc acaacaccgc agcttctgcg aatcttaaaa
     3301 gaaaaaaacg tgcatgccac ttttttcgtc ttaggagaaa atacggcgca gcatcctgaa
     3361 atcgtgaagc agacagctga agcagggcac gagattggca atcacacata cgatcatcag
     3421 aatctggcca ctctttctgc ccagtcgatg acggaggaag tgacgaaagc agacactgag
     3481 atcaaaaaag tcactggcaa aacccccact tttgttcgtc cgccctatgg ttctgtcacg
     3541 aatgtaggag caacgatcat ccagcgaccg atcatcgaat ggtctgttga ttcagaagat
     3601 tggaagacaa gaaatcctga tttgattttg cagaagatcc aagcaacagt atacgatggg
     3661 gcaattattt tattccatga tatctatcct gaaacgattc gtgccgttcc tcaagtgatc
     3721 gattatctga aagagcaagg gtatcgcatc acgacagttg gggatttatt agggcatcca
     3781 acggctgtcg agaactatta tggcagaaac gatcatcggc cagttcagta agtgagacaa
     3841 gtatctttaa taaataacac attttaataa gtagttgaac acataactac cttacttgca
     3901 aaggcttaac aatatctata caggtacaac aaaaaactgt ccaccaattt tctttggcgg
     3961 acagttttca cttaaaaata aagaagcaac tattcgtaaa ttatgctaaa atagcagtag
     4021 aacgcgtcct gagggaatcg aacccccgtc tcaagaaccg gaatcttacg tgatatccac
     4081 tacactaagg acgcaagcac tgggataatt gtaactaaac agataaaaaa ttacaagggg
     4141 attggacaaa aaaatgaaat ttgagcaaaa tttttcacaa aaacagcagc agacacagaa
     4201 actagccatg acgcaacagt tgcaacagtc catccaagtt ttacaatata acagtgagga
     4261 gctgttggca ttcgttgaga atcaagcaat ggaaaatcca ttggtggaag ttgttgagcc
     4321 ggaatggcag ccagattaca taaaagcttc ttcatcttct tatgaaggag aagagacaaa
     4381 ttacctaaat cagattcctg atacaaaagg gtctttattt gattcattga tcgaacaagt
     4441 ccatttgaat taccgagata cttttttgag gaaaatcgtt ctttatcttg tagaatacat
     4501 cgatttgaac ggatttttga cgattagttt agaagaagcc ggaaaagaga ttggagctac
     4561 gcctatccaa atgctcgatg cgttgactct gattcagcaa ttagaaccag ctggagtagg
     4621 tgcacgtagt ttgcaagaat gtctcatgct tcaaacagaa cgagatgatt atgcgccgga
     4681 attagcttat atcgtgttgg aagaatgttt tgaggaactg gtggagcgaa aatggaaaga
     4741 aatcgcacaa cgtttttctg ttgatctaca tgctgttcag cagatattcg actatttaca
     4801 aacgttgagt ccaagccctg gccggatttt cgaccgtagc agcgaattgt acattattcc
     4861 tgatgtccgg gtattagtgg acgacgataa aaatgtacaa gttatctcaa accgaaaaaa
     4921 tcagccaaat atacgctttc aagagagtta cttcaaacaa atgtcacaac aagcagataa
     4981 agaaactgaa aattatttaa aagaacggaa acaggaattc gaatggttga agaaaacgat
     5041 cctacaacgt ggggatacaa ttttgcgggt tgctcaagtc attgtttccc ggcaaaaagc
     5101 tttctttatc gacaaggaac gtccaatcaa gcctcttacg ttaaaggaag ttgcaactga
     5161 gatagatgtg catgaatcca cggtcagtcg tgcagtcaac gggaaatatt tggaaacaga
     5221 cttcggtgta tttgaattaa aaaaattttt cactacacga attcctacga atagtacaga
     5281 acagacagaa gacctttcag cagatacagc gaagaaaaag ctgcaggaat tagtcgatca
     5341 agaagacaaa aacaagccgt tgtctgacca gaaattggtt gaattgctga aaaaagatga
     5401 gatagcgatc tcaagacgaa cagtagcaaa atatcgtgat ttattaggaa tcccgagttc
     5461 atcaaaaaga aaacgttatg ataactaatc agtcaaaaga ctagaaaaag tatttcactt
     5521 tttctagtct ttttagttga aaaacgagag caaaactgtt agacttacag tcgtgaggtt
     5581 gagatttccc tttattgatg tgaaattcga atagttaatt tcttttttta tcgtatatgg
     5641 gtcgttttaa gtctacattg gacgtaaagt gtccaactaa ggagatcgct atgctggatg
     5701 aaataaaaat gattgaatct gttgcaccag atattatcga agtgttgcaa gaacgctata
     5761 aaattttacg aaatatctat tggatgcagc cgattgggcg tcgaagttta tcggaaagta
     5821 tggggatcac agaacgagtc ttacgaacag aaacagacct tttgaaaaat ctgaatttga
     5881 tcgacacctc aaaaagcggt atgacgctga caactaaggg cgaagaagtt tatcaaagcc
     5941 ttgaaaactt catggatcag cttttaggta cgcatcaaac cgaacaacag ttggcagaat
     6001 attttggtaa ccaacgctgt atcgttgttt caggaaacag cgaagagcag accaaagtgg
     6061 ctgatgcatt cggcgaagcg ctgagcgaag ccttagaccg actacttcca gaaggcgaga
     6121 atatcatcgc tgtgatggga ggaactacta tggctgtggt agccgaacaa ttgtctaatc
     6181 tggaaaataa gaaacggcat aacctgtttg tccctgcaag aggcgggatt ggtgaagcga
     6241 taactgtaca ggcaaattct gtcagtgcaa gaatggctgc taaagcaaac ggtaaccacc
     6301 gcgcacttta tgttccggaa caattaagtc tggcaactta caattcttta ctcaatgagc
     6361 catctatcca agaagtctta aacttgatta gtgaagcgaa ttgtgttatt catagtattg
     6421 ggcgtgcatt acacatggca gcacgtcgta agatgactga aaaagaatta gtcatgctga
     6481 aacaagcaaa tgctgttgcg gaatcttttg gctacttttt caatgaaaaa ggtgaagtcg
     6541 tttataaggt ccctagaatc ggcatagaat tgcaagattt ggaaaaagtc ccgatcatca
     6601 tggcaatcgc gggcggaaaa tcaaaagcta aagcaattcg agcttatatg aaaaatgcac
     6661 caaaacaaac gtggctcatc actgatgaag ctgctgcaaa tgagatttta aaaggggtaa
     6721 ccctttaaaa taaatatttt tttgattttc ataaggagga aatcttaaat gacagttaaa
     6781 gtaggtatta atggttttgg acgtattgga cgtctagcat tccgtcgtat ccaagatgta
     6841 gatggaatcg aagtagtagc aatcaacgac ttgacagatg ctaaaatgtt ggcacacttg
     6901 ttaaaatacg acacaactca aggacgtttc aacggaacag ttgaagttca tgaaggttca
     6961 ttcaacgtaa acggaaaaga agttaaagta ttagctaacc gcaacccaga agaattacca
     7021 tggggcgaac taggcgtaga tatcgttcta gaatgtactg gtttcttcac ttctaaatca
     7081 gcagctgaaa aacatttaac agctggtgct aaacgcgttg ttatctctgc tcctggcgga
     7141 aacgatgttc caacaatcgt ttataacaca aaccacgaaa cattaactgg taaagaaaca
     7201 gttatctcag gtgcttcatg tactactaac tgtttagctc ctatggctaa aacattgaac
     7261 gacaaatttg gtgttgttga aggattaatg acaactatcc acgcttacac aggtgaccaa
     7321 atgactctag acggacctca tcctaaaggt gacttccgcc gtgcacgcgc tgctgctgca
     7381 aacatcgttc ctaactcaac aggtgctgct aaagctatcg gtttagtaat tcccgaattg
     7441 aacggtaaat tagacggagc tgctcaacgt gttcctgtac caacaggttc attaacagaa
     7501 ttagtaacag ttcttgaaaa agaagtaact gttgacgaaa tcaatgcagc aatgaaagaa
     7561 gcttcaaacg aatcttacgg atacaacaca gacgaaatcg tttcttcaga tatcgttggt
     7621 atgacttacg gttcattatt tgatgctaca caaactaaag taatgacagt tggcgacaaa
     7681 caattagtta aaactgttgc ttggtacgac aacgaaatgt catacactgc acaattagta
     7741 cgtactttag aatacttcgc taacttataa gatagacaaa cgtacaagtg taaattatag
     7801 acatgtacat ttaaaagcgg ggaagcaatc gcgcttcttc cgcttttttt gataataagt
     7861 ttattcagga ggaaataaaa tggctaagaa aacagtgaaa gatatcgatt taaaagacaa
     7921 aaaagttctt gtccgtgttg acttcaacgt tcctttgaaa gacggcgtga tcactgacga
     7981 cactcgtatc aaagcagcat tgccaacaat caactacgtt ctagaacaag gcggaaaagc
     8041 aatccttttc tctcaccttg gacgtgtaaa aacagaagaa gataaagaag gaaaatcttt
     8101 agctccagtt gcaaaacgtc taggcgaatt attaggtaaa gaagtaacat tcgtacctga
     8161 aactcgcgga gaacaattag aagaagcaat ccgcaacatg aatgacggcg acgttgtcgt
     8221 attcgaaaat acacgatttg aagatattga cggtaaaaaa gaaagcaaaa atgatacaga
     8281 actaggtaaa tactgggctt cattaggcga tgtatttgtt aacgatgctt tcggtacagc
     8341 tcaccgtgca catgcttcta acgtaggtat cgcttcaact ggtatcccaa cagttgctgg
     8401 attcttgatg gaaaaagaaa tcaagttcat cggtgaagca gtagaagaac caaaacgacc
     8461 aatgattgca atccttggtg gcgcaaaagt ttctgataaa atcggcgtaa tcgaaaactt
     8521 gattcctaaa gcagataaaa tcttgatcgg tggcggaatg acttacacat tctacgaagc
     8581 aaaaggaatc aagatcggta actctctagt agaagctgat aaagtggaac tagcaaaaga
     8641 attaatcgaa aaagcaggcg acaaacttgt cttgccaatc gacaatgtct gtgcaccaga
     8701 attttcaaat gatgttgaaa cacaagtaat cgaaggagac attcctgatg gtttgatggc
     8761 tttagacatc ggcccagcat cagtaaaatt atttgctgat acattacaag gtgcaaaaac
     8821 agttgtttgg aacggaccta tgggtgtatt tgaaatgagt aactttgcta aaggtacaat
     8881 cggtgtttgt gaagcaatcg ctaacttaga aggcgcaaca acaatcatcg gtggcggaga
     8941 ctcagccgca gcagcagaac aattaggatt tgctgacaaa ttcacacaca tctcaacagg
     9001 tggcggcgca agcttggaat tgcttgaagg taaagaatta ccaggactag cagcaatcaa
     9061 cgataaataa acgaccaagt ttgctcaatt ctgacgagta atcgaacaaa aggtgaaggg
     9121 agttttcatg actccctagc catttttcaa taaattaagg agtgtgttcc tcaatgcgta
     9181 aacctattat cgcaggtaac tggaaaatga acaaaactgc atcagaagca aaagaatttg
     9241 ctgaagcagt aaaaaacaaa atcccttcaa atgacaaagt tgattcagtc atcggatctc
     9301 ctgcattatt cttacaagaa ttaatgacat cagctgaagg tacagaacta aaaatctcag
     9361 cacaaaactg ctactgggaa aactcaggtg cgttcactgg tgaaacttca ccagcagctc
     9421 ttgctgatct aggtgttcat tatgtgatca tcggacattc agaacgtcgt gaatatttcc
     9481 atgaaacaga cgaagacatc aataaaaaag cgaaagctat ctttgcaaat ggtatgacac
     9541 caatcctatg ctgcggcgaa tctttagaaa cctatgaagc aggcaaaact gctgaatgga
     9601 tcgaaggaca aattacagct ggtttagctg atctttcagc tgaacaggtg tcaaacatgg
     9661 ttattgctta tgaaccaatc tgggcaatcg gtactggtaa atcagctgac gcacaaatcg
     9721 ctgacgaaat ttgtggagtt gtacgtcaaa cagttgaaaa actttatgga aaagaagtat
     9781 cagaagcagt tcgtatccaa tacggcggtt ctgtaaaacc agaaaatatt gctgaataca
     9841 tggcaaaaga aaatgttgac ggtgctttag tcggtggcgc aagcttgcaa gcagattcat
     9901 tcttgtcatt gttagacgct gtaaaataat tgttgaaaat ttgcgaaaaa tgcaagaaat
     9961 gtttgcacta atgagcaaaa ttaactaaaa tcgtatatga gggaaatccc ttaggaaaaa
    10021 acaaactcaa aggagagaca aaacatgtca attattactg atgtttacgc tcgcgagatc
    10081 ttagactcac gtggtaaccc aacaatcgaa gtagaagtat acacagaaag cggagctttt
    10141 ggccgcggaa tggttccttc aggtgcttca actggtgaat acgaagcagt tgaattacgt
    10201 gacggagaca aagctcgtta tggcggtaaa ggtgtaacta aagctgtcga caacgtaaac
    10261 aacatcattg ctgaagcaat cattggctac gatgttcgtg accaaatggc tatcgacaaa
    10321 gctatgatcg ctttagacgg aactcctaac aaaggtaaat taggtgcgaa cgctatttta
    10381 ggtgtttcta tcgctgttgc acgtgcagct gctgactacc tagaagtacc tttataccac
    10441 tacctaggcg gattcaacac aaaagtattg ccaacaccaa tgatgaacat catcaacggc
    10501 ggatcacatg ctgataactc aatcgacttc caagaattca tgatcatgcc tgttggcgct
    10561 ccaacattca aagaagctct acgtatgggt gctgaagtat tccacgcatt agcttcaatc
    10621 ttgaaagctc gcggattagc aacttctgtt ggtgacgaag gtggatttgc tccaaacctt
    10681 ggttcaaacg aagaaggttt tgaagtaatc atcgaagcaa tcgaaaaagc tggctatgta
    10741 cctggtaaag acgttgttct tgctatggat gctgcttctt cagaattcta cgacaaagaa
    10801 aaaggtgttt acgtactagc tgactcaggc gaaggcgaaa aaacaactga cgaaatgatc
    10861 aaattctacg aagaattagt ttctaaatat ccaatcatct caatcgaaga tggattagac
    10921 gaaaacgact gggacggatt caagaaatta actgacgtat taggcgacaa agttcaatta
    10981 gttggtgacg acttgttcgt aacaaacact caaaaattat ctgaaggtat cgaaaaagga
    11041 attgctaact caatccttat caaagtgaac caaatcggta cattaacaga aactttcgaa
    11101 gctatcgaaa tggctaaaga agctggctac acagcagttg tatctcaccg ttctggtgaa
    11161 acagaagatt caacaatctc tgatatcgct gttgcaacaa acgctggcca aatcaaaact
    11221 ggttctctat cacgtactga ccgtattgct aaatacaacc aattactacg tatcgaagac
    11281 caacttggtg aagttgcaga atacaaaggt ttgaaatctt tctacaactt aaaaaacaaa
    11341 taattctttt ctaatgaaga tacatgatcg actattgaaa gatagtcgag tatgatcact
    11401 tagttaagta atcgaactct ctacttgctg aaatatcagc aggtagagag tttttttgca
    11461 actagcttct gtatatgatg tttacaaaga atggtacaag aaaagatcat ccttgaaaaa
    11521 ggaaagctaa caataactag catcatataa gagcttttaa ggatagaaca gtagaatagg
    11581 aatctatggg caagtcctct cgtataaat
//
LOCUS       DAZUMK010000086        11506 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000086 DAZUMK010000000
VERSION     DAZUMK010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.088"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(53..2128)
                     /gene="glyS"
                     /locus_tag="ACG808_002069"
     CDS             complement(53..2128)
                     /gene="glyS"
                     /locus_tag="ACG808_002069"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFS5047681.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRVSRGHRFLGEDVTFTHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQITAMALSL
                     ASLDQIITK"
     gene            complement(2130..3047)
                     /gene="glyQ"
                     /locus_tag="ACG808_002070"
     CDS             complement(2130..3047)
                     /gene="glyQ"
                     /locus_tag="ACG808_002070"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFS5047682.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3431..4243)
                     /gene="recO"
                     /locus_tag="ACG808_002071"
     CDS             complement(3431..4243)
                     /gene="recO"
                     /locus_tag="ACG808_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFS5047683.1"
                     /translation="MSQLVESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4386..5285)
                     /gene="era"
                     /locus_tag="ACG808_002072"
     CDS             complement(4386..5285)
                     /gene="era"
                     /locus_tag="ACG808_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFS5047684.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5300..5695)
                     /locus_tag="ACG808_002073"
     CDS             complement(5300..5695)
                     /locus_tag="ACG808_002073"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS5047685.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5679..6155)
                     /gene="ybeY"
                     /locus_tag="ACG808_002074"
     CDS             complement(5679..6155)
                     /gene="ybeY"
                     /locus_tag="ACG808_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFS5047686.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6172..8364)
                     /locus_tag="ACG808_002075"
     CDS             complement(6172..8364)
                     /locus_tag="ACG808_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFS5047687.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEVQEE"
     gene            complement(8388..9359)
                     /locus_tag="ACG808_002076"
     CDS             complement(8388..9359)
                     /locus_tag="ACG808_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFS5047688.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10065..11444
                     /gene="brnQ"
                     /locus_tag="ACG808_002077"
     CDS             10065..11444
                     /gene="brnQ"
                     /locus_tag="ACG808_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFS5047689.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
BASE COUNT         3106 a         2422 c         2121 g         3857 t
ORIGIN      
        1 aaacagagac acagcacgtt tcgtgcatgc tctctgtttt tcaaacaaaa gactattttg
       61 tgatgatttg atccaagctt gctaatgaca aagccatggc agttatttga ctaagttgtg
      121 ttagtcggtt cttacgtact gctggttcat cagccatgac catcgtttga tcaaaataat
      181 tttcgatcag tggacgaaga gcgacaagct ggtcgtagtt ttcgtggatc gtttggtcag
      241 caaaacttgc tttcattgct tctactgctt tgtgtaattc tttctctgcg tcgttttcga
      301 acagctcagg gtctacttct gattgttttt caagctgcgc ttttttcgct aagttcacga
      361 cacgtgtcaa tgcttccatt gaaggtttga agtcttcatc tgcaagatgt tgtttgaaca
      421 attgtgctgc tgcaaatact tttgtcagat ctttttgttc tgcattcaaa acagcttcga
      481 tgacatcatg acggatattc ttcgttgtca ataattggcg cagacggcct ttgataaagt
      541 cgatcacctg ttgttgtccc gctgacaagc caatgccaaa acgatccgta tctttactga
      601 taacttcact aatcgtttct tgcagaacag acaaaggaaa ggcccatttt ttcgattcga
      661 tgattcgaac gacaccatat gcttgacggc gcaaagcata tggatcgttt gagccacttg
      721 ggatcatccc aactgcaaag aaagaaaaga tactatctaa tttatcagca atcgctaaaa
      781 cagaacctac tgtagtctca ggtaaatctc cttcactgga tataggcatg taatgttcac
      841 ggatcgctgt tgctacggct ggtttctcgc ctttaagcag agcatatttt tctcccatga
      901 tcccttgtag ttcagggaat tctcctacca tgttggttac tagatcaaat ttatagattt
      961 cagcagcgcg ttgcagatcc atcaattctt cctcagatag tcctacttgt tcgcctatga
     1021 tgcgagcgat gacttgtacc cgttgcattt tctcatagat gctaccgatt ttttcatgga
     1081 aagtaacagt ttttaatttt tcaacgtatt ggtcgattga agctttcttg tcttcattat
     1141 aaaagaattc cgcatcttct aaacgagcag tcaatacttt ttgattgcct cgtaccacat
     1201 tttccaattt tacccgatcg ccattacgag cagaaataaa atgaggaagc aacatacctt
     1261 gatcgttccg tacttcgaaa taacgttgat gttctttcat agaagtgact agtacttctt
     1321 caggaacaga tagatatttt tcttcgaagt cgccagaaaa gacagttgga tattcgacta
     1381 gattgttgac ttcttctagt aaatcttcat ctagatcaat gatccagtga tttttttctg
     1441 ctaattcgtt tgcttgtgtg atgatcattt cttttctttt ttgcggtgaa gcaataacaa
     1501 attgttcatt caatttttct tcgtattctt ttgcatgcgt aaatgtcaca tcttcgccta
     1561 agaaacgatg acctcttgaa actctgtctg ttttgacatc caagatctca aacgggatta
     1621 tttcgtcatc taacaatgcc acgatccagt gaatagggcg aatatattca aaatcgtatt
     1681 ctgcccagtg catcgttact gggaaagtga ggcttttaat gacatctgct aaacctgtca
     1741 atacttcttt tgcttctttg ccgtgagtgt atttcgttac atagacatat tccacgcctt
     1801 ttaattctct aaaagtgata gcatctgtcg tcagaccttg tccgcggaca aatccttcag
     1861 ctgctttcgt ccagtttcca tctgcgtcca aagcaatttt tttggctggt cctttgactt
     1921 cttcttcgct gtctgcttga cgttctggaa tgtttgttac tttgattgct aagcggcgcg
     1981 gtgtagaaaa cgtttcgatc gtatcataag tcaattggtt ttcatctaaa aattttgctg
     2041 ttttttcttc taattgtttc atgcttggtg tgactacatg agcaggcatt tcttccagcc
     2101 cgatttcaag taataagtct tttgccatct tattcagcct ccttcacgtt ttctggattt
     2161 gctgtgtttt tattcaataa agggaatcct aatttttcgc gttctgccac aaagattttg
     2221 gctacagcac gagccatgtt gcggatacgg gaaagataac ctgcacgctc tgttacggat
     2281 actgcaccgc gagcatctaa taaattgaat gtatggctac atttcaaaat ataatcatac
     2341 gctggatgaa caagattttc atcgatacag cgtttagctt ctttttcaaa acgatcaaaa
     2401 ttgcttagta gcaggtcttg gtcgcttact tcaaaagaat atttagaatg ttcatattct
     2461 ggttgtttga agatctcacc atatttcacg ccatcagccc attccagatc ataaacactt
     2521 tcaacttctt ggatataaga agcaagacgt tctagtccat aagtgatctc agaagtaact
     2581 ggtttacaag gtagtcctcc gacttgctgg aagtacgtga attgtgtgat ctccatccca
     2641 tcaagccata cttcccagcc cacaccggca caacccatcg atgggttctc ccagttgtct
     2701 tcaacaaagc gaatatcgtg ttctaatgga tcaattccta aaagacgcaa gctttctaaa
     2761 taaagctctt ggatattttc aggtgaaggt ttcattacga cttgaaattg atgatgctga
     2821 tacaaacggt ttgggttttc tccataacgc ccgtcagctg gtcggcggga tggctctaca
     2881 taagcagcat tccatggttc cggtccgatt gcccgtaaga atgtatatgg gctcatcgta
     2941 cctgctcctt tttctgtatc ataagcttgc atcagcatgc agccgtttga tgaccaaaat
     3001 ttttgtaatg ttaaaatcat atcttggacg gtcaattttt tactcatctg ttttgcccct
     3061 ttcaattttt aagcgttgct gtcaataaga atacaggaaa gaaaacgaca aaaagtccct
     3121 atgtctgatt acagacatag ggacgaataa tttcgcggtt ccaccctact tccgaataaa
     3181 gattcggcag cttcatttat cgtactcgca agtgcctctt caaaaatcgt ttgggtccgg
     3241 cttccactct cccgaactcg ctaaaccacg tgttttttac tgatcttggt caatgtacgt
     3301 attcattttg cagttttctg gtttgtataa tagatcctct cagtgaacga tctatttaat
     3361 ctacggttaa taatagacgg aagtccctgt atttgtcaaa tagttttgat ttttttgttg
     3421 tatctttcat ttattcttct ttttcaggta tctctttttc aggcatattt ttctctggcg
     3481 tctttaatac gtgtgcccaa gacttcattt gatcaatgaa ctttttgctt tttagatgaa
     3541 taccgacata ttcatcatac aactggtcaa tgaggtcacg gattgcctgc ttcgtttctt
     3601 ctttcaagtg gattccactg atcttatcat aagaaatagc cgaaaacatt cgaacgaaat
     3661 aaacagcgcg aggatctgca tgataacggt gttcgtccat tggccagtgt ttctcgcata
     3721 tcacaccgcc atatttggaa gaatagtcaa attttccttt tgtttccccg catacgacac
     3781 agtgcgtcca aattggcgcg attccgaagc gttgcagtat ttgcacttca aagatattcg
     3841 tgatgatttc tgcatcatct ccttggtcca tccgctgcag agcttgttgc gtaaattgga
     3901 ataaatgcgg gtcatattga tgatcttcga ttgctgcatc tactagattt agcaaatacg
     3961 taccataacc gctcaaaaag atatcttctt ggatgttccg aaacgcatgg acgtctttga
     4021 cactgcttaa aaacgacaaa ccttcacttc gaaaattacc gatatagact gcttcggtaa
     4081 aggggagcaa agccggagct aatggctgat tttgccgatg gattcctttg atataaaaca
     4141 tcattttgcc agaagattcc gtaaagattt tgactaattt atctttttct ttgtaattac
     4201 ggctagataa aatcaagcct ttcgattcta caagctggct catattctcc ctcctaaaaa
     4261 ataaatctgg aaaagcagat tctgccgatc cagattattt atgtttcgat cttccgctgt
     4321 ttttctcaac gtacggattt ttatccagtt tatttctata cctaacctat aagaagtcca
     4381 gaatatcagt actcgtcttt tcggtagccg aagtcttgca gatagacttt tttgtctcgc
     4441 cagtcttttt ggactttgac ccaaagttct agaaatactt tgtctcctaa aagattttcg
     4501 atatcctttc ttgctttcgt tcctacttgc ttcagcattt ttccgccttt gccgataatg
     4561 attccttttt ggctatctct ttcgacgatg atcgtggcct ggatatggat tttatcattc
     4621 tcgtttcgct tcatcgaatc aacaacgact gcgacagaat gaggcacttc gtctcgtgtt
     4681 aaaaacaaga ctttctcacg gatcaattca gaaacgatga agtattcagg atgatcagtc
     4741 acttgatcat ctggaaaata ttgtgggccc tctggcattt cactgactaa tacatccatt
     4801 aagcgttcga cattgtttcc ttctgtagca gagatcggaa tcacttgtgc aaaaggcatc
     4861 tgcgtcgaat aatcttcgat gattcccagc aactcgtctg gatggatctt atcgatttta
     4921 ttgataatca gataaacagg agaatgcacg ttttttagac gttcaataat aaaatcgtct
     4981 ccccggccac gtttttgatc agcactgatc ataaataaga ctgcatccac ttctttcaac
     5041 gcactgtaag ctgcttctac cataaaatct cctagacgat gctttggttt atggattcct
     5101 ggtgtatcga taaaaatcag ctgagcttct ggtgtagtat atactccttg gattttattt
     5161 ctagttgtct gtgccttgtc actcataatg gcaatttttt gacccacgat tcggttcaat
     5221 aatgtggatt ttcctacatt cgggcggccg acgattgcga caaaccctga tttatgttct
     5281 ggcatatacg ttctcctttt taattaaaca acaaatgata tattttaggg acaaataaaa
     5341 tcaacccaat gatgacagca aaacaagcgg tcactaaaac tgctccagct gccatatctt
     5401 tgattttttt cccaatcgga tgaaaatgaa aatccgtaaa catgtccact acattttcaa
     5461 aaacagtatt gatgatctct actatccaaa cgagaaaaac agctaaaagc agccataacc
     5521 actctatccg atcaagctgg aaaatcacgc ccataaataa agcaaggata ccgaacgcaa
     5581 catgtttcct catattgcgt tcttcctcaa aaacggtttt gactccttgg acagcaaatt
     5641 ccacagacat caggaaatgt ttgtttttct ctacgttttt atcttttgag tccataggca
     5701 tccaagatct ctttctgtaa accaaacatt tctttctcat cttctggtgt catatggtca
     5761 tacccgttga tatgcaaaaa tccatgtacc gctaaaaagc caagttcccg atcatatgaa
     5821 tggccgtaag cttctgcttg ttcagcagca cgttcaatag agatcatgat gtctcctagt
     5881 acacgaggta atggattctc tccatcttca aaaatcactg ggatttcgtc ttcgccttct
     5941 tcttccagag caaaactgat gacgtctgtc ggtgcatctt ttccacggta atcacgattg
     6001 attacttgaa ttgccgcatt gtccatgaaa gtaacagaca tttctgtttc ctctggcagc
     6061 tttaaataag atgctgcaaa ttgcagcaag tcatcgattt ctttcatttt ctcttctgag
     6121 acttggtttg tttcatccat aaaggtaata tccattatac atcgcttctt tctattcttc
     6181 ttgtacttcc tttatcttct caacttctgt tttcatttta ggatatttga tccgagaatg
     6241 gaaggtgcta cttaaaccgc tgattaatga tttttccact tttcggattt ctttcaaggt
     6301 cagtccacta tcgtctaatt gaccatcgga aatacgctct tcgataagat tatggacaaa
     6361 actttcgatt ttttcgctcg tcggatgatc cattgcccgt actgcagcct cacaactgtc
     6421 agcaatattg acgatccctg cttcacgact ttgcggtttt ggtccaggat aacggaattg
     6481 ctcttctgtt acttccggat tgcgttcttt cgctttgatg tagaaataac gcattagcgt
     6541 ggttccatga tgctgtttgc aaatatcgat caccatttgc ggcatattat aatcttccaa
     6601 tattttggct ccatctgtca catgtccaaa gataatatgt ttactgtctt ctggcaataa
     6661 gaaattgtgc gggttttccg ctcctgaagg caagttttca acgaaaaagc tagcatgttt
     6721 gatcttgccg atatcatgat aatagcaagc tactcgagtt agcaaagaac gtccgccgat
     6781 ttctgctaca gcgttagcac ttagattagc taccatcata ctatgatgat aagtacctgg
     6841 cgcttcttcc agcaactgtt tcaataaagg atgatttgga ttgctgagtt catttaatac
     6901 aattacactg tcatcattga ccatcaattc aatatatggg tgtagaccga tcgtcagcaa
     6961 gaatgagaag agtgcaccag tgaacccgca gacgagcatt aaccaagtcg ttgcatcacc
     7021 aaagctcatt ccttgataga cgatcaaaat gacatccatc atcacaggaa aaataatgac
     7081 ccacatggca gcagataatc cttgttctga gatccgtttg cgtctgacaa tcgttgccat
     7141 gagcccagag aaaacataag acatcaagat aatcgttaga aagttcgtgc caatcgaatc
     7201 ataaaaaatg aaaagtgcgg atactgcttg gaaaatacca gccatgatcc ctgctcttcg
     7261 attcaagaaa aagcttaaaa caagtggtgc aaaagcagcg ggataaaaca gcgggatata
     7321 agtagcttgt tccgtttgga ataattggaa aaatttcatc agcactatgc tgactgacat
     7381 agctgtcaca tagaaaagca cgtattttgt tctttctcct gcattttgga attgaagtga
     7441 attataaatc agtacgccga tttgaagcaa aacagctaaa accatggcaa ttagcggaaa
     7501 aatcgaagtt gtttgattag tcagtcctag taattccaat tttttcatag ctgaagcatc
     7561 gatttgatta ccttcacgaa caatgatttc tccttggaag atcatcactg gttgtacagc
     7621 ttctcgtgcg gattgtttca attcttctgt ctttttctca ttcaatacgt cattcactac
     7681 gataccttga tccactaagt agcgaatcat ttgttgttgt gctggtgtga cattcaaata
     7741 ttggatttgc tcttctgctt cttgtttaaa agcttccaat tcactttcac gaacttgttt
     7801 gctcatctgt tcgtcaatga gcttcagact ctcatccctc acagtatgaa tctcagtttc
     7861 agtcatttca aatatcgtcg aataaaaatt gttcggtagt ttttgataaa acacaacatt
     7921 ttctgcattc acattttcaa aatttttctt cagcgcggcg atccgctctt ctgtagtagg
     7981 ctttgggata ttttcatctt cctttgcctt atcaatcttt tcgtcataag atttgttgat
     8041 ggcatcattc gtcttatcga tcaattcaaa tagttgcttg atccgattgt tctggtcatc
     8101 tgcaagatct ttttggtaag tgtattctgg agtgactgct tctgctgcca gttttctttt
     8161 ttgttcggtt tcttccgtgt tctcgatcgt tttgttcgca cggatacttt cttctgctac
     8221 ttggccttct ctgaaatcat tggccttttg tttgacacta ctaaatgtaa cgccgcagat
     8281 aatgataaaa aacaagaaca gcaggaaagg caatgctgcc tttccaagtt tttcttgtag
     8341 tttctgcagt gttttattca acatgtgctt atcctcctaa gcaaagctta ttcttgatgg
     8401 agatctgcct tttcataagc tttgatgatt tcggcaacta cagggtgccg aacaacgtca
     8461 cttgcttcaa aattaacaaa atcgattttc ttgatttgct ttaatgttcg ttctgcatga
     8521 acaagaccac ttgtcgtccc tcgaggcaag tctatctgac ttgtatcccc attgacaatc
     8581 atttttgatt gataaccgag acgggtcaag aacattttca tctgagcgac tgtcgtgttt
     8641 tgtgcttcgt ccaagataac gaatgcatca tccaatgttc gcccccgcat gtaagctaaa
     8701 ggtgcgattt cgattacgcc tcgttccatc aatcggttcg tatgatccaa tccaaaaatt
     8761 tggtataaag catcataaac aggccgcaga taaggatcca ctttttcttt tagatccccg
     8821 ggaagaaagc ctagattttc acctgcttcc acagcaggcc gcgtcaaaat gattttttgc
     8881 acttcacctt ttttcaaggc agcgattgct aatactactg caagaaaagt ttttcctgtc
     8941 ccggctggtc ctacgccaaa tacgatatca tgtttggcta ccgattcgac atatttcttt
     9001 tggccgctgt tttttacccg tataggtttt ccattacggt ctttgacgat ctcttcctca
     9061 tacatctcaa caaaataatc taatgtacct ttttgcccca ttttcaatgc cgtcacaaca
     9121 tcgggcgtac ttacgtggat tcctcgtttg atcaattcct gtaatgtccg aatgactgag
     9181 gcagccaagg aaatctggga ttgctcacca atgatctgaa tgatttctcc acgtgtatgg
     9241 atggttgttt ccgttgtttc ctcaatcagt tttatatgct tgtcgtgtgc acctaaaagc
     9301 atgctgacat catcagcagc cgtcaatcta atctctaaag aactagactc ttccgtcaaa
     9361 aatagacatc cccttttttt cgattaattc tttcctattt taccatactc agaagaaact
     9421 atatagcaaa taagaaaagg ttctaaaaga gttgcttaac cataagtacc aagaaacgtt
     9481 aaaaaactag ccgcttccat tctctaaagg tctcgtttga ataaaaaaag ctgatcatgt
     9541 aagctatgca taagatagag gacaagtatt ccgtctaact tagcaagtat tctctgggta
     9601 tttttcttgt ttcaaaaaaa gaaaacaaaa tatttgaaat attcttgcat ttcaaaaaaa
     9661 acaggtgtat tataacgtca acaaataaat atacagcaaa gcgtcgagga aaacagtagg
     9721 ctattcagtg atttattccc agtgagtcat gtaagtgaga aatgacaata aatcgaatag
     9781 acgaacatca tttccgagtc aatgtcgaaa acgtagatca ttgtggctgc ctccattatc
     9841 aggctagcgg ataactttat cgaaaagcgt ccgtgaatga agaagctttt ttaagcgaaa
     9901 aaaggtggta ccgttctaga atctagagcc cttttcccaa gtggaaaagg gcttttttta
     9961 tattttaagg tggtggtttt catggaatct gatattagtg gtatttctag tttctgcaac
    10021 gtttattttg ttactcaaaa tgataattat tggaggaaaa atttatggaa gaaacaaaaa
    10081 cttattcatg gaagcaatta ttgatggtca gttcacttat cttcgggatg ttcttcgggt
    10141 caggtaatct gatctttcct gtacacttag gtcaaatggc tggatcaaac tggatcagcg
    10201 ctggtgtagg atttgccatc tcaggcgctc ttttcccatt attagctatc ttagctgtag
    10261 tagtgacaaa aagtgatggt ctgtatgact tagctaaacc tgtcggaaaa tggtatgctg
    10321 ccttgttctt gatccttgtt catttaacga tcggaccatt ctttggtaca ccgcggacag
    10381 cagcaactgc ttttgaaatg gctgctaaac cctttttgcc tgaaaaatat gattcattag
    10441 gtatgttttt attctctgct cttttctttt tacttgctta tttccttact gtacatccaa
    10501 cacgtttggt caaatatgtc ggaaagtatt taaactcctt gtttcttgca ttattagcta
    10561 tcatttttgt cgtttcttta ttccaaccca tggggaatct aaaccaatct gctgcagatt
    10621 cttatcagac gaacgctttg acaaatggcg ttttagaagg atacaacacg gttgatgcag
    10681 tagctttact tgctttaagt gtcacttttg tccatgcagt tcgtgatctt ggttttaaag
    10741 ataaaaaagt cacagaatta acagcaaaag ctggtagcct gagcgttgcg ttagaagtca
    10801 tgatttattt tggtctggtc cttctcggtg cgatgagttt gaataagatg tctttatcag
    10861 aaaatggcgg aactgcactt tctcagatca ctaattttta tttaggtaac ttcggtgcta
    10921 gcttcttagg ggtaatggtc acattaggtg tcttcactac tgctatggga ctcgttgttt
    10981 cttttgcaca agatttccat aaactgttcc ctaaagtcag ttatatgaca tggctgcggt
    11041 taacgacttt tgtctctttt gtcgttgcga atgccggatt ggataatatc atccaatggt
    11101 ctttaccagt attgatgctt ttgtatccat tgtcacttgc gttaatctta ctttccttaa
    11161 cagcaaaatt cttccaaaag agtccatttg tctatcaagt aacgatgttc ttttctgccg
    11221 ttccagccgt attggatatg ttggcaaatt cgcctgcatt agtgagtcag caaactgtcg
    11281 tagcaagctt actaggattt tatcatcatt atgttccttt tgctgcatta ggtcttggct
    11341 gggtcgtccc aacattgctt ggttacgctg gcagcttgct cttttattat gtatatcgcg
    11401 tatcaggata taaacaagaa gcaagtgagt taccagaaga ataaccaaat caaacacaat
    11461 ttaacataag agatattaaa caggaaaaaa acagctagaa gaaaga
//
LOCUS       DAZUMK010000087        11415 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000087 DAZUMK010000000
VERSION     DAZUMK010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.089"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(111..596)
                     /locus_tag="ACG808_002078"
     CDS             complement(111..596)
                     /locus_tag="ACG808_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFS5047690.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    686..1031
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1076..1717
                     /locus_tag="ACG808_002079"
     CDS             1076..1717
                     /locus_tag="ACG808_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS5047691.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            1736..2371
                     /locus_tag="ACG808_002080"
     CDS             1736..2371
                     /locus_tag="ACG808_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS5047692.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2389..3204
                     /locus_tag="ACG808_002081"
     CDS             2389..3204
                     /locus_tag="ACG808_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS5047693.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3523..4959
                     /locus_tag="ACG808_002082"
     CDS             3523..4959
                     /locus_tag="ACG808_002082"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5047694.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5148..7595
                     /locus_tag="ACG808_002083"
     CDS             5148..7595
                     /locus_tag="ACG808_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFS5047695.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVILLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7825..8892
                     /gene="rlmN"
                     /locus_tag="ACG808_002084"
     CDS             7825..8892
                     /gene="rlmN"
                     /locus_tag="ACG808_002084"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFS5047696.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            8882..9088
                     /locus_tag="ACG808_002085"
     CDS             8882..9088
                     /locus_tag="ACG808_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047697.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(9085..10188)
                     /locus_tag="ACG808_002086"
     CDS             complement(9085..10188)
                     /locus_tag="ACG808_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFS5047698.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10741..11289
                     /gene="aac(6')"
                     /locus_tag="ACG808_002087"
     CDS             10741..11289
                     /gene="aac(6')"
                     /locus_tag="ACG808_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFS5047699.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         3789 a         1933 c         2468 g         3225 t
ORIGIN      
        1 gttcatacta gttgttacat tgtttgattg gccaaaagaa tttctgatat attttgtgct
       61 aaataaagaa aaagacgctt gaattgggaa ataagaaaac tactctatca ttacaaattt
      121 tcataaactg gcagatgttc taagtaaaca agtatctttt caaagtcgga aaaatccata
      181 atcaacgtag tggtattgat atttggatga aagcctacgg tatcttgacg atcaatagca
      241 gcatcgatca cgacttggat ttttttctct ttgtcgtgca tcaatgctaa tggagtcact
      301 gtacctgctg gaacgcctag gaggtcagga agctgttctt cagaaacaaa agataagcgc
      361 ttttctttta attgttcagc tagttgtttt aaatcggcgt gcttttcatc aggcaagatg
      421 acaagataga tttgttttcc ttttcttgat ttcaataata gattttttac ttgcggacca
      481 ggtagggaaa aagggacatt tttgactgac gtaatcgcag gatgatcgac tttttgaaaa
      541 gagataccca gtttttttag tagttcataa gcttcttgtt cggttgcacg actcatcgcg
      601 catcaccctc ctttttttat tagtgtatca taaaaaagag ttgacaaatg aaaaataatc
      661 atgtaaatta cacgtaaagt taaaacaaat gacatcgaaa agaagagtag ccgttaggtg
      721 tttttagaga gtctctggtt ggtggaaaga ggcagcagtt aaccgtgaaa cacatcttgg
      781 agtcgatttt ctgaagcgta tagtaggaaa atccggggag cccgttacag ctgcggtcgt
      841 tgctagatga ccagataagg ctggcttttc cgtgaggagt cggtaaaaaa aggtggaacc
      901 acgtgattaa cgtcctttga tcttcggatc aaaggacttt ttgttttcat ctgaacggca
      961 acgactgtgt gaggtttttt ctgcaaggaa aaaataaaca gaggtggaac cacgtataat
     1021 acgtcctctt ggcatttgcc aagaggattt ttttatttta aaggaggaaa agaagatgga
     1081 gtatttgatt gaagttatgc cgcaattatt agtaggagca ctcacaacat tgaaattatt
     1141 tttatttacc ttgattggct cattaccatt agggattgtt ttagcattag gattgtcttt
     1201 gcattttaag ccgctacgct acttgcttca aacatatgta tgggttatgc ggggaacacc
     1261 gctgttgttg caactgattt ttatttttta tgggttgccg acgattggga tcgtttttga
     1321 tcgtttcgaa gcagcactca ttgcctttat cttaaactat gcagcttatt ttgcagagat
     1381 ctttcgtgga gggatacaat cgattcctca aggtcaatat gaagcagcgc aagttttgca
     1441 attaagtccc tttcaaacgg tgacgaaaat cgtcatccca caagtgacaa agattgtttt
     1501 accttctgta gggaatgaag ttatcaactt ggtcaaagat tcttccttga tctatatatt
     1561 aggtttaggt gacgtaatgc gtgctgggaa aatcgcgatg gaacgcgacg cgactttact
     1621 gcctttgcta ggcgtggcaa tcatctatct tacattgact ggtattctaa caatcgtaat
     1681 gaaacaagtt gaaggaaaat taaattatta ccgctaactg ataggaggaa gaaatatgat
     1741 tgaattgaag aaagtgaaca aagcatttgg tgaaaaaaaa ataatcaaag atttgaatct
     1801 aatcattcca gatgggaaaa cattagctat cgtaggtcct tccggtggcg gtaaaaccac
     1861 gcttttacgt atcttggctg gcttagaaag ttctgacagc ggagaatttt atttagacgg
     1921 tgtaccgttc aacccattcg agcttaaaat gaaagaacaa gttgtaggag tcgtattcca
     1981 agacttccag ctatttccac atctgaatat ttttgaaaac attaccttag caccaaaaat
     2041 ggtattgaaa cagacaaaag aagaatacac caaaaaagca gaagaattag cggcttcatt
     2101 agggattagt gatttattga atatctatcc ttatcagctt tctggcggac aaaaacagcg
     2161 ggtagcgatt gccagagcat tggctatgaa tccacgggta ttggcttatg atgaaccaac
     2221 aagtgcttta gatcctgaat tacgccagca agtcgagcgt ttgatcctat cattgaaaga
     2281 agatggggta acccaaatta ttgtcaccca tgatttagta tttgcagaaa atgtcggtga
     2341 tcaattacta aaagtttccc caattcatta agaaaggaga agatagagat gaaaaaaacc
     2401 agttttctat tcgctttgat cgcaggtctt cttctttttg caggctgttc gaacaaaaaa
     2461 acttcagcag ataattgggc gactatcaac gaagagaaaa aaatcgttat aggtttagac
     2521 gatacatttg ttcctatggg atttagagat aaagacggga agctcacagg atttgatatt
     2581 gatttagcca aagcagtctt tgaacaatat gggattacgg tagactttca gcccattgac
     2641 tggtcaatga aggaatttga actgaacaat ggaacgatcg atttgatatg gaacggctat
     2701 tccaaaacgc cggcaagaga aaagaaagtg caatttacaa aaccttatat ggaaaacgat
     2761 caagtgctga tcacacctaa agaatcagga atcacaaact ttggacaaat gaataataag
     2821 agattaggtg cgcaaaatgg gtcatctggt tatgatgtat ttacgaaaca accagaagta
     2881 ttaaaagaga tagtcaaaaa ccaagatgct gttttgtatg atagttttaa tgaagcattg
     2941 atcgatctga aaagcagacg ggtcgatggt ctgctgatgg ataaagtata cgcagactat
     3001 tatttgaaac aaagaaacga actagaagac ttcaacataa caaaaggttc ttatgagagt
     3061 gaggattttg cagttggtgc acgcaagtca gatagtacat tagtgaaaaa aatcaatgaa
     3121 gccctagatc aactagagaa aacaggtgaa ttccaaaaaa tatccgataa atggtttggc
     3181 gaagatgtta cgccgagaaa ttaacaaata ggggactgtg acaaaagacg tgtcacagtc
     3241 cctttttccg aataaacagt gttctcctgc caactccttg tgactcggag ctgaatggca
     3301 ggctcacaac ctcttggtaa acggtgttca aaagccagtt tttcggtatt aagccaaatt
     3361 tatgcaaaaa tgtagaaagc cattttcaca aaatttttct tactactcga aactaagcga
     3421 ctttttcaca acctccttta cataatttta ttttgttgtg gaaggggttt cgtgttattc
     3481 taaaagagga acgattgaag gttataggaa aggaagagat gaatggaaca caaagaactt
     3541 acctcatttc caaaagattt tctgtggggc tctgcttctg cagcgtatca agtagaagga
     3601 gcttggcaag aagatgggaa aggagaatca gtctgggatc gatttgtacg gatacctgga
     3661 aagacattta aaggaacgaa cggtgatttg gcagtagacc actatcatcg ttataaagaa
     3721 gatattgctt taatgaaaga acaaggtctg aaagcttacc gcttctctgt agcttggaca
     3781 cggatttttc ctaatggacg aggagaagtc aatcaagctg ggctcgcttt ttatgagaga
     3841 ttgatcgacg aattgattga aaacgatatt gaacctatgt tgactttata tcattgggat
     3901 cttccacaag cattgcaaga tgaatataat ggttgggaat cacgtcagat cattgaagat
     3961 ttcactcatt atgcagaaac attatttgag gcattccgcg ggaaagtcca ttactggatt
     4021 agtttgaatg aacaaaatat cttcacttca ttaggttatt tgttagcagc acatccgcca
     4081 ggtgtgacag acccgaaacg aatgtatgaa gttaaccata ttgctaattt agcaaatgca
     4141 tctgtcatca ataaatttca cgagttagaa atgcctggaa aaatcggacc aagctttgcg
     4201 tatacgccaa attatccaat tgacagtaat cctgaaaatg ttctggctgc tgaaaatgca
     4261 gaagacctga tggccaatta ttggatggat gtctatatgt ggggaaaata tccgattgct
     4321 gccatgaggt tcttagaaga aaaaggatgg gcaccaacga ttgaagctgg cgatgcagaa
     4381 ttgctagaat ctgctaaacc agattttctt gggatcaatt actatcaaac ggcgacgaat
     4441 gcattcaatc cattaaacgg agttggtgct ggaaaaatga atacgacagg aaaaaaaggt
     4501 tcatcagaag aaactggagt tcctggaatg tataaaaaag ttgagaatcc atttgtcgaa
     4561 cgaacaaatt gggactggga gattgaccca gaaggattaa ggatcggact gcgtcgtatc
     4621 acaagccgtt accgcatgcc ggtactgatt acggaaaatg gcttaggaga gtatgacaag
     4681 ttgacagaag ataaacaaat ccatgacgac tatcgaatca attacttaca aagccacgtc
     4741 aaagccatca aagaagcaat ttcggatgga gcagaagtcc taggctattg cacctggtct
     4801 tatacagatt tgcttagctg gctgaacggt tatcaaaagc gatatggatt tgtatatgtt
     4861 gatcaagacg aaacacaaga aggatctttg gaacgatata agaaagacag ttattactgg
     4921 taccaaaaac tgattgaaga aaatggagag aacctttagt tcacatactt aaaacaagaa
     4981 ggtacggctg gatatggccg tgccttttct tgataaaatc atttagaaat taagaaaaac
     5041 tgtaaaataa gctgaaaaat gaaagaaagc gtcagttctt tcaaacaaaa ctttagttcc
     5101 cttacttttt tttgtataat gaaacttgat gaaatgaggg ataataaatg gcacaaaaga
     5161 aacggcctac taaaaaaagg aaaaagacga aaaaaacgaa aaagcaaaaa cagcagcagg
     5221 aacaaatgct gacgattggt gttgggtttg ccttgatttt atttgctgtc ttcggctttt
     5281 taaaattagg ttttttagga atattgattg caaacggctt tcggatcatt gcaggaaata
     5341 cttaccaaat cctctgtctt ttattagccg tattaggatt ttggattgtc attaagaata
     5401 cagagttttc aattggtaaa aaccgacgct ggtttggcgg catactcttt tatttcggta
     5461 tcttattatt gttacatgcc catttatttg gcaaactcca tactggggaa ccaaatatca
     5521 tggggacgac atgggatttg ttagccagtg atataaaaca aagtcaagta gacaataacg
     5581 ttggtggtgg gatgattgga gcaatccttt atcattttac ttacttcttg attgcacagc
     5641 ctggaagtta tttagcagca gtgatattac tggcaggagg agcattcctg ttcagtaatc
     5701 tggaaggcta tcagctgtta aatggtatcc aatcaattgg agaacgagta caggaattat
     5761 tggaaggtga tcctgccaag caagctcgaa agcaagcggc aaaggaagag agaatgaaac
     5821 agcgcgcaga agcgaaagaa gcaaggcgct tagctgcaaa agaagctgca gaaaaagaag
     5881 ctgcagaata tgagaagaaa aaagcggtga gccagcaaag ggaaaatact gctgatgaat
     5941 ggcaggaacc agaaaataca gagccggagc agctaagttt tgttccaatc gacagttttc
     6001 aagaaaatat ccatcctgcg aatctagaga aaccagtacc agatacacca aaacagacca
     6061 atacagcaga aggtttttca gatgagttgc cagaggatga cggtacctca ttagaatttg
     6121 agattgaagc agaacaggaa aatcaagact atgaattacc gacagttgat ttgcttgatt
     6181 ctataccaac tgtggatcaa agtgatgagt ataaaaaaat tgaaaaaaat atcggcgtat
     6241 tagaacaaac tttccaaagt ttcggtgtcg atgccaaggt cgtcaaagcc agcttaggac
     6301 catccgtcac aaaattcgaa gtccagccgg ctgtaggggt aaaagtaagt aaaattgtga
     6361 acttaacaga tgatatcgcg cttgctttag cggcgaaaga tgtccggatg gaagcaccga
     6421 ttccaggtaa atcactgatt ggtattgaag taccaaatgg aaagatcagt atggtttcct
     6481 ttagagaaat catcgaagca caaccaaacc acccagataa attactagaa gtacctttag
     6541 gacgtgatgt ttctggacgg gtacaaacgg ctgatctttc gaagatgcct cacttactcg
     6601 tggctggttc gactggtagt gggaagtcag tagcaattaa tgggattatc acaagtatat
     6661 taatgcgtgc taagccccat gaagtaaaac tgatgatgat cgatccgaaa atggttgaat
     6721 tgaatatgta caacggcata cctcatctgt tgacgccggt cgtgacgaac cctagaaaag
     6781 cagcgcaagc cttacaaaaa gtagtacaag aaatggaaga acgatatgaa aaattcgcgg
     6841 caaccggagt aagaaatatt tctggttaca atgaatttgt tcaacagaaa aacttggaaa
     6901 acggaacgaa acatccaacc ttaccattca tcgtagtgat cgtagatgaa ttagctgatt
     6961 tgatgatggt agccagcaat gaagttgaag atgcgattat tcgtttagcg caaatggctc
     7021 gggcagcggg gattcatatg atcctagcta ctcaacgtcc aagtgtggat gttatcacag
     7081 gaatcatcaa agcaaatgta ccttccagaa tggcatttgc ggtatcaagc ggtacagatt
     7141 ctcggacgat cattgattct aatggggcag aaaagctctt agggcgagga gatatgctat
     7201 tcttgccaat gggtgagaat aaaccgatcc gtgtgcaagg agcattcatt tctgaccacg
     7261 aagtagaacg ggtcgttcaa tttgttacag atcagcaaga agctcattat gaagagaaaa
     7321 tgatgccgac agatgaagtt gaaacgacag gtgctccaga acagccacaa gatgagctgt
     7381 ttgaagaagc aaaagcactt gtcgttgaaa tgcaaacggc aagtatttcc cttctccaaa
     7441 gaagatttcg gatcggttac aaccgagcag cacgtttagt agatgaactg gaagctcatg
     7501 gagttgttgg cccgtcagaa ggaagcaagc ctcggaaagt attcattgag caggaagaaa
     7561 catcagaaac gttgccggac gataatgaca tgtaaaactt gtgtgatgaa gggttgaaaa
     7621 acgaataacc aataaaaaga caaggcatga tgataccaaa agaaaaaatg gtttttttcc
     7681 atcttttcta atacggtatg atgtcttgtc tttttagttt ttgtgagatt aaagttctat
     7741 tcgatttatt tgtgaagtat tgcattaagg aaatgaatca tgtatagtat gtagcgaaac
     7801 tttgagaaaa agagggaaaa tactttggaa aaacagtcca tttatggctt gacgaatgaa
     7861 gaattaatca attggtttat agaaaacggc gagaaaaaat tccgagctgc ccaagtttgg
     7921 gaatggttgt atcaaaaacg tgtaagtaat ttcacagaaa tgacgaatct atcgaaacaa
     7981 ttgatcgaaa aattatcgga gcattttatc atcaatcctc taaaacagat ggtcgtccaa
     8041 gaagcttcgg atggtacagt aaaatatttg tttgaactcc ctgataaaaa catgattgag
     8101 actgtactga tgagacaaga atatggcttg tcagtttgtg tgacgacaca agtaggatgt
     8161 aatatcggct gtacgttttg tgcaagcggg ctgctcaaaa agaaccgtga tctgactgct
     8221 ggagaaattg ttgcacagat catgatggtc caacattatt ttgatgagcg caatcttggc
     8281 gaacgcgttt cacatgtcgt tgtaatgggg atcggtgagc catttgataa ttacgacaat
     8341 gtaatggact ttttgcatat catcaatgat gccaaaggat tggcaattgg tgctcggcac
     8401 attacagttt ccactagtgg attagcacac aaaatcaagg aattcgctaa taacggcttg
     8461 caagtcaatc tggctatttc cttgcacgcg ccgaataatg aggtgcgaac atcgatcatg
     8521 cggatcaacc gaagtttccc aattgagaaa ttgatggaag ctgtggatga gtatttagag
     8581 aaaacaaacc gtcgaatcac gtttgaatat atcatgttga atcaagtaaa tgatagacca
     8641 gagcatgcgc aacaattagc agatctatta aaagataaga aaaaattagc atatgtcaac
     8701 ttgattccat ataatccagt cagtgaacat gatcaatatt ctcgcagtcc aaaagctgat
     8761 gtattgagat tttatgatgt attgaagaaa aacggcgtaa actgtgtgat acgtaaagaa
     8821 catggtacag atattgatgc agcttgcgga caattgcgaa gcaagcaaat gaaaaaaaca
     8881 gttgccaaat aatcgtatcc tactcgtttt ttcaattacc ataaaaggag cagttttctt
     8941 aattaaggaa ctgttcaact taaataagag aaaagattct atagcgaaag ctataaagct
     9001 ttttttgaca agaaaaaccc tcatcaagca aagaaatgtc ctcaatttga tgacagcctg
     9061 cttgatgggg gttttagcaa ttatttaact gattttctta tatccttgct ggctttcttg
     9121 tgtagcatga taagtttcta cgtcaagaat ggcctgttta aatttttctc gggtgatttc
     9181 ataaggcaca tgtttaatat catttgttgc catggcttta tctaagattg cttctaaatc
     9241 ggtcgtgaca ttgagattca tgtcggttaa cgatactgga agttggttct ttttcataaa
     9301 cacatagatt ttatcaaaca aatcgaactg ctgatccatc atcaataagg tcagtacacc
     9361 ataactgaca gatgctccgt gaaggtatcc ctccattttt tctagaacag tcgtaccgta
     9421 atagaatgag tgtgccatag cagaattata gttattgtta actaaaacag aaaccaatcc
     9481 ggtagtaaca atgatatccg aaacgacttg ttcaattgca tatgagggac gatcgtcggc
     9541 actatctttc aatgcttgcg taccataagt caagagacga tcaatgcaca tttttccgat
     9601 ttggattcct aatctatccg tgtagcctag atcatcgcca cgagcagaaa aagtagattc
     9661 gacttctttg gataaagcat cgccaatacc tgcccataag tatttatcag gtgcttcggc
     9721 aataattgct gtatcaatga aggtgtgtac aggtggtttt gaaggaaaga agagtccgtc
     9781 cattacgcct tcttcgttat agatgacaca taccgcagtg acgggcgaac agttcgaacc
     9841 aatagtggga aaggtgaaga ctggtttagc tagtttttca ccaatgactt ttacagtatc
     9901 gcaagcacgg ccaccaccga ctgcaaacag gacgtctgct ttttggacac tttcatgctg
     9961 gatcaactgt tcaatatttc cataagttga attacccccg taccagagac tggcgacaac
    10021 ctctatttcc tgtgtcaaag ctacttttat tttgttttct gcctttgaaa gtgcagtctt
    10081 ccctccaatc agcactactt ttgtcccata ttctttgacg attccatttg ctttttcata
    10141 cgcatctgtt ccaatggtat aatttggtaa gaaaacactt tgactcatat tcatttcctt
    10201 ctttcttcgt tgaaacgggt taaaatttta aagagtgatc gatctgactg cggctgtaga
    10261 tggagtagag gtgaacctaa gacaggataa gtgatgaata aggtggcatg aacaccttga
    10321 aatcatcaat aagtatccta aaaacgaaca agtacaaaaa cattgaaaac gacaataatg
    10381 aatttttgtt cctcttagta tttctaccga ttccctttgc ttttttgtaa ttatctaaaa
    10441 attctaaaaa ttaagattat tatatgaaaa caggttttat ttgtcaattc ataattggtt
    10501 ttttttaaag caaaagataa tctatatcat gatgaacaaa cagagttttt tcctccaatt
    10561 gatggctttc ctgatctttc ctattttgaa taaagtgttt ctgtataatt tttttatttt
    10621 ataagtaaga taaataatga aagaatagag atactgattg gtagctaaag atttttgagc
    10681 gagaacattt cgttttttca aaaggactgt tagaataagc aagaacaagg gggaagacgt
    10741 atgataatca gtgaatttga ccgtaataat ccagtattga aagatcagct ttctgattta
    10801 ctgagactga cttggccgga agaatatgga gacagctcgg cagaagaagt agaagaaatg
    10861 atgaatccag aacgaatcgc ggtagcagcg gtagaccaag atgagttagt aggatttatt
    10921 ggtgcaatcc ctcaatacgg tatcacaggt tgggaattgc atccattagt tgtagaaagc
    10981 tcccgacgaa agaaccaaat aggtactcga ttagtcaatt acttagaaaa agaagtagct
    11041 tccagaggag gaatcacgat ttatttaggt acggatgatt tagaccatgg aacaacgtta
    11101 agtcaaacgg acctgtatga gcatacattt gataaagtgg cttccatcca gaaccttcgt
    11161 gaacatccgt atgaattcta tgaaaaatta ggttataaaa tcgtaggtgt cttaccaaat
    11221 gcaaatggct gggacaaacc ggatatttgg atggcaaaaa cgattattcc tcgaccaaat
    11281 tctcaataat aaaaagaggt tgtgacagaa gtaatcagct tcaagaaata agaaggaatt
    11341 tacgaaaatt acttcttgaa tttttgtgaa attccaactt atttccgaag aagctacttt
    11401 tggaacaccg tgtac
//
LOCUS       DAZUMK010000088        11279 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000088 DAZUMK010000000
VERSION     DAZUMK010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.090"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(249..968)
                     /locus_tag="ACG808_002088"
     CDS             complement(249..968)
                     /locus_tag="ACG808_002088"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFS5047700.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1017..2663)
                     /locus_tag="ACG808_002089"
     CDS             complement(1017..2663)
                     /locus_tag="ACG808_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFS5047701.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYFQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            2842..3432
                     /locus_tag="ACG808_002090"
     CDS             2842..3432
                     /locus_tag="ACG808_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS5047702.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3524..5029
                     /locus_tag="ACG808_002091"
     CDS             3524..5029
                     /locus_tag="ACG808_002091"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFS5047703.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5113..6465)
                     /gene="celB"
                     /locus_tag="ACG808_002092"
     CDS             complement(5113..6465)
                     /gene="celB"
                     /locus_tag="ACG808_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFS5047704.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6465..6983)
                     /locus_tag="ACG808_002093"
     CDS             complement(6465..6983)
                     /locus_tag="ACG808_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047705.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(6999..7325)
                     /locus_tag="ACG808_002094"
     CDS             complement(6999..7325)
                     /locus_tag="ACG808_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFS5047706.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7338..9317)
                     /locus_tag="ACG808_002095"
     CDS             complement(7338..9317)
                     /locus_tag="ACG808_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS5047707.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9338..9652)
                     /locus_tag="ACG808_002096"
     CDS             complement(9338..9652)
                     /locus_tag="ACG808_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFS5047708.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            9820..10113
                     /locus_tag="ACG808_002097"
     CDS             9820..10113
                     /locus_tag="ACG808_002097"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFS5047709.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10191..11261)
                     /locus_tag="ACG808_002098"
     CDS             complement(10191..11261)
                     /locus_tag="ACG808_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS5047710.1"
                     /translation="MDFYQSLQLDPFILKQKIREAASKKEKCMYICSLFLRSLFIVLF
                     AICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSIIGLAIVMFSLLVAPY
                     VQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFIVYSLPKDSLNKNFF
                     TQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQEKIWMLIYAFGISLL
                     IVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGSLIGCVLFIGISQFI
                     PFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAVTIRIFENILGVCLG
                     IMYIGVTEILIRKIREKHGLNH"
BASE COUNT         3568 a         2302 c         1928 g         3481 t
ORIGIN      
        1 tcagcgtttt ggcctgagta ttagggagat cgtgtcctta ctatctaact ctgagtcaca
       61 aggaacaatg gggactgtaa caaaagtctt gtcacagtcc cttattccga acaaacggtg
      121 ttccagaagt agcttctacg gaaataagcc gaaatttcac aaaaaagcca tccgaaagtc
      181 agacggacaa gtctgctttt cagatggcgc tacaactact ctgcttattc ttttagctat
      241 ccattcaatt atttttctct cacttgttct agctcttctt tggtcatggg tcgatattgc
      301 cctttattca aatctttagg cagccaaaat tttcccatac ggattctttt taaatataag
      361 acttcttttc ctactgcttt gaccattcgc ttaacttggt gaaactttcc ttcatgcaaa
      421 atcagacgga taattgaagt ttctgtctct tgatctactt gatcaatttg gagttttgcc
      481 ggcaatgtct gctcatcgcc atcaataatc agtccttctg caaataaacg gatatcttct
      541 tctgtcatta taccgctaac ctcagctaga tattctttct ccacatgttt tttaggtgat
      601 agtaactgat gggcaaacac cccgtcattc gttaaaatca agaggccctc tgtgtcttta
      661 tctaatcggc cgactggaaa aagatcttcc cgataatcct ggtcttccaa taaatcgatt
      721 acggtttgat catgattatc ctgagtagca gaaatgactc ctgcaggttt atgcaaaatg
      781 tagtaaaaat ctttttgata cgtaatgatt tctcctaaat aagtaaccgc atcttcgaat
      841 tcatttactt gcgctttatc gcttttcacc acttgtccat tgacttcgat cgttcctttt
      901 ttgattaatc ctttgacttc tttgcggctg ccaagtccca tatctgctaa aaacttatct
      961 aaacgcatgc gtttccccca tttcaaaaga gcatcaatct agctcttctg attcacttat
     1021 tttattttta atttatgtcg taaacgcgct attcctggtc ctagtagtct ttctgccaag
     1081 cggattttca aagctaaata accatacact aaagccccta tacctgctac tacaagaatc
     1141 aacaatagag actggaattt gctatcttcg tttaaaaaag caccaaaaat catctttgtg
     1201 atccacgaag ccaaaagcat aatcaaggta agaataaaga tcagcaatgt cctgcgccag
     1261 acaaaacttg gtttaaagcg ggctacttca tggattctct ttaagatcaa ggtacaagac
     1321 acagcaaatc cgatcattgt cgccaataaa gggccgtata cttcaaataa acgaatggct
     1381 gggtattgaa gaaggagttt cactagaaac ccaataacca aataattaat tgccgccttg
     1441 ttttcaaaca ttccttgcag catgttggaa actagcatat atagtcctag gaagagtcct
     1501 acaaaacttg cttggatcag tacattactt cctagagaat ccggtgtata aaataatgta
     1561 tttagcggat aggcaagtaa cataacacca aatgtcgccg gaaacataaa aaatgaaaac
     1621 aactgtaaat ttccgcttgt caattttgcc agacctactc gatctttcaa cgtaacagct
     1681 tctgtaatca acggtaaccc agtagacgcg atcgatgttg ccaatgcaat aactaccatc
     1741 gtcaacttat ctggattggc actaaaaatc gaaaaaagat ccagtaactg tgcattggaa
     1801 tactcggtgg atgcagacat gatcttcata aaagtgaact gatcgactaa tttgaagatc
     1861 gttacccctg aacccacaat aataaacgga atcgcttctt taacggtatc taacaacaat
     1921 tccttcgttg caatatttat tttgttttcg ctgtagtgaa taaacgccga tgtatacgct
     1981 tgatgtttct tcaaaaaata caaaaggaca agaatacttg ctagcatacc gataaatgcc
     2041 gcgaaagttg attgcgtgac cgccgtcaca tagtcaccat ccatgacttt catgatgatg
     2101 aatgtagcca atagcatata aaaaatacgt gccacctgtt ctacaatctg cgacagcgca
     2161 taaggcatca tttcttgatt gccttgaaaa taccctcgta tcacactcat acaaggaaaa
     2221 accaatacag cgatactcaa agaacgcatc gttggaatca gttcttctcc gccaccagaa
     2281 gcatgagcta gccaaggaga ggcgatgtac atgaacaaag caaacaacag gcctaacccg
     2341 cccatcatct gtaatgcacg gataaataat cgtcgtgaag tgccatattc attcaatgaa
     2401 ttatagcgtg cagtttgttt tgcaatcgca gcaggtattc ctgctgtaga gatcagtaga
     2461 aacaatgcat atatattgta tcccatattg aataatccat tggcagcctt agcattttcg
     2521 cccatccaag cataccaggg aatgatatag acagcgccta gcaatcttga aatgatatta
     2581 ctggcggtca tccaagcaga gcctcgtgcc attttttctt gcggtgttaa ttgatctaat
     2641 gctggttttc tttgattatc catcggtcag attccaactt tctacaaaac attactatca
     2701 ttttaattgg taaaacagaa actggcaatc gattttatat aacttttata agaacaattg
     2761 tttttttgaa ttcggtttcg tttttttggt aaagtaaaga aatatacagc cttttatggc
     2821 taacatttat aggagatatc aatgacacaa gaaataaaaa aactacccgt tatattttta
     2881 ggtcttttct ttatcacagt tggattgaat tggttcttgc tccctcacga tatagcttcc
     2941 gccggagtgg gcgccatcgg acatttagtt gaaacggcct tttctatcca acgctcagtt
     3001 actgtttgga gcatcaatct tatgatgctg cttctagccg ctgtcctatt aggaaaatca
     3061 gtatttttta agactgtagc gggaagtctg ctttttcctg ttgttttaga gattgttcca
     3121 aaagtggaaa tgatctcctc acatttcttt tcactgattt caggaagtct tttgttcagt
     3181 cttggggttt acacattgta cacagtcggg gcctcaaatg gcggtataac gataccgccg
     3241 attattttcc acaaattttt tcggcttcca atgccgcaag gacttttact taccaatagt
     3301 cttatcgtct ttttgaatta ttatgttttt ggattgttgg agacactatt cgttctccta
     3361 tctattacag tcagttcatt gtttatgaaa atactcatcc gcttgtcccc tgtgtcaaga
     3421 aaatttgaat aaagctggat tttgtcatag cggaaacaaa cgatatatta aaaagagtgt
     3481 gctataataa accaagaaat tgaataaagc gagggatcaa tcaatgagct tctctttaga
     3541 agacatccgt catttactat taaaagaaaa tctattaaaa gaatttgttt caacaagagg
     3601 ctggcatctt gatgtaccag aaaaagctga atttcttcag ttaagttatg attcaagaaa
     3661 agctgatgct tctactttgt ttttctgtaa aggaatgaat ttcaaagaag aatatttgga
     3721 ttcagcaatc gaacagggta ttcaatatta tgtatcagaa caaccatacg aaaattcagc
     3781 agtaggaatc atcgtaacgg atatccgtaa agcaatggcg cttcttgcta tggcttttta
     3841 cgactatccg caaaataaat tgaaagtgat cggattcact ggaacaaaag gtaaaacaac
     3901 tgctgcgtac tttacaaaag caatcttgga tcacacaaca aataaaaaaa cagcattgct
     3961 ttctactatg aatacaacgt tagatggcaa aacatatttc aaatcccatt tgacaacgcc
     4021 tgagtcatta gatttatatc gtatgatgtc agaagcagtt gaaaatggta tgactcatct
     4081 agtgatggaa gtttcttcac aagcttacaa aacacaacgc gtatacggct tgactttaga
     4141 tgtcggaatc ttcttgaata tctcgcctga ccatatcagt ccgatcgaac atccgacatt
     4201 tgatgactat ttctattgca agcgacaatt gatcttgaat tcaaaaacag ttgttttaaa
     4261 tcatgaaagt gactattttg atttacttag ggaaacagcc gatctattcc agattccaac
     4321 gatcacttat ggtcgttcgg aagaaagcaa ttatcaagtg atccgttcag ataaaggtac
     4381 tcacggtttt actttatctt ctcatgaaga tcgcttagct atctgcgata ctgcttatga
     4441 tattttgctg gctggaggat tcaaccaaga aaatgcagcc agtgcgatca ttgccgcagc
     4501 actaacaggt gcctccagtc aagatgcaca agacggatta aaagaagctc gtgtaccagg
     4561 ccgaatggat caattgatcc aatccaatgg agcgcatgtc tatgtggact atgcacataa
     4621 ttatctaagc ttgaaaacat tgctgaaatt tgccaaaaat gaacatccag atggccgtgt
     4681 aattgttgta ttaggcagcc caggaaacaa agcaatctcc cgtcgacatg attttggaaa
     4741 agtgctatca gaaacagctg acatcgcctt tttgacggca gatgacccag catttgaaga
     4801 tccgaaaaaa atcgcggaag aaatcaatga agcaattact aatccagatt tgatcgttca
     4861 ttatgaaatg gatcgtcctg aagcgattcg acgcgcttta gcagaaagcg ctccgcaaga
     4921 ttcagtcgtt attgcgggaa aaggcgtgga tccctatcaa aaaatcaatg gtgtagatga
     4981 accttatgaa ggggattatg caatcgtgaa gcggttgatt gaagaataat ggtatttata
     5041 tactagtggt ttcccatttc taagaaaaaa agggactgtg attttttgtc acagttcctt
     5101 ttttcttaga aatcatttta acttttcact agctaattct gctgcctttt ctctttcttc
     5161 atttaataat ttattatcat agaatttgat aaatgggaac cagataatga atgcgatgat
     5221 tgcacaaatg actgctaaca cagcagcttt ccaatcacca ccactaccaa caaatgctcc
     5281 gatcccgact ggtgaaggcc acggcatctg tgcaagcatc ggtttgacga tttccagccg
     5341 aatggcaaaa taagcgagtg tagcagaagc catcggagct aagaaaaacg gaaccgctaa
     5401 atagggattg tagacgattg gcatcccgaa aatgattggt tcattgatat tgaataatcc
     5461 tggtacaagt gaggcttttc ctaacacttt cagctgatca gatttcgcga ggaatgcaat
     5521 aaagatgatc aatcctaaag tcgcacctga tccaccaacg gttacataag aattgttgaa
     5581 ttctcctgcc aacggataat ttgcgccttg tgcgttagct gccatattag ctaatacgat
     5641 cggggtcaag aaagaagtga tgatattcgc tccatggatc cctacgatcc acaatgcatg
     5701 gatcaagaaa taaatcacca tgacaccaag ccatgtattc gtcaactgcg taacaaaccc
     5761 aaatgggata gcaatcattt tgaagatatc cgttcctagt gcaactaata atccattgat
     5821 gatcatcacc acaaacgcaa tgacaaatgt tgggatcaga gcagtgaaag aacgagaaac
     5881 tcctggcgga acaacatctg gcatttttat gacccaattt ctcttgacac acatacaata
     5941 caattcgacc gcaataatcg acataataat agctgtaaag attcctgaag ttcctaaacg
     6001 ttcgacaaaa gagcccatcc taataccatt tactacagtt tctgtctcat tgatagaagc
     6061 aatcaagctg attttaccat cagtcatgat caattcaggg atacacataa agaatgcaaa
     6121 aacagatagt aatgcccccg tgagcggatt aacatttaat ttttgttcat ctcgttcaat
     6181 tgacgttaat tcataagcaa acacaatggc aaagtaaata gataaaatcc ccatcgtcat
     6241 tgtattgacg atcatgtaca agtcactaac tttgaaaaaa gttgcattaa aaaatccttc
     6301 taaaaatggg aacggtaatg gcaaaatatt taaaaccaaa aacatcgagc ctacaatcgt
     6361 gaaaggaata gaagccatcc ccgctgccat gacagcccga acgattttaa gttgcgcaat
     6421 tttccccata ggtcccatca ggtatttctg caaaaaatca aacattagtt attctccttt
     6481 cctaaatata acgctttttc atattgttta atacggatat actgttggtc ttggttgagt
     6541 gaaattttct ttaaacgata ctgtataaat gcactggcaa aaataccgct tacaacaaga
     6601 atgaaaacaa ataactgtcc tccattgtta tttgccataa aaggatacaa gaatgtaaat
     6661 cctgaggtaa acaacactgg aatcactaat aaattaaaga cactctggaa caaaaaatac
     6721 cactttgtcc atggaacgtt cgtttggtgt tttcgataaa gctttacttg ttctcctact
     6781 gcaggcaaaa tagctaaaat cagaatcatc ggtaaactac ttcctaaact tttagctgat
     6841 agaagcagaa tcatccattg catgttcatg aaaaagaaaa aggcagtcag gtaacgtacg
     6901 attagataac gattgaaata catgtttttc aaactaagtt gctttttttc taattcgatt
     6961 ttccttttta ctgtatcctg cacaatgatc cttcctttct atttattttt ttgatacaaa
     7021 taattcattt cctgtgcaac ttccaataat gtcatcgtcg tcatcagatg atcttgtgca
     7081 tggaccatga taatttccat ttctattttt gtcccgctgg catattcttt cagaagttga
     7141 gtttgtgact gatgcgcatg aagaatctct tcatttgctt gtcgcaactt ctcttctact
     7201 tcttcaaaca tctctttacg catcgagcga aatgcttcat gtatcagtgt ccgtgcattt
     7261 ccgctatgga aaatgatatc aaaagaagtc acttgtagtg tttcactgtt tattttttca
     7321 gccatctgtt ttcctcctta ttcgatcagt ttgataaatt tactgctgaa attctcaaaa
     7381 ataggttcag ctaagatttc ctgctgtact tttagctggt caactaacct aacgattgct
     7441 ttagtcacga ccgtaattcc ttcattttcg acataagaag gcgaaactaa aaaaacaaaa
     7501 tgaatttctt gatattgatc ccactgcatc ccagaaggaa tcaatgcaac ggctaccttt
     7561 gtttcgattc caactggaac agcgggatgt gggactgcca ctgtatcact gaatatgatt
     7621 tgtcccatat tttcacgttg ctggatctgc tttcgcattt ctcttatata tccttcttct
     7681 tcatttatgc tcagcttttg cagcaaatca tgaagaattt cttccttttt cggttcgtac
     7741 gtataagtct tgaaaaattt ttcagacaac tgttcggtgt aaatctgctt tttttgctgt
     7801 agagaaaatg ctggtatcgg tctatctgag gaatgatagg gtatagattc tccaactacc
     7861 tttcgtattt tacgaacatc atcttcattc aaaaaaatac tgacatgtaa aactggaatt
     7921 ttgaaaaaca ttgtcgaaag atcaatagaa gaaataatca ggtcgattcc ttttaaagtt
     7981 gtttcattga tctcataata gccttttaca cttaccactg taatattttt accgaattca
     8041 ctgactacgc gattctttaa tagttgggca ctgccgtaac ccgttgcaca gataatcaat
     8101 gcatgtagtt tatgcgcagc tctttctttt tccaatgccg ccatgagatg caaagccaaa
     8161 taggcccatt catcttcatt gatggtatag tttttcaaag ccggcatgtt gctgaaatgc
     8221 ctttttactg cgtcaaagac tttgggattt tctttcttta tctcctcagt caatgggttc
     8281 tcaatgcgaa tacctcggct caatcgaatc atcatcggtg acaaatgatc gagtagtcca
     8341 ttgaaaagct ggagatcctc tactaatgaa aaatgaaatt cctgagacaa tcttttcaga
     8401 actttcgcta attcctttga tagttcctga tcattttgat tttctttgtg attcgactta
     8461 gccatgagat gtaatgtcaa ataagcaatt tcttcttcgg gaaacgatac acctgcgatt
     8521 acctcaatac gttgaacgat ccgctgcgcc acttgatact ctactcgctt agaaacttct
     8581 tgttcgatgc ctaattcctt gatttcaaac ccttctcgga gccgtttgat ccccaaagcc
     8641 aagtgtaaag tcagattctg tatgataaaa tccgaaactt ttaacttagc ttctcttgtt
     8701 tcatcgagaa tgataatgac tagctcttca aaactgatgt ctgggaaaaa acggctttgc
     8761 cctaaatatt ctttcaatga atttccataa tggtttccaa aaaaagtatc catgataaaa
     8821 tgccgtcgat ttctttcttc cccttcgatc catgtccctt gaccatgctt agaaacgatt
     8881 tttagtgaat atggaatcaa tcgctccttg atttcttgca tgtcctttgt caaactggaa
     8941 cgactgatat aaagctcttc tgccaaatca tccataaaaa gaatggcatc ttccaaaagc
     9001 aattggttca atatatactt ttgtcgatct tcggactcat aaaatccttg cggttctcta
     9061 gcatatccag gtactatttc ttgccgactc aaaaacaagt caaatgtcag tctttgtaaa
     9121 atatgcagtt ggtagccata acctggttta gcaatgatct tacctccgtt tttttctact
     9181 aactctttga tccgctgcaa gtaattacgg attgtccgat cagataataa caattcagaa
     9241 gctaaacgtt gactggtgac atactccttc ttgtgatcaa tcaaatatct caacagcaat
     9301 ttttccttct gtttcattat catccactcc tattccatca tatgttctct tcgatcacct
     9361 ctgctaactt ctggatgcct gtagggattg ggacatacgc ttggaaaggg atactaacaa
     9421 caggctttcc aacagattct cctaattttt tgaatttatc caaaaacatc gtcgtttgcg
     9481 ggctgatcag aaataaatca aaatcactat tcttaatcat attatcgcct tctgtagctg
     9541 aaactgcatc tacgagtacc tcttctcttt tttgcttaaa gaaatccgtt gtctttgagg
     9601 ccatcatcga ggatgacatt cctgccgcac aaataatcaa tgcttttttc ataaatcaat
     9661 cactcctttt atttttataa accaagcata tcttgtaagc gttttctatt caaaatatat
     9721 atttccgtag cattgcggaa aaaatgtgta tcatatcttg aataataatt tctggcaccg
     9781 tgatatacta gtgatagaat tttttcagga ggggatttag tgagtatcac agtgaatatt
     9841 ttgtatacaa gtacaaatgg acaagccaga aaatttgcag aagaaatggt cgaaagaaac
     9901 attgtcaaag cgattcgtgc agaagaagga aacgagaaat atgactatta ctttccacta
     9961 gaagatccag aatctctttt actgatcgac cgttggaaag atgaagaagc aatcgaaaag
    10021 catcacaaat ccgaaatgat ggctcaaata aacgaactcc gtaaaaagta taaattaaaa
    10081 atgaaagtcg aacgtttcac tgatttaccg taaaaagata cacatggaat gatagcaatt
    10141 tatcatccat gtgtatcttc actttttcct tattttttat tcgatctcat ctaatgattc
    10201 aaaccgtgtt tttctcttat ttttcggata agtatttcag tcaccccaat atacatgatt
    10261 cctaaacata ccccaaggat attctcaaaa atgcggatcg ttactgctcc tggaaccccg
    10321 aataaactgg cagtgattgc caaagcacca aaagaattaa aaactgtttt atatctatag
    10381 gtagaacaaa cacctaaagc tagaccacct aatataccga cccagttaaa aggaataaat
    10441 tggctgatac caataaataa aacacatcca atcaatgagc caatgattcg gtctactgct
    10501 cgttctttga aaccaatcga catcaatcca tagctagata caatagaaga aaatgcaaac
    10561 cctgcccaca tcaatcgctg aaaagggaca tactctcctg caacgatcaa aagactaatg
    10621 ccaaatgcat agatgagcat ccaaattttt tcctgcgaat acatgccttt caaaaaaatc
    10681 tctttaaaca agcttttgtc tttgttcttt tctcgatgtt ttctgtaaag gagaactgaa
    10741 aaccaacaaa aaaacaaaaa taataagcta cttgtttgtg taaagaaatt cttgttcaac
    10801 gaatctttag gtaacgaata aacaataaag agatatgaaa agccgtacaa agaagcattc
    10861 cccattttgg gatcactagc ggttgccatt aatatgctgc ttagtaaaat aaaatgaatc
    10921 aacatgccca taactgacca tttgatcagc tggacatacg gtgctactaa tagtgagaac
    10981 atgacgattg ctaaacctat gatcgaatgg gaaatcttat aaccaaagtc aacaaaacga
    11041 atactcatca acatacagaa taaaaccaca gcgtatggtt tatgcttcga ttcaaataat
    11101 gttgtaatta taatgataaa acaaatggca aacaatacga tgaataatga tcgtaaaaac
    11161 aatgaacaaa tatacatgca cttttctttt ttagaagctg cttctcgtat tttttgtttt
    11221 aaaataaacg gatctagttg tagagactga taaaaatcca cctgttgtcg tcctttctt
//
LOCUS       DAZUMK010000089        11189 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000089 DAZUMK010000000
VERSION     DAZUMK010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.091"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            151..1410
                     /gene="lysA"
                     /locus_tag="ACG808_002099"
     CDS             151..1410
                     /gene="lysA"
                     /locus_tag="ACG808_002099"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFS5047711.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1397..2197
                     /gene="dapF"
                     /locus_tag="ACG808_002100"
     CDS             1397..2197
                     /gene="dapF"
                     /locus_tag="ACG808_002100"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFS5047712.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2200..3081
                     /gene="dapA"
                     /locus_tag="ACG808_002101"
     CDS             2200..3081
                     /gene="dapA"
                     /locus_tag="ACG808_002101"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFS5047713.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3078..3794
                     /gene="dapB"
                     /locus_tag="ACG808_002102"
     CDS             3078..3794
                     /gene="dapB"
                     /locus_tag="ACG808_002102"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFS5047714.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3800..4495
                     /gene="dapD"
                     /locus_tag="ACG808_002103"
     CDS             3800..4495
                     /gene="dapD"
                     /locus_tag="ACG808_002103"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFS5047715.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4511..5512
                     /locus_tag="ACG808_002104"
     CDS             4511..5512
                     /locus_tag="ACG808_002104"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFS5047716.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5509..6870
                     /locus_tag="ACG808_002105"
     CDS             5509..6870
                     /locus_tag="ACG808_002105"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFS5047717.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6830..7981
                     /locus_tag="ACG808_002106"
     CDS             6830..7981
                     /locus_tag="ACG808_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFS5047718.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7956..9125
                     /locus_tag="ACG808_002107"
     CDS             7956..9125
                     /locus_tag="ACG808_002107"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFS5047719.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9300..10679
                     /locus_tag="ACG808_002108"
     CDS             9300..10679
                     /locus_tag="ACG808_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS5047720.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10960..11189
                     /locus_tag="ACG808_002109"
                     /pseudo
     CDS             10960..11189
                     /locus_tag="ACG808_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3729 a         1952 c         2497 g         3011 t
ORIGIN      
        1 aaccattttt attcttattt ttaaaaaaat atttcgaaat atactagaca attcagaaaa
       61 ttttgtttaa tattattcca ataaaattca aatctttatc aaatgaaatt ccaataaaaa
      121 cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat tgataattgg cggagtcaca
      181 gcaagcacat tggcagaaaa gtataagaca cctctgtatg tgtatgatga agaaaagatg
      241 agacaaacga tgctgtcatt caagcgaggc tttgtatcga aacgttttga tacaaaagtg
      301 ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc atttagttcg tgaatatgga
      361 ttcgggctag atgttgtcag tggtggggaa atctataccg ctttgcaagc tgattttcct
      421 agagatacta tctacttcca tggtaacaac aagacgccag aggaactgat ctatgcactt
      481 gaaaatgatt tgttgcatat tgttgccgat aatttgatgg aagttgaatt attagcagaa
      541 ttaagccaac gctatttaaa aaaaatctgt atcatgcttc ggttgaatgt cggagtagaa
      601 gcacatactc atgaatatat cgtcactgcg catattgatt caaaattcgg catgagctat
      661 gaaagtaaag agtgccaaca agcccttcag ttgataaaag aaagcagcta tctagagtta
      721 gaaggatttc atgctcatat cgggtcacaa atttttgatg ttactgcttg gttagctgaa
      781 atcgacaaat tgacgaatta cttaaccgat tttgaccaac ctctttcctt gaatcttggc
      841 ggagggtttg ggattcgtta tacacaaaat gatcagccga tcccagtcga agaggcacta
      901 aaaaaactga ttgaatatac ggaagaagct ttggaaaaac gaaaactgac cattcgtcaa
      961 ttactgatcg aaccgggtag aagtattgtt ggagaagctg gtacaacact atacacgatt
     1021 ggtttcatca aaaagacacc acataaaaaa tattactttg tagatggcgg gatgacagat
     1081 aatatccgtc cagctctgta tcaggcagaa tacgattgtg atttagcagc aaaactgtcg
     1141 gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt gtgaatcagg cgatgtggta
     1201 attaaagaag cttatttgcc gaaagcagaa tcgggtgatc ttttagctgt ttattccacg
     1261 ggagcttatg gatactcgat gagcagcaac tacaatcgag cgacacgacc agcagttgtt
     1321 tttgtcaata atggccaatc aagactgatt gttcgcagac aagcattttc tgatctacta
     1381 aggggggaag tttcctatga tccttcataa atataatggc tgcggaaatg attttatctt
     1441 attagattac gcagaagaca cagattatag tcaattagcc gcttctttat gcacaaaaga
     1501 aaaatatgat atagacggat tgatcgccgt aaaaatcgat ccgttggaaa tgatctatta
     1561 caacaaagat ggcagccgcg cgccgatgtg cggaaatggc attcgctgtt ttgctcgata
     1621 cgtaaatgat cagaacatga tgaatgaaca ggaattcaac gtagagacat tagcaggcac
     1681 aagaaaaatt acggtgttgg atccctcacc tttttattgc agcgtagata tgggagtgcc
     1741 agatgattct ccggaaaaaa tcaaagctaa tcaaacttat ccaataaaaa accaggaaat
     1801 agagatcaat ggtgaaaaag taacgatcac tagtttattt ttaggaacga ttcataccgt
     1861 tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa agaggtgaac tgatctgtca
     1921 tcatcctttg ttcaaagaaa aaaccaatgt caactttgta gaagtagtca atgaacaaga
     1981 attgatcgtc cgtacctatg aaagaggagt aggttggaca cttgcttgcg gtacaggttg
     2041 ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt tcagcagatc aggtagttgt
     2101 tcacttggaa caaggagact tgatgatctc tggtgaggat gcgatacaaa tggctggccc
     2161 agcagttcat gaatggacaa gaatattgga ggaatgaaga tgataaaagg ttcgatcgtg
     2221 gcgctgatca cgccgatgaa tgaagaggga tcagtagatt atgctggttt ggaaaaattg
     2281 atccaattcc atttagatga acaaacagat ggattattag ttttaggtac aacaggagaa
     2341 tcttctacgc tcacacaatc tgaggaggag cagatccttc aactgacagt aaagaaagtg
     2401 gctggccgtg tgccggtgat tgcaggagct ggaacgaata atacgaaaga aacgattgaa
     2461 aaagcgaagc attttgcttc tttgggggca gacgcacttt tggtcatcac cccttactac
     2521 aacaaaacaa gtgatgcagg actagctgct cattttacag caattgcaga agcctcaccg
     2581 attccgctta ttttgtataa tgttccttct agaacaggca tgtcgattcc aatccatgta
     2641 ttagtaaatt tagcagaaca tcctaatatc attggtttga aagaagcctc aggagatatg
     2701 gcttatgtga tggatgccgc tcggttgatt ggagaagaat tcttccttta cagtggaaat
     2761 gatgatttga tcctaccagt aatgagtgta ggaggaagcg gtgtaataag tgtatgggcg
     2821 aatatccaac ctaaaatagt ccatgaatta gtaaaagata cgcaggacgg aagatggcag
     2881 caagcaaaag aaaagcaatt aaatgcactt gaactgatcc atgccttgtt cagtgagacc
     2941 aatcctattc ctgtcaaagc agccatgtca ttattagact taccttccgg acctctacgc
     3001 ctgcctttag tttctctttc aaaagagaaa aagaaacaat tagctcagct tttgttaaaa
     3061 gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag tggacagatg ggaaatagag
     3121 tcgaagaagt tgcacaagaa agaggagata cggtccttct tttacggacg gcacctaaag
     3181 aagcaactac gcttgctttt acggaactgc cagaactgct tattgatttt tctcatccag
     3241 ataatttaga gaaaatcctt tcgtatagct tatcatatca acttccgtta gtcattggca
     3301 cgacaggtta tacacaatca cagttccaag aaataaaaga acatgccaaa catgtacctg
     3361 tgatgtatag tgctaatttt tcgttcggca tcatggtgat gaatcaacta atcaagcaag
     3421 cagctgcaat gttgcaaggc tggcagatcg aactgattga aaaacatcat agtcgtaaaa
     3481 aagatgcacc atctggtact gcaaatatgc tgcttcaaac aatccaagaa gtgcaaaagc
     3541 tgcagcccgt atacaactgg caagggaaaa aaagagaaga aaaccaaatt ggtgtacaca
     3601 gtattcgtgc aggttcttta cctggtgaac atgatgtatt gtttgctgct actgatgaaa
     3661 tatttacgat caaacatgaa tctttttcta atcgaatctt tgctgcaggc gcagtagaag
     3721 cagcggactg gttgaaagat cagtcaccag gttattacaa attagaagat ttattaatgg
     3781 acaaaggggt gtaaaaaacg tggaagctca agaaatcatc cgctacattc aaacggcatc
     3841 taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag ccaatcgaat ttaaagaaag
     3901 taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt gactatgaga cgattgctcc
     3961 ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa atcgaaactg ctgcaagata
     4021 cagcgcagtt cctttgctag atattaaaaa aattaatgct cgtatcgaac caggtgctat
     4081 tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc atgatgggag cgattatcaa
     4141 tattggtgca gtaatcggtg aaaacacgat gattgatatg ggtgctgtct taggtggacg
     4201 agcgacagtg ggcaaaaact gccatattgg tgcaggagct gtcttagcag gtgtgatcga
     4261 accagcatcg gccaaaccgg ttatcgtaga agacggcgta ctggtaggtg cgaatgcagt
     4321 gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg gcagcaggag cagtggtttt
     4381 agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct gcacgtgtat taaaaataag
     4441 tgatgatcag acaaaagaaa atacagcatt gattgctgcg ttacgcgaat tatagaggag
     4501 atggagaaaa atgcgcgaaa tagatacctg tattgtgggg gcaaccggat tagtcggcca
     4561 aacgatgctc aaagtgttag aagaatatga ttttccagta ggacgattga aattattagc
     4621 ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa aagaaacaca ctgtacagga
     4681 attgacagaa acttcatttg aaggatatga tttagcgcta ttttctgcag gtgcttctgt
     4741 atccaaagag tttgcgccaa tcgcacgaag tcagggacta tttgtcatag ataattcctc
     4801 agcatggcgg gaagattcgg atattccatt aatcgttcca gaggtcaatc tggaagacta
     4861 tacattgaat cggctgattg caaatccaaa ttgttcaacc attcaagcgg tacttccgct
     4921 taaagcttta caagaagcct ttggtatcca gcgagtcaat tattcgacct atcaagcagt
     4981 ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt acgcaaaatg acgaggaacc
     5041 agttttgtat ccttataata ttagccaaac ggtgattccg gaaatcgatc agcctttgga
     5101 aaatgggtat acaaaagaag aacagaaaat gataaatgag acccgtaaga ttctgcatca
     5161 gcctgctttg ccagtatcag caacctgcgt acgtgtgcca atcgaaaatg gacatggagt
     5221 ctctgtggca gttcaactgc aaaaagagtt tacgacggaa gaaattagag agtgtctcag
     5281 ccagtttcca gggattcaat tagaagatga tctagcagca cataaatatc cgacgagcat
     5341 tcttgctaga ggaacagata tggtttatgt tgggaggatc agaaaagaca cttctttaga
     5401 gaacggattg ttgttctata cgactgccga taacatacgc aaaggagccg ctgcgaatgc
     5461 cgtacagatt gctgcagctt taatgcagaa aatagcggag gtggcaagat gagtaatacg
     5521 ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg aagagcaatt caaaaaagtc
     5581 cgtacaattg ttctttcaga tcccaatcgc caagtgatcg ttgtttctgc tttaggcaaa
     5641 cgtacgtcag atgatgacaa agtaacagac ttactttatt tgatttatgc ttaccttcag
     5701 catcatgttg attggacacc cctttggaaa aggatttgtg atcgtttcat tcaagtaaga
     5761 gatgttcttg ccctgcagtt acctattgaa gatgaactag cacgcataca gaaacaactg
     5821 gaagacgaaa agataactga agattattta gtaagtagag gtgaatatct gactgctcaa
     5881 ctgatggctg agtatcttgg ttaccagttt attgacgcaa aagacctgat tttctttgac
     5941 tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa tcacaaaagc atttaaaaga
     6001 tatccgaaaa tggttgttcc agggttttac ggggcgaacc ctgcaggaaa agaaaaatta
     6061 ttgggccgtg ggggaagcga cattaccggc gcgatactgg cgaagatgtt gcaagcggaa
     6121 agttatgaga attggacgga tgtttcagga atcatgatgg ctgatccgcg aatcatcgat
     6181 catcctaaaa tcatcgagga aatctctttt agagaactga gagagatggc ttatatgggg
     6241 gctaacgttc ttcatgaagc agctgttttc ccagttcaag aggctgatat ccccatccaa
     6301 ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa tatcgaatca cgaaatagaa
     6361 aaggaaaatg gactgacagg aatagctgga cgcaaggatt ttctttccat cactttattc
     6421 aaacgtcata tgtccgatga aatcgggttt atctggaaag caatgagcat ttttgctaaa
     6481 catgagatat caatcgaaca tattccttca gggatcgaca atatcggagt gatcgtctca
     6541 gcagaagcga ttgccgataa gttatttctc atcacgaaag aattaaaaga aacacttggc
     6601 gtggaagaaa tcgtcgtgat cgaagactta gctttgatct ctgtcgttgg gggtccgcat
     6661 aaagaattga ttggattatc tgggaaagtg ttatcgatat tgaacaaatt agaaattcgc
     6721 acatcgatct tatcacaagg agcacaggag ttgaatctga tcatcggtgt accgaataat
     6781 caatatgaaa cagtagtgaa gggtatctac gaagggatgg tgaatacgga tgctgaaaga
     6841 atatcgaatg gcactccatc aaattcctga aatcgggttt catgaattca agacaaaaaa
     6901 atatttatat gaacaagtaa aaaacagcga ggccgttatc catgaaatcg gcgaaacagg
     6961 actactcctt tattttgata atcagcaaga agaaacgatt gcttttcgaa cagatatcga
     7021 tgcactgcca gtcaaagaag aaacgaatct ctcttttgct tctaagcatg aaggttatat
     7081 gcatgcatgt ggtcatgacg gacatatggc gatgctttta gggttgactg actttataac
     7141 taaacacatt gaggatctta tttataatgt agtactgatc tatcagccat cagaagaaat
     7201 ttccggtggc gcacaaagcg ttattcagtc aggattgttg gataaatatg aagtacaagc
     7261 aatatttggc tttcatctat ggcctggact agctaaggga gaagttttct ctcgaccagg
     7321 acctttgatg gcacaaagca gtgaaacaga cattatcatt aaaggccagt ctgcacatat
     7381 tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct gtacgatttt tgaagaatgt
     7441 ttatgatttt gaccggcaat taccggcaga gtggaatcat ctaatgaaat ttggccaaat
     7501 ccaaggtgga acgatccgaa atgttttagc tggagaagtt attgtatccg gaagtatccg
     7561 ttcttacagt tcagatatgc aaaattattt gaagagggga ttagaccatt tagcagaaca
     7621 gtttcactct acagctacta tttcttttcg ctataatgac ggctatccag ctgtaatcaa
     7681 ccatagaggc ttatatgcag cactgttaga ttcaaacaaa ctacatgaat tagatgctcc
     7741 agttcttcaa gccgaagatt ttggcatata tacgcaacaa tatccatgtg tatttttctt
     7801 tttaggtgtc ggtgataccc ctgctttaca tgatccgacg ttcgattttg atatggatgt
     7861 tttagaaaat ggtgtagaat ggtacaaaac gattcttttt acagaaaatc ttcttgtaaa
     7921 aatcgatagc agttcaaaag aggagcgagt aaataatgat gaatttatcc aatcgattta
     7981 gtcctaggct ttctagaata gaagtatcaa aaatacgaca attcgatcag caaatatcgt
     8041 cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga ttttccaact cctgaacatg
     8101 tcaagcaagc aggaatagca gcgattgaag aagacttttc tcactatacc ggaatgagag
     8161 gattagagga actgcgggaa gcggcttgta tatttcagca acagcgttat ggattaactt
     8221 atgatccgca aacggaagtt ttgacgacag ttggtgcaac ggaagcgatt gcctctgcgt
     8281 tgttatctgt tttggaagaa ggagacaagg ttcttattcc agcgcctgct tattcaggat
     8341 atcagccatt agtggaacta gctggtgctg aattgatccc gatagatact tctgacacag
     8401 ggtttgtctg tcagccggaa caatttgaac gagcatttga acaatatgga tcagctgtca
     8461 aagcagtcat tttaaactat cctaacaatc ctactgggac gacattgtct gcaaaacaat
     8521 tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt tgttataagt gatgaagtat
     8581 atgctgaatt gacttatagt ggaacgcata tgtccatagc tacttatctt ccagaacaaa
     8641 caatcgtgat aagcgggtta tccaagtcac atgcgatgac tggttggcga gtgggcttta
     8701 tttttgctca aaaagccctt attgacgaac taattaaagt ccatcaatat ctggtgactg
     8761 ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct aataaatggg gtgaaagata
     8821 gcgagaatat gaaagagcaa tatcaaacac gaagagatta tctggtccag caattggcgc
     8881 ctttaggatt tgaagtgacc cagccaaacg gtgcatttta cttgttttgc aaactcccag
     8941 ctgccattca aactgactct tggcagtttt gtgtagaatt agcggaacag gcaaaagttg
     9001 cgtgtatccc aggaattgca tttggcccag aaggagaagg ttatatccgg atttcttatg
     9061 ctagcagcat ggaaaaatta catgaggcct gcaaacggat caaagacttt ttaacagaac
     9121 agtgattcat agattttatc aaaaaaacaa tcgttaattt tgcggtatat tctgcaagga
     9181 gacgattgtt ttttaaattt agttcaaatg aacgcgtttc ttttgattca taactaatgt
     9241 aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat attcaaggag gtttcatcaa
     9301 tggaaaaaat caaagttgtc attgtaggtg catctcatgg agggcatcaa tcgatcttgg
     9361 aactattgac acgatacggt gaaaatgtag atatcacgct atttgaagca ggagactata
     9421 tttccttcat gtcatgtggt atggagctgt atttagaaga caaggtaaca gatgtaaatg
     9481 atgtacgtaa tttcagacca gaaaactttc cgcaatccaa tgttcatatt ttaaataatc
     9541 acgaagtaaa aacgatcgat gccgataaaa aaacagtaac ggttactagc gcatcagata
     9601 acgaaacaaa agaatatcct tatgacaaat tgattttgag ttctggtgtc aaaccaaaca
     9661 atctgccagt accaggagca gatctagaaa atgtctactt gatgcgtgga tacaattggg
     9721 caaccaaaat caaagaaaaa ttaaatgatc cggctattaa aaaaatcgct gtcgtaggtg
     9781 cgggttacat tgggatcgaa gcagcagaag caagccgaaa agctgggaaa gaagtcgtat
     9841 tgcttgatgt gatcgataga cctttaggca cttatttaga tgctgagatg acagatattt
     9901 tagaacaaca cctaaatgaa aacggtattg aagttttgac aaatgcaaaa atcgaagctt
     9961 ttactggtaa cggaaaagtt gaggcaatca aaacaagtga aaaagaaatc ccagcagatc
    10021 tagtcattca agctgccggt gtgaaggcaa atacagaatg gttaaaaggg atcgtcgact
    10081 tagatgaacg tggctggatc caaacaaatg aatacttgca aacgaatctt ccagatgtgt
    10141 atgcagtagg agatgctaca cttgcttatt ctatcccagc acgcaaaaaa atgccgatcg
    10201 ctttagcgac tgttgcacgt agagaagcac gttatgtagt aaaacatctt tttgaagaag
    10261 taccaaacaa accgttcggc ggagtcgtag gttcctctgc attaagtgtc tttgactatc
    10321 attttgcagc aagcggattg aatagcttta cagcagcaaa agcaggagta gatgtacgag
    10381 tgtcttatta tgaagatacg ttacgaccaa aatatgtacc agaagaacaa ggaaatccaa
    10441 aagtgtccat ccaattattc tcggatccaa tgacccatca attgctagga ggagcagtat
    10501 tatctaccta tgatgttaca ggtcaaggaa atgtattggc actagcgatc caaaacaaac
    10561 tgactttaga agaactagca gaagctgact tcttcttcca gccaggcttt gaccgacaat
    10621 ggagcatgtt gaatctagcc gctcaacaag cgttaggaga agaaccattt gtagaataag
    10681 aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct tgcatcaagt aataagaaca
    10741 gccaagtaaa aacagcttct tatgttttta acttggctgg acttattacc gcagatgcaa
    10801 gcttttgaac accgtttatt ttcaaaaaag ggactgtgac acgatttgtg taccgacccc
    10861 caaaagttag accaaaaaat ctaacttttg gaggtcggta ttttttatgg ttaaatatac
    10921 ttttgaactt aaattaaaga tcgttcatga ttatttggat ggcaaaggcg attggaccgt
    10981 aaaaatccag atcaagagat tgaagcggaa atgcttaaaa ttcgggagaa gcacaaggat
    11041 tatggctgtt tacggatgac caatgaacta agaaaccgcg gtttctctat caataaaaag
    11101 aaagttcagc gcttgatcaa gaagctagga ataaaagtaa cggcatacac gcgtaaatca
    11161 cgtcgctaca attcttatcg aggaaaagt
//
LOCUS       DAZUMK010000090        11164 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000090 DAZUMK010000000
VERSION     DAZUMK010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.092"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACG808_002110"
     CDS             6..305
                     /locus_tag="ACG808_002110"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5047721.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACG808_002111"
     CDS             378..1739
                     /locus_tag="ACG808_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFS5047722.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACG808_002112"
     CDS             1761..2021
                     /locus_tag="ACG808_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047723.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACG808_002113"
     CDS             2035..2787
                     /locus_tag="ACG808_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFS5047724.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACG808_002114"
     CDS             complement(2881..3486)
                     /locus_tag="ACG808_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5047725.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACG808_002115"
     CDS             complement(3539..4513)
                     /locus_tag="ACG808_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFS5047726.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACG808_002116"
     CDS             complement(4891..5610)
                     /locus_tag="ACG808_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5047727.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG808_002117"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG808_002117"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFS5047728.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACG808_002118"
     CDS             7161..8558
                     /locus_tag="ACG808_002118"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5047729.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDFFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACG808_002119"
     CDS             8639..9079
                     /locus_tag="ACG808_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS5047730.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACG808_002120"
     CDS             9227..9553
                     /locus_tag="ACG808_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS5047731.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACG808_002121"
     CDS             9554..10303
                     /locus_tag="ACG808_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFS5047732.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ACG808_002122"
     CDS             complement(10383..10838)
                     /locus_tag="ACG808_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS5047733.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT         3546 a         1955 c         2293 g         3370 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaaaatta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag atttttttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 atta
//
LOCUS       DAZUMK010000091        10774 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000091 DAZUMK010000000
VERSION     DAZUMK010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.093"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            353..622
                     /gene="rpsN"
                     /locus_tag="ACG808_002123"
     CDS             353..622
                     /gene="rpsN"
                     /locus_tag="ACG808_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFS5047734.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            637..816
                     /gene="rpmF"
                     /locus_tag="ACG808_002124"
     CDS             637..816
                     /gene="rpmF"
                     /locus_tag="ACG808_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFS5047735.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            848..1225
                     /locus_tag="ACG808_002125"
     CDS             848..1225
                     /locus_tag="ACG808_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047736.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1246..1395
                     /gene="rpmG"
                     /locus_tag="ACG808_002126"
     CDS             1246..1395
                     /gene="rpmG"
                     /locus_tag="ACG808_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFS5047737.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1419..2402
                     /locus_tag="ACG808_002127"
     CDS             1419..2402
                     /locus_tag="ACG808_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFS5047738.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2432..2554
                     /locus_tag="ACG808_002128"
     CDS             2432..2554
                     /locus_tag="ACG808_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFS5047739.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2572..4095
                     /locus_tag="ACG808_002129"
     CDS             2572..4095
                     /locus_tag="ACG808_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFS5047740.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            4119..4358
                     /locus_tag="ACG808_002130"
     CDS             4119..4358
                     /locus_tag="ACG808_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5047741.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4551..5012)
                     /locus_tag="ACG808_002131"
     CDS             complement(4551..5012)
                     /locus_tag="ACG808_002131"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS5047742.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5207..5425)
                     /locus_tag="ACG808_002132"
     CDS             complement(5207..5425)
                     /locus_tag="ACG808_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5047743.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5543..7159
                     /locus_tag="ACG808_002133"
     CDS             5543..7159
                     /locus_tag="ACG808_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFS5047744.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7400..8740)
                     /locus_tag="ACG808_002134"
                     /pseudo
     CDS             complement(7400..8740)
                     /locus_tag="ACG808_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9178..9324)
                     /locus_tag="ACG808_002135"
     CDS             complement(9178..9324)
                     /locus_tag="ACG808_002135"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047745.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHIKKEIVVRP
                     LQIY"
     gene            complement(9363..9557)
                     /locus_tag="ACG808_002136"
     CDS             complement(9363..9557)
                     /locus_tag="ACG808_002136"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS5047746.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            complement(9467..9799)
                     /locus_tag="ACG808_002137"
     CDS             complement(9467..9799)
                     /locus_tag="ACG808_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5047747.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(10313..10504)
                     /locus_tag="ACG808_002138"
     CDS             complement(10313..10504)
                     /locus_tag="ACG808_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5047748.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(10598..>10774)
                     /locus_tag="ACG808_002139"
     CDS             complement(10598..>10774)
                     /locus_tag="ACG808_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS5047749.1"
                     /translation="IKLAKILPLLKEPEQIFLIEKFIFDKTDKEIGKVLGITRQGATN
                     LKHRLYKKIQKMLV"
BASE COUNT         3581 a         1704 c         2095 g         3394 t
ORIGIN      
        1 aaaaaattgg cgataaacca gttaaacata aaagattgaa taaaaagtta tagtttcttt
       61 catattgccg ttttaagtag aggaaaagct acttaaaacg gtatttgtgt ttgattggac
      121 taataaaagt gaattcttat ttttcagagc ctaaactgca tattctttta aatgatttga
      181 ttatctctat gtaccgataa attcgttttc aaatagttat ctattcatat gagttttatt
      241 tcatgttatt tattgttgac aagagcaact cttgttggta aagtatcagt gtcaaaacgt
      301 aacgattacg atttgaggtg tctaaatcaa aataaaagga gtataaaggt atatggcaaa
      361 aaagtcgaaa attgctaaag caaagaaaca aatggcgatg attgaaaagt atgcagataa
      421 acgtcaggaa ttaaaagccg caggagatcg caccgctctc gctaaattgc caagagattc
      481 aaatccgaat cgtctgaggc ttcgtgatca gacggatggt cgtccaagag ggtatatgcg
      541 aaagttcggt atgtcccgta ttaagtttag agaacttgcc catcaagggt tgattcctgg
      601 agtaaaaaaa gcaagttggt aaaaggagga aatatcatgg cagtaccagc aagaaaaaca
      661 tctaaggcga agaaacggtt aagaagaact catcaaactg tagtaaaacc agaaatttca
      721 tttgacgaag cgaatggtga ttatagacgt agccatcatg tatctttaaa agggtactat
      781 aatggaaaga aagtaattaa aggagcttca aaatagttta atatataact tttaggaggg
      841 aatcaaaatg gaaattattt acccaccttt agttgaagaa ggattaaagt attatcttga
      901 aacgacccag caatcgctag ataaatgcac tttttatcgt tcgatggtag agagaggaat
      961 tattactgaa acgggtttac cgacccaaca ggcaattgaa aatggattag tcaaagatta
     1021 ctatgaggac caaggcttat catttgatga ttttttaaga atctatccaa tttttgaaga
     1081 gtatgacgag gagttatttc aatgtatcga tggttattgg gagatacctg ttgatatgaa
     1141 agaaaattta gtttcacaat tggaatctgg cgagttaact tttgaagatg cacaacaaat
     1201 tcaagcatat cttgaagatc gataagtatt gaaggaggaa gatttatgcg tcaaaatatt
     1261 attttggaat gtgtcgaaac tggagaacgt ctgtatctca ccagcaaaaa taaacggaat
     1321 aaccctgaac ggttagaatt aaaaaagtat tcgccaaaat tgcgcagacg ggcaattttt
     1381 aaagaagtga aatagctaag tggtaaaaag gtgacaaaat gggcatacca gttacaatcg
     1441 tttctgggtt tttaggggct ggaaaaacaa cgctaatcaa tcaagccttg caagtctcac
     1501 cctaccccag ggaagaaatt attataattg aaaatgagtt tggtgaagtt gggattgatc
     1561 acaaattatt agttcatagc gaggaacggg tgttacaact aaataatgga tgtatgtgtt
     1621 gcagtcttcg cagtgattta ttagcaatat tgatttcttt actggaaaca atagaagaac
     1681 atcaacaacc aatttcacaa atcatcattg aaactacagg tatagcagat ccccaaccaa
     1741 tcattcaaac acttttaaca gcgcctcaac taagaggaag agtatatatc gatagtttat
     1801 tgactgttat tgatagtgac agttttgaac ttattgaaag agaaccacaa gcccttaagc
     1861 aacttgtcat ggcagatcgc atttttgtgt ctagaaaaca aaacttgaat ttatctgcac
     1921 taaaaaaatt acagagagaa atcaaaagta ttaacccttt atccgatatt cgaatgttca
     1981 cacatagtga aacgattcaa aaaaaggatt tttataattt gaaaaaattt aatcatccct
     2041 tagtttttga aagtgattca tcagaaaatc actataatgg caaatactat catcaacacc
     2101 atcaagtaca tgagtttaaa tcaattctct taacttcaga tcaagacata aaaaaagatt
     2161 ttttactcca atggattgac tggctgattt taaccaacca agggagcctg tatcgtttta
     2221 aaggattgat tgctttacaa gagcaggatt ttatagttgc catgcaagag gtaaacgaac
     2281 aggttaattt tcaatacacc actcgacaaa aagaccaaac cactattatt ctaatcggaa
     2341 aaatgttgga tgaagaaagg atcaaacaga gttttgataa attactggcg gaaagcaaat
     2401 aatgataagt agtgattggg ggtaatttct aatggagaaa acggatatat ctagtgcata
     2461 tcgacgactt aaaagcccga atattaaaac tagaaaaagg gcattaaaaa ttatcaaaga
     2521 acataaacga aataaaatga agaaattagc ataattaagg aaggaaaaag aatgaagaaa
     2581 atcagtgtag gacttattgg gatagcagct ctaggattat tgggagcttg ctccagcact
     2641 aatgatgcaa aagtatcgaa tgataaagat ggaaaattag aaattgtaac aacattctat
     2701 ccaatgtatg atttcaccaa aaacatcgtc ggcgatgaag cgaacgtgga tttaatggtt
     2761 ccagcgggat cagaacctca tgactatgag ccctcagcta aggatatggc aaaagctcat
     2821 gatgctgatg tttttgtata tcataacgaa aacatggaat catgggtacc aaaagctaaa
     2881 gaaagttgga agaaagctgg gcctaatgta gtagaaggaa caaaagacat gattttattg
     2941 ccaggtagtg aagaagaaga tcatgatcac ggagaagaag atcaccatca tgaactagat
     3001 cctcatacgt gggtttcacc caaaatggca attaaggaag tttcaaatat caaggatcag
     3061 ttagttaaat tgtatccgaa aaaagcaaaa gtatttgaga caaatgctga aaagtatttg
     3121 actaaactga aacggttgga tgctgactat accactagtc ttaaagaagc gaaacaaaaa
     3181 agttttgtga cgcaacacgc agcctttgga tatttagctt tggattatgg attgaaacaa
     3241 gttcctattg ctggactaag tcctgaagaa gagccatctt ctggtcgatt agctgaatta
     3301 aaagagtatg ttaaaaaaaa taagatcaat tatatttact ttgaaaagaa tgctaatgat
     3361 aaaattgcaa aaactttggc caacgaagct gggataaaat tggaagtact gaatcctctt
     3421 gaaagtctta caaaagaaca aatggataat ggtgaagatt atgtttcagt aatggaagac
     3481 aatctaaaag cgttagaaaa aacaactatg gttgctggtg aagaggtagt tccagaaaaa
     3541 gaagcgaagg atgaaaagac agttgctaat gggtacttta aagatgccga tgtgaaggat
     3601 cgcgaattga gtgattatac aggcgaatgg caatctgttt atccgttgtt aaaagatgga
     3661 attttggatg aagttttcga ttacaaagca aaaatcaata aagacatgac agctgccgag
     3721 tacaaagatt attatactac tggctataag acagatattg atactatcaa cattaaagac
     3781 aacaccattg attttgtagt caacggtgaa catcatcaat acacatacaa atatgttgga
     3841 tacaaaattt tgaattatga aaaaggaaac cgtggtgttc gttttaattt tgaaactgat
     3901 gatgcaggtg ctggtcgttt taaatatatt caatttagtg atcatggaat tgcaccaagt
     3961 aaggcagagc atttccatat tttctttggt ggagaaagtc aagaaaaact ctataatgaa
     4021 ttgcataatt ggcctacttt ctatccagct tcattgtcta aacatgaaat tgctcaagaa
     4081 atgatggctc attaaaattc aatttttgga ggaaatacat ggtaagaaaa gtattattgt
     4141 ctatggattc caagaatatt gatgcttatc ggcgttatcg atttgcaaag caaatgagat
     4201 taaatgggag tacattagct gatattgctg caactttaca tgttagtatt gacacagttc
     4261 atagagtatt aaaacgggat cctgattgtt gttttatcaa ggtcaatact ggaagaagta
     4321 gacgtttagc aaaacggcat aatagcttgg ccaattaatt gataatacca gagtagatca
     4381 tttttccctt atttggattt gatctgcttt ttattatgtc ttgaattgtc aaatcaagcg
     4441 caacacttta tcaaagaaga cttcataagg tgttctccaa tctaaacact ttctaggtct
     4501 gttattcagt ttttcaatca tctgatttat cgttgagtta tctaccgtag ctatctcttt
     4561 cgattttggc aagtattctc gaatgagtct attcgtattt taatttgttc attgttgcca
     4621 agggcatgag gatctggaaa ataaaagggc atattattca atgcttctgt aactgcgaaa
     4681 tgattggaga attcttttcc tctatctgga gtgatcgttt tgaccgcctc atttggaata
     4741 ctgcttaaaa gttcaatcat tttctcgctc acataagcgg ctttctttct ggggaccttc
     4801 tttcctgcta aaaggaagca tgattttctg tctgtcaaag tcactaaaca agtaccacca
     4861 gttttgccag ctacaatatc ggctttccaa tgaccaaaat cacttctatc atctgcggat
     4921 tttggacggc catggatcga atgagtaagg caaatctttc cacgagactc gacatgattt
     4981 tttgtatgtc ttgtttttcc tcgatggtgc aatgaacgga tcaggccttt attgccgtgt
     5041 gagaggggtt ctgtatcgaa aagatcattg caaattgcac gttagatcgt gataaaacta
     5101 attgaaaagt ccatatgttc atgcttcaaa cgattcgaga tttcctctgg cgaccactga
     5161 aaatcgacaa ataaatgacg aacaattgac caaactcacg gatcatttag aatgtgataa
     5221 cgtccgcatt tttttcttcg ataagcataa tttttttccg ctttggaagg tgaatagttt
     5281 ttttcttgtt tcttctcagt tcatgtaaaa tagtaaacgg gctatgctga atgtcgttgc
     5341 caattatact aatagaaaat ccttcttcgt gaagcagaaa gattctttct cgttcgagta
     5401 tagtaagatg acgatactgg ctcataatta ttttccttct aggagtgttc tcgacaaact
     5461 tatactagtt ggatatcgat tatgactcat ttttatgtgt tgcacttaaa gtgtaaattc
     5521 aaggtataga aagaggttcc ctatgaaaaa aatacctatt acaatcttaa ctggctatct
     5581 tggttcagga aaaacgatac ttctaaatca tattctgtac ggtgatcacg gaaaggaaat
     5641 tgctgtcatt gtaaatgaca taggggaagt aaatgttgat ggggtattag tcgacaagaa
     5701 aggttttaaa agaactggag aaaaacttgt ttcgatgccg aatggatgca tttgttgtac
     5761 attacgcgaa gatttgattg ttgaactaga tcaattagtg caattttctg acttgaatca
     5821 aattgttatt gaagcttcag gaatcagcaa accaattcca attattcaag caattatatt
     5881 agctaaaaac cctttagaca ttgatttgac ccaacgatta gagattggtg ccattattat
     5941 agtagtggat gtaaaacgtt tttgggataa tttcgaaacg ggtgaaaaga ttcaggaaag
     6001 aaaagctgaa ggtaatgtag ttgaagaaga acaggatatt cgtggcttgt taattgatca
     6061 attcgagtgt tgtaacatat tacttttaaa taatagtgac ttagtgtcaa ccaagacgat
     6121 ttacaaaatt aacggtctaa ttgctaagtt acagtccaat gccaaaataa ttaggacaat
     6181 taaaggtgaa gtagacttat cgaaaattct aaatacaaat ctttttaata tagatgagac
     6241 ttcctattca gtgggctggt tcaaagagtt agagcttggc tatcaaaatc atattcctga
     6301 aacagaagaa tatggtatct caagttttat ctatcgaaat cggttactcc ttgacgcaca
     6361 aaaatttgcc catcttacgg ataaaaattt tcctggcaat attacaagag caaaagggtt
     6421 tctttggttt gcccaacata gtgatttttc gacgatcttt gaaacatcgg gtagctataa
     6481 aagtattgaa tcattaaatt attgggtagc tagtttacct aaaattgagt gtcaaatgat
     6541 tatacaaaaa aatcccgaat tgaaggaagc ctggcatccg ttgtttggtg aacgcattaa
     6601 taaactagtt tttattggta tgaagatgga tcaacaatgt attaaggaac aacttgaggg
     6661 ttgtttaact gattctctta atgcagataa tttccgaaaa atttcaccat ttccatggaa
     6721 aaagtctaat atgtttagcc agatatcgtt aatgaaattt ttaagaaaag ctggtgtcaa
     6781 gataggcgat gtcataatta ttggcatttt gattattacc tcttttattc ccttatttgt
     6841 attctctata gaaaaggaga atgaaacaac tgaagatgct atcaaatatg ccatcgttcg
     6901 aattgatggc aaggaaatgg atcgcttcga tttggataag atcaatcata aactcgtgaa
     6961 ttatcatcct gcgaagggac aatataatat tgttgagatt aaagatggaa gaattcgagt
     7021 taaagaagat aatagtcctg atcaaatcgc ggttagaact ggatggatct caaaacccgg
     7081 tcaaacgagt atttgtttac cccataagtt agttattagt attgagaaaa aagaaagcac
     7141 agattactac atttactaat ctggaaatag atgaggaggt ttttaataag aaaaagatat
     7201 tattattaat aatagcaggg gctttattaa gtgtgttaag cgcttgtgga caagaaaaag
     7261 aagcggtgac aacaggcaca agtatcttta acgggacctg caatggttta gttaacagtc
     7321 ggttagatta cttattttgg ctcttcgtca atgagtgttg gtgaagaaat tcaatagaat
     7381 aatcgagata ctcacacggc tagacagcta tcttgtctag ccgtgttgtt gttctggctt
     7441 tagtcagttg ggtagtattc ttagttttct taaaatattt tcctcgatat aaaaagattc
     7501 gttctcggta gttttgaaag tttctatagc cataggcaat tcgttttagt actttgatat
     7561 ggttattcaa acattctata gggccgttgg aatagggaac gcgcaacgct cgtttgattt
     7621 ctgtttggta tttcttgaag gtcgtgaaca ctggttggta gtgctctggc aactccttgt
     7681 agtcttcttt taggagcgcg acgaattcag gaacatcttg tcttctgata acggaaagaa
     7741 aatcctggta gacttcgtag cctcgcttta aggaatcatc ataagataat agccgatcga
     7801 ctatctctgc ttctgtcaga tgatctcgaa atgacggacg ccagtagcga tgctgacctt
     7861 tcaattccca cgcattcttt tgaagtagtt tccagtattt ctttaagtgc ttcgaccgac
     7921 gatccccttt tttaaaggtc ttcattactt gaattcggtg gttggtaaaa gcccgaccaa
     7981 tgtgttggct aatatgaaag cgatcggcga cgattcgggc attcggaaaa cactctttca
     8041 ccaatgtgcg atagggtgta tagaaatctg aaaccactag acgcacttgt tcacggactt
     8101 ttatagggta gcgcaagaaa tagtcgcgta aaggattgag ttgccgattt tccacaatat
     8161 caatcagttg atgggtcttg ccgtccatca tgacaaagct catggctccc gagacatttt
     8221 tcactgattt gaattcatcg aaacacacca cttctggtaa cagctggctc aaagaaatgg
     8281 ttttaggccg aaggctgcga agtattcgaa tgacagtggt cggagaaatg tgcttcatac
     8341 gtgcgatcaa agacatagaa agaggttcgc ttaatagctc aagaatggct tgtttgactc
     8401 tgcgagcaat ggagcagcga cgatcactaa ctgaatcttc tgctaaaaaa gtctttccac
     8461 agtgtttaca tttgaagcgc tgtttgttaa tccgcagaat ggtcttgtag ttactgacat
     8521 cattcaataa gatggatact tttttccagc cctatttaat gatcgtttgt tcattttgat
     8581 agtggcgatg aaagcattcc tccggttgat aggtcaatgt gccatcaaaa gcaaaacatg
     8641 tttcgtcatt gatgcgctct ttgcgaaagg catcctcatg aaaggtaaga tttaggtcta
     8701 gaatatctag taactctttg gtaaactctg aataggacat cttgaaacac tactttgaat
     8761 ggtttttgtc gactttattc taaaggattt taaggtgtct gtttgcgttt attagaaaaa
     8821 agaaaaagta ttggcaacag aaggcgtcac caacactaga tattatagat cccttatttt
     8881 cttcgcttca aattgtcaag gagtcatcat aaattttgta tggattcttt tgtttgggta
     8941 gtagtcttct aaataatctt ggattaacac cttcatctct attttacttt tatatttgtt
     9001 ggagtcgatt aattctcatt tgattgatcg atgaaacgct tcaattactg cattgtcaac
     9061 aggagtccca gtttggctca ctgaatgtgt gatgtggtgg tcgttcaaca gctcttgata
     9121 aataaaagac cgatgctggc tacctctatc ggaatgaatt accgtaggca attctcctta
     9181 ataaatttgt aatggacgga cgacaatctc tttttttatg tgtgaatcta ccatatagta
     9241 acgtagaata cgagttgcta agtcaatata ggtggacaaa tagaggcgac cgtcattaca
     9301 tggaatgtaa gtaatatcca tcacccaaac agcatcaatt tggttctgtt caaattatcg
     9361 attcaacatt tttttaccat ttctttgatt gttttttctc taccatgaga agatttgcgt
     9421 cgatgaaatc ctcttgtgca taaattcatt tcacgcataa gaacagctac aacacgttta
     9481 ttgatttcga ttccttcatc gtaaagtttt tgagtgatcc ctggagatcc gtatcgatct
     9541 ttgtgttcgt agaacactcg tcttcctctt ttagtcgttt attttcctgt tcaagaatgt
     9601 agaaatctgc tttatcaggt aatctttttc cttgtccagc aaaagcattt ttcccgtatc
     9661 gacgatattc tgaaacccat cgggaaattg ttggacgaga gatattgtat tcttcagcaa
     9721 ttttcttggt agttactttt ttctcaagga tatcctgtga aatctttagt ttaaatttac
     9781 gatcatatgt ttttctcaat agagttcatt ccttccggta acgtaactga tagtcaagaa
     9841 aagtgtatca tagcccaact tagtatcagg gtttgtcaac taaactgtgg aagttgatag
     9901 ataagagttt ttaacaacca aaattctctt gactattttt gaaatcagac aaattttgat
     9961 tggacacata gttgaataaa tctaccgtcg ttacggaagg taaatcgcat gcttttcaat
    10021 tctagaggag aagtcctcgt tgaagtacca tagcccagtt ctggattcga cctcctgccc
    10081 acttcatacg taaataaagt aattttagga gtgagactgt gacaaaagtt atttttaagc
    10141 taaaaacgaa ttaaaaaacg ttgataatta aaatcattcg ttttagattc tatgaatcat
    10201 tatagaacgc tgtaaaatcg atactttata attataaaga agatagtttc aaatttattg
    10261 ttcgaaattt gaaactatct ttttacttat gtcacagact cccttagttg agttattctt
    10321 taatttcgaa tttcgagata gctagatata ttgcaagcat acattcttga taacaatctt
    10381 gatcatattt tccagttctc caggattgct tatgaatcaa agagctaaat cttttgagaa
    10441 ttgcctcaac agcttcttca tttccagcct ttgctaaaat gaaatcttct tttaaagaat
    10501 tcataaatct accttcttcc catggactta taatctattt ctttgggaag gaggagaacg
    10561 agcaaagaag catacaaaat tagttattat gcagatatta aaccagcatt ttttgaattt
    10621 ttttgtaaag tcgatgtttt aaatttgtcg caccctgtct tgtaatcccc agaacctttc
    10681 caatttcttt atcagtttta tcaaagataa atttttcgat aagaaaaatt tgttcaggtt
    10741 ctttcaacaa aggaaggatt tttgctaatt ttat
//
LOCUS       DAZUMK010000092        10497 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000092 DAZUMK010000000
VERSION     DAZUMK010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.094"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..461)
                     /locus_tag="ACG808_002140"
                     /pseudo
     CDS             complement(1..461)
                     /locus_tag="ACG808_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
     gene            complement(713..1072)
                     /gene="rplT"
                     /locus_tag="ACG808_002141"
     CDS             complement(713..1072)
                     /gene="rplT"
                     /locus_tag="ACG808_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFS5047750.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(1125..1325)
                     /gene="rpmI"
                     /locus_tag="ACG808_002142"
     CDS             complement(1125..1325)
                     /gene="rpmI"
                     /locus_tag="ACG808_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFS5047751.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(1397..1921)
                     /gene="infC"
                     /locus_tag="ACG808_002143"
     CDS             complement(1397..1921)
                     /gene="infC"
                     /locus_tag="ACG808_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFS5047752.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(1965..2090)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            2241..2873
                     /locus_tag="ACG808_002144"
     CDS             2241..2873
                     /locus_tag="ACG808_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFS5047753.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2875..3780)
                     /locus_tag="ACG808_002145"
     CDS             complement(2875..3780)
                     /locus_tag="ACG808_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFS5047754.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(3865..4524)
                     /locus_tag="ACG808_002146"
     CDS             complement(3865..4524)
                     /locus_tag="ACG808_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFS5047755.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     CVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVS
                     I"
     gene            4840..5784
                     /gene="mvk"
                     /locus_tag="ACG808_002147"
     CDS             4840..5784
                     /gene="mvk"
                     /locus_tag="ACG808_002147"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFS5047756.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5777..6754
                     /gene="mvaD"
                     /locus_tag="ACG808_002148"
     CDS             5777..6754
                     /gene="mvaD"
                     /locus_tag="ACG808_002148"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFS5047757.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6767..7852
                     /locus_tag="ACG808_002149"
     CDS             6767..7852
                     /locus_tag="ACG808_002149"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFS5047758.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7849..8913
                     /gene="fni"
                     /locus_tag="ACG808_002150"
     CDS             7849..8913
                     /gene="fni"
                     /locus_tag="ACG808_002150"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFS5047759.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(9162..9965)
                     /locus_tag="ACG808_002151"
     CDS             complement(9162..9965)
                     /locus_tag="ACG808_002151"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFS5047760.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9985..10308)
                     /locus_tag="ACG808_002152"
                     /pseudo
     CDS             complement(9985..10308)
                     /locus_tag="ACG808_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
     gene            complement(10341..10478)
                     /locus_tag="ACG808_002153"
                     /pseudo
     CDS             complement(10341..10478)
                     /locus_tag="ACG808_002153"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
BASE COUNT         3107 a         2220 c         2008 g         3162 t
ORIGIN      
        1 aaatacctaa tgacatcaag atcggcaaca caataactgc aatagaaatc actggtccga
       61 ttcctgtcat cgacatgaat ataacagcgg ttacaataca tagtaagctc atcgtgattc
      121 ttggcttgtc ccctccaagt tcaactacct ttcgaatcag ggtagctgcg attccgctat
      181 ctactagaac acgcccgaaa aaggcaccaa agaagacatt taccaagatc gatttggcgt
      241 actcagctgg ccccgtcgta tagatatgcg tcaacacatc aattactcct tgcccttcca
      301 tcgccgagtt tggtgaaaac gtgtttccga ctagcgctaa tcccatccat acagtagcca
      361 tgatgacaaa agccgaccat caagttgtag ccttttacac agtaccagac catgaaaaag
      421 aatgagataa ctaacaaaat accaataata atatcactca tgttattcct ccgtccattt
      481 tgtttttctg ataagcaaac taatatatca gtcaaactca ttctaactta tgtagttagc
      541 gctttcatta aaggatgacc ataaatgttc agttttcaat taaaaaacct taattatgtt
      601 attttagact tatacgttat tgtcctgttt catttgatgg aggattgtaa ataaaaaaag
      661 aggaaagaac ataacttttc gttatcttct ttcctcttta taattgttta gcttatttag
      721 ccaaagcttc ttttgcttgg tcagccaatg cagtaaatgc tgctgcatcg ttaacagcca
      781 agtcagctaa cattttacgg ttgatgtcga tttcagcaac tttcaaaccg tgcattaatt
      841 ttgagtagct caagccattc atacgagctg ctgcgttgat acgagcaatc cacaatttac
      901 ggaagtcgcg tttcttttga cgacgatcgc gatatgcgta gttgtatgaa ttcattactt
      961 gttcttttgc tgttttaaat aaagtgtgtt ttgaaccgta gtaacctttt gctaatttta
     1021 gcactctttt acgacgttta cgagttactg ttccaccttt aacacgtgcc atgtataatt
     1081 cctcctaata aaaataaatc ttcagattct gttatttgta cttcttattt catttttgct
     1141 agttgttggc gaatacgttt gaagtcgcca ctagaaacca tgcttgcttt acgcaattga
     1201 cggcgttgtt tttttgtttt gccgtggaaa cggtgacttg taaacgcgcg gaagcgtttc
     1261 aatccgcctt taccagtacg tttgaaacgt tttgctgatc cgcggtgtgt tttttgtttt
     1321 ggcatgacta tatttcctcc tcaaaatctt tcgttatccg actatcggct gtccaacagc
     1381 cagttagtca ttcaaattac ttactgtcgt ttttcggtgc cagcgtcaag aacatgctgc
     1441 gtccgtccat tttcgctttt tgttccactg ttgcaatatc cgcagtttct tcagctaagc
     1501 gattcaagac tttttgacca atttctttat gggtaatggc acggcctttg aaacggatag
     1561 aagctttcac tttatcgccc ttttccaaga acttacgtgc attacgaagt tttgtattga
     1621 agtcattcaa gtcgatcgta ggacttaagc gaacttcttt tacattgatc actttttgtt
     1681 ttttacgcgc ttcgcggtct ttcttttgtt gttcgaaacg gtatttaccg tagtccatga
     1741 ttcgcgcaac tggtggtttc gcaccagggg caactaacac aagatccaag tttgcttgtt
     1801 ctgcaatatt caatgcttct gcttttgtct taactcctaa ttgctcacca tctgaaccga
     1861 tcaatcgtaa ctctcgtgca cgaatgccgt cgttaaccat catatccttt gctatggtca
     1921 ttcacctcca aatattatga gagaaagaaa agcttaccga ataaacggca ataaaaaaac
     1981 gagttctctt acagaacccg cgcgtacttc acctaataga accagtgaaa actatctagc
     2041 ccagtgacat tatcaactaa ggcgagaagc gggtgcttct gcttttattt ctcaaccatt
     2101 atagtatagc aaaaactaat ctttttgtca atgtttttca tacagtttgt cttccttttc
     2161 tgaaaatgat tagattagca ggctttctag cagtgattcc cgcttctttg ctatactaaa
     2221 gaaaaacgag gtgaacccac atgaaaaaaa tcgaacctat tccaccttct cttcctcttc
     2281 ttacaaaggg agagttttat ttagaagatg aggagatctt ctctggtctt ctgatcaacg
     2341 gaggtaacca aagctatctc gatgccaaaa atatggtatt aagagagagt catatccaaa
     2401 aacttacgat gcaaaaaaca ggtttacgtc attttgaatg cagtaatgtc atttttgaaa
     2461 aatgtgattt ttccaatctg gagtggattg gtggcagttt ccaccaagtc gtgtttcatc
     2521 aatgtaaatt aacaggtacg aattttgccg aaagttatct tcgagactgt actttcactg
     2581 attgtattgc ttcgatggct tcctttagta gtacaaactt gaaagctgtc tccttcgatc
     2641 attgtcaatt agaagacagc gaattttatg aggtcacttg gaaaaacctt tttttgtctg
     2701 acaatcagtt aaccggttcg aactggtttc gaacttctct gaagggatta gactttacga
     2761 ctaatttctt cacaacaatc gctttatctc aagattcttt ggcgggacta atcgtcaatc
     2821 aagagcaagc acttgtgatc gcccaagccc ttggtttgca gatcaaagac tagatcaacg
     2881 aatccgccgc cacagtgcaa taagcatcca aatagataag accaaactga tgatcaaggc
     2941 gatttcccat ccgaaaacgg aatgggtaaa gggtaactgg agattcattc cgaagaatcc
     3001 tgtcacaata gttggaacgg tcaagatcag cgaccagacc gtcaaaaatt tcatcgtgtc
     3061 attcaagttg ttattcagca aattattata agtgccagag agctggtcta aaatctgcga
     3121 agctaagttc gtcatttcaa ctgcttgtcg tgcttcgatc agtgcatcgt ccaactcttc
     3181 tttttccttt tctgacaact tgcgataaat agccagtgcc ttgatttgtt ctaaaagtac
     3241 tacattttgt tttgttcctg taactaagta aaccaaaccg atctctaaat ctgaaagttg
     3301 cagcagattc ttgttcgttg ttttttctct taatttcaga ttaagccgct gacgttcact
     3361 attcacttcc tcgattaaag gaaaaaagga atcagaaatc aaaaacaatg tacgaaacaa
     3421 taaactgtat acactcatgt ctggcatccg ttccaaaagc gatcgaatta gtcgaatcac
     3481 gtactcggta ttcggcgtgg taaatgtaaa caaaccgtct tcttttaaaa taaaagcgat
     3541 aggactagtt tcataatgat tatcgacttt ttgctggtgc ggaacatttg atacaagcag
     3601 taacgcttca tcaaaagaat cgtactctac gcgcgcccgt tcatttttat ctagagaata
     3661 agtcagcatc tcgtcactaa tgccatattc ttcttttaag tttttaacag ctagcacatc
     3721 gttttgctgt acattgatcc aaaaattacg gtcttgatta aaatagtgtt tttcccgcat
     3781 actctatcct cctgacattt gtttccagta taaagaaaaa agtcgctcaa ataaaggttt
     3841 accctttatt tgagcaactg taaatcaaat ggatactttg gtttggcgaa tctctgctaa
     3901 tttttcttcg atcattttca agacttcttc ttttgcttga ggatcttcca caaaattata
     3961 gcggtctcca tcgatttgga tttttggact ttctttgtat tcttcatacc attgatcata
     4021 acgtctgttc aattctttgt aataatcgta tagacttggg tcaaaggaca attgttcata
     4081 cgaacgacca cgtttttcga tacgctctaa catcgtatca aaagatacac gaatatgaac
     4141 taataaatcc ggatgttttt tatgggcagc atagggaagt tcttccatca tgttttccag
     4201 cagttcatca tacacacaaa cttctgtttc tgttgctctt cctagatctg catttagatg
     4261 gaaaagcagt gaatcttcat aaatcgaacg gtccaataca ttattgtcat ctttcattgc
     4321 ttgtttgatg cttgcaaaac gtttattcaa aaaataaatt tgcagcaaaa aagcatactg
     4381 ttcagggttt gcataaaata agggcaagac ttcattatca tcaatcgatt cataaaatgc
     4441 ctgactatca aagtgttcag ccatcatttc tgttaaactt gattttccgg caccaattgt
     4501 acctgctaaa acgatcacgc tcatttacct ccagctcact tcatcactaa atattgtgtt
     4561 taactccaac cttttttaaa tataccccca atatctagta gtgtcaattg tattttttct
     4621 agtcaaagaa gaaaaaaagt caaaaaattc ttaagaaaaa agtgtttttt ctttagaaaa
     4681 cgtattctta gttttacttc tgagatggtt ttagtaaaat aaaggaggaa tgttttttga
     4741 gacccttagt ggacaaaaaa attcgtgaag cagcgcaact cgttttgggc gctttatttt
     4801 ttgcttaaaa atcactttta cgtgagaagg agttagatta tggcaaacta tggccaagga
     4861 gaatcaagcg gaaagatcat attgatgggc gagcacgcgg ttgtttatgg agaaccagcg
     4921 attgcctttc ctttctatgc aacaaaagtc accgcattcc ttgaagagct ggatgcaatg
     4981 gacgatcaac tggtttcttc ctactattca ggaaatttag ccgaagctcc tcatgcatta
     5041 aaaaatatca aaaaattatt cattcactta aaaaaacagc atgacatcca aaaaaacttg
     5101 caactgacca ttgaaagcac gattcctgct gaacgtggaa tgggatcaag cgctgcagtc
     5161 gccacagcag tcactcgtgc tttttatgat tacttagcat ttcctttgtc tcgtgaaata
     5221 ctattagaaa atgtccagct ttcggaaaaa atcgcccacg gtaatcctag tggaatcgat
     5281 gcagccgcta ctagcagctt gcagccgatt tattttacaa aagggcatcc ttttgactac
     5341 ttttctttga acatcgatgc ttttttgatt gtcgctgata caggaatcaa aggacaaaca
     5401 agagaagccg tcaaagatgt cgctcacctc tttgaaaccc agcctcatga aactggacaa
     5461 atgatccaaa aattaggata cttgacgaag caagcaaaac aagcaatcat tgagaattca
     5521 ccagaaacgt tggcacagac aatggatgaa tcacagtcac ttctggaaaa gctgacaatc
     5581 agcaatgatt ttcttaatct attgatccaa acagcaaaag ataccggggc attgggtgcc
     5641 aaattaactg gcggtggacg cggtggctgt atgattgcat tagcacaaac aaaaacaaaa
     5701 gcccaagaaa tcagtcaagc acttgaagat gcgggtgctg ctgaaacttg gatacaagga
     5761 ttaggagtac atacctatgt ttaaaggcaa agcacgcgca tataccaaca ttgctctaat
     5821 caaatattgg ggtaagaaaa atgaagaact catccttcca atgaacaaca gcctttcgtt
     5881 aacattggat gcattttata cagagacaga agtcatcttt tctgatagct atatggtaga
     5941 tgaattttat ctagatggca ccttacaaga cgaaaaagca acaaaaaaag tcagtcagtt
     6001 tcttgacctt tttcgtaaag aagccggcct ctcactaaaa gcttcagtaa tcagtcagaa
     6061 tttcgttcct accgcagctg gattagcctc ctctgccagc gggctagctg ctttagcagg
     6121 agcttgcaat actgctctta agcttggatt agacgatctc tctctttcaa gatttgctcg
     6181 acgcgggtct ggttcagctt gccgaagtat tttcggtggt ttcgttgaat gggaaaaagg
     6241 ccatgacgac ttaagttctt acgctaagcc agtcccttcc gattctttcg aagacgattt
     6301 agcaatggtt ttcgttttga tcaacgacca gaaaaaagaa gtgtccagca gaaatgggat
     6361 gcgtcgaaca gtcgaaacat ccagttttta tcaaggctgg ttagattccg ttgaagggga
     6421 tctatatcaa ttgaaacaag caatcaaaac aaaagatttc caacttctcg gagaaacgat
     6481 ggaaagaaac ggactaaaaa tgcatggaac gactttagct gcccagcctc cattcactta
     6541 ctggtctcct gacagcttaa aagcgatgga tgcggtgcgg caattgagaa aaaaaggcat
     6601 tccatgctac tttacgatgg atgctggccc taatgtcaaa gttttagtgg aaaacagcca
     6661 tttatctgag gtacaagaaa catttaccaa attatttagc aaagaacaag taattactgc
     6721 ccatgctggt ccaggaattg cgattattga ataaagggag atttttatga ttgaagtatc
     6781 tgcaccaggc aaactctata ttgccggaga atatgcagtt gttgaaacag gacatccagc
     6841 agttatcgct gcagtcgatc aattcgtaac agtcactgta gaatccgcac gaaaagtcgg
     6901 aagtatccaa tctgcacaat atagtgggat gcctgtacgt tggacaagac gcaacggaga
     6961 attagtttta gatattcggg aaaatccttt tcattatatc cttgctgcta ttcgtttgac
     7021 tgaaaagtat gcacaagaaa aaaacattct tttatccttt tatgatctga aagtaacaag
     7081 tgagttagac agttcaaatg gccggaaata tggattgggt tctagcggtg ccgtgaccgt
     7141 tgcaacggtc aaagccttga atgtttttta cgcgttgaat ttatctcaat tggagatttt
     7201 caagattgca gcactagcca atttagcagt tcaagataat ggttcctgcg gcgacatcgc
     7261 tgctagctgt tatggtggct ggatcgcttt ctcaaccttc gaccatcctt ggctccaaga
     7321 acaagaaact cagcattcta tcagtgagtt acttgccctg gattggccag gtctatccat
     7381 tgagccattg attgctcctg aagatttacg tttattgatt ggttggacgg gtagccctgc
     7441 ctctacttct gatttggtcg atcaagttca ccgttcgaga gaagacaaaa tggtggctta
     7501 tcagcttttc ttaaaaaaca gtacagaatg tgtcaacgaa atgatcaaag ggtttaaaga
     7561 aaataatgta acgttgatcc aacagatgat tcgaaaaaac cgacaattac tgcatgattt
     7621 atctgcaatc actggggtcg tcatcgaaac gcctgctttg aacaaattgt gtaatttagc
     7681 tgaacagtat gaaggagccg caaaatcttc tggtgcaggt ggtggcgatt gcggaatcgt
     7741 aattgttgac cagaaatctg gcatccttcc tttaatgagt gcatgggaaa aagcagaaat
     7801 cactccactg ccgttacatg tctatagcga tcaaagaaag gaaaaccgat gaatcgaaaa
     7861 gacgaacatg tttcactagc taaagccttt catgataaac aaaaaaacga atttgacttc
     7921 gtacggatca tccataaccc tttgccgcaa atagcagttt ctgatgtcga tctaagtaca
     7981 caggcagttg ggtttacact aagcagccca ttttatatca atgcgatgac tggcggaagc
     8041 gaaaaaacaa aaaaaatcaa ccaagatctt gcaatcgtcg caagagaagc agatttgatg
     8101 attgccactg gttcagtcag cgctgccttg aaagaccctt ctttagccga tacttatacg
     8161 attatgcgac aagaatatcc tcacgggaaa atcattgcca acatcggtgc agggacttct
     8221 gtcgaaagag cccaagaagc tatccgattg tttcacgcag atgccttaca gattcatttg
     8281 aatgcacctc aagagttggt tatgcctgag ggagaccgtg attttaccaa ttggaaagta
     8341 cttattcaag agactcaaac agccattgac gtccccctca tcgtaaaaga agtcggtttt
     8401 ggcatgacaa gagaaacgct caatgatcta gctgctttag gagttcatac agttgatatc
     8461 agcggtcgaa gcggaacaag cttcacccag atcgaaaacg cccgtcgctc caagcgggaa
     8521 ctgagttatt tagctgactg ggggcaatcg acagtctctt cattgcttga agcaaatgaa
     8581 gcagacactt ctatggaaat cctagcctcc ggaggtatcc gcaatgccta tgacatattt
     8641 aaagcccttt gtctcggcgc aaatgcagtg ggtacttcag gaaccgtact gacgcatctg
     8701 atgaatcacg gcgtagaaga aactattata ctgatgaagc aatggcaaga agagcttcgt
     8761 ctgctttata ctatggtcgg cgcaacgaat accgcggccc tacaccaaca atcactgatc
     8821 ttttccggac cagtgaagga ttggtgcgaa gcaagaggga tcgacttagt gaagtatgga
     8881 agacgcacag aaaagagcgt gggacaaaag taaagaacac ttttatccca cgctctaaac
     8941 acgtataaac ggcggaagca aaagcaactc cttcggaaat aagctgaaat tcacaaaaat
     9001 ttgaaaagca attttcgtga attccttctt atttcttgga gttaaacgct tctgtcccag
     9061 cctctttttt cgaataaatg atgtttaatc agctgtcctt cggtattcgt tcaagccgct
     9121 gatttcacaa cctctccact aaacggtgta ctaaagctct tttaaggttg ttttccgata
     9181 taggcaagta tgcctccgtc cacatacaag acatggccgt taacgaaatc agatgcgcta
     9241 gaagccaaga agacagatgg tccagccaaa tcgattggct cgccccagcg tgctgctggc
     9301 gtacgtccaa cgataaattg atcgaatgga tgacgttcac cgttttcttg tgtttcgcgt
     9361 aatggagcag tttgcggtgt agcaatataa cctggtccaa ttccattaca ttggatattg
     9421 tattgaccgt attcagaagc gatgtttttc gtcagcattt ttaatccgcc ttttgccgca
     9481 gcataagcac tgaccgtttc gcgtcctaat tcactcatca tggagcagat attgatgatt
     9541 ttaccgccgc ctttttcgat catatcccga ataactgctt ttgaaacgat gaatggggca
     9601 tttaaatcca cgtcgattac ttggcggaag tctgccgcat ccatttcgat catcggtgtc
     9661 cgtttgatga tccctgcatt gttgactaga atatcaactg aacctacatc ttctttgatc
     9721 tgttgaacca tcgcttgaac cgcttcttca tcggtcacat cgcagacata accttttgca
     9781 tcgatgcctg cttctttata atgcgctata ccttcatcaa ctgattcttg attcaagtta
     9841 ttaaatacga tttttgctcc tgctgtagct aaagagcgtg caatctcaaa tccaatacca
     9901 tagacagcac cggtgactaa tgctactttt ccatctaaac gaaacatatc catactaaat
     9961 tccatcattt tctctccctt atgcttattt taattgatcc atttgcacca tatccatgtc
    10021 tgtataggtg atattttctc cgcacattgc ccagatgaat gagtaattgc ttgtacctac
    10081 acctgaatgg atcgaccagc ttggcgaaat aatggcttgt tcattgtcta cgactaaatg
    10141 ttttgtttca tctggtttgc ccatcatatg gaacacacgt gtatcttcat tggcataatc
    10201 aaaatagaca taagcttcca tacgacgttc atgtgtatga caaggcatcg tattccaagc
    10261 acttcctggt tctaaaatcg tataacccat ttataaatga cagctatcgc tgaagcttct
    10321 actgctgtaa atatccccgt caagatacca ccgataatga tgacaatcaa tagaaggcta
    10381 ggaatcgcct cttttattat ttttccagca tgagccatct gtcctttttc gacaacaggg
    10441 tatttattct ttttagcaac gatgaatgct actagcatga ttcctagcgc ccacaaa
//
LOCUS       DAZUMK010000093        10453 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000093 DAZUMK010000000
VERSION     DAZUMK010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.095"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(79..2199)
                     /locus_tag="ACG808_002154"
     CDS             complement(79..2199)
                     /locus_tag="ACG808_002154"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS5047761.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYENSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2429..3607
                     /locus_tag="ACG808_002155"
     CDS             2429..3607
                     /locus_tag="ACG808_002155"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFS5047762.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3623..4381
                     /gene="aroD"
                     /locus_tag="ACG808_002156"
     CDS             3623..4381
                     /gene="aroD"
                     /locus_tag="ACG808_002156"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFS5047763.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4404..4889
                     /gene="ybaK"
                     /locus_tag="ACG808_002157"
     CDS             4404..4889
                     /gene="ybaK"
                     /locus_tag="ACG808_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFS5047764.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4960..6213)
                     /locus_tag="ACG808_002158"
     CDS             complement(4960..6213)
                     /locus_tag="ACG808_002158"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS5047765.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6329..7678
                     /gene="gdhA"
                     /locus_tag="ACG808_002159"
     CDS             6329..7678
                     /gene="gdhA"
                     /locus_tag="ACG808_002159"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFS5047766.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7790..9136
                     /locus_tag="ACG808_002160"
     CDS             7790..9136
                     /locus_tag="ACG808_002160"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFS5047767.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9444..9830)
                     /locus_tag="ACG808_002161"
     CDS             complement(9444..9830)
                     /locus_tag="ACG808_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS5047768.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(10098..10247)
                     /locus_tag="ACG808_002162"
     CDS             complement(10098..10247)
                     /locus_tag="ACG808_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047769.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
BASE COUNT         3329 a         1887 c         2104 g         3133 t
ORIGIN      
        1 ttattctaag atttctcttt accaacatca tttgacaaag agcctaattt tgtcgctgct
       61 ccttttaaat ttattttatc attcggaaga acttgtggtc gttccgtgaa gttcttggta
      121 cgtttgcgtt ttataaagat caacagttga ttgtcccttg tgttcactaa agacactagt
      181 tgacttgtca ccaagagatt tttcagcttc ttttaatttc tctaagccat tcttgtagtc
      241 ataatcactg gtatcaattg ggtctaatcc actattcgta tagaaccgaa gaagatcacc
      301 attattgatt tggtcagaaa cagctaactg tttatcaact ttttctttca gtgaatcaat
      361 ttcattctgt acttcctcag taggttcagt aatagcttca ccagtttcgt tactatagat
      421 cgtaccaccg tagtaagtat attttggtgt aacaaaatca ccatcacgga aagaaacaag
      481 ctgttcatga tctttcgaga atagatcttg tcctaactga atataatttt ttgtatctac
      541 acctagtaag tgaagtaatg taggcaaggc atcaacttgt ccgccgtaag tgtgattgat
      601 tccgccctgt gtttgtccag ggatatggat catataaggg acacgctgca tttgtgcatt
      661 gtcgtagttt gtccaagtag aacttgtttt tccaactaat tcagctagat ttttatttct
      721 tgaattcgag acaccgtaat ggtcaccgta aaggacgatg actgaattct catacaagcc
      781 gctttctttc aaataattga agaattcttg tacggcggta tctaaataat tggctgtcgc
      841 aaagtaaccg ttgatcgttt catcatttgt attggctaac gggaatcctg cttcgtcatt
      901 ggtgaatttt gaataaggat aatggttaga tacagcaatg aattttgaat aaaatggttg
      961 ttgcagacgt tccaagtatt gtacggattg ttggaaaaat ggtttatcat gcaacccgta
     1021 ttggaacgaa ttgtcatcat taacatcata ataagaagaa tcaaagaaat aatcatatcc
     1081 taaattcttg tatgtctcgt ttcgattcca gaatgtacca gcgttcccat ggaataccgc
     1141 actagtgtaa ccttgtgttt gacttaaaat atcaggtgca gcttggaaag tattttttcc
     1201 gcctaattgt gtgaacaagg agccttgatt caagccaaac aatgaatttt ccatcagcgt
     1261 ttccgcatcg cttgtttttc ctgcttttac ttgatggaaa aagttgtcaa aactgaacgt
     1321 gctgttgctg tggtaaagac tattgatgaa aggtgtaact tcaagctgga gaccattctc
     1381 atctgtcaat ttataatcga tcaggaattg ctggaagctt tctaaatgga tataaatcac
     1441 attttttcct ttagcgagcc cgaacaaatc atcatttggt gctgcataat gttcttttac
     1501 ataattttct acttctttca tatcattggg acttgcttct gcccgcactt ggtttgtctg
     1561 gtaagtttga acaccgtcat aaaccatgaa agcattcaag ccaagatatt tgaccaaata
     1621 atcacgagag aatgttcgag tcagcaattc tggacgatca gcttccgcca taaaaagatt
     1681 tcctgagaaa atcataactg ctaatgtaga gatcgcaaaa gccattcttg ctcggatcgg
     1741 gcgagggtcc aacttgattt tttttgcaat cagcagaaca gtcaagataa taaaatcgat
     1801 ccaatagatc acatcatatg ggcgaaacag tcgaatggca ctttctccca gaccactcgc
     1861 tactttccca gcacctaaaa tcgtgttgat cgtaatgaag tccgtgaatt ctcgataata
     1921 agtgacattc gaaaaaacaa gcaatgtcaa aagaaaataa atgaccatca tcgtgatata
     1981 agcaactcta gggcgtctga tataaagagc aattccaagt aataacaatg tagttgcaat
     2041 agggttgata aacaagataa agtattgaat gccactttcg attccgagat ggaaatcaac
     2101 taaataggca aaaatcgttt tcaaccaaag taaaacaact agtaaagaaa aaaatccgag
     2161 cctcttattc aaaaaggcag gtgtttttat acatttcaag ataatcttcc tttctgcact
     2221 tcttaatcac agaagtgtca tgaaaccatg ttacacagct cacttattct catttatttt
     2281 actagtatga caatatagtg tcaattgaaa ccagaagttt atgctgtttc attaaggttt
     2341 tctttaagct tcatgaaaaa atgagcagta agacaagtcc tatgtttttt gttatacttg
     2401 ggaagagaaa gctaaagcag gtgaaggaat gaaagtaaaa gtaaaaaaaa gagcaagcaa
     2461 tcgattgaaa aaaggatatc cactgatcca aaaagaagac cttgctcatc cccaaaaaga
     2521 aaccacgcaa tgggtggaat tagttgattc gcaaaaccaa tttatcgcaa aaggatatct
     2581 aggaaagcag aataagggaa tcggttgggt attgagccag cagaacatcc catttgatca
     2641 atcttttttt gaagaatttt ttgtgaagtc aaaagaaaaa agaactttct ttttttcaga
     2701 tgattctact accgcttttc gtatattcaa tggagaagga gacgggatcg gcggtctgat
     2761 tgttgattat tatgcaggtt atgctgtttt ttcttggtat aacgaaacgt tatatgcaca
     2821 tcgtgaatta ttgattcaag cttttcgtga aatctacccg gaaatcgtag gaggctatga
     2881 gaaaattcga tttgaatcag taaatctgcc tgagagccag ttcatttacg gaaaagaggc
     2941 accggaaccc ttgatcgtaa aagaaaacgg agtaaaattt gccgtttatt taaatgaagg
     3001 attaatgact gggatctttt tagaccaaaa agaagtacgg ggatcattag cagaaggttt
     3061 cgcttctggg aaaacagttt tgaacatgtt tagttatacc ggcgcctttt ctgttgctgc
     3121 cgcaatggga ggggcaatcg aaacaacaag tgtcgatcta gcaaaacgca gtttaaaaaa
     3181 aacacaggaa cagtttgaag tgaatggttt ggatgtcgat cagcaaaaaa tcattgttat
     3241 ggacgtattc gagtatttca aatacgccaa aagaaaacaa tttttatatg acatgattgt
     3301 attggatcca ccaagttttg ccagaaataa gaaaaaagtc tttcgcgtag caaaagatta
     3361 tggggaactg gtcaaggata gtctggatat tttagcagaa gacgggatac ttattgcgtc
     3421 tacaaatgct gcgaatgtct cgatagaaca attccaaaca atgattgaag aggaattcca
     3481 tgccaaaaat gtttcttttg aacaacttgc tttatatcgt ctgccaaaag atttccagac
     3541 gacggatacc tttttagaag gcaattatct gaaagtgttt gtttatcaaa ttaaaaataa
     3601 ccattagaag gagcgatgca gaatggtttg ggtaaaggga ttgaatatag gaagcggtag
     3661 accaaagatt tgtgcgcctc ttatcggggc cgtccgagag gaagtattac gtgaagcagt
     3721 tcttgcacgt gaagcagggg ccgatttagt cgaatggcga gtcgatcatt atcgtgaagt
     3781 tttttctttt gatgaagtag cagaaacatt ggttcttatc catgagatgc ttgatggact
     3841 gccgttatta tttacgtttc gtaccttagc tgaaggcgga gaacaagaca tatcgatccg
     3901 tcagtatcgc gacctttatg aacatgtgat acacacagga ttagtagata tggtcgatat
     3961 cgagttgttc aaagtagaaa gtctaggaaa aagactgata gaggaaatca agtctttaaa
     4021 cattccttta attatcagta gtcatgattt taaagaaaca ccagcagacc ctgtcttgct
     4081 ttaccgattg aatgttatgg aacattttgg agccgatatc ggaaaactgg cagtaactcc
     4141 caataatgaa cgagatgttt tgcggttgat ggagttgact agacgggcaa atgcttttgt
     4201 ctccatgcct attattacga tgtccatggg aaatcttggt aaaatttctc gtctggctgg
     4261 cggaacgacg ggttccgtta tgacatttgg ttccttaagc agcgattcta cttctgctcc
     4321 aggacaatta ccggtcaaag cattgaagca aattattcaa ttattaaatg aaccgttgta
     4381 gaacaagttt gaggtgatat tttttggcaa agaaaaaaat agtaaaaacg aatgcaatcc
     4441 gtatggtcga acaaaaaaag attccctata cagagcacga atatgagtgg gacgaaagcc
     4501 acctaagtgc gtcatcggtc gctgaacagc ttccagaaag ccaatctcat atatttaaaa
     4561 cactggttgc tgtgggcaat gtgacggggc ctcttgttgc tgtgataccg ggagaagccg
     4621 aattgaatct aaaaaaatta gcaaaagttt cagggaataa aaaagtagaa atgctccatt
     4681 taaaagattt ggaagcaacg accggataca tacgtggtgg ctgctcgcct ataggtatga
     4741 aaaaactatt tcctacttat ttagatcaaa ttgcagaaag ctatgaacaa atcattgtct
     4801 cggctggcag aagagggcta cagatggagt tagcacctca agatatctgt gcattaacga
     4861 gcggacaatt tgcagatata aaacaataaa tcatacgtat aaaagaaaga aggaggccgg
     4921 gatgttgttt gtccagtctc cttctttctt ttttatacat tatgctcttt gcccggcatt
     4981 cattcctgct acatgccctg tagcgaaagc cgctgtgata ttaaagccgc cagtataacc
     5041 attgacatcc agtaattctc ccgcaaaata cagaccatca atgattttac tctccaatgt
     5101 tttaggataa atttccttta gagaaacccc tccgccagtt acaaatgatt tctcaatagg
     5161 aaaagttttg ttgattgata gcttaaactc tttgcatagt tgaacaaaat cttgaatttg
     5221 tttttgagag gcttgttgtc ctgtcagatg atccatttca agccgctcaa gaaagaaaac
     5281 taacaatcgc tcaggcaata taccatgcca agcattgacc aagttctttt tggttgcttt
     5341 acttttttct gtcaatatat gaataagctc ttgtttggtt tgtgtaggga aacaatccag
     5401 agaaagtgtc actggttctc ccgttttctc taattcttta ttgacaaaac ttgaacaacg
     5461 cagtgcagcc ggaccagaaa taccgaaatg ggtgaaaagt aaatccatcg tatgttcagt
     5521 caatacacga ccttttttat ttaaaacgcg taaagtgatg tcttgtaaag aaagcccttg
     5581 aagtgttttc tcctgaatat acggttcatc cgatatcaag ggggactctg tcgcataaag
     5641 aggcgtcacc gtgtgaccca ctcgcttcac gatcttgtac ccatctccag tagaaccagt
     5701 agatggataa gtccgtccac cagttgttaa gataatggca ggagcatgga actcttcgaa
     5761 ttccgtgcgt ataccgtaaa ttttatgttc tttgtggatc aatttctcta ctcgagtaga
     5821 gaatagaagc gtcacatcta gttctttcag acgattgagc aatgcttcga taatcgtttt
     5881 tgatttattt gttacaggaa acatccgtcc atgatcctct tcttttaaat gtacgccttg
     5941 tgattcaaag aagttcatga tatcgaaatt attccattgc gcaaacgtac tatataaaaa
     6001 ctttccattt ccaggaatat gtgcaatcaa atcatcaaca ggacgattgt tcgtaacatt
     6061 gcaccgacca ccacctgtca tcagtagttt ttttcctgct tttttgtttt tttcgatcaa
     6121 gagtacgcgt gcgccttgtt cagctgcact gatcgctgcc atcatcccgc ttgttccagc
     6181 gccgactacg atcacatcat acagtttttc cattccctca cctcaaggaa agtgtagcat
     6241 atctgaagaa agaaaacaga aaaaatgttt tgagccaaaa gagaaaatga gctaaaatga
     6301 gggaagataa gcaaaggagc gtggaacgat gacaaacgca acagaatatg ttcaagcaat
     6361 tcaagaaaag ctacatcaaa aggatcaagg gcaagaagaa tttttacaag caatcgatga
     6421 attcatgccc acggttatgt cttttttgga cactcatccc gaatatatcg aaaagaatat
     6481 cttagaactt ttaacggaac cagaaagagt tattcaattt cgagtgcctt ggcaagatga
     6541 tgaaggaaac tggcgagtaa atagagggta ccgtatccaa tacaactcag ctatcggccc
     6601 ttataaaggt ggattgcgct tccatcctag tgtcaatcta agtatcttga aatttttggc
     6661 ttttgaacag attttcaaga atagtttaac cggtctgcca atcggtggag gaaaaggcgg
     6721 aagtgacttc gatcctaaag gaaaatctga taatgagatc atgcgttttt gtcaaagttt
     6781 catgctggaa ttgtcaaaac atatcggccc atcgattgat gttcctgcgg gagatatcgg
     6841 tgtaggagct agagaaatcg gctatttgtt cggcgaatat aaacgattga aaaaatatga
     6901 tactggtgtg ttgacaggta aacctttgga tttttgggga agtaagatac gtacggaagc
     6961 tactggttat ggattagtgt attatgtcaa acatctattg aacgaagaaa aagattcatt
     7021 taatcaaaaa acagtttttg tttccggaag cgggaatgta gccatttatg caatcgaaaa
     7081 agtgcaagag ctaggcggga aagttgttac atgttcagat tctagcgggt acatctatga
     7141 tccagaagga atagatgtga aactattgaa agaagtcaaa gaaatcaata gacaacgttt
     7201 gacggaatac gcaaaccaac gtccttctgc tatttatcat gctggagaat ctgtttggac
     7261 attgaaagaa aaagcggata tcgctttgcc atgtgctact caaaatgaaa tagacgaaag
     7321 cttggccgaa atcttagtag aaaatgggat caaaatcgta gcagaaggcg ctaatatgcc
     7381 atgtacagta aaagctgttt ctgttcttca tgaagcaggc gtgtggtatt gtccaggaaa
     7441 agctgctaat gctggaggag tagcagtatc tgctttagaa atgagtcaga acgcccagcg
     7501 attgacttgg gaaagagagc aagtggatga gcagctggat caaatcatgg aaacgattta
     7561 ccatacgtgt cgccaaacag cagccgagta cggcgacgaa aaaaatctgc ttcttggagc
     7621 aaatgttgct ggatttgaaa aagttgccaa agcaatggca gcagaaggtt tagtttgaaa
     7681 aatgaaactt tcttggaaat attgtcagca ttttgtttat ttgcttgaaa aataataaaa
     7741 aaaaacgtaa agtattacct gtagagtaaa cctataagga ggagaacaaa tggcacacat
     7801 tcattttgac tattcaaaag tagcaccgtt tgtcaacgaa caagagttag ggtacatgca
     7861 aagccaagta acagcagccc acaacgaatt acgggaaggt actggcgcag gaagcgactt
     7921 ccgcggctgg atcgatttac caacgaacta tgacaaagaa gaatttgcac gtatcaaaga
     7981 agcagctaaa aaaatccaat ctgattctga agtattagtt gttatcggta tcggtggttc
     8041 ttatttaggc gctcgtgcag cgatcgactt cttgaatcac acattcttca atttattaga
     8101 tagcaacaca agaaaagcac cacaaatctt ctttgccgga aattcaatca gctcaacata
     8161 tattgctgac ttgatcgaag taatcggtga tcgcgacttt tcagtaaacg ttatttcaaa
     8221 atctggaaca actactgaac cagcgattgc gttccgtgta ttcaaagagc ttcttgttaa
     8281 gaaatatgga caagaagaag caaacaaacg tatctacgct acaacagata aagcaaaagg
     8341 tgcagttaaa gtagaagcag atgcagaagg ttgggaaact ttcgtgattc ctgatgacat
     8401 cggcggacgt ttctctgtat taactgctgt tggtttgtta ccaatcgctg ttagtggtgc
     8461 agatatcgat gcattgatgc aaggtgccgc tgatgcaagc aaagcatact caagcgacaa
     8521 actagaagaa aacgaagctt accaatacgc agcaatgcgc aacattcttt accgtaaagg
     8581 caaagtaact gagttgttga tcaactatga accaggaatg caatacttct cagaatggtg
     8641 gaaacaattg tatggtgaat ctgaaggaaa agaccaaaaa ggcatctatc cttcaagcgc
     8701 aaacttctca actgatttgc actctttagg acaattcatc caagaaggac gtcgcaatat
     8761 ttttgaaaca gttgtgaagg ttgaaaaacc tcgtaaatca atcacaattc ctgaacaagc
     8821 agaagattta gacggtctag gctacttgca aggaaaagaa gtggacttcg taaatacaaa
     8881 agcatttgaa ggaacattgc ttgctcatac agacggtgac gtgcctaact tgctagtcaa
     8941 aattccagaa atggatgctt atacattggg ttatacaatg tacttctttg aaattgctgt
     9001 aggtatttca ggttacttga atggaatcaa cccattcgac caaccaggtg tagaagctta
     9061 caagaaaaac atgttcgcac ttctaggcaa accgggcttt gaagatcttg ctaaagaatt
     9121 gaacgaacgc ctgtaagata agggtttttc gctagattta aataatcagt gatagaaaca
     9181 tgttcgcttt aaaatgaaaa ctggtaccca tttaggtact caaacatact gcatcgttta
     9241 ttcagaataa ggggctgtga caagacgttt gtcacagtcc cttgtttgta tagaaatcga
     9301 ttgattgcct tgctctttag tttgttttag gactttaaga acagtgaggc tgacttcgaa
     9361 ggttaattct gtttttttgt tttctagaat cagtaagcaa aaaagcactc acttcgagtg
     9421 cttttttgcg atatattctt agcttaagat aggcctaatt tttcttttac agatgagggt
     9481 accttatcgc tggtaattac ttttttctct atgatccgct tttcgcttgc tttcaactgc
     9541 aagatcgttc cttcggtgta agcagaacca ttggcagagt caccagaagt aatgacatcg
     9601 aaatctaact gtcttttttc accgttttca gcataagcat ctacgttgta tttaaaacct
     9661 cttccaatta cttccccatt atctgctttg atatcagttt ctgcaggcat tggagctttg
     9721 actaccgcat aataatcttt acctacataa gtagactgat agtagtccca acctttccat
     9781 cctataaaag caatcaatat gataacgatc aaccctaaaa tttttttcat gaaagatctc
     9841 cttttttatt tttattatat taaatattat acagataata acggaatatt atcatcgaaa
     9901 cagctaacaa aattgtcata aaaaacgaga tggttgatat tagaacttgt gtttttgttt
     9961 aggtctagag ataaagagag acaatttcca aatatatgta atgaatacaa aaagacgttt
    10021 gtcgcggatt accgttacaa acgtctttta ttgattttcc gagatctcat cagtcgccat
    10081 ttggaatggt tcactcctta tctagattcg tttaatgctc ggactgcttg ttcgattgta
    10141 ataccaaacg caacgttatt ttcgttttgt gcatcaatcg ccataaaacg gttagaagtt
    10201 gtatcaaaag ctacgcggta ttgcatattt gtttttgtaa acgtcataaa attcatctcc
    10261 tttgtaatgt aatttagttc ttgaaagttt cctttaaact ttcgaactta taccaagttt
    10321 agcatcgtac gttttttgat gcaattaaaa agcatttatc attttcctta tttatttttt
    10381 cttaaatggt ttagctattt tcttttaatc cgataagtag cccaatcatc aaggttacaa
    10441 atactattgc ttg
//
LOCUS       DAZUMK010000094        10441 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000094 DAZUMK010000000
VERSION     DAZUMK010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.096"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            13..636
                     /locus_tag="ACG808_002163"
     CDS             13..636
                     /locus_tag="ACG808_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047770.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1086..2135
                     /gene="trpX"
                     /locus_tag="ACG808_002164"
     CDS             1086..2135
                     /gene="trpX"
                     /locus_tag="ACG808_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS5047771.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2132..3013
                     /locus_tag="ACG808_002165"
     CDS             2132..3013
                     /locus_tag="ACG808_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5047772.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3016..3786
                     /locus_tag="ACG808_002166"
     CDS             3016..3786
                     /locus_tag="ACG808_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5047773.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4065..4919
                     /gene="aroE"
                     /locus_tag="ACG808_002167"
     CDS             4065..4919
                     /gene="aroE"
                     /locus_tag="ACG808_002167"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFS5047774.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5097..6260
                     /locus_tag="ACG808_002168"
     CDS             5097..6260
                     /locus_tag="ACG808_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFS5047775.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6377..6787
                     /locus_tag="ACG808_002169"
     CDS             6377..6787
                     /locus_tag="ACG808_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5047776.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6961..9189
                     /gene="topB"
                     /locus_tag="ACG808_002170"
     CDS             6961..9189
                     /gene="topB"
                     /locus_tag="ACG808_002170"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFS5047777.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9360..9644
                     /locus_tag="ACG808_002171"
     CDS             9360..9644
                     /locus_tag="ACG808_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047778.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9804..10040
                     /locus_tag="ACG808_002172"
     CDS             9804..10040
                     /locus_tag="ACG808_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS5047779.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10037..10435
                     /locus_tag="ACG808_002173"
     CDS             10037..10435
                     /locus_tag="ACG808_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFS5047780.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3632 a         1760 c         2218 g         2831 t
ORIGIN      
        1 gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca agaaatgaat
       61 gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg tcttgttttt
      121 ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga aaaaatccga
      181 gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt gaaacctttt
      241 gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt gatgttcagc
      301 aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc tgagcttatg
      361 gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca tgaactggaa
      421 caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg aggaaaagaa
      481 gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga gttgatccct
      541 gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt ggatagaacg
      601 ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat gaaacggtgg
      661 gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc aaaataacaa
      721 ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact taaatcaatt
      781 cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag aaaacaaaaa
      841 aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc atagatacaa
      901 ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt gactgccgca
      961 tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc cattgtcttt
     1021 ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt tgcaggagga
     1081 aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt tatttagcag
     1141 gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg aaccagacaa
     1201 aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta gatgagattt
     1261 atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa aatcttgaga
     1321 tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt caacagctag
     1381 tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa tcattagcaa
     1441 acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg ggggctggat
     1501 tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat cagccgcctg
     1561 ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa gtcggaatct
     1621 tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa gaagctgcaa
     1681 aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag attgcacaaa
     1741 cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat aataccattg
     1801 ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc gtcattccat
     1861 ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat caatatcaat
     1921 tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa aaaccggaaa
     1981 cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct caagctgatc
     2041 gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt acagatgact
     2101 cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt cacaaggcat
     2161 gctttgggca atattaggat tagggatttt catgacttat cggatcttag actttcctga
     2221 tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag ctattactca
     2281 tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga tagcaggact
     2341 cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag ggattttagt
     2401 gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc tttctttatt
     2461 gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt actttgacac
     2521 agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat tcttcttcaa
     2581 tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg ctcggtcgtt
     2641 agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg gtgtcattgc
     2701 attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata aagggattgg
     2761 cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc aagaattgac
     2821 gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc tattgatttt
     2881 agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg ctatcatctt
     2941 agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa cgggttttga
     3001 gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa agtcgtaaac
     3061 aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat cagagaaggg
     3121 gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt caatacgatc
     3181 gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct gatcacaaaa
     3241 gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc caagatggga
     3301 acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag aggtcaaaaa
     3361 cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag actatgtcaa
     3421 gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt atctggtggt
     3481 caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt actattatta
     3541 gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg attaacaaat
     3601 cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat ggaagacgcc
     3661 ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca agatatttct
     3721 gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga agaagtagtg
     3781 gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca ttgacatctt
     3841 cctttgattc atcctatact taaaacaata aacaattaaa catatttggg tgttttgtag
     3901 gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg acaagtaaac
     3961 gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc gtatcaaacg
     4021 ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt tctggacaaa
     4081 ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca ttgatgcaca
     4141 atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa gtaaatccaa
     4201 tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt gtaaatcttt
     4261 cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag gaagcgcaat
     4321 tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc tataataccg
     4381 atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa aagaaaaaaa
     4441 tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct gcgttagacg
     4501 gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa gtaaaagaaa
     4561 cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac tttgccgata
     4621 cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca acagatatcg
     4681 ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg catgagaatc
     4741 tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa gaagcaaaaa
     4801 aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga gcaatcgcgt
     4861 ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg tttgattaaa
     4921 aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa aagtaatcct
     4981 ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa tgataggctt
     5041 ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga accacaatga
     5101 gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag cgggatacgg
     5161 ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag gaagatgcta
     5221 cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac gagcaacaga
     5281 tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt ttcaatcggc
     5341 aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa atcattctat
     5401 cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt aaatatgtag
     5461 ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg caaaaagcga
     5521 cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa gatgccgggc
     5581 gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg aaactgattt
     5641 cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag atcaattcag
     5701 cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat tttggaatcg
     5761 attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc gatgccttat
     5821 tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt gtagatatta
     5881 aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt ttccgaatgg
     5941 atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac gctattttca
     6001 gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt aaagctttag
     6061 gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt tcgattgcac
     6121 gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg aattatgggc
     6181 aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa tctgctatta
     6241 cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca aggcttttgt
     6301 catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga tttaaccaag
     6361 agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc gtgaagccat
     6421 cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat acggtgaaat
     6481 gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca cgaatgcacg
     6541 gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa ttggtcctga
     6601 attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag aagaaacgaa
     6661 gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg atgctgttcc
     6721 gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc agattaacca
     6781 agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg gatcatcgtt
     6841 tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt tattgccaga
     6901 tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg aggtcagaag
     6961 atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc cgaaaccttc
     7021 caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt tgctggtgaa
     7081 gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg agcttatgaa
     7141 gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac cttccgttac
     7201 gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt gcagtcagcc
     7261 gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc gtggtcgatt
     7321 attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg gatcaattct
     7381 cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa gaactatttt
     7441 ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg aatgaatgga
     7501 agtccattat atagcttgct acttcaacga aaagggattg atggcagttt ttctcttggt
     7561 cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat caaacacttc
     7621 aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg gcagttttct
     7681 ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa ggaaatagaa
     7741 aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac gaaaaaggaa
     7801 aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa gatgaatcag
     7861 ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta cgaaagcaag
     7921 tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca tcagtatctg
     7981 gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca gacaactgag
     8041 atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca tgcaattatt
     8101 ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt acagcaagca
     8161 atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta tatctacgaa
     8221 gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg aaagatccca
     8281 ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga agctcagact
     8341 cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat ggagaaagaa
     8401 acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa aactgcggga
     8461 aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg aatagggaca
     8521 gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat cgaagtcaat
     8581 aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt agccaatgaa
     8641 ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag aaaaattggt
     8701 aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat cctacattta
     8761 ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat tagcgggaca
     8821 cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc aaaatgtaaa
     8881 aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga atgcgacttc
     8941 aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag agatttgctt
     9001 tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg gaaattcgat
     9061 gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg gatcgaaaac
     9121 agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa tgaaaacgaa
     9181 gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg agaatataaa
     9241 taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta ggctgatttt
     9301 atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta aattatctta
     9361 tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct gattacggga
     9421 cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg attttatcga
     9481 ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca agcatgctaa
     9541 agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc attatttatt
     9601 ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac gagagtttgt
     9661 gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa aagcaaaata
     9721 aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact atagaatgag
     9781 aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt agaaaatctg
     9841 gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt gtgcagacag
     9901 gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc aaagcacaag
     9961 aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa gacgcgttaa
    10021 aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa tcaacgcgaa
    10081 agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa atgcattaga
    10141 tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc ctgaaatcta
    10201 tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc ataatggcaa
    10261 taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt gtccagttgc
    10321 tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt cattggattt
    10381 cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa aatgaacgaa
    10441 t
//
LOCUS       DAZUMK010000095        10212 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000095 DAZUMK010000000
VERSION     DAZUMK010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.097"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            141..818
                     /locus_tag="ACG808_002174"
     CDS             141..818
                     /locus_tag="ACG808_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFS5047781.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATIATTTTTSTESKPTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSESSEQVEQTLWNADKSAQLEAFMA
                     TWGQTMGQQYKSYTNQMSVDLYGLKVPQVILNGEWKMAIGGVPVSAEWSESGTGQADY
                     QITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELKNG
                     FNQIVYG"
     gene            1160..2380
                     /gene="sstT"
                     /locus_tag="ACG808_002175"
     CDS             1160..2380
                     /gene="sstT"
                     /locus_tag="ACG808_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFS5047782.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEPTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            2527..3162
                     /locus_tag="ACG808_002176"
     CDS             2527..3162
                     /locus_tag="ACG808_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFS5047783.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            3353..4096
                     /gene="truA"
                     /locus_tag="ACG808_002177"
     CDS             3353..4096
                     /gene="truA"
                     /locus_tag="ACG808_002177"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFS5047784.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYRS"
     gene            4330..5163
                     /gene="msrA"
                     /locus_tag="ACG808_002178"
     CDS             4330..5163
                     /gene="msrA"
                     /locus_tag="ACG808_002178"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS5047785.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDTRENISVKEQ
                     LSKLEVTLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQDERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKKIRKNHF
                     D"
     gene            5244..6237
                     /locus_tag="ACG808_002179"
                     /pseudo
     CDS             5244..6237
                     /locus_tag="ACG808_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            6526..7224
                     /locus_tag="ACG808_002180"
     CDS             6526..7224
                     /locus_tag="ACG808_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFS5047786.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNIA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNVLAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            7374..7829
                     /locus_tag="ACG808_002181"
                     /pseudo
     CDS             7374..7829
                     /locus_tag="ACG808_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            7910..9036
                     /locus_tag="ACG808_002182"
     CDS             join(7910..8138,8138..9036)
                     /locus_tag="ACG808_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS5047787.1"
                     /translation="MTRYEENFKQMIIELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAGSHILSENLNSLVQFIEKWCKDYTVS
                     LLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKE
                     GISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIILKENLLNQDFSTETICEKWAAD
                     ITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMIL
                     HTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDF
                     EEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
     gene            9081..10037
                     /locus_tag="ACG808_002183"
                     /pseudo
     CDS             9081..10037
                     /locus_tag="ACG808_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
BASE COUNT         3444 a         1629 c         2089 g         3050 t
ORIGIN      
        1 gcttcttagt ttttaaaagg tttaaaagat ggatgagaaa atacaaaaaa tcaataaaat
       61 cacattgctt atttgataga ataaagaagt gggagtaatc gaaaggagtt ttttaattta
      121 aaaaaaggat tttagtaaca attggtctag cttttttgtt agctggttgt accaacggga
      181 atgaatcgaa aaaagaatca acaaaagaaa cagcgaaagc aacaattgca actactacaa
      241 caactagcac agagagcaag ccaactgcag caagtacgga aacagcagat acctctactt
      301 cgtcagagtc ttccaaaacg actgaaacaa ctcaaacatc tacttcaagt tcttctgtag
      361 aggcttcatc ttcagaaagc agcgaacaag tcgaacaaac actgtggaat gcagacaaat
      421 ccgcacaatt agaggcattt atggctacat ggggccaaac aatgggacaa caatacaaaa
      481 gctatacgaa tcaaatgagt gtggacttat atggtcttaa agtccctcaa gttattttga
      541 acggggagtg gaagatggct attggtggag tgcctgtttc tgcagaatgg tcagaaagtg
      601 ggacaggtca agcagactat caaatcacag ccgtctattc cgatgcagag acagaaccat
      661 atctgaaaaa gcacgtctat ctattcgggt ttcagcaaaa tcagccaaaa gtattggtga
      721 cacaacaaaa tcaggggaat cccgataatt atctttactt taacgagaca gcgaataatg
      781 aattgaaaaa tggctttaat caaatcgttt atgggtaagt aaaccagtga gagtgagaga
      841 aagaagagac tcagtggaaa atattaattg attgctatgt ctaagaatta agatgaagga
      901 tgaaatttct agtttcgtcc ttcatcttag ttacgtttta cttggataac tataataata
      961 acgataaata gttttctctc gacttcttta gagattgaat aaaccctcca atactaaaat
     1021 tgaatgttgc aagagcatga attgccaatt gtattattag aatttttaga aaattgcacc
     1081 tttagtttct ttagataaaa tgatacaatg attgttaaaa ttgtgaaagg gtggtcattt
     1141 gttagagaag tttttggata tgtcgttagt tcaacgtatt gttgttggta ttttagtcgg
     1201 aatattgttt ggtatctttt tccccacgtg gacatttatt tcaatattgg ggacgttgtt
     1261 tgtcagttgc ttgaaggcaa tcgcacctat tctggttttt ttcttgacga tggcgtcaat
     1321 tgccaagcat aaaattggga ataagacatt cgtgaagcct attttgatct tatatttagt
     1381 gggaacattg ctttctgcac ttgtagcagt cattgtaagt tcaattttta ctgttcctat
     1441 tactttacaa gaaacagtat ctgaaaaagc accgcaaagt ttggaaagtg ttttatctac
     1501 tatgttgacg aatgtcacac aaaatcctgt tcaatctctt attgatgcga attaccttgg
     1561 cgttttattc tgggctgtat tgttgggact agcttttcga gcacgttcag aaccgacaaa
     1621 agagttggtg gatcagatat ctatagcgtt gtcacaagtc gtacaagtga ttattgcatt
     1681 tgctccgata gggattttag gattggttta tcaatcaatt gctacgactg gaatcgctgg
     1741 attggcagag tatctgcagc ttttacttgt tttgatcggt acgatgctgt ttgtcgcatt
     1801 agttgtgtac ccattcctca cgtttttgtt tattaaagaa aatccctatc cattgatttt
     1861 cttttgtttg aaagaaagcg cacttcccgc tttctttacc cgaagttctg ccgcaaatat
     1921 cccgataaac atgatgttgg ctgaaagatt gaaattgaca aaagagtctt attccatatc
     1981 gatcccgtta ggtgctacga tcaatatggg cggtgccgcg attaccattt ccttgatgac
     2041 gttgactgct gtacattctt tagggattga agcaccattt gttttgaaag tccttttatg
     2101 tattttgtct gctttagctg cttgtggagc ttcaggtgtt gcgggcggtt cactgttatt
     2161 gattccatta gcatgtagtt tgtttggtat ctctaatgat gtcgcgatgc aaatcgttgg
     2221 tattggattt attatcggtg ttatccagga ttcaatggaa actgccttaa attcgtcaag
     2281 tgatctgttg tttactgcaa taggagagct aggcgcgcgt aaaagaagtg gggaaaaaat
     2341 caaattgaaa gattgtttag atggattaag tgaacgttga acgttagata tttagtattt
     2401 ttaaaatgat tcgagttttt aatgagttat atagatgttc gtgtaaatag aggtttctgt
     2461 taaatcaaga taaagtcgtt tataataaca attaaaagca taggtaattg ttattggagg
     2521 cgtatcatgg attttttacg taaatttggt tttttctttt tattattagg cgtgctgagt
     2581 gattttttga ctccgtacgt attaggattg tattatcctg aattagatca aatgaagagc
     2641 gtgatcagtg tatttggaga ggtttccagt cctgtaagaa aagcgtttct tatttggtct
     2701 gtggtttcag ggatcttttt catcctgtca ttgcctgcat tgtatgctgc atttgtaagt
     2761 acatctcgtg tgttagctac cctagttaca gcagccatcg cgttctatgg tataggtgat
     2821 tgtatcttta caggtttgtt cagtatcaat acccatgaat cttcgtggaa tgtctcgaca
     2881 tgggtccata atatcggatc aggtttaggt tacagtggtt ttttattgtt tccagttctt
     2941 cttttcttga tctatcgaaa aaatgggcaa atggacgtaa gccacttata ccttggactg
     3001 tctgtgatca gtttgctttt tgcggcgatt tatggactgg caagaattcc ttctataaat
     3061 tcttggccat tactgaataa aataggtttt tgccaaagaa tcagtttttt ctttaattat
     3121 gtgcccattg cctggttgag tatcttgcat ctcaaacagt aaaacttgag ccgtcaaatt
     3181 gaacagtcag cggatacttc atccggtggc tgttttttgt ttttctgaaa tatatatttc
     3241 cacagttttc ttgtatagct tctgtcttag agaagcaagg agctatattt tgtgctacaa
     3301 tcttaaaaag gtagagagaa gaccaaatgg aagacagagg agccactaaa atatgagaaa
     3361 catcaagttg acaatcgaat atgacggaaa aagatattca ggatggcaaa gacttggaga
     3421 tgacgataaa accattcaag gaaaaatcga aaaagtattg catcagatga caaatgaaga
     3481 gatagaaatt attggatcag gaagaacaga tgctggcacc catgcgcgag gacaagtagc
     3541 aaattttaaa acaaacacag aaattggact ggcagaaatg atcgacttta tgaatcgtta
     3601 ccttccgcgg gatattgtca tcaaaagaat cgaagaaatg cctgagcgct tccattcaag
     3661 atataatgcg gtgggcaaaa aatacagcta ttatgtttgg aacaacgtga ttccttcagc
     3721 atttgagcga aaccacagct tttatttccc tcaagagttg gatatggaca agctgaatgc
     3781 agcttgcgaa aaattagtgg gtacacatga tttcattggt ttttctgcat tgaaaaaatc
     3841 aaaaaaatcc acagtgcgta cgattgatga attatctatc gaacgaaagg gagaaatgct
     3901 tcattttact tttgtcggag acggattctt atataaaatg gtgcggatta tcatggggac
     3961 attactagaa atcggcacag gtgccatgcc gatagaaaca atcgatgaaa tatttgaaag
     4021 caaagtgcgg caacatgcag gagaaaccgt ccctgcacaa ggtctgtttt tggatgaagt
     4081 ttattatcgc tcataaaaaa gaatcatctt tactcgactt ctgagaattg gagaacatcc
     4141 cttttttcag aggtcgtttt ttagttaaac caaaccatag agtatgcttt ttaagataaa
     4201 gatcatctta tatcatctga agctgcttat cttctttgaa taaatacgat tcaatcgatt
     4261 atttcctcac tttatgataa attacagcta aagtcactag cttgtcttct tcggaaagga
     4321 ggagaatcta tggatgagga acaaatattc attcaaacac tttataatct gattttgaat
     4381 ccgaatacac gagattggga acgaaaagta ttgattcaga caaagaatga cacaagagaa
     4441 aatatatccg ttaaagaaca gttatcaaaa ctagaagtaa cactacgtcc attagctatc
     4501 cgaatgaatc tgacgcctga tgtaatggac ttttatttat tgattacgga aggatttgat
     4561 aaggagcaaa aatatgactt ttccaagcat gccatgcaag atgctgacta tcaagaacgt
     4621 gcagtatttg cagggggatg tttctggtgt atggtcgagc cgtttgaatc gaaaaaaggg
     4681 atcctatctg ttttgtcagg atacacgggc ggacatgttg aaaaaccgaa ttatgatcaa
     4741 gtaagtggtg gttacacagg tcatgtggaa gctgtggaga tcatttatga cacaagagaa
     4801 ataagttata gcgagttgct gacaatctat tggcaaatta ctgatcccac agatacattt
     4861 ggacaatttc aagatagagg gaagcaatat cgtccagtga ttttttatca agacgaacga
     4921 caaaaagaat tagcggaaca atcaaaacag aaactagaca gttcaggaac tttccaccaa
     4981 ccaatcgtca caaaaatcga accagccggt acgttttggc cagcagaaaa ctatcatcag
     5041 caattttaca aaaaacaacc gaaacgctat aaaaaaatcc agcaagcacg taatcaattt
     5101 ctgatctatc aacgagtaaa aaacaaatgg caaaaaaaaa tacgaaaaaa tcattttgat
     5161 taactccttt tctttaaaat gtagtaaact aaaaatagaa aacaagaaag cgttttaaaa
     5221 agatttgatg aggaggagaa aaattgtata ttgcagatag tgcacgttat gaaaaaatgg
     5281 aatatcgtaa atccggtcat tcaggtttaa aattaccgat tttatctctc ctctcggatt
     5341 atggcaaaat tttggtgatt atgatcctat ccataatcaa agagagattt taagaggcgc
     5401 attcgacatg ggaattaccc atttcgacct tgcgaataat tatggaggcc ctgctggagc
     5461 tgcagagaaa aactttgggc gtatttttag agaagatttt caagcctacc gtgatgaact
     5521 gattatttct agcaaagcag gttatcatat gtgggaaggg ccatatgggg aatggggctc
     5581 tcgtaaatcc atcatatcaa gttgtgacca aagtttccaa agaatggggc tagattatgt
     5641 tgacatcttc tatcatcatc gcccagaccc tgatacacct ttggaagaaa cagcagaagc
     5701 gttgatgcaa cttgtaagac aaggaaaagc attatatatt ggtatatcca actataatgg
     5761 agaagataca aaaaaatgac tgaaatttta aaaagaaagg aagcaccgtt cattattcat
     5821 cagatgcgct acaatatgtt ttctcgtgca ctactagaag acgatctatc tccagtatta
     5881 gaagaagaag ggctaggggc aatcacgttc agtccgcttg ctcaagggtt gctgacaaat
     5941 cgttatctac atggtattcc tgaagattct agagcacatc gtaaggaaat accattcctt
     6001 tcagaagaac aagttggatc aactctggaa aaaatcaaag cactgcaaac aatcgccgta
     6061 tcacgaggac aatcattagc gcaaatagcc cttgcatgga atttacgaca aaaatctgtt
     6121 acaagtgtac tgatcggagc aagtcggtta agtcagttac aagaaagtgt tcgaatgatg
     6181 gataatttag atttctcacc agaagaagaa ttgagaatcg accaaattct agaatagaaa
     6241 cgatgtaatc aaacttttta tccatatatt aaaaaaaaca aaagatgaca tcaagaatcg
     6301 aatgaagatc gtctgttatt gaacatcata ttttttgtcg atcaaaataa aaatgatcga
     6361 aggctaggag tgaattttat cctagtcttt tttatggtaa atatcttctt cgtttgtatg
     6421 tctatgaacg gattaatcga ttttttatac aaaaaatcat cagatgattc aaacgtgtta
     6481 taataattac gaaaaaatac atcagatgaa aaggaggtat gtggtttgga agaaaataaa
     6541 accctagtag aagtaactac tgacaagatc cttagatatt taaaggaaat gcagttgcaa
     6601 gtaggagata aactgccgaa tgaatatgaa ttgtccgatt atttagaggt ggggcgaagc
     6661 acggtgcgag aagctgtgcg ggctctagct tccaggaata tccttgaagt tcggcaagga
     6721 gcggggacat ttgtcagtga taagcgaggg attgccaatg acccactcgg tttttctttg
     6781 attgaagatc aggatcggat gatacgagac ttatttgaac tgagatattt attggagcct
     6841 aagatggcag gtttggcagc ggaatatgca acgaagaatc aaatcgtcca aatgaaaaaa
     6901 ttaacgcaaa agattgaaac ttcttttcga gaaggcacac aagaacatgt tcaattagac
     6961 atcgattttc atacgatgat tgcagaagct agcggaaaca tagccttgct gcatattatc
     7021 ccaatcatta atgaatcgat tgctttgttt aataaaaatt atgatatcca gcatctgaaa
     7081 atcgagacga tcgatattca tacagagatc gttcaggcca tcgaaaggcg gaacgtactt
     7141 gctgcgtttg acgcaatgac tgtccatatg gcaataaatc ggagagaatt acagaaaata
     7201 ataggagaaa aagaaactaa ttgacagttc atctgatgta ttttatgata aatacatcag
     7261 atgatttttt gttttatatt tggtaaaaaa gaggagtaaa tagtttctgt tcaccaaaat
     7321 gtaagcgctg actaatagac aaaaagagat gacgtcaagg agaagaagca ggaatgaggg
     7381 gatctggaat gagttttatg gataaattag ccaaagtttt agggaacgtg gcaacgagaa
     7441 tcaataattt aaggtatatc atggtgataa aaaacgcatt tgctgcattg attccggtaa
     7501 ttattacggg tgcttttggc acattgtttt cagcaatggt ctttgactcg gaaaatggct
     7561 tagcacagat aagttacttg cgttttttag aagaattaaa gcctatcgca tctgccatca
     7621 gctatgtcac actaagtttc ttgaccatct atgctgtttt tctaataggg atcgaattag
     7681 ccaagctgaa taaagtggat ggtgtattcc ctggtatcat cgcagtgatg tcttatttat
     7741 ctgttaatcc aactgtttat gaattagtga atgaaaatca gactgttata gtagaaaacg
     7801 tattggctaa acaatataca gacacgaaag tggtgttagt atcaaatctt agacagcttt
     7861 tcgtagagac aagaataaat taaacttatc tacgagagga tgaattttta tgacccgata
     7921 tgaagaaaat tttaaacaaa tgattattga actgaatcaa actggacgtt ctgttcgagg
     7981 gttagcgaaa gaatatggct tatctgaagc aacgatttac aaatggaaga atttatattt
     8041 acctgatcag tccacaggac tgactggaaa agaagtagct gaactgagaa aagaaaatgc
     8101 tcgtttaaat gaggaacttg aaatcttaaa aaaagccgca gccatattct ctcggaaaac
     8161 ctaaattcgc ttgttcaatt tattgaaaaa tggtgtaagg attacactgt ttctttgttg
     8221 tgtcggttat tagaaatccc tagaagtgtc tattattttt ataagaataa accgttgaca
     8281 gctacagaaa tcagaaataa taagttgaaa aagaagattt caacaatttt ttttacgaat
     8341 aaacaacgct atggtgccac aaagatccat caagttttat taaaagaagg gatttcagta
     8401 tctcttaaac atgtccaaaa gctaatgaaa caattaaatt taaggtcgat tgtagttaaa
     8461 aaatatagac ctcaaaggtc taataaaccg atcattttaa aagagaatct cttaaatcag
     8521 gacttttcta ctgaaacgat ctgtgagaaa tgggcagctg acattacgta cattcctact
     8581 aagaaaaatg gttggtgtta cttatcttca atcatggatt tacacacgaa aaaaattatt
     8641 agttatacat tttcaaaacg aatgactgtg gattgtgtca ttcaaacatt gaataaagca
     8701 aaaatacatt atcacattcc agaaggaatg attctacaca ctgacttggg cagccaatac
     8761 acagcaagag aagtggaaca atggcttaaa accaacaaaa taaggcattc ttatagtaga
     8821 aaaggaacac cttatgataa tgctggaatc gaatctttcc atgcctcatt gaaaaaagaa
     8881 gaagtctaca cgactagcta ctcagatttt gaagaagcaa atcgagcgct atttagctac
     8941 attgaaggat tttataaccg aaatcgaatc catagctcga ttcactatct aaccccacag
     9001 gaatttgaag aattagcaaa agaaaaaatg gcttaaccgt ctctactaaa atgtgtccaa
     9061 gatattgact caaatccatt gatcgttacg gtgatagggt aaaaaactgg tttacattca
     9121 acgaaccaat cgttccccaa acgcgttgct atttggatgc tgttcgctgg ccgcatgaac
     9181 aaaacacaaa aaagtggatg atgtggaatt atcataaagt tttagcaacg gctaaagcag
     9241 tagagattta ccgcagcgga aattatggtg gaaggattgg ctgtatttta aatcctgaaa
     9301 tggtctatgc tcgatctaat tctccagaag accaaaaagc tgcttatatg tatgatttgt
     9361 tttataatcg agtatttttt gatccgatgg taaaagggta ctacacagaa gagttcctgg
     9421 agttgtgtca aaaacaatcg atttatttcg atcctgcaac agaggattta gaaacgattc
     9481 agcatcatac agtagatttc cttggaatca atcaatatta tcctaaacgc gttcgcgctc
     9541 ctcgttatca atggaatgaa gagacacctt tccatccaga acgatattat gaagtctttg
     9601 atttacctgg gaagaaaatg aataattcca ggggatggga aatctatccg aaaatcatgt
     9661 atgatatagc catgtatcta aaagaaaatt atcataatat cccttggctg ataacagaaa
     9721 acggtatggg aagagaacat gaagaacggt atatggatga gaaaggaatc gtgcaagatg
     9781 attaccgtat cgaatttatc ggacagcatt tgcgttattt gatcaaagca acagaagaag
     9841 gcgcaaattg cgaaggctat atgttatggg cattcactga ctgtgtgtca ccaatgaatg
     9901 catttaagaa tcgatatgga ttagttcgaa tcgaattgaa cgatgaacga agcagaacat
     9961 tgaaaaaatc tgcttcgtgg tatcagactt tgattgataa gagagaattg gatttgcctg
    10021 acgaaatcta taaataaata gaagtttttt gtagatgttc attgtattta ctggttattt
    10081 gaaataaaag aggttgtgaa aaagctgtcc tgcatcaagt aataagaata gcaaagtaaa
    10141 aacaacatct tatgttttta acttggttgg acttattacc gcagatgcaa gcttttgaat
    10201 accgtttatt cg
//
LOCUS       DAZUMK010000096        10190 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000096 DAZUMK010000000
VERSION     DAZUMK010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.098"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            60..209
                     /locus_tag="ACG808_002184"
     CDS             60..209
                     /locus_tag="ACG808_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFS5047788.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            220..1734
                     /gene="dltA"
                     /locus_tag="ACG808_002185"
     CDS             220..1734
                     /gene="dltA"
                     /locus_tag="ACG808_002185"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFS5047789.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1731..2936
                     /gene="dltB"
                     /locus_tag="ACG808_002186"
     CDS             1731..2936
                     /gene="dltB"
                     /locus_tag="ACG808_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFS5047790.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            2981..3214
                     /gene="dltC"
                     /locus_tag="ACG808_002187"
     CDS             2981..3214
                     /gene="dltC"
                     /locus_tag="ACG808_002187"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFS5047791.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3217..4485
                     /gene="dltD"
                     /locus_tag="ACG808_002188"
     CDS             3217..4485
                     /gene="dltD"
                     /locus_tag="ACG808_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFS5047792.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4626..5708
                     /locus_tag="ACG808_002189"
     CDS             4626..5708
                     /locus_tag="ACG808_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFS5047793.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5708..6511
                     /locus_tag="ACG808_002190"
     CDS             5708..6511
                     /locus_tag="ACG808_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFS5047794.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6581..7321
                     /locus_tag="ACG808_002191"
     CDS             6581..7321
                     /locus_tag="ACG808_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS5047795.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7332..8336
                     /locus_tag="ACG808_002192"
     CDS             7332..8336
                     /locus_tag="ACG808_002192"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFS5047796.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8381..>10190)
                     /locus_tag="ACG808_002193"
     CDS             complement(8381..>10190)
                     /locus_tag="ACG808_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047797.1"
                     /translation="SFYLRIKWSIIIILSLLFLTGIAYFFYFIFSPADSNQMKEIKDS
                     WVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYL
                     GEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTT
                     GPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGF
                     EAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLI
                     FIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLV
                     AIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKI
                     KNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNF
                     EMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHS
                     FRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLM
                     MMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3268 a         1691 c         2066 g         3165 t
ORIGIN      
        1 tgtttctttt tgtcaaaatt agttacactt tgtgtgaggt tatatgagga gggaatccaa
       61 tgaacaaatg gcgttctaat ccaaaattga attattgggg aaaattcatc attcgaacaa
      121 ttttttatag tgcagtactg ctgttcctga tttatctgta tcactataaa aatattcaag
      181 gaggaacttt tatctataac gaattttagg aggaagagta tggaaatcaa aacgattatt
      241 gaagcaatcg atgattgggg aataaatgaa cctgatcgag ttgcttatca ggcggatgag
      301 acgcatactt ttggtgaatt gaaacaagca tcagacgcac ttgcttatta tttagaaaaa
      361 aaggtagaag gcgatggacc aattgtcgtt tttggcaatt tggagtttga aatgattgtc
      421 tcatttctag gggttgtcaa atctggccat gcctatttgc cgattgaaga gcatacaccc
      481 aaggagcgta tcctatcgat ctttcgggtg gctaaaccat ctatggtcat cagtatcggg
      541 gactggatag aagatattcc ttcggttcct gtcataacaa aaaaagaatt tcaagaaatc
      601 aagaagatac cagtaggatt ttatcccaga aatagcgtaa aaggtacaga aaatttttat
      661 attattttta cttctggtac tactggagaa ccaaaaggcg tacaaatcag tcatgataat
      721 ctagtcagct ttgttcaatg gacgatggaa gactttggga tcaagcctgg tatgcatttc
      781 ttagcccaag cgccattttc ttttgattta tcggtatttt ctatttatcc tgcattagtt
      841 tctggcggaa tgctgaagcc gttaaataaa gcagttgttc aagactttcg tcaattattc
      901 gcgacattgc cagccttgaa gttgaatgtg tgggtttcta ctccatcatt tatggatatt
      961 tgcttgatgg aacctacatt caatggagaa aatgtgccgg aattggacat gtttttattt
     1021 tgcggagaag aattgacgaa aaaaactgca gaaagtctac ttgatcgatt cccaaatgca
     1081 cggatatata atacttacgg tccaacagaa gcaactgtag ctatttcaag tattcagatt
     1141 gaccagcatg tactagatag ttatgaccga ctaccgattg gctacgtaaa aaaagacacg
     1201 aaagtttcta ttgtccaaga aggtaaacct gtttcaaaag gagaaacggg tgaaatcatt
     1261 attgcaggac caagcgtatc aaaaggttac ctgaacaatc ccgaaaagac agctgctgcg
     1321 ttcttttgtc aaaatgactt agcaagttac catacagggg atgcaggtag actagatgaa
     1381 tatgggcttt tattttatga aggacgtatg gatcagcagg tcaaattaca tggataccgg
     1441 attgaactcg gggatatcga acattatctg ctccaagaca accgaatcaa acaagctgtc
     1501 gtcgtaccaa aataccaagg aacaaaagta cagcagctgg ttgcttttgt cgtattagaa
     1561 aacaaaacga aacagccgga ttttcagttg actaaatcta tcaaagaaca attactagaa
     1621 gttgtaatgg attatatgat tcctcagaag ttccattatg tcgatctgct gcctcagaca
     1681 gttaatggaa aaatcgatcg aaaaaaatta acagctgagg tgaatcctga atgatgttac
     1741 ctcatatgat cccttatgca gagccgtttt atttttttct tttagccatt gcgcttgtac
     1801 cgatcgttct atctttattg atatgggaaa aaagattacc agtttatcaa tcagcagtga
     1861 cacttttctt tttatttgtc agctttggag gtaattattg gcaacaggga gcggcgctga
     1921 ttgggtatgt tttgtggcag acgatacttg tatggggcta ttttgtttat cggcaaagga
     1981 aaaatcagac aggatggttt tttgccgccg ttattttagc gataattcca ctgtttttaa
     2041 cgaaagtgac tccttttttt gaacatggaa aagcatcgtt acttggtttt ttaggaatct
     2101 cttatttaac ctttaaatct gtacaaatga tcatggaaat aagagatggc atgatcaaaa
     2161 catataagcc atttcgttat atccagtttc tgctgttctt tccaacaatc tcttctggac
     2221 caatcgatcg ctataggaga tttgaaaaag atcagctcca tccgcctgtt cgtgataaat
     2281 atgttgatct tttgggaaaa ggcgtccaaa atatttttgt cggtttttta tataaattca
     2341 ttatcggtta ttattttggt caagtactgc ttcctgtagt gggacgcatt gccttacagc
     2401 atggtggtat ttcatgggca ttagtaggtt atatgtacgt gtacagtatg tatttgtttt
     2461 ttgattttgc tggctatagc ttatttgcag tcggtacaag ctacatgatg ggatatcaga
     2521 caccaatcaa tttcaataaa ccttttttat catggaatat caaagaattc tggaatcgtt
     2581 ggcatatgac gctgtccttt tggttccgtg attatgtata tatgcgattg atgttcttca
     2641 tgatgaagaa aaaatttttt aaaagcaaga tcgtaatgtc aaatatcggt tattttgctt
     2701 tgtttcttct aatgggtata tggcatggat tgacgtggta ctacatcgta tatggactgt
     2761 atcatgcttg tctgatctgt ctgacagatg catggatccg atttaagaag aagcataaag
     2821 agcagttgcc ttctaacaaa tttacgcatg gcttagccgt atttttgaca tttcaagcag
     2881 tatgttttag cttcctgatt ttttcaggat ttttagataa actttttttt agctaacgaa
     2941 tagtgacata ttaatttata taacaaagga gaaaaggaaa atggaagaac aaattttatc
     3001 aatcttagaa gaaataacag gaacagatga agtaagaaaa aatcgtgata ttgatctatt
     3061 tgatgaaggg ttgcttgatt ctttagcatc tgttcaatta ttagtcgagc tagacggaga
     3121 gctggatatc caagtccctg tatctgaatt cgagcgagag gattggagca caccgaataa
     3181 aatcattcaa caagcaactg cattgaaggg ttgagtatga aaaagaaact ttttgctatt
     3241 ttcggcccgg ttgttttagc tgttggctta ctgtttgtgt tttttacctc ctcctatcaa
     3301 gtagatatct ctaatccaga aacgattaga cgtgcctctt cttccatgtc agtgaacgtg
     3361 ttgaaaggag atgcgataaa aaattatgca ttatctgaaa aacaatatat tccttttttt
     3421 ggctcaagtg aattaagcag aatcagtcca tttcatccgt ctgttctggc agaaaaatac
     3481 cagcgaaact atcgtccatt tttgctggga gcacccggga cacaatcgtt aagccaatat
     3541 atgatgatgc gttctgcagg agatgctatg aaaaacaaga aagtcgtctt tattatttct
     3601 cctcagtggt tcgttaaaaa tggtgtaaaa actgattact ttaatacgta ttattctgaa
     3661 cttcaaacat acgactggtt gttttcaatg aagaaagtaa cgcctgctga tcgctattta
     3721 gctagaagat tattaacttt ctcaaaagta aaagagaatg atacgttgac ggcgattctg
     3781 caaacgatca aaaaagggaa gctaccttta cctgaatctt tgaatcagct gcgcagccaa
     3841 tggaacatgc tgaagagaga ggatgaagta ttcagcaata ttgggttgaa agatcgacag
     3901 caaaaaatcg atcatgagtc aaaacgatta ccaaaacaat accaagaaac agaattatcc
     3961 attttagcaa atcaaattgg agaacgagaa acgacgaata atccattcgg gttgaaaaac
     4021 gatttttata cgcatcgtat ccgagcccac gagccagagc tcaaacagtc acaaaaaaat
     4081 tgggattatc gtttttcgcc agaattttct gattttcagc tggtcttgga tcaattagca
     4141 aaaaaccata acgaagtatt gttcatcatc ccaccggtta acgagaaatg gagcgattat
     4201 accggactat ctcaagaaat gttacaagga tttgccaaaa agatcaaatt tcaattaaat
     4261 agccaaggat tcaatcgaat agctgatttt gtcaatcaag cgggtaccaa ctattttatg
     4321 gaagacacga tccatctagg ctggaaaggc tggttggctg cagatcaaca gatcagaccg
     4381 tttttagaag aaaatcatat tactgctagt aagtatcatc ttgacgatgc tttttttagc
     4441 aaatcttggc aacatcaaat accagataaa ctacaattaa aataaagaaa acaggcgagt
     4501 gttgctcgct tgttttcttt atttttgaat attctgaata aaagataaaa ataccaagtt
     4561 tgcctgaaca aaaatcataa gcaatggtat aatcacaata tgaatataga aatgggggaa
     4621 aacatttgga tacaaaagaa gaacagctac ttattgattt aacttctgca aaaggagtac
     4681 ctggaaatga agaagaggta agagaagtat tcagagaata tgccaaacca ttcgctgatg
     4741 atatttttta tgacggttta ggcagtgtga ttgccaaaca tggtgctggc ggaggcccca
     4801 aggtctttat ttctggacat atggacgaag ttggttttat ggtaacaaaa ataaccgaaa
     4861 aaggattctt ggaattccaa actcttggag gttggtgggg gcaagtcatg cttgctcagc
     4921 aagttgaaat caaaacacgt gaagggaaaa tcgtccacgg tgtgattggt tccaaaccac
     4981 cacatgtttt aacacctcaa gtaagaaata aaccttatga aataaaggat atgttcattg
     5041 atattggcgc atcaagccaa gaagaagcca aagagtgggg catacgtcct ggagatatgg
     5101 tcactccata tattgaatac aaacgaatga atggcagtaa atatttactg gcaaaagcat
     5161 gggataatcg tatcgggaca gctgtttctc tgcgagtatt agagaatcta tctaaagaag
     5221 cacacccaaa tgttttattc gcaggaagcg atgtgcaaga agaagtaggc ttgcgaggtg
     5281 ctcgaacaag tacacacctt gtaaatcccg atattgcttt tgctttagat actggtacag
     5341 caggagacac tccaggaatg acaccaaaag aagcagattc gattttagga aaaggcccac
     5401 agattttgat ttttgatgca tctatgatcc cgcataaaaa attattgaat tttgttattg
     5461 atgtagcaga ggaaatggat attccattcc aatatacagt gatttctggc ggaggaactg
     5521 atgcaggaca aatgcatttg acaagagatg gtgttccgtc tgtagccatt accgttcctg
     5581 ttcgttatct gcattcccat acatcagtga ttcatgaaga tgattatttg aatacagtga
     5641 aactagtcac agaattagtg aaacggcttg atagtgaaac agttgaaaaa attcggagtt
     5701 atgaataatg aataatagaa tacctatttc tgaatatcgg aaaaaagcac gagtttcttt
     5761 ggaaaatcaa tggggaatca atacttggct tgttttctta tctataatat ttacgagcgt
     5821 catccagaac ttttttgacg gtttcagtcc ttttccggaa gaaagcatgc agcagcactt
     5881 attgagtttt ctagtcgata atttactttt attcgcattc aactatgcat tgttttacat
     5941 tgcgctagta gttgtacggg gtggcagacc aaaaagtaac cttttatttt cagttttcca
     6001 aagaaagtat tttggttcga tacttggcat caatttgatc aattcagtca tcaactggct
     6061 actcaacagt ttaatctttc tgcccatgtt tttaatcagc ggagtgagta cttatatgca
     6121 gctgcttttc agttcaaatg cggtacctac gggtgtcatg ccagaagatt ttcttgatat
     6181 cagctttgtt ttatcgatga tagggatgat ttttgtttca gcactcatca tgagtattgt
     6241 tggcggttta ttccaatttg ctgcatggac aaagttcgac tatcctgatt tgtcggttat
     6301 tcaatgtttg aaatatggct ggtttttatt gaaagataga attggtacgt atattttgtt
     6361 gcagctgtct tttatcggct ggtatttgct aggagcaatc gctttgttca tcggtctttt
     6421 gtgggtcatt gtttatgtga atgtgtcagt tgcagaattt tatgaacaag cacggattga
     6481 aaaaggaggt ccagacgcct attttactta gcatgtaagc agtttattca tcaaaaaaat
     6541 aacttttgtg gtaggataaa tctttgaggg ggaatgtacg atgaaattaa ctgttttagg
     6601 atgcttagga gcgtatcctt ataaaaatca agggacaaca ggttatttgc ttcaatctga
     6661 cgggttcaat ttgttgatcg atgcaggtag tgctacgctg atcaaattac aagaacacct
     6721 tgatccactt gatctagatg ctgtgatcct tagccattat catcatgatc atattgctga
     6781 ccttggtgta ttacagtatt actggcaact tcatcctaag agaaatccgg agacattgct
     6841 ttcaatttat ggacacacaa aagattccat tcactttgaa gaattgacgt tggaaaatgt
     6901 ctcaaaagga catccttatt ttgaagctga agaattaaaa attggtcctt ttctgattac
     6961 ttttatggaa acaatccatc cagtagtatg ttacgccatg cgtatcgtag aagaaacaac
     7021 ggggaaagta ttcgtattta caggtgattc aggttatttg gaaagtttcg ctgattttgc
     7081 gaaagatgct gatttgtttc tagcagatac ctatcttttt gaaggaaatg aacatcatca
     7141 tgcccatttc acttcaaaag aagctggaga acttgcgaaa tcagctaacg taaagaaact
     7201 tgtacttaca cacctacctc agtttggtga tttagaacaa ttgaaaaatg aagcgcaaaa
     7261 ggcagcaggc aaagggattg atatagaact tgctgcagtt gacaaagtgt ttaacattta
     7321 ggaggaagaa catgattttt gtaccaaatg aaaaccacga cccaagaatc aatttagcaa
     7381 ttgaacaata tttgctgcaa gaaatgcctt tagatgaacc tattttgctc ttttatatca
     7441 atgaaccttc aatcatcatc ggcagaaacc aaaatacgct tgaagaaatc aatcgagatt
     7501 atgtggaaga aaatggcatt catgttgtcc gtcgcttaag tggaggcgga gctgtctacc
     7561 atgattttgg aaacctgaac tttagtttta tcatgcctga tgatggggat tcattccgtg
     7621 actttgcgaa agtaacacag ccaatcatcc aagcacttca tgaattgggt gttgctgggg
     7681 cggaattgaa aggccgaaat gatctagtca ttgatggcat gaaattttct ggcaacgcga
     7741 tgtatgctac caacggtcgg atgtttgctc atggaacaat tatgttcgac tctgatatca
     7801 atgaagtagt caatgcatta aaagtaaaaa aagacaaaat cgaatcaaaa ggaatcaaat
     7861 cgatccgttc tcgtgtaacg aatatcaaac catttcttcc agaagaaaag caaaaaatga
     7921 caacagaaga atttcgtcaa gaaatcttgt tgaaaatttt cggcgtagag tcagtagaag
     7981 aagtaaaaac gtacgaattg actgatgaag attggaaaaa aatcaatcga atctctgagg
     8041 aatattatcg aaattgggat tggaattacg ggaagtctcc agatttcaat ttctctcgcc
     8101 aaaaacgttt ttctatcggt tcgattgaag tccatttgaa tgtctcagaa ggaatgatca
     8161 aagaagcaaa aatctttggc gatttcttcg gactaggaga tatctctgac gtggaaaaaa
     8221 aattggttgg acagaaatat gataaagcat ctctatcaaa aatagtcagc caaattgatg
     8281 tgaagaaata ttttggtgcg atagaaccag aagaattgct taccttactt tattgaaaat
     8341 aaaaaatagc ggaatccaca tatatggatt ccgctatttt ttatacagga ttcgggtaat
     8401 ttcgagagag ttgaacagtg atccgatcaa tgaaagtatc tttttcctgc gttagtgttc
     8461 ctgtgtaacg atccgctacg tctttcatca tcattaagtc aaaacgttcc tgatcatcta
     8521 attcttctgt gatgcctcga ctaaataggt ttgtatcgta atgtgcacta tgttcgcaac
     8581 aaatgacgaa atgttcctct tgttcgtgta tcgacaaata aatttttcga tacagtggat
     8641 tttcgatggc atgagtttct ctaaaggaat gctctaaagc gtggatcaaa agttgagcca
     8701 gatcgtcatc gttgatagga agttcttcgg gaagatctgc ttggaaggtt atctgaatat
     8761 tccgtttggc agctaacttc tggtaacgcg ctaagataag atttgtcacc tggtggttgc
     8821 aaaaagaaca cagcatttcg aaattttttg cttcttctaa tatcttttcc acatagtttt
     8881 tagcttttgt ttggtccgta tgcattaaat cttgcagatt ctccatctct tggacaaatt
     8941 ccaggcgaag tgtatttact tcttggatat gctgtcgaag ctgctcataa tgttgctcca
     9001 acaaatcatt tttgattttt aagaaatcta cttgtctccg atctttgtcg gtatttaaga
     9061 tgtgatcgat tagattgtag ctgataataa cgattagaat gataatatat aaaatcgtag
     9121 accaattgat cgttgtttga tagataccta atgccgtttg gatgtaaatc aagcaatgga
     9181 taatggctac caagacgatc caaggtgtac aagctacata aaaacggtta ttcttatatg
     9241 cttcgccaat gcatgcaagt gaagtgacta aagtgcttat aacactagca atattagcaa
     9301 tcggcattaa ttcaggaagt tctgcaggag taaaagttgc ataaagcaga acgccgataa
     9361 aaatcaaaag ttggaacgta caaaaaattc cgtaccattt tttatagtaa aacattcggc
     9421 tccaatagta gcagatgatg aaagctgacg ttaagataat aaataaatga gataaaaaag
     9481 aatgaacgaa tggttcaaaa agaataccac ttaaaatatc ttctgataca gcttcaaatc
     9541 ctaatgccaa agcaaagcaa cttaagagaa taagtgagta ttcaagacgt ttttgtttat
     9601 acatcaagta gatcgtatag aaaaaggtcc ctatagcgat aaaaacagcg attatcatcg
     9661 tcaatacttg cggcactgat tgtgaataaa taaaactaat caatggtcca gttgttccta
     9721 gataaacttt cggtggcagt ccagcatagt tctgataagg agatgagact gtaagggtca
     9781 atttttttcc gatataattt gctggtaaat ctatcacagc caatgagagt ccgggattgc
     9841 tttcttcctg ataatctttc cgttggtata aaatcttttc gcctagatat acagtaatcc
     9901 attgatggtt tcctttgatt agtaaatttg cttcgtccaa cggtttttca agtgtcgttt
     9961 ccataatgaa tgtttcgtct ttatccacgg taggaatcgt acgagtgtac ttgctgtcaa
    10021 acttaaaatc aggatcatct tttgtataga aaacccacga atcttttatt tctttcattt
    10081 gattgctgtc cgcaggagaa aaaataaaat aaaagaaata agcaattcct gttaaaaata
    10141 acaatgataa tataatgatt atgctccact ttatacgtaa ataaaaagat
//
LOCUS       DAZUMK010000097        10094 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000097 DAZUMK010000000
VERSION     DAZUMK010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.099"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(558..782)
                     /locus_tag="ACG808_002194"
     CDS             complement(558..782)
                     /locus_tag="ACG808_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047798.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            973..3750
                     /locus_tag="ACG808_002195"
     CDS             973..3750
                     /locus_tag="ACG808_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS5047799.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3831..4250
                     /locus_tag="ACG808_002196"
     CDS             3831..4250
                     /locus_tag="ACG808_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5047800.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4262..4732
                     /locus_tag="ACG808_002197"
     CDS             4262..4732
                     /locus_tag="ACG808_002197"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5047801.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4749..5528
                     /locus_tag="ACG808_002198"
     CDS             4749..5528
                     /locus_tag="ACG808_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS5047802.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5515..6336
                     /locus_tag="ACG808_002199"
     CDS             5515..6336
                     /locus_tag="ACG808_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS5047803.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6353..7402
                     /locus_tag="ACG808_002200"
     CDS             6353..7402
                     /locus_tag="ACG808_002200"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS5047804.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7415..8419
                     /locus_tag="ACG808_002201"
     CDS             7415..8419
                     /locus_tag="ACG808_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS5047805.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            9052..10071
                     /locus_tag="ACG808_002202"
     CDS             9052..10071
                     /locus_tag="ACG808_002202"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFS5047806.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3390 a         1659 c         2100 g         2945 t
ORIGIN      
        1 tctataaatg aattctgcat aaaaaatata tcaagtaaat gtttatagat gaaactctat
       61 ttacttcttt tttaatttgt tcttgcaatc ttttttgcta cgtaattttt gtaaaaaagt
      121 gggttgagag aaatctaatt gattttcaaa gaaggagtat gaaaatgaga aaaatattta
      181 ttcgtttatt gtgggtacta tcttagagga ataaataata gtggagaaaa ctatggtttg
      241 gcgcagattc tatcaggttt gatggaacga agacaacctc ccgacatcaa gtcaaaagct
      301 acttgagatt ttgatctgaa aataaaaaaa gaaaacaagg taggaacatc aatgtttcta
      361 ccttgttttc gtaatcaatg ggaaaaaact aagagtggtg tacgtaatca ataaaaagga
      421 tggtgacaac agtacctctt tactctctga aagcatcata actcgttttt cctcattatg
      481 ccaataaatt gttgttatac cacatataag gatttgtaat agctaaaaac agacaggaaa
      541 gttgaaatct gtgaatctta ttttgtatgg aaggatttat agaacgcaaa aatcaagata
      601 aagatgaata aaaatcctgt ttcggctata tattgcgtat ctacgccaat aaaacgagtt
      661 tgttctgtca tagccatata aagcaaaggc aaaaatacaa ctacagcgag ataagatccg
      721 atgatttctc gaaaagtttt ccaggaagta tgtttcctgt ttttatattt atccgatttc
      781 attgaatagt catagcctcc ttattctttc atatgttgaa attccagtat aaaaactgga
      841 aggaactgta cgttttttag aataaaaatt ataagggata agtaggctgc tgtctataat
      901 aagatatatt tttcaattct ttattaaaga ttttattgac aaaatgaatc cgttttctat
      961 gatggaggag gggtgaataa gttgttgaag aaggaaattt tatgttactt agagaaccag
     1021 acagcctttt tcgatttaga acatatgaac gaggtcttta cagcacaaag gttagctgat
     1081 cattttgggg taaaaagaaa cacagtcagt cattatttga accagttaaa tgatgagcaa
     1141 ctactagtca aaatcaattc tcgaccagtg atttatttcc acaaagaagc ttttgaaaaa
     1201 caatttttta agttacgcac gaatttttat ttttctatcg atgatctcaa agaggaacag
     1261 ccttttttcg cgcaaccaaa agacttattc tcttttatga tcggatacga cgccagtttg
     1321 aaagaaagta tcgaacaaat caaaacagct ctttactatc cagatggcgg attaccactt
     1381 ttgattacag gcgaaagtgg cacaggtaaa agttatttag ttaatttggt ctatcagtat
     1441 tgtctgcttc atgatctgct agaagattct gcaccattca tcacttttaa ttgtgcacaa
     1501 tatgcagata atcctgaact tttgactagc aatttgtttg gacatgttaa gggagcgtac
     1561 actggtgcag aagaaaataa aaaaggggct tttgaagcag cagatggtgg gatcttgttt
     1621 ttagacgagg tacatcgctt aacaccagaa ggtcaggaaa aactgttcac ctatcttgat
     1681 caaggtgtga tttatcgtat gggagaacct aatttgtcac gccggatcaa aacacgtctc
     1741 ttttttgcta cgactgaaga gattactagt catttcttaa caactttcat tcgaagaata
     1801 cctatccgaa tcgaactacc tgcacttaat cagcgtagtc gtaatgaacg gctagaactg
     1861 atttactctt tttttctcga agaacaaaga aaaatgggga gccctttgtc agtcagtgga
     1921 ccagctttgt cacttttaac aagtcagaaa ttatccggaa acataggaga gctgaaaaat
     1981 atcgtcaaag tttcagcagc aaaagcatat gctgaacagc gtgaacagcc tgaaatccat
     2041 gtcacgatcc atcatttgca aaaagaacta cttgcgcagc ctattactaa aaatacagca
     2101 cagcaaggaa tctatattac gcaagaccag accttagagc aattaatgga gaagcaacag
     2161 cccgaacagc agcgaatcgt taaaagtttt gagcaaatcc taattgattt caagaaaaat
     2221 aactgtctgc tatattcttg cgaaggaaaa ttaaagcaag aagtgattca attatttgat
     2281 tttttgctgt ttgagaccag tagacaagaa aaacacgagc tgctggttta ttggacacaa
     2341 tctatccgag agacctttcg acaaatggaa actgcctatc aaatcaaatt cgatggaaac
     2401 agtgtctatg ccttgagtta ctatctctat cagcgaagca ctatcaaatg gttaccggaa
     2461 gaacatgata ttatccaact aatcaaagaa ttagaacaac aagtttctca gtcttatcca
     2521 tctagttatc actatgtcca acgaattctg gaattaagta agatcaaatt agatattgaa
     2581 gacacaagta tggatcggat tttactgacg atctatctaa aaaaagcgaa atggtcaaaa
     2641 gaaaaacgac tgccaaaagc gatgattgct gctcacggtt atgcaactgc tagcagtatg
     2701 gcaaatgtag taaatcgttt attacaagag gatctatttg aatcatttga tatgccacta
     2761 gatgtcacac cacagcagat tgcttcagaa atccttgatt attgcgaaaa cagcgatgtt
     2821 tcaaatggat tgatcatatt agtagacatg ggatctctga aagaaatcca tcagtttttt
     2881 aaaaaacagt tatccgtacc gcttttgatt ttgaataatg tgacgacacc tttagcaatc
     2941 acagtagggg aatgcctgca aaaaaatgta tcattagaag agatagcaga agagactgtt
     3001 cgacagatcc agccagaatg gcgactgctc tatccagagg aaaacaaacc caaagcactc
     3061 attactactt gcttcactgg aatcggtacg gcggtccact taagtgaact gctagaaaaa
     3121 agcttaccta cggcttgtca gttgaaaatc atcccatatg aatatcagca attaaaagat
     3181 aagaaaaaca gtgacccatt attttccata tacgaagtcg taggaatgat tggcaccaca
     3241 gatcctgata tcaccaatat cgactatctt tcattagaag acctcatctc gggggaaaag
     3301 atgtctgtac tagctgaatg gttggacact actatgaatg cttcagagaa ggaaatattc
     3361 aatcagcgca tcattcgtaa tttttcattg gaaaaagtac tggattccgt gactattcta
     3421 gatacagaga aggtgatggg agaaatcgat cattttatga gagaattaga ggtgcagttg
     3481 aatcgaagca tcagcaatcc caaaaaatta gcgttgtatg tccatgtgag ctgtctcatt
     3541 gaacgattga tccgacaaat gccaatcgaa acctatcaag gattagagaa atggaagcag
     3601 tgtcaaaaag aagggttatc tgctattaaa tctgcgttta gtgtcattga agagcattat
     3661 agtgtcatga tccccaattc tgagattgcc tatatctatg atattttgag tgaagataca
     3721 gagtttctct cgatagaaga agaattttga acataagcaa gttgttgcca ctaattttgg
     3781 catgcaactt gcttttttag tatgtgtaag tgaaaagagg agatgaaaaa gtggatagag
     3841 aaattatctt agcttcccat ggaaaatttg cttcagggat attagattca ttagagctga
     3901 tctatgggaa aaatcattct attactgtat tcgattgcta tactgatgag aattttgatt
     3961 tagcggaaac agtaaggcag ctttttatga ggtacaaaga taaagaaatc attgttctga
     4021 cggatctttt tggcggaagt gtcaacaacg aattcttaca atatatcaat cgggatcatg
     4081 tctatctcgt tgccggattg aatttgccac tattgattga gctagtatct cggttggacg
     4141 ctgaagtaga gacaactgtt ttgatccagc aggtgctaac tgattcaaaa aaaatgatcc
     4201 agttttgtaa cgagcggctc aatcagaaaa tagaagaaga cgaattttag gaggaagaaa
     4261 aatgattgta ttgacaagag tcgaccatcg gttattacac ggacaagtag ctttttcatg
     4321 gacacaaaca ataggcgcag actgtatttt gattgctaat gatgatgtac ctacgaatga
     4381 gatacgaaag acaacgatca aattagccaa gccacaagga gtcaaactag tgatcaaatc
     4441 aattgatgac tcgattgccg cgttgaaaag cggggtgaca gataaatata agttattcat
     4501 cgtagtggaa tcgatcgagg atgcatataa gctggcagaa gcgtatccag aaatcaaaac
     4561 catcaactta ggagggatca aggcaaaacc tggtactcgc agcatcggaa aagcggtaaa
     4621 tattttacca gaagaggagg aacgcttgaa acgattaatt gacaaacaaa tagaagtaga
     4681 aatccgtcaa gtaccagcag ataaaaaagt ggatgtaaga gaagttttgt aagaaaggaa
     4741 gaacctcaat gattgtacaa gcagtcttat taggaattgt tgcatttgtc gcacaatcag
     4801 aatatgcact agggacatcg ttgctttctc gtccgattgt gacggggctg tttacaggaa
     4861 tcgtgttagg tgatgtaaaa actgggatca ttatgggcgc aactttagag ttagcattta
     4921 tcggatcgtt ctcagtaggt gcttcgattc cacctgatgt tgtgaccggt gggattttag
     4981 ggacggcttt tgctattaca gctggagccg gtacagaaac cgcgctttta ctaggtttgc
     5041 cgattgctac gttgacattg atattgaaaa atatttactt agggcttttg attccaatta
     5101 tgaaccataa agcagacaat tatgcagaag aaggaaatta taaaggagtc gagcatatgc
     5161 acttgcttgc cggtatcggt ctttccttga tgcttggtgt tgtggtaatg gtttcttata
     5221 tggtgggaag caatacgatc ggaaatctat taaatatgat tcctgacttc gttcaaaagg
     5281 ggttgtcagt agctacaggg ttgattccag ctcttggttt tgcgatgctt gctcgtttgc
     5341 tgatcaacaa acaagttgca ccatattttt tcttaggttt tgctatagct tcttacttag
     5401 agattcctgt gaccggagta gctatttttg gtgcgatttt agcagttgtg gtagtcaata
     5461 ttatgaatgt ccgtcaacta ccacttcaaa caactgcagg ggaggtagaa gaagatgaag
     5521 aattctgaga tcaaaaacag caaaataacg aaaaaagagc taaatcacgt attttggcgt
     5581 tcttttcaaa tggagttttc ttggaattat gaacgtcaga tgaatatggc ttatgtttat
     5641 gcaatgattc cgattttgaa aaagttatat cagtcgaaag aagaaatggc agcagctttg
     5701 aaacggcatc tagagttttt taacacgacc ccacatatcg ttacactgat tttaggaatc
     5761 acggctgcaa tggaggaaga aaataaggat gatcctgaat ttgatgtgac tgcgatcgat
     5821 agtatcaaaa catctttgat gggacctctt gcagggttag gagattcttt cttttgggga
     5881 actttgcgat tgattgccac aggtgtgggt acctctttag ccatgcaagg aaatatttta
     5941 ggccctatct tgttcatttt gattttcaat attccgcaca ttctgtttcg ctatctagca
     6001 acaggttggg gatatcgctt aggaacgggt tttttgaaaa agatccaggc aaacggtatg
     6061 atggaaagtt tgacattagg cgcgtctatt attggactga tggtcgttgg agcaatgact
     6121 gccacgatga tcaccatcaa tattccttta aaaatcggtt caggagaaaa tgctgtgact
     6181 gttcaaagta tttttgatga tatcgttcca aatatcttat gtcttggagc atttggcata
     6241 gtgttttatc tattgaagaa agaagtgaaa ccattaacga tcttgatcgg actagctttc
     6301 tttggaattt ttggttcgtg gatagggatt ttttaaaaaa ggagcagaga aaatgtttac
     6361 gatgcaagat tatatctatg aagaaaagga agtactaagt acgatcttga aaaagaatga
     6421 tttttcgaca agagaacata tgaaaaaaac agtgaattta ctgatcttag caacgggttc
     6481 atcttataac gcttgtttag cagcgaagcc agcattggaa tcttatggtg atcttactgt
     6541 cgatattcaa gaaccttttt attttttcaa ttatggaaaa ctttctcctt caatcgatac
     6601 tgtgattgct gtgtcacaaa gcgggaaaag tgcttctact gttgaagcag taaaaatgat
     6661 acaaaaacaa gggttacctg ttgttgctat cacaaatgac gtgcaaagtc cacttgcttt
     6721 agaagctgca caaattattg atttaggtgc cggggtagaa tcagtaggct tcgttacaaa
     6781 aggctattct gcgactgttt tacagttact actcttaggg ataggaattg gaataagtaa
     6841 aaataaaatc agcaaaaaaa tagaacaaga ctacatgcaa cagttgcgca aaataatcaa
     6901 ccatttacca gcgattattc aaaaaacgga agaatttttt gatggatatc aaagtttatt
     6961 tcgtttagca cagcgtttcg tcaccattgg atatggtcca aactggggaa cagctaaaga
     7021 agcagagacg aaattgactg aaacaatccg cgttccgtca caaggatttg agttagaagc
     7081 ttatatgcac ggcccatacc tggaggcaga tgcttcgcat ttgctattct ttattgaagg
     7141 ggaaagtgta aataaggaac gttcgcaaaa gttgcaaaga tatatgagtc ggtacgttgg
     7201 agagactctc accattacca caaaaaaagc aagaaacgag aaaacacttg gattagcaat
     7261 agaatgtgat gaatatctgt ctgtattagc gcttgtcgtt ccgtttcaac tattcgctta
     7321 taaaaccgca gtagctaaag gtattgactt gaacaaaaag atttttgaag actttgatac
     7381 agtcttgaaa agtaaattat aagaggtgga aatcatgctg aaattcaatg aagaagaaca
     7441 aatcaaagca ataaaaggcg cactagcttt acgtccgcaa gtagaagaaa tcatcgataa
     7501 actttatgcg gaaaaatttg acgctgtcta ttatctagga atcggtggaa cgtatgcttc
     7561 ctcgatgcaa gctgtcactt atatgaatgg gaaaagcaac ttgcctgtct ttgtacagca
     7621 tgctgcagaa tattacacta ctggaaacaa aagattaaca aaagattcta tcgttatttt
     7681 gtcctctgtt accggtacaa ctcaagaagt agtgaaagca gtagaacaaa tcaagaaagt
     7741 aggagcgaca ctgattggat ttatcgataa agcgaacagt aagttgtctc agctatgcga
     7801 ttttgtggtc acttatccag ctcctggaac agaacaaatc aaatttttta tggcagcaga
     7861 tcgattgatg tatttgaatg gagaattcga agcttattca gaatactatg aacagctcga
     7921 acgttattta ccaacagggt tagtggaagc agagaaaaaa gcagatgcat ttggcttatc
     7981 atttgcagaa aaacatcgac gtgatagtat gcattatttt attggagcag ggaatcaatg
     8041 gggagctgtt tattcatatg ccatgtgtta ctgggaagaa caaagctggc ttccatcgaa
     8101 atctatccat gctgccgagt ttcttcacgg gacgttagag atcgtagaag aaacaacccc
     8161 agtcacatta tttttaggcg aagatgaaca gagagtgtta tctgaacgtg tggctaaact
     8221 tttgccaaaa atttgcagca attatacatt gatagacaca aaagactatc cagtagaagg
     8281 tatcagtgaa aaatatcgcg gacgtgtttt gtctttctta ttgatgcatg tagtgacaca
     8341 acgcatcgat gctcatgtgg agcagttgaa ctgccatcca ttagagatac gccgctacta
     8401 tcgtcagttt gattattagg atagactgga taaaaagaat ataaaaaata aagagagcag
     8461 cgataactgc tccctttatt tttttaaaat attggcatca attacgttat aaaacacatt
     8521 ttttgcatta tctacagttc aacttataat ctgtttaatg ggacatgtgg gtgccaacca
     8581 ttttttgtta tgcaatagta ctatttagtt gttgcatgga tccgtagtaa aagtccaagt
     8641 acaacgagtt gcgccagttt acatagcgtt ttgtatccag aatgttgact aaaacaaaag
     8701 ttcctatgtc aaccgtcaac agagacaatc tgtccgttgc aggacctgac agatttagaa
     8761 atgttcaatc tgtctcttat gtattttaaa tcaatactca ctaggagaag tttcctaacc
     8821 tcctaatagt aatgctggta gtaacattgt caaaactgct tcctcttagg aacaagcaaa
     8881 gcataacgag tgtaaaaata aaatgtagac tattgtaaca tatcattgat tatttcaagt
     8941 aaaaacgata aaagaagtaa acaaacactt aaaaacaatg gaaattagtc ccaactttaa
     9001 gaaatgtata ctataatgga aattatcaat tatttatttg gaggaatatc catgaaacaa
     9061 aaaatcgcag ttcttggtcc tggatcttgg ggcactgctt tagcacaaac attggcagaa
     9121 aacgggcatg atgtccgtat ttggggaaat gtacctgaac agattgatga aatcaataag
     9181 tatcatacaa atcagcattt tttacctgat ttgacgatcc cagaatcgat tatcggatat
     9241 aaagaattgg cagaagcagt ggataacgca gatgctattt tatttgttgt tccaacaaaa
     9301 gcgattcgtt cagtcgcaca ggaacttatt ggtaaaatga atactaagcc aatcattata
     9361 catgcaagta aaggactaga acaagatacg cataaacgaa tctcagaagt tttggctgaa
     9421 gaaattccag aagaaaaaag acaggcaatt gttgtattat ctggtcctag ccatgctgag
     9481 gaagtggcag tacatgatat cacaacaatt acagctgcaa gtattgatca aaaagcagca
     9541 gcctatgtac aaaaactgtt catgaatgat tatttcagga tttatacaaa taatgatgtg
     9601 attggcgtag aaactggtgc agcactgaaa aatatcattg caattggtgc cggcgctatc
     9661 catggattag gattcggaga tgatgcaaaa gcagcaatca tgactcgagg attggcagaa
     9721 atcagccgtc ttggtgtcgc gatgggtgcc aatcctctga catttatcgg tttgagtggt
     9781 gtgggggact tgatcgttac ttgtacaagt gttcattctc gcaattggcg tgccggtaac
     9841 ttgttaggac agggacataa gctggaagaa gttctagaaa acatgggaat ggtcgttgag
     9901 ggtgtggcaa cgacaaaagc agcagtagag ctagctcaac agctaggtgt tgagatgcca
     9961 attactcaaa caatctataa tgttttgtat aatggagaag atatcaaaaa agcagcaaaa
    10021 gaaatcatgt tacgtgacgg aaaaatggag aatgaatttt ctcttcgtta aatagatgat
    10081 caatagttaa tcgt
//
LOCUS       DAZUMK010000098         9986 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000098 DAZUMK010000000
VERSION     DAZUMK010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.100"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1043)
                     /locus_tag="ACG808_002203"
     CDS             complement(<1..1043)
                     /locus_tag="ACG808_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047807.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNT"
     gene            complement(1267..2151)
                     /locus_tag="ACG808_002204"
     CDS             complement(1267..2151)
                     /locus_tag="ACG808_002204"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS5047808.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(2246..2683)
                     /locus_tag="ACG808_002205"
     CDS             complement(2246..2683)
                     /locus_tag="ACG808_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047809.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            complement(2900..3811)
                     /locus_tag="ACG808_002206"
     CDS             complement(2900..3811)
                     /locus_tag="ACG808_002206"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS5047810.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(4268..4696)
                     /locus_tag="ACG808_002207"
     CDS             complement(4268..4696)
                     /locus_tag="ACG808_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047811.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(4724..5362)
                     /locus_tag="ACG808_002208"
     CDS             complement(4724..5362)
                     /locus_tag="ACG808_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5047812.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(5366..7348)
                     /locus_tag="ACG808_002209"
     CDS             complement(5366..7348)
                     /locus_tag="ACG808_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFS5047813.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            7528..8193
                     /locus_tag="ACG808_002210"
     CDS             7528..8193
                     /locus_tag="ACG808_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5047814.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            complement(9684..>9986)
                     /locus_tag="ACG808_002211"
     CDS             complement(9684..>9986)
                     /locus_tag="ACG808_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS5047815.1"
                     /translation="LGVKGQNLLADDSFWQIEAFYLAQCAYNTTLMLSPDRIIFGGGV
                     MKQEHMKKKVQDKFVELINGYVEIPPIASYIVTPELGDSAGIIGGLALARKAIRNK"
BASE COUNT         3139 a         1935 c         1539 g         3373 t
ORIGIN      
        1 tttgtattta ccgaaaattc gcttcccgaa ccactgactt caaacatgac attaccaggt
       61 agcgtatcga ctgcctctcc ttcatcgcta acgccatctg cccgatcata atcaagagag
      121 acctttgaac cgcttaagat atggattcct ggcgctgttt ttccactacc agtctcatct
      181 cctgaatcta gaaaaccgta gaatttccct agatacggtc cataaagttg actaccattg
      241 atcacattca attgatagct tctacaaaaa taggctttac ccggagcggc attcagtcct
      301 tccttcactg gttggacatc caacacaccg gaaacaccat ctaatgtgac agttgcacga
      361 ggagcgtaaa tcagtcctaa tttggaccca tcatacaaat caagtgtccc ggtaaatttt
      421 atttctcccg taaaatgata tctgcttccc caaaaatcgg tcatataagc aatgataatg
      481 gcatttccat cgatatttgg tgtcaatcca cctggatgtg cagctgttcc tttaaaattc
      541 tggaactcga tcgactgagg tatctcattg atataaatat ggtgtgtgcc gaaattgtga
      601 gcgaatccac caaaatcaat aacgatatct cgtttgatct ctaattccct gtctaaagtc
      661 aaaggctgaa tcagtcggat cttagttact gattcgtctg cgagaagctg cgccagttct
      721 tctgttgtgg cagcttcgct gtacccatcc gctgaaacag ctcttgccag tgccgcttca
      781 ctgctatcag acgtttgttc agctacttct gaggtctcac ttgtcttatt ttcttcttgc
      841 cttttattag tagaagcctc actttgagat tctgtagttt cagaagtaat cgattcttct
      901 gaaaaagtag tcttgtcaaa actttgattc tctgattgcg ttgattcgtt tgttttctct
      961 acatcggcaa aaacggatgt caccatcaag agagggaaga caaacaaaat cgacgcaatc
     1021 agctttttta cacattgatt cattctccac catcctctct gacgttttga caattttatt
     1081 ttatcgtttt aatctatttc caattgtact ttcatattta aaatatgtat ttttatggaa
     1141 aaatttttct attaggtgta aatttctttt ttgtacaaaa aaaaggctgt gacaaaagtc
     1201 ttgtcacagt cccttatccc gaacaagcgg tgttctcccg ccaactcctc ggtcacaacc
     1261 tccattttaa caataaacct gtaaatgtga gggcaatact ttgattgtca caggtaattt
     1321 atccccttca tctccatcaa cattcgtcgt caattcttcc tcatcagcta atgacacaac
     1381 taattgctca caagtaaggt aggctagatt tttcgtagat tcatctactc cttttaatag
     1441 gtctggcaat gttttaagcg tatcgatcaa agaagaatct tttaaataaa ccaaatggag
     1501 cttaccatca tccacttttg catttggaag taatgtttca aaaccgccga ctgagttagt
     1561 tagtcctatc agcaaggtac tgctctcaat ttcttccttc tttccatcaa cttctacgtg
     1621 aaacgagtag gattgtgtgg aagctaactg tttgattcct gacatgaaat aagctagttt
     1681 tccaaatttc gtcttcttct ccgcatctac atcattgatt gattcaggga tagagccaat
     1741 tgcgacgaca ttcataaaat agtcgtcatt tatttttcca atatccaatg gcttgactga
     1801 ttcaatcgaa aaatgattga tcgcttcctc aggttctagc ggtatcccta atgctcgagc
     1861 caagtcattc accgtaccca atgggaaaaa tccgaaattt ggccgatgtt cttgttcagc
     1921 gattccactg attccttcgt tgactgttcc atctccgccc atgacaaaca cactatgata
     1981 cccttccgta gccgcttcgc gagtaaagtt ttttgcatcc cctgcttttt ttgtatgcaa
     2041 gacgaccact tcatcaaaca ccgattccaa ttttgctatc gctaattgct caaattcctt
     2101 tgcttgttca ccgcctgaac tggggtttac cactaataaa acttttttca tcactttctt
     2161 tcccttctga ttgatttctt taatataacg atagcataaa gaaaaaaatt aggctgtgat
     2221 gaagtactgt tcatgaaaca ttcatttatt ttttgacaag ataaaacatc catactatga
     2281 aactgaggaa aattaaaaca atgatatagg aaataaaaga aaatgatagg cattgtatac
     2341 ctaataaagt aaaaatctta ggtggcagat aaaaacacaa aaatagcaag agaatgtaat
     2401 agcgtttctt ccactttttg ttatcagacc ttatagcgat cagaggaata ccaaatgcat
     2461 aactgaaaaa ctcttgatgt actgttaata tgtcacggcc ttgaataata aatgcgacaa
     2521 tccccgctat aattagtgtg cctaacacca aaacggaaaa ttgatgaata tattttatat
     2581 acaaatgatc cattgtactt cctgcctttt tatttgtgag aaaaacaaat ccgtatacgt
     2641 gaataacttc tagaaataaa agaaatttgc aaaaccactc catttatcta cttctacgtg
     2701 cattttttga tattgttttc tttatttaag attatacaga aaaatatcta gggtcgctgg
     2761 attctaagta aaaaaaattt ttaaaataaa aagaactaaa tcaagtagta attaaactac
     2821 tgttgattta gttctaataa aaagatttag atgaacagtt ttattgattt aaatagttca
     2881 tattttttat ttataccgct tatttactac ttgctgtact agaatctgaa gtgaatgtac
     2941 tcaaaacatt ttcaaagtct ttgtctttga tttttacatt agcgtctttc aatacttcgc
     3001 caattacttt attctggaag gtactgtcac ttaattttgt gtctgttgca atttctttca
     3061 attctttttt gtatttatcc atgtcgttgc ctttgttttg gtttttcacc attttaacca
     3121 cataatattc cgtagtataa gttgaagcat tggtagaagt gatcacatca gaaacttgac
     3181 catctttcaa cttaaaggct gcttctttga cttctgccgg aaccgttgta gtagtggaat
     3241 caaatttcac ttttccgcca tcttcttttg tcgtagtatc agttgacttg tctttggcta
     3301 atttagagaa atcgtctccc ttatcagcag ctttcttcac atcttttgca tcatcttcac
     3361 ttgctacttg gataatttga gcttccactt ctggatggaa agattcccaa gccgttttta
     3421 aatcttcatc ggtcaatttg atatgtttct ttagaccttc ttgaaaggct agactttgtt
     3481 tgatactgtc tttgaatgtt tgttctgtgt aacctgcaga tttcaattgt gaatcaaatg
     3541 aatcacccaa ttgtttcttt gtttgatcat attgagaatc gatttctttg gttgatacgt
     3601 catctccata tttatcttca aatacttgat aaacgatcaa ctgttgcaca gcggaatctg
     3661 ctccttgcat ttttttcaag ctatccatta cttcttgttc gctgattgta ccgcctttat
     3721 aactaacggc tgttttttca ccggcattcg tacagcctga caataagcca gtgcctagta
     3781 cgatggcaag tgctaaaaat ctttttttca tttatcaatt ctcctacttt tcgttttttg
     3841 cgaattgcta aaaacaaagg tgttcaagat gaaaatcatc aagaacacct ttactaatct
     3901 gctcgatacg tcctgaatag atataaggct acacaatcca gaaagttcgc tcagcagaat
     3961 aaatgtcaag tagcacatca caccttaata tactacttct tttgttaccg tttttcaagt
     4021 ctatcttgcg ttccgtattt aacgtaacta attcttagaa caaatgttat cgattttgtt
     4081 tttcttcttc cgctgctggg atattcaaat cagacacttt tatgttcgat tttccagaca
     4141 tttttttgtt gtttttatta tgatatattt ttatacaact aaaaatgaat atattaaaac
     4201 taccaaaaat aacttttaac gctgtttcat tatatgtttt ttctatttta tataaacttt
     4261 tggatactta ataccaaccg gttggattgt accaacatga gaatgtgtca gttctatttc
     4321 ctgtcgcact tgcataggcc catactcctt ttgttgcatt tgctgatcga atagagtttc
     4381 caactttcgc ttttgcaccg tgacgattat agacacgact atagtggtta gaatgacctg
     4441 tttttctcgt acaagttgta tcactataat aaacccatct tccagagcta tgattaattg
     4501 ctgttctaga aatttcccgg tctactggta aatttgactt aataatatta cctttagcat
     4561 caaaatgaat ctctcctggc tttaaagatt tgtaatagat ttcaccgtga actgaatttg
     4621 cttccacttc agatgacata gcgtttcctc caacaaaacc tatccctagt gcacaaataa
     4681 taactgcttt tttcataaaa cgacatcctt tcctttttga tttttaaagc atgatcttct
     4741 tgtttcctat tttggtcaga tatggatcat gcgtgactac gataactaac gtaccttggg
     4801 aagcttgttg cgataagatt tcaactactt tatccttatt tcttttatcc aatgacccag
     4861 tcggttcatc ggctaaaatg attttgctat tcttcaataa gactctcgca caggcgattc
     4921 tttgcttctc accaccagat aatgtgtaga taggcgtact tagcttcttg tttatattta
     4981 cttttttcaa tgcctcatac tttgctgttc gcttatctga aacatttaaa ttcgaaaact
     5041 tcaatccaat atctaaatta aaatcgattg tttcatattc aatcaaccca aaatcttgaa
     5101 aaagaaaaga tatggtattt ctttttatca agcgcttctc tctcccagtt aattgtttcg
     5161 tctcttgatc gaacaaatag aattgtcctg aactgaactg atcattcaat cctaatatat
     5221 tcaataacgt acttttacct gctccacttt ctccaacaac agtcacaaga tccccagcat
     5281 ccgcagtgaa acgatccaaa tgaagaatcg ttttgtttcg aatgattttt ttcgcacctt
     5341 ttatcactat caacggctcc aattgttaac ctccattcat cagcaataac tgacttttct
     5401 tttctgtaga caaaataaaa acaactgaaa gaataaaatc aatgaagcta aagaaaaaaa
     5461 tattaaaaag atatttaggc tctctttcta taagaaaaag gaagattaca cttgtcgttc
     5521 cccatttgat gaggttcatc atgaagaaat ctttatacgt ttcaaagagc gaataaccac
     5581 ttgaacgaaa gatagcgatt ttccgacaat tgtagacaaa atatatgcat aacatgacat
     5641 agctcaacaa gaagaaagaa aagaaagtga gtaaaaatat ctgtatcaag taatgaaatg
     5701 atctgcttaa cactttgaca gctatctctt cagtctgagt atacggaaat gataactgca
     5761 agttatcacg taactgactt tcttcaatca gctcaaataa cctttctttc gacaaagagc
     5821 ctatcttgat ctttaacggt ggatagattt ctccgctgag aatatttctt tccaaataca
     5881 tactgttttt caatgtcagt atttcaacaa ccggataatg ttcgatccag gggatagaag
     5941 gaataaatgt ataaatgggt tgattgtttt ttataaatag aaatttcggt tctttttcac
     6001 tgatttcttg ataatattct gttatttgcg ccaggtcatt ttttaaatct gtatgtctat
     6061 atcgttcagg gatcaatata acagggtgcg tttctttttc ggttatttgg atcaaccttc
     6121 cagagacatc aatcaaagga aatttcttaa gataattcag atttattttt atccctctgc
     6181 caaactgttc attttcttca ttcaagtaat ctgatacatt gactaatata cttccgtttt
     6241 tattcaaagt ttcatataat tcctgatctg ctgctgatgc aaaacgattg gaataaataa
     6301 aatccacgtg gtttttcccg atggttactg gataaaatac atgatagtcc tcaccagcga
     6361 tttcttttgg tggttcatgg gaaacagcta actgtgaaga agaaaaaacg atagttgcta
     6421 attcgacatt ggcaacggtc aaaagaagag aaaacaacaa tccaaatgta aaaagaatgt
     6481 attggatata gttgaacgct tctcttaacg aagcgatttt ttcaactaat aaaagagaaa
     6541 tcaaagagat accatatatt ccgctgaaca agaaaacgta catctggaac aaataaagaa
     6601 attgctcctt ataatacata aaaataaaac caccaatgat tcctgcaatt atgaatgtaa
     6661 taatgatttt ctttttcccc aataaacacc acaatacttg atgtaatgaa aagtcattat
     6721 tcaacaataa actgatttct ttattcatca ataagaaaaa ggatgataac aaaaggaata
     6781 aaagaatcaa tccaatagtt aaatacactc gataattata tacgtctgta tttgaaataa
     6841 ccgaatacga atagctattt tcaaagtctt caaaaatatt agtggcataa tcagtgtgaa
     6901 atgcttgatt gaatcgatca gtgaatagtg aaatgacccg ttcataggct tctttttctc
     6961 tcgtcatcaa gaaaaattca ccttcaaaat cttttatttt gatcgattct tcaatggacg
     7021 ttacgcgaaa ggaggtatta gaatagggta ccttctcgtg agaagatttg acatcccctt
     7081 gataatacaa tgtcatttca agctttggct gtatcttgaa gttccaagat gtatctactt
     7141 cattggattc atttactgtt tttttcatat aagaaagctc agtttctgat gagacatttt
     7201 caaataccat gattagctgc tcatattctt cttgcccaat aaaatgttgt ggcaatcgaa
     7261 tcggagtata agtatctgtt tgatggaaaa attcatttgt ccgatagagt acctgtttat
     7321 ttatactaac caaggcaaaa ccactcatga taccggtcac tatgattgtg agtaaaactg
     7381 tgaacttttt tttgattatc accactctcc cttacttaaa tgattctttg ataaaatcat
     7441 accgtttttc atcaaaaaaa cgtatctatc aagtgtatag gtcatgtaga ataaatctat
     7501 atactgtaga atcgaaagga gaagaagatg aacaccggag aaaaaatacg aacttaccgc
     7561 agaaattgta agcttacgca aaaagaatta ggcgaaaaaa ttggtgtttc cgataaaacg
     7621 atttctagtt gggaaaattc tcgtacaatg ccagactttg aaatgctgtc acttttacat
     7681 catactttag gtctgccgat tgattttcct tctattgaat cagctaccga atcaatagaa
     7741 ccaacattgc cagatgaacc tcttgttaac aagaaatctt attcatcaaa acaacccttc
     7801 tcactcaaaa agacactatc tttctttctg ctcgtctttt gtttggtcta tctttttctt
     7861 atcaattatc aatacaaaaa tgaaacgatc gatcgattca atcctttttt gaaaccacaa
     7921 atcggctata ccacactccc tgtaggatac gtggacaatt ccatcgaacg ttgggtatgt
     7981 aatgatatct ttggccatgg tacatggctg tcttttcacg ctggagaggt agaaaaagag
     8041 aaaaaccatg ctgtaatcaa acacaaagga aaacacgtca tgaacgttcg cctgctcact
     8101 tgggaagcca ttccctcaga ttaccaagaa atcctcagcc aagactatga cgacgcctta
     8161 aaaagaagcc ctcaggagcc agataggcat taagtaggag atgaacgtag agatagaaaa
     8221 aatcaatgca catgatctta tccaaaaaac aggggtacaa attaattgaa aaagccttga
     8281 gatgaaagtt cctaaataaa aaagttcaga atgataggta tttctggctt atagtataat
     8341 atttacgcca cagccttcca atgattcttt ttggaatcat aatcaaggat ggacattcac
     8401 aaaaatattt tgctcattcg atccgaatag attaacgacc aatctagtaa gtaatataga
     8461 ttggtacgta gtcaagctgc acataattta gatcaatttt agttcttttt tatatttcta
     8521 tgatctgctc atttactttt atatgatggc ttacagtaac ataaataaat atgataatcg
     8581 ctggtagttt atccaagagt actcaataaa atacaaaatg gattgttcaa gcgcctatca
     8641 taatgataaa atgaaacaaa taaacgatac aaggaggaag agactttgag taatggcaga
     8701 acaatttttt cattgtaaaa attgttcagt tgttaatcta tgggatttcg gtagcatggg
     8761 cattttttta tagaggaatc acaccaacct ggccgtttta tctcttgtta gtgggaatat
     8821 tagtaggaaa ttttgcccct cttcactatc gtacgaactt ttttccaact gattctggcc
     8881 tttttcttaa aacaaagacg cgaaaattag aaaacattat tgaaatgaca attacctgta
     8941 taatcatttt cttagttata ttcctaaacg tcttctaata agaatctctt aaaattagct
     9001 tgttacagtt agttacctag tttatataac acagttatct tatcttcctt atcaacatct
     9061 tttgccaact ctttgtctta aattcgaaac atataagcag agctacgaac accaagactg
     9121 tttcggctct gttttttgca tcaaattcac tcttatatag ctgataatct cggctcttta
     9181 aaagaaatta ttttcgcttc attttattat aaaaaggcta ggaaccttga tgttatcagg
     9241 ttcctagcct ttatttaagt attaccccac agacccttcc atatagattt tcaaattaca
     9301 cactagttta agataaaaca aacaaggaaa aacttaccat ttactcaaaa aattaactca
     9361 tataagtaca ttagaatgta aaatagactg gtcaaaatct ggtcaaaaaa gtagatgtga
     9421 cacaagttcg cattcaaagc gatgatccat gatttcgtaa tggtcaatta tatggatcaa
     9481 agccacgcga caagagcgca atggatgtct ctaccgattg gttgaataaa cgaaataaat
     9541 agaatgtgaa aaaggatcgg acaaatgttc cagtttgctc gatccttttt tatttgaatt
     9601 cattttcgct ttatcaaaca tcatttgatt actcagttaa atacttttga catgagtacc
     9661 aatgaaacat ttattcatta aaattactta tttcttatcg cctttctagc caatgccaat
     9721 ccgccgatta ttccagcact atcacctaat tcgggcgtaa caatgtagct agcaattggt
     9781 ggtatttcaa catatccatt tataagctca acaaatttat cctgaacttt tttcttcatg
     9841 tgttcttgtt tcattacacc accgccaaaa ataattctat ctggcgaaag catcagtgtt
     9901 gtattgtatg cacattgggc aaggtaaaag gcttcaatct gccaaaacga atcgtcagcc
     9961 aataaattct ggccttttac acccaa
//
LOCUS       DAZUMK010000099         9954 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000099 DAZUMK010000000
VERSION     DAZUMK010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.101"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..439)
                     /locus_tag="ACG808_002212"
     CDS             complement(<1..439)
                     /locus_tag="ACG808_002212"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5047816.1"
                     /translation="MEEFGIDSISRTEALDFQADYEYELDQITMIPEIHQLLLALHKN
                     RIPIGILTNGPVDLQSRKLQNIGAYQYFEKQNIIISQSTHGGAAENSYEGNVKNLQSY
                     QNVQLSKRAIEILKEEIELNHQHIRFNPDYKDNGWIFTSKSRHK"
     gene            complement(662..1429)
                     /locus_tag="ACG808_002213"
     CDS             complement(662..1429)
                     /locus_tag="ACG808_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS5047817.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            1917..2342
                     /gene="lacA"
                     /locus_tag="ACG808_002214"
     CDS             1917..2342
                     /gene="lacA"
                     /locus_tag="ACG808_002214"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HFS5047818.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            2359..2874
                     /gene="lacB"
                     /locus_tag="ACG808_002215"
     CDS             2359..2874
                     /gene="lacB"
                     /locus_tag="ACG808_002215"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HFS5047819.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            2885..3817
                     /gene="lacC"
                     /locus_tag="ACG808_002216"
     CDS             2885..3817
                     /gene="lacC"
                     /locus_tag="ACG808_002216"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009024 - tagatose-6-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HFS5047820.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTVSHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            3820..4800
                     /gene="lacD"
                     /locus_tag="ACG808_002217"
     CDS             3820..4800
                     /gene="lacD"
                     /locus_tag="ACG808_002217"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFS5047821.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            4828..5145
                     /locus_tag="ACG808_002218"
     CDS             4828..5145
                     /locus_tag="ACG808_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS5047822.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            5145..6851
                     /locus_tag="ACG808_002219"
     CDS             5145..6851
                     /locus_tag="ACG808_002219"
                     /EC_number="2.7.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0019197 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase complex [Evidence IEA];
                     GO_function: GO:0015155 - lactose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactose-specific PTS transporter subunit EIIC"
                     /protein_id="HFS5047823.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTVLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            6993..8399
                     /gene="lacG"
                     /locus_tag="ACG808_002220"
     CDS             6993..8399
                     /gene="lacG"
                     /locus_tag="ACG808_002220"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFS5047824.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            8422..8694
                     /locus_tag="ACG808_002221"
     CDS             8422..8694
                     /locus_tag="ACG808_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HFS5047825.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            8715..9614
                     /locus_tag="ACG808_002222"
     CDS             8715..9614
                     /locus_tag="ACG808_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFS5047826.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         3138 a         1678 c         1996 g         3142 t
ORIGIN      
        1 gtttatgacg acttttggaa gtaaaaatcc atccattatc tttataatca ggattgaatc
       61 ttatatgttg gtgatttaac tcaatttcct ctttaagaat ttctatagcc cttttagata
      121 attggacatt ttggtaactt tgtaagtttt ttacattacc ttcatagcta ttttctgcag
      181 ctcctccgtg agtggactga ctaatgatga tattttgctt ttcaaaatat tgataggcac
      241 caatattttg gagttttcga ctctgtaagt caacagggcc attagttaga atacctattg
      301 ggatgcggtt tttatgtaga gccagtagta gttgatgtat ctcaggaatc atcgtaatct
      361 gatcgagttc atattcatag tcagcttgga agtctagagc ttctgtccta gaaatactat
      421 cgatgccaaa ttcttccata gtcttctgac agcgaaaaat tctaagctgc tcaatactga
      481 tgagtttttc gtgtatttag gaaaagcaac atcagaccaa catctgaaac gtttgtaaat
      541 gttatctatt tgttggatat caatcgttgg aaaacatttc tcaacgctac ttttaaaggg
      601 gagttgatgg tcataaagag tgtcatcaag gtcaaaaaca atatttttat aagtcataat
      661 tctagtccgc ttttaaaata gtagtgtact ggctaaattc ctctaagtgt tgagggctaa
      721 tctggttatc tgtaatgatg gtatcgagtt gatctagatt atagaagtta aagaaatcgt
      781 atcgatcgaa tttagtactg tctactaata agaatttttc tacagcattg tttagggcaa
      841 gttgttgaat cacaccttcc ttgtcactat atgtagcaat agaattatgg gtaacagcat
      901 ttgcactaac aaaagctttg gcaaatctaa ttgcttttaa atttgtcgaa gccattgaac
      961 ctacaaaagc tccagttatt tctctatatt caccgccaag aagcaataaa tcaatggttt
     1021 cgctatcatt tagaatcaag aacacaggga gactatttgt aatgacacga attttataac
     1081 ctttacgacc tttcaattct aatgccagtt gttcaagtgt agttccgggt ccaataaaaa
     1141 tagtatcgcc atctttgatt aaagagcagg cttttttagc aatgtcaatt ttttcttttg
     1201 tatttagact tttcttctcg atatgtgtct tttcaagtgg ctttttagag gaaagtaatt
     1261 gtgcaccacc atgagtacgt gttaaaagtc catcagcttc tagagcatca aggtctctac
     1321 gggctgtcat atcggaaata tctagttcat ctattatttc ttttatggtt atggtcccat
     1381 caatctttaa catatctaaa attttttcta atcgtcgttt tttgttcata tgaagaccct
     1441 tctttcataa agtaattttt ttccaaagat aattctcttt taattgtatc ataaaagaca
     1501 atattttcaa ggtaaaacaa acaatttcaa acaaaaacaa atgttagatg atgaaataag
     1561 aacagtggat tgacgtatat tagcttaggt cagattttgt ataagacgaa aataaagtag
     1621 gacctcttaa tcagtaagtt atagaaagta aaagactttt gtaatacttg aatagatatt
     1681 tcacgtccat tttgtgatgg attaaatgaa caaaaatgaa caataattta acggtgttat
     1741 ctatttttta aaaaaacaaa taaaaaaaca aaaaataaac aaaaaagtgt tgctttttgt
     1801 ttgaatgctt gatattatat aaacaaagaa atgatgaaaa cgttttttcg aacattttgc
     1861 aaaatgtttt ctacttctac gtagcatttc tttctaaaat ttaggaggtg gtcaaaatgg
     1921 ctattgttgt tggtgcagat ctcaaaggta cgagattaaa agatgttgtg aaaaattttc
     1981 ttgtagaaga aggttttgag gtaattgatg tgactaagga cggacaagat tttgttgatg
     2041 ttaccttggc agttgcctct gaagtaaata aagatgagca aaacttaggt attgtaattg
     2101 atgcttatgg agctggtcca ttcatggtag caactaaaat taaaggtatg gttgcagcag
     2161 aagtttcaga tgaacgctca gcttatatga cacgtggaca taacaattca cgaatgatta
     2221 cggttggcgc tgaaatcgtc ggtgatgagc ttgctaaaaa catcgctaaa ggtttcgtca
     2281 atggtaaata tgatggcgga cgtcatcaaa tccgagtaga tatgcttaac aaaatgtgtt
     2341 aaggaaagga gttaaggaat gagaatcgca attggatgtg accacattgt cacagatgaa
     2401 aaaatggcag tatcagaatt cttgaaatca aaaggacatg aggtccttga tttcgggaca
     2461 tatgaccacg cacgtactca ctaccctatc tatggtaaaa aagtcggtga agcagtagta
     2521 agtggtcaag cagacttggg agtatgtatc tgtggtacag gtgtcggcat taacaatgca
     2581 gtcaataaag ttccaggtgt tcgctcagcg ttggttcgtg atatgacatc agctatatat
     2641 gcaaaagaag agttaaatgc gaatgttatc ggtttcggtg gaaagattac tggtggtctt
     2701 cttatgaatg acatcattga agctttcatt gaagctgagt acaaaccaac agaagaaaat
     2761 aaaaaattga ttgcaaaaat cgaacacgtt gaaacacata atgcacatca agcagatgaa
     2821 gatttcttta cagaattcct tgaaagatgg gaccgtggtg actaccacga ttaaggtata
     2881 acaaatgatt ctgacagtca cactaaatcc ttcagtagac atctcttatc ctctagaaac
     2941 attgaaaatt gatacagtga atagggtgaa agatgttagt aagacagctg gtggaaaagg
     3001 cttaaatgtt actagagtct tgtatgaatc tggtgataaa gttactgcta caggtttctt
     3061 aggtggtaaa attggtgaat ttattgaaag cgaattagaa caatctcctg ttagtccagc
     3121 tttttataaa atctcaggaa acactagaaa ctgtattgct atcttacacg agggtaacca
     3181 aacagaaatt ttggaacaag gaccaacagt ttctcatgaa gaagcagaag gttttcttga
     3241 ccactatagc aatcttatta aacaatcaga agttgtgaca atttcaggaa gtttaccttc
     3301 aggacttccc aacgattatt atgaaaaact tatccaactt gcttcggatg aaggagtagc
     3361 agtcgttttg gattgttcag gtgcacctct tgaaacagtt ctaaaatcgt cggctaaacc
     3421 tactgccatc aaacctaata atgaggaact ttcacagttg cttggaaaag aagtaacaaa
     3481 agatattgaa gaactcaaag acgttcttaa agagtcactt tttagtggta tcgaatggat
     3541 agtggtctca ttaggtcgaa acggtgcctt tgcaaaacat ggtgatgttt tctataaggt
     3601 agatattcct gatatcccag ttgttaatcc agtcggatca ggggactcaa cggtagctgg
     3661 tattgcatca gctttaaata gtaaaaaaag tgacgctgac ttattaaaac atgcgatgac
     3721 attgggtatg ttaaatgctc aagaaacaat gacagggcat gtgaatatga ctaattacga
     3781 aacactaaac tcacaaattg gagtaaaaga ggtataaaaa tggtacttac agaacagaaa
     3841 cgcaaatctt tggaaaaaat ttcagataaa aacggtgtta tctcagcttt ggcatttgac
     3901 caacgtggtg ctttgaaacg tttgatggca cagtatcaag atacagaacc aactgtagct
     3961 caaatggaag aactaaaggt tttggttgct gatgaactta caaaatacgc ttcttcaatg
     4021 ttgcttgacc ctgaatatgg acttccagca acaaaagcat tggataaaga agcaggtctt
     4081 cttcttgcct atgaaaaaac aggttatgat acatcaagta caaaacgttt gcctgactgc
     4141 ttggatgttt ggtctgcaaa acgtatcaaa gagcaaggcg ctgatgccgt taagttcttg
     4201 ctttactatg atgtagacag ctcagatgaa cttaaccaac aaaaacaagc ttatattgaa
     4261 cgtgttggtt ctgaatgtgt cgctgaagat attcctttct tcttggaaat ccttgcttat
     4321 gatgaagaaa tctcagatgc aggttctgtt gaatatgcaa aagtgaaacc tcgcaaagtc
     4381 attgaagcga tgaaagtttt ctctgatcca cgctttaaca ttgatgttct taaagtagaa
     4441 gtaccagtta acgttaaata tgtcgaaggt tttgctgatg gtgaagtggt ttacagtaaa
     4501 gctgaagctg ctgacttctt taaagcacaa gaagaagcga ctaaccttcc atacatctac
     4561 ctaagtgcag gtgtatctgc taaattgttc caagaaacat tgcaatttgc tcacgactca
     4621 ggtgccaagt ttaatggtgt gctttgtgga cgtgctactt gggcaggatc tgtagaacct
     4681 tacatcaaag aaggtgaaaa agcagcacgt gaatggttgc gtactactgg attcgaaaat
     4741 attgatgaac ttaacaaagt tcttgttaaa acagctagtc catggactga taaagtatag
     4801 taaaaacata aaacggagga tattgttatg aacagagaag agatgactct cttagggttt
     4861 gaaattgttg cttatgctgg agatgctcgc tctaaacttt tagaagcgct taaagcggct
     4921 gaaaatggtg atttcgctaa ggcagatagt cttgtagtag aagcaggaag ctgtattgca
     4981 gaggctcaca attctcagac agctatgttg gctcgagaag cttctgggga ggaacttcca
     5041 tacagcgtta ccatgatgca tggtcaggac cacttaatga ctactgtctt attaaaagat
     5101 gtgattcatc acctcatcga actttataaa agaggagcaa aataatgcat aaactcattg
     5161 aacttattga gaaagggaaa ccgttctttg agaaaatctc tcgaaacatc tatcttcgtg
     5221 ctattcgtga tggatttatc gctggtatgc cagtcatcct tttctcatct atctttatcc
     5281 ttattgccta tgtcccaaat gcttggggat tccactggtc aaaagacatt gagaccttct
     5341 tgatgactcc ttatagctat tcgatgggta tcctagcatt ctttgttggt ggtactaccg
     5401 ctaaagcttt gacggactca atgaaccgtg acctacctgc gaccaatcag attaacttct
     5461 tatctacgat gctagcgtct atggtagggt tccttttgat ggcagctgag cctgcaaaag
     5521 aaggaggctt cttgacagcc ttcacgggaa caaaaggtct tttgacagca tttattgcag
     5581 cctttgtgac agttaatgtt tacaaggtct gtgtgaaaaa taatgttact attcgtatgc
     5641 ctgaagaggt tccaccaaat atctctcaag tatttaaaga cttgattcca ttcactgtag
     5701 cggttgttct cctttatgga tttgaactca ttgttaaggg aactcttggt gtaaccgttg
     5761 cagaatcaat cggtaccgtt cttgctcctc tcttctcagc tgcagatggt taccttggaa
     5821 ttacacttat ctttggtgct tatgccttct tctggtttgt tggtatccac ggtccttcaa
     5881 ttgtcgaacc agcaatcgct gctatcactt atgccaatat cgatgccaac ttgcatctta
     5941 tccaagctgg acaacatgca gataaagtta tcacttctgg tactcaaatg tttattgtta
     6001 ccatgggtgg aacaggagct acattgattg ttccattctt gttcatgtgg atttgtaaat
     6061 cagaacgtaa ccgtgccatc ggacgtgcct cagttgttcc aacattcttt ggggttaatg
     6121 agccaatctt atttggtgca ccaatcgtat tgaatccgat tttctttgta ccgttcattt
     6181 tcgctccgat tgtcaacgtt tggatcttta aattctttgt tgacacattg aacatgaact
     6241 cattctctgc caaccttcca tgggttactc ctggtccgtt gggaattgta ctcggtacta
     6301 acttccaagt tctatcattt atcttagccg gactcttggt agttgttgat actatcattt
     6361 attacccatt tgttaaggta tacgatgaac aaattcttga agaagaacgt tctggtaaaa
     6421 caaacgatgc tcttaaagaa aaagtagctg caaacttcaa tactgctaaa gcagatgccg
     6481 ttcttggaaa agcaggcgtt gctaaagaag atgtagcagc aaacaataat atcacaaaag
     6541 aaactaatgt ccttgttctt tgtgcaggtg gaggtacaag tggcttgctt gccaatgcct
     6601 tgaacaaagc agcagcagaa tacaatgttc cagtcaaagc ggcagcgggt agttacggtg
     6661 ctcaccgtga aatgttgcca gagtttgact tggttatctt ggctccacag gtcgcttcaa
     6721 acttcgatga tatgaaggct gaaacggata aattgggcat taaacttgca aaaactgaag
     6781 gagctcaata tatcaaattg acacgtgatg gacaaggtgc tcttgcattt gttcaacaac
     6841 agtttgatta ataaatttgt aaaagttatt tgagacgaga ttattctttc agttccttat
     6901 aatattgtta accatatcat tgcatttcat ttccttggtt tccggaactc agaatatcgt
     6961 ctcaaatgct ttttttgaaa ggatttatag ttatgactaa aacacttcct aaagatttta
     7021 tttttggtgg tgctacagct gcttatcaag cagaaggtgc gactcacacg gatggtaaag
     7081 gcccagttgc atgggataag tatcttgaag acaattattg gtacacagca gagcctgcaa
     7141 gtgattttta ccataaatat cctgttgatt tagaattagc tgaaaaatat ggcgttaatg
     7201 gtattcgtat ttcaattgcc tggtctcgta ttttcccaac tggctatggc gaagtaaatg
     7261 aaaaaggtgt tgaattctac cataatcttt ttgccgagtg ccacaaacgt catgttgagc
     7321 cattcgtgac tttgcaccac tttgacacac cagaagttct tcactcagat ggagatttct
     7381 taaaccgcga aaatatagaa cactttgtag attatgccgc tttctgtttt gaagagttcc
     7441 cagaagtaaa ctactggaca actttcaatg aaattggccc aattggagat ggtcaatact
     7501 tggttggtaa attccctcca ggcattcaat atgatcttgc caaagtcttc caatcacacc
     7561 acaatatgat ggtttctcat gcacgtgctg tgaaattgta taaagataaa ggttataaag
     7621 gcgaaattgg tgtagtacat gcgttgccaa ccaaataccc ttatgatcca gaaaatccag
     7681 cagatgttcg tgccgctgag ttagaagata tcatccataa caaatttatc ttggatgcaa
     7741 cctaccttgg acactattca gataaaacga tggaaggggt caaccacatc ctagctgaaa
     7801 atggtggaga acttgatctt cgtgatgaag acttccaagc acttgaagca gctaaagatt
     7861 tgaacgattt ccttggtatc aactactaca tgagtgattg gatgcaagcc tttgatggtg
     7921 agactgaaat cattcacaat ggtaagggtg aaaaaggaag ttctaagtac cagattaaag
     7981 gtgttggtcg ccgaatcgca ccagattatg tgcctagaac ggattgggac tggattattt
     8041 atcctgaagg cttgtatgac caaatcatgc gagtgaaaaa tgattatcca aactacaaga
     8101 agatttacat cactgaaaac ggtcttggat acaaagatga gtttgtagat aataccgttt
     8161 atgatgatgg tcgtattgat tacgtgaaga aacacttgga agttttatca gacgcaattg
     8221 cggatggtgc aaatgttaaa ggttacttca tctggtcact tatggatgtc ttctcatggt
     8281 caaacggata cgaaaaacgt tatggactat tctatgtaga ctttgacacg caagaacgct
     8341 atcctaagaa atcagctcat tggtataaga aagtagcaga aactcaagtg atagagtaat
     8401 attacttgaa aatgtaaaaa attgttatcc gatactttag aaaaagtaca acacttattt
     8461 agaccaggct ttgaaaaggt atatattgtt agtttcgaag attgtcctat gattcctgag
     8521 cttgaagcta ccccgttgct aaaatgtggt aaatggtatg tttcaactgg aaaagagtgg
     8581 atttgccatt ctgatttaga gttatcagca tttgaatggg aatttttaca atcactcgat
     8641 gtagaaatac gtgagactat tcacttcgag gtaaattatt tgcctttcca ataataaaaa
     8701 gaaaggaatt agttgtgact atcgaattaa aaaacgagta tcttactgtt cagtttaaaa
     8761 cacttggtgg tcaactgact tcgattaaag acaaggatgg gatagagtat ctctggcaag
     8821 ctgatccaaa ttattggaat ggacaggcgc ctattctatt tcctatctgt ggtagtttac
     8881 gaaatgattg ggctatttat agacctcaag aaagaccttt ttttacaggt cttattcgta
     8941 gacatggttt tgttcgaaaa gaagaattta ttctggaaga agtcaatgat aatagcgtga
     9001 cctttagtat taaaccaaat gctgagatgc ttgataatta cctatatcag tttgaactga
     9061 gagttgttta taccttgaat ggtaaatcaa ttagaaccga atttcaggta acgaacttgg
     9121 aaatggaaaa aacaatgcct tactttattg gagcccatcc agcattcaat agtcctttag
     9181 tagaaggtga aaactatgag gattattata ttgaatttag tgaggttgag tcttgctcga
     9241 taccaaagag ttttcctgag acaggattat tagatttgca agatcggaca ccttttttgg
     9301 aaaatcaaaa aattctcgat ttggactact cacttttttc ccatgatgcc attacacttg
     9361 accagctcaa gtctcgtagc gtaacgcttc ggtcaaggaa gtctggtaaa ggtcttcgag
     9421 tagattttga tgattttccg aacttgattc tgtggtctac aactaataaa agtccattca
     9481 ttgctttgga accttggagt ggtctctcca cttcacttga agaaggtaat ttcttagaag
     9541 ataaaagaca ggtaacgaaa atttcacctc aagaaacttc tagaaaaatc tatgatatta
     9601 ctatccttaa ttaaaatttg ttattatttg agtcttcaat aagatttgaa tactcttacc
     9661 ttgtgatgat agattggtct tctccgtgcc agtcttacat cgcaaggttt ttaagttttt
     9721 tgtgggtcta tgtcaactgt agtgggtgac gaaaagctaa tatctaaaga cagtctggta
     9781 gtaaaaaata agattattta ctacttcttg atttaaaata atagtggagc cattaatttg
     9841 tggcaaattt aatttaatat tttgcctcaa attagccaca ctttttatag aactaagagg
     9901 aaaaatgaaa ttttcttcct attaatatag ttgtttgaaa tcttaaatat agcg
//
LOCUS       DAZUMK010000100         9881 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000100 DAZUMK010000000
VERSION     DAZUMK010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.102"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(42..554)
                     /locus_tag="ACG808_002223"
     CDS             complement(42..554)
                     /locus_tag="ACG808_002223"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFS5047827.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(568..2865)
                     /gene="recJ"
                     /locus_tag="ACG808_002224"
     CDS             complement(568..2865)
                     /gene="recJ"
                     /locus_tag="ACG808_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFS5047828.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLMHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPVE
                     FLDLVAIGTIADLVSLTDENRILVSLGINAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSN
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     IMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3007..3450)
                     /locus_tag="ACG808_002225"
     CDS             complement(3007..3450)
                     /locus_tag="ACG808_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFS5047829.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3451..4239)
                     /locus_tag="ACG808_002226"
     CDS             complement(3451..4239)
                     /locus_tag="ACG808_002226"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS5047830.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETDFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4236..5177)
                     /gene="rnz"
                     /locus_tag="ACG808_002227"
     CDS             complement(4236..5177)
                     /gene="rnz"
                     /locus_tag="ACG808_002227"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFS5047831.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5354..5941)
                     /locus_tag="ACG808_002228"
     CDS             complement(5354..5941)
                     /locus_tag="ACG808_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFS5047832.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6162..7475)
                     /gene="obgE"
                     /locus_tag="ACG808_002229"
     CDS             complement(6162..7475)
                     /gene="obgE"
                     /locus_tag="ACG808_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFS5047833.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7763..8773
                     /gene="gap"
                     /locus_tag="ACG808_002230"
     CDS             7763..8773
                     /gene="gap"
                     /locus_tag="ACG808_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFS5047834.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(8858..9793)
                     /locus_tag="ACG808_002231"
     CDS             complement(8858..9793)
                     /locus_tag="ACG808_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5047835.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2715 a         2268 c         1708 g         3190 t
ORIGIN      
        1 cttctttaag aataacatca agaatagctt cttttttctg cttaatattc cattaatgta
       61 atgatgtcat atcctttgat tttgtcgcgt ccatggagat ccatcaattc gatcaaaaat
      121 gcgcagccta cgactacacc gcctaattgt tcgatcagtt cgatcgtcgc tttgattgtt
      181 cctccagtag ctagcaaatc gtcacaaatc agcacacgtt gacctggttt aatggcatcc
      241 ttatgcaatg tcaatgtatc tgttccatat tctaatccat aagtcacttc gatggtttct
      301 cgaggtaact tccctttttt gcggactggg gcaaaaccga ctcctaactc ataagcaaca
      361 ggacatccta cgatgaatcc acgggcttct ggcccaacta ccatatcaat tcgtttttct
      421 tttgcataat ttacgatttg cttcgtagct tcccgataag cgtcaccatc tgccatcagt
      481 ggggaaatat ctcgaaacac aattcctttt gacggatagt cgggaatact ggcaatataa
      541 tcttttaaat ccactctttg tttcctccta ggttgttaac catttcttta tggtaggaat
      601 atcgctcaaa agcaaaaatt cctcaacttt gattttattg agccgccgct ggtataatct
      661 gctctcagtc aaaggatgac tttctgggct agcgactttt tgtaaaacac cgttctctat
      721 tgtaacaaat ttcaattcaa aaaacacctg tatcataaaa attaataatt tttcagggat
      781 acgcaaatat tgagctattg cttttatttt atgacgtata tcaattcgtt cttgagaatg
      841 gatcagttta aacaattttg cgtactgttc tcttgtgcct accccattca aataggcctc
      901 atcaggcgaa atccccatca gataaacacg attgaatgaa taagaccgaa aaacttcttt
      961 cagatccgct aaatcatctg gacaatccat aataactaga gagttcggtt ttcttttttc
     1021 aattattgcc tttacagaat ctagtccttc gaaaacaatc gcagcttcct ggataatctc
     1081 aggcacgaat ttcaacgtag aagaatcaaa tgctaaataa aggctacctt ccgacgttcg
     1141 gctctgttca cggaaacgtt ttgcccgcca atcaaatact tgcagaccac ccacagaaaa
     1201 atcagatacc atcagctgcg gtttcttacg cccgttccat tcattgatcg aaagctttcc
     1261 taccacatct acctgattat tcagcagttc ttcttcttga gtaccaaaac caaaagcgac
     1321 cgcatcaagc tgactcgatg cattggaaag gacaaatttc agatgctgtt ggtttgctcc
     1381 gattcttttg atattttctg cttggacttg tcgaaataaa aactgaggca aaggattatc
     1441 cgtaccaaat ggcgccaata gtttcagctc atcaattcgc tctactgtta cttcatttgg
     1501 cagcaatact tcatcaattc tcaactcagg ccctctcatc aagtcaattt gatgatcgac
     1561 tatatattgg ttcatcttct cctgtagtat agtgacattt tcacttggca tcgttaaacc
     1621 gactgctgca tgatgtcctc cgaagtaagt aaataaatct cgcatctggt caagcatttc
     1681 aaaaagattc aaggcttcaa tactacgtcc agatccttta gctgatccat cttcttttaa
     1741 agtaaggaca agcgttggtt tacccgtttc attcattatt ttcccagcaa caatgcccaa
     1801 aacgccttca tgccatcctc ggtttaccaa tagatggata tgattctcct tattgaccat
     1861 cgctaatgct tcttgtgtta tctgctctac gattgctttt ctttcttcat tgatctcatt
     1921 caattttttt gcttgaacgg atgcttcctc ttcatcaaac gtcgcaagta acgagacggc
     1981 aggacgtgga tcgcccatcc gaccgattgc atttagtctg ggagcaatcg aaaaaccgat
     2041 cgttgtttcg tctgcgtccc tcatttttac gccgctttct tcaaatagcg cctgaagtcc
     2101 tatccgttct gaatgatgaa tggcattgat tcctaatgaa acaaggattc gattctcatc
     2161 agtcaatgaa actaaatcgg cgatcgttcc tatcgctact aaatccaaaa attctacagg
     2221 cggttcttcc aataatgcag aagctacttt gaatgctact ccgactcctg ccaaatctgg
     2281 aaatggatag tgccccgctg gatgacgagg gtgaacaatc gcataagcgt ctggtaaatt
     2341 ctcaggtagt tcgtgatgat ctgtaatgat cacgtctaca ccagcttgct gagcgtatgc
     2401 taccgcatca tttcctgcta ccccgttatc aacagtgatg atcaactgga ttccttcttc
     2461 gatcttttct tggtatacag attggtttgg tccataccca tgctcaaatc gatttggcag
     2521 gaatacttct acttctgcac caagtaattc cagtgtttct ttcattaccg ttgcactggt
     2581 gatcccatct gcgtcataat caccgtaaac gaggattttt tctccactga ttacagcttc
     2641 ttggattcgg ctgattgctt tatccatatc gtgcatcaga tacggatcat gtaattcttg
     2701 ttcctttgga tgtaaaaatc ggttgatatc cttttcctca tggtaacctc tttgccacag
     2761 taacttaccg acaaatggag ataatttcag ttcacggatc atttggagaa aggattcaga
     2821 aggctctgtc tcgcttaact tccaatcaaa atttgcttgt ctcactttct cactcctaac
     2881 ttagttatta tagcaaagga aatcagcaaa aaaaagcaga aagaaagaat aaaaacagat
     2941 cagatccgct agacagatgt tcagcaggac ctgatctttt gtacgaccat ttatgaaata
     3001 tccctattag tcaaaataat tgaagctttg tttgggcgat tgagtttttt gttcttccgg
     3061 cgctttcaat tgactgattt gtgcggtttt cgcttgtaac tccgcctgat aagcagcttc
     3121 caactccgca cgttcgtttc taaactcgcg ttgctgttct tcttttgctt cttcaatttt
     3181 cttttgaagt tctgtttgat attctgacat ttcctgagtc agctgtttga tttgcttttt
     3241 ttgctgccat agtgttgccg aagcagtcaa aaatacaatc agtgcaccaa tcagcgcaga
     3301 accgatgatc actaagatca atggcgcaga aaaggaagtg aagccaaaac ttacaggaac
     3361 atttttattg ttcaatacag caaaaagaac aataatcaaa actaaaacaa atccaagaat
     3421 cacattgctt tgctttttca attccttcac ctactttttg ttaaaaattc cgctggctaa
     3481 ataatcacca atcgttggaa acagcgtata aaattttgat gcaacttcta aaataaacgg
     3541 ttgattgatt tcccgttttg gatgtctcat attgcggacg atcatttttg ccaacttggc
     3601 aggttctaaa acgatttgtc cgactttttc caaatagctt ccgcttggat cagccttatc
     3661 gaagaaatct gtctcgattg gacctggatt cactgtcgta acagctacgc caagtggttt
     3721 caattctaat cgcaaagcgt tggagaagcc aaggactgca aatttcgtgg cagaataaac
     3781 cgttgatttt gcagttgcca tttttccagc catagacgcg atattaatga tatgaccttg
     3841 tcttctttct gccatatcga tcgcaaattt ttgtgtcaaa accatcattc ctaagatgtt
     3901 gacttcaaac atatcgtatg cttttccaag gtcgaatgta aggaagtctt cgaaaatccc
     3961 aaaacccgca ttgtttacca gaatatcgac gcgcccgact tcttccatca ccttttcata
     4021 taattcgtcc acgctttcag gattgctgac gtctaccttg aaagcataag cctctttgcc
     4081 gctcaagcgt ctgcactcgt ctctgacacc ttctacaaat gccattcttc gtgcgcaact
     4141 tataacgatt gctccttgct ttgctgcttc ataacagatt tgtgcgccca gaccgcctga
     4201 actacctgtt actaaaacaa ctttatcttc taaattcatg cctcttcctc ctcgcgaaat
     4261 ggaatctcaa tgctatcaaa atctttcatg atcgtcgtgt tttcaaacac ttcttgtgct
     4321 tctttttcaa gctctaaagc tgcttttcct aaatatctcg cactgatatg agtaagtagc
     4381 agccgttttg cctgcgcttc ttttgcaact tcagcagctt gatgagtcgt ggaatggaaa
     4441 tagttgtgcg ccattcttgc ttcatcttta ttaaatgtgc tttcatgtac cagaatatcg
     4501 ctattttccg caagcacaac ggaattatgt gtttttctcg tatcgcctaa aatagtcacg
     4561 attcgtcctt ttttatctgg acctacgaaa tcttttccat tgatcgtccg cccatcttct
     4621 agttggatcg tttcaccttg cttcagtttg ccgtacagtg gtccagctgg aatacctaat
     4681 tcttttagac gatccacctg caattctcct ttatgatcat gttcaaccac acgataacca
     4741 aaactgtcga tcccatgatt tagttttttt gtataaacag aaaattgctg atcagtaaaa
     4801 atcggggctg tttcatttag ctcaatgaat ttcagcggat aacttaaacg cgtttgcgaa
     4861 atacctaaag agacttttat gtattcctct atcccttttg gcccgtaaat ctctaatggc
     4921 gtatccccac cttgaaatga ccggctgctg atgagcccgg gaagtccaaa aatatgatcg
     4981 ccgtgtaaat gtgtaataaa aattttaccg atttttctcg gacgaatagt cgttcgcaaa
     5041 atctgcattt gcgtgccttc gccacagtcg aacaaccaaa cttcattgcg ctcgtctaaa
     5101 agtttcaatg ctatacttgt cacatttcta tgttttgcag gaacacctgc acctgttcct
     5161 aaaaattgta attccataat ctacctgact ttctttaagt tcctctttaa ttttagggta
     5221 aaaagaaggg ataatcaacc gtaagtcgag aatgggttca aattaaaaat aacgttctaa
     5281 agcgatagcg ttttctttga aaattactat aaaaacagca gcgaacccgt ttgagatcct
     5341 ctgctgtttt ttattaattt gaagatccag aactttcatt gtcgttggaa gtttgcggat
     5401 gtttgtcctt ccaagtcttt tgtgcagctt ctttcgcatg attttcagct tctttcgcat
     5461 catgcacatc tttttcgaca tcttcataat ccaagcgggt aatttctccg cctaattcca
     5521 tcattaccaa ttgattcagt gggcgattgt cctcttcatc agcaatcaag atcagtcctg
     5581 tagctccttc accgatttta tttcctacaa agtcgaacac ggtctgggca tcttttattt
     5641 ctttggcatc ttgtgtcgca ccgatcattc ctccggcaaa ccatcctaat aaaatcccta
     5701 gcggaccacc taatacacca accaacattc cgattaatcc acccgtagaa gatttgttat
     5761 taccggtaaa gtcgataaaa tcttctattt tgaattgatg tgtcccatta ggatcatggc
     5821 tgacaactgc catctgttct cctttaatcg aacgaacagc atgtaacttt ttgatttctg
     5881 aaaatgcctg atatgcctgg ctttctactt gaaaatttaa aatgattact cgtttgctca
     5941 ttaaaaacgc ccctttcata ccttcttgtt atttatttta cctttaattg taaaataaga
     6001 aaagaaaagc actctttttt tccaataaaa tagggctgtg acaaaagtcg tatcacagct
     6061 cctttttccg aataaacggc gttcttgaag ctgatcactt ctgtcacaac ctcccttaaa
     6121 tctgaatcta tattttttgg tatttgtttt tatttgcttg cctattcgac aaattcaaat
     6181 tcaaactcac cgatacgtac aaggtcgcca tctttcgcac cgcgtgcccg aagagcttca
     6241 tccacaccca taccacgtaa ttgacgagca aaacgcatca ctgtttcatc atgatcgaaa
     6301 ttcgtcattt ggaataattt ttcgatcttc tcaccagata agatccacgt tgcatctgaa
     6361 tcacgatcga tttggaactc aggaccttct ggattgaatc catagtgtac agtttcttct
     6421 tccagttctt cttcgtacaa tgggaattct ggcgttactt ctaacaaatc tgctgtagca
     6481 ttcaataacg cgtccaatcc ttgtcgagtc actccagaaa ttggaaatac tgggatgtca
     6541 tcagcaaatt catcttcttt ttccttgttc agctgttctt tgaatttcac tagattttcc
     6601 ggtgcatccg gcatatccat tttgtttgcc acgatgattt gcggacgctc tagcaagcgt
     6661 aagttataag tagataattc tttgttgatg gcaagatagt cttcataagg atctcgtcct
     6721 tccatcccac tcatatcgat cacatgtaaa attactcgtg ttctttcgat atgacgaagg
     6781 aattgtgtac ccagcccaac accttgcgaa gctccttcaa tcaagcctgg caagtctgct
     6841 gcggcaaaac ttctgccatc acttgtagta accattccta gattagggac gagcgtcgtg
     6901 aaatgataag cgccgatttt tggacgagca gatgaaataa cggatagtaa agtcgatttc
     6961 ccgacagatg gaaaacctac aagtccaacg tctgccaata ctttcaattc tagttcgatt
     7021 tttcgctctt gtcctggttc gccgttttca gcaatttccg gtgcaggatt ccgaggagag
     7081 gcaaaacgga tattccctcg tccaccgcgt ccaccttttg ccacaactag tgtttgacca
     7141 ttttcaatta aatctcctaa aagagcacca gtctccgcat ctcggacagt ggttccttgt
     7201 ggtactttaa cataggtatc ttctgaacca cgaccgtgca tccctttact catcccattt
     7261 tcacctggct gcgctttgaa atggcgattg aaccgaaaat ccatcaatgt acgtaaccct
     7321 tcatcgacga tcaagatgac atcgccgcca cgaccaccgt ctccgccagc cggtccgccg
     7381 tcaggaacgt atttttcgcg acgaaatgca accattccgt ctccgccctt gccagctttc
     7441 acatcgatcg ttacttgatc taaaaacatg gacataatta agtcctccta ttattcattt
     7501 atttatttga ctgcctgaat ttccacaata aacaaaagtc ttacttattt tatcggtgaa
     7561 atctgatttt gtctatcatt tcagcaaaaa gattgtttac cttcttcttt ttttatcatt
     7621 tggtaaggtt tccaaccttt tttctgctac caagtataat tttttgacaa aatattcttt
     7681 gcgtagcaga ataacagtgt attaattgta ttaattgagg atgccgtttc acagcacccc
     7741 taataaagga ggaacttttg atatgacagt aaaagtaggt attaacggat ttgggcgtat
     7801 tggccgtctg gctttccgcc gcatcaaaga agtctcagat gatattgaag tagtagcaat
     7861 caacgactta acaagtccaa ctatgttagc acaattattg caatttgact ctactcatgg
     7921 tacttatcct ggcacagtta ctgcaacaga agactcaatc gtagtggatg gcgaagcaac
     7981 tcgtgtttat gcagaacccg acgcaagcaa aatcccttgg gcaaaagaaa acggtgtaga
     8041 tatcgtacta gaatgtactg gtttttatac atctgaagaa aaagcaaaag cgcatttaga
     8101 tgctggtgtc aaacgagtag ttatttctgc cccagctggt gctatgaaaa ccatcgtcta
     8161 taacgtcaat gatgatacat tagatgaaaa cgacaaaatc atctcagcag gttcatgtac
     8221 aacaaactgt ttagcaccta tggcttattt cttaaataat gaatttggga tcgaagttgg
     8281 tacaatgaca acagttcatg cctacacttc cacacaaatg ctcttagatg gacctgtaaa
     8341 aggcggtaat ttgcgtgctg ctcgttcagc tgctgacaat actatcccac attctactgg
     8401 tgctgcgaaa gcaatcggct tagttatccc tgagctacaa ggaaaattac aaggacacgc
     8461 acaacgtgtt ccagtcgttg atggttcatt aactgaattg gtttcagtat tgaaaacaaa
     8521 agtaactgct gatcaagtaa acgaagcaat caaaaaacac acagttgaca atccttcatt
     8581 cgggtatgat gaccgtcaaa tcgtttctag cgatgtcatt ggaacaactc aaggttcgat
     8641 ctttgaccca acgcaaacag aagtaacaac tgctggtgat ttccaacttg ttaaaactgt
     8701 tgcatggtat gacaatgagt atggatttac ttgccaaatg attcgcttac tagaaaaatt
     8761 cgctaacttg taatttgata gatcaatcga taaaagaagc ctttgactgg atccagtcaa
     8821 aggcttcttt cttttcatcc cctactttta aactcgttca atgaacatat tggcttaaaa
     8881 attgttggac tacttgctga tatttttcag gatctgtctt gtatgcttcc gcatgctcgg
     8941 ctccttttac aacatacttt tcttttggtc ccttcgttgc acgatagacc ttatctaaca
     9001 tttcaaatgg tacaaatgta tcttcgtctc catgtataaa gagcattggg cgctggtttt
     9061 tcttcaattg ttccactgca tttgcttcac cgaaaaagta gccagcacgg acttttgtca
     9121 ctagactcgt tacttgcata agcggaaatg ctggaagact aaacatatcg tctagctgat
     9181 aagcaagctc atcatgaacg gaagaataac cgcaatcttc aatgattgct ttcacattat
     9241 ctggtagttt ttctccgcta gtcatcataa ctgttgctgc tcccatgctg acaccgtata
     9301 gaacgatttc ttgcgatttt ccattgtttt ctagcacttt gtttatccac tgtacatagt
     9361 ctttacgctc tggccatcca aatccaatgt aatccccttc gctttttcca tgtcctcttg
     9421 catcaggaac aaggacattg taacccatat catgaaacat ctttgcgtaa ttggtcatcg
     9481 tttcagcgtt tcccatatag ccatgagcca caagagctgt tttttcactt ttcgtttcag
     9541 caggaagata aatcgctgat aatttcagac cgtcttttga agtgatggtc caatcgcttc
     9601 ggttttcttt tgaactaaac catttatgtg catccgtttc tttggatgtc tgtgttgtac
     9661 caggtgtatc ttcgtctaaa aagtctttct cagaaggcac aacagcataa ttataaaaat
     9721 aattccctgc aaacactaac gcaacaatca gagctaaagc gacgcctaaa aggattttca
     9781 tccaaagttt cataaatcat ccatctcatt tctctttttt catataacct tgaattagta
     9841 taatcaaaga atctctacta tgcaatgttc atttttataa c
//
LOCUS       DAZUMK010000101         9437 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000101 DAZUMK010000000
VERSION     DAZUMK010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.103"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(22..1950)
                     /locus_tag="ACG808_002232"
     CDS             complement(22..1950)
                     /locus_tag="ACG808_002232"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS5047836.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(2147..2980)
                     /locus_tag="ACG808_002233"
     CDS             complement(2147..2980)
                     /locus_tag="ACG808_002233"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFS5047837.1"
                     /translation="MFFANIIKAVILGIIEGITEWVPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(3172..3876)
                     /locus_tag="ACG808_002234"
     CDS             complement(3172..3876)
                     /locus_tag="ACG808_002234"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS5047838.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(3899..4525)
                     /locus_tag="ACG808_002235"
     CDS             complement(3899..4525)
                     /locus_tag="ACG808_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS5047839.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            4851..7226
                     /locus_tag="ACG808_002236"
                     /pseudo
     CDS             4851..7226
                     /locus_tag="ACG808_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
     gene            complement(7250..7741)
                     /locus_tag="ACG808_002237"
     CDS             complement(7250..7741)
                     /locus_tag="ACG808_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFS5047840.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            7928..8848
                     /locus_tag="ACG808_002238"
     CDS             7928..8848
                     /locus_tag="ACG808_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS5047841.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            9002..9346
                     /locus_tag="ACG808_002239"
     CDS             9002..9346
                     /locus_tag="ACG808_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFS5047842.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
BASE COUNT         3041 a         1722 c         1810 g         2864 t
ORIGIN      
        1 aatcataaaa aaacactttg cttatttgac agaagacagc cgcgctttgt atagtggaca
       61 agacttacat ttttttgaag aatcagaaag atcaggtaaa ggtcttagta aacgcaagcc
      121 attcagaata accaatattg tgcttccttc gtgtcctaca actccaagag gtagatttaa
      181 aaattgcagg aagttagaag caatcaacaa gagaataaca agaatcgaaa aaataatatt
      241 ttgtttgatg atccgccgca tttttaaaga tagccgatga ctgtagacaa atttatttaa
      301 gtcgcttttc attaagacga tgtctgcgat atccatagcg gcatctgtcc cttgccccat
      361 agcgacacct aaagaagcgg ctgctaaagc aggcgcatca ttgactccat caccaaccat
      421 cgcatttact tggtaaagtt cattctcttt tttgactagt tgtgtttttt cttccggagt
      481 acagcttgca tagtaatgtt caatccctac ttgttcagca atggcctttg ctgtgccaga
      541 atggtcgccg gttatcatac ttgtatggat gttttgggat ttaaagtagg aaattacttg
      601 ttgggtattt gcttttggaa tatctagcag acctaaaaca gcaattaact gctgatcttt
      661 agaaagataa atcacggttt tgccttgatt ttccaaacgt tcgatcattt ccgcaatctc
      721 tttgctgatt atcatcgtct caggatcata tgcgtgcttg ccaactttcc attgcgcacc
      781 taaataggta gtttgtaatc caaaaccagt aatttcttct attggtaagt attggatttc
      841 ttcaggtatt gtgcaatcga aatattgcaa aatcgcatga gcaagtggat gcgtcgtttt
      901 ttgttccata gcaacaagaa gttgttgtgc caaatccttt tcatcaaaga aaaagctgtc
      961 agttacaact ggttttcctt tagtgagcgt tcctgtttta tcaaaggcaa tcgcttttaa
     1021 ttcagaaagc ttttctaaat ggataccgcc tttgattaaa atcccatttt ttgcagaatt
     1081 tgaaagagca gctagagaag ctggtgtagc agatgcgaca agcgcacaag gagaagccac
     1141 aactaataat accattcctc gataaaagct ttcatcccaa ctccaaccta ggaaaaagtg
     1201 ggggagaagg atcattagag gaacagcaag caaaatcaat tttacataat tattttcgat
     1261 tcgttcgata aatgaagctg tttgagttgg cgtattctgc gcttcttcta ctaattggat
     1321 aatcttagca aagacagttt cggaactcgt ttttgttact cgaatcgtga tgggatttcc
     1381 ttgattcagc gttcctgcat acatctcatc gcctaaatat ttttctacag gcacagattc
     1441 accgcttaat gcagactcat cgatcgttgc ctgtgtgctt tctaaaaagc catctatcgg
     1501 aacagctgct cctttagcga caaataccaa gttatccacc tttagttgag aaacatctac
     1561 ttcttgcatt tgtccatttt cttttaataa ttgggctttt tgtggttgaa gattcatcaa
     1621 agcagtaatt tctttttgac ttttattagt tgtgtattcc tctaatgcac cgcttaaaca
     1681 aaagatgaat gtcaacattg ctccttcaaa gtaattaccg atcaaacagg caccaatagc
     1741 agctaaagcc atcaataaat ccacattcag tgtccgatca ttccacaatt ctttcaagcc
     1801 ttccgatgtt tgtttatatc caccgatgat aatggcactg atataaaaaa gtggggtgat
     1861 ggtaagagag aatcggtcga agataaaacc gaagatcata aataaaaggc agccgattgt
     1921 tgctgcaaaa gcttttttct gattagtcat cgttattaga cctccttgtc aaaacaagga
     1981 tagcataata aaaatgtaat tgataataat aacagctaaa tgagaacgat aatggttatc
     2041 atttggtgat taagcttgat gttgttttta ttatttataa tgattattat taaagaaaat
     2101 aaaaaaccag aacatttctc aaataagaga agtgttctgg ttttttctac attgcaataa
     2161 accaataacc aatcaaaatg ataccaagga taatacgata ccagccaaaa gcggtaaagt
     2221 cgtttttctt caaatagctt agtaagaatt tgatagctaa gacggaaacc acaaaagata
     2281 cgaatgttcc tacaagaaga acaacagttt cttccatttt gaatgaattc ccttcagcta
     2341 aatatttgac gatcttcaag ccgcttgcgc caaacatgac aggaatccct aagaaaaatg
     2401 aaaactctgt agcgacaaaa cgcgaagcac caatcaagat agatcctagg atcgttgcgc
     2461 cagaacggga tgttccggga acaagtgcca agacttggaa taacccaaca agaagagctg
     2521 cttggtacgt aaagtcattc agagaagtcc aacgaggtgt tttgtttttg ttgcgttttt
     2581 cgacaataat aaaggcaata ccgtaaacga tcaaaacaat ggcaactggt aagaacttgt
     2641 ggaaatttgc ttctaaccaa tcatccaaag gcaaaccaat gatgatggct ggtatgcaag
     2701 caacgatcac tttaaaccaa agtgtccacg tatctttttt ttctaccgtt gatttacttg
     2761 gagaaaatgg gtttaacttg tgaaaataaa gaacgacgac agccaagatc gcccctaatt
     2821 ggataacgac attgaacatc gacataaatt gtgaactggc atccagtttg atgaattcat
     2881 ctgctaggat caaatgacca gtactactga tgggcaccca ttccgttatt ccttcaataa
     2941 taccgagaat cactgctttg ataatatttg caaaaaacat aaattccttc cttcccaatt
     3001 aagatacatc ttatagtata ccgttatttt ttgagaaacc caatctgttt tttaatatta
     3061 agaaaaactt gagaaataaa gcagataagt acacgaagag aaaaaagaag acaaagccct
     3121 cgttcagact ttgcctttta tgattctctg gtaaaaatta aaatatcccc attatagaaa
     3181 cgagtcgcga ctatgtgttt tcccgttatc gatataagac gtatcattga tgccgtggat
     3241 agaaaatcga ccgtttgcat aagtgacctt tgttacgctg ccatttaaaa gcccagcacg
     3301 aacaggctgt ttaggatcga tcagattaag caaaaaggcg atcgtcaaac catgagatac
     3361 gactaaaaca tttccaccac catttttttc gcagtgatgg gcgatatctt caaatcctgt
     3421 ccatacacgg tctcgcaatg tttcataagt ttccgcccac ccagcggtat ctaagttgta
     3481 tacagcatta gcaatttgtt caaaagaaac ttctgtggta aacatttcat catatgtttt
     3541 aaaattcaat acacgaggga tcacacccca catttccatg tcatatccac cttcaagaga
     3601 cccgaaacac cattctctaa tccttttttc aataaaataa ggaatttctt ttcccttaga
     3661 atgttctcct agaataatac gggcagtttc gattgcacga ccactgtcac tagaataagc
     3721 aaatgaaaag tcgacatttt ttaacccgcg acctaaataa tgaatgcctt cttgtccttg
     3781 ctttgtgagg ggtgtatcac accagccttg tactcgttca attgtattga acatcgtttt
     3841 tccgtgtcgt acgatataaa gtgtagtttc tgtcatttta ttctcctcct aagtcgtttt
     3901 aacgattgaa aaaaggattt gtcctttttt cctgttcaat cgtcgttgca tcgccatgtc
     3961 ctggatagac tgcaaattca tcaggtaata caaacagctg ggtccgaata ctatgaagta
     4021 attgttctaa attccctgta tacagatcgg ttctaccgat acttcctcta aacagggcgt
     4081 cgccagaaac gacaaaatca tcaaaaataa agctgacgct tcctatagaa tgccctgggg
     4141 tagggacgac gcgaaaagac atgtttccta aagtataagt tttcatttca aattcatatt
     4201 ctgcaggcga gacaataaca tcagcaatat catcatgtct accgagtcca gaaagattca
     4261 aaatcggatc acctagccat ttttgctcta gtgggctgac ataaactggg atttgataat
     4321 acgcgcgtaa aggttccact gcaccgatat gatcgtagtg agtatgggta agcaaaatcg
     4381 ctaccggctg ttggtttgtt tttttgattt cttgtttgat tcgttccgca tcttctccag
     4441 ggtcaatgat caaaagtgct tcttcgttgt agaccaaata acagttttct tcaatcattc
     4501 ctgtttttaa tctttcgata tgcatgtgca ttcctccttg atagccaaaa cttctttcat
     4561 ccattagtat aacgcattca ttgctaggaa acaaaaacag gtacttattt ctctttactt
     4621 atctgaatta tcagactata tgaaaagtat ttggatattc taaagagaag gaaagtaagt
     4681 tttcatagca ttttaatatg tccttgttac attagattca tccaaaaatc caaattctat
     4741 atcctatatc cctgcctaat cagcagggtg tttttttatg tttggaaaga gagctctttt
     4801 tgctaaacta ggttttttca ggtaatctta gagagaaaga ggtggtggaa atgatcaaac
     4861 agtcaaaaat atctgtgcgg cacttagtag aatttatttt gcgacgtgga agtatcgaca
     4921 atcgtaaaaa aagtaaccat actgcattag aaggagcgag aatccaccgc aagctccaaa
     4981 aagatgcggg agaaaactat gaaaaagaag tctttttaaa aacaacagtt caattagatg
     5041 aatattacct gatcgttgaa ggccgtgcag acggtatttt caaaagagac ggtgtttatt
     5101 atatagatta aatcaaaacc tccgaaccac gatttgaaga tttagaacca gaacaagtgg
     5161 aacttttctt tcatcaagca agggtgtatg cctatatcta tagtcaagaa catgatttac
     5221 aagaaataaa tctgcaattg acttacttcc agacggcaga agaagtgatc acgagaaagg
     5281 ttgaacatca gacaagagag cagttagaaa ctttttttaa aaagctgact gaagattata
     5341 aagaatggct tgtttttcaa gaaaactggc gcactgtacg caatgcctct ttgatggctt
     5401 tgaaattccc ccatgatgag tataggaaag gtcagcgtga actagcggta gctgcttata
     5461 aaacgatccg tacgaaacag aaattgtttg tagaagcacc taccggaaca ggaaaaacaa
     5521 tctctacgtt attccccgct ttgaaagctg taggggaaga agagggagaa aagatctttt
     5581 atctaacggc taaaacaata actagacaag tagctgaaga tgctatgaca gcattaaaag
     5641 atacaggagc tgaagtaaaa agtgtcactt tgacggcgaa agacaagatc tgctttttag
     5701 atgagacgac ctgtaatcca gatcagtgcc catatgccaa tggttattac aatcggatta
     5761 atgaaggttt atgggatctt ttgaatcatg agaatcagat cactcgtgaa gtcatcgaaa
     5821 cttatgcaag aaagcataca ctttgtcctt tcgaactatc attagatgtc agtatctggt
     5881 gtgacgtaat cattggggat tacaattatt tatttgatcc aactgtttat ttaagacgtt
     5941 tttttgaaga tgaaaaaaac gaggactatc tgtttttgat tgacgaagca cataatttgg
     6001 tgaatcgttc ccgagagatg tattcggcag aactatctta tgaaaaaacg aaacgaagta
     6061 aggaagctat accaaaggaa ttcaaaaaac ttcatcgccg tttcaacaaa ctattaaaag
     6121 aatttgattc gatccgagaa atagcaaaag aagatcattg ggattatcat catcaaaaag
     6181 caccggctga atctttagta aaggcaggtt atcaattaag cgaaaaaatt aaagaatggc
     6241 tggcagaatt tcctgagcac ccatcacaag aacaattgct tccttattat tttgatttgc
     6301 ttcatttttt gaaggtcagt gaattttatg atgatcacta cgaaacaaca gtcgaaaaaa
     6361 cgtatcgcga tctgatagta aaagagtttt gtattgatcc aagcttgttt cttgagcagt
     6421 cgttggataa aggaaaaagc agtattcttt tttctgctag tttttcacca ctctcttatt
     6481 atcaggaaac actgggggga caaaaaagtc tggcttacaa actgccgagt cctttcccag
     6541 aaggaaacca gcaagttttc atcgcaaatt acctagagac gacttatcga aaaagggaac
     6601 acagtctggc aaaattagta gagacgattc atgcttttgc tgatggaaag gtcgggaatt
     6661 atttcgtttt ctttccttct tatcaatatt tagatcttgc ggtggaagca ttcagacagc
     6721 aatatcccga cagcaatgtg ctaatccaag aaacggacat gaatgaggaa gaacgcgaac
     6781 gatttttaac aaaatttcaa acaaatcccg ctgaaacatt gattggattt tgcgtacttg
     6841 gcgggatttt ttcagaagga atcgatttga gaggggaccg attgatcggc agtatgatcg
     6901 taggtgtagg tttgccgcag atgaatcatg agcaggaact gatcaaatca tattatgatg
     6961 agaaggaaca ccttggattt gcttacgctt atcagcttcc tggaatgaac aaagtcttgc
     7021 aagctggtgg tcgagtgatt cgtggcatgg cggatcaagg aatcgttgta ttggcagatc
     7081 aacgattttc tagtttttct tatcgacgtt tattcccaca acattggcaa acagcaagag
     7141 aagtacgaag tgttggggaa ttggaagaag gaatacagcg tttttggtta tcaaaagaag
     7201 cagtaaatca aaaaaacgag gaatgacaga cgtcagcctc gttttttgat taacgatagt
     7261 tagtgaattg caactcgatt ggtaagtcaa gtttacgtaa taaagcaatc acagcttgta
     7321 gatcatcacg gttcttacct gtcactctaa tctgatcttc ttggatttgt gcttttactt
     7381 tcagtttttc atttttgata gctgtagtga ttttcttcgc gttttcacga tcgattccgt
     7441 tgaccaaatc ggcagtttgt cgagctttgc cacctagagc gtgttctgat tcggaaaaat
     7501 ggatattttt gattggcaca tttcttttga tcagcttacc aaataataca tctttgatct
     7561 gttcgatttt gaaatcatca tcgctcaata caactagatt gctgttctct aatttgattt
     7621 ctactgtaga atccttgaag tcaaagcgat tggacaattc ttttgttgtt tgctgaatcg
     7681 cattttttac ttcttctgta ttgacttctg atacaatgtc gaagctagca tcttttgcca
     7741 taggaaccct cctgatatac atataaatat ttaccataca tctatcttat cataggatta
     7801 gggcagattc ttacctgttt gctaaaattc ttctcatttg agttcgaagt attttatttt
     7861 aatagataaa ctgttattct tacatttgga ataccaaagt aggggagagg tgcttgtgag
     7921 taaaattatg actgcgtatc ataaaaatga aagattaaag aaaatagtgg ttattttgat
     7981 aaccggattg accgctgcgg tcgctttaaa ttattttttg attccagcca atgtcttttc
     8041 tgcagggatg aatgggatcg ctcaaatcat ttcctcgctt ttatatgaat ggtttcatat
     8101 caagatagac acaggaacat ttatttttct tttgaatatc cctgtgtttg tattagggtt
     8161 tctcaaagta ggaaaacgag caacgatcct tagttttgtg aatgttgtat gtgtatcggt
     8221 gatgacgatg ctgcttccag tcggacagat cactgataat attttgatga atgccctagt
     8281 aggcggtgtt ttattaggtc ttggcgtagg attatcttta aaaatgggct ttacaactgg
     8341 tgggatggat atcatctctc ttgtactttc acaaacaact ggaaaaacag tcggaaacta
     8401 catgctgatt ctaaatggta tcattgtagt catcgcaggc ttcttcttta actgggaaag
     8461 tgccttgtat acgattattt ccatttatgc aatgactcaa gtaattgatg ccatccacac
     8521 gagtcatcaa aaagtaacag caatgatcgt taccgtcaag ccagaagagg taacagaaga
     8581 actagcaaaa cggatggtac gtggaatgac attactgccg tctgtcggtg gctattcaaa
     8641 aaaacaaggg aacatgatca tgatggtcat cacccgctac gaaatgtatg atcttgatca
     8701 gatcgttcat gaaatcgacg acgatgcatt tatcaatatt ttaccaactc attcagtatt
     8761 tggccggttt gcaaatgaaa acgaacaaaa aatgtatcga acaactggcg tattgccaga
     8821 aataaaaaat cgttcacaaa aaaaatgagg aatcaggttt attttagaat aaatttaatc
     8881 tattacatct aataagaaca tttttataaa tttaaacgag aatcttactt ctaaattctc
     8941 atttacaagc agctaaaaaa agagtatgat aaataaaaat cgtgttaagg agtgtcgtac
     9001 gatgagcgaa gcaaaccaag aacggtctgg acaagaaata gaactaatca aagaacgtat
     9061 tcttgctgcc ttggaaatgg tgattgatcc tgaattagga atcgacatcg tcaatttagg
     9121 cttgatttat gatatagaat tcaatcctga aaatggagaa acagtcatta agatgactct
     9181 aacaactatg ggctgtccat tagcagatat tttaacagaa tctatccatg acgcattaaa
     9241 agaagtgcca gaagtaacga aagcagaagt caaactagtc tggtatccag catggacgac
     9301 agataagatg agccgttatg cacggattgc gttggggata cgatagaaat cttggctgac
     9361 tagctatgag aagcaaatag gaaaaacaaa aaagcctttt atcctttcta gaagaaaaga
     9421 gataaaaggt ttctttg
//
LOCUS       DAZUMK010000102         9359 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000102 DAZUMK010000000
VERSION     DAZUMK010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.104"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(179..1546)
                     /gene="nhaC"
                     /locus_tag="ACG808_002240"
     CDS             complement(179..1546)
                     /gene="nhaC"
                     /locus_tag="ACG808_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFS5047843.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1655..3076)
                     /gene="tyrP"
                     /locus_tag="ACG808_002241"
     CDS             complement(1655..3076)
                     /gene="tyrP"
                     /locus_tag="ACG808_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFS5047844.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(3319..5196)
                     /gene="tdc"
                     /locus_tag="ACG808_002242"
     CDS             complement(3319..5196)
                     /gene="tdc"
                     /locus_tag="ACG808_002242"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFS5047845.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(5487..6743)
                     /gene="tyrS"
                     /locus_tag="ACG808_002243"
     CDS             complement(5487..6743)
                     /gene="tyrS"
                     /locus_tag="ACG808_002243"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFS5047846.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(6855..7060)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            7323..8624
                     /locus_tag="ACG808_002244"
     CDS             7323..8624
                     /locus_tag="ACG808_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFS5047847.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(8693..9292)
                     /locus_tag="ACG808_002245"
     CDS             complement(8693..9292)
                     /locus_tag="ACG808_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5047848.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT         2829 a         1939 c         1665 g         2926 t
ORIGIN      
        1 tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa cacgtataaa cggcggaagc
       61 agaagcaact ccttcggaaa taagctgaaa ttcacaaaaa tttaaaaagc aattttcttg
      121 agtttcttct tatttcttgg agttaaacgc ttctgtccca gcctcttttt tccttccttc
      181 aattttttac acgtcgacga ttcaataaaa aaccgcctaa tattgtaaag acaggtgcaa
      241 gaatcgagaa aaaggcgaat ggcaagtaat gcaacgcgct aactttcatc gttcccataa
      301 tgaatgtgcc gctgactccc cacggtacga gagaattgac ggtcgcacct gaatccgcta
      361 gtgttcgtgt caaataggtt tttgataagc caacgtcatc aaaagaacgc ttgaatgttt
      421 ctcctggaag aatgatggac agatactgtt ctccaacgat cagattgatt ccgatacagc
      481 tcaaggcagt aaacccgact aaacgagaag gtcggtccat cttttctttt aattcttgaa
      541 cgatggtatc aacgatcaga tatttgatca gtaacccacc taatgccaat gcaagtaaaa
      601 tcaaactagc agaacctaac atactcatga tcccgcctcg agaaagcagt tcatctagct
      661 ttggatcagg tgtatttgcg acataaccat tcatcaagat gtctgctgct tttccgaccg
      721 tcaaactttt ctcatggata aaagaaagaa cgagagcagc tgtcgatccc gctagtaatg
      781 tagggatagc cggaattttt ttgattgccc ccccaaataa aataactagt ggaatcaatg
      841 tccaaggaga gatccaaaag ctcgctttca tgttttggat catttcattg atactagcta
      901 gatcggctga atgactgtta tgtcctaata tcgtataaaa aatcaacgaa aggacgaatg
      961 ctgggaaatc cgtataacgc ataccagcga tatgttcgaa tagattaact cctccaatac
     1021 ccgctgccaa gttggtcgta tctgataaag gcgagcagtt attccctaaa aaggcaccgg
     1081 aaacgactgc gcctgtagtc attgcatcat tgaatccaag taaatgaccg atccccaaaa
     1141 aagcaattcc aatcgtcgaa atcgttgtaa atgaactgcc gacagtcact ccgacaattg
     1201 cacaaacaag aaatgctgtc ggtaaaaaga aacgcacaga aacaagttta aatccataaa
     1261 ccataatcgt agggatggtt ccagataaaa tccatgaagc aactaagaca ccaatcaaaa
     1321 gaaaaatcag gattggaatg atccctggag tgattccttt gatgatcccg tcatggatct
     1381 catcccatgt gaacttttta aatctgccat aaaacaacaa acacatgaaa gctgcaagga
     1441 tcgggatatg tggcgagagc tggaatccga tgatcagtac tcctaggagt gccaacaaca
     1501 cgatcaatac agcgatactt tcttttaaag aaatattttt attcatcgtc gatctcctat
     1561 ttcaaatgaa aaggccctgc catgaaaaaa ctattcactt ggcaggacct cctactaaat
     1621 cagcgacaaa acgtcggctg aaagacttat ttttttattt ttcttctaaa cttttttcta
     1681 cctcaatccc ccaagaatct ttcttgaagt gattgatgat caatggaata acgaacatga
     1741 ccactgtgat agctgctact aataatgcat cactcaatgt cgctgtacct aatgtagcaa
     1801 atacgacaac gatgatagag aagatcagca tgcagctgac tacccaagct aatccgtttc
     1861 cgtttttacc aattcggtaa ggacgttcga attctggatg tttcttacgc attttaagca
     1921 atgaaacagc aatcaaaatg tacacgatac aataaataac agttgtagca tttgtaagtg
     1981 tcaagaatac agcacttacg tcatccatta caccataaag caaagcaaat aatgaaataa
     2041 cgattgattg tgttagaacg acatttctag atacgccgta tttgttttct ttatggaatc
     2101 cgaattttgg tggaaggaat ccttcacgag ctacttggat gattgttttt gatggtccag
     2161 taacccaagc tgataattgt aaaagcacac caataaatac catgaagctg aagatatttc
     2221 cgatcacttc tggtaagcct aggatttgac agtcaatcaa gataggctgc gtgatatttg
     2281 ctaattccat ttttccatct ggtacgacat ttgctacaag catcgcattg atgacattca
     2341 ataaaactaa tccgatcaaa gcgataaata cgccttttgt ataatttttt gttgcgtctt
     2401 tcaaacgagg catgtaaaca gaactcattt cgatcccaac aaaaataaat gtgataccag
     2461 ctaagtattt caatgaatca atatgttcca agtcagggaa agcttttgac catgaaaagt
     2521 cacctagata gccgtttgag actagtccga ctttgaaggc tgccaaaaca cctaaaacga
     2581 acatgatgac tactgggata tataccccta gccatacacc gatgttcccg ccgacttttg
     2641 ccatatcgaa tttcaagttc aagatcgtga tgatccagta gataactaaa atacaaccta
     2701 aaacgaacca gtgattattg ccaagttcta cattaccaaa tgtgtttcct aacaatggtc
     2761 ctaaagtaga agcgaccatt accatacctg ggaacatttg cacccataat agccatgcaa
     2821 cgacaaatcc ccatttgctg cccagtgctg tttttaccca taactgagga ccgccttctt
     2881 gaggaagcat cgtggaaagc tcaccggaaa ccattgatat cggaccggca aaaaacaaaa
     2941 ctgcaaaaat tgaataaaaa atcaatgtcc aaccgactgc ggctaacgta ggaatactcc
     3001 gaaccgttgc acacagtgcc attgtcagac cgataaaggt cccaagaccc attttactta
     3061 cttcagattt actcaccgtt gacactctcc ttgatttttt ggataaaata acggtgtctt
     3121 tgaagtagtt tcagcaataa acgaaacaat tcagcgaata tttgagggga ttctttgagt
     3181 ttattgcttg aagggtttct tcaaagacaa cctcaaaaca aagggatcga agagcttagg
     3241 tctccccttg ctcttcagat ccaaacatgc gtcagaaaca gttagtattc aagctgtttt
     3301 ctaacatgat taccttgctt attttgcttc gcttgccaat aattggtctg cgtagatttt
     3361 ttccaatttt tcttgaagag ctgctttgat tttttcagca tattcttcaa agtgttcttc
     3421 tttgttcatg tatggtgtca ttacgctggc acgcaataca gttacttttc cggcacggtt
     3481 ccattcttca tctgagaatc ctagttggtt cacaaattgt aatgggctgt tgccatagtc
     3541 tgggatagca aagtcagtat gtgaagttaa gaactcatta ccgtaaatgc ttcctttcac
     3601 gtatgaagaa taatcatata cgtcatggtt caatttattc attgcaacta agtcatcgtt
     3661 gcctttttct ttgaatacat agtctaccat attgaaatct ggataagtta atggatgaac
     3721 ttcgatttct ttgtctccta ctttaaatga caagtcattc aagaagttgt agaaacgatg
     3781 tgcaccttcg attgatgcac ccattaattt accatatcct gtgacattca aaggtaagac
     3841 atggtgtgct gcccaaacgc tggctgctgt tgcccctgct tttgaacctt ccaagatata
     3901 agccccaagt aatgctggga tatctgcgcc tttttcaaat acatatgtcg caaagtaaga
     3961 gataacgtct ctcatgcgga tatctttgat gacgatacca ccggcagaat atggaacata
     4021 gcccatttta tgtgggtcaa tcgttacaga ttctgcttct tcgattgctt tatacgcact
     4081 gtgtacttct tctaagatat aatctttgtt ttctgtaaag acattgtatt tgtaatgaac
     4141 atcttttaaa tcttcaaatg gaatgaagtt gttgtcttcg tctaagaaaa tcgcacggcc
     4201 gtatccgcca taagcagcat ctacgtgtaa gtagaagtaa ataccgtctt tttctaatac
     4261 gcgacgcaat gcaacgattt tatcgatacc gtcgattgct ccttcttctg ttgatcctac
     4321 aacaccgact acacctagga ttggtgtttt ttcagctgct agaccacgaa cgattttttc
     4381 aagttcgttg atgtccatgc gatagttgtg gtcaactggc actggaatca cttggtctaa
     4441 accgataccg atgatgtcgg cagcttttag ccatgaatag tgtttagttt gtggaaccaa
     4501 ccattttcct aatttttcta aatgttttcc gctacgtgct gagtgagctt tgatttcgtc
     4561 gattttttct gggacgctgt ctaacagatt catgatttct tctgttgaca agttcattaa
     4621 ttcccaatcg ctttttccag caactagttc aggtgtcact tctttcattg caagcggcaa
     4681 tgatttgatg ttgcgggcat accaaagtcc ttctaagttt gctaaagaac catcagcaac
     4741 gatgtggccc cagccatctt tatagctcat taattttgca aattccattc ctacttcttc
     4801 ttccatttgg cttgttgcag gagaagattc ataggcaacg ttattaccat tccatagcat
     4861 agcaaaattg tacgctagta aagatggcat caatgtttca gagttcatat gaccccagta
     4921 acgtccggca ttatgccaag gaacggaatg ggtacgcatc cgcgctgaaa tttctgatag
     4981 aacatccttt gttttattca cagtgtgttc aaaactagca ctgctttttt cttctggcgt
     5041 aataataggc atgtcttgag gcatgtagtt ttgacgccag cctaaatgtt catccaccaa
     5101 ttcattcaac aaagctttat agatttggcc gttctcagct ttgtccccaa tgaatagggc
     5161 atttaggttt agatctttcg acaatgattc actcatagtg aaaccctcct aaaaaaatat
     5221 ttaaattttg tcgagcccta cattacacct gttcttctat tctgtaaact aagttttggc
     5281 ctgattttta atcatatttt aatatgtatc aattatccga attgttcaca cttttgaaat
     5341 aattaaaaaa aacgagggaa tttacaaatg taaactccct cgcaaagatt gtattaacga
     5401 caaccagcgt tctattttag gatgggtttc aaaattggaa tgttgtaata gtatttccct
     5461 ttttcaaggt aaaaacttac taaattctaa tcaattactt tggccaaaaa gtaatttttc
     5521 ttaccttttc gaataaccac aaacttacca tcgaattcat cggatggatt tatgacaaaa
     5581 tttaaatcgg taacgcggtc accgttaata cttatagctc cgtttgaaac atcttcacgt
     5641 gcttgacgtt tagatggttc aatttttgtg gaaacaagca gttccacgat attttcaggt
     5701 gtgcttgaaa tttcaacgtt cggcatttta ccgaatcctt gcgcgatttc ttccgcattt
     5761 agttctttaa tatttccaga gaacaatgct tgcgttattt tttgcgcttc tttcaagtct
     5821 tcttcactat gcacaaatct tgtaacttct tctgccaaac ggcgttgtgc ttcacgtttc
     5881 tcaggttctg tctctacttt tttcgctaag tcttcgattt cttcttgaga caagaaagta
     5941 aagaatttca agtatttcac tacatcgcga tcatcttgat tcaaccagaa ttggtagaac
     6001 tcaaatggtg aagttttctt cggatcaagc caaacggcac cgcctgcagt ttttccgaat
     6061 ttcgttccgt cagccttcag catcaatggg atcgttaaac cgaacgcttc tgcttctggt
     6121 ccttctaatt ttcggattag gtctaatcca gctgtgatat ttccccattg gtctgctcca
     6181 ccgatttgca attgcacatc gtatgtttta tgcagatgca aaaagtcgat agattgcagg
     6241 atttggtaag taaactctgt aaatgagatt cctacttcca aacggctggc aacgatatct
     6301 tttgctaaca tcgtgttgat gttgaagttt ttaccgtagt ctctcaaaaa ttcaagtaaa
     6361 gagatttttg acaaccagtc gtagttgttc acaaatgtga tatttgcatc tttaccaaac
     6421 aattttctca tttgattgga aagtgcgtcc acgttatgtt gcacttgttc catcgtttgc
     6481 aacacacgtt ctgttttgcg gccgcttggg tcgccgatcg ttcctgttcc gccgccaatc
     6541 agaatatatg gatgatggcc tgccaattgg aatcttttca tcatcataaa tggaatcaaa
     6601 tgtccgatat gcatcgaatc accagttgga tcaacgccgc aataaagtga aatgctcttt
     6661 tcttcaacga gctcgcgaag tctttcttcg tttgtttgct gattaatggc gtcgcgccaa
     6721 gttaattcat caataatatt cattttactg acctccgtgt agttttttta tgaaagcagg
     6781 aacctgctag catgtgtaat taattatttc ttcaggatag gataggaaat aaaaaaagtc
     6841 ccttggcata taagccaagg gacgaaatct cgcggtacca cccaatttgt atgtctagat
     6901 atccaacata cctctcgaac atttaacgct gtcgcgtggc aatcactgcc aaagactcct
     6961 gaaatgtaat tcgctttaaa gcatgtactg gtttgcagcg accaccagct ttctgaaaca
     7021 ttggctttaa caactacttc gtttcgatct tcgtcttgtt attattaata tgtgtctatc
     7081 atagagcgca actttttctt tgtcaacaga aaaaggatat tttttttaaa ttcctcattt
     7141 tttctttttt gaatccgctc taattccgaa cattttattt tcaattattg ttaaaagaaa
     7201 taactttttc tgtatttttt ctgcaattta tcgagaagtc actttacttt gcctcttatt
     7261 ctttcttcgt tacaataagg aatgggacaa gtaaaaaaat gaaaaagcaa gggacgaggt
     7321 atatggaaaa aaagcaaaaa attattttag gagccacttc tgctttaact atcgttattt
     7381 taattctaag tttgtttttt gtcatccgat catctacaaa aaaagaacca atcattcctg
     7441 aaaaaaaaat aacagaaaaa gcaaaagaac cgacagatga taagcaacaa ctagttactc
     7501 aagcaaaaaa tctggctcaa agctacgatt atgaccaagc gattgcgtta ttggaaaaag
     7561 atagtaagaa cggatctact gaaacacaac agcttcttca aacactaaaa aaggagaaag
     7621 aacaactgat cgactggtct gatcctactc agatctctca tgtctttttc cacagcttga
     7681 tcgtggaccc gcaaaaagcc ttccactctc aacaggcaca aggatacaaa gattatatgg
     7741 tgactgttga agaattcaac cgttcgatcg aacaacttta tcaaaatgat tttgttttag
     7801 ttaatttgaa tgatttgatc caaaaagacg aacaaggaaa tctttccttt aacgggcttg
     7861 cgctacctga aggaaaaaaa ccattggttc tttcacaaga tgatgtcagc tattatgaat
     7921 atatggataa ttccggtttt cctagccgat tggtcgtatc aaataacaat gaaatcaaaa
     7981 atgtctataa agagaaagat aaagaagaaa tcggtgactt tgacatggtt cctctgatcg
     8041 atgcctttgt caagaaacat cctgattttt cttatcgagg cgcaaaaggt accttggccc
     8101 tgacaggtta caacggagtt ttaggttatc gaacatcaaa atctcaatat ggagacaatg
     8161 aaaaaacaaa aaaagagatt aaagaagcta aaaaagtggc ggaacagttg aagaaagacg
     8221 gctggagctt tgcttcccat acatggggtc acttgaatat gactcaagct tcactagaag
     8281 atgtaaaaaa agacaatgaa ttgtggcagc aagaagttgc acctatccta ggaaaaacaa
     8341 atattctgat ttatccattt ggtgcagaca tcagcgactg gcagccttat tcagcagaaa
     8401 atcaaaaatt tgcttacttg aagagtcaag gtttcgatat tttctgtaat gtcgatgctt
     8461 ctacaccagc ttggggacaa ttaggtacgg atttctatcg aaatgctcgg atcaatatcg
     8521 atggcattcg ttttcaatct gatttagacg gaaaaaatcc tattcttgat ccattcatca
     8581 atgtccatga tgtctatgac aaagaagcac gcgaatctgc ttgattttac gtcatttatg
     8641 aaccaaaaaa gccgaactga cattttatcg tcagtttcgg cttttttatg ctttatttct
     8701 tcttcggtgc gattcccttg atcaggactt ctgtccattc tgctgtatat ggaagcagtt
     8761 ttgcgtatat ctccggctta gaaattcctt ctaccattgc ttgaaaatag aggatcaaga
     8821 actcatccga atatttcaaa tccacttttc cttctttccg ccctcgatgg aaaagatcaa
     8881 gcatgatact gaaactttct tgcgtatacg catccatcgc gtcagaaaat tctttttttt
     8941 ctttttttgt atagtatgtc atcaaatcga tataaaattg tttgttcact cgattgagat
     9001 aacggatttt gttttcactc atggcaatca gtgtttcttc aaatggaaga ttttccgcaa
     9061 tcaacgcttt cgcttcttgc cctaaatcat tcataaatgc aaaaaatact tcataaatca
     9121 gcccttcctt gctgccaaaa tatttaaaaa tcgtcgcttt acttacctga gcgttctcag
     9181 ctaccttatc cattgtcact ccagcggttc ctatgtctgt gttcatcaag tcaaaagctg
     9241 cttgtaaaat ctctttcttt ttctcttgtg ctctcttttc aaaaccgttc atttgtagag
     9301 cctcccgtaa ttttaaactt ttataatata tatagttcaa atttattctg aggtttatt
//
LOCUS       DAZUMK010000103         9343 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000103 DAZUMK010000000
VERSION     DAZUMK010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.105"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..197
                     /locus_tag="ACG808_002246"
     CDS             <1..197
                     /locus_tag="ACG808_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047849.1"
                     /translation="WFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLIIAIIAVGLV
                     FNAAGVKDILLELFNRIIGA"
     gene            314..811
                     /locus_tag="ACG808_002247"
     CDS             314..811
                     /locus_tag="ACG808_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFS5047850.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            898..1290
                     /locus_tag="ACG808_002248"
     CDS             898..1290
                     /locus_tag="ACG808_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS5047851.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            1274..3721
                     /locus_tag="ACG808_002249"
     CDS             1274..3721
                     /locus_tag="ACG808_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5047852.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSDGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            3724..5907
                     /locus_tag="ACG808_002250"
     CDS             3724..5907
                     /locus_tag="ACG808_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFS5047853.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            5904..6905
                     /locus_tag="ACG808_002251"
     CDS             5904..6905
                     /locus_tag="ACG808_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFS5047854.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            6920..7834
                     /locus_tag="ACG808_002252"
     CDS             6920..7834
                     /locus_tag="ACG808_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS5047855.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            8148..8195
                     /locus_tag="ACG808_002253"
                     /pseudo
     CDS             8148..8195
                     /locus_tag="ACG808_002253"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8211..>9343
                     /locus_tag="ACG808_002254"
     CDS             8211..>9343
                     /locus_tag="ACG808_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFS5047856.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQY"
BASE COUNT         2966 a         1741 c         1981 g         2655 t
ORIGIN      
        1 actggtttct atcaaacgct caatcactgg tgcttttagc aatcgttgtg attggcttgt
       61 atcttggctt caagcgtgag tttagcaaac tgattggctt tttaattatt gcgattattg
      121 cggttggctt agtcttcaac gctgctggag taaaagacat tttactagag ctattcaatc
      181 gcattattgg tgcttaaata aaaccgttct tttgtggaat ataagtggtt ttcttatgtt
      241 ccgcaaagga atggtacacc aaacgaagtg cggtagggat ttttgaatct ctacaaagaa
      301 aggacgtgaa tatatggacg atatgcaagt ctatattgcg aatttaggca aatacaatga
      361 gggcgaattg gtcggtgcgt ggtttacctt tcccattgac tttgaggaag tcaaagagaa
      421 aatcggcttg aatgatgaat atgaggaata cgccattcat gattacgagt taccctttac
      481 ggttgacgaa tacacttcta ttagcgaact caatcgccta tgggaaatgg tatcggagtt
      541 gccagaagaa ctacaatcgg agctatctgc tctgctcact catttttcaa gtattgaaga
      601 actaagcgaa catcaagaag atattatcat tcattccgat tgtgatgata tgtctgacgt
      661 ggcacgctac tacattgaag aaacgggtgc tttaggcgaa gtgccagcca gtcttcaaaa
      721 ctatattgat tatgaatcct atggtcggga tttagacctt tcgggaacgt ttatctcaac
      781 caatcacggg atttttgaaa tcgtctatta aatctgtcgg tacattctta ctggcagatt
      841 ttcttgttta cggggtagtc aaaagactat ccccattttt atgaaaggat tgattacatg
      901 aagaaaatac gaagctatac cagtatctgg tctgttgaga aagtgttgta ctccatcaat
      961 gactttagac ttccgtttcc cataaccttt acgcaaatga catggtttgt cgtgtcactc
     1021 tttgcagtta tgatacttgg caacttgccc cctctttcta tgatagaggg agcatttctc
     1081 aaatactttg ggattcctgt ggctttcaca tggtttatgt ctacaaaaac ctttgatggt
     1141 aaaaagcctt atggattttt gaagtctgtc attgcttatg cactgcgacg aaagctgacc
     1201 tatgcaggaa aaaaagtaac gcttggcaga aaccagccac aagaagccat tacagcagtt
     1261 aggagtgaat tttatggcat atccaattaa atatattgaa aacaatctgg tctggaataa
     1321 agatggggaa tgttacgctt actatgagct tgttccctac aattactcat ttctaagtcc
     1381 ggaacagaaa atacaagtac atgattcttt cagacagctt atcgcacaaa atcgtgatgg
     1441 caagattcat gctttacaaa tcagtacaga atccagcata ctttctgcac aagaacgttc
     1501 caaaaatgaa gtcactggaa agctcaaaac ggttgcctat gacaaaatcg accaacagac
     1561 agacgcttta atatccatga ttggcgaaaa tcaagtggac taccgtttct ttatcggttt
     1621 taagttgctt ctcaacgatc aggagttttc tatgaaaagt cttaccgttg aagcaaaaaa
     1681 tgcttttact gattttgtct atgatgtgaa ccataagctg atgggcgatt ttgttagtat
     1741 gagtaatgat gaaatcctgc gttttcagaa gatggaaaag cttttagaaa ataaaatctc
     1801 ccgtcgtttc aaaatccgaa gattagataa ggatgacttc ggctatctga ttgaacacct
     1861 ttacggacag acaggcactg cctatgaaga gtatgagtat cctctatcaa agaaaaagct
     1921 ggaacatgaa acactgatta aaacctatga cctcattaag cctactcgct gtctggtgga
     1981 agaaaaacag cgatatttga aaatccagca ggaagacgaa accgcctatg tagcttactt
     2041 taccatcaac agtattgtcg gcgaactgga cttcccgtcc tctgaaatct tctactacca
     2101 gcaacagcaa tttacctttc cgattgatac gtcaatgaat gtggaaattg tagcgaatcg
     2161 taaagcccta tctactgtcc gcaataaaaa gaaagaactg aaagacttgg ataaccacgc
     2221 ttggcaaagt gataatgaaa ccagctctaa tgtggcggaa gctctggaaa gtgtgaacga
     2281 gctggaaacc aatttagacc aaagcaagga atctatgtat aagctgtcct atgttgtaag
     2341 ggtatcagca aatgaccttg acgaacttaa acgtcgttgt aatgaagtga aagatttcta
     2401 tgatgatttg agcgtgaaac tggtacgacc ttttggggat atgctgggct tacatgaaga
     2461 atttttacct gccagcaaaa gatatatgaa tgactatatt caatacgtga cctctgattt
     2521 cctcgctggt ttaggttttg gtgctactca aatgctgggt gaaaatgagg ggatttatgt
     2581 tggctacagc ttagatactg gacgcaatgt ctatctgaaa cctgctcttg ccagtcaagg
     2641 ggttaagggt tcagtaacca atgcgttagc gtctgccttt gtcggttcgc tgggtggtgg
     2701 taaatccttt gcgaataacc ttatcgtcta ttatgcagtg ctttatgggg cacaagcagt
     2761 gattgtagac ccaaaagcag aacgtggcag atggaaagaa accttgccag agatttccca
     2821 tgaaatcaat atcgtcaatc tgacttctga tgagaaaaac aaaggcttac ttgaccctta
     2881 tgtaattatg aaaaatacca aagattctga atcactggct attgatattt tgacattcct
     2941 tacggggatt tcctctcgtg atggggaacg cttcccaatc cttagaaaag ccattcgtgc
     3001 agtaaccaat agtgaagtgc gagggttgat gaaagtgatt gaagaattac gggttgagaa
     3061 tacgccacta agtaccagca tagccgacca tatcgaaagt tttacagact atgactttgc
     3121 ccatctgctt tttagtgatg gttatgtaga gcagtctatc agccttgaaa aacaactgaa
     3181 cattatacag gttgccgact tggtacttcc tgacaaggaa acttcctttg aggaatatac
     3241 cacaatggag ttactatcgg tagcaatgct gattgtcatt agtacctttg cgttggactt
     3301 tatccataca gaccgaagta ttttcaagat tgtagactta gacgaagcat ggagcttctt
     3361 acaggtggca caagggaaaa cactatctat gaagctggtt cgtgctggtc gtgctatgaa
     3421 cgctggggta tatttcgtga cccaaaatac agatgacctc ttagatgaaa aactgaaaaa
     3481 taacctcggc ttaaaatttg cctttcgttc cactgacctt aacgagatta aaaaaaccct
     3541 tgcctttttt ggtgtagacc cagaggacga aaacaatcag aagcgattgc gtgatttgga
     3601 aaacgggcaa tgccttatca gtgatttata tggtcgtgtc ggtgtgatac agttccaccc
     3661 tgtatttgaa gaactgctcc atgcctttga taccagacca cctgtgcgaa aagaggtgta
     3721 aatgtgaaac catcaatatt aaacagaata aaatcaaact ggacgctgaa acgtctaggt
     3781 aaagtggcaa tgacagtggc tttcacactt gtgattgcca tttttctttt agccatgctg
     3841 ggaacggtgg ttcaagctgc gggcttggta gatgatacgg tcaatgtggc aaatgaatac
     3901 agccgatacc cacttgaaaa ctatcaactg gatttttatg tggataatag ctggggctgg
     3961 ctaccgtgga actggtcgga cggaattgga aaacaagtca tgtatggact atatgccatt
     4021 accaatttta tttggacaat cagtctttat gtttccaatg cgacgggtta cttagtacag
     4081 gaagcctatt ccttagactt catttccgct acagcagatt ccattggcaa gaatatgcag
     4141 acccttgcag gggttacgcc tagtggacta tcaacagagg gtttctatgt tggattcctt
     4201 ttactcttga ttttggttct tggggtttat gttgcctata caggactgat aaagagagaa
     4261 accacaaagg caattcatgc cctcatgaat tttgtgctgg tttttatcct atcggcttcc
     4321 tttattgcct acgctcccga ctacattaaa aaaatcaatg acttttcatc agacatcagc
     4381 aacgctagtt tgtcactggg tacaaaaatt gtcatgcccc attcagatag tcaaggcaag
     4441 gacagtgtgg acttgatacg tgacagccta ttttccatac aggttcagca accgtggcta
     4501 ctgcttcaat ataacagttc agacattgaa agtattggta ttgaccgtgt ggaaagcctg
     4561 ctctcaacca gtccagattc caacaatggg gaagacagag aaaaaattgt tgcggaagaa
     4621 attgaagata gaagcaatac caatatgacg attacaaaga cgattaaccg tttaggtaca
     4681 gtcttcttcc tatttgtctt caatattggg atttccatat ttgtattcct attaacagga
     4741 atcatgattt tctcgcaggt actttttatc atctatgcta tgtttctgcc tgtgagcttt
     4801 attttaagca tgattccatc atttgatggt atgtcaaaac gagccataac aaagctcttt
     4861 aataccattt tgacacgagc tggaatcaca ttgattatta cgacagcatt tagtatttca
     4921 accatgctct ataccttatc ggctggttat ccgttctttt tgattgcttt tctacagatt
     4981 gtgacctttg caggaatcta ctttaagctg ggcgatttaa tgagtatgtt ttctctacag
     5041 agtaacgatt ctcaaagtgt gggaagtcgt atgatgagaa aacctcgtat gcttatgcac
     5101 gctcacatgc accgtctaca gcggaaactt ggacgctccg tgactgcttt aggggctggg
     5161 tctgccattg ccagtgctac gggtaaaaaa ggacagtcgg gttcgggaag ttctgcgagt
     5221 acacaagcag atcactctcg accaaacggg caggaaaaat caacactagg aaagcgtatc
     5281 ggtcaaacca tcggtacagt agctgatacc aaagacagaa tggtagatac tgctggtaat
     5341 ttgaaagaac aggttaagga tttgccgacc aatgcaagat atgcagtata tcaaggaaaa
     5401 tctaaagtga cggataatgt ccatgattta accagaagta tttctcaaac caaagcggat
     5461 aaagccagtg gacgcaagaa acagcaggaa caaagacgga aaaccattgc ggagcgtcgc
     5521 tctgaaatgg aacaggtcaa acagaaaaaa cagccagctt cttccgttca tgaaagacca
     5581 gccactaagc aagaacaatc tcatgatgga cagacttcaa ggcaaacaac tgtacaggct
     5641 tcctataggg aatctcaaca agccaaacaa gagcgtccaa cagttaagtc cgattcttca
     5701 agtttaaaaa cggaacgtca aagtaataca gttcaagaaa gaacggttca aaagccagta
     5761 acttcaactg caccaacaga tagagcttca caacgttcaa tcacaaaaga acgtcagtct
     5821 actgttcaaa gagtaccact acaaaataca aaaagtagac caccaatcaa aaccgccacc
     5881 attaagaaag gcagtaagaa accatgaagc tgaaaacttt agtgattggt ggttcgggat
     5941 tattcttgat ggtcttctca ctgcttctgt ttgtcgccat tttattttca gatgaacaag
     6001 atggcggttt ttccaacatt cactatggcg gtgtggatgt ttccgcagaa gtgctggctc
     6061 ataagcctat ggtagaaaaa gttgccaaag aatatggaat tgaagaatac gtcaatatac
     6121 ttcttgcgat tatacaggtg gaatcgggtg gtacagcaga agatgttatg cagtcctcgg
     6181 aatccctcgg tcttccacct aattcattga gtacagaaga atccattaag caaggtgtga
     6241 agtatttcag tgagttatta gccagtagcg aaaggcttgg tgtagattta gaatcggtta
     6301 tccagtccta caattatggt ggtggtttct taggatatgt ggctaatcgt gggaataaat
     6361 atacctttga actggctcaa agtttctcaa aagagtattc gggtgacgaa aaagtgtctt
     6421 accccaatcc catagccata cctatcaatg ggggctggcg atacaattac gggaatatgt
     6481 tttatgtgca actggtaacg cagtatcttg tcacaacaga gtttgatgat gatacagtac
     6541 aagccatcat ggacgaagct ctgaaatttg aaggctggaa gtatgtctat ggcggagctt
     6601 ccccgactac ctcttttgat tgtagcggac tgacacaatg gacgtatgga aaagctggaa
     6661 tcaacttacc acgaacagca caacagcaat atgatgtaac ccggcatatc ccattattgg
     6721 aagcaaaagc cggcgatttg gttttctttc attctaccta taacgctggc tcttatatta
     6781 ctcatatcgg gatttattta ggcgataacc gtatgtttca tgcaggcgac cctatcggtt
     6841 atgccgactt aacaagctct tactggcaac agcatttagt gggagcagga cgaatcaaac
     6901 aataagaaag gacgattcaa tgatgaaatt cggaaaaaat cagaatacag aaaaacagac
     6961 accaaaggaa aagaaacctc gtgtctataa ggtcaatcct cgtaaaaagg ttgtgattgt
     7021 cctatgggta cttttaggac ttagtttcag ctttgcgata ttcaagcact ttacagccat
     7081 tgatacccat accattcatg aaaccaccat catagaaaag gaatatgtcg atactcatca
     7141 tgtggaaaat tttgtagaga actttgcgaa agtctactat tcatgggaac taaacgacaa
     7201 gtccattgat aatcgcatag aaaacctgaa agcatatctg acagaggaac ttcaagctct
     7261 gaatgtcgat acagttcgta aagatattcc tgtatcgtct tctgtaaaag gatttcagat
     7321 atggacggta gaagcagatg gcgacaatca atttgatgta acctacagtg tagaccaact
     7381 cattacagag ggggaaaata caaagaccgt ccactctgct tatatagtga gtgtctatgt
     7441 agacagtact ggaaatatgg tcctcattaa gaatccgacc attactagca taccaaagaa
     7501 atcagactat aaaccaaaag ctattgaaag tgagggtacg gttgattcca ttacaaccaa
     7561 tgaaatcaat gagtttttaa cgacgttctt caagctctat cctacagcga cagccagtga
     7621 actttcctac tatgtgaatg acgggatatt aaaaccaatc ggaaaagagt acatctttca
     7681 agaactggta aatcctattt acaatcgtaa ggataatcaa gtcacggtat cgctgacagt
     7741 ggagtatatc gaccagcaga ccaaagcaac gcaggtatct caatttgatt tggtacttga
     7801 aaagaacggg agtaattgga agattataga ataacaaata ttggtacatt attacagcta
     7861 ttttgtaatc acgtactctc tttgataaaa aattggagat tcctttacaa atatgctctt
     7921 acgtgctatt atttaagtat ctatttaaaa ggagttaata aatatgcggc aaggtattct
     7981 taaataaact gtcaatttga tagtgggaac aaataattgg atgtcctttt ttaggagggc
     8041 ttagtttttt gcacccagtt taagaatacc tttatcatgt gattctaaag tatccggaga
     8101 atatctgtat gctttgtatg cctatggtta tgcataaaaa tcccagtgat aagagtattt
     8161 atcactggga tttttatgcc cttttgggct tttgaatgga ggaaaatcac atgaaaatta
     8221 ttaatattgg agttttagct catgttgatg cgggaaaaac taccttaaca gaaagcttat
     8281 tatataacag tggagcgatt acagaattag gaagcgtgga caaaggtaca acgaggacgg
     8341 ataatacgct tttagaacgt cagagaggaa ttacaattca gacaggaata acctcttttc
     8401 agtgggaaaa tacgaaggtg aacatcatag acacgccagg acatatggat ttcttagcag
     8461 aagtatatcg ttcattatca gttttagatg gggcaattct actgatttct gcaaaagatg
     8521 gcgtacaagc acaaactcgt atattatttc atgcacttag gaaaatgggg attcccacaa
     8581 tcttttttat caataagatt gaccaaaatg gaattgattt atcaacggtt tatcaggata
     8641 ttaaagagaa actttctgcc gaaattgtaa tcaaacagaa ggtagaactg tatcctaata
     8701 tgtgtgtgac gaactttacc gaatctgaac aatgggatac ggtaatagag ggaaacgatg
     8761 accttttaga gaaatatatg tccggtaaat cattagaagc attggaactc gaacaagagg
     8821 aaagcataag atttcagaat tgctccttgt accctgttta tcatggaagc gcaaaaagca
     8881 acatagggat tgagcagctt atagaagtga taacgaataa attttattca tcaacataca
     8941 gaaagaagtc tgaactttgc ggaaatgtct tcaaaattga atattcggaa gaaagacaac
     9001 gtcttgcata tgtacgcctt tatggcggaa tcctgcattt gcgggattcg gttagaatat
     9061 cggaaaagga aaaaataaaa attacagaaa tgtatacttc aataaatggt gaattatgta
     9121 aaattgataa ggcttattcc ggggaaattg ttattttgca aaatgagttt ttgaagctaa
     9181 atagtgttct tggagataca aagctattgc cacagagaga gagaattgaa aatccgctcc
     9241 ctctgctgca aacaactgtt gaaccgagca aacctcaaca aagggaaatg ttacttgatg
     9301 cacttttaga aatctccgac agtgacccgc ttctacaata tta
//
LOCUS       DAZUMK010000104         9085 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000104 DAZUMK010000000
VERSION     DAZUMK010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.106"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..281)
                     /locus_tag="ACG808_002255"
     CDS             complement(<1..281)
                     /locus_tag="ACG808_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS5047857.1"
                     /translation="MSITKNIKCTDEKTSHSIKQILNIKDSHITFSKDAISKERVHGR
                     MANVFTGLLTGEAHRCECCGFDSVIRHAYHDSWIQLLPYQEVPTYLHLY"
     gene            complement(396..662)
                     /locus_tag="ACG808_002256"
     CDS             complement(396..662)
                     /locus_tag="ACG808_002256"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047858.1"
                     /translation="MVYFRKRLDETTLIEINEKILVFDKKKDDKDNDSYDDFNSGTLI
                     LDATCAPQNIKYPTDTELMNDTRTHAEKIIDVVCEGKKLKKPVL"
     gene            complement(716..805)
                     /locus_tag="ACG808_002257"
     CDS             complement(716..805)
                     /locus_tag="ACG808_002257"
                     /inference="COORDINATES: protein motif:HMM:NF017416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047859.1"
                     /translation="MTFGALLIQKEYSYSDEETVLQIQENPYL"
     gene            complement(802..987)
                     /locus_tag="ACG808_002258"
     CDS             complement(802..987)
                     /locus_tag="ACG808_002258"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047860.1"
                     /translation="MYKLQTTVQLAFEDFNQPIGLKMNPSNRWIQKAELIPWAELEKD
                     YAKNFRKKKETSQNPFA"
     gene            1156..3267
                     /locus_tag="ACG808_002259"
     CDS             1156..3267
                     /locus_tag="ACG808_002259"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5047861.1"
                     /translation="MNTKDLKLLETIVRSHVTNSQQLKSLVHFSTRQITYSIKKINDE
                     LSLHGLSLIKRSNSGEWLYEANDLQKLLIYMNQAMVFKEGAIPEYINDDLAYSEIRRY
                     SELLFILANPKKVTTQEIMAFIGISKNTARNDFSKIKKELPPDYTLIFDPQNGYMIES
                     SMAKLHTLLLEVFNYLFERHATVNRFESLFSEEREFVGFFIHTYEQTFKLKFSDNSLT
                     KLYYLIGLSLSSFKRRTKGQIDFSYDLQELDEFQMIRSCIDQHYQHAEIAIDYEWLTL
                     LFLSFNTISNEFEILDSRIYQVIMEMITLFQSKSAIRISNKEELTNRLMNHLRPAIYR
                     IRYELPFEIDDLAEIVKENHEVSAVLNFVKESIVPLEAFLRKTIPETELYLISVYFCS
                     ELLNSSIIETPVRKKAIVVCSNGLIMANVMAKTLKEIFPDIQFVASSSVREMDKFVED
                     VDLIFSNQPLKSEIPLYIIQPIMNEEEKNRLRNRVLIDFSTNNISKETRNLTEIIKRY
                     SAVQNEESLQTDIERFLINYANSLIYLDRQSKQAQKISFVELLVSEGIILVSTKQLTW
                     QNAMELSCQPLLKEQVINQNYQTILMSQMADEEAYYFLNNRIAIPHSSPENGVIKGGC
                     SLLISVDPIIFPGNRPIHFISPFAIGKKENYLLAINQLLQLSKDEEYQQEILLCTTED
                     EVRKKIKNFLRRKQYVNNEFS"
     gene            3245..3895
                     /locus_tag="ACG808_002260"
     CDS             3245..3895
                     /locus_tag="ACG808_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFS5047862.1"
                     /translation="MSITNFPELTIILRGYSFEEAMFVMEELAAMNTSVGIEVTTNND
                     DFARIITEGNKKFGQQLLIGAGTVLTFSQADQAIAGGAKFLLGPKKFSTPIFSLAKER
                     GVVTVPSAFTPSEIQVMFDEGADIVKVFPAVTVGPTYFKQLAGPFGIRPLMAVGGVTI
                     DNIAAFKKAGVVYFGVGSSMFQKADIQNRNGQAIRKSIQSYLDKLAEAGSEEDESH"
     gene            3882..4334
                     /locus_tag="ACG808_002261"
     CDS             3882..4334
                     /locus_tag="ACG808_002261"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5047863.1"
                     /translation="MSLIKLEEKNILLNQSFSNQEEYFQKITKKLMTENMVKEGYLES
                     ITKRETTYPTGLYIGERSIAIPHTDYQLSNVTAILVSVLKDSLHFQRMDDPEEMVSVN
                     VIIHLLFDSPDKQPQLLKELMSKVTNQDFLQAIMEAKSKTEVLTAFNQ"
     gene            4351..4656
                     /locus_tag="ACG808_002262"
     CDS             4351..4656
                     /locus_tag="ACG808_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIB"
                     /protein_id="HFS5047864.1"
                     /translation="MSKKTIIVACASSMITSTMAANKIRKIAKTNGLPEPRILQLKFS
                     EVAGALESNQVDFIVPTGKLQENVTKGIKVIPGTPFVTGVNEEKSESLVLEALKELD"
     gene            4668..5945
                     /locus_tag="ACG808_002263"
     CDS             4668..5945
                     /locus_tag="ACG808_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFS5047865.1"
                     /translation="MNIVIDALNWISSLGPMIMMPIIILILGLFFRIKLNTLLKSALT
                     VGIGFAGVNIIINWFISQVGPSVQLMVKNWGIQTSIMDVGWPARAAATWSFPLAAVMV
                     FIILGINVLMLIFKKTNTVMVDFWSYNHYIFVAALVYYATDKNIILAVIFGAIDAIIS
                     FKLADWTTPLVEDYFELEGVNFPTANSVGWAPLAWLLNKLWDRIPGLNKVDARPEKIQ
                     ERFGFVGEPMFMGFVIGLVIGLLAKEPVDKVLIVGMSTSATMVLTPKMMQILMEGLLP
                     FANSVKKFLNVKFPGNNFTMGVDAALTVANSSAIATGIIMVPITLVLAAILPGNKLLP
                     ISDIAYQAMWLSAWPVAFSKGNIIRGILSTTIITACVLLIATSLAGIHTQLALAGGFQ
                     LPAGMTQISTEDAGTHIIGFILMKIAEFISNIF"
     gene            5964..7112
                     /gene="dgoD"
                     /locus_tag="ACG808_002264"
     CDS             5964..7112
                     /gene="dgoD"
                     /locus_tag="ACG808_002264"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HFS5047866.1"
                     /translation="MKISKITVYKVKPRWIFVKISTDEGIEGWGEMISGTKTETVVAG
                     AYEIGNRFLVGQNPFAIERLFQEMHRSFFRGGPINGTIVSGLEMALWDIKGKALNVPV
                     YELLGGAARDKIKVYSWIGGDRPSEVVEQAQDRVDRGFTAVKMNATEELHYIDSYEKV
                     AEVVERVASIRERFGDTLSIGVDFHGRVHKPMAKVLAKALEPYHPMFLEEVVLPENEE
                     HFSEVANAVAVPLATGERLYTRWQFKSILQQGAIDIIQPDVALCGGILETRKIAAMAE
                     AYDIAVAPHAPYGPIALAATLQVDSCTPNVFIQEQSLGIHYNKGFDLLDFVKNKEIFQ
                     YKDGFVDLPTKPGLGLEMDEDRVKEIAQEGLVWTNPKWKNYDGTIAEW"
     gene            7124..8026
                     /locus_tag="ACG808_002265"
     CDS             7124..8026
                     /locus_tag="ACG808_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase"
                     /protein_id="HFS5047867.1"
                     /translation="MINTVTGPIEAESLGTTFIHEHLYVIPNELRKYEDYTLDDIYHS
                     IAEVMAFKQAGGKTIVDLTPINYGRNPLALKMISEATGVNVAFVTGFHKEEFQPKWLT
                     TLNDDEIFELLYSEITHGVTSQNLLPGAMKVGTSKNEVTLAEQRILAIAGQVQKKTGI
                     PIITHCDGGTMGLEQINCLTKNGAKASNICLSHVDLTKDFHYLAQICETGASICMDHI
                     GRELANHDADRVQLIKRLVDAGYENQICLSGDMGRKKYLPTYGGTPGLTYILTSLKEE
                     LLKVMSEPTFNKMIEMNPINILSK"
     gene            complement(8695..>9085)
                     /locus_tag="ACG808_002266"
     CDS             complement(8695..>9085)
                     /locus_tag="ACG808_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5047868.1"
                     /translation="FKQFPLFQGKYMTESEVLDYLLSIDNQLRQSYEVYQELLAAFDA
                     KDFTEFFNLIESLPHSLNDGFKKAICYLKKHTEAITNSLKYPYSNGKLEGKNNLIKVI
                     KRIAFGFRTFRHLRMRVLIQQNICEII"
BASE COUNT         2948 a         1397 c         1855 g         2885 t
ORIGIN      
        1 ttgtacaggt gtagataagt tggtacttct tgatacggaa gcaactgaat ccaactatca
       61 tgataagcat gacggatgac agaatcaaac ccacaacatt cgcaacgatg cgcttctcca
      121 gttaataagc cggtaaatac attggccata cggccatgaa cgcgctcttt actaatggca
      181 tcttttgaaa aggtaatatg ggaatctttg atgttcaaaa tttgtttgat ggaatgactt
      241 gtcttttcat ccgtacattt gatattctta gttatggaca tttggttatc ctcctatgat
      301 tgtttttgcc gatttaatta taggacaacc agatgtcttt ttgttataaa aaaatatttg
      361 gcgtcactac tcagtttgag taacaaaacc aaatattata gaaccggctt tttcagtttt
      421 ttcccttcac aaacaacatc gatgattttc tcggcatggg tacgggtatc attcattaat
      481 tctgtatcgg ttggatattt gatgttttgt ggagcacaag tggcatccaa aatcaatgtt
      541 ccggaattga aatcgtcata agaatcgttg tccttatcgt cttttttctt gtcgaagacg
      601 agtatttttt cattgatttc tatcaacgtt gtttcgtcca agcgtttccg gaaatagacc
      661 attgtagagc atcgaaaggc ttggcatctt gatagcccgg caaaccgatg aagaactata
      721 aatacgggtt ttcttgaatt tgtaagaccg tttcttcgtc gctatagcta tattcctttt
      781 gaatcagtag agctccgaag gtcatgcgaa agggttttgc gacgtttcct tttttttacg
      841 aaaattttta gcgtagtctt tttcaagttc agcccatgga atgagctctg ctttttgaat
      901 ccaacggtta gacggattca ttttgagacc tatcggttga ttgaaatctt caaaagccaa
      961 ttgaacggtt gtttgtaatt tatacatgaa aagcctccaa aattgagtgg tactccacac
     1021 cattctgatg gtgcataaga taaaaaatta aagaagtgcc tgtcatttgt ctgatttaag
     1081 ccaaggtttg acaaattttg gatattccaa ttaatactgt aagcggttat atttaactca
     1141 gggaggtaga tgaatgtgaa tacaaaagat ttaaaactgt tagaaaccat tgtacgatca
     1201 catgtgacaa actctcaaca attgaaatca ttagtacatt tttcgactag gcagataact
     1261 tactctataa aaaaaattaa tgatgaatta tcccttcatg gattgagtct gataaagcga
     1321 agtaactcag gggagtggtt atatgaagcc aatgatcttc aaaaactttt gatatacatg
     1381 aatcaagcaa tggtttttaa agaaggtgcg attcctgaat atataaacga tgatttggct
     1441 tatagtgaga taagaagata ttcggaactg ttatttattc tagccaaccc caaaaaagtg
     1501 acaacccaag aaataatggc gtttattggt atcagtaaaa atacggctag aaacgacttt
     1561 tctaaaataa aaaaagagtt gccaccagac tacactttga tatttgatcc ccaaaatggc
     1621 tatatgattg aatcaagcat ggcaaagctt catacattgc tcttagaagt tttcaattat
     1681 ttatttgaaa ggcatgcaac agttaaccgt tttgagtcac tcttttctga agaaagggaa
     1741 tttgttggtt tttttattca cacctacgaa caaactttta aattgaaatt ttctgataat
     1801 tcactaacca aactttatta tttgataggt ttatcacttt ctagttttaa aagaaggacg
     1861 aaaggacaga ttgattttag ttatgattta caggaattag atgagtttca aatgattcga
     1921 agctgcattg atcaacatta tcaacatgct gagatagcta tagattatga atggttgacg
     1981 cttctgtttt tatcattcaa tacaatttca aatgagtttg aaattttaga ttcaagaatc
     2041 tatcaagtaa ttatggagat gattacctta tttcaaagta aatcagctat tcggattagt
     2101 aataaagaag agttgaccaa tcgattgatg aatcatttac gacctgcaat ctatcgaatt
     2161 cgctacgaat taccatttga aatagatgat ttagctgaaa ttgttaagga aaaccatgaa
     2221 gttagtgcgg tacttaattt tgttaaagaa tccattgtac ctttggaagc atttttgcgt
     2281 aagacgattc ctgaaactga attatatttg atttctgttt atttttgttc ggagctatta
     2341 aattcttcta ttatcgaaac acctgttaga aaaaaggcaa tcgtcgtatg tagcaatgga
     2401 ttgattatgg ccaatgtaat ggcgaaaaca ttgaaagaaa tttttccaga tattcagttt
     2461 gttgctagtt cttcggtcag agagatggat aagtttgtcg aagatgttga tttgattttt
     2521 agcaatcaac cattgaaaag tgaaattcca ttatatatta tacaaccgat tatgaacgaa
     2581 gaagaaaaaa accgcttgcg gaaccgtgta ctcattgatt tttctacgaa taatatcagc
     2641 aaagagacta ggaaccttac tgaaattatt aagcgttact ctgctgttca aaatgaagaa
     2701 agtctacaaa ctgatattga gagattttta attaattatg caaattcact aatatatcta
     2761 gatcgtcaaa gtaaacaggc acaaaagata tcttttgtgg aattgcttgt ttctgaaggg
     2821 attattcttg tatctacgaa acaacttact tggcagaacg cgatggaatt gtcttgtcag
     2881 cctttattaa aggagcaggt aattaatcag aactatcaga ctattttaat gagtcaaatg
     2941 gcggatgaag aggcttatta ttttcttaat aatcgtattg ctattccaca ttcatcgcca
     3001 gaaaatgggg taattaaggg cggttgctcg ttacttatat cagttgatcc aattattttt
     3061 cctggaaata ggcctatcca ttttatttcc ccttttgcaa ttggaaagaa ggaaaattat
     3121 ttacttgcga ttaaccaatt actacaactt tctaaggacg aggaatatca gcaagaaata
     3181 cttctttgca cgacggaaga tgaagtgaga aaaaaaataa aaaatttttt aaggaggaag
     3241 caatatgtca ataacgaatt ttcctgaatt aaccatcatt ttaagaggat atagttttga
     3301 agaggcgatg tttgtcatgg aagaattggc tgcgatgaac acttcggtag gaattgaagt
     3361 aacaaccaat aatgatgact ttgcacgtat catcacagaa ggcaacaaaa aatttgggca
     3421 acaattattg attggtgcag ggacagtttt aacattttca caagcagatc aagcaattgc
     3481 tggtggtgcg aaatttttat taggaccaaa aaaattttca acaccaattt tttcgttggc
     3541 taaagaacgt ggggtggtaa ctgtgccatc agcttttacg ccatcggaaa ttcaagttat
     3601 gtttgatgaa ggtgccgata ttgtaaaggt ttttccagca gtcactgtag ggccaaccta
     3661 ctttaaacaa ctggccggtc cttttggtat acggccttta atggcagttg gaggagtgac
     3721 gattgataat attgcggctt ttaaaaaagc aggagttgtg tactttgggg taggatcaag
     3781 catgtttcaa aaagctgata tacaaaaccg caatgggcaa gctattcgaa aatcaattca
     3841 atcatattta gataaactag ctgaagcagg aagtgaagaa gatgagtctc attaaattag
     3901 aagaaaaaaa tattttgtta aatcaaagtt tttcaaacca agaagaatac tttcaaaaga
     3961 ttacaaagaa actgatgacg gaaaacatgg taaaggaggg atatctcgag tcaatcacta
     4021 agagagaaac aacttatcca acggggctat atattgggga acgctcaatc gcaattccac
     4081 atacagatta tcagttatca aatgttaccg ctattttagt atccgttctt aaagatagtt
     4141 tgcattttca gcgaatggat gatcccgaag aaatggtttc agtgaatgtt attattcacc
     4201 ttttattcga tagtcccgat aagcaaccgc aattattgaa ggagttgatg agtaaagtta
     4261 ccaatcagga tttcttacaa gcaattatgg aagcaaagag taaaacagaa gttttaactg
     4321 catttaatca ataaattgga gggaaataca atgagtaaaa aaacaattat cgtcgcatgt
     4381 gcatcatcaa tgattacatc tacaatggca gctaataaaa ttcggaaaat agcaaaaaca
     4441 aatggattac cagaacctcg aattttgcaa ctaaaatttt cagaagttgc aggtgcttta
     4501 gaatcaaatc aagttgactt tatcgttcct acagggaaat tacaagaaaa tgtaaccaaa
     4561 ggaattaaag ttatacctgg aacaccattt gttactggag tgaatgaaga aaaatctgaa
     4621 tctttggtgt tagaagcatt gaaagagctt gactaggagg gtgaaaaatg aatattgtca
     4681 ttgatgcgtt aaactggatc tcaagtttgg gtccgatgat tatgatgcct atcattatct
     4741 tgattttagg attatttttt agaattaagc taaatacatt actaaaatca gcgttgactg
     4801 tggggattgg ttttgcgggt gtaaatatta ttattaattg gtttatttca caggtaggtc
     4861 catctgttca gttaatggtc aaaaattggg gtattcaaac aagtattatg gatgtcggct
     4921 ggccagctcg tgcagcagcc acttggtctt ttcctctagc agcagttatg gtatttatta
     4981 ttttgggtat taatgtgttg atgctgattt ttaaaaagac caatacagta atggtcgatt
     5041 tctggagtta taatcactat atttttgtag ctgctttggt ttattatgca acggataaaa
     5101 atattatatt ggcggtaatc ttcggggcga ttgatgcaat tattagtttt aaactcgctg
     5161 attggacaac gccgttagta gaggattact ttgaactaga aggggttaat ttcccgactg
     5221 cgaactcagt tggttgggca cccttggctt ggctacttaa caaattgtgg gaccgcattc
     5281 ctggattaaa taaggtagat gctcgtcctg aaaaaattca agagcggttt ggttttgttg
     5341 gtgagcctat gtttatggga tttgtcatcg gcttggttat tgggttatta gcaaaagaac
     5401 ctgttgataa ggtattgata gtgggaatga gtacttctgc aacaatggtt ctaacaccta
     5461 aaatgatgca aattttaatg gaaggattat taccctttgc taattctgtt aagaaattct
     5521 tgaatgtaaa atttccagga aacaatttta caatgggtgt ggatgctgcg ttgacagttg
     5581 ctaattcaag tgccattgct acgggtatta tcatggttcc gattacctta gtgttggctg
     5641 ctattttacc aggtaacaaa ctcttaccta tttcagatat tgcctaccaa gcaatgtggc
     5701 tatctgcatg gccggtcgcc ttttcaaaag gaaatattat tcgcggaata ctttccacca
     5761 cgattataac agcttgtgtg cttttaattg ctactagttt agccggcata catacacaat
     5821 tagctttggc tgggggattc caattaccag caggaatgac acaaatatct accgaagatg
     5881 caggtacaca cattattggt ttcattttaa tgaagattgc tgagtttatt tcaaatattt
     5941 tctaatattc aggagggcat acagtgaaaa tttcaaaaat tacagtctat aaagtaaaac
     6001 caagatggat ttttgtcaaa atatctacag atgaaggtat tgaaggctgg ggtgaaatga
     6061 tttctggaac taagactgaa acagttgtgg caggagctta tgagatagga aatcgattct
     6121 tagttggtca aaacccattt gcaattgaac gtctgtttca ggaaatgcat cgttcatttt
     6181 tccgaggtgg tcctattaat ggaacgattg tctctggact tgaaatggct ttatgggata
     6241 ttaaagggaa agccttaaat gttccagttt atgaattact aggtggagca gcccgtgata
     6301 aaatcaaagt atattcttgg attggtggag atcgtccttc tgaagtagta gagcaggcac
     6361 aagatcgagt agatcgtggg tttaccgccg tgaaaatgaa cgcgacagaa gaattacact
     6421 acattgattc ttatgaaaaa gtagctgaag tagtggaacg tgttgcttca attagagaac
     6481 gttttggaga tacattgagc attggtgtcg attttcatgg tcgtgttcac aaaccaatgg
     6541 ctaaggtttt agcaaaagca ctggaaccct atcatccaat gttcttagaa gaagtggttt
     6601 tgccggagaa tgaagaacat ttcagtgaag tagccaatgc agttgctgta ccactagcaa
     6661 cgggtgaacg tttgtataca agatggcaat ttaaatcaat tcttcaacag ggagcgattg
     6721 atatcattca accagatgta gctttatgtg gagggatttt agaaacaaga aaaattgctg
     6781 caatggctga agcttatgat atagcagttg ccccccatgc accatatgga ccgattgcgt
     6841 tagcagctac attacaagtt gattcttgta cacctaatgt gtttattcaa gaacaaagct
     6901 taggtattca ttacaacaaa gggtttgatt tgttagattt tgtaaagaat aaagagatat
     6961 tccaatacaa agatggcttc gtagatcttc cgacaaaacc aggtttaggg ttagaaatgg
     7021 acgaagatcg agtgaaagaa attgcacaag aaggtttagt ttggaccaat cctaagtgga
     7081 aaaattatga tggtacaatc gctgaatggt aggagatgag tgcatgatca atacggttac
     7141 aggcccaatc gaagctgagt ctttgggtac aacatttatt catgaacatt tatatgtcat
     7201 tcctaatgag ttaagaaagt atgaagatta taccttggat gatatttatc atagtattgc
     7261 ggaagttatg gcttttaaac aagctggtgg aaaaactatc gttgatttga caccaattaa
     7321 ttatggacga aatccgttag ctttgaaaat gatatctgaa gctaccggag tgaatgtagc
     7381 atttgttacg ggattccata aggaagagtt tcagccaaaa tggttgacaa cgctcaatga
     7441 tgatgagatc tttgagttgc tttattccga aataactcac ggagtcactt cacaaaacct
     7501 tttgccagga gcaatgaaag taggtacgag taaaaatgag gttacattgg ctgaacaacg
     7561 aatactagcc atagccggtc aagtacaaaa aaaaacaggg attcccatta ttacgcattg
     7621 tgatgggggg acgatgggac tggagcaaat taattgttta acaaaaaacg gtgcgaaagc
     7681 aagtaatatt tgtttgagtc atgtagattt aactaaagat tttcactatt tagctcaaat
     7741 ctgtgaaacg ggagcaagta tttgtatgga tcatattggc cgagagttgg cgaatcatga
     7801 tgcagaccgc gtccaactaa ttaaacgact ggttgatgcg ggttatgaaa atcagatttg
     7861 tctttcaggt gatatgggac gaaaaaaata tttgccaact tatggaggaa cgcctgggct
     7921 tacctatatt ttaacaagtt tgaaagaaga actattaaaa gtaatgagtg aaccaacttt
     7981 taataaaatg attgagatga atccaattaa tatactgagt aagtgaagtc aattgaatca
     8041 ttcagaagaa ctaatgaaat tagttgcttt cgcagggtta attcttctct ctgggtttgg
     8101 aaaagagggc ttagcttcgg attccctagt taaactaggg gttttatcag gttattgtac
     8161 tgattataag tacctgaatt attcatgaaa tactttttcc gtcgttttat aggcagttgg
     8221 gtcaataaaa agatcagaaa aacgaacgag agactctcat aacgtgccac taaataaaaa
     8281 tcgtacgtta ttttaaaaaa tgggttgatt ctaggcagta ctttgtcggt ctatttttag
     8341 aaaaagaggt tctttgtcaa acatacatga attcgcaagc tcattcaccc tgaaggatga
     8401 aaggcagaaa ctgtagtata ctaatggtgg aaaggaaaaa aagccgccag caatttggaa
     8461 aagaacgtta tgtcgattgt caaactggct tgctttattt ctcgagaacc ctgaaatata
     8521 gccaatgagc atgtgtataa aaaattgatt ttatatgaaa taaagcactc ctttcctaaa
     8581 attattggaa aggagtgcgt tttttatgga aattatttag gttctttgtc aattgctatt
     8641 gttttgctca aatcgtatgt taggaaatgc ttcggcattt ccttttttgc gttattaaat
     8701 gatttcacag atgttctgtt gaatcaggac acgcatccgg agatgacgaa aagtccgaaa
     8761 tccaaaagca attcttttga ttactttgat gaggttattc tttccctcca attttccgtt
     8821 tgaataggga tattttaagg agtttgtgat tgcttctgtg tgctttttca gataacaaat
     8881 tgccttttta aacccatcat tcaaggaatg tggcaatgat tcaatgagat tgaaaaattc
     8941 agtaaaatct tttgcatcaa aagcagctaa taactcttga tagacttcat agctttgtcg
     9001 tagttggtta tctatagaga gtaggtaatc aagcacctcc gactccgtca tgtatttccc
     9061 ttggaagagg ggaaattgct taaaa
//
LOCUS       DAZUMK010000105         9073 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000105 DAZUMK010000000
VERSION     DAZUMK010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.107"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            14..1837
                     /locus_tag="ACG808_002267"
     CDS             14..1837
                     /locus_tag="ACG808_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS5047869.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(1979..2119)
                     /locus_tag="ACG808_002268"
                     /pseudo
     CDS             complement(1979..2119)
                     /locus_tag="ACG808_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            2640..2903
                     /locus_tag="ACG808_002269"
     CDS             2640..2903
                     /locus_tag="ACG808_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS5047870.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            2903..3169
                     /locus_tag="ACG808_002270"
     CDS             2903..3169
                     /locus_tag="ACG808_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFS5047871.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            3370..3858
                     /locus_tag="ACG808_002271"
     CDS             3370..3858
                     /locus_tag="ACG808_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFS5047872.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(4399..5070)
                     /locus_tag="ACG808_002272"
     CDS             complement(4399..5070)
                     /locus_tag="ACG808_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5047873.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(5067..5984)
                     /locus_tag="ACG808_002273"
     CDS             complement(5067..5984)
                     /locus_tag="ACG808_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS5047874.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            complement(5981..6622)
                     /locus_tag="ACG808_002274"
     CDS             complement(5981..6622)
                     /locus_tag="ACG808_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5047875.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            complement(6626..7207)
                     /locus_tag="ACG808_002275"
                     /pseudo
     CDS             complement(6626..7207)
                     /locus_tag="ACG808_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
     gene            7365..7769
                     /gene="tnpA"
                     /locus_tag="ACG808_002276"
     CDS             7365..7769
                     /gene="tnpA"
                     /locus_tag="ACG808_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFS5047876.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
     gene            7786..8934
                     /gene="tnpB"
                     /locus_tag="ACG808_002277"
     CDS             7786..8934
                     /gene="tnpB"
                     /locus_tag="ACG808_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="HFS5047877.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT         2823 a         1697 c         1861 g         2692 t
ORIGIN      
        1 aggagtttag aaattggatt acttaaaaag gttgttagta ggtaagcctt taaaatctgc
       61 tgaaaatgat gagcacaaat taacccgatt tgctgctttg gcattattgt cgtctgatgc
      121 gttatcatct attgcatatg gtacggaaca aatcgttgtc gttttagtcg ctttgtctgc
      181 tgcagccatc tggtattcgc tacccattgc cgcttttgtt attattttat tgatttcatt
      241 aacgctatca tatcgtcaaa tcatccatgc ttatcctcat ggcgggggag catatgtcgt
      301 cagcagtgag aatcttggaa agaatgctgg cttgatttcc ggtggctctt tattgatcga
      361 ctacatgttg acagtagcag tatctgtctc agcaggtgct gaagcgatca catcagcggt
      421 gccagcactc tacggtcatc aagtcgctat ttctgtgaca atcgttttgc tactgatgat
      481 gctgaatctt cgtgggctga gggaatctgc atcgttccta ttatttccgg tgtacacatt
      541 tatccttgtg atctcgttac tgatcgttgt tggtttatat aatatcgtga caggtgctgt
      601 gccattgcaa gctactgcac ttccaggagc ggctgtgccg ggtgtttcta tcgctttgat
      661 tttacgagca ttttcttctg gttcttcctc tttaactggg gtggaggcga ttagtaatgc
      721 tgtaccattc tttaagaagc caagagcaaa aaatgccgct gctacattga cgatgatggc
      781 attgatctta ggtttcttct tcgtggggat cacatttatc aactattggt atgggatcgt
      841 tcctgaaaaa gaagtcactg ttttgtctca aattggtaaa gctgtctttg gacatggtat
      901 tttgtattac gtccttcaat ttgctacagc gctgatttta gcagttgctg ctaacaccgg
      961 tttttctgct tttccagttc ttgcatacaa tctggcaaaa gataaattca tgcctcacat
     1021 gtatcaagat cgtggggatc gactaggcta ttccaatggg attatcacac tagcgctagg
     1081 ttcgatcgta ttgctgttta ttttccatgg ctcaacagaa cgcttgattc cgttatactc
     1141 tatcggcgtg ttcattccat ttgctttatc tcaaacaggg atggtcgtca aatggaaaaa
     1201 agaaggaaaa agatggttaa gcaaatcgat tgccaatatt acgggtgcgt tcatctcata
     1261 tgcaatcatt gcgattttgt ttgtctatcg gttaggggac atttggcctt tcttcatcat
     1321 tatgccaatc gttatgttca ttttttataa gatccatgat cattataaaa aagtagcaga
     1381 acagcttcgt ttagaaaatg atgcaaaact acatgattat gacgggaata ccgttcttgt
     1441 attagtcggg aatgtgacac gggtaaatat cggtgcgttg aattacgcac gttcgatcgg
     1501 tgattatgtc gttgcgatgc atgtttcgct agacgaagat gtcgaaaaag aaaaagaaat
     1561 cgaagcagaa tttaaaaagc atttcccaga tgtccgtttt tcaatcgtcc actcttctta
     1621 tcgctcgatt gaaaatccaa ttatccgtta tgtagatatc gtaagtaaaa atgcagcgaa
     1681 acaaaactat acaactactg tgttgattcc tcaatttgta ccaaacagac gctggcagaa
     1741 tattctccat aaccaaacaa gtttgcggtt aagattgcga ttgtcttggc gtgaaaatat
     1801 cgttgtcagc acctatagct accatttgaa gaaataaacc aagttttaaa atcaaaaaca
     1861 gaccctctag ctcttaattg aagctagagg gtctgttttg tcttattctc agctaaaggt
     1921 gccctctata agccattaaa gatgggaaca gcgtatgaaa tgttattatt cttcctattt
     1981 agctgtaaaa cattttgatt ttgatgtctt gatcataatt tccgaatcct ttgccaaata
     2041 atgtacagcc tcctatattt tcagcttctt cctttacctc gactcttaag tgccatgtgt
     2101 cttcataagt aggaatctca gcctcgtcaa gtgaccattt tttgttacca taactcagtg
     2161 ccatttttaa ttggtgattc acaaacccaa cggtctaagc aagtatcaaa tcgatagtga
     2221 acatgaatga caggagacga aagaggcaat catttttgag gcagtagaag acttgacggc
     2281 caaaatcagt tttgaataac cgttacaaat ctttcgtgcg tatcctcagg ttattcaaga
     2341 tatcaatcaa gtcacactgc aacgaggaac cttcgtgtat tggtgttgtt gcatagactc
     2401 acaggctttg gattcgacct ttgctaaaga tgatacgttg ttaccaatta ctttacgttt
     2461 ggctaccgct agtcgtaaag gagtaagaag tgagcgtgga caaagatcct ccctaaaaag
     2521 gggtcttttt ctctatgtaa aaaagaggaa aagtttgctg aatttaagtg ctttattgta
     2581 tttttttgac gtacttaatc aagtacgcta taattatatc agaaaaagga ggagagacaa
     2641 tggctaatac aacgatgaat cctagcactg ctcgtaaaaa tttttatcag ttattgaaag
     2701 aggtaaatga aaatcatact gaaatcgaga ttattagtga tcgtagtgga aacaatgctg
     2761 tattgattgg gctggaagac tggagagcga ttcaagaaac cctttttctt gagcaaacag
     2821 gtactttaga taaagtacga gaccgcgaaa aagacgacag tagctttacg aatatcgatg
     2881 atattgattg ggaagctctt tgatgagtaa ctatacagtc gcaatcaaaa actctgcgaa
     2941 agtggattta agaaaaatca agcaaacgaa tttaaagaaa caatttgaag aagtgattca
     3001 gacattgaag gaagatccat atatgcccac gcagtctttt gaaaagctga gacctacaca
     3061 tgaaggcaga tactcacgca ggctgaatag acagcatcga gtggtttaca aagttgatga
     3121 ggaaaataaa gttgtagaaa tttattctgc ctggacacat tatgagtgaa gtgagaagag
     3181 ccaacctttt tagatagtta acaaagagtc tccccaaaaa ggagactctt tttttgttct
     3241 aaaaccaacc ttcttggatt tattgcatga tttctttaat ttgttcctat atctccacct
     3301 ttgaagggtc attgtttgag tttaggaatg gaatgttgac gggtttatgt aaaacaagga
     3361 ggaagaagtg tgacaaggaa aatcagtaaa attggagcat cgttgttagt gttggcagtg
     3421 gcaacgacca tcgattatcg ccaaagtgag gcgaagacaa ttggaaatgt cgtttatcga
     3481 gaaacagcca aaagtatgtt gaaaaaagca atggacaatc aaccggagtc gtcttattgg
     3541 ttcccagagg attcgttggc atggtctttt ggaaaagata cagatacgaa atacaatacc
     3601 agtgtcgttc ctttagcaga gcgggtatcc aaagcgacat tgccgaacat gaatgtcact
     3661 caaacggaag aagttaaagt cgtggcgctt tcggccatga atagcagtat caacggcaat
     3721 gcatcacgag ggatcgatat cttttacgcc aatgtctttt cttattggca atatatcgat
     3781 caattggttt acgggggtgc tcttctggtg aaggatcatc gtatcgccaa gtctagttgc
     3841 gacggatgca gtccataaga atcgtgtacc ggttttagga accgcaaacc tcccatggcg
     3901 agaagctgga atggctagat atgtttttag ccaaggatga ggatggcaag ttcccgattg
     3961 tataaaagat gatcgaagtt accgatacgg cagtcaccag ttttgatgat gcagctgaag
     4021 gcatcgaaca atcgaggact gatggaaaag gcttaaatga aagccacgcc aaaggatgta
     4081 agaattgatg cccaattcaa agaacaagca gacgagtatt tagagatcat gtggtatgaa
     4141 ttccatgacg agtgatggga aaatggattg gcaaaatgcc ttagcggata agaaaaaaac
     4201 ttagtcggtg gatgcagata tgaacccgtt ggcagtatga agttggagta tacggaatgg
     4261 ataaatagct aagaaaagca actctttgcc cataaggagg gaagaatcag agtggattat
     4321 tgccttaaaa atgacagaac taaaaaagcc tttgcgacca acaaatggtc gcagaagctt
     4381 ttgtttttta caataacatc agcgtgatgt acggctggct ggatctagcc gtctttccaa
     4441 aatacctaaa agccagtccg tgatgatcgc cattaatgca gttggcaatg cacctgctaa
     4501 aataatcgat gtgccctctg ttgcattggt tcctcggata atgatatctc caagaccacc
     4561 agcaccgacg aatgcaccga tcgcggttat cccgatggca acaactaacg cgtttcttat
     4621 gcctgccatg ataacagaaa cagagagtgg taactctacc atgaatagtc gttgtctagc
     4681 agtcatcccc atgcctttgc cgacatcaag gatgtttttg tctacttgga tcatcccggt
     4741 atacgtattc ttgatgatcg gtaagaggga atacaaaaat acagtaacaa tcacgacatt
     4801 gactcccagg cctaatccaa tgattaaaat ggagatcatg gctagagaag ggatcgtctg
     4861 gatgatattc gccaaacgaa tcacccagtt tgctaattta cgtctcctag aaatcatgat
     4921 cccaaccgga atcccgacaa ctgctgcaaa gaggacacca tagatagaaa tcaggaagtg
     4981 acggataaat tgtgcaaaga tataggagcc attttcctgg aaataataga aaaattgttg
     5041 aaagacattc attttatcca attgcttcac ttcccttcaa aatagtggtt ttcttgtaag
     5101 aaacgttgtg ctactactga tggttccact agattattat ctgcttcgta gttcaattcc
     5161 tgcatttttt ccgtactgat ggtatgacct aacttattta aggcttcttt taaatgaggc
     5221 gtatcagcta atatcttgtt gtctacaact ggagcagcat catatggtgg gaagaattga
     5281 cgatcatctt ccaacatgac tagatcataa ctggcgatcc gtccatccgt agagtagcca
     5341 agaacaatat ccatttttcc tgcttcgact gcgtcataaa caagtccaat ctgcataggt
     5401 aatatcgaat tgaaggagaa gccataagtt tcttggaagc cgtcataacc gtctccttta
     5461 cgattgatcc aagaagtatc tacaccagct actaattcat ccgctacatt tttcagatcg
     5521 ctgactttag atagatggta tttttgtgcg gtgtcttttc gaacaagaaa gacatacgta
     5581 ttttcaaaac cataggacgg gaaccacgtt tgaccgtatc ttttctcaaa ttcatctttc
     5641 acggtagcaa acgctttttt cggatctttt tcaggaggaa ggttcaatgt agtcgttaaa
     5701 tctgttcctg tgtagcgagc cgctgaaatt gaagcatctc cgttcatcat cgcttgatgg
     5761 ttgatagttg tagtagccag attgttgatg attgcggtat ttttatctgt ataatgttca
     5821 accattccgg caactagact tgctaagatc tgtgcttctg aagtgattcc acctgtgatc
     5881 gaaatggtgt cttcatctgt attgctggca agaccaggga aggaacaact cgcaagtata
     5941 agaagagaga aaagaaccat ggtcattttt agtatacgtt tcattcttca ttctcctctc
     6001 taagtgcgat cggtgtcagc cggtgttcca agagacctag aagccaatca gccaatagtg
     6061 ctaaaattgt tacagggatc gtacctgcca aaatgagatc tggttggtaa ttattcagtc
     6121 cactaaaaat caaatcgcct aaaccaccag cgccgatata agaggcaagt gtagcccatg
     6181 caatcacgta aacggcagct aaacgaatac cagccatgat cgttggcata gcaatgggca
     6241 attcaacgga aaaaatggat tggaactcag tcatccccat gccttttgcc acatcccgat
     6301 aattccaatc gacatttttc atcccaatgt acgtattacg taaaatcggc aataatgaat
     6361 aaataaagag tgcgataatc gcaggtaatt tacctacgcc aaatagcggg atcatcaatg
     6421 ccaataaagc aagagaaggg attgtctgca aggcactggt taagccaatc acaatcgtgg
     6481 cagcttttgg aaaacgtgtc agtaagacac caagcggtac agcaatgatg ataccaatga
     6541 tcaaagcaag tcctgaaata taaagatgtt caaaaccttt tgagagaatt tctgaaccgt
     6601 gctgatgaaa aaactgctgc ataggtcaga cctccttaga ttcttcagga gtagctgact
     6661 ctgcaacatc agtagttgcg ggatcttctc cccaaatcac atcgtagacg atatccacta
     6721 atgatgcacg agtcaggata cctacgactt tgttctgttc atcaacgacc ggcacgtatt
     6781 tcagcccgcg ttttaaaatg cgttgcaagg tatcacgaag ataggcggat tcttttacgt
     6841 aaaaaacatg tttattcaaa atgtcgccga cactggatac ttttcctcgt tttttattca
     6901 acgtttctac atcgataaat ccttttaata cattcgaatt atcggtaact aacaacgtat
     6961 ctacacgttt ttccctcatc agtttgattg cttcttgtaa tgatttttcc ggtgtaatcg
     7021 tgattgctgt atttagcata acttcgccga ctcttgtcgc atttggcttg gcttgtagta
     7081 ggcgatcttc tccaatcaac tcttctacga aatcgtttgc aggatgacgt aaaatatttt
     7141 ctggtgtatc aaattggatc acacggcctt cactcatgat ggcgatacgg cttgctagtt
     7201 taacagaatt aggcagaagt cgcccatcct ctataggtgg gtgatgaatg ccgttcggta
     7261 tgagggttac cgttggtaac tcgaagaccg acaactaagg cgtattgcct ttttattttg
     7321 ttccatgtta caatcagtct atacaagaaa tgagttgata taacatggaa ttagatacaa
     7381 ataatcattc agtatttctt ctttattacc accttgtttt agtaacgaaa tatcgtagac
     7441 aagtgattga tgatgaaata tctgattatg ccaaaactac ttttgaaaga atttcagaat
     7501 cgtatcacat tactttagtg gagtggaatc atgataaaga ccacgttcat attatgttca
     7561 aagctcaacc taaaacagaa ttgacaaaat tcatcaatgc ctataaaagt gccagctcaa
     7621 gattgataaa acgtgacttt ccaagagtca aacaatttct ttggaaagaa atgttctggt
     7681 ctaaaagttt ttgtcttctg acaactggtg gcgcaccaat tgatgtgatt aagaagtata
     7741 tccaaaatca aggaaacaat cataaataga aagtaggtga actttatgga acaactaaaa
     7801 gcatacaaat tcagaatcta tccaacagag gaacaagaaa tcttctttgc caaatctttt
     7861 ggttgtgtcc gtaaggtcta caatctaatg cttgatgacc gaaaaaaggc gtatgaagaa
     7921 gttaaaaatg attcttctaa aaaaatgact tttccgacac cagctaaata caagaaagaa
     7981 tttccgtttt tgaaagaaat tgatagcctt gctttagcca atgcccaact taatttagat
     8041 aaagcataca agaatttttt tcgggataaa tccgttggat ttccacgttt caaaagtaag
     8101 aaaaatcctg ttcagagtta tacaacgaat aatcaaaatg gtacagttgc tttgattgat
     8161 agtaaattca tcaaagttcc taaattgaag tcattagtaa gaattaagct tcatagacaa
     8221 ccaaaaggga ttatcaaatc tgctacaata tcacgtcatt ctagcggtaa atactatatt
     8281 tctttgttgt gtaaagaaga aattatcgaa ttacctaaaa ctaattccac aattggtatt
     8341 gacttaggta ttatggactt tgcgattctt tctgatggac aaaaaattga taataataaa
     8401 ttcacgtcca aaatggaaaa gaaactaaag cgtgaacaac gcaaattgtc taggcgtgct
     8461 ttattggcta aaaataaagg taccaatcta tttgaagcta aaaattatca aaaacaaaaa
     8521 cgcaaagtag ctagattgca tgaaaaagta atgaatcaac gtactgattt tctgaataag
     8581 ttgagtacag aaatgatcaa aaatcacgat attatctgta ttgaagactt aaacacaaaa
     8641 ggtatgttgc gaaatcataa attagctaaa agtatttctg atgtttcttg gtcaaagttt
     8701 gtgactaaat tacaatacaa ggctgactgg tatggacgaa aaatcatcaa agtagataaa
     8761 tggtttccgt ctagtcagat ttgttcagag tgcggacata aagatggcaa gaaatctctt
     8821 gaaattcgag aatggacttg tcctgtttgt catactcatc atgatcgtga tatcaatgct
     8881 agtatcaata ttctgaccga aggtctaaga ctacattcta tgggattggc ttagatccaa
     8941 tcatcaagaa ccgtaggaac tacggggata gcttggtaaa taagagacac cgctgttagt
     9001 aaagaaatac gctatcaagt atgctctatt cccaagaagc tcccacttca agcgttaaga
     9061 accgtcaggt tta
//
LOCUS       DAZUMK010000106         9026 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000106 DAZUMK010000000
VERSION     DAZUMK010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.108"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            130..354
                     /gene="nrdH"
                     /locus_tag="ACG808_002278"
     CDS             130..354
                     /gene="nrdH"
                     /locus_tag="ACG808_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFS5047878.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            513..2672
                     /gene="nrdE"
                     /locus_tag="ACG808_002279"
     CDS             513..2672
                     /gene="nrdE"
                     /locus_tag="ACG808_002279"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFS5047879.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            2722..3687
                     /gene="nrdF"
                     /locus_tag="ACG808_002280"
     CDS             2722..3687
                     /gene="nrdF"
                     /locus_tag="ACG808_002280"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFS5047880.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(3776..4915)
                     /locus_tag="ACG808_002281"
     CDS             complement(3776..4915)
                     /locus_tag="ACG808_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS5047881.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(5224..5937)
                     /locus_tag="ACG808_002282"
     CDS             complement(5224..5937)
                     /locus_tag="ACG808_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFS5047882.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(6191..6892)
                     /gene="nagB"
                     /locus_tag="ACG808_002283"
     CDS             complement(6191..6892)
                     /gene="nagB"
                     /locus_tag="ACG808_002283"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFS5047883.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            7126..8658
                     /locus_tag="ACG808_002284"
     CDS             7126..8658
                     /locus_tag="ACG808_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS5047884.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(8849..>9026)
                     /locus_tag="ACG808_002285"
     CDS             complement(8849..>9026)
                     /locus_tag="ACG808_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5047885.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         3015 a         1757 c         1586 g         2668 t
ORIGIN      
        1 aaaaacacaa aacttagtat tccacagttt ccacaacaca atatgtagtg tttgttgttt
       61 gactttttta ctaagagttt tatactatct attatagata ataagaatgt gttagaggag
      121 gcactcacca tgaacgtaaa aatcttttct aaaaataatt gtatgcaatg taagatggtc
      181 aaacggtttt taacagaaaa tcaaattgct tttgaagaag tcaatatcga tgaacagcct
      241 gaagcagtag actggttgaa agcacaagga ttccaaagtg ttcctgtcat tacttctgat
      301 gcagaaacag ttatcggatt ccgtcctgat cagttgcgta aacttgccag ctaacatgac
      361 taaaagaatc aaaacgtgat acatggattt gaagggcttg agacagcacc agtttttgtc
      421 gcaaacttcc aaatcctttt ttaaagctta aaaacaatgc aatgatttgc aatcaagcat
      481 catcaaatac gaaatgaagg ttgggcgtac gtatgagtct taaaaattta caagatgtca
      541 gttatttcaa gctgaataat gaaatcaatc ggccagtcaa tggtcaaatc cctttgaata
      601 aggataaaga agctttagtt gccttttttg aggaaaatgt caaaccaaat acgatgactt
      661 ttccatctat tatggaaaaa atcaattact tgatcgatca agaatatata gaagcagaat
      721 tcattcaatt gtattcacca tcatttattg aaaagctata tcaatttttg gacgagcaac
      781 atttttcttt caaatctttt atggctgctt acaaatttta ttctcaatat gcgttgaaaa
      841 ccaacgacgg ttccgcttat ctggaaactt atgaagatcg agtagccttc aatgcgcttt
      901 actttgcgaa cggggatgaa gaattagcac ttgctcttgc agacgaaatg atccatcaac
      961 gatatcagcc agctacacct tctttcttga atgctggacg taaacgtcgg ggcgaattgg
     1021 tgtcttgttt cttgactcaa gtcactgacg atatgaactc tatcgggcgc tcaatcaact
     1081 ctgctttgca attgtcccgt attggtggcg gcgtagggat tactttatca aatcttcgtg
     1141 aagccggcgc tcctatcaaa ggatatgagg gtgctgcaag tggtgtcgtt ccagtcatga
     1201 aattattcga agatagtttc agctattcta atcaattagg tcagcgtcaa ggagctggcg
     1261 tggtttattt gaacgttttc catccagaca ttattgcttt cctttcagca aaaaaagaaa
     1321 atgcagatga aaagattcgc gtaaaaactc tttctcttgg cgtgatcgta cctgataaat
     1381 tttatgaatt gacacgtaaa aacgaagata tgtatttatt tagtccatat agtgtcgaaa
     1441 aagaatatgg tgtcccattc tcttatgtaa atattacagc tgagtatgat catctagttg
     1501 caaatccaaa cattcgcaaa caaaaaatca aagcgcgtga tcttgaaaat gaaatctcaa
     1561 aattacaaca agaatctggc tatccttacg taatcaatat tgatacagcg aaccgcgaga
     1621 atccaatcga tggcaagatc atcatgagta atttatgttc agagatttta caagttcaaa
     1681 cgccttctgt gattaacggc aaacaggaat atgaagtatt aggaacagat atctcatgta
     1741 acttaggctc gacaaatatc gtgaatttga tgcaaagccc taactttggt caatctgtgc
     1801 gtacgatgac tcgtgcattg acatttatta cagatgcatc tgatatcgat gtcgtaccac
     1861 cgatccaaaa cggaaataag ctgaatcata cgattggatt aggtgccatg ggcttacaca
     1921 cttatcttgc aaaagaacaa atggaatatg gctcagagga ttcattagac tttacaaata
     1981 tttacttcat gctgttgaat tattggacac ttgttgaaag caacaatatc gctcgtgaaa
     2041 gaaaacaagt tttccataat tttgaaaaat cagcttatgc tgacggcact tatttcgaaa
     2101 aatacgtaac tggagaattc cagccaaaat cagaaaaagt caaagcattg tttgaaggca
     2161 tttttatccc aactgctgaa gattggaatg ctttgaaaca agcagtaatg aaagatggac
     2221 tgtatcatca aaatcgcttg gcagtagcac caaatggctc aatttcatat atcaacgata
     2281 cgagtgcaag tattcatcct atcactcgca tgatcgaaga acgccaagag aagaaaatcg
     2341 gtaaaattta ttacccagcg ccttatctat ccaatgagac gattccgtat tacacttctg
     2401 cttatgatat ggacatgcgt aaagtaatcg atgtttatgc aacggcacaa caacacgtcg
     2461 atcaaggcat gagtttgact ttattcatgc gttctgaaat cccagaaggc atttatgaat
     2521 ggaagacaac aacgaagcaa acgacacgtg atttgaatat tttaagacat tacgctttcc
     2581 acaaaggaat caaatccatt tattacgtac gtacctttac tgatgatgca gaagaaatcg
     2641 gaagcaatca atgtgaaagc tgcgtgatct aatatcatag atagattcgc ttattcatat
     2701 tttaattaaa ggagcattct catgtcagaa acatattatg aagcaataaa ctggaatgaa
     2761 attgaagata tcattgataa atccacttgg gagaagctga cagagcaatt ttggctagat
     2821 acacgtatcc cactttcaaa tgacctagat gattggcgta cattgtctca attggaaaaa
     2881 gatacagtag gacatgtatt tggcggtttg acactgctag atactgtcca atctgaaagt
     2941 ggcatggatc agttgcgtaa agatgtccgc actccccatg aagaagccgt tttgaacaat
     3001 attcaattca tggaatccgt tcatgccaaa agttattctt ctatttttag tacactgaat
     3061 acgaaaaaag aaatcgaaga aatttttgac tggacaaata cgaatcctta tctgcaaaaa
     3121 aaagctgagc gtatcaatga aatctacaaa cacggtacac cgctagaaaa gaaaattgcc
     3181 agcgtctttt tagaaacatt cttgttctat tcaggttttt atactcctct ttattattta
     3241 ggaaacaaca aattggctaa cgtagcagag atcatcaaac taatcatccg agatgaatct
     3301 gtacatggaa cctacatcgg ttacaaattc caattaggat tcaacgagtt acctgaagca
     3361 gaacaagaaa cgttaaaaga ctggatgtac aatcttcttt atgagcttta cgaaaacgaa
     3421 gaacgttata cagaagaatt atacgatcca atcggctgga cagaagaagt caagacattc
     3481 ctacgctata atgccaacaa agcattgatg aacctaggga tggatccact ctttccagat
     3541 actgccaatg atgtcaaccc aatcgtaatg aacgggattt caacaggcac aagcaaccat
     3601 gacttcttct cacaagtcgg aaacggctat ttgttaggtc atgtagaagc gatgaaagac
     3661 gacgattatt tgattggatt ggaataagga tttattttaa ttcaaaaatt tttcgaaaaa
     3721 taaaaagagt ttagcgtaca gatattttaa atgtacactg aactcttttt tatgcttatt
     3781 cacttgtttc ttccagcact tttggactgt ctaacttttc cgaaacatgt tccattcttc
     3841 tcaataaata gattttccgg atatttaata ttatcgtctt aatcactgca tagacaggta
     3901 tgcaaatcag catgcctaat attcctgcga cacttcctgc tccaatcaat accaagatga
     3961 tcgtcaaagg atggatattc attgatttcc caaataacaa cggcttgatc aaatttccat
     4021 ctaattgttg gatcactaaa atcgacaacg ccatatacaa agcttgaaat ggagagataa
     4081 acaaaccgac aatgattgct ggtaccgctc caataaaagg gccgacatac gggataatat
     4141 tcgtgattcc gcaaatgacc gcaagcaaca agctataagg ctgatcgaat attctcattg
     4201 ccaaaaagct cattacacca ataatcaaag catctaagat cgtactgctg atataggcag
     4261 ataatgtttc gtttaattct tttaccgttt ggcgtaactc tttacgaatc gctttcggaa
     4321 agaagtgaga caacgcatca ataaatttgt gcccatcctt gaacataaat agcaaaataa
     4381 atggaacggt aaataacaaa ataaaaaatt gcatcataaa tgaaatgatt tttgagacac
     4441 ttgcagataa actgactaat atgatattga acaaattact aagagataaa tttgctgatg
     4501 ctaattgttc ttccaaattg aaatttttaa actcttcccg ctgcgccaat tcatttaacc
     4561 atttgcttgt ttcatctgca tacattggca agctctgggt caattgaatt gtctgttcca
     4621 ttaactgagg cactacattc atgacagtta aaacaactaa aatgattacg accaaaaacg
     4681 tcaataaaaa accgaacact ctcgggatcc gatgcttttc gagaaaaaca acgattggat
     4741 cgaacatata atagataaaa ccagcaacaa gcaatggtaa aaaaattgcc gaaagcattt
     4801 tcaatattgg actaaaaata ttcggcattt gtaaaataaa gtacacgccg atgatcgtga
     4861 gtactagctc cactgtccag aaaaataatt ttgattgttt aaaacgtgat tgcatacatc
     4921 tcctccaagg tcctttgata cttctattct atcatttaac aaagagttac gcgctgagat
     4981 tttcatttgc ttaaaaaaaa caaaaaaaat aaacatattt taatgagtag acgagtactt
     5041 aaacaaaaac acggtttgga gtagtgacaa aaattatact gcagtccctt tttccaaata
     5101 aacggtgttc aaaagcttgc atcttcgtaa taagcccagc caaacgaaaa atataaggaa
     5161 ctatttttat ttggctgggc ttattacttg atgtaggaca tctattttac aacctcagat
     5221 catttattgc gcaatactga ctaaatgcaa ctttgtgata tttttgtgta cggacaactc
     5281 ctcgatcaca tcggcataag tgagcccttt tggaagatca atcgtataga tatttttgta
     5341 cactcggtag tcatcaacga attttacgtc aaaattcaca tcttcgatta cgatattttt
     5401 ctcttcaaaa taatcggtaa gaaaatcttt ggtttctttt ctatggataa attggatttc
     5461 taacttttta gtcgtaggta catggatcac gcgtttcact actgtgagtg caatagcaat
     5521 cccgataaaa ctgatgatcg caatggaata gtaacccatg ccgatggcga tccctaatcc
     5581 agcaattgcc cataaagaag cggcagtcgt aagtcccgtc accgattgtt tcgtgacgat
     5641 gatcgttccc gcgcctaaga aaccgacacc actcaccact tgagcaatca gccttgcttc
     5701 atctgaacga atcgttccag caagctgtgg atactctaaa gcatctctta atgcaccact
     5761 agcgatctca acttggatca atgcaatgat acatgctccc atacaaacta aaatatgcgt
     5821 acgcatacct gctggacgat ttttatattg tcgttcaaaa ccgattgctc cgccaaatag
     5881 catagcgatc cctaaacgta aaataatctc tggaattgtc aataccatat cattcatcta
     5941 aacaccctcc agtccaatat atcatgtctg aaaggagcag acaaaaaaag tgcaattttt
     6001 acctcttacg aaaaaaggac tgtgacaaaa tttttgtcat agtccttttt atgaataaac
     6061 ggtgttcaat cagctgaccc tcggtattac tcaacaaacg gcaaaatatg gggagctatt
     6121 tttcctttgt cttctctaat actcaggtca aaacgctgat ttcacaacct ctttagccta
     6181 ctattgaaaa ttacaactta cttgctgctt cttcatcgat gatcacaaca acattgtcgt
     6241 gattttgcaa tacacttgct ggcaaatgat tcgttacagg tccttcaatc atccctttga
     6301 ttgcatcagc tttgctttcg ccaaatgcca tcaagacgat ttctttactt ttcataattg
     6361 aaccgattcc cattgaaaca gcttttgttg gtacatcttc tgctttatcg aagaatcgtt
     6421 tgtttgcatt gattgttgat tctgttaagt caactactga agtcgttccg tcaaatgaag
     6481 caccaggttc attgaaaccg atatgtccat tttgcccgat tcctaagatt tggatatcga
     6541 ttggatgtgc atcgatgatt tgatcatact ttttgcatgc tgcttccaga tcttctgctt
     6601 ttccatctgg tacaaacgtt tctttgaatg gttttttatc aaataattgg acatccatga
     6661 aatgacggta gctttgttca tctgaaccgc ctagtccaac gtactcatct aagtttacag
     6721 aagtcatttc actgaaatcc aaatcgcttg aagtcatttc tttatataaa gtaactggtg
     6781 tgcttcctgt tgccaatcct aatacttttg cacctttgtt cataccttct ttgatgattt
     6841 caaatgcttt ttttccgcct tcttcagcgt tttttacacg aatgatttcc atagtgattg
     6901 ctcctttata ttttggtata gcccaattat agcattcttt ttttcttttt acaataatta
     6961 gtatagacca aattgcattt ttttcatttt cttgacaata aataggaatc cgtgttaggt
     7021 ttcataagat ggttcaatca aacaatccat gttgcatgaa aaccaagcaa gtgattcact
     7081 gtggacgctt tatgtctgca gtttttttat gaaaaaggag ttattatgag tatactgaca
     7141 ttaaacaaca ttacccagca atttggcgac aaaatcctct atgaaggtgt cgatctgcaa
     7201 ttaaatgctg gcgaacatct tgggctgatt ggacaaaacg gagccggtaa aagtacattg
     7261 attaaaatca tcactggtga gatcttacca gatgacggac agatccattg gcaaaaaaat
     7321 attcacaaag gttacctcga tcaatatgtg gaagtcgatg aaacattaac aatcgaagaa
     7381 tttctaaaaa ctgcgtacgc aaaagaatac gaaaaggaag aaaaaattgc ttttctttac
     7441 caaacatata gcgagacctt cgatgattcc ttgctggaaa aagctgggaa actccaaacg
     7501 gaattagacc aaggagtgtt ttatcaaatc gacaccttag ttgctgagat gagcagcggg
     7561 ttggggatcg atgtcttagg tttagattca ctgttgaaaa atctaagtgg tggacaacgt
     7621 tcaaaggtga ttttagcaaa actactttta gaaaacccag atgttctgat cttagatgaa
     7681 ccaaccaatc acttagatga tcagcatatc caatggctga cgcagtttct tcaggatttt
     7741 gacggtgcgt atatcgtgat ttcccatgac caagaatttc ttgatcagat caccacccat
     7801 atcgcagaca tcgagtttgg aaagattact aagtatactg gccatctaaa acaagcattg
     7861 aaacaaaagg aacaaaatcg cgaaagctat ttgcgccaat atcatgctca acaaaaacaa
     7921 atcgaaaaga ccgaagctta tattcgaaaa tacaaagccg gttcccgatc aacgatggca
     7981 aaaagccgac aaaaacaatt agataaaatc gaacggctga cgcctccagc ttcagcaagt
     8041 aaagctgtct ttgattttcc attctcgcct attgtcacga cattagcagt ggaaacaact
     8101 gatttagtca tcggatatga tcgaccgcta cttgcaccca tcaatttgac catccgcttt
     8161 ggtgaaaata ttgccattcg aggcttcaac gggattggaa aatctacttt attaaaaaca
     8221 ctgatcggtg agattgaaaa aataagtggc gatttccact tcccagataa cacaaaaatc
     8281 aattactttt cacaagatct gtactgggaa aatccattag aaacaccact acaatattta
     8341 agcagtcaat ttccaaaagc gactatcaaa gaattacgtc gccaactagc taaagcaggg
     8401 ttagttaatc aattagcaag tgaacctctc actacattaa gtggtgggga acaaactaag
     8461 gtgaaacttg cacagttgac aatgaaccaa ggtaatttac tgattctcga tgaaccgacg
     8521 aaccatatcg atcaagaaac gaaagaaagc ttacaagacg ggatacagaa atttccagga
     8581 acagtaatca tcgtctctca tgaacaagaa ttttatcaag atcttgtgca acgagtcatt
     8641 gagattgaac caaaataaac gtttgattga atagttgaaa aaaaggagct gtgacaaaag
     8701 ttttgtcaca actccttctt ctgaataaat ggtggccaat tagctgacct tcggtattag
     8761 ctcaaaaaac ggcaaaatat gaggagctat ttttatttgg ctattcttat tacttgatgc
     8821 agaacagctt ttcacaacct cttttcgtct attttttatt ttcgagattt ctctttcctt
     8881 taaaaacaac tgtttgaaag aagaacgcac atccttgaaa aacaagcccg ctaaccgtta
     8941 cacctaacca gccatactgc tgccacattg tcgtccctag caatgaacca gccgcacctc
     9001 caataaagta cataaacata aataca
//
LOCUS       DAZUMK010000107         8790 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000107 DAZUMK010000000
VERSION     DAZUMK010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.109"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            105..752
                     /locus_tag="ACG808_002286"
     CDS             105..752
                     /locus_tag="ACG808_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047886.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(845..3124)
                     /gene="helD"
                     /locus_tag="ACG808_002287"
     CDS             complement(845..3124)
                     /gene="helD"
                     /locus_tag="ACG808_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFS5047887.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            3402..5225
                     /locus_tag="ACG808_002288"
     CDS             3402..5225
                     /locus_tag="ACG808_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS5047888.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5272..5451
                     /locus_tag="ACG808_002289"
     CDS             5272..5451
                     /locus_tag="ACG808_002289"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFS5047889.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            5788..7728
                     /gene="thrS"
                     /locus_tag="ACG808_002290"
     CDS             5788..7728
                     /gene="thrS"
                     /locus_tag="ACG808_002290"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFS5047890.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            7952..>8790
                     /locus_tag="ACG808_002291"
     CDS             7952..>8790
                     /locus_tag="ACG808_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS5047891.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFM"
BASE COUNT         2764 a         1567 c         1876 g         2583 t
ORIGIN      
        1 ttttgtgtga tttattttct tttctaattc aacctgtggt atgatgagag cataagtatc
       61 atgtgaaatg atatgaagta tgtggaagat ggacggggaa gaatatggaa gcaaaaatca
      121 ctttagaacc ttttgaacgt atcttaagcg gttaccgcaa agtagaagag ttagctgtga
      181 acgtaactga ttgttcaaaa ttagcgcaaa aatacgcaag attcggcgtt gaaggctatc
      241 gattaggaaa ctatgtcggt acagggtatc tgaaccgtta tttagagtgt atggtggacc
      301 gcgcaccaat gctgatttat agacaaaatt atctgattcc gctgcttttt cgtcgttctg
      361 attcagcctt tcggttattt gaagaagagt atcggatgga ggctttcttt ctactattag
      421 agtggagctt gaaacatcgt cctgaaaaaa ttcttatcga aagaaatgag aagatagaca
      481 cgaaaaaaaa taaagtgatc gatagtgcat acctagcatt tcgtgtatca gagattttgg
      541 attgtggcgg ctatccaatc agcaatttcc aatcaattga ccaattcatt gaatggaatc
      601 gaatttaccg attgatcgac aatggcggga ttggccgtca tagcaaggta tttgacccag
      661 aatatccaga aaacatggaa gaactgaaaa tgattatttc gcttgtcaaa ttaaaatatc
      721 ctgaaacaga tttggattta tatatagaat aaatgacctt aaatgattga attacttttt
      781 ctgcaattat tattgtaaat aaaaagacaa ctgctaacat taatgatagc agttgtcttt
      841 ttacttacag tacttttttc tcataagatg aaatatccat attgtttaac aaaggacttt
      901 cattattgga ggtaaaaagg gtcaattcat gcatcgcacg tgtacacatg gtgtaaagga
      961 tcaatttgtc ttgctgagaa gaaaaatgtt ctgcatctac atcccacaag aaaactcggt
     1021 caaactccag accttttgct aaaaaggctg gaagtaaaat aatggaacgc ttcataaagt
     1081 cactctcatt taaaatcagc tggacttgtt gtttcaactc gtctggcagt tgagcataaa
     1141 gagcttcgca ttcttcttgt gttttagcta tgattgctgt acgccaatac cgggcttttc
     1201 cctgagtcgt tcgaatggta tctgctaacc aattcaattt gatatcatct gttgttcctt
     1261 ggatgagcaa aggaaggtct ccttttcttg ctgttgtttc tacttgatgt tctttcacaa
     1321 taaaatgatt agcaaaatca gtgatttctt ttgtggagcg atagcttgtc gttaattgga
     1381 aacgagtgac ttcacggtcc acgaaaaggt cttccaatga tccaacgatc gtttcatttc
     1441 caaagacatt ttgatttaga tctccacaga aagtcaaatt tgcttttggg aacaaggctt
     1501 gcagcaatgc cacttgtgat ggtgcgaaat cctgcatttc atcaataaag atatagcgag
     1561 ctttgtgtgt aaccgtcact ggatatgttc ggcgcatcag taaaaagaat agtacagcat
     1621 cctcttgacg caattctctt tgacgcaagt gtgtacgaac agttgtcaaa tgttcctgcc
     1681 acctggtctc gctgatttta tgtcgtgtta gaagagaaag tggcacagtt tgtagaaagt
     1741 ggagatattg tttcgttata ttgacaaatt gataattcat gatccctcga tggattcgac
     1801 ggaatctttt acgtacgatt tggttggcca gttttttacg taatgaacgc tccttttgtt
     1861 ctgtatattc aagatttgga tctgttgcgt atagttcttg gagctgttct tccgccagat
     1921 ctttgaccca ttgctgacgt gtttcgtctt tttgcagacc gcctaatttt ttcaggagct
     1981 tcgtttgtaa tagagaaagt ctatgatgta aaggtaattg ttcatttgtt tctttatacc
     2041 attggcgaat cgattccttt gataggatcg tacggccatt tattttcata tcacggaaca
     2101 agggaccgta gtttgtgatg ccttggatat agcgatcaat ctgatcgact aaggtcaatc
     2161 cgcttttcat cacttgtatc ggatcttttt cccctagcaa aaaaccactt tcttgttgct
     2221 gttcatctag taaagaaaac tctggcagca gctgcaccaa ataatttttg aatgtctgtg
     2281 taggcacgcc gctttcacct aatgaaggca atacagtcga gatgtaatca gaaaacaaat
     2341 ggttgggtga aaatagaaga acgttttcag cctcgagcca cttgcgatta tgatacaaca
     2401 gaaaagcaat acgttgaagc aaagcggatg tctttccact tccggcgatt ccttcaatca
     2461 gcattacttt acttgttgtg tcccgaatga ttgcattttg tgctttttgt atcgtagaga
     2521 cgatgttttt catatggttg cttgatgatt catccaatat ctctagcaaa aagtcatcat
     2581 tgattacttc cgacgtatct accatagata aaatacgtcc ttcttggatt ttaaattgtc
     2641 gtttcaactg cagatcgaca tctattctat ctgtttcagt ttcataaaat gcgggaccaa
     2701 tctcaccttc ataataaaga ttggcaattg gtgcgcgcca gtcaataacg attgtttctt
     2761 ctgccagatc tcttaaagaa gcgataccta gatataatgt ttctgtttca cggtcttctt
     2821 taaagtcgat acgggcaaaa taaggactat ctttcatgac agacaacgtt tgcaggcgtt
     2881 tttgctgtgc ttcaagagta tggtatttca aaagcaattc ctgctcatgt tggcgatatt
     2941 caacagcaga ttcataaaaa gcttcattgg aaccagctcg aatcttgtga ttagctgtgt
     3001 cttttgtcga atgattcatt tcctgagtca attcttgttg ttttgtatcc agcatatttt
     3061 gttcgatctg gattagttcg atcgtttcat ccacgtgcgt ttgttctagt tgtttctcat
     3121 tcatcgcatg tttgctcctt ccgccgtaaa taacgaatta aaagtatagc atagtttttc
     3181 gaaaaaaggt actttttaac tgatgaatcg aaaaagtaat ccttgctgaa tatttttgtt
     3241 tgtagaactg atgaattttg gaaacgctga tagtgatagt tgttttcaat taaggcctat
     3301 tgttttaaaa agtgctagtc aaggactatg cttaagatga aagaggcagc tggaaattta
     3361 ttactaacct ctctaagtca cataaaagag gagaaaaaac aatgacacat ttcaaaaaat
     3421 ttttacttac tttgcttatt ggtgggatag ctttattaag tgaatttgtt tttcaccagc
     3481 cggcccttgc atttgcgatt gtcgcattta ctggagggat tctcgccttc ttgatgtttt
     3541 tggaaatgat caagacgtta cgttcaggaa gatatggcgt agatgtatta gcgattaccg
     3601 caatccttgc aacattagcc gtcaaagaat actgggctag tttgatgata ttgatcatgc
     3661 ttacgggcgg agatagtcta gaggattacg cgaataaacg agcgggtaaa gaactgcagt
     3721 ctttgttaga taacacccca agaattgccc atcgattcaa agaaggaaaa ttgcaggata
     3781 tagtaatcga tgaaataaat attgatgacc agttactagt caagcctggc gaacttgtac
     3841 ccgcagatgg tcgagtgacc aaaggagaat cttcggtcga tgaatcttct ttgacaggtg
     3901 agtcccgccc ggaaaccaag aaacctggag atcagattat gtcagggtct ttaaatggag
     3961 acggatcgat ccaaatgatc gctgaaaaac gtgccgcaga cagccagtat caaatgatta
     4021 ttaaactagt cgaagaatca aaagaaaagc ctgcccgatt tgtccgttta gctgatcgtt
     4081 atgcagtccc tttcacgttg attgcttatt tgatcggtgg tatttcttgg tggttctcaa
     4141 aagacccttt acgatttgct gaggtactgg tagtggcatc accttgtcca ctgattctag
     4201 ctgcgcccat tgctttagtt gcaggaatga gccgttcaag taaaaacggg atcgttgtaa
     4261 aaactggaac aacgattgaa aagctcgcac gtacaaaaac gattgctttt gataaaacag
     4321 gaacgcttac caaagggata ttggaagtag aggcgattca gccagtcaat ggaataaaaa
     4381 aagaagagct acttctgtat gcagctagtg cagaacagga atctagccat atcttagctc
     4441 gttcgttagt gaacaacgtg aaaaacagcc aactgtatcc tgttagtcaa ctgaaagaag
     4501 tgaccggtca aggaatccaa gcagtagtca ataatcatga agtaaagatt ggacgtgcct
     4561 cttttgtttc agctgaagaa atagaaacgt ccaatacagc aatctatgta acaattgatc
     4621 gatcttttgc aggaacgatc ctattttcgg atcagattcg cccagaagca gcaaagacaa
     4681 tcaaggagct aagaaaactt gatgtgaaaa atttgatgat gatcaccggg gatggatctg
     4741 ccattgctca aacgattgca gaaaaagtcc aattagattc tgtacatgcc cgtagcttgc
     4801 ctcaagataa actgaccatt ttagaaagta tccctaaaga tcaaagacct gttgcgatgg
     4861 taggagacgg cgtgaatgac gcaccagcac ttgctgctgc agatatcggt attgcaatgg
     4921 gggctcatgg ttctacagct gctagtgaaa gtgcagatgc ggtcatcttg aaagatgatc
     4981 tcttgcgtgt agcaaaagca gtagtgattt caagagatac gatgagaata gcgaaacagt
     5041 ctgtactgat tggcatcttt atttgtattt ttttgatgtt aattgcaagt acgggactaa
     5101 tccccgcatt attcggagct gttttgcaag aagtcgtgga tacggtctct attttgagtg
     5161 ccttgagagc aaaaaaagat ccagccattt ttcctgattc agtacccaag acaaacatag
     5221 tatgatagaa tcgagttgaa aaaagaacga tcagaaagag gagagagcat tatgccattc
     5281 gtacatgtcg aattagtaga aggtcgcagt ccggaacaat tagaaaatat gatgaaagat
     5341 atcacggaag ctgttcataa aaacacgcag gcaccaaaag aacacatcca tgtcatcatc
     5401 aatgagatga aaaaaggaac atacggtgta aatggggaat ggaaaaaata agtttagttt
     5461 cccacttgat ttttctattt attagtggtt aaataaacac ataattgatt gatgagaaag
     5521 ccaactgaat ttgagagtct tcgataagag aggaaaagct aggctgaaac ttttccagga
     5581 gaaaaccaaa ggatgaccac tttcgaaacc cttgttgaag caagggcggt cggaccgtta
     5641 caacgcttga ggaacgcatt tgcgtttgaa cattaggtgg aaccacgaac ttttcgtcct
     5701 agtatctctt ttcaagagat gctaggattt ttttgttttt cagcttgatg atctgatcaa
     5761 tcaaaacatt tcaaggagga aactattatg ataaaaatta cttttccaga tggtgcagtc
     5821 aaagaatttg agtctggaac aacaacattg gcaattgctg aaagcatttc aaaaagctta
     5881 gcaaaaaaag ctttagctgg taaagtgaac ggaaaactcg ttgatttgac tcgtccaatc
     5941 gaggaagatg caagtattga gatcatcaca ccagaccatg aagatgcact tggcttagtc
     6001 cgtcattcag cagctcattt gatggcccaa gccatgcgtc gcctctatcc aaatatccac
     6061 tttggcgttg gaccagctat cgattcaggg ttttactacg atacagataa tggacaaaac
     6121 caagtaactg cagaagattt gccggcaatc gaagcagaaa tgatgaaaat cgtcaaagaa
     6181 aatctgccga tcgaacgccg tgttctttca aaacaggaag cattagaaat ttttgcaagt
     6241 gatccgtaca aagtagaact gattagtgaa ttgccagaag aagaagtgat tactgcttat
     6301 caacaaggcg aattcatcga tttgtgtcgt ggaccacatg ttccatccac tggccgtata
     6361 caagtattta aattattatc agtggcaggt gcttattggc gtggaaactc aaacaatcaa
     6421 atgatgcaac gtgtatacgg gacagccttt tttgacaaga aagctttgaa agaatatatc
     6481 cgtctccgtg aagaagcaaa agaaagagac catcgtaaac taggaaaaga acttgacttg
     6541 ttcatggttt ccccagaagt aggttctggt ttgccattct ggcttccaaa aggggcaaca
     6601 atccgacgca cgatcgaacg ttatatcgta gacaaagaag ttagtctagg ttaccaacat
     6661 gtatatacac caataatggg agatgttgag ttgtacaaaa catctggaca ctgggatcat
     6721 tatcaagaag acatgttccc gccaatggat atgggagatg gcgaaatgct tgttcttcgc
     6781 ccaatgaact gccctcatca catgatggtc tacaaaaaca cgatccattc ttatcgtgaa
     6841 ttaccaattc gtatcgctga attaggaatg atgcatcgct atgaaaaatc aggtgctttg
     6901 tcaggtttac aacgtgtacg agaaatgacg ttgaatgatg gtcatacatt tgttcgtcct
     6961 gatcaaatca aagatgaatt taaacgtacg cttgaattga tggtggcagt atatgctgac
     7021 ttcaatatta cagattatcg tttccgcttg agctatcgcg atccaaacaa tacagataaa
     7081 tattttgatg atgacgcaat gtgggaaaaa gcccaagcaa tgctgaaagc cgctatggat
     7141 gaattagaac tagattactt tgaagcagaa ggcgaggcag ccttttacgg accaaaacta
     7201 gatgttcaag tcaaaacagc tcttggtaca gaagaaacat tatcaactat acaattagac
     7261 ttcttattgc cagagcgctt tgatttgaca tatgttggag aagacgggga aaatacgcat
     7321 cgtccagtag ttatccaccg cggaattgtt tctacaatgg aacgttttgt cgcttattta
     7381 acagaagtat acaaaggcgc ttttccaaca tggttggcac ctatacaagc aacaatcatc
     7441 cctgtttctg ttgatgcaca tggcgattat gcttatgaaa tcaaagaacg tttgcaaatg
     7501 aaggggttgc gtgtagaagt tgatgatcgt aatgaaaaaa tgggctataa gatacgtgcc
     7561 tctcaaacac aaaagatccc ttaccaatta gttgttggag acaaggaatt ggaagatgca
     7621 acagtgaacg tacgtcgtta tggaagtaaa gaaacagctg tagaagattt gaatatcttt
     7681 attgatgcta tggaagcaga agtcaaaaat tacagcagag aaaactaata ggtaaatttc
     7741 ctgtaaaaaa gtagtagata gaaagcaatc cgaacaatag ataacaggta ttcctcaaag
     7801 acacagatgc tcaatagaat ctgtgggatt gatgggataa gatattctgt tgggcggatt
     7861 gtttttttgt tttttatcca gtatacgacc gtgaataagg gtctgatatg gtatacttac
     7921 atagactatc attaaaaggg tggaggaact tatgagcgta attagtcgta ttgaaagtat
     7981 gatggaagat tattcttcag cagaaaaaaa gttggccaac tacattatcg gtaatattga
     8041 gaaggtccct acaatgacag ctaatgaatt agcagaagct tccgggttga gtgcgccgac
     8101 agttgtacgt ttttccaaaa aaatcggttt tcaaagtctg acggacttca aaatcacgat
     8161 ttcaacagaa ctacagactg gaatcgatga aggttttagt gatatagaag caaatgagtc
     8221 cttttactct atcaaaaaca aacttggtaa caatgcacaa gtagcgataa aagaaacagt
     8281 agatattcta gaagaagaaa cgatccaaaa agtcgtagag tgtttggaat cggcagagac
     8341 tgttttttta tatggggttg gcgcctcttc tttagtagta gaagatattc tgcaaaaatg
     8401 gtctcgtgtc ggtaagccaa tcatttttga aaaagatatc cacgtcttgc tcccgcaact
     8461 tgtcagcaat gagaaaaaga aagtgctttg gcttgtttca aactcaggta gaagtgcaga
     8521 tgtagtcgca ttagccgagc tggcgaaaag catgaatatc gaaatcattg cgttgacgca
     8581 atttggaaat aacccgctta gcaagatagc cgatgtactc gtacaaacat ctagaccaaa
     8641 agaaatcacg aatcgaagtg cggcaactaa ttcacttttg gcccaatttg ctacgatcga
     8701 tatcattttt tatttctata tggctaaaaa cgaaaagctt agcgaaaaag tgaaaaaaac
     8761 acgagaagca atccaagaat attttatgga
//
LOCUS       DAZUMK010000108         8676 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000108 DAZUMK010000000
VERSION     DAZUMK010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.110"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            75..977
                     /gene="gnd"
                     /locus_tag="ACG808_002292"
     CDS             75..977
                     /gene="gnd"
                     /locus_tag="ACG808_002292"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFS5047892.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            979..1980
                     /locus_tag="ACG808_002293"
     CDS             979..1980
                     /locus_tag="ACG808_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFS5047893.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            1977..2261
                     /locus_tag="ACG808_002294"
     CDS             1977..2261
                     /locus_tag="ACG808_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFS5047894.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            2294..3091
                     /locus_tag="ACG808_002295"
     CDS             2294..3091
                     /locus_tag="ACG808_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFS5047895.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            3105..3938
                     /locus_tag="ACG808_002296"
     CDS             3105..3938
                     /locus_tag="ACG808_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS5047896.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            3956..4813
                     /locus_tag="ACG808_002297"
     CDS             3956..4813
                     /locus_tag="ACG808_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS5047897.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            4835..5344
                     /locus_tag="ACG808_002298"
     CDS             4835..5344
                     /locus_tag="ACG808_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFS5047898.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            5381..5782
                     /locus_tag="ACG808_002299"
     CDS             5381..5782
                     /locus_tag="ACG808_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047899.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            5894..6247
                     /locus_tag="ACG808_002300"
     CDS             5894..6247
                     /locus_tag="ACG808_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047900.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            6281..6724
                     /locus_tag="ACG808_002301"
     CDS             6281..6724
                     /locus_tag="ACG808_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5047901.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            6741..7826
                     /gene="uxuA"
                     /locus_tag="ACG808_002302"
     CDS             6741..7826
                     /gene="uxuA"
                     /locus_tag="ACG808_002302"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFS5047902.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            7819..8532
                     /locus_tag="ACG808_002303"
     CDS             7819..8532
                     /locus_tag="ACG808_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5047903.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         2906 a         1360 c         1966 g         2444 t
ORIGIN      
        1 aaaatatttt tgtaaaatct atttacaaaa aaattttttt gtataatgag ggtataagaa
       61 atggaggcgc tatcatggaa attggtgtaa ttggtttggg aaaaatggga ttgaatttag
      121 ctttaaactt aaaagagcac catcacaaag tacaaggact cgacatttca agtacagctg
      181 ttagtcaggc aagagaaaat gacattcctg cttatcaaga gatctctgaa tttctagcta
      241 cttttgagca gaaaaaaatc atctggttaa tgcttcctgc tggagagata accgaaaaca
      301 tgttaagaaa actttttcca aaactcggac caggagacat tattattgaa ggcggaaact
      361 cttattataa agattcaatc cgtcgagcag aagagtttca gaaaaatgaa atcggttact
      421 tggattgtgg gacttctgga ggaatcgaag gagcacgaca tcaagcctgc ttaatgattg
      481 gaggagataa agaaactttt ctatccgtgg aacaattatt caaagatgta gcaattgaaa
      541 gaggatatct gtatgctggt gcatccggaa gcggacactt cttgaaaatg atccataatg
      601 gaatcgaata tggaatgatg caagcaatcg gtgaaggatt ccaacttgta caaaaaagtg
      661 agtatgcttt tgacttggaa caagtcgcac gtgtttggaa tcatggttcg gtgattcgcg
      721 gctggctgat ggaaatcatg gaagatcagt taaacgaaca tcccaattta agtaattatc
      781 ggggaatcgt ttctgcatca ggtgaagcaa aatggacggt tgaaacagcg ctagaaatgg
      841 ctgttccagt tccagtaatt gcaactagtt tgttcatgag gaatctttca caggaagaag
      901 atagtttttc agcaaaagta gtttcagcac taagaaatgg gtttggcgga catgaaatag
      961 ttgaaaaggg tgagtaaaat gccagattat catatagcaa tcgttaattc cagcagtttc
     1021 ggaagagtct ttccacacca cttggaaaga ttaaagtcag ttggagacgt tcatttcttt
     1081 actgttgatc aagagattcc aggaaaagaa ctagcggagt tacttaaagg attcaatatc
     1141 attattgcaa gtgttacgcc attttttcga cgagattttt ttgaaaacaa agatgaactg
     1201 ctattgattt cccgacatgg tattggttac aacaatattg atttaaaagc agcaaaagaa
     1261 caccagacaa tcgtatctat catacctgct ttagttgaac gaaatgctgt agcagaaaat
     1321 aatataacta atttgttgtc catgcttcga cgcacgacac aagcacaagc aagagtcaag
     1381 gacgatcgat gggaagatcg ggcggagttc gtcggtagga cgctgtttaa caaaacagtt
     1441 ggtgtgattg gtgcaggaaa taccggaagc ggagtaatcg aaattttgcg taacggtttt
     1501 cgctgtgatg ttctagctta tgatccttac aaatcagatt tatacatcga aagctttggt
     1561 gcaaaaaaag tagaacttca agaattactg gaacaatcag atatcatttg tttatgtgcg
     1621 aatttgacag aagagaacta tcacatgatt tctgataaag aagtcgatca gatgaaagaa
     1681 aatgtttatc tatctaatag tgcaagaggc gctctagttg atgaagaagc gatgattaat
     1741 ggtctgaaaa gcggaaagat cgcaggatat gccacagatg ttttagaaga agaaccggga
     1801 cgtcaagatc atccgtattt agcgtttgaa caagtagtga ttacgccaca tatatcggcc
     1861 tacacaaggg aatgtttaga agcaatgggt gaaaaatgtg tcagagatgt cgaacagatc
     1921 agtcagcacc ttcttcctga acgttctgtc cagacaaaga gcaactatat aaaaggatga
     1981 tgaagaaaat ggtgaacggt ttaaaagtga aaacaggccc tcaattttat ttatatgaag
     2041 aaggtggtat ttcaaaagtt tctgatctct tgaaatcata cggcgcgaaa agagtactgg
     2101 tcacccacgg aacggtatca tgggaaaagg cgctacctaa attagtattt ttgaatgacg
     2161 aaacgattca atttttttat catcgttatt ctggagaatg tagttatgct gaagccagac
     2221 gaatagctac aatcatcaaa aaaatgaaat cgatttcgta gttggcgttg gcggaggaaa
     2281 actgaccgat ctagtgggtt atagtgccaa tcttgcaaga atcgatttcg gagtcatccc
     2341 tactttagct agtaattgtg ctccatggac acctcttgct gtcatgtata aggagaatgg
     2401 acaagcagaa gggaaaacag aacactttta cagacaagct gcttttttta taacagatcc
     2461 ccaattagtg attgattccc ctgttccata tttcattgcc gggttagcag atacactagc
     2521 aaaatggtat gaatctgagc tcatcttaga acaagatttt ttgcaagatg aatcattttt
     2581 gaagctagca caagatactg cgaagatctg caaagaagag attttgaatc attctgctaa
     2641 agcaatccaa gatatggcac aaagaaaact gacatcggag ttcaaacagc tttcagagat
     2701 tatctttgct gttgctggtt tagttggagg attaggagat aaatacgcaa gaaatgtggc
     2761 agcacatgct atgcatgacg ctatcagtaa atatattccg gaaagccatc gttttctaca
     2821 tggagaaaaa gtagcgtatg ggattttcta tcaactggca ctagaaggtc gctgggaaaa
     2881 aatagacgag ttatatcctt tgtatcggga attagaactg ccggtttctc tagaacaaat
     2941 gggattattt ccgaaagata aacagataat agataaaatg gctgaattta ttgattcgaa
     3001 agagaaagtg catttgattc ctatagaggt aacacagaaa tcactgatac agtcaatgat
     3061 ggaactggaa ggttatatca gaaagatctg aaggaggagg acacatggaa acattaagtg
     3121 tagtacaaag tttgttgatt gctttatggg tagccgcggt tatgtcccgc tggttaggtg
     3181 gaggagccac gttgacctta cgtttttctc ctttgatgac tggattagtc gtcgggttga
     3241 tcatggggga agttgcacaa gcgatgattg ttacagccgc tttgcaaatg atttatatgg
     3301 gggttttttc tccaggagga tcaatgccag ctgaaccatc tattgcagca gctatcgcag
     3361 taccagtcgc attattaggg gatctgtcgc cagaagcagc gattgccgtt gcggtgccag
     3421 tcggattgct cggaagttac ttgtatcagt ttcgtttttt catcaatact tttttaggta
     3481 aatatacaga caaagctgta gaagatttga atacaaaagc tatggcccga tcaattattc
     3541 tttatccaac ggttgcctca tttttattat tcgttccgct tgttttcatt gctttatatt
     3601 taggggcgcc attgatcgct gatgtaatca aagctttaga gggaacagtc gttatccaca
     3661 ttcttgaagt agtaggtgga gggttagcgg caatcggtat cgcgacaact gtatacgtta
     3721 tcggtcgaaa agattatctg gtctttttct tccttgctta ttttatgagt gttgttttga
     3781 aatctttaga tattacgatg gttacttatg ccatttttgg gattattatc gctttgatct
     3841 ttgtccagtc gcaaaaaggg aaacaagtac agcctgccac aagcaataac aattcagcat
     3901 cttttgatga cgaagatgac tatgatgacg gattttaatt taggaggaat gggaaatggc
     3961 tgaaataatg gataatcaaa caggtgctcc tgaagaaatc acgaaaaaag atgtaacaaa
     4021 agcctatctg agatggcatt ttgcaaacga aattcctcac tcttttgaac gttacttggc
     4081 gccatcacta ctatacggaa tgatgccaat cttgaaaaaa ctttataaag atgaagactc
     4141 tttaaaagca gcatataaac ggcagttact gttcttcaac actcaattaa gctggggtgg
     4201 cggtgtgatt actggcctaa tgtcttctat ggaacagcaa agagcagaag aagagtatga
     4261 agaaaaagag atcttgatgc aggatgactt gatgtataac acgaaagcgg gattgatggg
     4321 ggcactagca ggaatcggag atgcaatcga ttcaggaacc gtccaatata tttttatcgc
     4381 aatcgcggta ccttgggcac aacagggaag tcctttaggc gcactttttc cttttatctg
     4441 ctttgggttg tatcaggttt tactaggggt atttttcgca agacaagctt tcactatggg
     4501 ccgtaatgct acaggcctaa agcaaaacac tggtgttcag acagcgattg aaatgctatc
     4561 agttttaggg ttgtttatga tgggggtgct tgcgggaaac tatgtaaaag tagaatcttc
     4621 attgaaattt gcgatatccg gacgtgagtt tgttatccag gaaattcttg atcaaatcgt
     4681 gccagggata ttacctttag ctgtcgtaat gggtgtgtac ttgttctata cgaaaaaagg
     4741 attgaaagtc acacaagcat tattatggct gactggaata ttaattgtgc tagcaggaat
     4801 cggtatttta taaaaagaag ggaacgtgaa taagatgggt gtagtcaatt tagcgcgtgt
     4861 ggatgagcgt ttgatccatg ggcaagtaat ggtaacgtta tctcaaaaaa gtggagtgaa
     4921 ttccattttt gttgtagacg aagttgtagc aaaagataaa tttatgagag atctttacaa
     4981 aaatgcagga agccgtacag gacaaaaaac aattgtcatt acaccagaga aagctaaatt
     5041 ctattgggat gaatatcaat ttaaagaata caattgtatt ttgatcgcaa aaacagtcca
     5101 aacgatttac gacttagtaa aacacggaat ccccatggaa gaattgaata ttggagggat
     5161 tgctcaaaaa aatcctgata cagatctttt agtgacgaaa tctgtttatt taaataaatc
     5221 agatgctgaa aaattgaaag aaatgaatga acaatatggc gtaaagaata tttattttca
     5281 agccactcca tctgcaccta aaacaggatt aaaagatgta ttgaacaagt ttgatttgtc
     5341 ataaacaaat gcttagaaaa aagataaaga gggcgaaaaa atgaagaaga acgagctgtt
     5401 tcgtgattgg gaatttcgtt atcgctatat ttaccgtaag cgccgtacaa aaaagagtaa
     5461 acagcgtttt ctttcagcat tagtatcaga tatttacagt atgagaacag atgtgacagt
     5521 cattgcttac gatacgctag cctaccgatc aaaaaatatt tatgttggag atatcgaaaa
     5581 agcagagaaa gttatctgta cttattacga tacacccgtt catgcattag gaagttattt
     5641 catgtttgat tggaaagacc aaaggaaaaa aacaatctac tctatattat tgtccttcat
     5701 tttgttgttc agtttaggtt ggtggggaat gatgatatat aacaaaaatc ctcatcacgt
     5761 attcgatctg ctatccgtat aaacaggtat cactgtacta gcatttggga gctacttttt
     5821 cttattggga aaagcagctc gcggatggcc tagcagacaa acttttattc gaaatacttc
     5881 atccatatta acgatgctag aaatgatccg tacgattgat gatccgagtg tagcgtatgc
     5941 gtttgttgat gaaggctgtt atggagaaaa gggcttagac agcgtgagat cgggtatgaa
     6001 aaaagaggct attctttttt atctagactc tgttggggca gatacaccgc tacagttttc
     6061 gggaaattat ttttcaaata aagaacagtg gctgaaacaa gttgataagt tgaaagaaaa
     6121 aaacgtcaat tatattttta gtgcaagaaa aaagcaagcg cagttcttct atttgaccaa
     6181 aacagattta cgagggaaaa catttaattg gcaaaatgcc aatcagatta ttgctctttt
     6241 tcgataagta aaaagtgcaa aacaaggagt gagaagacta atgttggaaa tcatgatcgt
     6301 tacacatggt aagttgagcg aaggactgaa agatgcggcg gaagtaattg tcgggacatc
     6361 gagccatatt gaaacaatgt gcttagagtc tggggaggac atccaaacat tcggagagaa
     6421 ggtcaaagaa aaattaacta tgatcaacac agcagaaccc atgctagtat ttgttgatct
     6481 aattagcgca agtccgtaca atcaagcgct cttggcagtc aatgaactga ctgaagaaaa
     6541 gaaagcagcc acttatgtca tagggggcgt gaatctgcca atgttgttag aagcaatcaa
     6601 tcatcagatg cttcacacac cgatagaaga agcggtggaa caaattgtca aacaaggaaa
     6661 agataattta actgtttggc atgcttcagc ggaagaaaaa aaattagatg aggacgattt
     6721 ttagaaggaa ggctgaaaag atgaagtggg gatttagatg gtatggtgaa aaaggggatt
     6781 cgatcccatt aaaatatgtt cgccaaatac cgggaataaa tgggataatt ggaactttgc
     6841 tgactaaaat gcctggagat gtttgggaga tttcagaaat tgaggagttg aaagcatctg
     6901 ttgagagcca aggactggaa ctgcttggaa ttgaaagtgt agcgatccac gatgcaatca
     6961 aggcagggac agatgaacga gattactata ttgataacta tatacgaaca atcaaaaact
     7021 tgtctcgatg tggggttcga ttgatttgtt atagcttcaa accaattttt ggatgggcga
     7081 aaactgattt gtttaatgaa aacaacgatg gaagctattc tcttttatac gatcagaaaa
     7141 tcgtcgatca gatggagcca ggagaaatgt atcaattgat acacagccag tcaaagggat
     7201 tcaaattgcc gggttgggaa gaagaacggt tgaaaaaatt cgctcagctg gaaaaactgt
     7261 atgaaggaat gaccgaagaa gaattatttg aaaatctgac ttatttttta gaaaaagtga
     7321 ttccagtgtg cgaaaaagca gatgtaaaaa tggctatcca tccagatgat ccgccatggg
     7381 aactttttgg ttttcctaga gtgacgaaaa atcttggcga cttgaagaaa attctaaatg
     7441 cagtagattc tccatataat ggactaacgc tgtgcacagg atctttaggg gcaaatccgg
     7501 aaaacgattt agttgaaatc atccatgagg taggttcgcg catccatttt gtccacttca
     7561 gaaacgtgga gtttttagga gaaagaaagt tcaaagaaac ggcacatccc tctacagaag
     7621 gctcccttga tatgtatcat attatgaaag cgttggtgca agaagggttc gacggtccta
     7681 tacgccctga tcatggtaga accgtgtggg gtgaagtggc tatgcctgga tatggcctgt
     7741 atgatcgtgc aatgggactc acttacatgc aaggtctata tgaggcgata agtaaggaaa
     7801 aaccactggg agagcattat gaataaaatc aagctcttca tctttgatat ggatggcctt
     7861 ttattagaaa ctggacgttt agcttatcgt gcttatgtaa aatcagcaca aaagtatgat
     7921 tatgaaatgc gtaaggaagt ctattattta ctaactggcc aaacagaaat ggcgataaga
     7981 gaacaaatgg gcattttgta cggagaagat gttccatata tcaaatggcg agaagcaatc
     8041 aatcaatata aagaaaaaat cgtgaaagaa gataagagag tatatacgaa aaaaggagca
     8101 gaagaaattt tatcttttgc aaaagaaaga ggaatccata caatcgtggc ttcgtctaat
     8161 acacgggtaa aagtagaaat gtatttaaaa atggaaaatc tatatgcctt gtttgatcaa
     8221 atcatttcag gggatgatgt aaaaaaagga aagccggaac cagagatctt tttgaaagct
     8281 tgttcaaaaa tgaatattcc tccaagtgaa gcacttgtat ttgaagactc gattgcaggg
     8341 atcgaagcag caaggcgagc gggcattttg tcttttctca tcaaagatca tttgataggg
     8401 ttgcccgatc ataatgggaa gcataagttg aaagtagatg taagtttata tgaatccaat
     8461 aaagcagact atgaatttta tgacctgcat caagcaaaac gatttttaga aagtaaaggg
     8521 ttgtatctct aataaagagg actattttct agtcctcttt atttattgtc ctgcacgact
     8581 gagggaacag atgaaaaaga agtaattttt tattttgaag tatctacctt atttgtagtg
     8641 aacaatttag aacaaacaca aaacgcatct actgtt
//
LOCUS       DAZUMK010000109         8576 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000109 DAZUMK010000000
VERSION     DAZUMK010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.111"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            150..929
                     /locus_tag="ACG808_002304"
     CDS             150..929
                     /locus_tag="ACG808_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047904.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQHHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSLIEQIRNEKIRAAYASKAETVAQQMGYSNTSY"
     gene            970..1914
                     /locus_tag="ACG808_002305"
     CDS             970..1914
                     /locus_tag="ACG808_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFS5047905.1"
                     /translation="MAKRRRRSRGKKIFLIILAILVVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGHDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYRDAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            1930..2709
                     /locus_tag="ACG808_002306"
     CDS             1930..2709
                     /locus_tag="ACG808_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFS5047906.1"
                     /translation="MEETISLKEIFETLRKHVITILISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQATSTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAPTQPKATNDKTAPSRRSRSRV"
     gene            2722..3420
                     /locus_tag="ACG808_002307"
     CDS             2722..3420
                     /locus_tag="ACG808_002307"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFS5047907.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENMSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            3448..4212
                     /locus_tag="ACG808_002308"
     CDS             3448..4212
                     /locus_tag="ACG808_002308"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFS5047908.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIDRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEQLFFELLQLGKTPVIVHPERNAHFRKDPNHMLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYKQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEIKKKKFGLF"
     gene            4225..6042
                     /locus_tag="ACG808_002309"
     CDS             4225..6042
                     /locus_tag="ACG808_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFS5047909.1"
                     /translation="MFVLTRRRKIATLVVVDSLLLIAANIAAAKFMKPFVAIPMDLIL
                     ISTGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFGSLSASDVSSILILLFINRQYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYVSADSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIQVVGFVDDDPNKRNTYLSGKKVLGALKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESAHVKVNTMPSIEELASGKISVSKLKTIDVVDLLGRDEVKLDIESI
                     KDQITDKVILVTGAGGSIGSEICRQIIQFNPAKLLLLGHGENSIYLIDRELRTHHQNC
                     PTEIVPIIADIQDREKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAKGGPITVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSIQKVMDFVKSLPQDDEQLAKDIVTFANASNK"
     gene            6056..6205
                     /locus_tag="ACG808_002310"
     CDS             6056..6205
                     /locus_tag="ACG808_002310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HFS5047910.1"
                     /translation="MNKLEKRQIPFSPPDITDLEIKNVVEVLKSGWITTGPKTKKLEK
                     KLQSG"
     gene            6148..7278
                     /locus_tag="ACG808_002311"
     CDS             6148..7278
                     /locus_tag="ACG808_002311"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HFS5047911.1"
                     /translation="MDNYGTKDKKIRKKITKWLGTSKVVCLNSQTAAAETGLRLLGIG
                     ENDEVIVPAYTYSASASVVCHIGANLVLIDCQQDSLEMDYDALEAAITEKTKVIIPVD
                     LGGIPCDYDRIFEIVEKKRNLFQPSNEIQKAYGRIIVMADSAHAFGANWHDQMVGNVA
                     DLTTFSFHAVKNFTTAEGGALVWKEHDKLDNEAMYHQCQLLSLHGQSKDALTKTQLGA
                     WEYDIVGPWYKCNMTDVTASIGLAQFERYPNILKRRKNIIKRYDEAFQQLGIEVLMHY
                     TDDYSSSGHLYITRVPNISSEQRNEIIIKMAEQGIACNVHYKPLPMFTAYKNMGFDIN
                     NYPNAYAHFVNEITLPLHTKLTDEEVEYVINNYSKIVKDLVG"
     gene            7292..8026
                     /locus_tag="ACG808_002312"
     CDS             7292..8026
                     /locus_tag="ACG808_002312"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003508523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFS5047912.1"
                     /translation="MRKWEDLPKEMQNDEIRYYYDILSKKKFSLVVKRVFDILVSATL
                     LLTLSWLFFILAIVIKIDSPGPVFYRQERITRYGKKFKIYKFRTMVINADKDGTLVTV
                     KNDDRITRVGKVIRKYRLDEISQLINVLVGDMSFVGTRPEVGKYVKSYSPLMLATLLL
                     PAGITSETSILYKDEDKLLHDKNNIDKTYIEKILPSKMYYNLKSISEFSCMNDLKIMC
                     KTIFVIFGKEYHADLEGEREFINQIK"
     gene            8046..>8576
                     /locus_tag="ACG808_002313"
     CDS             8046..>8576
                     /locus_tag="ACG808_002313"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5047913.1"
                     /translation="MIEEIKSSDKELINSIVDIHMRTFTNFFLTFMGKKFLELMYRSY
                     TEYNDSGILVAIQNDKPIGFLAYSGDMSGLYKYMIKKRIIPFGFYSCVAFIKKPNILM
                     RLFRAFLKPSETKRDENYVELASIGVNPKEKSIGVGSMLIDNLKSRIDFSKYEYIALE
                     TDALNNDVVNNFYKKNE"
BASE COUNT         3027 a         1442 c         1768 g         2339 t
ORIGIN      
        1 tttgtggtat gattatatag ggaaaacctg tttataaata tttctatgaa tatattagtt
       61 taattatgct tttaaaataa aaagcgctca ttctgatgtg aatgaatcac atctttattt
      121 atctatcatc aatatttagg agtgaatcta tggaaaagcc gttcgtcaag ttattggcta
      181 ctgtggcggt aggcacaggc gctattgtga tttgcttgtt tggataccat ctcaataatc
      241 agcggcaaca tcatcaacgg atcaattacg cagaatctgc catcaccaat caaaaagaca
      301 ccgtgacctc tttatcaaaa gaagtagata agctgtacag tacaaaagaa aaaattttcc
      361 taaatcctga aataacagaa gaaacagttt caaatttgtc acataaacta tcttctatca
      421 aactttcagc agatgattat gacatcaagg aatcagaatt acctaaagaa gctgctgcga
      481 tccaagaaga aaagaaagca gtcttaacac agttagagga tgcagaatca aaactgaaga
      541 tccaaacggc tgtcaataaa ttatttacta aaaacgtatc aaactggcag caagctgtgg
      601 atgatgtcat catcaaagaa aagttggcaa gtgcagatgt cgcccatgta cgtgaaaata
      661 tgagcttttt taaggatagt gcttggaaaa cggtcgttat gcaatatcta ggattcgctg
      721 atacccaaat tgcacaagtc acgcaactag atcagctatt cgatacgatg ctgaaagacg
      781 gtcaagtaac agctactgct acttacgatc aatatttgac cgcactcagt ctcatcgaac
      841 agatccggaa tgaaaaaata agagccgcat acgcgtcaaa agcagaaaca gtcgctcagc
      901 aaatgggcta tagcaatacg agctattaat aagcgagaat tcaagcaagg ctagcaaacg
      961 gaggaaacta tggcaaaacg aagaagaaga tcaagaggga aaaagatatt tctaattatt
     1021 ttagcaattc ttgtcgtatt agtattagca atcgtaggag taggagcaaa attatatcta
     1081 gatctctcac attctattga aaaaacttac gaatcagtgg aacgaaccca aggagaacga
     1141 acaaaaccag ttgatctgga caaacaagaa tctttctctg ttctattgat ggggattgat
     1201 acgggagatt taggtcgggt cgatcaagga agatcagata cgatgatggt tgccacagtc
     1261 agtcctgaag acaaacagac aacgattgtc agcattcctc gtgatacgta tgtcgacatc
     1321 gttggacatg acacaaaaga caagatcaac cacgcttacg cattcggcgg agctgccatg
     1381 gcgatggata ccgtacaaaa atatgtagac atcccaatcg atcactatgt ttcaatcaat
     1441 atgaaaggtc taaaagaact cgttgatgca gttggtggta tcgaagtcaa taatgacctc
     1501 accttctcac aagatggcta tgattttacg atcggtaaaa tcacattgaa cggggatcag
     1561 gcattgtctt attctcgtat gcgttatgaa gatccaaatg gtgactacgg cagacaagaa
     1621 cgtcaaagaa agatcgtaga aggtgtagcg aaaaaagtgt tgagtctgaa cggtgtaagc
     1681 aactatcaat ccgttttgaa tgctctagag tcgaatatga aaacagacat gagctttgat
     1741 gaaatgaaaa agattgcttt agattatcgg gatgcatttg gtacgatcaa gcaagatcag
     1801 ctgcaaggag aaggcttcat gcaagacaat atctcatacc aaaaagtcag cgatcaagaa
     1861 cttgatcgtg tgaaaaaaga attaaaagat caaatgaatc tagagaataa ataggaaagt
     1921 ggagaaaaaa tggaagagac aatcagcttg aaagaaattt ttgaaacatt acgcaaacac
     1981 gtgataacaa tcctcatctc aatgtttgct ggcttggcca tctcaggcat cgtgacgttt
     2041 ttcatcttga cgccaaaata cagctcacaa gcccaattga tcgtgacact gccgcaatca
     2101 gatactgcaa atgtcaatga tgtcaatacg aacctgcaaa tgatcaatac gtataaggat
     2161 atgatcgtca gtgatctagt actaaatgaa gtcaaagatc gtttagaaac agaagacaat
     2221 gtaaaaatga ctgctggaca aatcaaagat gccatctccg tcaatcagtc acaaaattca
     2281 caaatgttct cgatccaagc aacaagcaca aatgcagtga cggctcaaca aatcgctaat
     2341 acaacgtcac aagtattcca agagaacgcc aaagacgtga tgaatgtcga taaaatctcg
     2401 atcatctcag gtgcagttgc caatacgaca cctgtttcgc caaataataa attgaatcta
     2461 gtgattggct tagtgttagg aatgatggta ggcgtaggtc tagctttctt atttgaatta
     2521 ctcgaccgga cagtaaaaga tgaaaaatat gtgacggata cattgggctt cccgatctta
     2581 gggactgtac cagaaatgtc agcgaaagaa ttgaatgctg gtatccgtaa aaaaggacca
     2641 gctccgacac aaccaaaagc tacaaacgat aaaacagcgc catcacgcag aagccgctca
     2701 agagtctagg aggaaagaaa aatggcacga acacagaaac aaaaagcagc ccaaactcat
     2761 gcagtcagtt tggtcacact cacaaacccc tcttcaccaa tcgctgaaca atatcgtacg
     2821 attcggacga atatccagtt tgcctccgca gctggtcagc aaattaaaac gattgttgtg
     2881 acttcatcag gacctggaga agggaaatcg accactgcag caaacattgc cgttgttttt
     2941 gccaaatctg gtcaacgggt gctgctagtc gatgcagact tacgtaagcc cgtcatctat
     3001 aaaacgttca agttgaacaa cgcaagcgga ttaagtaccg ttcttagttc atcaggaagt
     3061 gtagcagatg agatccaacg aacaccagtc gagaatttgt cgattctgcc aagtggacct
     3121 aaaccaccaa atccatcaga actactaagt tcgccaagaa tggatcagat tttagcagaa
     3181 gcaagacaac tatttgatgt agtgatcttc gacatgccgc cagtagttgc cgtaacagat
     3241 gcacagatca tgtcttccaa aacggatggc accttattgg tcgtacgtga aaatatgtca
     3301 cggaaagaat cattgactaa agcaaaagaa ttgctggata tggtacaagc gcgagtcctt
     3361 ggtgttgtat acaatggggc agaacacagc aaagatgctg gctattacta cgggaattaa
     3421 gaaataaaaa gagaggagac aagagaaatg atcgatctac attgccatat cctgccagga
     3481 atcgatgacg gagcagaaaa catagatgcc agtatcgcca tggctgaaaa agcgattcaa
     3541 caagggatca cacatatttt atgcacaccc catcataata atggaaaata cagcaatgaa
     3601 aaatcacaag tcatttctct agtcgcttcg ttacaagcag aattagagaa aagacaattg
     3661 cctttgacgt tattggaagg acaagaagta cgtattaccg gaacactgat cgaagacatc
     3721 gaccgagatg aaattttatt tacagattta gatgatacat atcttctaat cgaatttccg
     3781 acactggaag tgccgttata tgcagaacag ctcttttttg agttgttaca actaggaaag
     3841 acaccagtga tcgtccatcc agaacgcaac gcgcattttc gtaaagaccc gaatcatatg
     3901 ctgccattcc tagatatggg atgcttggcc caactcaccg caccaagtta tgtcggtgta
     3961 tttggaaaag acatccagaa aacagccaaa atgatggtca aacataatct tgtgcagatg
     4021 gctgcttctg atgcccatgg ggtctctaag cgcacctttt acttaaaaga gtgctataag
     4081 cagattgcca aggactttgg taaggaaaaa gtagtacaga tgaaacaagt cgcaaaggat
     4141 ctgatcaacg gggatgaaat cagttatcca gtctatgaag agataaaaaa gaagaagttt
     4201 ggtttatttt agggggagtg taaggtgttt gtattaacaa gaagaagaaa aattgccaca
     4261 ttggttgttg tggacagttt gttattgatc gctgcaaata ttgcagcagc taaattcatg
     4321 aagccgtttg tagctatacc aatggacctt attttaatct ctaccggttt gtcgattggt
     4381 ttttatttat tttatggtag tttattcaaa gttttcactc ggatcaatcg ctatacaaat
     4441 ttaagagaga tcattgcaat ttttggttca ctatctgcaa gtgacgtctc aagtatcctc
     4501 atactgctgt tcattaatcg gcaatacagt ttacggttag ttatctttgc ttatctgctt
     4561 tcgctcttat tgatcatcgg tagccgattg atctggcgta tctacatcga aacaaaaaat
     4621 atgagatatg tatcagcgga ttctgctaaa aatacattga tcgttggtgc tggcgaaggc
     4681 gggcgtattt tatacaacag ctttctggga tccaaaacgg ctcaagatat ccaagttgtc
     4741 ggatttgtag atgatgatcc gaataaaaga aatacgtatc tttcaggaaa aaaagtatta
     4801 ggtgcattaa aagatattcc ggaattgatt gaaaaatatg acattcaaat ggtaacgatc
     4861 gctatccctt ccttatcaag aaaaaaatta cgaagaatct tcgagttagt cgaatccgct
     4921 cacgtgaaag tcaacacgat gccttctata gaagaacttg cttcaggaaa aattagcgta
     4981 tcaaaattaa aaacaattga cgtagtggat ttactgggaa gagatgaggt aaagctggat
     5041 attgaatcca tcaaagatca gatcacagac aaagtcattc ttgtcaccgg cgcaggagga
     5101 tcgatcggtt cagaaatctg tcggcaaatc attcaattca atcctgcaaa gctattgctt
     5161 ttaggacatg gggagaactc gatctacttg attgaccgtg agttacgcac gcatcatcaa
     5221 aattgtccta cagaaatcgt accgatcatc gcagatatcc aagatcgcga gaaaatcaat
     5281 gagatcatgg aacaatatca tccagatatc gtctaccatg cagctgctca taaacatgta
     5341 ccactgatgg aatataatcc aaaagaagca gtcaaaaaca atattttcgg tacaaaaaat
     5401 gtagcagaag cagctaaagc agcaaaagtg aaaaacttcg tcatggtatc gacagataaa
     5461 gctaacaatc ctccaaatgt catgggttca acaaaacgaa tcgctgaaat gatcgtgact
     5521 ggattgaatg aagaaggctg tacgaaattc tcagctgtca gatttgggaa tgtccttggt
     5581 tctcgcggta gtgtgattcc agtattcaga gaacaaattg ctaaaggtgg tccaatcact
     5641 gtaacagact ttcgtatgac ccgatacttc atgacgattc ctgaagctag tcgtttagtt
     5701 attcaatctg gcgcattagc aaaaggtggc gaaattttta ttctagatat gagtgaacca
     5761 gtgaagattg tagacctagc caaaaacatg atccgattga gcgggtatag cgaagacgaa
     5821 atcgaaatca ttgaaacagg tatccgtcca ggggaaaaac tatatgaaga actactgttg
     5881 gataaggaaa gaaatgatga agcagtttat gagaaaatct ttgtaggaaa catcaaaggt
     5941 tactcaatac aaaaagtgat ggattttgtc aagagcctgc cgcaagacga tgaacaattg
     6001 gctaaagata tcgtcacttt tgcaaatgct tcgaataaat aggggggaaa tataaatgaa
     6061 taaattagaa aaacgtcaga taccattttc accgccagat attactgatt tagaaataaa
     6121 aaacgttgta gaagtattaa aatctggatg gataactacg ggaccaaaga caaaaaaatt
     6181 agaaaaaaaa ttacaaagtg gttaggaact tctaaagtag tttgtttaaa ttcacaaact
     6241 gctgctgcag aaacagggtt acgtttgctg ggaataggag aaaatgatga ggttatcgtc
     6301 ccagcttata cttattctgc cagtgcatcg gttgtatgcc acataggcgc gaacctagta
     6361 ttaatagact gtcaacaaga ttctttggag atggattacg atgcattaga agcagcaatt
     6421 acggagaaga caaaagtgat tattcctgtt gatcttggtg gtataccttg tgactacgac
     6481 cgtatttttg agattgtgga gaaaaaaaga aatttatttc aacctagtaa tgagattcag
     6541 aaagcttatg gtcgtataat tgttatggca gattctgctc atgcatttgg agctaattgg
     6601 catgatcaga tggttggtaa tgttgctgac ttgacaacat tttcatttca tgctgttaag
     6661 aattttacaa ctgcagaagg tggagcttta gtatggaaag agcacgacaa actagataat
     6721 gaggcaatgt atcatcagtg ccagctgctt tctttgcatg gacagtcaaa agatgcactt
     6781 actaaaacac agttgggtgc atgggaatat gatattgtgg gtccatggta taaatgtaat
     6841 atgactgatg ttactgcatc aattgggttg gcacaatttg aacgctatcc taatattctg
     6901 aaaagaagaa agaatataat taagaggtat gatgaagcct ttcaacaact aggaattgaa
     6961 gttcttatgc attatacgga tgattatagt tcttcaggcc atttatatat tactagagta
     7021 cctaacatta gttctgagca aagaaatgaa ataattataa aaatggcaga acaagggatt
     7081 gcttgcaacg ttcactataa accacttcca atgtttacag catataaaaa tatggggttt
     7141 gatattaata actatcctaa tgcctatgct cattttgtaa atgagattac attaccattg
     7201 cacacgaaac ttacagatga agaagtagaa tacgtaatta ataactatag taagattgta
     7261 aaggatttgg ttggatgata ggagaggcaa aatgagaaag tgggaagatt tgccaaaaga
     7321 aatgcagaat gatgaaatac ggtattatta tgatatcctc agtaaaaaaa aattcagctt
     7381 agtggtaaaa agagtatttg atattttagt gtctgcaact ttgttactga cactttcatg
     7441 gttatttttt attcttgcaa tagtgataaa aattgattct cctggacctg tattttatag
     7501 acaagaacgt attactcgtt atggaaaaaa atttaaaatt tataagtttc gtactatggt
     7561 aataaatgca gataaagatg gcactcttgt tactgtgaaa aatgatgatc gtattacaag
     7621 agtaggaaag gtaatccgta aatatagatt agatgaaatt agtcagctaa ttaacgtttt
     7681 ggtcggagat atgtcttttg taggaaccag accagaagtt ggaaaatatg taaagtcgta
     7741 ttcaccatta atgttagcca cattattatt accagccggg attacatcag aaactagtat
     7801 tttatacaaa gatgaagata aattgttgca tgataaaaat aacatcgaca aaacttatat
     7861 agaaaaaatt cttcccagta aaatgtatta caatttgaaa agtatcagtg aatttagttg
     7921 tatgaatgat ttgaaaataa tgtgtaaaac tatttttgtt atatttggta aagaatatca
     7981 cgccgactta gaaggtgaac gagagtttat taatcagata aaatgaatgg aggataagag
     8041 ttttggtgat tgaagagata aaaagttcgg ataaagagtt aattaattcg attgtcgata
     8101 ttcatatgag aacatttaca aatttttttt tgactttcat gggaaaaaag tttttggaat
     8161 tgatgtatcg ttcttacaca gaatataatg attctggtat attggttgct attcaaaatg
     8221 ataagccaat aggatttctt gcatattctg gagatatgag tggtttatat aagtatatga
     8281 taaagaaaag aataataccg tttggatttt atagttgtgt agcctttata aagaaaccca
     8341 atatccttat gaggcttttt agagcttttc taaaaccaag cgaaacaaaa cgtgatgaaa
     8401 attatgtgga attagcatcc attggagtta atccgaaaga gaaatcgata ggagtaggtt
     8461 ccatgcttat agataactta aagagtagaa ttgatttttc aaaatatgaa tatattgcac
     8521 tagaaacaga tgcattaaat aatgatgttg tgaataattt ttataaaaag aatgag
//
LOCUS       DAZUMK010000110         8511 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000110 DAZUMK010000000
VERSION     DAZUMK010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.112"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            84..266
                     /locus_tag="ACG808_002314"
     CDS             84..266
                     /locus_tag="ACG808_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047914.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            250..435
                     /gene="tnpB"
                     /locus_tag="ACG808_002315"
     CDS             250..435
                     /gene="tnpB"
                     /locus_tag="ACG808_002315"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFS5047915.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            678..2198
                     /locus_tag="ACG808_002316"
     CDS             678..2198
                     /locus_tag="ACG808_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFS5047916.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
     gene            complement(2385..2957)
                     /gene="hxlB"
                     /locus_tag="ACG808_002317"
     CDS             complement(2385..2957)
                     /gene="hxlB"
                     /locus_tag="ACG808_002317"
                     /EC_number="5.3.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0043800 - hexulose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HFS5047917.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(2964..3626)
                     /gene="rpe"
                     /locus_tag="ACG808_002318"
     CDS             complement(2964..3626)
                     /gene="rpe"
                     /locus_tag="ACG808_002318"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFS5047918.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            complement(3642..4157)
                     /locus_tag="ACG808_002319"
     CDS             complement(3642..4157)
                     /locus_tag="ACG808_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HFS5047919.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            complement(4159..4443)
                     /locus_tag="ACG808_002320"
     CDS             complement(4159..4443)
                     /locus_tag="ACG808_002320"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5047920.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            complement(4477..5805)
                     /locus_tag="ACG808_002321"
     CDS             complement(4477..5805)
                     /locus_tag="ACG808_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFS5047921.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            complement(5819..6274)
                     /locus_tag="ACG808_002322"
     CDS             complement(5819..6274)
                     /locus_tag="ACG808_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5047922.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            complement(6277..8223)
                     /locus_tag="ACG808_002323"
     CDS             complement(6277..8223)
                     /locus_tag="ACG808_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5047923.1"
                     /translation="MIERHVNLLKLLSRQEDFKPASFFSDKLAVSTKTIYQDIELLED
                     YVVNYQVTIERLPSLGIKLIGDRKNVEHLISSVENYLEKDIYSPEKRRVEIIKEVLFG
                     DKPVSLTHLSQSFMVSKTSLYNDLRIINKIIGSGEVKLQSSAEGISFTGNEQAIQRAI
                     KQLVFYYANDMESKTFFQVLNMLFDSKVISVIHDLLFNDYSELTEKVSDYYVRSLIST
                     LVIQCNRLLKGHHLTVEEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLC
                     RQLFAHRVTNNLKTSNEEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLR
                     LKKGIKIQNPLLENIKSQYSELFSIVWYAMSILEEEFDVTLTDDEVSLILIHFQIAIE
                     DQSKANNIIIICQYGISSAQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISS
                     LSFDAGGIPLVRVSPLVSKTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKF
                     VQSSLVKTGVQVESKEECLDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALP
                     HANPSMVIESNIFIVALKNAINWGDRKVKLVIMVSLAEEDVSEIRGVYEELYQFIGQK
                     EMVDRLLQVHDSEKLLRIFQQKGE"
BASE COUNT         2827 a         1590 c         1343 g         2751 t
ORIGIN      
        1 taagcgtgtc ataggatagt atctcgtacg ccgattatct ttcttctata attaaactat
       61 caaatataga aggagaaaaa cgaatgaata aaaaagatat tgtaccagtt caattttcaa
      121 gtgcggctca aaaatcgcaa attgacacca agccaaaact agcttgcaga atcaaatcgg
      181 aagataagga aatatttatt tataatggaa tggataaata tcttttgtat accttgttaa
      241 aagagttgaa tgccaatgct cattgatgca actgcattga aacatatttt tatcgtttgt
      301 ggaaaaacag atcttcgaaa aggaattgat ggactagcag ctttcgttat agaagaatat
      361 gaccttgatg tttacgatca agcgcttttc ttattttgcg gtacgaagaa agatcgcttc
      421 aaggctcttt actaggatca aaatggattt gtattaatgt acaaaagatt tgaaagtggg
      481 taccttcaat ggccgcgttc cagaaaagaa gtcttactga ctggcctttc aatcgaagaa
      541 aaatgtacgc taaaagaaac gaaaaaaggt gcgatatatt agacgaaaac cttgttaaat
      601 caaggatttt gtctgttttt cgttgtcata aaatggtata atagatgtaa gaaaatcgct
      661 agaatggtgg tgtaaaaatg acaaaaatgg aatctgaact tcttgtacaa atcaagcaat
      721 taacacagtt gcttgaaatc cagcaacagg aaaatactct tcttagagaa caaattgctg
      781 aaatgaatcg acgcttgttt ggacgaaaaa aagaaacacc acccgtagat gggcaaatcg
      841 atttattaga cgactcaact tttaatgaac cagagcacac tggacaagaa agccaggaac
      901 ccattacggt atcttctttt aaacgcagaa aacgcaaggg actcaaagca ctttcattag
      961 aaggtctgcc tgaagttcct attcattatg aattaaaagg tgccgattgt ttgtgtgaag
     1021 actgtggtca tactttacat gatatcggtg cgacaaggct tagagaagag ccattgtatg
     1081 ttcctgccca tatagaaaaa caagtcatct accaacatgc ctatcattgc agagaatgtg
     1141 aagcattagg agaaacaaaa atcaaaaaag cgcctgttcc taaaccgttg atcaataata
     1201 gtctaggttc ctcttcaatt gtgacacaga gtatttggga aaaatttgtt aaaaaggtgc
     1261 cggcatatcg acaagaaaaa gaatggcatg cacttggttt ccctcttgat cgtaaaaaaa
     1321 tcactaattg gcatatcaag gtaagtgaaa tggttttaga gccaatctac aattgccttc
     1381 atcaagaatt aagcaatcag gaggtcttac acgcggatga gacaagctat cgggtattga
     1441 atactcataa agaaaagacc ttttactggt tatatgcttc aggtaaaagc gaagaaaaac
     1501 aagttgtgct ctacgagcac gcaaattcgc gttccgttga tgagccaaaa agttttttag
     1561 aatcatttag cggtttcttg catactgatg gttacgcggc ctatggaaag ctgccggtaa
     1621 acaatgttta ttgttgggcg catgttcgca gaaagttttt tgaagcattg ggtaaacaac
     1681 cgtccccaaa aagtcatgcc aaaataggat tagacttctg tgacaagatg tttcgaatcg
     1741 aacggacctt taataagctt agcgcagaag aaaggtttgt agcgaggaag gaaacgttgc
     1801 agcctgtgat gactgaattt tttcattggt gtgagagttt tgcttatttg ccaaaatcgc
     1861 agcttggaaa ggcggttcaa tacgcagtca atcataaaga cggacttcag attgtgttgc
     1921 ttgatggacg cttggaactt tcaaataacc gagcagagcg tgccatcaaa gagttagtga
     1981 tcggtcgaaa aaactggcta ttttctaaaa gcttaaaagg cgctagatca aatggaatta
     2041 tattgagtat tatccagaca gctgttgcga atgggttaaa catccgcaaa tatttaaatc
     2101 atctttttac agagattccc aatctgtctt cgatgacacc tgaagcgcta cgcgcttatt
     2161 tgccatggaa tcagcagata caagaaatct gtaaataata taagccacaa ataacctgat
     2221 ttgagtgtat caaatcgggc tatttgtgtt taggtaccat tctatgacac gcttacgctc
     2281 aaaactactg aattattaag ttatgtctaa tcaaataaaa aaaccattct catatttgct
     2341 gaataattag catttagaga atggtttttt atctttaaaa tctactactc aaaatctgca
     2401 tgtcgagcaa acatcgtttc tgagctttca cctgttttat ccattaaaga taacactaaa
     2461 ctatcaaagg tgagaaatgc taattgttca aaacttgaac ccataggttg tgaaaaagta
     2521 tcttcttctc gttgattttc atctttggta atacctggta atacaagaac agaatctgct
     2581 aattgaccaa tcgacgagtc ttttttcatg gtaactaaag caatgcttac accactttga
     2641 tttgctttgg ccgctaaatt ttttaaacta cctgtttcac ctgagccaga acagataatt
     2701 aataaatctc caggttgcga atgtggtgaa ctaatttcac cgacaaggct gactgaaaaa
     2761 cctaaatgca ttaagcgatt cgtaaatgct tgcatggcaa ttccagagcg tcctgcacca
     2821 gctagaaaga tgtgtttagc ttgaacaatt ttctcactaa attgttctaa ctctacagaa
     2881 ttaatacgtt ttgagttatt ttgtaattct aagataatcg cttctaaata gtttttattt
     2941 tggtctttat tggtcatgat cttttacttc acttcctcta atgattgatt tagtttatca
     3001 atttgttctt gcggggtatt tttcccattg aagatgaaac cgcctgatac aaaaatatcc
     3061 gcccctgctt tagaacaaac tttaatcgtt tgattatcaa tcttgccatc tacttcaata
     3121 tcatacgtat aatcattttg tttgcgtatt acatctaact gttcgatttt aactactgtc
     3181 tcttgaataa aatgcttatt ctctgttcct ggattgattg tcattactaa tacttgatcg
     3241 actaaagata atactggttc aataaaactg actggtgttg caggattaaa agtaatccct
     3301 acttttgctc ctgcttcctt gactaactga atgaccttca ttgcgtgttt ggtagcttca
     3361 atgtggaagg taacaatatc tgctcctgct tctacaaatc ttgtgacaat tttttctggt
     3421 gtatcaatca tcaaatgaac atcaaacaca acgtctgtta gtggacgaat atctttaata
     3481 acacttggtc caaaagccat atctggtaca aaacagccat ccattacatc aatatgcaaa
     3541 taatcaacat tcttttcttt taagatttca aataactctt taacttctgc gataggtgca
     3601 tttaacaatg atggagcaat tgtcattctc tattcccccg tttattctcc cataatcatg
     3661 agattgattg ttctagttcg ctctcttagt tgatcccaat cgacaaaata gacacggccc
     3721 atttctccta ataaaagttt accttcttga acaattaaag tcattgagtt ggttccataa
     3781 atagatgaac gtaaatgtgc atcggtattt agcatgaccc atttttctgc agggtaattg
     3841 ggatcagata atcttgttcc aaaatcaatg tgttttggcc ccggactgtt gtattgattt
     3901 tcactcgtca tttttggaat aatcgtttcc attacattgt tgatgtctac atgtaaataa
     3961 tcatcaccaa aaaagtttgt gtcatgcatc gtttcttcaa aaaagagtga gcaagttgta
     4021 tgcgggctcg aaattactaa cattccattc tcaatacctg ctgtatcaac tgtatctaaa
     4081 agttcttttc gaatatcatg atacgaagga cgccctgaaa ctgtttcaat agtcaaagtg
     4141 tcatgatgaa aacgcatatt aaacacctaa tctttctttt acatctgcat aaatggcatc
     4201 tgtaccaata cctgttaata agcccaagcc attcgttact ttagctttaa ttacatcagg
     4261 gacaattgtt gtacttacta cagcatcata ttcatcaatt ttatttagct catcagcaat
     4321 tttagattga taaattttca cttgatctgt taatccttct tcttccaacc attttgataa
     4381 ttttgctact gcaattgtag aagtagcatg acctgctcca caaataacta ataatttttt
     4441 catattgatc tcttctttct tattttaatt ttatgattaa gctttcttca ctttgttcat
     4501 gtagatagaa gcacctacta aaacaactcc taatccacct attccaatta caccaaagta
     4561 agatgctaat aaaacaaaca accctactgg ccataatcca cataacaaca tagttactaa
     4621 tcctgctgtc cccacatcga aacttgctaa tgtaaacaca tcagtgataa ttggtgccaa
     4681 ccatgttgcc atatataatg ttgtaacaac atacaacgaa catcctacta atgatctcac
     4741 aatatttcct ttaaaataag gaatcattaa acagatggcg aaagcaaaga atgctaaatc
     4801 tccaaaaggt aatactttat ttcctggaag aattagtgct agcaataatg agattggtac
     4861 aagcaataga gaagttgaca taatagttgg atgacccaca gttaacgctg catccattcc
     4921 aatgtttact tggcgaccat ttaacttttt attcgtaaat tctttcgccg cttctgcaat
     4981 tggcattaaa ccttccataa acattgctac cattttaggc ataatcttca tcactgccgc
     5041 agtcataatt cctaagtttc caattcctgc tacgtcatag cctgccataa tacccactac
     5101 aataccgata attaggccaa taacaactgt gtcacctaaa acaccgaaac gttttgttaa
     5161 tgtttctgca tctgcatcta ttttattaaa tccaggaata cgatcaaaaa cccaatttac
     5221 tggtttggca aatagtactc ctgataatcc catcatatgt gtgatagaga ttcctggata
     5281 gccgaaaaat tcgctaacag ttttttgtgt acgatctgct aagaaccatt gtaaaattac
     5341 tgcgacaatc gcgccaatta agccaaacca aaagtttccc gtaacagatt gagtaatcat
     5401 acctaggaaa attgcgaacc aaaagttcca aatatcaaca ttcaatgttt ttgtccaacc
     5461 caaaaccaca aatatgaagt taagaattaa tgaaattgga atcattaaaa ttcccaatga
     5521 tgtagagtaa gccaaaggtc caccaacacc tacgccaata tctaggatat tcaggccacc
     5581 accgtattgt tcccccattt gttgaattgc tgttcctaaa ccatctgata ataaacttaa
     5641 aactaatcct aaaccaatag aaccgatacc aacagtaacg ccggaaacaa atgctttacc
     5701 aggttttaat ccaaataata tcccgattac aaaaataatc agtggtaaaa agacgtttga
     5761 ccctaatcca ataaaccatt caaaggcatt taatacaata tccatctctc tattcctcct
     5821 aataaactcc tgcagaatgt actaactcaa taaatgcttc tttcgtggta catgcaagta
     5881 attcatttac tttttcttca ttttgaaata ggctaactaa gttttgtagt gtatcaattt
     5941 gttcatgagg ttgtgacatt gccaacataa agactaaacg tacaggtatt tcatgagatt
     6001 tatctgtcat atctgaaaat accagtggtt cctttaatga ggcaaaacag atttgagagc
     6061 tatttacatg tgcggaatct gtatgtggga tagcaaaccc aatttgattt acttctaatc
     6121 cagttggaaa ttcttgttca cggttggtga taccttctaa gaaactatct ttcacgcaac
     6181 cagcgttttt taattgttca gacattactt gaaacaattc tttttgatct ttcacttcta
     6241 attgaaataa acataattct ttatctagat acatttttat tctccttttt gttgaaaaat
     6301 tcttagtaat ttttcgctat catgaacttg taacaagcga tcgaccattt ctttttgccc
     6361 aataaattgg taaagttctt catatactcc tctaatctca gatacatctt cttcagctaa
     6421 actaaccata atgactaatt tgacttttcg atcgccccaa ttaatcgcat tttttaatgc
     6481 tacaataaaa atgttggatt caatgaccat tgatggattc gcatgcggta aagcaacacc
     6541 actttctaaa ttagtattcc ccattttctc tctagagaag atagaagcac gaaactcttc
     6601 agtaactaat gaattggctt ctagttcgga aatcatcaaa tccaaacatt cttctttgct
     6661 ttccacctgt actcctgttt ttactaaaga tgattgaaca aatttagata tggtgggaac
     6721 tttaaccgct ttaggaatca gttcattttg aataaatcgc gaaggttctt ctattaaata
     6781 tttagtataa gcttccataa tattcatata atctgtttta cttaccaatg gagataccct
     6841 tactaagggt ataccacctg catcaaagga aagagaggaa ataatcaaat caacttcccc
     6901 aatttcagct aaatgaaact tatctaaagt tgatatttct acattatctt ttgctggtaa
     6961 aaaccgtcga actttattgt aaattaattg cgcagaagaa ataccgtatt ggcaaataat
     7021 gatgatatta tttgctttgg actgatcttc aatagcaatc tgaaaatgaa tcaaaattaa
     7081 ggaaacttca tcgtccgtta aagtcacatc aaattcttct tcaaggatgg acatggcgta
     7141 ccatactatg ctaaagagtt ctgaatattg agatttgata ttttctaata acggattttg
     7201 aattttaata ccttttttta aacgaaggac cattgctggc acatgataca ataaggattg
     7261 atataatcga tcattttgag tcaaatcaat tttttcaatc tcactcattc tttcaatcaa
     7321 tttatgaacg ctcatagcgt actcttcatt gctggttttt aaattatttg tcacacgatg
     7381 tgcaaatagt tgacgacata gatattcttt atctgcgtca taaaatggaa gattcaattt
     7441 tgccttcaat tcttcaatta aaccatttgc cacaatgtac gtttccatat agcgaatgct
     7501 attgaagagg aattcttctt caacagttaa atgatgacct ttcagtaaac ggttacattg
     7561 gataactaaa gtagaaatca atgaccgaac atagtaatca gaaacctttt cagtcagttc
     7621 actgtaatca ttgaatagca aatcatgaat gacactaata acttttgaat caaacaacat
     7681 atttaaaact tgaaaaaatg tttttgattc catgtcattc gcataataga atacaagttg
     7741 cttgattgct cgttgaattg cttgttcatt accagtaaaa ctaattcctt ctgcagatga
     7801 ctgcaactta acttctccac tgccaataat tttattgata attctcaggt cattgtataa
     7861 cgatgtttta ctaaccataa aactttgaga caaatgagtt aaagaaacag gcttatcacc
     7921 aaacaaaact tctttaatga tctcgacacg tcttttttct ggagaataaa tatctttttc
     7981 aagataattt tctaccgatg aaatcaaatg ttcaacgttt ttcctatcac ctatgagttt
     8041 gatacccaaa gaaggtaatc gctcaattgt tacttggtaa ttaactacat aatcttccaa
     8101 caattcaata tcttgataaa tggtcttggt cgacactgcc aatttatcac taaaaaaaga
     8161 agcgggttta aagtcttctt gtcgacttaa taattttaat aaatttacat gtctttcaat
     8221 caaacttgtt cacctcttaa cgctagcatt tgttacctct tttcattatt tattctagca
     8281 ctggaacccc cttccctcaa ttacatctat gtcaataata cttgtggaag tttgttgtaa
     8341 aggaaatcca aataatctta aaaaatgaga aacaaaaaag gagaaatcct taaaagagct
     8401 aagatattcc aatagaaaat actccttatt atggaagtaa tatgaaatat tacaattgaa
     8461 aaataaaaac aatataatgg cgtcactaaa attcgtattt ttttattata a
//
LOCUS       DAZUMK010000111         8428 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000111 DAZUMK010000000
VERSION     DAZUMK010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.113"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(181..792)
                     /locus_tag="ACG808_002324"
     CDS             complement(181..792)
                     /locus_tag="ACG808_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS5047924.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(915..1040)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(1122..1838)
                     /locus_tag="ACG808_002325"
     CDS             complement(1122..1838)
                     /locus_tag="ACG808_002325"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFS5047925.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(1844..2413)
                     /gene="scpB"
                     /locus_tag="ACG808_002326"
     CDS             complement(1844..2413)
                     /gene="scpB"
                     /locus_tag="ACG808_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFS5047926.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(2397..3167)
                     /locus_tag="ACG808_002327"
     CDS             complement(2397..3167)
                     /locus_tag="ACG808_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFS5047927.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(3164..3535)
                     /locus_tag="ACG808_002328"
     CDS             complement(3164..3535)
                     /locus_tag="ACG808_002328"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5047928.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(3554..4441)
                     /gene="xerD"
                     /locus_tag="ACG808_002329"
     CDS             complement(3554..4441)
                     /gene="xerD"
                     /locus_tag="ACG808_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFS5047929.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(4537..5010)
                     /locus_tag="ACG808_002330"
     CDS             complement(4537..5010)
                     /locus_tag="ACG808_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFS5047930.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(5115..5978)
                     /locus_tag="ACG808_002331"
     CDS             complement(5115..5978)
                     /locus_tag="ACG808_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFS5047931.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(6174..8273)
                     /locus_tag="ACG808_002332"
     CDS             complement(6174..8273)
                     /locus_tag="ACG808_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFS5047932.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
BASE COUNT         2162 a         1767 c         1434 g         3065 t
ORIGIN      
        1 taaacaaaca ttttcgtctt ttaaaaggag aagaatttca catttgcaaa gaattgattg
       61 atttttatgc ataaatcaaa aaaatactca aaacaaacaa ataaaacgtt tacattattg
      121 catgtcttgt atttttttcc aaaaaaaaaa gaccaagaaa tttcttgatc tttttgatat
      181 ttatttgact gctgtatgac gttgttcgaa ctgatgttgt tttcgactta accatggaag
      241 aagttgcgca tgcagcacta aaaagacagc actcacgatg aatcctttga tcaagttgaa
      301 aggaacgatc ccaatcagta catagtttgc taaagacatg cccagcatct gtgttgcgtt
      361 cagccctaaa aacattaagt aaagaggcgt aataacgaaa taatttgcga tactcataaa
      421 aatagttaga gcgatggttc ctgtaatgac acctaaaaat ttgtttcttc ctttatgctg
      481 tttctttaca ctaaagaaat aataaatcgg gaatgtaaag atcgttgttg ataagaaact
      541 agcagtgtct cctaccaaat tatccggaga aaaacctgta gtcagcagat gtaatactga
      601 acgcaagaac gctgttgcaa ctccggccgc tggaccgaat aagaacatgc tgatcaatac
      661 cgagatatca ctaaaatcga tcttcaaaaa actaaaagcc ggcatgatcg gaaaagcaaa
      721 atactgtaaa actaatgcga tcgcagcaaa catcgctact gcaaccattt tctgtacacg
      781 ggtgttcttc atttgaaatc ctcctgttag gactcctctt cagcgaactt tttgacgttt
      841 ctctccaaat gaaaaaactc tattttccag ggaaaataga gttagagtta tttgatcaag
      901 cgtcgtcaaa taagctctat cttctttatc cagactatac tgtcggtatc ggaatttcac
      961 cgattcagct atttttttgc aaaataggtc gtggactata accaccggtc gggaatttca
     1021 ccctgcccct gaagacgaac cttatataat ttttgtttac atattcatta tacatatcta
     1081 ttcaaaaaac gcaacatcta acacttataa aaagtaagtg ttcattttga gtggttcagc
     1141 aaactgctga cttctttttt gttcagttca cggtaatccc caggacgtag gccttgtaaa
     1201 gtcagatctc cgtatttttc acgttttagt ttttgcacag gaagaccaac tgcttgaagc
     1261 atatttttca cttgatgatt acgtccttcg tggatcgtca attccacgat actgtggttt
     1321 gtcttaggat ccactgataa aatttggaat ttagctggag atgtttttcg tccttcgatc
     1381 tttattcctt ttgtcaaagg tgcaagcatc gattttgtag cgatcccttt tacctttgca
     1441 acgtaaactt tatccacttc atgttttgga tgtgtcaggc gctgagcaaa atctccatca
     1501 tttgtcagta gcaagatccc cgaagtatcg taatccagtc gacctacagg atagatacgt
     1561 tcttttacca tcggcaaaag atccgtaaca acttttcttc ctttgtcatc tgcaacagca
     1621 gaaataactc ctttaggttt gtacaataaa tagtaaccgt attcttcttg ataaattggg
     1681 acttcatcca cttccacagt gtcatgtttc ccaactttta tccctaattc ccttacaact
     1741 tgtccgttta cttttacacg tcctgcaagg atgaattctt ctgcttttcg acgagaagtg
     1801 atgccagcgt gggcaattac tttctgcaat ctctccatta caattattct cctttattca
     1861 ttccatcaaa gaatagatct ggtgacacgt cttcttcaag tgaaatttcc atttcttgtg
     1921 tatcaggcag ttcttctaat gatttcaaac caaaataatc taagaagtag ccagttgttc
     1981 catataatat cggccgacct ggtccttcta ctctaccttt ttcttcaatc agtttatggg
     2041 cagctagacg ctgtaccgct cctgaaacct gaactcctcg aatctgctcg atttcagctc
     2101 ttgtgattgg ctgtttataa gcgatgattg ctaaggtttc gacggcggct tgagataatg
     2161 tgtttgatgt cccttgcgca tatcgcttaa gtaagggagc cagctctttt ttagtcgtta
     2221 atacataatg gctttccgtt tcaaaaatat gcaaagctga gtgcgagttt tgagtgtaag
     2281 attcttttaa ttctttcact aaacttgtga cttctgcttc gctgagattt aacagtccgc
     2341 ttaattcttc attactgatc ccttcctcgc caacaataaa aagaagggct tcaagttcac
     2401 ttaatttcgt catgttcggt atctcctact gctggataaa gcataattga ttcatagttt
     2461 tctttttgtt gcgcaatgat ctgccctttt ttcatcaatt ctagcaaagc cataaaagta
     2521 gtgacaactt cacttttaga aggtgactca aaaaaagaat cgaaagtaca gccatgcgca
     2581 tttcttttta tacgctcttc gataaatacc attcgttctt cgatcgttgt gtcatcgggc
     2641 gtgatcgttt tttccacttt ttgagaaaat ttcttacgtt ctaagatttt ctgcatggca
     2701 cggatcaaat cgattttttt gacttgtcct cgcttcaacg gcttttcagc atccgctaac
     2761 ccatcgatgt tcataggttc ttttgtgtaa taaagacttc gttcctctgc tttttctgtc
     2821 agtctttcag ctgcatattt atatttacgg tattctaaaa gctgagtgac caattcttct
     2881 cgaggatctt cttcccataa ttcttcatcg tctgtgacga attcttggac aggaagcaat
     2941 gtcttgcttt tgattgccat caatgtagca gccatcacta agtattctcc tgcaacagct
     3001 agctcgagtg ttttcattgt atggatataa ttcatatatt gctccgtgat cgtagcgatc
     3061 ggtatatcat atatatcgat ttccatttgc tgaatcagat gaagcagcag atcgagagga
     3121 ccctcgaata catctaattt aatattgatc tccgtcaact cattcactct ttcttctttt
     3181 tctttgctag ccattttcca atgtaaggct ctgtgatttc agttgcacgg atggagacat
     3241 ccgggtactt ttcctgtaat tcatctaaca tttgaaagcc catattctca ccgcgataag
     3301 acggattcaa agaaatgtca tggatgatca gctgatcgtt cgttgcccag taaataccaa
     3361 taatcccttg aatgttggtt gaaccttctg gcgtaaagaa aaaaagttca taatcgtctt
     3421 cagattcgta cgtatcaatc tccttcaaca aagaagtttt acctgtcagt ttcttatgga
     3481 aactcaatag tcccatagca attttacggt tttctttccg gtaatgtgtt aacatcttct
     3541 caccttccta tgactaagcc cttggaaaat gctgcttata aacatccgtc attcttttct
     3601 tcgtgatatg tgtataaatc tgagtcgtag agatatccgc gtgacctaac aattcctgta
     3661 ctgtccgcaa atcagctcca ttttctagca gatgtgtggc aaagctatgc cgcagcgtat
     3721 gtggtgttac ttctttgtaa atcccagcct ctcgtactaa ctgcttcaga tttttccaga
     3781 ttccttgccg agaaagacct ttcccatgat tattgacaaa tacatgcatt tcagatgcgt
     3841 cctttacaag taatggacgt gcttcgtcta agtagcgttc cagccattgg atcgcataat
     3901 cacctaaagg aatgatccgc tctttatctc cttttccaac tgtctgtacc agtccaagag
     3961 aaagatgaag atctcccaat ttcaatccaa tcaattcact gacgcgcatc ccagtagcat
     4021 acatgacttc caagattgca cgatctctta tccctaacgt ttttgtcgta tcaggtgctt
     4081 caatcagctt ttctacttct gtcagagtca atgtacttgg taatttctgt gctttcttcg
     4141 gcgtatcgat atgttgcatc ggatcatgat cagtgatacg ctcttgccgt aaaaactgat
     4201 ggaatcttct gagacttgag atcatccgag taacagttgc tgtcgctttt ttttcttcat
     4261 gcaacttctc cagaaaacgg atgactaaaa agcgatcgac atcctgccat gaagcaattt
     4321 tctgctcctg tagaaaatgc aagtactgtt ccaaatcacg ttcatagctt tgtcgtgtat
     4381 tggcggacaa tccccgttca atattcaagt agtggagata atcgttgatt tgctctttca
     4441 taataatccc cctcatgcct ctctatcata tcagataccg cttcattctg atagtctttt
     4501 tccataatct gagcaaagaa aaagccgtct agacactcag acggactggt ttcttttttc
     4561 tttttcatag cattcctgac agattccatg gaacgttagc cgatgatctt ttactaaaaa
     4621 atgataacgt tcttctacta cttgctccac ttcaagtaaa agatcctctt ctacttcctc
     4681 gatcgtgccg cagttcaagc atagtaaatg atgatggaaa tgtttggcgc cttcttttct
     4741 caaatcataa cgggctaatc catcattaaa acttacttta tcgacaattt tcaattctgt
     4801 caagatttca agggttcgat aaacagtagc aagtccaatt tccggactct tttttttcac
     4861 taaaaagaag atttcttctg cagataaatg ctctttctca ttttccaaaa gcacaagcag
     4921 agtagcttct ctttgcggtg tcagcttaaa ccccgcttca tgtagctgct gttttgtttt
     4981 tttcatcgcc gaaatagcat tcatgaccat ttactcaacc ccaacaattt ataataatta
     5041 caaataaaaa aagatataac actatgttaa ttaaaattat tataagctag ttaagaaaaa
     5101 catgcaaggg attctcaatt agattttttg actagttttg tcacaccgtt ttcttgtgta
     5161 atcaatcctt gtttcaaaag attacctaat gcgcgcttaa atgctccttt gctgatacca
     5221 aaagctttca tgatttcttc tggatccgat ttatctgtaa agttgatttg ctgatctgca
     5281 gaacgttcta aaatggttaa aatcatttgt gcatcatctg atatagcttc atatgctctt
     5341 ggcttcaatg aaagattcag tacaccgtct ggtcggacac caatcactcg accttttatc
     5401 tgctcgccta gacgaggttc attatatcgt tcagaaggat ggataaaccc aagatagaaa
     5461 tcttccgtta aaacgtacgt acctgttaat ttcaagcggt atgctgttcc tatcacatct
     5521 ttgtttttca tttcttctgt tccatatttg cttaaagatt tgaatatttt ttcatctgct
     5581 aaactgcccc aaatacggtc ttttgcatca actctaaggg aaatcatcaa gcgatcccct
     5641 tttttaggcc atagctctgt cattgtcggc aattcatcaa gagatacgac aaagtcttta
     5701 tctggaagac cgatatcgac aaacgcacct aaatcttttc gtgtagcagt aacgattcca
     5761 aaagcgtaat gaccgatacg gcttttagga atttgtgtag tgatccgatt ttcatgtttt
     5821 tgattttggt aaccgaatcc ttcaactgct tcaccgattt tgtgttcacc ttctgatttg
     5881 cttaagccaa aggtttgtcc gtttttttgg acaaagtagt ttttttcatt ttcatcaata
     5941 ataatacctg taaatatagt tgctaataaa tcgttcattt ttttctccct ctatagtgaa
     6001 aggttttctt aatacatttc tttgctatca taacataatg ttatctactt gaatagctga
     6061 gccttggttt ggaaaggtga aaaaaggagt atgacaacag ttgtgtcata ctcccctttt
     6121 tcgaataaac ggtgttaaac ggcaggctca cagcctcttt catttgccta aaattaattg
     6181 ctcccatttc ctccgaacca tccagtttta cagttgatgg ttacgatgta aaatggcaaa
     6241 gcactttctc caatgccttt catcaatgca gattttgttg aagtggcata tagattataa
     6301 tagccttccc cattttcgat tctggctttt tctaatctgt aacctccttc aacgatatat
     6361 ccacgttgga tacatgtagc aatcactttg ctttggacac ctggtgccca cgtccatgct
     6421 ggattagatg catcggcaat atcacttgct ctggcagatt tgattggctg attcgctgag
     6481 tttgaagagg aaggggtcga ggatgccgct tcttgctgtg ctgcttgtgc gaccgcagct
     6541 tgttgagcgg cttgagctgc agctgctgct tgagcggctt gttttgcttg ttcttcagct
     6601 gcttttttcg cttcttctgc ttttttcttt tcctcttctt cttttttctc tttatcagtg
     6661 agcatcttat ctacttgttt caattgttca ttcaactgag cagatagatc tgtatttttc
     6721 actttttcta ctgcttcttt tgccgtcttg tattggtcgc gtgttgctga atccactact
     6781 tttccgtctt taaagactat agctgttttt tctttagctg tttgcaattc agcatattga
     6841 tttttagctt ctgtttgtgc ttgttgaatc aattttccaa aatctgtgtt atccgtttcg
     6901 attgcttgga tctctttatc tgctttcaac ttgatgtcat ctgctagatg atcatcgacg
     6961 atggcaggtg ctatgaacca ctcattcact ttttggatat ctcggatctt agattggata
     7021 tcttggtaag ttgtttctaa ctctgaatat cctttttcat tcttaagact ggataaatca
     7081 ttttttagtt ttgataaatc ttgatgaatc atatctgcac gaataaattg atggtgatca
     7141 tctgtataaa aggcagcaag tttctcatga agttcttcca tttgttgctg tctttttgct
     7201 tctgctgcta cttcggcttc tactttttga tgatggtcat aataagccca gcttcctcca
     7261 ccgagtagaa gtactgctgc taaaacgaga taaggtactt tttttgattt tttcttttgc
     7321 gctttatctg gatctgtttt ttctaagttt gattcttgat gtgttttttc agtatctggc
     7381 acgattgtta ttgatttcgc ttctgcttca aacagcttag gctggttctc ttctttttct
     7441 atttttgctt caagatcagt accctctttg cttacagatt gattttcagc atcggattca
     7501 ctatttattt catcgggctc tgttgacttt ttgtctgtgt tttcgagtat ttcttttttc
     7561 tctgcttccg gcagtttatt cgttttttcc ttttctaatt gtttttcagt aaatacttca
     7621 gttttttctg tgatatccgt ttttacgtct tttattttta ctgtttcttt acctgtggcg
     7681 ttttctctat cagagtcttt ttcagtttct tgttcctttt ctggctctac tttgttttcc
     7741 tgtgcaattt tttcactgat cattttgagc agctctttcg cgtgctcttc ttttgtatct
     7801 tcacgatgtg ctttgatata agcggatagt atcgaatttt cttcctcatt atcgacgatt
     7861 tctttcagtt cagctactct tcttttttgt tttgattcaa gcgtttcttc ttgattcttt
     7921 cctgattctt ccgaagtctc atgaacagtt gctttcttct gttcattttt ctctatatta
     7981 gttacttgat cgtgagcagg tggttcttcg tcttctggtt ctgctttttc gaataattcc
     8041 attacagatt ctagaggtaa atctttcaat tccgaccatt cgattggatc gtttaattct
     8101 gtatcaggaa agactgcatc ttcagttgat ttatcctgca tagctgcatc tgtatttgtt
     8161 tcttcaattg attcttttat ttgctgatct tctgcagtta tttgatgacc gcagtctgga
     8221 cagatagtat tctgctccat ttttttcgat ccgcaatttg gacatttttt catcacttag
     8281 cgatcggctc ctttactagg tttacgctac catttaatct atcatattct atacgtcacg
     8341 ccaatagctt atttttagga tttacttaaa aataatgaat gaatttcctt cctatagaaa
     8401 aaaaagcttg agactgcgta gtctcaag
//
LOCUS       DAZUMK010000112         8388 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000112 DAZUMK010000000
VERSION     DAZUMK010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.114"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            94..2703
                     /gene="clpB"
                     /locus_tag="ACG808_002333"
     CDS             94..2703
                     /gene="clpB"
                     /locus_tag="ACG808_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFS5047933.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2854..3540)
                     /locus_tag="ACG808_002334"
     CDS             complement(2854..3540)
                     /locus_tag="ACG808_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047934.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(3518..4012)
                     /locus_tag="ACG808_002335"
     CDS             complement(3518..4012)
                     /locus_tag="ACG808_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFS5047935.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(4032..5252)
                     /locus_tag="ACG808_002336"
     CDS             complement(4032..5252)
                     /locus_tag="ACG808_002336"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5047936.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5399..7234
                     /gene="lepA"
                     /locus_tag="ACG808_002337"
     CDS             5399..7234
                     /gene="lepA"
                     /locus_tag="ACG808_002337"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFS5047937.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            7430..8341
                     /locus_tag="ACG808_002338"
     CDS             7430..8341
                     /locus_tag="ACG808_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5047938.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
BASE COUNT         2841 a         1507 c         1693 g         2347 t
ORIGIN      
        1 tattttgacc aaaattgacc aaagcgatat aatagttttt gtaaagatca aagaaataag
       61 tgatcttacc tttaattagg agggtatacc tgtatgaata ttgagaaaat gactacgacg
      121 cttcaagaag cgattgctga agctcaacag attgcggtca ctcgaaaaca tcaagatatc
      181 gacattgccc atgtatggaa aatcttttta caatctaatc attttggtcg aaatttctat
      241 acggatgccg gattggatgt cgaatctttt gaacatgaaa ttgatcgtct actggatgag
      301 tatccagttg tttctggtgg aaacgtccaa tatggacaaa acctcagcca gaatttcttt
      361 cgattattga atgaagcaga ccaaatacgt gaaagcttcg gcgacgagtt tctttcaacg
      421 gaagtcgtta ttttagcgtt gatgaaatta aaaaattatc cgttaactgt ttatttgaat
      481 aaaaacggat tatctgaaaa agaattgcgt aaaaacatcg aagagatgag aggaggagaa
      541 agagtgacct cacaaaacca agaagaacaa tacaaagcat tagaaaaata cggcgttgat
      601 ttagtacagc aagtcaaaag cggaaaaatg gatccaatca tcggtcgtga tgaagaaatc
      661 cgtgatgtca tccgtattct ttcacgaaaa acaaaaaata atcctgttct gataggagaa
      721 cccggtgtag ggaaaacggc aatcgttgaa gggcttgctc aacgtatcgt acgcaaagat
      781 gtgcctgaaa atttaaaaga taaaacgatt ttttcattag atatgggtgc attgatcgct
      841 ggagcaaaat tccgtggtga atttgaagaa agactaaaag cagtcttgaa agaagtcaaa
      901 aaaagtgatg gacgtatcct cttgttcatc gatgaaatcc acaatatcgt aggggcagga
      961 aaaacagaag gaagtatgga tgccggaaat ctgttgaaac caatgcttgc tcgcggtgaa
     1021 ttacatttga tcggtgccac aactttagat gagtaccgtc aatatatgga aaaagacaaa
     1081 gcgttggaac gtcgctttca aaaggtatta gtcaaagaac caacagtgga agatacgatc
     1141 agtattctgc gtggattaaa agaacgtttt gagatccacc atggtgttaa catccatgac
     1201 aacgcattag tagcagcagc tactttatct gaccgttata ttactgatcg gttcttaccg
     1261 gacaaagcaa tcgatttgat cgatgaagcc agtgcaacga ttcgggtaga aatgaactct
     1321 atgccaacgg aattagatca agttactaga cgtttgatgc agttggaaat cgaagaagct
     1381 gcactgaaaa aagaatcaga tgatgcaagt aaaaaacgct tgaaaaattt gcaagaagaa
     1441 ttagctgaac ttcgtgaaga agcaaatgcg atgaagatgc agtgggaaac agaaaaagaa
     1501 gaagtcaatt ctgtttcagc aaaacgtgct gaaatcgata aagcaaaaca cgaattagaa
     1561 gatgcagaaa ataactatga tttagaacgc gctgctgtat tgcgtcatgg tacgatacct
     1621 caattagaaa aagaattgaa ggaattagaa gctaaagcaa aagacagcga gattaagatg
     1681 gttcaagagt ccgttaccga aaatgagatt gctcaagtcg ttggcagatt aacgggtatc
     1741 cccgtaacga aacttgtaga aggtgaacga gaaaaactaa ttaaattgaa tgaaacactt
     1801 cataaacgag tgattggtca agatgaagca gttgatgcgg tcagtgacgc ggtaattcgt
     1861 tcacgggctg gattgcaaga cccaaatcgt cctctaggtt ctttcctttt cctaggacca
     1921 actggtgtcg gaaaaaccga attagcgaaa gcgttagctg agaacttgtt tgactcagaa
     1981 gatcatatgg tacggatcga tatgagtgaa tatatggaaa aacatgctgt gtctcgtcta
     2041 gtcggagctc ctccaggcta tgtaggttac gaagaaggtg gtcaattaac ggaagctgtc
     2101 agacgaaatc cttatacaat cgtcttgttg gatgaaatcg aaaaagcgca tcccgatgtc
     2161 ttcaatatct tgttacaagt cctagatgac ggacgcttga ctgattcaaa agggcgagta
     2221 gtagatttca aaaatacggt tctgatcatg accagcaata tcggttcaca gttactgctg
     2281 gaaggcgtga ctgctgatgg aacgattcca gaagcagtgg ctgaacaagt caacacgcta
     2341 ttacgtggaa acttcaaacc agaattcttg aaccgaatcg acgacacgat tttatttaca
     2401 ccattgagcc tagacaacgt gaaaggaatc gttgacaaaa tggtggcgca attggcacaa
     2461 cgtctggaac atcaagaaat cctgctgaca atcagtgatg aagccaaaac gtggattgct
     2521 gaaaatgctt atgaaccagc atatggtgca agaccattga aacgatttat cactaaggaa
     2581 gtagaaacac cactagctaa agaaatcgtt gcaggacatg ttatgcctaa atcaaaagta
     2641 acgattactt tacttgatgg acaacttcat ttcaaaacag aagagctaga agaaatcgtc
     2701 taatcttcca ttgagactat aaaagaaaaa aggggtctga cacaactagt gtcagacctc
     2761 cttttttcaa ataaacagtg ttccagaagt agtttcttcg aaaaataagc cgaaatttct
     2821 tgaagctgcc cgcttctgtc acaacctctt ttattaggta tttttcactt tttctaattt
     2881 ataactttca aagtaacgct catctttttt actttgagac catgcaaaca atcccattaa
     2941 ggcagcacct atcgtatttg tcagcaagtc gcccatcgta tccatcaatg ctgctcgtcc
     3001 gacaaacagt gtgccatcag aagcagcaaa acgttgaaga ttcatgccta agaactggtc
     3061 gcataagaat tcccagaact cccaaaacac gccgcaaagt ccagcaaacg caaagccgaa
     3121 taagaggaac aaccaaggac tggtttttga tatctcagca tctttcatca agtaaccaat
     3181 caatccatag ccaagagctg tcaggaccat tggactcacc gcatgtaaga ttttgtccca
     3241 aaaagagatg atactgatca aatgcatccc tgtgcctaaa aagacagaga tgaacaagaa
     3301 aaaccagtaa aacaaaacga ttgctgctgg tattttaagt ttgaaaactt tcaaaatgac
     3361 ttgtggcatg aaaatcaatg caatccctaa taaagattcc acgataaatg tgattccttg
     3421 agaagtcgaa tatttttgtg tgataaattc ataaaaatta tagcagacaa cgaatacacc
     3481 aaaagcaagt aaagctattt gcaccttttt gatttcttta atcactgaaa gtccctccat
     3541 ctaaaaatct ttttacttct gaaagcaact caatttctga agaaaccact tttccgttca
     3601 atttgatcaa acctattgta tataagttca ggtagtgaaa ttgattttca gctgtttctt
     3661 gtagtgcagc gatttttttc gggtgggttc cgccttgctg tcgagtatca gtatataagc
     3721 cgatcactgg gatgcctttc gcataagcca caccgatttc cgatgctacc ccggcatcga
     3781 tcgtcagccc atctagtaaa gcaatcatta aatcactagc caacactttt tctgtgtcag
     3841 caagagcaat cattcgactg tctgcataag cagttttgtc attgattgct tcattttctt
     3901 gaggaagata gatcgaaagc tcagaagaga gctctctgat ttctttaact aactgttggt
     3961 tatataacaa atcactttta gcaaacattg gtgcggcaaa ataaattttc atctaaacat
     4021 catccttttt atcaaaaata atcgctaaag taagcttcca tgtttggaat gatacgctct
     4081 agtgacttca gtgtttcttc atctgtttct aaccgttcga ttcttgccaa ataagaagca
     4141 cggcgataat tcatcatcaa gcaagtcaat gtttggatat ccagtcgaac ggattttcct
     4201 ataggttcat ttaagacacg gacttgtccc tgttcatccc atgttaaagc gaagacacca
     4261 ttattccaag gagcgacagg atcttcaatg atgaaatgaa aagcatcagc tgttccaaca
     4321 aacggaaacc gttgaaggaa ttcttttaca tcaacgatcc gagccataaa atatggttcg
     4381 atctgctctt tgatctgact atcttcaatc aaaaatgcta aaggctcatt tttataaata
     4441 tctccatgta cccagtagat catggaaaaa tgtgcggaaa taaagttcca taagccattt
     4501 cgagcttctt gatcaagata gaacatttct tttacgtgaa atatttcttc agctacccag
     4561 tagaacagaa cacctcttgg ttcatggttg ctatcgtagt agacagcggc tgtgcgctca
     4621 tcttcatttt cgaaacgcca atactcttcc caatgaaaat cactgcggaa taaagcacca
     4681 tggttttgtt gggcaaattt gtcataaacg gaaaatacgt ctggatgatc aactggtaat
     4741 cgctcaacca taccacttac tgggatttgt ttaggtagct gtgtatctct gattttaaag
     4801 gaaagtttat cagacatgat ttcccatcct ttacgccgat agtaggggat actgtaagga
     4861 tacaaataag agatccattg cttattcttg cgcatattat ccaatgcaac gataattaga
     4921 tcttgcatca atccatggtt agcatattct ggataagttc caactccagt cacaccaccc
     4981 attttataac gtgcaccgtg aatattgact tcacaaggat agatagcgat ttgagagatc
     5041 agtttatttt cgtggaacca tccgaacact ttcgatactt ccaaaatcgg ttttttggaa
     5101 cggataaaag ctcgtttatt ctcaaacccg ctttcctcga tgtctgcttc agtcacttgg
     5161 aacacatagg aaagcaattc attgaattga tcgatatgtt cttgagtgac cggttttaat
     5221 tcaagatttt ctctaaaatc tttttgatcc attttttcat ctccatttat tagcttgata
     5281 acagtatagc aaaattctac aaaaaaaact tctatagctg actaattaaa aactctttaa
     5341 attactgctt ttcgttggta taatggaaag tgctgattat atgaaaagag gagacgctat
     5401 ggatataaag aaaatgaaag aacgccaaga gcagattcgt aatttttcga tcatcgctca
     5461 tatcgaccat gggaaatcta cattggccga tcgtatttta gaaatgacgc acactgtgac
     5521 gtctcgcgaa atgcaagatc agctgttaga ctcgatggat ttggaacgtg aacgtggaat
     5581 cacgattaaa ttaaatgctg tagaactcaa ttacacagcg aaaaatggag aaacatatat
     5641 ctttcacttg attgacaccc cagggcacgt cgacttcacc tatgaagtct ctcgaagcct
     5701 tgctgcctgt gagggtgccg ttttagtggt cgatgcagca caaggaatcg aagcacaaac
     5761 attggccaat gtttatttag cgttagacaa tgatttggag atcctgccag tcatcaataa
     5821 aatcgaccta cccgcagcag atcccgaacg tgtccgtcaa gaaatcgaag acgtaatcgg
     5881 aatcgatgct agtgaagctg tattagccag tgccaaatca gggattggga ttgaagatat
     5941 tctcgagcag atcgtggaat atgttcctgc accaagtgga gacttagatg caccgctaaa
     6001 agctttgatc tttgactcta tttatgatag ttaccgcggt gttgttctaa atgtccggat
     6061 cacggacggt atggtcaaac ccggagacaa aatcaaactg atgagtaatg gcaaaacatt
     6121 tgatgttaca gaggtcgggg tcttctcgcc aaaagcagtt gctagagact ttttgatggt
     6181 cggtgatgtc ggttatatca cagcaagtat caaaactgtc caagatacac gagtagggga
     6241 tacagtaacg ttagcagaca atcctgccgc tgaagcttta ccaggttacc gaaaaatgaa
     6301 tccgatggtt tattgtggtc tttatccaat tgatacttct cgttacaacg atttgcggga
     6361 agctttagaa aaactgcaat tgaatgatgc tgctttacaa ttcgaaccag aaacctctca
     6421 agcattaggt tttgggttcc gctgtggatt cctcggactt cttcatatgg atgtcgtcca
     6481 agaacgcttg gaacgggaat tcaatctaga attgatcacg actgcaccat ccgttattta
     6541 ccatgttaat aaaacggatg gtacgactgc tacagtagat aacccggcag attttcctga
     6601 acctgtaacg attcaggatg tagaagaacc attcgtcaaa gcacagatca tggtcccaaa
     6661 tgattttgtc ggtgcagtaa tggaactttc tcaacgaaaa cggggagaat tcattacgat
     6721 ggattattta gatgattacc gagtaaatgt cgtctataat attccgcttt ctgaaattgt
     6781 ctttgacttt ttcgacaaat tgaaatcaag cacgaaaggc tatgcctcat tggattatga
     6841 aatgtctggt tatcaaaaaa gcaagttagt gaagatggac atcttgctga atggagaaaa
     6901 agtcgatgca ttaagcttta tcgttcaccg agactttgcc tatgaacgtg gaaaagcaat
     6961 cgttgaaaaa ctgaaaaaac taatcccacg ccaacaattt gaggtgccaa tccaagcagc
     7021 tattggacaa aaaatcgttg cccgttcaga catcaaagcc ttgcgtaaaa acgtcttggc
     7081 taaatgttat ggtggagatg tttctcgtaa acgtaaactc ttagagaaac aaaaagaagg
     7141 gaagaagcgg atgaaacaaa tcggctccgt cgaagttccg caagaagcct tcatggccgt
     7201 tctgaaaatg gatgagcaag atagtgtgaa ataacttata atcaatcgaa aaacctatta
     7261 aataaacctt tttgagtaga tgcaacactg aggattatct gtataaaaga ttttaaactg
     7321 tcatttggtt atcacggcga tactaaaatt aacaaaattg gaatattttt cttgaagatt
     7381 tttagctgtc agtatatgat gaaagcatct cataggaggt gcttttttca tggttgataa
     7441 ttcaagtgga agggttctta aatctctaag aaaagagaaa aaactatctc aaaaaaagtt
     7501 agctgattta gcaggtattt cacaaagtac tttagttaaa tatgaaaaag ggtcaagaaa
     7561 aatccctaaa gacgtagata atactctaag caagattctg aatatagaaa ctctaataaa
     7621 agatgaggaa gataagattg agattctcat cggtaaacta attgcataca gagatatgaa
     7681 taagttactt aacaaagagt tagctgataa tataggaatt agtgaagtat tactcagtta
     7741 tgtgttaaat agaaagagaa atccgtcaaa agaaatgcaa aaaaaaatcg atatttttct
     7801 attaagtaat gaaaaagaaa ttttgaagga gattaatcgt gatagtgaga tatttagttt
     7861 atcaaaagac gataaaattg ttatgggaaa aagaatacga gaagtccgaa agaaccggga
     7921 agagacgctt gaaaaatttg gtaaaaactt taccatctat acaggcaaaa atgttataag
     7981 tagatgggaa aaaggtatta atattccaga tattgaaaaa ctaatgaata ttgcctatct
     8041 tggtaaggtc actgttcctt accttatgta cggagaagat tataagaata tacttccaaa
     8101 agatgaaaga gttagcgatt ttaaaaagat aaattctttt agtatgggat taaggatgag
     8161 gaaaattagg aaagattatt atctagaaag ggaggagttt ggaaagcttt tttctccttc
     8221 aatttcaaaa tggtccatag ataggtatga gaatggtaga gatataccca atactaatcg
     8281 aattatccaa tatgcttata taggaaatct ttctttagaa tttttgattt acgggatata
     8341 aaaatagttt acgtcgatac ttattaagtt atcgacgttt tttattat
//
LOCUS       DAZUMK010000113         8294 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000113 DAZUMK010000000
VERSION     DAZUMK010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.115"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            61..534
                     /locus_tag="ACG808_002339"
     CDS             61..534
                     /locus_tag="ACG808_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFS5047939.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            543..770
                     /locus_tag="ACG808_002340"
     CDS             543..770
                     /locus_tag="ACG808_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5047940.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            1406..1777
                     /locus_tag="ACG808_002341"
     CDS             1406..1777
                     /locus_tag="ACG808_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047941.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2033..2275)
                     /locus_tag="ACG808_002342"
     CDS             complement(2033..2275)
                     /locus_tag="ACG808_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS5047942.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(2307..3209)
                     /locus_tag="ACG808_002343"
     CDS             complement(2307..3209)
                     /locus_tag="ACG808_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS5047943.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(3222..3410)
                     /locus_tag="ACG808_002344"
     CDS             complement(3222..3410)
                     /locus_tag="ACG808_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFS5047944.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(3424..3987)
                     /gene="amaP"
                     /locus_tag="ACG808_002345"
     CDS             complement(3424..3987)
                     /gene="amaP"
                     /locus_tag="ACG808_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFS5047945.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4025..4918)
                     /locus_tag="ACG808_002346"
     CDS             complement(4025..4918)
                     /locus_tag="ACG808_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS5047946.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(4996..5919)
                     /locus_tag="ACG808_002347"
     CDS             complement(4996..5919)
                     /locus_tag="ACG808_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFS5047947.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(5968..6318)
                     /locus_tag="ACG808_002348"
     CDS             complement(5968..6318)
                     /locus_tag="ACG808_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFS5047948.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(6351..7253)
                     /locus_tag="ACG808_002349"
     CDS             complement(6351..7253)
                     /locus_tag="ACG808_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFS5047949.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(7246..8103)
                     /locus_tag="ACG808_002350"
     CDS             complement(7246..8103)
                     /locus_tag="ACG808_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFS5047950.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
BASE COUNT         2280 a         1595 c         1402 g         3017 t
ORIGIN      
        1 gttgttttac aataattatt ctgctatatt ttagctatca aagataaaag agggataaaa
       61 atgaaattta aaacattgct attaaaattt gtcgttttag gtataagtgc cgtatttatt
      121 ttgttcggcg tactttttta tactcagatc gctacaagcg aaaaaggatt caattttgat
      181 tggcagacca cattatttgt ttctgtcatt tactttgccc ttctcctagg gatcgttatt
      241 gcttatttct tattgaaact attgaaaaaa atcgaagata accaagcttt ttccactcaa
      301 agcttaaaca tactaaaaaa aatcgaacga accattttgt ttattggttt cgcctttttt
      361 ggcttacttc ctaaatttat gaaagtagcg gatttagacg atgctccagg tttgatgttg
      421 gtaggaatcg ctgtcgtatt cattcctttt gcagtataca cattaacaag cgtgctggaa
      481 aaactgcttg aacatgcgat tttacttaag acagatcatg atttgacagt gtgaggtgcg
      541 acatggcaat tatcgttaat ttagatgtag tattagctca aaaaaaaatg agtgtgactg
      601 aattgagtga gaaagtgggc attacgatgg caaacatctc tattctaaaa aatggaaaag
      661 caaaagccat ccgtttttcc actctcgaaa aaatctgcca agttttggag tgtcaaccag
      721 gtgatttgtt gacctataaa aatgactcga atactatacc agaagaataa aataaagtag
      781 aacaaacagt aaataaaaaa taatctgaat gatagatgtg tgagactttt agaagttatt
      841 tacgtctatt attcagattt tttatttcga ttgaataccg ttttatctat aaataagata
      901 tgtactgact catacaaagg gggccttttc ttctccatgt actatcactt ttcctgatac
      961 aactggtttc ctgttacgtt gtagttggat ctgcttttag tataatactt tattcattcc
     1021 accagactga tagcagtttg agcagtctaa ataatcatgt agtattacag gccattctgt
     1081 ttcatacctt gaagtgctta aaacttcagc taatggccca ttgtgttaac tcttactcca
     1141 ttctttttta gtaagctccc atatatactg taaagccttc tatatattat gtcatgttgc
     1201 tttttaacat ttactatgta gttttgataa aatgatttga ataacagtga tggacaattc
     1261 ttttttattt ttaacattat tttgataata atgattattt atttaaaaat acattagata
     1321 acatttgtta cgacacgaaa aagcttcaca aacaaatatc gttattttaa aatgagggcg
     1381 aactcacaca attaggagga atagtatgcc atttattgtt tacttttttc tttcattact
     1441 cgtgatctat attccattac caacattttt cctgctcgtc aatcgtctag cagagcaaca
     1501 tgatacgatt gctgctctta cgaaactttg tttacttcta tttgtttgtc tagattactt
     1561 tacgagctat tattggctaa aacagacaag agtaaaaaag agatattatt tcttccttgt
     1621 ctttttcaat ctctttgagt ttctgatcca ttgcttttta cttcatcagt atcatacgat
     1681 ccatttgtgg atcatctggg ttttcaaagt ggtcgtatta ttttgtatgt tcacctttcc
     1741 tatctcaaga aaatttaggg aggcattgtt ttattaaaga agcttctctg tatcaataaa
     1801 gacacattta aggaaaaact tttaaaaatc taattgggaa tttgcccgca taaataagag
     1861 gctgtgacaa aagtcttgtc acagcctctt attccgtata aacagtgttc aaaagcttgc
     1921 atcttcgata atatgcccaa ccaaatgaaa aatatgagaa gctatttttg tttggctgtt
     1981 cttattactt gatgaagaac agctttttca cgacctcttt ttaaaagtct ttttacagtt
     2041 ttcgagcaaa gaatgatacg attagtacta aaatgatggc accaatcaat gatggcacga
     2101 ttgccattcc tgcaatactt ggtccccaag aacctaataa tgcttgacca agtgctgatc
     2161 caactaaacc tgctacgaca tttgcaataa tccccatcga agaaccttta ttcgtgattg
     2221 ctcctgcaat tgctccgata attcctccga cgataagtga ccaaataaat cccatgtttc
     2281 gttcctccta atgttttact tccttattac tgaacccgtg gttctgtatt ttcttttact
     2341 ttttgtgcgc cgcttccagc tgctgatttt actttatctg tttgattcga agcaaattca
     2401 ccggtttttt tcgctgcgtc agttactttg tcttgcaccg tttcttgatc tttttcaaat
     2461 tcttcttttg ttttgatgtc cactacgtta gcgttgactt cgatcacttc taaatgtgtc
     2521 atatttttta cttctttgcc gattacttct tgcatttctt caaagatttt ttcgatatct
     2581 ttgccatatt ccacaatgac atccatatca actgctactt gttttttacc gacttctgtt
     2641 tcgatcccag ccgttacatt atctgtattc acaagttttc ctgccacatt agagaagaaa
     2701 ccaccgtttg cagataataa accgtctact tgttcaattg cgattccgat gattttttgc
     2761 acaactttat cttcataatt cagctcaccg cgcattgaat cttcctgttc agaatcatgc
     2821 tttccttggt tatttgtatg atcagaacca agttttcctt gtccgtttgt gtggtcagaa
     2881 ctaagttttt cttgtgcttt cgcatggtca gaaggatgtt tttgctcttc cacaggttta
     2941 gaaggatcga ttgcatcatg ttgttctgga actttagatt ctggagttcc tggatgatga
     3001 ggtgcatgct tttctgcatc atgtttcttc tcttctactc cttgttgaac gttagggact
     3061 actggatctg gatgtgcaga tactggtgct gttttgttct tttcatcgtt cacaccattt
     3121 gattggtttg gtgtcgttgg gtctggatgt gcagacactg gtttttggtt gttcatttta
     3181 ttgttattat ttttgttatt gttattcatg gatgtttcct cctaagattt aaaatagtga
     3241 tctaagattt tcgtttgttt cagataaaaa ccgatagcga cgccgatcgc tgccatgatc
     3301 aagatcaata aggtcttgaa aaagcccacg gtgatccata aaacggctaa tatcaatcca
     3361 agtaccccaa agatgatagg gagcttgtat gtcgaaaata gttctttcat ttttggtccc
     3421 cccctattct actcgtttgc ggtcattgct tcgtttagct ggttcttcaa agcctgtgta
     3481 tttcaccgat actttcactg gctcttttgt tcccaatatg tgttgtactt cattttcgat
     3541 ttctttcatc aatgttcctg ttttaccaat gagtgctgaa gttcgtttca actccccttt
     3601 tacattgacg gcgatcttgt ttttcgttgc tttgacattt acttttggtg aatggatgaa
     3661 ttcatcttca tgcaagtgag aacgaaccat tccttcaatt gcttttttat ccaacgtcaa
     3721 ctccccatca ccatgtttca gtagaaagtt tccgattgtt tttggataaa acaggaaaac
     3781 aagtaacgtg ataattgtca aaatagcaaa tgcaacggca agccaaaata catattgttg
     3841 cacgtatgaa ttagtgatgg aaaagaatcg aaacttctct agctgaaaag gcaatggata
     3901 ggttgcactt aatagtatcg caaacaaaag cacagatagc aaaagtaatg ttccgatcac
     3961 tccgatcgtt tttacacctt tattcatttt cgcacctcct cataggggtg aaacgatcaa
     4021 cttcttataa gttgatcgct tctccaccag ttactccgta aatttgggct gtaacataac
     4081 ttgctttatt cgatgctaag aaaacataaa ctggtgctaa ttctcctggc tgacctgcac
     4141 gttctagtaa actttgctga ccaaattcag ggatcgaatc agaaggttgt ccttcgtcta
     4201 gttgtaatgg tgtccagatt ggaccaggtg ctacaccatt tacacggatt cctttaggtg
     4261 caagctgttt tgctaaaccg atcgtaaagt tagcgattga agcttttgtt gctgcgtaat
     4321 ctaataaatg ttcgcttgga ttgaatgctt gaacagaagt tgttgttacg attgctcctc
     4381 ctgctgggat atgcggaaca gcttctttca ctaaagcaaa catagcgatg atgtttatca
     4441 taaatgtgtc tttgacttgc tcaagtggta actcagcaat agactgacaa gaaatttgct
     4501 gtcctgcgtt tagtaccatc gtatctagcc cgccaagttc actcacggct ttatctatga
     4561 tttctttagg tgcttgttcg tcacgaagat caaaaggtag taagacaatc ttacgtcctg
     4621 caactttcac gtattttgct acttcctctg catctttttc ttctcctggg aagaattgaa
     4681 gagcgatatc tgccccttct ctagcaaatg caatcgctgc tgcacgaccg atccctgaat
     4741 ctccaccagt gattagtaca cggcggtttt ctagttgatt attgccttca taggattctt
     4801 ctccacaatc aggaacagga atcatattct gctgtaaagc tggtgtatcc tgatcttgtt
     4861 taggaaattt ctccgtatgg tacaagtttc gcgggtcgat taattttggt tctgtcattg
     4921 ctgtattcct cctagttttt aaattgtatt gccaaaacaa atttctttgt ttgatacctg
     4981 ttttaaatag attctttaat ttgtataatc tttccggtat aaatggtaac ctaaaactac
     5041 tgctgctatc aaagagccaa agactacgag tataaagcct agagcagaac ttttacccgg
     5101 taatcctcct gtattcattc cccaaatccc tgcgaacaac gccggaacgg aaatgattaa
     5161 tgcagctgaa tctagatatt tcatgatatg atttagatta ttgtccatca tcccgttgaa
     5221 tagatcacca atactctcta ataaatctcg atagatctcg atcatttttt ctgtttgtcg
     5281 ctgtctcaaa tggacatcat agagtagctg ttcatcgtct aaacgtttga tgaaagtttc
     5341 agaattccat aattgatcca ttgtttcccg ttgatctctt aaggtatgat cgatatatac
     5401 gatgtctctt tcaagatctg cctgtttata cagttcttca ttttccgttg ttttacgagc
     5461 tgctttatcc aaagcgtcaa ttcgcttttt catctctctt agttctttca cgtaatgggt
     5521 gtaaatatct aataatgtat acgtgatgat cttttcaaac gagtttaaat gatttccatg
     5581 ctttgtcagc agccgatcca caaattcggt ttttctatcg atacaagtaa tcaagagatt
     5641 atcggaaagt acaaaagata ttggcgttaa ttttttttcg atacgattat ctgactgatc
     5701 tagattaatc actaccaaag aaaatggatg ctctagtttc gtctcataaa ggcgctccat
     5761 ccgagaaact tcttccggat attcggttcc tacgaaaata tcatcaggaa gctcgaattc
     5821 ttcgattgct tgctcaatct cttcgggctt tgcactatca agtacgagcc aattatattt
     5881 tttttcatct gtttctatta cattcttttc ttcatccaca aagtagtatc tcaatgtata
     5941 cctccttttt ggactttctc gaaactatta gctgaagaag cagttcttta atttgatatt
     6001 ataatcgagt actgcgattt cattttctaa tgaaaagctg tcaagctgtt tgttgaagtc
     6061 catgttcaat cgtttaccga aataattttg ctctaattta gatgtataga tgtccaataa
     6121 tggatgctcg attttagtcg catattctcc aaattccgaa acaaaaggaa tgatttgaaa
     6181 aaagctgcct tttgcaaaag cacctttgtc ccttagaaat ggatcggaat cattgataag
     6241 cgcgagtgcg atttttgtat tttccccaac cttttcaatt aacgtctcta ctgcattttt
     6301 agacattgta atttccattc ctactccccc tcttcagttg ttgtcttcta ttatttaaca
     6361 actacatctg tttttggttg aacttttgca tattttggat atttagggaa tttagctaca
     6421 caacggaaag ccgtcatccc tgcatatttt tcgtaatatt tcttcgttgg cccttcaaaa
     6481 tccttgctcc acggtttcac atgtcctgaa aaatggataa tgtaaggatt gttgcgagtt
     6541 tcttcgtatt cacgttcacc tgctgctgtt ggatgttttt ttgcttttgc catgatatag
     6601 ccttgtgcat tccaacgcgg atgaagtggt aaccaacggt catgtaagat agcatttaat
     6661 gcatcttggt catggaatcg taatttttct ggattatgtt cgatgaaatc taatactttt
     6721 tgcgtaatat tttcatctaa ccattttttc acattgatca gcatcaaacc tgagttgaaa
     6781 taacgcattg atttagctga aattttcatt ttttccagtc gttgatggaa cccagcgtct
     6841 tcaacagctg ctacgattga atcaccgaag tcaagacgcc ataatttaga gatatcttgt
     6901 aaagcgatca tgtcgcaatc catatataag atacgttcaa cttctgtgcc gcggaacaat
     6961 tccggaatcg caatacgata ataagctgtt tctggaatac gatcgctgat taccacattt
     7021 gtaaagaatt ctttattgat tttcaagaat tccacatctg agttcattcg tgcgtttttc
     7081 actgagaagc gtaaaagctg tttactttca aaatcgatat catcgtcaat cacatagaac
     7141 cgaacaaatt tagacgggtt gcaattatct aatactgaga caaataatgc agctaaatgt
     7201 ggtacgaatt ttgtgttaca acttgcgact actgctattt cttttttatt cattgatctc
     7261 gcctactttc gttagagtac tgtgtgctaa tttttttaag tataaattgg tatacggatg
     7321 atctttcaac gtattccaag gtttatcatg tcctgtaaag tgaacgatcg aaggtttttc
     7381 atttccttct ttatactgac gttcatattt ttcatttggt gctggatgac gttcaaaaat
     7441 cagcgaagtc tgcatgttcc attttggatg aagttgttcc caatcttcat aaagtacagc
     7501 attcaaagca tcttggtcgt gatagatgat tcgatcgcca ttttcactga ggtaatggat
     7561 agttttttct gtaatttctt ttttattcca ttgatcaatg tcaatcacca tcactcctga
     7621 attgaagtac aagtcatcgg aatcgattcc taatctttcc aaagctttca cttggcctgg
     7681 atcgataata gcgccgattg tttttccatt taacggctca tcatatagtt ttacgatatc
     7741 gtctagtact aacacatctg aatctaagta aaggactttc ttataatcct ctttagctaa
     7801 taagtttggt aaagaaattc ttaagtaagc ggtagttgtg atatggtcac tgactaggaa
     7861 gtcttcgtag atatcttttt ccactgtcag gaattggata cttgcattac ttgaatattt
     7921 atttaccgtt tcttccaatc cttctttact atattctgat agaccatcgt caatcacata
     7981 aaattttatg cgtcttgttt tattgcaatt ttctaatgca gttgcgatca tcacgctcaa
     8041 gtaaggagca taattttcgt ctgaagcagt taccacagga actacgccgt atctcttctc
     8101 catcaaaatc actccttttt ctaatctttt tctaacctgt tctacatcta gaatattcct
     8161 catttggttt taaagcaaaa tatatgcttt gttttcattg aatattttaa aattttttta
     8221 tcacaaaacg catcggtaag cgctaactat tcgtttttca tggcttttta ttcaaaggta
     8281 ttgaccaaaa acta
//
LOCUS       DAZUMK010000114         8284 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000114 DAZUMK010000000
VERSION     DAZUMK010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.116"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            453..1316
                     /locus_tag="ACG808_002351"
     CDS             453..1316
                     /locus_tag="ACG808_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS5047951.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1445..3067)
                     /locus_tag="ACG808_002352"
     CDS             complement(1445..3067)
                     /locus_tag="ACG808_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS5047952.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3316..3837
                     /locus_tag="ACG808_002353"
     CDS             3316..3837
                     /locus_tag="ACG808_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFS5047953.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4023..5753)
                     /gene="spxB"
                     /locus_tag="ACG808_002354"
     CDS             complement(4023..5753)
                     /gene="spxB"
                     /locus_tag="ACG808_002354"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFS5047954.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5918..6736
                     /locus_tag="ACG808_002355"
     CDS             5918..6736
                     /locus_tag="ACG808_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFS5047955.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6839..7036)
                     /locus_tag="ACG808_002356"
     CDS             complement(6839..7036)
                     /locus_tag="ACG808_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047956.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7205..7783
                     /locus_tag="ACG808_002357"
     CDS             7205..7783
                     /locus_tag="ACG808_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFS5047957.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7878..8015
                     /locus_tag="ACG808_002358"
     CDS             7878..8015
                     /locus_tag="ACG808_002358"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047958.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2499 a         1480 c         1633 g         2672 t
ORIGIN      
        1 aactcctcgg tcacaagtca caatattcaa caaacatgag aagctgtttt ttgaaattgt
       61 tccttgtgac tcggagctaa acggcaggct cacaacctct tggtaaacgg tgttcaatca
      121 gctgaccctc ggtattagct caaaaaacgg caaaatatga ggaactattt tttccgtttt
      181 tttctctaat actcaggtca aaacgctgat ttcacaacct ctgattcacg gtgttccaga
      241 agtagcttct acggaaataa gccgaaattt cacaaaaatt tgagaagcaa ttttcgtaaa
      301 ttccttctta tttcttgaag ctgaccactt ctgtcacaac ctcttatttt gattagaact
      361 tgcgaaaata aaccggttaa agtgtgtcta gcgcaagaga tttgctataa tagacgataa
      421 gggtaaaatg taacgagaag gggttgaaaa atgtgtatca aattgtaaca gattcttgtt
      481 gtgatctgcc ataccatgta ttagcaaaag aaaaagttga ttttttatca atgaatctcc
      541 aattgaatga tcaagagtta gtagatgacc tcggaaagac attcaattat gaccgctaca
      601 tgaatcagct gaaaaatggt gcgatgccta cgacatcaca aatcaatgtt ggccgatatt
      661 tagaattttt ccgttcttat gcggaaaaac agattcctgt tttatatgtg gctttttcca
      721 gtggattaag cggctcttac agtagtgcgc ttcaagcggt tgaattgctg aaggaagaat
      781 atgaagaccc tgagatctat gtttttgata caaaagctgc aagtattggg gaagggcttt
      841 tagtattgga agctgtaaga ttgaaaaaag aaggtaaaag catcgaagaa accctagagt
      901 ggctggaaag taattatttg aaagtccatt catgggtaac agtagacgat ctgaaatatc
      961 tagaacgagg cggtaggatt tccaaagcaa gtgctactct tggcagtcta ttgagtgtaa
     1021 agcctattat tacggtggat aaagaaggaa ggctgacgaa tgttgacaaa gttcgaggaa
     1081 gaaacaagtc gatccagaca atcgtcaatg agacggtaaa aacaatcgtt gcaccacttg
     1141 atcaaatcgt ttatgtggct tacgctgggg atctagagag cgcggagaag gcaaaacggc
     1201 ttttggaaga gcagataaag atgaaagatg tgaagcttta tcccttagga ccaacgatcg
     1261 ccagtcacac aggctatggc tgtatcgcga ttttttcgat gggaatcagt cgataaaagc
     1321 agtaactcat tctgtaaaaa agcccagaaa ttgattgtaa caaaaacagc gactcttaaa
     1381 agatagatga gcatcctatc tcttagaggt cgctgttttt ttatttttta agctaaatag
     1441 gccattatgc ccataactcg ttcaattgtt tttgaatatc tgcattttcc aaaaattcat
     1501 catatgtcgt atcagctcga tcgatcacac ctttatccga aacggcaatg atgcggtttg
     1561 ccaatgtttg gataaattga tggtcatgtg acgcaaataa gattgagcca gtaaatgcca
     1621 tcaagccgtc attcaatgct gtgattgatt ctaagtctaa atggttcgtt ggatcatcta
     1681 atacaagtac gttagctttc gaaagcatca tttttgaaag catgcaacgg actttctctc
     1741 caccagataa aacattcact ggtttcaata cttcttcacc agaaaacaac attcttccta
     1801 agaagcttct taagaaggtg ttatcatctt cttctttact tgcatattga cgcaaccagt
     1861 ctaaaatcgt tagatcagta tcgaattctt tcgttgtatc ctttggtaaa taagaattac
     1921 ttgttgtcac accccaacgt actgttccag tatctggcgt aatttcaccc atgataactt
     1981 tgaatagcgt tgttgttgtt atatcatttt ctgcgacgaa agcgaccttg tcttctttgc
     2041 tcaaattaaa agatatatca tctaaaattt tctttccatc aatcgttaca cttacatttt
     2101 ctacctgcaa taagtcgttt ccaatctcac gttcaggttt gaatccaacg aatggataac
     2161 gtctagaaga aggttggata tcgtctaaag taattttttc aagcattttt ttacgtgaag
     2221 ttgcttgttt tgattttgat gcattcgcac taaagcgggc aataaattct tggagttctt
     2281 tgatttgttc ttcttttttg gcatttgcgt ttgcttgaag tttagccgct agttgactag
     2341 attccaacca aaaatcataa tttcctacat aaagtttgat tttaccaaaa tctagatccg
     2401 ccatatgtgt acatactttg ttcaaaaagt gacggtcatg ggatacaacg atcacagtat
     2461 tttcaaaatt gatcaaaaat tcttccagcc aattgatcga ttggatatcc agaccgtttg
     2521 ttggttcgtc tagcaaaaga acatcaggtt taccaaatag tgtttgtgct aataacactt
     2581 ttaccttttg tccaccagtt aattcactca tttgctgatg gtgcaattct tcagggatgt
     2641 tcagaccttg cagtaaaaca gctgcttctg gttcagcttc ccatccatta agctcagcaa
     2701 attctccttc taattcagct gctcgaatac catcttcgtc agtgaaatcc tctttcatgt
     2761 aaatcgcatc tttttctttc agcacttcat acagacgctt gtgtcccatg atgactgttt
     2821 caagtacagt atagtcttca tagtcgtaat ggttctgttt caatacggcc atccgttcat
     2881 ctggtcccat agtaacggaa cctgtggaag gctgcagatc ccctgataac actttaagga
     2941 acgttgattt acctgcacca tttgcaccaa tcaagccata gcagtttccg ggggtaaatt
     3001 taatattcac atcatcaaat agtttgcggt ctgaaaagtg caaactcaca tcatttacag
     3061 taatcaattt ttttcctcac ttttatcatt attagttttg acaagtctta tagaattata
     3121 taggggaaag gtaaaagatt caaggcaatc gaacattctt tgtgtttcac gtgaaacaga
     3181 atgaaaaaaa taaaaataaa agcggagcga aaatagctac tataggattt attagtttgc
     3241 aacagcataa tttttgacgt tatttataag agggagtact ataaaactgt aaaagaaatg
     3301 aaagaaggat tgaaaatgga tgaaaaaaga accatcgact ggggctcatt gattttggga
     3361 attttatttg tacttgtctc attgttatca tttcaagacc ctgtcggaaa tttagttgca
     3421 attgtagtag tctttgcgat ttttgccttt attaaaggga tatttgaact gtttgtacgg
     3481 aacagattaa aagagctaac aggatataaa ggaaagatgc ccttaataat cgggatcatc
     3541 gatattctag ttggcgtctt tttcttattc aatattggtg caggagtcgc agcattgcct
     3601 tttgtttttg cagtatggtt tattgctgat tcagttttag cgttatttac tgctgacttg
     3661 gcaagaggag taagtgaagg atattattgg tttacgatca tcgtcaatat tttaggaatt
     3721 ttattaggaa ttttcttatt gtttaatcca atttcttctg cgttgacatt aagcttttta
     3781 gttggattct attttatgtt atttggtatc acacatatcg tctatgcatt tagataagta
     3841 aaatcaaaca aataaaaaag ccggcttccg attacagttt tactgttgta attggaagcc
     3901 ggctttttta tcatggacaa atatgcacga ggtgattttt aggtgtcgag acaaatttat
     3961 gcaaaaatgt gagaagctat atacacaaat tttatcttaa tactcgaagc atagcgcagt
     4021 tgtcacaacc tcttttcata taattcattc aatactggca aatcatggac ttcatatttt
     4081 tctttgaatg cttggatttc ttcttcagaa tattttcttg gatctaaatg cagatcttct
     4141 actggcaatg ggcgcttgtt attgatcttc acatcgatta ctacaggacg atctgatttt
     4201 gcagctgcat caaaggcaaa tcgaagttgc gagtattctg tgactgtaaa gctatttgcc
     4261 ccgagcgctt ctccaacttt accgaaatct gcatcttcta aaaatacacc aaatttcttt
     4321 tgttccgtat cttcttgttc tgcttcgatg aaaccaaaag aatcattcga aaagacaaca
     4381 ttaataattg gaagctgata tttgacttgt gtaatgatat cttgcatgac catagcaaac
     4441 ccgccatcac cacttaaggt aaatacttgt ttttctgggt agcttagttg tgcggcaatt
     4501 cctcctggca caccattacc catagttgcg aaccatccag atgtcgtatg tttttgttta
     4561 ccgttcatat tcaataaacg aatagaatgg atagtcgtgt taccgacatc tgtgacgaaa
     4621 atcgcatcgt cttcagcaat acgattgatt tctttataga cagcctctgg acgaatcggt
     4681 tcttcctctc gatcttcaaa agatttcaac caatttaccc atttttcttt attttcttga
     4741 tttgctaggt accagctatc agcagggcga gcatccccta actcgacaag tctttgcaaa
     4801 gctgtgtttg catcaccgag gatcgacaaa gatgtttcat ggcgtcgtcc aaattttgac
     4861 gcatcgatat cgatttggat aaattcagca gttggattaa aaaatgcacg tccaaatggg
     4921 aagtcgctac cgacaaataa gatcagatcg gcttctgcca atgcttcatt tccgggttta
     4981 gtagcgacac gtccagcaaa accaagaaag ttttcataat catcaggaat aattcctttt
     5041 gctagaacgg aagcgacaac aggaactgaa aaatgttctg agaattcttt gatttgcgga
     5101 aatccgtttc gcgttccttg accaacataa agaactggtt tttttgcttt ttcaagataa
     5161 ggcaaagcag ctagcaaatc tttttcttct ggtaatatca cacttgtttt gtgagtgtga
     5221 gcggtggaga aaggttgttc gtcaatttct tcaaaaccaa gatcaacagg gatagtcacg
     5281 atagccacgc ctttatgctc ataggcggct ttaatggctt catctactac atgcggaaga
     5341 ctttgtggtg tcatgactgt acgattgtat acgctaacat ctgcgaaaat aggattttca
     5401 tttagttcct ggaaagagtt atagttcatg gatgtagaag caacttgtcc taagaacgct
     5461 actagaggca catggtccat ctgtgcatca tacaaaccat tgatcaaatg tgtcgcacca
     5521 ggtcctgcag agccgaagac agcgccgact ttccccgtta attttgcatc tgcagcggct
     5581 gcgagtgcgc cgacttcttc atgacgaact tgaatgtagt tgatttcggt tttttcttga
     5641 tatagcgcat ccataattga attgaaagat cctccgggaa tgccgtaaat atggtcgata
     5701 ccccagcttt caagaacttt taccattgcg attcctgcat tgatttttga catggtgttt
     5761 cactccttta gcaatgatga tacataaatt ttttgtatct agagtataga actttttatc
     5821 gaaaatccca actaattcgt cttttcacaa aaatgtagtc atgtttttgg gaatatgcta
     5881 gagtagtaca tagatagatt caagagaggg gaatggcatg gttgaatggc tattgagact
     5941 tgaaccttgg cagcaagcac ttgctgggac agcttttact tattttatga cagctcttgg
     6001 tgccgggttg gtttttttct ttaaagaaat caaaaaagaa gtgctaaact tgatgttagg
     6061 atttgcttct ggtgtcatga ttgctgcaag tttttggtct ttgttagatc cggctattac
     6121 aaaagcagaa gaaaatggag atattgcttg gctagttgta agtattgggt ttggtttagg
     6181 cggtgtgttt ttatatatgg cagataaaac actgccccat atgcattttg gaccacaaca
     6241 tgaagcagaa ggattgccta cccatttaaa acggacgatc ttgcttgttt tttctattac
     6301 attgcataac attcctgaag gtttagccgt gggggtagca ttcggtgcag cagcaactgc
     6361 ggataatccg acagccgcta tattagccgc tgtttcggtg gcgctaggta ttggtatcca
     6421 aaacttccca gaaggagcag ctgtatcaat cccgttgcga caagaaggtc tgagtcgtaa
     6481 aaaggccttt gtttacggac aagcttctgg aatcgttgaa ccaattgctg gagtgattgg
     6541 agcgctacta gtaacgaaag tggagctgct gcttccttat gcacttgctt ttgccgcagg
     6601 agcaatgatt tatgtcgtgg tggaagaatt gatccctgaa gcacagcaga cattgtccag
     6661 caaacgtcat tttgcagttt ttggcgtcat gagtggattt atcattatga tgattttgga
     6721 tgtggcatta ggataaataa aaaaaatata tatgatatat caaatagggc cgcgaactta
     6781 tatcgcgacc ctattttttt agtaagttat atcataatga agtgaatggt atcatacatt
     6841 aataatagtt ttttctatgt gtatttcctt tgatccagcg aacaagccag atgataagtg
     6901 cgattgggat tagaactttg accgcaaacc ataaaagccc gcttaggaca ctgacaaaaa
     6961 gactgatgac gaagctagct gcaattagca ctaaaagaac catgaagata tttaagaaca
     7021 tatttttttg acgcatacaa aaccctcctc ttgttcattc tttactatag aaaaagtata
     7081 acagggattg tttaaaaaca cctccaactg aagactgatt tttacttcat ctacttaaat
     7141 gatcaaacaa ttgctttttc tctggtttat catctaaaat agagcggaaa aggaggagaa
     7201 tatcatgcct aaaaatcgtc ttgaagcatt tacagatgct gtaatcgcga ttatcatgac
     7261 tattttagtt ttagaacttc atgaacctaa cggagacacg tgggaggcgc tcgctgcatt
     7321 aggttaccga ttttttatct atattgtcag ttttatcatt ttagcgattt attggaacaa
     7381 tcatcatcat ttatttcaag ttgtacataa gataaatgga cgggtcttat gggcgaacag
     7441 tttctttgtt tttgcgcttt cgctttttcc gtttgcaact tcatggatca gtgagtacat
     7501 caatagcctg gctccagaaa tgacttatgg ttttgttacc ttgaccgcta atattgcgta
     7561 ttatatcttg gcaaaagaac ttgtcaaagc aaataaaggc cacgagaaag cattgaaggc
     7621 gatgttgaat aattacaaga aatcgtatct atcgattggt ttgaatgtgt taggaattgt
     7681 cttagggtat ttagtagcgc cagtagccgt gctgatcgca aacatcgtga ttctagctgc
     7741 ttggatcatc ccaagtcgta ccatcgaatc acaatatcgg taaaatgatc cgtgtttaaa
     7801 atttaagaga aaaatataga aaaaataaat aaacgatcaa atatccgagt gatactagta
     7861 ttcaaagagg gatgcagatg gatcaaaaaa gaattcatgt aggactgatt actagttttg
     7921 tcacgatgct tatttatttg gatcaaggtc atttgacttg gacagtagtt ggtgtcgtag
     7981 gatttttgac attattcaca gcaataaaaa aataaacatc tttatgtaat ctgatgttct
     8041 atctatttaa gaaaagcatg tttacagcag tatgattctg ttgtaaacgt gtttttattt
     8101 gtggaaaatt taatttttag aaaagtgtat cgaaaaatag atagttagtt tttccacagt
     8161 ggataacttc gtaagagata aaaataaaaa attagtcaaa gaagggcgta tagatttctt
     8221 ttgataggag atatacaaca gaaatgaata aatattaacg tgttttcaaa cagtataaaa
     8281 cggt
//
LOCUS       DAZUMK010000115         8155 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000115 DAZUMK010000000
VERSION     DAZUMK010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.117"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            383..571
                     /locus_tag="ACG808_002359"
     CDS             383..571
                     /locus_tag="ACG808_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFS5047959.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            649..1662
                     /locus_tag="ACG808_002360"
     CDS             649..1662
                     /locus_tag="ACG808_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5047960.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYWGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(1729..2763)
                     /locus_tag="ACG808_002361"
     CDS             complement(1729..2763)
                     /locus_tag="ACG808_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5047961.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            2878..5103
                     /locus_tag="ACG808_002362"
     CDS             2878..5103
                     /locus_tag="ACG808_002362"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFS5047962.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            5121..7003
                     /locus_tag="ACG808_002363"
                     /pseudo
     CDS             5121..7003
                     /locus_tag="ACG808_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
     gene            6984..7958
                     /locus_tag="ACG808_002364"
     CDS             6984..7958
                     /locus_tag="ACG808_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFS5047963.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         2646 a         1479 c         1715 g         2315 t
ORIGIN      
        1 agttagtcta cattattttg gcgatttgcc aataaggact gttgagttgt gcaaaatcaa
       61 atctgggtca gaatgaacgt cattctgtct cagatttttc gttatttcaa tttgattaac
      121 tgattgatca aaaagatttt tattttcata ttctcaaatg attcaacact aaatatacat
      181 gtttgacgcg gaaattatta ttcgattttg gcggcatcag gggggatgac gacgtagcag
      241 acccattatt ttactcaaga aaatgtccgt gcctattatt tagaatacga tagtgaacgt
      301 gctgggagtt ttgttctttt agagaaagtc tctgaagata aattagttgt cttagatttt
      361 gactgtaacg aaaaatggcg aaatggaaga gcaccagcaa gtgattgacc ggatctacga
      421 agcgacacga tatctgcctt tggatcgttt gtgcttaagt cctcaatgtg gatttgcttc
      481 aacagaagaa gaaaatgttt tgtttgaaga acaatggaaa aaatttcttt tgtcaaaagc
      541 attgcagaag aagtttgggg aaaatcatta aatcatctag gagagcttga taggaaataa
      601 cactatggta taatcagaat gcttaatact aactactgag ggagccttat gaaaaagaaa
      661 attcttaaat ttatcggatg gttcttcgga atcattcttg ggatcgcgtt tgcaatcttt
      721 cttgccttcc agctttcccc cagacctgga gcattcttga tcaaccatat gttttcaaat
      781 actgttcaaa tcactgataa aaaaacgttc gatcaagcag aaaaaaacgt ggcgaaaaaa
      841 acagatttga cgtatgagtc caagtataaa gagaatactt atgacatcta ctatccaaaa
      901 aacagcaagg ggcctgttcc tgttctattt tgggtacatg gtggaggatt cgtcggcgga
      961 gataaatcgg gtgtcaaaga atttgcgaca aaattagttt cagacgccaa catcgcagtg
     1021 gttgcgatga attatgaatt agcgcctgat tcagaatacc ctaatcaagt tcttcaagtg
     1081 aatgaactga tcaaagattt attagctgaa gaaaaagacg acaaattgct agacatggaa
     1141 caattatttt ttggtggaga tagtgcaggt tcacaaatcg cattgcaata tgcagctata
     1201 caaacgaatg atgcgtatgc taaacaaatg aaaatcaaac agctacttcc aaaagatagt
     1261 ctgaaaggaa ccatttctta ttggggacct gttgatttga agctaacagc acagcagcat
     1321 tcggatgatc gatttatgaa gtttttcgtc aaaaccgttg cgtggtcgtt gattggaaca
     1381 aaagattgga aaacgagtga gcaattgaaa gaagctagtg tggccgacca tgtttctaag
     1441 gaatttccgc caacgtatat aactgacgga aatgcatact cattccagga gcaaggtcaa
     1501 gcattagaaa atcggttgac agaattaaag gtacccgtgc aatccttgtt ttatacagat
     1561 acaaaaaaag agattaccca tgaataccaa tttgattaca cgctaaaaga atcgcaaaat
     1621 tgttaccagc agacattgga ttttgtgaat aagtataagt aagaggttgt gaaaaagccg
     1681 tttcgttttg agtatgaaga aacgaagcgg cttttcacaa cctctctttc aatacctttt
     1741 tgttcttggg ataaaacttt ccctaacaat caatcgtgca ggataagtca aaatgattgg
     1801 ttcgtcccgt ataccgtcaa ttcgctccaa tgccattcga acagctgctt gcccaattga
     1861 tttcgcattg atttgaacac tggtcaagcc aggggagagg tttgcgattg tctcgatatt
     1921 atcaaagctt gctacaacaa tctcttctcc aatttggata tttttttgtt tcaatgcccg
     1981 atagacacca attgccattg gatcgctccc gacaagaatg gcagtaggaa gaggggattc
     2041 attttctaat agattagtcg caagtacctc accttgtttt tctgtccagc cacctagatg
     2101 ataagacata tatttttcta agtgatgatc gatcataaaa tgctgatagg cacgcatcct
     2161 cttttcattt atagaaatct gcttcccacc gaattcatcc atatcgactt tatatccccc
     2221 gatataggct attcttttat gaccattttc taagaacaat tccagtacgg ttttagtcat
     2281 ccgttcaaga tctgcataca ccatatcgac ctcttgctgg atgtcagggt tatccacgac
     2341 gactatttgc atgttttgtt ttaacaaggc ggtaatcgat tctttcgtaa cagtcccaac
     2401 aacgatcacc gcacctaact gatctagatc ttcccattgt ttaggattat cagatagatt
     2461 atagattcga ttcatcccta aatgcagacg tcgagcttct tcttccatcc ctactcgcaa
     2521 atgtttgtaa tagatatcct gttcttcgct cgctttgccg atcgtagaga tcacaccaat
     2581 cgaaggcatt ctctttttga tatatttacg agtactgatg tcataatcca acgcagcagc
     2641 tgcctctact atccgttgcc gagtagtctg agccacatgg atcgtctgat cgttattcaa
     2701 cacacgggaa actgtagcag gagagacatt tgcctttcgt gcgacatctc taattccaac
     2761 catttttaca cactcctcaa aaaaataaaa aatttttatt gacaaaattg tttagtaaac
     2821 agtatacagg aattataaaa aaaagtaaac ggtttccgtt ttgttgggag tgaaatgatg
     2881 gatattacgc atagaaaaac agataaaaat ttcatcttat ttgatgaaaa gagccgagtc
     2941 ttccatttac gaaatacatt tctttcttat ctgataaaga tagaagaaag caatgtattg
     3001 gcgcatattt attttggcaa accagtcaag cagtacaaag acaataaaaa ttatccccga
     3061 agagatcgtg gattttcagg aaatgttccc ttaaatcctg accggtctct gtcgaaagat
     3121 acactaccgc aagaatactc ttctcatggc agcatggact ttcgaacgcc cgcatcgatc
     3181 attcagcgca agaatggatc agacttactg gatctgagat atgacagcca ttatattaca
     3241 gatgggaagc cagagatcga aggcttgcct caaacttatg tgatggacaa gtcagaggca
     3301 caaacgttgg tgatttcact taaggataga gaaacagcga tttatttcga tttgttctat
     3361 accatattta ctgatagagc agtgatcaca cgatctgtaa agataagaaa tgaaaccgga
     3421 gaaacaatca aactagaaaa agcggctagt ttccagctag actttgccca tactaggcga
     3481 tttgatgaag tcattgcttt gccaggagca catgtcaatg aaagacagat ttcacggcaa
     3541 tctgttttat ctggaacaaa agtatttgaa agccgtcgag gaacgagcag tcaccatatg
     3601 aataacttta ttgctttagt tcatcatcat acgacagaaa atactggaga agcgatcgga
     3661 cttcaatttg tttattccgg caaccattct ttcgaacttg aaaaagatca gatcaatcaa
     3721 ttgagagtcg tcggtgggat caacagccat cgtttttcgt gggaattgaa tgcaggccag
     3781 tcttttcaaa cacctgaaat gatcctttct tactcaagtc agggattgaa caaaatgagt
     3841 cagatccacc atgagttgct acgtgaaaga atcgcaagag gtcgtcatca atttgctgaa
     3901 cggccgattc tggtcaataa ctgggaagcc acttactttg acttcaatag tgaaaaaatc
     3961 aaggcgatca ttgatgaagc aaaggagcta ggcatcgaaa tgttcgtatt agatgatggc
     4021 tggtttggaa aaagagatgc ggacaattct tcattaggag actggttcga gtatgaaggc
     4081 aagttgacaa atggattacg tgaaatagca gattatgctc atagcaaagg attgaaattt
     4141 ggtttatggt ttgaacctga aatgatctcg gttgattctg aactgtatcg aacacatcct
     4201 gattttctta tgcaggagcc aggtagaatg ccttctgctt ccagatcaca acatgtactg
     4261 gattttacaa ggttagatgt tcggcagacg attgagaaac aaatgagaaa gattttagat
     4321 acgattcctc ttgactacat caaatgggat atgaaccgtt cgctttcaga tgtctattcg
     4381 attacccttg acccacagag acaaggagaa gtagcgcatc gttatatgct cgggctttat
     4441 gaactacttg aacatttgac aactgattat ccggaaatct tatgggaagg ctgttctggt
     4501 ggaggtggac ggtttgatgc cgggtttatt tattatatgc cgcagtcatg gacaagtgat
     4561 aacacggatg cagtggagcg tatgaaaatc caatatggaa caagccttgc ttatccgatt
     4621 tcgtccatca cagctcatgt atcagctgta ccaaatcatc agaccgacag atctacttcg
     4681 ttgaaaacaa gaggagaaac agctatgagc gctgtttttg gttatgaact ggatttgaca
     4741 aagctgtctc ctgaagaaaa aaagcaagta aaagaccaaa ttatatccta tcaaacgatt
     4801 cgtccggtga tccagtatgg ccattattat cgtttggcga gtccttttga agaaaatatt
     4861 gccgcatgga tgtttgtgtc tcctaaacaa gatgaagcta ttgtgttttt agggaggata
     4921 ctagcttcag ctcaaccagc gttccatgaa gtatatctga tggggttaga tgatgaggca
     4981 ctttatcagg aacagacctc gaagcggata ttttcggggg ccgaattgat gacagttgga
     5041 ctttacttcc ccgattttca aggtgatttc caaacagaac tgcttcattt caaaaagtta
     5101 tgagagagaa ggaaaaaagt atgaaagcaa atataaaatg gttagatgat ccacaagttt
     5161 ttagagtgaa ccaatcaaat gcacatagcg atcatgtttg ttatgcaaca agggaagaag
     5221 tagaaaccac atcctcctta aacagtcttt aaatggatca tggcgttttt gttattcgcc
     5281 aaatccttcg gtacgaccaa aaacatttta taaaaaagat tttgatgcct gttcttttga
     5341 tgaaatcact gttcctagtc atattgaatt agcaaattat gacaagatcc attacatcaa
     5401 tacgatgtat ccgtgggagg gacatgtatt tcgccgtccc cctttcacga caagccctga
     5461 gaaagaggcg gaagggatct tttcagctgc taaagacaat ccggttggtt cgtatttgac
     5521 gacttttcga ttagaagaag gtctaaaggg aaaacaaatc tcgattcatt ttgctggtgt
     5581 agaacaagca atgtttattt ggttgaatgg tcattttatc gggtatgccg aagattcgtt
     5641 cactccgtct gaatttgatt taacacctta tttggaagaa gaaaatattc tagctgtgga
     5701 ggtttacaaa agaagtacag ctgcattctt ggaagatcag gactttttcc gattttttgg
     5761 gatcttcaga gatgtcttct tagttgctcg aggacaaaac catttagaag atctttggat
     5821 caaacctcgc ttagatgaca aatgtcaaaa tggcaaactt gaagtagatt tgtcttttga
     5881 gcaagtagcc aaaaacagtc agctgaatgt agcgatacta gatcctgatg ggaaggaaat
     5941 ctatcaacag caatatccgt taaaagagca aatgacgatt cgcatcgatg gatttgaaaa
     6001 tgtggcactg tgggggcatt ttcagcccaa tttatataag cttgaattgg agacgataga
     6061 cgaagaaaaa agagtaacgg agtatattga ctattccttc ggttttcgta aaatcgaaat
     6121 caaagataaa atcatctact tcaatggaaa acggatgatt atcaacgggg taaatcgaca
     6181 tgagtggcat ccgaaaaaag gacgcgccat cactatagaa gatatggaaa aagatttggc
     6241 tatcataaaa aatgcaaaga tcaacggcgt acggacatct cattacccca accagatcac
     6301 ttggtattat ttatgcgacc aagcaggaat ccatgtgatg gcggaaacga acctcgaatc
     6361 ccatggctca tggcaaaaaa tgggcgcatt tgagccttct tacaacgtac caggtgcagt
     6421 acctcagtgg caggcggctg ttcttgatcg agcaaaaacg aatttcgaaa cattaaaaaa
     6481 ccatccaagt attctttttt ggtctcttgg aaatgaatct tatgcggaag aaaacattcg
     6541 acaaatgaac gattacttca aaaaagtaga ccccgatcga cttgtccatt atgaagggaa
     6601 ttttccaaat ccagcattcg aagattcgat ttcagatgta aaaagctgca tgtatgcgcc
     6661 accagaagag atcattgact accttgaaaa taaagcggaa aaaccgttca tcttatgtga
     6721 atatatgcat gacatgggga attcgttagg tggaatggat tcttacatga cgttgcttga
     6781 tcgttatgaa atgtaccaag gaggctatat atgggatttt aaagatcagg cgatccaagt
     6841 gactgatcca atcacaggca gagaagtgat gcgctatggc ggtgactttg acgataaacc
     6901 aagtgactat gaattctcag ggaatgggat cgtttttgca gatggacaag aaaaacccgc
     6961 catgcaggag gtaagatatt attatgaaaa atacagtaaa taaaagtcat atggatacgg
     7021 aaaaagttgc aatcgtcttc ggcgactgta cattaggtgt caaatcgggg aatacgcatt
     7081 atattttttc ttatacaaga ggcggactgg aatcgctcaa taaaaacgga aaagaatggc
     7141 tataccggga aacacttcca gttttttggc gagcaacgac cgataatgac agaggtagcg
     7201 gatttggatt taaatccggt atgtggttag gagccggatt gtatccaaaa gtcaaagaaa
     7261 tagaagtact ggtggacaat aaaaagatcg atttgccgat tgctccagta aataatcaat
     7321 attcaagtaa tgaatacgct gaaacggttg aaatcatttt tacactggaa atcaacacag
     7381 taccaaaaac ccaagtgaca atttcctacc aaatcgctgg agagggtaaa atgcttgtca
     7441 atatgcatta ttatggacaa aaaggattac cggaattacc cgcattgggt ttaaggttca
     7501 ttatgccaac gaaagcaaaa ttttttgagt atgaaggcct atcaggggaa acatatccgg
     7561 atcggatggc tggtggtgtc gaaggtattt ataaagtggc aggactcccc gtttccccat
     7621 accttgttcc ccaagaatgc ggcatgcata tggaaacgaa atgggtagaa ctaacaagaa
     7681 atcaaacgtt ggataatacg gataaagatc aagaagattt ttcgttgcga tttgaacagc
     7741 aggatacgcc atttgctttt tccgctttac cattcaaagc agaagaactg gaacaggcaa
     7801 cacatataga agaactaccg ccagaaagac gaacggttct tacgattttt gcaaaagtaa
     7861 gaggagtcgg gggtatcaat agctggggag cggatgttga gtcaccttac cgaatcagca
     7921 gcgaagagga ccatgaaatc ggctttatta ttcagtagca atctttagat tattatttga
     7981 aaaacggagt ttacaagaca aagctgcttt attctatatg tcatgaacaa gagagtaaaa
     8041 aagttctcag atacttttgt tcatgactat tttcttcaat aaaaccaatt gttatttttc
     8101 tgaaaaacaa agttaactat cagaaaatta aaaaaagttt cataaaaatg attga
//
LOCUS       DAZUMK010000116         8068 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000116 DAZUMK010000000
VERSION     DAZUMK010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.118"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..884
                     /locus_tag="ACG808_002365"
     CDS             <1..884
                     /locus_tag="ACG808_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFS5047964.1"
                     /translation="ISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTY
                     NDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVL
                     GVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMT
                     LAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILA
                     VVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGW
                     IIGAILCLITFLLYGNA"
     gene            932..3061
                     /locus_tag="ACG808_002366"
     CDS             932..3061
                     /locus_tag="ACG808_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFS5047965.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3036..3725
                     /locus_tag="ACG808_002367"
     CDS             3036..3725
                     /locus_tag="ACG808_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047966.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(4133..4819)
                     /locus_tag="ACG808_002368"
     CDS             complement(4133..4819)
                     /locus_tag="ACG808_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5047967.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(4955..5149)
                     /locus_tag="ACG808_002369"
     CDS             complement(4955..5149)
                     /locus_tag="ACG808_002369"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047968.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5199..5639)
                     /locus_tag="ACG808_002370"
     CDS             complement(5199..5639)
                     /locus_tag="ACG808_002370"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFS5047969.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(5643..6488)
                     /locus_tag="ACG808_002371"
     CDS             complement(5643..6488)
                     /locus_tag="ACG808_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFS5047970.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            6637..8055
                     /gene="pepV"
                     /locus_tag="ACG808_002372"
     CDS             6637..8055
                     /gene="pepV"
                     /locus_tag="ACG808_002372"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFS5047971.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
BASE COUNT         2481 a         1515 c         1714 g         2358 t
ORIGIN      
        1 gtatttcagt accggttgca ctgttctcat ttatgggctc gattatggct ggaatctttg
       61 ctaatatcgt caatttcaaa caatatcaaa cgatttatgc tggtttcaat ccagcggctg
      121 aaagctatac ttataatgac tatttccaaa ttggtatgat tgggatgatt gtaagcttag
      181 ttgtggtact aacggtcgct aatatatcaa tgaacaaaaa gaaacgctat gcaatggcag
      241 caaacgtacc agctgaaggt ggcgacgcac caatgatctc atggttagcc gtattattac
      301 cagttttagg ggtcgttctt ttagatcttc aaatcatctt aggttttatt ctagcaggta
      361 tctgggcatt gctgtttact ggaaaattgc gcggaggtta taaagaaatc tgtcgtcagt
      421 ttgcgaaatt atttacagat ggcgcagtcg atgttgcacc aatggttggt ttcttgatga
      481 ccttagcgat gttcaataat tcagctgcgt atgcttctcc ttatttttca gctatttttg
      541 gagattggat accgcaatca ccattagtct tggcaatcgt atttgctata ttgacaccac
      601 taggattttt ccgcggtcca atgaatctag tcggttcagg ctcagctatt ttagctgtcg
      661 tgttagctgt taatccaacg atgtcgccag cctttttgtt cccgttattt gcaattacaa
      721 cgattgctcc tcagcattta gatattacac aatcttgggt tgcttgggga ttaggttata
      781 caaaagtaac ttctcgagaa tatatgaaga aatccatccc aactggttgg atcatcggtg
      841 cgatcttatg cctgatcacg ttcttattgt acgggaacgc ctagtaaaca aaacgtcgat
      901 aaaaacgatt agataaatgg aggaaaagaa aatgaaacgt tttgtgcgaa tgggaattga
      961 tgttggcggt acacatacga aagcagtcgc aatcgataat gcgacgcacg aaatcatcgg
     1021 aaaatcttca gtgaaaacaa cccacgatga tgtccgtgga gtagctgcag gtgtcgttca
     1081 gtcttttcaa aattgtttgc gggaaaataa tatcagtcca gaagatgttg tgtttgttgc
     1141 acattctacc acacaagcga ctaatgcact gattgaagga gacgtagcaa aagtcggcgt
     1201 aatcgggatg gcaaaaggtg gattagaagg ttttttagca aaacgacaaa ctagattgaa
     1261 cgatatcgat ttagggaata agaaaaaaat agagattgtc aatgcattct tacctgtcaa
     1321 aaacttaaat gtagaccgtg tttcagaaac gatttcttct ttagaaagag aaagagcgga
     1381 ggtcttggtt tcttctatgg catttggagt agataatggg gaaccagaac gagttgttta
     1441 tgaagcagca tctgtgaaag cgatccctac aacgatggct tctgacatca caaaattata
     1501 tggattgaca agaagaactc ggacagcagc aatcaacgca tctattttac caaaaatgct
     1561 agatacggca acctcgacag aagactccgt acgagaagca ggggtcaatg tttctttgat
     1621 gattatgcgc ggagacggcg gagtaatgga aatcaatgaa atgaaaaaac gacctgtttt
     1681 aaccatgctt tccggtcctg ctgcatcagt catgggctct ttgatgtact tgagagcgtc
     1741 taatggtgtt tattttgaag taggcggaac aacaaccaat atcggggtca ttaaaaatgg
     1801 acgtccagca atcgattatt caattgtagg tggccaccca acatatatct cttcattaga
     1861 tgttcgggtt ctaggtgtgg caggcggttc gatggtacgg gctaatcagt caggaatcat
     1921 cgatgtcggt ccacgttctg cacatattgc cggtctagat tatgcagtat ttacggagac
     1981 agaaaaaatc aaaggtccta aggttgaatt tttctctcct aaagaaggag accctgctga
     2041 ttacgtcaag gtagtgatgg aagacggaga agaagtgact attacaaata catgtgcagc
     2101 caatgttctt ggattagtgc aagaagaaca tttttcttat ggaaatgtcc catctgcacg
     2161 aaaagccata caggcattag cagactattg tcatacgacg gttgaagaca tcgcagagca
     2221 gatcatggaa aaatcctatg ctaagattga accggtgatt ttagaattgg cagacaagta
     2281 tcacttggag aaagatcaga tttctcttgt aggtgtaggg ggcggcgctg cctcattgat
     2341 tacttacttt agtaataaaa tgggtgtgaa atattcgatt cctgaaaacg ccgaagtgat
     2401 ttcttctatt ggtgtagcac ttgcaatggt ccgagatgtt gtagaacgta tcatcccttc
     2461 gccaagtaaa gaagatatac gatcgttgaa aaatgaagcg atgaataaag cgattgaatc
     2521 aggcgctaca ccagaatcga tcgaagtcca cgtagaaatc gatccacata cttcaaaagt
     2581 aacggctatc gctactggtt ctacggaagt gaaagcaacc gatctaacca aggagatcac
     2641 aacagaagaa gcactagagc ttgctgcaga ggatatgcga ttaaataaaa atgaagtttg
     2701 cttattggaa aatacacctt tcttttatgt atgcggcgaa cagaatcgtt caaaaaatgc
     2761 tggaagtctt cggatcatcg atcaaaaagg atttatcaag gtccaacgtg gacatgcttc
     2821 atgtatgaaa acaactgcag ccaattatat gacagctgtc gaacaacttt gggaagatat
     2881 ggcagtttat caaactgaat tgattgcccg accagaattt tatctctgct taggagcaag
     2941 agtctctgat tttacagcga cggatctgga acaactacag cttttgatgg atctggaagt
     3001 atctactatg gaaccggagg aagaagtgat cgtcgttgcc ggaaatatca aacaaacata
     3061 agttagaaga gttactaaac gaattacaac agatccccga agaaatttgg ggattctacc
     3121 agtttcggcg agatttattt tggaaaaaga ttcctttgtc caagcagaaa attcttatac
     3181 aacaatcaat cgattgtggt atcgaaacgg catgtagcat caaaaaaaag tacccttttg
     3241 ccgatgtagg agaaatctgt gaacaaatgg ctgttccaat cgtttcctgt gaatcagagc
     3301 aaataaacga acgaatcact tttgcaactt atgcagaaga tgaagggatc cgattgatga
     3361 cggagccgtt agaaaaatta aaatgttcag gactagcttc gatctctaaa gaaacagcgc
     3421 aagctttgat tattggacat gaactatttc accacatcga agcttctgtt aagggaattt
     3481 atacgcaaag cgaaaaaatt gtgctgtggc ggttgccttt ttatacacat caatcaaaca
     3541 ttcgtgcatt gagcgagata gcagcgatga gtttttcaaa agagatgaat caaagcgatt
     3601 tttcgcctta tgtattagag gcagtcttac tttggccata taatgaaatt cgtagccaag
     3661 ggatcttaga agaaataaaa gaaatagaaa aaagatgtac ggagtatgat ttagattaca
     3721 aataaagaga ctgtgacaaa agtcgtgtca cagtcctttt tttccgaata aacggtgttc
     3781 tcctgtcaac tcctcggtca caagtcaaaa tattcaacaa acatgagaag ctgttttttg
     3841 aaattgttcc ttgtgactcg gagatgaacg gcaggctcac gacctcttgg aaaaaggtgt
     3901 tcaaaagctt gcatctgcgg taataagaac agccaagtta aaaacataag aagctatttt
     3961 tacttggctg ttcttattac ttgatgcagg gcagcttttt cacaacctct caataaacgg
     4021 tgtgcaatca gctgaccctc ggtattagct caaaaaaaac ggcaaaatat gaggaactat
     4081 tttgccgttt atttctctaa tactcaggtc aaaacgctga ttccaaaacc tcttatttga
     4141 tgggtctgtc ctgaatatct tcaaaataat cggggtaatt atgaatcacg tattcttgct
     4201 cctcgataat catatgtaaa tgcatcgtgc aaagaaatcc gacttcatca aagttatgat
     4261 caatcattga ttgaagcatt gtctgatgtt catctaatac tcgaccccag tctaattctg
     4321 aaatcgtcaa acgtaaccat ctgaaacggt tcaagtgcgt actataactt tctagccaat
     4381 cccaaatttc tcgttttcca gcaatttcat aacaagttcg gtgaaaatca gtatccaaat
     4441 caaaaaaagc ttttttattt tgagcgatca ctgcctgatc ggtttgttct agtatttttt
     4501 ctagtttagc ttgatcttgc ttggtcattt tcgcactgca ttcttgcagg atcggttttt
     4561 ccaacttttc ccgcgtataa cgggctaatg aagcagatcg gatatcgatt tttgaaacat
     4621 aagtgccgga ctgtggaatg gtttttacaa gctcttgatt gcgcagttgg atcagtgctt
     4681 ctcgaatcgg tgtacgcccg atagctagtt gttcttctaa cactttatcg gagattttac
     4741 ttccgggtgc taactcggaa taaatgattt gtttacgtat agattcataa gcttgctgtt
     4801 gcaaattatt gataaccatt taaccttcct ttctatgcag atataatcta ttataacata
     4861 aatcgcttac aaatcgtgtg ggcataaaaa cacagtctgc aaaaaaagcc ctgataggca
     4921 tgcctattag agcctcgaaa agaaaaatat tgaatcaatc tggtcgtacc tcttggatcg
     4981 gtttgacacg accgccgatt cttttagcaa aagcttcgga ttcttttttg tctttgaaaa
     5041 tgagcagatt gtctaagttt ttttcccgag tgcgacagcc agttttatcg atgtaaccgt
     5101 gcattacttt aacgacatac atggggatca cctgcttttt tcttttcatt atacgaacta
     5161 ctcgacacaa tacaagaaaa atgcttagac ggtctttatt aagaatttac atcatccaaa
     5221 gaagcgtacg cttggaataa ctgttctaac tccggattga ctttgctcag cttcactaat
     5281 cgttgcgaat cagaaagtaa aaaacagtgt ttactactgc cagtagtttt taattttttg
     5341 tcttcagttc ccacaatttc gtagctaaaa tccaaccgtg ttcccgtata cttttggata
     5401 gttgggtgga tgctcacttt ttctccgtaa tggatcattt ctttatagct gcaatttact
     5461 tccagaacgg ggatgatgat cccagattct tcgatttttt tatagggaaa ccctagatgt
     5521 accaataagg ctacccgagc ctcttcaaac cagcggatat aattgctgtg atgaataatc
     5581 cccatttgat ctgtttcata ataaaaaggt tctcgcacat aacctgtaaa tttctccatg
     5641 actcatgcct cgttttccat tgatttttta caaaaataat tcatccactt cggaagtgct
     5701 tcgctgattt gtgtcggcaa aacaatatat tgttcttttg ccagctggtc tccaatgaaa
     5761 ctatgaagat aaacagctgc tgcaatcgtt tggtcatttt gaggaaactg ggctaaaaat
     5821 cctgtgatca tcccagctaa agtgtcaccc atgccgcctg ttgccatagc agcagaacct
     5881 aaaggattgg tgtagaagtg ctggtgacca taaatttttg tctcatgact tttcaaaacg
     5941 agcacagcac cgatttcctg ttgcttcttt ttgttttctt tatcccgttg ttcttgtatc
     6001 ggtagagaac tcaaccgttg ccactccatt tgatgaggtg taagtacagt ttgttcggga
     6061 tagggtagca aaagatgatt agcaacaagt aaacttaaag cagagccatc aatcaccagc
     6121 cattgttctt ttgattggtt ttctagtact tgtttgaaac gttccaaact cttagaagaa
     6181 gtacccaatc cgggaccaat caagagtaca tctgcttgct ttattacgga aagaaactgg
     6241 tcagtatccg tccaatctac aatcattgct tctggcagat gactgtgaag agcactccga
     6301 tttttttcat cagttactac agtcgtgaga cccgctccac tgtgtacggc agctaaagca
     6361 ctcataatga ttgctccgcc atattgttca ttgccgccaa tcaaaacgat acgtccaaat
     6421 gttcctttat gtgaaatctt tggacgaggt gtgatgactc ttttcactag ttgttcgttt
     6481 atcagcatcg aaaaacctcc ttttactcat tatagcgtat atttttttta gagaaaaaca
     6541 gataagaaaa ggaattgtca gattttttga actatttttt tctgtaattt gtggtatcat
     6601 gaacacgaag acttaaataa gtggaggatt aactttatga caattgattg gcaaaaagaa
     6661 gtagaagcac gtaaagaaga cttgcttgaa gacttgaaaa atcttttacg tgtcaacagc
     6721 gagcgcgatg attcaaaagt aacaccagat gcaccatttg gacctggacc tcgcgacgca
     6781 ttgaaacata tgttagctta tggcgaacgt gatggattta aagtaaaaaa tgtcgataac
     6841 tatgcagggc atattgatct tggcgaagga gacgaaacgc tagggatatt tggacacatg
     6901 gatgtcgttc ctgcaggaga tggctgggac acagatcctt atgaaccagt aatcaaggat
     6961 ggaaagatct ttgcacgcgg atcaagcgat gacaaaggtc caagtatggc agcttactat
     7021 gcgatgaaga tcattaaaga acttgacttg aaactatcaa aaaaagttcg cttcgtagtc
     7081 ggcagcgatg aagaaagcgg ctggggcgat atggcttatt atttcgaaca tgaagaagaa
     7141 ccagattttg gtttctcacc agatgctgaa ttcccaatca tcaatggtga aaaaggaaat
     7201 gtttctttag ctttacgctt caaaggcgac aatgcaggag attatgtctt gaaatctttt
     7261 gtttccggac ttcgcgaaaa tatggtgcca ggaacagcaa ctgcagcact tcaagtacca
     7321 agtgcagatg cagctatcgc gatggaagaa gcattttacc aattcatcga agcaaatcca
     7381 gtaagcggaa cgattgaagc ggacaatacg tatgtaaaaa tcgaattagt tggtaaaggc
     7441 gcacatggtg caagcccaca atctggtatc aatgcaggtt ctttcttggc attgttctta
     7501 gataactatg aattcctagg atctgctaaa caatttatcc atgtagcagc tgcttatgtc
     7561 catgaagatt tttatggaga aaaactagga gtcgcttatg aagatgaaaa aatgggcaaa
     7621 ttgacaatga atgctggact atttgctttt gaagaaaatg gtacagagga agcgaacttc
     7681 atcaatatga atttccgttt tccaaaaggt gtaacagtag acggattgca aagtgacatc
     7741 gaacaaacag tggggcaaga aggcgcaaca gttactcgag gtgctcgcgt aatggaacca
     7801 cattacgtac caatggatga tccattggtg gctacattat tgcaagtcta tgaagaccac
     7861 actggagaaa aaggctacga acaaatcatc ggtggcggaa cttacggacg tttgttgaaa
     7921 cgtggcgttg cttatggagc gatgttccca ggatatactg atacaatgca tcaagcaaac
     7981 gaatttatga gtcttgatga cttgttcaga gcaacagcaa tctatgcaga tgccatctat
     8041 cgtttggcga aataaaaata ctaaaaaa
//
LOCUS       DAZUMK010000117         8066 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000117 DAZUMK010000000
VERSION     DAZUMK010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.119"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(605..1645)
                     /locus_tag="ACG808_002373"
     CDS             complement(605..1645)
                     /locus_tag="ACG808_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFS5047972.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1994..2320)
                     /locus_tag="ACG808_002374"
     CDS             complement(1994..2320)
                     /locus_tag="ACG808_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047973.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2307..3095)
                     /locus_tag="ACG808_002375"
     CDS             complement(2307..3095)
                     /locus_tag="ACG808_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5047974.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3463..3753)
                     /locus_tag="ACG808_002376"
     CDS             complement(3463..3753)
                     /locus_tag="ACG808_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFS5047975.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(3889..4068)
                     /locus_tag="ACG808_002377"
     CDS             complement(3889..4068)
                     /locus_tag="ACG808_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047976.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4065..4415)
                     /locus_tag="ACG808_002378"
     CDS             complement(4065..4415)
                     /locus_tag="ACG808_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047977.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4408..5733)
                     /locus_tag="ACG808_002379"
     CDS             complement(4408..5733)
                     /locus_tag="ACG808_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFS5047978.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            6183..6797
                     /locus_tag="ACG808_002380"
     CDS             6183..6797
                     /locus_tag="ACG808_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5047979.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(6962..7786)
                     /locus_tag="ACG808_002381"
     CDS             complement(6962..7786)
                     /locus_tag="ACG808_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5047980.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
BASE COUNT         2491 a         1432 c         1167 g         2976 t
ORIGIN      
        1 cgtaagggat tttattcttt atttaaaact ttgcaacaga accgtccgtt gcgggtaata
       61 ccgtaacact gaagggcacg cattaaggcg tcatcttaat gctttcctgt aggacactga
      121 cggagattac cagtctcccg agccttacag gggcctttct tttttttgta ttttcaatta
      181 tcacaattta tcgtccaaaa gtgcagaacg aaaatcaatt catcacaagc atatcagaca
      241 attaaattta aaacaactaa aaatatacaa aaaagtgtaa ttgaatatta gcagcactgc
      301 ctgaaaagaa gtaatatcag gcttttcagc ttggggaaat aagtgaatta cctctcttca
      361 tgaattatta taactcaaaa aagcacatat gcaaacacta gttccctatt tttttgaatg
      421 aaaaataact aaaatatcta acgattatga ctttaaaacc tacgtttgtg attgttttca
      481 taaactttta atctaaaaac tccggaataa tagctattta tttgtaacat ttgtaataaa
      541 attaaaatca taaaaaagtt ctaatttaaa attgatcatt tttatagtga tcgcttttta
      601 tcaactactc tagccaattt tccgtactaa ctgttggtaa ttcgttaaat gtttctcttt
      661 gttcgtttgc aagctttgcc gcaatagctt tttcgacaaa ccaatttttt acataaacga
      721 aaattagatt ctgcaaattg tttacttttt cagttttttg tttttgataa gctttcagca
      781 aagtcatata taatcctttg tcagcatcaa ttccataaga ttccagttct tcataaacaa
      841 caactgtttg ctctaattct tcagcttttt tctttgcatt atgaatagtt tggacggttt
      901 tttgagcgtc ttcgtaagta ctagaaaatg tttttatcaa gtttaataca ttttctggta
      961 caaatgtatt tatactgtcc tctttcatga tgtcgacgaa gttatctagt aaaacttgat
     1021 tttgtaactg atcagtttca gtgtctatta tgtgtctatt agtgtctata gaattagaat
     1081 agaatagatt gtctgcgatt ttcgcaatta cggttgagtc cttgatatga ctggattttg
     1141 ccgtatttgc gattttcgca attacggtgg tatcattgat ttctcgcggt tttgccgtat
     1201 ttgcgatttt cgcaattacg gtggtatcag tattgtcttc ctcttccatg attttaaaga
     1261 cgtcttgatt cgttacttct ggcttgccta agtacaataa attaggcatg gattcaatct
     1321 taccgtcacg tttatttact cttcctctct tctgtttcag aagacctgct ttttctaatt
     1381 cctttttgat tttagatact ttaccttcgc ggcattgtaa caatttcatt aattcttcaa
     1441 ctgtgaatat gaaaaatata ttatcatcag aatctaccca gttatttttg actgaataat
     1501 caaatcgatc tttaagtagc atataagcaa tttttgcatc atttgataga tccatgtatt
     1561 tttcgttagt aaaaaatacc ttaggcattt gataatattt ttcacgataa atttcttttg
     1621 atttgtaata attaaaatca ttcatagcca atcctctttc tgtaggatag cgttttgttc
     1681 aaaactatgt tacaatgaat taaacaaata tatctatcca ctatatattt ttctactgct
     1741 gataagtcgc caaactttgt taaagcagta gtcttttttt atcttttttt agtttacctt
     1801 aaaaaataaa ttaaagcaaa ccgctctatt tcaaggttta agctcatagt aactatatca
     1861 cgatattaca ataatattat atcacgatat cgtaatatta caatatgaag ataagcgact
     1921 tatcagcttt gatactactg atttttataa aaaaaatatc acgatattat gatatcgtga
     1981 tattgaatgt aaattaaatt ttattttgtt tctttgctag tttatatcct tcaatcaagt
     2041 attcttcagg tatggacttt aatagcgcat cttctataac gtctggttta cttttgtctg
     2101 attcagcaat agtcgtccaa tatttgagtg ctgacattag aattggattt atacgaatgc
     2161 tatctgctgt tcttacacta cttccttgat cttttatgac tttacttata tttttcttgg
     2221 ttaattcgat ttcttctact tcttttgatt taaaattctt tttattttta tttcctagta
     2281 atctacttac tgcagcttga ctgcgattat cttctgccat caaaatcacc aacttctttt
     2341 aaatattctt ctgtaaatgc tttatagtca tcttcagctg ttgagggttt attaaaattg
     2401 attagatctg atccagcagc ttgcgcttcg tcaacggtta ttgtcctacg aatataagtt
     2461 ttgaaaattt ttgcaccaat gactctagaa atttcggttg ctgcagacgt catttcttta
     2521 gaaatgttat aacgcttatt atacttagta aatagaactc ctcgtacttc aagcttgtcg
     2581 ttcgtatatt cttttaccgt ttcaatagtt tcacttaact ttattagtcc ttttaatgag
     2641 aaatacgcag cttcaactgg aatgataatt tcatcagaaa cagtaaatgc gttgactgta
     2701 agcattccta aacttggtgg acaatcaatt ataatataat catagttgtc cttgattggt
     2761 tgaagtactc ttttaagcct gtgttcactt cctacttgag taaattccct ctctgcagaa
     2821 gaaagggcta tgtcggctgg caaaacatca attcctttat agtccagaat agcatctgta
     2881 atgtcagcct ccccttttaa tacttcataa attgtaggtt ggtttgttcc atctatatcg
     2941 aaacctacat tatctgtagc atttccctga ggatcaatgt ccactaaaag tacattttta
     3001 ccgtaaacaa atttcaaaca tgctgctaaa ttaacgctag tcgtagtttt agaaactcca
     3061 cctttttgat tggctactga caatattctt gccattaaaa tatcccccat tcatcttata
     3121 ttataatata attatatcat aatattgtga tattgcaata tcgtatatgc attatattgt
     3181 aatattgtgt gattattttg ttttaaagat atatatcggt tcggatataa agattattca
     3241 tatgttattc gaaattaaaa taatcacttt ttattacttt aaaatttttt attaatgata
     3301 agaaatatgt tttgcgagca gcaactagaa taatttaaac attactaaac tgtctatatt
     3361 gttactaata attcccttct tatagtgcat tttcttagaa aaaaacaata ttacgatatc
     3421 atgatattaa gatatcgtaa tattatacaa ttgtgattga tattatatat gtttttttat
     3481 ttttttatca tgtttaatct tgaaggaatc atattctttt tctaaagaca ggagcttttc
     3541 ataaacatca gacatgttta aactagaggc caggcttaaa tcataaacga atgaagcagg
     3601 taatgatttt attctacgtt gtcgggttat ccagcttgca accgttcctt gtgggtaggc
     3661 atataactca caaaattgtt ctacagtgat tcctaaattc ttgataatgt aaacattgat
     3721 cgggtgcgag taaacaaatg tattgataga catggtgcgt aagcctactt tcttttatta
     3781 ttgcgtttgc aataatagtt taacacacgc attattctag tatctactcc tatttgtaaa
     3841 aacaaaaaag gattcataga gataactcta tagaatccgt aaaatacctt atatatataa
     3901 cgggaagata taaaacctaa aattatgata acctgctgca gcttctacat agaaagagaa
     3961 gcgctcaaca ccaacctcat ttatcaaacg ttggccataa ccccaaatct cttctgatag
     4021 ctgatcaaag gttatatact ttagctcata ttgctggata atgttcattc taatcgactc
     4081 catttattat cctggtaaaa ttcgatatga cgtatttcgt cgtagtgaat tttgtctttt
     4141 ccaacgtaga tccccagctc atcactacct aagatcttgc caatgatatc ttctggatag
     4201 tatccttcag cgtttcgttc ttctttttga atagatactg atctattttt tatgactgca
     4261 gcgttcaata cttccgcaat ctcttcagtc gtcatttgtt cttttgctaa attttgactg
     4321 gttcgttgtt cattgtcggc atcaatttct tcagtatgat cagataaata aaaccctgcc
     4381 cactttaaac gtccacgatc attgtaatca atcattttta attcccccat ttccgcccgc
     4441 atgaccacca acaagtgtgc ttctagtaat gcttctagcg ccttctaaca ttgaagaagc
     4501 atgtacaatc gacgtaaaac catatttttg ccgaatcttg tcaataattt tatctaaatt
     4561 ttcttcgtca atttgctttt gtggatcact aaataaatcc agctgaagcg aatcggtgta
     4621 cacaagcttg ctataggtta ttccaacatg ccttacttct tgtccttcat aatacttatc
     4681 aaacaagaaa agacaataat tctgcaattc ctttgtatta tctgtgatag ggatcttcat
     4741 ctggtgtgaa aaccctggtc tcgtttctaa gatcgacgtg ccaatgtgta aatggacaca
     4801 acctgtttta cagttatgct gcctaatgcg tatcgctact tgttccgcca tttcttttac
     4861 aaccagctcg atttcatttc ttctagtata atctcgtggt aacacttgtg aatttccata
     4921 tgatttttcc tttgtcggtt ctggtgggat tgctatatct gttcgatcga tcccattagc
     4981 atggaagtac agctgtaagc caatcacacc tagtctattc ttaatcgtat aaggattcca
     5041 gttagcaaga tctctaatgc tcataatccc catctgattg agtctttttt tcattcgaga
     5101 gccaattccc caaaagtccg tcatttcagg aatattccat actttttcag gaacgttttc
     5161 gtacgtccat tccgcaacaa atccttcatt atgctttgct tcgttgtcta aggctagttt
     5221 agccaaaagg ggattatctc cgacacctac tgtagcaatc aatcctagct cttctttaat
     5281 attctcctgg atcatttgag ccagcttctt tctccgttga cttcgtgttc cttcagtcgt
     5341 aaaaagattc agtgatcggg tcacttttaa gattgattca tcgatcgagt aaatcagtag
     5401 atcttcatca gccacatatc ttctgaaaat attatttacc tgcatatttc gcttgatata
     5461 taggttcata cgtggtggaa caacgtgtag tgttttagga aatggttgtg gtaagtcacg
     5521 tggtctactc acatttgtaa taccataccg cttttttgct tcaggagaag aagctaatat
     5581 caatcctgaa ccagtattat cagctcgact cataacaaca agttctgttg ttaatggatc
     5641 taaatttctt tctatacatt cgacacttgc gtaaaagctt ttcatatcga ttaaaaagta
     5701 atcatttact ggctcttttg aataatccat cataaataac actccattct ttcacattat
     5761 acaaacatat gttcgatatt gtaaagaaga aatagctaaa tacataaaaa aagaagcata
     5821 ggtatgctat caccctgctt cttcttttga tttgtatgat caatttattc tatcacttta
     5881 ctaattaatt tgaaaatcta ataaaaactc tcactctaaa agagtgagag actgcaaatt
     5941 agagtacgga cttttttgga gttccgttct ttaatattaa caaatgaata aaatccttac
     6001 aacatttgag aataagttat cagtagacac ttacaaaaaa ttctcatact atttcctcca
     6061 tcattagttt ttgtctatat aaggtttacc ctaaatagac actggtataa cattgaaaaa
     6121 tatggcttcc tcgtgtatga ttagggtgta ttttaaatag ttgcagaaag taggtaaaag
     6181 ttttgagagt tgcatacata cgtgtctcat cgatcgatca aaatgaacaa cgccagattg
     6241 aagaaatgaa aaaattcggt gcagagagaa ttttcatcga gaaacaatct ggcgcaacga
     6301 ttactcatcg acctgttttc caagaagcat tagattacgt ccgagatcaa gatattttta
     6361 ttgtggaagc tattgatcga cttggaagaa attatgacga gatcatcgct tcagtcaatt
     6421 acttgaagaa gaaaaatgtg aagctgatca ttactagttt accgatcatg gctgaagcaa
     6481 ttggcaatcc tctcctggat aaattcatta aggatctgat catccaaatt ttggccatga
     6541 tcgctgaaca agaacggact gaatcgaaac gtcgccaggc acaaggaatt aagatcgcaa
     6601 aagcaaacgg cgtctacaaa gggcggccca aattatacag tgcagacgca aaagatcccc
     6661 aacgtcgcct tgtttacaaa agcatcgttg aagatcttaa aaatggtgtt gcgattgcta
     6721 agattgctaa agactacaat gttacgagac aaaccgtgta tagaataaaa aaagacagca
     6781 tggtcaatga caaatagttg atcatgctgc ttttattatc ttctcatctt taatttccta
     6841 catataatta ctatctataa aattaagtct tactcagaac agataaagca taaaattcca
     6901 acaaaccatt ttttacttaa ctaaataaag agtctgttaa tataatcttc acttatttac
     6961 gttaatcccc tactgtattt gttggttcct tatctatatt ttctacaaaa tccttattat
     7021 cttgtgattt agcttcgtat aattctttgt atgtcgacac attggttata ctaattccta
     7081 tagctagttc ttgagactct acttttctct ctagctcctt tgacaaagtg atttgagatt
     7141 tatttggttc cgttggagtc aattctatag attcttcata aaaatctcta tacataaagc
     7201 tatcattaat tttataaaaa aaattaaatg tttttagcac tgccatataa cattcaaaac
     7261 attcttctat agttggttta cttccttgag gagaatgaac caatttatta cgtttttcta
     7321 tgaaattttt aatttccttt cttatttctt tcggaaactc aatattaaag taccctttaa
     7381 acatgctccc taataatttg tctacagggg gagattgtaa attctcaaat attagagtta
     7441 aatcaggttt ctcttgttga taaaattcct ttacccctat ttcacaggct gtaattatcg
     7501 aaataaacct atctctagta tttgatatat ttttacttct tttaaggagt ataagagaaa
     7561 aggggatact ttcgtgtcca tgcatgtaat taactaattg ttgagcttca gaagttatat
     7621 ctctaattat atagttaacg gaattaggaa gtgtctttaa ctctaatgaa atttctatcc
     7681 aattactatc ttctaatctg tatatgctcg aagtataatt tgattcgtca gcaagcatcg
     7741 ctacatccca aacactgatc ataaaatctt ttaaacgttc aatcatttca gcaatattgc
     7801 taaaatcagc taatttttct ttagaataaa aaaaaacatt tatatcagaa tcaataaatt
     7861 ctattttttt attactatat cgtcatcatt atcttgacca tttatgttta ttgttccctg
     7921 agtggggagt acttctttca aattcaagtt aaatttatat tccattactt ctcctccaat
     7981 atataaaaat tacttaactg tatccaaatt taatatgtaa ataaatcatt aatttttagc
     8041 taaattctat gagaagttta tttttg
//
LOCUS       DAZUMK010000118         7989 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000118 DAZUMK010000000
VERSION     DAZUMK010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.120"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            225..1763
                     /locus_tag="ACG808_002382"
     CDS             225..1763
                     /locus_tag="ACG808_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFS5047981.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            1760..2725
                     /locus_tag="ACG808_002383"
     CDS             1760..2725
                     /locus_tag="ACG808_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5047982.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            2712..4034
                     /locus_tag="ACG808_002384"
     CDS             2712..4034
                     /locus_tag="ACG808_002384"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS5047983.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      4097..4201
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4275..5018
                     /locus_tag="ACG808_002385"
     CDS             4275..5018
                     /locus_tag="ACG808_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5047984.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            5033..6025
                     /locus_tag="ACG808_002386"
     CDS             5033..6025
                     /locus_tag="ACG808_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5047985.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            6037..6774
                     /locus_tag="ACG808_002387"
     CDS             6037..6774
                     /locus_tag="ACG808_002387"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5047986.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            6764..7486
                     /locus_tag="ACG808_002388"
     CDS             6764..7486
                     /locus_tag="ACG808_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFS5047987.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(7570..>7989)
                     /locus_tag="ACG808_002389"
     CDS             complement(7570..>7989)
                     /locus_tag="ACG808_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFS5047988.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         2553 a         1388 c         1744 g         2304 t
ORIGIN      
        1 tgattccaag tgacaatttc gtatagattt ttatggtatt cgtagtgaaa aaacgagaac
       61 caaaaagttg atccgacttt gttgcttgga ttcttttttt gtcagcatat ttagaggtat
      121 ttttttcttt tttttgttcg tgctgtaagg ttaaggcaag acaagttagt tgggaaaaag
      181 gaaagaaatg tttatactta aagaataaca gaaaggaagg tcgcatgaat aattttatac
      241 aaacattaag tgaaagaaaa ggcgagttgc tggaagctgc atttcaacat ctgtccattt
      301 ctctcatttc tttgctgatt gcagcactga ttgctattcc tttagcgatt tgggcagcca
      361 atcataaaaa aatggcagaa gtcttattgc agatcacggg tgtgctccaa acgattcctt
      421 cacttgcctt attaggttta cttatcccat ttgtagggat cggaactgtt ccagcattga
      481 ttgcacttgt tatttacgct ttgcttccga ttttccaaaa cacgtatatt ggtctagctg
      541 aaattgaccc gtctattgaa gaagcagcgg tagcatttgg tatgtccaga atgagacgct
      601 tgctgaaagt agagcttccc atcgcattac ctgtcattat ctcaggtatc cgaactgcct
      661 tagttttaat catcggtacg gctactttgg cagcattgat cggagcaggt ggattaggga
      721 catttatttt gttaggtatt gaccgaaata cacctgtttt gacattgatt ggtgcgatca
      781 gttctgcctt gcttgcaatt atattcagcg gactgattcg ctggttgcag cataagaaac
      841 ctcgatattc catcattact ttagcagcag tggtgtgttt atttggcggc gtttctttat
      901 atcagtctgc cttgtttaaa aatgaaacca tcacgattgc tggaaaatta ggagcggaac
      961 ctgaaatcct gatccaaatg tataaagaat tgattgaaga caacacaaaa acagaagttg
     1021 aattaaaacc gaattttggc aagacgagtt ttttgttcaa tgcactggat caaggtcaaa
     1081 tcgatatcta tcctgagttc acaggtactg tcctagaaag cttggtgaag gtacctgaag
     1141 caatgaaaag tgctgatttg acaaaagaaa aaacatatga cgaagcagaa gaattattga
     1201 ataagcaatt cgatatgaca ttattaaaac caatggctta ccaaaatacg tatgccttgg
     1261 cagtcaagag atcttttgcg aacgaacatc aacttaaaaa gatttccgat ttggcagcta
     1321 tctctgatca gatcaaagcg ggattcacac tggaatttat tgatcgttca gatggatata
     1381 aaggaatcca agaagcatat ggcttgaatt tttcaagtgt acaaagcatg gagccaagtt
     1441 tgcgttacca agcaatcaat aatggagatg tcaatattgt ggatgcctat tccacagata
     1501 gccagctcaa ggagtacgat ctagtgactc tagaagatga tcgagggtta ttcccagctt
     1561 atcaaggcgc accgctgatg aaaaaagatt ttgctaaaga acatcctgaa atcgttaaag
     1621 cactgaataa attaagcgga aaaataacag aggatcaaat gattgatatg aattaccaag
     1681 tcaatgtcga aaagaaaaag ccgacagaag tagcacgaat cttcctgaaa aatgaaggat
     1741 tagtaaagga ggaagatcag tgactaaatt aatcgagttc aaacatgtgc aaaaaaagta
     1801 tgatggaaag tatgtcatcg atgacctaaa tctggcaatc aaaaaaggag aaatctttgt
     1861 tttggttggt ccttccggta gtggaaaaac aactactttg aaaatgatca atggattatc
     1921 taaaccatca gcaggagaca tctacttcaa aggaaaatcg ttaaatgaat ataatctgca
     1981 gaaaatgcgg tggaatatgg gatatgtgct gcagcaaatc gctttgttcc cgaccatgac
     2041 agtcaagcaa aatattgaag tcatccctga aatgttaggt tgggaaaagc agaaaagagc
     2101 agaccgtgta gatgaacttc ttcaaaaagt tggcttatct cctgatattt atcgagatcg
     2161 tatgcctaga gagttgtctg gaggagaaca gcaacggatc gggatcatcc gagctattgc
     2221 tgcaagccca gatgtgattt tgatggacga gccatttagc gcattagatc cgatttcaag
     2281 aaactcatta caagaattgg ttttgtcctt acacgaagaa ttgggcacga ccatcgtttt
     2341 cgttacgcac aatatggaag aagcaatcaa gctaggtgat cgtattgcat ttatgaagga
     2401 tggagaaatt atccaatgtg acacaccaga gcagttattg atgaatccta aaaacgacta
     2461 cgttcggcat ttctttgatg aaccgaaaca gacgaaagag tggcgagtag aagatttagt
     2521 cgtcaatgga tatttcttaa atgaaatacc agaaaatgct cgtcaacaag tttgctttga
     2581 tacgcctatg aaagaagtat attcgcttct ttcggtgtat ccaagcattg tgatagtaga
     2641 aaagcaaaga agtattgggt cgctgacaag taaagaaatc tttgcttttt taagtcgaga
     2701 ggaggaagga aatgaaaaca tatgatgcaa tcatcatcgg ttcaggtgtg agcggcttaa
     2761 gtgctgctta tggattgaaa gaagcaggta aaacagtact agtggtcgaa gaagatctat
     2821 ggggcgggac ttgtcctaac cgtggatgtg atcctaaaaa agtcttgtta agtgcagtag
     2881 aggcacgtaa tcgggtaaaa caattgagtg gaaaaggttt taacgagatc ccaactgcca
     2941 actgggaaga acttcaaaaa ttcaaacgaa catttacgga tccagtccca gaaagccgaa
     3001 aaaaacaatt agcagaagct gaaatcgatc atctttcagg aaccgctcgt tttttggatg
     3061 attcatctat tgaagtcaac gaagaagtct tccatgcgga ttacttagtt ttggcaaccg
     3121 ggcaaagacc tacgatctta ccagtggagg gaaaggaata cttgaaaact agtgcagact
     3181 ttttatcatt gcctgtactg cctaaagaaa tcatttttat cggtggtggt tatattgctt
     3241 ttgagctagc tacgattgcc aatgctgcag ggagcaaagt aaccattgtc catcataatc
     3301 aacggccatt aaaagaattt gaggcgtctt tggtagagga agccgtccat caaatggaag
     3361 catctggcat tcagtttgct tttggtgttg aaacccaaaa gatcatttca gaaggtactc
     3421 gctatcgttt agtcggaaaa gagacggaat tagtagcaga tatgattttt tgtgcgactg
     3481 ggagacagcc gaatacagaa tctttagcac ttgagcaagc gaatatcgtc tttgataaac
     3541 acggaattgc agtcaatgat tatctgcaaa cgagcaatcc aaaaattttt gcgtgtggag
     3601 atattgtttc aaggaaaacc ccaaaattga caccagtcgc cacttttgaa ggaaattatg
     3661 tagctaagcg gataacggat gcaacaagcg aacctatcaa atatccgatt atacctacga
     3721 tcgtctacgc cagtcctaaa cttgctgaag taggagttac aaaaagtcac gcctcttcgt
     3781 cagatcaagt ggtagagatg gatcttacca gttggttcac ctatcatcgt gtcaatgaac
     3841 cagttgctaa ggctgaattg acctttgacc agcaaaatta tttgattggt gctgcagtga
     3901 ttagcgagca agcagacgaa ttgatcgatg atttgacact cgtcatcaat caaaaattga
     3961 ctaaaaaaga gttggatagc tatatcatgg ggtatccgac attggcaagt gatctctcct
     4021 atttattgaa ataatcagca tgataactaa agcgcttgtt gctttttcct tcttgtgcta
     4081 cactttaaaa aatgaatacc tgtttctggg gatgtcgtag acgacatgag agtaagttgt
     4141 gaacttagac ccattgaacc tgtcagttaa tactggtgaa gggagaaaca tagcagacgc
     4201 ttgaagcggg ttactatgtc gtgcccattt caagcgtttt ttgttattca tattagaaga
     4261 aaggaggtaa caaaatgaag aaaaatcttt atccagcagt cagtatggca ttcattttaa
     4321 ttatttggca gattgttgtc atagtttgga aaatacctgc ctttatttta cccggacctg
     4381 ctgctgtatt ttctgcatta gcggcagatg cacaagtatt actttcccat tctgtcacga
     4441 cattaaatga agcagtgctt ggtttggtaa tagctagcgt tctttctttt attactgcct
     4501 tggcaatgga ttattggaag tttttagaaa tgtcttttta tccgttacta gtgatttcac
     4561 aaatgttgcc aatcatggtg ttagggccat tacttaccct atggtttggt ttcggtatgc
     4621 ttccaaaagt gatccttgtt gtcttgataa gctattttcc gatcgtagtt tctttttcgg
     4681 atacattgag gaaagtatca aaagagcaaa tcatcttttt gaaaacgatg ggggcagata
     4741 cagggaagat ttatcgaata tataaaatac cagcgggttt atccggcttc ttttccggtt
     4801 taaaagtggc tgctacttat tgtgtcagcg gagcagtcgt tggagaatgg ctaagcgctc
     4861 aagcaggctt agggtattac atgatacgcg taaaaaacag ctaccagatc gataaagttt
     4921 ttgctgcgat catttgtgtc attctattga gtttattgct gaatggcgct tgttcattat
     4981 tgaaaaaagg gtattatcgt atgctttata ttttttaaga gaaggggatc aaatgaaaaa
     5041 attatggctt ttattaccgc tactgttgct tttatccgct tgtggcactg caaaagaaac
     5101 aagtagcaaa caagaaataa aggatttgaa aaaagtgaca cttgttttgg attatgtacc
     5161 gaatactaat catactggca tctacctagc aaaagaaaaa ggctattata aagaggctgg
     5221 tttaaatgtc caaatcatcg aaccaggaga taattcgaca agtattggtc tagtaggtgc
     5281 ggacaaagca caatttggtg taagttatca agaagatgtc acttatgctc acgcagatgg
     5341 acagaatata ccagtcaaag caattgctac agtgatcaaa cataatactt ctggattcgc
     5401 caccctatca gacagtaaca tccattcacc aaaagatttt gaaggaaaaa catatgcagg
     5461 ttggcaaagt ccaagtgaag aagctgtctt aaaagctgtg atggaaaaag acggcgggga
     5521 cttttcaaaa ctgacaatgg taggaagcaa tggagaaggg ccagaaagct taggaaaatc
     5581 ctcagatatc caatggtact ttgaaggttg ggatatgatc aaagcaaaag aagcgggtat
     5641 tgaactaaac tatatcccat tgaaagaatt agatgaacgg ttagattact atactccggt
     5701 aattattacg aatgatcaac tgatcaaaag tgatccagaa ttggtacagt cttttatgga
     5761 tgccacgaaa aaaggctatc aggaagctat taaggatcca aatgacagtg ctaaactgtt
     5821 gcaaaaatat gcaaaagaaa atgatcggac gtttttagaa gaatcacaag cattcttgtc
     5881 aaaaaattat acggatgatc ctgagaattg gggattgatg gaagaaaaag tttggcgcaa
     5941 ttatacagct tttatgcagg aaaacggatt gatcaagcaa gatgtacctt cgcaagaact
     6001 attcactaat cagtttataa aataggagtg atagatatgt caatccaaat cagccacttg
     6061 aatgtagtca ttgaaaacct gaaaatatta gaagacatcc atttcactat ccctaaagat
     6121 acctttacat cgattgtcgc gccaagcgga gccggtaagt caacattgct gaaaacttta
     6181 acaggagtcc agcctatgac ttctggaacg atcaaagtag acgatcaaaa agtaactggt
     6241 ctccatacag catttagtta tatgccgcaa gaagatatgt tacttccttg gctgagcgtc
     6301 tatcaaaacg ttacgttata tcaaaaaatc aatcatctaa caatcaatga agaacaagta
     6361 agggattatc tggatatctt tgggttaaac ggctatgaac attttttgcc ggaacaatta
     6421 tctggcggga tgaagcagcg aacggccttg ttacgaacaa tcatgaatcc ttcttcttac
     6481 ttgcttcttg atgaaccatt tggagcgtta gatgctatga ccagagggca gatgcaagac
     6541 tggatgttga agctgcctaa aaaagcaaag cggactactt tacttgtgac gcatgatatc
     6601 gaagaagcaa tttatttatc agacaggatt cttgtgcttt ccgcccgtcc ggcacatgtg
     6661 attgctgaga ttcaagtgcc tgaaaaacga cggtcacgtg aatggctact gcagcaatca
     6721 gagttgaagc aaacgattta tcagttattg gcaggtgaat tagatgttaa gtgatgctca
     6781 ttgtcattta gatgggagtc agagtttagc actccttcag caggaacaca cgattttgac
     6841 gatcatcaat tgtgattcgc cagaagaatg gaaggaaaac agacagcttg cagcaagtaa
     6901 gactcaagcc ttaagttacg gtatccatcc ttggaaagca gatagctata cgtttgaaca
     6961 agttgagcct tttttgaaaa aagcaaggat catcggtgaa atagggttag ataatatttg
     7021 gacgaatgta ccgatgacta cacaaaaaaa agtatttgag cgacagcttg cttttgcagc
     7081 cataaatgag aaacctgttg tactgcacac aaaaggatgc gaaaaagaga tcttaaatta
     7141 tatccaagct taccctaatc actatttgat ccattggtat tcttctttgg actatcaaca
     7201 agactatatt gatttaggct gttatttcag cattggtctt gatttgaaaa agaatccagc
     7261 agtctggcaa ctagctaaag cagtcccaat cgaccactta cttattgaaa cggatggact
     7321 acatgcagca gagtgggctt taggggggat tttttcagaa aaagattatg tctcactgct
     7381 tgaagaacag ttaaagacaa tcgctgagat caaagagcta tcagttgaag aagttaaaaa
     7441 aaatacttat caaaacttgg aacgtttcat tagattaggt aaataaaaaa gagatgtgac
     7501 acaactagtg tcgcacctct ttttggcttt ctttgtataa aagtaaactg tttgattaat
     7561 aaatgaatct tagtaccaac cgtttgcttc ccagaatgct ttcgccgctt cccaagaacc
     7621 ataacgtgaa gtaacatatt gttctgctac tctttcttgg tttgcagcag agtagtcacc
     7681 atttaagtaa gaagaatcta attggtaacg tccgatgtaa cgtccgttcg ttgctgtgta
     7741 agaaccgctt gattcttttt gtgcgatcca ttcttttgca gagcttgtgc ttgctggagt
     7801 agcagcagtt tctgttacag caggagcagg ttgtgtttca gcaacaggcg ctgcttgttt
     7861 ttccgtttga accgtttctt gtacaacagg ttgttcagct tggactggtg cttcttgaac
     7921 ggctgcttga ggttcagcag ctgcttgtgc agagccttct gttgggattg ttaattgttg
     7981 tcctgagta
//
LOCUS       DAZUMK010000119         7933 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000119 DAZUMK010000000
VERSION     DAZUMK010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.121"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            226..699
                     /locus_tag="ACG808_002390"
     CDS             226..699
                     /locus_tag="ACG808_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFS5047989.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            696..2828
                     /gene="feoB"
                     /locus_tag="ACG808_002391"
     CDS             696..2828
                     /gene="feoB"
                     /locus_tag="ACG808_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFS5047990.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            2830..2964
                     /locus_tag="ACG808_002392"
     CDS             2830..2964
                     /locus_tag="ACG808_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFS5047991.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            3058..3702
                     /locus_tag="ACG808_002393"
     CDS             3058..3702
                     /locus_tag="ACG808_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5047992.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            3889..5082
                     /locus_tag="ACG808_002394"
     CDS             3889..5082
                     /locus_tag="ACG808_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS5047993.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            5075..6802
                     /locus_tag="ACG808_002395"
     CDS             5075..6802
                     /locus_tag="ACG808_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFS5047994.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            7196..7393
                     /locus_tag="ACG808_002396"
     CDS             7196..7393
                     /locus_tag="ACG808_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFS5047995.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            7395..7706
                     /locus_tag="ACG808_002397"
     CDS             7395..7706
                     /locus_tag="ACG808_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFS5047996.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            7810..>7933
                     /locus_tag="ACG808_002398"
     CDS             7810..>7933
                     /locus_tag="ACG808_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HFS5047997.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVF"
BASE COUNT         2633 a         1319 c         1727 g         2254 t
ORIGIN      
        1 aatagagaaa aaattgtgga aaactatgaa gaaatatttt tttctcattt ttatctatca
       61 atactaaaga ttcgctcata atatcacaag tataattgac attctcaatt accgagagta
      121 ttctatgtga gaatgattct catttatttg cgtaacacgt aagaaaatta gaacatataa
      181 tgaatgattt taatacatga tttatttcac aagagaggaa tcaatatgca gaccttagcg
      241 caggcaaagc agaatttcgt ttatcaagtc gaacgaatcg aaacagaaga aaaaacaaaa
      301 cgccatttgc aaaatatcgg gatcgttgcc ggaagtaaaa tcgtcgtcgt caatttatca
      361 ggcaacaatg ccattttact ggtaaaaagt catcgagtgg cagtaagtcg ggaaatacta
      421 ggtcaaatct ttgtcaataa aaagactgct tcctctgaaa gctggacttc tcttgatcaa
      481 ttaaaagcag gagagactgg gatcgtcgta gcaatccacg gacaaggcgc agtgaagcga
      541 cggttaatgg atatgggatt gacgcgaaat gtaaaaatag ttatccgaaa actagcccct
      601 ctgggagatc caatcgaact gacagtgcga gggtatgaac tgacgttacg aaagtcagag
      661 gccgaactaa tcttgataca aagggaggaa aaagaatgaa tatcgcgtta gtcggaaatc
      721 caaacagcgg gaaaactagc ttgttcaatc tcttgactgg ttcgaatcaa tatgtaggga
      781 attggccggg cgtaacagta gaaaaaaaag aaggatcgtt gaaaaaaaat ccgaacattc
      841 atattcagga cctacccggc atctattctt tgtctcctta tacaccagaa gaagtgattg
      901 caagagatta tttggttcag gaacagcctg aactgatcat caacattatt gatgctacca
      961 atttagaacg aaatctttat ttaactacgc agttattgga tttagacatt cctatgatca
     1021 ttggtctgaa tatgatggat gtcatcaaaa aagaaggaaa aaaaatcaat ctagaaaagc
     1081 tttcctatgg tcttggtgta ccagttttac caatcagcgt attgaaaaag caaggaatcg
     1141 aaaaactaat cgaaaagatt caggagtctg caaaaaagcg gccttctatt cctgttccct
     1201 gtacatatga tccacgttta gaagcagctt tacatgaaat tgaagatgta ctaggagaac
     1261 aaagcggcaa gaaacgctgg tatgcaatga aatattttga acgagatcag aaggtaagag
     1321 aggattacga gatagcagtt tctcagcaaa aagagatcga gcaactgatc cagttaacag
     1381 agaaactatt ggacgacgat agtgagacga ttctggtcaa tgaacgttat gaatttatta
     1441 cccaactatg tacactctgt gttgtgtcaa atgattcttt ccaattatcg atgagtgaca
     1501 aaatcgatca gattgcaacg aatcgttggt tagccttgcc gatcttcgca tttgtgatgt
     1561 ggctgatcta ttatctggct attcaaacag taggaacaat ggggacagat tggatcaatg
     1621 acacattgtt tggtacatgg ttaccagaac atgttggcag attgttagct gaatggcaag
     1681 tagcgggatg gatgcaggag ctgatcttga acggcatcat cgcaggcgtg ggagcagtgt
     1741 taggatttct accgcaattg attatattgt ttttatgctt gagctttctg gaagactgcg
     1801 gatatatggc gaggatcgct tttgtcatgg atcgactttt caggaaattc ggtctttcag
     1861 gaaaatcctt cattcctatg ctgattgcta caggatgtgg agtgccaggc gtcatggcca
     1921 gtcgcacgat tgaaaacgag caagatcgtc gtttgacgat catggtcaca accttcatgc
     1981 cttgttctgc aaagttgccg attattgcct tagtagcagg ggcctttttt cctcatcaat
     2041 cttgggtagc tccttctgcc tattttcttg gtatgactgc aattattttc tctggaatca
     2101 gcttgaaaaa aacacgtttg tttgctggag atacagctcc ttttatcatg gaattgcccg
     2161 catatcattt tcctcaatgg tcagcggtcc taagacaaac ctatgaccgc agcaagtctt
     2221 ttgttaaaaa agcaggaacg attatctttg tttctagtat cgtgatctgg ttcagctctt
     2281 cttataattt ttatgctcaa cctgtttctg aagaccaaag tatcttagcc tttttcgggc
     2341 gaatcctggc agttgttttt cagccgcttg gttggggaaa ctggcaagga acagtcgcag
     2401 cgattacagg attggtcgct aaagaaaata ttattggcac atttggtatt ttgtttggtc
     2461 atgcggaagt atccgaaaat ggcagagaaa tctggcaagt gctacaacac acatatactc
     2521 ccgcagcagc ttattcattg ttagccttca atttactttg tgcgccttgt tttgcggcaa
     2581 ttggggctat ccatcgggaa atgaatgcca agaaatggac ttggatcgcc ataggctatc
     2641 aatgcggttt agcttattta gttagttttg tgatttatca gttgggacac ttattggagg
     2701 gtggacaggt agcgttaggg acgtatcttg ctattttact gatgatagga ctcgtttatg
     2761 ttttgttccg tcagccaaaa tccaagaacc agttttatac tatattgtca ttagaaggag
     2821 aggaatagga tggcgacatt cttattaagt gtgttaatct ttggaagtgc aggcgcaatt
     2881 gtctatactc gtttaaaatc cggtaagaat tgtgatgact gtcaaacagc ttgcccagta
     2941 aaaaaggaac agatcaagcg atgatcagtc gttgcaagag gctattctcc ccttttttgc
     3001 ttattttgct agagcatagt aagatgaggg aaagtaaaaa aagaaagagg aaagaaaatg
     3061 tctggaaaga aaaaaatcgt ccgcccgcgc taccagcaga ttgctgttga tttagccgaa
     3121 cgtatcgttg aaaaccgata taaagtgggt gagaagatcc atgcccgctc aacacttgcc
     3181 agtacgtaca atgcttctcc tgagactacc cgcaaggcta tcaatgtgct ggtagatctt
     3241 ggaatcatgg aagtacggca tggaagtggg gcatttgttg cttcgaagga aaaagcaaaa
     3301 gatttcctat tgcaatatca agatgtacaa tccatccaag atacaaaagc agaaatcatg
     3361 gcaagtgtgg agaagcaaca agatgagctg aatcattttt ctcaattatt ggatcagctg
     3421 gtcaatcaaa cgaagcgaat acataagatg aatcctttac ttccgtttga attatcttta
     3481 tcagaaaaag caatgcatct ggaccgttca attagcgagt tgaatatctg gcaggcgact
     3541 ggcgccacag ttatcgcaat ttctcataat gaagaactgc ttgtttcacc tggcccctat
     3601 gctaaattta cggcagggga catcgttcat tttattggaa atgaatatac actacagcgt
     3661 atgacaaact ttttttaccc ggagcatcag cttgcagaat gattgtgtga aatattaaaa
     3721 aaatattaac ttggctaaac cttttattta caaagattta gccaagtttt tttcagtttg
     3781 gacaagcaaa tttctttcat ttttgacaaa gtgactacat gatgttaaca tagtgtggtc
     3841 acttttttca gaaagaaaaa tgaatttaca taaaaaagga gaatttgttt ggtaaaagta
     3901 gaagtaaaac atttaacaaa gatatttggt aaaaagacac aagccgcttt agatatgatg
     3961 aatgatcacc agccgaaaac ggagatttta aagaaaaccg gtgcaacggt tggtgtgtat
     4021 gacgtgaatt ttgacgtaaa agaaggagaa atcttcgtga tcatggggct ttccgggagt
     4081 gggaaatcca cattgatccg tctattgaat cgtctgattg agccaacttc gggcagtatc
     4141 tatattgatg gagaagatgt atcgaagtta agcaaagaag aattgcgaga agtccgtcgt
     4201 cataaaatca atatggtttt ccaaaacttc ggtctgtttc cacatcgaac gatcttggaa
     4261 aatacagaat acgggttaga agtccgtggt gtacctaaag aagaacgaca agaaaaagca
     4321 gaaaaagcat tagaaaattc tagtttgctt tcatttaaag accaatatcc tagtcagtta
     4381 tctggtggga tgcaacaacg ggttggccta gccagagctt tagcaaatga tcctgagatc
     4441 ttgctgatgg acgaagcgtt ctcggctctt gatccgctga tccggcgaga aatgcaagac
     4501 gaattactgg acctgcaagc caatgttcaa aaaacgatta ttttcattac ccatgatttg
     4561 aatgaagctt tacggattgg ggacagaatc gccttgatga aagacggcga aatcatgcag
     4621 atcggaacag gtgaagaaat attgaccaat ccagcaaatg attatgtacg tgaatttgtg
     4681 gaagaagtcg atcgttccaa agtgcttacg gctcaaaaca tcatggtacc tgcattgacg
     4741 acgaatatcg aatctgatgg accaaacgtt gcattgacaa ggatgcgtaa tgaagaagtc
     4801 agcatgctga tggctgttga cagaaagaga cacctgaaag ggatcatcac tgcagatcaa
     4861 gcgttagaag ctaggaagca aaaacgtcca ttgatcgact tcctagatga aaatgtgacg
     4921 gtgattggga aagacatggt ggtaagtgat attttcaata ttatttatga ctcaccgact
     4981 ccacttgcag ttgtagagga tggaaaatta aaaggtgtcg tcatccgagg tagtgtgatt
     5041 gaagcattag cagaaacaag cgaggtgagt gaacatgaat aatctattga actatcaatt
     5101 accagtggca aactgggtag aaaatatcac agaatggttt acgacaactt tttctggcct
     5161 attttcattt ttacaaacga ttggacaggc agtgatgtcg gggattacaa atttactgtt
     5221 agtgattcca gctcctttgt ttatcttgtt attgacggta gcagctttct ttatttctaa
     5281 gaaacgtcca ggacttacgt tgttcacatt aatcggtcta tggtttattt ataaccaagg
     5341 tctatggaat gatttgatga atactgtcac acttgttctt ttatctagtg tgatctcgat
     5401 catcattggg gtaccattag ggatcttgat ggcaaaaagc agtaaagccc aaagtattat
     5461 caaaccgatc cttgacttta tgcagacaat gccaggattc gtttatttga ttccagcagt
     5521 tgcatttttc ggtatcggga tggttccagg ggtatttgcc tcagtcatct ttgctctgcc
     5581 accaaccgtt cgttttacga atttagggat tcgacaagtt cctaaagaat tagtagaagc
     5641 ttctgattca ttcgggagca caagtcgtca aaaactattc aaactagaat tgcctttagc
     5701 taaaagtacg atcatggcag ggatcaatca aacaacaatg ctgtcacttt caatggtcgt
     5761 tattgcctct atgatcggtg cacccggctt aggacgcggc gtattatcag cattgcaaag
     5821 agcacaagtc gggaatggat ttgtcaacgg ggttgcatta gttatcttag caatcatcat
     5881 tgaccgtttt acacagcatt tgaaccagcc aaacaataaa aaagctgcag gagcaacaac
     5941 acagaaaagt aaaaaacgtc aaggactgat cattggtgct gtagtagtag tgatcttagg
     6001 tgcgattggt atagggagtt tttcatctgc caaagaaaca aaacgtatca acttatctta
     6061 tgttgaatgg gatacagaag ttgcttcaac aaatgtagtc ggtgaagtat tgaaacaaat
     6121 gggctatgat gtaacgatga cgccattaga taattcaatc atgtggaaat ctgtttcaaa
     6181 tggcgagtca gatgccatgg tttcagcttg gctacctaaa actcatggtt cacaatatgc
     6241 acaatataaa gatcaagtcg aagacctagg tgccaatttg acaggagcaa aagttggttt
     6301 agcagtgcca gcctacatgg atgtaaattc gatcgatgag ttaaccgatc aggcaggtaa
     6361 gaaaatcata ggaatcgagc caggagctgg tgtagttacg gctgctgaaa acacgatcca
     6421 aaaatatgac aacttgaaag actggaaagt agagacatct tcttcaggag caatgaccgt
     6481 tgcactaggt caagcgatca aaaaacacga accaattgta gttacaggat ggacacctca
     6541 ttggatgttt gccaaatatg atctgaaata cttagaagat cctgaaaacg gaatgggaag
     6601 cgaagagcag atccatacga tggttcgaaa aggattgaaa gaagaccagc cagaagctta
     6661 caaagtatta gacaatttcc attggtcaga aaaagatatg gaaaaagtta tgttggaaat
     6721 caacaatgga aaagatccac agcaagcagc aaaagactgg atcaaagaga accaagaatt
     6781 agtcgaaagc tggaaaaaat aaaaagacag acacgaaata aatggagctt ccaaaattgg
     6841 atcaaatatc caaatttgga agctccatgt tcgtctctat cttttttgtc tataaaaaaa
     6901 gagaatgcgt agctcatctt cgaaacgttc aaagacgccg acaaactatt atgaatttat
     6961 gggtgcgagt caaagagatc gatcgtaggt atgaaagact aatgtgatta aagtttatta
     7021 tgtacgaagt gcattctcaa aaaatattaa agaatataca tagtttatat acttaaaagt
     7081 gattaaattc ttattcagga attattttgc tacgtttcaa gaaggaattg ttttttgaaa
     7141 tttaatgaat gttagaataa tagtctaaaa acattaattt aggggtgatt agattatgaa
     7201 acatttaaaa attttgtcta ttaaagagac acaacttatc tatgggggta ccactcatag
     7261 tggaaaatat tatggaaatg gagtgtattg cactaaaaat aaatgtacgg tcgattgggc
     7321 caaggcaact acttgtattg caggaatgtc tataggtggt tttttaggtg gagcaattcc
     7381 agggaagtgc taaaatgaaa aaaaatgcta agcaaattgt tcatgaatta tataatgata
     7441 tatctataag taaagatcct aaatattctg atattcttga ggttttacaa aaggtatatt
     7501 taaaattaga aaaacaaaaa tatgaattag atcccagtcc tttaataaat agattggtga
     7561 attatctata ttttactgct tatactaata aaataagatt cactgaatat caagaggaat
     7621 taataagaaa tttgagtgaa attggaagaa ctgctggaat aaatggttta tatcgagcag
     7681 attatggaga taaatctcaa ttttaatgat acttataata ttaaatatgt ctcaaaattt
     7741 tttgattaga aaaataggta tattgaatca gtgacataaa agtatttata gtttgtaggg
     7801 ggaatcgtaa tggaagaaaa aaatagacta aatgctaaac aatgttcaga tcaagagctt
     7861 aaaaaaatta aaggaggtgc tggaacaaaa cctcaaggaa aaccggcatc aaatttagtg
     7921 gaatgtgttt ttt
//
LOCUS       DAZUMK010000120         7883 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000120 DAZUMK010000000
VERSION     DAZUMK010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.122"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(100..480)
                     /gene="yajC"
                     /locus_tag="ACG808_002399"
     CDS             complement(100..480)
                     /gene="yajC"
                     /locus_tag="ACG808_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFS5047998.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(577..1722)
                     /gene="tgt"
                     /locus_tag="ACG808_002400"
     CDS             complement(577..1722)
                     /gene="tgt"
                     /locus_tag="ACG808_002400"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFS5047999.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1940..2638
                     /locus_tag="ACG808_002401"
     CDS             1940..2638
                     /locus_tag="ACG808_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFS5048000.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2806..5445)
                     /locus_tag="ACG808_002402"
     CDS             complement(2806..5445)
                     /locus_tag="ACG808_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS5048001.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6064..7095)
                     /gene="queA"
                     /locus_tag="ACG808_002403"
     CDS             complement(6064..7095)
                     /gene="queA"
                     /locus_tag="ACG808_002403"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFS5048002.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7288..>7883
                     /locus_tag="ACG808_002404"
     CDS             7288..>7883
                     /locus_tag="ACG808_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5048003.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEA"
BASE COUNT         2227 a         1711 c         1454 g         2491 t
ORIGIN      
        1 tgagcaatat atcccatgct cttatctctt tcttttttga ataaaaaatg aggtaggaca
       61 tctttctgcc ctacctcgtc tttaaataag ctttgatact tatttttctt ccttctcttc
      121 tgtctcttcc agcgtttctg ttgtttcagt ttttacttca actgtctcag ctggttcttc
      181 tgttgtttca acagtagtcg ttgcatcatt tgttacgaca gtccctggtt tgaccgtttt
      241 gattgctgca cgatcgtact caaggaagat cccttcgcag tctaaaacaa ctgtccgttt
      301 gtcattgtcg atttctgaaa caactccatg taatccgccg atcgttacga ctgaatcacc
      361 tggtttcatg ctatctaaca agttttgacg ttcttgctgt tgtttctttt gcgaacgtga
      421 cataaaaaac cacattgcta atagcgcgat aaacatcaca agcattggta atgttcccat
      481 ttttttcacc tcactaattc tgtatctaca aaaaacactt tagcagaaaa atgctgataa
      541 cactagtttt ttacttaatt tttaaaagat tttcctttag aagctttttg cgttttcttt
      601 attgaacccg tattcttcaa aaaaggcttc tctaaattca agcaaattat catccatgat
      661 tgcttgacgt acttgtttca ttagattcaa caagaagtat aaattgtggt aagaagtcaa
      721 acgaatccca aatgtttcat cacatttgat caaatggcgg atataggcac gtgtatagtt
      781 tttacatgta taacaatcac atttctcatc aattggacgg aaatcatggg catatttcgc
      841 atttttcact actagacggc cttgagaagt catacatgtg ccatttcgag cgattcgagt
      901 aggcaagaca cagtcaaaca tgtctactcc acggataaca ccatcgatca atgaatcagc
      961 agcaccgaca cccatcaagt aacgtggttt gttttcgggg atcaacggtg tagtgaattc
     1021 taatacacga ttcatttctg atttaggttc cccgacagat aacccgccga ttgagtaacc
     1081 agggaaatcc atcccgacta gatctttcgc gctttgacga cgaagatctt caaaccctgc
     1141 tccttggatg attccaaata atccttgacg gtctggatta gcatgagctt tcaatcctcg
     1201 ttctgcccat cgagacgtac gttctactga ttttttcacg tagtcgtaac tttcatcgaa
     1261 tggtggacat tcgtcaaaac tcatcatgat atcagagccc aatttatttt ggatgttgat
     1321 tgctttttct ggtgataaaa acatacgcga accgttcaag tgatttttga aatgcacgcc
     1381 ttcttcttcg atattccgca tatcacttaa tgaaaaaact tgaaagccgc ctgaatctgt
     1441 taaaatcggt tgatcccagt tcataaattt atgcagtccg ccagcttctt ctactaagtc
     1501 ttcaccaggg cgtagccata aatgatatgt attacttaaa ataacgccag cacccatttc
     1561 tttcaactct tctggggaca ttgtttttac tgtagctaat gttcctactg gcatgaacat
     1621 tggtgtgggg aaagttccgt gtggcgtgat caattcccct aaacgcgcgc cagtatgttt
     1681 ttctttcttg attaaacgat agcgaatggc aggttgcgtc attcatttcc ctactttcta
     1741 tataaaatat cttgacaact cccaccatca taatcgtttc aagtgctttt tgcaagttcc
     1801 ctcggcttcc ttctacttta tttaatattc ttttttttat ccttttttta tgaataacaa
     1861 aaaaaagaaa aacattatga cagaaatatt ttttgataca tggtatattg gtgttattaa
     1921 agaaaaggag ggaaaaatca tgcaatcttc tgcagaatta aaacgccaag caaaagagag
     1981 tctaaaagga cgttggggac aagctgtttt actaaatcta atccctacat tgattggtat
     2041 tgctactatt cttcttatta tgattcctat tatcttgttt gccaccacca tatatgatgg
     2101 atcagcgagc gtctcagaag cgacaacttc aggaggcggt agttcaggag gcggtggcgg
     2161 aattatttcc agcatcatta gcgctttgtt catgagcggt atttcttgga cttatcttga
     2221 tattttacgt ggacaaaaag atcagatcga accattcaaa gatgcatttc gtggattcaa
     2281 aggtttgttt atcggcggag tgatcagctt agctgtgctg atctcgatct ttacaacatt
     2341 atggggtttg ctattagtta ttcctggaat cattaaaagc tattcttatt ctcaaagcta
     2401 ttttatttac tacgatatcg tccaagaaac aggacaaaca cctaaagtac tggatacgat
     2461 cactgcaagt cgaaaattga tggatggtta taaaggacaa cttttctggt tagatctttc
     2521 atttatcggc tggcatatct tagcattagc aactgctggt attggttatc tatggctcag
     2581 cccatacatt tctgcaacaa aagcagcatt ttatgaacaa ttgccaaaag atatttaaaa
     2641 gagagtgtga cttgtgaccg agggggtggc aggagaacac cgtttatcga gaggcagtaa
     2701 aagaaacatt cagcccatta gcaaaaagga ggtgtgacaa cattcatgtc acacctcctt
     2761 tttgtatagt tccagtaaaa agaaaatcaa cttagaaaca gttaatcatt cctcttcttc
     2821 tgcttccggt aatcgatgga acttgatcag ttcattcacc gcgattggta cgatagataa
     2881 aatccctacc aagaaccagt gattcaaact gatgtgtact aggccgaata gttcttgcgt
     2941 aaatggcagc aaagcaacag ccgttgtaat agctaatgcc agtaacgcca tttctaccaa
     3001 agctttattg gtcgctaaat tgactttgaa gatcgattgg ctgctgcgta cattgaacac
     3061 atgcagaatc gatgaataag ctaagatcaa gaatgcaaca gtttgaccga catgctgact
     3121 tgcgtctacc catgggctga tagtatcgac ataactacct aaataataac caaacaaggt
     3181 tacgatggtg aagacccctg catttatggc aattttttgc aataaacctc gagcaaagat
     3241 cccttcattt ttcggaatgg gtggctgatc catgatgccg tgttccgctg gctctcttcc
     3301 taaagcaaat cctggaagtc catcagcaac tacattgaca aacagtagtt gcacagctgt
     3361 aaatggtgct ccccatccta acagcatcgc aatcaaaaca ataaagatct ccgaaatatt
     3421 acagcttagc aaaaagttga tagctttacg aatattctga taaaccgaac gtccttgact
     3481 gacagcgtct acgatcgtcg caaagttgtc atccgtcaaa atcatatctg cggcactttg
     3541 cgccacatct gttcctgtga tccccatggc acacccaacg ttacttgctt tcaacgctgg
     3601 tgcatcattc acaccatccc cagtcatcgc tacgactgca cccgtacgtt gccaagattt
     3661 gacgatccgg atcttgtctt ctggcgttac ccgagcatag acagagagag ctttgacacg
     3721 cgcatctaat tcttcgtctg ataattgatg cagttctgat cctgaaagtg cttctgacgg
     3781 atcattcaag atacctaatt ctttcgctat ggcactggca gtcaccacat gatctccagt
     3841 gatcatgact gttttgatgc cagccttttt cgctcgcttg attgctcctt tactttctgg
     3901 acgtggtgga tcgatcattc cgatcagacc aatcaaacgt aagtcttttt ccagtgcttc
     3961 agaagaaata tcttgcggtt cttcgtcata agtagcataa ccaacagcaa tcactctcag
     4021 tgcccgttta ccaaagcgat cattaacgat cgccgcttgt tctacatcgc ctgaactaaa
     4081 gcgcggagca agaacatcga atgctccttt ggtgattgag atgtattttt cgcccatttg
     4141 gtgaactgtt gtcatcattt ttcgttcaga atcaaaagga agctcgttta ctcgtggata
     4201 tttttcttct aattctgcct ttgtatgata attctcttca acagccctta cgatggctac
     4261 ttctgttgga ttacctttga taacaaggtc gtcatcttct tgctcgaccg tcacatctgt
     4321 acatagtgca gccattttca atatttccat cgcgctgttc gtcattgaat cttcgatatt
     4381 cgttacttca tcttctttgc tccatacccg gcggacgcgc atttcatttt gtgtcagcgt
     4441 tcctgttttg tctgagcaga taacattagc ggttcctagc gtttcaactg ctggcaactg
     4501 tcggatgatc gcatgtttcc tcgccatctt ctgcacaccg tatgcaagtg tcaacgtaac
     4561 aataacagtc aaggtttccg gtacagctgc aacagccaag gaaatcgaag tcatgaacat
     4621 ctcaaggagt ggttctcctt gcagttcacc aatcacaaat actaaagccg cagccgctaa
     4681 agcgatcata ctgattcgtt tgcctagctg attcaatcgg acctgtaaag gtgttttttg
     4741 catagtattt tcgttcaaaa gatccgcgat cttacccatt tctgtagcca tgccaatggc
     4801 agtgacgatc gctttcccac gacctgcagc aaccgtacat cctttaaata ccatattaag
     4861 ttggtcacct aatgactcat cttcttcagc aataaatgct gcatcttttt caactgcttc
     4921 actttctcca gtcaacgccg attcttctac acgtaattgt gatgcttcga tcaaacgggc
     4981 atcggcagaa atagcatcac cggactctaa aacaagaata tcacccacta ccagttgctc
     5041 tgactcaaca gtggtcactt tgccgtctcg tatgacagtt gccgtttgtt tattcatatc
     5101 ttgcaaggat tccagtgctt tttctgcgtt tccttcttgg acaatcgcca ataccgagtt
     5161 gatcacaacg atagcgatga tcagcagtcc ttcaaataaa tccccatgtt ctgtagcaaa
     5221 tgcggtataa aaggaaatca ccgctgctac aagaaggatg atcgttgtaa aatcggacaa
     5281 gcttcgaaag aattttttga tcatcgattc tttcggcgcc tcatcgaatt tattcaatcc
     5341 ctgctttttt cgccgttcat ccacttcttg tgaagacaac ccagtatctg gatctgcttc
     5401 tgttaccttc tttacttctt ctattgtctg cttgtattct tccatcctat tcctccttta
     5461 aaatcttcgt acaaaaaaag gtacttattt tactataaca tccttttttt atttacttca
     5521 aataaagggc ttatcgatta tttgttaaga aagcgtaaaa tttaaagtgc ttatatttgc
     5581 aaacgctacc aaaggatgct attatataga tgtaaggaag gaggatacca tcaaatagac
     5641 agaagaattt ctcgatagaa cagtgtaagc aaacaaagtt gttagaagta caggtttcac
     5701 ccagacatgc gtgtagagta caaacctata aatagtgaat gcgtatggat gtttggatca
     5761 cgaagtatcc gaaacaagaa gtaatgcgat acgtaacaat tgaataacgt aaacgtctga
     5821 aaattgaata ggaacaagag atgtttcaaa tgttgaagat gtaacgaaaa caaaattttt
     5881 gtttaacatg aagaaattca acaccgtgct ttgcatgtat gatggaaagt tacaatcatt
     5941 tctgtttatt tgaatccttc cgaggtacaa gaaagccgag cttatcgaga gctcggcttt
     6001 ttctttgtca taaaatcttt gtaaagcttt tttgttattc aaaactagcg gcttttcata
     6061 accttatttc acgaacatcg catctccaaa actaaagaaa cgatagcgct cttcaatcgc
     6121 atgctggtaa gcagataaag tcaattctct tcctgcaaag gcactaacta acatgactaa
     6181 agtcgatttt ggcaaatgga aattagttga gaaagcttct accactttga attcatagcc
     6241 aggcgtaatg aaaatatcag tccatccgct gtctgctttg atttctccat taaatttcgt
     6301 tccaatcgtt tctaatgttc gaattgaagt tgttccgaca gccacgattt tcccgccatt
     6361 tttacgtact tggttcaatc gctcagccgc ttcttccgtc aaacggtaga attcactatg
     6421 catatggtgt tcttcaattt tatctacgct gacaggtcgg aatgtaccta accctacatg
     6481 tagtgtcaga tagactaatt caacaccctt tgcttcgatc tcatcaagta attctttcgt
     6541 aaaatgcaat ccagctgttg gcgctgcagc agaaccattt tctttcgcat agacagtttg
     6601 atagcgatct gggtcttcca gacgttcttt gatatatgga ggcaatggca tttctcccaa
     6661 tgattcaaga atctctagaa aaattccttc atactcaaag tcaatgatcc gtccgccgtg
     6721 ttccaattct tctttcacta ctgcttgcaa tctgccatca ccaaaagaga ttcttgtccc
     6781 aactttggca cgtttcgcag gtttgatcaa tgtctcccac gtatctcctt gtgtattcgt
     6841 tagaagaagg acttctaaat gcgcaccagt atctggtttt tctccatata aacgagcagg
     6901 cagaactcgt gtattattca taactagcgc atcaccaggc tgtaactctt caatgatttc
     6961 atggaaatgt ttatcttcca tctctccagt tttccgatcc aaaacaagta aacgagaatg
     7021 atcgcggtct tttaatggtg tttgtgcgat taattcttcc ggcaagtcaa aatcaaaatc
     7081 ttctgttgtt aacatatcgt cactcacttt ctatcatgtg cttcatcatt ctatcatgtt
     7141 tttcgttctg ttggcaaaat cagcactttt tcacttctat tttttgtttt cctaaataaa
     7201 aaaactaccc tatttgtatt ttttcattgt tttaggcata ctagtaatag atagttcttc
     7261 attgaaaata gaaaaagagg tggcaccatg attgaatttc aaaatgtgtc aaaaatttat
     7321 aaaggtggaa aagtcgctgt agaggacgtc aatctttcat ttgaaaaggg tgagttcatt
     7381 tgttttatcg gtacgagcgg aagcggaaaa acgacgacga tgcggatgat caaccgaatg
     7441 actgatccaa cacaagggaa aattttgatt gatggaaaat ccatccaaga aattgatcca
     7501 gtggaattgc gacgtcagat cggctatgtt atccaaagta ttggattgat gcctcacatg
     7561 acgatccgtg aaaatatcac tcttgtccaa aaattgttga aagtcagtca agaagaacgg
     7621 aataaaacag ctgaaaaaat gattgatctt gtggaattgc cccgtgagat gttagatcgc
     7681 tacccacatg aattatctgg tggtcagcag cagcgtattg gagtcgttcg tgcccttgca
     7741 gcgaatcaag atatcatctt gatggacgag ccattcggtg cgcttgatcc aatcactcgt
     7801 gattccttgc aagaccttgt caaagattta caagagcgtt tgggaaaaac gatcgtattt
     7861 gtaacccatg acatggatga agc
//
LOCUS       DAZUMK010000121         7629 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000121 DAZUMK010000000
VERSION     DAZUMK010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.123"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(14..712)
                     /locus_tag="ACG808_002405"
                     /pseudo
     CDS             complement(14..712)
                     /locus_tag="ACG808_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            791..967
                     /locus_tag="ACG808_002406"
                     /pseudo
     CDS             791..967
                     /locus_tag="ACG808_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1143..1712)
                     /locus_tag="ACG808_002407"
     CDS             complement(1143..1712)
                     /locus_tag="ACG808_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS5048004.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="ACG808_002408"
     CDS             complement(1786..2109)
                     /locus_tag="ACG808_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048005.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="ACG808_002409"
     CDS             complement(2263..3075)
                     /locus_tag="ACG808_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048006.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3402..4277
                     /locus_tag="ACG808_002410"
     CDS             3402..4277
                     /locus_tag="ACG808_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5048007.1"
                     /translation="MGKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            4291..4614
                     /locus_tag="ACG808_002411"
     CDS             4291..4614
                     /locus_tag="ACG808_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048008.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            4611..5363
                     /locus_tag="ACG808_002412"
     CDS             4611..5363
                     /locus_tag="ACG808_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFS5048009.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="ACG808_002413"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="ACG808_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2272 a         1512 c         1371 g         2474 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acctatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aacgtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtgtgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt aggatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcggcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggttcg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaaa
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag ttacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgagaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgaa tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagctttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaacgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tcgaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aaccttttt
//
LOCUS       DAZUMK010000122         7600 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000122 DAZUMK010000000
VERSION     DAZUMK010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.124"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(138..428)
                     /locus_tag="ACG808_002414"
     CDS             complement(138..428)
                     /locus_tag="ACG808_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048010.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(546..806)
                     /locus_tag="ACG808_002415"
     CDS             complement(546..806)
                     /locus_tag="ACG808_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048011.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(943..1356)
                     /locus_tag="ACG808_002416"
     CDS             complement(943..1356)
                     /locus_tag="ACG808_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFS5048012.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="ACG808_002417"
     CDS             complement(1812..2297)
                     /locus_tag="ACG808_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048013.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="ACG808_002418"
     CDS             complement(2436..2651)
                     /locus_tag="ACG808_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFS5048014.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="ACG808_002419"
     CDS             2875..3675
                     /locus_tag="ACG808_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS5048015.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="ACG808_002420"
     CDS             3694..5562
                     /locus_tag="ACG808_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFS5048016.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="ACG808_002421"
     CDS             5555..6046
                     /locus_tag="ACG808_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFS5048017.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /locus_tag="ACG808_002422"
     CDS             6034..6588
                     /locus_tag="ACG808_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFS5048018.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..>7600
                     /locus_tag="ACG808_002423"
     CDS             6606..>7600
                     /locus_tag="ACG808_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFS5048019.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1213 c         1443 g         2237 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacatagaag aagggatata taaagtaatg aaacatttca agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgtttccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg
//
LOCUS       DAZUMK010000123         7534 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000123 DAZUMK010000000
VERSION     DAZUMK010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.125"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(552..1898)
                     /gene="gor"
                     /locus_tag="ACG808_002424"
     CDS             complement(552..1898)
                     /gene="gor"
                     /locus_tag="ACG808_002424"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFS5048020.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(2032..3285)
                     /locus_tag="ACG808_002425"
     CDS             complement(2032..3285)
                     /locus_tag="ACG808_002425"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFS5048021.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(3291..4214)
                     /locus_tag="ACG808_002426"
     CDS             complement(3291..4214)
                     /locus_tag="ACG808_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFS5048022.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4435..6927)
                     /locus_tag="ACG808_002427"
     CDS             complement(4435..6927)
                     /locus_tag="ACG808_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFS5048023.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6934..7401)
                     /locus_tag="ACG808_002428"
     CDS             complement(6934..7401)
                     /locus_tag="ACG808_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFS5048024.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         2075 a         1638 c         1208 g         2613 t
ORIGIN      
        1 aaatcagtgt tttgtcctga gtattagaga aacaaatgga aaaatagttc ctcatatttt
       61 gccgtttttt gagctaatac cgaaggacag ctgattgaac actatttgtt cggaaaaagt
      121 gactaactat gacacaattt ttttcacagt cccttttttc acttgatttg attcgtacca
      181 gcgaacttga cgagaacttt cttggtttgg taactatatg gtattctttt aacatcctcg
      241 tttgcttctt ctaatttatt tatacaaagt cacacttttt taaacatctg ttcttcgata
      301 catttctatt tcttttaaac atcgatacca atattttgaa cttaagagtg cataccaaat
      361 ggattcattt actcttcgtc agttacgaag ataatcaaaa tctccaactt ccctacttat
      421 ttcgttttga gaattatcga agcaatctta cataaataac taaaaaacaa aaaaccagaa
      481 aatcagtata ctgattttct ggttttacat ggtttgttca ataaaaagct gttggaactg
      541 ttaatattaa attatcgcat cgttacgaat tcttccgctc ctgttggatg gatggctact
      601 gtattgtcaa agtcttcttt agttgcaccc atcttgatag ccacggcaaa accttgcagc
      661 atctcatcga caccaacacc aatagcatgt aagccgacga ttctttcttc ttttccgaca
      721 cagatcaatt tcatttcgca tttttggcga taatcattta atgcaaagta cattggggta
      781 aagcgtgaac gataaacttt caattcattt tcgccatatt tttccaatgc ttcttcttct
      841 gtcaaaccaa ttgttgcaat tggtggatgc gtaaagacga ctgtcggaac aagtttatag
      901 tcaagataag aatctttctt tccattaaat aaacgttctg ataaacgtct accagctgcg
      961 attgcaacag gagtcagatc taatttaccg atcacatcac caacagcaaa aatatgttca
     1021 tccgttgtat tttggtattt atctactttg ataaaccctt ttttgtccaa ttccacattt
     1081 gtattttcca gacctaattt ttcggtattc ggtacacgtc caccggcaaa aagtacacaa
     1141 tcaccagtta aggtttcacc attttcaaat agcaccgtat attcgtcatt gtttttagta
     1201 atttctttag caatatgatg tgcgtaagtt tttattccag cttcttggta catttggatc
     1261 aaattgtccg aaagcatttt atcaaacgtt ctaagtggtc gttcttttcg atatgcccac
     1321 atgacttcac tacctaaacc acgaaataca cctgaaagtt cagccgcaat gtatccagca
     1381 cctaaaacga ttgctctctt aggtaactct gttaattcaa aaaatccatt tgaatcaatt
     1441 gcgtattctc caccaggaat ggtcatttta cttgcacggc ctccagtagc gatcaagatt
     1501 ttaggagcac gataggtttc accgtttact tcgatcgttt gactatcgat aaaagtagcg
     1561 taacctttta tagcctcgac atgattgcta tctaatcctc tttgataagc accgtgtaga
     1621 aaatcaatat atttttctcg tctttctact agaccggcga aatcaacatt ttttaaatct
     1681 gcttggatac cgtaacttga ggcatctcgt tcaattgttt ccaaaatcgt acttgcttgc
     1741 cacataactt ttttaggtac acaaccgacg ttgacacatg ttccgcccaa ttcattcgct
     1801 tcaatcagca aaacttttgc gccatgcatc cctgctctgt ttgctgaagc aataccgcca
     1861 gagccgccac caattacgat gtaatcatat atttgcattt tgttcctcct tattgaagag
     1921 atcaatcaaa acctttattt aacgatcctt tttctacaga aaacaagtgt aacacttttc
     1981 ttgaattttg tatgcgaaga tgcttaaaac agggatatat cagctttttc cttatttacg
     2041 ttttcggatc atatctccag gtttcaccgg ctgtgcgact ggcatagtta aaatcatcat
     2101 tggatttggt gctcggtcaa tggattcgcc ttcttcatta tgcataactt caacggtttg
     2161 actgaaatga ctgaatcctg gaccataaaa ttcgatttca tctcctacag aaaacaagtt
     2221 tctctgacgt atcgtagcaa tctgtgttgc ctcatcataa gataatactt caccgacaaa
     2281 tttatatact gggattttac gacgttcacc gaataattgt tcgttttcag ttggtgtatt
     2341 gtagtaaaaa cctgtagcta attctcgttg tgctactttc cataactcgt cgatccattc
     2401 ttgcttgcag acatagtttt caggatcttc catataactg tctacagccg ctttatagac
     2461 atttgacacc gtagatacat aatggatcga tttcattctt ccttcgatct tgaagctgtc
     2521 tacgccgttt tcaatcaatt ctggaatatg ttcgatcatt gacatatcca ctgcactcat
     2581 cgagaattct tcttccactt cgcctgtatc agttaaactg tttcgttctt gaccaaaagg
     2641 catatcgaac aatccatact tccagcgaca agattgagaa catccgccac ggtttgcatc
     2701 ccgcatcgac atatggttgg acaatgtgca tcgaccagaa taagagatac acatggcacc
     2761 atggataaat gcttcgattt cggtatccgt atttttacga atctctgcca cttcctccat
     2821 tgaaacttca cgagctaaaa cgacacgttc caacccttct tctttccaga attccaatgt
     2881 ctcaaaatta gttgctgaag cttgagtgga taaatggatc ggcagacctg gggcttctgt
     2941 cgcacaaatt tcgatcaaag ctggatcaga aacaatcact gcagaaatac cgacatcacg
     3001 caaactgcgg aaaaattccc ctgctccatc ttcatttcct tcatgggtga ccatattagc
     3061 agcaacgtat accttcgcat catgttcttt tgcaaaagca actccttcag ccatttcttc
     3121 ataagtaaaa ttacctgcac ggcttcttaa accatatgca ttcccgccaa tatatactgc
     3181 atctgctcca taatggatcg cggtttttaa cttttccagt gttcctgctg gcgctaggat
     3241 ttctggtcgt ttcaattttc tttttactgt catttctttc atcatactcc ttatttgata
     3301 gcagaaggat caatataaaa gaatcctgta tctaattcac ggttttcagg atgcaattta
     3361 ctgatttcgc tagtcatttt ttccgctttt tcttgatccc agctaccttc taaaatttgt
     3421 ttttttgctt cggcgaatag tcgagtgatg tttacgaatt tttcgcctgg agtaaataat
     3481 ccgtccaatt tccatgtaca gtaatgatgc tgataaagtt cattcaattc attcgagaga
     3541 ttcagatcat tgctggcaaa aatatgtgtg ccatgactat cttcataaat agaataatgt
     3601 gtctcttctt ttttaggttc cgagataaaa agacctcttt cacgatcttt ttgttcgtct
     3661 tgtttcgtat agttatagta attctgtagc agtggacgtt tggattgatg gatgcatgta
     3721 gcaccataca caagggtttc tacaggaata tcgagttttt cttccatcgc cttcatctct
     3781 tcaaaaggaa cttcacgagc aagtactgct ccaactgcgc cttttttcgc ccagaaattg
     3841 atttgacgtg cgctcgttac taatgtttca gcatcataaa tatacggtat acgcaattca
     3901 ggattttttt tcatcgtata aataatacct gtatcaccaa ctgtgatcac atctacgttt
     3961 atttctttta aaaaagccaa atattcaggt acatttttca ttttttcagg atgaatgatc
     4021 ccatttacag caatattcgc ttttttacca tacttgtgtg ctaattctac caattgtcgc
     4081 tgttcttctc gtgtaaatga agcaggcaga cgcaatccaa attgctcttc accgaaatat
     4141 agtgtatctg cacctgcagc aagcaactgt tctgcttgtt caatagtttc tactgtggtt
     4201 atgagttcga tcataatttt ctcctcctac aacttcataa tagtagcata gctctaaaaa
     4261 ctttccaata cttcttctat ttaaagtcgg atttttgaaa attatttcac aaaaaatcaa
     4321 aataaagttc gatatattct gtccaaatat aaaaagagat tgtgacaaaa atcttgtcac
     4381 aacctcttaa tcaagtctta ctattataaa aattcagcta cacaggccaa gtaactatag
     4441 ggaacctact gtttcctttt taggtccgcg aacattcaat gtgatcttac cttttgaagc
     4501 acctactgtt acgtggtcac ctaaacgaat ttctccgcca agcaaagcct cgctcaatcg
     4561 atcttcaatt tctttttgaa gtgcgcgacg gattggacgt gcaccatatt caggatcaaa
     4621 tccagctttc ccgatcacat cgattgcaga tggtgtgatt ttcacttgaa tatcttgttc
     4681 tttcaatcgt ttgataatcg ctttgctcat gatttggacg attgtatgga tttcatcttt
     4741 actcaatgaa tggaagacga cagtctcatc gattcgattc aagaattcag gtcggaaagc
     4801 tttctttaac tcttctaaga tacgtttttg catggctttg tgatctttag taacatcttg
     4861 gacattgaat ccgacatttt tttcttctcg aatttgtgtc gctccgatat ttgaagtcat
     4921 aatcatgatt gtatttctaa agtccacttt tctaccttta gaatctgtta aatgaccatc
     4981 atccaatact tgcagaagta aattgaaaac gtctggatgt gctttctcca cttcatctaa
     5041 gagaatcact gaatatggtt tttgacgaat tttttctgtc aactgtccac cttcatcata
     5101 ccctacgtat cctggaggag acccgattaa tcggcttgta ctgtattttt ccataaattc
     5161 tgacatatca acacggatca gggcatcttc acttccaaac atgacttctg aaagtgcttt
     5221 ggccaattct gtttttccga caccagttgg tccaaggaac atgaatgaac caattggacg
     5281 atcaggatct ttcaatccgc ttcgtgcacg gcggatcgca cgtgaaactg ctttgacagc
     5341 ctcatcttga ccaacgacac gttcatgcag aagtccttcc agttctaaca agcgttcgct
     5401 ttcttttttc tccaactgct gtagagggac accagtccac tccgatacaa ctgtagcaac
     5461 atcttcttct gttacacgat cagaataacc ttgctcctgt tttgcttcaa ttgcagaaac
     5521 tttttgcagc tttttacgta gtgctttttc ttgtctgcgt aattgagcag cattttcaaa
     5581 gtcttgtttt tggatcgctg cttccttttc ttgtactagt tcatcgattt ctagtttgat
     5641 cacagttgat tttgttaagt gatcggtttg atccaaacgg actttcgctg ctgattcatc
     5701 gatcaaatca attgctttat cgggtagttg acgagaagtg atataacgaa cagaaagatt
     5761 gactgctgct cgtacagctt catctgtgat ctccactcca tggtgctctt catatcttgt
     5821 acgtaatcct tgaagaatca cttctgcttc ttcaggagtt ggctcatcta cttgaatgcg
     5881 agcaaaacgt cgctctaatg cagaatcttt ttcgatatat ttttgatatt catctagggt
     5941 cgttgctccg atcgtttgta attctcctcg agctaacgcc ggtttcaaga tattcgaagc
     6001 atcaattgct ccttctgctc cacctgcacc aattaaggta tgcaattcat caatgaataa
     6061 gattacttga ccatcattat agatttcgtc gatgaccttt ttcattcgat cttcaaattc
     6121 accgcgatat ttcgtaccag caaccaaaga tcccatatca agcatcatca ggcgcttttc
     6181 ttgcatgtct tctggcactt cacctaaaat gattttttga gctaaccctt cagcaatggc
     6241 tgttttgcct acaccaggtt cacccaccag tacagggtta ttctttgtcc gtcggctaag
     6301 aatctggata agccgtttca cttctctcga acgaccaaca actgggtcca gcttgttttc
     6361 ttttgctaat ttcgtcaaat cacgtgctaa tgaatctagc gtaggtgttc cttgcggtgt
     6421 ttgccgttta tcttgtacat ttacatttct acgacgattc attccttttc cagcagaatc
     6481 accagctagc cccatttttt gtttcagaac ttgacgggct tttgaaaggc tgatacctaa
     6541 gttgatcatg attcgagtag ccatgatact ttcatctttt aataagctca tcaaaatatg
     6601 ttctgttcca actttttgag aaccagtttt cttcacttcg ctgctagcta aagcaaaaac
     6661 ttgtttcatt cttggtgaat atggaagaaa gactccttta gggtaagaac ggataccacc
     6721 ataaccaatc agatgttcaa tttcttcata aatttccgtt tcattcaaat ccatttcacg
     6781 taatactttt ccagcaattc cattttgctc gaccactaag gctagtaaaa catgttctga
     6841 actaactgat tgatgtttca accgttttgc ttcttcttgt gcaatttcta atactgcttg
     6901 ggctctttca gtaaaaagtt cttccatcta atctcacttc ccatcttcat aacttaaacg
     6961 ttccagaaat gctcggagaa tccgagcacg tgtatgatct tcatattcat tgaaattcaa
     7021 tgtgtttttt gcaattgcac tcaacatcaa gtttccttct ttttttgtaa ttatttggtc
     7081 ttcataaagt ttttgaataa tagcaaaagc atttttctca ctgattgtat catcaaataa
     7141 ctgatttatc tgctcaagta attgtttttt atctgaaatc cttacttttg caattcgtat
     7201 ataaccacca ccaccacgtt tgctttctac tagataacca cgttggatgg taaaacgggt
     7261 attaatcaca tagttgattt gtgaaggaac acagttaaag aggtctgcca tttcaatcct
     7321 tcgaatctcg atcatttcac tattttccag aattttcttt atatatgatt caattaaatc
     7381 cgaggtattc tgatgtgcca tcttattcat tccctttgac taatattgac cttaattata
     7441 acgaatttga taatagaaag aaagaagaac gctttttgtt aaataaatcc tttagaatca
     7501 acaaaaaaaa atacggaata tactattccg tatc
//
LOCUS       DAZUMK010000124         7158 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000124 DAZUMK010000000
VERSION     DAZUMK010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.126"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(126..374)
                     /locus_tag="ACG808_002429"
     CDS             complement(126..374)
                     /locus_tag="ACG808_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048025.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            373..501
                     /locus_tag="ACG808_002430"
     CDS             373..501
                     /locus_tag="ACG808_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFS5048026.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            558..2252
                     /locus_tag="ACG808_002431"
     CDS             558..2252
                     /locus_tag="ACG808_002431"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFS5048027.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKMTNTLTQ
                     EEEHVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            2319..2525
                     /locus_tag="ACG808_002432"
     CDS             2319..2525
                     /locus_tag="ACG808_002432"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048028.1"
                     /translation="MFLIWLDLLPQMQAFEHRLPRGCEKAALHQVIRAAKLKQLLMFL
                     TWLDLLPQMQAFEHRLPRGYEKVV"
     gene            2903..4036
                     /locus_tag="ACG808_002433"
     CDS             2903..4036
                     /locus_tag="ACG808_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFS5048029.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQNGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYE
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            4067..4387
                     /locus_tag="ACG808_002434"
     CDS             4067..4387
                     /locus_tag="ACG808_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFS5048030.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(4416..4880)
                     /locus_tag="ACG808_002435"
     CDS             complement(4416..4880)
                     /locus_tag="ACG808_002435"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5048031.1"
                     /translation="MVTYNWIKLREHPELVKQSAKWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(5009..6258)
                     /locus_tag="ACG808_002436"
                     /pseudo
     CDS             complement(5009..6258)
                     /locus_tag="ACG808_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            complement(6287..6604)
                     /locus_tag="ACG808_002437"
     CDS             complement(6287..6604)
                     /locus_tag="ACG808_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS5048032.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(6630..6950)
                     /locus_tag="ACG808_002438"
     CDS             complement(6630..6950)
                     /locus_tag="ACG808_002438"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5048033.1"
                     /translation="MEKKTIMLVCSARMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDIINMADYGMMNGEKVLDQAEK
                     LINK"
BASE COUNT         2296 a         1421 c         1316 g         2125 t
ORIGIN      
        1 tctatttaaa agttgttcta atgataacgg ttcgcttaaa tttttctttt tcactctccc
       61 ctttttctcc ctccatccat aaatcgtgga agtatccaaa acaacgtagc aagttaaaat
      121 aaggcttagt ccgtcatcag cttttcaaag aagcactgtc tcggtgcttt ttttgtaccc
      181 aaatttctaa aaatagaacc atacacctcc tcaaatcttt tcttcttacc taccctattt
      241 ttatttacag gaaaatcaga cacttcttct tttttgtcaa aatttccttt ttcttctcgt
      301 cctatcattt ggcattctaa taaaaacgaa ctatccttgg atatagaaag agaaaacaag
      361 gaggcgtttt tcatgagtaa aaaattaatc aaagtcggca tcggtttagg tttgcttgcc
      421 cttggcgctg catatctagg aaaaaagaca ggactattcg aagatgacag tcatttatat
      481 gatgaattcg aatcaatcta gcatcctatt tagttgaatc actacggaca tacttggaag
      541 acattggagg aatcacaatg agaaaaagta aagcaataat gattggtgcc ggtattgcca
      601 atatggcagc ggctgtttat ttgattcaag aaggcaaatg gaaaggggat cagatcacct
      661 tttattcatt ggatgatcac ggttcaaatg atggtgcacc gaccgacact gtagtggatg
      721 aatattggaa taaaaaccat ccattagaaa accaaaaagg ctatattgct cgaggcggtc
      781 gcatgcttaa ttaccgtacc tatgtagatt tgatggatct gttaagtcga ataccttctg
      841 ttactgagcc agggatgact gctgaagaag atacgagaga ttttgatgca aaacaccgaa
      901 cctttgacaa agcacgtttg atggaaggtg gcaaaggaat cattgatggc ggaaaactgg
      961 gattcaataa taaagaccgt attcttctga ctaaactgat tgctatgcct gattcagaag
     1021 aagaaaaact agacaatatc actatcgaag aatatttcaa agatgatcca cacttttttg
     1081 aaacaaactt ctggtttatg tgggaaacaa catttgcttt cagaacacgt agttctgctc
     1141 aagaattgcg tcgctatatg catcaaatga tttatgaatt cactcaaatc gaacatctag
     1201 tgggcgtcaa tcgaacacgt tacaatcaat acgaaagtat tatgcttcct ttgatcgagt
     1261 acttgaaaga acaaggatgc aaaatcattt tgaaccgccg tgtcactgac tgggaattca
     1321 aagaaacacc gatgcaagat gaaatcactg tgactggttt gaaaatgacc aatacactga
     1381 ctcaagaaga agaacacgtt gtggtcgatg aagatacagc agttattttc actaatgggt
     1441 ccattacaga ttcagctact ttaggagatt acgaaacacc agctgtagaa aatatggatt
     1501 atggtgcagc ttctagtctc tggaaaaaag ccagtgaacg tttctacaac ttgggaaatc
     1561 ctgacaaatt ctttgctgat cgagatgcaa gcgaatgggt cagctttacc ctgacgacga
     1621 aaaaccattt gttgttgaat gaaatcaccc gtatcacgac tcaagaacca ggaaatgcct
     1681 tgaattcatt tatttcaacc acaccgatca cgccgttagg ccaaaaagat gtcaatatgt
     1741 ctatcgtcgt gcatcatcaa ccgcatttta cgtctcagaa accaaatgaa acagttattt
     1801 ggggttattt cctttatcca agacgacgtg gcgaatttgt ggataaacag tatattaaaa
     1861 tgaatggaaa agaaatgcta gaagaactga ttggacaact ttcaaaagta gatcctggac
     1921 cagtgaatat tcgtgaaaaa gaaacagaaa ttttcgacag cgtaataaat aatatccctg
     1981 tctatatgcc ttatgcttcc gcgttgttca ataatcgtgc caaaagcgat cgaccaaaag
     2041 tcattcctaa acattcgacc aatctggcct ttacaggaga atttgtcgag cagccttatc
     2101 agatgatctt tactgaacaa agtgctgtac gttctggaga aatcgctgct ttccattttg
     2161 cgggtatccc tatgtcacgt cttgtcaaaa caccgcgtta cgacaaagat attcctactc
     2221 tagctcgtgc cgcaaaaaaa atgtttgaat aatcaagagg ttgtgaaaaa gctgccctgc
     2281 atcaagtaat aagaacaacc aggctaaaac agcttcttat gtttttgatt tggttggact
     2341 tattaccgca gatgcaagct tttgaacacc gtttaccaag aggttgtgaa aaagctgccc
     2401 tgcatcaagt aataagagca gccaagctaa aacagcttct tatgtttttg acttggttgg
     2461 acttattacc gcagatgcaa gcttttgaac accgtttacc aagaggttat gaaaaagtcg
     2521 tttagtttcg agtatgaaga aaaattttgt gaaaatggtt cctcacgttt ttgcataaat
     2581 ttgacttaat accgagaaac tgatcccttc tgtcataacc tctttttatt tatgtctgtt
     2641 tctttttgaa aacaaccatt ttttcaacgt ttactctctt gccaatccaa catcatctaa
     2701 cagatacaac tatttcaatg attaccatta cactaaaaaa tcggaaactc acctctccga
     2761 acacaaatac ataagactat agaatgtttc atttaagaaa aaaagcgttt cattatcaaa
     2821 aaggtaagat ctcctttaaa atgaaattat ctgtagtact aataatgcag acaattttat
     2881 gaatctgaaa ggagcatctc caatgaaagc agtcacttgg caaggaaaac agaaaatgga
     2941 aatcagaaac gtagatgatc caaaaatatt agaaccaaca gatgcaatca tccgtatcac
     3001 agcaacagcc atttgcggct ccgatctgca tctttaccat catggtgaag cagttttgga
     3061 aaaagggtat gttgtaggac acgaaccaat gggaatcgtt gaagaagtgg gatctcaagt
     3121 tcaaacattg aaaaaagggc aacgtgtggt tatccctttt aatattagtt gtggtcattg
     3181 tcatttctgc ctgaatcaca tggaaagcca atgtgatgaa tcaaatccag atcagttata
     3241 tggcgggctt ttcggatttg gcaagttgaa cggtaatcat tggggtggtc aagcggaata
     3301 tctaagagtt ccttatgcag actctacttc atttgttgta ccagataatg atcttcctga
     3361 tgaaaaagtt ttatttctat cagatatttt gcccacagct tattggagcg ttgaaaacgc
     3421 aggagtaaaa aaaggcgata cagttgtgat tctaggttct ggtccagtcg gtctattcgc
     3481 tcaaaaattc gctgttatgg ctggagcaga gcgcgtaatc gcagtagatc cagtacagca
     3541 ccgtctagat aaagcggcta attacaatgg ggttgaaact tatttcttag aagacacggc
     3601 acaagctgga acagaattgt atgaactggc taaaggtggc gcagatgtca ttatcgattg
     3661 tgtaggaatg gacggattag agcctgtcaa agaaaaagcg aaaaatttag taagcttaca
     3721 aaacggcaca atctctccat tacagatggc atcgcaagct gtcaaaaaat ttggaacagt
     3781 ccaagtaact ggtgtttata tgactccagc ttcttcttac ccgctgcaag aattttttat
     3841 gcgggatatc gctgtcaagc atggacaggc accaattatc catctaatgc caaaaattta
     3901 cgaaatgatt gcagaaggac tattcgatcc tacacaaatc attactcatt cgctgccact
     3961 atcagaagca gcacaagcat atgagatctt tgacaaaaaa gaagataaaa atataaaagt
     4021 tgttttgaaa ccttgaattc agtaaaaaat acaggtgatt ttctttatgc ccaaaatcaa
     4081 acgaaatacc catgagatcg aaataggaaa agaagaagat atcgaaatac gaggacgtcg
     4141 cttccgcttc tatttccaaa atcgttacac attgatctca ctggctgtcg atttattaac
     4201 cgggattttc tatatttttg gaagccttgc ttccttaact aaaatccccg atcgatatgg
     4261 cacctatttt tatttggctg gtgctatttt cttgacgatt cgtcctattc tcagaatcgt
     4321 aaggaacatt tttatttacg acgaaaaaaa agcaatgaag agaacaagca aaaaaaaaga
     4381 ggcatgagat ttgaaaaaat ctcatgcctc ttttttcacc ctatcgtatc actttgatac
     4441 agacgagcgg tcgtcccact ttcttccttg accattgtca aaaatgtcca ctcgtatcgt
     4501 tcataaaacg tggtatgatc tgtaattaag taaagttttt cgtacccaag ttgatgcatt
     4561 tcttttctga tttccgctaa tatccgcttg gctatcccct gcttacgctt ttccggttca
     4621 acaaacaggg cacaaagatt tggtgtcaaa tcagggcggt tatggaagtc gttttcaata
     4681 acgcccgctc ctccgacgat ggtttctttt tcatctaaga ccacatacca ttgaggaata
     4741 ctttccttat tagcgattcc agtacgaata ctttcctcat acacaacagc aggaactccc
     4801 cattttgatg aaaaccactt cgcactttgt tttactaatt ctgggtgttc tcttaatttg
     4861 atccagttat atgtaaccaa tgtttaaacc tccgactaaa ataaaacgtt tacaaaaatt
     4921 atacaagtct ttatgaaaat tagcaatgac aacaatagtg atgtaaaaaa aacggaaaac
     4981 aatcacttgt tctccgtttt ttgattaatc aagcatctaa agcttctttt cttctttctt
     5041 ggatcgtagc taccttgacg aacggtagat agatcaaaat agaaagaacc aaattgacag
     5101 cggctagtac accacctgca atactttttg ttgccaatac accgccaata attggtggag
     5161 ttacccatgg aggcataacg gtagctgctg gcactaaacc tgtagaagtc gcaaagtaag
     5221 caacactaac taagaccatt ggtgtcaaaa taaatggaat gaacatgatc gggttcaaca
     5281 cgataggtaa cccaaacatc gttggttcat taatattgaa gacacctggc gcaatcgaca
     5341 agtttgttac aaccatatat ggtttgtttt tacgtcctac taaataaata gctaacagta
     5401 aacctaaagt tgcacctgtt cctcctaagt tgacaaagct gtcaaagaaa ggtttgttca
     5461 cgataaacgg tgcgctatgt ccagctgcga ttgcattaac gtttgcttca atggctggcg
     5521 cattgattgt ttgcatcaaa ggatcgacca tattcgctcc gtgtaaacca aagaaccata
     5581 agaatggtgt gataaatgca agcagtaagg cagatggata agagtttgca agtcccataa
     5641 atggttcttg gaccactgta tagaaagaac ctacgatatc atcaacaccg aatgcagcaa
     5701 aaattgcagc aactaaagca aacgcagaga tagtgatcat tgctggtagt aatgaagcaa
     5761 atgcttttgc aactgccggt ggcacaccat caggcatttt gatgatcaat ttttcatttc
     5821 cgcttaaacg tacaaagatt tctgttgaaa taatcccaac aataatagca ataaacaaac
     5881 cgtttgagct catacctaat gctccgccta aagcgaagaa agagcctaaa gaaatcacac
     5941 ctgcggaaac accgtctttt cggtagccgt tagctaaatg ataagcaaga aagcaatcaa
     6001 gacagaaaga atggcaaatg taccgttcca agcagcagcc ccgaatcctt tccatgattc
     6061 accaccaaaa attgagttca tcaattcttg gaacccggga atcggtaagt tattgatcat
     6121 tactgctaat gctcctaaga tcattaacgg catactcacc ataaatgagt cccgaatcgc
     6181 aactaaatgc cgttgtgcac caatttttga tgctacaggt ataaaatgtt tctccataaa
     6241 tgcaacaaat ttatccataa atcttccctc ttttctttac tgtagattac gcgttcaatc
     6301 gacgatacag atcaatgatc tcttttgcta aatctgtaaa agctatggat gtcatcagat
     6361 gatcttgact gtggactgtc aaaagcgtga cttcaacgtg atttccttgt gcttcttgtg
     6421 tcagcatctc tgtttgagag tgatgtgctt ggactaaaga ttcctctgca tctttgattt
     6481 tttcatctgc taaagtgaaa tcaccttttt tcgcagcagc aatcgcttcc attgcatcgc
     6541 tttttgcatt tccgccaaac atgatcagac ccatgatggc ttctagattt ttttgatctt
     6601 ccatttattg tatcctccga taatttttat tatttgttta ttagtttttc tgcttggtcg
     6661 agtacttttt caccattcat catgccatag tcagccatat tgattatatc taaaggaatg
     6721 cctttaggtg ctagcttttc agcaaattga ttttgcataa agcgtacttg cggtcctaaa
     6781 agtaaaacgt ctacattttt agattctagt tgattatccg catctgaagc agatacagca
     6841 aagatgtctg cttccattcc ttttgcttct gctgcctttt gcatttttgt gactaggaga
     6901 cttgtactca ttcgagctga acatacaagc atgattgttt tcttttccat tttaaacacg
     6961 ttcctttcta tacttacact tgtatataag caagtagcgt gccaactttg aaagcgcttt
     7021 aaaaagttga tatcatcaca tttaaggtct ataccaagtt gcatacacac taaaaaagaa
     7081 agtgcacact aaacacacaa attaattttt attacctttt ttcttttaac gtgcacaaaa
     7141 aaagttgtga cacaaaac
//
LOCUS       DAZUMK010000125         6845 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000125 DAZUMK010000000
VERSION     DAZUMK010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.127"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            15..1169
                     /locus_tag="ACG808_002439"
     CDS             15..1169
                     /locus_tag="ACG808_002439"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFS5048034.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1191..1943)
                     /locus_tag="ACG808_002440"
     CDS             complement(1191..1943)
                     /locus_tag="ACG808_002440"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5048035.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2307..4973)
                     /locus_tag="ACG808_002441"
     CDS             complement(2307..4973)
                     /locus_tag="ACG808_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS5048036.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5314..6765
                     /gene="cls"
                     /locus_tag="ACG808_002442"
     CDS             5314..6765
                     /gene="cls"
                     /locus_tag="ACG808_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFS5048037.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
BASE COUNT         2020 a         1412 c         1247 g         2166 t
ORIGIN      
        1 aaagaggtgt tgaaatgaaa atagggattg atcgtctttc cttttttatt cctaatttat
       61 atttagatat gacagagttg gcagaaagcc gtggggatga tcctgcaaaa taccatatag
      121 ggattggtca agaccaaatg gcagtcaatc gtgcaaatga agacatcatt acactaggag
      181 caaatgctgc cagcaaaatc gtaacagaaa aagaccgtga gctaatcgac atggtcatcg
      241 tcggaacaga atccgggatt gatcattcaa aagcgagtgc ggtaattatc caccatttac
      301 tgaaaatcca atcttttgct cgttcttttg aagtaaaaga ggcttgctac ggtggcaccg
      361 cagctttgca catggcgaaa gaatatgtca aaaatcatcc agaacgaaaa gtactagtca
      421 tagcaagtga tattgctcgt tacggcttgg caagcggtgg tgaagtgacg caaggtgtcg
      481 gtgctgttgc gatgatgatc actcaaaacc cgcgtatttt atcgattgaa gacgacagcg
      541 tatttctgac agaagatatc tatgatttct ggcgtccaga ttatagcgaa tttcctgttg
      601 ttgatggtcc tttatctaat tctacgtata tcgaatcatt ccaaaaagtt tggaatcgac
      661 ataaagaatt gtcgggtcga ggactcgaag attatcaggc gattgctttc catattccgt
      721 atactaagat gggaaaaaag gcattgcaaa gcgtattaga ccaaacagac gaagaaaatc
      781 aggaacgtct tatggctcgc tatgaagaaa gcatccgtta cagccgacga atcggtaatc
      841 tttacactgg ttcattatac ctggggctaa cttctctact ggaaaattcg aaatcactac
      901 agccaggaga tcgcatcggt ctattcagct acggttctgg tgcagtaagt gaatttttta
      961 caggttatct ggaagaaaac tatcaagaat atctcttcgc acaatctcat caagagatgc
     1021 tagattctcg aacacggatc actgttgacg aatatgaaac gattttcagt gagacgcttc
     1081 ctgaacacgg agaatgtgca gaatatactt cagatgtacc tttttcgatt acaaagatcg
     1141 aaaatgatat tcgttactac aaaatataat aaaatcataa aaagaaccct tcacgagtcg
     1201 tgaaaggttc tttttttatc tgcggaccaa aggagcagtc tataggtcag cttagtcgta
     1261 ctaaggattt ctcgtttttc actgaatagt ttgcgaatgt acctggtcac aacaggatct
     1321 tttgcgtacg cgtcatagta tagcttcatc aattcttcag taattgattc ctgtgtaaaa
     1381 taagtaaaat cttttttctt cactttgaat ccttcatcaa ttaagtatgt ttcaaattga
     1441 tccattacaa ctggcgatgt attaggatct tcttctatca ttcctagttg cttttcgata
     1501 ggataaaaaa catcatcgat tgattccact actctgatta tcacgcacca tttgcttgac
     1561 tttttcatca atttttctaa ttgaataaga tgtaccaatg ctggatttgc tgctgtaaaa
     1621 accatttcat atgtctcatt tgattggata aattcttcta acgactgttt gataaaggaa
     1681 acatttggca actcattttt ttctgcttct ttttttgcat tttctagcat tcctgaagaa
     1741 aaatcgatca aatcatagcg attaatttct tttgctaagg aaagaaatct gccagatcct
     1801 ccagcaacgt ctgctgctgt ttcagcaggc aggactttct ccgttttcag ataatttttt
     1861 acatcttcag caatagttac ttgcgattct tgctggattt cataatattc ttcagcaaat
     1921 tcatcccatt ctttttcatt catttgattt cctctaattt tttgttatat gttttcactt
     1981 ttcggaataa atcattctcc tcttttatct attctatctc ttttgatgaa taataaaatg
     2041 attggctttc cgtctatcat aaataaaaaa agggactgtg accaaagtcg tgtcaaagtc
     2101 cctctttccg aataaacagt gtccaatcag atgaccctca gtattagctc aaaatgctga
     2161 ttttacaact tcttccttaa acggtgttct cctgccaact cttcggtcac aagtcacaat
     2221 attcaacaaa catgagaagc tgtttgttga aattgcacct tgtgactcag aattaaacgg
     2281 caggctcaca acctcgttga ttcgttttat ttatttcctt gtactcgtac aatgatcaat
     2341 ttagtgattt ccatcaaaac aattgtcaca agtgcgattc ccatacatgt aaagacttct
     2401 gtcaatccaa atgctgatgg gatagagaag acttctttca gtcctgggat cattgttaag
     2461 cagtaaagcg cgctacatac aactacagct agtaagacac ttctgttaga gaagaaccct
     2521 gcttggatag ctgtttgtgt gtttgaacgt gctgggaatg tttgcaatgt ccgacaccaa
     2581 atcaatgttg aaaatgacat ggctacaccg aattcaggtg aatgctgcat gccaagccat
     2641 tgagcaaaga taacagcaat cccaatcagc acaccacgat acgttacaga aatcaatgtt
     2701 ttgtccgtaa agattcctga atttggatct cttggcgggc ggttcatcgt agcaggttca
     2761 gctttttctg tacctaatgc aattgctggc agtgaatcat tgactaaatt gatgaacaac
     2821 agttgtaatg ctgtaaatgg attggaccag tcagcaacta aggcaaagat gatcgcaatg
     2881 atcgcaccta aatttcctgc aaatagataa gaaatagctt tcttgatatt atcaaacaca
     2941 ttacgtccta cttctactgc gctgacaata gaagcaaagt tatcatccgt caataccatc
     3001 gctgctgcat cttttgccac atctgtaccc gttcccatag caattccgat gtctgcttgt
     3061 ttcaatgctg gtgcgtcatt tacaccatca cctgtcatag ctgagatatg acctttattt
     3121 tgccaagcac gaacgatacg aattttattc tcaggtgaaa cacgtgcata aacagtgact
     3181 ttttctaaaa cttcgtttag ttgatcttct gtcaattcat ctagttccgt cccggtcaaa
     3241 gccaggtttc cttcttcaaa aatcccgatt tgttttgcga tagcaacagc tgttgttttg
     3301 tggtcaccag taatcatgat tgttttgatt cctgcacttc ttgcttctgt taccgcctga
     3361 gtcacttcag cacgtggagg atcgatcata gcaagcaaac caacaaggat caaatcgtct
     3421 tcatcttcaa atgataaatc aaaattatca agtggacggt aagcaaaagc tagtacacgt
     3481 aatgcgcgtg ttgaaaaagc ttcattttgc tcttggaatt ttttcatcaa ttcatccgtc
     3541 atcggaacga cttcaccatt tagcaatact tttttactgc gttggaagac gatatctggt
     3601 ccgcctttaa caaacaagta atttttgtta tcaattgtgt gtccagtaga cattaatttt
     3661 cgatctgagt caaaaggaag ttctgcttgt cttgggtagt tgtttcggat ttcctgataa
     3721 ggctggttta atttttcact aaagtcgata aacgctactt ccgtagggtc gcccaattta
     3781 gtaccgtctt cactgatcga tgcatcattc gctaaaacac tgatttcgat caagcgacgt
     3841 tcgtcagctg tccattttga agggtcattt gtgaaatccc cagtatcgcc attacctaaa
     3901 aagtagtcaa caactgtcat tttgttttgt gtcaatgtac cagtcttatc tgtacagatg
     3961 atacttgtcg aaccaagtgt ttcaactgct ggaagcttcc gaataatggc atgacgtttt
     4021 gccattttat tcgttcccaa agataacacg attgttacga tagactgtaa tgcttctgga
     4081 atagcagcta cggctacggc tacggcaaac atcactgcgc tgacgatatc tgcagtcata
     4141 tctcctgtac cttctccaag ataaatacga aacagttgga tacctaaaat caataaagac
     4201 aatgccaaaa tagccaaact tagtttttta ctgaatttat ccagacctcg ttgtaacgga
     4261 gtgacttgct cagttgttga ttccaaaagg ccagcaactt gtccgatctc cgtattattc
     4321 ccagttgaag tcactaagaa tttaccgcgt ccgtaagtta cgatcgttcc actgaatacc
     4381 atatttgtac gatctccgat tggtaccgta cttgtgatat ccgttacttc tttttctgca
     4441 gaagtcgact ctcctgtcag cataccttca tctactttta atgatcccgc atctagtaaa
     4501 cgtccatcag caggtacata atcgcctgcg tctaacaaca cgatatctcc taccacaagt
     4561 tcgtttgccg gaatcgtttt ttccttacca tcacgaatca ctcgagcatc gggcgctgaa
     4621 aggtttttca acgcttctaa agaaccttct gctttttttg tttggacaac actgattata
     4681 gaattcaaca tcaatactgc aaaaataact aagaattcga tgtagtcacc catgaccatc
     4741 tgaatcagtg cgacaatcag caatacgata accattgcat ctttgaaagt ttctaagaac
     4801 aatttccaag ttggcttctt cggcttttct tttagctggt tcaagccatc tctttctaat
     4861 ttttccttcg cttgcgtggt gctcaatcca tcggttgagc tctgcgtttg ctgcatcaac
     4921 gagtcgatcg tttctttata gaccgcttgt tgtgcttgtt ccttgttttc caaatttcat
     4981 cccccatttt ttgatcttgg ccctagtcct gtctgaaaaa aaagagacct aagacaaata
     5041 cgcatttgtc ttaggtctca ccatttcata caataccaga aaaccatttg gttaacttac
     5101 tgttgatatt atcgcaacaa acgttgccgg ttactcccct aaggagatat tcaactgtac
     5161 taatatccta ccttattaac ctacaaaaag caactacttt agcccgattt acctacattt
     5221 tcatgaaagg agtgaaaagt atattcacct tggtaggctt ctttcttcta tcatgctata
     5281 ctaatgttat tgtatgtaag gaggaacatt tttgtggtat ctagtattat aaccgccctt
     5341 tatctattaa atgcacttat tgctttggtg gctattttga tcaaaccccg agatgtagca
     5401 gccatttggg catggctctt agtattgttt gcccttcctg gggtgggctt tgtattatat
     5461 ttatttttcg gacgtggatt aacggataag aaaaaattct atctccgaca aagtgacttg
     5521 aaagaattag aaaactttca gtcttttaga ggagatacca ttgaacatta cgatcctgac
     5581 atgggcgata aagacaaaca gcaatttgtt gacttcttct cttcattaaa tcgtatgccg
     5641 ctgacaagaa tgaattctgt cactcttctc acagacggac aagagaaatt ggattcactg
     5701 cttcaagatc taaaaaaagc caaacattcg atccatatcg aatattacgc atttgtgaca
     5761 gataatatcg gccagcaagt cttacatgtt ttagaagaaa aagccgcaga aggcgtggaa
     5821 gttcgaatat tatatgatgc atttggctct catggcacaa aagcaaaaga tttcaatcgt
     5881 ctaatcaaaa atggtggaca tgtccataca tttgttacct cacaaagggc attacttcgt
     5941 ttccgattga attaccatga tcaccgaaaa atcgttgtga tcgatggaaa gattagttat
     6001 accggtggtt tcaatattgc caatcaatat gtaaatacaa caaaaaaatt cggctattgg
     6061 cgcgatacgc atatacggat tttcggtgcc gcttcttctt tgctccagct tcgcttctta
     6121 acagactgga acgtctcggt acctgaagaa aaaaaggtcg gctatcattt gaattatttc
     6181 tttaaaaaag cagatcgaga tgaatctaag cttgctgata catccatcca gcttgtttca
     6241 agcggaccga ataacgaaag ggaacaaatc aagctttcat ttatcaaatt gattacttct
     6301 gctaaaaaac gtgtttggat acagacacct taccttgttc ctgatgaaag tgtcattgct
     6361 gctttaaaaa tcgcaactgc ctctggtgta gatgtgaaaa tcatgattcc caacaaaccg
     6421 gatcatcctt ttatttatcg agcaacacaa tattatgctc ggcagctgat caaggaaaat
     6481 gtacaaatcc ttgtctatga gaacggcttc ctccatgcaa aaacattgat aatggatgat
     6541 gaaatctgca tggtaggttc agcaaatcaa gatattcgta gctaccgatt gaattttgaa
     6601 acaagtgctg tcatttacga tcctgagttt ttagaagaac ttgctactca gttcaaagaa
     6661 gatgagacac attgttcatc catgacaact gaaacagtca aggaaatgtc taactggcta
     6721 ttattcaagc aacaaatttc tcgattattt tctccaatcc tataattttt ttattcaagt
     6781 agagaaaaat atactaagat taaagggact gtgacaaaaa tcttgtcaca gtcccttctt
     6841 ccgaa
//
LOCUS       DAZUMK010000126         6831 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000126 DAZUMK010000000
VERSION     DAZUMK010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.128"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..600)
                     /locus_tag="ACG808_002443"
     CDS             complement(<1..600)
                     /locus_tag="ACG808_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5048038.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSIC"
     gene            complement(720..1367)
                     /locus_tag="ACG808_002444"
     CDS             complement(720..1367)
                     /locus_tag="ACG808_002444"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HFS5048039.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(1451..2878)
                     /gene="uxaC"
                     /locus_tag="ACG808_002445"
     CDS             complement(1451..2878)
                     /gene="uxaC"
                     /locus_tag="ACG808_002445"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFS5048040.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(2952..4031)
                     /gene="uxuA"
                     /locus_tag="ACG808_002446"
     CDS             complement(2952..4031)
                     /gene="uxuA"
                     /locus_tag="ACG808_002446"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFS5048041.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(4034..5665)
                     /locus_tag="ACG808_002447"
     CDS             complement(4034..5665)
                     /locus_tag="ACG808_002447"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFS5048042.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            5969..6640
                     /locus_tag="ACG808_002448"
     CDS             5969..6640
                     /locus_tag="ACG808_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5048043.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
BASE COUNT         2204 a         1365 c          933 g         2329 t
ORIGIN      
        1 acatatcgaa aatattcccg caacaattgt aaaagttgat ccaccacgaa ctatttgagc
       61 gcctgaatca tctcttgtat aaataaataa tggtgttaat acaccaatga ccaaacttgc
      121 taaagtagct ccaacatttc ggaatgtcga tagcattgtt ctttcttctg taatagaagt
      181 aatagctgat gccattgatc cataaggaat atttatagat gtgtaaaaaa tactccccca
      241 taatatatac gtaacataca tgtatactac cttaacgctc atagaagctg aaatcataga
      301 agtttgatac attaaaaaac ttgctaaagc gactggacca ctcattctta aaatccacgg
      361 tttaaactta ccctgcttag ttgctttcga tctatcaaca atacgcccca tagttacatc
      421 agtaactgca tcgataaatc ttgctactaa aaataatgtt ccaacggcag ctccgcttat
      481 ccccaatact tttgtataaa agatcattaa aaaagaacta gcaaaaatga aagtaaaatc
      541 gtttccaaaa tctccaaaca tatatcctat tttgtctctt ataccaaatt ttctttccat
      601 acaattctct cccttctgtt gttaagcgtt ttcattctga tgaaagtacg tactatactc
      661 tgaagcgtaa agagtcagta tttatttttt acatttcaga gtattatttg ttttttctcc
      721 taataattct ttatctcata aaacttatct atatatttac tagctactcg acttacttct
      781 tcaaaatcac ctttttcaac agaagccaat aaattcccac ctacaccaac tgcaacaact
      841 cctacttcaa accaatcttc taagttctct atacttactc cgccagtagg cattatattt
      901 acgtgtggta atggagcttg ataggctttt acaatggatg gaccaaaagc agaacctggg
      961 aatagtttaa tgatatctac gccagcagta agtgcctcct ttatctcagt aattgtcata
     1021 caacctggta aataaggaat ttgatataaa ttacaaatct cagctgtttg tcgatcaaaa
     1081 gatggactaa cgatatattc tgccccagcc ataattgcga tacgagcagt aaacgcatct
     1141 aaaaccgtac cggctccgat tattacatct ttttgatcag catatttttt agcaatttct
     1201 ttgattattt cttcagcatt aggaactgta aaagttagtt caatcccttt aattccaccc
     1261 gcaataattg aatttattgc tttaatggcc tcatccttat ttttcccacg tacaaccgca
     1321 atgactccag ccgcttctaa cttacttaaa atttctactt ttttcatatc tatctccttc
     1381 ctttatggat acatactttg tctagctgta ctaacttaca aagaataaat ttaagctttt
     1441 atttcaagta ttattttcta cttccgattt caaaaaaatt aaaataatta tacgcattac
     1501 gataacaaat attctccact atctgactaa gatacatttc atcatctgga attcggcctg
     1561 ctttaaccca ctcgcttagt aattgacata atactcgacg aaaatactca tggcgaggat
     1621 agcttagaaa acttcgagaa tcagtcaaca tacctgtaaa attcgacaac aaactttgtt
     1681 gtgccatcgt agtcaactgt tgacgcattc cttcagctgt atcattgaac caccaagcac
     1741 atcccaattg gattttttga atcatacccc cttggaagct ttgcattgct gttgctagtg
     1801 gaagccaatc agttggattt aatgaatata aaattgtttt tggtaagcac ccactctttt
     1861 cagcggtatc gaacaatgct acaacatttt gtgtcaaact agcttgatcc cccatcgaat
     1921 caccaccgac atctactcca atttgttcct ttaatatact tgaattattt cttaaagcat
     1981 ttaaatgcat ttgcatcgtc caaccatatc ttgtatactc agacatcaaa tgtaattgaa
     2041 tacctgtaac aaattgtgca atttctgata aatggaaatc cgtttttcct gctaaagctt
     2101 ttattaacag attgtcgagt attttttcat ctgctatttc ataaaaataa gtattcaaac
     2161 cgtgatctgc taatcgacag ccaatttgat gaaaatgatc cactcgttgg ctcaatgctt
     2221 gcttcaaccc attaaagtct ttgattggta tagccgctgt ttgactaagt tttaaaatat
     2281 agttcgaaaa gcctgatgat ttgatactcc agaccttatc tggacggaaa gtaggtaata
     2341 ccttgaaaga attatcctgt ttagaaagct cagtatgata ccacaagtca tctgtaggat
     2401 catccgttgt acagatggct ttcacatcca tcatctccaa caattctttc gggcgatagg
     2461 aatccgtttg aattttttcg ttagcacgtc gccaaatttc tggtgcattc ttctcagaaa
     2521 ttgtcaaatc aatatcaaag taacgttgta attccaaatg agaccattca aaaataggat
     2581 tacctggcgc cttctccact gtttttacaa aagctaaaaa tttttcataa tcatcacctg
     2641 gaccactgat caactcttct tctataccat tagctcgcat caatcgccat ttataatgat
     2701 ctccagctcc tttatcattt aaccatacct gagtcaaatt cttgtattgc ttattttcta
     2761 gcaaatatgc tgggtctaaa tgacaatgat agtcgataat tggttggtgt tttgcaaatt
     2821 tatgaaaaag tcttttagct atttcattct ctaataaaaa gttcttatct aaaaacatat
     2881 ttaaacactc ctttccttcc gttagttttt agaagagaaa atttttcctc taaataaact
     2941 cccttatata attatttact cgcccccttt tcaagcgctt cccaaatacc atttaaataa
     3001 actgcaccta gagctcggtc atataatcca taaccaggtc taccggtttc tccccaaatc
     3061 atccgaccat gatctggacg aatatagcca ttaaatccat tatcgtacat tgtttttaat
     3121 atcttataca tatctagaga accatattct gatttatgcg gcgcttcgta aaaatcgagt
     3181 ccattgtcac ttaaatattt gatattacga acatgtgcaa aaggaatccg atcccgttga
     3241 caaaattcag atagaatttc atagacatca ttatctggtc gttctgcaat acttccagta
     3301 cataatgtaa taccattaga aggagaatcc actacattac acaaccaatc taaatcgtca
     3361 cgatttttaa caatacgagg taatccgaaa atggaatatg gtggatcatc tgggtgaact
     3421 gccattttaa tcccaacttc ttcacatgtc gggataattg ctttgagaaa atatgcaaag
     3481 ttctctcgta atttctcttc atcaacttct ttataggctt ctaataattg cttaatttgt
     3541 gataaccgat gaggttccca acccggcaat tcaaaattat tagtagaatt ttctacttct
     3601 ctaataattt cctccggatc agtagggata ttttttgaaa tataggctaa cgtagaagac
     3661 ccatcatcta aacgataatc caaatccgat tttacccaat caaatactgg cataaaatta
     3721 taacagataa cctcaatccc aaattctgcc aaattgcgaa tcgtctgttt ataattctca
     3781 atgtattgat ctcgtgttgg taaaccaatt ttgatatcat catgaacatt aacactctca
     3841 ataactttca aggtaagtcc attggcttcg acttcatctt ttaaagattt aattcgttct
     3901 ctcggccaaa cctctccggc tggtatatcc attaatgttc caacaattcc tttcattgct
     3961 gggatctgac gaatttcttg tagtgagatt ttttcttcat ttgccccata ccatctaaaa
     4021 gtcatttcca tagttacttt cctccaactt ctgttatata tttattgatt gttttacgaa
     4081 ctgcttgagg acctactagc atttcgcaaa agattttttc agttttcttt cctaccccaa
     4141 cttgatataa atctaaacca aagatttctt tattttctaa aatttcctgt accgcacaat
     4201 gaatctttga aggatcttgt tcacctaatt tcaatactga taatttatcc tgcaactcta
     4261 ccaataacgg atcaggactt ggcacaaaat tttccccttg atcatcgact gccagtaaat
     4321 aacgtaacca agcagcaata gcgataggaa taaatgttag attttctgca tttccgtctt
     4381 tttcgacata ctttttaatt gtttcaccat agcgaatcgc tactttttga gaagtatctg
     4441 ttgcaattcg ttgtggtgta tctggaatat agggattagg caaacgttta ttaattactt
     4501 catcaataaa ttgttttgga tcaataatgc ctggattttc aactactggt aaaccttcta
     4561 aataacctat tcctttgatt aaaccaacca aatcatcatc ctgcatctct gctgaaattg
     4621 attgatatcc tagcagacaa cctgttactg ccaaagcagt atgaagtgga ttcaagcttg
     4681 tcgtaacctt catgatgtct gctttatcaa ccgtctctct atctgtcata atcacaccag
     4741 ccttttccaa tttaggccgg ccatttggaa aactatcttc aataactaaa taatgaacta
     4801 cctcagtatt agtgaaagca gcaatattgg tatgtttttt tgtgtgaatg atatctaaat
     4861 cctcgagccc catcatcaat agatcttcct ttacattctc tgaaggattt ggcgtaattc
     4921 gatcaatcat actccaagga aaagtcactt tttctttatc ctttaaataa gcaataaatt
     4981 tttcagaaac atatccttct ttttgccaag cttctgcaat cgtaaaaata ctatcttgaa
     5041 aacgttgacc attctgtgag aaattatcag tagaaaccat cgcaatcggc aactctccag
     5101 cattaaatct gctatataac aaggatgtta caatactcat cgtatgttga ggattgctag
     5161 gtccagattt gatatcagct ttgactaccg aaaagaattg ccctttagaa tcttttaaac
     5221 tgtaaccttt ttcagtaata gtaaacgttg taaactgtaa tttaggatct tcaaaatatt
     5281 taaccactct ttgatagtcc gctatattct tcggattgca atataaacta tcagccgtta
     5341 ctgccaaaag tctttttttg agttcaccat ctccactcat aacaatttcc aaaatgtcat
     5401 tgttatagtc atgataagct ttttccacta cttcttcatc aaatgtttca cacacaacta
     5461 ctcctgtttc taattcattt tgatctgcta agcattgagc aatttctgca tgaaatcctc
     5521 gataaagatt tcctccacca aaatgaagcc atactggatg ttgctggcct tcttgcttaa
     5581 cttctttgac atcaaaacac ggcagtagaa tctgcatttc attgaattct tcgatagaat
     5641 tttgccaatt tttaatcgaa accataatta acctcctcaa actgatatgt tagttttgat
     5701 acattttata ttttaaaacc cattgtaacc gttgtcaata taaaaaggga ataaacttgc
     5761 ttttttctac catatcagaa tgattgtatt ttttattcag aaaaataaaa tactccttct
     5821 ttttggactg attttatcga aacttatttt ttcttttttt catgtattct tttattttta
     5881 taactagtgt attacttcaa aatttgatat tatatggcat gaacagaatt aaaatatcag
     5941 ttaaaataaa agttaaggag cattgtgtat gacaattaaa actagtctcc aaaatcaagc
     6001 ttaccaatct attcgccgac aaattatcta tgctgactta gaacctggaa aaaaagtatc
     6061 agaaaaaaaa ttagaagaaa cactttctat tggtcgtaca ccaatacgtg aagcactgat
     6121 tcaactccga caacaaggtc ttgttgatac aattcctcaa agcggaaatt atatctcatt
     6181 aattgattta gattcggcca aaaatgctcg atttgtcaga gaacatttgg aacgacaaat
     6241 tatgttagag tgttgtgcca aaatgaataa ccaaaaaaaa cggattttag aaactataat
     6301 ttcagaacaa gaaaaagctg ttagtcaaaa aaatgctcgt agtttttttc ataatgataa
     6361 tttattccat aaagcttgtt ttgaaattgc agagcgagaa gaagtatggc aatggttaga
     6421 aaataacaat acacacttag aacgatttcg atggctacga gtaaaaacgg aaggattaaa
     6481 gtgggaaaaa attatggaac aacattatca actatttgag gcattaacaa aacaagatcc
     6541 agaagaagct aactttctaa catcgttgca tcttcattta atgcttaatg agcaaaatat
     6601 tgttttagct actcatccaa aatatttcaa aaatatataa cttttaactc tttttaaatt
     6661 aaatggttgt tctgatcaga aaaatatcgc ttgttttctt attatgtaat atatttcata
     6721 aactattctg cataacaact attgtttgta ctttttacat tggataacta attatgactg
     6781 ctgataactt ttgatattaa actagtatct ccaatacaaa taaaaaatag a
//
LOCUS       DAZUMK010000127         6798 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000127 DAZUMK010000000
VERSION     DAZUMK010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.129"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            31..921
                     /locus_tag="ACG808_002449"
     CDS             31..921
                     /locus_tag="ACG808_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFS5048044.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(994..1818)
                     /gene="nadE"
                     /locus_tag="ACG808_002450"
     CDS             complement(994..1818)
                     /gene="nadE"
                     /locus_tag="ACG808_002450"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFS5048045.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1830..3302)
                     /locus_tag="ACG808_002451"
     CDS             complement(1830..3302)
                     /locus_tag="ACG808_002451"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFS5048046.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(3939..4301)
                     /locus_tag="ACG808_002452"
     CDS             complement(3939..4301)
                     /locus_tag="ACG808_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFS5048047.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            4524..5276
                     /locus_tag="ACG808_002453"
     CDS             4524..5276
                     /locus_tag="ACG808_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS5048048.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(5416..6339)
                     /locus_tag="ACG808_002454"
     CDS             complement(5416..6339)
                     /locus_tag="ACG808_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5048049.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(6354..6626)
                     /locus_tag="ACG808_002455"
     CDS             complement(6354..6626)
                     /locus_tag="ACG808_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048050.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         2079 a         1352 c         1162 g         2205 t
ORIGIN      
        1 ataagaaaaa ccttttaaga ggtgatatat atgcaactac aaataaacga agaatcctta
       61 ccggtatatg aagcattagc tagtaaaaca aggatcaaga tcatccagct actttccaaa
      121 aaaaagatga atgtcaaaga tttggcaaaa gaacttggag tgagcagtgc tatcacgacg
      181 atgcacgtga aaaagctaga ggaagcaaac atcatcaaaa cagaaaaagt aggacaacaa
      241 aaaatttcga gccttcgtgt cgataaaatc gatatcagtt ttccagaaaa aatattcaat
      301 gcttttgata cgaaagaaac atcgatacct attggtcact atacaaatta tgcaattgaa
      361 ccaacatgcg gtttagctac gatccatgat tttatcggga aagtggatga accacgctat
      421 tttatggatc ctcgaaggat ggatgcccgc attctctggt ttaccagcgg atttgtcgag
      481 tatcaggcac ctaatttttt aaatacagag gatacattgg agatgttgga agtttctgtg
      541 gaaatttctt ctgaatttcc tttttcaaat gacaattggc cttcagatat cactttcagc
      601 ctaaatggtg tggagctagg aacatggacg agccctggag attttgctga tatccgcggt
      661 aaatatacgc ctgattggta tccagataat cttaatcaat acggcttgct gaagacgatc
      721 cgaattacca aacacttgac gaatatgaat ggagaaccct tgtcaaacat cacgatcaac
      781 gacatcccta aagagcaaga cacttggcat ttgcgaatcg aagtgaaaga cgatgccaaa
      841 catgtggggg gctgtacttt atttggtaaa ggattcggta attacgatca ggatatcaaa
      901 ttaaaagttt actacagctg atatttttct tttctatttt ataaaacaaa aacagccaat
      961 ccctatgaag ggacggctgt ttttgcttta ttgttatttc cagaaatcat ccaaaacgga
     1021 aattggcaaa tgtcgtttat gttgtgtctt gttccaccat gcttcgattt tttcttgtgc
     1081 ttgagcggga acttcttttc cttctagata atcatcaatt tcattatatg tgacacctag
     1141 tgcgacttca tctgcaatca ttggtttccc atcttcaaga tctgcagtcg gaatttttgt
     1201 gtataatttt tccggtgcat tcaatgcttg aagcaattgt ttcccttgtc gtttgtccaa
     1261 acggaataat ggcaaaatgt cggctccacc atcaccaaat ttcgtgaaga atccagtaat
     1321 gttttctgca gcatgatctg tccctaatac ggctcctgct ctttctccag ctacggcata
     1381 ttgcgtaatc atacgttgac gtgctttgat atttcctttg ttaaaatcgc tgattcccac
     1441 acctgcttcg cttaatactt gtacttgtgc atcaacagct ggtttgatat ttactcgcag
     1501 actaacatcg ggctggataa agtcaagagc agcttttgca tcttcttcat ctgcttgttc
     1561 accataagga agacgaacag cgataaattg atagttttca tcttttgttt cttcacgcat
     1621 ttcttccatc gttagttgcg ccaaacggcc tgctaaagta gaatcttggc cgccgctgat
     1681 acctagtaca aaagttttaa gaaacggata ttttttcaaa tatgctttca taaagtcaat
     1741 acttttacgt atttcttctt ttggatcaat tgttggtttg acacctaatt cattgataat
     1801 ttctgtctgc aggctcattt attctcctcc tatgcttctg tatgaagttc tgctacgctt
     1861 tttttcattc gttccatgat agccatttta tggttccagc attcagtaga cagatcgact
     1921 gggtattttt gcggattcaa gtctcgttta tactcttccc atagtgaatc tagattttct
     1981 tttgtatatt ctttgatttc gtttagtgca ggtaattcgt aaactctttt tccttcgata
     2041 aagatatctt gtaaaactgg gcgagctgta aagtctcgaa cagttttatt gataaacgta
     2101 tgcactggat ggaacatgaa gatagcttct tcttctcgag ggtcttcatc ccataatgtc
     2161 acgtagtctc cttcagattt cccatcaaaa tttcgagtga tgcgccacac ttgttttttc
     2221 cctggagtag ttactttttc tgcattgctt gataacttga tcgtatccat catttttcct
     2281 tcatcatctt cgatcgatac tagcttgaat actgcaccaa gggctggctg atcataggct
     2341 gtgatcaatt ttgtcccaac accccataca tcaattttag ctttttgcat ctttaaattc
     2401 aaaatcgttg cttcatccaa gtcattcgaa gcatagattt ttgcttccgt aaatccggct
     2461 gcatctaatt gttcccgaac acgcttagat atataggcca tatctccact gtcgatgcgt
     2521 acgccttgga agttgatttt gtcacctaat tctttcgcca cgcgaatcgc acttggcaca
     2581 cctgatttca acgtatcata agtgtcgact aagaacacgc agtctttatg ggttttagcg
     2641 taggccataa atgcttcata atcatttcca taagattgga ctaacgaatg agcatgggtc
     2701 ccgctagctg gtatcccaaa tatcttccca gcacgtacat tgcttgttgc atctgctccg
     2761 ccgatatatg ccgctctagt tccccagact gctgcatcca gttcttgtgc ccgtctagtt
     2821 ccaaattcca atagtggatc ttctccgatg acagatttga tacgagctgc ttttgtcgcg
     2881 atcaacgttt ggaaattgac catattcaac agtgctgttt ctactaattg gcagtgcgct
     2941 aaaggccctt ccacttggat taatggttca tttgcaaaaa ctagttctcc ttcacgagca
     3001 gaacgaactg ttgctttaaa ttcaaaattt tgtaaatact ccaaaaaacc ttcaggatag
     3061 acttctaatc cacgcaagta agcaatatcg gaatcattga acgtcagatt ctctaaataa
     3121 ttgactaacc gttccaatcc tgcaaaaacc gcgtatccgt ggttaaaagg catttctcga
     3181 aagtaacatt caaaaacagc atgtaaatca gctcgaccta attcccagta tgtctgcatc
     3241 atattgattt gatatagatc cgtatgcagc gtcaaactgt cgtctgggta agtattattc
     3301 atcttgtatt actcctcttg gcaagttttc atttcctacc cattataaca aaaaaaatta
     3361 ataggaaaag aaatcttcca ttttttctga aaaagcacat gttttacgga tatttcttgc
     3421 taaaaatgaa ctccatctgc taaaactatc ctttcttatt ggtatttaac ctaaacgaga
     3481 aagaatactt attttattca atgtatccga gacacgaaga aattagcgtt ttctattttt
     3541 cttacttgat aaaataagta gattttccta ctacaatcgt agccgttaag ataccgatga
     3601 ttgaacaaat ataaccaaat gtctctacaa cataatcaat ggcaaaagtt tttgaagcaa
     3661 taatacgtct ttcctttttt gcttcttatc cccaaagaaa cggatataac ttattatgta
     3721 ttgaattttt ttggaaaggc gaattatgac aagatatacc ttgcgttgaa aaaactcgcg
     3781 gaaaaaatat cacacgtttt taaaaagata caaagtaaga aagcagctgt caatagaatg
     3841 gaaacaaaaa tggcgagaca tcaatcagtc tcgccttgtt ttttattgta tacattagat
     3901 atggagcagg ttaccataaa gatgattatt agtcgttatt aaatagaatc cttcgtattt
     3961 ttttgttcta tcactacaat atctttattt ggacgtttga attgtattcc ggcacgttct
     4021 agctcctgtt tcactcctac atggactttg acatcttctc gtgtcatttt taaaacgtga
     4081 tgaatcgcat agtcgatttc ttgccttgtg atttttttct tcgtattgat catcacgact
     4141 tgctttttat catatgtgtc taccaatagc agttgtgttc gatctaacga aaataatttg
     4201 taataattaa tagccgtatt cccaaaaaag aattccacta ctttaggata aaatgtctta
     4261 atatttaaat tactattcac aggtgtttcg attagcttca tctcattcct cttttctata
     4321 tagtcgattt agatccaatc ttaagttatg ttaccatctt tcctagcatt aaggaatcgg
     4381 ttacaattga acctttaaag acaaaaggat ttacctttta tcacttaata gtataacata
     4441 atttcgtgag aaaaaaatac aattttttct tgaccatctc gttacgtaaa ggattatgct
     4501 tacatttgtc aggaggaaaa ataatggagt acacaataaa gaaaatgtca gagatatcag
     4561 gagtcagtcc gagaaccttg cgcttttatg atgagattgg actgctaaaa ccagctagga
     4621 tcaattcttc aggttatcgg atttatggca aaaaagaagt cgatcgtctg caacacatct
     4681 tattttatcg aacaatggca ttcaagttgg atgatatcca agaggtctta gacaatccat
     4741 cttttgacca ccagaaagct ttaatcaagc atcagcaaat gttgctggaa aaacgggcac
     4801 aaatcgatac cctcttgaca actgtccaac agacacttga tatgtacgaa ggaggaagaa
     4861 aaatgagtga caaagaaaaa tttgaagggt ttaaacaaca aaagttgaaa gaaaatgaag
     4921 aatcctatgg acaagagatt cgacaaaaat acggaaaaaa aaaggttaca gcatccaatg
     4981 aaaaatggat gaatatggat aaagaaacgt atgaagaaat gcaaactgtt gaaaagcaga
     5041 tgttatttga tctcactctt tacttaaaag aaccagaaga tgaaagctta gcagaccgta
     5101 tcttccaagc tcataagaag tggttctctt acagctggcc tgaatatcag ccaaaagcgc
     5161 ataaaggact aggaatgatg tacgaagccg atgaacggtt cactgcatat tatgacgagc
     5221 gaagtggtca tggtgcagca aaagctttaa atgaaattat ccaaaaaaag gcgtaatctt
     5281 ttacatgatc caacaaaaaa gtacacgtaa aatgacctaa taacaaaaca gcagacaccc
     5341 aagttagatt tttatgctaa cttttggtgt ctgctccatt ctttcacaag ttttttcatc
     5401 ctactttaaa tatattcatc gttgaataaa cgtataaggt attttcactt tatccttttt
     5461 ttctgtaaga aacaattgaa atgccatttc tccgcaagct tccagatggt ggtcgatggt
     5521 tgggaaacca agaagctcgc tgatcagcaa attatcgcgt ccaatcacca ctggtgtctt
     5581 atcttgcaga tagctttgca agatgcctgc tgcaaccata tccgaatttg taaagacacc
     5641 atcaactcca agctctttaa aaaagaagcc tgcttgtttt cctttctctg catctataca
     5701 atcccaaaaa acaagtgatt catcaaaaga tggaaaaagt tgatgacata gctgcaaagt
     5761 gatttttgag ttataactta gcctgccact tcgccccaac gtgatgccca attgctttat
     5821 accttgctgc ttcaaataag taagaccttc tgtaattgat tgttctcgat caataaagac
     5881 acagcttgcc tgttttccct tgatttcttc acaaaaaact agaggtccat atttttgata
     5941 tgccaataaa tcatctagtg tattggcacg agacgtaatg atcaatccat cgaaagcttt
     6001 agctgcaaat tcttcaagat accttcgctc tttttcttta tgatagcccg tcggtaaaag
     6061 tgtcactttg taatcataat caaacgctgc ttcaatgatc ccactgatca actgatcaaa
     6121 atacggatga ttggtataag gcagaatcac tccaatgctt ttcgtttttc cataacttaa
     6181 ctgtctggca tttgcattag ggatataatc gacttctttc atgaccgcaa gtatttcttt
     6241 ccttttttcc tcatctacat atggatgtcc attgattaca cgtgaaatag tagaaactga
     6301 ataacctgtg agctttgcga tatctcgaat gtttgccact gctgcttcgc cacctataca
     6361 aaaagatttt cttgtaaata gtctaccaca gctggaccgc attcaaaaaa ctgatacatt
     6421 tttccttcat gcgaaaaact gatccgctgc ccgccccaat atatatcttt tgtcaaattt
     6481 tcgatttcat tcattggtat ttcttttact atattcctat tcaacccttg aaaactgtac
     6541 aacgttaata cggatccctt gaactcgaca aacgcataaa aataattgtt cgttgtgcca
     6601 tctgttatct ctacataagt attcattgca agccctcctt ttgatacttt gagtatagtg
     6661 tatgaaacgc gtctcatagc aaggagtttg ttttgccaaa gaaaagtttt attcctcttt
     6721 cattattacc aaagtctctt attttaaatt gaatacatta taatgatact gtaaatatat
     6781 ctttattatt agtgaatg
//
LOCUS       DAZUMK010000128         6729 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000128 DAZUMK010000000
VERSION     DAZUMK010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.130"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..460
                     /locus_tag="ACG808_002456"
     CDS             2..460
                     /locus_tag="ACG808_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048051.1"
                     /translation="MHHLFLSKTIPRYSKNKKAEKFCLLLNKFTLIAPILAFIIFSVL
                     LSTTLKGKFMERSSHAMILTFLWLLFTRIYIFLMSLKPPKSISLCLVINGIFLLSLII
                     FITPLDRYVTYLYNPLEYWTYFIGILEGIIFYIGYFPNKNNNFYFYRNKL"
     gene            complement(586..1263)
                     /gene="nth"
                     /locus_tag="ACG808_002457"
     CDS             complement(586..1263)
                     /gene="nth"
                     /locus_tag="ACG808_002457"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFS5048052.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1270..1968)
                     /locus_tag="ACG808_002458"
     CDS             complement(1270..1968)
                     /locus_tag="ACG808_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFS5048053.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2236..3420
                     /locus_tag="ACG808_002459"
     CDS             2236..3420
                     /locus_tag="ACG808_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFS5048054.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3426..3992)
                     /locus_tag="ACG808_002460"
     CDS             complement(3426..3992)
                     /locus_tag="ACG808_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFS5048055.1"
                     /translation="MTSFVNPFFSWFQIGFSYKIFLSLLNRKQKNNIMKEKEGVEMDK
                     RQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHIGKIPDPNVTKLANASYM
                     TRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKIRNRHEQLHQKWVQRDAT
                     VFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(4095..4382)
                     /locus_tag="ACG808_002461"
     CDS             complement(4095..4382)
                     /locus_tag="ACG808_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFS5048056.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4553..5905)
                     /locus_tag="ACG808_002462"
     CDS             complement(4553..5905)
                     /locus_tag="ACG808_002462"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFS5048057.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSDPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQEALGEIETAQ
                     "
     gene            complement(6177..6623)
                     /locus_tag="ACG808_002463"
     CDS             complement(6177..6623)
                     /locus_tag="ACG808_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFS5048058.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
BASE COUNT         1833 a         1326 c         1096 g         2474 t
ORIGIN      
        1 tctgcaccat ctttttttat cgaaaacaat acctcgttat tccaagaata aaaaagctga
       61 aaaattttgt ttattattaa ataaatttac tttaattgct cctatccttg catttattat
      121 tttttctgta cttttaagca ctactcttaa aggaaaattt atggaaagaa gctctcatgc
      181 aatgattctc acttttttat ggttgttgtt caccagaatc tatatttttc tgatgagcct
      241 gaaaccgccc aaatccatta gtttatgttt agttataaat ggtatttttt tattatcact
      301 tatcattttt attacacctt tagatagata cgtcacttat ttatataatc ctttggaata
      361 ctggacttat tttataggta tattggaagg cattattttc tacatcgggt attttcccaa
      421 taaaaataac aacttttatt tttatcgcaa taaattgtaa taagttacca tcctagctac
      481 tgagtatgta catttacttt ttcatgcaaa aaagataaac gaaacagctt ttctccataa
      541 taacaagata attcatacat ttatttgcaa ttaaagaaac cacgtctaca ggcgtggttt
      601 cttttttagc gccttttctt ttagacgcat ccgatttttc ccatcttgac atattgatag
      661 caaaggacat acctcgcact tgggatttct tgccgtgcaa tgatatctac caaaaaaaat
      721 cagtgtatga tgtgtcttta cccataattc ttgtggtact ttccgcatca gtgtttcttc
      781 tacttccatg accgtagcat ctagtttgca gatcctcagg cgttttgaga cacgttccac
      841 gtgggtatct actgcgattg ctggtatccc gaatgcatcc cccaatacga catttgctgt
      901 ctttctgcct acaccaggaa gagacatcaa ttcttctctt gacgttggca cttgcccatc
      961 gaaacgttca atcagctgct gtgcacacgc tttgatgttt tttgctttat ttcgatacaa
     1021 accaatcgtt tttattttaa gaatgatttc atcgattgaa gcttctgcta acgcatcggg
     1081 tgtcggaaaa gaggcaaaca aatctggcgt tgctttattc actgatacat ctgtcgcctg
     1141 ggcacttaag atcactgcaa tcaataactc aaatggatta ttgtgcttga gttctccatg
     1201 tgcttcggga aacattccat acatcgtttc tagagcttcc attgtttttt gtttgcttaa
     1261 catattccgc tactcctcca accaattttt cagtgagact ttcggcaatt cttcttgttg
     1321 agctgactgt tctacttctc tttgaagaag ctgctgtttt cttcgttttt ggtcttcttc
     1381 cacttgttgt tttgtacgta gattttttct ttcccacgat agcaaaatgc ggtcaacgta
     1441 attaaaacta taagcctgat ttaatacggc ttcccgcagt gctagcttca aaatttccgg
     1501 ctgataatgg tcttcttcta gccattgtcc gatccgctgg aactcgatcg aagaaagcgg
     1561 gcgtccaaat tcttgttcaa ataattgata cactgactgg atttcttttt cttgtgtttt
     1621 ttcttcgtat tttttttctt gtgtttttaa atactcacct aatagatcat agatggggta
     1681 aagattatat cgatctgctt ttttaccgtt gacaatcgtc gtttctatct tgataaatcc
     1741 atttgttacg agacgatcca gtatttgaaa aatctgatct tgtttgattc ccatatccat
     1801 agcaatcatc tgcaaatcag ggaaagtatc tccttcaagc tgtgccatat gcaactgtag
     1861 aacaaataaa aattcttcag attgtagacc gatcctatga taatttttaa gtaaaagatt
     1921 agatatgact gtttgtccag cgcttaagta attttctaaa ttcaacatga tttctcctcc
     1981 gctcaatcac agtatacgta aaaaaaacat gggaagcaag aacaggtttc ttttctattt
     2041 acttcactga tcggtcagat ttacaatgtc gtctacttga tttttgtttg ttcatgtttt
     2101 tgatgcgtat cttcactttt cggaataaat aaattaagta gatttcgctg ttccttagaa
     2161 tgttgacaag gaaacaaaat tactatacac tatttttgtt tccaagttaa cattattgca
     2221 taaaggagtt tttacatgta cagtttcaca aataaacatt ctaaagtttt tggatacgtc
     2281 tctgtcttat tcactggatt aggatttact atcgtcagtc ctgtacttcc gtttttaacg
     2341 ctgccttatt ctcactccat acatcagcaa gctttctata tcactttgct catgtctgtt
     2401 tatgctctag ctgccttctt atctgccccc attctcggta gcttaagtga tcattttggg
     2461 cgtcatccga tccttattat cagtttatta ggatcaagta tcggatattt gctttttggt
     2521 ttaggaaatt cgatatggat gttgttcctt ggtcgtatca ttgaaggatt gacggcagga
     2581 gaaatctcca ccttatatgc ttactttgcc gacatcacgg aacctaatga aagaaccaaa
     2641 gtttttggat ggatgggtgc tttagtcggt ataggaacaa cgctgggtcc tattataggg
     2701 ggtctattag ctgagttggg gaattctgtt cctatattta ttggagcact ctttactttc
     2761 ttaaatgccg tttatggcta cacatttatg ctagaaagcc ttccaataaa aaaacgttca
     2821 gtagatttaa gtttctccca tgtaagacct tttcaccaat tacaacaatt attcaagatt
     2881 tcatccgtta ttcctttatt gacagcaggt tttgctgttt ggttggcagc tggctctctt
     2941 caatctatct tttctcaatt ttcgattgac gcctttcagt ggaaggctgg tttggttggt
     3001 ttaagcttct ctttgattgg aatcttggat actgtctcgc aattgttcat tatgcctcga
     3061 ttgctcaaaa aattttcaga acagcagatc acaagaatag ggatgttcag tgaaatcctc
     3121 gcctatctat tcatcacatt atctggaatt cttctactgc cgattttgtt tttatttggg
     3181 attatctgtt atggtttcgg tgattctatt tttactcctg tattcaatgg ccaactatcc
     3241 aattctgtct cagataacca gcaaggtctt gtaatgggcg gtacccaaag tatacaatct
     3301 ctatcgcgcg tcatcggccc gctaatcgca ggacagcttt atgctgcagc accatttctt
     3361 ccgacggtat tcggattttt gcttatgatt tttgcctatt atcactacaa taaaaaatag
     3421 tagcttcatt tgctacttcg tataaattct ctgtatcgac cgataaaacg aaatactgtg
     3481 tcaaattctt cttttgacat attttcaaat acagtagcat ctcgctgtac ccatttttga
     3541 tggagttgct cgtgtctatt ccttattttt tgtccgattg gcgtcaattg aaaatagatt
     3601 tcttttttat tctcttcgct ttgataagtg tttatgatcc ctctagctat cagttttttt
     3661 gttagcttac tgatggctcc tctagtcatg taactagcat tcgctaattt tgtcacgtta
     3721 ggatcaggga ttttcccgat atgttcaatc acttctagtt catttagagt aaatcctgca
     3781 aattcttttt ccatttctgg tttattacga tgaataatct cattagctaa atccagatat
     3841 tcttctatta gcagttgtct tttatccatt tctacacctt ctttttcttt cattatattg
     3901 tttttttgtt tcctattcaa caaagataaa aatattttgt aggaaaaccc aatctgaaac
     3961 caagaaaaaa aggggttgac aaagcttgtc ataacccctt tttctgatta aaagatagta
     4021 aagagacagt catcggtaat tgtgatgatt ttttgtcatc acttaaggat gagcggcctt
     4081 ttcaaaaact gtctttaaag atcatcatca gtttctagca catatggctg accttcatat
     4141 gtgtactctg tattcaatgt gccgtcattt tcatctgagt agatgcgtct actgaaagga
     4201 aaacgatgag aaacattatc tttggcgtca tttgctggca tcgtgtaata ttcctcttgc
     4261 gtttcattca actgagagag tacttgatct gccaactcat caatattgat cgttaatttc
     4321 ttcgaagcac ttttataatc ttctaaaaat gcatcttgtt ggacaaaata ttctttattc
     4381 atcttctttc tcacctttct tttcaaaatc aaataaagct gcttgttctt ttatgtaaca
     4441 gctttatctt tgctgcttat tgtgacaaaa aattcgaaga aagacaacta attgtacttt
     4501 tcacaaaaaa agacagaggg tcgtgacaaa attgtgtcac aacccttttt ctttattgtg
     4561 cagtttcgat ttcgcctagc gcttcttgtg caagtaggtt caagtaattc catgggcggt
     4621 caaaatgtgg ttggaagaag aaatctgaaa gtgcgagatc ttctacagtc attttatttt
     4681 gaacagctaa agacatggta tttgctgatt gtgtaatgtc gtatttggac atgaactgag
     4741 cacctacgat acgattcgtt cctttttcat agactagctc catcattact ttttcagtag
     4801 ttggcatgaa ctctggacga taattatctt caaatacagt cgcttggact tctagatcgt
     4861 tcaaagttgc gctttctttg gtcacacctg tcgaaccgat cgtccagcca aataggtaaa
     4921 gtcctgaagt tccttgagta ccacgatacg ccattttatg atctgtcaag ttatttccca
     4981 caagtaaacc ttgacgaacg gcatttgttg ccaaaggaat atagttttgc gtgccacttg
     5041 gattgtagtt gactactgca gagtctccag ctgcaaaaat gtctggatca cttgtctgca
     5101 tgtattcatt cactttgatt gccccgtttg gcagtgtatc tacttttcct tcgatcagtt
     5161 ttgtatttgg acggaagccc acacataaaa tgaccatatc tgtatcaaat gtatctgttg
     5221 gtgtagctac ttgtttcact tttccttctt catctgtgat aaattcagta acattttcgc
     5281 ctagcgcaag agtaacaccg cggtctgtca attctttttc taagatatct gtgaatggtt
     5341 tatctagata tttattcaag atgcgatcta atccatcaat caatgtcact tgttttccag
     5401 attcgacaaa agcttctact aattcaatac cgatatatcc accaccaaca attactacac
     5461 gttttgcttc tttcgcttgt gcaataattt catttgcttg attgtagttt ttgcatagga
     5521 gtacattttt cgcatcgatt ccactaatcg gtgggataat aggccatgaa ccagttgtca
     5581 tcactaattt atcataagaa accgtttctg tttctcctgt tttcagatct tttgccgtta
     5641 ctgttttatt ttctgtatcg acgtctgtta catcgtgttc cattttaact gttgctccta
     5701 atgaagataa ttcttcagga tttgagtaga acaagcctgc tgggtccttt actactccgc
     5761 cgacataaag tgcgataccg caagataaga aagaaatatt gtcatttctt tcatatactg
     5821 tcacttctgc ttctggatga ttttttaaaa tactttttac agctgcagta cctgcatgag
     5881 tacagccaat tactactact ttcatttatg gtccctccga ttaacatttt gtccttcgac
     5941 aaaactacta taacagaaat aaaacggata caacagttta atgcttgtga attcacaaag
     6001 gtttgtttgt agtttcattc cttttcaagc ataagaaccc aatatctaca agattcttat
     6061 tgtgataata ttcactttga tatctaagat tgtgaataaa agacaaaaaa ataaaaaagc
     6121 tagcgttttt ttacacagct agcttttttt aggtataaca cttatagaaa ctgtttttac
     6181 atttgcgcct tttttgctga ctctttacaa tccggacaga gtccatacaa ttccaaccga
     6241 tgttcattaa tctcaaaatc agtcagtctg ctcgcagcca tttctatatc ttcaagccct
     6301 gggtaatgaa aatcaacaat cttaccgcag ttttcacaaa ttacatgata atgacgcttt
     6361 gaactgaaat caaatcggct agaagcatcg ccgtacttca tttcttgaac aaatccaatc
     6421 gaagtaaata agcgaaggtt attgtaaaca gtcgcaacac tcatatttgg aaaccgatgt
     6481 tcaagtgctt tataaatctc atcagccgtt ggatgggaat gatgttcaat caaatattct
     6541 aaaaccgcgt accgttgagg tgtgattcga atgttctctt tttttaactt ttcgataacc
     6601 tcttcgacta acttattttc catgtaaaca ccccacgttg ttattctcgt cttatcatac
     6661 tatttttaca ttttaaaatc cacttaacat gaaaagaaaa ttcaatttta ttgtatttta
     6721 atgaaaaaa
//
LOCUS       DAZUMK010000129         6707 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000129 DAZUMK010000000
VERSION     DAZUMK010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.131"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..137)
                     /locus_tag="ACG808_002464"
     CDS             complement(<1..137)
                     /locus_tag="ACG808_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HFS5048059.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     L"
     gene            355..1422
                     /gene="ulaG"
                     /locus_tag="ACG808_002465"
     CDS             355..1422
                     /gene="ulaG"
                     /locus_tag="ACG808_002465"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFS5048060.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            1440..1901
                     /locus_tag="ACG808_002466"
     CDS             1440..1901
                     /locus_tag="ACG808_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5048061.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            1921..3405
                     /locus_tag="ACG808_002467"
     CDS             1921..3405
                     /locus_tag="ACG808_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFS5048062.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            3448..3747
                     /locus_tag="ACG808_002468"
     CDS             3448..3747
                     /locus_tag="ACG808_002468"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5048063.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            3762..4400
                     /locus_tag="ACG808_002469"
     CDS             3762..4400
                     /locus_tag="ACG808_002469"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFS5048064.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            4405..5265
                     /locus_tag="ACG808_002470"
     CDS             4405..5265
                     /locus_tag="ACG808_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFS5048065.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            5255..5965
                     /locus_tag="ACG808_002471"
     CDS             5255..5965
                     /locus_tag="ACG808_002471"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFS5048066.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            6197..6631
                     /locus_tag="ACG808_002472"
     CDS             6197..6631
                     /locus_tag="ACG808_002472"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5048067.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
BASE COUNT         2201 a         1056 c         1501 g         1949 t
ORIGIN      
        1 tttaaatctc ttcgtatcgt actaatagat acatcaaggg attcagctaa ttcgaaagtt
       61 gatagttgtt cagtctgctg aagcagttcc aaaatttctt tttgacgttt ttcaatattg
      121 acaagtgagt ttttcatgac ttttgcctcc tgagggacat cttgatccat ggatttattt
      181 gactagttgt tcatttaaac attcatatct gtctttatta tactgaaaca atcattgatt
      241 actgaaagaa aatcatataa aaaacaaacg gtcatataaa agggttttca tggatgctga
      301 tacacattaa tatttacttg taagttggaa caacgatatg gggggagcaa gcaaatgagt
      361 aaaactgtca atgaagtaac aaaagaaaag tggttagtag aaacatttcc tgagtggggc
      421 acatacttga atgaagaaat cgaaacagaa gtagtggatc ctggaacttt tgctatgtgg
      481 tggctgggct gtacgggaat ttggctgaaa agtcatgaag gaacgaatat tttatgtgat
      541 ctatggtgtg ggacgggaaa acgtagtcat gggaatggca tgatgaaaaa aggccatcag
      601 atgatgcgta tgagtggcgt tcaaaatatg cagcctaatt tacgtactca gcctttcgta
      661 atcgatcctt ttgcgataaa aaatgtagac gctttaatcg ttacacatat ccattccgat
      721 catctagata tccatacagc cgcagcagtt catcaaaatt gtcctgaagc aacattcatt
      781 gggccaaaag aagtggttcg cacgtggcaa aaatggggaa ttcctgaaga aaaaacgatt
      841 acagtaaagc caggtgatgc agtcaaaatc aaagacatca atgtagtagc tttagaagcg
      901 tttgatcgga cagcgttggt cacttgtgaa gatccagaag aggtattaaa aggaaaaatg
      961 cctcaggaga tggatcaaat tgcagtaaac tatttattcg aaacatcagg agggaatctt
     1021 tatcatgcag gagattcaca ttactccaat ttatttgcca aacatggtaa tgaacacaaa
     1081 atcgatgtct gtttaggggc ctatggtgaa aacccacgag ggataacaga caaagttact
     1141 tctgttgata tgctgcgcat ggcggagtca ttgaatgcaa aagtagtgat tcctgtccac
     1201 tacgatattt ggtctaattt tatggctgat ccaaaagaga tcactgaaat ttggaaattc
     1261 aaaaaggatc gcctgaagta tggatttaaa ccttatatct ggcaagtagg cgggaaattc
     1321 atctatccga caaacaaaga tgatcttgag tttaattttt atcgaggatt tgaagatgtc
     1381 ttctcgattg aaaatgatac accgtttccg tcgtttttat aagaaagaag gggagtttct
     1441 tgctgaaata cttttttgac catcaattga ttcattaccc agataaagaa atcacggatt
     1501 ggcgtgaagc tatcgccgaa agctgtcttg tattgatcga taaggaaatt attgatgaaa
     1561 cgtatgtgga ggagatcatc cattgtgtag aaaaatacgg tccttatatt gtgatcgcac
     1621 ctcaagtagc aatgccgcat tcttcagaaa aaagtgatgg tgtttttgga acagctattt
     1681 catttaccaa gttgaaacag ccaatccgtt ttgaaagtga aggggagaat gaggagaaaa
     1741 cggctagttt gttttttaca ctcgcagcaa aagatccaga agaacatcta gaaaatatcc
     1801 aaaaattatc cgaattatta atgacagatg gattggttga gcgtctcatc tctacgaact
     1861 ccatcgatga ttaccaagtt gtgatggcag aatataatta attgatagag gagaaaaaag
     1921 atgggggaat ttttgttaag catttggacg tattttgcaa caaatatctt gacgcagcca
     1981 gcatacctga ttggttttat cgtgttatta ggctatattt tgctgaaacg cccgcttcat
     2041 gaatgcatcg ctggattctt aaaggcaacg gtcggttact ttattttgac tgttggctct
     2101 ggtggtttgg tcaataattt tcggccaatc ttggttggac taaaggaacg gttcaatttg
     2161 gatgccatgg ttacagatcc ttattttgga caaaatgctg tggatgctgg attaatggag
     2221 acatttggcc gtacctttgg agatgtgatg attttattac tgattgcatt tgttttaaat
     2281 atcttattag tccgttttca gaaatatacg aaactgcgtt ccgttttcac taccggaaac
     2341 gttcaggttc aacaagcagc taccgctttt tggattttac tgttttgctt tcctgatcta
     2401 ggtaggatac aagtattgat ttttatggga ctgattttag gttgttattg ggcagtagct
     2461 tctaacttaa cagtaggaat cacgcaggaa ttgacagaag gtgccggatt tgcagtggca
     2521 catcagcaaa tgttcggaat ctttatattt gcaaaattag ctgagtggat gaaaaagcgg
     2581 gatgaaaaga aaaatagatc aatcaagcaa gacaaaaaaa ttgaagatat caaattacca
     2641 ggattcttgt cgattttcaa tgagaatatg gttgctactt cgttactcat gctatttttc
     2701 tttgggatca ttctgattgt actaggcaaa gattatttga ttcaagcgca attcatgcaa
     2761 gaagggcaga gtttcttatt ctacattatg acgacttctt taaattttgc ggtatatctg
     2821 gcgatcttgc aattaggtgt tcggacattc gtcgatgaac taactcaatc tttccaagga
     2881 atctcaaaca cgattttacc aggagctgtt ccaggaattg acgtagctgc tacttttggc
     2941 tttggttcgc caaatgcggt aacgattggg ttcttgtttg gtgctttggg acagtttttg
     3001 atgattggtt tactgatttt attgaaatca ccaacgatcg tcatagccgg tttcattccg
     3061 ttgttctttg ataatgccgt tatagcagtt tatgccaata accgaggtgg attcaaagcc
     3121 gcttgcctgt tcccgtttct ttcaggaatc atacaagtag gcggttcagc attattcgct
     3181 acatggattg ggatgtcaac atacggtggt tatatcggga tgttcgactg ggcaacagct
     3241 tggccattgt ttacagtaat catgaaagtc ctaggttatg ctggaattgc cgtagttgtc
     3301 attggtttat tggctattcc gcagttacaa tatcggaaaa atcctgatgg ttattttttg
     3361 attgtagaag actatgacac atatgcagaa aagcagagag tataaaagaa aatctgatta
     3421 gacaacactc ataatggagg gattaagatg agaattttag tatcgtgtgc aaatggatca
     3481 ggaacaagct tgatgatgat gagaagtgtg gaaaaagcaa tgaaagaact aaacgtacca
     3541 attaccaaaa tccatcattg tgccatttca gagggaaaaa gttcagctgg ccaatacgat
     3601 gtagttttta cgccagtcaa ttttttgaat atgtttactc aggcagagaa aaaaggagtt
     3661 acagttatcg gtatcaaaaa tgtgatgtca gtcaaagaaa tcagtgaaaa atttgctgaa
     3721 acaaagtggt accaaaaaaa caagtaaagg aaggaactaa gatgagcagg ccgaatttgc
     3781 aaattgcgtt agatcacaat gacttagaac atgcattagg agatgttatg aaagttggtg
     3841 atgtagtaga tattattgaa gtgggcacaa ttctttgctt acaggaagga caaaaagcga
     3901 ttcgctgtat tcgcagtatg ttcccagata aaaaactagt cgctgatacg aaatgtgcag
     3961 acgctggagg aacagtggcg agtaatgttg caaaagcagg agcagactgg atgacagtca
     4021 tttgttgtgc aacgattcca actatggagg cagcacaaaa agaaataggt gaattgcaag
     4081 tagaactcta cggcaattgg acttttgaac aagccatgga ttggcataac atcggtatcc
     4141 ggcaggtaat ctaccatcaa agccgtgatg cgctgttatc tggtgaaact tggggtgaaa
     4201 aagatttatc gaagatcaaa aaactgattg aactaggctt taatgtttct gtaactggtg
     4261 gattaaatcc acacacatta cacttatttg aagggatcga tgtgtatacc tttatcactg
     4321 gtcgtggtat tacagcagca aatgatccga tgaaggccgc acaaaacttc aaagatgaga
     4381 tcattcgtat ctgggggtaa ggaaatggct caaattggga tttatgaaaa agcgttaccc
     4441 aaagatattt catggaaaga acgtttttcc cttgtaaaag aaatggactt cgattttatc
     4501 gagatgtcga ttgatgagac ggatgagcgg ttagcacgat tagattggtc tgaagaaaag
     4561 atagctgagc tgagagaaga gatgttcagc tccggtgtac gtatccattc gatttgttta
     4621 agtgcgcatc ggcgatttcc gtttggttct gctgatccag aaaagaaaaa agcagcgaaa
     4681 caactaatga aaaaagcaat ccatttggcg cataatctaa gcgtcaaagt aatccaaata
     4741 gctggatacg atgtttatta tgaagagaaa tcaatgaatt ccagagaaga ctttatcgaa
     4801 ggaataaaag aaagcgtaaa ggaagcttct aaatatggca tcattctttc cgtggaaatc
     4861 atggatgatc catttatgaa ctccatttca aaatttttag aaatcaaaga acagattcat
     4921 tcaccttttc tacaagtata tccagaccta ggcaatttgt ccgcatggcc ggaaaataat
     4981 ccggctcgag aacttgagaa agggatcgat tatatcactg ctatccactt aaaagatact
     5041 taccctgtaa caaaagaata ttcaggccag tttcgtgatg ttccttttgg tagcggttgt
     5101 gttgattttc ttggattatt aagacatttg aaacgtctag attatgatgg aacatttctg
     5161 attgaaatgt ggagtgaaaa aagccaagac tttcgaagag aaatccagca ggcaaaagac
     5221 tacttaagtt ctaaattgaa ggaggctggc tatgatgtct tgtgaagaaa aaattgcagc
     5281 aatgaagcaa cgtgtccttg aagcaaatct ggcattgccg cgatacggat tagtcaaatt
     5341 gacttggggc aatgtgagtg aagtggaccg tgagctagga gtcatcgtca ttaagccaag
     5401 cggtgtcaga tatgaatgca tgcaagcaga tcaaatggtg gtaacagatt tgtcgggaaa
     5461 tataatagaa gaagatagct taaaaccatc ttcagatctc cccacacatg ttgttttgta
     5521 tcaaactttt gaagatataa ctgctattac tcatactcat tccacgcact ccgtcatgtg
     5581 ggcgcaagca ggacgcgatt tgccagctta tggcacgact catgctgatg ctttttatgg
     5641 taaggttcct tgtacacgcc aattaacgaa agaagaagta agagaagctt acgaagttca
     5701 tacagggaat gtgattgtcg agacgtttaa ggaaagaaaa ttagatccaa acgaagtccc
     5761 tggagtactt gtctatggac atggaccgtt cacatggggg gattcaccta tgaaagcagt
     5821 agaaaacagt ttgatcttag atgaaatttg tctgatggcc aaagaaaacg agctgatcaa
     5881 ccccaatata tgtgaaatcc cgcaatattt actagataaa cattattttc gcaaacatgg
     5941 aaagtatgca tactacgggc aatagagatg ctagggtaag ggtagaaaat taaaagataa
     6001 cacttgatag agagaaacag aggttgtgaa gaaaatttat cacaaccttt ttatttatca
     6061 aaaagtatgc attctaaaag gaaagaggaa ttttaaggaa tcgagagaat ctttacagga
     6121 gagttatatg tgatagatta aatagtcaaa gaactgtatt attagaggga gaattgtata
     6181 agaaaggaag aaaaaaatga atattgattc ccttatgatc gatgagattt ctgaattata
     6241 taagagcgtt ggttggacga attacacaaa agacactgca aggttagcta gagcatttga
     6301 acaatctgaa tctctgataa agcgtaatgg ggaagggaag attatcggaa tcgttcgttg
     6361 gatcactgat tttgcgacga tcgcatttat tcaagatatc ctcattcatc cgaggtatca
     6421 gcgccaagga ataggcaaag tgcttttgaa tgaagctttg gagaagatca ctagttatgg
     6481 accagtccaa atcgaattat tgacagatga tactgagaaa acaaaaaaat tttatgaatc
     6541 agtaggattt gttcaagtaa aggaaatggg tggtatttct tatgtcatag atactagaag
     6601 aaaaaaggtg ctttttggta atgacaaata acatataata acgtacaaaa gaaaaaattc
     6661 tagcttagtt ctttagaaaa acaagaggat agacttagta tcactca
//
LOCUS       DAZUMK010000130         6679 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000130 DAZUMK010000000
VERSION     DAZUMK010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.132"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            166..2763
                     /gene="adhE"
                     /locus_tag="ACG808_002473"
                     /gene_synonym="adhC"
     CDS             166..2763
                     /gene="adhE"
                     /locus_tag="ACG808_002473"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFS5048068.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            2992..4098
                     /locus_tag="ACG808_002474"
     CDS             2992..4098
                     /locus_tag="ACG808_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS5048069.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            4115..5139
                     /locus_tag="ACG808_002475"
                     /pseudo
     CDS             4115..5139
                     /locus_tag="ACG808_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            5145..5624
                     /locus_tag="ACG808_002476"
     CDS             5145..5624
                     /locus_tag="ACG808_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFS5048070.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            5640..6194
                     /locus_tag="ACG808_002477"
                     /pseudo
     CDS             5640..6194
                     /locus_tag="ACG808_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
     gene            6250..>6679
                     /locus_tag="ACG808_002478"
     CDS             6250..>6679
                     /locus_tag="ACG808_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS5048071.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT         2197 a         1151 c         1475 g         1856 t
ORIGIN      
        1 actttatata tttttctata caaacacctg taaataaagc aattttatat ttaaaaataa
       61 ctgtgaaaaa aaatcacaaa aaagtgttta catagaaaaa taaatattgt atgatataca
      121 tgtgaattat ttaacaaata accatgaccc attaggagga taatgatggc taaagtaata
      181 gaaaaagaaa aaacgaagac aattgacgta caagcaatga ttgatgattt ggcagaaaaa
      241 gcaaatgcag cattgaaaga aatggaaagc tttgatcaag aaaaagtaga ccatatcgtg
      301 catgaaatgg caatggcagc tttggatcaa catatgccat tagcgaagat ggctgtagag
      361 gaaacaggcc gcggtgttta cgaagataaa gcaatcaaaa atatgtatgc gtccgaatat
      421 atttggcata atatcaaaca tgataaaaca gttggtgtga tcaatgaaga tgtccaaaaa
      481 gggttgattg aaattgctga accagtcggt gtggtttgcg gagtaactcc aacaacgaat
      541 ccgacttcca caacgatttt caaatcaatg atcgcgttga aaacaagaaa tccaatcgtt
      601 tttgctttcc atccaagtgc ccaaaaatct tctgcagaag cagcacgagt ggtacgtgat
      661 gctgctatcg ctgccggagc tcctgaaaat tgtatccaat ggatcgaaca tccttcgatc
      721 gaagcgactt ctatgctaat gaaccatcca ggtatcgcga tcgtcttagc gacaggtgga
      781 gctggaatgg tcaagtctgc ttactctaca ggaaaaccag ctttaggtgt aggtccaggt
      841 aacgtaccag cttatattga aaaaacagca aaaattaaac gtgcagtgaa cgatttgatc
      901 gtttcgaaaa catttgataa tggaatgatt tgtgcttctg aacaagctgt gatcgtggat
      961 aaagaaatct atgcagcagt aaaagcagaa ttccaagcac accaagttta tatcgtaaaa
     1021 ccagatgaat tgcaaaaatt agaagatgca gtgatgaacg aaggaaaata tgcggtcaat
     1081 ccatcaatcg ttggacattc tgcgatggaa atcgcaaaat tggcaggaat cagtgtacca
     1141 aaaggaacga aaatgctgat tgctgaattg gaaggcgtag gcccagatta tccgctatca
     1201 agagaaaaac tttctccagt tcttgccatg atcaaagcta acaacacgga ccatgcgttt
     1261 gatttgtgtg aaggtatgtt gaatcttgga ggattaggac atacagcagt tatccattca
     1321 gaaaacgaag agttgcatgt caaattcggt cttcgaatga aagcttgtcg tattttagtg
     1381 aatacaccat ctgcagaagg cggcatcggt gatatctata acgaaatgat tccgtcattg
     1441 actttgggat gcggttctta tggtaagaac tctgtttctc gtaacgtctc tgctgtaaac
     1501 ttaatcaatg tcaaaactgt agcgaaacgg agaaataaca tgcaatggtt caaattaccg
     1561 ccaaaaatct tctttgaaaa gaactctcta ttgtacttag agaaaatgga aaacgttgaa
     1621 cgtgtcatga tcgtttgtga ccctggtatg gtccaattcg gttactgcga tacagttcgt
     1681 gaagtattgg cacgacgtaa aaatgatgtg aagatcgaag tcttctcaga tgttgaaccg
     1741 aacccatcga caaataccgt atacgcagga acgaaactga tggcagactt caagccagat
     1801 acagtaatcg ctcttggagg cggttctgca atggacgcag ctaagggaat gtggatgttc
     1861 tatgaacacc cagacacatc tttctttggt gcaaaacaaa aattcttgga tatccgtaaa
     1921 cggacatata agatcgacaa accagaaaaa acacaattcg tatgtatccc gacaacatca
     1981 gggactggtt ctgaagtaac gccatttgcg gtcatcactg acagtgaaac acatgtgaaa
     2041 tatccgttag cagattatgc attgacgcca gatgtcgcaa tcgtcgatcc tcaattcgtc
     2101 atgtcagttc cagcatctgt cacagcagat accgggatgg atgtgttaac acatgcaatc
     2161 gaatcctatg tttcagtcat ggcttcagat tatacacgtg gattgagcct acaagcaatc
     2221 aagttagtct ttgactattt agaaaaatca gtaaaaacac ctgatatgga atcacgtgaa
     2281 aaaatgcaca acgcttctac aatggctggt atggcgtttg ctaacgcatt cctaggtatt
     2341 tgtcactcag tcgcacataa aatcggcggg gaatacggga tccctcatgg acggacaaat
     2401 gcgattcttt taccgcatat tatccgctat aatgcaaaag atccacaaaa acatgcgatg
     2461 ttccctaaat atgactattt ccgtgcagat acagattacg cagatatcgc aaaattctta
     2521 ggtctgaaag gcgaaacaac agaggaatta gtcgaagctt tagcaacggc tgtttatgaa
     2581 ttaggaaaat ctgtcggcat caacatgagt ctaaaagctc aaggagtcac acaagaaaca
     2641 ctagatacaa ccgtagaccg tatggctgaa ttagcttacg aagaccaatg tacgacagct
     2701 aatccgaaag aaccattgat ctctgaattg aaacagatca tcattgatgc ttacaacgga
     2761 taagcaaaag aaagcgtaga aaaaagataa gaaaatagat gaaaaagaac ttggaaaacg
     2821 ttgtttctcc aagttctttt tttaatttcc aagtaactga aagttacttt tgttatttaa
     2881 catttaggtc aattttagac agtttttgtt caaaaagcga tatttatagt ggacggtatt
     2941 actgctatat taaggatata aaatgaaagc gtttaaaaag gtggcgtaaa gatgattatt
     3001 aaagaagaga tttttgtacc taaagaatta gtgaaagaaa aaattgacct gctcgttgaa
     3061 aacctgacaa cgatcaaaga cgataatggt gaatttttgt tggactttga tggactgaaa
     3121 gtagatgaca aaagctggac agtgtggaat tggccacaag gtgtcggtct atatggtatt
     3181 tataaaaatt accagatgac aaaaaatcct cgcgcttatc aagtggtcaa tgaatggttt
     3241 gaggatcgta tggaagaagg cgctccgccc aaaaacgtca atacaatggc accgctattg
     3301 actatggctt acttgtatga agacacgaaa gattcacgct atttgcctta tttagaacaa
     3361 tgggcagaat gggtcatgaa tgacatgcct cggacaaatg aagacgggtt acagcatgcc
     3421 acatatggac cggaaaataa gaatcagttg tgggacgata cattgatgat gacagtgctt
     3481 cctttggcaa aaatcgggaa actgttgaat cgcccagaat atttagaaga agctcgttac
     3541 cagtttatga tccatatcaa atacttgatg gataaacgaa caggattgtg gtatcacggc
     3601 tggacgtttg aaggaaatca taactatgcc gaagcatttt gggctcgtgg aaactgctgg
     3661 ttgacgattg ctattccaga gatcattgaa atcttagaat tatcgaaaga agatgcattg
     3721 cgcaaattat tgatcgaaac acttgaagca caagtgcgtg cattaaaaac ttatcagtct
     3781 gaaagcggtt tatggcatac gttgttggat gatccttctt cttacgtaga atcttctgca
     3841 actgcaggat ttgcttacgg tattttgaaa gcggtccata aacgttattt accacaagaa
     3901 tataaagaag tcgcttatcg tgctatccaa ggattgcttg aacaaatcga tgaaaaagga
     3961 gaagttcaaa acgtttcaat cgggacagga atgggggaca gtttagattt ttatcggaat
     4021 atcggaatca cagcaatgcc ttatggacag tcgttaacgg tgctttgttt gacagaattg
     4081 ctcgtctctt attgttaaaa gaaaggaaac ccatatgaaa aaaacatcac tgctgtttgc
     4141 agcaagttcg cttagcctag gtctgctagg tctaagcggc tgcggaaata attctagtgt
     4201 ggcaactgtc gcagatgcag acgtaaatga gatagttacc ttgcgctttg cttatgcaag
     4261 caacagccag taattgatgc aatgaatgaa tttgggcgtt tagtaaaaga aaaaacagat
     4321 ggttccgtac agatccagta tttcccagat ggacaactgg gtggagaacg tgaactgatc
     4381 gaattgacac agtcaggagc agtagatttc acaaaagtaa gtgcctcagc gttggaaagt
     4441 ttctctaaag attattcgat tttttccgtt ccttatctat tcaatgatga agaacatttc
     4501 tttaaggtga tggataatga agaaataatg gagccaatct accaatcgac caaaaattta
     4561 ggatttaccg gattgaccta ttatgattct ggtcagcgca gcttttatat ggtagacggt
     4621 ccgatccata cgccggaaga tttgaaggga aagaagatcc gtgtgatgca aagcgaaacc
     4681 gctatcaaaa tggtagagct tttaggcggt tcgcccgtac ctatgggaag tgatgaagtg
     4741 tacacgtctc ttcaatccaa tttgatcaac ggatcagaaa acaatgaatt tgtcttgtat
     4801 acagctggac atggcggggt agctaaatac tattcctatg atgagcatac acgtgtgcca
     4861 gatatcatca tcatgaatga tgccatcaaa gaacagctaa ctgatgaaca ggaaaaagcc
     4921 attgaagaag cagcgaaaga atcaacggtt tttgaaattg aagcttttgc aaaagcaatt
     4981 gaagaagaaa aagctttagc acaagaagaa catggagtcc aattcaatga tgtagataat
     5041 acaccgtttc gagaggcagt tgccccactt catgatatat tcaaaaacga tcctacctac
     5101 aaagaccttt atcaaaagat ccaagagcaa ggagcgtagc tgtaatgaaa atgattttaa
     5161 acaaggtatt agagatcctg ggagctgttt tgcttgttgg gatggtcgct gttgttttat
     5221 ggcaagtatt ttccagaacg gttttaggaa atccaaatac ggtcacggaa gagcttgttc
     5281 gattcggtct tgtctggttt gccatgttgt cttccgcgta tgttgtggga caaaaagcac
     5341 gtttagccgt aacggtttta agtgaaaaat taactggcaa aaaaagtaaa attttagaag
     5401 ttgttgttca aagtttattt ctggtatttg ctgcgatgat catggtgtat ggcggatgga
     5461 atgctgtcac attgacaatg gggcagatct cgccttcttt aagtattcct atgggatatg
     5521 tttatctgtc agtacctgta tcgggggtac tcatcattat ctatagcctg atcaatctaa
     5581 tcgatacatt aagcaacaag ccagcaattc aagaaaaaat ataaaaatgg aggaataaaa
     5641 tggcaataga agccggatta gtgttatttt ttgtatttgt tgtgttgctt gtattaggga
     5701 tgccgattgc tataagtatt gcattctcat cgatgatgac tttattgtta gttataccgt
     5761 ttgatgtttc tgcttttagt tcagctcaaa aaatggtggg aagtatcaat agtttttcgc
     5821 tgatcgctat tccgtttttc gttctctcgg gcattatcat gaataatgga gggatagcta
     5881 agaaactagt ggattttgcg atgttgttcg tcgggcgcgt gccgggggct ttggctcata
     5941 cgaatgtgtt agggaatgct ttatttggtt ccatctctag ctcggccatt gccgcttcta
     6001 ccgcgattgg tggagtgttg atccctcagc aagtggaaga aggatatgac cggaagtttg
     6061 cgacagcggt caatatcgcg tcagctccga caggtatggt cattcctcca agtactgctt
     6121 ttatcatgta ttcacttgtt gctggtggtg cttcgatctc tgctttgttt atgggaggtt
     6181 atctcgtagg cgcttttgat gtgcacccca aaagttagac tagaaatcta attaaaaggg
     6241 gtgctttttt tgcggaaata tacatttgtt tttaagaaaa aagtagtttc agactattta
     6301 aataacgaag gcggctacaa atatcttgca cataaatatc aaataaatcg tacactggtt
     6361 agacattggg taaggattta taactatcat ggttgggaag gcttggttgg aggtggcaaa
     6421 agctacacta caaaatttaa acttgatgtt atagaatata tggaaacaaa tggtctttct
     6481 atccaagaaa ctgctaaaaa atttaatatc ggttcaaata gaactctaag taaatggata
     6541 gagcaatatg aagaaggcgg tgcttcttca cttgagagcc aaaaaagggg cagaaaaatt
     6601 agtatgaatt ccaagctaaa cattcctaaa aaacttaaag atgagtctct tgaagaagaa
     6661 gttattcgtt taagagcag
//
LOCUS       DAZUMK010000131         6278 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000131 DAZUMK010000000
VERSION     DAZUMK010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.133"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(51..1181)
                     /locus_tag="ACG808_002479"
     CDS             complement(51..1181)
                     /locus_tag="ACG808_002479"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFS5048072.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1241..1468)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1830..3032)
                     /locus_tag="ACG808_002480"
     CDS             complement(1830..3032)
                     /locus_tag="ACG808_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5048073.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3092..3295)
                     /locus_tag="ACG808_002481"
     CDS             complement(3092..3295)
                     /locus_tag="ACG808_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFS5048074.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3437..4504)
                     /locus_tag="ACG808_002482"
     CDS             complement(3437..4504)
                     /locus_tag="ACG808_002482"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFS5048075.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            complement(4562..4669)
                     /locus_tag="ACG808_002483"
     CDS             complement(4562..4669)
                     /locus_tag="ACG808_002483"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5048076.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4832..5644
                     /locus_tag="ACG808_002484"
     CDS             4832..5644
                     /locus_tag="ACG808_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5048077.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6018..6209)
                     /locus_tag="ACG808_002485"
     CDS             complement(6018..6209)
                     /locus_tag="ACG808_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048078.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT         1858 a         1294 c          991 g         2135 t
ORIGIN      
        1 catgaaaagc cgactttttg ggacgctttt ttttgccaag cctcacattt ttatagccaa
       61 acttcttttg cgacttgctg caataattgg attttcttcc attgttcttc ttctgttaaa
      121 tggtttcctt cttctgttga agcaaaccca cattgaggac tgatacttag attttccaat
      181 ggtacaaatt gttgtgcttc ttttattcgt tcttttattt tctccgtatc ttccaaatct
      241 ggaaacttag aagtaactaa tcctaaaaca actttacttt ctttgttact ccaatgtctt
      301 aaaggagtaa aatctcctgc tcggtcagaa tcgtattcca aaaagaaacc atcataattt
      361 gttgaaaaca actcttctgc aatgggttca tatccaccgg aaaacagcca agaagaagcg
      421 tgattcccgc ggcagatatg tgttgtgatc gtcaaatctt ctggtttgtc ttccagtgcg
      481 ttattgataa tctctgtaca aatatgggcc atttctttcg gatcaattcc tttttgaaga
      541 agtgcttctc ttttagtttg atcagctaga aacgcccaat ttgtatcatc gaattgcagg
      601 taacggcaac ctttttcata aaaagcatga atcgctgctt tgtaagtaga ggacaagtcc
      661 tcataaaaat cagatagatc aggataaatc tccttgattt ttgaagaacc atcattagat
      721 aggatctctt gtcgtaaaat catcgtggga ctagggatcg ttgcttttgc aacaaaagta
      781 tcatctgttt ccactacttc tttcagaaaa gcaaaatctt ttaaaaatgg gtgatttgga
      841 ttgaaagata atttatctac tacccgaata ttataagctg gtgctgcttt tccgtggaat
      901 gtttgattat atccagtttc tggaacatag ccttcgatac catttagttc ttctaaaaaa
      961 tcaagatgcc aaaatccacg ccgaaattca ccatctgtaa ttcctttgat gccgactgct
     1021 ttttggtctt ccactaattt ttttatttcc tggtcttcga ttgcacgaag ttcttctttt
     1081 gaaagtaagc cttcttgaac tttttttctt gcttctttga tcgattgcgg gcgtaagaaa
     1141 cttccgacat gatctactgt aaatggtcta tttttgctca tttcactcac tcttttcttt
     1201 gttttttgta aaaaaaaatc cttttactag caaatactag taaaaggacg attatactcg
     1261 tggtaccacc ttttttcaga aaaaacattc tttctctcag cctgacaata aaatcagtgg
     1321 tcttggtaac gaaagactgt tcgtttttgc ctacctttcg acaaaaaatc ctctaaggcc
     1381 attttcgaca gtcaacttct agctttttac accaaccaaa gcctctcttg aaccattttc
     1441 tgtctacttt ccttctcatc ggatactatt agaataaaat tagaatagca tgtttgaaag
     1501 aaaagtcaat agggaataag aacaaacaaa aactaatttg ttattcttag taatttgttt
     1561 gtggaataca ataaagcttt tctaatatga taattgttca gtcgtccttt catcttagat
     1621 cacttcctta aaatgatcat acccgtttca accacataaa aaaggactta attttgaaaa
     1681 atttcccatc aaaactaagt cctttttatt atagttatgc aaatttgctt attttctcgc
     1741 tatttccgat acatcttgaa caggagcaat tcgactgaaa acactggtgc aaaagcattt
     1801 atttgtctta tggtagaacc gtggaaaact cagactggta ataaattgtc attttttgaa
     1861 agaatagcgc cctcttgaac attatcaatc gcacttacca cttgttgatc tgactttgca
     1921 tataagtgac cgtaagttcc taaagtgatt tgaatattcg catggcccaa tcgttcttga
     1981 atagatagcg gttgaatatt ctgctcaatt agaaatgcaa catgagaatg tcgtagcgcg
     2041 tgaagcttaa tcggtttgac acccgctttt ttagcagctt cttttaacca gttcgcaaaa
     2101 gaataaggtg gcgtaaatgt tccgtccaac tgagcaacat attcaaaatt tccaatacgt
     2161 ttttgcaagt cataccactg tctgagtttt aaggaaagct tcttccccaa cccaatcgtt
     2221 cgataagcac atttagtttt gggagtcgaa aagtaccaat catcttttgt attataaatc
     2281 aatgtcttct caatcgttat cgtgtttttc tcaaaatcta cattcgacca agacaaagct
     2341 tcaagttctc caatccgaat gccagtataa aagagcattt catacacaat cttccgatgg
     2401 tgggtttgaa tcttatcatc cttcaattca ttcatcactt tttgaaactc actgtgttcc
     2461 caaaactcaa ctttcttatt ctcagaagga agcgaagaaa catcttcaac gggattttta
     2521 gcaatcaccc cctctttaac agcccgatcc aatacaattt ttaggtgccc tcgaatccga
     2581 ttgatataag attttgataa cgtttcgcca ttaacttttt tccgtgaatt acgtgactct
     2641 gtctggctta gttcaaataa ccaatcctca atatcgtgac ttgtaatatc tcgaacacga
     2701 aaatgataga aaaactgcat acgcttttcg aaaatgaatt gcgcattctc atatgtttga
     2761 cttttgacct gtctctcgta ccattttaca aacctatcct tgtaaaactc ttcgaaagtc
     2821 atttgattgg tagaacacag ttgattagga attgtcttgt taaaatcatg tgtttccatg
     2881 tacttgtcat aagcaatttt ggcatctttt tgtgaagcat aacctcgttt agtcttctga
     2941 acctttcttc cattttcatc tatacctaga tacgctcgga agaaccattt ttttgtcttt
     3001 ttatcttggt aaatattttt aatttttggc atcgttccat tcctccctgt acttgagaaa
     3061 agctacttta atagtaccgc gcttgtactg tttattccgc aacttgaatg tgaaaaagat
     3121 gttcaattac tcgggatggc accacaccaa cttggcgatt attataaaaa tcaatacctt
     3181 caatccttgc aagatatgcc ttgctctctt taatcattcg tgcagcttga ctaggtttaa
     3241 agcccatctc tatcaaaatt gcacgattta ccatgaaatc acattttgtt tccattctct
     3301 cacctcctga ttctttactc cttttaaaat aatcctcttt ataatctatt gtttctcatt
     3361 cgatggttgt gttatactcg tcttgtcgag gcggagtacg ttgcacaact tgttgtgtaa
     3421 gcagtacttt gcttttttag tagcttgttt tctcatttga tatactttgt aattttcgtt
     3481 cataatactc aacctcctcc tgataatact ccgggtctgc cttcatattt tggagaagtt
     3541 tgatctgtct tggattcatt tctccttgtt caataatcaa aggcaacaag gaagaatgaa
     3601 acgtttgatc gacctgtgca ataaaaacta atgatggtga aacaccatga attaaccaac
     3661 tcatagtttt ctcaaaactt tgaggaattg tatctaaaga aatggtgatt gcgccataat
     3721 tgctaataaa ggctgcccat ttggcatcaa cttttgcacc tagatcattt ggatcacggt
     3781 cataaaaaca aattgctcca ttaagtaact caaagaacag tttggttaaa tcatgggttt
     3841 ttgaaataat ctttgccaag ctttgcgctt tctcttttcg ataccgtagt tcaaagcgat
     3901 tctttacttt tgcctcttct aaaggaattc ctcgtcgctt ccgttgttca tggtccttct
     3961 gataaaagca aaaacgacac tgactcttct ttgagcctaa atacaaggtc tcaccatttg
     4021 attcattgtt tatgatatcc gtaccgccat gagattcact cacttgaaaa gtggtccaca
     4081 catggcctag tattacctta tgcttaagtt ctggaatgct gagactcccc acaaaatcat
     4141 ctaaagtaaa atcaatccgt gtaaagttcc cctgataatc taatacctta cgaaaaaact
     4201 gttgccaatt tgtttttcgt gtttttagcc atcttgctaa gtgtttacag ccttgacctg
     4261 aaaactccat cacagttcct ttaaccgcat cgtcaatgga ataccgaatc gtgattactt
     4321 gattccacac gtattggcca atatagccct ttggcgcata atccagttcc tcaaaacgtt
     4381 caatgggcat ttcaataatc tcttcaatta gctttaaatg ttgattcgag aaaaagtgaa
     4441 tggttagata atcaatcaaa acacggttaa ctggaacgac ctcttcttgc aaactatcca
     4501 acacaagatt caatttttct tcacttaatt gtctcgttcc tttttctact ttcgataata
     4561 actatctcga aatccccact ttttctgcta aatatcgttg tgtccacccc ctttcttctc
     4621 gagaaactcg aatggcttca ccgagatcat gttgattaac ttggttcatc gacaaacctc
     4681 cttctgctat ttacttgtca aagaacctat tgaacttatt ttaagtacat ttttattgta
     4741 cgcctgtatt tattttaagt caatatgttt tgactttttt taagtacaca cgtatactaa
     4801 atttaagaac ttatcctgag gaggagtttt catggagata gacaagcaag cagtcggcaa
     4861 tcgaattcgg cagattcgcc aagaattaaa gttgagtatg gagaaatttg gcaaactaat
     4921 tggtgattta ccgcgaagta cagtcaataa ctgggaacgt gggatcaacc ttccaaaaac
     4981 cgaaaccctt caccaaatcg cagagatagg tcatgtcacc aatgaatatc ttttgtatgg
     5041 tgatcaagag aatcaataca ttttagaaat gctccaaaaa aaggcaggta agcttgatcc
     5101 taagattgaa ggtctcatag tagacgaaat caaacacgct gaccttgtta gtgaaaaaga
     5161 aatggatcga atgattgaat tttttattac aaatctcatt ccaccaactg aacaagacca
     5221 gtttactttc caatgtatcg atgaaaaaaa gcagctttat cttggttcaa ctaattttgg
     5281 taaggaagcc cagctctatt tgcaacatga taatcaaaat catattctgc acatgatgcc
     5341 ctttaccttc tccaacttta gtgtggatcg catactcgtc tatttagcaa atcaaaaatc
     5401 ctattcctat tttgggaaac atttacccaa ggaactaggt gagaaagcca tccttctata
     5461 ctcaattaat caattgacca gcgatgtgcg aattgccccg ttggtctatt cagaagaaac
     5521 cgcttcttat cagtacacag aggacaatca atatctacta gaacagcaat atctttatct
     5581 accattcgtg atggaagtcg agaaagaacg cctgttaaat gcagcgtatc catcatcaaa
     5641 atgaggtgac aaaattgata agcacgtgac aaagaaaata ctgctaggac actccccaat
     5701 gggtagttgt cacagtttcc tgttctttgt tagccccctg ttagaaccgg gggctttatt
     5761 cctgcagtcc ggctacgcct tcctgcaata gacaaaactc cagatcgggt aattcgcttg
     5821 tggcgaaacc cgacctggag tttgtctctg tattttgaac cctaaaaatt aatcaaacag
     5881 actgatattc tcataatcag cgcgaaataa gccattaagt acagttttcc tacctatttt
     5941 tgataacccg ctggttagtt aatattcctt ttccgagaga gataaatgat tggttaaatc
     6001 tatatacatc tcaaccttta aaaatgtttt cgaaatactt catcagtaca tgtcatacaa
     6061 attaccagtg cattctcaaa ttctagttct ttaattgatt tcagttgagg agcagttact
     6121 tttggcggaa atttccattt ttgtccaaac ctcttccccc gatgaaattt ctttgttgca
     6181 attattgcaa tgaaaaattt ttgtttcaaa aatatacata attacttcct ccaataaaaa
     6241 ctatctacct ctcaaattat ttttctctaa taagaaaa
//
LOCUS       DAZUMK010000132         6231 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000132 DAZUMK010000000
VERSION     DAZUMK010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.135"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            302..551
                     /locus_tag="ACG808_002486"
                     /pseudo
     CDS             302..551
                     /locus_tag="ACG808_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(795..1625)
                     /locus_tag="ACG808_002487"
     CDS             complement(795..1625)
                     /locus_tag="ACG808_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFS5048079.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            1833..3167
                     /locus_tag="ACG808_002488"
     CDS             1833..3167
                     /locus_tag="ACG808_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5048080.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            3208..4119
                     /locus_tag="ACG808_002489"
     CDS             3208..4119
                     /locus_tag="ACG808_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5048081.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            4116..4970
                     /locus_tag="ACG808_002490"
     CDS             4116..4970
                     /locus_tag="ACG808_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5048082.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            4967..5584
                     /locus_tag="ACG808_002491"
     CDS             4967..5584
                     /locus_tag="ACG808_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFS5048083.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            5586..6104
                     /locus_tag="ACG808_002492"
     CDS             5586..6104
                     /locus_tag="ACG808_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFS5048084.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
BASE COUNT         1856 a         1107 c         1245 g         2023 t
ORIGIN      
        1 atttctgttg aatatctaat aaataaagat ttcataggtt cttgttttta tacggcttta
       61 tttcttggac ttcttgaagc attttccatt tataaaaaat caaaaaaata aaatagattg
      121 ccgtaagaga tctttttcat ttcatttttt tctgaaaacc cgttgtttct gtcagtgaag
      181 cagcgggttt ttagatttat tgtctttaga cctaattgag cgcgccgagc gattgaaata
      241 caaaaccact tgctatgcgg gtggagcaat aaagggaaat agtttagccc gtacaaagta
      301 gatgtgtaaa taccacattg tattcacacc aaagtataga agaaaaataa tttataatca
      361 atatcgagcc aatattcagg agatcatcaa gagattgtgc aaatctaaaa tataaatttg
      421 ggtatcgaca cttttgggtg gaaggatact atgtgacaaa agtaggacaa aatgaagcga
      481 ccatcaaaaa gtacattcaa gaacaagaaa aacatgatag tgcgttagat aagctgagtg
      541 tacgagagta agacgatcct tttaagggct aaggagtagt acataaacct tttaagaggt
      601 gggcaaaact ggcaaaggca atatggcttg aacataaaat atgaaagcca gcgtctttag
      661 gcgctggccg gtgatttgag cttatagctc tagagaaaac cacccttttg aagggtggtt
      721 atgattaatg actttagtca gttttgctca caaagcgtgt ggaacaaaaa acgggtgttt
      781 ctacgggtag atatttattt cttgaatgga ggtgtcccat acatttttgg tgttactggt
      841 tctaattttg gtgcagcgcc ttcgatctta ggttctttca accattgata ttcgccttcg
      901 ccatccagtg ctttgccact agcccaagaa ccttcactac tttggtctcc ctctgagaag
      961 ttatagaagt cataagcaat gtcttttcgt tctttgtcac gcgggaaagt acttgggact
     1021 aagattgaac cttctttttc ttctaattct ttcactgcac attcccatag attttgatga
     1081 tagctatctc ttgcaaggag aacagacagc atatctttta cgccacgatc atcgatcatt
     1141 tcgtaaatac gtgctacttg aagtcttcct tgtgattcgg catttaaatt agctctaaag
     1201 tctgctaaca agttgccact cgcaatgata taggcaccgc tccatggatt gcccacgctg
     1261 tcagcaggac gaggtcctaa gccagctacg atcgcgtgct gtggattcat tccagagata
     1321 atggcaccga ttgttggatc tttttgcatc gctgcttctt gatcttcaac aggcgcaccc
     1381 tctaataaac gagcaaccat tgtcgccaac atttccacgt ggccgatttc ttctgttgca
     1441 atgtccatca gcatatcttt atatttttct tctcctcggc agctccagcc ttggaacagg
     1501 tactgcatag ctacactcat ctcgccatat tggccgccga tcagctcctg cagatatttc
     1561 gcgattaatg gattcggacg atctggtttt gcttcaaact gcaattcttt ctggtgtcta
     1621 aacatcaaaa ttcctcctaa tctattttta atacattctc attctgctac gaaagattaa
     1681 aaaattcaaa gaaagcgctc gcgaattata taaagtattg gtatcattaa taattttttt
     1741 agaatcatac cttataaatt gttattttca gaacaaaatg atatcgttat attaaaaccg
     1801 gcatcggtta cattaaaatt tggaggtaaa atatgaaaaa attattgttt acagggttgc
     1861 tggcaagttc agcattgttt ttattaggag gatgcggatc aagtaatgca agcgatgcca
     1921 gttcaagtgc agcaaattct tctgaaaaaa caagtgatgg aagaaaaaat attgatttct
     1981 ggtccttttg gggatccggt gcacgaaaag aagctatcga agaaatcatt gaggatttca
     2041 atcaatcaca aaacgagatc catgtaaatt ataagtatca gccatggggt gacatctgga
     2101 ctaaatcttt atctgcaata acagctggaa atgcacccga tgttattgta caggacataa
     2161 attccgttgc ccaaagagca gaagcacagc aagcaactaa tctctccaaa tatgtagaaa
     2221 aagacacttc taaggatttt tatccgcagc tttgggatac agtagaatat gaaggtgatc
     2281 cttatgcgat cccattcaat acagatacac aagtcatttt ttataataaa aagttattta
     2341 aagaagctgg aatcagtgaa caagatctac cgacaacttg ggatgaatta gaagaaacag
     2401 cacgtaaact agataaaaaa gaaggaaaag aatttacacg catcggtttt tatccattat
     2461 ggaatttagg ggctgatgta tgggcgctaa acgcagataa cgggactagt tggtttgaca
     2521 aagatgaaaa agtcaagatt gatactaaaa ataaagtgga agccttggag tggatccaag
     2581 actggcaaga ttattatggg aaaaatacga tcaataaatt agaagccgaa tttgggtctg
     2641 gtgtatctga tccatttatt tccggattag tagccatgcg tgcccaaaat atcaattatt
     2701 actctagttt gatggaaaat gcaccggatg attttgattt tggcgtgatg cagctcccag
     2761 aaaaagaaga aggttcaggc cattggtcgt ggggtggcgg attcgtcttg gaagtaccat
     2821 acggagcaaa agatcctgag gcctcttatg aattcatcaa atatctttct tctccagaag
     2881 tacaagaaaa attcggcgaa aaaagttttg atatcatggc gagcaagaca gccaatgaga
     2941 atttagtgaa taatgacaac ctaacggata aaggaaaaat gatctatcaa atggcggacg
     3001 aaaactttaa aaatacggtg atcacaccag ttcctttaga tgctccggat tacacgacat
     3061 tagtgaatga acagattgac cagattcttt taggaacaaa atcaccaaaa gaaggattgg
     3121 cagatgcaca aaaagcagta gaaaacttag tcaagcaaaa tcaataatcc atttttgact
     3181 gtctaggtac agaggaggca agaaaatttg aagcgtttat ctatccgaaa gaaaaaagaa
     3241 gcaagatggg cctatttatt tatctcgcct tttattatcg ggttcttggt atttatgcta
     3301 gggcctatgc ttttttcagt tattggtagt ttcaccgatt ataatttgac atccaagatg
     3361 aatttcattg gtctagcaaa tttcaaaaga atgttcgttc acgatgattt attttggaaa
     3421 tcgttataca acaccgttta tttcgtcatc ttcaatgtac cgctgacgac gattttcagt
     3481 gtgtttttag ccacagtact caatcaaaaa atcaaaggaa tcagtgtatt ccgtacagct
     3541 ttctatttac cggcagtctt atcaggagta gcagtctaca tcttatggat gcagctgctt
     3601 tctccatctg caggattgat caacacattt ctgggctggt tccatatccc tggaccagct
     3661 tggctgtttg atccacaatg gacgaaacct gcgttgattt tgatgaaaat atggagttct
     3721 ggaggagcaa tgctacttta cttagctaca ttacaaaatg ttcctagatc tttatatgaa
     3781 tcagcagaaa tcgatggagc aggcgtttgg ggaaagttca aaagtattac tttgccatta
     3841 atcactccta ttatttttta cgatgtgatc acctctacga tcggttcttt ccaaattttt
     3901 caagaagctt atgtcatgac gaaaaatgga acaggcggac ctgcaaattc tttgcttttt
     3961 tataatctgc atatgtggaa caaggcgttc gtagcttttg atatgggtta tgcgatggct
     4021 atgtcaatga tcctgtttct gatcgtgttg gttctgacgt ttgtcaattt gaagcttgca
     4081 cctaagtggg tctattattc tggaggtgac ggcaaatgat ggattatttc aaaaagacca
     4141 gtgtgaatcg gcaaacaaaa aaaatactaa gttatgtcct catgacagta ataggaatca
     4201 ttttgatcat tccattgctt tggatggtgt ttacttcatt gaaaccaatg gaagaaatcg
     4261 ttcgttatcc tccgaccttt ttcccagaaa aaatcgtatg ggaaaactat ctggatacga
     4321 ttgccgcctt tcctttttgg cgctatgcaa gaaatacatt attcatcact gtgctagtag
     4381 tgatcggtaa tgtcctttca aattctttta ttgcttatgg atttgcgaag cttgattttc
     4441 ctgggaaaaa gctgatgttt gctttggttc tttctacgat gatgatcccg gggtttgtaa
     4501 cgatgatccc tcaatatgta ttattctcca agatcggctg ggtaggtact tatttgcccc
     4561 tgatcgttcc atcgttcttc ggcaatgcgt tcaatatatt tctgatgcgt caattttact
     4621 tatcgatcaa taatgaattg atcgaagctg ctgaaatcga cggagcgaat catttgtata
     4681 tttggagtca tttgatgctt ccattgacga aacctgcctt gattaccatt gcgatcaatt
     4741 ccttcaacgc agcttggaat gatttcttag gtcctctgtt atatatccaa gatcaagaaa
     4801 aatacacgtt acagattggc ttgcaagttt ttcagaacca agcaacgaca caatggaatt
     4861 atctgatggc aggagcaaca cttgtcttga tcccgacgat tttgctgttc ttttttgcac
     4921 aacgttattt cattgaaggg atggatttga caggaggttc aaaaggatga tgaatcgatc
     4981 gattggtaaa aaagccaatg attatttctt agaaatcatt gaatggattc cacgtcttat
     5041 ccatttacag ctactatggt tcctgtgcgt cttgcctgtt ttcacattag gaactgcaag
     5101 tcgaacagtt ctgtatatga tttatcatta caaaaaaaat gaagagaaaa agctcggttc
     5161 actattctgg aaaaagtttc gtgagaactt ctctctttat ggaaagcaag atagtttagc
     5221 aagtgtttat tttcttcttc tattgatcga ttatcagatt ttccgctatt ggggtggggg
     5281 aattggctat acattgatgt atactacgtt gtttttgctt ctattaagcg cgcttctctt
     5341 tagttacaaa atcttgcttc aactggaaca aaaagaagct tcttggatta ctgcattttt
     5401 cttgtttttt aatgatttca aaagcgcgct tttctatttg gcaggcacgt tgatccttct
     5461 tgtcgcattc tggtttgcag gaccaatcta ttttgcttta ttaggtttca gtttcttgtt
     5521 ttattttcaa gtcctgcttt ttatccatcg tacgcaagaa aaggttctta aaaaggagga
     5581 ataacatgtt gcgtttgatt gctaaaatcg atgatagtat tatcaacaag ttcgccaagc
     5641 aacggaaaac ggatagtcga aaaatcaatt ttttgaaatt tatcagtaat atctctaatg
     5701 gtggttttat ttggttgctc cttgctgctg ttttagtctt gatacctgat actagagaat
     5761 atggtctagc cattcttatc ggcagtttga tctatttttt tgttggtttg ctttttatca
     5821 aaaaaacgac aagacggaaa agaccttatg aaaaaaatcg tcggcttgtc ctttacacaa
     5881 gtaaaccgaa gacttcttct tttccttcag gacatacagc gctagccttt ttatcagtag
     5941 ctgcgttgtt cgaattaagt ccagttgcag gaatattagc ctttttattt gcttgtttga
     6001 tcgcttattc gaggattttt ttacttttac attatcctac tgacatttta ggaggagcta
     6061 ttttaggaag tgttattggc atcctatgcg ctgccgttat ctgaattaat tgtacgaata
     6121 aagcatgagt gcgtaaatgc gcatttgtgc tttatttttt tgtttcgagg aaagcgtagt
     6181 tgtttattca tttgtcttaa taacttaagt tttgttaaat gagaacgtta t
//
LOCUS       DAZUMK010000133         6203 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000133 DAZUMK010000000
VERSION     DAZUMK010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.136"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..310
                     /locus_tag="ACG808_002493"
     CDS             <1..310
                     /locus_tag="ACG808_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048085.1"
                     /translation="VNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIP
                     PKSAISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN
                     "
     gene            complement(353..532)
                     /locus_tag="ACG808_002494"
     CDS             complement(353..532)
                     /locus_tag="ACG808_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048086.1"
                     /translation="MGKFNRALVFSAPLIIYALELWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(519..857)
                     /locus_tag="ACG808_002495"
     CDS             complement(519..857)
                     /locus_tag="ACG808_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFS5048087.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(873..1076)
                     /locus_tag="ACG808_002496"
     CDS             complement(873..1076)
                     /locus_tag="ACG808_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5048088.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(1091..1867)
                     /locus_tag="ACG808_002497"
     CDS             complement(1091..1867)
                     /locus_tag="ACG808_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFS5048089.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            2166..2486
                     /locus_tag="ACG808_002498"
     CDS             2166..2486
                     /locus_tag="ACG808_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5048090.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            2504..2935
                     /locus_tag="ACG808_002499"
     CDS             2504..2935
                     /locus_tag="ACG808_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFS5048091.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            3065..3526
                     /locus_tag="ACG808_002500"
     CDS             3065..3526
                     /locus_tag="ACG808_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HFS5048092.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKNELLGL"
     gene            3560..4195
                     /locus_tag="ACG808_002501"
     CDS             3560..4195
                     /locus_tag="ACG808_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFS5048093.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            4308..4943
                     /locus_tag="ACG808_002502"
     CDS             4308..4943
                     /locus_tag="ACG808_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048094.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(4936..5070)
                     /locus_tag="ACG808_002503"
     CDS             complement(4936..5070)
                     /locus_tag="ACG808_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048095.1"
                     /translation="MIKVIFKRITELPEFRLFVDYWYVWFFIVALLIGLDVILERKLK
                     "
     gene            5242..>6203
                     /locus_tag="ACG808_002504"
     CDS             5242..>6203
                     /locus_tag="ACG808_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5048096.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHT"
BASE COUNT         2227 a          989 c          996 g         1991 t
ORIGIN      
        1 agttaattta gaactttcat ccgctaactt aaaccgcaga tggtcagcta ctccatttaa
       61 gaaattaatt gctaaaggag gatcagtcga tgataataga atatactctg aagctgtacc
      121 gctaaatatt cctcctaaaa gtgctatatc ttgttacctt gcatttgaag tagggaaaat
      181 taattttaat aaactcctta atcaccaaac aaaaatgata tttactctaa atagaaccca
      241 aatccacaaa attgttgata tcaaaaatac aaacttccca atagagaaat tagtgaaaga
      301 attaaactga gtaatagtaa tatacattct agtttctctt ttctattcat ttttagtcag
      361 ccccctcggt tggctttttc gctctgtact cagcttcagc ttcatcaagc cccataaaaa
      421 tccaaaccat gtaaacaatc gttcctatca acgcttgcct gcttccccaa agttctaaag
      481 cgtagatgat tagcggtgcg ctgaacacta gcgctctatt aaattttccc atccgcttgc
      541 ctccttaaga tttccgaaaa atttgtttct aaaaattcaa gtgttttact tcttaaaaat
      601 agatatgtgt ctcttccttc aactggataa tagacaaatc catttttgtt tttctcgata
      661 tcgataatat ttctatatct tgggttcttt aaaactctag aagtaaacca atcatatttt
      721 ctgttaatcc gttctagcac ttctggcaac gtcatccatc taccagtatc atcagctttt
      781 tttaactcct cataatccac ttgggagata attacatagc cttctggaat tgggattttt
      841 gcttctagat attgcattag ctgttcctcc ctttacaatt cgtacatagt gataatcgaa
      901 tctataattc tgtttgcttc tgcagaagtc tttttaccgt ttaaaattaa agataagtag
      961 cttttgctaa ttccaaatct ttcagcaagc atggtgtaag ttaagaactt tgaactttcg
     1021 acatattctt tgattttttc tctatctcgt tgagtgattt ctgcaatgtc agtcatacta
     1081 aaactccttt ctaaccaatt tcctctaaat ccatttgagg gtaatatcct tcttttttta
     1141 gtaattcgta gataaataga cgccctttct gtgtccattt ggtattcatt acaattttag
     1201 tgccaccatc ggctttcggg atctcagttg tatgagattt tgtgtatcct tggttcatgt
     1261 gttttttgca caataaccat tggttaccga cttttttctg aatacctagt ttatgaagta
     1321 atttattcat ctgttgtgga gacatcccat aatctgctgc aatctgacta attgttactg
     1381 aatctgtaga agataatatg ctatctaaat aggagatttt aggttcgtac tctgcaatct
     1441 tttgttctgc gattaatctt ccagttcttt cttcttttag ttgagttgct aattgaatga
     1501 ttgtatctgg attaagcaaa gcttcttcta ctttttctgg agttagataa cctccatgtt
     1561 ttctaattgc tggcaacact tcacttgtta cccaacgttt aaattttttg gcagagggaa
     1621 gttttgattt taagattaaa ctgtacaaac cagattcgtt gatgattgtc atctctcttg
     1681 attgacctga ggtcgtgatt cgcgacccca tcttatcttc caaatctaca tgccgcgaca
     1741 aagcatcttt agtgtttgaa taacccaaaa cactcgcaac gtctttgcct acaaaatatg
     1801 gttcatcatt tactaaaatt gtccgaactt cgttttgttc gaaattaaaa atttgcggtg
     1861 tgttcatatt tctcattcct ttcttttata ttcgtaaaca aatttaacaa cttttttcta
     1921 aattcaattg acagtatctg agtaatactc tatactaaga gcataattaa ataagacata
     1981 aaaacattga ttttaaagct ttcttggcgg tctgcattta catatcaata gtgttttttg
     2041 ttgtcttttt agttgttaaa tctgtttaca agatatagta tagctcatta ctcagaatta
     2101 gtcaaattat ttctgcgtat ttttctaaac tttttttgta aacatttaga aaggttgttt
     2161 tatcgatgaa tacctatgaa ataataaaag acttggcgaa gaggaaaaag atatctatac
     2221 ggcagttaga aattaatttt ggatattcta acggttatct agcaaagtgg aaaaataata
     2281 ctcctaattc agatgaatta agtcggattg ctgactattt cgatgtctct gtagactacc
     2341 ttctaggtag agaagaaaga gaaaccccta aacatgtgga tttatcagaa gacgatactg
     2401 ttttttcttt tgatggaaaa gaaatatcta aggagacaat gcgtaaagcg attgcaattg
     2461 ctaaagcttt agaggaaaat gaatagttgg agtgatggtt tgtatgtatt tgaaattgaa
     2521 agaaatgttg agcgaataca atttgaaagt agtttatatg gagatgaaag agcctggttt
     2581 ctactatcca aaacccagaa tcattttttt gaatgaaaat ctatacggtg aaactgcaga
     2641 agcctttcat ttatctcatg aacttgcaca tttcagcgcc tctcattttg aattttcagt
     2701 tttgtacgat acttctacaa cttttcactc aaaatttgaa acagaagcag ataaaatagc
     2761 tattctaatt ttattaaata tctatattga gaacgaactg actgatgaat ctcagtttaa
     2821 tctcgaaaaa tttatggagt attattctat tcagaataag ctcagataca cttgttatgc
     2881 tgtctgccaa tgctatttta agaaaaaata ttcttacgca aggcaatatg tatgaataca
     2941 tcaagaatta ttaaaaaaac cgatttcagc aaaacattaa aaaagcccgt gctgcaacac
     3001 gggctctttc ctcatatatg agcttctaca aaaaaaatca tatcacagaa atgaggaaga
     3061 aaaaatggaa aaagagattt taataaaatc tccaggaaaa accttgatta aagtaactga
     3121 tgattctatt tcaattatta gaaaaggatt tataaattta gttaatcaag gtattaaagg
     3181 agaaaaaact atccccttta aaaatatatc agccgtacaa ttaaaaaaac caggtatgag
     3241 taatggttat atacaattta ccttgctagg cggaaacgaa agtcgcggag gtatattagc
     3301 ggccacgaaa gatgaaaaca ccgttatgtt tacaaaaaaa tattggaatg aaatggaaaa
     3361 tttaaaaaaa tatatagaga agcaacaatc tatattagat aataattcca aagaaacaca
     3421 aatttcaagt gcagatgaaa ttaaaaagta taaagagttg ttagatgaag ggattattaa
     3481 tcaagaagaa ttcgatttta agaaaaatga attgcttggt ttataatatt caatatcaaa
     3541 agagaaagag gatctattaa tggacggtat atttggtttt actggtattg ttttatttat
     3601 tataggttta atcatgttaa ttgtaagatt tatcaaaaaa acaaataaga aaacacctat
     3661 gattctcctt attatcggga ttatttttac taccgtaggt ttctcgcttt cctcatcaaa
     3721 cgaggcaaat gacaataaca aacaggaaag ctcatcatct gatactcaaa ctactacatc
     3781 taacagcagc aataaaagcc taactcaatt tgaagaattt ttagagacaa ataatcatga
     3841 ctggggtgct ttcttagata gttattattc tattactcca gctactgagc aaaatacagc
     3901 ttttactaaa tatattagtg gtaaaacata tacttttgaa ggaactgtta tcgaatcaat
     3961 gactactaga atagccatta tagcagacaa agagtacgat gataaaagtt ggaatgatat
     4021 ttctgcaaca cctaaagttt catatgtaat ttttgcgaag gacttaaaca atgctgatac
     4081 ctttacaaag ggcgataaag taacttttac aggagaaatt agttcaagag gatcgaacat
     4141 agaaaaatcc tatgctcaat gggatatgat taatagtaaa gtaagtaaaa aataaatgaa
     4201 ccacgcccca ctaagagtta gattgtgcac aataataaaa cctatacaaa agagtgccat
     4261 caggagtcac aatgcttgta aaggaaatga caaaatagga ggtaactatg gataatagca
     4321 atgaaaaact aaccaaaaaa gaatatcttg aattaatagt tgaaggcggt attggagcaa
     4381 ttccttatat tggtggcgtt gtgcagtcac tttattttgg gtctaaaaac gagaagcgtt
     4441 tcaaacgcat cgagaaattc tacaaaaatt taaacgaatc attagaaaac attcgcgatc
     4501 aaattccaga agatgttttc aacttagaga acaaggacca actcattgga atattcgaag
     4561 ggataaatga cgaggtagaa aaatcaaagg cacaaaataa aatagatatg tataaaaatc
     4621 tttataagaa ttgtttactt cgaattaaca acgcttcatg ggataacgag gagtattatc
     4681 ttcttgtact gaatcaatta acatcgattg agattcaatt gttagcatgc ttaatgagtc
     4741 gtggaaacga aaatttcact ggaaatatca gcatgtctgg ctattcacaa gagctaatag
     4801 atggtagctt aaatagatta tctgattttg gtctgttaga aaaaacgatt agctccatag
     4861 tattaggtga tgtcggcaaa caaaatatgg gctacaaaat atctcattta ggaattcaat
     4921 ttattaactc aactcttact taagttttct ttctaatatg acatctagac ctatcagcaa
     4981 tgcaacaata aaaaaccaga cataccaata atcaacaaat aatctaaact ctggaagttc
     5041 agttattctt ttaaaaatca ctttaatcat aataaacacc tctgattgga tgatctaata
     5101 cacatccaat tataccatta aaacgacaaa tagcacatcc ttgcgccggc aagcaaaaag
     5161 gatgtgcaaa aaataataaa acccaaacaa tgggcttctt tatagttcct attgtatcag
     5221 agaaagagag ctgattcaat tatggcaaga ttagtcaaac gtggaaatag ttggcaatac
     5281 gaaatttcat acaaaaaaga tgacggaaaa tacacgaaga taagaaaatc aggatttaag
     5341 acaaaaggag aagcaaaaga tgccgccaac gaattagaat ataacttgaa caaaggcctt
     5401 aaaggggatc gcaaaaatct attattatca gattactttg aggattggat gcaactttat
     5461 aaagaaggaa cagtatctcc tatcacttat agaaaatacg aagatacgtt aatgaacata
     5521 aagaaatata tgccagcggt attgatttct gatttagata gagtgggata tcaacgcttt
     5581 ttaaataagt atgctaaaga ccatgtaaaa tctaccgtta ttaagtttaa taaccatatt
     5641 agagcgtcgt tgaaagatgc cgtagaagaa ggattaattc cgtttgatcc aactagaaaa
     5701 gcagtaatca aaggaaaaga ttcattgaag ccaaaagaag ataaatattt agattatgat
     5761 caatttaaat ctttaatgaa actcgtagaa gaaaaccttt ctgcgcagta ctcttctcct
     5821 atgctcgtgt tagttgctgg tgccactgga atgcgatttg ctgaacttct aggattaaca
     5881 tgggaagata tcgatttcga agatcaaatc atcactatta ataaaacatg gaattataaa
     5941 ttaaatgaat ggggaaaaac aaaaaacgaa acttcaaata ggaaaatttc cattgataaa
     6001 catacgattg atctcttaaa aaagtttaaa atcaatcaaa aagaattatt cgagaatttt
     6061 gaaatcaaaa accctcataa ttttgttttt ttcaacttaa aaaatggatt agtttcatca
     6121 aacgccgtca gcaaatattt gcgcaaaaaa ttaaaagaat tagggattga aaagcaattt
     6181 actttgcatg gactaaggca tac
//
LOCUS       DAZUMK010000134         6190 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000134 DAZUMK010000000
VERSION     DAZUMK010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.137"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..239
                     /locus_tag="ACG808_002505"
     CDS             3..239
                     /locus_tag="ACG808_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048097.1"
                     /translation="MAKMNSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSK
                     VYSILLEGTIGYILLDSLYKGTYQSLENLSQKII"
     gene            371..1399
                     /locus_tag="ACG808_002506"
     CDS             371..1399
                     /locus_tag="ACG808_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048098.1"
                     /translation="MYWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWIS
                     LLRISLQRMKQGHSITYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTE
                     QAYLYFMFGVMNTYLEEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLY
                     VNLAMIHLYSRVFGTKKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNT
                     YYEKNRRLIFQYCMLVRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYS
                     ASADQLPDIVISDYPIDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSI
                     LEKLDFVN"
     gene            complement(1425..1865)
                     /locus_tag="ACG808_002507"
     CDS             complement(1425..1865)
                     /locus_tag="ACG808_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS5048099.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            2025..2531
                     /locus_tag="ACG808_002508"
     CDS             2025..2531
                     /locus_tag="ACG808_002508"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFS5048100.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            2699..4612
                     /locus_tag="ACG808_002509"
     CDS             2699..4612
                     /locus_tag="ACG808_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS5048101.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            4617..5618
                     /locus_tag="ACG808_002510"
     CDS             4617..5618
                     /locus_tag="ACG808_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5048102.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(5820..6026)
                     /locus_tag="ACG808_002511"
     CDS             complement(5820..6026)
                     /locus_tag="ACG808_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFS5048103.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
BASE COUNT         2272 a          940 c         1233 g         1745 t
ORIGIN      
        1 ccctggctaa gatgaattcg gtgattcaac aagcagaatc gttttgcgaa aggtatccag
       61 aatataaatt atcttttctt tcagaaaaca agatgataaa agtcgagttt tcttctcagt
      121 ttttactgag taaagtttat tctatacttt tagagggaac gattggttat attttactgg
      181 attcacttta caaaggtact tatcaatcct tagaaaatct tagtcaaaaa attatataag
      241 tttgcgtacg attcaaagaa aattaagaga acttaatact attttgaaaa actatcattt
      301 atcttgcagc ttgaaaaaag gtaatccgat agaaggagaa gaataccgta tccgatactt
      361 ttttcacttg atgtattggc agtcattcga cgaaaatcat tctgatatat tatcaagtat
      421 aaaaaataaa cgtcaactat tagaaaaatt tgcgaaacat gcgccatata cgcgtagtat
      481 agatagaaag aaatggatca gcctactaag aatttcgttg caacgaatga aacaaggcca
      541 ctcgattacg tatgtcccag aagagatgaa caaatttgtg catccattga taacaaaaga
      601 agagtttacg cattacatac tagagccttt ttttgaagca aacttctggt cgatgaatca
      661 catcactcca acagagcaag cttaccttta ttttatgttt ggtgtaatga acacctattt
      721 ggaagaagat ttgttggata ctccagataa agtacaagaa agttggggct ggattgaatc
      781 gtctattgta gaaaattttg aaaaaaactt caagatttgt tttacaactt cagaaaaaaa
      841 ttatctatat gtcaatttgg caatgatcca tttgtattca agggtttttg ggacaaaaaa
      901 gaaggtggat gcttttggaa aatcagcaga agacagagat tttcagcata tatttccagt
      961 gatgtatcca atcatgcaac agttttttag tggggtagct cgcagaattc cagagcttaa
     1021 tacttattat gaaaagaaca gacggttgat ttttcaatac tgtatgttag tcagggatat
     1081 ttttataaaa cacgaacaac ctgtcatatt ctatattcaa tcaaagtatg gtaaaacaca
     1141 ggaattatgg gcaaagaaaa ggatattatc tatgactgaa cgcccaataa aatatagtgc
     1201 atccgctgat cagttacctg atattgtcat tagcgattat cctatcgaca aaaaggcata
     1261 tgatcaaacg aatacacaga tattttactg gaatggacaa cctacaaaaa atgattgggc
     1321 gcgtctgaaa gctgaaatca taaaaatcag gaatgagaaa aatagatcca tattggaaaa
     1381 attggatttc gttaattaag aaaagcagat tgtttatgcc aaaattaaga cattgcaaaa
     1441 agattatttt ctttaaaatc gacaagatga ccagaaagca agagttcgtt gagctcttgc
     1501 tttcttttag ttttagaaag gaacatcctg attttatctt cgctaaatcc agtcaaaagt
     1561 tttgcagatt ggtcgtagat aatcggtttt ttcaataaag aaggggatgt acgaatctca
     1621 atatatagtt cactcaaact gagttcatct attctgtgaa caagattttt ataactaata
     1681 gaatgagtag agagcatttc ggtcacgccg ttgtttgtca attttagtaa gtggataaat
     1741 tcatttttgg ttagtggttg acgaattaag ttctgttcga taaacggtat atcatggacg
     1801 ataagccaac gttttgcgcg tcttgaagac gagcagccag aacctgtata caatttaatc
     1861 atcatacatt cctccaatag ctaagtattt atgaaataat aacatgaaga aatagtcgaa
     1921 aagtgccatt tagaggtgta aaataagaaa atgttgtcac gtataatgga actagctaga
     1981 taagaaatac tattaaatca agaaaaaaga aagaaggcac aagtatgtca aacacagccg
     2041 acgcaatagt agtgatcgat ttacaaaaag gggtatgttt tgattcttcg ccgatacacg
     2101 aattagaaac agtgacagca ttagtgaata aaagaatcaa aacatacgaa acgattggga
     2161 aaccagttat tttcgttcaa cacgaagatg ctcaattagt gccacaaaca aaaccatggg
     2221 agctattgtc ggaattatct attcctgagc acagttattt tgtgagaaaa acccatgcga
     2281 attcatttta taagactaat ttgagtgttt tattaactga attggactgt cattcgatcg
     2341 aattatgtgg ggcacaagca gagtattgta tagatacgac tgtgaagttt gctcatggat
     2401 taggatacca gcttatgatg tgtagaggag caacgacaac ttatgataat ccttttatga
     2461 aagcaaagga aacaatcgct ttttacgaga aaatttggga taaacgattt ctgacatttt
     2521 tggagcaata agttgtttct tcgtttaaaa aaagcagaat ggatactggt ttatccattc
     2581 tgctttatta cgtaaaatga gaaaataatc ctgttctatt tttatgggat aaaccattta
     2641 ttttttgtat aaaaaatgtt tgcgtttaca tataattctc ctagaaacat aaaaaggagt
     2701 gaatagcttg agaacaaaac aagtattaaa gggagcgttg gtcatcttat ttgctgtaag
     2761 tacaagcgtc ccgcaatgga cagtatatgg agagagcatt tcttccgaat cggaatctcg
     2821 ggaggtagaa accacagaaa ctactgatgc tggaaatgaa acgaaatcga gtacttcaca
     2881 aacagaaaca aatactgaaa caacggttga gacatctgtt actagcacag atcaagaaac
     2941 gaccgctacc acagataacc aacaaacaga agaagcaaca gctacaaaaa tgtcaaacga
     3001 cgaatcctct tctgctaaag taagtgacaa agagaatggg acagaaaatg atgatatcgg
     3061 taaaggtgaa aatttcaatg gtaaagaata tttgaattac ttcggtaaac agatgacaga
     3121 aatacttaat ggaccaacag aagacatcaa gtcaaaatta atagagttat tagtcactga
     3181 gcttgctgtt aatgattatg gtgtagataa agagattatc aaacatctga ttgctactta
     3241 tctattgtac gatatcgctt ttgcaaaaaa cagtgtgttg atgtcgatgc ttggaggtac
     3301 agatgatggg acaacgccta cagaaaacac aggcttctat ccaaatacaa aacaacttca
     3361 tcaaactgta ctaaacaaac aagatggcaa aatgatggat gtatatgcat attatgtcga
     3421 tcaaggttca gataaaacag tcatgatcca tggcggattt cgagggaatt ggaataacgg
     3481 gatcgttaca gaagaataca atgattttta taaggccggg tataatttat tatttgttga
     3541 ttccagagcg actggtaata gcggaggcga ttatgtgacc tatggacaat atgagtcaga
     3601 tgacgtcctt tactggataa atcaagaagt tcgagagcgg ccatctcaaa agattttact
     3661 ttatggtggt tcgatgggag cagctacgat gatgtctgta ttagcgaaag atattcctgt
     3721 caatgtcaaa ggaattattg aaaattgcgg atttgcatca attgatgaac aactacgttt
     3781 tacgtacagc cagacagtag cccccgcact accggatgcc ataaaaaatc agttagacat
     3841 cattggggat caagagcatg aagatttatt tatgggcctt ttgaaacaat actattttga
     3901 tcaggagatg catttggata caaaagctgc tctacctacg attggaatgt cagattcatt
     3961 gcctaaattg attatccatg gaacagcaga tgatgtagta cctgtgtcta acgcgcaaaa
     4021 attatatgag ttatcaggag ggtataaaga tcttttatta gtagaaggag cagggcatgg
     4081 aaaggcacag gaagttgacc atgctgccta cacgaaacat gtaacggact ttttgaaagt
     4141 agtgttccat gatcagataa acgtaaagta tgtggatgaa aacaataaaa gtctactcaa
     4201 agaccaggac gaaatccagc tttacggagc ttatggagaa aattatatga cagagcaaaa
     4261 gacattcgaa ggatacgaat tagcaaatgt tgaaggaccg actgaaggga tttttaatga
     4321 gacgatacca actattattt tctattacaa aaaaatccca gtagctccac caaaaaagca
     4381 agatccgaca gagaatgcag acaataaggg aaagaatgaa cctcgagagg atcaatcaag
     4441 tgatgatacg caaccgattc agatgacagt gaaagtacaa aaaaagacag atgcaaaaaa
     4501 aagaatgttg ccgaaaactg gagaaaagaa acattcgctt ctcatttatt caggtggatt
     4561 tttatctgtt agcacgatta tcacatggat ttggcgaaga aagagagggt aaatgaatga
     4621 aatggttcaa gcgaatagtg attgcagtac cagttatcat cgtagttatt ttagtcactg
     4681 tatttttgat taatcaaatc acaccaaagc cgattagctt gcttgttaga aaacaatttg
     4741 acacaagtga aaaagaacaa gtatatgccc gaccagatga tttccagcaa aaaatcgaac
     4801 aaatcgatat aaaaaaagaa atatcctatc cttcaaagta caaaaataat aatatggata
     4861 tctacacacc gaaagtggaa aagaaaaaat taccgattct tttctggatg catggaggcg
     4921 catatgtagg aggggataaa aatgattgtc gtgattattt ggaatatcta tgttcagaca
     4981 cgcagcaaat catcgtaaat atagattatg aacgatcacc ggaagcaaaa catcctacac
     5041 cagttatcca attgaacgaa gccattcaag cagctaaaaa agagataaaa gatcaagcag
     5101 attggtcgaa tatttcgatt ggtggaaatt cggcaggtgc acaaattagt ggagaatatt
     5161 tgttagcact tcaaaatgaa gaaattaaaa aagctgatca tcttgatccg actttggaga
     5221 acaagcagat tacgaagttt atttctttgt ctggtctgtt ggatccttct gcacttactc
     5281 aagtaagtga taaaatctct agctttcttt atgaaaaatg tggatgggct tactttgata
     5341 ataaggactt tgaacaatca gagaaggtca aaagtttagc cttggtcaga catgcggatc
     5401 gttggacgca aaacacattc ttaacggatg gaaatacaaa tacgtttacg aaacaaatgg
     5461 aagaagtaac ttctgtattt gagaaagcgg gggtgaaagt cacaaaagta gattatgcgg
     5521 agaaaaatgc tgttttgaac catgaatatc agtttgattt cagcaataaa caagcgcaag
     5581 aaacatatca aaagttagta atgtttttca aggagtaaag tcttatgatg caaaagaggt
     5641 tgtaatctgt cacttagctc caagtcacaa ggagcaattt caataaacaa gcttttcatg
     5701 gtttatgaat attgtaactt gtgactgagg agttagagat ggcaaggggc accttttatt
     5761 cgaaatttgg gaatatcaca cgattattgt cacgatctct tttacgtaaa ttttataagt
     5821 cagatttgtt gttttgatcg tttaaaaaaa gcaacaaatc cgaagcctgc ggaggcaaga
     5881 agggacaaaa agccgagtgg taagcaaaag aatgctggtt ccactaatat tccgttgtta
     5941 ttgatataag gctgggaaca aagatgaata gcaaataaaa tcaatgcact agcgaaaaaa
     6001 agcaaagaaa tatggatagc tttcataagc agcacctcct tttttataat tgtatgataa
     6061 atagattaag aatctataaa tgtgacaagt ttgttaggat gtgaataaaa tgatcatcta
     6121 gcgaacttga aacaaagatg gctaatgata agcaattaat gattcagcaa tcagataggt
     6181 acaaagttta
//
LOCUS       DAZUMK010000135         6173 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000135 DAZUMK010000000
VERSION     DAZUMK010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.138"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            725..811
                     /locus_tag="ACG808_002512"
     CDS             725..811
                     /locus_tag="ACG808_002512"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFS5048104.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="ACG808_002513"
     CDS             complement(932..1621)
                     /locus_tag="ACG808_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048105.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="ACG808_002514"
     CDS             complement(1628..1906)
                     /locus_tag="ACG808_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048106.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="ACG808_002515"
     CDS             complement(1866..2780)
                     /locus_tag="ACG808_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFS5048107.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQVFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="ACG808_002516"
     CDS             complement(2762..3142)
                     /locus_tag="ACG808_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFS5048108.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="ACG808_002517"
     CDS             complement(3592..3879)
                     /locus_tag="ACG808_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFS5048109.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="ACG808_002518"
     CDS             complement(3899..4123)
                     /locus_tag="ACG808_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFS5048110.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4793)
                     /locus_tag="ACG808_002519"
     CDS             complement(4578..4793)
                     /locus_tag="ACG808_002519"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048111.1"
                     /translation="MEKMVAAMEMKKEILMTKKHLLFGSILLGILSFSPMLLSVANKG
                     NKKGELKLNPNYYNSQNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="ACG808_002520"
     CDS             complement(4763..5296)
                     /locus_tag="ACG808_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFS5048112.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..>6173)
                     /locus_tag="ACG808_002521"
     CDS             complement(5317..>6173)
                     /locus_tag="ACG808_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFS5048113.1"
                     /translation="IVSRMREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEK
                     ILSLRFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYS
                     LQQFNELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPI
                     RQELTAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALAL
                     ADKQKEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLL
                     SDLDNLFQ"
BASE COUNT         1634 a         1152 c         1041 g         2346 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaacaaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atacttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DAZUMK010000136         6172 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000136 DAZUMK010000000
VERSION     DAZUMK010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.139"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            107..2548
                     /locus_tag="ACG808_002522"
     CDS             107..2548
                     /locus_tag="ACG808_002522"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFS5048114.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2878..3582)
                     /locus_tag="ACG808_002523"
     CDS             complement(2878..3582)
                     /locus_tag="ACG808_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048115.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3769..4014)
                     /locus_tag="ACG808_002524"
     CDS             complement(3769..4014)
                     /locus_tag="ACG808_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048116.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(4031..4882)
                     /locus_tag="ACG808_002525"
     CDS             complement(4031..4882)
                     /locus_tag="ACG808_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFS5048117.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            5048..6052
                     /locus_tag="ACG808_002526"
     CDS             5048..6052
                     /locus_tag="ACG808_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFS5048118.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         1985 a         1067 c         1305 g         1815 t
ORIGIN      
        1 gaatatttaa ggattgttaa aatttaatct tagaaaaaaa taactggaac aagagctatt
       61 tgtttttaca gcaaagaatg gtatattttt gtaggaggtg gcttacttga aagaagtcgt
      121 tatgatagat gctgcaagaa cgccgatagg aaagtatcgc ggaagtttaa gtccatttac
      181 tgcagttgaa ttaggcacac ttgtgactaa aggattatta gataaaacaa aactgaaaaa
      241 agataaaatc gatcaagtaa tatttggaaa tgtcctgcaa gctggtaatg gtcaaaatgt
      301 tgccagacag attgctttga acagcggtct ccctgtagat gtcccagcaa tgacgatcaa
      361 tgaagtttgc ggatctggta tgaaagcagt gatcttagca cgccagctga tccaattagg
      421 tgaagcagaa cttgtgatag ccggcggaac agagagtatg tctcaagcac ctatgctgaa
      481 acagtatcag tcagaaacaa atgaatatgg tgaaccaatt tccagtatgg tcaacgacgg
      541 attgactgac gcattttcaa atgcacatat gggattaacc gcagagaatg ttgcaacaca
      601 attttctgtg agcagagaag agcaggatcg ctatgccttg tcgtcccagt tgaaagcagc
      661 acatgctgtc gaagccggtg tattttctga ggagatcatc ccagtcaaga tttctgatga
      721 agacgtgtta tctgaggatg aagcagttcg tggaaatagt acattggaaa aactgggcac
      781 gttacgtaca gtattctcag aagaaggaac tgtaacagca ggaaatgctt ccccgttgaa
      841 tgacggtgcc tctgtggtga tccttgcatc caaagaatac gcagaaaata ataatctgcc
      901 ttatttagca accatcaaag aagtagcgga ggtcggtatt gacccaagta tcatgggaat
      961 cgctccaatc aaagccattc aaaaattgac agatagatca ggaatgaatt tatcaacaat
     1021 cgatcttttt gagatcaatg aagcatttgc agcttcttct atcgtagtta gccaagaact
     1081 gcagttagat gaagaaaagg tcaatatata tggaggagca atcgcattag gccatccaat
     1141 tggtgcaagc ggtgcacgta tcttgactac attagcttac ggattgcttc gtgaacagaa
     1201 gagatacggt attgcttcat tatgtattgg tggaggttta ggcttggcag tcttgttaga
     1261 agcgaatatg gagcaaacac ataaggatgt gcaaaaaaaa aagttttatc agcttacgcc
     1321 atctgaacgg cgcagtcaat taatagaaaa aaatgtattg acacaagaaa ctgcgttgat
     1381 ttttcaggaa caaacacttt ctgaagagct ctccgatcac atgatcgaaa accaagtgag
     1441 tgaagtggaa atcccaatgg ggatcgcgca gaattttcaa atcaatggca agaaaaaatg
     1501 gattccgatg gcaacagagg aaccatctgt cattgcagct gcaagtaatg gtgcaaaaat
     1561 ctgtggaaat atctgcgcag aaacacctca gcggctgatg cgagggcaaa tcgttctatc
     1621 aggaaaatcg gagtatcaag cagtgatcaa tgcagttaat catcgaaaag aagagctgat
     1681 tctttgtgca aatgaaagtt atccttcaat cgtgaaacgc gggggcggtg ttcaagatat
     1741 ctctactcgg gaattcatgg ggagtttcca tgcatactta tctattgatt ttttagtgga
     1801 tgtaaaagat gcaatgggcg ctaacatgat caactcgatt cttgagagtg tggcaaacaa
     1861 gttacgggag tggttccctg aagaagagat tctattcagt attttaagta attttgctac
     1921 agaatcattg gcttctgcct gttgtgaaat cccattcgaa cgcttaggga gaaacaaaga
     1981 aatcggtgaa caaattgcca aaaaaatcca acaggcaggg gaatatgcga agcttgaccc
     2041 ttaccgtgca gcaacgcaca acaaagggat catgaatggt atagaagctg tagtggcagc
     2101 tacaggaaat gatacacgtg cggttagcgc ttcgatccac gcctacgcgg caagaaatgg
     2161 attataccaa ggattaacag actggcagat caaaggggat aaactagttg gaaaactaac
     2221 ggtgcctttg gctgttgcca cagtaggcgg agcttccaat atcctgccaa aagcaaaagc
     2281 atctttagct atgttagata ttgattcagc caaggaacta gcacaagtaa ttgctgcagt
     2341 agggttagct caaaacctgg ctgctttgcg tgcattagtt acagaaggaa tccaaaaagg
     2401 ccacatggga cttcaagctc gttcattagc gatctcaatt ggggctattg gtgaggaaat
     2461 cgagcaagtt gcgaaaaaat tacgtgaagc agaaaagatg aaccagcaga cagctataca
     2521 aatattggaa aaaatccggg aaaaataaaa aagcaatatc tttagaagaa taaagcaaga
     2581 tttacagtta ttttatttac tgcagatctt gcttttttct ctatcagaaa atatgtttat
     2641 tattagaatt ttttatagtt aaggttaggc atcttaagaa atcaggcaac tccaagatat
     2701 cttcaggtag aagagaagtc gcctaacatt gtaggatcat aggtgatttg ttggacaggt
     2761 caagctgata tttgagcctg atgttagaag gaatttcttt ttacttgctg tagaaattat
     2821 tatatgacta aaaaggctgg gattttaaat cccagccttt tatgacaaat agaattttta
     2881 tcgatcagta atcgctgtcg ctgcaaattg ttcgatcaaa ggaatcaaag tcaatttgac
     2941 tttgtatttc cgttcgagtg ctttttcgat aacgaactca ttttcagtag tcatagaaat
     3001 agttgctaat ttacgaggtt catcagcatt tttttcatta ataactactt catgttcatt
     3061 ttgttctgtg taaaatttag gatactcagc aacggctttt gaaaaatcag caaaacttaa
     3121 agcggcattc gtatcagttg agcgtgtatt ttcagttgtg tccgcttttg gtttttccgt
     3181 tttttcttct ggcacactga ctgtttcttc tgtaactggt gtcgcaacag cttgcgcagt
     3241 tggttcatcg tttttcaatc gttgatcatt ccagtaagac acgatttcaa taagaagatt
     3301 gtcacggaat cgttcgaaaa tcccgttttc tcctgaacga attctcttta atacagtatt
     3361 gaagttgtca tcgattggca cgttgatccg tgtatcttca tttgttttcc cagctaatcc
     3421 gatcgttaag atttgacggt ttttgggatc aagcatgact tgtaacggca aaccataacg
     3481 tcgcaagctc tcttcttcta gagaacggaa taattgtcct cttacaccat ccaatgaagg
     3541 acggaattga tttaataatt tttgtttctg ttttttcttc atatgttctc tccttcgctc
     3601 ataccctttc gattataaga aaaaaacagc caaatgaaaa ggagtaatgc aaaaaaactt
     3661 gcgtatacgc aaattacaac agaaaaattt gcgtattttc ccgttttttg aaagaaaaat
     3721 aaaaaatgga acggattatg tatatataca taatccgttc cattctttct attttttcgg
     3781 tatcaaggca aaaacaggta gttcaggtga ataaatgact tcatgttcgt gccagggttt
     3841 gaccattagc gaatgatcga accgtttttt cagttcttcg atgatttgat catgtgtcca
     3901 gtttcttgca ggaaaatgtt ggattttctc tgcatcgatc aaaaagacaa aatcggaatg
     3961 aaaagtctgg gtaacttctt ccgagagaga attcaagcgt tcgattggat ccataaaatc
     4021 acctttcgac ttaaaattgg gtagtatctg ggtcttttgc tttgccgact acaccatcta
     4081 actgatcgat ttgtttcatt tcttcttctg ttaattcaaa atcaaataat tcagtatttt
     4141 ctttgattct gcttggtgtc actgacttag gaagaggaag gaattcgtgt tgcagcgacc
     4201 aacgcaaagc aacttgtgca atcgtcttgt catgtgcttc agcgatttgt ttcatttcag
     4261 gcacatcgaa gattttacca gtgcctaatg gactatatgc ttcgaggatc atatcatgtt
     4321 tttttgcata ctcaactaca gttgtctgta attcgcctgg tgcaaggaaa acttgattga
     4381 ccatcggcat aatttttgct gtttttgcta gtgtatctag atggtggggt aagaaattgc
     4441 tcacaccgat tgctttgatt ttacctgctt catacagttc ttcaaatgct cgccatgtat
     4501 ctgcatttgc ttgttcccaa ttatcacgaa aagcaacagg gtttggccag tggatcaaaa
     4561 acagatctaa ataatctgtt tggagcttct tcaatgattc ttcaaaagaa gacatcacaa
     4621 gttcatagct gtggtttgca ttccataatt ttgttgtcaa gaatatctct tctctaggaa
     4681 ttccactttc cttaatcgct tggccgacgc tttcttcatt cttgtatcct tgggcagtat
     4741 cgatatggcg ataacctgca gccagcgctt ctttaacaga ggaaacagcg acatccccat
     4801 caggtgtttg ccatgtgccg aatcctacta ccgggatttc gtaaccattt gatagacgat
     4861 aagtgcttgt taatgaattc atactttcag ctcctttttt attcgttcaa ctataagtgt
     4921 agcgcatttt tcgaaaaaaa gaatccattt tgtttctact cttgaaattt ttgtttaaaa
     4981 gaaaatgatt ttgattctgg tatttttgat tttttttccg taaactaaga cagaagggaa
     5041 tgagaaaatg gatatctatt taaaaaaagc aattctgcat attattgaca gagaaactgg
     5101 ttctccagtc ttttcacaaa aagagctgga tctgacaaaa gaatatattc gtgattttct
     5161 tcaaaagaag atccaaaaaa tctcttctgc tcaaacaaaa actggacaac tagcagaaga
     5221 aagcatgatc gttgaatcac ttaaaatcgc tcaagaaaat tttgtggaag caagtgaagc
     5281 aatcgttcag cggtggttcg atatctacca agaaagtgaa gaagcaccta gtgcagatgt
     5341 atttgttgtc ttgtatgaat tagatacagt gatgtactta tctttattga aagtcaatta
     5401 tcgtgaggct tacactcact ttgttgaagc agacgaagca gggatcgaca accgcttgat
     5461 tttgaatcga gcaattctag gggcgaaaac acaaaaagca gatgaagcat tcgctgtgaa
     5521 tttgactgat tggacctacg aattaattga aaaaaaatat gagttttctg gcaaaaaaga
     5581 gctttatttt tctggaagag tgatcgaaag tgttcctgca ccttctttag aagaaaatgt
     5641 aaaagtgatc caaaaagtag ccaagcatct tggaaaaaaa tttgagacag aagaatttga
     5701 tatcatggct gatttgaaag aagctgtcta tgatacgata gaagaaaaag gtcggttgga
     5761 tcatgagatg attgcggaaa aagtcttcaa ggaaaacatc acggcaaaaa tggcttttaa
     5821 agaagaagta caagagcaag gatttgtccc agaagcacct cctgtaaaag aagtacaaga
     5881 aatttctgag aaaaaatttg gaaaacaaaa attcaagcta tcaaacggga tcgaactgat
     5941 cgtaccagta gatgtatatc gcaacccaga tttgattgaa tttgtgaata atccggatgg
     6001 tacgatatcc gtgatgatca aaaatgtgga tgaagtattg aatcgtttat aaaaatgact
     6061 agaatattag gtgttcaagt accagtgatc agttaaatga attgtgtcaa gagaaggatc
     6121 ggaaaagacg aatataacaa aaaaagagcg tgagacaaaa gtaaagaaca ct
//
LOCUS       DAZUMK010000137         6115 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000137 DAZUMK010000000
VERSION     DAZUMK010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.140"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            172..1476
                     /locus_tag="ACG808_002527"
     CDS             172..1476
                     /locus_tag="ACG808_002527"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS5048119.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEDTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            1584..2441
                     /locus_tag="ACG808_002528"
     CDS             1584..2441
                     /locus_tag="ACG808_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFS5048120.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(2553..2873)
                     /locus_tag="ACG808_002529"
     CDS             complement(2553..2873)
                     /locus_tag="ACG808_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFS5048121.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            3043..4002
                     /locus_tag="ACG808_002530"
     CDS             3043..4002
                     /locus_tag="ACG808_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS5048122.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHSVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFRRSIQDRTTDYIQFNHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPAGRQAIDIENRLNQWFQSVPKHLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNK
                     VSETFIQSVASKRNHIPRKSLHYRTPLEVFLSYVGKDDLSSLI"
     gene            4092..4487
                     /gene="dhaM"
                     /locus_tag="ACG808_002531"
     CDS             4092..4487
                     /gene="dhaM"
                     /locus_tag="ACG808_002531"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFS5048123.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            4462..5472
                     /gene="dhaK"
                     /locus_tag="ACG808_002532"
     CDS             4462..5472
                     /gene="dhaK"
                     /locus_tag="ACG808_002532"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFS5048124.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            5500..6105
                     /gene="dhaL"
                     /locus_tag="ACG808_002533"
     CDS             5500..6105
                     /gene="dhaL"
                     /locus_tag="ACG808_002533"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFS5048125.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
BASE COUNT         2054 a         1103 c         1325 g         1633 t
ORIGIN      
        1 tgaagatgaa aaattgaaaa aaagacaaga attacttgaa gaagtgtata cggaacgttt
       61 tggaacaaaa gaagagagag aaacggtccg tttttattct gtctccgaag agaaaaatct
      121 agatacgaca ttcattgcag atttgtataa agaaaatggg gtggaactga aatgatctca
      181 atcacccata caggtaattt attcttagat acttgtttgt ctattatgta tttttttctc
      241 gtttcttatc cgattttagg cggatttgta tggtttattg gtgtctggtg ttatgtgttt
      301 ttatacaaac ataagcaaaa agaatgggta gatgttccct tgagtgtcga accgttcatc
      361 acgatcatgg tgccagccca taatgaagaa attgtgatcg aagatacaat cgagtatttg
      421 atgacgaaat tgaattatca caactatgag gtattagtga cagacgatgg ttcaacggac
      481 caaacaccag aaatattagc tagactgatg aaaaaatacg ctaatcttcg tgtggttcgg
      541 atcgaaaaaa ataaaggaaa agcccacgcg tttaatatcg gacttgcctt tgcaaaaggg
      601 aaattgattt taagcaatga tgcagacacg gtaccggagc cagatgcact gattaggtat
      661 gtgaattatt tcattcgccc tggtgcaaga catattgctg cggtcactgc aaatatggat
      721 gtccaaaatc gtaccaaatt gattgcaaaa tctcaaacgg ttgaattctc aagtatcgtg
      781 ggcatcatta aacgaacaca gtcagctgtt ttcggtggtc tttatgcgta cagtggagca
      841 aatacgatgt atcgaaaaga agccttgatc gatgtgggcg gattcagaca agatcgagcg
      901 acagaagaca tcagtattgc ttgggatcat cagttaaatg actggttatc tgtttttgct
      961 cctgagatta tctttttcat ggaagtacct gtgacgttga agatgctata tcgacacaga
     1021 aaacgttggg caaaaggtgg aacagaagtc tggctgacga atttcaaaaa agtgatgctc
     1081 catcctttta aacatattgg aagaacgatc atcttcattg atcaaacatt gagtattgtc
     1141 tggtcaatat tcttttgtat ttccgtagtt ctttttgctg gattaatcgg gcattatgta
     1201 tatcaaggaa actacgaaca gatttatatt acttttacat tcagttttgt gttcatctgc
     1261 tttgaaatgg tcgcaggatt tttccagttg ctagcttcgc tgattgtaga tgatcgacga
     1321 agaaaattga aataccttct tttcgcacca ctatatatgc ttttgttctg gatagtgaac
     1381 gcgataacga tcgtgacgac tttcattcca gcagtcaaaa cgattttagg ttatggaagc
     1441 gggacatgga aaagcccgga acgtacaaaa aaataaaaga tgcaccgttt tttagacaaa
     1501 cagcctgttg tgtttatggt tcttccaaac atttttgctt ataataagaa attagttaaa
     1561 aagtagtaag gatgggaaaa gtgatgaaaa aaagaatgaa gccgttaatc tggccaattg
     1621 ctgtttccag tattttctta ttgggacaga cagcttttgc ggaaacttct gaacctgctc
     1681 aaacagaacc ttcaacaacc ataagctctt ctggtgctga aaacggtgcc tcggaaacag
     1741 aaatgacttc caatgtaccg acagataatt caggagaaac gatttcatct tcagaagata
     1801 catcaataaa tagttctacc tcttctgacc aaatcgatca tttgcaagac ttggaaaata
     1861 aaagaaaaga actcgaagaa gcattagaag ctgcaaaaaa caaattgaaa gaagaccaag
     1921 cagcaatcga tcaagattta gctgatctta aaaataaagt gggaaatgac cgtgcacaac
     1981 ttgagaaaaa gcttgccgaa gcgaaagcaa aagcggaagc tgtccaaaca gatcttgacg
     2041 ggaagatcca acaagcaaaa gaattcaatc agcaattaaa tcaggttgct caaacagctt
     2101 ctggaaagct aagtcaaact caagcggata tcacagcggc cagtcaagca atccacggaa
     2161 agatcagcga agcacaaaca gttgctaatg attttgaagc aaaaaaagct gacttagcag
     2221 ctagtttcca acgaataaaa gatattttgc attcggcagg agaaaaatca gtgacctctg
     2281 gaagcacgac agaaacagtt cagcaaacga cagagccatc caaatcaaca agtcagactt
     2341 tgccaaaaac aaatgaaaaa acagcttctg tcagttattc cttagcaggg gcgggtgtgt
     2401 tagtcgcagc agcttacgga atcatccgca aaaagaaata aaaaagagag gatgggaagg
     2461 tagagtacct cgaagttaga ctgaaaaatc taattcttga gtgccactac atgtcccatc
     2521 tctctttagt tattaaaaca gatgtcccct acttatctcg tggagacagc tgttccacta
     2581 catgtgacca tcagcatgcc attgtcgctg cctaatactt catagtccat ttttacgcca
     2641 atgatcgcat cagctcctaa tgcttttgca cgttcggcca tttcggcaag tgcttcttct
     2701 cttgcaccca ttagttcgtt ttcatagctt tttgagcggc cgccaaagat atttcgcaac
     2761 cccgcctcca agtctttaaa tgcatggatc ccagtaatga cctcaccaaa aacgatccct
     2821 ttataagcta ctacggcttt tccctctaca ctgttcgttg ttgttgtaat cattcgatca
     2881 ctcctctctt ttaattaaag tataacaaac ttcaagaagc actacacata ttttcccaaa
     2941 attagaaatc gccgattgta aaattaagct agacaactaa aaaagtcatt tgtgctacac
     3001 tcaaaatagt tccgtcgaaa gaattataag gagtgagtac aaatgaccta tacccatctt
     3061 acaccaactg aactcgtaat gatagaagct tatttcaatc aatcacactc tgtttcaaaa
     3121 gtagcccatc tggtacaacg ttctcgacaa accatttata atgtctatcg ttttcttaaa
     3181 tctggtggaa gcgcaataag ctactatgaa caatataaga aaaacaaacg taactgcgga
     3241 agacgtccga ttgtgttacc agatgggcaa caagaataca ttcaaaaaaa ggtggctcaa
     3301 gggtggactc cagatgtcct tattggtcga gcagagtttc ctattgcttg ttcggtacgt
     3361 acactatatc ggatgtttaa agataaaacc tttgaccctc acgatttacc aatgaaaggg
     3421 aaaagaaaac caaacggaca taaagaaaaa agaggaaaac aagcttttcg ccgttcgatt
     3481 caggatagaa caactgatta tatacagttc aatcatgagt ttggtcacct agaaggtgac
     3541 accattgtcg gtggaaaaca taaaagtgcg gtgatcacgt tagttgagcg attatctaaa
     3601 gtgatcatca ccttgaaacc cgctggtagg caagctatag atattgagaa tagactgaat
     3661 caatggtttc aatctgtacc gaaacacttg tttaaatcca tcacatttga ttgtggaaaa
     3721 gagttctcga attggaaaaa gattagtaat accaatgata tttcgattta tttcgctgat
     3781 ccaggaacac cttctcaacg aggactaaat gaacattcga atggcttatt acgtaaagat
     3841 ggcttatata aagctatgga ttttaataag gtatccgaaa cctttatcca atcggtggca
     3901 tcaaaacgaa atcatattcc tagaaaatca ttacactatc gcacaccatt ggaagtattt
     3961 ttgagttacg taggtaaaga tgatttgtct agcttaattt gacaaacgaa aaatttatta
     4021 tatacttgaa ttgttcatta atgaaagcga aaccaatcgt tagcaactat gtaaaagaaa
     4081 ggaagattct tgtgaaaaaa agtattttgc ttatttctca tagtcaaaaa gtaacagatg
     4141 gtattaaaga aatgattgag cagatgcaaa aatccgaaga ggtgcaaatt ttttctctcg
     4201 gcggaataaa tggcgagatc ggatcagacc cgatgaaaat cgtagaagca gtcaatgaat
     4261 caagcgatgc agctagttat tttgtttttg cggatatcgg tagcgcggta ttgaactcgg
     4321 aactggcaaa agacatgttg gaagaagagc agcagcaacg ttatcatctg gtggatgcgc
     4381 ctttagtgga aggagctttt gcagcagcca tcactgccgg tataacagat gatactcagc
     4441 aaatcttaac agaagcacaa aatgccggaa agaaaggatg ggaataaatt gaaaaaaatc
     4501 atgaatgatc caagcaatat tgtcgaagaa atgctggaag gattagtaaa aagctatcca
     4561 gaacttgttc atcgtgtaga atcttcccgt gttgtagcca agaatcagaa agcggaacaa
     4621 gttggtttgg tttctggtgg aggaagcgga catgaaccat cccatgccgg gtttgtaggg
     4681 gaagggatgt taagtgcagc ggtgcttggt gatgtgttta cttctccaac tccagatcag
     4741 attcagacgg ctattaagga agccgatagt ggaaaaggag ttttgctgat cgttaagaat
     4801 tatacagggg atgcattgaa cttcgatatg gcaaaggaat tagctgctat ggacgatatt
     4861 gaagtagaaa gtgtgatcgt agatgatgat atcgccgttg aaaatagtac gtatactgct
     4921 ggaaaacgcg gagttgcagg tactgtcctt gtacacaaaa tcgtgggcga tgcagcaaga
     4981 aacggtgctt ccctagctga gttgaaagaa ctgggagaaa aggtcgtaca ggcaactaag
     5041 acgatcggtc tagcgcttcg agcagcaact gtaccagaag tagggaaacc aggattcgaa
     5101 ctaggagaag atgagatcga gtatggtgtt ggtatccacg gtgagccagg ctatcgtcga
     5161 gaaaagatgc agccttctaa agtactggca aaggaacttg ttactaagat tttagatgat
     5221 tactcgaatt taccaaaaga agcaggtgta ctagtcaacg gaatgggcgg gacgcctttg
     5281 atggaacagt tcgtgttcat gaatgatgtt ttagctctgt tggaagaaag aggcgtcaat
     5341 gtagttttcc gaaaagtcgg taattttatg acctcactag atatgcaagg tctttctcta
     5401 acactgatcg acttgacgga gacacaatgg aaagattctt tagaaagcaa tgtacagacg
     5461 atttcatggt aaaaaatctc aagataaaag ggtgataata tgaaactaac cgttcaagaa
     5521 atccaagaat ggctcacgca atttgcagag gcaatcaatc agaacaaaca atatctcagt
     5581 gatctagata ccccgattgg cgatggagac catggaaata acatgggaag aggggtttca
     5641 gcttatgaag aagcttttca agccgatcat cctgaaacaa tcagcgatac attcaaagta
     5701 ttctctatgg cgatgatatc gaaagtcggt ggggcttctg gtccgttata cggatctgca
     5761 ttcatgaaca tgatgaaagc gacaaaagag gtcgattcta ttgattctca agaaaaatta
     5821 ggcgaagtga tcgagcaggg aacagcgggt attcaaagcc gaggaaaggc agaagctgac
     5881 gataaaacga tgttagatgt ttggctgcca gtcacagagg cattgaaggc agggaattta
     5941 acaaaagaaa tcattgacca ggcaaaagaa cacaccaaag tcttggtagc taaaaaagga
     6001 cgagcttcct atctaggaga gcgtgcaatc ggacatatcg acccaggagc tgcttctagt
     6061 gccattttat tttcaacgct tttagatgtg atcaatcaac cttaagttca ataaa
//
LOCUS       DAZUMK010000138         6052 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000138 DAZUMK010000000
VERSION     DAZUMK010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.141"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            164..1114
                     /gene="yidC"
                     /locus_tag="ACG808_002534"
     CDS             164..1114
                     /gene="yidC"
                     /locus_tag="ACG808_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFS5048126.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(1293..2267)
                     /gene="lacD"
                     /locus_tag="ACG808_002535"
     CDS             complement(1293..2267)
                     /gene="lacD"
                     /locus_tag="ACG808_002535"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFS5048127.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPECGLPAAALREKECGLLLAYEKTGYDTKMPGR
                     LPDLLNIWSVKRLKEAGADACKFLLYYDVDEPAEINVQKQIVIERIGSECDAEGIPFF
                     LEILSYDATIGDPSSLAYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QETCYSQAEASAYFYEQSKVTEQPFIFLSAGVNATLFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLKETATPVYVDR"
     gene            complement(2264..2722)
                     /locus_tag="ACG808_002536"
     CDS             complement(2264..2722)
                     /locus_tag="ACG808_002536"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFS5048128.1"
                     /translation="MTIIHEENIFLQQPLDSQEEAFKFLAKQTFCLGIAHDEKEVYRK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSMIIVTLNKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRKAKSKTEIATILTKEIGEDL"
     gene            complement(2719..4182)
                     /locus_tag="ACG808_002537"
     CDS             complement(2719..4182)
                     /locus_tag="ACG808_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFS5048129.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFAGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTILADNEAVDTLESETISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSFDPDHAQYNATAAMLKTVGDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     SGGTGFLVGILAGFLAGYFILFLQKLLTILPKQLDGLKAIFLYPVIGVVFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEHSNPLVLGVIVGCMCAFDMGGPINKAAYVTGTALLAQGN
                     TTFMAGVSVACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKMDNKAANRTVIEEGRAGGLS"
     gene            complement(4164..5114)
                     /gene="pfkB"
                     /locus_tag="ACG808_002538"
     CDS             complement(4164..5114)
                     /gene="pfkB"
                     /locus_tag="ACG808_002538"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFS5048130.1"
                     /translation="MSIYTCTMNLAIDLFIETEEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQKKQIQTEFIEVPGMTRINVFTQVNQTREE
                     YKLVNQGPEIPQAAVHNFLNQIRALQAEDYLCVSGSLPRGMSPKVLVEISRICQEKEI
                     HLIIDSSDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENV
                     LLSLGEKGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLVGYMNRRPLDETLRKS
                     IAAGSSTAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            5257..6005
                     /locus_tag="ACG808_002539"
                     /pseudo
     CDS             5257..6005
                     /locus_tag="ACG808_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
BASE COUNT         1838 a         1327 c         1037 g         1850 t
ORIGIN      
        1 ttctttccaa gtatttcttt cttgattttt tgtctttttt caaaattttt cgctacgaaa
       61 aagaaagaat atttatatga aatttctatg aagttgattg aattccatgc gcttttttag
      121 tatagttaag tttgtgtaaa aattatagaa gaggaagtat tctatgaata aatggaaaaa
      181 ctggttattg ggatccggac ttgtcagtat cctgctcttt ttatctggct gtgtgcggat
      241 ggattcaaac ggaaaccctg acacttcagg aatcgtttat cgagtactcg tccatcctat
      301 gggccaagcg attacatatc tagttgagaa tttcaattgg tcttacggct gggcagttat
      361 tgcaatgact gtgatcgttc gtatcatcat cttgccacta ggtatcagtc aatcgaaaaa
      421 aacaatgatc caatctgaaa aaatgcaagc gttaaaacca caagttgaag cagcacaaca
      481 aaagctaaaa gctgcaacaa cacgtgaaga acaaatggca gcacaagcag aaatgcagca
      541 agtatatcgc gaaaacggat tgagtatgac cggcggaatc ggctgtctgc ctctattgat
      601 ccaaatgccg atcttctctg ccttgtattt tacggctcgc tacacagaag gtatccgtga
      661 atcatctttt tacggcatcg atctaggctc accaagtatg gtgcttgtgg ccattgcagg
      721 ggtcgcttat ctgcttcaag gatatatttc aacaatcggt attcctgaag aacagaaaaa
      781 gacgatgcgt acgatgctga tcgtctctcc tgctatgatc gtcttcatgt cgatcagtgc
      841 gccagccggc gttacacttt actgggtagt cggtggtgtg ttcagctgtc tgcaaacatt
      901 catcacaaac gttatgatga aaccacgctt gaaagcacaa gtggcagaag agttgaaaca
      961 aaacccgcca aaacaagtcg tcactccaag aaaagacgtg acagaatcag caaaaccagc
     1021 tgacaacaaa caagcaacaa aacaaatcaa acaaccaaaa aacagtacaa ataacaaagg
     1081 ccgcaacgca ggaaagcagc agaagcggaa gtagatagta aataagagtg taataaaaat
     1141 tcattaacag ttttagcaat taaaaatatc gaccaagatt tttgaaaaag aaatctagtc
     1201 gatattttta tttaagtgaa tgtataattt gaatcatcga ttgatcgcta tctaattgca
     1261 gcagcatctt tttatgaaga aacaactttt tcttatctat caacgtagac aggtgttgct
     1321 gtttctttta atagtgtact taattcctta atattttgtt ttccttcttc taacatccaa
     1381 ctactcgctt tttcttcgcc ttcggtaaca aaaggtgcca ctcccttcgc ccatgttgct
     1441 cgcccacata agacaccatt gaatgtcgaa cctgattgtt ttgcaaaacg taatgtatct
     1501 tgaaataacg tagcattgac tcccgcactt aaaaaaataa acggttgttc agtcactttc
     1561 gattgttcat agaaatacgc ggatgcctct gcttgactat aacaagtctc ttgcccatat
     1621 ccttccacaa atctcatatt cactggcact tctactttca atacatccac ttgataacgt
     1681 tcttttgaaa attctcgcat ggcttcaata actttatggg gttttacttt tgcataggca
     1741 agagaggacg gatcgccaat agttgcatca taagaaagga tctctaaaaa gaaaggtatg
     1801 ccttctgcat cgcattctga accaatccgt tctataacta tttgtttttg cacattaatc
     1861 tctgctggtt catccacgtc atagtacaat aaaaatttac aagcatccgc accagcttct
     1921 tttaaccttt tcaccgacca aatattcaat aaatcaggta atcttcccgg cattttcgta
     1981 tcatagccgg ttttttcata agctagcagc aagccacatt ctttctctct aagcgctgct
     2041 gcaggcaggc cgcattctgg atcaagtaaa atagctgagg catagggagt caggtattga
     2101 gaaaccatct gtttaaattg gatgatgtct tctgatgtag cttctttata ttggttgatc
     2161 attttcttca aagcaccacg ctggtcgatt gctaacgcgc taataattcc agcttcatcc
     2221 gtcaatcgct tcaaacgttc ctgcttttct atactcatct tcttcataga tcttcaccta
     2281 tttctttagt taatattgtg gctatttctg tttttgattt tgcttttctc aactgttctg
     2341 taacctcttt tcgcatcaat gatcgtgcaa cgagagacaa taatttcaaa tgagtcgttc
     2401 ctgcttctga atctggaata aataacgcaa aaataaaatc aactgactgt ctatctaaac
     2461 tgttccattc aattcctttg ttcagtgtga cgataatcat actaggttcg ttgattgttt
     2521 gagacttcgc atgagggata gcaaaacctg acattattcc tgtagtacct tctttttctc
     2581 tttctagaag ttttcgatat acttcttttt cgtcatgagc gattcctaaa cagaatgttt
     2641 gctttgctaa aaacttaaat gcttcttctt gactatctaa tggctgttgt aaaaaaatgt
     2701 tctcttcatg aatgattgtc atgataagcc tcctgctctt ccttcttcga taactgtacg
     2761 atttgctgct ttattatcca tcttcttgtc tttatcagtt actcttttct tttggatcaa
     2821 acctagtatc actccaccaa cgatcgaacc aataagaatc gctgtaaccc attggatttt
     2881 tcccgtcact acaggtaaca ctaaaaaacc gccatgtggg gctggtactt ggacaccaaa
     2941 cagataggtc aagacagcag caattgatga gcctaacatc atcgttggta atactcggat
     3001 cgggtctttc gcagcaaacg gaatagctcc ctccgtaata tgagttgatc ctaaaataaa
     3061 attaaccaaa cctgcattac gatcattagt atcaaaatat ttaccaaaca ataaagttgc
     3121 aaaaccagta atcaacggtg gtgcaatgca agctactgac acgccagcca taaaagtcgt
     3181 gtttccttgt gccaaaagag ctgtacctgt tacatatgcc gccttattaa tcggtcctcc
     3241 catatcaaac gcacacatgc aaccaacaat cacgccaaga acaagtggat tcgaatgttc
     3301 aaatccagct aaaaagctca tcattccttc atttatagcc ttcattggac ccgccagcaa
     3361 agacatcacg atgccgataa acactacacc aataactgga tataagaaga ttgccttcaa
     3421 gccatctagt tgtttaggta aaattgtcag tagcttttgc aaaaataaga taaaataacc
     3481 tgctaagaac ccagctaaaa tacctactaa aaatccagta ccgccactca tggccacgat
     3541 tccaccaaca aatccaacga ctaaccctgg acgtttcgcg atcccttctg caatataagc
     3601 tgctaaaact ggaaccatca tccccattgc tgcatcgcca accgttttta acatcgccgc
     3661 tgtagcattg tattgtgcat ggtctggatc aaaagaatag attccccaaa cggcaaatga
     3721 aatagcaatc aatactccgc cagctaccac gaaaggcaac atatgagaga cgccattcat
     3781 taaattttta tagatgctgc gaccaaaatt agcaccactt atcgtttctg attctaaagt
     3841 atcaacagct tcattatctg ctaagatggt cccttttcct gccaatgctt cgtcaatcag
     3901 ccgttgtgca tccttgattc ctgtactgac agaaacatca atgactcgtt ttcccgcaaa
     3961 tctttctgaa tggacatctt tgtctgcggc aataatgaca gcatctgcct gtttgatttc
     4021 ttcagccgtt aattcattct cgatcccaat ttgtccatgg gtctccactt tgatcgtgat
     4081 gccttttttt ctagctgctt gctctaatgc ctcttgcgcc atgtaggtgt gtgcaattcc
     4141 tgtcggacaa ccggttgctg caattagttg atattttccc atctttcgaa cctctcttcc
     4201 ttgattttga tttgtttact taattcctcc acatccaaaa aatcagtcag tcctttacga
     4261 aaagcagtag agctgcctgc tgctatgctt ttacgtaacg tttcatcaag cggtcgtcga
     4321 ttcatgtatc ctactaaaaa agtgcctaac atggcatcac ctgcacatgc tgtattgaca
     4381 acttttcctt ttggcgagtt tcctcgaatc accctgtctt tcgtaaaaag aatcgctcct
     4441 ttctcaccaa gggataacaa aacattttct gcacctgact ctaccagtcg ttgtccataa
     4501 acaaagtaat cttcatctgt aaccattttt cgtccgaacc aagaagaaag ttcttcctca
     4561 ttcggtttca gcaaaaatgg tcgatagggc aaacaatcca tgacctcttg atcactgcta
     4621 tcaataatca aatgaatctc cttttcctga caaatccgac tgatttccac taaaaccttc
     4681 ggagacattc ctcttggtaa actcccagaa acacaaagat aatcctctgc ttgtaatgct
     4741 cttatttgat ttaaaaaatt atggacagct gcctgcggta tttcaggtcc ttgattcact
     4801 aatttgtatt cttctctcgt ttgattgact tgtgtaaata cattgatacg cgtcatacca
     4861 ggcacttcaa taaactctgt ttggatttgt ttcttctgta aatagtcttc tatatatttt
     4921 cctgtgaaac cagcagaaaa acctaatgcc gtacttggga aatctagcat ctttaatact
     4981 agtgacacat ttactccttt tccgtttgcc tgaatgtcat cctcctttgt acggtttacc
     5041 ataaaaggat gcatctcctc tgtttcaata aatagatcaa ttgccaaatt catcgtgcat
     5101 gtgtagatag acataaccaa tgacctcctt gaatctatta tgaaatacgc gaaagagaaa
     5161 atgttttctt aatcgttcca acacaaagaa aacctttaca tttataaaca catcgtgcta
     5221 ttttttaact aacttttagt gaaagaagga aagaatatgg atcacaaatt aagtgaaaca
     5281 gaacagtacc tctggaactt tatcgaacac cacatcctag agatccctaa ctactccatc
     5341 gtgaaactaa gtgaacaagc aaatgtctca acagccacca ttgtgcggac aatgaagaaa
     5401 aaaggctatg aaggatttac ttcgtttaag caccatctaa aagaaggaga aaacaagaac
     5461 atcaattttt ctttttagat aaagtagaca aaggtatccg tcgagccatt ctaaaaaatg
     5521 aacaagaagt ggtacgtacg atcaatatgt tggaaatcgg gaacattgaa gatgcaatac
     5581 aaagaatcaa atttactgac cgggtttttg tctttgcccg aggattttca gaaatgattg
     5641 gtcaagagat gctcgtaaaa cttcaattaa caggaaaaaa ttgtcaaatg catactgatc
     5701 cagagattat caaaagcatc agtcggaaac taactaagaa agacagcgtt atttttgttt
     5761 ctttgaacgg cgagacaaaa gaactcgtca ccgccgcaaa gaactgctat gatgcagaga
     5821 ttggttcgat tctagttaca gccaatcatt cctctacact caaagattat tgcgagataa
     5881 atctcgtagg tttcaaatca gaaggttctt atttccctga ttatgaagta cgttcacgct
     5941 tgcctctcca gatcatcgcg agaatcctgc ttgacgccta tgtattgcgt atgagggaga
     6001 attgagaagc agtttattat ggttggttac agtttaaaac atcacatttt tt
//
LOCUS       DAZUMK010000139         6005 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000139 DAZUMK010000000
VERSION     DAZUMK010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.142"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            378..1067
                     /locus_tag="ACG808_002540"
     CDS             378..1067
                     /locus_tag="ACG808_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS5048131.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1054..2262
                     /locus_tag="ACG808_002541"
     CDS             1054..2262
                     /locus_tag="ACG808_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFS5048132.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2556..3827
                     /gene="serS"
                     /locus_tag="ACG808_002542"
     CDS             2556..3827
                     /gene="serS"
                     /locus_tag="ACG808_002542"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFS5048133.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4376..5860)
                     /gene="guaB"
                     /locus_tag="ACG808_002543"
     CDS             complement(4376..5860)
                     /gene="guaB"
                     /locus_tag="ACG808_002543"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFS5048134.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
BASE COUNT         1839 a         1135 c         1163 g         1868 t
ORIGIN      
        1 aacgactcag ttatcttatc atgtcatttc caacatgtca acaacttatt ttttattatt
       61 taataagtta ttggtgttct tttgaacaaa taataatata tcaacatcac atgtgaaagt
      121 caataagaaa atgcttattt atcaacattt ctagcttaca tgatcattgg ttagttctgg
      181 tcctttttcg ttcgtcttta taactccgag agacgtaaaa tccttttgtt ttcagtttaa
      241 tgttcatgtt cttgtcttat tcacaccaac tagatgtctc accttctaaa attttcctta
      301 acaaagtgct gcttttatct atttctgctt ttttttgcta gaataatagt aacaatatga
      361 ctctggagga tttatttatg aaaattttga ttgctgacga tgataaagaa atcgttgaat
      421 tattaagtat ctatgttcac aatgaggggt atgaagcagt aaaggcttat gatgggaaag
      481 aagcattatc taaaatcaga acaatccctg atattgaatt acttatttta gatatcatga
      541 tgcctgagat ggatgggatg caagtagtga aagagctacg aaaagaatcc caaataccaa
      601 tcattatgct gacagcaaaa actactgata tggataagat caaaggactg gtcgctggtg
      661 cagatgatta tgtgaccaag ccatttaatc cgttagaagt aatggctaga gtaaagtctt
      721 tattacggcg tacaaaaatg cagattgccc cagatcagcc tgatgtatta gaagtaaatg
      781 cattaatcat caataaagac tctcatgaag ttaaaacaat cgatgggaaa gaaatccagt
      841 taacagcttt ggaattcggt atcctctact tgcttgctag tcatcctaat cgagtattta
      901 gcgcggatga aatttttgag cgtgtttgga aacaagaaag tattgtctct gctaaaactg
      961 tcatggttca cgttagtcat ttaagagata agattgaaga agcaactggt ggtgaaaaag
     1021 tcattcaaac cgtttgggga gtgggctata aaattgacgc aagatagtaa acgagcaact
     1081 gaaaaacgcc gtcggatcaa tttgacttca aaagaaatca gcgaattgtt agcggaagga
     1141 atcatcacga ttatcctttt gcttctatta aatgtttcta ttctggttgt tatcagttcg
     1201 gtgatcaaca gttcaccttc tttgacgaat gcgatttggg attcaaagaa tatttttgca
     1261 gaacgattga acactgattt gttctggaac ggccggaatt ttattattcc ctttttcttt
     1321 ctattagata ttggtgtttt gtattggcgc ttgattcgaa gatatcgcca aatgcaattg
     1381 cggcatatca tcagcgaact tcactacatc gcaaacggaa attatgatca tcgtatccct
     1441 tttgaactga gtggtgactt aagccgagtg gtaacaagta tcaatggatt ggtggatagt
     1501 accgtagcag cgattgaaga cgaacgtaaa atcgaaaaat caaaagatga attgatcacg
     1561 aatgtcagtc atgatatccg taccccactc acgtccatca ttggatacct aggactgata
     1621 gaagatggcc agtatcactc agaagaagat cttttaaagt atacgcatac tgcttatatc
     1681 aaagcaaagc agatgaaatc tttagtggac gacttatttg aatacactaa ggtacgccag
     1741 cctgctgtcc cggtgaattt ttctgccttt gacatgatcc agttgatcga acagttagca
     1801 gctgattttg agttggaagc ttctaaaaaa aatatccaaa tacttgttca atcaaaagta
     1861 gactccctta tcatggatgg cgataccgaa aaacttgtac gtgtcttcaa taatcttttg
     1921 acgaatgcgt tgaaatacgg aaaaggagca acgaagatcg taatcgaagt agaacgtatc
     1981 ggttcagaag ttgttgcaac agtcaaaaat aacggagcaa tgatcccaca acaggcaatc
     2041 gataatctat tcgaccgttt ttatcgtgta gaagaatctc gttcacaagc aactggagga
     2101 actggacttg gtttagcaat cgctcaaagc attgtggctt tgcacggcgg atacatttat
     2161 gcaaaatcag ataagcaatg gacttctttc atcatgcacc ttccaatcaa gaaaaatgaa
     2221 aaattaccta ttgatacaca ggaagttatt gacgaaagct gaaatattcg ctattcttat
     2281 tacaattatt agaataaatg aaaaacacag aacccagtac actttattct ttttcagaga
     2341 gctgatggca ggtgtaaatc agcaaaggat aatttggaag ttccagttcg tttacgtgcc
     2401 ctttcgaaat aagaaaggtc ggttcaacag ccgttatgtg ttcaacaagt gcagctgacg
     2461 ctgaacttgg gtggtaccgc gaatttcaga ctgattcgtc ccaagaataa tgggatggat
     2521 cagtcttttt ttattttcaa aaaggaggaa atcatatgtt agatgtaaaa atgatgcgac
     2581 aaaatattga agaagtacaa gaaaaattga tgactcgggg cgtaaaaaaa gaaacattag
     2641 ctgaattcat ggaactagat gaaaatcgtc gtaagctact agtcaaaagt gaagaattaa
     2701 aaaaatatcg aaatgatgtt tctgctgaga tcgcccaatt gaagcgtgca aaagaagatg
     2761 cttctgctaa aatcgctgaa atgaaagaag tcggtggcaa catcaaagca ttggatactg
     2821 aaattgctga agtggatgaa aaattgaaac aaattgcaac tactttgcca aaccttcccc
     2881 atgattctgt gccagttgga acagatgaag aggacaatgt agaaatccgt cgttggagcg
     2941 aaccacgtac atttgctttt gaaccaaaac cacactggga aattgcagaa aatcttgata
     3001 ttttagattt tgaacgtggc gcaaaagtag ccggtagccg ttttgtctat tacaaaggat
     3061 taggtgctcg attagaacgt gctgtctaca acttcatgct tgatcagcat atttacgaac
     3121 acggctatac tgaaatgatc actccttata tcgtcaacag taaagcgatg tttggtacgg
     3181 gacaatttcc aaaattcaaa gaagatgttt tccaattgga gaatactgat ctaacattaa
     3241 tcccaacagc tgaagtacca ttaactaact attataatga tgaaatttta gatggtgcag
     3301 atttacctat ttatttcact gcactaagcc ctgcttttcg ttctgaagcc ggaagtgctg
     3361 gtcgggatac acgaggactg attcgtcttc accaattcaa taaagtagaa atggtcaaat
     3421 tttcggatgt agagcattct tacgaagaat tggaaaaaat gacagctgat gcagaagata
     3481 tcttgaaaaa attgcatttg ccttatcgtg tgatggcctt atcaactgga gacatgggct
     3541 tttctgcagc taaaacttat gacttagaag tatggatacc tgcacaagat acttatcgtg
     3601 aaatcagttc ttgttctaac tgtgaagatt tccaagcacg ccgcgcaatg atccgttatc
     3661 gcgataatga tggaaaaatc cactatactc atactctgaa tggttcagga cttgcagttg
     3721 gccgtacagt agctgccatt ttggaaaact accaaaatga agatggttct gtcacagttc
     3781 cagaagcgct cgttccttat atggggaatc tgaaagttat taaataaaga ggctgtgaaa
     3841 aagccgctta gtttcgagta ttaagaaaaa ttttgtgaaa atggctttcc acatttttgc
     3901 ataaattgga cttaataccg agaaactggc ttttgaacac catttatcga gaggttgtga
     3961 aatcagcgaa ttaacctgag tattagagaa aaaaatggaa aaatagtccc tcatatttta
     4021 ccgtttgttg aactaatacc gagggtcagc tgattgaaca ccgtttagtg gagaggtcgt
     4081 aagcctaccc cccagctcct agttacaagg acaaattcaa aaaacagctc ttcatatttt
     4141 atgaatgttg tacttgtgat tcggaggtgg caagggaaca ctgtttattc aaaaagaggc
     4201 tgggacagaa gcgtttaact ccaagaaata agaagaaatt cacgaaaatt gcttttcaaa
     4261 tttttgtgaa tttcttctta tttccgaagg agttgcttct acttccgccg tttatacgtg
     4321 tttagagcgt gagacaaaag tgttctttac ttttgtccca cgctctttaa tgtttttaag
     4381 attcaactga atagtttggt gcttcttttg tgatttgtac atcatgaggg tgtgattctt
     4441 ttaatccact gccgctcatt tggataaatt gcgcttcatc acgtaattgc tgtagattgg
     4501 ctgccccaac ataacccatt cctgaacgca aaccgccgat catttggaaa ataatatctg
     4561 caacacttcc tttataagca acccgacctt cgattccttc tggtactaat ttatttgctt
     4621 cattgacact accttggaag taacggtctt ttgaaccttt ttccatcgcg cctaatgagc
     4681 ccatacctcg gtatgttttg aatcgacgtc cttggaagat ttcgaattca cctggtgatt
     4741 catctgtccc tgccaacata cttcctagca tcactgcatg tccgccggca gctaatgctt
     4801 tgacgatgtc accagaatat ttgatccctc cgtctgcaat aatcgctttg ccatattgac
     4861 gagcaactga tgctgcatca taaatagctg tgagctgagg tacacctaca ccggctacca
     4921 cacgtgtcgt acaaatcgat cctggtccga taccaacttt tacgacatct acacctgcat
     4981 catataaagc ttttgtcgct tcagccgtcg caacgtttcc tgcaatcaat gttgcatctg
     5041 caaaagttga acggatttct tgaatttttc ggatcacacc tgcactatgt ccatgagctg
     5101 tatcgatgat gattgcatct gcccctgctt ctaataaagc attcgcacgc tcaaatgtat
     5161 cgcttgttac accaacggct gctgctacaa gcaatcgacc atgttcatct tttgctgcat
     5221 ttggaaattc gatcactttc tcgatgtctt tgatcgtgat caatccgctt aaacgacctt
     5281 cgttatctac gattggtaat ttttcgattt tatgtttttg caaaatcttt tctgcatctt
     5341 ttagagaggt acctactggg gcagtcacta agttgtcttt tgtcatcaca tcagcaatcg
     5401 gcatgtgata atctgttaca aaacgcatgt cgcggtttgt aattatccct accaattttc
     5461 tgttttcaag tgtttcaacg attggcacac cactgatacg atatttgctc atcaattctt
     5521 ctgcgtctgc tacaagatgc tgtggcgtta agaagaacgg atcaatgatt acgccacttt
     5581 cagaacgttt gacttttctt acttcatccg cttgctggct gatcgtcatg tttttatgga
     5641 tcacacctag tccgccttgt cttgccatag caatggccat cttgctgtcg gtaacagtat
     5701 ccatacttgc actgatgatt ggaatattca acttaatatt tttagctaat tgtacgctca
     5761 tatccacttc gtttggtaac acgtgacttt ctgctggtat caataagaca tcatcaaaag
     5821 taaatccttt tttcgtgaac tttgtttccc agttggacat ttttagtacc actcctctaa
     5881 tttagtttat ctattaaaat aattgatttt ttcatttaag tcaatgatct acttggaatc
     5941 aagttagctt tctcatagaa taccaatata aagcgtttac atctgtgtat attaaaagag
     6001 attgt
//
LOCUS       DAZUMK010000140         5974 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000140 DAZUMK010000000
VERSION     DAZUMK010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.143"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(74..1168)
                     /locus_tag="ACG808_002544"
     CDS             complement(74..1168)
                     /locus_tag="ACG808_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFS5048135.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1357..2118
                     /locus_tag="ACG808_002545"
     CDS             1357..2118
                     /locus_tag="ACG808_002545"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS5048136.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2233..2673)
                     /gene="fabZ"
                     /locus_tag="ACG808_002546"
     CDS             complement(2233..2673)
                     /gene="fabZ"
                     /locus_tag="ACG808_002546"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFS5048137.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            2893..3648
                     /gene="fabI"
                     /locus_tag="ACG808_002547"
     CDS             2893..3648
                     /gene="fabI"
                     /locus_tag="ACG808_002547"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFS5048138.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3758..4810)
                     /locus_tag="ACG808_002548"
     CDS             complement(3758..4810)
                     /locus_tag="ACG808_002548"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFS5048139.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            4936..5856
                     /locus_tag="ACG808_002549"
     CDS             4936..5856
                     /locus_tag="ACG808_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFS5048140.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1824 a         1194 c         1074 g         1882 t
ORIGIN      
        1 tataaaaaaa caaaataaaa aagcagaaat aactggctat ttctagcgga ttatttctgc
       61 aataactaat tatctatttt ttattttcaa ttaatttaaa gcttgaccat ggtttcaaaa
      121 agtcaaggag gaataattcg tcatcggaga ttcttcctac aacattgaca cgtccgtcgt
      181 ttttcatatc cttcaaggcg atttgtgttt ctcctttgta ttgaccgtac ccttcattgt
      241 caatcagtac atccccatga tgcatatctc gcgtattatg aggcgggaaa tctttatctt
      301 tgtagtatac acgagtcatc gttgaacgaa ggatatattc tgagcgatcc cctcgataac
      361 tatgaaggtt atcaaacaag cagattcgtt cgttttctgt aataccttct tccgtatcta
      421 ctttcaatgt tggataatcc gcgttaaatg cttctgccat ttctttcaat tcttcttcac
      481 ttgcgtacgc atttccaata gaaatatcat caatcaatcc tgtaaggatt aaatgcttca
      541 cttgtgtcgc aatttcaaga tctcggtggt cttccagcgt gcacaagcca tcttgcgtag
      601 gccaagggcc aaactcagca gattgcgaat tgacgaatgc catcgtattc aaattatatt
      661 ttctgaattt ttctgaacaa aaaacaaagt gttcgtatcc taaaccagag tatcgatgag
      721 gataaaaatt atgagaacct aataaattat ccgtgtttgg cgaataactc atgatgttgt
      781 caacgtaatt cgtgcccgaa ctcatattga tctcgatttt gataccaaaa ggattgcgcg
      841 tcatttttgc ttcttctgct cctgtgaatc cgatatctaa gcggaccccg taggcaccca
      901 tcttatggaa aaaagataag tcatcgtaag aaatctccaa ctgttcaaat aacgctggat
      961 tgatatcaac cattacttcc atgcctaggc tattcgcgta atcaactact tctttaaatt
     1021 ctgaaagaac tttttcttta tcatcattga tctgcagcaa actggtaaaa acccgtttat
     1081 acccatactt atgagctaaa tccaagtacg ctttgtcctt ttcaaatgtt gaccgttctg
     1141 gataaatcga gattcctaat tttcccatat taccatttcc tcctgcatct tttcgttttt
     1201 gtatccactt acattatagc aaacacaaag taaatggtct acaccttttc tttttctttt
     1261 aaaataaatg ataaattcca ataatttaag taggtatttt ccctctattt tgttatcatt
     1321 aaagataaca aaatagtaaa ttggaaagga acagttatga agaaaaataa agttatttta
     1381 agtattagtt tagcagcagc attgctgttt gctgctggtt gttctgcaaa caacacgact
     1441 tcttctagtt cgtctacaga aagttcaaaa agtacatcta ctgcggaaag cacgactgcc
     1501 agtagcgtcg atttgaacgc attggaatta ccacaattga cgaaagatgt cgccgaaaat
     1561 gaagatctgg tcgaaatggt gacgaataaa ggcacaatca aaatcaaatt atttcccgaa
     1621 cacgcaccaa aagcggttga aaactttatg acacatgcaa aagacggtta ctacaacgga
     1681 ctgactttcc atcgcgtcat caaagatttc atgatccaag gtggcgatcc taaaggcgat
     1741 ggcactggag gggaaagcat ttggggagaa ggatttgaaa cagagatcaa caaccacctt
     1801 tataatataa gaggtgcgtt atctatggct cgctcacaag atcctaacag taacggcagc
     1861 cagttcttta tcgttcaaaa tacagatgat atgcatgatg gtcttttaaa agacgactat
     1921 cctcaagcaa tcatcgacgc ttataaaaac ggcggttatc cttcactaga tggccattat
     1981 actgtcttcg gccaagtgat tgaaggaatg gatgtcgtgg atgctattgc aaatatcgaa
     2041 acagatagtt cagacaaacc aaaagaagaa gtaaaaatcg ataaaatcaa tatactgcaa
     2101 gaagcaaaac aaaaataaaa aaataacgtc tgaatcctgc atagatgaca acgtatgcta
     2161 ggattcaggc gtcttctttt tacataaaaa gcaagtaagc ttccttttgt tttgctatat
     2221 caggttttat agtcaagcat tttggttgac gatgaacgtg aactcgcact cacagacttt
     2281 tttatcatct acatatgctg cagataacgc tgtaccgact gatcccttca ttttggtgat
     2341 ttcaaaatga catttcagca catctcctgg aacaactttt tgacggaatt tggccttatt
     2401 gatcccaccg atataagctc tttttcctag aaactcttct gattttagga taagtatcga
     2461 acctgcttgt gccagtgttt ctagaatcaa cgcacccggc atcactggat tgtcaggaaa
     2521 gtgaccttgg aaaaaatctt cattgattgt tacatttttt gtcgcaacga tcttttttcc
     2581 tggttctaaa gtatctacat aatcaatata acaaatagga taacgattag gaatcaattc
     2641 cattacttca gtagcagata atacgtaaga cataattaaa ctccttcttt caggcaattg
     2701 ccattttcat tactttgact atcaaagtat catattccta aatatttagc aatctttttt
     2761 taatttaaga aaaaatagtt tgacaatcaa aacattttga ctaataatta agaaatcaag
     2821 aagttatttt agctattatt ctctattttt ttacgactat caaattttta tcagaaacaa
     2881 aggagagtca ttatgtcatt tctagaagga aaaaaaatcg tcattatggg cgtcgctaac
     2941 aaaaaaagca ttgcttgggg ttgtgctgaa gcaatgttga aacaaggggc agaaattatc
     3001 tatacttacc aaaatgaacg aatgaaaaaa tcattagtca aattggtcgg cgaagaagca
     3061 ttcactgtag aatgtgatgt ttctagcgac gaaagcatcg aaaaagcaat gaatgccatc
     3121 ggtgaatatg ctggggagat ccacggcctt gtccatgcag ttgcttacgc caacaaagaa
     3181 gaattgtccg gaaatgtgtc tgatatttcc agagacggct atcaattagc tcaagatatc
     3241 agcagctact ctttaattgc tgtatcaaaa tacgcacaac cgtttcttgc accaaacagc
     3301 gggatcgtta gcatgactta tctaggttca gaacgcgcaa tcccgaatta taacatgatg
     3361 ggtattgcga aagcttctct tgaagctgct atccgttatt tggcagctga gttctcgcct
     3421 aaaggaattc gggtcaatgg aatctcagct ggggcaatca aaacattagc tgtcacaggt
     3481 gttaaagact atcaaaaact gatccagtta tctgaatctc gtacgcctga tcagcaaggt
     3541 gtgacgatcg aagaagtcgg taatgcttgt gctttcttaa tcagcccact ttccagcgga
     3601 atcatcggtg atattatcta cgttgacaaa ggtgtccatc tttcataaac ttttgttgca
     3661 ttctttaaag taaaacatat aaaaatcagg aaaaaacttt ttaaagtctc ttcctgattt
     3721 tttcttttta cgtttttttc aactcattac tttgaaatta ttccacgact tcaatccagc
     3781 cttctggtgc ttctatgtct ccaaattgaa tacctgtcaa ttcatcatac aaccgttttg
     3841 ttactggtcc tacttccgtt tcactataaa atacatgaag attgttattg tgataaatcc
     3901 caccgattgg agaaataaca gccgctgtac cacatgcacc agcttctgca aattgatcta
     3961 gatcatcaat gaaaatatct ccttcaattg cttgcagtcc tagtcgttct tcagctaact
     4021 gcaacaagga atatttcgtg atacttggta agatagaggg cgattgcgga gtgatgaatt
     4081 ttccgtcttt tgtaattcca aagaaatttg ctgcacctac ttcttcgatt ttcgtatgtg
     4141 tggctggatc aagataaaca caatctgagt aatccagttc ttttgcttct gttcctggaa
     4201 gtaaactcgc tgcgtaattt ccgccgactt ttgctgcacc agtcccctta ggagcagcac
     4261 gatcgtaatc agatacaata aaattcgttg gttttagtcc acctttgaaa tacgctccta
     4321 ctggcgtaca gaagacgaca aataaatatt ctggagctgg atggacacca atgttttcac
     4381 cgaccccaat cagtaaaggg cgcaagtata atgtagcgcc tgttccatag ggcggcacaa
     4441 aatcttcatt tgcttttacc acttgcttga cagcatcaac gaacatcttt tcagggacca
     4501 ttggcatcag caatcgtcta gcactttgat tcatccgtcg actattttgt tcaggtcgaa
     4561 agagattgac cttgccgtct tttcgacgat aagcttttag accttcaaaa cattgctgac
     4621 cataatgaag cgctggagag ccttcatgaa tcgtcaggta attatcttct gtcaattctc
     4681 cttcttccca ttgtccgtct ttccatttgg caataaaacg ccaaggagtt ttgatataag
     4741 aaaaacctag attgttccaa tcgatttgca tatacttacc accttcgtta ttttctccac
     4801 attttatcat tatccatatt atttgtcatt ttattttcag cttttttcct tcattatagt
     4861 tatttttgct atttcctttt ttgaaaactt cttggtacaa tacagaaaga atcaaatttt
     4921 agaaaagagg attttatgtt ttcattatta gcaactgccg gtacgacaga ctcttctggc
     4981 gagacattga cagatcaagc tgtcagcaaa gtaaatgcgt ttcagcggtt ttgggacggg
     5041 attgattggg acgccatttt ggctgccttt atccaaaaaa gtttatatct gatttttttg
     5101 atcgtccttt tttggattct tcaccgtgtg ggaaaatatc tgattgataa gtcttttaat
     5161 cagtattcta aaaaagccat tctaaacgag agccgcttaa agactttaca tagtctttta
     5221 aatactattt ttcattatac ccttggtttc ttttttatct atgcaatttt atctgctatc
     5281 ggtgtgccga tcggatcact tttggctggt gccggaattg caggtgtggc aatcggtctt
     5341 ggtgcccaag ggttcatgag tgatgttatt accggatttt ttattatcat ggaacaacag
     5401 atggatgttg gggactacat caagctagca aacttaacga ttgaaggaac cgtcgtatct
     5461 gttggtatcc gtacccttca gctgaaagca acggatggta ctgtccattt catcccgaat
     5521 cgaaacatca ccacgatcag caatctctct cgtgcaaata tgcaagtact tttagatatt
     5581 cggattgttc ctgaagaagg atatgacaaa atctatgaga tcattgatcg agtaaatcgt
     5641 acattagctg aaaaatacca agaagagctc caaactgaac cttctatctt tggattagtg
     5701 gatcttggaa acagcaattt tgcgattcgt acggtctgct acgcattgaa cggaaaacaa
     5761 tatgccttaa aagaagagtt cttgagctcc tatgtcaaag aattgacagc ttctggtttc
     5821 acgattccga acagtccaat tgctgtcgga aaataatttg gacaaacgat gcaacgaatc
     5881 tcactaaaaa gagcgtggga caaaagtaaa gaacactttt gtcccacgct ctaaacacgt
     5941 ataaacggcg gaagcagaag caactccttc ggaa
//
LOCUS       DAZUMK010000141         5952 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000141 DAZUMK010000000
VERSION     DAZUMK010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.144"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(441..1223)
                     /locus_tag="ACG808_002550"
     CDS             complement(441..1223)
                     /locus_tag="ACG808_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048141.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1288..2124)
                     /locus_tag="ACG808_002551"
     CDS             complement(1288..2124)
                     /locus_tag="ACG808_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS5048142.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2142..3551)
                     /locus_tag="ACG808_002552"
     CDS             complement(2142..3551)
                     /locus_tag="ACG808_002552"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5048143.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3565..5388)
                     /locus_tag="ACG808_002553"
     CDS             complement(3565..5388)
                     /locus_tag="ACG808_002553"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS5048144.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(5668..5895)
                     /locus_tag="ACG808_002554"
     CDS             complement(5668..5895)
                     /locus_tag="ACG808_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048145.1"
                     /translation="MIEIGYCLLIFTKKTSITIVAYIVGMLFIPSFTHQIHQFFPRAE
                     LLKLCDPLYAFLMLSRFWEFPAGYVGMVLLV"
BASE COUNT         1843 a         1280 c          966 g         1863 t
ORIGIN      
        1 aaaagcaaaa aaaaaaagaa agaaacttat tcttgctaat aaactacgcg aaaggagtct
       61 aacgttgttt gatataactt agactgatcg ttaacttatt ttttatattt ctacatagtt
      121 atctttagaa ccatttcaga tacatatgaa acaattacat atgatttttt cataaaaaaa
      181 cagtactgtc tagtagtata aaatatacaa atttcttaaa aattttttca ctaaaatcac
      241 tacctgaaac agtctaaaac acttatatat caacgtttaa tgaaactatc tccgtttcat
      301 ttcagataca aataaatata gtcgggctcc aaatccgaaa aaaaataccc ccagtagctt
      361 atcctaagct actggagatg tataagtacg gtcatataac aacggggtag ggtaaataaa
      421 tgaaaaagat ggaagaatca ttattcaata acttttttaa atacttcgtt gtaatcagtt
      481 acctgtccgt tcttaaatac cacagaataa tcttttttcc cattttcaag taaccgaagt
      541 ggaatgtctt taccaacttg ctctgtcact gcattactga tacctgttag attagctaat
      601 aaatctgata aagattgagc atctgtagaa atctctgtat cagtatttat cgtgattcct
      661 ttatcttcat ttacaagctt ccaagtaggt ggtatagctg tgtatgtttc gatgaatgaa
      721 tcaattcttt catctatcgt cttcccagta tctacttctg tctcattact cttagatgaa
      781 tcgattcttt catccatcgt ctgctccgta actgcttctg tctcattatt ctcaccggaa
      841 actctcttca cacccaaact tatatattgt ccatctttag tgtgcatagt taaaattccc
      901 acgcttttac taataaattc actataacca gttctttcaa tagagacttc aatgtatttc
      961 acactatcat actcaaaaga aaaatagaca tgtaaaagca ttttgttgtt tacatctaca
     1021 taataatcct ttgactctac ttgagacctt cctatacgga atacagcttt actgtctgat
     1081 atctctatac cagcatcctt attattaatc tgccaatctc caagtagctc ttctggcaag
     1141 ctagtagttt cttgtgaaca ctttaccact tcactggtag aggtcgaaac attcacctcc
     1201 gcactgttag aagaattttc caaggaatca ggagccttag cacaaccaac aagtctttat
     1261 atattgtgca gccgatattt tcttcattca gtacaccctt gaaatatgca gtgttagata
     1321 aagcagttcg tcctgatttg ctgaggcacc catctcttct tgaacgtact ccttgatttt
     1381 caaggccgcc cgatactctt tcggaaaacg ctgcttcact gaagccagca aaaaatcatc
     1441 cttctccggc aactcttcct tcttaatcat tcgggcaata aaaaactgta aatgcgtcac
     1501 aaatcttaag taaagcatcg aatctttatc aacctttttt tcgggaaaca gatatcctag
     1561 catagccaag atatccttta gtgctcttac cgagagcatg gtttcttcca tatttgcttg
     1621 ttcactttgg gcatttacaa tgtgaaacgc aatgtttcca gcctcctctt ctggcaactg
     1681 aacattcatt tttttgttga tcaattggat agcatagcag ccgatctcaa attcttttgg
     1741 gtaatacttt tgaatttcaa tcagcaggcg attttgaaaa acaatgcctt tttgactgcg
     1801 ttcaatggca tgcttcaagt gatcaaccaa ggtaaaaaat agacttttat tcagcttaaa
     1861 gtcaaacttg gcgttggcat aatcgataat tcgcttgacg caaataatta gatcatccgg
     1921 agcagcctca aacagtttaa tgtaatcctt ttttagagca tcagaaagtg aatctaattt
     1981 aaatactttt tggattttgt cagccggaac caaatcgcct ttctttagtt gaaatccaat
     2041 ccctttcccc atcaaaatta cttctttgcc ttgcttatct aagcaaagaa ttaaactgtt
     2101 gtttaacgtt ttaattactc tcattagatt caccgttctc tctaagctaa gccatttctt
     2161 gcgattactt tttgatacca aaagaagctt tcttttttta ttcgttttaa ttctttaaga
     2221 ttctcatcgg ttcggtcaac atatataaaa ccataacgct tattgaagcc ttgatgggta
     2281 ctaacaacat caatcgctga ccaaggtaaa taaccaaaaa catctacacc atctgttaaa
     2341 gctgcttgaa tagccagaat atgtttggct agataatcaa ttcgatcctg gtcattaatt
     2401 cgtctatctt cctcaactat gtcgggtgcc ccatagccat tttcagtaat taaaataggt
     2461 aaatcatagc gatcgtacaa tttcctcaat gttaatcgta gtccagtagg gtcaactacc
     2521 cagccatatg gcgttttatc tacataagga ttttcagctg cccggtagac accttcttct
     2581 cccaacatga tctgttgatc ccccgctcta gcggaaacat ctgatccgtc atttttgctg
     2641 gcagcgatgg tggcagttga ataataatta atcgcaataa aatcgggttt accggaggat
     2701 aaaattgcct gatcctctgc ctcaatttta ggatataatc cacgatcatt caaatagttt
     2761 tcaaacaaat aattgtattt tccccgaact gctacatcta agaaagacca gcctctcaat
     2821 gtttcccaat tatgggctgc aattgcatct tctggactgg aggtagcttg atacatagcc
     2881 gttaaattga ttgctggacc gatttttcct ttaagattca actgatgata caagtgaatt
     2941 actctagctt gagctaataa aacatggtga ttaatttgaa agagttcttt ttttgaaggc
     3001 agctctccac ttggtggcaa accaatcgct ccaggatgaa ggatcatcgt gttttgctca
     3061 ttaattgtca gccaatagtt caccttgtca ccatactcag taagcaatac ctgtacatag
     3121 tccacaaaat aatcgatagt tgctcgattt aaccaaccgc cctgctcctc tagaaaataa
     3181 ggtaaatcga aatggtacat ggtgacaatc ggttcgattc cctgattcag gcattcatca
     3241 attacttgat gataaaaaga aattcccttt tgattggttt tattattttc aatatccata
     3301 attcgcgtcc aagcgatgga aaagcgaaaa gcttttaagc ctaattcttt aaacagctgt
     3361 atatcttctt tatagtgatg gtaaaaatcc gaggcaactt caaaattagc taccccttct
     3421 gtatgctgat acttgtcaat aatagacggt tgtttcccat cctcctgata ggcaccctct
     3481 gcctggtagg cactaatgga ggcaccccat aaaaacttct ctggaaatgc tgaaagctgt
     3541 ttgtattgca caaaaatcct ctccttaact aatttgcatc aatggttcgt gttcctttaa
     3601 gcttccgctt gcttcactaa ttcctgcgta attttcactg ttggtcacta ccacaatcac
     3661 tgtatcatcg tagccagcgt ttctaatggc agcgaaatcg gcgttgatta ataattgtcc
     3721 tgctgacacc tgatctcctg cttttacaaa tacttcaaag ggttcccctt ttaaattcac
     3781 ggtgtcgatc cccacatgga tcaataattc aataccttct ttagaacgta gaccaatcgc
     3841 atgtttagat tctaaaatag taatgacttc tccctcaaca ggtgaataaa tgctgttttc
     3901 ttctggatga atggctacgc cctttcccaa tagtccctta gaaaagacag catcgttcac
     3961 ttcagaaata ggacacacgg ttccttttac cggattttca acaatgactg cttcggctat
     4021 tgccgtttgc tctgattcat cagaagtatc gtcaaatcca attagataag caatgataaa
     4081 ggttgcaaca aaggcgatta cacacgtaat aattgcgtga acaatattca ttggattttc
     4141 tccaataaag gctggtaagg ctgctaagcc aggggaaaca aaggcatatc tgactaaacc
     4201 gctcaagcca gcatacaaac cagcaattgc accaccaatc atggaagcaa ctaatggttt
     4261 tttcagcttc aaggttactc catagagtga aggctcagta attcccatta aagcagtgat
     4321 cccagtggac acagctagct gcttcaactc tttgttttta gttttcgcgg ctacagcaaa
     4381 ggtagcacct gattgagcaa tattagaagc cagcattcct ggcccattga tcatttcaga
     4441 acccgtactg ctcaattgca tagtagcgat tggcgtcatt gcccaagctg tacccgtaac
     4501 caccaaaaat ggctgcaaac cacccatcaa aaatggaatt aaccaagctg ctttgccgtt
     4561 aatccattca gcccccattg ctataagatc gttcagcaac gttccaaacg gtccaatgac
     4621 aaccaacgct aaaggcccca taattaaaaa taccagcatc ggcttagtaa agaatttaat
     4681 aaacgaaggc gaaattcgat ccgcaaaggg ctctacatag gacatgatcc aaacagtaaa
     4741 gataatcggt aaaactgatc ccgcataatc tgcagctaat accggaattc ctagaaaaga
     4801 aacggcatcg ccagtagaaa aaagctgtga aatatttgga tgaagcaaaa tgccagcaac
     4861 agtaatcgcc aaaattgggt tgacatcaaa ctttatggct gccccatagg ctaatagtac
     4921 tggcataaaa taaaaggctg catcagcaat agtattcaaa atgatgtaag tttgactact
     4981 gtctgatagt acaccagtca aagtcaagac ggccaaaatg gcttttacca tcccggcacc
     5041 tgttatagct ggaatcatcg gtgtaaaggt ggtagaaatg acactaatca atcttgaaat
     5101 aatactttct tgattgtctg aagaggggcc ctttttaatt gaagttccca cttccttttg
     5161 aatttccttg aaggctgttt ggacttcatt gccaatcacc acttggtatt gtccaccgct
     5221 atccaccaca ctaattacac caagtgtttt ttctaaggct ttcgtatcgg ctttcctttt
     5281 atctttgagt tcaaaacgca gtctggtggc acaatgggtt aagctgacca cattttcttt
     5341 tcccccaacc tgttgcaata catttcttcc tacttctctg taatccatta ttctctctcc
     5401 ttaaagcagt aaaaaagacc aagtcaaacc acgccccaat caacattgaa gtgttagttt
     5461 gtcttggtct tgcctgcata atcagtaaca tgccaataac gttattttgc tgtaatccaa
     5521 taatagcaca ttatgatagc gcaatcaaca gggaatttaa ttttttatct tttcgtttga
     5581 ccgtataggg taatataaca gttagatcac atcaactagg gtatatctgc gtatacttac
     5641 tgctaaaata agtatatcta atattaacta taccagtaaa accattccta cataaccagc
     5701 gggaaattcc caaaatcgtg aaagcatcaa aaatgcatat aacggatcac acaacttcaa
     5761 caattctgct cttgggaaaa attgatgaat ttggtgagta aaggatggaa taaacagcat
     5821 tccaacaata taggcaacaa tagtgatact tgtttttttg gtaaaaatca gtaagcaata
     5881 gccaatttca atcattccga gatataaagg tatcgataga atgatttggg ctagtattct
     5941 taaaaattaa ct
//
LOCUS       DAZUMK010000142         5890 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000142 DAZUMK010000000
VERSION     DAZUMK010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.145"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..634)
                     /locus_tag="ACG808_002555"
     CDS             complement(38..634)
                     /locus_tag="ACG808_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFS5048146.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            984..1328
                     /locus_tag="ACG808_002556"
     CDS             984..1328
                     /locus_tag="ACG808_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFS5048147.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            1329..1748
                     /locus_tag="ACG808_002557"
     CDS             1329..1748
                     /locus_tag="ACG808_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFS5048148.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1840..2190)
                     /locus_tag="ACG808_002558"
     CDS             complement(1840..2190)
                     /locus_tag="ACG808_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048149.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(2156..3487)
                     /locus_tag="ACG808_002559"
     CDS             complement(2156..3487)
                     /locus_tag="ACG808_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFS5048150.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            3742..4308
                     /locus_tag="ACG808_002560"
     CDS             3742..4308
                     /locus_tag="ACG808_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5048151.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            4453..4540
                     /locus_tag="ACG808_002561"
     tRNA            4453..4540
                     /locus_tag="ACG808_002561"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4485..4487,aa:Ser,seq:cga)
     gene            4876..5850
                     /gene="bsh"
                     /locus_tag="ACG808_002562"
     CDS             4876..5850
                     /gene="bsh"
                     /locus_tag="ACG808_002562"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFS5048152.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNINKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT         2146 a          997 c         1093 g         1654 t
ORIGIN      
        1 acattgtcaa tcaatgacta attttacaaa ataaatatta tccaagaaga ccacttagat
       61 attgacctcc ataaatcaag gccatactat ataagaaaat cgacagtggt ttagcaatca
      121 aaatgattag tgtatatcgt ttcactgaca tgtttgtcaa tccagccatc aaacataacg
      181 catcgtcagg agcaactgga agaaaaatag ctagagcaaa cagcttctca aaacggtttt
      241 gattatccag ccagccaata tatttgttat atgtcttatc gctaatcatg gaaagtatga
      301 atggctttcc ataacgacgc cctagcagaa aaatgataat cgaaccaatg cagattccaa
      361 tatagttata gataaatcca gcataagggc caaatatcag gacaccagct gcagtactga
      421 tccctccagg aatgattgga ataactactt gcaggatttg gatgaagaca aaaataatcg
      481 gtcctaaaat gatggaatca cctactaatc ctcttaacgc attcaaatct ttgaaaacac
      541 ctaaattaat aaaataaatc gtcagcgcga ttgataggcc taatccgata atggatatga
      601 aatttatcag ttttcgagag gcagcaatat tcattattca tcaactcctt agaggttctt
      661 tgttaactat actttactag aaaagcaact aactgcgcca ttttttgata tgacagaaaa
      721 caaacaattt tgtaagttca tcataagtat aaaagattga tctcaaaaaa acatccaact
      781 aaagacttat ttaataactt gtttttttta aaataaaaaa agcgttagca actatgctta
      841 aaaagcatat cgactgaata aactaagcat tggacaggct gaaagactac atttaaaatg
      901 aaatgcgaag aaacaataga aaataaagtt gccttagaac catagttgaa tcacctaaca
      961 gtcgtgaagg gagaataaga gaaatggcag aaaaacaaac cgcaggacga gatcaattag
     1021 gagattttgc accaaaattt gcagaattaa atgatgatgt tttatttgga caagtatggt
     1081 ccagagaaga attatctgct catcagcgta gtatcatcac catatctgca ttaatttcag
     1141 gaggaaattt tgaacaattg ccagctcatc tgaagattgg caagcaaaat ggcattacta
     1201 gtgaagaaat agcagaaatc attacccatt tagcttttta tgtagaatgg ccaaaagctt
     1261 ggtcagcttt tagtttagca aaagaaatct atttagaaga aaatagcgaa gataagaaag
     1321 aggcgtaaat gatgacaaaa cacgaagaaa tacacaatgg cgtgattttt ccagcaggtg
     1381 aaaaaaacga agcttacgct caagtattta ccgggcaaag ctatctgaat atgttagtga
     1441 atgatcccga agtttctgtt ggtgtaggta atgtaacttt tgaaccaggt tgtcgcaatc
     1501 actggcatgt ccatacagac ggctatcaaa tcttacttgt gaccggagga gaaggctggt
     1561 atcaggaaga aggaaagcca gcaagacgat taatggctgg agatgtcgtc gtaacgcaca
     1621 aagggattaa acattggcat ggtgcgacaa aagacagttg gtttgaacat ttggcgatca
     1681 ctgctggtaa ttcagagttt ttagaacctg taacagatga agtgtatgaa ggattggaaa
     1741 atcaataatg tcaagcttga acagctcctg aaagtagatt ttaatcgaac tttcaggagc
     1801 tgcttttttg attcagtctt ttataaatag aaaatagggt taataatagg aatgagaagc
     1861 acgtcgaaag atcttaatat tgataatcgg ctgggcatac atgatttcca cgtcatcaat
     1921 caaactacct aacagatgat aatcttcccg atcatgtcgg ggattaccta atagttcttg
     1981 atagtagatt tccatactag ggtcccgagg attgttcagc aaatcagcaa gtacatctaa
     2041 atcttctggc tgagtttctg ccgtaccctg tatctctatg aaaagccctt cctcagcaaa
     2101 ttctactcca atacgtattt cagttatttc atgggaatat aaataagtaa gaagctcatc
     2161 gatgattctt tttgataatt ctttttccaa gtgtcttttt ttcctttctg acagcggtaa
     2221 agttatctag tatcggtaca aggatacctg caacgaaacc agttgaaaaa ccactgttat
     2281 atagatttag tcctccgtgc aaagtgctga cgttatggac aagagcaata tgtagcacac
     2341 cagctattac accgtaaaaa ctgccgtaaa aaccgcttat gggcgcaaga cctgtcccaa
     2401 acacagctgc aacaagcatg ccagtactgg tgatctcatg aaaagttcca aacaaagaag
     2461 caataaatat tccgactagt atcggaaaac tgttttttaa atgacaacca aaagcactga
     2521 aacccaccgc agataaaata gcaccgatga caggtccatt taactgaccg ccaaccagca
     2581 aaacataaga agacagcatc agaccaacta agcccatgtt gatcaaagta gcaccaatac
     2641 cagaattagc aatgaaatca gttgtcaatt ttccagatga atcgaaaatt tctcgaatcc
     2701 ccgaaagccg aaaggaatta taatagaaac caattccgac tgtcagcgaa aaaaagccaa
     2761 aaataatcca aatgagagga aagtgatatt cattgaaaac caatgtattt tcgacgattg
     2821 aatagctgaa taacctcaaa acagcagtaa aagtcatcgc aatcaaccca gaggtgaatc
     2881 caatattata aagtgtgaat ccacgatgga aaactaaagt ttgagcagct aagggcggaa
     2941 gaagtatacc aattgctatg ccagctaaat tccccaataa aatacctatc agtaaaggaa
     3001 aacgcatacc aaaagtgata taactaacga ccggtgataa tacgctacca aacagtccga
     3061 tcatgatgta ctgagaaaaa ggtttattga caaatacagc gtatagatac acacctaaaa
     3121 tgattgagat agaattataa aggtttttgc caaaaaaaga gaaaccggta actgttagaa
     3181 tcgaagcaaa taagggaccg tttaaaatca ccttgtgccg ataagctaaa agcacagaaa
     3241 gtaaggtcaa aatccctaca tttatgaatg cgcttccgaa aacacctaaa gcaaaataat
     3301 ctgtcaaaag attgcttggc gaggtaagaa tagttagcaa tccactccaa ttatcagtag
     3361 gagtattaga aagcaagcca ataaaaagaa aaaaaacacc tgtaaaacca agataaaaat
     3421 actttctttt ctgtgaaacg attcgttttt gaggatgaat ataaataacg tcagtttcgt
     3481 tttccattac acaccttctt tttcattttc tttaactata acttgaaatg aaagatttgg
     3541 acaactatca tttaggtcaa aactaaaaat gtaagggaac ccattgaatt taaccaaatt
     3601 tttttaattt tgcaaaaaaa agttaaagaa gactgaaaaa aatttaaaat ataacgtttt
     3661 gagtaaaatg tttgcaaaaa atgctaaaaa tagggtacaa taaaacaaca tatactgcag
     3721 taaagaagtg ggggagtttt aatgagaaga aaagtctata ccaaggatca aattctaaaa
     3781 gctgcttatg atgttgttgc aaaggaaggg tttaaaggat ttactgcgag aaatattgct
     3841 aaaaagatgg ggatttcgac acagccgatt tatctggaat tcaaaaacat ggaagattta
     3901 aaaaatacgt tgctggattc tatctttgaa caactaaaag aagatgtttt ccctattgaa
     3961 cgtactggtg ataaaatcat tgatctcggt ttgaactata tctactttgc aaaaaagcat
     4021 catagtcttt acatggcatt gtacattgat gaacatgggg acggaaagaa aatgcatgat
     4081 ttttcataca attatttccg tgaaatagtt aaagaagatg aaagatacaa tcaattatcg
     4141 gatgaatatg tcgatgctct gcatactggt acatgggtag cagtgactgg cttagcgtca
     4201 ttgatgtcat caaacatcat gcatccaaca gaagaacaaa tcattgcact gatcaaacgg
     4261 acaattgact cgattttagc attagataat cctgaaataa tcagatagtg ttctatcagt
     4321 atgaatttga tcggtaagtc cgttggctgc aaactgtcaa tggacttttt tgctaaaaaa
     4381 agcgatttct gttatttatc tattagaaat aggcttgaca tttcgtgaaa aaagtttaaa
     4441 aatagtactt acggaaggtt ggcagagtgg taatgcaccg gactcgaaat ccggcgaacc
     4501 ggctaatacc ggcgcgcagg ttcaaatcct gtaccttcct tatatcaacg attagaggtt
     4561 gtgaagaagc tgtcctgcat caagtaataa gaacggccaa acaaaaatag cttttcctat
     4621 tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttgttcgga
     4681 ataaggggct gtgacaagac ttttgtcaca gccccttatt gttgtacttt taacctacaa
     4741 tattttttga ttccccctga gtactatttt ttataaatgc tagtaaatca atcttctttg
     4801 gccttttttt tgataaaaaa aaatactaga tgtaagataa aagtgtaaaa acgatttgtt
     4861 ttaggaggga aaacgatgtg taccgcaatc acttatgcaa cgaaagacca ttattttgga
     4921 aggaattttg attatgagat gtcttataat gaagtagtca ctattactcc aagaaattat
     4981 cgtttcgatt ttagaaaggt aaaaaattta gataaacatt atgcaatgat aggaatagca
     5041 gctggggtat ccaactatcc actctattat gaggctacaa atgaaaaagg gttgagtatg
     5101 gcaggactga attttcctgg caatgctgat tataaagaat tacaagaagg aaaagataac
     5161 gttgcaccat ttgagtttat tccatggata ctagggcaat gctcaaccat agacgaagct
     5221 aaagaattat tggctactat caatctagtc aatatcgatt ttagtgaaaa actacccttg
     5281 tcacctttac actggttatt agctgataaa gaaaaatcga tagtaattga aagtatgaaa
     5341 gatggacttc atttatatga taatcctgtc ggtgtgttga caaataatcc gccatttgat
     5401 tatcaattat ttaatctgaa caattatcgc tcgctatcaa atggaacacc agaaaatcat
     5461 ttttcaaacc agatcagctt gaatgtttat agtcgtggga tgggaggatt gggcttacct
     5521 ggagatctct cttctgtttc tcgatttgta aaagccacat ttactaaaat gaatgctgca
     5581 tcaggtgatt cagaatctga aagtatcagt caattttttc atatactagg ctcagtcgag
     5641 caacaaaaag gagtatgcga tactggagaa ggaaaatacg aatatacgat ttattcttct
     5701 tgctgtaacg ttgataaagg gatttattat tatcgtacgt atgaagacag tcaaattact
     5761 gctgtaaata taaataaaga agacttggat cgtcatgaat tggtcagtta tccagtcata
     5821 aaagagcaac agataaacta tgtcaattaa ttaatgaata gatccgtttg gtatcatatt
     5881 ttctgataca
//
LOCUS       DAZUMK010000143         5877 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000143 DAZUMK010000000
VERSION     DAZUMK010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.146"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            641..1375
                     /locus_tag="ACG808_002563"
     CDS             641..1375
                     /locus_tag="ACG808_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5048153.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1391..2560
                     /locus_tag="ACG808_002564"
     CDS             1391..2560
                     /locus_tag="ACG808_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS5048154.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2576..3571
                     /gene="lacD"
                     /locus_tag="ACG808_002565"
     CDS             2576..3571
                     /gene="lacD"
                     /locus_tag="ACG808_002565"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFS5048155.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3650..4576
                     /locus_tag="ACG808_002566"
     CDS             3650..4576
                     /locus_tag="ACG808_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFS5048156.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4595..5845
                     /locus_tag="ACG808_002567"
     CDS             4595..5845
                     /locus_tag="ACG808_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS5048157.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         1967 a          955 c         1204 g         1751 t
ORIGIN      
        1 gtttgaaaaa tgctatttcg aggtagaagg atattatata agcacggttg actaaacgaa
       61 gtgataataa aaaaagttat tcaagaataa gaaaaattat gatacgaatt cagataaact
      121 ggagaataca gagaccttta aaagggataa gaaacaacat aaattcctct taagagagaa
      181 cgaaaggaca aagccagcaa cttgatacac gagctagtat tagaggaatt tattctcata
      241 gactcccttt tgaatacgtt tcaactttaa ttttcatgtt cataaatgat ttgaaaccaa
      301 agcaaatgcg ttgcactttt cctccgtctt ccgaaaatcg ttcaattcac tggtaagtag
      361 tcatttgata gtttgaggca aattgttgat tgatgaaggg taggagctaa aaaaagatac
      421 cagttgaaga gagaaaaaga aaaaaataga gggtgttctt gaatgaaaaa agttggaaaa
      481 ttatttttca tttcctttta ttcctattaa agtcagtgat gtcaatcata ttaacaagac
      541 taaaaatgaa aataaaaaaa agattgttct tagtattgac tcataaaaaa taccactact
      601 atacttacat tgtggttata gccagttagg agagacatcg atgcaaaata cctttcaatc
      661 aaaatcactt tattatcagc tggtagaaat attgaaacaa cgaatcgaaa acagtatggt
      721 cgctcatgac aagctgccat cagaaagaga gttgaccagt ttttatggcg ttagtcgcac
      781 gacggtgcga ctagcattgc aagaactgga aaaaatgggc tacatttatc gacaacatgg
      841 aaaaggaacc tttgtttcgg aattaaggac aaatgcgact gatctagctg gcgcatatag
      901 ttttaccgaa caaatgcggg caatgggcaa acatcctgaa accaagatat tggccttcaa
      961 gaagattgaa gcaactaaat atttagcgag tcatctgaat gttcaactag gtgagccttt
     1021 atttcaatta gaccgattgc gcttagctga tgatttgcca atgatgatcg agaaatccta
     1081 tttgccagtg aagctattta tgtccctttc tcaagaaatt ctatttctga aacctttata
     1141 taatttgttt tcagagaatt tcaatcaaat cgtacggttg gctgacgagg aattttatgc
     1201 cagcattgtc cagaaagaag atagtgagtt gctaaatatt ccagaaggat caccagtgct
     1261 ccatttagtt cgctcaacct ataatctcga gaacgaagtg attgaattta cattaagtgt
     1321 agcgcgggct gataaatttc attataagat caggcatctg cgttccacta actgaattgg
     1381 aggaaagaaa atgtttatgt tctcaaaaga agaactacga aagctaggag cagaaaatac
     1441 tacccgagaa attcgacagc aaccggaact gtggcatgag acgtttgacc gttatcaaga
     1501 aacagttgca gatttagatc gtttgtttac ggatgcgaaa caacatgctg gagagcgacc
     1561 aatccgagtc atttttacag gggccggaac ctcgcaatat gtgggagata cactaattcc
     1621 gtttcttact agtcaaggaa atacgaacca gtttattttt caaagtattg gttcgaccga
     1681 tatcgttgct acgccaaaag aatacctgat tcctgatgag acaacgatct tggtctcatt
     1741 tgcccgtagt ggaaatagtc cagaaagtct agccgcagtt aagatggcca atcagctggt
     1801 caacaagatc tatcatatcg tgatcacctg tgcaccagac ggattattgg ctacacaaat
     1861 gcctacaaaa aaagaaaatc tagttttact aatgccaaac aaagccaatg acgctggttt
     1921 tgctatgaca agcagtttca gctgtatgac gctgactgcg ctacttattt ttgatcaagc
     1981 aagcaatcag caaaaagaaa gatatgttca tcaattaagc cgactaggag aagaagtcat
     2041 ccaacgagaa tcagagatcc aagggtatct ggcatcagac tttcatcgca tcatttactt
     2101 aggttcggga agcttaggcg gattgactag agaagcgcaa ttaaaagttc tcgagttaac
     2161 tgctggaaaa atcgcaaccg tatttgatac atctttagga ttcagacatg gtcccaaatc
     2221 ttttgtcaat gagcaaacat tggttttcct gtttgtcagc aatgactcat atacgcagaa
     2281 atacgatgta gacatgctgg aagaaatcaa aaatgacggt attgcccaac gcgttgttgg
     2341 aattggtcaa aagggttcat tgtttagtgg tgattccttt gaattagttg cagattcgtt
     2401 actgccggat ggctatcttg ctttagcaga tatcatggta gcccaaacga tcgcattact
     2461 ttgttcggtg aaggttggta atacaccaga tacaccatcg ccaagtggaa cagtcaatcg
     2521 tgtggtcaaa ggcgtaacga tttatccata tgaaaaatag gaaagagtgt gaagcatgtt
     2581 agaatggtcg gaaaataaaa gacaagcaat ggaacgtcta tcgacgccag aaggcattat
     2641 taccgctttg gcgatcgatc agcgaggcgc gctgaagaaa atgatgtcgg ctttagggat
     2701 ggaagcgaca aaggaaaaaa ttgagcagtt taaaacacta atttcccaag aattgactcc
     2761 ttatgcctca gcaattttat tggatcctga atacggactg cctgctgcac agaagcgtgc
     2821 agcagcgagc ggtctgctgt tggcatatga aaaaactggt tatgatcaaa ccgtacccgg
     2881 ccgcttgccg gatctgctgt cggactgttc agttctgcga ttgaaagagt taggtgcgga
     2941 tgcggtgaaa tttttactgt attacgatta tgaagaagca gaggaaatca atcggcaaaa
     3001 gcaagttttt gttgaacgaa ttggaagcga atgcatggct gaaggactgc cattttattt
     3061 agagttattg tcatatgatg ctaaaaatgt agatacgaaa tcgctttcct atgccaaagc
     3121 aaaaccgcgc aaagtgatcg aaatgatgaa agaattctcg aaaacccagt ataaagtaga
     3181 tgtcttaaag gttgaagtac cagtaaacat gtcatttgtt gaaggaaatg caacagggga
     3241 gatcgcctat acacgtcaag aagctgccgc ttattttaaa gaacaaagcg atgcaacaga
     3301 tttgccgtat atcttcttaa gtgctggggt atcagcggca ttattcatgg agacattgcg
     3361 ttttgccaaa catgctgggg cacgattcaa cggtgtactc tgtggcagag caacttggaa
     3421 agatgcagtc gctcctttcg ttgaaaaagg agaagcggca actttagaat ggctgacaga
     3481 acaagggaca caaaacattc gtcaattgaa tgaagtgata agagaaactg ccatcccatg
     3541 gtatgagaaa gtaagtgaaa gcacttgtta atgtttctaa tatattagta gtgataagag
     3601 atatgtgtga ttataattta tcattattga aactgggagg atattttata tgaataaaag
     3661 attgttagat tattcagctt cagatttttt gaaagctaag ccgatggaat taaagcaggc
     3721 aattttggct tctgaaggta gaacaattat ggcagaaaat gtgccaagta gctgtcctta
     3781 catgggaaaa tcagtgacaa atgcagaatt ggagagagct gctggagctg atttaatttt
     3841 gtttaatgca ctagatcttt ttaatcctca aatttcaggt gtacctttaa ctctaaaaga
     3901 gaatccaata atctggatta aaaaagcttt aggaagagct attggtgtaa atttagaacc
     3961 tgtagacaaa gaagttttga tgagtgaaaa tcgaactgaa atttcaagtg gtagaatcgt
     4021 ttctcctgag actttaaaaa aggcaaatga attaggcttt aattttattt gtttaactgg
     4081 aaatccagga acaggagtat ctaatagaac tattgagaaa gcaataacat tatccaaatc
     4141 aatttttaat ggattaatta tcgctggaaa aatgcatggt gctggtgttg atgaaccaat
     4201 tatgaactta gatatagcaa aaagatttat taaaacaggt gtagatattt tattagttcc
     4261 tgctccatac actgttccgt atttcatgga aagtgatttg cgaggaatcg ttgattatat
     4321 aaaagatttt aatagaaata aatctattga ggaaaaagtt ctagtactaa cggctaatgg
     4381 aacaagccaa gactcttctg accaagaaac tattaaaaaa attgcattag catcaaaagc
     4441 atgtggagct gatattcaac acatagggga ttctttaaat ggtatttgtc ttccagaaaa
     4501 tatttttgct ttgggtcaag caattcgggg atatcgtcat caaattatta tgttaggaaa
     4561 atctaattat cgttaattaa ggaggaaatg aaagatgatc gacaaagttc aaaaaatagc
     4621 tgcaaaaatt agtaatcaaa gacatctact agctttaaga gacagcatgg ctgcagttat
     4681 gccattaatc attattggtt ccttttttat gttattagca aatttcccga tagaaggatt
     4741 tacaaaatgg ttatctgaaa ttggagtaca atcctttttg aacaaagcat cagattcaac
     4801 atttggtatt attggattag ctgttaccta ctctttggga cacaatctag caaagactta
     4861 tggtgtagac tcattatcag caggattatt atctttgagt ggatatgttt tacttacacc
     4921 tttattggat gcagatggtt ctataggatt tccaacaaaa tactttggta cctcgggtct
     4981 ttttgttggt attattgtag cacttgtatc tacagaaatt ttccggtggt ttgtacttaa
     5041 aaatattaca attaaaatgc cggcaagcgt tccaccaaat gtaagccgag ctttcgttgc
     5101 tattattcct ggcttttttg tcgttctact atggtttatt gttgttgtaa tttgttataa
     5161 acttggaatt gaaaatgttc atgctcttat tgcagataca ctcgcaaaac cactatcttt
     5221 actgacgaaa actttaccag gaattatatt ggttatactt attcaatgtt tcttttggat
     5281 gtttggtatt catggtgcac aggtaacggg gccaataatt gaaccgttat tattgcaaaa
     5341 ctctgatgta aatagaattg cttatcaagc tggaaaagaa cttcctaaca ttataactta
     5401 tgaattctta tataactttg ttttttctgg tggtgcaggt tgtttatttg cacttgccat
     5461 tttaacattt ttcttttcaa agagtcagga aaataaaact ttaggtaagt tatcaatcgc
     5521 tccagtaagt tttcaagtag cagagcccct tctatttggg tttccaacta tattaaatat
     5581 aaaaatgtta attccatttg taactgcacc agtagtaaca acattaatta cttatttttc
     5641 aatgagtatg ggattagttg caaaacctgt aggagcaaca atcccatgga caactccacc
     5701 gataattgcc ggatttttag cttctggtgg acgaataagt ggtgcagtga ttcaagtaat
     5761 aacaattgca ataaatgttt tgatatacta tccattcttt aaattagatg ataatgccaa
     5821 gctaaaaagc gaaaaaaatg attagaacta cctatatgtt tctaactgta gttaagt
//
LOCUS       DAZUMK010000144         5769 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000144 DAZUMK010000000
VERSION     DAZUMK010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.147"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..450)
                     /locus_tag="ACG808_002568"
     CDS             complement(<1..450)
                     /locus_tag="ACG808_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5048158.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEAFLIAAQQLEKMGYVNENYLN
                     GLIAREREFPTGLITKYLNIALPHSECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVE
                     NLFFLGIKEPKEQVGLLQAFMNLFMDETFVENYKKQETPQNIYELITN"
     gene            674..1465
                     /locus_tag="ACG808_002569"
     CDS             674..1465
                     /locus_tag="ACG808_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS5048159.1"
                     /translation="MLKKERQEQIITLLEKDFYLTNSEIANKLGVSEMTIRRDVTELA
                     KKNKVKKLYGGVERKEPYNRELSTQEKIDTNVAEKKYIGQVMNSMIPDNSTIYVGAGT
                     TILYALPFIQKKNLFVITNSLIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSF
                     ESLNVDISFAATNGIFDNNVTTAQFIEGNIQNAALNAAKIKCIVADYSKLNKSDVYTF
                     RKLTDFDYLITDNHLSEQDFNHYSKYLTILKEPKNDTDNHDEPIH"
     gene            1431..2384
                     /locus_tag="ACG808_002570"
     CDS             1431..2384
                     /locus_tag="ACG808_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HFS5048160.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
     gene            complement(2782..3756)
                     /locus_tag="ACG808_002571"
     CDS             complement(2782..3756)
                     /locus_tag="ACG808_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5048161.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YHAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
     gene            3871..4305
                     /locus_tag="ACG808_002572"
     CDS             3871..4305
                     /locus_tag="ACG808_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS5048162.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            complement(4430..5452)
                     /locus_tag="ACG808_002573"
     CDS             complement(4430..5452)
                     /locus_tag="ACG808_002573"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFS5048163.1"
                     /translation="MKKIGFLSFGHWMEQGSLVKSAQDAYLQSIDLAVEAEKIGVDGA
                     FFRVHHFAPQIGSPFPLLAAIGAKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQG
                     RVQLGVGRGSPEQVLDGWKYFGYDYSEEEIKEVTRERTLEFLELLEGKPFEEPNPQPM
                     FPQGSGKLRIEPYSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQ
                     QANQLRAYKEAWKQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYH
                     RNPTIFGKTFAGEPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIA
                     PELGWR"
BASE COUNT         1809 a         1088 c          989 g         1883 t
ORIGIN      
        1 atttgtaata agttcgtata tgttttgcgg tgtttcttgt ttcttgtaat tttccacgaa
       61 tgtttcatcc atgaataaat tcataaacgc ttgaagtaat cctacttgtt cttttggttc
      121 tttgattcct aaaaaaaata gattttctac agtcatttcc tgattatccc ccatttgata
      181 aaaataaatt gggttcttca gctgcataac aaaaacaaat ggcttttcga catattcaca
      241 ttccgaatgt ggcagcgcga tatttaaata cttagtaatc aatccagtag gaaattctct
      301 ttctcttgca atcagtccat tcaaataatt ctcattgacg taccccattt tttctaattg
      361 ttgtgcagcg attaaaaaag cttcttcttt attttttact tctaccgcta attgaatcaa
      421 actaggaacg aacatctctt tatatttcat tccaacctcc tcctttttga ttaagtttgt
      481 taatttattg ttctactaag ttcatctttg ttttacaaga acattatatt tctgttagtt
      541 tgttttgtca acgctttcaa aagaaattta ttttgttaaa tttgatctac ttgaaaataa
      601 aatcgtttat ctatataatt aagaagaaca aagtcgaaca aaaattgact tttatgttaa
      661 aggagtaatt catatgctga aaaaagaaag acaagaacaa atcattacat tattagagaa
      721 agatttttat ttaactaatt cagaaatcgc taacaaatta ggcgtatcag aaatgaccat
      781 acgccgagat gtcactgaat tagcaaaaaa aaataaagta aaaaagctat atggtggcgt
      841 tgaacgaaag gaaccttata atcgagaact atcgactcaa gaaaagattg atacgaatgt
      901 agccgaaaaa aaatatattg gtcaagtcat gaacagtatg attcccgata attctactat
      961 ttatgtcggt gctggaacaa cgatcctata tgcacttcct ttcattcaaa agaaaaatct
     1021 atttgtaatt acaaacagtt tgattgcttt caactattta aagaatcata caagttatcg
     1081 gattttactt actggtggcg aattttctcc tattacagaa gagttcattg gggaagttgc
     1141 agaaagatct tttgaaagtt tgaatgtcga tatctctttc gctgcaacaa acggcatatt
     1201 tgataacaat gtgacaaccg ctcaatttat tgagggaaat attcagaacg cagcgctcaa
     1261 tgcagcaaaa atcaaatgca ttgttgcaga ttattcaaaa ttaaataaat cagacgtcta
     1321 tacttttaga aaattaaccg attttgatta tctaataacc gataatcatt tatcagaaca
     1381 ggatttcaat cattattcaa aatacttaac aatattaaag gagcccaaaa atgatactga
     1441 caatcacgat gaacccatcc attgattcca tttattttac aaaacacttt gaattaggta
     1501 cgatgacacg ctttgattct ccggtacgct atgtaggcgg caagggaatc aattgcggac
     1561 gagcttctgc tctactaggt tcatcagtta ttttaacagg gtttttgggc ggttattttg
     1621 gcagtatgat tcaagatatg ctgaaaaatg aaaatttgtt tgatttgcat tttttaccta
     1681 ttgcagaaga atcaagaggt gccgtcacga tcatgcatga tgatgaaacg cagacagagc
     1741 tagtggaagc tggtcctgaa atagcagata ctactatcaa aaaattaatg aatgatatca
     1801 aactaatcag taaaaataat tacctttcag tcattacaat aaatggttct gttaacaatc
     1861 cagatccctt tttgtatgct aaattattac aatatatcaa agaacaaata gatccttcca
     1921 cctatatatt aatggatata tctggacaac atctccgtga agtcttagca agtaagtata
     1981 aaccgatgtt catcaaacct aatatccatg agttttcaga tatcatccat caaaaaatta
     2041 ctcaaaaaag cgacataaaa caccatctaa aaacaaatcc actatttaaa gatatcgatg
     2101 ttatcatgat ttcttgcggc tcgtatggtg cagtagtcaa agtaaaagaa gaattttgga
     2161 atgttgagat ccctaagatt cctatcatta atacaacagg ctctggggat gccactgttg
     2221 gaggttttgc tcattcatta gaacagaatt atcctttaaa agaatgtgta aggtacgcaa
     2281 tggcttgcgg catgtcgaat gcacagcaat cagaagtcgg gaaagttatt ccagctgatg
     2341 taaaaaattt agcaaaaagc attaaaatca cagaaatgaa ataaatagta caaagtaaat
     2401 aattgtttat atgaaatgag acacaaagag actgtgacaa aaaacgtgtg taccgacccc
     2461 caaaagttag attttttggt ctaacttttg ggagtcgata cattgtcaca gtcctttttt
     2521 cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
     2581 gagaagctat tttttgaaat tgctccttgt gactcagagt taaacggcag gctcacgacc
     2641 tcttggtata aacggtgttc aaaagcttgc atctacgcta atgagtccaa ccaagtcaaa
     2701 aatgaggagc ttttttggct gtccttatta cttgatgcag ggcagttttt tcataatctc
     2761 ttttagatta aatccttttg gttacacaag gttctcttca aagaatgttg ccattttttc
     2821 aaatggaatg atgtctgtct ggtcgtatag atctgtatgg gatgctcctg gaatgatcat
     2881 gagttccttg ttgtcacctt ttagttcttt gaaagcgtcc tccccaaagt aacgtgaatg
     2941 agctttttcc ccgtgaatga ctaagactgc atgacggatt tctggaatat aatgcaatag
     3001 ttttgcattc ataagtgaca accctgatgt cctattccaa ccatcattgg agtttaaaga
     3061 acggacatga tatccgcggt ctgttttata atacgcatga tagtctttga caaattgagg
     3121 agcatcttca ggcaacggat caaccacacc tccagcacgt gcgtagtccc cttgtttgaa
     3181 atcaatgatg cgttgtgcat ttaattcttc acgcatttca tatcgggcat tttcatccat
     3241 aacatcgaag tagccatttg ctgttactcg agtcatgtca tacattgtgg aaacaactgt
     3301 tgctttgacc cgcgtatcga tagctgctgc attcaatgcc atacctcccc atccacaaat
     3361 accaatgatt ccgattctct cagggtttac atttttttgt acagataaaa agtctactgc
     3421 cgcttggaaa tcttccgtat tgatatcagg cgaagcaaca tatcgaggta ctcctccgct
     3481 ttctcctgta aacgatggat caaacgcaat cgttaaaaag cctctttcag ccatcgtctg
     3541 cgcatataga ccagacgatt gttccttcac tgcaccaaat ggcccgctca cagcgattgc
     3601 cggcaatttc ttttcactta cttcttttgg ttcatataaa tcagcgacta atgtgatacc
     3661 aaaacgattc gggaagctca cctttcgatg attgacttga ttgctttttg gaaatacttt
     3721 gtcccattct tgtgtgatgt ttaattcttt attcatattc tagactctcc tttgatcaaa
     3781 attgacattt gtttttgaat gtattattat gataaaactt aaacttgact ttaggtcaag
     3841 gtaaaaattt tattttagga ggattttttt atgtattcca ttggacaagt gtctgaaatg
     3901 tttggattgc ctgtttctac tttacgttat tacgacaaag agggattatt tcctgatatc
     3961 cagcgtgttt ctggtattag aaaattttca gagaatgagt tagaggcttt aagagtcatt
     4021 gactgtttga aaaaatctgg tttagaaata aaagacatta agcaattcat ggaatggagc
     4081 aaagaaggca gtaagacatt tgaggttcgt aaagaactat ttgaaaaaca aaaagaagta
     4141 gtagaaaaag aaatagcaaa attagaaaga gtactcgcta tgctaaacta caaaagctgg
     4201 tactatgaag aagcaataaa agccggcaat gaagaagccg tattgacaat gattcctgat
     4261 gatttacccc aacatgtaaa agaagcttat gtacattctc attgaacaaa cggataactc
     4321 actctccttc tctatgaact tcatgaaaac atgaataaac acctctaact gattgttaga
     4381 agtgtttatt ttttcactca agtatcctgc acttacaaaa atatcctttt taccgccatc
     4441 ctaattctgg agcgatatct tttacgattg tttcaagcaa gtgcatgtta tattctaccc
     4501 ccaaagtatt aggaatcgtg acaagtactg tgtctgcttc tttgattgct tcatcttcag
     4561 ctagctcctt gattaattga tcaggttcac cagcaaaagt ttttccaaaa atcgttgggt
     4621 ttcggtgata agcaagatag ccgacagcat cttgttgcgg actaccgtct tgtccaaata
     4681 agcgtcgatc caaatcagtg gtgattggct gaatcgagcg agtaactaat gttcttggtt
     4741 caaaatcatg acctgcttgc ttccacgctt ctttatacgc acgtaattgg tttgcttgtt
     4801 ggatatggaa tggctcgtgg gattcgtaat tgactaatgt agatgattgc agattcattc
     4861 catgttgtgc tgcccagatt gccgtttgct gcgaaccagc tccccaccag attctttgtc
     4921 gtaatccttc tgaataaggt tctatccgta attttcctga tccttgtggg aacattggtt
     4981 gaggatttgg ttcttcaaat ggttttcctt caagtaattc caagaattca agggtgcgtt
     5041 cccgtgttac ctctttgatt tcttcttcac tgtaatcata accaaagtat ttccagccat
     5101 ctagaacttg ttcaggtgaa ccacgcccca ctcctagttg cactcttcct tgcgtgatga
     5161 tatctgtcaa tccggcattt tctgccatat aataagggtt ttcatatcgc atatcaataa
     5221 gtcctgtacc gatttcaatc ttacttgtct ttgcgccgat tgccgctagc aaaggaaacg
     5281 gcgacccaat ctgaggggca aaatgatgta cgcggaaaaa cgctccgtcc actccgattt
     5341 tttctgcttc caccgctaaa tcaatcgatt gcaaataagc atcttgagct gatttcacaa
     5401 gcgatccttg ctccatccaa tgtccaaagc ttaaaaagcc gatttttttc aaggtaatga
     5461 cctcctttaa acctacaaaa atggaaggtt ttttattttt aatatgaact gattctacta
     5521 ctttttttct taaaagacga ataattgttc tcgcctcaca atcaagaaat gtatcggctt
     5581 actacacccc tactttcttc atcatctgcc gtgtcttaat ctcttttaac agtctgttac
     5641 atcgttgatt tttgactcaa aatgattgtt tctaacatgt atttatggta aaaaagacaa
     5701 gattagttct ttgtacacat cgatttatac gctgaacaaa aagaaatata taaactaaga
     5761 aacccattg
//
LOCUS       DAZUMK010000145         5762 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000145 DAZUMK010000000
VERSION     DAZUMK010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.148"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1019)
                     /locus_tag="ACG808_002574"
     CDS             complement(<1..1019)
                     /locus_tag="ACG808_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HFS5048164.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFA"
     gene            complement(1033..2250)
                     /locus_tag="ACG808_002575"
     CDS             complement(1033..2250)
                     /locus_tag="ACG808_002575"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFS5048165.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(2240..3835)
                     /locus_tag="ACG808_002576"
     CDS             complement(2240..3835)
                     /locus_tag="ACG808_002576"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFS5048166.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYSISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLSSTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            3961..4746
                     /locus_tag="ACG808_002577"
     CDS             3961..4746
                     /locus_tag="ACG808_002577"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HFS5048167.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVVNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKPTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
     gene            4739..4981
                     /locus_tag="ACG808_002578"
     CDS             4739..4981
                     /locus_tag="ACG808_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048168.1"
                     /translation="MTNNILISIYPRYMEKIINKQKNFEFRNFYLTSDPYNIFWIYET
                     IPTKALKYKLIVKNPITKLKKNQHYFLGDDEFEQLV"
     gene            5218..5616
                     /locus_tag="ACG808_002579"
                     /pseudo
     CDS             5218..5616
                     /locus_tag="ACG808_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5628..>5762)
                     /locus_tag="ACG808_002580"
     CDS             complement(5628..>5762)
                     /locus_tag="ACG808_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS5048169.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
BASE COUNT         1794 a         1126 c          966 g         1876 t
ORIGIN      
        1 gcaaattttg taaactctgt ggtggtttga ttatctaaat ctttgcggtc cactaacatg
       61 atcgttcgat cgatccccga ttttcgtgct aaaagcttag tagatacaaa actagtcaac
      121 gtctttcctg aaccagtcgc atgccacaca taccctgatt gatgtttatt tgcggcaata
      181 aacaaggctt gaatcgcatg cacttgataa ggatgtaaca ccatgagggt tttattgtct
      241 tgatcttcac tgacaatcgt ataatccgca atcaagcgat gggcatctgg gatatttagc
      301 acttgtttgc aaaactcata taagttttct acctttttat tatctttcgt tcgccaacta
      361 aataaaaact taggatgcaa atccttcggt aaggcatttg caaaataccg agtcgtttgt
      421 tcattggaaa ccacaaacaa ctgtaaggtc gaaaagatat tgttccgaaa catgccttct
      481 tcagcatatt ttttgatttg attaaacgcc tgataaaaac catcttttgc agttacttgt
      541 tttagctcga tttggataat cggtaagcca ttgatcaata aggtgacgtc aaaacgtcga
      601 tcccgcgctt ctatgttact tccacgtttt gcaatttgat ggaccacttc ataagtagag
      661 attcctccac caatgtcttg attggaataa aggataagtg agacagaacc caattgcgga
      721 ttttcccgtt caatcgtgat ccgagccatc ccattttcac cttttaacca tttcgcagca
      781 tcaaatgggg ttttcgtccg tagcaataat tcggttttga tcgtttcaaa ctctttatcg
      841 gtcaaaggag aatcattcag ttctgcttga ttatttgaga tgattttttg acgtaaattg
      901 ctccaaagat cttcttctga ttttagatcc ggacgatacg tccactgatt atgtccttcc
      961 ccgaggacac gaatcaactg ttcttcgact tccgcttcct cacggtgact aattttcatt
     1021 cttcttccct ccctaaacaa acatcttttg caagaagcct tttttcgttt ccttcagcaa
     1081 atctaactta cgttgatgaa gagcgatcgt atcatctagc tgcttgaaga aggcaccaat
     1141 tttttgttgt tcatttatat ccggtggaaa ctgaaatgtc ctttgttcta taccaccttt
     1201 agtcacatgt accattgtcg ttccgttagt tgattcttta attttctctt tatcagtcaa
     1261 tagccaagta tatagatact gtttgtccaa ttccttagat aaaaatttaa gtttccaaat
     1321 atgataatgg tagattacac gttcttcatt ccacatttca ggaccgaagc ttgttgccca
     1381 taaatataag agatctcctt tattagcata cttgtcttcc tctaattcta agtttgaata
     1441 ataccatcta tcattagtat taaaattacc tactcttaaa accctatatt ttccagaatc
     1501 aagtaattct tgttgtttaa aagctctacc attcaaaaaa actaccactt cccccaactt
     1561 ccgctcttcc caatctcccg taaatcctgg aaaacgaact tctggcactt ttgctccgtt
     1621 tttagggaac attttttgta agaatccttt tttcgtttcc ttcagcaaat ctaattcgtg
     1681 ctggtgaagc atgatggtgt gatcaagttg tttgaaaaat gaaccgatct tgatctgttc
     1741 gtcataagtt ggaagtgaga taggtgtctt tttaccacta tcaattgtat aggttccgac
     1801 ggttccaatg cctgcaacgt cacggatata cttctgaatt ttgttattta tgaacgagta
     1861 gtaaaagaag tttccgtcaa ttttatgctc attcttgaac caaatgatgt ttccatcctt
     1921 aaagtaaatt gggttatcat cagtgattaa taaggggaca cctattgatc cgacaccagt
     1981 tacaagcaag tctccttgag aaactttacc tgaatttttc gaatattcat tatatttttc
     2041 ttctgaaatg taaagataat cggatggttc ctcatttttt gcagctgaga caatatcacg
     2101 agcacgtaaa aatctaatac ctgagttagt ccaatctgat tgatgaattc gcttcacaga
     2161 agtaacatcc atcatatctc ctaacttacg ctcttcccaa tcttccgtaa aaccaggaaa
     2221 ccggatttct ggtcgtgtat cattgctcat gtttgaacac ctccagcgca ccttgtaacc
     2281 acgcttcatt ttcaggtgtt gtttgcaggg aagagatagc ttctaaaagt tctttttcta
     2341 gcacttgctg ttcttgtcgt atcttcttga tgtcatttcc gacatgaacc atatcaattg
     2401 gttcttcttc ttcgaaagta tctacatagc gtgggatatt taagttatag tcattttctt
     2461 tgatttcatc aaaggttgcc aaatgagcgt atttctcaac atcttttctt tcagcgtagg
     2521 tttcgaggat tttttgaatg ttttcttccg atagtttatt ttgatttttc cctttgacaa
     2581 attcacgact agcatcaatg aataatacat ctcgagtttg acgattcttc ttcaatacaa
     2641 tcaccgtcgt tggaatcgat gtcccaaaga acaagttcgc aggcataccg atcacagcat
     2701 aaatactgcc gtcttctaat aatttttgac gaatgacccc ttctgcagcg ccacggaata
     2761 aaactccgtg tggcaagacg atagccatgg tacccgtttc cttcaaatga tagaaaccat
     2821 gaagaagaaa cgcaaaatct gctttggatt ttggcgctaa ttttccatag cgattaaacc
     2881 gagaatcatc taagaaggtc gtatctgctg accaattagc cgaataaggt gggttcatca
     2941 ctactgcatc aaatgtgtaa ggctcatctg ttggccagtc cttattcaac gtatccccat
     3001 tgcggagatt catttcttcg gcgtcgacac catgtaaaat caagttcatt tttgctagat
     3061 tgtatgtcgt tgtatttaat tcttgcccat gatatttaac gttatctggg tgagttaagt
     3121 agttacgtac gtttaacatc aatgaacctg aacccatcgt tggatcaaaa acactaaaga
     3181 atcgtcgttc tttttgatcc aacgtcacga tttgtgccat catatcagaa accatatgag
     3241 gcgtgtaaaa ttctccggct ttcttccctg cttctgaagc aaattgactg atcaaaaatt
     3301 catacgcatc cccgatcacg tccccatcgt gacctaaaac ttccacgtca ttcaatttct
     3361 taatgacttc ggtaatcgtc acgtttcgtt gttgctcatc tgttcctagt ttttttgatt
     3421 ggagatccac atcatcaaat aacccattaa attggttata agtagaagac aattgcacaa
     3481 aggctttatt taaatcattc aattggaacg ttgcttgttt tgcttgatcc gctaagacgt
     3541 tgaaaagata ctctggtgag atcgaatacc ccaaaatatc aacgattgtg gcaatcaggt
     3601 cttcttttga ttcttcatct gaaagtaatt cacgatacaa cattgtttgt ttgcttactg
     3661 tgtcatattc ctctaaggat tcatctgcta aaagaaccac ttgttccaac aaacggtcgg
     3721 ataaatattt atagaaaatc agtcccaata aataattttt atattctgac gcatccattt
     3781 tactacggag attatctgcc gcactaaata atttttgatt taattctgca cccaaaaaga
     3841 tacactccta tctcttattt aaagttgcta tataccctct tattttagca tactcccttt
     3901 tagaaaaagc aaaaaaaaaa agaaaaaatc ttattcttga taacaagcta cgtgacgtat
     3961 atgcctaaca aaaatagaac ttttgaatca atcttaaatg aaggtttaac cacagaaccg
     4021 gcatccatta tttttgatga agttatcgat gacgttgaag atagtaagta tcataaaaat
     4081 ttaaaagaac ttttttattt tgcactcaac aatggaccaa gtttgttcac tggtatatca
     4141 gtacctgata gtgataatcc attaattatg gcaaaaattt acttaacaaa atggtgtaat
     4201 aaatacatca gggataggaa taacccagca ttaaaaaaac ctcttaaaac atttggcgaa
     4261 aaagatgctg ctttaactac tagagtagca gcaaatgcta atattgatga tcaaaaagta
     4321 gtgaatgact acttgcgtgg acatttttta tatatgagcg ctgagaacat gaacggaagt
     4381 attttagagg aatatttagc cgaagtgtta gaacctgaag gttggatttg gtgcgccggc
     4441 tctgtatatc gagctgttga tttctgttat ttaggaacct caccaattct tttacaagtg
     4501 aaaaataaat acaatacaga aagcagttcg agttcagcta taagagtagg aacaacaatt
     4561 agaaaatgga atcgtttaaa taagccgaca aaaatttctg gtttggatag ccctatcccc
     4621 aattggaaag cattaataga aatgactgaa gctagtaagg aactggcagc taagttaact
     4681 gaaaatagtt atttagctta tattaatgaa aaatccacac gagaattatg gacactcgat
     4741 gactaacaat atccttatta gtatctatcc acgatacatg gaaaaaatta taaacaaaca
     4801 aaaaaacttt gaatttagaa atttctatct tacctcagat ccttataata tcttttggat
     4861 ttacgaaaca atccccacca aagctttaaa gtataaattg attgtaaaaa atccaattac
     4921 taaacttaag aaaaatcaac actacttttt aggagacgat gagtttgaac aattagttta
     4981 aaagggaaaa tatgcttacg aaattatcca cctagaagaa atacttttac caataagtct
     5041 gtctaattta aaaaatttag gagttactgc accacaagga tatgcatata taaaaaaata
     5101 tcctagtctt gtcgatattc tcgccgtttg tataattaag cgagacaaat aaaaaagcca
     5161 tttatgttac actcaaattg taatacccgc taaagcatac aaggagagtg aacataaatg
     5221 acttataccc attttacacc aaacgagctt gtaatgatag aagcatattt tcatcaagaa
     5281 actccggttg ctatcgttgc gaagcagctt aaacgtggac gccaaacaat ttacaatgtc
     5341 tataactttc tcaaatgtgg tggaacagca cttgaatacc ttgaacaata caaagaaaat
     5401 aagcgacgtt gtgggagaac cgaaatcatt tttcctgctg aggaaaaaga atacattgca
     5461 aaaagatcaa ctgaaggttg gaccccagac gtcattattg gccgtgcaga gcgaaccttc
     5521 tcttgttcag taagtaccct ctatcgccgg tttaagacgg gagaattcaa tgttttacat
     5581 ttaccgatgc aaggaaaacg aaaaccaaat ggttattatt tgtcaaatta agcgagacaa
     5641 aactttccat ttacgtaact caaaaaaact tctaatggtg tttggtaatt tagtgatttt
     5701 ctagggatat ggtttctttt ggacgcaacg gatgagatga atccttgatt gacttggttg
     5761 aa
//
LOCUS       DAZUMK010000146         5743 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000146 DAZUMK010000000
VERSION     DAZUMK010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.149"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            328..1251
                     /gene="truB"
                     /locus_tag="ACG808_002581"
     CDS             328..1251
                     /gene="truB"
                     /locus_tag="ACG808_002581"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFS5048170.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1255..2196
                     /gene="ribF"
                     /locus_tag="ACG808_002582"
     CDS             1255..2196
                     /gene="ribF"
                     /locus_tag="ACG808_002582"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFS5048171.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2591..3376
                     /locus_tag="ACG808_002583"
     CDS             2591..3376
                     /locus_tag="ACG808_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS5048172.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3389..4240
                     /locus_tag="ACG808_002584"
     CDS             3389..4240
                     /locus_tag="ACG808_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFS5048173.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4364..4816)
                     /locus_tag="ACG808_002585"
     CDS             complement(4364..4816)
                     /locus_tag="ACG808_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5048174.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(5281..5649)
                     /locus_tag="ACG808_002586"
     CDS             complement(5281..5649)
                     /locus_tag="ACG808_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFS5048175.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
BASE COUNT         1937 a          931 c         1163 g         1712 t
ORIGIN      
        1 ttaagaaact gaaaaaataa ataaaaaaaa acggaaaaaa gactggaaga atggacagtc
       61 ttttttttcg tttttgggaa gtgaaaacca aactatttac ctgtaagcca cccataaatc
      121 caatcaaatg gtgcaaagaa aaaatcttcc attctattcc ctcctttaaa aaaaacataa
      181 catgatttct atttacgtga gcatgaattt tgttacgttt ttattgccga aaaataaaaa
      241 ccgttcatta tgatgtcaaa ttcgtcagta attgatgcaa tgtgttataa taattcagtt
      301 aaatcaaaaa tgaaatggag aaaagacatg gacgggttat taactttatg gaaagaacgc
      361 ggaatgacaa gtcatgattg cgtattcaaa ttaagaaaaa tcttacatac gaaaaaaatc
      421 ggacatgggg gtacgcttga cccagacgta gacggtgtgt tgcccatttg tattggaaaa
      481 gccacaaaag ttatcgaata tttgacagat tctgggaaaa tctatgtagg cgagataact
      541 ctaggtttca gtacaacgac ggaagatcga tccggagaac gggtagaaac aaaaccagta
      601 gatatgtctt tatctgaaga aaaaatagat gaagtgatgg cttcatttat tggtgagatc
      661 acccagatac caccaatgta ctcagcggta aaagtcaacg gtaagcggtt atatgaatat
      721 gcccgcaata atgaaacagt ggagcgtcct gttagaaagg ctcagattta ccgttttatc
      781 cggacaagtg aattgacaag agacgaagtg acaggaacca tttcttggcg cttcgaagta
      841 gaatgcggga aaggaaccta tgtccgcaca ttagctgtag atacaggcaa gaaacttggt
      901 taccctgcgc acatgtctga tctgacccga acagcaagtg ccggtatgtc ccagaaagat
      961 gctctgacat tagaagaagt agctcaagct gtgaaaaatg ggacaatcga tgattgtttg
     1021 ctgccaatag aaaaaggagt gaaacgattc aaacgagtag atattactga tgaagtatgg
     1081 cagaaagtaa aaaacggtat gagacttaat tatactgtat ttgcgctccc agaaatgccg
     1141 tcagaagaaa ttgctttgtt ttataaaggc aaagttgtca gtatctatca gccaaacccg
     1201 aaagaaaaaa acaaattaaa acctagtaaa gtattgagaa atgaggaata acgaatgaat
     1261 atcatcaaga tcagacatcc ttaccaatca gaacaaattc cagaagacga tgtcgtttta
     1321 gtactaggat tttttgacgg cgtgcatatt ggacaccaga aagtcataaa gacaggtatg
     1381 gaaattgcac aaaaagaagg attgaaatta gcattgatga ctttcaatca gcatccatcg
     1441 attgtcttta aaaagatcga tcctagcagc gtgaaatatt tgactagcct taagcaaaaa
     1501 gaagaaaaaa tggccgcttt gggtatagat tatttatatg aagttgattt tacttcttct
     1561 tttgctcatt tagcaccgca ggaatttgtt gatcaataca tcgtagggtt acatgcaaaa
     1621 tatgctgttt ccggttttga ttatacgtat ggaccaaaag acattgcaga tgtcgctcat
     1681 tttcctgaat atgccaaagg aagattcaaa atcgtcacag tttctaaaga agaagaacaa
     1741 ggtgaaaaaa tcagctctac ccgtattcga ggattactag acagcggaca agtaaaagaa
     1801 gcagcaaaac tattaggttg cacatatgag gtagaagggg tagttgtcca tggcgatgca
     1861 cgaggcagac tactaggatt ccctacagca aatgtcaaag taaagagcac agttcatttg
     1921 cctaaagaag gtgtttatgt atcggaaata aaaattgggg aaatttggta tccagccatg
     1981 ggttcaattg gacataatga tacattcggt gaaggacggg agctgactgt agaattgtat
     2041 attttggact ttaatcaaga tatctatggt gaacatgtcg cagtacgctg gaatcaattt
     2101 ttaagagatc aagtgaaatt ttctagtgta gaggaattga ttgaacaact caaagaggat
     2161 gaacaagcaa cagctgaata tttttctcag caataaaaca ttatgcataa aatctcttgt
     2221 ttatgtataa ctagcaattt caataaaatg ggtgataaat aagcccagga aaggggctgt
     2281 gacaaaagtc ttgtcacagc ctttttttcc aaataaatgg tttttcagaa gtagcttctt
     2341 cggaaataag ccgaaatttc acaaaaattt gagaagtaat ttttgtaaat tacttcttat
     2401 ttcttgaagc tggccacttc tgtcacaacc tctttcttta tagaaataaa aggacactag
     2461 caattcatga aaacgattgc taaagcgtat catttgcttt agactatata tcttggtgta
     2521 cactcacttt aagacggaaa taaaagttat ccgtaataac aggtttaggt atgaaaaagg
     2581 agtgggtact atgtataagg aattagaaaa caaagtcgca gtagtaactg gtggctcaaa
     2641 ggggattgga acagcgattg ctcaacgatt cgggaaagaa aaaatgaatg tagttgtcaa
     2701 ctataactcg gataaagaag gagcagaaaa agcagtcaaa gcaatcgaag aggcaggcgg
     2761 aaaagcagtc tctgtacaag cagatgtcag ttctgaagaa ggaatacagc gtttacttga
     2821 tacggcttta gaaaattttg ggacactgga cgtttggatt aataatgctg ggatggaaaa
     2881 tcagcatcca acccatgaac ttagccttga agattgggaa agagtattaa aagtcaattt
     2941 gactggcgtt tttatcggaa cgaaagcggc tttaaattac tttacagaga atgataaaaa
     3001 aggaaatatc atcaatatgt cttctgttca tgaacaaatc ccatggccaa cgtttgctca
     3061 ttacgctgct tcaaaaggcg gagtaaaact attcaccgaa acggtagcaa tggagtacgc
     3121 accaaaggga atcagaatca atagtatagg acctggtgct atccgtacac ctattaatgc
     3181 ggcaaaattt gatgatcctg aacaaaaaga actcttggaa agtatgatcc cactaggacg
     3241 cgtaggtgaa ccaaaagaaa tagcggcgac agctgcatgg ttggcttcag acgaatccag
     3301 ttatgttaca ggtattacac tatttgcaga tggtgggatg actttatatc cttctttcca
     3361 aggtggaaaa ggataagggt gaataaaaat gggtttattg attgcattga tcccggcagt
     3421 tgggtggggg atccagccac tgattttgaa aaagattggt ggcagaccaa caaatgagat
     3481 attaggaact ggtattggag ccgtgctcgt tggcttactt gttcaattat tcgcttctcc
     3541 agctggtatt tcagtgacta catttttgat tagtttactt tctggtgcat tctgggtcat
     3601 cggacaaaca ggacagtata catctttcaa cttgattggt gtttctaaaa cgatgccaat
     3661 ttcgactgca ttacagttag tcggaacctc attgattggt gtcttcgctt ttggagaatg
     3721 gtctggaagt atgggaaaaa tcattggtgc agcagcaatc attcttcttg tatttggttc
     3781 tgctttaacg gcaatcagtg atgatcctgg aaaaaaaggc ggtatcacaa aaggaatcag
     3841 tctattagca tcaacaagta ttggttattg ggtttacagt gcattgccca aactagttga
     3901 tgcaagcgga ctttccattt tctttccgca aatgatcggt gtctttttag gggcagtgat
     3961 ttatgtgatt ttccgaaaac caaccgcatt ttctgaagac aagagttgga aagcaacaat
     4021 agttggagcc atcttcagtg tgtcagcatt ggcttatatt ttttcagcaa atgaaaatgg
     4081 ggttgctact gcatatatca ttacacagct aaacgtagtt atctctacat taggcggtat
     4141 gtacatcctg catgaaaata agacaccaaa agaaaaaaaa ttaacgatta tcggattatt
     4201 gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat ggtaaaatct
     4261 catttattaa aaaaatattt gtttgaacca aacagtaagt acttataaaa aaatagcgct
     4321 agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat acaagtgaaa
     4381 tatctgtctg catcaataat ttttcattga tccattgact aaaatttacg acatgattga
     4441 agttaccacc gattagtatc tccgcatgat tgtctttata aagatcgtat tgtaaaagac
     4501 ggtctttttt atttgcgtaa acatcgttat ggtactggac taattgttct cggatttgat
     4561 tgattgctgt aaaatcacga tactgaaggt tgaaacgcat cgtgattggt gcatttagca
     4621 tgaatcgctg catttctttt gctgcttcct ctgcttgatc tccgtatagg aactcaaaaa
     4681 gattacgttc agaaaatttc cattgcccgc caacctttgt tccttttagt tttccttcat
     4741 gaagataatt tctgatagaa cgtgtagtaa cacctaattg ttcggctaat tcatttactg
     4801 agtaaaaatc tttcaatgtt caccctccta tgtgaatggt tctactgact tagttgatat
     4861 ttgcttaatc acgaaatgta gaaagaacga ttttaattaa ggtcattgta aaaatcaagt
     4921 cagaaaattc taacttttct ttctctaaag aaattcgttc tctcctaaag tgatattata
     4981 tttaaatata cctgaaaata atgggcagta ttatgaaaga tcactgatat tttctcgtat
     5041 tttttcctta ttcaaaagtt ttgcaggaaa attgctgaaa aaataatttt actaagtgga
     5101 aaattcaaag ccattcaatc agtgattttt ttcagcacaa gaaagactcc ttataaaaat
     5161 agcagatact ttgtcaatta agtgtttaac cagggatgat aaaaaggata ggattgaaca
     5221 aaacctgtat aagctgatga atagtttcct cgccagtaaa aggtttattc gtataaagct
     5281 ttatcgccaa aatttatcat gacctttttt atcttcaata ttaaatgaaa tcaattgttc
     5341 aatcaattcc cagttgatta cacttttcca aggaactttg atgacgttat ccgtatatac
     5401 gtagcctgct tcttggatat ttttttcaag ggcacgaatc gctgcttttt ccggtgcgat
     5461 agaaaaatga tttttagcaa aactaaatgc aataataaag gtaccatggt cagtaaacat
     5521 aggttggttc cattttacag ttgtgtctag ctggggatac gtttctgtta tcttagcaaa
     5581 aaggtctttg atcttttgtt gatgttcttt ttctttgatc tgattgatga tcggctgaaa
     5641 tttttccatt ctaaaacgcc tccattaagt ggtttgattt attttaccag aaaaacaata
     5701 gttatgtata aaaaacgaac aaaacacaag taatgttgta acc
//
LOCUS       DAZUMK010000147         5725 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000147 DAZUMK010000000
VERSION     DAZUMK010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.150"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            195..920
                     /locus_tag="ACG808_002587"
     CDS             195..920
                     /locus_tag="ACG808_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048176.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            910..1419
                     /locus_tag="ACG808_002588"
     CDS             910..1419
                     /locus_tag="ACG808_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048177.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(1489..2937)
                     /locus_tag="ACG808_002589"
     CDS             complement(1489..2937)
                     /locus_tag="ACG808_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5048178.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2937..3653)
                     /locus_tag="ACG808_002590"
     CDS             complement(2937..3653)
                     /locus_tag="ACG808_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFS5048179.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(3634..3984)
                     /locus_tag="ACG808_002591"
     CDS             complement(3634..3984)
                     /locus_tag="ACG808_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFS5048180.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            4128..4901
                     /locus_tag="ACG808_002592"
     CDS             4128..4901
                     /locus_tag="ACG808_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5048181.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            5072..5438
                     /gene="ssrA"
                     /locus_tag="ACG808_002593"
     tmRNA           5072..5438
                     /gene="ssrA"
                     /locus_tag="ACG808_002593"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(5484..>5725)
                     /locus_tag="ACG808_002594"
     CDS             complement(5484..>5725)
                     /locus_tag="ACG808_002594"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048182.1"
                     /translation="KNLFEGTCLNHNCVFPSISIKVHLKKSVRTKVLFVNLKIDFPEY
                     VLGISKCLNVFLNAKSMQKILYFSTFLSLTKIKKR"
BASE COUNT         1660 a         1124 c          994 g         1947 t
ORIGIN      
        1 aaattattgt cgctgctcct cttttcgaat aaacagtgtt caaaagcttg catctacggt
       61 aatagaatag ggcagcttct tcacaacctc ttgcttttta caaaatattt tctggtttta
      121 attcgtgatt tcactaaagg aatttcatac tttattcata ttcattttct atacttttta
      181 tagaggtgaa aagtatgaaa ataatccgct atgctactgc tcgattaatt tattacaaaa
      241 agcagacaat actctattgt ttattttcaa gcttttctgc ttttttattg atagcatgcc
      301 tgactcttta ccattcgcaa tcggctttag ccaatcaagt ccaagatcgg ctttcattta
      361 ttggcgcttc tgcatctaca gagcttcttc cggctttaga attagctagc cgattttata
      421 taaaaggcat attcagttta ttttttctat ttgccttcct ttttatcttt ttcttttact
      481 attcagtcag gcaaaacaag caggaattga tcaattggcg cctgactggt ctttctaaaa
      541 ccaaattatt cctttttatt ttctggcagc tgttctttcc tctctttctt tgttgtatct
      601 tggttttatt gtggatcgtc agcttccagt cagtttatga agcgcttctt caaaggatca
      661 attttcttgt cttaaagact actgaactgc cagatatccg tgatatcttg acttcggtaa
      721 gcggacttac aattccgatg gatcaacaga ctttttttac catcgatttc caaaatgatc
      781 tttttttcac agatatactt aagggatttg cccaaactct tattgggctc agtggatgct
      841 gtctcattct tagtctattc cctttcactt tttttatcca tcatttgcaa agtaggaggc
      901 ctggacgtaa tgaaacctga atatttgatt catgctgaac acttgaccgt tattttgtgt
      961 cctctcgaaa aacagcataa aatattggag cacttgaaaa aagaggttct tgttgcaaaa
     1021 ctggataatt ttgcgatcat ccaaaaaaat tggccgatgt ttccttatct gaatcttaaa
     1081 gaccatgtcc tgttggatgt accggaaaaa gaaatcaaac aagatcgttt ggcctatcaa
     1141 gaaaaacttg atatctctcc ttccttgctt aattgtgcgg cagctgagtt gacgtctttt
     1201 gaaaaagtaa agctgcaatt acttcatgcc ttgctttcca agcgaaacaa tattgttatt
     1261 gaagatacat ttgatgaact tagtgtattg gagatccaag agctactcca cttgctttct
     1321 tatttagctc atgaagaaaa tcaaggtatc ttactcttta ctcatgatgc tactattgct
     1381 caaagtcctt atattgatcg tttagagccc gcgggctaaa tcaatcattt tatagtaaaa
     1441 acaagcggta tagaagatcg attcatgatc ttttataccg cttgtttttt actctttttt
     1501 gatttctacg tcaggcaaac gaacagcgaa ttttgttcct tttcctagtt cgctttctac
     1561 ttctaatttt cctttatgca agcgtacaag ttgctgcacg attggtaaac ctaatcctga
     1621 ttcaccatat tttgtatttt ttcttgaagg atctacttta taataacgat cccagatact
     1681 tttcatttgt tgttcagaca ttccaattcc cgtatcttca atcgtgatga tcgtttcaag
     1741 atagcctttt tctaaagtaa tcgtgatttg gccgttttca gtaaactgta tcgcgttttg
     1801 aataatattg accatgattt ggacaaatcg gtcataatca gcgtaaacat caatatcatc
     1861 aacggctttc aatgtcaatg tatctcctgc tgcttctgct ttagcttcta actgtgcaac
     1921 aatatttttc aatgtttctg ttccattgaa ttttttaatg accatactga tttgattcgt
     1981 acggattttt tcataatcca agttttcatt caccaaacgg atcagacgct ctgtttcatt
     2041 tttcatcaat ttaatcgcgt tttctcgctg attttcaggg attgcattgt attctaaccc
     2101 ttccagaagt ccgttgattg tcgtaagagg tgttctcatc tcgtgagaag catctgccat
     2161 aaattgtctt cgacgatttt cttgttcttc aatttctgct tgagattctt tcaatgaatt
     2221 cgtcatttta ttgaaatcat ctgctaactc atcaaattca tctttgtcat gtacggggag
     2281 ctgtacatca aaattaccat tcgtcacttc tttcgtcgca ttccttaacc gattgatacg
     2341 cttaacttgg aatgctgcaa aagcatagct ggcaataact gcaacgatac tggaaaaaat
     2401 aaatcctttg aacaggttca gtgtaattgg acgaacgctg ttatcgatat ttcgcgcagg
     2461 ttgactaact acaagtgctc catagaattc atgattcaaa ttaaatggca caagtgcgta
     2521 agaggtcgcc tgtgcttgtc ctaaaatatt tttattcgat gtgaattttt cttgacgtcc
     2581 attcctcaga tttttccatt gatcagctgt taaggtaaaa ttcagtaaag ctgtattagt
     2641 cggataaatg acacgttcgt ttttatcgat aaatataaag ttcacatttt gttctgtcag
     2701 tacttgctct gtcagcagca atgcattttg aaacgcctga tcttgactaa actgcgggag
     2761 cgtatcggca taggtttgcg ttgtcttttc aactgattca gcatatccaa acagttgctg
     2821 gtaattgttt tcttcaatcg tttgttttgt cagttgtgta aaagaaactc caacgatcaa
     2881 taagatcaac gcgaccattc cgcaaaaagc taagagttgc tgatacaaat aacgcattag
     2941 gcaacaccag aatcatcgaa tttgtagccg actccccaaa cagtttggat gacttgtggt
     3001 ccgacctttt cgattttttg tcgtaatttc ttgatatggg cgtccactgt ccgttcatca
     3061 ccaaagtact gataatccca gactaattca agcaactgct cccgagagaa tacttgacgt
     3121 ggtttttttg ctaaagtaaa caatagatcg aactctttag gcgtcagtcc ttcaattggc
     3181 cggttgtcca gatacgcttc tcgtgttttc gtattcatct tgaaatgact agtctcgata
     3241 tcaaaatgat catctgaaac tgctgcttct tctcttgctt ctgccaactc actacggcga
     3301 tgcaaagctt tcattcgagc aataagcgtc agaggactga aaggttttgt gacataatca
     3361 tctgccccca tctctaagcc gatcacctga tcgctttcag aatctctagc tgtcagcatg
     3421 ataatcggca cagtacttga aactttgcgg atctctcttc ctactagcat accgtccagt
     3481 gtgggcaaat tcaggtctag tgtaatcata tcccaatgtt gtgggttttc taaaaaggca
     3541 tcgagccctt cttttccatc atacttgaaa gttgcttccc aaccttcatt taagaaaaac
     3601 atctgcatca tttcagaaac agattgatta tcttcaatca ttaaaatatt cattatcgtt
     3661 ccctccagtt tccaggtcat atttctgtaa tttcttttct gtcataataa aaataggcat
     3721 aattggaaat actagcagaa gcatttcaat cagaaaataa ttcgtatgcc cagatgtctt
     3781 taaataatag ccgatcaatg ccatcagtaa gcccataaga aaaaaccagt ttccgcttgt
     3841 cttttgagcc agccgccaat gagcgtctgt ttttgcggca agaggcgaat gatacccata
     3901 aaaaggcaga tttccttttg agggcagaat ccgaaaaaga ctgccaatta ttaagaataa
     3961 gataccaata ctgaaaaaaa tcattctttc cctcctaaat tctcttctta tagtatactc
     4021 taaattgttg actgaaccaa tatgttcagg aattcttcac gaaaggtttg tcttttgttc
     4081 attctttgtt aagataaact tattccaata aaaaaggagt tttatttgtg tcaaattatc
     4141 gtgcaatcac tttctttgat cttgatggaa cattactaga tgcgaaatca aaaatcacac
     4201 ccgaagtggc aaaagctatg aacgcattaa aagaaaacaa tgttctcccc gtcatcgcta
     4261 caggacgtac agaagcagaa atccactcta tcatggaaga tgcgggcatt acttctgcgg
     4321 tcaccatgaa tggttctttc attcgagtag acggcgaaga aatctattct caaacatttt
     4381 cttcagaaga atgtcaaaaa atgtatgatc atgtacagtt gaacggtcat gaactttctt
     4441 actataacga tcagaaaatc tggtgtaccg gtcatagcca aacaatgatc aatgcttacg
     4501 acttcattca ttcagatgtt ccggaaattg atcctttagg ctttaaagaa aacacagtaa
     4561 atatgttgct gatcttatct caatcaggtg acgaatacta tcacgaacat tttcctgagc
     4621 taacttttta tcggaatggt ccttactcga tcgatacagt gaagaaagga atctcaaaag
     4681 gggcaggtgt caaacgtctg caaaaagaac ttcagctgga aaaagttcct acttttggat
     4741 ttggcgatgg tccgaatgat tttgccttgt tggaagcttg cgacaacaaa atcgctatgg
     4801 acaacgccta tgacggatta aaagaacttt ctacctttat cacaaagaaa aatacagaag
     4861 gcggaattgt ccacgcattg aagtattttg atctgattta agatcttttt ttgaagctac
     4921 ctcttttctt aagaaaatcg cttatttttt cttaagaaag gggcagttat tatataaata
     4981 atgggatatg attcgctttt ttattgaatt tatcccaata tatgctatac taattttgta
     5041 tgaaaaatac acgtaaccct tgttagctaa cggggacgtt acggattcga caggcacagt
     5101 cgagcttgaa ttgcgtttcg taggttacgt ctacgtaaaa acgttacagt taaatataac
     5161 tgctaaaaac gaaaacaact cttacgcttt agctgcctaa aaacagttag cgtagatcct
     5221 ctcggcatcg cccatgtgct cgagtaaggg tcctaacttt agtgggatac gtttcaactt
     5281 tccgtctgta agttgaaaaa gagaacatca gactagcgat acagaatgcc tgtcactcgg
     5341 caagctgtaa agtgaatcct taaatgagtt gactatgaac gtagattttt aagtggcgat
     5401 gtgtttggac gcgggttcga ctcccgccgt ctccatatat gtatggttga gacagataaa
     5461 catgtatgaa aaacgttgtt aaatcaacgt ttttttattt tggtcaagga gagaaaagta
     5521 gagaaataca aaattttttg cattgatttt gcattcaaaa aaacattcaa acatttagaa
     5581 atccccaata cgtattcagg aaaatctatt ttaaggttta caaaaagaac ttttgttcgt
     5641 acactctttt tgaggtgaac ttttatgctg atggaaggaa aaacacaatt atggtttaag
     5701 catgttcctt caaataagtt ttttt
//
LOCUS       DAZUMK010000148         5575 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000148 DAZUMK010000000
VERSION     DAZUMK010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.151"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2088)
                     /gene="ftsH"
                     /locus_tag="ACG808_002595"
     CDS             complement(<1..2088)
                     /gene="ftsH"
                     /locus_tag="ACG808_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFS5048183.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDN"
     gene            complement(2178..2723)
                     /gene="hpt"
                     /locus_tag="ACG808_002596"
     CDS             complement(2178..2723)
                     /gene="hpt"
                     /locus_tag="ACG808_002596"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFS5048184.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(2723..4168)
                     /gene="tilS"
                     /locus_tag="ACG808_002597"
     CDS             complement(2723..4168)
                     /gene="tilS"
                     /locus_tag="ACG808_002597"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFS5048185.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(4288..4758)
                     /locus_tag="ACG808_002598"
     CDS             complement(4288..4758)
                     /locus_tag="ACG808_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFS5048186.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(4804..5229)
                     /locus_tag="ACG808_002599"
     CDS             complement(4804..5229)
                     /locus_tag="ACG808_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFS5048187.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(5296..5565)
                     /locus_tag="ACG808_002600"
     CDS             complement(5296..5565)
                     /locus_tag="ACG808_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFS5048188.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
BASE COUNT         1420 a         1244 c          962 g         1949 t
ORIGIN      
        1 attatcttct gattgttgag aatctgaatc atgttctgct ttattttcct cattcagttc
       61 atgttttgct tcttcaaaat cacgtttttc ttccgcttgt ttttcagcgt ctttttcttc
      121 caaagcacgt tttgcttctt caaaagtttt cgcttctttt tcgcttggat aatcatttga
      181 ctcagtacct tcaggcatca caccgtgttc aaacaatgac ttgattgctt tggcatccaa
      241 tgtttcgaat tccaaaagtt tttcagcaat cagtttatgc tgtgcacgat gagcttcgat
      301 gatttctcga gctttatcat gagcttccat caagattttt cgtacttctt gatcaatctc
      361 gaaagcgact tgttccgagt aagcttttgt ttgaccatag tcgcggccaa caaacacttg
      421 atggtttcct tcatattgaa caggacctaa acgatcactc attccatatt ctgttaccat
      481 gcttcgtgct agagctgtcg cttgctcaaa gtcattggaa gcaccagttg attggacatt
      541 gaagatgatt tcttcagctg tacgtccacc aagtaagcct acgatttgtt caaacatatc
      601 ttctttcgtc atcaagaact gatcttcttt tggtaaagcg atcatatatc cgccggcgcg
      661 tccgcgaggg atgatcgtta ctttgtggac gacccgagcg cgactcaata ctaatccgac
      721 aatcgtatga ccagcttcgt gataagcaac catttcgcgt tcttttttgc tgattacgcg
      781 atctttttta gctggacctg caatcacgcg atcttccgct tcatcgatat cagaagcatc
      841 gattttcttt ttatttcttc tagcagctac taacgctgct tcattcaaga cattttctaa
      901 atctgctccg gcaaaacctg gtgtctgttg agccacaact ttcaagtcaa cgtcatctgc
      961 catcggttta ttccgtgcat ggactcgcaa gattgcttca cgacctttta catctggacg
     1021 tccaactaaa atctggcggt caaaacggcc tggacgtagt aacgctgggt ctagtacatc
     1081 agaacggttg gttgcagcaa tgacgattac tccttcattt ccgtcgaagc catccatttc
     1141 tacaagcaat tggttcaatg tttgttcgcg ttcatcatgt ccgccgccca taccggcacc
     1201 acgttgacga cctaccgcat caatttcatc aatgaaaatg atagctggtg cgtttttctt
     1261 cgctgtttca aacaaatcac gaacacgact tgctccaaca ccgacaaaca tttctacgaa
     1321 atcagaaccc gagatcgagt aaaacggtac tccagcttct ccggcaacag cttttgctaa
     1381 taaggtttta cctgttcctg gaggtccttc taaaagtaca cctgccggga tacgggcacc
     1441 taattcaaca aatcggcgag gatctttcag aaattcaaca acttcaacta gttcttgttt
     1501 ttcttcttcc gcacccgcta catcagaaaa acgtacgcga ttagcttttt tgtctgcttc
     1561 tttggctttt gatttaccaa agttcatgac acggccgcca ccgccaccgc cgccgccttg
     1621 ttgtcccatc atcatgtaaa agaaaaagat gatgattacg attggcaaga agctaagcaa
     1681 tagggaaacc catataccgc tgcttgattc ttcttttact gttgtagcaa ctttgttttc
     1741 ttttgccagt tctgttactt ggctcaatgt tgtatcattt ggcaaaatga ttgttgtgaa
     1801 atgggtagta gtcgtatctg ttgaacctaa gatagctaac ccaccgctgt tagcgacggt
     1861 ctgctgttct ttgtattcac cagtgatctt atatacacca tttgcaggct gcacttcaaa
     1921 ttctttgatc ttgcctgctt gtagttgttc tgtgaatttt gagtactcga tatctgacga
     1981 tcgctggccg ttattaccaa aaatgaaata aacgaccatt accatcgcta agattacgag
     2041 gacataatac aaggcatttt tcatcatgcc gttgttcttt ttgttcatag ttgccctccc
     2101 taagcgtcaa tttaatttaa cagtttttta tcaatcctga ccattctatt gtaccataat
     2161 ttaattgaga tacagcatta atttgattga taaacactag gtttcaatac accgatgtac
     2221 ggaaggttac gatatgcttc tgcataatcc aagccataac cgaccacaaa ttcatttggt
     2281 acgttgaagc caacataatc tgcttcgata tttactactc ttccttcagg tttgtcaagt
     2341 agtgtaacaa ttttgactga ttttgctttg cggtatttga aaagatccac taaataagca
     2401 agtgttcttc cgctgtcaat gatatcttca acgatcaaca gatcgcgacc ttctacattc
     2461 gtatctaagt ctttgacgat ttttacttcc cctgacgaga ctgttgcatt cccgtagctt
     2521 gaaacatcca tgaaatccaa ttccaaatac gtatcgatag aacgaacgat atctgccatg
     2581 aatggtaccg ctccttttaa gacacctact actaggggat tggtgttttg gtaaatttct
     2641 gttaactctt tgcctagttc tttgcaacga acttgtatct cttcttgtga gatcaaaatt
     2701 ctctcaatat ccttttccaa catcaggttc tccttccaat tgcttctttt tgatatttgt
     2761 aaagaagtat gtagtgtatt ttatcagttt cttcacgaat actcaaatat gaaaaggcaa
     2821 aaggtatcaa gctatatata tttcttttgg catctgtaat gatccaagca tgctgccttt
     2881 gtgaattagg tactttctga tcgataaaat atctagatac tttttttgtg agattctctg
     2941 ttaatttaat gcggtctcct gcttttcttt tttcgacata cagttgcaca tcagcaggaa
     3001 tccaaatgtc atgagaaaat agagaccatg aatctgttgc tttgatgtcc cctgcatttt
     3061 caggtttgac cagaccaatc cattctgttt cattcaaata tattccttgt tctggaatca
     3121 gcaacaagcg ctggtcttct gtctggctag ttgcttgttt ttctttttta gagagtacaa
     3181 caaaatgata ctctcttttc agtatccaat tattgccaat actgagttgc cattggcttt
     3241 ttttctgttg ccactggtct aaaatcgact ccatttgttg ttctttcaaa ataattcctg
     3301 tttttgagaa tgctttgtcg aaaaaggagt aaaaaaaata gtatcgttca gcatctgtca
     3361 tttcttcaat catttctgca gaaaatcgaa attgttcctc ccactgttcg acttcctttt
     3421 cgatcaactg tcccatatat ttttgaatga cttggtctgc ccattggatc tgttgcgaaa
     3481 attgctggaa atgtctcaac gcctgtgtat tttcttgttt caattgagga actaccaaat
     3541 gacgaagccg atttctctgg atatctaaca attggtttgt ctgatcctca aagtaaacaa
     3601 gctgcgattc cgctgcataa gtatataggt cttctttgct aaaactcagc aacggacgaa
     3661 tcagtctgcc cgtagcaaat ggttgtactt ctttgattcc agaaaaagta tttaactgtc
     3721 ccccacgaac gattttcatt aacaccgtct cgatttgatc atctccgtga tgagcagtca
     3781 tcaacgtgtc atattgtcct tcttccatca cgcgagtgaa ttcttgataa cgaaagttac
     3841 gagcttctgt ttctatggcg gatttggctg gatcttccca aatacttaca tataaaggga
     3901 gattcttctc ttcacaatat gagcgaagat aggcagcttc ttctgctgat tcttctctta
     3961 attggtgatt gatgtggacg atacctaaag aaaaaccgat ttggcgttgc acttgtaaaa
     4021 gtaaatggag caaaaccatc gaatcaacac cacctgatac cgctgctaaa attctcgatc
     4081 cttgttgcca gaatccttgt tcattcccgt gccgaataaa tgactgctgt aatagcggtg
     4141 cttttttccc gcctttttca acgttcatcc tttccacacc ctttcttcct aaaaaaaaga
     4201 gagcgggaaa acgtctaaaa cgacaattaa cctgccctct gttgatacta tgacccaaat
     4261 agtcgcacta tcattatttg cggcttttta gctgcgacgt ccgccacggc caccgcgctt
     4321 tccttctgtg tttcgcttca atgaagataa gcggtcatcg ctatctttca aaaatgaact
     4381 catcaatgaa tcgaagtctt ctttttgagc tgaaggttga ctgcgagaaa aagatttttt
     4441 ctgtgctgga cgatttcggt tgtcacgggg atctttttgt tgatattttt taggacggct
     4501 ttcttctcca gattcagcag gttgttcctg cgctttccgg atagatagtc cgatttttcc
     4561 atcatcattt actgaaataa ctttaactgt tacttcatct cctacactta aaacatcatg
     4621 gatgtctttt acatagccat ttgacacttc gctaatatga actagacccg tttttccagc
     4681 acctagatca ataaacgcac caaaatttgt gattcctgat acttttcctt gcagttttgc
     4741 tcctacctcg attgacataa aaaaaatgtt cctcctaatt atttcccttt tttatattcc
     4801 tgcttatttt gcagttgtag aactagttga ttgatctgtt tcatttgtcg agctgtctgt
     4861 atttgattct aatataggat aaactttttc accgtctttt gagtaaaaga atttgcttct
     4921 ggcaagttta gctacatact catcatcttt cagcaatgaa acatcttggt gcagctgatc
     4981 aacgtctgcg ttgacttctt tcatttcagt atttgcttcg actctcaatt cgttcaattg
     5041 atgcatcctt tgcatgtcat ggaaaatctg cacacctatt atcgcgaaaa tgaccaaagc
     5101 accaacaaaa attgcagcca gtctgcgtct tcgaaaaatc agttgacgtt gttgttgctg
     5161 aaattcagca tactttttct ttgcatattc cgtatctagc gcagcgattt tattgctttt
     5221 tttcgtcact tggattcgct ccaatctttt tgattgtctt tttcattata ccaatatcgt
     5281 gaaagcttgt ctaatctatt tctctagtgt ttctgtgcga ttttcagaaa taatttcgta
     5341 cattttttgt gcatcttctt ttttggttga ttcatgtaat tccgtaattt tcacttccaa
     5401 cgttttgttg ccaaactgga ctttcaaaat atcgcctact ttgacatcac tggaagattt
     5461 cgccaattta ccgttgattt gaattcttcc tttatcagct acttcttttg ctactgatcg
     5521 tcttttgatg attcgggaaa tttttaaaaa tttatctaat cgcatatctt attcc
//
LOCUS       DAZUMK010000149         5483 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000149 DAZUMK010000000
VERSION     DAZUMK010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.153"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..666)
                     /locus_tag="ACG808_002601"
     CDS             complement(<1..666)
                     /locus_tag="ACG808_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5048189.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLIGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFK"
     gene            727..1467
                     /locus_tag="ACG808_002602"
     CDS             727..1467
                     /locus_tag="ACG808_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFS5048190.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(1553..2311)
                     /locus_tag="ACG808_002603"
     CDS             complement(1553..2311)
                     /locus_tag="ACG808_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFS5048191.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(2308..2772)
                     /locus_tag="ACG808_002604"
                     /pseudo
     CDS             complement(2308..2772)
                     /locus_tag="ACG808_002604"
                     /inference="COORDINATES: protein motif:HMM:NF023225.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
     gene            complement(2791..3288)
                     /locus_tag="ACG808_002605"
     CDS             complement(2791..3288)
                     /locus_tag="ACG808_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HFS5048192.1"
                     /translation="MILLLGFILWQTPVHASEFNFAVDFELPANQIDKNKTYINVRME
                     PGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESKSKFDETLSYDMKNLAK
                     TVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKENVSNKSTVGVVNQFSQV
                     KAILI"
     gene            complement(3383..>5483)
                     /locus_tag="ACG808_002606"
     CDS             complement(3383..>5483)
                     /locus_tag="ACG808_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFS5048193.1"
                     /translation="KINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDE
                     NEGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPE
                     NQLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATK
                     IVVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLS
                     QPFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKL
                     VLTKENQIAGNYSAEIT"
BASE COUNT         1555 a         1132 c          972 g         1824 t
ORIGIN      
        1 tttgaattcc tctagatgat ggtaatattc ttcatttatt tgatacagtt gaagcgattc
       61 ctgatgttgt ctttctttta cttggaaact gatcaacata atggaaaaaa acaaagaaaa
      121 aaaactgatg actatcgaca aaagagatag aagcagataa ttagatgagc ggaagtgaac
      181 acttcccaat accaagtaaa tcacgcccca gcttaataaa atacaaatac ctccaaaata
      241 gtgttcagtt ggataagtaa aaagcaagtt gagaaatctc gtctgtttgt ctatactttt
      301 tactaataga tagaaaataa atgcgataag tagtgcaaaa aaacttgctg ttccaagatg
      361 tctctttcct aaataatcca tcagatcaaa aaccagaaaa aagctgagtc cagtgatagc
      421 aaaaaaaaga ttactgatca aacagctgat aaacaggttt ttcgtagtaa aataaaaaag
      481 aaacatcaat aataagccaa ttaggtaagg aaagctcact ttagggatag gaagtaaaag
      541 tgtcaccacc atcaaagcta tacaaccaaa gctgtttttt actagcagtt gtttcgaaaa
      601 gaataaatag atacctaaat ctaaaatcaa agtaaaggtc aaataaagtg tcactgttgc
      661 gaccatctta ttcctccatt gttttgtttc aaaatgtaca catatttttt taataggagc
      721 ctgcatatga aaaagtattt tatcatagaa gataaccagt ttcacagcga atggatcatc
      781 agagaattgg tcaatcatca tatcgagaat atcgtgcagc tcacttctat ctctcaaatc
      841 aatcagttga tccagttggg cactattgat aatcacgatg ttttctttat cgatatcgac
      901 ttgaagcaat cactcacagg tattgatgtc gccgaacaaa tacgtaaaaa aaatgcacat
      961 agtaatattg tttttttcac cgcttatacg aattttgcag tagagatcat caatcggaaa
     1021 atccttccga ttggatatat cagaaaagac aggaatttat ctgaacaaat tcgtcaagtg
     1081 ctagaacaga tcaattggaa agaacagcag atcgtaaatg tcgataaaat ctttattgat
     1141 caaaaaggtg gaagtctcgt cctgtatcct cgggaaattt tatatattga aagtatcaaa
     1201 ggtttgaaaa accaagttat ggtcaaaaca actacagaag ataaattagt gaatacttct
     1261 ttaaaacaat tgaagacaga gttagaaaag tatgcttact ttttactttt aaaatcttac
     1321 tgtatcaact tatcgcaatt gcagaaaatt gattctgctc attatgtgtt agaatttttc
     1381 aacggtgact ccttactcgt tggtcgaaag atctttgaaa agacaaagga acgtttccat
     1441 gacttccaaa aaactgcttc gagttgatga ataagagctg agacgtaagc atagcagatt
     1501 tcagggagtg ggccttgaat cggctccact ccctgaagtc gttgatatga ttttacgctt
     1561 cctcttctgt aggagcatct gttaacgacc atgtgaatgt tgaaacgtag ttgtctacag
     1621 cataattgga tacaggaaca aataattcca ctgattgtct cccttgttcc acattctctc
     1681 ctaatgaata aatccatgtg ccaatccctt catttgctcc tgcagttact agatctactg
     1741 cctgatctgc tgggatttcg attccttcag cgccagttcc agaaacggta gatggcattc
     1801 cactgctatt agttgcgcta tttcgaatca cggcgttttt gaatttcacc tttgaaccag
     1861 ttagtttatt cgtatcagat gttgtaccaa taaattctgt tcgtttgact gataatttcc
     1921 aaccggcata atttccgctt ttatccgtta cttgtacgaa attctgtact tgtgtttgtg
     1981 ctccagtact gtccgttccg ctttgtaatg cagcatatac tgtttttgct gtacctaact
     2041 tgattgttcc aaaatcaaga tctggagctt ggtcaatcgt cagtaatccg gatgttccct
     2101 gatttggttc gtcaggtgta aaaggagtat caggatcatt tggatcaaca ggaggaactg
     2161 gaacaacgtc ttcaggattt gcagcatctg gtttggtcac agaaatcgtt ccttctgtag
     2221 tggctgagct cttattctca gcaaaaacac tttgtgtaca taataataga cttccagcta
     2281 gaacggtact taatgcaatt gtttttttca tttctttttc cttctttctt ttaaaataaa
     2341 taatccagca aataagccaa taagaatgac tccaacaagt atcagccatc gccagtcaaa
     2401 tgtttctggc tgatttttta aatcaatatc cttttcattc aattggtccg ctgcatcatc
     2461 agttatttca aaatcggtat ggagattcca gcgtttttct ttccaagcac ccttcacaac
     2521 cgcttgataa tgtcccggag ctaatttttt tccttctaat gggacaagta aatcaaaatt
     2581 tgtattgggt gctacatcta ttttttcttt ttttattgaa aggtaaggct cttttgtttc
     2641 ttttttgtag atttcaaccg atgtagtcaa gccgaacata taagaagcat ttggattttg
     2701 aagattcgcc ttaatcacat ttcttccatc gctttgatcc gcataagctt tttttaattt
     2761 caaatctggg gaagactctt tttcgctatt tcaaatcaag attgctttca cttgagaaaa
     2821 ctgattgacg acaccaactg tacttttgtt agaaacgttc tctttttttt cttgaaacgt
     2881 gatccctcct gcgaccacac cagccagctc tttttctggc gctttcagtg aaagtgtctc
     2941 agtgaccgca ctttgaggag gaacagttat ttctcccact gtttttgcta agtttttcat
     3001 atcataactt aatgtttcat caaatttact ttttgattct ccatattcga tcacaccgtt
     3061 tgaattcgtc gtagcggagt ggatcaatgt ctcaactgtt actgatttat ctgttcggtt
     3121 aaagaccgtg taggggattt cctgtgtttt cccaggctcc atccttacat ttatatatgt
     3181 tttgttctta tcaatctgat tagccggtaa ttcaaaatct accgcaaaat tgaattcgga
     3241 ggcatggaca ggtgtctgcc aaaggatgaa tccaaggaga aggatcataa gtaaagagca
     3301 gttgtttcga tgacttttat tccatattcg attcatcaat catcctcctt gatctttatt
     3361 ctgtcggcgc atctgctaat gtctatgtta tctctgctga gtaattacca gctatctgat
     3421 tttctttggt taagacaagt ttaaacgccc gaccgttatt taaatattct gaataatctg
     3481 cttgcacatt cgttgaattt tgtcctctga caatttccag ttcttcttca ttcactaatt
     3541 gatactcctc tcctttcttg aaatatagga catcctgcat agaaaatcct gcatcatttg
     3601 tgaaaggctg agataatttt atcgttaaga tccacggatt tttcgtttct cgctgatcat
     3661 aaatccataa tttttcctgt atatccattt ggccaaatgc ttcttgtgtc aaaccagttg
     3721 ctttgatctg accaaaatcg agtttggaag gggcaagaaa tgagagacca ccttctacta
     3781 cgatcttcgt agcaggtgca aaaggcgggg catcatgata aacaactggc gtatcacttg
     3841 gattgtgatt cccaatttca tcgttggtac ttggcttgtt ctgcacaccc ttagcagtag
     3901 cttctcctgc ttggtcgttt aatacaattt ctaatacatc tcctgcaaaa agttgatttt
     3961 ctgggataga aagaacaaat tcccctgtat catcaatagt tgtttctgac aaaacagctt
     4021 gatttttttt caatgaaacc aaggctcctg cttcacctgt acctgtgatc gtgcgagaag
     4081 ctgcattgat caatgtggta cttaaattag caggttcagg aggaatcaca tcgactgtcg
     4141 ttttggcaga gtccagttct gtcacttctt ggccacctct tactttttta gcagcaacaa
     4201 cttgcacttg atcctccgtt tttaatctct catcattggg tacttgaact tcgaacaagc
     4261 cgccttgttc gccttctccc catgggttat atcctgcccc ttcattttca tctgtttgtc
     4321 cttgcgtgag agcagtaatt tctacagatg atttcgtcgt atctgcgtaa gtaatacgta
     4381 aagtcacttc tacttcgcct gtccaagcat ctcttggctt tgcctcgaca ccttcgggaa
     4441 caacgacatg cccatagatt tttttatctg cattcgttgg cactcttaag gattcaatca
     4501 tcgggagctg attattggca ctgattcgat tataattttt cattccggca cctgtctgag
     4561 caaattcatt tgttaaacct gcactagggc ttgatacgaa attgcctaag tcggtcccat
     4621 tgatcgataa gtctgtcata tcagaaaaat aatccgggtc tttttcaaag ttatcgaatg
     4681 ttgcgcctgt ttttcgccat aaagcgattt ctgaatgggc gatctttaaa gaagaatctc
     4741 catttccttg gaatacccag ccgcctatgc cattggtctc gcttccaatc cgtttgtttt
     4801 ggaaatcaaa ataggtagga gattctaagt ccacattcaa cacatcccca ctgatgatgc
     4861 cggtcttatc ttcccctgtt ttcccgacca tgactaaata agttttttca cctgccgtaa
     4921 aatttaaatc aatatctcca gttgtatcta cagttgctcc ggaatctgcg cgtatatcga
     4981 tatttgattt ttctccagtg actgtaaaat cagctgaacc tggactttct agaaaaccag
     5041 aagcgtcata aaattggatc gcttgatttc cttgattgag tctgttcacg taagatttgc
     5101 catcacctgt atttcttacc ctcaatgagc ctccattggt tactgtcagc ttttgcgtac
     5161 cattctcaaa acgaactgcg ctggaacgcc cactctgctt atcgatgtcg ataactgatc
     5221 cggctccttc tacgatcaaa gaaaggcctt ttcctacaaa acgcatccca ttgttcccgg
     5281 gttgatcgtc atctccagcc attttgaccg agatttgtcc accacttgat acatggattt
     5341 gactagaatc tccttgcaag cgaaatccgc ccgtagttgc tgtattgatg gtgattttcc
     5401 caccattcga tacagtgact ttcgatcctg aacctctcat ttggatgatt ccgcgattgt
     5461 tgtcattggt aatattgatc ttt
//
LOCUS       DAZUMK010000150         5392 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000150 DAZUMK010000000
VERSION     DAZUMK010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.154"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            194..1303
                     /gene="pdhA"
                     /locus_tag="ACG808_002607"
     CDS             194..1303
                     /gene="pdhA"
                     /locus_tag="ACG808_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFS5048194.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            1306..2283
                     /locus_tag="ACG808_002608"
     CDS             1306..2283
                     /locus_tag="ACG808_002608"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFS5048195.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            2308..3951
                     /locus_tag="ACG808_002609"
     CDS             2308..3951
                     /locus_tag="ACG808_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFS5048196.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            3959..5365
                     /gene="lpdA"
                     /locus_tag="ACG808_002610"
     CDS             3959..5365
                     /gene="lpdA"
                     /locus_tag="ACG808_002610"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFS5048197.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
BASE COUNT         1789 a         1021 c         1237 g         1345 t
ORIGIN      
        1 ttcactaatg ctgttaatat gcggattggc ataatttcct ttcatcgtga aaatttgtgc
       61 ttttagcttg tatttctcat aaatccatga taatttatta atgaagacgg ttaaatagtg
      121 atacagtttt attcactgtt tcaatcgcag aatgaagaga ttttcttata gatgagagag
      181 gaatgtgtga caaatggcaa acaaaaaagc accgatcgac ttccaagcac tgctagatga
      241 agtcaatgca gatttcccag tatatcaagc acttgatcaa gatggaaacg tagttaacaa
      301 agatttagta cctgatttat ccgatgatga actcgttgaa ttaatgactc atatggtatg
      361 gtctcgtgta ttagaccaac gttcaacagc gctgaaccgc caaggacgcc taggcttttt
      421 tgcaccaact gctggacaag aggcaagtca gctggctagt gcttacgcat ttgataaaga
      481 agatgttctt cttccaggtt atcgggatgt acctcaacta gtaaaacatg gtttaccttt
      541 atctcaagct tttttatggt ctcgaggaca tgctgcaggt aattcttatc cggaagaatt
      601 gaaagctcta ccaccacaaa ttatcatcgg tgcacaatat gtacaagcag ccggtgttgc
      661 attaggtttg aagaaacgaa acaagaaaaa tgtcgtcttc acttatactg gtgatggcgg
      721 ttcatcacaa ggtgattttt acgaagcaat caatttcgct ggcgcttacc atgcaaatgc
      781 cgtattttat atccaaaaca acgggtatgc gatctctaca cctcgtgaaa aacaaactgc
      841 agcaaaaaca cttgcacaaa aagctgtcgc agctgggatt cctggtattc aagtcgatgg
      901 aatggatccg cttgcagtat atactgtatc taaaatggca cgggaatggg cgatcagtgg
      961 aaatggacca gtcctaatcg aaacactgac ttatcgttat ggaccacata cactatctgg
     1021 tgacgatcca acccgctacc gtacaaaaga cacagatgac gattggcaga aaaaagatcc
     1081 attgatccgc ttccgtaaat atttgacgga aaaaggactt tggtctgaag aaaaagaaga
     1141 acaagtcatc gaagcaacta aagaagaaat caaagcagct attgctgaag ccgacaaagt
     1201 accaaaacaa aaggtttcag atttcttgaa aaacatgttc gaagtatctc cacaatcaat
     1261 caaagaacaa attgcaattt atgaagcgaa ggagtcgaaa taatcatggc acaaaaaaca
     1321 atgatccaag caatcacaga tgccttagca cttgaactag aaaatgacga gaatgtcgtt
     1381 gtttttggtg aagatgttgg gaaaaacgga ggagttttcc gggcaactga aggattgcag
     1441 gaaaagttcg gagaagaccg agtattcgat actcctttag ctgaatctgg aatcgcagga
     1501 ttgagtttcg gtcttgcgtt agaaggattt cgtccagttc ctgaaatcca attctttggt
     1561 tttattttcg aagcaatgga cgaagtagta gctcaaatgg ctcgtacaag atatcgaatg
     1621 agcggcacta gaaatctacc catcacaatt cgttcgccat ttggtggcgg tgttcataca
     1681 ccagaattgc attcggataa tttagaaggt ttgatcgctc agtctcctgg tattcgagta
     1741 gtgattcctt ccaaccctta tgatgcaaaa ggactattga tttcttctat tagaagtaac
     1801 gatccggttg ttttcctaga acacatgaaa ctatatcgct cattccgaga agaagtgccg
     1861 gatgaagctt acgaagttcc tttggataaa gcagcagtta ctcgtgaggg aacagatgta
     1921 tctattatca catacggagc aatggtgcgt gaagcaatca aagcagcaga caatcttgca
     1981 aaagaaaata tatctgttga aatcattgat cttcgtacag ttgcgccatt agatgtagaa
     2041 acgatcattc aatccgtgga aaaaactggt cgcgtagttg ttgtccaaga agcacaacga
     2101 caagcgggcg ttgccgcgca agtagtttct gagatctcag aacgcgctat cctttcatta
     2161 gaagccccaa tcggacgagt ttctgcacct gatacagtct tcccatttgg acaagcagaa
     2221 aatgtctggt tgccaaatgc aaaagatatc gaagacaaag taaaagaaat cgcggaattt
     2281 taagaaaaga agggacgaaa caaagtcatg gcttatcaat ttaaattgcc ggatatcggt
     2341 gaaggaatcg cagaaggtga aatcgtcaaa tggttcgtca aaccaggaga tacgatcaat
     2401 gaagatgata cattactaga agttcaaaat gacaaatcag tagaagaaat cccgtcacct
     2461 gttacaggaa cggttaaaaa tgtgattgtt ccagaaggta ccgtagcaaa tgttggggat
     2521 gtattagtag aaatcgatgc accaggacac gaagacaatg aaggggacag tggtgtagcc
     2581 gcagaatccc aaactccagc taaaccggct gctgaaccca ctgttgatac agaatcagct
     2641 ggatcgtcaa gcgaaggagt cttccaattt aagttaccgg atatcggtga aggaatcgca
     2701 gaaggtgaaa tcgtcaaatg gttcgtcaaa ccaggagata cgatcaatga agatgataca
     2761 ttactagaag ttcaaaatga caaatcagta gaagaaattc cgtcacctgt cacagggaca
     2821 gttaaaaacg tgattgttcc agaaggcact gtagcaaatg ttggggatgt attagtagaa
     2881 atcgatgctc ctggacacaa tagtgcaccg tcaactagtg cgccatcagc tgaagcacca
     2941 aaagaaaaag tggaaacatc aggttcagca agtgtcgttg aagctgcaga tccaaataaa
     3001 cgtgtattgg ctatgccatc tgttcgtcaa tttgcccgtg aaaaagatgt agatatctca
     3061 caagtaactg caactggaaa aggtggcaga gtaacaaaag aggacatcga aaacttcttg
     3121 gcaggtggtc caagcagtgc accagcgaaa tcagaagcgc cagaagcggc agcaccgaaa
     3181 gaagcagctc ctgcagcaga aagcaaacca gctgcaccag caaaaccatt caagtccaac
     3241 ttaggagact tggaagaacg tgtagcaatg acgccaacac gtaaagctat cgctaaagca
     3301 atggtcaaca gtaagcatac agctccacac gtgacattgc atgatgaagt agaagtgtct
     3361 aaactatggg ataatcggaa acgatttaaa gaagttgctg ctgcaaatgg tacgaaactt
     3421 actttcttgc catatgtcgt gaaagcttta acagcgactg ttaagaaata cccagtattg
     3481 aatgcatcta tcgacgatgc aaaccaagaa atcgtttata aacattacta caatatcggt
     3541 attgcaactg atacggatca cggactatat gtaccaaatg tgaaagatgc agaccgcaaa
     3601 ggaatgtttg ctattgcaga cgaaatcaat gagaaagcaa aattagctca tgacggcaaa
     3661 ctatctgcag aagatatgcg caatggaaca attacgatca gcaatatcgg atctgtaggt
     3721 ggcggttggt ttacaccagt catcaactat ccagaagtag ctattttagg tgtaggaacg
     3781 attgcacaac aaccaatcgt aaatgctgaa ggcgaaatcg ttgtcggtcg tgtgatgaaa
     3841 ctgtcattaa gtttcgatca ccgtatcgta gatggagcaa ctgcgcaaca agcaatgaat
     3901 aatatcaaac gtcttttagc tgatccagag ctattaatga tggaaggatg ataaacaaat
     3961 ggtagttgga gattttgctg tagaattaga tacagttgta attggtgcgg gacctggtgg
     4021 ctatgtagct gccatccgcg ctgctgaaat gggacaaaaa gtagcaatca ttgaaagaga
     4081 gtatattggc ggtgtttgtt tgaatgtcgg ctgtattcct tcaaaagcac tgatagctgc
     4141 aggacaccat tatcaagagt cactagattc tgaaatgttt ggagtaacta gtgaaaacgt
     4201 caaactagat tttgcaaaaa cacaagaatg gaaagaaaac aaagttgtcc atacgttgac
     4261 ttctggtgtt ggtttcctat taaaaaaaca taaggttgaa acaatcgaag gagaagcgtt
     4321 cttcgtagat gatcacacac tacgcgtcat tcatccagat tcagctcaaa cttattcgtt
     4381 caataacgca atcatcgcaa caggttcccg tccaatcgag attccaggat ttaaattcgg
     4441 aggacgtgtg ttagattcta ctggcggatt agcattgaaa gaagtaccta agaaatttgt
     4501 gatcatcggt ggcggtgtga ttggtgccga attgggcgga gcgtacgcaa acttaggagc
     4561 agaagtaacg attcttgaag gctcaccaca aattttacca acttacgaaa aagatctagt
     4621 aaaattagta gaagatgatt tcaagaaaaa aggcgtaaca gtcgtaacga atgcaatggc
     4681 gaaagaagca gtcgataacg gtgacagcgt gacagtgaaa tatgcagtgg acgggaaaga
     4741 agaatccgtt actgctgatt atgtgatggt cactgtaggt cgtcgtccga atacagatga
     4801 tatgggctta gaacaagcgg gtgtcgaagt aggagaacga ggattgatca cggtggataa
     4861 acaaggacgt acgaacgtac ctaacatcta tgccatcggt gatatcgttc caggggcggc
     4921 attagcacac aaagccagtt atgaagcaaa aatcgctgct gaagcaatct ctggcaaaaa
     4981 agtagctgta gattataaag caatgccggc agtagccttc actgatcctg aattggcttc
     5041 tgtaggtatg acgatcaaag aagcaaaaga tgctggtatc gaagcaaaag catataaatt
     5101 cccattttca gggaacggtc gggcattatc gcttggtaaa acagaaggat tcatccgtct
     5161 agttacgacg atcgaagata acgtgcttat tggcgcgcaa atcggtggag tcggcgcaag
     5221 tgatatggtc tctgaattag ctttagcaat cgaatcagga atgaatgctg aagatatcgc
     5281 cttgacgatt catcctcatc catctcttgg cgaaatcgtc atggatgctt cggaattagc
     5341 attaggttta ccaatccata tttgatttga ataatacagg cgaaacgcaa ct
//
LOCUS       DAZUMK010000151         5341 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000151 DAZUMK010000000
VERSION     DAZUMK010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.155"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..546
                     /locus_tag="ACG808_002611"
     CDS             <1..546
                     /locus_tag="ACG808_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS5048198.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSPVQFRKTTVTVV"
     gene            complement(716..1678)
                     /locus_tag="ACG808_002612"
     CDS             complement(716..1678)
                     /locus_tag="ACG808_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5048199.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1680..3119)
                     /locus_tag="ACG808_002613"
     CDS             complement(1680..3119)
                     /locus_tag="ACG808_002613"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFS5048200.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            3304..5250
                     /locus_tag="ACG808_002614"
     CDS             3304..5250
                     /locus_tag="ACG808_002614"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFS5048201.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1720 a          914 c          889 g         1818 t
ORIGIN      
        1 atccatcgtc gtttctacac cagtatctgc catcagaaaa tcacgacgga tacaactgaa
       61 tttaaatacc atgaagtgga tgccaaaggc attatccgtc aaaagaaatt gtatctagat
      121 ccttttatgg atatgttcaa tagtgagatt ttatcctatc gtatctctga aaagcccaat
      181 gcattagcgg taatggaagg tttggaggaa gctatccaga ccactaacga ttgtccctat
      241 cgtcgtacgt ttcactcaga tcaaggttgg gcgtatcaaa tgaaagctta tagaaacaaa
      301 ttgaaggagt acaaaatctt tcaaagcatg tctcggaaag ggaactgctt agataattca
      361 cttatggaga atttttttgg cttattaaaa caagagattt ttcacgggaa agtttataac
      421 tgtttcgagg aattaaaatc agccatcgat agctatatct attactacaa taatgaacgg
      481 ataaaacaga aattaaactg gcaaagtccc gtccaatttc gtaaaacaac cgtaacagtt
      541 gtgtaaaaat atcattttag agcatgaaaa aaggagtgga tttctccact ccaaaaaagt
      601 ctaacttttg ggggtcacta caagttgaca actccttttt gtttacaaat caattggata
      661 tctacaaatt gtattttcta acgaatttta tagacattaa aaaattaatt taacgttaaa
      721 tacttcctcc agaaagtatt gagattggta aaatataatc cttatttgtc ttttcgcctt
      781 taattttttt cagaagaata tcaacaataa gacttgcaat ttcatcaatt ggttgtctta
      841 ttgttgtcaa ttgtgggaaa aatttctcaa taaaagaggt accatcgtat ccaataattt
      901 ttaaatcttc tgggatatta agtttaagtt gatgagcaat tttcatagtg agaatggctg
      961 ttaaatcatc agaaacaaaa ataccatctg gtttgtgatt tataatagtt gactttattt
     1021 ccatttcacg acggatagta gacagattat taggaatttt gaaaattttt ccatttggat
     1081 tttgaaaaga aaaacctagt gcacgtaatt ctgtgggaga atccgtgtta tcatttcctg
     1141 taatcataat ggtattttga caaccatttt tttgtaaagt cttagctgca agttttcccc
     1201 cttcaaagtt atctgaagaa acaatggaaa cattaggagc taaatttcta tcaaaagcaa
     1261 caataggtgc tctcacacgc tcataatcag caattccaag attatggctt gatgagatta
     1321 tgccatcaac ttggttggct tcaagcattt caatgtattc tctctcttta gcaggatcat
     1381 tttgactatt gcaaataatc gttttatatc catgtttaaa taactctatt tcgagatact
     1441 caatgagttc agagtaaaag atattactaa tattaggaaa aataagacca ataagctgag
     1501 gtgactttcc ttgaagtcca cgtgctaaat tatttggttt ataaccaaga gttttcatag
     1561 cttcatgaac tttttgtttt gttttttctg ataaatatcc cttattatta atgacgcgtg
     1621 aaacggttgt tgggctaact ccagctaatt cagcgacatc agttaatttt gctaccattt
     1681 taatatttta attcaaagta ttttccagta ggttcgcctg atttgatttg aataccattt
     1741 tggtgatttt cagggaatac acgactagta aacacttttt ctcctttatt aataaaaatc
     1801 tcaatgattg agttatctac aaaaatgtta gcagttgttt ctttagtatc aattgagcat
     1861 tcacgaatcg taccaaagtc agtggcatat tgaatgcctg ctttgctgcg atctaaaata
     1921 atttttcctt ctttggtatc tacaatcaag ctaagtccat ttcctttact atcagagaaa
     1981 agaagtaact ccgatttctg atttggagga aaagttagtt caagctcata agtattattt
     2041 gtttctgctt ttgctgtaaa ttgttcagaa ctagcacgta atgatgtaat agcttctact
     2101 ggatattggt aaagtttacc atcttttatt gaaagttctt ttacaagact cattgctccc
     2161 tgataatcat acttatcagt tggataatcg atatcaggta gtccaatcca actgacaatc
     2221 agagcacgac catcaggagt attgaatcct tgagtcgcat acgcttcaaa tccataatca
     2281 agattttgaa tttctgaagc gcccacaagt tttacttttt ctatgtcaag cgattgacag
     2341 atcttaaaag tattcggata gatattccca taatttagct cagatttatc caacccttga
     2401 ggacaataaa tgagaacagg ttttttgtct acaaatacta gatttggaca ctcaatcatg
     2461 tattctgaac cagtgccgcc aaaatcaagg ttgccaactt cctcccatgt ttccacgtta
     2521 ttgtcatccg ctttgtaaag tttgataaag cctgattttt ctaaattttg tgctccaaca
     2581 atcgcataca attgtccttc ataattaaac atttgtggat cacgaaaatg atcagttaca
     2641 tcagcaggtt tatgaataag aacattctca cctttcgtaa tgttgtagtc tttatccatc
     2701 catgctccga tttgaagtgt atcacgttcc caattttcat ttcgaacgtt tcctgtatag
     2761 agtaagaata gattcccctc tatttcatat gcagaaccgg aataagcgcc gtggctatct
     2821 cgttttgtgt ctggaagaag ttctgctcct gttacatgaa aatgaactaa atcatctgat
     2881 tcattgtgta cccattcttt caacccatga gcagctccaa atggccaatt ttgataaaaa
     2941 agcgtatatt ttccgttaaa aaaagaaaaa ccattaggat cgtttaacaa tcctgtttta
     3001 ggttcgatat gatagctagc acgccaaggt gatcgtttta cattatcctt aattttatcc
     3061 atctcatctt tagtccaatc agaataagca cgatatcgta tttctcgagg taagttcata
     3121 ttttactcct tcaatttatc tttttacaac tagtatggta aacgttttcc gttgaaaaat
     3181 caatcatttc atttaaaaaa ataaactata tgacaaacgt ttgacatatt tttaagatat
     3241 gctaaaataa atttgcattt cagtgaaccg ttttcacgta aaaataaaaa aggagtgatt
     3301 cgaatgaatt acagtaaagt agcttccgac gtaatacaag cggtcggaaa agataacctt
     3361 attgcagctg cacactgcgc tacacgtctt cgtctcgtac taaaagatga cacgaaagtg
     3421 aatcaaaaag ctttagacga aaatccagat gtgaaaggca catttaaaat tgatggacaa
     3481 tatcaagtga ttatcggtgc aggtgatgtt aatttcgttt atgacgaact tatcaaaaaa
     3541 actggacttt cggaaatttc aacagatgat ttaaaacaaa ttgctagtaa agataaaaaa
     3601 ttcaacccca ttatggcatt aattaaatta ttatcagata tttttgttcc aattatacct
     3661 gcccttgttg caggtggttt attaatggca ttacaaaact ttttaacagc tgctggactt
     3721 tttggaccaa aatcaattga agaaatgtat cctgcgattg aaggtatttc ctcaatgatc
     3781 caactgatgt ccgctgcacc attcatgttc ttaccaattt tagtaggtat ttcagctgct
     3841 aaacgttttg gagcaaacca atttcttggt gctgcaattg gtatgatcat gacaacacca
     3901 gatcttggtg gctcaactga atattggaat atttttggtt atcatgtttc acaaacaaac
     3961 tatgcttatc aagttattcc agttttagca gctgtttgga tattatctat attagaaaaa
     4021 ttctttcata aaaaattgcc atcatctatt gactttacat tcacgccatt attatcagta
     4081 atgattactg gatttttgac tttcacagtt attggccctg taatgttatt gttgtctaat
     4141 ggaattacag acattattgt ttggctatat aacactactg gatttattgg aatgggtatt
     4201 tttggaggaa catattcact tatagtcatg actggtttac atcaatcatt tccagctatt
     4261 gaaactcaat tactttcagc gtggacaaat ggtatcggac atggagactt tatctttgta
     4321 gttgcatcta tggcaaacgt tgctcaaggt gctgcaacat ttgccatttg gttcttaact
     4381 aaaaattcta agacaaaaag tcttgcttcc tctgcgggtg tctcagcact tcttggaatt
     4441 acagagcctg ccttgttcgg agttaactta aaataccgct ttccattctt ttgtgcttta
     4501 attggttcag gaattgctac agcagtagca ggattactta aagtagttgc tgtttcactt
     4561 ggttcagcag gtttcttggg atttctttca attaatgcaa catctattcc attttacgta
     4621 ttgtgtgaat tgattagctt tgtagttgct tttgcaatta cttacttcta cggtaaaaca
     4681 aaagctgctg gtatctttaa agcagaagaa attgctgaac aaacaccagt taattcttca
     4741 aaagtagcaa caacagatcc aatatctact gatgttgaat caacacaaac aaaagtttca
     4801 tcagaaacaa ttgttagtcc catttctggt aaagtgatta gtctttcttc agtaaatgat
     4861 ccagtctttt catcagaagc tatgggaaaa gggattgcca ttaaaccaaa tggaaacacg
     4921 gtccattctc cagtagatgg tactattcaa ctcacgtttg aaactggaca tgcttatggt
     4981 attaaatcaa ataatggtgc tgaagttcta attcatattg gtattgatac tgtatcaatg
     5041 ggaggaaaag gatttacaca aaaagttact actaatcaaa aagttagaaa aggcgatatt
     5101 cttggagcat ttgaccgttc aatgattgag aaagaaggtc ttgatgatac aacaatgatt
     5161 attgtgacca atacttcgga ttattcagaa gtaaatatag tagctgaaaa tacagtcact
     5221 gagggtactg atttacttga aatcaaataa atatatttcc ctgagtaaag atggaatttt
     5281 ctttccaaaa tcaaataata tagattaatc aaatatttga gaataacatt ttatttaatc
     5341 t
//
LOCUS       DAZUMK010000152         5238 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000152 DAZUMK010000000
VERSION     DAZUMK010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.156"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..360
                     /locus_tag="ACG808_002615"
     CDS             4..360
                     /locus_tag="ACG808_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-rich SFCGS family protein"
                     /protein_id="HFS5048202.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
     gene            376..726
                     /locus_tag="ACG808_002616"
     CDS             376..726
                     /locus_tag="ACG808_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HFS5048203.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            755..1534
                     /locus_tag="ACG808_002617"
     CDS             755..1534
                     /locus_tag="ACG808_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HFS5048204.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            1545..2222
                     /locus_tag="ACG808_002618"
     CDS             1545..2222
                     /locus_tag="ACG808_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HFS5048205.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAMILG
                     AIFPAVN"
     gene            2241..3347
                     /locus_tag="ACG808_002619"
     CDS             2241..3347
                     /locus_tag="ACG808_002619"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HFS5048206.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            3337..4428
                     /locus_tag="ACG808_002620"
     CDS             3337..4428
                     /locus_tag="ACG808_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HFS5048207.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            4441..5181
                     /locus_tag="ACG808_002621"
     CDS             4441..5181
                     /locus_tag="ACG808_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HFS5048208.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
BASE COUNT         1675 a          862 c         1251 g         1450 t
ORIGIN      
        1 atcatgatta aagtagttat tgcagaccgt atgggtaaag gacaaaatgt ggccaaaggg
       61 gtagaagcag ctggtggaaa agcagttgtc gtaccaggaa tgggggcaga tatgcgtttg
      121 ggcgatgtga tgcaacaaga gcatgcagat atgggtatct ctttttgtgg tagtggtggt
      181 gcaggtgcat taacagcagc aacaaaatat ggctatccag aacgacatgg tatgcgttcg
      241 attgaagaag ggatcacagc tatcaaggat ggcaaaactg ttctaggttt cggctttatg
      301 gatcaagagg aattagggaa acgtctgaca gaagaattta tcaaaaagca tggaaaatag
      361 aaagagggac atgaaatgag cgttcaaaag acaattcagc aaaaagttat cgtctccggt
      421 aaaggggaaa caaagcaaca tgcttttgca tcagcgttag cagaaattca aaacaaaata
      481 atggaacaat caaacgtatt attgcgaatc gaaccagtca atgtggatat tttaaaggcg
      541 gtcgaaaaca aatacacaga acgctttcta tttttcttct tcccacggca acgtattagc
      601 tatgaagtcg aactctctgt tactgtggct gtaacagaaa taaaattggc agacatatcg
      661 ttcagccagc aaatgactga agatccaaat gggattgcca ttccatttgt aactaaaaaa
      721 atctaatttt gaatcataag ggggaaagaa taacgtggat ttcgtaatca ttctagtcaa
      781 atcggtcatt atcgggggat tacttggttt tgcagcatct gtcggggcag ctcgaatgtt
      841 tcatgcacct aatacacaag gtttaggagc ttttagaaca ttaggcgaaa tgaatgcctg
      901 catgggtgat ccagcttctc atttttcatt tggtttaggc tttttcttta atgcatgggc
      961 ttcttcagtt ggtgctggtg cattcacaca agatgtgacg catcgaatca ttcccaattg
     1021 ggcagcggca gcacttctag caaaaaacaa agatgtcacc cagaccatgc atgatccgaa
     1081 aaaaatgggg atcattggag cggttatcgg ggcagtggtg gtagccttct taaacacaac
     1141 agctgcagca attccagagg cgttacaagt gacggcggtt tccgttttgg taccagcagc
     1201 gacgattctg atcaatacag taatgccagt tattttttgg ttggcagctt tagacgcagg
     1261 gaaacgaact ggtttttggg gcactctttt tggtggtatc gcgcaattga ttatgggaaa
     1321 tgcggtacct ggtgttgttc taggaatctt gattggtaaa ggcgtcgatg agagcggttg
     1381 gaatcgcgtc acgaagagca tgatgatcgc tattgtttta ttatttattt tcagtggatt
     1441 tttccgtgga tttgatatgg aaatgatcaa gagcttccag cttcaagtgc ctaattggct
     1501 tgataacttg cacaacatct ttagtgtgaa ataggaggag aatcatggag acaacagaag
     1561 aacaagcaaa agtattagaa gcaaagcggc aaaagagctt ttggtttgca gactggtctt
     1621 ttccgctaat cgtaggaatt atgtccgctg ctgtatttgc aggaacacac atgtatgtga
     1681 tgtatggtgt gggtgcattc aatgaagttt cgatcgtggc gatgttaaaa gccgggctgg
     1741 atggcggatc ctatggcgca gcggcagcgt ttggtgctag tttcttattt gccagaattt
     1801 tagaaggctc tttagttggg atcttggact taggcggttc gatcctaact gggatcggaa
     1861 ttggggtacc agcaattctc ttaagtatga aaatagcggc accaatcgat aattttgcac
     1921 tctctttatt gactggtttg gttctaggtt tgattgttgg tggcatcatt gtcttgattc
     1981 gtaaatttac gatcaatcaa tcaaattcca cttttggtgc tgacgtcatg atgggggcag
     2041 gaaattcatc tggacgtttt ttgggtccat taattatatt aagcgcgtgt gcagcatcta
     2101 ttccaatcgg cattggcgct acattaggag cactaggatt ctatgcttgg aaaaaaccaa
     2161 ttgctggagg tgctatttta ggagcaatga ttttaggagc aatcttcccg gcagtaaatt
     2221 aatagaaaga gggtcattaa atgtttgata ctttgattaa aaatggaagg ttagttgatg
     2281 gatctaaaat agaggtcgct atagaaaaag gcatgattaa agcagttgca gcagaaataa
     2341 atcagccggc gcaagaagta gttgacttgg agggaaagta ttatttatcc gctgggtgga
     2401 tcgatgatca tgtccactgc tatgaaaaga tgaatcttta ttatgactat cctgatcaga
     2461 taggggtcga aaaaggtgtg acgacggtca ttgatgctgg aactacggga gcagaaaaca
     2521 tccgtgaatt ttatcaattg acgaaatcgg tcaagacaaa tgtgtatgcg ctaatgaata
     2581 tctctaaatg gggcattgtc aaacaagatg aacttgctga tctaacaaag attcaagaag
     2641 atctagtaag tactgcgtta gcagaactgc cggaatttat tgtcgggatt aaagcgagaa
     2701 tgagtaagac agttgttggt gaaaatggca ttaagccatt agaattagct aaaaaaatcc
     2761 agacagaaaa caatgatctg ccattgatgg ttcatatcgg ttctgcacct cctgagttag
     2821 aagaagtatt gtcaagaatg gaaagaggcg atgtattgac ccattgtttt aatggaaaac
     2881 caaacggtat tttagatcca aagacagatc aaattaaaaa ctttgtctgg gatgcttaca
     2941 ataatggtgt cgtctttgat attggacatg ggacggatag ctttaatttc catgtggcag
     3001 aaaccgcact aaaagcagga atgaaagcta cctctattag tacggatatc tacatccgca
     3061 atcgtacaaa tggcccagtt tttgatctag caactacttt agaaaaattg agggtagtcg
     3121 gctattcttg ggaagaaatc attgaaaaag ttacagcagt tcctgcacgg aattttcatt
     3181 tgaagaataa gggccagtta gctgtcaatt atgatgcaga tttgacgata tttgagattg
     3241 ttgaaaatga aaaggaattg aaagattcca acggatttac acgtatagct aaagaacaaa
     3301 ttttaccgat taaaacaatt attggaggcg tcatatatga caattagcta tggaaagttt
     3361 gatttaaaag aagtcattaa tgcatcggga aaaatgacga tcctaggggt ttctaaggta
     3421 tctgaaagtg tcctagcagc acaacgattt ggaggcgagc atttttttga gatgagtgat
     3481 cttgccatca aaaccggcgc ttatctttct caattactga aagtggaaga tgcgcaaatc
     3541 gtttcttctg cttcagcagg gattgctcaa tctgttgctg ctttaatcgg acagggcgac
     3601 atgtaccatg tctatcatcc ctatacagaa aaaatcgcta gacgggaaat catcatacct
     3661 aagggacata atgtagatta tgggacaccg gttgaagtaa tggttgctca aggtggagga
     3721 aaagtcatag aagctggcta tgcaaatatg tgtacaccgg aacatgtttc catgatgatt
     3781 acggaaaaaa ctgctgcgct tctatatatt aaaagtcatc atgcagttca gaaaagtatg
     3841 ctgacggtgg cagaaatggt agaagttgcc aaagctcatc gtttgccatt gatcgttgat
     3901 gcagctgcgg aagaagattt attcaaatat agcgaaatgg gcgttgactt agtgatttac
     3961 tctggggcaa aagcaatcga aggtccgagt gccggactgg tcataggaaa aaagaagtat
     4021 attcaatgga ttcggctaca gtctaaaggt attggccgag caatgaaaat cggtaaagat
     4081 aatattttag gctttaccca ggcactagaa gattatttaa aaaatgggag cgaatccggt
     4141 gactccatga agcaacgatt agcgcctttt gttaacgcaa tcaatcaact tcctgacctt
     4201 tcagcgaaaa tagttcaaga tggtgccggc agagatattt atcgagcaag tgtaacgatt
     4261 aatggcgata aaagtgcgaa agaagtcatt caggaattaa aagcacgaaa tccagcagtc
     4321 tacacccgag aatatcaagc aaataacgga atcattgagt tcgacattcg ttccgtcagc
     4381 caaaacgaaa tggaaaaaat tataaaaaga ttgagagaaa tcatgtagga ggcttttaaa
     4441 ttgtcagtta tcccaaatta ttataaaaat cgtgtatgtt taaatgtatt agctcattca
     4501 gttgaaaatg caaaagcatg ctatgaagca gcggaagggc atgtcgtact aggtgtgcta
     4561 tcgaaaaatt atgctagcga tgaagcagca atcgaagata tgaaaaggta ccaagacgca
     4621 accaataatg cgttgtcagt tggattaggc gctggagatc caaaccaaag tcagatggtt
     4681 tcgcgtttat ctggggtttt acaaccacaa catgtcaatc aagtatttac tggcgtgggg
     4741 acttctcgtg ctttattggg ccaaaatgat acagtcatca atggattggt ttcaccaaca
     4801 ggtaagatcg gctatgtcaa tattgctaca ggtccgttaa gttcacaagc gccagcagct
     4861 gaagtgccta ttgaaactgc cattcaaatg ctgcaagaca tgggaggtac ttcaatcaag
     4921 tatttcccga tgaaaggctt agcacatagg gaagaattta aagcagttgc ggaagcttgc
     4981 gcaaaatatg aattctattt agagccaaca ggaggaattg atctttcaaa ctttgaagaa
     5041 attctgaaaa ttgcaatcga tgcaggtgta aaaaaagtga ttcctcatgt ttatagttca
     5101 attatagata aagaaacagg agatacgcgt ataggagacg taaagatact attaagcatg
     5161 gtaaaaaaaa ttattcacta actcattaga aaataagaag cttattccag aatgaaatta
     5221 aatggaataa gcttttta
//
LOCUS       DAZUMK010000153         5212 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000153 DAZUMK010000000
VERSION     DAZUMK010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.157"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            454..954
                     /locus_tag="ACG808_002622"
     CDS             454..954
                     /locus_tag="ACG808_002622"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048209.1"
                     /translation="MNYNKNLLSHIVYFNYIIIGVYHLNLTQRRGYFETQKENLSRSP
                     LFLDDWNSQIESKSLNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTA
                     GYGNYSNSRHALALFGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWD
                     NTITNW"
     gene            927..1370
                     /locus_tag="ACG808_002623"
     CDS             927..1370
                     /locus_tag="ACG808_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048210.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2540..3508
                     /locus_tag="ACG808_002624"
     CDS             2540..3508
                     /locus_tag="ACG808_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS5048211.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            3525..4049
                     /locus_tag="ACG808_002625"
     CDS             3525..4049
                     /locus_tag="ACG808_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFS5048212.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTETHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKTEKKWWQFWQ"
     gene            4425..4706
                     /locus_tag="ACG808_002626"
     CDS             4425..4706
                     /locus_tag="ACG808_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048213.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            4708..5124
                     /locus_tag="ACG808_002627"
     CDS             4708..5124
                     /locus_tag="ACG808_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048214.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1967 a          893 c          868 g         1484 t
ORIGIN      
        1 aaaaaaaaga aaaatccgct tatgcaactt cttctttttg tattttgggg cgctacccca
       61 atccccaagt atcggttatt taatagcgcg gaaatccaaa gaataggacg taccctgaaa
      121 caaaaaaaga ctgctctcac gagcagccca aaatgagatt atcctattca tcaacataaa
      181 aatattacaa agtgaagaac tagagaaaga caaaaaaagc atatcaaatc ctctcaaaaa
      241 taacaagtct taaaaaaatg acagataaaa atgcacaaag aattagatat agataaccgc
      301 gaaaaatcaa gctttagaaa gttgctttta gctagaccaa ttaaaaatca caaagtgagc
      361 gtctctatcc attggtagag gggctacttg gcatagtgta cattatgtta actagctcga
      421 taaataacga ttatgttccc aaaacaatat tttatgaact ataacaaaaa tttgttatcg
      481 catattgtat attttaatta tataattata ggtgtatacc acttaaattt aacacaaagg
      541 cgtggttatt ttgaaaccca aaaagaaaat ttatctaggt ctcctctttt ccttgatgat
      601 tggaatagtc aaattgaatc aaaaagtctt aatcaagatc aaataataaa atatgcaaat
      661 tactgtggtg tttatcctaa aaaagtagca cgaatactaa acctttcaga ggtccaaaca
      721 caactaagaa aaggaaatgc catttatatg ggaactgctg gttatggaaa ttatagtaac
      781 agccgtcatg cacttgcatt atttgggtgg gtcaatcaag gtggttatca aacttactat
      841 gtgtggaatc cttggtataa ctacccaact gtaataagtg caaatgcagg accagttaca
      901 attcctgtac ctggaggtgg ttttacttgg gacaatacca ttacaaattg gtaaataaac
      961 ccattgcttt agttatgggt ctagtgttag ttcttttctt aactagttgt tcaaaaaaac
     1021 aagaaataca aaagactgat ctgattcctt ttctaggaac ttggcaaact aaagatcatt
     1081 cagaacgtct agtaatcact aatttaaata cttcaaaaaa tatagaacaa tataccatct
     1141 tagtaaaaga gaacaatcat aaaattcaaa tcattgataa aaatttaaat ataaatgaac
     1201 aagttttcat ttacaaagca acaaataatt tactaacact gcgctctaat aaaaaacaat
     1261 tagattacac ttttagtttg gattctccaa aaaagctcgt atttattttg acaacgaatg
     1321 atgggacaga agggacagct aaaccaattg aattttttaa agctgattga tgaatagttg
     1381 tttgatatta caaaacaata atttcaccga aggtgaaaca agcgaaactt tacaaaaagt
     1441 ggctagcatt tgctagcctt ttttgtattg tggagcgtgg tttttgaggg ggattcaagg
     1501 gggagcttgc gcccctttga caagcggtta tttgcaccaa atacgtaggt atttggctag
     1561 caaatataga gcttgccaaa cactttagtg agttttgtag aaagtacctt taaaggtact
     1621 ttcacattac gaacgatacg tccgtaggac gtactgaaag ggtgggattt atgagcgaac
     1681 taatgcaaaa tttggctagc aaaaaaagtt aaaaaatata tgctaataat cgagcgaaat
     1741 gaacactttt tcaagtgagc gaatccgcca tgacaaagct gaagcagttt atttcagctt
     1801 tgtcatggcg tgtgcctaga cttttaaaaa agtcggcaca cgagcaaata tctttatcaa
     1861 gctctaattt gcgttttaag acgtttgaaa cgtctaagta tataaatata taagagtagg
     1921 tttaagagtg cttaaatcga attttagagg gcattcagac aactttaggg ttgttagggc
     1981 ttgccctgac cgcctgtaag gcgctttaag aacaagataa gggatttata ctaagactgt
     2041 cttaaaacgt ctcaaacgaa ggctcatagc ttctcaaaga gttccttatg aacattttta
     2101 gcaaaggagc cggaagcgac tgcgcagtat aatgtgagag cagagcgatc acaccttttt
     2161 aagcgactgt agggagtaag attcccagtt gagaaaaatg cgcgaatctg tttggagaaa
     2221 aaagaagtgg ttttttaggc gggaaatttt gtccccctct tttataatct ctttttaaac
     2281 ctctttttaa acctctttac cgtcggctat atccttactc tcccaaggga atgacgcttt
     2341 tatctacatc gttttgtctg tttatctaca tcgttttgtc tgtttatcta catcgttttg
     2401 tctgtttatc tacatcgttt tgtctgtttg tacattgaat tagtacatcg acgatgttaa
     2461 tataagatgt agataaacag gcattttgta tttcataacg tgtatctctt gtgcgagtaa
     2521 gaaaggagct attttaaata tgtctaatga aatcgtaaag tatcatcatg aattaaatac
     2581 aattccgttg cgaaaattta caccagtcga aatgaatttg ttcttttcta tcgtatcaag
     2641 aatgcgtgat gtaggtgata aaaaagtaca atttacattc gagcaattaa aagatttaag
     2701 caattataaa gcaactgcaa atgttcgatt catagatgat ttagaaacaa cgtatgacaa
     2761 attaatggat ttacgttttg gtagaagaag tgctgacggt ttgcaacgtg aacgttttgt
     2821 tttatttaat caatttaaaa tcgacggaaa atctgatatt ccttttgctg aaatacaagt
     2881 tcatgaaaaa gccttaccct tacttaataa tcttgaagaa tgggtgcgct attctctcca
     2941 acaattcaat gaattagaaa gcagttattc aaaaacaatg tttcgattac taaaacaata
     3001 cagaaccaaa ggtttcgctt atttttcaaa agaagacttt catgaattac ttgatattcc
     3061 taaaagttac aaccaaggta cgattgatca acgtgtgttc aatccaatac gacaagaact
     3121 aaccgcaatc tttaaaggtt taacaatcaa aaagaaatac ggaaaaggac ggggaaagcc
     3181 tgtcattggc tatcaattta catttaaacc tgagataaaa aaatcagacg actttaacaa
     3241 acatgcccca acaaaagaag aacaaaacaa agcattagaa ctagctaacc aacaagttca
     3301 atctgaaaaa gcacctactt cattacaaga aaaaatcaaa gaacaaaagc gaaaattgga
     3361 acttttacaa cagttagaaa agttagaaag agaacaaaat acccaaaata aagaaaataa
     3421 tgcacaaaaa tcagagataa attccgatat tccttcagaa ctaaaaaaag acttattaga
     3481 caatcttcaa aacttattca aagattgagg ggtttttaga ttttatgaca gaaaaaacaa
     3541 tcaaagaact tgctgaagaa ttaggagtta gtaaaacagc aattaacaaa aaagtttctg
     3601 acgaaaatcg aaaactttgg ttttcgaaaa tcggaaacaa gtttgtgatt aatgaaaccg
     3661 gacaaaaggc tataaaatca atgtttttta acgaaaacga aaactcaaat caacaactag
     3721 ttggcgaaaa aacgaaaacc gaaacccaca aaaacgccaa ccaagttccc gaattagaag
     3781 tcctttctct cctagaacaa cagttaaatc aaaaagatat gcaactacaa gaaaaagacg
     3841 agcaaataaa acaaatgcaa aaattactcg accaccaaca acaactcacc ctgcaagcca
     3901 accaacaaat cgagaaactg caggaacaac tacagttgac ttatacggaa gaatcttcgg
     3961 aaaataaatc aacgactctt tctgaagaaa cagaatcgat agaaaaacaa tcaaagacgg
     4021 agaaaaaatg gtggcaattc tggcaataaa accagcaatt taaattaata aaaattgctg
     4081 gttttattct tctttttgct tttgcgagca gagcaacatg gacccatgtt gcgatatttt
     4141 ggtggcgccc caatccccgt tttttcgttg aatcttactc ggactgcact cgttttacag
     4201 ctgatttttc tgaattttga aagagcataa ggaaacgtgg tatcactttc ttacgaaagg
     4261 tgctagatag gctgtgccta tcacgtttct cagaaaaaaa tttgctatca cttttttgcc
     4321 attgtggcta ggctatgtat agtgttttgg cgaaaaatag ctatacaaat actatatttg
     4381 tatggtatac ataaattata cgctgatacg aaaggaaaat ttccatggac attttagttc
     4441 gaaatatcaa tcccaaaatt attgcaagaa tagataaacg ttgtcaggaa ctcacaacca
     4501 aaacgggcaa aaaatggagt cgaaataaat atctaaaagt tctaatcgaa agtgattttg
     4561 accgtcaact tatgaattac aaaaaagatc aattcgattt attactcgaa aaatttattg
     4621 ccattcaaac agaaaatacg aacatactga atgactatgt tcacacaaac aaccaactca
     4681 ttgaaacact tatcggagga atataacatg agaaaaactt ctgttcaaaa acacgtaacc
     4741 ctttatccca gtcacgttga cgttttagat tccatcatcg aaaaaaatcc agacgtcaac
     4801 aattattcag aagccattcg ttttttatgt atgagttatg aacctgatac caatacaacc
     4861 acaaccagta accaagcaaa actcaatgcc atgggaaaag aaatttctat catgacagaa
     4921 atcatggcag aatttgctga tttatatcta aaagatactg gtgttcttat cggtacaaat
     4981 tctaaagtgt atcaagaagt gaaagaaaaa attgaaaatc aactcaatcc ttcccgacga
     5041 aatcaaccca aaaatcgtca aaaagaaacc aatcaaacct ttacttctgg aaattttgac
     5101 ccaacaaact ttctaaaaag ataaaaaaag aaaccgatcc ctaaaaaaat tcctcgattt
     5161 ctttttaggg atcagtcttc cagcaagatt tgcaagttgg taccaacttg cg
//
LOCUS       DAZUMK010000154         5192 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000154 DAZUMK010000000
VERSION     DAZUMK010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.159"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(297..1271)
                     /gene="sgrA"
                     /locus_tag="ACG808_002628"
     CDS             complement(297..1271)
                     /gene="sgrA"
                     /locus_tag="ACG808_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFS5048215.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(1467..2306)
                     /locus_tag="ACG808_002629"
     CDS             complement(1467..2306)
                     /locus_tag="ACG808_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFS5048216.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            2491..3378
                     /locus_tag="ACG808_002630"
     CDS             2491..3378
                     /locus_tag="ACG808_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFS5048217.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(3972..4667)
                     /locus_tag="ACG808_002631"
     CDS             complement(3972..4667)
                     /locus_tag="ACG808_002631"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFS5048218.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(4651..5049)
                     /locus_tag="ACG808_002632"
     CDS             complement(4651..5049)
                     /locus_tag="ACG808_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFS5048219.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         1645 a         1042 c          769 g         1736 t
ORIGIN      
        1 gtacatactc ctttttatct attatttaga cttattccga cagctacatg aataacgtac
       61 aaatagattc gttactgatt tttcagagat actaccatgc gcagtgtgca ttgtcaatta
      121 ttcttgctct tattttccat ttgtgttcct gttatttcct gtttctcaaa tatgttagta
      181 aacaaaaata gatctggcaa agctacaact tcgccaggca atacagcact ctcgaatgaa
      241 atattcactc aaaaatctat cgttttattc aattttagat ctgttgcttt tattctttat
      301 ttcactcgat ggaagagaga acgaaaatag cttgctccta caatcaaaat gagtaacaca
      361 cccatccatg taattagctt gttcaaggtt ctactaccag tttctggtaa atcgcttttt
      421 ctattatctg tttctttcgc tttttcttga tgatcaattc cacgagaaac aaaatcaggc
      481 tgttttagca cactttttag gttgctccct gcatttctct tttgttcctt agttggtatg
      541 ataggtacca aatcaagttc attatttact tttcctgtgc tgctttcgct gctcgtgctt
      601 cctgtgctac tctcactact catgcttcct gtgctacttt cactactcgt actctccgta
      661 ctgctttcgc tgctcgtgct tcctgtgcta ctttcactac tcgtactctc cgtactgctt
      721 tcgctgctcg tgttttctgt gctactttca ctgctcgtac tctctgtact gctttcaccg
      781 ctcgtgtttt ctgtactgct ttcactactt gtgctatccg tactgcttgg cggtccaaca
      841 actgtccaac tgcttggttt caaattctcc gtgatcgtca tctgtccatc ataaaaaaca
      901 atcaaatcaa ctttatcttt actcttgatc tcatcaatcg atagttctgc agaaatcatt
      961 attgtatact caataccagc ggtccattgt acgtctttta aataaacttc cccaataact
     1021 ttttctgaac ttccattacc atcccaacca tatgttagaa cagcatgtac atcactagca
     1081 ttcacgcttt tgtttggttt gactggaata gtaatgactt gttgtgtcat gtcataaaca
     1141 atccgatcag ttttcacagt aaatgaaaga ttctcatttg cccgttcatt tgcagaagta
     1201 ctgattccaa tcattgctgc aattatactc agcccaagaa taacaaataa tacggtcgct
     1261 gttttcttca tttctatcac tctccatata attaccttct atttgataaa ggaattatat
     1321 aaaatgaaaa tgatgagaac acgacagtca gttgtgaaaa tctaacaaaa gatgtcgtgt
     1381 attcgcttga atttgtttac tttttcgttt aatcaatgac agacttaaaa aagcaagacc
     1441 gatatcagcc ttgctttctt catcttttat tgataaacag cagtgtattt cttcatccgc
     1501 tttgttttat atgcatagag taacaaacca acaatcaaga ctaacaagaa taatgtagtt
     1561 gatatagatg cttcagcccc aaagtttccc ccagtaatta ttgtattttg cgattggatc
     1621 agagtggaga aaaaaccttg ttcttgcgtt ccgctgactg gtatacccaa aatagctcct
     1681 tgtgtccaat tccaagtaat atgagcacca atagccgtaa ataaacttcc tgtactgtag
     1741 ataagtgcgc tgaaaaagca acctgctaaa aagatattga aaactgccag aaatgtcatg
     1801 ttaggattca atccgtgcaa aagagcaaat aatgcagcag atccaatcat tgctattaac
     1861 ggaccaaatc taagcatctg atgctgaaaa aagccacgga ataaaagctc ttctgatgcc
     1921 cccactgcaa taaataaaat aaaactcgtt atcagcggca caatgtttgc gtttcctgaa
     1981 tctaaccgat acgcccccgt caaatataat ggactagtaa taagcagaaa cactccaatt
     2041 ccaacaacga gacctcccgc taaatacccc cagtcttttc ctcgaacaga aacaccataa
     2101 tcttctattc tttttccttc gaccttacta gcaagaaata aagctagtaa aaaaacagct
     2161 aaatgtccga ccgtaccggc aattgctcca atccattcac tatttccaat aaacatatcc
     2221 gcaagtgcat tattgacaag aattcctaaa acaaaaccaa ctaagactat taaaacagat
     2281 ccaatcaacc gaataatcct gatcatcttt catccctcct ttatattaag tttaacaacc
     2341 cttttctttc ttgtctcgtg aaaactatgt aaatgttctc catcatagag agaatagatt
     2401 tttacgaact aagaatatct acagcaacct tacgaaaatg aaagactgtc tccctaattc
     2461 ttctgttaga ctgtttttaa taaggaaagg atgggaaatt ttttgaaaaa acaactacta
     2521 attatgtgcc tttcagcatg tgcaatttta ctattctcag gttgtagcaa gtccaatgaa
     2581 acgattgcaa cgtttaaagg tggaacactt acagttcagg atttttatga taacccaaaa
     2641 gttaaatcaa ataatcaaac actattaaaa aaaatgattg tcttcaaagc atttgatgat
     2701 gtatacggaa aagagatttc ttcaaaggaa gtaacaaaag aatataatga acaaatcaaa
     2761 aaactcggtc caaactacaa ggaacaatta aaagcagttg gccaaacaga agagacatat
     2821 aagaaactct ttaaacagat gctggctttc caatacggtt tgaaagccaa tgtaaaatta
     2881 acagataaag atctagacac tgcttggaaa gaattctatc cagaagttag tacacagatc
     2941 attctatttt cgactaaaga agaagcagat aaagcaaaaa aagaagcaaa tgaaggagaa
     3001 aatttctcca agcttgttca agcttatgga aaaaataaaa cattaaagga aactgatgga
     3061 aaaatgaatt ttgattcaac caatccagag attcctactg aagtaaaaaa agcagcattt
     3121 aaactaaaaa acggggaagt ttctgatatc attcctgtaa cagatccaac gacttatcag
     3181 caaagctatt atctagtaaa aatggtaaaa aaacaggata aaggttctaa taaagacaaa
     3241 tacaaatctg agctagaaaa aattgctacc gaagcaaaaa ctcttgatac ggaattcatg
     3301 gataaaacaa tccgtaaagt gatgaaaaaa gacaatgtaa ctattaaaga tccttatgta
     3361 aaaaatatat tcaaataaac aaatttgacc caattgggga tgtggcaaaa tttgagaagt
     3421 gatttttgga aattccttct tatttttgag gcaaaccact tttgccacaa cttcatcggg
     3481 gccttttttt atttgtacca aaacaaatca ctggatttgt tacaatcatg aggatataat
     3541 ttaaaaaagt tttttatttc tatcacatga atcaatgcgc cattaagtag attctttcat
     3601 gcttccctat tttttcactt gccgtatcgc ttcaaaaaca actatcataa actcaaactc
     3661 gactgaaaag ttattttaaa aaagactaaa aatatccctt ctggacttgt tcttcttatt
     3721 ctgttatact agagaaacac gaaagatatt tttatgcgac acttttcatt tgtttttatg
     3781 aaatttattc aaaactggcg taacttcaga gttacaagga tgtattagag gttagggaat
     3841 acatcgttta aatgagaaat aagtaatagt tagtaatagt cacccaaact ttaaattaat
     3901 gaatattgaa tcgtcagata gcctcctcga gttttcgaga aggctatcgt ttattttatg
     3961 gagaaattga ttcagaagta atcagcgacc ttttttagtt caccaatcgt ttctgcttgg
     4021 aaagtttgca cccaatcttc ttggaagccc ctcactgctt tatcaatcga gggcatctct
     4081 aatacttgat ctagtagctc tttacctaac gttatcgcat gagctccaga tcgaatggct
     4141 gtagtgactt gttcacaatt tttgaaacta gctgctaata tttttgttgg tctttgcgta
     4201 cgttcgatct cagtccgcaa cgaatcaatc actttcatag catcaatgcc ttgattttgc
     4261 atcctgttat aataaggtgc aatataatct gctccattat taatagctaa atacccttgt
     4321 aattctgtgt aaatagctgt accggtaatc aaataacctt cttttttcaa ctttttgatc
     4381 gcttgaaggc ctgcttgatt caccggcacc ttgataaacg tatcttttcc cagttcttct
     4441 atgataactc gagcatcctc tatcatttct tcttccgttc ttcctattgt ctgaatatgg
     4501 ataggaatct gtccgattat ttttttgatt tctttcatat gcgagaagaa gtttgacgct
     4561 ccttcttttt tcagaattgt aggatttgtt gttacgcctg ttaaattcaa aaactgttga
     4621 caagaataaa tactttttgt atttgctgta tcaagcatga tttccatata gctttccccc
     4681 attctgatcc tcttgatcga ttttaatgat tgtccccttt cttaaatgcg gcatttcctt
     4741 atcttctaca tacattgttc ctggaagttc cggctcatga gatccatcaa attttatcgt
     4801 gatatgtcct aattcattca aattagtttg gacaacatct cctactgctg tgattacata
     4861 catttgttta tcaaaacaga gcttcatatc acaagttatt tcttttctta cctgattcaa
     4921 ttcaatctta taacaataat ctgttaatgt ttccggtgct tcatctccaa ataaaatcat
     4981 tattttttct ttcctgaata cttctgcttc tataccaaca tctgttacaa ccgtttcaaa
     5041 gatcgtcatc tctgttcctc ctcgtaaaat atcatttgtc taatagtttt tattcaaaat
     5101 gtttctagat tataaaattt attgaataac acattcctaa cggaatttgt ttcgatattg
     5161 tgtgatttct cagagtacaa tggatgtatg ga
//
LOCUS       DAZUMK010000155         5059 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000155 DAZUMK010000000
VERSION     DAZUMK010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.160"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            92..1807
                     /locus_tag="ACG808_002633"
     CDS             92..1807
                     /locus_tag="ACG808_002633"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5048220.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            1810..3573
                     /locus_tag="ACG808_002634"
     CDS             1810..3573
                     /locus_tag="ACG808_002634"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5048221.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            3738..5033
                     /locus_tag="ACG808_002635"
     CDS             3738..5033
                     /locus_tag="ACG808_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS5048222.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT         1527 a          998 c         1000 g         1534 t
ORIGIN      
        1 cttgttctct tttcattcta aatttcctaa gctacaatgt ggtagtactt gttttttcaa
       61 ttgtggacaa ataaataata ggaggaatca tatgattcag ttattacgtt atgcaaaaga
      121 ctatcgtaag caaatcattt tgggcccgtt cttcaaattt cttgaagctg tcttcgaact
      181 tgtcctgcct ttaatgatgg cttccttgat cgataatgga ctaaaaatga atgaccgagg
      241 aaaaatcatt gaaatgggct tatggatggt cgctatgtct gtgattggtc tgatttgtgc
      301 tatcatttgc caatactatg cttcagtagc ttcacaagga tttggtacag agttacgtaa
      361 tcagctaatc aaaaaaatca atactttctc tcacaacgag ttgaatcatt ttggaacaga
      421 taccttgatc acccgaatga caaacgacat caatcagctt cagctagctt tagcaatggt
      481 cattcgttta ttgatcagag caccgttttt aagtatcggg tcagtggtca tggcttttgt
      541 gatcgactgg gaagtaggtc tgtttttctt agcattattg cctattttct caatcatttt
      601 attttttatc atcaaaaaaa ccgttcctct ctaccaaaaa gtgcaagaaa aactagatca
      661 gctcaatgaa cacgttagtc agaatcttag cggtgttcgt gtcatccgcg catttgcgca
      721 gacaaaaaaa gaaacagaga agttcgataa aagcacaaat gatctagcga acaattattt
      781 acgtgtttcc aatatctctg ccctattatc acctgctaca accttgatca tgaatgtcgg
      841 gatcatctgt ttgctgacag ttggcgggat caaagtaaat attggtagct tgcaacaagg
      901 acaagttctt gctttgatca attatatgaa tcaaatgctt ttggcactga tcgttgtttc
      961 aaacctcgtc gtgatcttca cacgtgccga agcttcagga aatcgggtga aagaggtgct
     1021 agacacagaa aattcgattc ttgaagctga ggtcagtact actcccgatt tttcttctga
     1081 agcaattatc gcttttgacc atgttgactt ccgttatact cctgattctg gtctttctct
     1141 ccaaaatatt acatttcagc tgaagcgaaa tacagtcctt ggaatcattg gtccaaccgg
     1201 aagcgggaaa acgactttga cacaattgat tccgcgtttc tatgatgtca gttccggtac
     1261 agttataatg gacggaatgg atgttcgttc ttggcagctg gatcatctcc gtcagcaaat
     1321 tgctcttgtt ccccaaactt ctgtactatt taccgggact attcgtgaaa accttcaatg
     1381 gggtaagaaa gacgccacag aaaaagaatg ttgggaagct ctgaaaatcg ctcaggcaga
     1441 agaatttgtc cgtcagcttc caaatggttt agatacaaaa gtcatggaaa atgggaaaaa
     1501 tttctctggc ggacaaaaac agcgactgac gattgcaaga gctttgattg ccaagccagc
     1561 attactgatt ttagatgact ctttaagtgc gttggattat caaacagact tggatttgcg
     1621 taaggcgcta cagacttatc ttggcagtac agtgattatt atctcccaac gtgtccgctc
     1681 tattcaagaa gcacatcata tcttagtgat ggatcaaggt aagattgctg ctcagggtac
     1741 ccatgaagaa ttacttgctc aatcagcaga atatcaggaa atcgtcgcat cacaggagga
     1801 agaatagtta tgaaagtaaa aaaattatcc ttgcagacgc tccgccgatt cattccttac
     1861 cttactgcct atgcaaaaga catttggcta gctgtgatac taggaatcat caatgggatt
     1921 gcaacagttg tgatgacgtt acaaattggt caaagtatcg atcaaatgat tggagaagga
     1981 caggtccgct tcaatcgact gttccattta cttattctat tcggtggtat cgttttaatc
     2041 aacgtagtca gtcaatggct catccaagtg ttaagcaatc ggttagctta cttatccgtt
     2101 gcaaaattaa gaaaagatgc atttgctcat ctgaatcgat tgccacttcg ttattatgat
     2161 caaaactccc acggaaatat cgtcagtcga tttacaaatg atatggacaa tatttccatt
     2221 gcaatttctg cggccttcaa tcaattattt tctggagtgg caatcatcat tctttcgttt
     2281 atttttatga tgcgtctaag tccacttctg acattggtcg ttctttgttc tacaccaatt
     2341 attttccttg tgagttggat cgttgctcgt gcttcacaaa caaatttcga tagacagcaa
     2401 aggattattg gggatatctc gggctttgtg accgaacgaa tcggtaacca gaaaatcgtg
     2461 aaagcttttc agcaagaaga agctagtcaa cagcaatttg aactgctaaa tgctgatcta
     2521 tatgaaaaag gacaacgggc acaattttct tcttccttga caaacccgtc ttctcgtttt
     2581 gtagatcacc ttgcttattt atcgattggt ttagttggcg gcctattggc attgaagagt
     2641 ggttcgtcaa tcactgttgg tgtgatttcc agctttacga tctactcaag tcagttttct
     2701 aaaccattta tcgaactttc tggaatcacg acacaactgc aaaccgcttt ttctggtcta
     2761 gaacgagcat ttgatttgat cgatcagcca gaagaaaagc ctgatttgcc agatgcctat
     2821 gccttggaac ccaaaaatat caaaggaaaa gtctcttttg atcatgtttc tttttcttat
     2881 gaccccaagc agcgtttgat cgaagatttc aattttactg cttatccagg tcaaaccatt
     2941 gctatagtag gaaaaacagg ggccggaaaa tccacacttg ttaatttgct gatgcgtttt
     3001 tatgacacaa caaaaggaac aattacagtc gatgaccatg acatccgctc tatccaacgt
     3061 gatacattac gtaaaagttt tggaatggtg ttacaagata cttggctatt tgacagtaca
     3121 ttgcgagaaa atcttcgttt tgggaatccg gaagcaagtg acgcagaaat caatgatgcc
     3181 ctaaaagcct cttatatgta tgaatttgta gaacgtttgc cggaaaaatt agatactaag
     3241 attggcggtc aagggttaaa gatatcagat ggacaacgcc aactgttgac gattgctcga
     3301 acgatgatca gtaatccccc tatgttgatc ttagatgaag ctaccagctc agtcgataca
     3361 ttgacagaga aaaaaatcca acaagccttc ttagcaatga tggaaggaaa aaccagtttt
     3421 gtgattgccc atcgcctgtc cacaatcaaa aatgcggacc agatattagt gatggatcag
     3481 ggagctattg tcgaaatggg cacccatgat gagctgcttc aaaaagatgg ttattaccga
     3541 cggctctatc aagcacaatt cacagcacaa taaaaacaag cagtcttctg ggatgggaag
     3601 actgcttgtt ttttaggctc tatacttttt gatgttggtg aacttcacca acatcttttt
     3661 tgtataaaaa aatatagaaa agacaccctg aaatccttta gaattaattt ggctaccaat
     3721 tcataggagg attcaagatg tctcatactc agcttatcaa agatacccta aatattctag
     3781 acctaaatat ctatttcgag gaaaattgtt taactaaaga aaaatacaaa gggcaaattt
     3841 gcttgattta caaaggaact cttctgtaca agccagaaga gtgttcccat tgtctttgtg
     3901 tcgtcccctc taggatcatt cgttggggga cgacaaccgt tcggttgtta ctgaatgatg
     3961 tgtctgagta tcgcacttat ctggagctga agaaacaacg atttaaatgt aagtcttgcc
     4021 aaagaacatt cgtagcggat acctctgttg ctgaaaaaca ctgtttcatt agtcaaaaag
     4081 ttcgttggtc tgtcattgct cgcttaaaag agaatacttc catgacggaa atcgctcgtc
     4141 agaaaaatat ttccacttct tcggtctatc gtgtcatgaa gcgattctac cgcccattga
     4201 atcctttcaa acagactttg ccaaaggtct tatgctttga tgaattcaaa tcggttcgaa
     4261 gtgttgccag tgcaatgagt tttatcatga tggacggaca aagccatgca ttattggata
     4321 ttgtcgaaaa tcgacgcttg ccttatttag aacgctattt ttctcggttt tcattagcga
     4381 ctcgtgaagc cgttcaattg attgttattg atatgtatgc gccttatgtt tcgttggtga
     4441 aaaagctttt cccgaatgct cagttgatca tcgatcgttt ccacatcgtc caacatattg
     4501 gcagaacttt cttgaatcat cgagtgaaag aaaccacggt acgacttaaa ggaaattccc
     4561 actctcaacg agggttaggg aaaaaattga agcgctattg gaaactccta caaaaagaag
     4621 aagcaaagtt aaactatgaa aaacgggttt ggcgtgccag ttttaaagat tatttgactg
     4681 aaagtgagat tgccgatcga ctgttagctg cttgtcccaa cttacgccaa ggctatcagt
     4741 tataccaaga cattctttat gcggtaaaga aaagagatca gtcactattt gaggattgtt
     4801 taactagaga cattagcggt ctgccagaaa cctatacaac cactttacga acgtttaaaa
     4861 agtttctgcc acagatccaa aatgcgcttc actacagcta ttcaaacgga ccattggaat
     4921 gcttaaacaa ccacatcaaa gtattaaaac gcaatgcgta cggctttcgc aacttttaca
     4981 atttcaaact acgaattatg attcgtcatg gcaaaacatt tttaaccaaa taaacagaac
     5041 agaaagaggt tttcttctt
//
LOCUS       DAZUMK010000156         5007 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000156 DAZUMK010000000
VERSION     DAZUMK010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.161"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..182)
                     /locus_tag="ACG808_002636"
     CDS             complement(<1..182)
                     /locus_tag="ACG808_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS5048223.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ACG808_002637"
     CDS             517..951
                     /locus_tag="ACG808_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS5048224.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ACG808_002638"
     CDS             974..1294
                     /locus_tag="ACG808_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048225.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ACG808_002639"
     CDS             1435..1626
                     /locus_tag="ACG808_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048226.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="ACG808_002640"
     CDS             complement(1697..1954)
                     /locus_tag="ACG808_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFS5048227.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="ACG808_002641"
     CDS             2112..2375
                     /locus_tag="ACG808_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048228.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="ACG808_002642"
     CDS             2399..2587
                     /locus_tag="ACG808_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048229.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="ACG808_002643"
     CDS             complement(2670..2855)
                     /locus_tag="ACG808_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048230.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="ACG808_002644"
     CDS             3070..3441
                     /locus_tag="ACG808_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS5048231.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="ACG808_002645"
     tRNA            complement(3591..3663)
                     /locus_tag="ACG808_002645"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="ACG808_002646"
     CDS             complement(3709..4137)
                     /locus_tag="ACG808_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048232.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="ACG808_002647"
     CDS             complement(4197..4955)
                     /locus_tag="ACG808_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFS5048233.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT         1742 a          784 c          978 g         1503 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaattctag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt attttta
//
LOCUS       DAZUMK010000157         4875 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000157 DAZUMK010000000
VERSION     DAZUMK010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.162"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..124
                     /locus_tag="ACG808_002648"
     CDS             <1..124
                     /locus_tag="ACG808_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFS5048234.1"
                     /translation="STTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
     gene            175..291
                     /locus_tag="ACG808_002649"
                     /pseudo
     CDS             175..291
                     /locus_tag="ACG808_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341329.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YoeB family
                     toxin"
     gene            368..697
                     /locus_tag="ACG808_002650"
     CDS             368..697
                     /locus_tag="ACG808_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS5048235.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            687..974
                     /locus_tag="ACG808_002651"
     CDS             687..974
                     /locus_tag="ACG808_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS5048236.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            1260..2117
                     /locus_tag="ACG808_002652"
     CDS             1260..2117
                     /locus_tag="ACG808_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFS5048237.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            2118..2666
                     /locus_tag="ACG808_002653"
     CDS             2118..2666
                     /locus_tag="ACG808_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048238.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            3442..4811
                     /locus_tag="ACG808_002654"
                     /pseudo
     CDS             3442..4811
                     /locus_tag="ACG808_002654"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
     gene            4775..4875
                     /locus_tag="ACG808_002655"
                     /pseudo
     CDS             4775..4875
                     /locus_tag="ACG808_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT         1760 a          661 c         1006 g         1448 t
ORIGIN      
        1 ctctaccacg attaaaggca tggaagccat tcgaggatta tataagaaaa cccgaaaaga
       61 aggcactctc ttcgggtttt cggtctgtac tgaaatcaag gtattattgg gaatcccagc
      121 ttaaatcata gataccgtaa gggattttat tctttattta aaactttgca acagaaccaa
      181 ggtaggtatt ttagaagatt gaatagacaa caccgggttg tttataaggt agatgaagat
      241 gaacacgtgg ttgaaattta ttcagcaagg acgcattacg agtcacagta attacttgta
      301 aatacattat ctcgtggtat aatgtattta ctaagtaatt acatactgtt agaaatgagg
      361 gatttaaatg attacaaaaa caagaaaaca aggcaattca atcatgttga cagtacctaa
      421 agattttaat gttcctaatg gagtcgaagt tgaagcaaag ttggtagaga atggtattct
      481 ttatgaattt gttgaaccac aaaaagaatt ttatgatttt agcgaagata ttttgtctga
      541 tataattgca gaaggctacg ataaagatga gattcttgta gaatttaaga atagaaaaaa
      601 caaaatgcat tcttccttta gagatattgc tgaagataca cttactaatt ctaaagtaat
      661 gactaaagag gagttggcaa cagaaattgg cttatgaaat cgttccttca aaacatgtca
      721 ttaagtattt aaaaaagcta aaagagaagc cattgaaaga aaaatttttg aatttaattt
      781 atgatgaaat tgcgattgat ccatataagg gtgaacaaaa aactggggat ttatcaggta
      841 tttggtctag tgggttcaaa tatgcgggca caacatatag agtagcttac gagattatag
      901 aaaacaaggt tattccagta ttgttatgtg gaacgcatga aaacttttat gaacagttga
      961 aaaaattaat ctagtgattt gttgtcgttc ttaagaagga cggcttcttt tttatcgaaa
     1021 atctgattcg tctacatgac taaagtcgcg agtattctct gcttctcgat aaattaaata
     1081 acgtcattac tttgaggaaa gctataaatc aatatatttc agcttttcct tatttatgta
     1141 ctacctttta cttattaatt ataaggtagt acatatatat cttttttttg gtagtaacta
     1201 aaaataaata tgttataata gtatcaaatg aatattgcta aaagaaaaga ggtaggaacg
     1261 tggtagcgaa gaaatatgta atcggtaatt ttaaaggtgg cgtaggtaaa agtacgtgcg
     1321 cgcaaatgtt agggtttgaa tcagcagtta atgaaggaaa aagaaccttg ataattgact
     1381 tggatatgca aggaaatact tctgatgtga tgaacttaac gcatatgaac ttcactaacg
     1441 aagaaggagg cggagaaggt gaaccgattg tttacgaaaa tactgtgact gacgtgttga
     1501 taaacaatct tccacctaaa gaggctgtct ataaagtaat caataacctc tatatacttc
     1561 cagcagacat gtcctttgaa atgtatgatg attggatcaa agaccaatat aatgattcat
     1621 tatcccaatt caaatatatg aaagaaaagc tagaaccatt atttgatgag tttgacgtca
     1681 tctatttaga tgtcccacct tctatttctg tatatagtaa atccgcgatg tacattgcgg
     1741 actgggcaat cgtcgtctta cagacccaag taaaatcgat gagaaatgcg atgcaatatc
     1801 ttgagtatat ggactttttt gtgaaagaat ttgatacaaa tttatatgtt gctggtgtta
     1861 ttcctttcat gttggaaagc ggtgacgcag tagatcagga aatgtataaa caagcccaag
     1921 agatttatgg ggaacatttg attaaaaatg ttgtactcaa aaatgcacgc ttaaaacgat
     1981 acgacggatc aggtattacg atggagaaga caaagaaagg tctcttaaaa caatgggatc
     2041 gcaaaagcca cgaattattt atcagtattt tgaaagaatt ggaagaacac gagaattggt
     2101 atgcggaggt ggagtaattg gctgacatca aaggacttga tatttttggg aacaacaaag
     2161 gactcgccaa aataaaaaaa gaagcagaag caaagaaaaa gcagcaagca attacaccat
     2221 ctgaaaataa gcaattagaa gtgcaccaag ttcaagaaat aaaaacttcc tccaatgttg
     2281 aaagacagag tagaggaaga acaaaaaaag ttggcgcacc aatcagaaag tttgataagg
     2341 tttattccac caagcatcct ttaaaactgt cgactttgtt aaatgcgact tccagagttt
     2401 tagtagaaaa gtatgagact aatatgacaa gagatgaatt gcttagaaaa gctttagatg
     2461 agtatattaa acagaatctt acgcaagaag ataagcaaga tttgtttaat gacgtggtga
     2521 aagaactaga cttgttcaga gaaaaatatc caattgttcc agaattagat gatcgtggag
     2581 aaataataag aactaccagt cagataaaac aagataccga agactcactg cgcaaagggt
     2641 ggggaatgaa taataaagtt agataataat caaagaagaa ctaagtaact acctttaaac
     2701 ggtagttact tagttcttct ttgatttgcg tgaaatagag gggatttatt tttctggtta
     2761 cgtgattaaa aaatccaaaa aatgagcata ggaacggtta tgattttagg gtgtgtttta
     2821 atgagatatt gtgatttcat taacaagtgt atgataatct gttgttgagt tagtaaataa
     2881 gtaatagagc taactctaaa ataaaactat taggaggaga tgtaggtgaa aaagtttaaa
     2941 aagaaaatat atttaggatt ggttgttggg gtgagtatgt ttgggttcat ggctcatact
     3001 aaacaagtaa ctgcagatac taaccaaaaa gaaaggatta tgagccaatt agatgactct
     3061 caaatagagt atgtacccaa tgagctaatt gtcagtttaa aggctacaca aagaatttca
     3121 acagagaaaa ataaaccgca aaatttattg ggtgtaaaag ttatggtgga aaaagatttg
     3181 actcttagga caaatgaaag cgattataat aagcgagatc aagtattatt agtgaaattg
     3241 aacgaagaaa ttagtgtgga agaggcagta agaacattta atgcttctga tatggtcaat
     3301 tatgcagagc caaactatgt ggttgaacta gagccgttga atgatgttga ggagccaaat
     3361 acaactgatt ctttaggaaa tcgatcaatt attcttaacg atcctaattg gagtaaacaa
     3421 gctaatttaa gagagattag catgccatcc gcttgggaag aaaccaaggg ttctaaggat
     3481 gtgaaggtag cggtcattga ttcaggaatt gattatcaac atgaagatct ttctggaaat
     3541 gtagatgaaa gtttgagata cgattttttt gacgatgata cagatcctat ggatactatg
     3601 ggacatggac acatattgcc gggattattg gtgctcaaac aaacaacgaa ttaggtgttg
     3661 ctggaataaa ttgggatgtt acgatggtac ctatacgtgt atttggtagt agtggaggta
     3721 ccagtttggc aatcttaact gaagctatta attatgcatc ccaaaaaggg tttccaatta
     3781 tcaattttag tataggtagt gttgtttata gcagtacaga agaagcaatt aaaaactatc
     3841 ctggattatt cgtagcagca gctggaaata atgggataga tatgactgat aaaccttttt
     3901 acccagcttc tttggatata cctaatatta ttactgtagg aaattctagt ggttatgaac
     3961 gaagttctga ttcaaattat agtaaaacag gtgttgattt attcgctaga gggtttcgtg
     4021 tttttagtac atttctagga agtaaataca cgtatatgtc aggtacctcg atggcaacac
     4081 ctcatgtcac tggtagtgct gcattagcgt tgtcactaga tcctgatcta tctactgatg
     4141 aattaaaaaa aatcatttta aatagtgttg attcatttta tgaactatct gatttatgtc
     4201 ttacaggggg aagactaaat acgaataatg tcgtacgaca agtaacgggt aaacttactg
     4261 catctaatat cacattaaga ttaaatagtg aatggtcaga tgaaattgct aaagagatgt
     4321 gtcacgttca ctggattgac cagtcaggaa atgatttgac ggatcaagtg gttgttctaa
     4381 aaaacgaagt ggatacatct aactttggcg attacgaagt agtatttgca tcacctgatt
     4441 taacagatca aatctcagtt acagtgaccg tacctcgatt acgtaaactt acgctaggaa
     4501 aagaaacagc aaccattgat attggttccg catgggatag tgtcatagcc attgatactt
     4561 ttcaagtgaa agctatagat gattttggga atgatgttac aaatcaagta caggttacta
     4621 ttaatgctga tatagataca attaattcaa ttcaagcagt gattaagttt gaagttccag
     4681 aattaggctt agcccaaatt ggtagactga atattactgc aaaaaatagt acaatcaatg
     4741 gattaaatga agttagcatc gagcacgtaa aaaagattat gtcgttttcg aagaagaatt
     4801 tggacattta gagggtgata cgattgtcgg catccaccat aaaagtgtcg tcatcacact
     4861 cgttgagcga ctttc
//
LOCUS       DAZUMK010000158         4831 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000158 DAZUMK010000000
VERSION     DAZUMK010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.163"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            20..232
                     /locus_tag="ACG808_002656"
     CDS             20..232
                     /locus_tag="ACG808_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048239.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            complement(209..1819)
                     /locus_tag="ACG808_002657"
     CDS             complement(209..1819)
                     /locus_tag="ACG808_002657"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFS5048240.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(2041..2286)
                     /locus_tag="ACG808_002658"
     CDS             complement(2041..2286)
                     /locus_tag="ACG808_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048241.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            complement(3012..4352)
                     /gene="glnA"
                     /locus_tag="ACG808_002659"
     CDS             complement(3012..4352)
                     /gene="glnA"
                     /locus_tag="ACG808_002659"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFS5048242.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDSGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(4380..4772)
                     /locus_tag="ACG808_002660"
     CDS             complement(4380..4772)
                     /locus_tag="ACG808_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS5048243.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         1429 a         1042 c          792 g         1568 t
ORIGIN      
        1 tagttttcta ctaaaatcaa tgaaaatgaa aattttcaag aagttttttg caaaaaataa
       61 aatatccaat agcgctttaa tcgaaaaaag tcttttgctt tctatctttt ttcctacaaa
      121 taataacaaa tcagaaaaag agaaaagtga caagaaaatt gcttatcccc ttctcttgct
      181 acttttctct ttctatcaag ttcaaaaatt attttgtttc gtgatctgct aatgatgctc
      241 cgacaaatgc tttgattaat ttttgcgggc gatttggacg agaaatcaat tctgggtgga
      301 attggcatcc cacaaagaat tttttctctg gcagctctac gatttctact aaacggttat
      361 ctggtgagac acctgaaaag actagccctt gttcttcaaa caattgacga tatttgttat
      421 tgaactcata gcggtgacgg tgacgttctt gcacaacatc ggctccatca tatgcttcag
      481 ccgtcttcgt tccttttttg attttacatg ggtataatcc aaggcgtaaa gttccaccca
      541 aattctcgat gttttcctga tcagccatta aatcaataat attgtttgct gtatctggat
      601 tcgtttcagc tgatgacgca tcttctaatc caacgacgtt acgtgcaaat tcgacacaag
      661 ccatctgcat accaagacaa atacctaaaa acggaacgtc attttcgcga gcataacgaa
      721 tcgcttcgat tttcccttct actccgcgat caccaaatcc accaggaacc aagattccat
      781 ctgcgtcttt caaacgttct tctacatttt ctctcgttaa ttcttgtgct ttgatccaat
      841 cgatctcgat gtctgaatta taggcgaaac cagaatgttt caatgcttca actactgaga
      901 tatatgcatc tggtaactcg acatatttac cgacaagcgc aatccgtact tttttcttca
      961 agttcaagac acgttcttct aattctttcc attctgtcat gtctgcttct ggtgtatcta
     1021 gttttaaatg gtcgcaaaca atttgatcca ttccttgtgc ttgtaaaact aatgggatgg
     1081 agtaaagtgt atctacatct ggcgactcga taactgcttc tggttcgaca tcacaaaatt
     1141 gtgccaattt gtttttcacg ctttgagaaa caggttgctc tgtacgtacc accaaaatat
     1201 tcggttggat acctaaacca cgtagctctt ttacactgtg ctgcgttggt ttagttttca
     1261 tttctccagc agctttcaag taaggaatca atgttgtatg gatatacata acattatctg
     1321 taccgacatc tgctttcatt tgacgaagtg cttcaaggaa tggcaaagat tctatatccc
     1381 ctactgttcc ccctacttct gtaatgatca catctgaatc agtcatttta gctgctcgca
     1441 tgattttttc tttgatctca tttgtaatat gaggaataac ttgaacagtt gctcccaagt
     1501 attcgccctt acgttcttta cgcaaaactt cagaatagat ttttcctgtc gtcacgttgg
     1561 agtatttgtt caaattgatg tcaataaaac gttcgtagtg gcccagatcc aaatctgttt
     1621 cagcaccatc atctgttacg aatacttctc cgtgctgata tgggctcatc gtccccggat
     1681 ccacgttgat gtatggatca aatttttgga tagttacttt taagccgcgg tttttcaata
     1741 agcgtcctaa agaagctgca acaatccctt ttccgattga tgaaacgaca ccgccggtca
     1801 caaaaatata ttttgtcata agttcaaaaa ctccttttta atttatgtgc aggaaaagtc
     1861 tcagacaata aacaaagctc cctattcttc aataagaata gggagccgaa atttctgtaa
     1921 acttctgacc ctctaaaagg gtgcccaagg tgtataatac aagctgacta agggaaagtc
     1981 aagtactcaa atgaatacta tccgcttaca attttttctt tttcgatatc ctttgagaaa
     2041 ttatttctta agtcctttat gagtgcaaaa aaaatttaac ccacataata agcttgatat
     2101 aaacatagct aacgttaaac ctaagcaaat cagaaaatcg ttactgaaaa attttaagtg
     2161 aaataataga taaaaaaata aaacaaacat tatgatagat aatatcccac ctaacaaaga
     2221 actacttttt cctacttgca gcaacagttt atatagctta tcgtagaaac aatctttttc
     2281 tttcaccctt atttacctcc tttacgtaaa aaacgtagtc cacagttctc ggatttttgg
     2341 catatctttt ctattaaata atatcaaaaa caaaacaatt ccactaattt tttcaaagat
     2401 gacctttttt tcaacaaaag tgccttgtag ggattcattg aaggtaacga atcacataag
     2461 taaaaaataa ggagatgcga caaaagtcgt gcctccttat ttctaaataa acggtgttct
     2521 cctgccaact cctcggtcac aagtcacaat atccatcaaa catgagaagc tgtttttgaa
     2581 attatttctt gtgattcgga gttaaatggt aggctcacaa cctctggata aacggtattc
     2641 aaaagccagt ttctcggtat taagccaaat ttatgcaaaa atgtagaaag ccattttcac
     2701 aaaatttttc ttaatactcg aaactaaacg tctttttcaa aacctcttgg taaacggtgt
     2761 tcaaaagctt gcatctgcgg taataagttc agccaattca aaaacaagaa aagctgtttt
     2821 tgcttggcta ttcttattac ttgttgcagg gcagcttttt cacaacctct ccactaaacg
     2881 gtgttcaatc agctgaccct cggtattagc tcaaaaaacg gcaaaatatg aggaactatt
     2941 tttccgttag tttctctaat actcaggtca aaacgctgat ttcacagcct ctaattcacg
     3001 gtattcctgt tttaataaag ttctagatat tgttctcttt cccactcgga aacagtttgt
     3061 ctgaatgccg cccattcgag acgttttgct tcaacaaaat ttgagaagat atgttgtcct
     3121 aatgcgtcaa tcattacatt atccttacgc aattctttga tagcattatg gatagttgaa
     3181 ggtaagtcct ggatatgtgc agcttctctt tcttcctcat ccattacata aatgttacgg
     3241 tcaacagctg ctggcggtgt gatctgatta cggataccgt ctagtcctgc ttgcaacaat
     3301 actgccattg ccaagtatgg gtttgcagat ggatcaactg accgcaattc caaacgtgtg
     3361 gataaaccgc gagattctgg tacacgtact aatggcgaac gattacggcc actccatgcg
     3421 acataaacag gcgcttcata acctggaacg agacgtttat aagaattgac tgttggattg
     3481 caaacggctg tgtaggcacg agcatgtttc agcaaaccac ctaagaaatg ataagctgtt
     3541 tcacttagtt ccaattcacc gtctttatca tagaaaacat ttccttcatc attgaacaaa
     3601 gacatattgc aatgcatacc agaaccattg atcccaaata acggtttcgg catgaaagtc
     3661 gcgtgaagtc catgcttgcg tgcaatcgtt ttgacaacga gcttgaaggt ttggatatta
     3721 tcacaagcat ctaccacatc tgcatattta aagtcaattt catgctgtcc tggcgctact
     3781 tcatgatgtg aagcttccac ttcaaagcct agactttcta attccaacac gatgtcacgg
     3841 cggcaatttt cacctaaatc tgttggtgca aaatcaaaat aaccaccttt atcattcaag
     3901 gtcgttgtga tcccactgtc ttcgtctaat ttaaataaga agaattctgg ttcaggtcct
     3961 aaattgaaag aggtaaaacc aaggtcgcgc atatctttca atgctcgttt caaattcccg
     4021 cgaggatcgc cggcaaatgg tgtcccatcc ggattataga tatcacaaat taaacgagca
     4081 acttttccat gagcactttc ccacgggaag atcatccaag tggagagatc tggatacaaa
     4141 tacatatcgc tttcttcaat tcgaacaaat ccttcaatcg aagaaccgtc aaacatcatt
     4201 ttattttcta atactttgtc gatctggctc actggaactt ctacattttt gatcgtacct
     4261 aagatgtctg tgaacatcaa tcgtaaaaat cgtacgtttt cttcctcaat gattcgtttg
     4321 atttcttcac ctgttatctg tttctttacc atggtatccc tcttcctttt ttaatttatt
     4381 cagtcaaaga attgtctttg caatccttgc gactgataat gacctgtttt gcttaaccca
     4441 ccttgcgcca aaatctcatc acgaaaaatt tgtcgaacgt catgatctgt caacggcttc
     4501 ttgttttttg cttcttgttt tttcgcctgt gccatttcgt atacacgttt gatcccagcc
     4561 atattcaatc cctctgaaag atagtcttta atctcaagca acatatcaat atcattcagc
     4621 gaatacatac gtcggtttcc ttcgcttcgt tccggatgaa ctaaatcttg ttcttcataa
     4681 tagcgaattt gccgagcaga caaatcagtc agcttcataa ctgtaccaat tggaaatacg
     4741 gacatagatc ggcgcaattc cttttctccc atcagacgtc ccctttcgtt ttataagttg
     4801 gctaaagtgt accaattaaa aaaaatattg t
//
LOCUS       DAZUMK010000159         4820 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000159 DAZUMK010000000
VERSION     DAZUMK010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.164"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(80..1888)
                     /gene="pepF"
                     /locus_tag="ACG808_002661"
     CDS             complement(80..1888)
                     /gene="pepF"
                     /locus_tag="ACG808_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFS5048244.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2064..3062)
                     /locus_tag="ACG808_002662"
     CDS             complement(2064..3062)
                     /locus_tag="ACG808_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFS5048245.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3288..3941)
                     /locus_tag="ACG808_002663"
     CDS             complement(3288..3941)
                     /locus_tag="ACG808_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFS5048246.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4273..4671)
                     /gene="spxA"
                     /locus_tag="ACG808_002664"
     CDS             complement(4273..4671)
                     /gene="spxA"
                     /locus_tag="ACG808_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFS5048247.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1418 a         1008 c          748 g         1646 t
ORIGIN      
        1 aaaaaatacg tcgactccct tacaagagag ccgacgtatt tttttcgttt ctattcgaga
       61 aaatcaaaaa tagaaagcct tataaacgat cgatcaattc ttctaattca tttagacgtt
      121 gttcaaacat cgacattgca tcttcaatat atgcagcctg tgtcatatca actcctgctt
      181 ttttcattac ttccacagga taatcgctgt tacctgcttt taggtaagcc aagtagttct
      241 ctagtgcctc tggttcctgg tttagtattt tcttcgccag tgctgaagca gcagaaaagc
      301 cagtcgagta ttggaaaaca tagtaattat aataaaaatg cggtatccgt gaccattcga
      361 atttaatttc tgggtcttct tcgactgctg gaccataata ttttgcattc aatttaccat
      421 aactatcact taggtattca ctagtcaatg gcacaccttt ttcatcttcc gtatgcatga
      481 aatgctcgaa ttcagcgaat tgtgtctggc ggaaaactgt tcctttaaac ccatccaagt
      541 aatggttgag tacataagcc cgtacacgag ggtctttttc tgtttccaat aaatactccg
      601 ttaggatatt ttcattcgtt gtcgaagcaa tttctgctaa aaagatggag tagtcgccgt
      661 acacataagg ctggttcgaa cgagtgaaat aactatgaac actatgtccc atttcgtgga
      721 caagcgtaaa tagctgatcc agcgtatcat gccaattcaa taaaatatat ggatttgtgt
      781 catagcttcc cgaagaataa gcaccgcttc gtttcccttt attttcgaca acatcgatcc
      841 aacgttcaga gaatgctttt tctacgatgg ccatgtattc ttcacccatt ggtttcagtg
      901 cttctaaagc tttttctttt gcttcttcgt acgtaaaggt aattggagct tcccctaaga
      961 ccggtgtata aagatcatac atgtgcagtt tttccacttc taataaacgt ttccgtaatt
     1021 ccatgtatcg atgtaacaaa ggcaaatgct tgtttaccac gtctaccaaa gtatcgtata
     1081 cactttcagg aatatgatta ttgctcaaag acgcttctct ggcagagctg taattacgga
     1141 ctttcgcttt aaaattatgt ccttttatat gtgtgcctaa agtagaagca aatgtatttc
     1201 taaattgttc gtaaacactg tacaacccct taaatgctgc ttcgcgcacc cttcgatccg
     1261 tgctttctag caactgacca tacacgccat gagataattg gactatttca ccattttctc
     1321 cttcaatcgt tggaaaaact agatctgcat tattcaaaac agcaaatgta tcacttgaag
     1381 catcaaagat ttcacctgct ccagcaagaa gagattcctg ctcagcagat aagacatggg
     1441 ctcgattatc tactatttgc tggatataat ggcgatagac ttccaatttt ggttcttctt
     1501 tgaaatattg ccaaatctgg tcatctgaca attgcaatat ttctggttca aaccaggaaa
     1561 cggcttcact aactttcgaa aacagactgc ttgctcttgc ataaagcgct tggtaatctg
     1621 tatttccagt gtcttgatcg tttttaaggt gcgcatatac ataaatgact tcagtttggc
     1681 gataaaccct caatacgaat tcaatcgcat ttaaaaattg agaagcgcct tgatcaagtg
     1741 ttcctttgtg tttttcagat tgttttaact cttctgataa ttccaaatat ttctcatcaa
     1801 actcttggtc gcttgaaaag atcttggtca agtcccaagt taaattttca ggcaattctt
     1861 ctcgttttgg caactgcttt acttccatga aatccactcc ttttcttccc ttagctctat
     1921 ctatcaaaat agatcatagt ctaacatcca catgtcacag catctaactt ttctactcat
     1981 tcatgtgtct gcttccatca attttagcat aactaaatct tgttttacat agaaaaccga
     2041 ttcctagcct atttttatac ttactatttt ttcaaagaag acaagtgtat gcattcgtta
     2101 aaaaactgct gatagaccat ctcttcatca atcataggaa aaagccattc ccttttatgt
     2161 tcttttattt ggcaaagcca tgagttgaaa tcaggaactt tctggttttc caaggcttct
     2221 tcgtacaaca ttcgataaag aaaaacctgt tcttgtaaat aaaagaaaaa ccgacttttt
     2281 tgatagatcc acgatgatag atgcaaaaga tgtttatgac gcaagtagca ttgttcttgt
     2341 atggatactg tattcttttg cttgtgaata agttggcgtg tcagccattt cttcccatct
     2401 tcactcactt tttctatttt tttcatttgg ttcattttaa tttcgttact agtgaatatt
     2461 tctttaaggt ccctactaga aaaagtccag cagctactgc aaaaggaaat atgccctgat
     2521 acagtctctg taatatgatg atacaaataa agcttttgtt tcttgagatc agctttccag
     2581 caatgcactc ctctttttct attatagtag cagcaacttt tttctatcaa tgagaattgc
     2641 gattgtccaa gaaaagaatt accaagtatc caccatactt ggtaactgtt attcaaataa
     2701 ttttgggttc tttcaataaa tctctggtga gacagtctag aacattggat ttctattgct
     2761 agattcgcca acaaaatatc cggcctttgc cgcaacctcg gcaaataggc ttccaaaaat
     2821 actggttcta ctgactgtcc cagccaatcc atgaagcatt cctttaactg taagtgctct
     2881 tttgattctc tttcactaaa gaccgaacag ttatctgatt tttgatgggc aaaatgagca
     2941 cattttttct ttcccttttt caaaatcact ctttttcgac agccacgaca ataatatttc
     3001 tttccttttt ctgcatgttt agcaaaacag cacttattat attcattcat tgcaagcagc
     3061 atttttctcc ctctgaactt atttacgtct aagaatttat ttacacgaaa aaagagggcg
     3121 tgaaaaagct attctgcatc aagtaataag aacagctaaa caaaaatagt tcccaatatt
     3181 tttcgtttgg ttgggcttgt taccgcagat gcaagctttt gaacacattt atacgaaaaa
     3241 aagactgcga cacaattttt gtcacagtct cttactataa tgctgattta tacaaaataa
     3301 aatttcgttc gctcgatagc attacgcgcc atcagacatt gtccatattc attcaatgtt
     3361 tctggtgtac gatcgctctt ttcagcaaac tccaaaattc gagcaatctg attctctaca
     3421 tccgcttggt tgagattttc catccagaag tgaacgacta ggtaatagcg ctcttcgaat
     3481 aaatacaaat cagtccaagc agactctagt tcgacatctg ttgcaagttg gatcacttgt
     3541 tcaaaatctt caaaagcaaa agttgcttgt ctttcctgtt cttctagggc gcttccagat
     3601 aattcttcct ccggatcgct gccaataatc tgctgacgaa gaaagtcact cacatcttct
     3661 gaatcaacag tagaatcttc tgattgagaa atttcttcat ccggaagatt tttactgata
     3721 aacagttcta atccgtctcc ttttggcaaa acttggaaag tgacagcttc gcttcctcgg
     3781 aactcctctt cgatatcaac ttcttccaaa atactataaa agaaattttc gatttctttg
     3841 tgattgccaa gtaaatccaa aaatgaaacg ccccgttcct ctaaatcttc atgaccaatt
     3901 agtacacgga tcgtattttc attaatatgt tccatttcca ttttgctaca cctcactttg
     3961 ccacaatgtt taatatagat tcattgtagc gaaatcagct taaatgtaaa gaaaaaaaat
     4021 ctcgctgtta agcgaattta ataaaaccag cggatttttc aacctttcaa gctatcttat
     4081 ttaactataa catttttggc agacgaatac tatatagccg acttttcagc taattttccc
     4141 tctctatttt tcgtgcaatc atcatcttct actacctttt tatacatcta atcaagaata
     4201 ctttttgtaa aaacaaaaaa acttggaaaa actatctttg taatagtctt ttccaagttt
     4261 cgtattatcc ttctataatc cagccatcaa ttgtgcttga cgtaattcta attgtcgtac
     4321 atcgcgcggt aggaaccgac gaatttcatc ttcattaaaa ccaacttgca gacgtttatc
     4381 atcaatcatg attggacggc gaagcaatcc aggattgttt tgcacaagtt ccaataattc
     4441 ttgtaacggc agatcatcta aatccatgtt cagtttttgg aatactttgg aacgtgtcga
     4501 gatgatttct tctgttccgt cttctgtcat ttgtaaaatc gcttttaatt cagaactgtt
     4561 caatggttct gaaaaaatat tacgttcaac aaatggaatc tgatgttctt gcaaccacgc
     4621 acgagccttt ctgcaggaag tacaacttgg ggaagtatat aatgttaaca acgtgcacca
     4681 ctccttttcg tgataagaat cacttttttg taacagaaga tcataataat ctcccatctg
     4741 tttctattat acataatcaa cttttaaata gctacctttt tttcataatt tttttaaaaa
     4801 aatgaaagtt atctaaggtt
//
LOCUS       DAZUMK010000160         4806 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000160 DAZUMK010000000
VERSION     DAZUMK010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.165"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..411
                     /locus_tag="ACG808_002665"
     CDS             <1..411
                     /locus_tag="ACG808_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048248.1"
                     /translation="GLYYSPVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAP
                     QEYLSRAYNDAPKYCANIVDTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLT
                     ELKGYHVTTGEPVCQPRRPNSRIDKVRYMFNKIT"
     gene            509..1882
                     /gene="tet(L)"
                     /locus_tag="ACG808_002666"
     CDS             509..1882
                     /gene="tet(L)"
                     /locus_tag="ACG808_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HFS5048249.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            2446..3708
                     /gene="mobV"
                     /locus_tag="ACG808_002667"
     CDS             2446..3708
                     /gene="mobV"
                     /locus_tag="ACG808_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="HFS5048250.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTGGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            3953..>4806
                     /locus_tag="ACG808_002668"
     CDS             3953..>4806
                     /locus_tag="ACG808_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFS5048251.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQRFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYG
                     GLLKEIHK"
BASE COUNT         1658 a          665 c          982 g         1501 t
ORIGIN      
        1 ggcttatact atagccctgt tagtacccca gcagattttc ggatgcttgc tcctattgta
       61 ttggaacaag tcttaaaaaa agctggaaca gaattgttag agccatatct tagttttaaa
      121 atttatgcgc cacaggaata tctttcacga gcatacaacg atgctcctaa atattgtgcg
      181 aacatcgtag acactcaatt gaaaaataat gaggtcattc ttagtggaga aatccctgct
      241 cggtgtattc aagaatatcg tagtgattta actttcttta caaatggacg tagtgtttgt
      301 ttaacagagt taaaagggta ccatgttact accggtgaac ctgtttgcca gccccgtcgt
      361 ccaaatagtc ggatagataa agtacgatat atgttcaata aaataactta gtgtatttta
      421 tgttgttata taaatatggt ttcttgttaa ataagatgaa atatttttta ataaagattt
      481 gaattaaagt gtaaaggagg aattaattat gaagtgtaat gaatgtaaca gggttaaata
      541 caaccaaatt ttaatttggc tttgcgtttt atcttttttt agcgtattaa atgaaatggt
      601 tttgaacgtc tcattacctg atattgcaaa tgattttaat aaaccacctg cgagtacaaa
      661 ctgggtgaac acagccttta tgttaacctt ttccattgga acagctgtat atggaaagct
      721 atctgatcaa ttaggcatca aaaggttact cctatttgga attataataa attgtttcgg
      781 gtcggtaatt gggtttgttg gccattcttt cttttcctta cttattatgg ctcgttttat
      841 tcaaggggct ggtgcagctg catttccagc actcgtaatg gttgtagttg cgcgctatat
      901 tccaaaggaa aataggggta aagcatttgg tcttattgga tcgatagtag ccatgggaga
      961 aggagtcggt ccagcgattg gtggaatgat agcccattat attcattggt cctatcttct
     1021 actcattcct atgataacaa ttatcactgt tccgtttctt atgaaattat taaagaaaga
     1081 agtaaggata aaaggtcatt ttgatatcaa aggaattata ctaatgtctg taggcattgt
     1141 attttttatg ttgtttacaa catcatatag catttctttt cttatcgtta gcgtgctgtc
     1201 attcctgata tttgtaaaac atatcaggaa agtaacagat ccttttgttg atcccggatt
     1261 agggaaaaat atacctttta tgattggagt tctttgtggg ggaattatat ttggaacagt
     1321 agcagggttt gtctctatgg ttccttatat gatgaaagat gttcaccagc taagtactgc
     1381 cgaaatcgga agtgtaatta ttttccctgg aacaatgagt gtcattattt tcggctacat
     1441 tggtgggata cttgttgata gaagaggtcc tttatacgtg ttaaacatcg gagttacatt
     1501 tctttctgtt agctttttaa ctgcttcctt tcttttagaa acaacatcat ggttcatgac
     1561 aattataatc gtatttgttt taggtgggct ttcgttcacc aaaacagtta tatcaacaat
     1621 tgtttcaagt agcttgaaac agcaggaagc tggtgctgga atgagtttgc ttaactttac
     1681 cagcttttta tcagagggaa caggtattgc aattgtaggt ggtttattat ccataccctt
     1741 acttgatcaa aggttgttac ctatggaagt tgatcagtca acttatctgt atagtaattt
     1801 gttattactt ttttcaggaa tcattgtcat tagttggctg gttaccttga atgtatataa
     1861 acattctcaa agggatttct aaatcgttaa gggatcaact ttgggagaga gttcaaaatt
     1921 gatccttttt ttataacagg aattcaaatc tttttgttcc attaaagggc gcgattgctg
     1981 aataaaagat acgagagacc tctcttgtat cttttttatt ttgagtggtt ttgtccgtta
     2041 cactagaaaa ccgaaagaca ataaaaattt tattcttgct gagtctggct ttcggtaagc
     2101 tagacaaaac ggacaaaata aaaattggca agggtttaaa ggtggagatt ttttgagtga
     2161 tcttctcaaa aaatactacc tgtcccttgc tgatttttaa acgagcacga gagcaaaacc
     2221 cccctttgct gaggtggcag agggcaggtt tttttgtttc ttttttctcg taaaaaaaag
     2281 aaaggtctta aaggttttat ggttttggtc ggcactgccg acagcctcgc agagcacaca
     2341 ctttatgaat ataaagtata gtgtgttata ctttacttgg aagtggttgc cggaaagagc
     2401 gaaaatgcct cacatttgtg ccacctaaaa aggagcgatt tacatatgag ttatgcagtt
     2461 tgtagaatgc aaaaagtgaa atcagctgga ctaaaaggca tgcaatttca taatcaaaga
     2521 gagcgaaaaa gtagaacgaa tgatgatatt gaccatgagc gaacacgtga aaattatgat
     2581 ttgaaaaatg ataaaaatat tgattacaac gaacgtgtca aagaaattat tgaatcacaa
     2641 aaaacaggta caagaaaaac gaggaaagat gctgttcttg taaatgagtt gctagtaaca
     2701 tctgaccgag atttttttga gcaactggat ccaggagaac aaaaacgatt ttttgaggaa
     2761 agttataaat tattttccga acgatatggc aagcaaaata ttgcttatgc aacagttcat
     2821 aatgatgagc aaacccctca catgcattta ggtgttgtgc ctatgcgtga tggaaaactg
     2881 caaggaaaaa atgtgtttaa tcgtcaagaa ctgttatggc tacaagataa attccccgag
     2941 catatgaaaa aacagggttt tgagttgaag cgtggtgaac gtggctctga ccgtaaacat
     3001 attgagacag ctaaatttaa aaaacaaact ttggaaaaag agattgattt tctagaaaaa
     3061 aatttagcag ttaaaaaaga tgaatggact gcttatagcg ataaagttaa atcagattta
     3121 gaagtaccag cgaaacgaca catgaaaagt gttgaagtgc caacgggtga aaagtccatg
     3181 tttggtttgg gaaaagaaat aatgaaaaca gaaaagaaac caaccaaaaa tgttgttata
     3241 tcggagcgtg attataaaaa cttagtgact gctgcgagag ataacgatag gttaaaacag
     3301 catgttagaa atctcatgag tactgatatg gcgagagaat ataaaaaatt aagtaaagaa
     3361 catgggcaag ttaaagaaaa atatagtggt cttgtagagc gatttaatga aaatgtaaat
     3421 gattataatg agttgcttga agaaaacaag tctttaaagt ctaaaataag cgatttaaag
     3481 cgtgatgtga gtttaatcta tgaaagcact aaggaattcc ttaaggaacg tacaggcggc
     3541 ttaaaagcct ttaaaaacgt ttttaagggg tttgtagaca aggtaaagga taaaacagca
     3601 caattccaag aaaaacacga tttagaacct aaaaagaacg aatttgaact aactcataac
     3661 cgagaggtaa aaaaagaacg aagtcgagat cagggaatga gtttataaaa taaaaaaagc
     3721 acctgaaaag gtgtcttttt ttgatggttt tgaacttgtt ctttcttatc ttgatatata
     3781 tagaaataac gtcattttta ttttagttgc tgaaaggtgc gttgaagtgt tggtatgtat
     3841 gtgttttaaa gtattgaaaa cccttaaaat tggttgcaca gaaaaacccc atctgttaaa
     3901 gttataagtg accaaacaaa taactaaata gatgggggtt tcttttaata ttatgtgtcc
     3961 taatagtagc atttattcag atgaaaaatc aagggtttta gtggacaaga caaaaagtgg
     4021 aaaagtgaga ccatggagag aaaagaaaat cgctaatgtt gattactttg aacttctgca
     4081 tattcttgaa tttaaaaagg ctgaaagagt aaaagattgt gctgaaatat tagagtataa
     4141 acaaaatcgt gaaacaggcg aaagaaagtt gtatcgagtg tggttttgta aatccaggct
     4201 ttgtccaatg tgcaactgga ggagagcaat gaaacatggc attcagtcac aaaaggttgt
     4261 tgctgaagtt attaaacaaa agccaacagt tcgttggttg tttctcacat taacagttaa
     4321 aaatgtttat gatggcgaag aattaaataa gagtttgtca gatatggctc aaagatttcg
     4381 ccgaatgatg caatataaaa aaattaataa aaatcttgtt ggttttatgc gtgcaacgga
     4441 agtgacaata aataataaag ataattctta taatcagcac atgcatgtat tggtatgtgt
     4501 ggaaccaact tattttaaga atacagaaaa ctacgtgaat caaaaacaat ggattcaatt
     4561 ttggaaaaag gcaatgaaat tagactatga tccaaatgta aaagttcaaa tgattcgacc
     4621 gaaaaataaa tataaatcgg atatacaatc ggcaattgac gaaactgcaa aatatcctgt
     4681 aaaggatacg gattttatga ccgatgatga agaaaagaat ttgaaacgtt tgtctgattt
     4741 ggaggaaggt ttacaccgta aaaggttaat ctcctatggt ggtttgttaa aagaaataca
     4801 taaaaa
//
LOCUS       DAZUMK010000161         4805 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000161 DAZUMK010000000
VERSION     DAZUMK010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.166"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..1200)
                     /gene="nagA"
                     /locus_tag="ACG808_002669"
     CDS             complement(52..1200)
                     /gene="nagA"
                     /locus_tag="ACG808_002669"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFS5048252.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1276..2112)
                     /locus_tag="ACG808_002670"
     CDS             complement(1276..2112)
                     /locus_tag="ACG808_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5048253.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2296..3513)
                     /locus_tag="ACG808_002671"
     CDS             complement(2296..3513)
                     /locus_tag="ACG808_002671"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFS5048254.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3662..3823)
                     /locus_tag="ACG808_002672"
     CDS             complement(3662..3823)
                     /locus_tag="ACG808_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048255.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3896..3979
                     /locus_tag="ACG808_002673"
     tRNA            3896..3979
                     /locus_tag="ACG808_002673"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3930..3932,aa:Leu,seq:gag)
     gene            complement(4219..>4805)
                     /locus_tag="ACG808_002674"
     CDS             complement(4219..>4805)
                     /locus_tag="ACG808_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048256.1"
                     /translation="LDMNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFG
                     NTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIV
                     GIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAV
                     KELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         1459 a         1001 c          775 g         1570 t
ORIGIN      
        1 accttaaaac caaacttaag aagtttagtt ttaaggttaa cttaaattat attatgcgtg
       61 gaaacgttcc acgccatcta gataagttgc ttccaatgtc atatcagggt tcaatacgat
      121 aaagtctgca tcgcgtcctt gagcgatttt tccacatgta tcatcgattt tacagctgat
      181 tgctggaatc agactagcca tcatgactgc ttgttctggt gttgctaatc cccagtctac
      241 aacattttta attgcttctt tcaatttcaa tatacttcca gctaagttgc cagattctag
      301 acgtgctgtt ccttctttta ctactactgg aaattcgcct aaattgtaat ttccatcagg
      361 catcccaccg gccatcatac agtctgtaat taatgcgaca tgatcatgtc ctgctttttc
      421 cattaggatt cccgcagcat ttggatgtac atgatggcca tcacaaatca attcagaaaa
      481 gacatgttct aaagataata acgcacccac catacctggt tcgcgatgat tcaatccacg
      541 cataccattg aaagcatgga caaatacact tgcgcctgct tcaacagctt tttgcgcttc
      601 ttctagtgta gcattactgt gtcctagaga aacaactact ccttcttcag tcacttgttt
      661 gacaaactct tctacacctt gacgttccgg tgctaaagca atttttttga ttaaaccacc
      721 tgaagcttct tgccattcat ggaaagtatc caaatcagga tccccgaaat aacttgggtt
      781 ttgtgcccct ttgtgttctt ctgtgaagaa aggaccttca aaatagattc ctttgatttt
      841 agctcctggt gcttcctgat acattttgcc gattgtctca gctacatctt ttaaacgttc
      901 tttgcttgac gtcaatgttg ttggtaaaaa tgaagtcact ccacaagata acagaccttc
      961 agacatcact ttgatgcctt ctgcatcatt atccattaca tcatgattca tatagccgtg
     1021 aatatgtgta tctaccaaac caggagcaat ccatttgccg ctttggtcaa tgatgttgac
     1081 ttctccctca ggtagttctt tggtgtattc accaaaaagt ccatcggtaa tttcaagata
     1141 gcctgcatgt ttgacacctg acgctaaaaa gaatttgtca gcatagataa aagttctcat
     1201 tctattctct ccctttgttt ttctacctct aaagtactct tatttagaga agaagtcaag
     1261 aatggtctac accatttaca aattttttga aatttgtttc acagcttgtg ctaagccttc
     1321 atagaatgct gctctttctg gttcttcttt acgcttgtat attaagcttt taagcacaaa
     1381 taaatcattg aggtaatagt agctattctt ctcttgagcc caatagattc cttgattcac
     1441 ctgttctaac gcttcgtcga tttcattttg atggaataag aaatttccat aattataaaa
     1501 aatttgcgaa aattcacttt ttgatcgttc atcagctgat tgatgcagca gttggatgct
     1561 tcgactcaaa tagtagcgtg cttcagccgt ttttcctgcc acaccatata tttttccgat
     1621 acaactaatc agttgtattt ctgtgtcaga aacatggatt ctttcttttt ggctagagta
     1681 gcttaatgct tctttcaaat aataaaggga ctgagagata ttttgacgca acgtgaattc
     1741 gcaaacaccg agataataat aatagcgttg aaagtcagtt gcttgataca gattttcaga
     1801 aatatcagaa gattttagta cttgttctaa cttccgataa tcacgttttt ggaaacaact
     1861 atccaacagt tcaaataatt tatcgtttgt ccgagtagtt tttcgagaga gattcatcac
     1921 atgatcaatc gttaccccta gccgttgaca aatctgcatc atcaccaata cattcggcag
     1981 ttcctcatta ttttcgatcc gacttaatac actttgtgaa caaatatcat gcgaaagcat
     2041 tttttgcgtc attttttgtt cttttcgaat ctctttaatg atcgttccaa aggactcgat
     2101 taacgtttcc ataagtatcc tccccaaaat atgcgtattc gcattctatt cgtattttac
     2161 tttaggaagt cgcaaacttc aagaaaagac ttgcaactaa ataatatact ttagttattt
     2221 aagaaaactc cactagaaag agagcctcag tgtgtttgtc tgtggccctc ctactgaaaa
     2281 atttggtttt taaaatcatt tttggcgata ggttgctagg aattctctca ttttcttagc
     2341 tgtttgtttc aaatcatcca ttttaggaag ataaacgatt cgaaaatgat ctggctggtt
     2401 ccaattaaat ccaccaccat gtacaagtag aatatgatgt tcatgtaaaa agtccaaaac
     2461 gaatttttca tcatccaaaa tattgaatcg ctttgtatca atttttggga atatataaaa
     2521 agcagctttt ggttttacgg ctgaaagacc aggaatatca ttgattgcat tatagatata
     2581 ttctctttgt tcatagatcc ttccgcctgg caggagcagc tcatcgacac tttgataacc
     2641 gcctagcgcg gtttggataa tttgctgaga caatacattg gaacacaaac gcatggagga
     2701 caacatattc agcccttcta tatagccttt gacgtgtgac ttatctccac ttaggaccat
     2761 ccagcctact cgaaatcctg ctacacgatg agatttggat agaccgttca gcgtaacaac
     2821 gaacaaatca ggcgctaatg tagcaatcgg aatatgttct agaccatcca tcactaagcg
     2881 atcatagatt tcgtcggaat aaataatcag gttattttgt cgagctactt caacgatttc
     2941 ttctaataat tcttttggat aaagtgcccc agtaggatta ttaggattaa tgataacaat
     3001 agcttttgtc cgactcgtta tttttgattt gatatcatca atatcaggat accattctgc
     3061 ctcttcatcg caaatataat gaacaggtgt gcctccagct aatgcaatag aagccgtcca
     3121 caacggatag tccggcattg gaaccagtac ttcatctcca ttgtccagca acccctgcat
     3181 acacatcgtg atcaattcgc tcactccgtt gccggtgtag atatcattga tcgtcacatt
     3241 cggaaaatgt ttcagttggc aatattgctc aattgctttt ctagcagaaa agatcccctt
     3301 ggaatctgag tagccttctg agtcacgggc attcatgatc aaatcacgaa tgacttcgtt
     3361 tggtgcttca aatccaaaag gtgcgggatt tcccgtgttc aattttaaaa tactaatgcc
     3421 ctcttcttgc atccgctccg cttcttctag aacgggaccg cgaacatcgt aactgacacc
     3481 ttctaatttg ttggattttt caaattctct catcacttag ccaatccctc atttcaaaaa
     3541 gttagaactt tccaaatttt ttgttattta ctatttcaat atcatactcc atcctagaaa
     3601 attttgccac ttatttagga aaaacgtaag tattaaaaaa acacaaacaa gcgttgagaa
     3661 cttaattgtc atgatagtca ttccatagat tcgctgccca atccattgca attccacaaa
     3721 tgattcctag cataaaacca agcaaaaaac gtccgaatgc aagtccaatc aatacaccta
     3781 agatcattcc cagtcctgaa aattctccgt catatttctc catcttaata agtcctccca
     3841 tttttgagct tgacaactat gtaaaacctc gctatcatta gtgtgttatt attacgcaga
     3901 tgtgttggaa ttggcagaca agcatgattg agggtcatgt gagcgttagc tcgtgtgggt
     3961 tcgagtccca tcatctgcac tacaactcgt gaagttaatt ttgcttcacg agtttttttg
     4021 tttgaacgtt tctttatgcc attatataca tatcactgta tcctttacaa aaagaacgta
     4081 caatcatgta aacagcctga ggtgagtata tgaaggtcgg aaacaagcta ctcgtagaaa
     4141 cttgcaagtg ttaaaacaat taatcgaaaa acgagtaaaa tccctcacct aatactaaag
     4201 cgagggattt tttgtatatt atctgtttat caccgttatt ataaaggtac aaataccaag
     4261 cacggttaat ccaaattgca taaattcatt tttttgtaca ctcatgcttt tcattagctc
     4321 tttaactgcc attagtacta agatggcgat aacaacataa agtatatagt acgtccgcat
     4381 tccggaagcc catactgtgg gagaaaatcc cataattaac cttgaacaaa atcccattat
     4441 catcaaaata actaaaaaaa tagaagtttt cttattgtca aaacttaaat aaattccaac
     4501 aattaaacat agtaataatg ccaagactac taaagtagct atccaagtac ctggataaaa
     4561 aagtgacagt ttcgttccta actctgtaaa gagattattc aggttactac tactccaaat
     4621 cattgccctt ttatttccat tgacgtaagt aaagctttga cccattgtat taccaaaata
     4681 aataatcagg tttaagaaaa acggtaatgc agtcaatata cgcacataat agttttgaca
     4741 tttcctatat gttagaagga aaattaagaa aaatagcaat aaaaataaga tattcatgtc
     4801 taaaa
//
LOCUS       DAZUMK010000162         4672 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000162 DAZUMK010000000
VERSION     DAZUMK010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.167"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..421)
                     /locus_tag="ACG808_002675"
     CDS             complement(<1..421)
                     /locus_tag="ACG808_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5048257.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQE"
     gene            653..928
                     /locus_tag="ACG808_002676"
     CDS             653..928
                     /locus_tag="ACG808_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5048258.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            1026..3023
                     /locus_tag="ACG808_002677"
     CDS             1026..3023
                     /locus_tag="ACG808_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048259.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(3099..3434)
                     /locus_tag="ACG808_002678"
     CDS             complement(3099..3434)
                     /locus_tag="ACG808_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5048260.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            3561..3776
                     /locus_tag="ACG808_002679"
     CDS             3561..3776
                     /locus_tag="ACG808_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5048261.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            3813..4175
                     /locus_tag="ACG808_002680"
     CDS             3813..4175
                     /locus_tag="ACG808_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFS5048262.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            4221..4547
                     /locus_tag="ACG808_002681"
     CDS             4221..4547
                     /locus_tag="ACG808_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048263.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
BASE COUNT         1657 a          625 c          900 g         1490 t
ORIGIN      
        1 attcttgcat ctctggttct aatttggaat atctatccag ccatgtatct aaattagagt
       61 gtactggtgg gaactgttga cgaagatgct ggaacaggtt tttatctgga aatgttatac
      121 catctagtac aatgctatct ctataaagaa taattcccaa aatatattct tctacattgc
      181 cacaaactaa ctcctctata ctcatatttc ccaaatcagc aattcgggat aatcgctctg
      241 gtattggtaa ataggttccc ttctcccatt cgtagatagt ttttttgtca attaattgac
      301 cgttgttaat cataagacca aatgctggtt gtgttaattt tttactcttt cttatagctt
      361 ttatttttgt tccgagtatc ttttttcgtt ctaaataatt tattggtgga cgttttatca
      421 tattgaccac acctcctaaa attagtatac ctcaaaaaag gggtgttgta aataaatttt
      481 acaaagatgt tgacacccct tttttatcga ggtattatgt gtatgtcgat gaactggtaa
      541 tcattttttg tttgattaat cggttaataa caaaatactt ttattttgag caaaaaatac
      601 gaagataatt cttagaaaat ttgttcaagc aatcacgaaa ggagggggaa atatgtctct
      661 acaaatcatt gatagcattg tttctataca agttgaacaa caagtgaaag agataattac
      721 agtgctaaag cctatgctag aagacattat ttctggagtc actctagagt atatggacta
      781 tcagcaggca agtgaatatc taggtgtatc agtaggaacg attagaaaat atgtttctca
      841 atacgggtta cctgttataa aaattgatac ggtcattaga tttaaaaaat ctgatattga
      901 tagtttcatg gaacggtata aaggttgagt aaacaaataa gactaaaaca tatagtaata
      961 aagagtttgt ttttattatc tgtatgtttt agtctttaac agaattttat aaggaggaaa
     1021 cacaaatgga aacatcaatg attaatattc caatttatgt agatcaagaa aaattattag
     1081 agaagccagt tacaaaaaaa gaaaaaactg atgtaagaga taggtgttta gataactata
     1141 gagaagttac aagagatgag tttgtagaga tactaaattc atcacaatca tttattccaa
     1201 gcaagacaaa gaataaaggc aatgacaagg agagttttgt ggaaaccaga gtaattattc
     1261 tggatgtcga taacactgtt aaagatgaga aggggaaggt agtagatttg agtaaagatg
     1321 atagtagata cttaagcgta gaaaaggttt tatctatcaa tttagttcat aattctgcgt
     1381 ttgcgataca aaaaagtatt cggtactctg aaaatctaga aaaatacaag attgtctttc
     1441 tattaaaaga agcaattact gactatggta aaatggttgc tgtttacgaa tacttgaaaa
     1501 agaacatccc cgggtgtgat gacaatgtaa atgcttcgaa tagaatgttt tttggaggat
     1561 ataagtcaga tgcagtgata ataattaatg aagacaacat gttagatatt actgagcttc
     1621 cgattgattt taccatgaca tgttctaaaa attgtcaaag tgattcaggg agagagtatg
     1681 aactagtcaa tgaaaccgat tttgttaagt tgataaaaaa tgacgacaaa gaagaaatga
     1741 aacagtggtt taaagactgc ctgtttgatt cctccgacat gtcttttaat gagatttatg
     1801 aaagattgtt aaccattgat atgaataaat tacttaagtc aagtaaaaac ttaagatgtc
     1861 tgtttcacaa cgatcataat ccctctgcta gtatttttac cagtaaaact gggcattcca
     1921 ttttttattg tcattccagt aattgtgata tcagtgaaaa ttttattgga gttgtgatgc
     1981 taattttaaa gaaagacagt cgggtagaaa catttaattg gctattagag aacctagact
     2041 tgtatcctac acattttcga aaattgaaag aggaatcaaa agtacttttt gacactcttg
     2101 aatcaatgaa agacaaactt ttcaggacaa taaggaatta tctagaggaa atgcgacgtg
     2161 tctatgatgt cttgctttct gatgttaatt tttttgatga tagtaaggaa agtgtgactt
     2221 gtattttgtc gggggaacaa ttagctaaaa aaatgagttc ttactatggt aaaacttatg
     2281 acatagataa atgtaataag ttactatcac ttatgacttt tataggattg ataaaaaaat
     2341 tagatgatga agatgttcct ttgaagatgc tcgaatacct aaataggtta aaggaagaga
     2401 aagcgttagg aaactttaat cataagagaa gcaatgtcta tcgattagac gtttttaatg
     2461 caacttctgt gattcaaaaa ttgaatgaag atatccttcc acttctggaa aaaattcatt
     2521 ttacatatca atatttttct tatgaatggg ttaagatatg ttttaacgaa aaagaggcta
     2581 aaagagtatt tcctcaaaat tcccgatcag aactcagcaa ggaaaaacaa ttgattttag
     2641 atgaagcaca tgaagtttta cagggcttat cgtggcaagg gacatatcta atgacagaga
     2701 aagaacttat taacactgtt tgtgaacgcc tacgtaatgt tggggataac agcgtgaaaa
     2761 atgctttaat aaaaaacaga ggctactttg ttaaacaagg attcactctt gaacgagctt
     2821 ctaaggatat taagaaacta tacaatgtga aagcaagtag tagtttttta atatatttag
     2881 cgaaaggggc agggatagat gaaacaataa aagaattgat attatcagga gctgggatag
     2941 ttcctctgta tcaatttaca agtgtaacaa gagatgatgg acggagagta aggaagagaa
     3001 aagacccaat tgtattaaac tagacaggtt atccatagct cttaatctta tttcatctca
     3061 atacacccac cgttaaagtt cggtgggtta tttatttttc aatctttcaa tagctgattg
     3121 cgttcatttt cttgcctttt taatagcttc atatagtcaa ttgttcgaac tgcatcttca
     3181 gtgctaagag aacgatagag agccaaaaac tcttcctcat aatctacgac aacattgtct
     3241 agaaaatggc ttacaggggt tcctagggct ctagaaagag ctgttaagct atcgaggtta
     3301 ggctctgtta cagaattttc ccatcttgct actgcctgtt gtgtagagaa gattttttca
     3361 gctaattctt tttgtgtcca cccgagagct tttcgctttt ctctaatgac ctttgcatat
     3421 tgtttctgtt tcattaatac acccccatct cagtatagta caacaagttg ttgtatttgt
     3481 aatggtattt gaagtttttt atattttatg agttgaaata catcatattg ttgtatataa
     3541 taaaacagga ggggagaatc atgaaaaaag taagtaatct cagccgatac agaaaagaaa
     3601 aaggactttc tcaaacggag ttagcaaaaa aaatgaatgt tacacagcaa tgtgtaagtt
     3661 cgtggcaaac agggagaacg attcctaaac cctatcaaat gaagatgctt tctgagatat
     3721 taagtgtacc aatgaatgag ctgttttctg atgtatttaa taaagtaaac agttaactaa
     3781 taagcaattg aatgatttta ggaggagaag acatgtattg tagaaaatgt ggcaaagaaa
     3841 taacagatga aagtaagtat tgccagtttt gtggagcact acaagaaaca gatgagacaa
     3901 ccggaacaga cacagtaaag aaaagtactg agcagacaat taatgaaatg aaggaacaaa
     3961 tcgtacatgg acaagagaca gggaagctgt atttgattat tggttggatt tctatggtag
     4021 tatcgttagc atttattcct gttgtatttg gtgctgttgc tgttattatg ggctacttat
     4081 atcgagaaaa agatgagaaa atgggtacga ttttgatgat agcaggggta gctggagcaa
     4141 tctttggtgt acttttaggg atgtcaatgt actgataaga gggataagag caaattattt
     4201 tggagatagg agataaaaag ttgaataaag taaatatgaa caagaagttt gtcattatag
     4261 gtattgttgt cttggctatt ataggtatta ttttttcaaa aatgacaggt acatctggat
     4321 tagagaagtc atcaaaacca ttggtaacac agattttaca agaacagtac aacttaaatc
     4381 gatcatgtga tgatgtgacg attactcatg aaaatggaga taacacatac cgggctaaag
     4441 ctatattaga caatggaagt gctatcaaca taaatattga gtactatcca aagaaagacc
     4501 gtatatatgt ggagatacca tatgctgagg tgttaatgtt aaattgattt aaatgtatta
     4561 tgcataccgt ccaaacagga actcccaaag gtgtaaattc actagggagt ttttatagtt
     4621 tcctataaat taaatactaa tacaggtgac aaaaaaatat tacaatgatt gt
//
LOCUS       DAZUMK010000163         4609 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000163 DAZUMK010000000
VERSION     DAZUMK010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.168"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(2..472)
                     /locus_tag="ACG808_002682"
     CDS             complement(2..472)
                     /locus_tag="ACG808_002682"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFS5048264.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(631..1089)
                     /locus_tag="ACG808_002683"
     CDS             complement(631..1089)
                     /locus_tag="ACG808_002683"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFS5048265.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHENYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1260..2405)
                     /locus_tag="ACG808_002684"
     CDS             complement(1260..2405)
                     /locus_tag="ACG808_002684"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFS5048266.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKRDEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2418..3335)
                     /locus_tag="ACG808_002685"
     CDS             complement(2418..3335)
                     /locus_tag="ACG808_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFS5048267.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3763..4326
                     /gene="efp"
                     /locus_tag="ACG808_002686"
     CDS             3763..4326
                     /gene="efp"
                     /locus_tag="ACG808_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFS5048268.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4463..4597
                     /locus_tag="ACG808_002687"
     CDS             4463..4597
                     /locus_tag="ACG808_002687"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048269.1"
                     /translation="MKDEWQKLEKEKTALIYEEIEMIHKIENIRTIPNYIEAKIFFHH
                     "
BASE COUNT         1442 a          843 c          873 g         1451 t
ORIGIN      
        1 gttaaagaac ggattcaatt gcagcggtca ttttcaatgg agaagtttgt ggtgcaaatc
       61 gttcgattac tttgccattt ctatcaatca aaaatttagt aaaattccat tttattgctt
      121 tagtgacagt accaggagct tcatttttaa gataagtaaa aagaggatct gtattttctc
      181 catttacgtc aatcaattga tgcataggga aggtaacgcc ataagtggtt ttacaagctt
      241 ctgctgcttc agtcgctgaa tctagctctt gtttaaattg attggaagga aagcctaaga
      301 taaccagacc ttgtgtctga taagcttggt aaagttcttc taattgttta aattgaggtg
      361 ctagaccgca ttttgttgca gtgttaacaa tgattatcgg tttgcctgca tattttttca
      421 atgaatagac agtgccgtct tctaaagtag tttcaaaatt ataaatagac ataaagtttt
      481 ctcctttcta ttttatataa taaaaagagc gctaaactga aaactagcta tttgttttcc
      541 cgtttagaac tcttttcaga caataaatat ttaataaaat acgaagataa gatagctata
      601 ttgcttctca tctttacgtt cttttagaaa ctatgcttct acttcagacc attcatcgcg
      661 ttttgctaaa aattcacgag ccaaagcttg tgcaccttct agtgtatggc tggcagccca
      721 gccacattgt gtttcattgc ttgcaggtac ttcagtagct tcaagaacat ctttcattgt
      781 tgcttcaaga acatctaaaa tgtcttcgta gttttcatga ttcaatacag tcaagtaaaa
      841 gcctgtttgg catcccattg gagaccagtc tacgatgtaa tctgcgtgat tgcggattag
      901 ttcggcagtc aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt ctttatttgg
      961 ttgtttaaag cggacgtcgt atttaatgat gacatcgcca tgcgcacccg tttttcgatc
     1021 agctacacgt acataaggtg ctttgacttt tgtatggtca agattaaaac tttcgacatt
     1081 cattctcatt tgtaagactc ctatctgttt gttcattcgt tttgatgctt ttcactaaag
     1141 aattatttag tgaaatttac aaaaacgtct ttctacttga ttctaacact tatcgtttcc
     1201 aaggtgtagc aatctgaaat attttttaga tttttctgct aagtcgttta ttgggacttc
     1261 tagttcttga ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg accatactca
     1321 attcctacag aaagacggat caagccgtcc tttatgccag cctcatctcg tttttctttt
     1381 ggaatagacg catgagtcat gacagaagga atttccacta aactttctac gccacctaaa
     1441 ctttctgcta aagtaaatag ttggagtgat tcaacaaatg gaatagcaga ttcttcattt
     1501 tttaaagtaa aagaaatcat tccgctaaag ccgctcatct gctgcttagc aactccatgg
     1561 agttcatgat cgggtaatcc tggatagtag accttttcta ctgctggatg agaaaataga
     1621 aaatcagcta ccacaaaagc atttttttga tgttcctcca tacgaacact tagtgtcttg
     1681 attccgcgct gaagtaacca gctatcttgg ggaccaagta cggctccgat agcattttgg
     1741 taaaatccga tctgatcggc tagatcttta tggttagaag tcactaaacc agctactaca
     1801 tcgctgtgtc cgcctagata tttggtaccg ctatggacaa cgatatcaga accgagatct
     1861 aaaggtcttt gaaaataagg agtagcaaat gtattgtcag caataactat caatccatgt
     1921 tgatgagcca gagtggcact tttttctaaa tcagtaatct ttagaagcgg attgctaggt
     1981 gtttcaagat aaagtgcttt tgtattgggt ttgatggatt gttctatttt atcaagatta
     2041 ctcgtatcta tgatcgtgta ctcgagacca tttttagtaa gaactttgtc aaatagacgg
     2101 aatgttcctc catagacatc atctcctaat aagatatgat cgccagcttg aaaaagagaa
     2161 aaaacggcat gaatcccaga aagaccagaa gaaaaagcga atcctcgaac accgccttcc
     2221 aaatcagcaa tcagttcttc caatgcaaaa cgagtaggat taccagatct tgaatattca
     2281 tattgtttag gctggccgac gccatcttgc cgataagtgg atgtttgata aataggtaca
     2341 ctgacagctc ctgttgtggg atcttcactg atgcctccgt ggatcaattt tgtttgaatg
     2401 tgcatgtttc tttcctccta taagtaaatg ttttttgata aatagcggtc tgctgcgtct
     2461 ggaaaaatcg taacgacacg atgaccaggt ggtaaacgac gaatttctct taatgctgct
     2521 gcaaaagccg caccactcga acttccaact aatagtcctt gttctcgtgc taactgccta
     2581 gtatagttaa acccttctac atcagagatt gtctcgatct gattgattga aaggggagaa
     2641 agaaacggcg gaatgaattc tacaccgata ccctcaattt cgtgaggggc tggatcacca
     2701 ccatttaaca cagagccctc tggttctaca ccgattattc gaatgtcggg atatttttct
     2761 ttcaaaaagg tagaagttcc agcaaaggtt ccaccactcc caattcctgc tacaaaacta
     2821 tggatatttt ctttgatttc atgaaagatt tctggcccta acgtatgata gtaagcagca
     2881 ggattatctt tattttcaaa ttgaagaggc agatagctgt ttgatatctc ctctgcaagt
     2941 tttttacttt tgctgatggc accgatgatt ccttgctcac taggagaatg gatgatcttt
     3001 gctcccaatg ctttcatcaa ttgttgtttt tctaagctaa acttctctgg aacaacaaaa
     3061 accgttctta atttgtatgt gagtgcggcc agagcgatcc caattcctgt atttccggca
     3121 gttggttcga tgatcgtcgt tttttcattg atttttcctt gggcaaatcc ttctttgatc
     3181 aaataactgc ccaatctatc tttgatactt cctccaggat ttaaatattc tagttttgca
     3241 taaatagcag aacctttagg catagaatag tgctgattgt caaagcgata aagcggtgtg
     3301 gaaccaatag cttctgtaat tgtcgagata atcatgtggg cctcctaaaa aaagataata
     3361 aaaaaaggag ctgactatta aatcagctcc ttgaaagaca tagagaaaaa ttgagtagat
     3421 aaaaagataa gttacctggt actcattcta tgaagggcaa ttaatagaca tgacaaatag
     3481 tatgctgctt ttatgcacca tacaacagca acgattcaat tgtttttggt tgctgactgt
     3541 catgtgtatc cctccaagta aaatttttat taacgatagc atgaaaaaaa ataaaagtca
     3601 aaggcttgaa agaaaaaaat atttcgatag catcgtttac ccgtgtttat ttttctgaca
     3661 aataggcatg agaccttaaa agatagagaa tactctagtt ttctggccgg ttgtctgata
     3721 caataaagtt gattatgata aagaaaagaa ggagcaaaaa taatgatctc agcaagtgat
     3781 ttaagagccg gtatgacttt cgtacaagac ggcaaactga ttaaagttct ggatgcaagc
     3841 caccataaac ctggtaaagg aaatacagtt atgcgtatga aactgaaaga tgtccgttca
     3901 ggtgcaacat acgacacaac gttcagacca gaagaaaaat ttgaaaaagc acatatcgac
     3961 acaaaacctg tacaatattt gtacacgatg gatgatacag ctttcttcat ggatttggaa
     4021 acatatgagc aatatgaaat tcctgtagaa acagtagaag aagaaatgaa atatatccta
     4081 gaaaacatgg aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt tcaattacca
     4141 acaactgtcg tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc aacagtcact
     4201 ggttcaggta agccagcgac aatggaaact ggcttagtag tcaatgttcc tgacttcatc
     4261 gaaacagatg aattgttgga aatcaataca gcagaaggtt cttatgtaaa acgtgcgtca
     4321 aaataattcg ctaacggatt gataagaatt tgaaaacaga gcttgacctc ttgaaaggca
     4381 gatcatcgga tctgcctttc aagaggtttt ttgtggaatc agcaaattga aaaataaaaa
     4441 agtatttgat tttatttata ttttgaaaga tgaatggcaa aagctagaga aagaaaaaac
     4501 tgcacttatt tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc gaactattcc
     4561 gaactatatt gaagcgaaaa tattttttca tcactaaata gtgttgaat
//
LOCUS       DAZUMK010000164         4561 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000164 DAZUMK010000000
VERSION     DAZUMK010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.169"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..587)
                     /locus_tag="ACG808_002688"
     CDS             complement(<1..587)
                     /locus_tag="ACG808_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048270.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVF"
     gene            complement(735..1277)
                     /locus_tag="ACG808_002689"
     CDS             complement(735..1277)
                     /locus_tag="ACG808_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFS5048271.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            1412..1801
                     /locus_tag="ACG808_002690"
     CDS             1412..1801
                     /locus_tag="ACG808_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048272.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1805..2569
                     /locus_tag="ACG808_002691"
     CDS             1805..2569
                     /locus_tag="ACG808_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS5048273.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2698..3645
                     /locus_tag="ACG808_002692"
     CDS             2698..3645
                     /locus_tag="ACG808_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5048274.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3775..4515
                     /locus_tag="ACG808_002693"
     CDS             3775..4515
                     /locus_tag="ACG808_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048275.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         1591 a          756 c          921 g         1293 t
ORIGIN      
        1 ataaatacac ctagactgac gatgaagagc atatccaaca gatttcctgc gacttggatc
       61 gtcgaagggt tggtcagtgg cattaaagca aggacaaaaa aggaaaacaa gctgtaaata
      121 aaacaggtga caggaactaa tcgaaagaag agcggacatt tcaattggct aaaaatgagt
      181 gaaaaaataa aataagtaaa aatggataaa atcacgacta aaccattcat gataaagaag
      241 aaaagcgtat tgtgattaaa cgaataaaaa agaattgcta aaaaagaaaa cgttttaaat
      301 gcaatcaata cgcttgaagc ttcgtttaac gtaccaggat gtcgatgaaa aaaataggca
      361 ccgaaataaa cagaaaacag taagccgcaa agagaagaca aaatcaaaaa aaacggattt
      421 tcttcggctt ctgtgatatc agaatgatcc aaaagcaacc agcctaatgc actagaaggc
      481 gcatgtgagg tcttaaggta tgaataacat agaaaaaata agatcgtcaa agttgcaaaa
      541 aaagagccta aatgtaaaaa attagtggtt ctacgttgaa tttccataat atctccaaat
      601 taaattgata agtatataaa gtataataac aaattgatga gtatatgtcg atgtttttta
      661 atcaaaataa taaataaaaa ctgtctaaca tttaaaattt aatttagata ttagacagtt
      721 tttaagtttg tgagttattt tccttgtcgt tttctcactt ttttagtcca gaaaccacct
      781 gaaatatatt ttggctcata agaaatgata aaagcacgct cttcgatttc tttgatcagg
      841 cgatacagtt cacgttcgct ttttctagga gaaagtattt cgagaactag ccgctcacct
      901 tccagcccca ttgcagaagt ctgtgtcaca ccataaccgt gttctctcag tatacccggc
      961 aaacttttat cttctgcgct atttgctggc aaaatcgttg tgaccatgat gtatcctaat
     1021 gccaatttgt cttcaatcaa aattccgaca ctgataccta ctgcgtaacc taacgcatag
     1081 acaaataaat tcagtggatt atctaaacga tctaaaacaa gggataagcc gacgatataa
     1141 atcgtgatct cgaccatact gacaagtgga gcaatcagtc gatagccttt cattgtcaac
     1201 atgaaacgta acgtgttcag agtgatataa gcaaaattaa tgaggaagat catccccaac
     1261 atttttggat caatcatttg taaaactcct ttcatttata taacaacaat atcaaaaaaa
     1321 atcgtaagtg aagaaaaatt gaaactttat acatacaaaa aaatgataaa atgattataa
     1381 tagaattata aagataggtt aggtgattaa aatgaaaacg gtcaatcgtg tgttttgtgc
     1441 cagccacgtg ctaagtctcc ttctcagtat atacttaggt gtctattcga ttcaacagcc
     1501 aattgaggta gagaacattg tttttcagat gtcgctgaca gatggtttaa ttttaagcgt
     1561 ttttttcttt ttgatactgt ttattggtaa tattttcggt agcgttgcca gttttttgaa
     1621 tttttctatt tatccactcc tttcgctagc attaggagtg gtgggacttt gttctctgtg
     1681 cttatttccc gagccattca gtcctgcatt tatattattt ggtacactga acctgttcca
     1741 gacaactgtt ggagcatggt tgctatggcg atcaggaaat ttaatgaaaa taggtgagta
     1801 acaattgaat ccaattatat tgaaacgttc tgtccatcat attccagtgt tagaagttgt
     1861 tctgaaagaa ttaagagagg aacgtattcc cgttgtcatc tattatcacg gatggcagac
     1921 aagtaaggag ttagttttga cgcaaggaag aaaaattgcg cagcagggca ttcgtgtgat
     1981 cctgccagat gcgatgaatc atggggagcg caaacagccg gtttcttcta ttccatccta
     2041 tacattttgg gatagtatct acggaaatct gtttgaattt gacacactta tcaagcattt
     2101 agaaaaaaga ggtcttctac atgatcagct tgcagttggc ggggtttcga tggggggaat
     2161 gacaacatgc gccttgttgg ctaaacatcc tcatatagta gcgggcgtgt gtttgatggg
     2221 ttcccctgca cctttggaat atggaaaaga aatcgtaagg cgcgcatcgg aatataatta
     2281 tgtcgtacca aaagactatt ttgatttgat ttcatgggtt aagagctatg atttgtcatt
     2341 gcagccggaa aagttagctg gacgtcctct gttcttttgg catggacaag ctgacgaaaa
     2401 aatcccttat atacaaacgg aacgatttgt agaaaaaaat cggcataagt cttacggaaa
     2461 agaaattgtt tttcgttcct caaaaacaca gcgacatatg gtacaaatac cactaatgga
     2521 agaagcttcc acttttttag caaaaaattt gataaaaaag tataaataat tgaaacggaa
     2581 aatcactcat gacgatagag gattttccgt tttttatttt gggctataat aaactctgtt
     2641 aggaaaattt ggtataattg atagtaagaa tgacaacatg ttagaaagga agagagcatg
     2701 cccactttag cagatgttgc aaaacgtgcc aatgtttcaa aaatgacggt ctctcgagtc
     2761 atcaatcatc cggaacaggt gactgacgaa ttgaaagagt tagttttctc agcaatggct
     2821 gaactggatt accgtccaaa tattgctgca aaagcacttg tcacgaatcg gtcgcagatc
     2881 atcaaattat ttatactgga agaaatcgac acaactgagc cctattacat gaatttgctg
     2941 atgggaatcg ctaaacggat cggtaaagcg cattattctc tccaattagt tacaaatgat
     3001 gccttcgacg tcggttcttg tgatggatac atcatcacag gtgtcagaaa aaaagacttc
     3061 gattggatcg accgtttgga aaaaccagtt gtcttgtttg gggaaaattc tgaaggatat
     3121 gattatgtag acagcgataa tgaatatggt acagcaaaag caacgcaata tgccttagaa
     3181 agagattatc aaacgattat ttatatcggt atcgaagtgg acgagccttt tgaaatttca
     3241 agggaaactg gttatttatc tgtgatgaag gaagttgatc ttcctagtaa aatcttgcgg
     3301 tttccaaatc attcgacaga agtagaaaga tatatgacag aaaactgggc agattttaca
     3361 gataaaactt gttttgtatg tagttcagat cggctagcac taggagcagt aagagggatt
     3421 ataaatgctg gaggacactt gcccgaagaa tttggtgtga ttggatttga tggggtattt
     3481 ttagaccaaa tcagttcacc taaattgaca acgatcaagc aagatattat acgtatgggt
     3541 gaagtatgtg gagaaatgtt gttaaaaaaa atagaagaaa aaggggcgag tcaaggttat
     3601 cgtcgttttt ttcctgaact agttatcaga gagacaacga attaagtctt attactatat
     3661 atatatttat gaaattcatc gctattttct taaaataatc agcgcattgc tactgaaaac
     3721 aatgaatctg tgctattatt gagaaaagtt ccatttttga gaaggatggt ttgaatgaaa
     3781 gaaatcagac gaaaacattt ccgttttcct gcgtacgatg atgagttagg cgtaaaatta
     3841 accaacgaca agaagcgagt attatttgac ggacaagaag atcttttgac tgaacagaat
     3901 cagtccccgt ttgaacagat gaaagaattt catttcacga acacgaacac cgagaaaatc
     3961 cgtcgcaagc gtcgtgcaac aaatggattg aagtcagctc cgcgtaagga aaatttggaa
     4021 aagcataaag gaaaattacc ggattatacc ggccattacc aatcagaaat cacatcgaca
     4081 ggacgaaaaa aactttttgg tgattcctct cgttctgtca gacaaagtga aacaaggaca
     4141 gagtccaagc atactacacg aaacgcatct gttgacacga aggtgcggga gcgttcttat
     4201 tttgttccta aatatgtacc cgcatcgatt atcccagacc ctagagaaag caaaatttct
     4261 gaggatcatt taatcgaatc gatgaaaaaa acggatgact cttatctgtt gttcgataca
     4321 gaaccgacgc cgtatcaaga aaaaagaaat ggtgagccgt ctgtacgaac attcaatcga
     4381 acaaaaacgt tagaggaaac aaagcagtca gaagaaaaaa aatcgaaaaa aagccatggt
     4441 atcttagaac gctcattaca aggattgatc gatgatcaag gaaactcttt gggagaaaat
     4501 agctatttta aataagtgat atacaaattt atattcggct acagttggaa aggaaacgct
     4561 g
//
LOCUS       DAZUMK010000165         4530 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000165 DAZUMK010000000
VERSION     DAZUMK010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.170"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            41..1981
                     /locus_tag="ACG808_002694"
     CDS             41..1981
                     /locus_tag="ACG808_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFS5048276.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
     gene            2068..3990
                     /locus_tag="ACG808_002695"
     CDS             2068..3990
                     /locus_tag="ACG808_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS5048277.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLLSSYE"
     gene            complement(4270..>4530)
                     /locus_tag="ACG808_002696"
     CDS             complement(4270..>4530)
                     /locus_tag="ACG808_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HFS5048278.1"
                     /translation="GIDISKQTSDLIDRDYFTQADLIITLCGDAKDKCPAVPNNHEHL
                     HWDLKDPAQAVGSEEEILTEFRKVREGIKRKVLDLNQRTSIV"
BASE COUNT         1496 a          774 c          937 g         1323 t
ORIGIN      
        1 ccgtttttat atactataaa tgtagaaagg cggtaggaaa atgaaaaaaa ttattgcaat
       61 cacatcctgt ccagttggta ttgcccatac atacatggca gcagaaaact tagagaaagc
      121 ggggaaagca gttggcgccg aggtaaaagt cgaaacacat ggttccatcg gtattgaaaa
      181 tgaattgact gcaagagata tcgaagaagc agctggtgta atcatcgcag cagatactaa
      241 aatagataaa tcacgattcg gcggtaaacc tttgataacc gtaggtgtgc aagaaggaat
      301 ccaccatgca gatgagttgg tgagagatat tttagcagga aaagcgccag tctataaaag
      361 tacggaggca attatttctt ccgaaaataa aagtgaatca gaaaatttag gtaaaaaaat
      421 ctataagagt ttgatgaatg gcgtttcgta catggtacct ttcgtagtta caggagggtt
      481 attgatcgcg atttctttaa cattaggagg aacagctaca cctgaaggga tcaagatacc
      541 agaaggaacg atttgggcaa cgatgaatag tatcggaagt atcgctatgg ggttgatggt
      601 tccaattcta tctgcattta ttgcgcaaag tattgcagat cgtccaggac ttgttcctgg
      661 atttgttggt gggatgttag cggcaaacgg agcattatat ggtagtgatg cgaatgcggg
      721 attcttaggc gggatcatca ctggattttt agcaggattc attgctttag gaatcaagaa
      781 agtgcgagta ccgaaagctt tacaatcgat catgccaatt attgttatcc caattttagg
      841 atcattggca gttggttttg tgtttatcta tgttattgga gaaccagtag ctcttctatt
      901 tagttcattg actcactttt tagcagggat gcaaggagga agtgaaattg ttctagctat
      961 gatcttagga gcgatgattg cttttgatat gggggggccg ttcaataaag tagccttttt
     1021 gttcggatct ggcttgattg cagaaggcaa ttattccatt atggggccaa ttgctgtagc
     1081 tatctgtatc cctccattag cattggggct ggcatcattg attttaaaaa ccaaatttac
     1141 gaaaacagaa cgagaagctg gtaaggcgtc cttagcaatg ggactgtttg ggattacaga
     1201 aggcgctatt ccgtttgctt caacagatcc actacgggtg attccaagca ttgtaatcgg
     1261 ggcaatgaca ggaagtgtga tcgcgatgct ttcaggagtg acagatcatg tagctcatgg
     1321 cggtccgatc gtagcggttt taggagcagt ggatcacgta gccatgtttt ttgtagcagt
     1381 gattgctggt attgctgtaa ccgtgctaat gttaagaatc cttaaaccga cattacaaca
     1441 aactgcggtt gctgcagcaa atggtgtgac agtagcagat ctatcagaga ctgaaacaag
     1501 tgatgtttat acgaaaactg aaacttttga ttctatcacg gatattatgt cagatgaagc
     1561 aatcgtttta ggtgaaacag caacaacaaa agacggagta atcgaagatt tagtccaacg
     1621 tttagaaaaa acagcggcga tcacagataa aaccagattt aaagaagtca tctatgatag
     1681 agaacatgaa agtacaacgg gaatcggtat ggggattgct attccacatg gaaaatcagc
     1741 ttatgttctt aaaccaacag tggcatttgc cagaagtcaa tctggggtag attggcattc
     1801 tttagatgga aaacctgcac atatgatctt tatgattgct gtacctgaaa acagccaagg
     1861 tgatatgcac ttgaaaatac tgcaacgtct atcacgtaaa ttgatggatg atgacttccg
     1921 tcaagcactg atggaagcgc cagataaaac aacagttcac caattattaa gcgaaatgta
     1981 acataagatg aaaaatcgac cgtgatgaga cataatagat gtgttatcat gatcgatttt
     2041 ttatttagaa gggagtttct gcacattatg aatcagcgtc agcaatcttt gatctatcaa
     2101 ttaagtgaga acaataatta tataacaaca aaaaagctag cagagcattt ccatgtgtca
     2161 gaaagaacga ttcatagcga tttgaatacc attgaaactt ggttgagaga caaacatctg
     2221 cctttaatgt ttgaaagaaa aaaaggtaca ggtatcttgt tgaatggtct gcctcacgaa
     2281 agggaacaac tgaaacgagc gcttaatgaa gagactcaaa cagaaatgga taaaaaacag
     2341 cttcgtcagt tattgatttt ccatctgcta gtagcaaaag acggatttag tcttgaagaa
     2401 ctttcagaaa aactatatgt tggaaaacgt acgattcgta aagaattgac agagttgcaa
     2461 agcttttttg agtatcacca tttacagcta gtatccaaaa caaaactagg aacttttctt
     2521 aaaggcgatg aacaagaaaa aagacagttg cttgttaaga cattacgaaa tatgcagaaa
     2581 gaagatccac aaagtccgtc gttgaaagaa ttcttcgcta aagatacatt aaaagttatc
     2641 caacatacat tacaagatgt tttttatgaa aaccatttag aacagccgaa tggactggct
     2701 agtgtggaga ttcacatcta ctttatgctc gagcgaatga aacagtacca gaaagtgaaa
     2761 ttaagtaaag acgaaaacga agtcgtggaa cacacccaag cacaaaagtt aagtagtcaa
     2821 atattagcaa aactagccac catctatcca attgaatttt ctcctgatga aatcaattat
     2881 ctggctttgc gtatcgccaa tctttttaca aattcacaag cagccacacg ttttcaaaag
     2941 gaatctagcg cacttgctga tcatttgatc gaacaagtgg aacagtttct aggctattca
     3001 ttaaaagaag atcagctact aaaacaaaat ttaaggtctc atttatcttc aacttatttt
     3061 cgactccatt atggtttgca gatttctaat ccattgacga aaaatgtgtt tagtacgtac
     3121 acacaacttt tcttagtttt acagttaatt ttagaagatt attttgaaaa ggaaaatttt
     3181 tatgttccac aagaggagac agcttattta acgatccatt tccaagcagc gatcgagcga
     3241 cacaaacatc agaaaagtag aagatatcaa acggttttag ttagtgaata ttcaaaagct
     3301 atggctactt ttctcgaagc acggctgaac agagaacttc cagaattaac gattatggat
     3361 ctagttgaat ataagccaga attaaagaga caagattttc cctctgttga ttttatcttg
     3421 gctacgttac catttaaaca tataagcatt ccagtaatcg agatctcacc aatgatcaca
     3481 gaaactgatc tagcctattt gacaaaatat atgctggagc atgtgcctat caagaagaaa
     3541 aaaacatttg atctagcaag ctttactcat ccatttctga tctttcctca acttgaacgg
     3601 acagacccag ccgatatttt aaactttatg ggaaacgtct tagtggaaca tcattatgtg
     3661 gagccggaat tcatcgattc tgtcttggaa agagacagtc actccagtac tagagtagca
     3721 ccatttgtaa caatccctca cggtaatccg ctgtatgtga agcattcgat gatttctatt
     3781 gctacaatga aagaaccagt cctatggcac ggggaacaga tacgtatcgt actgctatta
     3841 gcaataaaga aaaatgattt gaaacatgca gagttcaaaa aaatattttc tgttattcac
     3901 tatctagaaa agcagccaga acaaatggct cagttgatgc aatccaatca tccgctagaa
     3961 atcttaacgt tgttatctag ttatgaataa gtagcgatta aaaaactgcc tttatattta
     4021 taataattct atctatttat ttggaagtag aaaactaaat acaaaaaaag aggatgtggc
     4081 caaagttgtg gcacatcctt ttattttgag tttcttataa ttttttatag ttgcttacga
     4141 tgtcttgact actggtaaac ggtgttcaag agacaatttg tcggcaataa ggcacaattt
     4201 tgtaaaatat ggaaagctat ttttttaaca ttgtttctta gtgcttgaaa aatcattttg
     4261 aatacttctt tatacgatcg atgtacgttg atttaagtct aaaacttttc ttttgattcc
     4321 ttctctgact tttctgaatt ccgttaatat ttcttcttcg ctcccgacag cttgtgcagg
     4381 atcttttagg tcccagtgaa ggtgttcgtg attgtttgga acggcagggc acttgtcttt
     4441 ggcatcacca caaagtgtaa tgatcaagtc tgcctgagta aagtaatctc tatcaatcaa
     4501 atcagacgtc tgtttggaaa tatcgatgcc
//
LOCUS       DAZUMK010000166         4488 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000166 DAZUMK010000000
VERSION     DAZUMK010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.171"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..1085
                     /locus_tag="ACG808_002697"
     CDS             3..1085
                     /locus_tag="ACG808_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015076493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5048279.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1303..2445)
                     /locus_tag="ACG808_002698"
     CDS             complement(1303..2445)
                     /locus_tag="ACG808_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5048280.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2450..2635)
                     /locus_tag="ACG808_002699"
     CDS             complement(2450..2635)
                     /locus_tag="ACG808_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFS5048281.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(2989..3234)
                     /locus_tag="ACG808_002700"
     CDS             complement(2989..3234)
                     /locus_tag="ACG808_002700"
                     /inference="COORDINATES: protein motif:HMM:NF024059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5048282.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKNN"
     gene            complement(3228..3608)
                     /locus_tag="ACG808_002701"
     CDS             complement(3228..3608)
                     /locus_tag="ACG808_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFS5048283.1"
                     /translation="MSKQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLADSNEGRY
                     SQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDAEIGKILN
                     MSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(3976..4317)
                     /locus_tag="ACG808_002702"
     CDS             complement(3976..4317)
                     /locus_tag="ACG808_002702"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048284.1"
                     /translation="MSIDDCLEEQIRLFLKFLKVKVQNNYFTCYSYAHPVLNSLPFNS
                     LVQGLITNIVLLVCTPNEIFVKKIGSGISSVRLKEKEYTQKIIHIPKPEIKRFEIKDY
                     SALGLFSIGYW"
BASE COUNT         1513 a          796 c          650 g         1529 t
ORIGIN      
        1 atatcaatga tgatggctca agaaaaaaag tggagaaagt tggcggagac acccgaccag
       61 aggccgaagc tgctttacga aaagttttat cagatattga cgaaacagga caatactttt
      121 taggtacgga tactcgagta aaacaatacc ttgatttttg gatggaggaa tacgttaaac
      181 taaatctaaa atacaatacc tatgaaaact accgatttac catcaaaaat catataaacg
      241 gttatttagg aaagaaaaaa cttacggatc tctcccctgc tcttttacaa aatttcatca
      301 atgctgaatt taaaaagggt tactcgaaga aaacaatgac tattactcac tctgtcctta
      361 agaatgcgct gaatatggcg gtttatcctt ggggattaat caagcaaaat cctatgctgt
      421 atgtaaaaat accaaaatat gaagcacgac caacgactaa aaaagatcta aaaatcattt
      481 ctcttgagga ctttgatcat atgctagaaa ttactcctga aggacatcct ttctatattc
      541 ctttgaatat tggattttat acgggaatgc gcgttggcga agtttgtggt ctgacgtggg
      601 atgatgtcga tttttcaaat ggaacaatta ctgtagagaa acaaatggta aagaatgacg
      661 gcgcatgggt atatggtacg ccaaagacaa gcagttccaa tcgaacgatt tttattggac
      721 aaaccttgct agcaattctg aaaaaacata agaaacaaca attagaaaat cgaatgaagt
      781 atggaaagct ctacattgat tcaaatgcag tatgtacgaa ggaagacggt gagctagtta
      841 cgccaagtgt agtgaaatgg aatactagga ggatatcgaa tgcactctcc ctctctttta
      901 acttccattc tctcagacat actcatgcta cacttcttct cgaaaatggc gcaaaaatga
      961 aagaaatctc tgaacgattg ggccacagca gaatttcaat tacgatggat acttactcgc
     1021 atgtaacaga taagatgaga aatgaaacgg tcgatatcat ggaaaatctt agaaagaatt
     1081 cttgattttt gccaccgaaa aaaaccaccg gtggcaaacc ggtggcaaat ccatgtaaac
     1141 atggtttatt ttttacactg ttttcaaaca aacccttgct atttcttcgg ttcgtcctcg
     1201 tccattttcg ataccgacag ctagcttata acccaaggta tatcaacata aaaaaataaa
     1261 aataggctat gacagcagcg tgacagcaaa aaagatggaa agttaaatat caaacctcgt
     1321 aatttcattt gcgagtgctt gatttgtttc tggatataag tgtccatatt tgtccaaagt
     1381 agttttaata gaggcatgac ctagacgctt agatattgca tagatatttt tattatgttc
     1441 aattaaaaag gcaacatgcg agtgtcttaa tccatgcata tgaattggtt ttactcctgc
     1501 aatttcagct agttttttta ttctgcttaa aatagttctt ggagatggcg gtaatccatc
     1561 aaaagaaaaa accaattcta tatttttccc aattgattgt tgagtttttt tccatgtttt
     1621 taaatcttca acggtttgtc tgtctaacga aattatccga atacttgatg tattttttgt
     1681 ttcagcaaat tcataatctt gtcttgtacg gatgtaaatt gacttattta cgctacatct
     1741 gccatttatt aagtcaaagt ctttccattg taacgctaag agctcctcac tccttacacc
     1801 tgtaacaaaa ataaaacgca tcaaacgttt ataaaattct tcataaaaat ccccgtgata
     1861 aacactatta tatactttct taaattcatc aactgtccaa aattctactt gaggacgctg
     1921 agtttttatt ttacctatag tatctacagg attttcttca ataatttcaa gtacaattgc
     1981 ccttttaaaa actactctca acttattgaa tatctgttta atataatttg gtgataatgg
     2041 ttgctcaatg ccctttttta caacatggat tttcgataaa aactgttgaa attcctgtat
     2101 atgaattggt cgaatcttta caacctgcat ttctccaaaa aaatccaatg cacgttgtaa
     2161 aatatgatct gttttcatat atgttttttc tacagttccc aacttatacc aaggaaaaaa
     2221 atactctcga taaaaatcat gaaacgttat tccttgcaga taaattcctt gtgaatcaga
     2281 aaaactttgt tttaactctt cgagtcccct tctagcttct ccttgagttt tgaagcctct
     2341 tctggtcact tgaattcttt ttccaaagcg atcttgtcct aaagttgcag aaaaaaacca
     2401 agtgcctctt tttctgtctt tataaacatt cgcaattttc ctcattttac tattcctccc
     2461 aagaaatatg gagaacttct tctaaaattg aaataggaat tctttttatt tttgaattac
     2521 gataaaaaaa gtatcctttc tcaactaaaa gttctttaca ttccttgaag attttattag
     2581 catagtactg agaaaaacct agattaatca aatcttttcg agtagctgtt ttcattatag
     2641 caccttcctt actttttttc atttatttct atcagctgct tttgtcaaaa gcacatagga
     2701 atttcacctc tccccaagtt tacggcgagc cttacggaag tatcattatc ccaactttaa
     2761 atcatcgctt tttattgact ataaaaatta ttttagaata tttatcgctc cttctttaca
     2821 gcaagaaatc atggcgcaac taaaatatgc tccttaaagt gttaacgtac tgatagaatc
     2881 tatttaattt tcaatgaaca ataggttttc tacctaaaag aatctactaa ttccttaact
     2941 attagaagat tcttttaaac agaaaaagtg gaaaggggat ttttttaatt agttattttt
     3001 aaattttaag atcgcttcta ttaatttaat ttctagctga cttatcatat attcatctac
     3061 ataggtaacg aattgaccta cctcgttaaa tccatttgtc aaacataact tagccatata
     3121 agaatgataa tgtttgagta tcatcttgat cgaaattgga tctccttcta gagcagataa
     3181 gataatggat agtggaacta ataggtactg ccgcttttta gatttatcta ccataggtca
     3241 taccccattt tcaattgttc aactgccttc tttcgttgat accagatacc acttgtgctc
     3301 atattgagaa ttttaccgat ttcagcatca ttaaaacctg caaaataata aagtaggata
     3361 atctttcttc ttatctccga taactgatca attgctgctg ataacttttg atctgaaatt
     3421 aatatttcca ttccaaataa gaaatagaca tcttgttcat caaaatgagt ttgggaatac
     3481 ctgccttcat ttgaatctgc caatggttcc tcattatcct ttatgttagc gtttcgtttt
     3541 cgaatagact tagcttcgtt tcgtaatact tttttacagt aggcatcaaa ttggtgttgt
     3601 ttagatattt agttgtagtt ttgtgacatg ataagtatgc tcctttttct cttttccact
     3661 aatacaacga tcaaagggtg cttttttcaa aaaactagaa aaatcaaaat aagattcgta
     3721 ttttgattct atatatcctg tttcaactac atcttatatt tcacaataga ataaatagcc
     3781 ttttgcatag ctgcttaact tataagaaat tgcaatttac ttcttcaaag tgaaaacgaa
     3841 tatgagtaaa ataaaattat tcttcttaca gataattaaa aatagcgcgt aacaaaaatc
     3901 ggcattcaat cacataatta ggattgatgc cgattactta tattctacgt atagttttag
     3961 tattcttatt ttttatcacc agtaaccaat tgagaacagc cctaaagcag aatagtcttt
     4021 tatctcaaaa cgcttaattt caggcttagg aatatgaata atcttttgag tatattcttt
     4081 ctcttttaat cttactgaag aaataccact gcctatcttt tttacaaata tttcatttgg
     4141 tgtacagact aataaaacaa tatttgtaat caatccttgc actaaagaat taaaaggaag
     4201 actatttaaa acagggtgtg cataactata acatgtgaag tagttatttt gaactttaac
     4261 ttttaaaaac tttaaaaata atcttatttg ttcttctaaa caatcatcta tactcatata
     4321 atcactccac taattaatct ccccaaaaag acacagcatt aagagatata ccaacacaac
     4381 agtgcaaata actctacatc ttccttcgca accttatttc aataaaccat aaaaattatt
     4441 ttttaataga aactgaaaat taaaactaga aaaagtagtt ttaccgaa
//
LOCUS       DAZUMK010000167         4464 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000167 DAZUMK010000000
VERSION     DAZUMK010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.172"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            573..1007
                     /gene="bacA"
                     /locus_tag="ACG808_002703"
     CDS             573..1007
                     /gene="bacA"
                     /locus_tag="ACG808_002703"
                     /inference="COORDINATES: protein motif:HMM:NF041453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 51 precursor BacA"
                     /protein_id="HFS5048285.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2297..3220
                     /locus_tag="ACG808_002704"
     CDS             2297..3220
                     /locus_tag="ACG808_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS5048286.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3236..3775
                     /locus_tag="ACG808_002705"
     CDS             3236..3775
                     /locus_tag="ACG808_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFS5048287.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1667 a          667 c          756 g         1374 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacggagaa cttaaattaa attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagagacg gataaataat aaaaaagact
      181 gctctttcga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcacgcgctt
      241 atgaagcgct accaaaaatt taccatattt tacaaaaaat aacaagtctt aaaaaaatga
      301 cagataaaaa tgcacaaaga attagatata gataaccgcg aaaaatcaag ctttagaaag
      361 ttgcttttag ctagaccaat taaaaatcac aaagtgagcg tctctatcca ttggtagagg
      421 ggctacttgg catagtgtac attatgtcta ctaaccttta aaataacacg ttttataaaa
      481 taaaccctct tgacaatatt taattttctg attattctaa atatatacag aaagcgcttt
      541 cctaggattt tatatctaag gaggaagtaa caatgaaaaa taaaattatt aaaattatta
      601 cttgtctatt tttgttcggt atgattgttg gagcaagtac tccgttaaat ctgtttcctt
      661 ttagtgctag aggtataaaa gtggaagctg caagttcacg atataatcat aatcatcgtg
      721 gatttacttg ctataaaatg aactattatg ttactaataa aatgtgtaga gatttgaaaa
      781 aaaattataa taagctaaag actcctgctc aaattgcatc ttttattcct attggtggag
      841 ttggaacatg ggttattaca aatgctttta gtagtgctgt tgatgcaatg aatgtctttg
      901 taagagctgc aaatcaagga aaaggtgtac aattaacata taatgctcac tttagcaata
      961 caacatcata tcaatacaat gattacgctc gttatgtaat aaaataatca gaaagtgaaa
     1021 ctatctcatg aataaaaaga ttttagctgt aattatttca ataatagttt taatcactat
     1081 gttcttcata tttaggctaa ttttcaatat aagcttacaa aaaagtattc tttatctaat
     1141 tcctatagca tttatcttag caatttttag aagtatttac ggaaataaaa actctaaata
     1201 gctaaaaata aatcatacta aattttacaa aaagtggcta gcattttcta gccacttttt
     1261 gtattgtgga gcgtgggttt tgagggggat tcaaggggga gcttgcgccc ctttgacaag
     1321 cggttatttg caccaaatac ggaggtattt ggctagcaaa tatagagctt gccaaaccct
     1381 tttagtgagt tttgtagaaa gtacctttaa aggtactttc acattacgaa cgatacgtcc
     1441 gtaggacgta ctgaaagggt gggacttatg agcaaattaa tgcaaaattt ggctagcaaa
     1501 aaagctaaaa aatatatgct aataatcgag cgaaataaac actttttcaa gtgagcgaat
     1561 ccgccatgac aaagctgaag cagtttattt cagctttgtc atggcgtgtg ccgacttttt
     1621 taaaagtcta ggcacacgag caaatatctt tatcaagctc taatttgcgt tttaagacgt
     1681 ttgaaacgcc taagtatata aatatataag aataggttta agagtgctta aatcgaattt
     1741 tagagggcat ttagacaatt ttagggttgt tagggcttgc cctgaccgcc tgtaaggcgt
     1801 tttaagaaca agataaggga tttatactag gactgtctta aaatgtctta aacgaacgct
     1861 cagagcttct caaagggttc cttatgaacg gcttttagca aaggagccgg aggcgactgc
     1921 gcagtataat gtgagagcag agcgatcaca cctttttagc gaaccgtagg gagcaaggtt
     1981 tggaattggc agttttgata aattcgcgaa cctgtttaga gaaaaaagga gtgatttttt
     2041 aggcgggaaa attagcctcc cctcttttta tatctctttt tatatctctt tttatatctc
     2101 ttttatgtcg gctgtgtcct tactctccca agcaattcag agttttttca agtaggtttt
     2161 ctacgctttt caagtaggtt ttctacgctt ttcaagtagg ttttctacgc ttttcaagta
     2221 ggttttccga cattattgag tatttttata aagtatgtta cttttatttc atactttata
     2281 aaagtaggag aagcttatgg caaatgaaat tgtaaaatat gaaaatagat taaatcatat
     2341 tcctctgaga aaatttaata gtcgtgaaat gaacttattt ttttcaattg cttcacgtgt
     2401 acgtgaaaaa ggtgtagatg agattatttt ttcatttcac gatttgaaaa aactaagcgg
     2461 ttatacagaa cgtggggaac aatttataaa agatcttagt agcacgtatg acaaattatt
     2521 aagtattaat gcttggaatg atgatggaaa tactttaact aaatttgttg ctttcacaaa
     2581 atatagcatt atacgagata aacaacttgt tataatttca gttaatccag aatttaaagg
     2641 actttttaat caattaaaga actggacaag atttaatcta gagcaattta ctaatttgga
     2701 tagcacatat tcaaaaacta tgtttcgatt attaaaacaa tatcgaactc aaggctggac
     2761 tgaattttcc aaagaaaaat tttttgattt actagatatt cctaaaagtt attggaatag
     2821 tccgtcaaat attgacaaat ttatcttaag gccaattaaa gaagaactca ctcccttatt
     2881 taaaggatta actattcgta aaaaatatgg taaaggtcga ggtaaacctg ttatcggtta
     2941 tcgttttact tggaaagctg aaattaacca tgctgacgat ttttccaaag ggaaacaaga
     3001 agatttacgt ataaaattat ttaatattga tcacaatgga gaattaacac gagaagaaaa
     3061 atggcgcgct aaagatcgaa tactaaatct tcccttaggc actcatgaag ctgattttaa
     3121 acaacaacaa caaactgaag aaaaacaggc agaaaaacga gaactttcaa acaaactcaa
     3181 acaagattta ctaggaaatt tacaaaactt attcgattaa gcttaaggag tatttatggc
     3241 agataaaaca atcaaagaac ttgcggaaga attaggagtt agtaaaacag caattaacaa
     3301 aaaagtttct gacaaaaatc gaaaactttg gtttgcaaaa aacggaaaca agtttgtgat
     3361 taacgaaacc ggtcaaaaag ctataaaatc aatgttttta gtcgataacg caaacttaaa
     3421 tcgaaaacta gttggcgaaa aatcaaaaac caaaaaccat aaaaacacca accaagtttc
     3481 cgaattaaaa ctcctttctt tcctagaaaa acagttaaat cgaaaagata tacagctaca
     3541 agaaaaagat aatcaaataa aacaaatgca aaaattactc gaccagcaac aacaactcac
     3601 tctacaagcg aaccaacaaa ttgagaacct acaagaacag ctccatttaa cttatacaga
     3661 agaaactcca acaaccgaat caactgcctt ttctgaaaaa aaagcagaca atacacaacc
     3721 acttattgaa aaaaagtggt ggcaaatttg gaaatctaat agaaccaatc tctaaaattt
     3781 tctagaagtt agttcggata gtagaggaaa aagttgtact acctgatggg gagatcactc
     3841 aaatttttat gagaaagaaa aaatagttta caactgtaaa caatcaatgt ttatagttgt
     3901 aaactatcaa actatatggt gacttattgc ttaaaatagg ttgaaatctc actcaatttt
     3961 tggtggaaaa acaaaataaa ttctaatcga ttttgttgaa aaagtcataa aaaaacaaat
     4021 gtgattttaa caaaatttca aaaaaatcgt ttgaaatttc gtttattttt tttgagaatt
     4081 ttgatcatcg tagggtcatt gtagaatcat tatgagatca tcgtgggatc atcgaataaa
     4141 gtatgggaaa ggcttgcggt gcttggtatt aagggcattc tccgcctccc aggttcttga
     4201 ccattttctc tcaacgtggt aaaaatccca cttataagag aaaatggtca agaacctaaa
     4261 cggtcggcga agaaaacata ccaagcacca caagcaaaaa aagttagagt aacttccaaa
     4321 aaagaaaaat tttttagaac aacttttgaa aatttaattt tgatttatat ccgttcaatt
     4381 taaacatttg ataaactgaa ggctagtatt taaaatgtgt atacaagtgg tagcgttaaa
     4441 aaaagtggag gtataaccta tgtt
//
LOCUS       DAZUMK010000168         4378 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000168 DAZUMK010000000
VERSION     DAZUMK010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.173"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(42..938)
                     /locus_tag="ACG808_002706"
     CDS             complement(42..938)
                     /locus_tag="ACG808_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS5048288.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(931..1611)
                     /locus_tag="ACG808_002707"
     CDS             complement(931..1611)
                     /locus_tag="ACG808_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5048289.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(1612..2394)
                     /locus_tag="ACG808_002708"
     CDS             complement(1612..2394)
                     /locus_tag="ACG808_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS5048290.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(2387..3304)
                     /locus_tag="ACG808_002709"
     CDS             complement(2387..3304)
                     /locus_tag="ACG808_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS5048291.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(3468..3722)
                     /locus_tag="ACG808_002710"
     CDS             complement(3468..3722)
                     /locus_tag="ACG808_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048292.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
BASE COUNT         1285 a          951 c          762 g         1380 t
ORIGIN      
        1 gataatacat ttttctacta gttctcacta atcttgtata attaaaccct ccgcaacttg
       61 acggagatgg agaagatgcc cccgtctaat ctagcggtca tctctcctcc cattccttcc
      121 accaattttt ttgacaggta aagcccgatt ccgctagatt ccgtatttgt gcggctggta
      181 ttttcggtat aaaaacgctg gaagactttt tcaacagagg tttgcgttcc tggtttgata
      241 ggattggcca ctgttaatcg aatgaatgga tcctcttttg aaaatgtcac ctttgcttgt
      301 ccatctgcat aacgcaataa attctgtatg atattttgca gcactcgcag caacatgagc
      361 ggatctgcta atacagttgt atttatcgag tgttcagcga acgctacctc gatatttttc
      421 tcagaaaatt gttcataaaa agagaaaaat acttcttcca cctctttaga aaaagaaagc
      481 gtatggagat ccatttcttt ttgcttcgtc tctattcgag caagatcata aaactgatcc
      541 gtcatactga gtaatcttgc aacagattgt ttgatgatcg ttaaatactt ctcccgtttc
      601 tccgtatcct ctacctgttc cagtaattgt acataaccgt taatagcagt caaaggagtt
      661 ctgagatcat gagaaaggcc gacgatcgat agtctgatgt tttcttccat ttcttgaaat
      721 gcttgtcctt tttgttcatt ttcatccaac catgtattca gtgcatctac taggtccatt
      781 agattctttt ctcgaaattc gactgaaaga cgaccaccat attcttgtcg ttcagaaaga
      841 gtggcgattt tcttagttaa tctcttgatt tccctttgca ggaagaaaat aaaacttccc
      901 ataaagataa caacgagaag caacagccat ttaatcaaat ctaaacccca ctccccacac
      961 cgtatgaata tacacagcat gtgcctttgc tagcttccct cgaagattgg aaatatgcat
     1021 attcactgtt ttctcactat caaaatatgg ttcttgccaa actgtttcat aaagatttgc
     1081 tcgcgaaaat acttttttcg gatgttctaa aaaaagcttc agcagatcga attctcttcc
     1141 tgtcaaatgg acagccacac cattcacatt tacatccctc gactctttgt ccagtaagat
     1201 ttccttgtaa tagatgcgtt gcgacagatc ttttacttgg acagaagtct gatgacgaag
     1261 ttggatgccg attcgtaaca aaagttcttc gatatcaaat ggtttcgtca catagtcatc
     1321 cgctcctgct gccaacaagg aaactttatc tctttgatcg ctttttgctg aaagaacgat
     1381 gatcggaaca ttcgatgtct gtcggatttc tgctaacaat gcttcacctg aaagccctgg
     1441 aagcatgaga tctagaagga tcaaatcgac aggatatgtg cttaacaata attttccttc
     1501 cgtacctgaa taagcatcaa gaagtttgta tctctgttca agaacctcct ccagtaagga
     1561 atgaacagct tcatcgtctt cgataatcaa aatagtttcc agcaggacca cctacttaat
     1621 gtcagatttt tggaaaataa acattcctag agctgatgat accacgattg ttactagact
     1681 gaccagaatc attttcggtg cttgcgaata agttgcttgt tgcgcaaagt ccgtatacat
     1741 gagccacgtt tccgtaaacc actccgttag aaattccaag cctttgattt ttgcgaaaac
     1801 accgggaatg aaggccgttc ccatgatata aagaaaagaa agaagcaaac tgacactggt
     1861 acttctagtg atgaatgtca aagccactgg aacagataat gtccctaatg tcagcagaag
     1921 caatacagga aatactttta gaacataatt tccatagttt ttgattccct caacttcacc
     1981 aaatccgaaa gcaagtgtgt aagcaagcat ggctagtccc gtgatcaaaa ccacgccaag
     2041 tgttgtgatc aaaagcgtaa cgagaagttt cgacacataa taagtcattc gattccgtcc
     2101 taaagataac gtattgcgaa tcgtaccgtt ttgaaattct tccccaatat aaaagcacgc
     2161 aaatgctgcg aaagccagaa gaatccatgc agccattttt tctaccatta aaatccctgt
     2221 tgcaccgttg atgactgtat ccgttgcttg gtttaattca ttgccgtatt ttgtatgcat
     2281 ataccattcg ttccaagaat aaaaaagcgg aagtacgacc atcagtccta gtgagaccca
     2341 cattttacgg ctcttccata cttttatttt ttcagcagtt aatagattaa acatgtggat
     2401 tgcctccagt taattgtaag aaataatctt ccagtttttg cccaactagg tgaataccat
     2461 ccacaggaac ttttgcttct accaatgctt gattgatggc tgctacttgg tcaattcctt
     2521 caaaaagctg gataacacgg ctagattgta caaaataatc cctataacca agagagtcga
     2581 gaaccgttac tgcttgttcc gtcgccgaag tgtcaatttt tataaattga cgagattctt
     2641 gtgccaattc ttctttcgat aattctttga tcaagtgtcc ttcatgcaaa ataccatagt
     2701 gagtggcgat ctgtgacaat tcatctagca agtggctaga aagcaagacg gtgatgcccc
     2761 gttcttttac caaacgttgg atgatttctc gcatttctgc catccctgct ggatccatcc
     2821 cattcgtcgg ttcatccaaa atcaaaaatt ctggattcgt gatcagtgca tttgcaatag
     2881 ctaaacgctg gcgcatacct aaagagaaat tctttgcttt ctttttccct gtattttcca
     2941 atcgcatcat tttcagcagg tcttcgattt ctctatccga cacaccgccg ttcgctgctt
     3001 ggatacgtag gttatctctt gcagttaatt ctggataaag agcaggggtt tcaatcgatt
     3061 gacccatacg tcgtcttgct gcctgcaaat ccgtcgttcc aaataattcg atcgaacctt
     3121 catcaatcga gatcaaaccc ataatagttc tcataaaggt agacttaccc gctccatttt
     3181 ccccaatcaa tccatagatc attccacgtt tgatttcgat tgatacttta tctaatgctt
     3241 tggcagcacc gtattttttg gttataccag ttgctttcaa gaccgtttca tttccaaaat
     3301 ccatacttat ccttctttct tattgatgag tgaagtatag ttgacgggta taaagaaagc
     3361 agccagaaat gtataaagaa agtgtaaatt ttccaattga atgtcaaaaa aaacaatcgg
     3421 acaatacgtg tctttcctat tatcaggcag tttgttatac gcttgtatca gtttcctgat
     3481 tctttctttt cgaccaaagt cgtttctctg tattcctctt cactgaaaat ttttgcttct
     3541 ttcagatcaa gcttgagtaa ttttaccaga ccggttataa ggataactat agcaagactc
     3601 cctcccagaa aaattagact gtctcgcaga cgtggttgcg ttgtcttcca atcaaagagc
     3661 gcaccaaaca gtgctaatcc aagtggactg gccaccatgg taatcgttgt tatcgttgac
     3721 ataagtcgtc cttgtatatg cgttggaact tctcgctgaa gccagaccat ataaaaagca
     3781 aaagaaaaca ttaatcctta aaaacggact tatgaaaaat aatacatgcc gtttgggatt
     3841 tacaattagc taccaaataa gaggttatga caaaagactt ttcacaatcc cttcttccga
     3901 ataaacggtg ttctcctgcc aactcctcgg tcacaagtca caatatttat caaacatgag
     3961 aagcaatttt tgtaaattcc ttcttatttc ttgaagctga ccacttctgt cacaacctca
     4021 gaagcggata aataatattg tttgatgata ctttatcttt ttcccgtttg tttatagatc
     4081 gaatgatatt atagaccaag aatcctacct attaacatag ctaaaaacac aaaaaaggca
     4141 ctttccaaat tgtggaaagt gccagaaaag ttaggattaa ggtaactgcg ctcagcattg
     4201 cttcgcccag tacgattctc gtaactcgtg aactcgaacg ataatcgcaa ttaacgtttt
     4261 gagaactgtg aagctttacg ggctttctta agacctggtt ttttcttcgt atcattgaat
     4321 aagtttgaaa aaccttgatt tataaaggtt ttcccgtaaa tatattttat tatatttg
//
LOCUS       DAZUMK010000169         4345 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000169 DAZUMK010000000
VERSION     DAZUMK010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.174"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            17..898
                     /locus_tag="ACG808_002711"
     CDS             17..898
                     /locus_tag="ACG808_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS5048293.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            911..2521
                     /locus_tag="ACG808_002712"
     CDS             911..2521
                     /locus_tag="ACG808_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5048294.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2598..3257)
                     /locus_tag="ACG808_002713"
     CDS             complement(2598..3257)
                     /locus_tag="ACG808_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS5048295.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3540..4205)
                     /locus_tag="ACG808_002714"
     CDS             complement(3540..4205)
                     /locus_tag="ACG808_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFS5048296.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
BASE COUNT         1159 a          855 c          865 g         1466 t
ORIGIN      
        1 aaggagtggg gtttttatgg cagaaatcgt caaagtcaca catttgaaaa agagctttgg
       61 tcgtttcgaa gctttaaaag atgtctcttt caccgtacat tcaggtgaag tcgttggatt
      121 tatcggacca aatggttctg gaaaatccac gacgattcgg acacttttag ggattataaa
      181 aaaagacggc ggaacggcaa cgatttttgg gcaagatgta tgggaagata gtttatctat
      241 ccatgaacgt ctttcttatg ttccaggcga tatctctttg tggggaaatt tgactggagg
      301 agcgatcatc gatctcttca tgaagcttca tggaaaaggg agtgctcaaa aaaaagatga
      361 actgattcaa cgtttctctc ttgatcctaa gaaaaaagca aaaaactatt caaaaggaaa
      421 tcgccaaaaa gtgggcttga ttgctgcttt ggctgttgat tcggatctct atttatttga
      481 tgagcctact tctggtttag atcctttgat ggaaacgatt ttccaagaag aagttgaaaa
      541 attgaaagaa cagggcaaat ccatcctact ttcttctcat attctcagtg aagtcgagcg
      601 actagcggat aaagtcgtga tcatacgcga aggaacactc gatgaagaag gaacactcga
      661 tgaattgcgt catttgactc gttcgaccat cactatagaa acagaagcag agaatagtca
      721 cttagcacaa ttgaacggtg tccatgactt tgtgcaaaaa ggtcacctcg ctcaatttgc
      781 tgctgataac gaagctttcg atgtgatttt agctgaagcg tccaaattag gtgtgaaaaa
      841 atttgaatct attccaccga ctcttgagga tttattcatg agacattatg aaggatgagg
      901 aggaagcgaa atgactgata aattttcacg ctggtccacc cttttttctc aaaaccttag
      961 ccgtgactgg aaaaagatcc tgatatggct agtcggtgtc actgcttttt ctggtggttt
     1021 tgtccctgcg tttgaagaat tgacttccgg acaaggatta gctggattat acgaaacctt
     1081 gcaaaacccg gcaatgattg caatggtcgg accaactcca gcttcgaatg ctgctgagta
     1141 tactttagga gcgatgtatg caaatgaaat gttgcttttt tgcagtttgt ttgctgctgt
     1201 gatctctgtt ttacacgtgg tgagccaaac gagaaaaaaa gaagaacttg gtctgattga
     1261 acttattcgt tcctttcgag tcggacgtca agcaaactct ttagctgtca tcgctgaaag
     1321 tattctgatc aatttgcttt tagcaatatc gatcagctgt acgatgattt ttttccaagc
     1381 ggattcgatc actgcagaag gtgcttggct ttttgggcta tctgtaggta gtatggggat
     1441 tttggggagt gccgttgcac tgtttgctgc acaactgatg ccaagttcag ccagttcgaa
     1501 tggtctgtct ttagcgtttt taggtggact ttaccttcta cgagcaataa cagatgtagc
     1561 tgattcctcc ctttcgatgt ggaatccttt aggctggtct tatttgacct atccatttac
     1621 aactaacaac tggattcctt tgctttattt gtttttgttc agtctaatct tgataatcag
     1681 ttcatttgtt ttagaggaac atcgagattt gaacaccggc tatatccccg aacgtgaagg
     1741 aaaaccgtat gctaaacgtt ctcttttatc cttgcgtggt ttattattct atctaaataa
     1801 agggatgaca atcagttggc ttatcacttt tggtttgatg ggggcggcat acggctctat
     1861 ctacgggaat atgcaacagt ttttagaaag taacgactta atgaaacaga tgtttatcca
     1921 atctggcgtc tcaattgaaa aaaactttac aagcacgatc atggtcgtct tgatttgtct
     1981 agctgctatt ttaccaattg ctagcatcaa tcgtttattt acagaagaaa cccacggacg
     2041 tatgaatcag ctgttcgcaa caaaaatttc taggaaccaa ctttattgga ccaatatgtt
     2101 actagctatt ctatttgcta tattggcaat cctgattagt tctggtgcac tagggctttc
     2161 tgctttggct tcgatgaagg atgcgaaaga catggatcta ttgatgtttc ttaaagccgg
     2221 gttcaatttc ctgcctgccg ttttagtgtt tatcggaatt agtggctttc tattcggatg
     2281 gcttcctcga atcggtaaac ttgcttatat ctacttagct tactcctttg cactcaatta
     2341 ctttggcgga attttggatt taccagaatg gttttccaaa acagctgtcc ctagctgggt
     2401 tccacggatg ccaaacgatt cttttgatct ttccgtgttc cttacgttat catgtatcag
     2461 ccttgcactg ttcgtccttg gttctattgg ttataggcga agagacttac tagaaggata
     2521 ataaaaaagc atgtacaatt tcatgctagc ctccaaatct gggtcaaaaa tccaacattt
     2581 ggaggtcatt tttttattta caagtcttct aactgctctt tatcaatgat ccagacttct
     2641 cgattctttc tttttacata accattctcc tctaataatt taaattttct agataaagtt
     2701 tcgggggtcg taccgataaa tgcagcaagt tctttcattt tcattggcag gaaaaagtga
     2761 tccttccctg tcgctttagc taagtcaagc agatagacag cgactcgttc ttctactttt
     2821 tccatcatca aaaaagttgt ctgctgctgt acttttgtta atttctcaac gttgattgct
     2881 aaaagtttca gactaagggc gggataatgc agtaagagtt ccgagaaatc tttttgtctc
     2941 aatatgcaaa tttccgtttt ttctagtgct tcgccaaata aagactcatt tggaacgcct
     3001 agcaactggc tctctccctc atatccgccc ggctctatta ctcgtaaaag ctgttccttt
     3061 ccgctagcag ataattgata cactttcata ctgccggagg ccacgatcac taattgaggt
     3121 tctccattcg gcgcaagaat ctgttctcct ttttccacat agtgatgttt gacaagtgcg
     3181 tgtacttttt cttgatcttg ctgtactaag tgattgaaca aaggaacaaa tgaaacacat
     3241 aaatgatcat gactcataat ctctcctcct tctaaacagc tatttctaat aaagaaacat
     3301 aacttccctt attccaactg ataattttct tttatcatag cataaacgtc aagcgaacca
     3361 gataccagca aatagagtgg atttcttttt gtcccttgct ttattttcct tcttttgaga
     3421 ccttaagtaa ttgattccta tcaatttttt aaatatttac tttttgaata atcaaaaaca
     3481 aaccttcact cgcaagccga gagtaaggtt tgtttttcgc attgcttatt tcgtgttgtc
     3541 tattgaaatt tttccgcacc tagcttttga tatagtggtg ctagttcttg taaggtttca
     3601 gcggttattt gctcaacgtg ttcgatttct tgcggataga agaatagttt acccatttga
     3661 aaggttcggt ctctcatcgc ttttttcaac tcttcttgtt tctttgtatc ggacaaatag
     3721 tcagacaaag ctaagtggtc gtcaagttca tgttctggtt gcggccatat gtaataatcc
     3781 ttgatatcga ctgttttgct ccactctttc agtatcgcaa gcaactggtt atacgctgca
     3841 atgctaccat atcgttcttc gcttttataa tgttggtaca tcaaatagat ttggtattgt
     3901 ttttgattca acaaaactgc tagacaggca ttctcgtctt gacgagattc gcttcggtac
     3961 gcgctccaaa aatgactgcg taaattccag ccattcgtcc agctttcgat tttaggtttg
     4021 ctcatgccgt acatatcagg caaaagtgca gctgtctgga gctgaatctc tttccatttt
     4081 tgccagtgtt ctttatattc tttcttgatt tgttcaatct tatctgttgg atggctctct
     4141 ttgagctgtt tgaatgcgta gattggtcga tcaaacaact gataatctga ttttccaagt
     4201 tccattgttt cgcctctttc cgttatcttt tgtattctac ctgaattttg cgatatggac
     4261 aaatagaggt tgtgaaaaag ctgttctgca tcaagtcata agaacagcca aacaaaaaac
     4321 tgcgatgagc atcaccgcag tttta
//
LOCUS       DAZUMK010000170         4232 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000170 DAZUMK010000000
VERSION     DAZUMK010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.175"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            49..1350
                     /locus_tag="ACG808_002715"
     CDS             49..1350
                     /locus_tag="ACG808_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5048297.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1377..1637
                     /locus_tag="ACG808_002716"
     CDS             1377..1637
                     /locus_tag="ACG808_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFS5048298.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVTHLNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2636..3871)
                     /locus_tag="ACG808_002717"
     CDS             complement(2636..3871)
                     /locus_tag="ACG808_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS5048299.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1225 a          678 c          877 g         1452 t
ORIGIN      
        1 ttgtcatgca atgaatggtt catattttat tgaaaaggag tggttttagt ggaaaagctg
       61 atgaaatggg tcgaagagaa acttgtccca ccaatggcaa aaattggtac acagcgtcat
      121 ttattagcaa ttcgtaatgg ggttgtttca acattgtcat tgattttgat tgggacattt
      181 tttatggtct ttatcaattt accgtttcct ggatggaatg aatttattgc cccttattcg
      241 gcaacgattg ttttaccctt taggatgacg atgggattga tggctattta tgcaactttt
      301 gttatgggtt cggatctagc taaatcatat ggattagata gtgtgacagg tggtatatta
      361 tctttaggaa cgttctttat gttacaagtt cctgtaaacg ttttaactcc tgaagaggca
      421 cctcttggct gggtcttacc tatgagttct ttgggagcat ctgggatgtt tgcggggatc
      481 ttatctatga tttttgcagt ggaagtatat cgtttcttta aaaatcgaaa tattactatc
      541 aaaatgccag aacaagtgcc acctgctgtt gcccgtagtt ttgaggcatt gattccaggt
      601 gcagtcattt taacaattac ttggctgatt cgaagcgtcg cgggctttga tgtaaacgct
      661 gccttattat cgttattcga gcctttaacc aatattttag ggaataattt gcttggtgtt
      721 ttactaccaa tgtttttgat ccatttgttg tggtcatttg gtatccatgg catgagtatt
      781 gtaggggctg tcgttcgtcc aatgtggctg atcatgttgg atgagaatgc aaaggcattg
      841 gcagatggta cagctgccac ccaattaccc tatatcgctc ctgaacagtt ttatcaatgg
      901 acggtaacaa ttggtggtgc aggagcaacg atcgtggtat ctgttttgtt tatattcttc
      961 tgtaaatcaa aattcttgaa agaggttggt cgtttttcta ttattcctgg tatcttcaat
     1021 atcaatgagc ccataatttt tggagctcct atgatgttga atccttacat gttcatcccg
     1081 ttcaacttag ttcctttagt tttgactatt atcagttatt ttgcagtcaa attagaaatg
     1141 gtcaatagat ttactgtttt gccaacatgg acattaccag ctccaatcgg cggttatctt
     1201 tcagcaggaa atgactggcg tgttgttatc ttgatcatga tcaacacatt gatagcattt
     1261 attatctatt atccatttgt aaaagcttat gataaaaaga tgctgtcaga tgaacaaagt
     1321 cgtggagaaa acgaagtcca gactgtctaa gtttggtttt tagaaggtgg gaaacaatgt
     1381 attggctgat tttatttttt gtttttatct ttttattaac ggctagtcac cttatattga
     1441 atatgctagc tacatatcat attcaaatca atcgatggat ttgggcactg gcatcatttt
     1501 tgatcgtgat tcttccaaag atcattgtaa cgcatttgaa tgttttgttt tcatggggga
     1561 catatgtact ttgtggaatt tttgctatca attttatgat cgagcagcat agatggtttg
     1621 tcacatcgaa gctttaaatg ttctaaaagt ggttgtaaca gaagtggtta gcttcaagaa
     1681 ataagaagaa atttacgaaa attgcttctt aaatttttgt gaaatgttgg cttatttttc
     1741 gtttggttga acttattacc gcagatgcaa gcttttgacc accgtttatt cggaatatgg
     1801 gactgtgaca aggcttttgt cacagccctt ttttacataa agattattat tgttttaact
     1861 gttttggcag aaagaaggag gaaaaaatgg aaattttatc agcaatggat ttagaattag
     1921 tagatctact accaaagtga tcaagtcaaa tatagatttg ggagcttaca accatatagt
     1981 aggggctgca gtatggtgta tgaatggtaa tatgtatgat gtgagtgttt attcaaatca
     2041 tgaaacgtgt atagaaataa ttgtgctggg aatagcaatc agtcatggtg aacgtgactt
     2101 ttagtgtatc gttgctgacg acatagaaca ttctgacatg atctactcaa tatgtgggga
     2161 ttgctggcag tttatatagg agtatgcatc ggattgcgag atcattgtct caacagaaga
     2221 gggaggttga ttatcaaaga taaagtggta aatcctacat ttgatttagt aattgttgag
     2281 gggactagcg gcacaaatct ctacaaagcg atgcttctag atctatatga cacattttaa
     2341 gaaaatgtat ttgcctaatt attatggtct ttcttttgaa gaaacgttga gatgatccaa
     2401 ccagagaaag gtgtcaaaga atttgactaa gaataaatgg cagacgctac ctatgctcat
     2461 ttggatagcg tcagatcttt tattttaagg atgattttgt gttttttaga caccgtaatt
     2521 taagtaactc taacttttca atccactatt tacttttttt gtattttcac tgtctgggtc
     2581 tcgaatagcg aaattactga tgagtctcta ttaaaaaagc tttttctact tgaaactata
     2641 ttgggtggtt gctcaggaag gtttcgattt cttgaattgc ttgttgtgga ccatttattg
     2701 gaaaaacggc ttgcagcgtg cgtgcctgct gacgaatctt gggtgaagaa acttcgcgca
     2761 gagcaatctg cagttttctt tcagtcactg gttttgtgag ggcgattgcg tttccgtatt
     2821 ttacacaata gtcaatatta cagctttgct caaattgcat ccccacaccg ataaacggtt
     2881 tgccggattc acaggcagtt tggacggtcc cttccccgcc gtggataaaa gaaaaatcaa
     2941 tgaattcatg caaaagatga ctagacagat attctgttat taaaagatct tctgagtcag
     3001 attgaatgtc atcaagcaga tagtcaatag gtgcaataac cgctagatcc ggttgttttt
     3061 gtaagaagaa caaaattttg cgtaacatac gcggattgcc tgaactaccc attgcgaaat
     3121 agataatacg ttttctttct tgttgctttc gctgtataaa agttagcact tcttcaggca
     3181 aggaagtact gagcttagca aaaattggac caacgattcg ataattttcc ggcagctgtt
     3241 cttcttccaa aaatagttta ggcgttgtga tgagatttag atcagcatcc atcatttgta
     3301 aacttgtttt tggtaaagga agcatttctt tttgtgcagc gtgtttgaag gcattgggga
     3361 tccatcgggt atttactata cgcttttttg tccagtgcca aaggggattg cttaaagggc
     3421 cgaagaaacg taactcgtgg ggcatcagcg catgctctga ttgaaaaaaa ggttcactca
     3481 aagcataggg tttaacatat actaaaggaa tctttttatt gcgggccgat aaaaaaattg
     3541 tcacattcga acctgtcaca atcagtcgag gctcaaactt agcaatcaga ctctgttcac
     3601 tagtgatacg agaggcaacc atttctttgg taaaagggtt tttaagtgta cgcaactggt
     3661 caaattttat gatttgttct tcttgttttt tcgtaaggat cggcgctagt aaatgatact
     3721 caaagccact ttctgtgatc aaaggagcaa aagttttaga aaagccaaag aatacacagt
     3781 aataacggtc tttcatagct ttggcaacct caatcattcg tgttgtctca gctaaattga
     3841 atactgcagg agcaaaaaga attgtcttca tgaaatcctc cttattggaa tgagtctatc
     3901 ccttcgtttc ttactagtat actaaaaaac acagcgttcg agtagcctat gtatattttg
     3961 aatattaaac tgtctctcgc tgttttcaca acttctaaat ttaaattttg taatcaagtt
     4021 gaaactccaa ttttcaaaaa aacacgcaac aattttttga tcgatttata tgttgtgcca
     4081 gataaatacg agaagttgtg tcattaaaaa taaacatgag aacataaaaa aataaaataa
     4141 ttatattaat tgataggtgt gctgtcgtca ttatacacac gtaagaaaga aatagttatg
     4201 tttaaaaata ttcttagact ttctcgccat ga
//
LOCUS       DAZUMK010000171         4096 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000171 DAZUMK010000000
VERSION     DAZUMK010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.176"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(78..203)
                     /locus_tag="ACG808_002718"
     CDS             complement(78..203)
                     /locus_tag="ACG808_002718"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048300.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLTELGRSRPSSIGG"
     gene            complement(449..2446)
                     /gene="tkt"
                     /locus_tag="ACG808_002719"
     CDS             complement(449..2446)
                     /gene="tkt"
                     /locus_tag="ACG808_002719"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFS5048301.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPIGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2548..2799)
                     /locus_tag="ACG808_002720"
     CDS             complement(2548..2799)
                     /locus_tag="ACG808_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFS5048302.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2946..3569
                     /gene="lexA"
                     /locus_tag="ACG808_002721"
     CDS             2946..3569
                     /gene="lexA"
                     /locus_tag="ACG808_002721"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFS5048303.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3748..3966
                     /locus_tag="ACG808_002722"
     CDS             3748..3966
                     /locus_tag="ACG808_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048304.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1142 a          875 c          750 g         1329 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaaggagcaa tcgctttttt attgatcacc atttttcttt taggtctatg gtgtcgggca
      181 ctgcgggtct tcagctcttg cattcaaatt cctcctcaaa ataaaaaaac ttaataaaaa
      241 gttctcttct taaaacaaac ttaattaatt aaattatact tttaattctc aagaaacaca
      301 aactaatccg aagaattctt tgttttgtca tttaaatgta acatagccga aaacgaaaag
      361 taatattgat ttattcttct aacacataaa gaacagctgt ctccaacttg cagacagctg
      421 ttcttatcag tcaaacaatt gcttggatct atgatagttg attgtacgta ttcacgacat
      481 tttctactgt aaatccaaat tcttctaaaa ttttagtgcc aggggcagat gcaccgaaat
      541 gatcgattgt aatcgttgcg ccctctgttc ctacgtagcg ttcccaacca aatgatgcag
      601 cagcttcgat agccacacgt ttcttcactg atttaggaag tacactttct ttatattcag
      661 aagattgttt ttcaaagagg tcaaagcttg gcatagaaac aacagaaaca tctttgcctt
      721 tttcagccaa ttctttttga gctttcacag ccaaatcaac ttcagaacct gttgcaatca
      781 aaataccttc tggtgtttct ccttgtgatg gagaaagaac atacgcgcct tttttcacca
      841 tttcatctgc tacttctttt gtagaaggca atactggaag gttttgacga ctcaatacaa
      901 ggatagtagg cgcatctgtt gtctccatcg ctactttcca agcagcacgt gtttcatttc
      961 cgtctgccgg acgaatcact tgaacaccag gcatgcagcg aacagatgct agttgttcaa
     1021 tcggttcgtg cgttggtccg tcttctccga cagcaaccga gtcatgtgtt aagacgtaaa
     1081 caacaggagt atgttggatt gctgctaaac gaactgctgg tcttagataa tcgacaaaga
     1141 caaagaatgt tccaccgtag atacgagttc cgccatgaag ttggatacca ttcatagccg
     1201 aagccattgc aaattcgcgg acaccgaacc agatgttgcg tccttcataa tgttcaggag
     1261 tgaaatcttt atctgcagcc accattgtat tattggaacc tgacaagtcc gctgaaccac
     1321 cccagaagct tggtatagct ttcgataatt cttggatgac ttctttactt gaaacacggc
     1381 ttgcttgact gcttccaact tcataagttg gcaattctgc atcccagttt tctggtagtt
     1441 tgccatcaaa agcatcttca aattgttgtg ctaattcagg ataagctttt ttataattgg
     1501 caaacatttc attccatgca tcttcagctt tttgtccttc ttctatcatc gtttgatgga
     1561 agcgggcagc tacttcttct ggaacagtga aatctggata ctcccaacca taaacttctt
     1621 tagccatctt gatgccatct tcaccaatag gagcaccatg aacagcagat gttccttctt
     1681 taggagcacc atagccgatg actgttttta cttcgatcaa tgttggtttg tctgtttcag
     1741 cttttgcttc ttcaatcgct ttagagattg cttctaggtc atttccgtct ttcactaaga
     1801 tatgttgcca gccatatgct tcatagcgag caccaacgtt ttctgtgaaa gcttttgaag
     1861 ttggaccgtc tagtgaaata tcgtttgaat cgtataaaac gatcaatttc cctagtttca
     1921 tatgacctgc catcgaactt gcttcttgag ataccccttc catcaagtct ccgtcaccgc
     1981 atattgcata cgtatagtga tccatcacat taaagttttc tttgttatac gttgctgcaa
     2041 ggtgcgcttc tgccattgcc ataccgacag ccattgcaat cccttgtcct aatggccctg
     2101 tagtggcttc tacaccatct gtgtgatgta cttcaggatg tcctggtgtt ttagaatccc
     2161 attgacggaa ttgtttcaaa tcatcgattg tcacttgata gccagctaaa tgaagcaagc
     2221 tatacaacat tgctgaccca tgtcctgccg aaagaacgaa acggtcgcgg tcagcccagt
     2281 tttttgatgt cgttggattt actttcaggt gttttgtcca taacgcatat gccataggtg
     2341 ctgcgcccat cggcaatcca ggatgtcctg agtttgcttt ttggactgct tcaatactta
     2401 atgtacgaat tgtgttaacg ccaagttggt caattttgtc aaacaaaata acccctccta
     2461 ttaatttagg ttttttttac ctttatttct tacactttca ttctataaga tgtcacacga
     2521 aaaagcaatc tttttcagta ttttttacta aaagttgtca tttcggttgt gtaagccttt
     2581 tttcttttgg atttctttta atttttccgg ggtcacgtca ttgccctcag gatcgacgac
     2641 tttcattcct tcgatatgat gacgcatacc ggaacgaaaa gcttccaaat actctttgcg
     2701 taagttgatt tgttcttgtt cttcggcttt tgagagacca ttttccttct ttttacgtgc
     2761 aagttcattg atcctctcta ttttttcagg tgacagcatg ctcagccctc cttttactct
     2821 ccttcatatt atataaaaac tgaaaaaaaa acaactagat aaactaaata aaatacgaac
     2881 attcgtttga tttagcgctt tttatatggt actatttatt tatcaagtga aagaaggtgt
     2941 accttgtggc ccgacaaact gaatcaagac aaatcgaagt cttacgatat atccatgaac
     3001 aagtaaccca aaaaggatac ccgccgactg tccgtgaaat cggggaagct gttcagcttt
     3061 cttctacttc tactgttcac ggtcatcttg cacggcttga aaaaaaaggg tttatccaaa
     3121 gagatccgac gaaacctcgt gcaatcgagt tgacaaaaca aggtttagaa aagattggga
     3181 ttcgtccaac aactattcca gtacttggcg tcgtaacagc tggagaaccc attttagcag
     3241 tagaggatgc ttcagacttc tttccaatcc ctcctaatct ttcttccgaa gaaggcagtc
     3301 tctttatgct gacaattcgt ggagatagta tgatcaatgc tgggatcttg gatggtgaca
     3361 acgtgatcgt tcggaagcaa gaaactgctc aaaatggcga tattgtcatt gcaatgactg
     3421 ccgaggacga agcgacttgc aaacgattct ataaagaaaa aaactttttc cgtctgcagc
     3481 ctgaaaacga cttgctcgaa ccaatcatct tagatcaagt atctatatta ggacgagtag
     3541 taggacttta tcgtagtcat atctattaat tgctactaat aaaagaaaac tttttaaaaa
     3601 gagtttgaga cgatggtctc aaactctttt ttcgcccggg ttcttcttct ttcaaatttt
     3661 tatcacttcc ctctttacaa accgaacaaa tattcgtata ctaaatatac gaacaattgt
     3721 tcgcataaat taacgaggtg agaattcatg aaatcaatga gacgtcttgg cttagtatta
     3781 actgttttgt ttttaggtat tattattggt aatttcggca tacgtccagc tttacttgtg
     3841 tttgttccgt tatttacttt gtggttcatg ttgtgggatg aaaagaagta ccgtcaatct
     3901 gagcggcgca gaattcatga agatcatttc tatcatgagc cctactacag atccaggcaa
     3961 tcataataag gattgactgc taattattca aaagacaaga agttaaactt ttacgggggt
     4021 gtgacaaaaa tcggtcacat ccttttttcg aataaacggt gttctaaagc cagtttttcg
     4081 gtattaagtc aaattg
//
LOCUS       DAZUMK010000172         4066 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000172 DAZUMK010000000
VERSION     DAZUMK010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.177"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            119..1468
                     /locus_tag="ACG808_002723"
     CDS             119..1468
                     /locus_tag="ACG808_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS5048305.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1596..2186)
                     /locus_tag="ACG808_002724"
     CDS             complement(1596..2186)
                     /locus_tag="ACG808_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFS5048306.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2333..3685
                     /gene="brnQ"
                     /locus_tag="ACG808_002725"
     CDS             2333..3685
                     /gene="brnQ"
                     /locus_tag="ACG808_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFS5048307.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1168 a          745 c          789 g         1364 t
ORIGIN      
        1 ttttatgaat tactcataaa aggtaaattt ctgaaaattt cggaatctta gttgtgtttt
       61 tcagaaaact ctcttaatat ggaagtaatt aaactttttt agttaggatg atttaaaaat
      121 gaatgaagag caagaactgc agcgtggact gaaaaataga catgtccaac tgatttctat
      181 tggcggagcg attggaacgg ggttgttttt aggttccgga aagtctatcc agctagctgg
      241 cccttcgata ttgctcgcct atcttattac tggatgcatc tgttttttca ttatgcgtgc
      301 gttaggcgaa cttctgttat caaacaccaa taaccattct tttctggatt ttgttgcaga
      361 atatcttggc aaaaaagcgg ctttcatcac tggctggacg tattggttct gctgggtatc
      421 gattgcaatg gccgatttaa cagccatcgg aatgtatgta cgctactggg cgcctggtgt
      481 tcctcagtgg cttcctgaac tgattgcatt aggtcttctt ctttgcttga atatggtggc
      541 tgtctcattg tttggtgagt tagagttttg gtttgcattg atcaaagttg tagcgatcat
      601 tgcctttatc ctcgtaggag cttatatgat cctcacccac tatacgacag atattggcac
      661 agccagtatc acgaatctat ggtctcatgg cggattcttt ccaactggtg caaaaggatt
      721 tatcttagct ttccaaatgg taacctttgc ttttgctggg atagaattag ttggattagt
      781 cgctggagaa acagaaaatc ctgaaaaagt gttgccggaa gctatcaata atattcctat
      841 caggatcatc ttattttact taggatctct ttttgtcatt atggcaatct atccatggaa
      901 cagcttgaat ccagataaca gtccatttgt agaagtattt tcggaaattg ggatcacgat
      961 tgctgcttcc ttgatcaatt tagtcgtatt aagtgcagca gcttctgcct gcaacagtgc
     1021 aatatatagt acaggacgta tgcttcgttc tttagcgcaa gaaggcagtg cccctaaaaa
     1081 attcaaaaag ttaacaacac atcatgtgcc agggaatgct ttgacatttt ctactatcgt
     1141 cattttcatt tctgttatct tgaattacgt gatgcctagt gaagtattca cactagtaag
     1201 cagtattgcc actacttgtt tcttgtttat ctggagtatg cttgtctaca cgcacatgaa
     1261 atatcgtaaa agtatcctcg gcaaaaaagc tcattcattt aaaatgccgt tgtacccatt
     1321 tagtaattat ctggtatttc tttacatggc ctttgtgtgt gtcgtcttgt tcctaggaaa
     1381 agatacgaga atcgctttat tactaacacc tgtgtggttc ttgcttctat tactaatcta
     1441 tcatatgaaa tatgaacgta agaagtaaga aagtagttgt gacagaagtg gtcagcttcg
     1501 agaaataaga aggaatttac gaaaattgct tcttaaattt ttgtgaaatt tcggcttatt
     1561 tcttggagat aaaagcttct gtcccagcct cttttctatt tccccggaaa caaccgctga
     1621 taataggcat ctgctttttt ataggaaaaa atccaaatac cagatacaaa aaaacgtccg
     1681 aataacccga tcaacggtac aaaaaagaca ccggcaatca agcataagtt aatcatcatg
     1741 gagataagac ttcgacggtc ctttgacaaa actctcacga catgggaatc tggtttattt
     1801 gctttcatga tttgcctcat caagatacca aaagaaaaac tccacatcgt gtatactaag
     1861 acataagcca tctcaggcca aaaagcttca gggttacgcc cgacccaact agtaacagaa
     1921 ggacataaag tcagccaaaa gagcaaaaag atattcgacc agaccactcg gtaattaatt
     1981 cgttctgcca tctgaaatat acgatgatgg ttcacccaga tcaccgcaac aaatataaag
     2041 ctgatggtat aagctaataa tgtatttcgg atgacccata aagaatgaaa gctagatgta
     2101 ttagggatct tgatctctaa tacaataact ctcaaaacaa tggctaccac tccatcagta
     2161 aatgcttcta atctcgactt tgtcacgatg attcctcctc agatgattca gcgcttttat
     2221 tttatcactt acgttattaa ataaccatcg ttttatatag ggaattttct gaaaattgtt
     2281 gcatattttt cgaaattttg ttacttttaa aaaaaccgta aaggagttat gtatgacaaa
     2341 aaaattatca tttaaagact atctttttat aggctccatg cttttcggtc tattttttgg
     2401 tgctggaaat cttatctttc ccgttcattt gggtcaagaa gcaggcgctg ctaccttttg
     2461 ggcgaatcta ggtttcttgg ttaccggtat cggtcttccc ttcttagggg tgattgccat
     2521 tggtgtatcg aaaagctcag gtgtatatga attagcaaaa cggataaatc aaacatatgc
     2581 gatggttttt accatcttgc tttacttagt tatcggaccg ttttttgctt tacctcgcct
     2641 tgcaaccaca tctttcgaga ttggtttagc accatttatc gatcctagca gacagacgct
     2701 atatctagca attttcagta ttgtattttt tgtgctggct tggtggtttt caagaaaacc
     2761 gacaaagata ttagattatg ttggaaagtt cctgaatcct gcatttttgg ttttattagg
     2821 tgctttgctt ctccttgctt ttttgaatcc actaggttct gtaaatcaag cgcctattca
     2881 gccaaattat caagagcatg cttttttcac agggtttact caaggatata acacattaga
     2941 tgcgcttgct gctttagcat ttgggatcat cattgtcaca acaatccaaa acatgggtgt
     3001 aacaaaacct gctgaaatcg ctaaagacac gatcaaatct ggtgcgatca gcattatatt
     3061 gatgggaatc atttatactt tgctcgctta tttaggtgct atgagcttag gtagctttgc
     3121 tctaagtgaa aatggcggga tcacgctggc tcagattgct gatcattatt taggtactta
     3181 tggcagtatc ttattagcat ttatcgttat attagcttgc ttgaagaccg gtattggttt
     3241 gattacagct tttgcagaaa cattcacaga tttgtttccg caaagaaatt acgcattctt
     3301 tgttgcactt gctagtatcc ttccttgttt agcggcaaac gtaggcttga cgaatatcat
     3361 tcaattttcg ttgcctgttt tgatgttcat ctatccatta gcaatgacac tgattttact
     3421 agtgttagtt ggtcctttgt ttaagcagcg tacggctgtt tatcgaatga ctacttattt
     3481 tacattgatt gcttcgattt ttgatgggtt gaacgcttgc ccagaaacta tcaaacaaac
     3541 acctattgtt caaaatcttt tatatgttgc ggaaagctac ttgccgtttt tcaaattggg
     3601 gatggggtgg atcgtaccag cagttatcgg ttttgttatc ggactgattt ggagtttcgc
     3661 taagaaagaa gaagtagcag attgataaaa gaggttgtga aagagacgtt cagccttgag
     3721 caataaaaag taattttgaa aaatggcttc tcacattttt gcataaattt gacttaatac
     3781 cgagaaactg acttttgaac accgtttatt gagaggtcgt gaaaaagccg cttagtttca
     3841 agtattaaga aatattttgt gaaaatggct tctcacattt ttgcataaat ttgacttaat
     3901 accgagaaac tggcttttga acaccgttta ttgagcgtct ataaactgac ctgagtatta
     3961 gagaaacaaa acggcaaagt agctccatat actttgccgt tttgttttga actaataccg
     4021 aagagaagct gatcaaacaa catttctttt ataatataga ttttag
//
LOCUS       DAZUMK010000173         4046 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000173 DAZUMK010000000
VERSION     DAZUMK010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.178"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..439
                     /locus_tag="ACG808_002726"
     CDS             <1..439
                     /locus_tag="ACG808_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048308.1"
                     /translation="LMIKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGN
                     DHSDYRGLNRRCMGILGRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDA
                     VLLNHVRRCSFMSREVGDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(500..865)
                     /locus_tag="ACG808_002727"
     CDS             complement(500..865)
                     /locus_tag="ACG808_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFS5048309.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(941..1186)
                     /locus_tag="ACG808_002728"
     CDS             complement(941..1186)
                     /locus_tag="ACG808_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5048310.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1256..1510)
                     /locus_tag="ACG808_002729"
     CDS             complement(1256..1510)
                     /locus_tag="ACG808_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048311.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1674..1997)
                     /locus_tag="ACG808_002730"
     CDS             complement(1674..1997)
                     /locus_tag="ACG808_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048312.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            2184..2257
                     /locus_tag="ACG808_002731"
     tRNA            2184..2257
                     /locus_tag="ACG808_002731"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2218..2220,aa:Ile,seq:cat)
     gene            complement(2642..3139)
                     /locus_tag="ACG808_002732"
     CDS             complement(2642..3139)
                     /locus_tag="ACG808_002732"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFS5048313.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(3179..3988)
                     /locus_tag="ACG808_002733"
     CDS             complement(3179..3988)
                     /locus_tag="ACG808_002733"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5048314.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
BASE COUNT         1351 a          720 c          710 g         1265 t
ORIGIN      
        1 ccttatgata aaaaaactat ttttactatc aatattaacg ctagcaatgg caggttgttc
       61 tgactctggc ttagcaactg aactgatcac aaaatcaact gaagccacga cagaggagtt
      121 aaaatgtgga aacgatcaca gtgactacag aggcttaaat cgacgatgca tgggaatatt
      181 aggcagaagc aattgtaagc atcgatgtag taatcgaaga aggtgtagaa gaagcaacag
      241 aaaaatcaaa agaaagatca aaagaagcaa tctgtgctgt gcatcgtata cagacgtcga
      301 agatgcggta cttttaaacc acgtcaggag atgttcattt atgtctaggg aagttggcga
      361 tgaaccaaaa ctagagtacg cttttagttt tgcacgtgag gatgaagata accctaaaac
      421 ttacgacatt ttagcttgat ctaagatggt taaagctttt taatgaagta agaggagata
      481 gaaggctttt ctagttatat taggtaagat aaattccttt ttgctccaaa tttgctaaac
      541 attctgctag ataaacttca tcgtgttccg gataaatcgg cacgttgcgc tctacagtag
      601 gtaaatcaaa aaatgcttcg catgatttgc cacgatacat aggatttttc cactctggtg
      661 ctgggctgta accacgtttt tccatttcat ccatgacaag ctgatgatat tggaataatc
      721 tataaggaga atagtgaaaa acataatcca cagtagcatg tttctttccc cagccatttc
      781 ctcttaatgc acagcattcc cgatgttgtc ctaaaagctg ttgacgagga agctttgaaa
      841 tcaatttttc atgccataat cgcatagaaa tgtctccttt atatatagat tgttaacctt
      901 agacgttaaa tgaaaaaacc agcaccaagg ctggaaaaaa tcacttcagt ttgcttgctg
      961 tttctgagcg accaattagg taatcaacgg aaatatcgta aaagtcagcg atatagatta
     1021 attcaatgat gcttggaatt tcttctcctt cttcgtactt acgatatttg aataaaggaa
     1081 tacttaacgc gctagaaaca gctacttgag ataaagatcg gccagttctt aagaaattca
     1141 gacgacaacg taaagtatcc agtaaaatag cttcttcgat actcatttaa tcacctttca
     1201 ttatagtaga atagttataa tgactataga aagctacaca tgcaatcaat agtactcaat
     1261 gttttttatt taaacgtatc aaataaagaa tgacgatcaa taaagacaaa accagtcctg
     1321 ataattcttc tataaaaaca ctatcgggtt caaagtagtt gtaattaaaa actttccaca
     1381 taaagtagat agccggaaga gtcaaaatag ctaaataaaa ccgttctttg ttccaattca
     1441 taagataaac aatacttgcg accagtgcga ccgcaggcac ccaaaacaaa actctcacca
     1501 acgtaatcat gtaacggact cctcaacgat tcatataata aaagataaca tgttattgca
     1561 tacatcacaa atgaaatgct aggaaaataa aagaagaata gatcaatgaa aaatgaaaaa
     1621 gcagaacagt tttctgctga taagcaggaa ctgttccgct gaatgtgtct tatctatcga
     1681 gaaaaaatca atggattgat atctaagtaa ttcaatacat ataaaaagag gacgatcgca
     1741 ttcactaaaa aggaaagaat gatgatccca tagcgaatgg aaacatattt aacaaatact
     1801 tttgtttgaa tacgggcata tctttttcct ttatagctga gcatcatgac aagaagcaaa
     1861 agggtaatca caccttgaaa aatcattcca ataaacagaa ttcctgaggt ttgataattt
     1921 aattggagaa aataagctgc tgccgcaact aaatcgaata aaatcgcaat aaatggcaaa
     1981 ataatcactc ctaacattga gaataaataa aagaataaca ttttttctaa aataagcaaa
     2041 taaaaattaa agtaacaaaa tagttatgac acaagtaaaa gaaataagaa taacaatatt
     2101 attttactta tattttgaaa cttgtgagag tttgtcattc gtgctatagt tgaaaagtaa
     2161 caaaaataaa ttttatttat gatgtacctt tagctcagtt ggttagagca gacggctcat
     2221 aaccgtccgg tcgtaggttc gagtcctaca aggtacataa gaggttgtga aaaagctgcc
     2281 ctgcatcaag taataagaac agccaagtaa aaacagcttc ttatgttttt acttggctgt
     2341 tcttattact gcagatgcaa gcttttgaat accgtttaag gaagaggttg tgaaatcagc
     2401 gttttgacct gagtattagc ttaacaaacg gaaaaatagt tcctcatatt ttgccgtttg
     2461 ttaagctaat accgagggtc agctgattga acaccattta agaaagaggt cgtgagcctg
     2521 ccgtttcact ctgtgtcata aaaaagccaa tctactttga tagaaaaagc acgtaggact
     2581 aaaacagttc tgcgtgcttt ttttattgtg tgttaaatat cgtaaatgaa gatagatatt
     2641 tttatgattc gacgatagag acgttgaaat ctttcaacag ctctaaagcg gattctccgt
     2701 gttttctact ggagactaac attggatcaa caaaaaagtc tgcttcagga aaagcactct
     2761 gcaggcctat cagattagag agtacacaca agtttgtttc aatcccgact acttcgatct
     2821 ctttttgttc ttctttgctt tcaaataaag tttcctggat ttcagctaaa gtttcaggtg
     2881 gaatcgtaaa gtaatacttt ttaatcactc gttcattttc ttgtggagaa agaagaggga
     2941 tcaattgcaa atcttctcgt tcttcttctt tgtccttcag ttcaactggt atatcccgtg
     3001 tgaagagaat atattcatta ttttggcgtg ctttagctaa tctttgagca atacgttctg
     3061 ccaattgatc tgactctgga atataaaatt cgctgtttgg atcaagaata tgattttgca
     3121 tatcaataat aactaacatt ttctttcctc ctacattcat atcgtcattt tattcttttt
     3181 aatagaagtt ttttaaaata taagcaacac cagcttcttc attcgtcagt gttacttggt
     3241 ttgcaatttc ttttacttgt cgctgtgcat tccccatcgc aatactcaac ccgctgtaat
     3301 tcaacatagc aatgtcgttt tcttgatcgc caaaagccat gatgtcactc gattcgattc
     3361 caaggtaatc cgccagaaaa acaagtgaat gagctttagt gatacctgta ggcgtgattt
     3421 ctaagaaatg cggatcagag aatactgatt ggataccatc tgcttgcaga tctctttgta
     3481 gcttctgtag tttttcttca ctcttatctg ttaatgctaa tttaagatag ctaagagaag
     3541 gatcttccaa catatttaaa atttgatcaa aagaatggtt ctgtaaattg aaatgttcat
     3601 aataagggtc atctttgact agatttgatt catcgaatgt cagctgatca tcttgggttt
     3661 ctcggctagc aatgatggat attttcgctt cttgagcata gaagaaggct tttctcagtg
     3721 tatcgctatc tatttttgaa cgtctgagaa ctttcgccat ttttccatcc ttcatttcag
     3781 caatcagtga accattggaa gaaatcaaat agccaccgat tcctaattcg gaaaaaatat
     3841 ctttagcaga aatgctgttc cttccggtag cgacggttac gattccgcca ttttcatcaa
     3901 atttccgaag tgcttggata ttttcttctg gtatctttcc atttcgatca agcaaggtac
     3961 cgtctaaatc aatcgctgct agtttcattt tatcttccta ctttcttttt acatatctat
     4021 tttcctgtta ccttaagatt aaatga
//
LOCUS       DAZUMK010000174         4046 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000174 DAZUMK010000000
VERSION     DAZUMK010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.179"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            151..954
                     /locus_tag="ACG808_002734"
     CDS             151..954
                     /locus_tag="ACG808_002734"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS5048315.1"
                     /translation="MLLSLYDKEKPEYLKEALTSIFEQTVMPKEIVLVYDGPINEKLQ
                     VVVTYFQQKDPHLFTVVKLEKNQGLGIALAIGLEHTKNRLVARMDTDDIMEPQRLEKQ
                     LEIFQKDPEIAIVGSNIEEFTGTLSNVIGKRIVPEYNNAIRVFSKRRNPFNHMTVMFD
                     KKAILEVGNYQPLSGFEDYYLWARLLKAGYKGYNIQETLVYARTGKDMYARRGGMKYL
                     GSGLMGRYRIWKDGLGSVKDFIIVSCGHIFISLLPNQLRGKFYQTQLRK"
     gene            987..1841
                     /locus_tag="ACG808_002735"
     CDS             987..1841
                     /locus_tag="ACG808_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HFS5048316.1"
                     /translation="MKKIQQITLKMSQEIVDFCLKNQLLCYFCGGGAIGTVREQGFIP
                     WDDDLDFFMPREDYEKFYQLWVNSEKSKKYPIQKASKNYNDHNSFTTIRDADTTFIKT
                     YQRDLDIVHGITIDIFPLDVAPVSKFSRKIQKLWALVYALFCSQVVPEKHGGIMATGS
                     KILLSIFRSPRTRYKIWSFAEKQMTKYNNEPTGYVTELCVGPKYMGNVYPAKDFESAV
                     WVPFEDTEMPVPVGYDNYLSQVFGDYMQLPPEKDQVSHHEAVYIDPEHPYTMYKGKYY
                     LTKGAEKK"
     gene            1838..2539
                     /locus_tag="ACG808_002736"
     CDS             1838..2539
                     /locus_tag="ACG808_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFS5048317.1"
                     /translation="MITAIIIAGGVGKRMGQDIPKQFITIEGKPIIIYTLESFQHHPQ
                     VDRILVVCKSGWEETMWAYIKEFNISKVKWVITGGSKGQESINNGVQFLKDYSEDDDT
                     IIIHDGIRPLVDELVLSDVIVKCKEFGNGVTSLPYNEQIFIKKTEETTEQYVDRNTLR
                     RVSTPQAYQYGKLRAAYDRAVKEDIGMTDSSYTNTMMVDLGETLYFAAGSDKNIKLTT
                     TDDLELFKAYLKMKD"
     gene            2547..3614
                     /locus_tag="ACG808_002737"
     CDS             2547..3614
                     /locus_tag="ACG808_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFS5048318.1"
                     /translation="MMTLIKQPMYQEDLAKVINHTPKIKQLHGKSVLMIGASGMIGSF
                     LIDTLMAANEQLGIKIKVYAMGRNRQKLEKRFASYLELDTFEIVEGDVTEPLPKEIQA
                     DYLIHGASNTHPKAYATDPIGTIMTNLAGTEQVLKHAVATQPVRVLFLSTVEIYGENR
                     GDIEKFTEDYCGYIDCNTLRAGYPEGKRVSESLCQAYIAKHDLDIVIPRLCRTFGPTM
                     LLSDTKASSQFILNAVEQKDIVLKSEGNQYFSYVYVGDAVSAILHLLLEGEKGEAYNV
                     SSENFDLRLKELAQQLADISGKQVIFELPDEIEQKGFSKASTAVLENKKIKETGWKPI
                     FPLNESLVHTVELVKGEKYNV"
     gene            3607..>4046
                     /locus_tag="ACG808_002738"
     CDS             3607..>4046
                     /locus_tag="ACG808_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS5048319.1"
                     /translation="MCEISIIVPVYKVEKYLRKCVDSILAQTFTDFEVILVDDGSPDN
                     SGKICDEYAEKDNRVRVIHKENGGLSSARNAGIDVARGKYLGFVDSDDYIDEDMYEIL
                     YENLKIHDADISSVELIPFYGDRYKKANKEKKVIILNKKEAIKSV"
BASE COUNT         1457 a          589 c          807 g         1193 t
ORIGIN      
        1 ttgataagat gtcatctcaa ggactagatg aagatgaaaa tattagttca ttagcagaaa
       61 tcgacttttc acaagagaaa atttatcgct agattggaaa aattttgagc aaagaggtag
      121 gagagaacat tggaaaataa aaatgtaagt atgctgcttt ctttatatga taaagaaaag
      181 ccagagtatt taaaggaagc attaactagt atttttgagc aaacagtaat gccaaaagaa
      241 attgtattag tttatgacgg ccctataaat gaaaaacttc aagtagttgt cacttatttc
      301 caacaaaaag atcctcactt gtttacagtt gtaaaattag aaaaaaatca aggattgggg
      361 attgctttag caattggatt agaacatacg aaaaatcgct tagtagccag aatggataca
      421 gatgatataa tggaacccca acgattagaa aaacaattag agatcttcca aaaagatcca
      481 gaaatagcga ttgttgggag taatattgag gaatttactg gcacgcttag caatgttatc
      541 ggaaaaagaa ttgtacctga atataacaat gcaattcgtg tattttctaa aagaagaaat
      601 ccctttaatc atatgaccgt tatgtttgat aaaaaagcta ttttggaagt gggaaattac
      661 caaccattaa gtgggtttga agattattat ctttgggcaa gattattaaa ggcaggttat
      721 aaaggatata atattcaaga aacattagtc tatgcgcgaa ctggcaaaga tatgtatgca
      781 agaagaggcg gaatgaaata tcttggttct ggtcttatgg gtcgttatcg tatctggaaa
      841 gacggtttag ggtcagtaaa agattttata attgtttctt gcggacatat ttttattagc
      901 ttactaccaa atcaattaag aggaaaattt tatcaaacgc aattaagaaa ataggaggtt
      961 tttctttgta tagcactgat ccaaaaatga aaaaaataca acagattacg ttgaaaatgt
     1021 ctcaagagat tgttgatttt tgtctaaaaa atcaacttct ttgttatttc tgcggtggtg
     1081 gtgccatcgg tactgtgaga gaacaagggt ttattccatg ggatgatgat ttagatttct
     1141 ttatgcctag agaggattat gaaaaatttt atcaactatg ggttaattca gaaaaaagta
     1201 aaaaatatcc catacagaag gcttctaaaa attataatga tcataattct tttacaacta
     1261 tccgcgatgc agatactaca tttattaaaa cttatcaaag agatttagat atagtccatg
     1321 gaattactat tgatattttc ccgttagatg ttgcccctgt ttcgaaattt agccgtaaaa
     1381 ttcaaaaact atgggcttta gtatatgctt tattttgttc tcaagtcgtc cctgaaaaac
     1441 atggtggaat aatggctaca ggtagtaaga tactcttatc tattttccgc tcacctcgta
     1501 cgagatataa gatatggtct tttgctgaaa aacaaatgac aaaatataat aatgaaccaa
     1561 cgggctatgt gactgaactt tgtgtcggac ctaaatacat gggaaatgtt tatcctgcaa
     1621 aagactttga atctgctgtg tgggtgccat tcgaagatac agagatgcca gtaccagttg
     1681 gttatgataa ttatttaagt caagtttttg gtgactatat gcagcttccg cctgaaaaag
     1741 atcaggtttc tcatcatgaa gcagtttata ttgatccaga acatccatac acaatgtaca
     1801 aaggaaaata ttatttaact aaaggagcag aaaaaaaatg attacagcta ttattattgc
     1861 tggtggtgtg ggtaaacgaa tgggacaaga tatcccaaaa cagttcatta caattgaagg
     1921 aaaacctatt attatctata ctttagaatc atttcaacac catcctcaag ttgaccggat
     1981 tttagtcgtt tgcaaatctg gttgggaaga aacgatgtgg gcgtatatca aagaattcaa
     2041 tattagcaaa gtcaaatggg tcattacagg tggatcaaag ggacaagagt cgatcaataa
     2101 tggtgttcag tttttgaaag actattctga agatgatgat acgatcatca tccatgatgg
     2161 aatccgtcca ctagtagacg aactggtatt gtcagatgta atcgttaaat gtaaagaatt
     2221 tggtaacggt gtaacctctc taccttacaa cgaacaaatt tttatcaaga aaacagaaga
     2281 aacgacggaa cagtatgtcg atcgtaacac attgaggcgt gtctcaactc cacaagctta
     2341 tcaatacgga aaactacggg cagcatatga ccgggcagtg aaagaagaca tcggtatgac
     2401 tgattcttct tatacgaata ccatgatggt agatttggga gaaactttat attttgctgc
     2461 tggttctgat aaaaatatca aattgacaac aacagatgat ctagaattgt tcaaagcata
     2521 tttaaaaatg aaagactagg tgaaagatga tgacgttgat taaacaacca atgtaccaag
     2581 aagatttggc aaaagtaatc aatcatacac caaaaataaa acagctacat ggaaaatccg
     2641 ttctgatgat tggtgcttct ggaatgatcg gctctttttt gattgatacg ttgatggctg
     2701 ctaatgaaca actaggcatc aaaatcaaag tttatgcaat gggcagaaac cgtcagaagc
     2761 ttgaaaaaag gtttgccagt tatcttgaat tagatacttt cgaaattgtt gaaggtgacg
     2821 tcacagaacc cttaccaaaa gaaattcaag ctgactattt gatacacggt gcaagtaaca
     2881 cacatcctaa ggcttacgca actgatccaa tcggtacgat catgactaat cttgcaggta
     2941 cagagcaagt attgaaacat gcagtagcaa cccaaccggt tagagtatta tttttatcaa
     3001 cagtagaaat ctatggcgaa aatcgaggcg atatagaaaa attcacagaa gattactgtg
     3061 gatacatcga ttgtaataca ctgagagcag gctaccctga aggaaaaaga gtcagtgaat
     3121 cattatgcca agcctacatc gcaaaacacg acctcgacat cgtgattcca aggctttgcc
     3181 gtacgtttgg tccgacgatg cttttatctg atacgaaagc atcctcacaa tttattttaa
     3241 atgcagtcga acaaaaagat attgttttaa aaagtgaagg aaaccaatat ttttcgtatg
     3301 tatatgttgg agatgctgtt tctgctattc ttcatttact gcttgaagga gaaaaaggag
     3361 aggcgtacaa cgtttcttca gaaaactttg atttacgact aaaagaatta gcccaacagc
     3421 tagctgatat cagcggcaaa caagtaattt ttgaattgcc ggatgagatt gaacaaaaag
     3481 gtttctcaaa agctagcaca gcagtacttg aaaataaaaa aattaaagag acaggttgga
     3541 aaccaatttt tcctttgaac gaatcattgg ttcatacagt agaattagtt aaaggtgaga
     3601 aatataatgt gtgagattag tattattgtt cctgtgtata aagttgaaaa atatttaaga
     3661 aaatgtgtag attcgatttt agcccaaaca tttactgatt ttgaagttat attagtagat
     3721 gatggatctc ctgacaatag cgggaaaatc tgtgatgagt atgctgaaaa agataatcga
     3781 gtacgtgtga tccataaaga aaacggcgga ttaagcagtg ctagaaatgc ggggattgat
     3841 gttgcgagag gcaaatactt aggctttgtt gacagtgatg attatattga tgaagatatg
     3901 tatgaaattt tgtatgagaa tttgaaaatt catgatgccg acattagctc tgttgaattg
     3961 attccttttt atggtgatag atataagaaa gctaataaag aaaagaaagt aattatatta
     4021 aataaaaagg aagcaataaa atctgt
//
LOCUS       DAZUMK010000175         4045 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000175 DAZUMK010000000
VERSION     DAZUMK010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.180"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            232..1107
                     /locus_tag="ACG808_002739"
     CDS             232..1107
                     /locus_tag="ACG808_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS5048320.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            1211..1975
                     /locus_tag="ACG808_002740"
     CDS             1211..1975
                     /locus_tag="ACG808_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFS5048321.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            1962..3404
                     /locus_tag="ACG808_002741"
     CDS             1962..3404
                     /locus_tag="ACG808_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5048322.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            3415..>4045
                     /locus_tag="ACG808_002742"
     CDS             3415..>4045
                     /locus_tag="ACG808_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFS5048323.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIY"
BASE COUNT         1331 a          590 c          855 g         1269 t
ORIGIN      
        1 ttatagagag ttgctatatt ttttttgttt ttatgtaatt acctccaaat caattcgata
       61 caagtattat cataaaacga gtaaaaaaga tattgataac gcttttatta tgtgttaaac
      121 tatttttgta aattatgtag ggtgctacta ttcaattagg cacgacctaa gaaagggttc
      181 tttcttaggt cgttttttgt ctttaaaaat tgtttggaga caaaaatatg tatgaaggag
      241 tctgcaagtt gttttgtttt atcatataga ggaggtgaat taacggtgta tgtgatgaaa
      301 aagctgaatc ataatgctgt attgatcgat gacgatggaa tagaaaaaat tgcgatgggg
      361 aaagggattg gcttttctgc taaagtgagt aagccatttg atgttgagga tgctgacaaa
      421 ttatttgtat tagattctaa agaaaagatc aaacgattca gtgagatagt ctcgcagatt
      481 ccaatcgaat ttatggattt tgcggaagaa gtgattgaac tgataacagc taggatctcc
      541 tctccattag attcaaatat ctatattaca ttgacagatc atatttattt tgctattcaa
      601 cgaaaaagag aagatagaga tgtcacagca attatgttgc cagaaatgag attcctttat
      661 ccaaatgaat atgatatagc agtagaagtg gtcgagcgaa tcaatcatcg atatcaaacc
      721 gagttagggt caaatgaagt aggattcatc actatgcata tcattaatgc tgagctagga
      781 gagcctaata gtttaaagag cttaaaaata gtagaaatga cgaccacgat tttgtcccat
      841 ctagaaaaat cctattcaaa cgtttatgat aaagaatcac tgacttatca tcgcttaatg
      901 gttcatatca aattcctggt taaacgcttg atttatcagg aagaaacgcc agtagaaaat
      961 attggttttt ttactgaaac atttaaacat agcaaagcgt attgtacagc actaaagatt
     1021 aaagatatca tcgaacagca atttcaactt gccattcaag aaaatgaaat catctatttg
     1081 gcaattcatc tagcaagaat ctattaaaat aagggcgtaa ctatctatag gcgcaaccaa
     1141 atgacacata gtaaatggac tatgattatt tggttgcgcc tattttagta gaaaaaagaa
     1201 ggagaatgaa atgggaaaag tgatttttaa ttcggatgat tttggctata gccatggtgt
     1261 gaattatggg attatggatg cttatcagcg aggaattttg acatcaacta cattgatggc
     1321 aaatatgccg ggtttcgagc atgctgtgaa actaagaaag gaaatgccta ggctaggtgt
     1381 aggagtccat ttaacattga cgtgtggcaa accactcctc aaaactgtcg atactcttat
     1441 cgaaagcgga gagttcagat ccttatctta ttatcagcat ccatttaaca ttgacaaaga
     1501 tcaactgtat caggaatgga atgctcagat tcaaaaaatt tatcatgcgg ggatcatacc
     1561 aactcattta gacagtcatc atcatactca tactttggat gaaaatcagg aggtagttgt
     1621 tacattagca aaaaaatatg atttgccggt tcggagcaac tttgaacgaa aagatgaagt
     1681 tcgtcatgtc gattattttg agccttgttt cgatgtcgta ggagaaactg atgaaaaaaa
     1741 acgacaaatc aatacgagtg tggaagaata tttggcaagt ttactcatga tattgagaga
     1801 agataaaacg atcgaagtca tgtgtcacgc agcgtatttg gatcaggaat tgttaaatgg
     1861 ttcaagtttt gtgtatcctc gtacccacca agtcgaattt ttaattcatt cagcatttgc
     1921 tcaaaaagtg aaaaatgatc ctttgattga gttagtaaca tatgaggata tttaatagag
     1981 gaggaataaa aatgaatgtt aaatcaaatg tgcagaagct tggaagagcg atgctacttc
     2041 cagttgcagc gatgccatta gcgggattga tcatgcgttt atcagctgac gatatgctga
     2101 acatcccagt aattggtgca gctggaaatg ccgtttttgg aaacttggat atcttatttg
     2161 ctataggagt tgcaatcggt tttgtcaaaa ataaggacaa aggaatacct gcacttactg
     2221 gttttttagc gattgctacc ttgaaaaaag gcttagaaat tatggaccca gaagtgaata
     2281 tggggatttt tggtggaatt atttccggtt tagtagcggc atggacgtat aatcgcttta
     2341 aagaacaaaa attaccagta gttttttctt attttgcggg cgaaaaattc ccattgacta
     2401 tggtgatgat tctacaaaca atcgcagctg tttttttggg ctttatctgg ccgctcatcc
     2461 aaattgggat tgataatttt gcaaaactgc tcgttgatat gggagcatta ggtgtgggga
     2521 tatttatgtt tttgaatcgt ttgctgatcc cctttggact gcaccatgtg ttgaatacgt
     2581 atatttatta cgatttagga agctatacat ctcctagcaa ggaagttttc agaggagaga
     2641 tgacacgctt tatcaacgga gatcctcagg ctggactgtt tttatcaggt ttttttgtag
     2701 taatgatgtt tggtatacca gctatatgtc tagctatcta taaagcagct tataaagaaa
     2761 ataaagagat ggtcaaagga atcatgggaa gcggtgcagg aacttcattt attgcaaata
     2821 tcactgagcc tgttgaattt agtttcatgt ttctttctcc tatgttgtac gtgatccatg
     2881 cactttttgc cggtttagca gggattgtct gttacttatt caatatacga attggtttta
     2941 cattcggagc atgtattgtt gattatctca tcaattttcg aatcgctacc aatgctattt
     3001 tgattcttcc aatcggaatt ttcttttttg ccttgtatta cgttactttt tattatttga
     3061 tcaataagag aaacattcag acattaggac gtgaagctaa ggcagagttt ggcaatgaag
     3121 taaccttaga agaaacagag ttagggttag caagtaaaaa ttatgaatat atggcaacaa
     3181 aaatgctaca agcgtttgga ggaaaagcca atatcctgga tgtctatagt tgtaatacac
     3241 gcctacgggt agaggtagtg gatcccacca tggtcgagga acagcgaatc aagcaattag
     3301 ggataagcgg gatcattaaa ccaactgaaa aaaattatca aattatcatc ggcttagaag
     3361 tgacttatgt tatggcagaa ttcaataaat tattagaaga atagaaagga aaagatgatg
     3421 atgaaaaaac agattatcac tatagctgga ggcgggagta cgtatacacc aggaatcgtt
     3481 caagccattt tgaacaatga aaaacggttg ccattatctg aaatacgttt atatgatatt
     3541 gatgaagaaa gaaatatgga tatgtacttg attgttaaat ttatgctgaa aagaaaaggt
     3601 ttttcaaata ttcgcattcg tgcaacggat gatccaaagc ttgcttttac aggatgcgat
     3661 tttgttttct cacaaattcg tgttggagga ttggagatgc gagaaaagga cgaaaagatt
     3721 ccacttaaac atggattagt aggacaagaa acgtgtggac tcggaggatt tgcttatggg
     3781 atgcgctcaa tcaaaggttt attggaaatt gtcgatcata tacaagacta tgcccccaaa
     3841 gcatggattt tgaattatac aaatccagag tcgatcgttt ctgaagctgt tcgtcgtaaa
     3901 tatccgaatg taaaaatgat taatgcctgt gatatgacta tttcaattga agaaacgatt
     3961 gcagtgaatt acggttatga tcgtaagaat tggatcgtta cgtattatgg tttaaaccac
     4021 tttggctggt atacatcgat ttatg
//
LOCUS       DAZUMK010000176         3891 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000176 DAZUMK010000000
VERSION     DAZUMK010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.181"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..2130
                     /locus_tag="ACG808_002743"
     CDS             <1..2130
                     /locus_tag="ACG808_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFS5048324.1"
                     /translation="HKVTFTAGEGTSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTD
                     AKWPEEATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNK
                     EDLPKDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPT
                     VEGEKVEVGGTVDLTDNVTNLPTLPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDG
                     TKDTVKVPVEVTDNRSDADKYEPTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVT
                     PGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGG
                     TVDLTDNVTNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVE
                     VTDNRSNADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNK
                     KGTIVVTYPDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKED
                     LPKGTTYTWKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPIT
                     EGDQIVTGKTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESG
                     NTSEPTNVVVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSN
                     IFTISGGLILLGTLGLLGYKNRKKENE"
     gene            2264..3607
                     /locus_tag="ACG808_002744"
     CDS             2264..3607
                     /locus_tag="ACG808_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS5048325.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            3719..>3891
                     /locus_tag="ACG808_002745"
     CDS             3719..>3891
                     /locus_tag="ACG808_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFS5048326.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSEL"
BASE COUNT         1544 a          558 c          771 g         1018 t
ORIGIN      
        1 cacaaagtaa ccttcaccgc aggagaagga acaagtattg aaagtggaac aacagtcttt
       61 gcagtgaaag acggcgtaag cttaccagaa gataaacttc cagtgttgaa agcaaaagat
      121 ggttatacag atgcgaaatg gccagaggaa gcaactcaac caattacagc agatgataca
      181 gaatttgtat caagtgcaac aaaactggat gataaatctg acgctgacaa atataatcct
      241 gaaggtcaaa aagtgactac agaattgaat aaggaaccag acgcatttga gggaattaag
      301 aacaaagaag atttaccaaa agatactaag tatacttgga aagaaaaagt agatgttagc
      361 gcagctggaa ataaaaaagg gactgttgta gtgacatatc cagatggatc atctgatgaa
      421 gttgaagtag atgttacagt gacagacaat cgttctgacg ccgataaata tgagccaaca
      481 gtagaaggcg aaaaagtaga agttggaggc acagtagatc taacagataa tgtaaccaac
      541 ctaccaacat taccagaagg aacaacagta acagatgtca ctcctggtgg tacgattgat
      601 accaacacac caggtaatta cgaaggggtt atcgaagtaa cgtatccaga tggtacgaaa
      661 gatacagtga aagttccagt agaagtgaca gacaatcgtt ccgatgccga taaatatgaa
      721 ccaacagtag aaggcgaaaa agtagaagtt gggggcacag tagatctaac agataatgtt
      781 actaacttgc cagcgttacc agaaggaaca acagtaacag atgttactcc cggcggtaca
      841 attgatacca acacaccagg taattacgaa ggtgtcattg aagtaacgta tccagacggt
      901 acgaaagata cggtgaaagt tccagtagaa gtgacagaca atcgttctga cgccgataaa
      961 tatgaaccaa cagtagaagg cgaaaaagta gaaattggag gcacagtaga tttaacagat
     1021 aatgttacta acttgccagc gttaccagaa ggaacaacag taacagatgt tactcccggc
     1081 ggtacaattg ataccaacac accaggcaat tatgaaggtg tcattgaagt aacgtatcca
     1141 gacggtacga aagatacggt gaaagttcca gtagaagtga cagacaatcg ttctaatgca
     1201 gataaataca atcctgaagg acaaaaagtg actacagact taaataaaga gccagatgca
     1261 tctgagggaa ttaagaacaa agaagattta ccaaaaggaa ctacttatgc ttggaaagag
     1321 aaagtagatg ttagcacagc tggaaataaa aaaggtacta ttgtagtgac atatccagat
     1381 ggatcatctg atgaagttga agtagacgtt acagtgacag atactcgttc cgatgcagat
     1441 aaatataatc ctgaaggaca aaaagtgact acagacttaa ataaagagcc agatgcatct
     1501 gagggaatta agaacaaaga agatctacca aaaggaacta cttatacttg gaaagaaaaa
     1561 gtagatgtta gtacagctgg aaacaaaaaa ggtactgtag tagtaacata tccagatgga
     1621 tctaaagaag aagtagaagt tactatttct gtagtagata aaaaagcacc gaataaacct
     1681 caagttgatc ctattacaga gggtgaccaa attgttactg gtaaaactga accaaatgca
     1741 gaggtgacag ttacattacc agacggaagt caacaccatg ggacagctga taaaaatggt
     1801 aattttacag ttaaagttcc taaattagag gcagggacaa aagtgatagt aactgcaact
     1861 gatgaatctg gtaatactag tgaacctact aatgtagttg tttcttccaa caaaaaagac
     1921 agtggaaaaa acggtagtaa aggtagtaag actgacaatc aaggtagcaa cagcaatcaa
     1981 gacaaaaata gaggcaaaag ccagtcctca aaagtttttc ctaagacggg agagagtgac
     2041 agtaatatct ttactattag cggtggttta atactgttag gaacgttagg attattaggg
     2101 tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt tcttaagtct tctctgagaa
     2161 gaggaagaat ctttttcttc ctctttttgc ttttttatga tgggaaataa gtgttagtta
     2221 aagatgaata tactttttat agaaaggaaa agaatgtaat taagtggaaa aaatagtaat
     2281 tattggttca aaccattcag ggctagccgc agctcgattt ctaaacttaa gcgattcaaa
     2341 ttatgaagtt attctaatcg ataaaaaatc agaaattagt tacttggaat gtgggacatc
     2401 gttatttata aaaggagaag taagtaattt agaagatctt ttttatcaaa atagaatgac
     2461 actaaattct gagataaaaa agttatttct agagacagaa gttgacaaaa ttgatattca
     2521 aaataaaaag attagttgtg taaaaaaaga tggtacaaga ttctctcaag aatatgacaa
     2581 agttatatta gctacaggtt caaagcaaat tatgctagat ataccgggga ataatttagc
     2641 taatattttt tcgataaaga attttccaaa tgcaaaagag atttttaaag ctttggatga
     2701 tagtactatt aaaaaagtag gaattattgg agcaggatat attggagttg aaattgctga
     2761 agccataaga aaaagaggga aagaagtata tcttttcgat gttgcagata gagtgttgtc
     2821 gacttattac gaccgtagtt tttcagataa agttgaagaa atactatcaa aaaatggaat
     2881 acatctttgt ctatcagaaa gtcctaaaaa atatttagga agaaaaaaga ttgaaaaaat
     2941 agtcactaca agtggtaccc aatactctat ggatatggtc gtacaagcta taggatttct
     3001 atctaatagt ccaattgggg aaaaggaact cttgagagat gttagtggag catatctaac
     3061 taatgaatac cagcaaacaa atataaaaga tatatatgct attggggatt gtgctacaac
     3121 tttttttacg tccattaata gaagtagtgt tgatttttct atttctaatg ctctaagaac
     3181 agcttatatt gcaactcaac atattaacaa tcaagatttt acaccttctg gttcccaatt
     3241 tacaaatggc ttttctatat ttaattacaa tttttacgca gcaggtttga atttaaaaac
     3301 agcacgatta ttaagtgagt ctatagatta tatagaaata gaagatttca ttagacctgc
     3361 ttttttgaga aaaaatagtc gtgtaaaatt aagaattagc tttgaaaaaa atacaaagag
     3421 aatagtaggt gtacaacttt gtagtcaaga agatttatct ggactattat caatgttttc
     3481 tcttgctata gaagaacaaa ttacattaga caaattgaaa atgttagatt atttatttca
     3541 tcctagtttt agccaaccct ataattttat tttaaaggca gtaacaggat taaacattga
     3601 aacataatcg ttactaaatg aaatcaagaa atatactgta ttactcaatt cattgaaaat
     3661 atttatcata atttttaata gaaaacacta ttaaagagaa aaaagagagg taaatcaaat
     3721 gaaaaaatct ttaaaacttg catcagcagt tatattaatg tcccaactcc ttatagcaac
     3781 acctttccat gtgttggcaa gcgaaaattc aacatacaaa attgcaaaag aaagaaccga
     3841 gacgactgaa atgagtaatt ccgatgtaac agcagagagt agtgaacttt t
//
LOCUS       DAZUMK010000177         3785 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000177 DAZUMK010000000
VERSION     DAZUMK010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.182"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..168
                     /locus_tag="ACG808_002746"
                     /pseudo
     CDS             1..168
                     /locus_tag="ACG808_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            406..642
                     /locus_tag="ACG808_002747"
     CDS             406..642
                     /locus_tag="ACG808_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048327.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            649..2601
                     /locus_tag="ACG808_002748"
     CDS             649..2601
                     /locus_tag="ACG808_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFS5048328.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2673..3170)
                     /gene="dfrG"
                     /locus_tag="ACG808_002749"
     CDS             complement(2673..3170)
                     /gene="dfrG"
                     /locus_tag="ACG808_002749"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFS5048329.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3445..3785
                     /locus_tag="ACG808_002750"
                     /pseudo
     CDS             3445..3785
                     /locus_tag="ACG808_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1344 a          614 c          655 g         1172 t
ORIGIN      
        1 tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata
       61 gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga
      121 aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatta agtagttcag
      181 ttttggagta caaaaagtga aaatgtaaac ctaaatgagt atcaaattgc aactgaattt
      241 gatactcgtt ttttattgtt aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag
      301 ttaaaatgta tataaaaatg cgaacgtgaa ataattattg taggaatgca aattggtgtg
      361 ttaaaatggt tacaatttac taagcaaaaa ggggtgcatt ttcctatgca atttcttgag
      421 ccaaaaaata agaacgctaa gtctgtggat tgggaaatat cagagcaagt aagggtgata
      481 gttaagcaat acgctgaata tgcagagcgt actgaaagcg aagcagtgga tgaattctta
      541 ttgaacattt tagacgataa gaagtttatt gaatggattg ccaacaagcg aagtaataag
      601 cggattgttg aaaaaatggg tatcaaagat agagtgggtt gatagacaat gaccaagctt
      661 aaaagattag ccacgaagga agatgaaatt gtagatgtta aaattccgat ttcaaacgaa
      721 gaattaaaag atagggctaa acaatatgat ttattaactc caaaacgatt tgcaacaagg
      781 tataacaaaa tgttgttctt gccaacgtct tttaaatgga acggttctga atatcccatt
      841 caatataatt actgcatcaa tccgttttgt tgtaattttg ggaaagaaca gcacaaattc
      901 aaagatgtaa aagggaaacc aagtcgatat aaaatgactg gtagttccaa agataaaggt
      961 cataaaggta tgtactgcaa tgacaatcct attggcagag gtgtatcgca aaactgtaca
     1021 gtaacccctt tatctaattg gtctgttgtt gaagaaatta aacgccttat agaaatcaat
     1081 agcatccaag atgttgagcc agattatcaa tttcataaag aaggctgctc ggaagaagaa
     1141 tcaaccccat tcaatgagcc gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct
     1201 caacgctatc aatgtaaggc gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa
     1261 accactactt atcaccaaca aaagaacacc attcttccaa tgttcgctaa gatggttgtt
     1321 ggaagggttt cagtcagtcg tacttgtgat attttaggaa ttggagttgg aacctactat
     1381 cataagctgg agtggctgta taggcgttgt ttggagtttt tagaacgata tgagacacaa
     1441 ccccttcaaa caaagaaatt taacgagatg tggcttaata cggacaagat gcactactac
     1501 ctaaataacg tccgtaagaa aggacaaggt tcaaagaaat atacggggtt tgaagacctt
     1561 aatatgcaaa cgtatatagt tgtatctgct gaggtcttat caagatatgt gttccgttct
     1621 gatgttgcct atgattggaa tatttcaatg gatgaattaa atgaagatac aagaaaattc
     1681 aaagaagacc atctcaatac ttttagtcgt aaaaatgata ggttagattg gtcttattat
     1741 ccacaagaac catccgctaa cgatagcgaa aataggaatg cttatcttca cgaattaggg
     1801 aaaattacta ataggagtag atttgtcgat ggtttaaatg ttgatgctcc ttatactaca
     1861 actgctcact attggttaat taaacaaatg gttaatgccg acgaatggcg aatgattagt
     1921 gatgatgact tctctattag aaacgccttt tatagagtat tcacgaaaga attaaggttg
     1981 agcgatgcac atcattttat ttgccaagtt aacaagacga aaagcagaaa acaatgctta
     2041 aaagaattcg gacaagcaaa agcagaactg ctcgattggg gagatatcag aggattcaaa
     2101 acaaagtttc tacggacttt ggcatctcat tatctaactg aattactaac aagtcatcaa
     2161 ttccacgaag aggctattag taaagatgga gaaagatatc gcaaatatgc cgataaccct
     2221 atcaaacatc cattagccac taaggataaa ggattttatt cagttgattg tagaacggat
     2281 ttatctgctc tggaaccaaa cgaaatcgca aagatgctat taaatgttaa tgaccattcg
     2341 accaatagct ttattcaaca gataagacgt tatatatcat ctcttgaaag accattaaca
     2401 acagctcgtg gtgataagaa gagctatatt tacgctaact tcaatccgaa gtatgctcaa
     2461 tttgcgatta caattctaag aacttactac aacttttgtc gcccttttaa atcagcggat
     2521 aaaaaggttt tgacacctgc tcaaagatta gggattacag ataaacaatt tgattggaaa
     2581 gatataattt atttcaaatg acaaaaagag cttggagtaa tcccaagctc ttgtgcttta
     2641 tctttactgt tcttcttcaa ttaacgcacc cgttaactca ataagttttt tctttcatat
     2701 acatgaaaat agtagttata cggattttta tcattcttta tccctttttg ggcaaatacc
     2761 tcattccatt cctcataatt cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt
     2821 atttttgtga tgtacatttt ctcaacataa gggaaaaaca aattataaat ctgttctcct
     2881 ccgaaaataa aaatttcttc ttcgttttta cataactcaa aaacatcttc tattgaatga
     2941 acaatttcac aaccattaaa ggtaaacccc ttatctctcg tcagaataat atttcttctg
     3001 tcaggtaagg ctcttccgat tgattcaagg ttcttcctac ctaatattat cggatgtccc
     3061 tttgtagtat ttttaacata ttcccagtcc ttgggaatcc tccaaggaat gtcattctct
     3121 ttgccaatca ctctattctt atccatcgca gcaatcaaag aaactttcat ttataacaca
     3181 gcctttttaa ctaatttacc atctaatttc ttatttctat caaaaaacct tttctcatta
     3241 tcctgcccgt tagttgaata agcaattttt tctataagtt atattatcat aataccacaa
     3301 aatcacaaat tttcattcaa ttatcatttt caattgcatt ttataaatca ttttgtatgt
     3361 aatttggtat gtaagttaaa atgaaaaaag tagtccattt tgaagtacat tttgtcattt
     3421 tgtactccaa aactgaacta cttacttgca tatatacgcc tttatagtgg agtactacat
     3481 ttacgagatt cggttagagt atcagaaaaa gaaaaaataa aagttacaga aatgtatact
     3541 tcaataaatg gtgaattatg taagattgat agagcttatt ctggagaaat tgttattttg
     3601 caaaatgagt ttttgaagtt aaatagtgtt cttggagata caaaactatt gccacagaga
     3661 aaaaagattg aaaatccgca ccctctacta caaacaactg ttgaaccgag taaacctgaa
     3721 cagagagaaa tgttgcttga tgcccttttg gaaatctcag atagtgatcc gcttctacga
     3781 tatta
//
LOCUS       DAZUMK010000178         3776 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000178 DAZUMK010000000
VERSION     DAZUMK010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.183"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..579
                     /locus_tag="ACG808_002751"
     CDS             127..579
                     /locus_tag="ACG808_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048330.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="ACG808_002752"
     CDS             593..1282
                     /locus_tag="ACG808_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048331.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="ACG808_002753"
     CDS             1407..3758
                     /locus_tag="ACG808_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFS5048332.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
BASE COUNT         1282 a          494 c          777 g         1223 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaa
//
LOCUS       DAZUMK010000179         3704 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000179 DAZUMK010000000
VERSION     DAZUMK010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.184"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1466
                     /locus_tag="ACG808_002754"
     CDS             <1..1466
                     /locus_tag="ACG808_002754"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFS5048333.1"
                     /translation="QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDIL
                     GYAIAPEYLFNVLADQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTD
                     EQQRNVTITEVIKKLNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSD
                     MMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAK
                     MNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRF
                     NRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSI
                     YAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKI
                     LETYAERKDVEKYAHLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQ
                     EQQVLEKKLLEAISSLQTTPENEAWLQGALEVFKHEQ"
     gene            1456..2673
                     /locus_tag="ACG808_002755"
     CDS             1456..2673
                     /locus_tag="ACG808_002755"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFS5048334.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            2686..>3704
                     /locus_tag="ACG808_002756"
     CDS             2686..>3704
                     /locus_tag="ACG808_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HFS5048335.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFA"
BASE COUNT         1278 a          632 c          767 g         1027 t
ORIGIN      
        1 aacaagtggt tcttttagca gatgaatcct tagaggaata tgacacagta agcaaacaaa
       61 caatgttgta tcgtgaatta ctttcagatg aagaatcaaa agaagatctg attgccacaa
      121 tcgttgatat tttgggatat gcaattgcac cagagtatct tttcaacgtc ttagcggatc
      181 aagcaaaaca agcaacgttc caattgaacg atctaaacaa agcctttgtg caattggcat
      241 ctacctacaa tcaatttaat gggttatttg atgatgtgga tctccaatca aaaaaactag
      301 gaacagatga gcaacaacga aatgtgacga ttaccgaagt catcaagaaa ttgaatgatg
      361 tagatgtttt agaacacgat ggggacgtga tcggggatgc gtatgagttt ctgatcagtc
      421 aatttgcttc agaagcaggg aagaaagccg gagaatttta cacacctcat atggtttctg
      481 atatgatggc gcaaatcgta acgctcgatc aaaaggaacg acgattcttt agtgtttttg
      541 atccaacgat gggttcaggt tcattgatgt taaacgtacg taactactta acacacccag
      601 ataacgttaa atatcatggg caagaactaa atacaacgac atacaatcta gcaaaaatga
      661 acttgatttt acatggtgtc gacgccgaag aaatgaatct ccgcaatggg gatacgttga
      721 ataaggactg gccaacagat gaaccttaca catttgatgc agtagtaatg aacccacctt
      781 attcggctaa ttggtcagca gatacgactt tcttagatga ttctcggttt aatcgttatg
      841 ggaaattagc gccaaaatcc aaagcagatt ttgcgtttct tcttcatggg ttctatcatt
      901 tgaaggaaac aggtaccatg gccatcgtct taccacacgg agttttattc cgtggcgctg
      961 cagaaggggt catccgtcaa aaattactag aagatggcag tatttatgct gtgatcggta
     1021 tgcctgcgaa cttgttcttt gggacatcga ttccaacgac ggtgattgtg ttgaagaaga
     1081 atcgtcaaac tcgagatgta ttattcattg atgctagtcg tgaatttgtt aaagggaaaa
     1141 atcaaaataa actatcggaa gaaaacattc aaaaaatcct cgaaacctac gctgaaagaa
     1201 aagatgtcga gaaatacgct catttggcaa cctttgatga aatcaaagaa aatgactata
     1261 acttaaatat cccacgctat gtagatactt tcgaagaaga agaaccaatt gatatggttc
     1321 atgtcggaaa tgacatcaag aagatacgac aagaacagca agtgctagaa aaaaaacttt
     1381 tagaagctat ctcttccctg caaacaacac ctgaaaatga agcgtggtta caaggtgcgc
     1441 tggaggtgtt caaacatgag caatgataca caaccagaaa tccgtttccc aggatttaca
     1501 gatgattggg aacagaggaa gttgggggac ttcgttgtag attatgttga aaagacaagt
     1561 gtacaaaatc aatttcctat gctaacttca tctcaacaaa aagggatagt tttgcaagaa
     1621 gactattttg caaatcggca agtaactaca gaaaataata ttggttactt tgtactacct
     1681 agaggatatt tcacgttccg tagtcgaagt gataatgatg tgtttgtgtt taaccgtaat
     1741 gacattattg atagagggat tataagctat ttttatcctg tatttacttt aaaatctgct
     1801 gattccgact tcttcttaag acgtattaac aatggaatcc aacgacaatt atctattcaa
     1861 gcagagggaa caggtcaaca tgttctgtct ctaaaaaagt ttaaaaatat agtagcaatg
     1921 tttcctagtg aggaagaaca acaaaaaatc ggcaccttct tcaaacaact tgacgacacg
     1981 atcgctcttc atcagcgtaa gttagatttg ctgaaggaaa cgaaaaaagg attcttacaa
     2041 aaaatgttcc ctgaaaacgg agcaaaagtg ccagaaatcc ggtttcctgg ttttacggaa
     2101 gattgggaag agcgtaagtt gggagaatta ggtaaaactc aatcgggaat tggatttcct
     2161 gatgccgagc aaggtggttc tgaaggtact ccatttttta aagtttccga catgaataac
     2221 attggtaatg agtatgaaat gagaaatgca aatcattatg tatctaatga gcaaattgag
     2281 cgtaaaaaat ggaagccaat aaaagatgtg ccagcgatta tatttgctaa agttggcgca
     2341 gcaataatgc ttaatagaaa acgtttggtc acaagccctt tcttaataga taacaatacc
     2401 atggcttatt tatttaataa tacttgggat atctattttg gtaaaatatt gtttgaaact
     2461 ataaatttgc caagatactc ccaagtaggg gcattgcctt cttataacag ttctgatatt
     2521 gagaatatca gtgttaaggt tcctgtaaag gatgaacaac aaaaaatcgg tactttcttc
     2581 aaacagctag atgatacgat cgctcttcat caacgtaagt tagatttact aaaagaaacg
     2641 aaaaaaagct tcttacaaaa gatgtttgtc taggagggaa gaagaatgaa aattagtcac
     2701 cgtgaggaag cagaagtcga agaacagttg attcgtgtac ttggagaagg gcataatcag
     2761 tggacgtatc gtccggatct aaaatcagaa gaagaccttt gggtcaattt acgccaaaaa
     2821 attatctcaa ataatcaagc agaactgaat gattttcctt tgaccgataa agaatttgaa
     2881 acgatcaaaa cggaattact gctgcgaacg aaaaccccat ttgatgctgc gaaatggtta
     2941 aaaggtgaaa atgggatggc tcggatcacg attgaacggg aagattcgca attgggttct
     3001 gtctcactta tcctttattc caatcaagac attggtggag gaatctctac ttatgaagtg
     3061 gtccatcaaa ttgcaaaacg tggaagtaac atagaagcac gggatcgacg ttttgacgtc
     3121 accttattaa tcaatggatt accgattgtc caaatcgagc tgaaacaagt aactgcaaaa
     3181 gatggtgttt atcaggcatt taaccaaatc aaaaaatatg cagaagaagg tatgtttcgg
     3241 aacaatatct tttcgacctt acagttgttt gtggtttcca atgaacaaac cactcggtat
     3301 tttgcaaatg ccttaccgaa agacttgcac cctaagtttt tatttagttg gcgaacgaaa
     3361 gacaatgaaa aagtagaaaa cttatatgag ttttgtaaac aagtgttaaa tatcccggat
     3421 gctcatcgtt tgattgcgga ttatacaatc gtcagtgaag atcaagacaa taaaaccctc
     3481 atggtgttac atccttatca agtacatgcg attcaagcct tgtttattgc cgcaaataaa
     3541 catcaatcag ggtatgtgtg gcatgcgact ggttcaggaa agacgttgac tagttttgta
     3601 tctactaagc ttttagcacg aaaatcgggg atcgatcgaa cgatcatgtt agtggaccgc
     3661 aaagatttag ataatcaaac caccacagag tttacaaaat ttgc
//
LOCUS       DAZUMK010000180         3677 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000180 DAZUMK010000000
VERSION     DAZUMK010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.185"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(460..1089)
                     /gene="udk"
                     /locus_tag="ACG808_002757"
     CDS             complement(460..1089)
                     /gene="udk"
                     /locus_tag="ACG808_002757"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFS5048336.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1173..1868)
                     /locus_tag="ACG808_002758"
     CDS             complement(1173..1868)
                     /locus_tag="ACG808_002758"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFS5048337.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1881..2597)
                     /locus_tag="ACG808_002759"
     CDS             complement(1881..2597)
                     /locus_tag="ACG808_002759"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFS5048338.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2927..3532
                     /locus_tag="ACG808_002760"
     CDS             2927..3532
                     /locus_tag="ACG808_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFS5048339.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
BASE COUNT         1048 a          757 c          660 g         1212 t
ORIGIN      
        1 agggggtgcg acaaaagagc ttcttcaaaa aaataagtcg aaatctcaca caaattcgag
       61 aaataatttt cgtaagtttc ttcttattta ttgcatctga ccacttctgt tacaacctct
      121 cgttaaacgg tgttcaaaag cttgcatcta cgataataag cccaacccaa cgaaaaacgt
      181 gaggagctgt tttatttgga tgctcttatc gcttgatgca aagacagctt ttttacaacc
      241 tctacttcac ggtgctcaat cagctgacct tcggtattag ctcaaaaaac ggtaaaatat
      301 aaacagctat tttccgtttc ttctttttaa tacttaggtc aagatgctgt tttcacaacc
      361 tcttgttata aataaaaaaa cagttggctg gtgactgttc aaaaagcaga atccttgctt
      421 ttcaaacggt cgcagtccgt tcaactgttt cgctgcgcac tatttatggt ttaagaaact
      481 agctactttt gttgtaatca aatcaatagc aacgtggttt tctccgcctt ctggaacaat
      541 gatatccgca tagcgcttcg ttggttcgat aaattgatga tacatcggtt taacaactgt
      601 caaatattgt tcgatcacgg agtccaatgt gcgtccccgt tcttccatat cccgtttgat
      661 acgacgaatg atacgaatgt catcatctgt atctacgtat actttgatat ccatcaagtc
      721 acgcagacgt tcgtcttcta aaatcaggat tccttctaga atgatcactt cttttggttc
      781 ctgaatgatc gttgcctgac ttcttgtatg cgctacatag tcatacacgg gtttttcaat
      841 ggctttatag ttcagtaagt cgcctacatg ctgaatcagc agatcattat caaaggcaaa
      901 tgggtgatcg taatttgtgt tcaatcgttc ttcaaaactc aaatggctct gatccttgta
      961 ataagaatct tgttcaagca tcatgatgga atggtccggg aagttgttga aaatcgcacg
     1021 gctgacactt gtctttccgc ttccggatcc acccgtcaca ccaatgataa ttggcttgct
     1081 ttttgtcatt gactaaaacc tctttcgtat ccgttttttc attctaatct attatagcga
     1141 aaaaacacga aaatgctata tttttctgaa aattaatcct tttcaatttc caataatgaa
     1201 taaagttcct ctaatgaatc gattcgataa gtaggctgga tttcttcgat ttctggtaat
     1261 ttatttctat tgaaccaaat cgtatcgatc cctgccatat ttcctccttt gatatcagaa
     1321 gtcaaagaat caccaatgat cacagtttgc tttttatcaa aattaggaat gtgggaaaag
     1381 acatgatcaa aaaattcttt catcggcttt tgcgctccga cagcttctga gacaaagata
     1441 tccttgaagt aatcaagcat tcctgcatct gttaatcgcc gatgttgcgt gtgcgacaca
     1501 ccgttcgtca caatataaag atcagcatgc ggatggacct ttttcaggat ttctagactt
     1561 cctggaacaa gatcatgacc ttggttcaag tagtggcggt agcgtttttc catttgatca
     1621 ctatctactg ttttcccaaa ttgctggaaa agtaacccaa aacgactgcc tattatttct
     1681 tctcgtgata gtctgccttt ttcaaactcc tgccataaat gatgattcaa agccttatag
     1741 ctggtttcga tttctggtgt caatgtgatt ccttgttctg caaacaaagc acttaaagca
     1801 ttggcttcag ttaattgaaa atctaacaat gtatcatcta catcgaacaa taaagtttta
     1861 tatttcatct ttttgacctc ctatttaaaa taaatactta actttttcat ctctgctaat
     1921 gtcaacgatc gatactcgcc tactcctagc tgttcatcta attccaatgg tcccatgctt
     1981 agccgtttca atgcggtcac tttctttcct tgggctaaga acatcttttt gacttgatgg
     2041 aattttcctt cttggatcgt caataaaacc gtactttggt gcttgtctgc tttgagtatt
     2101 tttaaatcag ccggcttaca ttgataccca tcagcgaata cgattccatt ttcaaatgct
     2161 gttacgtctg ccggtgtcac cctttcgttc acaactactt gataagtctt tgaaaccttt
     2221 ttttctggaa gtagcatttc atatcccaat tgtccattcg aagtgatcag caatagtcct
     2281 gttgtatctc tatctagtct tcccactgga tataatggtt ggaaacgatc ggcacttgca
     2341 atcagatcga ttactgtcgt cttttctgga tctgatacag ctgtaacagc tttttcaggt
     2401 ttattgagaa gataatagga ctcatttgtt tctaagtgtt cacctgctac ttcgatttga
     2461 tgcagtaaac tatctacatt tcgattttgt cgaaattcga ctgtgccatc tatcatcact
     2521 tgcttcaaag caaacagccg cttcatttcc ttgcgtgtcg tttctaattt ttcttctatt
     2581 aatttatcta aacgcatgct gtttcctttc ttattactac taattaatta taccttgaat
     2641 attcttgctt gcaaatacat catcatagtt attccttgac ttaattaaca gttcacgtta
     2701 cggttaaata gtaaagtgaa ttggatttct catacacagc gtaatgacct ggtggagaaa
     2761 agttgaatcc tgactttcta aggggagatg cgcgcatctc tccttttgcg cttttttaag
     2821 cgccctgcct tcgatgatgt gcacgagatt agcctttaga attcacttaa ataaaatctg
     2881 tccgttacca aagcagacag tcacatttta aggaggaatt ttggttatgt cagtatcatt
     2941 agaagtaagt aaaagagaag tacgtccccg ttcattaaga aacaaacttc gtcacgaagg
     3001 gaaagttcca gctgttgttt atgggtataa agtagaaagt acccctatct cattcgatgg
     3061 tttgacacta agcaaattgt tgcgtgaaca tggagcgaac acagtcatca cgatggacat
     3121 cgatggacaa aaagtcaata cattggttca caagatccaa acgaacactt ttaccaatca
     3181 tttcgaacat gcagaatttc tatctgtaaa catgtcggaa gaaacagaag tcgaaacaga
     3241 agtcgttctt gttggcgaat cagtgggtgc taaatctggt ggagtgttag ctcaaaactt
     3301 gtatacagta gtcgtatctg ctacaccaga caaattgcct gaacgaattg aagttgacgt
     3361 gacaaacttg gaattaggtg attctattac agtagccgat cttccaaaaa acgacgatta
     3421 caaattcgtg accgacagtg aagagcaaat cgctgctgta gtcgctcaaa gtgaagaaga
     3481 aagcacaggt gaaggtggcg aaccagaagt gatcggttca acagaagaat aatactgaaa
     3541 caatcaggtg gtgttagaag cggcgggtca gcagctttta acacgccttt tttatttcta
     3601 atcgtttatt ttcatatggg gcttccactt ttgtaaatgg ctttacaaac gaaaaatcct
     3661 aatttaccaa gcttttc
//
LOCUS       DAZUMK010000181         3662 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000181 DAZUMK010000000
VERSION     DAZUMK010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.186"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            123..983
                     /locus_tag="ACG808_002761"
     CDS             123..983
                     /locus_tag="ACG808_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-like and DUF2520 domain-containing
                     protein"
                     /protein_id="HFS5048340.1"
                     /translation="MEVIIMNVGFIGAGKVGCSFGKYFQEHKIQVTGFYSKSEDSSLA
                     ASNFTSSKQYLNLRELVDENDTIFITTPDGQIQEVWQEIKNYQIKNKLICHCSGAISS
                     DIFSNIQDYGAYGYSVHPMFSISDKYNSYKKLKKSFITIEGDEKYAKFLCDFFKKLGN
                     STVIVSKENKSLYHAASVVSSNLVLGLINTSVTYLIQCGFTEKMAIEALYPLIEFNIR
                     NIKEKGIIESLTGPVERCDISTIKGHCEVLSDEDRALYMLLSKNVLEIAMLKNINRDY
                     SELEKYLGES"
     gene            995..1822
                     /gene="panB"
                     /locus_tag="ACG808_002762"
     CDS             995..1822
                     /gene="panB"
                     /locus_tag="ACG808_002762"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFS5048341.1"
                     /translation="MKNTAVTFKESKLRNEKLTMLTAYDYSTAKIIDEAGINGILVGD
                     SLGMVCLGHEDTLSVTMEDMIHHTRAVTRGAKNTLVVADMPFMSYQTSVYDSVVNAGR
                     LIKEGRAQVVKLEGGIEVCDKIEAIVKASIPVMAHIGLTPQSVNAFGGFKVQGKDKEA
                     AKELIRAAKAVEKAGAFAVVLECVPTKLAELISKEISIPTIGIGAGAGCDGQILVYQD
                     MLGMYSDFTPKFVKKYANLSEEMNKAFTKYIEEVKDGVFPGPEHGFKISDDVLEKLY"
     gene            1837..2685
                     /gene="panC"
                     /locus_tag="ACG808_002763"
     CDS             1837..2685
                     /gene="panC"
                     /locus_tag="ACG808_002763"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFS5048342.1"
                     /translation="MVIFKTVNDVRSQVKEWKKQGLKVGLVPTMGYLHEGHESLIRKA
                     SKENDKVVVSIFVNPTQFGKNEDLGSYPRDLERDIEVCTRGRATAIFNPEVEEMYCDN
                     ASTFVNITGLTEGLCGASRPIHFRGVCTVVSKLFNIIPADRAYFGEKDAQQLAVIKRM
                     VRDLNIDIDVVGCPIIREEDGLAKSSRNTYLSLEERSSATILNKSLTLAKEALNNGER
                     DSLKIIEIISKNINTYNLAKIDYVEVVDSLSLQRVNYIEKSVLVAIAVFIGKTRLIDN
                     FTFELQ"
     gene            2695..3090
                     /gene="panD"
                     /locus_tag="ACG808_002764"
     CDS             2695..3090
                     /gene="panD"
                     /locus_tag="ACG808_002764"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFS5048343.1"
                     /translation="MKVTMLKGKIHRATVEQAELDYVGSITVDMNLLEAAGIYEYEKV
                     QIVDVNNGNRFETYTIAGERGTGMICLNGAAARCVSTGDKIILMAYCELNENEVKDHR
                     PKVVFVDDNNKVERVTSYEKHGRLSDIIL"
     gene            3292..3662
                     /locus_tag="ACG808_002765"
                     /pseudo
     CDS             3292..3662
                     /locus_tag="ACG808_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1428 a          412 c          724 g         1098 t
ORIGIN      
        1 aatatgggat attatgataa attaactatg gatgatattt gaaaaaaaca tactgtatat
       61 ttgatttaat ttttattttt aagctcatat cgtaagaatg ggacttgtag tctcacttct
      121 ttatggaggt gataattatg aacgttggtt ttattggagc cggcaaggta ggttgttcat
      181 tcgggaagta tttccaggaa cataaaatac aagtaacagg attttatagt aaaagcgaag
      241 actcgtcatt agcagcttcg aattttacat ctagtaaaca atatttaaac ttaagagaat
      301 tagtagatga aaatgatact attttcatta cgactcctga tggtcaaatt caggaggtat
      361 ggcaagaaat taaaaattat caaatcaaaa acaaattaat ctgccattgc agtggtgcaa
      421 tatcttcaga tatcttttca aatatacagg actatggtgc ttatggctat tctgtccatc
      481 ctatgttttc aatatcagat aaatataatt catacaaaaa attaaaaaaa tcttttataa
      541 caattgaagg cgatgaaaaa tatgcaaagt ttttatgtga tttcttcaaa aagttaggta
      601 atagtactgt aattgtatct aaggagaata aatctcttta tcatgccgca tcagtagtat
      661 ctagtaacct tgtgctagga cttattaata caagcgttac atatctaatt caatgtggat
      721 ttactgaaaa aatggcaatt gaagctttat atccattaat tgaatttaat attagaaata
      781 taaaagaaaa aggaattata gaaagtttga cagggccagt tgaacgttgt gatatttcaa
      841 caattaaagg tcactgtgaa gttttatcag atgaagacag agctttatat atgctattat
      901 caaaaaatgt attagagata gctatgttaa aaaatattaa tagagattat agtgaattag
      961 aaaagtattt aggagagagt taggagagaa atagatgaaa aatacagcag ttacatttaa
     1021 agaatctaaa ttaagaaatg aaaaattaac aatgcttaca gcttatgact attcaacagc
     1081 aaaaataatt gatgaagctg ggataaatgg aatattagta ggagattctc ttggtatggt
     1141 atgtctagga catgaagata cactttcagt aacaatggaa gacatgattc atcatactag
     1201 agcagttaca agaggagcta agaacacttt agttgttgca gatatgccat ttatgtctta
     1261 tcaaacttca gtatatgatt cagtagttaa tgcaggaaga ttaataaaag aaggacgagc
     1321 tcaggttgtt aaattagaag gagggataga agtatgcgat aaaatagagg ctatagtaaa
     1381 ggcttctata cctgttatgg cgcatatagg tttaacacct caatctgtta atgcatttgg
     1441 aggatttaag gtacaaggaa aggacaagga agctgctaaa gaattaataa gagctgctaa
     1501 agctgtagaa aaagcaggag cattcgctgt agtgttagaa tgtgttccaa ctaagcttgc
     1561 agagttaatt tctaaagaga tatcaatacc tacaattggt attggagcag gtgcagggtg
     1621 tgatggacaa attcttgtat atcaagatat gttggggatg tatagtgatt ttacccctaa
     1681 atttgtgaag aaatatgcta atttatcaga agagatgaat aaagcattta caaaatatat
     1741 agaggaagta aaagatggag tttttccagg tccagaacat gggtttaaaa taagtgatga
     1801 tgttttagaa aaattatatt aaaagggtca agaaatatgg ttatatttaa aacagtgaat
     1861 gatgttagaa gtcaagtaaa agagtggaaa aaacaaggct taaaggtagg attagttcct
     1921 actatgggat atttacatga aggtcatgaa agtttaatta gaaaagcatc aaaagaaaat
     1981 gataaagttg ttgtaagtat ctttgtaaat ccaactcagt ttgggaaaaa tgaagattta
     2041 ggtagttatc caagagactt agaaagagat atagaagtat gcacaagagg tagagcaaca
     2101 gcaatattta atcctgaagt tgaagagatg tattgtgaca atgcaagtac ttttgttaat
     2161 ataactggat taacagaagg tttatgtgga gctagtaggc caatacattt tagaggggtt
     2221 tgcactgttg taagtaaatt atttaacata ataccagcag atagagctta ttttggagaa
     2281 aaggatgcac aacagttagc tgtaattaaa agaatggtta gggaccttaa tattgatata
     2341 gacgttgtag ggtgtccaat tataagggaa gaggatggac tagctaaaag ttctagaaac
     2401 acatacttaa gtttagagga aagatcttca gctactattt taaataaatc tttaacttta
     2461 gctaaggaag ctttaaataa tggagagaga gacagcttaa aaataattga gataataagt
     2521 aaaaatataa atacttataa tttggcaaag attgattatg tagaagttgt tgattcttta
     2581 tcattacaaa gagtaaatta tattgaaaaa tcagttttag ttgcaatagc agtatttata
     2641 ggaaaaacaa gattaataga taattttaca tttgaattac aatagggggc tataatgaaa
     2701 gttacaatgt taaaaggaaa aatacataga gctacagttg aacaagctga gttagattat
     2761 gtaggaagta ttactgttga tatgaattta ttagaagcag caggaatata tgaatatgaa
     2821 aaagtacaaa tagttgatgt taataatgga aatagatttg aaacttatac aatagctgga
     2881 gagagaggaa cagggatgat ttgcttgaat ggagctgcag ctagatgtgt atcaactgga
     2941 gataaaataa ttttaatggc atattgtgaa ttaaatgaaa atgaggttaa ggatcataga
     3001 cctaaggttg tttttgtcga tgataataat aaagttgaaa gagtaacatc atatgaaaaa
     3061 catggaaggt tatcagatat aattctttag gttttgtaat gttatttata tatagctaga
     3121 ctattattga aatatttatt gattactaat tggatagatg tcaataaagt agacacaaaa
     3181 agagaagata cagactgtag acaaagtcca ttttatgtga atcggctaca ctagactaga
     3241 cagaaaaaat aaggtgtgta gaataataaa aaacacactg gaggattttt catgtcaaga
     3301 cgtcaacgaa gaacctattc aaaagaattc aaacaacaaa tcgtcaatct ctatctcgct
     3361 ggtaagcctc gcgcagaaat tattcgagag tatgagctta cgccttcttc tttcgataaa
     3421 tggatgaagc aagcacaatc aacgggctca ttcaaaaaaa gagacaactt aacaccagaa
     3481 caagcagaat tgatcgcact aagaaagaaa aataagcaac tcgaaatgga gaatgatatt
     3541 ttaaagcaag cggcgctgat attcggacga aaagacaagt aattgatgcc aacaagcata
     3601 aatattccat atcagcgatg tgcaaaattc taaatatttc tcgtcagacc tactattatc
     3661 aa
//
LOCUS       DAZUMK010000182         3601 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000182 DAZUMK010000000
VERSION     DAZUMK010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.187"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..902
                     /locus_tag="ACG808_002766"
     CDS             <1..902
                     /locus_tag="ACG808_002766"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS5048344.1"
                     /translation="SFGQSCNLVNVLSKRDDKVYVGIRSYDWLFQYIFHYYVDKFVYK
                     KADAVVSVSQLIKRDAENIFNFNKDKSYFLYNPYDLELINNKSKEKITEIEVPTDKKI
                     IVTVGRLEDQKGLYHLIKAISMLKNKDNVVVYILGHGTKKELLENLIKAYQLQNVIFL
                     LGGQSNPYKYMSRADLYVMPSITEGFPNALVEAMSTETAVLCADCKSGPREILSNRTL
                     DTICENIEYAESGILVCPMTDSRNYSPQYEECDKNLANAIEELIENDNLRNKYAKAAK
                     KRAELYSYDAFEHNLLQIMKTFN"
     gene            940..2031
                     /locus_tag="ACG808_002767"
     CDS             940..2031
                     /locus_tag="ACG808_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFS5048345.1"
                     /translation="MLKKNTSLQNKTILITGAAGFIGSNLVLELLRTQSSIKIIGIDN
                     MNDYYDVSIKNYRLSEIETEAKNHPGSTWNFIKGSIADKATIDFLFDNYKPSIVVNLA
                     AQAGVRYSITNPDMYIESNIIGFYNILEACRHSYDNGESGVEHLVYASSSSVYGTNKK
                     IPYSTEDKVDNQVSLYAATKKSNESMAHAYSKLYNIPSTGLRFFTVYGPAGRPDMAYF
                     GFTDKLRAGDNIQIFNYGNCKRDFTYIDDIVEGVKRVMQCAPEKIDGEDGLPIPPYHI
                     YNIGNNTPENLLDFVDILQQELIAAGVLPEDYDFESHTELVPMQPGDVPVTYADTSAL
                     ERDFEFKPSTSLRDGLRKFAQWYKSFYEV"
     gene            complement(2099..3577)
                     /locus_tag="ACG808_002768"
     CDS             complement(2099..3577)
                     /locus_tag="ACG808_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFS5048346.1"
                     /translation="MKKLSQKRLAQIIIDSRKSLKMTQQQLAVATGINRALISRVESL
                     DFIPSIQQLESLAEVLNFEPTDLFINESNKSNRTKHQSKNIAVAGTGYVGLSIATLLA
                     QHNQVTAVDILPEKVKQINNKKSPIQDDYIEKYLAEKNLNLIATTDGAAAYREADFVV
                     IAAPTNYDSKQNYFDTSAVESVIKLVLEVNPKAIMVIKSTVPVGYTESIRKKYNTKNI
                     LFSPEFLRESKALYDNLYPSRIIVGTDQTDKTLREASHTFADLLQEGAIKKDIETLFM
                     GFTEAEAVKLFANTYLALRVSYFNELDTYAEMKGLNTQEIIHGVCLDPRIGTHYNNPS
                     FGYGGYCLPKDTKQLLTNYNDVPQNMISAIVDSNRTRKDFIADRVLELAGTYEANSSW
                     NAEKEKEVVIGVYRLTMKSNSDNFRQSSIQGVMKRIKAKGATIVIYEPTLENNTTFFG
                     SKVINNLDDFKNISHSIIANRYDSNLDDVKEKVYTRDLFQRD"
BASE COUNT         1180 a          546 c          650 g         1225 t
ORIGIN      
        1 tatcttttgg acaaagttgt aatttagtaa atgttttatc aaagagagat gataaagtat
       61 atgttggaat ccggtcctat gactggctat ttcaatatat attccattac tatgtagata
      121 aatttgtata taagaaagca gatgctgttg tgtctgtcag tcagttaatt aaacgtgatg
      181 ctgaaaatat tttcaatttt aataaggata aaagctattt tttatataat ccatatgatt
      241 tagagttgat aaataataag agtaaagaaa aaattacaga aatagaggtt ccaacagaca
      301 aaaaaattat agtgacggtt ggtcgattag aagatcaaaa aggtttatat catctaatta
      361 aagctatatc tatgttaaag aacaaagata atgttgttgt gtacatctta ggacatggga
      421 caaaaaaaga attattagaa aatttaataa aagcatatca actacaaaac gtaatatttt
      481 tgctaggtgg ccaatcaaat ccttataaat acatgtcacg tgcagattta tatgtcatgc
      541 catctataac tgaaggtttt cctaatgcat tagttgaagc aatgtctaca gaaactgctg
      601 tcttatgtgc agattgcaaa tctgggccaa gagaaatatt atcgaataga acgctagaca
      661 cgatttgtga aaatatagaa tatgctgaat caggcatatt agtatgtcca atgacagaca
      721 gtagaaatta ttcgccccaa tatgaagaat gtgataaaaa tttagcgaat gctatagaag
      781 aattgattga aaacgataat ttgaggaata agtatgcaaa agcagcaaaa aagagagcag
      841 aactatatag ttatgatgcc tttgaacata acttgttgca aattatgaag acatttaatt
      901 aattcatatg atgaaataaa aataagggag tataactaga tgctgaaaaa aaatacatct
      961 ttacagaata aaacaatatt aataactggc gcagccggtt ttattggatc aaatttggtt
     1021 cttgagttat tacgtactca gtcatcaata aaaatcattg gcattgacaa tatgaatgat
     1081 tactatgatg tctctattaa gaactataga ttatcagaga tagaaacgga ggcaaagaac
     1141 catccaggat ctacttggaa ttttatcaaa ggttctattg ctgataaagc aacaattgat
     1201 ttcctctttg ataattataa accaagtatc gtggtgaatt tagcagctca ggcaggagtc
     1261 cgttattcaa ttactaatcc agatatgtat atcgaatcta acataatagg attctataat
     1321 attcttgaag cttgtcgtca ttcatatgat aatggagaat ctggtgtgga acatttagtg
     1381 tatgcctcat cgtcttccgt ttatggtact aataagaaaa ttccttattc tacagaggat
     1441 aaagtagaca atcaagtatc gttgtatgca gcaactaaga aaagtaatga atcaatggca
     1501 catgcatatt ctaaacttta caatattccg tctacagggc tacgtttctt tacagtatat
     1561 ggtcctgctg gtcgtccaga tatggcttat tttggtttta ctgataaact tcgtgctggt
     1621 gataatatcc aaatattcaa ttatgggaat tgcaagcgtg actttaccta tatagatgat
     1681 attgttgaag gagtaaagag ggttatgcag tgcgctccag agaaaattga tggggaagat
     1741 gggctaccaa tcccaccata tcatatttat aatattggta acaatacgcc ggaaaaccta
     1801 ctagattttg tagatattct acaacaagag ctaattgcag caggggtgct accagaagat
     1861 tacgactttg aatctcatac cgaactagtt ccaatgcaac caggagatgt gccagttact
     1921 tatgcagata ctagtgcgtt agaacgagat tttgaattta aaccaagtac aagtttgcgt
     1981 gacgggcttc gtaaatttgc gcagtggtat aagtcttttt atgaagtata attgtatttt
     2041 tttacttata aaagtttata gaaacacctc agacactaca aatctgaggt gttatttttt
     2101 aatctcgttg aaataaatct ctggtatata ctttttcttt tacatcatca agattactat
     2161 cgtaacgatt agcaataata ctgtgactga tatttttaaa atcatctaaa ttatttatta
     2221 cttttgaacc aaaaaatgta gtgttatttt caagtgttgg ttcataaatt acaatagttg
     2281 ccccctttgc tttaattcgc ttcataactc cttgaataga tgactgacgg aagttgtctg
     2341 aattactctt cattgtaaga cggtatacac ctatgactac ctctttttct ttttctgcat
     2401 tccacgaact gttagcctca tacgtgccag caagttctaa cactcgatct gctataaagt
     2461 cttttcttgt acgattacta tcgacaattg cacttatcat attctgagga acatcgttat
     2521 agttagtgag aagttgtttt gtatctttcg gtaaacaata gcctccatat ccaaaagatg
     2581 gattattata atgtgtgcca attcttggat ctaagcaaac cccgtgaatg atttcttgtg
     2641 tatttagtcc tttcatttct gcataagtat ccagctcatt gaagtaagat actcgaagtg
     2701 caaggtaagt attagcgaat aatttaactg cttcagcttc tgtgaatccc atgaaaagag
     2761 tttcgatatc ttttttgatt gctccttctt ggagtaaatc tgcaaaagtg tgagaggctt
     2821 ctcgtaaagt tttgtcagtt tggtccgtgc cgacaataat tcgcgaagga tatagattgt
     2881 catataatgc cttagattct ctgagaaatt ctgggctgaa tagaatattt ttagtattgt
     2941 attttttacg aatactttcg gtatagccta cgggtaccgt cgacttgatg accattattg
     3001 cctttggatt gacttctaac accaatttga ttactgactc tacagcagaa gtatcaaaat
     3061 aattttgttt actatcataa tttgttggag cagcaatgac aacaaaatcg gcttctctat
     3121 aggcggctgc accatcagtc gttgcaatta aattaagatt tttttcagct agatattttt
     3181 caatgtaatc gtcttgtatt ggagatttct tattgttaat ttgttttact ttttcaggaa
     3241 gaatgtctac tgctgttacc tgattgtgct gggcaagaag agtagcaatg gaaagaccta
     3301 cgtaaccagt accggctaca gcaatattct tcgactgatg ttttgttctg tttgatttat
     3361 ttgattcatt tataaataga tctgttggtt caaaatttag aacttctgct aatgattcta
     3421 attgttgtat tgacgggata aaatcgagag attctactcg actaattaag gcacggttga
     3481 taccagtagc tactgcaagt tgttgttgtg tcattttcag agattttcta gaatcaataa
     3541 tgatttgagc taaacgtttt tgagataact ttttcatgat gcactccttt ttttgttgct
     3601 a
//
LOCUS       DAZUMK010000183         3451 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000183 DAZUMK010000000
VERSION     DAZUMK010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.190"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(25..360)
                     /locus_tag="ACG808_002769"
     CDS             complement(25..360)
                     /locus_tag="ACG808_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS5048347.1"
                     /translation="MGVEAVDKYLYLLAGNKIQKSLMDFIQELECTFHKKFTHSILLK
                     LLIHTACLIERTLINGHELKIISEDDTRPSHETIFHVKKAFKNIETEFGITVSYDECF
                     FIYDIIASK"
     gene            646..2526
                     /locus_tag="ACG808_002770"
     CDS             646..2526
                     /locus_tag="ACG808_002770"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS5048348.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIIIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDNVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDQFSQYYTPTVLLIALLVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQRGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAMLKQAGAKHLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLH
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSSFGSLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLIGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            2605..2832
                     /locus_tag="ACG808_002771"
     CDS             2605..2832
                     /locus_tag="ACG808_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFS5048349.1"
                     /translation="MKKMIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSTKVKEA"
     gene            2837..3379
                     /locus_tag="ACG808_002772"
     CDS             2837..3379
                     /locus_tag="ACG808_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFS5048350.1"
                     /translation="MNIVEEKFAQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLKQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
BASE COUNT         1140 a          571 c          724 g         1016 t
ORIGIN      
        1 aatactcgtt tacataaaaa aagattattt agatgcgata atatcataga taaagaaaca
       61 ttcatcataa gaaacagtga tcccaaattc tgtttcaata tttttgaagg ccttttttac
      121 atgaaaaatt gtttcatgag aaggtcttgt gtcatcttct gatataattt taagttcatg
      181 accgtttatc aatgtacgct caattagaca cgctgtatga atcaaaagtt ttaaaagaat
      241 ggaatgagtg aattttttgt ggaaagtaca ttccaattct tggataaaat ccattaagct
      301 tttttggatc ttattcccag ctaataagta gagatatttg tccactgctt caactcccat
      361 atcaaaaaac gatatttctt gtgaatgagt ggctgctgcc cttttttgat tgttgttctt
      421 cagtcgtaag atgacaaagc atcttatctt tgaataaaaa aatagaacta cctaaggaag
      481 cgtttcggcg cttccttttt ctatatgatc gtttaatgac tgtaggaatt cagtatacca
      541 aaagaaaacc gcctttttga ataaacttga tagtggtcaa ttttttatga tggataagta
      601 tctatactta gtctcgtaag aatgaaaagg aaaagaggga gaaagatgaa attacaaaag
      661 tggatccaag agcatcgaaa tcatatcacc gccttatcag ctgtgttgat tatttttggt
      721 tttatcggga aatttactgg atttgtcttg ttctataaca ttgctctagc catagcttct
      781 attattgcgg tcatacctat tataatccat gcgtatcagg cagtaaaagt caaagttatc
      841 tcaattgatt tactcgttac tattgctgtg cttggtgcct ttatgatcgg agaatacaag
      901 gagtcagcta tcgttacatt cttgtttttg tttggtagtt atctggaaca aaaaacattg
      961 accaaaacgc gggaatcaat caaggctttg accgagatgg ccccatcgac tgccagcgtt
     1021 gtccaagaag atggaacgac cgaagaagtg gaaatcgatg acgtggaaga aggcgatatc
     1081 gtcttagtaa aaactggtgg agctattcct gtggacggac gagtcattga cgggaaaggg
     1141 tatgcggatg aagccgcagt gacgggtgaa tcacgacaag tcgtcaaaat cagtggagat
     1201 aacgtatttt ctggaacgat tttagcagat ggctacgtga cgatcgaagc aactaaggtc
     1261 ggagatgata cgacctttgc caaaattata gaattagtcg aagaagcaca agataccaaa
     1321 tcacatgcgg aaaaattcat tgatcaattt tctcagtatt atactccaac cgttttactt
     1381 attgcgttgc ttgttggtct gatcagtcgt gattttcgtt tagccatcac tgttttggtt
     1441 cttggttgtc ctggcgcttt agtcattggt gcgcctgttt cgaatgtagc aggaatcggt
     1501 aatggagcga aacggggcat cttagtcaaa ggcggtgaag caatggatac tttttccaaa
     1561 atcgatactt ttgtctttga taaaaccggt acattgacca aaggaactac tgctgtatcc
     1621 actatcaaga actatacaga ggatgaaggg gacagcttag ctttagtggc tcgattagaa
     1681 acgttatccg atcatcctct ggggcgcgcc gtgatccatt atgctcagga gaaagacatt
     1741 gattttcaac aattagcagt caaagataat cagacgatca agggacaagg aatcacagct
     1801 gagattgatg gtcatacagt ttgtgcgggc aacaccaaat tgattgccgc tcacaaactt
     1861 tctctcacac caaagcaagt acaagattta catcaattac aacaaagagg cagctcagtc
     1921 attattgtag caatcgacaa aaaaatcact caaatcattg gtgtgtcaga tgtgattcga
     1981 ccagaagtcg ctgaacaatt agctatgttg aaacaagcag gagcaaaaca tttagtgatg
     2041 ctgacaggtg ataatcagat gactgctgat tatgtagctg acatgttagg cattgatgaa
     2101 gtccatgctg aattattgcc agatgaaaag gttcaatttg tgaaaaaata ccaagaacaa
     2161 ggattgcacg ttgctttcat tggagatgga atcaatgatt ctccctcttt ggctgcggct
     2221 gatatcggga ttgctatggg atcaggaacg gatgtcgcta tcgaaacgag tgacgttgtc
     2281 ttgatgcaat ctagttttgg atcgttggtt catgcttacc gtttggctaa gaaaactgtt
     2341 ttgaataccc gtgaaaatgt agtgattgcc attggtgtcg tcttattttt actcatcggc
     2401 ttatttgccg ggtttattta catggctagt gggatgttcg ttcacgaagc tagtatcttg
     2461 atcgttatct ttaacgcgat gcgtttattg tattttggac acgaaagcaa agaagaaaaa
     2521 tcgtaaaact tgataagaat caattttttt atggaagctg taatctagaa taagactatc
     2581 aagaaaaagg aaggaagaac tacgatgaaa aaaatgatta tgcaattaga tacactagct
     2641 tgtccaagct gtatgcaaaa aatagaacat gcggtttctc aacagcctgg ggtaagcaat
     2701 acgaaagttt tgtttaatgc gagcaaaatc aaagcaaact ttgatccaga tgaagtccac
     2761 cctgaacaat tagctgcagt tgtggaaaaa ttaggttatg aagtccaatc aacaaaagta
     2821 aaagaggcgt aaatcagtga atatagtgga agaaaaattt gctcaagaaa caaaacaagc
     2881 agatatcgat catcataaac caactgctgg cgccatggtg gggcatgtat tggcaaattt
     2941 taaattagaa gaaatgaaat tgatccaagc acaattgtat gttttgggta catcttcgca
     3001 agaattaagt accctttttt cagaatatgc tgatcaagaa gcgagatggt tcaataaaat
     3061 ctctcatgag ttagtgattg aaaatgaaat cgttccgtct actttagaag aagtcatgcg
     3121 ttatggaaaa atttacgaac atggtcaaaa caaatattta ccagcctcaa caatgttgga
     3181 aaactttgca aaagattttg atttccaaaa tgtctttatc acacgggcaa tcaaattagc
     3241 tgaaaaagaa gaaaaatatg gcttgaaaca attgcttgtt gaacttttaa gtttcaataa
     3301 agaaatcatc taccaagtac aaagactcca aggaaaaact gtccgacaag atttggatga
     3361 ggaagacgat gatgactaaa agtctatgtt tcttcaaata caaatataaa gcgtgaggta
     3421 aattggaaaa tcgcgaagat taaaataaaa g
//
LOCUS       DAZUMK010000184         3415 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000184 DAZUMK010000000
VERSION     DAZUMK010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.191"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(560..3265)
                     /locus_tag="ACG808_002773"
     CDS             complement(560..3265)
                     /locus_tag="ACG808_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFS5048351.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLQIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
BASE COUNT          905 a          682 c          623 g         1205 t
ORIGIN      
        1 tatagtcgaa aatttatagt aaacaagatg ttatagtacg ctcactgacc ttaaaagtca
       61 ataaacttta agaaaataat tggaaaaaaa cagaaaaata tcaagcgctt tttcaaacgc
      121 tttaatacag caaaaatcta acattttttt atgggctttt tctttgaatt ctataataaa
      181 aggatagaac ttgacttaga aaatcaagtt ctatcctttt tgatatctta acttttggaa
      241 gagagatgta aggctgcttt ctacccatca ttcttactac tcgaagcata gcgcagttgt
      301 cacaacctct tgataaacgg tgtttaaaag cttgcatctg cggtaataag ctcaaccaaa
      361 ttaaaaacat aagaagctgt ttttatttgg tcgttcttat tacttgatgc agagcagctt
      421 ttttacaacc tcttggtaca cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa
      481 acggcaaaat atgaggaact atttttccat ttttttctct aatactcagg tcaaaacact
      541 gatttcacaa cctctttttt taatgaaaaa tatggctctc atgcgccgct tctttgaatt
      601 tcgtcatttt ttcttcgatg aacggataga ttgccgttcc tacgattcca aagacaacaa
      661 acaaaccgat catgatccag attgctttcg tagcatttgg ccaataaatt cctcctgcag
      721 attcccgtaa caagtttacc gcatgagtaa atggcaagaa cggattgatc atttggaaga
      781 atttccctga gacttggatc ggatagttcc cgcctccgcc agagatcgac agtactaaga
      841 tgataatagc gatcccttta ccgacattat cgaataatgc agccaagaca tagacgatca
      901 tcatgaacgc taaggcgatc aagacagcaa atgacacgct gtagaccgga ttttggacat
      961 ctactcctag taagaaaata ttccctaatg taacgatcaa gctttgcgca atcgccattg
     1021 tcaaaaatgt cagcatacga gcaacgaatt gttctcgttt cgtaaaattg tttcggtctt
     1081 tctttcctaa atagtattct gtcgtagcaa cacttgaaag aagaacagcg ccgacccaca
     1141 gacatagtgc agtataaaac ggggtgctag ctgaaccgtt atttgcgatt ggataaagcg
     1201 cattggtttg tagctcaaca ggttgtgtaa agaagtcact ttcttttgtt gcgtctagtt
     1261 tcaataattt gatgacttcg cctagatcga cttcttgttc gccttttcgg atcgcgtctg
     1321 ctgctttttg gataccagct ttgatatttg gccagtcatt gttgatcaag tccgcagcag
     1381 cgttcacagc tgtttcgact tctggcattt tttcatttac tgttgtcaat gtaccggtga
     1441 tttcattttt tagtgctggc caatcatttt ggataaaatc agcggccaat cctaattttt
     1501 gctgaatgat cggtaattca ttgttgtata ggtctgcccc ttcattgatg ccattgacga
     1561 tggtatccat atttccattc agcattgtat tggcatcatg gatttcttgt ccaattgcag
     1621 gcatttcttt ttgatatttt tctaaaattg ccactgcatt cgttacagtt gctttcgttg
     1681 agctgagaag cgaatcaaag tcgatctggt ccgctttgct cagtgcgcct tgtgcagtcc
     1741 caatcgtatc gatgacttgg tctagtagag aagtgacagt ggaactgatt tgacctgtat
     1801 tgataccagc aatgatgcca ttgatattat taacaacggc atcaatttga tctagcaatt
     1861 catcaatagc cttcgtgtct tcattttgaa cagctgtgtt caaagcagat aggcggtttt
     1921 gcagattgct tagcaagggt tttaattgat ttagctgatc aataattggc tgtaatttcg
     1981 tatttccagc acttgtttgc aatttagtca ggaactcgac cagtttatcg attgcatttt
     2041 gttgttgaga tattccgtca ataaagccgt tcaatacatt gctgatgttt ttcttttcat
     2101 catcagtcag cttaccatct tcaatagctg ttcttacagt atctattaac gaacctgtcg
     2161 ttgttgccac ttgtccaatt gacttcaatg taacatctac actgtttgtg atgcttggta
     2221 tcgcatcttt cagctgagag gcacctgatt ttgtttggtt tgctagatca cttgcctgat
     2281 tttctaaatt tttcacatct ggcaagatat cttgtacttg ttggatgatc gtcaagcctt
     2341 gttttgcttc atcgattcct tggttcattg tctcttggat cgaagcaaaa tcgccgtcga
     2401 tctctgctag ttgcttaccg gcattttgga tctctgggat tttttcttgc aaatcaagga
     2461 tgatttttgc ttgatctttc aactcaggca ttttgtcatt caatgcaacg actttttcac
     2521 ccatctcatc tactttaggg atgtaatcca cgaattcatt tgctttattc agtttttcct
     2581 tgatttcagg tagttgactt tgtaattcga ggacttgttt tgtgtagccg tcgatcgtgt
     2641 ccaaattttc atctgttgat aaaatcatat ctttaacttt attgatactt actaggttcg
     2701 tatcgatgtc gtaacctatc tcattgaata ctttcaataa agtcgaactg gctgttttga
     2761 taaattcatt tgtgatttgc gattggatcg aacttgctcc tttatcggtg attttcggtg
     2821 caatcgcatt gattttttca tttaccgtat attcgatttt tggtttcttg atatcaccac
     2881 tagtaaaact cagcagatct tctgagaaat cctttggcaa gtaaatacct gcgtaatact
     2941 ttccggaacg gactccgtct tctaattgtt cttttgaatc aacgaattgc catcctaatt
     3001 gtttattttt atgaagcgat tcgatgactt gctccccaat ggccacttct ttaccttgaa
     3061 actctgctgg cttgtctgca ctgtaaacag caattggcag ctctccagta tttccatatg
     3121 gatcccataa tgctttgatg ttaaaccacg catatagcga agggatgatc atgatcgcga
     3181 tgatctgaag tgttgcaatc gggtttttga aaatccgttt ccagtcaagc aaatataatc
     3241 gaagcgtatt tttgattgac tgcatagttc tcccccttct tcttttttca gctgaattac
     3301 tttaccatgt atttcatact tttttcaatc ggcttttttc aaaaaagtcg aatttttgga
     3361 catatcacgg caattgtctc attgtaatct cttacatatt gagaaataat taagg
//
LOCUS       DAZUMK010000185         3389 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000185 DAZUMK010000000
VERSION     DAZUMK010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.192"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..113
                     /locus_tag="ACG808_002774"
     CDS             <1..113
                     /locus_tag="ACG808_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS5048352.1"
                     /translation="SVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            607..894
                     /locus_tag="ACG808_002775"
     CDS             607..894
                     /locus_tag="ACG808_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048353.1"
                     /translation="MEIKEIKVRGINKKYVQEIDHRCVELTEQTGQKWSRNDYLKLLI
                     ENDFERPLMDYKKDQFDRLLEKFTDVQLHNTKVLEAYTNEVKNLIEILIAH"
     gene            912..1334
                     /locus_tag="ACG808_002776"
     CDS             912..1334
                     /locus_tag="ACG808_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048354.1"
                     /translation="MVKAIEKHITFYEAHDEVLKHIMETQPTVKNYSEAIRFLCMNYD
                     PEIEKNNNMTNAKLNAMGKEISILIEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKSINESVHARVDPFDPTKFLKR"
     gene            1354..2946
                     /gene="mobP2"
                     /locus_tag="ACG808_002777"
     CDS             1354..2946
                     /gene="mobP2"
                     /locus_tag="ACG808_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFS5048355.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMGNGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWHYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQNSQIKPLIESMD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQIEQERQ"
     gene            3202..>3389
                     /locus_tag="ACG808_002778"
     CDS             3202..>3389
                     /locus_tag="ACG808_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048356.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT         1357 a          522 c          644 g          866 t
ORIGIN      
        1 ccagtgtcag caatcaacgt aatcatattc caagaaaatc attgaattac agaacaccaa
       61 tcgaaatatt tttgagctat gtacaagaag cattttattc tagcttaatt tgacaaatca
      121 tatgaaaaaa gtatataaaa aatgtttatg gatcagctat atcaccctag gtatagctgt
      181 taaagagatg ggagaaaaca ttaaccacga ttctaacaca aacatcaagc acataaaaca
      241 tttaacactc ctcctttctg gctgaagatt tcaaccaaac aggaagacag cgtatcaatt
      301 atagcataca ctttttctta aattcatttt tcatatgaca aaatatttcc ttctaatcga
      361 caaagttaga aggatttttt attgtctaaa tacacaaata accttaattt ttaaagagca
      421 taaggaaacg tgggaacact ttttaaaaaa agtgttccaa taggctgtgc ctattacgtt
      481 tctctaaaac aaaagtgggt acaaaatatt cgatttatgg atagtgtatg gatagtaaaa
      541 cagctataca tggatagcat atggatagac tccatagact atccatactt ttttcggagg
      601 gtgaaaatgg aaattaaaga gattaaagta agaggtatta ataaaaaata tgttcaagaa
      661 attgatcata gatgcgtaga attaacagaa caaactggtc aaaaatggag tcgaaatgat
      721 tatctaaaac tattgattga aaacgacttt gaacgtcctt taatggacta caagaaagat
      781 caatttgatc gtttattgga aaaatttaca gatgttcagc tgcataatac aaaagttttg
      841 gaagcataca cgaacgaggt caagaactta attgaaatat taatcgcaca ttaggaagga
      901 gataatgttc tatggttaaa gcgatagaaa agcatattac attttatgaa gcgcacgatg
      961 aagttttaaa acatattatg gaaactcagc ctacggtaaa aaactattct gaagccattc
     1021 gatttttatg tatgaattac gatccagaaa ttgaaaaaaa taataatatg acgaacgcaa
     1081 agttgaacgc aatgggaaag gaaatttcta ttttaataga aatggtttca gagtttgcgg
     1141 atcttcattt gaaagataca ggaatattaa ttggtacaaa ttcaactgtc tatcaagaag
     1201 caaaagagcg tgtggaaggg aaaataaaag cgaatcaaac gaagaagtat tctcctaaaa
     1261 tcaagaaatc aataaatgaa tctgttcatg ccagagtaga cccgtttgat ccaacaaagt
     1321 ttttaaaaag ataaaaagta gaaggggaat atgatgaata aatcgcctgc agtcattctc
     1381 acttgtcaat ttaccttgcc caataaaaaa tctttttcaa catacattga ttatatgaca
     1441 agagaaaaag cgctggaaga aatggaacga cgtacgcctg aagaagaaca ggaactgcaa
     1501 cgaatcaaga atgctttaga ggatttttat attcctgaag gagaaacttt ttcggagagt
     1561 aagacggata aagaattcag cgaaacgtcg aatgaagctc aatctttgat gggaaatgga
     1621 atcaactttg acaagttaga ggaacaagat tttaccaaat atctttctta tatgacccgt
     1681 cactatgcgt tagaacaaaa gaaagaactg acgcaaagtg aacaaaaaga ataccaacgc
     1741 ttgaaccaag caataaaaaa atatcaaaca gagccacaca aaaaagcttc aggaaacctt
     1801 cctggagttt tttctatggc ttcagacgaa gtgaaaggaa gtgatctgaa ggagattaag
     1861 caaatcttcc aaagaggaca acaaaaaggc tcaatcattt atcaagatgt tgtctctcat
     1921 gataattcgt atcttgaaaa aataggattg tacgatccca aaacagatac tttagatgaa
     1981 gaaggattaa aagcagctgg aagaaaaatg atggagacat tattcgagaa agagcaactg
     2041 aacgaaacag gctactggat ggcaacgatt caccgtaata ccaaacatat ccatattcat
     2101 tttgcagttg ttgaaaaaga aaatacgcgt aaaccttaca cggaggttga aaatggtgtg
     2161 agttatatcg taccgaaagg taagagaaga caagcaacat tagacgcaat gaagaccaat
     2221 tatgtacatg agttagaacg tttttcttat gaaaaggatc gaatgattgg tatggagaaa
     2281 cgaaatttat tgacgcggaa aagtgatttg cgtaacacct taacaaaagt ggtaaaagag
     2341 ccagcgcgtt acgatcgaca cgcaatgaac ttattaaatg aagtatataa aaccctacct
     2401 gacaaacgtt cggaatggca ttatggggat gaaaaacgaa ccaaactttc accatacaca
     2461 agagagaaac tcgatgagtt aaccaaatat gttttaacaa aagaacctga atatcaagaa
     2521 tatatacaga tttcaaaagc attgaaagaa gaagcaaaaa gtctttatgg ggaaagcaaa
     2581 agagaatcaa aggatgcaga aaaaaatgct ctctttgatt tgaaaaagcg cacaggaaat
     2641 gccattctaa cagaactgaa gaaacagaat agtcaaataa aacctttaat agaaagtatg
     2701 gatcagatgc aatctattta caatgaacga tcctttcgaa cgcttaaaca gttaccaacg
     2761 aatgaataca aatatagtta cgaagaattc atgaagcgca ggaaacaatt ccaacaaaga
     2821 gttgtcacgc aacggagttt gcgtcaatta aatcgaacga ttaatgccca tgaagaaaaa
     2881 taccacgcaa gtaaagccta cgaagagcaa caacgaagaa tccaaataga acaagaaaga
     2941 cagtaaagac agtaaagtta gcgccttatt atactacttg ggaaatgaat gaagagggga
     3001 tcaataactt cccgtgtaaa agcattgaag agtctatttc actcacagaa tgttagttgt
     3061 taagtgaata aaagaagtaa aagagtcctg aacgtaattg tccaggactc tttttttcaa
     3121 tgaattgttt tccgccaatt caatgagtgg tgcgatttgc gactacatta tatcaaaaga
     3181 aagagaaggg aggaagaaga aatggcttat tcaacgattg gagttgttgt tgaaaaaagc
     3241 agagacaatc tcgtttttgt gacagaaatt caaacaggca gagcttttgt cgtcacagac
     3301 aaagcggcaa aagcgtatca aaatggcgat attttaacgt taaatctgac tacgaagacg
     3361 ttcgtcgatg cggcagaaga ttatccatt
//
LOCUS       DAZUMK010000186         3355 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000186 DAZUMK010000000
VERSION     DAZUMK010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.193"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..753
                     /locus_tag="ACG808_002779"
     CDS             <1..753
                     /locus_tag="ACG808_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS5048357.1"
                     /translation="DFILNSGKTIIYSTMSDIKKNTKVGDATIIRFCQKIGFSGFSDL
                     KIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYSTIRLLDEEKLNEAIKMITQSKNI
                     YIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQAQVASLLTVNDLVIIFSLSGKTK
                     DTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQTAIEEFLNGGSLAGKISQLYIC
                     DLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            983..1213
                     /locus_tag="ACG808_002780"
     CDS             983..1213
                     /locus_tag="ACG808_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFS5048358.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(1436..1534)
                     /locus_tag="ACG808_002781"
                     /pseudo
     CDS             complement(1436..1534)
                     /locus_tag="ACG808_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(1799..2254)
                     /gene="tnpA"
                     /locus_tag="ACG808_002782"
     CDS             complement(1799..2254)
                     /gene="tnpA"
                     /locus_tag="ACG808_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFS5048359.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            2497..3355
                     /locus_tag="ACG808_002783"
                     /pseudo
     CDS             2497..3355
                     /locus_tag="ACG808_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
BASE COUNT         1107 a          579 c          585 g         1084 t
ORIGIN      
        1 gattttattt taaattctgg gaaaactatt atttatagta cgatgagtga tattaagaaa
       61 aacacaaaag ttggcgatgc tactattatt cgcttttgtc aaaaaattgg tttttctggt
      121 ttttctgatt taaaaattga aattgcaaag gaagacttta gtcaaaaaaa agaacatccg
      181 actagtgtaa agtactatga tgaaattgct aatagtttaa cagaagctct ctattccact
      241 attaggcttt tagacgaaga aaaattgaat gaagccatta aaatgatcac tcaatcaaaa
      301 aacatatata tatttggagt gggatctagt ggaaatacta gtcttgattt agaaaatatg
      361 tttcttcgcg taggagttca agcaaaagct gtactagacc ctcattttca agcccaagtc
      421 gcttctttat taacagttaa tgatttagta atcatttttt ctttatctgg gaaaacgaaa
      481 gatacttatg attcattgaa aatagctaaa aaaaatggcg caaaaattat tgctataact
      541 aactatattc attcacctat aggaaagtct gcagacttag ttttacaaac agccattgaa
      601 gaatttctta atggtggatc tttagctgga aaaatatcac aactttatat ttgtgatcta
      661 ttagttcatg gttatgaaca aaacaataat attgattcag ttgaattaag agaaaaagtt
      721 ttgcgctcta ttattgataa aagtattgag tgaactaccc ccacttagct aaacctaacg
      781 gtttttaacg attgaagtag gcaattcttg ggatgagagc atacttgata gcttattttt
      841 tactaatagc ggtggctctt attgaccaaa tatccctgta gttcctacgg ttcttgattt
      901 tatctaaaga tatttttatc gtggaagcta ttgatcaact tgaaagaaaa acgtgtagct
      961 cattatcact agtttatcga gtatggatga agcaatggat catcctctct taaataaatt
     1021 tattaaggat ctgatccttc aaattttttc catgatcgat gagcaggaac ctactgaatc
     1081 taaacgacat cgggcacaag gaattattaa gattgctaag cgaatgtttt ctataaaggg
     1141 tgttctaagc tatacagtgt taattcaaaa gattcccaat catcttgttt ataagaacaa
     1201 cgttgaagat taaaaaagtg tttcaatgct aaaattgcaa aatacttcaa tgttatgaga
     1261 caaactgtct ataagaatca atttccaccc atttcatcac ttgttaaaag tttatgatcc
     1321 ttttgatttt tatattaatt tatctgtatt taatacagat tttatttatt agttcatctg
     1381 cctattgtat tttattttgt tgccttacca tctgaagtaa atatactctt aattgtcaaa
     1441 ttaagttaga cagatcatca atacttacgt aactcaaaaa tacttccaaa ggtgttcgat
     1501 aatccagtga ttttctagga agattatttc tgcgccagca ttttctaatg tttgaatttg
     1561 tgttttgtaa tcttggttgc ttgtgctaat tcttgaatag ccgtaaattg ccaaaatttc
     1621 atcacccttt ctggacataa gtttctgaca ccagttaaaa tcaagtctat cagtaaccca
     1681 tacacccgtg aggacgggtg tatttttaat ccccctagaa ggggtcgaca ctcgctgccc
     1741 cttaaaaggg gatttttaaa agaccgccaa ctgctcatct tcactcactt acttctcttt
     1801 attttttaaa tggttctacg tactctcttt tacttataat atatcgaatg cgatcatcag
     1861 cttcttgttc acggatgtat ttttcatttg tcttttgatt caatccgact gtactcacat
     1921 agtatctctt cgaccaaaag gatttgtttc cgtatttata tttgagattt gcgtgctgct
     1981 cgtggattag taccgcacta cgacctttca gataccccat aaaactggat atacatattt
     2041 ttggtggtac tcgaacaagc atgtgaatat gatctagcat tgcatgtgct tctatgattt
     2101 ccacatcttt catttcgcac aacttcctaa gtatttttcc aagatctttt cgtaattgcc
     2161 catagattac ttttcgttga tattttggtg taaaaataat atgatactta caattccaac
     2221 gagtatatgc taaactttta tcgtcatttg acatgaaaaa actcctttcg atctgatgag
     2281 cggttggtag ccactcacta gtatatcgaa aggagttttt atcgctaaag atacttcttc
     2341 cacgcgtaga aacgcgcggt tttcttgttt cgcacgcttt acgcacaaaa ctgactgaag
     2401 tcattaagaa aataaaaggg tctaaaactt ttaagttttg taccgacccc caaaagttag
     2461 accaaaaaat ctaacttttg gaggtcggta ttttttatgg ctaagtatac ttttgaactt
     2521 aaattaaaga tcgttcatga ttacttggat ggtaaaggcg gaagtgatta tctggcaaag
     2581 aaatattcca ttaaggctcc atcacaagtg aagcgctgga tcaatgccta tcaagaattt
     2641 ggtgaagagg gtctagtaag aaagcgacaa aagaaaaaat attctgttca atttaagctg
     2701 gatgcgatag agttatatct aacaagtgag ctgtcctatc gtgaagttgc caacacactt
     2761 aacatgaaca accctaattt gatcgctagt tggatgcgtc aattccgtga gggtgggatc
     2821 gatggactct caaaaacgaa aggatgtcca cctatcttgt ctaagaaaaa tgaacccaaa
     2881 aataagaaaa aatctacaac taaagcaacg tctaaagaac gtgaacgcat cgaggagttg
     2941 gagaaacgag tccgttccct tcaaattgaa aatgcctttt taaaagaatt gaggaagctg
     3001 cgaaaacagg aagcccaaca acgacgaatg aagcaatcgc acgaatcatc gcaagcctcc
     3061 gaggaccgtt taaattagtt gagcttctag aaactttaaa gttccctaag gcaatcttta
     3121 tgtactggca aaaacgattg gaccgtaaaa atccagatca agagattgaa gcggaaatgc
     3181 ttaaagttcg ggagaagcac aaggattatg gctgtttacg gatgaccaat gaactaagaa
     3241 accgcggttt ctctatcaat aaaaagaaag ttcagcgcct gatcaagaag ctaggaataa
     3301 aagtaacgac atacacacgt aaatcacggc gctacaattc ttatcgagga caagt
//
LOCUS       DAZUMK010000187         3328 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000187 DAZUMK010000000
VERSION     DAZUMK010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.194"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2232)
                     /locus_tag="ACG808_002784"
     CDS             complement(<1..2232)
                     /locus_tag="ACG808_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS5048360.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="ACG808_002785"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="ACG808_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          913 a          745 c          537 g         1133 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgca
//
LOCUS       DAZUMK010000188         3228 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000188 DAZUMK010000000
VERSION     DAZUMK010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.195"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            166..855
                     /locus_tag="ACG808_002786"
     CDS             166..855
                     /locus_tag="ACG808_002786"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFS5048361.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            869..2371
                     /locus_tag="ACG808_002787"
     CDS             869..2371
                     /locus_tag="ACG808_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5048362.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            2384..2860
                     /locus_tag="ACG808_002788"
     CDS             2384..2860
                     /locus_tag="ACG808_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFS5048363.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(2957..3067)
                     /locus_tag="ACG808_002789"
                     /pseudo
     CDS             complement(2957..3067)
                     /locus_tag="ACG808_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1068 a          440 c          617 g         1103 t
ORIGIN      
        1 ctcatacaaa ctcctctaca atttcaactt ttctccatta tactccaaaa taataaaaaa
       61 aactcctaaa tactccattt ttatctttac ttttatgaaa gcggatgcta tattgttttt
      121 ggagttatta tccaaaaata ataaaaaatt ggagcgttta ggataatgga taaaaagagg
      181 tttttagatc ttatttcaaa aggtttgatt gtttcatgtc aagcattacc tggtgaacca
      241 ttttacaaag aggaagaaag tttaatgcct tattttgctt tggctgctca aagggctgga
      301 gcagttggta tacgtgcaaa ttctgttcga gatattattg aaattagaaa gtgtgtatca
      361 ttaccaatga ttggaattat taaaagagat tatccaccag aatctccatt cattactgcc
      421 actatgagag aagtagatga attagtagaa accggtgttg aggtaatcgc tcttgattgc
      481 actcttagaa atcgacatga tggactatct ataaattctt ttattactaa aataaaagaa
      541 aaatatcctc atcagttatt tatggcagat atttctacat ttgaagaagg caaaaatgca
      601 tataaagcag gaatagattt tgtaggaact actttaagtg gatatactga ggaaagtaaa
      661 aaacaagatg ggccagatat agatttgatt aaaaaattgg tagaagaagg gatttgtgta
      721 attgctgagg gaaggattca tactccagaa caagcaaaag agataaaaaa aataggagtt
      781 gcaggtattg ttgtgggtgg tgctattact cgaccacagg aaattgcatc aagatttgtt
      841 aaagcattaa gttaggggga gaaataaaat gtttaaaaaa ttttcacaat taggacgcgc
      901 tttcatgtta ccaattgcta tattaccagt agccggatta ctattgggac tgggaggagc
      961 gttaacaaat gagagtgcta taaatgctta tccattttta gatcaaccgt ggttgcatac
     1021 aattttaagt attatgagtt atgctggaaa tgcagttttt gcaaacctag cactaatttt
     1081 tgcaattggt attgctgtag gacttgcaaa tggagataaa ggaacagctg gtttagctgg
     1141 tggagtagct tatttagtat atactgcaac tattagtggg tttcttgaac ttttttcagc
     1201 aaaagatgca acactagata caggtgtagt gggatcaatt gttgttggtg gtactgttgc
     1261 atttttacac aatcgttata gaaaaattga attaccacaa ttcttaggtt tttttggagg
     1321 atcaagattt attccgattg tttctagttt agctgcaatc attattggtt ctttctttta
     1381 tttaatttgg ccaccaattc aaaatggttt agtagttgct ggtgaacaaa ttgcccaaat
     1441 gggtagttta ggaacttttt tatatggttt tttattgcgt ttaacaggtg ctgttggact
     1501 acaccacacc atttaccctt tgttctggta tacatcttta ggtggtaccg aaactgtagc
     1561 tggatcaaca attgctggag cccaaaatat tttctttgcc caactagctg atccaaacca
     1621 cactggtctg tttacttatg gtactagatt ttttgcagga agatttgcaa caatgatgtt
     1681 tggattacca gcagcatgtt atgcaatgta tcgtgcaatt cctaagaaaa atcgtaaaaa
     1741 gaatggtggg ctttatttta gcggggccct tacatcattt ttaactggaa ttactgaacc
     1801 agttgaatat atgtttttat ttgttgctcc ttggctttat gtgattcatg cattcttaga
     1861 tggattatct ttttattttg ctgatatctt aaatattcgt gtagggaatt ccttttcagg
     1921 tggtttgatc gactttttat tattcggtgt acttcaaggg aatgacaaaa ctaattggtt
     1981 aaaattaatt ccttttggta ttgcatgggc atttatatat ttctttgttt ttacattctt
     2041 tatcaaaaaa tttaaggtag ctattcctgg aatggaagaa gaatcagaag ttcattctga
     2101 aaaagtaata aacaaaaata catcattaca tgaagagtct cttatcatta ttgaagctct
     2161 tggtggaaaa gaaaatattg agtctgttga agcttgtgca actcgtttgc gtgttgctgt
     2221 tacgaatggg gagattattc aaaaacaaac aataaaagac actggtgcaa ctgctgtctt
     2281 cgaagtgaag ggtggcatcc aagctgtttt tggagggaaa gctgacttat taagtcaaga
     2341 aatcaatcaa atattaggaa aagataatta ggaggttttt aatatgtttg aaattttcaa
     2401 aaagaaaaaa caagaaactt tttatgcacc ttgttccgga aaaatcattt ccatcacaga
     2461 agtaaatgat cctgtctttt cacaaaaatt actaggtgat ggttttgccg tcatacctga
     2521 tggttcaaaa atttatgctc cattggctgg tcgagtaaca aatatatttc ctactaaaca
     2581 tgctattaat ataaaaagca actcaggcat tgattatttg attcatattg gaattgatac
     2641 agttgaatta caaggagagc ctttttcgat ttgtgttcaa gaaaatgatg aggttactcc
     2701 tgaaacctcg ttggttgatg tggattttga caaaataata aaatctggta aagatacttc
     2761 tgttatagtt gtttttccgg agaaaaatca gttcaatgat ttaataatag ataatggtcg
     2821 aattgctcat gggaattcat gtggaaaaat cactttttaa aaaagtgtat ataaaaagct
     2881 ataaaataag ctaaatagga tatttgtcaa atgaagttgg tgaacagaaa gaactgtttt
     2941 ctttctgttc atttttttat tttattagta atgattcccc atgccaaatc atgatccgta
     3001 atttaaagtt ataaaagctt cgaaatccat tggcatttcg ttggaatacc ttgatatgat
     3061 tattcaaata taaaatatag agagaagaga gagagggaag atatttataa agggcaaagt
     3121 tatgtttact caacttagga aatagaaata gatcaattta tattctcttg tcaatcgaat
     3181 atgaacctta gcactatata ccaaatatag taaaatgctg aaagatgt
//
LOCUS       DAZUMK010000189         3088 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000189 DAZUMK010000000
VERSION     DAZUMK010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.196"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(400..717)
                     /locus_tag="ACG808_002790"
     CDS             complement(400..717)
                     /locus_tag="ACG808_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS5048364.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            complement(718..972)
                     /locus_tag="ACG808_002791"
     CDS             complement(718..972)
                     /locus_tag="ACG808_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS5048365.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFRDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            complement(1232..1372)
                     /locus_tag="ACG808_002792"
     CDS             complement(1232..1372)
                     /locus_tag="ACG808_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin RelE"
                     /protein_id="HFS5048366.1"
                     /translation="MYRVASEIQENKVIPVLLYGTHNNFYELAENTRNFIRVDKSASV
                     EL"
     gene            complement(2010..2096)
                     /locus_tag="ACG808_002793"
                     /pseudo
     CDS             complement(2010..2096)
                     /locus_tag="ACG808_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT         1027 a          497 c          428 g         1136 t
ORIGIN      
        1 catagatata ttggacttcc tcttttaaaa cacaaaaata aatactacct tatttaggta
       61 gtatttatta aaagttacgt caattttgat taaaaatata ctacttgaac ctctcatgag
      121 taaagtcacg aaatttttgg gaatagagcg tacttgatat cttatttttt cactaatagc
      181 agtgtctctt attcaccaag cgatccccgt agttcctacg gttcttaatt tttctctaga
      241 ctaatgattg tatctttaga ccttcggcca taacatggag caaaataaaa aggtactaag
      301 tcttatatat caatcttcga gtaaccgatg gttaacaacc atatcgagcg gcattaatca
      361 cccacctgta gaggatgagc gacttttgcc taattattgt taaaagccta gtttcatttg
      421 tttagattgg aaaatacgtc ctacaagtat ctcttttttt tcatggtcga tccgataaaa
      481 taaggcatag cttttgccga tcacgatccg atatgttggg atatccattc catagatttc
      541 ggaaacttcc tcatgcatag ttggaaaatt tttggttagt tctaaagcct tctggatcgc
      601 acctacaaat ctttttatgc tggctggaga aagagcaagc tcattttccc attcttctat
      661 tatcgtttca aggctggtct ggaaactttt agaataaact agcgtgtact ctttcatcta
      721 ttcactccag ccgtattttc cgaaagcttt taccatttca atttctgaaa ctgttaaacc
      781 tttctcaaag tctcggaaac tttcttcaat caatttgcga tcgtgttctt ttgctatttg
      841 agtagtatct aatatgaata agttgccttc ttgtttggct tggatagtgt ctccgctttt
      901 taatccaaga gagcttggaa ggatcactcc cacgctattg ccgatctttc taacctttag
      961 ttcttttccc atgttatcac actcctttta gtttttgata aataaattat aacatgttat
     1021 aacaacaatt gccaacggtg tttcttaagg gattccactc catattatta ttggacaaaa
     1081 taagccactt agaacgctga aaacatatac aaacgatatc aaaatacgag tgaactactc
     1141 accatacact tccgttaaca tccatttaag taggtatttt tattatatat agatttaaca
     1201 tatgtcggtc ggttgattga tatagctaaa gttataactc aaccgaagcc gatttatcta
     1261 cacgaataaa gttgcgagtg ttctcggcta attcataaaa attgttatgt gttccatata
     1321 gcaatactgg aataacttta ttttcttgaa tctcagaagc aactctatac acggtgaccg
     1381 catacttaaa tccattagac caattacctg ataaacctcc agttttttgc tcttacttaa
     1441 atggatcaat agaaatttca tcacaaacct atagaggatg agtgacttct gcctaattac
     1501 gttaaaaaat attgaaagta actctatgta gcaaaaaata tctagaattt catttcctca
     1561 aaactgatcg atattggatc caaaaatata tactacctaa aataggtagt atatattaaa
     1621 atatgtgctt ttgttagaaa aatcaataca taaattatca tatctgtata tgtcatttag
     1681 gtatttcagt tatgttataa tagttttgct gtctgctacc ccggcctaat ctggcaaggg
     1741 gaggaggaga tataatgaaa tacctttttg attttgtttt tgcaatcaag ccatttgcag
     1801 aggtaactgc acactacatc tgcaagtggc ttgattgcaa aattgccaaa aaagacagta
     1861 agcctacccg ttaattctat aatatagaaa cgtaaaaaaa accccatcag cctgcacgct
     1921 gatggggttt ggtgatataa tgaaatacct ctttgtaact cataattata ccataattag
     1981 tcacgaatgc aactaataga aaaaactact tagaacggta gatcatcgct aataacaatg
     2041 cattgtccac caaatggatt ttgtgaagca ttaaatccat tgttattaat tgtcgctgct
     2101 tttaactagg cctctcccct tctattataa aagaagatat atatcatttc ttaattacat
     2161 actaccttaa accaaaattt aagtactacc taatttaagt agtacttaaa taatctaaaa
     2221 aatagttatc ccaattgtta tgcttttata atacgattat ttgtaacaaa atgaatatta
     2281 tataattaaa cactaccata tttaggtagt gtttaattat tagcaaaaca aaaaatattt
     2341 attctattct cttaacttta actaagttat gctgcacatc tttatataaa tcatacgcaa
     2401 ctcattagct agacaaacta caagcatgta gttttatctg ttaattatgt actacctaaa
     2461 taaagtagtt tataattaac aagtatatat atttaaaaat ttaaaattaa tttttttatg
     2521 acttgtacaa atttatctga tattctctta ttaagaacta cctaatttgg gtagttctta
     2581 ttttttacat tttaagtagt atttaattgc aatttggtag tattttttta ttatcttatt
     2641 cctagaacgg tagtacttaa tataaatata ggtagtatct aatttaagta ttaatggtag
     2701 tatataattt aacaggtagt atgtaactac tttaaagtaa tttttttcag attgacacag
     2761 tagtatctta ttaaataagt taattttaaa ggtagtatat aattattata tatatctaac
     2821 attggtagta cttattgtat tttttagtag tatataatct attgttttat agcctaaagc
     2881 cttgtctcat aagaataata taatatatac tactgaaata aggtagtata tattactagt
     2941 aatggtagta tgttttatat gcataaggta gttcttaatt tattttttgt tttgacactg
     3001 gtagtactta attatcctta attttaaaac taaattttat tcatacgttc ttctggtagt
     3061 atttaattat aaataatatt tatttaaa
//
LOCUS       DAZUMK010000190         3079 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000190 DAZUMK010000000
VERSION     DAZUMK010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.197"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            84..279
                     /locus_tag="ACG808_002794"
                     /pseudo
     CDS             84..279
                     /locus_tag="ACG808_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
     gene            complement(1811..2329)
                     /locus_tag="ACG808_002795"
     CDS             complement(1811..2329)
                     /locus_tag="ACG808_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFS5048367.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            2696..2944
                     /locus_tag="ACG808_002796"
     CDS             2696..2944
                     /locus_tag="ACG808_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048368.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT         1051 a          536 c          447 g         1045 t
ORIGIN      
        1 cggttgattt gatattaaca agcgtatatg aatgtatata caaataaaaa aggggaggga
       61 gaagttaaag aaaattcaga gaagtggggc actcagtagt attaacaatt taaaaggaat
      121 ttcttaaaga aaatgggttg catcctggag atacaatttt tttagataaa gaaaaaattc
      181 aagcaacaat tcaaaaaaat cttaactgct gagattgatg tagcaatatc tcaatcatca
      241 acaaagtacg atcctgcctt tcaaaagctt attgataaat gtttattcat tataaaaata
      301 aaagaaaaat ttatgtcagc ttttttcatc aaagtggtaa tgataattcg tctattaatt
      361 gtatttttta ggaaatatgg tataattgtt ttaacgaaaa ggtaattcat gatacaccaa
      421 aaaccactag ctggcgggct agtggttttc ttttacgttc tattcagatt aacgggtagg
      481 cttactgtct tttttggcaa taattttgca atcaagccat ttacagatgt aatgcgcgga
      541 tacttctgct aagattgcaa aaaacatatc gaaaaggtaa ttcataatac aacctcctcc
      601 ccttgccata ataggccggg gtagcagaca gcataaatat tataccataa ctgaaaattt
      661 cattttagta catttcaata tattgatgaa ccagcacaat aaaacatgcc atgactaaca
      721 gaaaaaaact tattaaacca gatataactt gtaaacatcc aactatatag aaaacgtaaa
      781 ttttctatat agttggatta agataacgac tatttatttt atcatatttt tactataccc
      841 tatactttta cttggtatat ttatgaataa tcgcaaattt acccctatac tactagtttg
      901 taagttagat taataaaaat actaaagtta tttgttcttt tattaaatac acctacaact
      961 tataaattga tggtatttag ataacacgaa agaataaaaa taacagctga tagtaaagtt
     1021 tgttgacgat tgctactcaa tcgaaagtga gttagtgcgt atgaacttgc ttctgaaaac
     1081 ttatgaaatg aaagaccttt tacatcttgc agaaatcgtt actgggatta aactaagcaa
     1141 taaaaactaa tgttcccctc attctttgaa cttctactat ttgatttatt tattctgata
     1201 aatgacgatt tcaccttcag gatcttcaaa tcccgtatag aatttattta agatctcttc
     1261 agtatcagta ataggatagg cgataggcca catacccttt gatggttaga ataaactgct
     1321 caaaatctgt atcgtatgtt ggctttagca tattaacaaa ggctatctcc aaattataaa
     1381 ccataagcgt tgaatcgttc ttaacaattt caattcttcc taacccaaag tcagaagata
     1441 taattaccgg acttgcctta cccttcatgt gggttgttga gattccatat ttaaagggaa
     1501 ttgtcggtac tatcggcggt ctgaaaaaca tcccaaaaat aaaaaaatct ttcccctgct
     1561 agtctcctaa gtccctttgt agcggatatg gcaccataat ctcattttat cgaagtgagt
     1621 catggtttat ctgttggcca accaaaattg atacaatcta acgatgcacc ctctacggca
     1681 ttctagggga gtgcacttgc attcacccaa taaaaaccgg cagttagcaa agaacttatg
     1741 tagtcgtatg ttcttactgt taacatacaa gaggtctcct ttccacaatt gcttagcagt
     1801 aaacgggaat ttacaaagct aatctcttcc attttcttat ctttgttcta aaagtcccaa
     1861 atggagcaac tgtatttaca tgaataaatt tatatacttc ccatacagcc gttttggttg
     1921 cctcgtctgc ccattttctt ttgtgtgcag tgaatagatc ttcatcactc attgaatcaa
     1981 tcatcatgtt aatatcatca atattacgat tgagaatttc ctctaattct ttcaatggta
     2041 gatgtgcata ggtatttgta aaccacttat ataactctcc taactgattc catttaaatt
     2101 gatcagaagg cgttttaaca gataatccat ttctttcatc attttcccat tttagaatta
     2161 aattcgtcca accaacttga tatgctaaat tttcagcagg tgttctatcc acttcctcaa
     2221 ctcttttatc cttcaattct tcaggaatag tttcaaattc taaaatatat ttagtataag
     2281 tcctatttat ttcttttttt aattcttctt tatttttata tgttctcatt atattacctc
     2341 cataaattcc taattgtctc ctttaattca tctataattt catccacgat attttaatcc
     2401 gttctacaat gcatatctga tatgcattga ctgtctaact catatgtgaa tgatttataa
     2461 aatatcccaa cgtctcatct aaagaaatgt tgatcgtcag caaggagcta aaaaatacga
     2521 atatatttct tttgtagtaa aatcggactg gtttgcatat cattttcttc gtaaatatca
     2581 aaatcatgtt aactactatc aattactaat aaaaactacc actttaagcg ttaagaactg
     2641 ttaagtttag ttaagtgagg atagttcaca aaatccattg ttaaacttca cgaaagtggt
     2701 aaaactcaaa atgctttata taggattaca gcatcgctct ttcttcgatt gcgcgttggg
     2761 taaaaaatat tatgaagtaa aaattgacga tgacacgttc cttacggctc atcagatgaa
     2821 acagctacaa aaacgaaatg atcaactcga agaaaattta attctaaaaa agcgattgcc
     2881 atattcacgc ctcgctcgac gaactattaa aggtcaagat ttacctgaag atcatccttt
     2941 ttaggaaatt gaagcttttt atgttgcaca atgcatttat aatactacaa tgatgttttc
     3001 accagatatt cttctagggt gcggtgtaat gaaacagata cacttgcaaa agaaaattca
     3061 ccgcgaattt actaaaatt
//
LOCUS       DAZUMK010000191         3063 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000191 DAZUMK010000000
VERSION     DAZUMK010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.198"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(105..1562)
                     /locus_tag="ACG808_002797"
     CDS             complement(105..1562)
                     /locus_tag="ACG808_002797"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFS5048369.1"
                     /translation="MGNNIHGKTAKALKWSSLAEIIAKIITPISNMILARLLTPDAFG
                     VVATVTMVISFADMFSDSGFQKFLVQHEFDNEEHMFSSTNVAFTSNLGISLLLWLFIC
                     VFNNKIAHLVGNSGLGLVIIVAGVSLPITSFSSIQSAIFRRNFDYKILCYARIISALI
                     PLVITVPLAIIGLSYWSLVIGSIIGNLVTAAILTKLSEWKPKLFFSKKEFYEMFSFSA
                     WSLVESLGTWLSSYIGTFIVGGILSVYYLGIYKTTMTTVNGIFAIVTSATTTVLFSTL
                     SRLQNSKTEYDKTYLDFIKIVSIFIIPLGIGIYVYRELVTNILLGSQWKEAVPFVGIY
                     GLMSCFTLVLGQYASEYYRGLGKPKANVLMTILHLAVLIPVLIIYSRKGFIELAYGRS
                     FVKIEQIIVFWIILWFGFKFNPFKILKQVWKSCASALLMGVLAKIMLEFCTNYVMQLL
                     SVCLCVILYFFIYSCIFKGKTEIINMVRMFTGKGY"
     gene            complement(1621..2082)
                     /locus_tag="ACG808_002798"
     CDS             complement(1621..2082)
                     /locus_tag="ACG808_002798"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFS5048370.1"
                     /translation="MRRKAEKWADKACRQYRNGQNKMSKISCYILRIIYGCEFYGQVD
                     FSSNAQLVHNGLGCVFHPKTKIGNNCKIYQNVTLGGNGKIINGKETVGAPTLEENVAV
                     FAGACVLGPINIGHDSIIGANAVVTKSVPPYSLVYGNPAIITELKFDYKFD"
     gene            complement(2093..>3063)
                     /locus_tag="ACG808_002799"
     CDS             complement(2093..>3063)
                     /locus_tag="ACG808_002799"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFS5048371.1"
                     /translation="IILFLFICFGISTSMFLPFLIDALKQLMTISLFICSFLIGKRFK
                     MYSKNLTMIGWNFYFIGVVSMAFQVYLQRVYILSTGVIIGHYAEMGLNRIAYAGLMGD
                     FSFAGLYLATGCLYAFLKYVDLKNWKLLFFIIIEVFLLGAILCVSARTGLVSLFVTMT
                     LYYLFNINRISVPHLLVLIGGIITTPYILVMLMAGRSGQSLLDSSGRLENYMSSLNAF
                     SDKYLIGYGLGLNNLYTLLGVAVPHNFFIQYLLQTGIIGTLIILSGFFKFTYEELKKS
                     GCYKWLFLMIVVGAMFIPDIVSSRFLYAIVVLCMISASERTIKKES"
BASE COUNT         1133 a          572 c          347 g         1011 t
ORIGIN      
        1 gcgttataaa agctactctt tccattccac cataatacat tccaggaatt aaaattgtta
       61 cttttttcat tttcctaatc acaaccttgc cataaaaatc tctcttaata tcctttacca
      121 gtaaacattc taaccatatt aattatttct gtttttcctt taaatataca actatatata
      181 aaaaaatata gaattacaca aagacacact gaaagtaatt gcataacata attagtacag
      241 aattccaaca taatttttgc tagaactccc attagcaatg cactagcaca agatttccaa
      301 acctgcttta aaattttaaa tggattaaac ttaaaaccaa accataaaat aatccaaaag
      361 actataatct gctctatctt aacgaaagat ctaccatatg ctagttctat aaaccctttc
      421 cttgaataga ttattagaac tggaattaat actgctaaat gtaggatagt cataagaaca
      481 tttgcttttg gttttcctaa accacgatag tactcagagg catattgtcc caagacaaga
      541 gtaaaacagc tcattaatcc atatatccca acaaatggta cagcctcttt ccattgactc
      601 ccgagtagaa tatttgttac tagttcccta tatacatata tacctatacc cagaggaata
      661 ataaaaatgg aaacaatttt aataaaatct aaatatgtct tatcatactc cgttttacta
      721 ttttgtaaac gagataatgt tgaaaataat acagttgttg ttgctgaagt tacaattgca
      781 aaaataccat taaccgttgt cattgtagtt ttgtatattc ccaaatagta tacgcttaaa
      841 atgcctccta cgataaaagt acctatataa gatgacaacc atgtacctaa tgattccact
      901 agtgaccacg cactaaagga aaacatttcg taaaattctt tttttgaaaa aaaaagtttt
      961 ggtttccact ctgataactt agttaatatt gcagctgtaa ctaaatttcc tattatagat
     1021 ccaattacta gtgaccaata acttagtcca attatcgcca acggtactgt aataactaaa
     1081 ggtatcaatg cagatattat tctagcataa cacaaaattt tgtaatcaaa atttcgtcta
     1141 aaaattgccg attgtatact ggaaaaagaa gtaattggaa gagaaactcc agctactata
     1201 attactaatc caagtccaga atttccaact aagtgcgcta ttttattatt aaacacacaa
     1261 ataaataacc ataataataa agaaatacct aagttactag taaaagctac atttgttgat
     1321 gaaaacatgt gttcttcatt atcaaattca tgctgtacta aaaatttttg aaatcctgaa
     1381 tcactaaaca tatcagcgaa actaataacc atagttacag tggctactac tccaaaagca
     1441 tctggagtca ataatctagc taaaatcata ttcgaaattg gagttataat ctttgcaatt
     1501 atttcagcta aggatgacca ttttaaagct tttgctgtct ttccatgaat attatttccc
     1561 atttattacc ccattttgct aaataaatat agcaaccttt cgcatattaa acattacttt
     1621 ttaatcaaat ttataatcaa atttcaactc tgttatgatt gctggatttc catatactaa
     1681 gctatatggt ggaacacttt ttgtaactac agcatttgca cctatgattg aatcatgtcc
     1741 gatattaatt ggtcctaaaa cacatgcacc agcgaaaaca gccacatttt cctctagcgt
     1801 tggtgcccca acagtttctt ttccatttat tattttgcca tttccaccca aagtcacatt
     1861 ttgatatatt ttacaattat taccgatttt tgttttaggg tggaatacac aaccaagacc
     1921 attatggact agttgagcat tagagctaaa atctacctgt ccgtagaatt cacatccata
     1981 aataatcctt aaaatatagc aggatatttt tgacatttta ttttgaccat tcctgtattg
     2041 tctacaagct ttatctgccc atttctctgc ttttcttctc attttttcta cctcaacttt
     2101 ctttttttat agttctttct gatgcagaaa tcatacacaa aacaactatt gcgtaaagaa
     2161 aacgcgaaga tacaatatcc ggtataaaca ttgctcccac tactatcatc aaaaataacc
     2221 atttatagca acctgatttc tttaattctt catacgtaaa tttaaagaag ccacttaaaa
     2281 taattagcgt accaataatt cctgtctgta ataaatattg aataaaaaaa ttatgtggta
     2341 ctgctacacc tagtagcgtg tataaattat ttaatcctaa tccatatcct attaagtatt
     2401 tatcactaaa agcatttaaa gaggacatat agttttctaa tctgcctgaa gaatccaaaa
     2461 gactttgtcc agaacgacct gccattaaca ttactagtat ataaggtgta gttatgattc
     2521 cacctattag aactaaaaga tgaggaacac taattctatt tatattaaat agataataca
     2581 atgtcattgt gacaaacaag gaaactaacc ctgttcttgc actaacacat aaaattgcac
     2641 ctaataaaaa tacttctatt attataaaga ataataattt ccaatttttt aaatccacat
     2701 attttagaaa agcatataga cagcctgtag caagatataa ccctgcaaaa ctaaaatctc
     2761 ccatcaaacc tgcatatgca attctattta aacccatttc tgcataatgg cctattatta
     2821 caccagtaga taaaatatat actcgttgca aatatacctg aaacgccata gatactactc
     2881 ctatgaaata gaaattccac ccaatcatag ttaagttttt gctatacatt ttaaaacgtt
     2941 ttcctattaa aaaactacag ataaacaacg aaatagtcat taattgcttt aatgcatcta
     3001 ttaaaaaagg taagaacata gacgtcgata ttccaaaaca aataaataaa aataaaataa
     3061 tca
//
LOCUS       DAZUMK010000192         3056 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000192 DAZUMK010000000
VERSION     DAZUMK010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.199"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1010
                     /locus_tag="ACG808_002800"
     CDS             <1..1010
                     /locus_tag="ACG808_002800"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS5048372.1"
                     /translation="FDQERFLKTQAGAVATNEQISNILESIASDLEEIYFIGSGGVGI
                     LFDSTIEFARTRSTFPFHRVIAAEFMIHPNKTFSNKSLVVIPSLSGTTKETLELVEYC
                     NEMNVKTLALVGSPKTPLEKAATYSIYNDVLDDTSSENYYLQGLLIALFLMKQRNEID
                     INQYNTYIEDLKKIPEALVHAKEQAEPMAKSFAKKHKDTSYHILTGAGDSFAEAYYYG
                     MCILEEMQWIKTRPVHAADFFHGTLELIEAGVSVIITEGEDDSRPLTERVKNFVPKFT
                     DVHVTVDTKDYDLPGISEDTRKIISPVVLATVFERISAFLEEERNHPLTTRRYYKQME
                     Y"
     gene            1118..1987
                     /locus_tag="ACG808_002801"
     CDS             1118..1987
                     /locus_tag="ACG808_002801"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFS5048373.1"
                     /translation="MKIAVICSGGDSPGMNSALLAIKKTIDSEPDSEVVFFKNGYAGI
                     FKECIASVSLKEVEVFKAGTLIGTARFEKMRDLESRKIIIENLNKMEIDNLIVIGGNG
                     SLKGAELLKNESELQVSVIPGTIDNDFPNTDYTLGFDSALNTIIRSIDDLTSTANAHD
                     RIIIMEVMGRGCFALPEYASIANNTYWVERDDKEYLHLICKKFESEFYSPILLLPEGI
                     SNKQKIIEFITKNLPKETKYLNLAYLQRGSSPTFYDRYIGYLWGQLAAKELIEGKSGI
                     LSQKNGNIMLIPW"
     gene            2002..2706
                     /locus_tag="ACG808_002802"
     CDS             2002..2706
                     /locus_tag="ACG808_002802"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5048374.1"
                     /translation="MIFDKVKEQIRKVYITTDRKSELLPSERDLAQEFSVSRPTIRKA
                     LASLEDEGIISKNEGRGYLVKPASEKYVDHELDSFIGFFQDAVQQGKKISSKIIQQTI
                     IPADSTISECLEIEQNTLVLVLERIRYIDDYPTCIAKSCIPLSVLPEIVEHDFAKESL
                     FSVLESRGIRLAYAKRSIEVTPPTDLENLYLDLEKNEPVFVFTSIGFAKNQKPFEYEI
                     SKYPAYKTKFESTVSK"
BASE COUNT         1026 a          545 c          548 g          937 t
ORIGIN      
        1 attttgatca agaaagattt ttaaagactc aagctggtgc cgttgcaaca aatgaacaaa
       61 tttccaatat tttagaatcc atcgcatctg acttagaaga aatttacttt attgggtctg
      121 gaggagttgg tattcttttt gattcaacaa tcgaattcgc gagaactcgt agcactttcc
      181 cttttcatcg tgttatcgct gccgaattta tgattcatcc caataaaaca ttttctaaca
      241 aatctttggt tgttatccca tctttatctg gtacaacaaa agaaacactt gagttagtcg
      301 aatactgtaa tgaaatgaac gtcaaaacat tagctttggt aggaagtcct aaaacgcccc
      361 tagaaaaagc tgcaacatat tctatctata acgacgtttt agatgataca tcttcagaaa
      421 attattacct tcaaggactg ctgattgctc tcttcttaat gaaacaaaga aatgaaatcg
      481 atatcaatca atacaatacc tatattgagg atttaaaaaa aatacctgaa gccttagtgc
      541 acgctaaaga gcaagcggaa ccaatggcaa aatcttttgc aaaaaaacac aaagatactt
      601 catatcatat tttaaccggt gctggtgata gctttgcgga agcatattac tatggcatgt
      661 gtattttaga agagatgcaa tggattaaga ctcgtcctgt tcatgcagca gacttcttcc
      721 atggaactct tgaattaatt gaagcgggtg taagtgtcat cattacagaa ggagaagatg
      781 attctcgacc tctcacagaa cgcgtgaaga atttcgttcc taagtttaca gatgttcacg
      841 taacggttga tacaaaggac tatgacttac ctggcatttc ggaagacacg cgaaaaatta
      901 tttccccagt cgtcttagct actgtctttg aacgaattag tgcttttcta gaagaagaac
      961 gtaaccaccc tttaactact cgccgttatt ataagcaaat ggaatactaa tcataaaaat
     1021 atgaaaaagg tgaccgcgac tccattgccc atttgattgc ttcattttct ctaatgggag
     1081 aatagaatcg cagtcacctt ttttaggagg atctaaaatg aaaatcgcag ttatctgtag
     1141 tggtggcgac tctcccggaa tgaattcagc tttattagca ataaaaaaaa caattgatag
     1201 cgaaccagat tcggaagtag tcttttttaa gaatggttat gccggaatat ttaaggaatg
     1261 tattgcctca gtttctctaa aagaagttga ggtattcaaa gctggaacac ttattggaac
     1321 agccagattt gagaaaatga gagatctcga aagtcggaaa attattattg aaaacctaaa
     1381 taagatggag atcgacaact taattgttat tggaggtaac ggttccttga aaggagctga
     1441 actattgaaa aatgaatcag aattacaagt atctgtcatc ccagggacta ttgataacga
     1501 tttccccaat accgattata cacttggctt cgatagcgcc ttaaacacta taatccgatc
     1561 aatcgatgat ttaacttcta ctgctaatgc tcatgatcgt attattataa tggaagtcat
     1621 gggccgaggc tgctttgctc ttccagagta cgcttccatt gcaaataata cttattgggt
     1681 tgagagagac gacaaggaat acttacactt aatatgtaaa aaatttgagt cggagttcta
     1741 ctcgcctata ttgcttttac cagaaggtat atctaacaaa caaaaaatca ttgaatttat
     1801 taccaaaaat ttaccaaaag aaaccaaata cttaaatctt gcgtatcttc agcgtggatc
     1861 atctccaact ttttatgacc gatatattgg ctatttgtgg ggtcaattag cggcgaagga
     1921 attaattgaa ggaaaatctg ggatattaag tcaaaaaaat ggtaatataa tgttaatacc
     1981 ttggtaaaag gatggattac tatgattttt gacaaagtaa aagaacaaat tcgaaaagtg
     2041 tatatcacca ctgatcggaa atctgaatta ttgccttcgg aaagagattt ggcccaggaa
     2101 ttttcggtga gtcgtccaac aattcggaaa gctttagctt ctttagaaga cgaaggaatt
     2161 atttctaaaa atgaaggtcg cgggtatcta gtgaaaccag cttcagaaaa atatgttgac
     2221 cacgaactcg actcttttat cggattcttc caagatgcgg ttcagcaagg aaagaaaatc
     2281 agttctaaga ttatccaaca aaccatcata cctgctgatt caacaattag tgaatgtttg
     2341 gagattgagc agaatacttt agtgcttgtc ttggaaagaa ttcgatacat cgatgactat
     2401 ccaacttgta ttgcaaaaag ttgcattccc ctatctgttt tacctgaaat tgttgaacat
     2461 gattttgcca aagaatccct gttctcagta ttagaaagta ggggaattag attagcttat
     2521 gccaaacgct caattgaagt tacaccgcca acagatttag aaaaccttta tcttgattta
     2581 gaaaaaaatg agccagtctt tgtctttaca agtattggat ttgctaaaaa tcagaaaccg
     2641 tttgaatatg aaatatctaa gtaccctgct tataagacaa agtttgaaag tactgtttcc
     2701 aaataatttt taaatatgct taaatgtata aagagcctcg cggataacag ataaagtgtc
     2761 tttccgcaag gctctttttc tacttcctct ttatcttaat ccgctaagta gcctacagtc
     2821 caaacattat catctaatta aggtgtgtgg aggtactcct ctaccctaag tcaaggatag
     2881 tcgacatagt tgttaataag tcttcaaagc aagcctgaac ctttttcatt aggtgtgctt
     2941 acttcacata tgacgctatc aaaaatgata gcacccgttg aatcaaccaa ttttctctaa
     3001 ttcatatccc cattcaaaca actatatttt aaaaactttt ttaatctttg attatg
//
LOCUS       DAZUMK010000193         2985 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000193 DAZUMK010000000
VERSION     DAZUMK010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.200"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            76..627
                     /gene="lepB"
                     /locus_tag="ACG808_002803"
     CDS             76..627
                     /gene="lepB"
                     /locus_tag="ACG808_002803"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFS5048375.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(710..1252)
                     /locus_tag="ACG808_002804"
     CDS             complement(710..1252)
                     /locus_tag="ACG808_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFS5048376.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(1489..1887)
                     /locus_tag="ACG808_002805"
     CDS             complement(1489..1887)
                     /locus_tag="ACG808_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5048377.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            2159..>2985
                     /locus_tag="ACG808_002806"
     CDS             2159..>2985
                     /locus_tag="ACG808_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS5048378.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHFLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIR
                     CSARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFK
                     SITFDCGKEFSNWKQISNANDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     "
BASE COUNT         1050 a          517 c          515 g          903 t
ORIGIN      
        1 tatctagtcg catttttttg atatttttct tataataaag aaagaatgaa aaaaagaggg
       61 atggattgat agagattgac aaaaaaacaa cgctatatta atcgcttctg gcttgtattc
      121 aaatacttac ttgtttctgt attccttgcg ttcatgttgc ggggattttt attcatcccg
      181 gtaccagtgg aaggcaattc aatggaaaac gtgttgaagc aaggtgatat ggttgtgatg
      241 gaaaaatttt cggagatcag acgatttgat attgttgttt ttcaattagc agatggcacg
      301 atttatatca aacgagtaat cggtcttcca ggagaaaatg tcagctacca aaatgatcaa
      361 ttgaagatca atgggaaagt agttaaggaa ccttatctaa ctaaaaacct gaaatcagat
      421 catgcaaatg catcctatac gacggacttt acactacaag agctaacggg acaatccaag
      481 cttccggaag ataattattt tgtacttgga gataatcgaa gagtttcaaa agatagtcgt
      541 tcgtttggca cgatcaataa aactgatatt ctaggtaaag caagatttgt ctattatcca
      601 cttgatgaaa taaaatggat ccagtgagaa acaataaagc gtgcagtctg ttgagactgc
      661 acgctttatt gtttaatcca aaatcagttt actaccttag tacgtaaaat caaacagctc
      721 gtttaaacac ttttctagcc tctaaagttt tgataaatga ataaacaacc attatttgaa
      781 tagggatttg gatcatattc ctgatgaatc tacctagaaa aattactttg aaatctgcac
      841 caaacaaaac agacatcagc caagaattga taaaaaggct gactgggatc ataatcaata
      901 attgggcagc gatgacggaa ggcaggttta tctttttatg atataaaaat aagccattaa
      961 taaatccacc aaggaaagcc gtgataagaa aagcagcttg aaacgttcct gaaccaaata
     1021 tcgtgaaatt taaagtgttc gacaacattt caagcaaacc agcccagatc ggtccatata
     1081 gataacccat aagcgccata cttaaaaatc cgaatgtgat ttgaaaataa gcaggaccaa
     1141 tcgctatctt gtataagatg acatcaaggg ctaataaaag agcagaggtc gacaacaatc
     1201 ttgcattcat ttcgggcaat ctaaaaacgt cttttgcaat caaagtgttc aaaatattct
     1261 ctcctttgtc agttaataat aaaaggatac cataaaatca ccaatgtgtc atgacacatt
     1321 ggtgatttac atacactggc atctaacaat taaaaaaagg gtgagaagac tgcttttata
     1381 aactgtaaaa aagagattgt acgaataaaa aaaatagagg cggagaatac ttatcgtatc
     1441 atccgccttt gtttttttgt tataaaaaac gtcagattag ttatctgctt acatcttcat
     1501 ccatacgggt tctaatgtat ttccgtctag atcttggact tctaatgaaa acatttcttc
     1561 ttcaggcatt cccatatcta cttcgtggaa agtaccacca ttttcttttg caatttgagc
     1621 aaattttttt acttcatccg cattttcaac actaaacgaa atcaaagcac cacttgtttt
     1681 tttattatca gctatttctt tatcttttat aaatttttta taaaaatcat gagagagaag
     1741 cattatccaa aaagtgtcat cccataccat tgaaccagct tcttcagtag aaaattcttc
     1801 atttttttta aaacccagct tttcataaaa agcaacggaa cgattgacat cagcaacagg
     1861 aaaattaaca aaaaccatac tattcatttg tttcgtctcc tttctgtttt gttcgtctat
     1921 catctcttat ttaggaaacg ctgtcaaaaa tttagattca tatatagaaa ccaaaataaa
     1981 aatttgcatc atcaaaaaag tgaagatgtg atagaatgag gactggttca ctttcactta
     2041 actaatagat ggaatttctt tataacgccg attgtaaaat taagctagac aactaaaaaa
     2101 gtcatttgtg ctacactcaa aatagttccg tccaaagaat tataaggagt gagtacaaat
     2161 gacctatacc catcttacaa cggatgaact tgtaatgata gagtcttatt tcaaaataaa
     2221 tcaatctgtt gcgaaaactg cgcatttctt gaatcgttca agacaaacga tccataaagt
     2281 atacctgttc ttcaagcaag gaaaatcagc cttagagtac tatcaacagt ataagaaaaa
     2341 caaatcaaac tgtggtagac gcccgcttgt tttacctgag gaacaatcag aatatattca
     2401 aagaaaggtt gttcaaggat ggacacccga tgtgattgtt ggtcgtgcag cgtttcctat
     2461 tcgttgttct gctcgtacca tttatcgtat gttcaaaaag gggctattta atccttctga
     2521 cttaccgatg aaaggcaagc gtaaaccaaa tggacatcaa gaaagacgtg gaaaacaagc
     2581 tttccgtcgc tctattcatg aacgtgaaaa agattatagc caattctcaa atgagtttgg
     2641 tcaccttgaa ggtgacacta tcgtaggttt gaaacataaa agtgctgtaa ttaccttagt
     2701 tgagcgatta tcaaaagtta tcatcacatt gaaaccgtgt ggtagacaag cgattgatat
     2761 tgaaaacaaa ttaaatcatt ggtttgaatc tgtaccgaaa aatctattca aatccatcac
     2821 ttttgattgt ggaaaggaat tttcaaattg gaaacagatc agtaatgcca atgatattgc
     2881 catttatttc gctgatccag gaacgccgtc tcaaagaggc ctaaacgaga attctaacgg
     2941 attgttacgt agagatggtt tattgaaatc tatggatttc aattc
//
LOCUS       DAZUMK010000194         2947 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000194 DAZUMK010000000
VERSION     DAZUMK010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.201"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(449..865)
                     /locus_tag="ACG808_002807"
     CDS             complement(449..865)
                     /locus_tag="ACG808_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048379.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGILIGTNSKVYQETKEKI
                     EKQLNPSKRNQPKNRQKETNQTFTSKNFDPTNFLKR"
     gene            complement(867..1148)
                     /locus_tag="ACG808_002808"
     CDS             complement(867..1148)
                     /locus_tag="ACG808_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048380.1"
                     /translation="MDILVRKIDSKVIAKIDKRCQELTNKTGKKWSRNRYLKVLIEND
                     FDHALMHYKKDQFDLLLEKFIAIQEKNTETLNEYVRTNNQLIETLIGGR"
     gene            1948..2922
                     /locus_tag="ACG808_002809"
     CDS             1948..2922
                     /locus_tag="ACG808_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFS5048381.1"
                     /translation="MSKRGTIINEWEKLNRISNRRLNAQTMCTPLKMQWSIDRITIVG
                     TLRENIYYHTPHDVLILDFEQLMRLNEGNGYLKAVGNNGWQLLDQHEENIAYIEILKF
                     QEGKGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKVVPKEIDTWWRLELQ
                     LRRGKATDWHAMVHESLDSFASPHYLPEDVKVTDRMMITALITEHSYWGQIHRNSKYK
                     YRELLKQESQNDELTNHLRETFSESADDLKRELDTWLQGLDVTEVGAE"
BASE COUNT          913 a          438 c          603 g          993 t
ORIGIN      
        1 aaaaaaactt gctgatgaat tgggtgtatc gaaaaatacg gtgcgcaatc aactggtcaa
       61 ttcgggattt gaccttgctc agtataaaga aaaaaatgca gtggtcaatt gaccgaaatt
      121 tatgtctttg ggttttgggg ttttaaaacg aaaaaaaggg ggcgattttc gccccctttg
      181 ctctatgtga aaaatttgtt atacttattt tatccttaaa acataccaat ctttttctta
      241 tttagctgct cgtacgagca gcttttttta cgattatgaa ttcaaatcat gtatttataa
      301 tttttttgtt tttatctttt tttctgttat ttcttctgaa attcgttttt ttcattcaat
      361 tggttactat cttattgtaa atgttgctat taagatggtg atcctaccca aaaaataaac
      421 caggaattta aatctcctgg tttatttttt atctttttag aaaatttgtt gggtcgaaat
      481 ttttagaagt aaatgtttga ttggtttctt tttgacgatt ttttggttga tttcgtttgg
      541 agggattaag ttgtttttca attttttctt tcgtttcttg atacacttta gaatttgtgc
      601 caataagaat acctgtatct tttagatata aatcagcaaa ttctgcaatg atttcggaca
      661 tgatggaaat ttcttttccc atagcattta gtttggcttg gttgctggta gtcgtagtat
      721 tggtatcagg ttcataactc atacataaaa aacgaatggc ttccgagtaa ttgttgatat
      781 ctggatttct ttcgataatg gaatctaaaa cgtccacgtg actgggataa agggtaacgt
      841 gtttttgaac agaagttttt ctcatgttat cttcctccga taagtgtttc aatgagttgg
      901 ttgtttgtac gaacatattc attcagcgtt tcagtatttt tttcttgaat agcgataaat
      961 ttttcgagta ataaatcgaa ttgatctttc ttgtaatgca taagtgcgtg gtcaaaatcg
     1021 ttttcgatta ggacttttaa gtagcgattt cgactccatt ttttgccagt tttatttgtg
     1081 agttcttggc aacgtttgtc tatttttgca ataacctttg aatcgatctt ccgaactaaa
     1141 atgtccatag aaaaactcct ttcgcatttg tgtataattt atgtatatca tacgaatata
     1201 gtatttgtat agctgttttt tgctaaaata ctatacataa gctagccacg atggcgaaaa
     1261 agtgatatca aatttttttc tgagaaacgt gataggcaca gcctatctac ccactttttt
     1321 tagaaagtgg gtacacgttt ccttatgctc tttataaaat ggtgatgtgc gacttttttc
     1381 tgttcgtaaa aataaaaaaa gtttacgaaa atgaaactaa actgaacgat tatgacgggc
     1441 atgtaatcaa gatgaaatga ttttataagc gcgtattttg cgttctaaga cgttttagcg
     1501 ttcatttcgt ttagttatcg gcacaatcgt taaaacacgt aagagaagcg agaatggagc
     1561 ctgtaaggcg actggtagcg aaacggcgtg ttagattgtg caagccgaga actgaatgaa
     1621 atgaagagcg gaagcgtgaa ataatttcgg caggaggagg ctcaaggggt tcggggaatg
     1681 aaattccctg aggtcttttt atttttttaa gaggagtgct ataatcaaag aaaacgagaa
     1741 aaaagagcaa aataaaagcc gaacggtgtt ggaagcaccc ttcggctaat tacggaaact
     1801 actcacctag ttccgtaact caaattcatt atacgaatat ttttaggttt cttcaagcta
     1861 gagatttttg gatatggatt tttagagtta cgtgaactct ggaaattctt tccaaaaatc
     1921 tcttttttct ttgaggagga acaaaatttg agtaaacgtg gcacgatcat aaatgagtgg
     1981 gaaaaactta accgcatatc taacaggcgg ttaaatgcac aaactatgtg caccccctta
     2041 aaaatgcaat ggtcaattga ccgaattacc attgttggta cattaaggga aaatatttat
     2101 tatcacactc cccatgatgt gttgatttta gattttgaac agcttatgcg tctaaacgaa
     2161 ggtaacggat atttaaaagc ggttggaaat aatggctggc aattactaga ccaacatgag
     2221 gaaaatatag cttatattga gattcttaag tttcaagagg gaaaagggcg gattgacttt
     2281 aaccccaata aaattagcca atttctggcg ggctcgatga agaactttat ccatgattta
     2341 tttttagaac cacatttctc acgtgcagat attgcttgtg acattattga cgtgccagat
     2401 gatttcatta cccaatatcg ggtggttgat cctgtttctt tcaaaccaat ttacggtcgg
     2461 tctgggaaac tggaaacagc atattgggga agtcgtgcta gtgaacgaca gattcggatg
     2521 tataacaaaa aattggaaca agaaagaaaa cggaaagttg ttccgaaaga aatcgatact
     2581 tggtggcgac tagaattgca attgcgaagg gggaaagcaa ctgattggca tgcaatggtt
     2641 catgaaagtt tagatagctt tgcaagtcct cactatttgc cagaagatgt gaaagtaact
     2701 gacagaatga tgattactgc tcttataacg gaacatagtt actgggggca aattcataga
     2761 aattcaaaat ataaatatcg tgaattgtta aagcaagaaa gtcaaaatga tgagttgacg
     2821 aatcatttgc gagaaacatt ttcggagtcg gcagatgatt tgaaacgaga attagataca
     2881 tggcttcaag gacttgatgt gacggaagta ggtgctgaat gagtgattcg atagaaaaaa
     2941 tgaccct
//
LOCUS       DAZUMK010000195         2889 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000195 DAZUMK010000000
VERSION     DAZUMK010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.202"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            563..>2889
                     /gene="esp"
                     /locus_tag="ACG808_002810"
     CDS             563..>2889
                     /gene="esp"
                     /locus_tag="ACG808_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFS5048382.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT         1048 a          441 c          567 g          833 t
ORIGIN      
        1 aaagaatcaa taatttaatg aggctgtgac aaatgtcatt ttttgtttcg ctaaaaacga
       61 gtgaaaaaat gttgataact ccaatcattc gtttaagatt ctaggaatca ttcagaaatg
      121 ctgtgaaacc gatactttat aattataaag aaggtgattt caaattccga agaaggaatt
      181 tgaagtcacc tttttactta tgtcacagtc tcattcagta atgcgggata aggctcctta
      241 ttccgttatt tgaatactct gaagtttaat ctaagaaagg gtaatatttt gaaaacatgc
      301 ttcatcgcat agaaatgaac ttaatttcag aaagaataca tacatatctt tttatatgtt
      361 ttcagctgct gaatggttaa aaagcacaaa tatccgaaaa aaacatgaag aaatgtactt
      421 tttttgttgt aatgttcatg aaattctcta agtaaattag ttaacataag gttagtaaga
      481 ttcctattct gtgaataata cgtatcagat ttatctttta ttcttactag gaaagtaatt
      541 attaagggag ttgatttgaa taatggttag caagaataat aagagagtat ttctagaaaa
      601 aacaaagaaa cgagtattga agtacagtat caaaaaatta agtgttgggg ttgcatctgt
      661 tttgattggt gttggtcttg tcttgggaac aactgaacta gttaaagcac aagatgaaat
      721 ttctccaagc agcccacttg aaacagcgat aagctccgta caagtaggtg acaaagtagc
      781 ttcggggaat acttttcagg agaatcctgg ttatacgaaa aattataact tttcggattt
      841 acaatttagt cctcaagaac taactggaga cacactaaaa ggcaatacaa ttggatttga
      901 agtttacgga aaacataata ttgcagcatc aactaaaaac tgggaaatcc gtcttcaatt
      961 agatgaacga ttagctaaat atgtagaaaa aattcaggtc gatccaaaga aaggaattgg
     1021 atcttctaga cggacctttg taagaattaa tgattcgctg ggtagaccta caaatatttg
     1081 gaaggtcaat tatattcgtg caagtgatgg attatttgct ggggcagaaa caaccgatac
     1141 gcaaaccgct cctaatgggg taattacgtt tgaaaagagc ctagatgaaa tttttaaaga
     1201 gataggcata gacaatctca agaccgatcg tttaatgtat cgaatctact tggtaagtca
     1261 tcaagacgat gataaaattg taccgggtat tgatagtacg ggctactttt taacagattc
     1321 agatgatttc tataatagct tagatgtatc tgaaaataat cctgaccagt ttaaacatgg
     1381 atcagtaagt gcaaaatatg agaaacctaa tactcaaaca aaagatggtt caggatccac
     1441 aggtgctaat ggagctataa tcttagatca taagttaacg aaaaattata atttctcata
     1501 ttcatcttcc gcaaagggca cgccttggta tgctaattac aaaattgatg aacgattagt
     1561 tccatatgta gcgggtatac agatgcacat ggttcaggct gacaaagtaa catatgatgt
     1621 ttcgtttgaa tccggaacaa aagtagctga tttagcaatc gagcgacggg aaggtcatga
     1681 aaattatggc ataggttcaa taactaataa tgatttaact aaacttatcg attttgctaa
     1741 tgcaagtcca cgtccagtcg ttattcgata tgttttacaa ttgactaagc cattagatga
     1801 aatcttagaa gatatgaaag caacagcaca agttgaagaa aataaaccat ttggtgaaga
     1861 tttcatcttt gattcttggt tgtcggatac gaataaaaaa ttaatccaga acacttatgg
     1921 aacaggttat tattatttgc aagatattga tggtgatgga aaccctgacg ataaagaaga
     1981 gagcggagac acgaatccat atatcgggaa acctgaatta gaagaagtat atgatgttga
     2041 cacaacagtt aaggggaaag tattcatcca cgagttagcg ggaacaggtc acaaagccca
     2101 acttgttgat aaagaaggta ctgtattagc agaaaaaact atcgctccaa atgaaaaaga
     2161 tggggctcca atttcagata ctgtagaatt tgaatttacg ggtgtagatt caagtaaatt
     2221 aatcgcgaaa gatgaattaa aaatccaaat cgtttctcca ggttttgata aaccagaaga
     2281 aggttcaacc gttattaagg aatcaccaaa agcggttgat aaacaaaccg tggtagttgg
     2341 atttaaacca gatgctaaag aatcaattcg gaataataaa aacttacctg aagatgcaga
     2401 gtattcatgg aaaacagagc ctgatacttc taacgttact gatagtacga aaggtattgt
     2461 aactgttaag atcggaaatc gaactttcga cgtggatgta gagtttgctg taaaagcttc
     2521 tcaagctatg gaaaatgatg caacatacgt acctataaca acaactccag aaacgacagt
     2581 tcaaagtgga aaacctacat ttgataaacc agatgttcct ttagctaaag atgccttttc
     2641 aatttcagat gtctataata aggacttcgg taatgcaagt gttgatgcaa acactggtat
     2701 tgttacattc actccagcta aaggtgtagg agaatcggag ccgattactg gaacaattcc
     2761 tattaaaatt gtttaccaag atggttctgt aggcacgacc gatttagcag taactgtaag
     2821 taaagatatt tatgaaaatc caggagaaaa cattcctgca ggctaccaca aagtaacctt
     2881 caccgcagg
//
LOCUS       DAZUMK010000196         2751 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000196 DAZUMK010000000
VERSION     DAZUMK010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.203"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      102..276
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            414..2006
                     /locus_tag="ACG808_002811"
     CDS             414..2006
                     /locus_tag="ACG808_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS5048383.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            2019..>2751
                     /locus_tag="ACG808_002812"
     CDS             2019..>2751
                     /locus_tag="ACG808_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS5048384.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRIL"
BASE COUNT          866 a          517 c          603 g          765 t
ORIGIN      
        1 ttatcagtat tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta
       61 aacttttttt gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg
      121 agaatcatta accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc
      181 gaaatgtgca cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact
      241 gtcttagcag tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat
      301 tttagcggcc tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta
      361 ccgcatttca tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa
      421 aaaatatggt ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat
      481 ctctggcaaa tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg
      541 ggtatgcacc cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag
      601 gccaaaatag ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt
      661 tagggaaaaa attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat
      721 caggcaagat cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa
      781 ttgattttac cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca
      841 aatatgcaaa agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa
      901 atacattcca ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg
      961 ataatttctc agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg
     1021 aacgtccgga aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca
     1081 gcggtgcaga tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta
     1141 aaggcgaccc ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac
     1201 gaaccaaaat tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa
     1261 aacctggcat catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac
     1321 gaggtgctgg acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg
     1381 gattactgat tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct
     1441 tccaaaaaat cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga
     1501 tgatggtaca agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga
     1561 accgaacgat tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga
     1621 tcgttcgtgg ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg
     1681 gtatgaccca tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct
     1741 tgccagcaac aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa
     1801 tcggcgtagc tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct
     1861 tccaaacatt tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa
     1921 tcctgcgggc ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa
     1981 tcgacaattc aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa
     2041 tcaaacatct aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag
     2101 tcaataaagg tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct
     2161 tgcgctgtat caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa
     2221 atgtattagc tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc
     2281 aatccttcaa cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa
     2341 ctgtcctgaa acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag
     2401 ttggtatgga tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc
     2461 gtgtcgcaat tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta
     2521 cttcggcgtt agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac
     2581 atactggctt gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg
     2641 accgtgtcat ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct
     2701 ttgtccatcc gaaagaagaa cgtacaaaag aattcctttc acgtatttta a
//
LOCUS       DAZUMK010000197         2748 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000197 DAZUMK010000000
VERSION     DAZUMK010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.204"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(76..1689)
                     /locus_tag="ACG808_002813"
     CDS             complement(76..1689)
                     /locus_tag="ACG808_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS5048385.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(1830..2738)
                     /locus_tag="ACG808_002814"
     CDS             complement(1830..2738)
                     /locus_tag="ACG808_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5048386.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
BASE COUNT          848 a          485 c          496 g          919 t
ORIGIN      
        1 cctatatact ttttcatttt tactcctcaa gtaaaaataa ctcctagacc gcggctaccc
       61 accgcggtcg tttttttatt cttcaatcaa aatcgctttg atacccaaac ctgccaagat
      121 tcgggcagaa gtataagtat tcaatcctct tctgaaagta atgtaaactt ttttatcttt
      181 tgggatttca ttgattcgat ctcgtaactc attcattgga atattttttg ttgccgagat
      241 actgccagaa ggtgctttat taggttcacg tatatctaaa aagaacgctt ctttcaaatc
      301 gttttcgggt atatcacttg ctttgatcgt ttctacgata ttcgtcattt gattgattgc
      361 gacataacca gctatattca acgggtcacg ggtagtagaa tacggtggag agtaaggcaa
      421 ctccaaatca ggcaaatcaa agactgtcaa gtttccggtg attgctacag aaagttctcc
      481 catccgttta tcgacacctt tttctccaaa tgcttgtcca cctaaaatac gaccgctatc
      541 tgcatcaaaa atcagcttta gatttaatct tgtagcttct ggatagaaat aggcatggtc
      601 aaagggagtg ataaaaacag ttttataatt tgttatgcct gatgctttta attcatgttc
      661 tgtcattccc gcataacttg ctgtatgatc aaagattttt gcgacacctg caccaatata
      721 tccgcgatag cgcataggcg taccatttaa aatatctgcc agcatatgtc cttgacgatt
      781 ggccgcactg gaaagcatac tttggatcgg ctgaccagtc actacactag tcgtttcgat
      841 aatatcgcca atcgcataga tgtccggcag attggtttgt aactggtcat caacaatgat
      901 ctgacctgta tctgataatt gtatgccggt tgctttcacc acttcattct tcggagaaac
      961 accaacagca aaaattagca tatcagcaga aaggacggag ccatctgata atactatttc
     1021 tcgaccttta tcacggatct cagtaactct tgtttctaaa tgaacggcta atccttcttt
     1081 cagcaatttg tcataaacta aattagcaat ttctggatca taagggaaag cgacttgtgg
     1141 caattgatcg attaaagtca cgtctaagcc gcgatgcttg aaattctcag ctaattccag
     1201 acccataaca cctgcaccaa gaatgacgac atgttgtgga tttttttctt caaggaacga
     1261 tttgattcga tccgcatccg tcacactacg gaggacaaag ccgttttctg cttcaaaaac
     1321 tccgggaata tccggataat ctggtctagc accagaagag atgatcaact tatcgtaatc
     1381 tgtttttatt tcttcatttg tggataggtc tttaactgtt aatcgcttag tagaaggatc
     1441 gattgctgtt acttcgtgtt tagtaaagac atcgatgttg ttttttgttt tcaatatttc
     1501 tggtgtacgt tcgatcagcg agtcacggtc agtgatcaca cctcctaaat aataaggcaa
     1561 agcacaactt gcataagaaa tattttctcc gcgttcatag ataatgattt cgtgcgcttc
     1621 attcaatcga cggaaacgag tggcaaatga aggaccgcca gctacaccgc caatgacaac
     1681 aactttcata aaaacatctc cttttctacg ttgatcgtac tatgttatgg taaaaaacag
     1741 caaataattg tgccggaaaa ttattgttta ttttgaatag acagaaaata tagagaaata
     1801 atttgtggat aaaacctaaa aatttttttc tattttaagg atagataata ctttgaaggg
     1861 gataaccctg tatatttttt aaaagctgaa gaaaaatgat ggatcgaatg aaatcctaat
     1921 atatctgcaa tttctgtata ggttaattca ttttctagca cgagacgttt tgctttttcc
     1981 atttttagtt ctttaaaata gctaataaca ctttttccat tcacttttct aaaatgtttt
     2041 tttaagctgg ctggactgat aaaaaaaatt tgggctaatt gttcaagcaa aaaattttct
     2101 gaaagatgtt cttctaaata tcgtatgatt ggatcaacta attcatctga ataaagaata
     2161 tgcttttttt taggaatgac tttctcattt gaaggtattg tcaataaagc aagttcctgt
     2221 tgataatgcc ttggtaagga agacaattga attttttgca gacaataagt aactaataag
     2281 gaaagcaaat gaatgctcat ctgttggcat agtatgaaaa aagaattgat ttcctgatca
     2341 taggaaatag ctggcttact atgttttaga taatcaattt tgaactgttc atttttcaga
     2401 aaagtaatga tagatgttat ttcttgatct atagaaaaag attgtcccgt aatatgtgct
     2461 aataattgct catcaaaata aaactggata atgataaaag tttgtgtttc cttttgagta
     2521 gaaaagattt ggacttcttg ctgtttatca atgaataaaa gggaaggatt atctagttct
     2581 atagatttat gtgcaaattc agcatataaa ttagcgcttt catttattag tagtcgaaaa
     2641 tagggagcag cagataaatg aagatgggta tctatggaat aggttagtgt ttttttgaca
     2701 agattggagg attggttgtt tgttaacaaa agaaccactt tcctttct
//
LOCUS       DAZUMK010000198         2715 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000198 DAZUMK010000000
VERSION     DAZUMK010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.205"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..378
                     /locus_tag="ACG808_002815"
     CDS             <1..378
                     /locus_tag="ACG808_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048387.1"
                     /translation="IEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIVDYFANT
                     PEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKKKEYNYI
                     VKAKVSKRFIFNPKLEIYSVVIE"
     gene            703..1320
                     /locus_tag="ACG808_002816"
     CDS             703..1320
                     /locus_tag="ACG808_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5048388.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            1361..1660
                     /locus_tag="ACG808_002817"
     CDS             1361..1660
                     /locus_tag="ACG808_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFS5048389.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1694..1861
                     /locus_tag="ACG808_002818"
     CDS             1694..1861
                     /locus_tag="ACG808_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS5048390.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1906..2511)
                     /locus_tag="ACG808_002819"
     CDS             complement(1906..2511)
                     /locus_tag="ACG808_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5048391.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT          951 a          409 c          495 g          860 t
ORIGIN      
        1 atagaatgga ataatctgac tcaaaataat aaaaataatt ctggatgtgg aattatcttt
       61 atgtttgtta tagttttggg aatagtagta cttttaataa ctgtcaagat agtagattac
      121 tttgcgaata ctcctgaaga acaaatacag agaaaacttc ctgagtttga aagagtacca
      181 ccttatgatc caagagataa ttcatttcaa gtaaaaaaat taaaagagtc tccaaatgaa
      241 atgtggtttg aagttacagt tgaatcaagc gacttcaaag aaaaaaagaa agaatacaat
      301 tatattgtaa aggcaaaagt atcaaaaaga tttattttta accctaaatt agaaatatat
      361 agtgttgtaa tagaataata gtgttaaaaa aattgtaaaa gttaaagaag tcaaattctg
      421 atgtgaaaaa taaaatatac catggaagac ataaaagaaa aactggataa ctagtttatt
      481 taatggcaga ccgttaaggc ctgccatttt tatgtatttt agcggaataa atataatcat
      541 gcgggagtgt aaacttactg tttcaaaaag tatacttttt gacatgcctt gattttcttt
      601 gtttatgtat ggcataataa ccacataata caatttgaaa cgtttctgat ttaatctgat
      661 tgttttgaaa caattttagt gaaaaaaggg tagataaaaa cgatgagaaa atttttttat
      721 atccgggtca gtagttatga tcaatcgatc gaacgacaac tgatttccgc gaaggaatta
      781 ggaatccctg aagaatatat atttgtagaa aaagccagcg gcaaagattt taaacgccct
      841 gaatatcaat taatgaaacg gatgtttcga aaaggagatt tactctatct acaatcatta
      901 gatagactag gcagaaataa acaaatgatt ttagaagaat ggcgagaatt aactaaggaa
      961 aagcagattg atatttctgt gttagacatg cctctgttag atactgctag ttacaagcaa
     1021 ctagatgggt tagagacgct tatctctgac ttaattctgc aattactcag ctatatggcc
     1081 gaagacgagc ggaaacgaat taatagccgg caaagagaag gcgtagaagc tgcgaaacaa
     1141 aagggtgtaa aattcggcag aaagaaaatt gctttacctc ctgaatttcc tgcgatctat
     1201 tctgactgga aagcaggaaa gataactgca gtctatgcta tggatcaatt gggcatgaaa
     1261 cgaaatacct tttatcgacg ggtgaaagaa tatgaacata ccttgcacca cttttcttga
     1321 tagatataac agctattaga cgggaaggga gagtttgaaa atggacttaa cgaatcaaag
     1381 ggtcgttttt actggaacgc tgcagcaaat gacacgaacc caagccatcg gtttggttca
     1441 ctctttaaac ggccattgtc aatcaagtgt tacttcaaaa acaaatttcc tggtttgtgg
     1501 tcagtattct aagtcgttgt ttgatgattc tctctataca aaaaaggaac aaaccgcgcg
     1561 ggacttgatt gcgttaggtc atgagattac cattttatca gaagtcgaat tttatgcgtt
     1621 aattagtcaa cagttgaaag aatggcaacg gtatttataa tcgttagtaa aatttacaag
     1681 gaggaaaatt tttatgaagc gaaaatctta tgatcgttgg ggcattgaaa taactagttt
     1741 taatcgaaat attgcttttc acgtggagtg tgactgtggg gaattagcaa aaagtgttta
     1801 tcaaaaaaat tacttttact gtcctaaatg ccatcgccga tacggggtac atggaacata
     1861 gaaacgaaat agtgcggtag aaaagcgaat gaatgtagta tatttctaca aatcattcgc
     1921 tttctttatt ttataaatag ttggtcgaga gatcccgttt tcttttgcaa tctctgcaac
     1981 tggaatgcct tgctttaaca tgtccaccac ggttttaaaa actaagcgtt tttgcggatc
     2041 tgttgagtta atagaatact cgattggtcg tcccttatat cggccggctt tttgagccaa
     2101 taatactcct tgccgctgac gttcacgaat tttctcacgc tcgttttgtg caatataact
     2161 tagcagtgat aaaaacatat cgaacatagc ggtatccagg agttcatttc cagtattaaa
     2221 atctaaaaac tgtgcatcta atatttttaa cgctactttt ttctgtttca taaaagcaac
     2281 tgtatttttt atatcttcat aatctcttcc taaacgatct agtgatgtaa cgatgacgca
     2341 atcaccttca cggatataat ttagtaattt ttgaaactca ggacgctcag tatctttacc
     2401 actggctttt tcttggaata atttatcaat gttaagtcct tccatcattg ccaattgtcg
     2461 atccaaattt tgatcttgtg aagatactcg aatatagcca atttttccca taaaaaactc
     2521 ctttttaaaa gtgatataat ttacatgtaa aatttacttg tagttttact gctatcatag
     2581 catttttaaa aataaatttt atgtagactt aaaatttact tcatggaggg tttaaatgga
     2641 tgaaatcaaa agtaacaaaa aagcatttgg catttattta ggagaaccta gtacagaaca
     2701 attaaaccaa ttttt
//
LOCUS       DAZUMK010000199         2707 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000199 DAZUMK010000000
VERSION     DAZUMK010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.206"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            28..1188
                     /locus_tag="ACG808_002820"
     CDS             28..1188
                     /locus_tag="ACG808_002820"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFS5048392.1"
                     /translation="MAKGKHQKKQQISLSFLAVLLLAVSFFSTGIITGWQLTKQPQPQ
                     IYKTEPAQLASNLTSNEQMLASKIDQLLQKSDYIGSIYVRKNNRVILEKGYGYANLRI
                     KESNSPSLYYQIGSIQKAMTALLILKQIQSGKITFDTKLSEFYPQIPGSQQISIKNML
                     YMRSGLHRTASPKKPMSDSEIIQFALHHLKFTGYDTYHYEPLNYTLLTGVLIKLMNQP
                     YETLLKKEIIRPLHLEHTDFYENVKNSPQHAVSYQMSATDDYSKALVEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGDIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVAAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVNLKS"
     gene            complement(1228..2175)
                     /locus_tag="ACG808_002821"
     CDS             complement(1228..2175)
                     /locus_tag="ACG808_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFS5048393.1"
                     /translation="MLNYLITGGAGFIGSTLANYLGKENNVVVIDDLSMGKKENLDES
                     KHITFIEGDVADKNLMEKVMKEYHFDYIFHLAAVASVADSVERPLETHRVNFDSALLL
                     LELVRKYQSGLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFAAEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKTEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILNGMERYLKTEID"
     gene            complement(2208..>2707)
                     /locus_tag="ACG808_002822"
     CDS             complement(2208..>2707)
                     /locus_tag="ACG808_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFS5048394.1"
                     /translation="SQHINNTQTPDNTLVRKDVAENTDISNARITIWKSAWEIFKTSW
                     LIGVSPKNVVSYAQSIIPDGYIAKVGIAIHNAYLNVLTSTGILGIIPFMAFLIKSFVL
                     NVYRIIYNKCIGKDYHFVYCLVILSYALYAALNNELVYENTVGTFVFWLFLGRVNSKN
                     NSSYF"
BASE COUNT          916 a          521 c          425 g          845 t
ORIGIN      
        1 gttaaaaatg gataggaagg atttaatatg gcaaaaggaa aacatcaaaa aaaacagcag
       61 atctctcttt cttttctcgc agttttgttg ctggctgtca gcttcttttc aacaggcatc
      121 attacaggat ggcaattaac aaaacaacct caaccacaaa tttataaaac ggaacccgca
      181 caacttgctt ccaatttaac atctaatgag cagatgcttg ccagcaaaat tgatcaatta
      241 ctgcaaaaat cagattatat cggttcgatt tatgtacgca aaaataatcg agtgatatta
      301 gaaaaaggat atgggtacgc aaatctacgc ataaaagaat cgaattctcc ttctctttac
      361 tatcaaatcg gttcgatcca aaaagcgatg actgctttac ttatcctaaa acaaattcaa
      421 tctggcaaga taacttttga cacaaagcta tctgaatttt atcctcagat tcctggaagt
      481 cagcaaatct caatcaaaaa tatgctttac atgcgctctg gtcttcatcg aaccgcttct
      541 ccaaaaaaac ctatgtcaga tagtgagatc attcagtttg cccttcatca tttgaaattt
      601 actggttatg atacgtatca ttatgaacca ttaaattata cattgcttac aggggtattg
      661 atcaaactaa tgaatcagcc ttatgaaact cttttaaaaa aagaaatcat tcggccactc
      721 catctagaac atacagattt ttatgaaaat gtaaaaaatt cccctcagca tgctgtatct
      781 taccaaatgt ctgcaactga cgattattca aaagcattgg tcgagcctga aacagacatc
      841 cgaaatgaat taggcacagg aaatataagt atgtctgttt atgacttgaa caaattcttt
      901 accagtgttc tgtctggcga tattatacca aaagaactac ttttctcatt gtggcaaaat
      961 aatggtgata aacatcctta cagcggcggt gtttacagtg gaaatgatta tatccttgct
     1021 cagggaaata tcaatcgatt ccatgcagta gcggcgatga aaaaagacac aaaagatgca
     1081 atcgtcatgg aaagtaatat ccaatctgat aaaaacatca aattgccggc cactgattta
     1141 agaaatcaga tttacgaact gatggaaggt gttaatttaa aaagctaagt cgagacaaaa
     1201 aaaccagcag gatgctggtt ttttgtttca atcgatttct gtctttaaat aacgttccat
     1261 tccgttcaat atatcaaatt tgggttgata acctaaagaa cggatctttg aaatatccgc
     1321 taaagaatca cgaatatccc ctgaacgctc cgcttcatat ttaagcgcta gctctgttcc
     1381 taagatttga tcaattgaat gaatcaattc aagtagcgta gtgctttttc ctgtaccgac
     1441 attgaattgc tgtcctaaag ctttttcttc cttagcaaca agtaaaagtg cttgtataac
     1501 atcttctata taaacgaagt ctctagattg gctaccatca ccaaataaag taaattctgt
     1561 tttttctcct gccaattgtt ttttgtatcg gtccactaga atcgaaataa ctccgctata
     1621 tggagagtta ggattttgat ttggaccata aacgttaaaa aatcggacag cactagtcgg
     1681 aacatcatat aaatgacagt aatccaaaac atattgttct gctgcaaact tatcgatggc
     1741 atatggtgtt aaaggacgga tgaccgattc ttcttttttt ggcaacgttg gttcatcgcc
     1801 ataaactgca gctgatgaac taaataccaa tcgttttaaa ccagattgat attttcgtac
     1861 caattcaagc aataaaagcg cactgtcaaa atttacacga tgagtttcta gaggacgttc
     1921 tacagaatct gccacactag ctacagcagc tagatggaaa atataatcga aatggtattc
     1981 tttcatgacc ttttccatca aatttttgtc agcgacatcc ccttcaataa acgtgatgtg
     2041 cttcgattca tccagattct cttttttccc catcgataaa tcatcgatca caactacatt
     2101 gttttctttt cctagataat tcgcgagcgt cgagccaatg aagccggcgc caccggtaat
     2161 caagtaattc aacatttttt ctcctctttt caagataaaa tttattacta aaaatagcta
     2221 gaattatttt tgctattaac tcttcctaaa aatagccaaa aaacaaacgt tcctacagtg
     2281 ttttcataga ctaattcatt gttaagagct gcgtatagtg cataagataa aataactaaa
     2341 cagtaaacaa aatgataatc tttaccaatg catttattat agatgattcg ataaacattc
     2401 agcacaaaac tctttattaa aaaagccatg aaaggaatta ttcctagaat acctgtcgat
     2461 gttaatacat tcaagtatgc attatgaatg gcaattccta ctttcgcaat atatccatct
     2521 ggaataattg actgtgcata agatactaca tttttcggag agactccgat aagccaactt
     2581 gttttaaata tttcccatgc acttttccat atagtaattc tagcattaga aatatctgta
     2641 ttttcagcta cgtctttcct aacaagagta ttatcaggtg tttgagtatt attaatatgt
     2701 tgactat
//
LOCUS       DAZUMK010000200         2575 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000200 DAZUMK010000000
VERSION     DAZUMK010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.207"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            199..411
                     /locus_tag="ACG808_002823"
     CDS             199..411
                     /locus_tag="ACG808_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048395.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(689..2488)
                     /locus_tag="ACG808_002824"
     CDS             complement(689..2488)
                     /locus_tag="ACG808_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFS5048396.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT          931 a          458 c          471 g          715 t
ORIGIN      
        1 gcacgaacaa cataggaata taaacaaacg tttttgtcac aacgtctaag atcgtgttaa
       61 aaaatagtta tctaaaaagc agaaagatac tcaaaataaa aaataaaaaa atgaataaaa
      121 acgttttcaa atacaaggta atttgttatt ataaaataaa gaaaaaacaa aataaaaata
      181 aagaaaagag ggaatgaaat ggtaaaagca gaagtaggaa gatcttatat atgcaaaccg
      241 atcggagtat ctcatgaact aatcggctgt attgaacgta catatatcaa cacggctgtc
      301 gtgtatgttg aaagttatga ccaacgagac tatcaactta tcaatgaaag caagtatcgc
      361 atgctcgtaa aattctgtga tattagcgca cctactgaat tggtttctta aacagtattt
      421 ttgagaattc gcaagaactc ccttataata taaataaaga cagaagggag cgagaagata
      481 tgccaaaaag aaaaagttca gggaaaatgg gaattattct tgcagtgatc tctgcaattc
      541 ctgctatttt agctattgtt cgtgcgatga aggaatcaag agaaagttaa gcacacatta
      601 tgagcgtcaa acaaaaaacg catatacctg cggtggcagg ttatgcgttt tttgttgtat
      661 aaagactttt ttcattaacg atttttgatt agccgactcc caaaacaaaa tttagcagtt
      721 tatctgaata agtgatttct tcatctgttt gtttcacact ttcatttagt agtcccatct
      781 catcggttat gataccatcc acaccataaa acatcatgcg actcatggca tctgaatcgt
      841 tgattgtcca tacatatact tcttttccgt cttggtgggc agaattaatg aagttacggt
      901 tgatcgtact atattccatt gtcagaaaat cagcaggcgt cacagggggc ccaacaatat
      961 tgaaaggtaa gatgtagccc acataaaagt ttggttctgc ttcttttaaa tctgaaacgg
     1021 tttggtacgt caaactttgc aaaatatgat ggttctctaa gataactttg cggtatttat
     1081 ggacaaatcg ttcgacaagg tcatccgaat cttttttaga ggtcttgatc tcaataagta
     1141 atttttggtt tagcttatgt gctttttcca aataagaatc aaaagaacaa acaggggctt
     1201 gcactccgtt ttcagtaact gtaagatttg tcaattcttt caatgtaaga tcctgtggtg
     1261 ttccatttac ccctgttaag tgacgaagat ttgcatcatg catcactacg aaatgaccat
     1321 cttttgtttc ctgaacatcc atctctacat aatcaggatg atagtctctg cttgtttttt
     1381 caagtgcttc taaagtattt tgcgccccat tttgggtact cacacctctg tgagagacag
     1441 tcactggttt tgtctgaaca gctgaagtca gataatccat attgtaaaga cagacgccaa
     1501 taccgaaaaa aactgcaaaa agagtcaaac cagtgttttt cagcgcagag aaacgcaagt
     1561 tgggggcttg tgggataaac cacttaggga tctcaggcaa gaagccttcg tcatccatga
     1621 aatcaacaat gatataaaaa atccctacgg tagacatcac gatattgaat aataaaatcc
     1681 cttgtaaaag cgtcatagca aacacagcgc tgatcaatgc atagtctggg aagaattgtt
     1741 caaccatact ttgtgccaaa ataacaacga tatatcctaa agaagaaagg agtaaaatcg
     1801 taccgccaat cacaataaat tggcccgtga tggccaaaag acgcgatttg gtcaatgacc
     1861 agctttcccg aatggctgct cgaaaagaac gatctcgtag aatcatttcc ggtaaagcaa
     1921 aaatcagtcg aatcccaata tagccaagga aaatataaac gagtaaaaag gaactgacga
     1981 tgatccaacg attagcaaaa ataaaatcca taatgaaagc ggggattttt atttttgaca
     2041 gtagatcaga attaaaactt aatccgctga tcggtagaat caataaaaaa taagcaagga
     2101 aaaacaaaaa agtaatgggt ctcactttct tcagctgaag tatcgtcaag tgtaagagtt
     2161 gcttcaaaga gattggttct ttctttttaa taaaaaaaac actcatcaaa agaaaagtaa
     2221 attcgaaata aactaacagt aaaatcgtca tcaaaactac gattaacgag agtagaaccc
     2281 caggatgttg agaaaaaatt gaaggaatat tgtcataaga caaatagtca attgcccctc
     2341 ggtgtaaaat aaaacgggtc gtactgctca ataagggtaa acaaataaat aaaataaagc
     2401 catgcatcag caagacatca cggaaataag cttgtgttcc ttttaaaaag tcccaagcat
     2461 tacctaagct acgtttcaaa taattcatcc tatcacctgc tcttcacatt gtagcgcaaa
     2521 tctaatgaaa aaggaaaggg tgcagtgtgt tttgcagacc ctttcccaga tttaa
//
LOCUS       DAZUMK010000201         2454 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000201 DAZUMK010000000
VERSION     DAZUMK010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.210"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            79..489
                     /locus_tag="ACG808_002825"
     CDS             79..489
                     /locus_tag="ACG808_002825"
                     /inference="COORDINATES: protein motif:HMM:NF015567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5048397.1"
                     /translation="MYTIILASHKNLAKGMKETVEYILGPAPFIKVMTAYTDDNYDIE
                     KDTNILLDGTDKVLVITDLLGGSVNNHWMNYVYEKKLTKKITVIAGMTLSLIMELSMN
                     IEDYKLREKISMIIAESQKSIINCSELMEVEDND"
     gene            482..937
                     /locus_tag="ACG808_002826"
     CDS             482..937
                     /locus_tag="ACG808_002826"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5048398.1"
                     /translation="MIKLLRIDDRLIHGQVAMAWTKSVSADYIIVVDDASANDKMKQM
                     ILSLAKPVSTGLDIITSADFLSTFEKRKNQNLMIVTSSPKSAFEIVNAIPGEVDSINI
                     GGLRYAEGKEKVNEYVAITSEDKNYLAKLKESGFKLIMQATPTGKAKIY"
     gene            954..1685
                     /locus_tag="ACG808_002827"
     CDS             954..1685
                     /locus_tag="ACG808_002827"
                     /inference="COORDINATES: protein motif:HMM:NF015566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS5048399.1"
                     /translation="MIECILIGIVGYLTTIDERYFGASMMNRPIIVGPVVGLILGDLH
                     QGILIGAALEAMFIGIVTIGAALPPDVGVAGTIATALAIKSGAGADVAVTLAMPFAVL
                     AQGLNMLAFTINSFTVSQGRKAIENRKLSSFQKWHFFPLIVRLPSAVLTFLVVYFGAN
                     ATESFVNIMPEKLMTGFTVASGLLPAVGFAMLIQGMIDKKSVPYFFIGYLLAAYLGVP
                     VVGVAVAAIMVALLVIQTKRNPVNI"
     gene            1702..>2454
                     /locus_tag="ACG808_002828"
     CDS             1702..>2454
                     /locus_tag="ACG808_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017867010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS5048400.1"
                     /translation="MGDSKLTKKEINKVFWRSFALQGSESYTDMQGMGYSFSILPALN
                     KIYQGNDEKYYEAVYRNTEFFNTTPHISTFIMGMSLALEEENAKTDQFETETISSIKA
                     ALMGPVAGIGDSFYWGTFRVIAAGIGLSLAAQGNILGPILYFSIYTLLHFLGRYGCLR
                     IGYRMGANAIEKAFENNYLESFSLAASIVGLMSIGAMTSSLVRFTIPAVFKSKGGEIN
                     VQQMLDDIFPGILSLGLTFFVYFLLKKKVKVLW"
BASE COUNT          805 a          383 c          486 g          780 t
ORIGIN      
        1 tttattctta atacgaaata atttattgac atttagatta ccaatggtat attatttata
       61 aataaatgga ggtggattat gtacacaatt attcttgcaa gccacaaaaa tttagcgaaa
      121 ggaatgaaag agacagtcga atatatcctt ggccctgcac cattcataaa agtaatgact
      181 gcatacacag acgataacta tgatattgaa aaagatacga atattcttct cgatggaact
      241 gataaagtcc ttgtcatcac tgatttactt ggtggtagcg taaataatca ttggatgaat
      301 tatgtttacg aaaaaaaatt aacaaaaaaa attacagtaa ttgcaggtat gactttgtcc
      361 ttaattatgg aattaagtat gaacattgaa gattataaat tgagagaaaa aatttccatg
      421 attattgctg aaagccaaaa gagcattata aactgttcag aattaatgga ggtagaagat
      481 aatgattaaa ctattaagaa ttgacgatcg cctgatccac ggtcaagttg caatggcttg
      541 gactaagtcc gtatctgctg actacattat tgtagttgat gacgcctctg ctaatgacaa
      601 aatgaaacaa atgattttat cattagcaaa acctgtatca acgggactag atattattac
      661 ttcagcagat tttttaagta cctttgaaaa aaggaaaaat caaaatttga tgatagtcac
      721 tagttcccct aaaagcgcat ttgaaattgt aaacgctatc ccaggggaag tcgattcaat
      781 taatatcggt ggtttacggt acgccgaggg aaaagagaaa gttaacgagt atgtggctat
      841 cacatcagaa gacaaaaact acttagcaaa attaaaggag tccggcttca aattaattat
      901 gcaggctact ccaactggaa aagcgaagat ttattagaag gtggggaata agaatgattg
      961 aatgcatttt aattggcata gttgggtatt taacgacaat agatgaaaga tattttgggg
     1021 catctatgat gaatcgaccg ataatagttg gacctgttgt tggacttatc ttaggggatc
     1081 tccatcaagg catcttgata ggagcagcat tagaagcaat gtttattggc attgtaacga
     1141 ttggtgctgc actaccacca gatgtaggtg tagctggtac tattgcaacg gctctagcaa
     1201 taaaaagtgg agcaggtgct gatgtagctg ttacattggc gatgcctttc gctgtattgg
     1261 cacaaggatt aaacatgttg gcttttacga ttaactcctt taccgtttca caaggtagaa
     1321 aagcaattga aaaccgcaaa ttatcatctt ttcagaagtg gcattttttt ccattaatcg
     1381 ttcgcttacc ttccgcagta cttacatttt tagtagtata ttttggtgcc aatgctacag
     1441 aaagttttgt aaatattatg ccagaaaaat taatgaccgg tttcaccgta gcttctggtt
     1501 tactccccgc tgttggattt gccatgctaa ttcaaggaat gattgacaaa aaatcagttc
     1561 cttatttttt cattggatac cttttagcgg catatttagg agtccccgta gttggtgtag
     1621 ctgttgctgc aattatggtt gctttattag ttatccaaac taaacgcaat cctgtaaata
     1681 tttaaaggga gagtgataat tatgggagat tctaaattaa ctaaaaaaga aataaacaag
     1741 gttttctggc gttcatttgc tttacaggga tccgagtcat acactgatat gcaaggaatg
     1801 ggatatagtt tttctatatt acctgcttta aacaaaatat atcaagggaa tgatgaaaaa
     1861 tattatgaag ctgtttaccg aaatacggaa ttttttaata caacgcctca tatttcaacc
     1921 tttattatgg gtatgtcatt agcacttgaa gaagaaaatg caaaaacaga tcaatttgaa
     1981 actgaaacaa tttcatcgat aaaagctgca ttgatggggc ctgtagctgg tattggtgat
     2041 agtttttact ggggaacatt tcgcgttatt gctgctggca tcggcttatc tttagctgcc
     2101 caaggaaaca ttttaggtcc aatattgtat ttttcaattt atacgctgtt acattttctt
     2161 ggcagatatg gctgcttacg tattggatat cggatgggag caaacgccat tgaaaaagca
     2221 tttgagaaca attaccttga aagtttctcg ttagcagcat ctatcgtagg tttaatgtca
     2281 attggagcca tgacctcatc tctggtacgc tttactattc ccgccgtatt taaatcaaaa
     2341 ggtggagaaa taaatgttca gcaaatgctc gatgatattt ttccaggaat tctctcattg
     2401 gggctaacat tctttgttta ctttctatta aagaaaaaag taaaggttct ttgg
//
LOCUS       DAZUMK010000202         2445 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000202 DAZUMK010000000
VERSION     DAZUMK010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.211"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            114..1013
                     /gene="rsgA"
                     /locus_tag="ACG808_002829"
     CDS             114..1013
                     /gene="rsgA"
                     /locus_tag="ACG808_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFS5048401.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            1026..1676
                     /gene="rpe"
                     /locus_tag="ACG808_002830"
     CDS             1026..1676
                     /gene="rpe"
                     /locus_tag="ACG808_002830"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFS5048402.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            1677..2315
                     /locus_tag="ACG808_002831"
     CDS             1677..2315
                     /locus_tag="ACG808_002831"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFS5048403.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
BASE COUNT          816 a          465 c          515 g          649 t
ORIGIN      
        1 tcttttttgt gtttctatct cttttgatca attttcaagc catatacttt gagaagtttg
       61 catggaataa cacctacttt cctcttcgta tggtaaaata aacaaaaacg gaggtggcac
      121 ttatcaaagg acaaatcaga aaagcaatca gcggttttta ctatgtatac gcaaacggag
      181 agacctatca aacaagagga agaggaaatt tccggaatcg gaaaatcacg ccgcttgtcg
      241 gagatcaggt gatctttgag agtgaaaatc aaacagacgg ctatcttctg gaaatcttgc
      301 cgagaaaaaa tcaattagtt cgcccaccag tcacaaatgt tgatcttgga atcattgtca
      361 caagtatcgt agagcctgca ttttcttata atctacttga ccgctttcta gttaccttgg
      421 aatacgagtc gatcgaaccg attatttttt tgacaaagac cgacttgtca gacgacccag
      481 aaaaaatcga agagatcaag caaacctata ctaaaatagg ctatccggtg attgtaccca
      541 atcatacggg tgatacgaca gaactgatcc gttacttccc agaacggcta tcggtcttta
      601 tgggtcagtc tggagcagga aaatcgacgc tactgaatca aatatcaccg gaactgaatt
      661 tagcaacagg agagatttct gatgcacttg gaagaggacg gcatacaaca cgtcatgtgg
      721 aactgattcc tatctatggc gggttagtag cagatacacc aggatttagc tcgattgact
      781 ttttgacaat tgaagcagga gaactgccga gacaatttcc tgaatttgta gaagcttctg
      841 tccattgcaa atttagagag tgcatgcatg ctaaagaacc tggctgcgaa gtaaaacata
      901 gagtagaaac aggtggtatc gcacagacac gttacgacaa ttatttgcaa ttcttacaag
      961 aaatcgaaaa aagaaaacca atgtatgcaa aaaaagaagg taaacgaaaa taggaggaaa
     1021 caagaatgaa gatagcacca tccattttga gtgcagattt tgccaattta caaagagaca
     1081 ttgagttagt ggaaaaagga ggcgcagatt acatccacgt agatgtaatg gatggccaat
     1141 ttgtcccaaa tatcacatta gggccaaata ttgttcaggc aatccgtcct gttactaaat
     1201 taccgttaga cgtccatctg atgatcgttg atccagaaaa ctacattcca gcatttgcaa
     1261 aagctggagc agatatcatt actgtccacg tcgaagcaac tcctcatatt catcgggcac
     1321 ttcaaatgac aaaaagctta ggagttaaag ctggagtagt tatcaatcca ggaacaccag
     1381 tctccatgat caaacatgtt ttgcctttgg ctgatcaagt actagtcatg actgtcaatc
     1441 ctgggttcgg cggacaaagc tttattgaag aaacagtaga aaaaattacg gaactcgctg
     1501 atctacgtag agagaacaat tggacttatg caatcgaagt agatggcgga atcgttccag
     1561 aaacagcaaa aatctgtaaa gatgcagggg cggatgtctt cgttgcagga tcatatatct
     1621 atggagccga aaatccagtc ggacaaatcg aacgattaaa agaggcatta gactaaatga
     1681 aaattttatt agctgctgga ggtaatccgc aaaactggcc gcagtctctg ctgtccaaat
     1741 acgatgccta tgtcgggata gaccgtggtg cgctgtacct gcttcaagca ggctacccag
     1801 tagatctggc agtgggtgat tttgattcat tgtcgaacga agagcagaag gaagtatttg
     1861 caaaagccaa agagtatacc cgttcccctg cagaaaaaga cgatacagat acacagttag
     1921 gtatcttaaa aacatttgaa cgttacccgc aagcggaagt cactttgatt ggagctacgg
     1981 gcggaagatt ggatcatctt ttagccaatc tctggttagg tatggaacct cgcttcaaac
     2041 cctttgccca tcagcttatt atcagagatt gtcaaaatta tttgtcttat tatttgcctg
     2101 gagagcatat agtaagacag ataccagata tgaagtattt agcttattgc tgcttgactc
     2161 ccgtctcgaa tcttacatta aaaggcagca agtatgtatt ggaaaataaa gaagtagaat
     2221 atccgacatc ttatgcaagt aacgaatttc tttcagatac cgcagaattt tctttttcag
     2281 atggtatgat tgcagttatt caaagcaaag attgatccat aaacaaataa aaaaatttgt
     2341 ctttcaggct aaaaatgaca gaatctatta tttgctaaaa aaaagcttaa gaggagcaac
     2401 ttaccttttg tttcacggca tttagtgata aaattgaaca caaga
//
LOCUS       DAZUMK010000203         2440 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000203 DAZUMK010000000
VERSION     DAZUMK010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.212"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            11..493
                     /locus_tag="ACG808_002832"
     CDS             11..493
                     /locus_tag="ACG808_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFS5048404.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            530..1540
                     /locus_tag="ACG808_002833"
     CDS             530..1540
                     /locus_tag="ACG808_002833"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFS5048405.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(1655..2368)
                     /locus_tag="ACG808_002834"
     CDS             complement(1655..2368)
                     /locus_tag="ACG808_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048406.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT          825 a          403 c          498 g          714 t
ORIGIN      
        1 agacataaaa atgattgact tggcaaagaa attcttacac gaaaatatat attttgagtt
       61 gtatcatcat cgagccattt atacaaatga agatgctttg gtaataaaga aagaacaagg
      121 atttacaggt acagagacca aaagcttatt cttaaaagat aaaaaagata ataactacgt
      181 atatctgact tttactacga aacaaacgga tttcaaagca ttagggaaga tcgtcggtaa
      241 aaggctttct attgtttcat cagaacgcat ggaagaacaa acgggacaaa agcagggcgc
      301 tgtttctccg tttggttatg aaaaacagat tccgattatt attgatgaag agctcttatc
      361 aaatgaaaaa cttgtttttg caccaggtag accagatcaa acgatggtca tccaaacggt
      421 tgacttaaac aaaatcatcc agctacttga gatgccgata tttttgatgc cggcaaacga
      481 aaaaaacaat taaccaagta aaatagtgca tacttggagg tgaagcaaca tgataagtta
      541 tccattatta gaaagaaatg caacgattgg ggttactgca ccatcctcag gcgtatcaga
      601 ggaacaccat tcgttactcg aatcagcttt aaagagaatg gaagaaaaat ctttccaagt
      661 agttactgga aagacgcttt ggacgcaaga taaggaaaaa tcaggtccag caagaaaacg
      721 agcagatgaa ttcaatcaaa tgatgcaaga tgaaaatatc caaatgatct ttccgccttg
      781 gggaggagaa ttattgattg agattctcga ttttgtcgat tttgatgctc tacaaccaaa
      841 atggatagta ggttattcag atattagtac gttgctactt gctgtcacat taaaaacagg
      901 gatagccact gcacatggaa ccaaccttat tgatttaaga ggagagtact tggatgaaac
      961 gacagcaatg tgggaaagtg tactgaaaac aagagaagga gaatcgattc tacagcaacc
     1021 ttctgaaaag tatcaaaaag agtggcccca tgagcattca tcatcttctg ttttccatct
     1081 cactgaacca acaaattggc aatcagtcag cggagatgca gtaaggatga agggacgatt
     1141 attaggtggc tgtattgata cgatcagtca tttaatcggt accccttatg gagatgtcca
     1201 atcttttaaa agaaagtata atgatggaaa gccgatcttg tggtttctag aaaattgtga
     1261 gctatcgtca gttgatttaa gaagatcact tgttcagatg aaactggcag gatggtttga
     1321 tggcagctcg gggatcatgt tcggtagaag tgcagcagag gatttgttag attataaaat
     1381 gaaggatgta tatgaagatc tgtcaaagga attaaaacta ccgatcttgt atgatattga
     1441 ttgtggtcat gtaccgccac aactgacatt gatcaatggt gcctatgcag aagtagcggt
     1501 agaagataga aaagcgactg ttttgcaaac gtttatttaa aaaatttttt cctgccttgg
     1561 agtttatcaa agaccaacaa atctaaatag aaaagaaaat gaaagaagct gacagtattt
     1621 tgccagcttc tttttgtatg atctattaga aattttaatt atccataact atcacattga
     1681 tcagctgttt cacatcattt ggattacctg taggaatagc tactacagat gcattatcaa
     1741 taatttcttg agttgtttct ctaatgaacg ctggataaga tgtttgatct gcaggcattt
     1801 gccaatgtga aggaactgga taactagtga ttgtcccgtc atggttactt gaatacacta
     1861 aaactccctg gtaactgtat ttcccagtga gcataaccac atctttcgga tagactgcac
     1921 tccctgcatc ataaggggcg ataggtgttc ctgcaggata atgaataaca tgtagttcga
     1981 aatcacccga tgtatctcta aaagtattac ctctaattgc tagccaaaca cgtgcgtact
     2041 caatttgtag atcagaatat cctgctaacg gatctattga ctgagaagac tgtgaggaag
     2101 ttgtgtctat tgttgaagaa gaaatatttg tctcaactgt actactagat gtagaagaag
     2161 tattgggctc tgagacttta ctgcttgttg aagatgtatt cgtttcagta gaagctgatt
     2221 tctgagtact tgatttttca gctgaactgg tttgtttgat tgaacttttg tctgtggtcg
     2281 cagatggatc actattttct ttactaggag acgtacatcc tacaaggaat aaagagacca
     2341 acaataaaat aaaacctaac tttttcatat aaaatacctc attatttttt taataccagt
     2401 atagcataat cctgaatttt tgaatccgta aaaccttgtc
//
LOCUS       DAZUMK010000204         2388 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000204 DAZUMK010000000
VERSION     DAZUMK010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.213"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            197..2059
                     /locus_tag="ACG808_002835"
     CDS             197..2059
                     /locus_tag="ACG808_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS5048407.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2059..>2388
                     /locus_tag="ACG808_002836"
     CDS             2059..>2388
                     /locus_tag="ACG808_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS5048408.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          859 a          353 c          465 g          711 t
ORIGIN      
        1 aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt ttaacagtac
       61 atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct ataagttgca
      121 cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta tattagcaat
      181 gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt tgatttactt
      241 ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa atgttagtgt
      301 tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc ggaagcaagt
      361 cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg atcgaatcaa
      421 attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc aaagtctccg
      481 aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg gaatgaaaat
      541 ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg tgctggaaaa
      601 taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt tttggatcat
      661 aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag agttcacgga
      721 ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg aatactttga
      781 aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa tcattcgaat
      841 gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat tactaaatat
      901 tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga ttggtcgtta
      961 tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg tcatgcagca
     1021 ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata tctatagtga
     1081 tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg tgatttataa
     1141 agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga cgaatctgta
     1201 tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat ataatccttt
     1261 taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc aagcagttcg
     1321 acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat atattgcctt
     1381 acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag ccgtctacat
     1441 ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg aagaattaaa
     1501 taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga ttgctaccaa
     1561 agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc ctttcatcaa
     1621 agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa taaaaaaaat
     1681 acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa cgtccatcaa
     1741 taaagatact ttagaagact ttagcaagga attgattttg aaagcttata cgatttatga
     1801 acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag cttttttgct
     1861 tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg cagaaggtcg
     1921 tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg aagcagtttt
     1981 tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct catatataaa
     2041 agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat cgaggaaagt
     2101 caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt agtagaacat
     2161 gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa tgagatgctg
     2221 aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt caaagaagta
     2281 tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa tttaaccgat
     2341 gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DAZUMK010000205         2384 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000205 DAZUMK010000000
VERSION     DAZUMK010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.214"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(12..1820)
                     /gene="uidA"
                     /locus_tag="ACG808_002837"
     CDS             complement(12..1820)
                     /gene="uidA"
                     /locus_tag="ACG808_002837"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFS5048409.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(1843..>2384)
                     /locus_tag="ACG808_002838"
     CDS             complement(1843..>2384)
                     /locus_tag="ACG808_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HFS5048410.1"
                     /translation="YKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFEVEK
                     YISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIVWPA
                     SKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLMGLS
                     EFKGVSPIDAYCGLKDTFI"
BASE COUNT          733 a          488 c          293 g          870 t
ORIGIN      
        1 aaaaatccaa attatttttt ataactgaaa ttgggtattg atgaccatcg tttctgtaga
       61 tagtaagcaa tcattttagg ttttctatct ctagtaaaga ttcctttctt atttccttgc
      121 actcttaaaa tcccttgact tgttgcaaaa tctgcaaaat tccacacttg ttcaccaaca
      181 aaattttcac atgtatctat tacttcgtga tttgcttcgt aataagccac ttgatactct
      241 tctgtaaaca ttgttggtac tgtatcatga aatccagcta ctgtatctgc tccatattct
      301 gtaaacatta ttggcgtatc aggacatctt tccatccatc catctagctc ttttttcaac
      361 agatattttg catgtgccag atcccctccc gaaacatacc aaccataata acgatttaaa
      421 cataaaacat cgacaatatc tcctacctta caaacatctg gcgttgattt tagataagta
      481 acgattgtta caggtctttt ctgcggatca agttctttag ttaattcaac taaaggttca
      541 aaatattctt tagcaccttc ggaatccgaa tctggttcat ttgcaataga ccacattaca
      601 acacttgggt ggttcttgtc gcgttctatc aattccttga tcaccgtttt atgagaattt
      661 tctgttcgta tctctttcca cgtattatgt ttttccacat tatcatctaa taaagtggcc
      721 atgaaattta aatgtaaccc tactgcaggt acttcatcaa taactacaat accttctcga
      781 tctgctaatc gcataatttc ttctgagtaa ggataatgtg atgttctaaa agaattagca
      841 cctatccatt tcattaaatt aaaatcttta atatttacag cctcattcaa accacgtcca
      901 ttaacatacg aatcctcatg cttaccaaat cctttaaaat aaaaaggttt attattaatc
      961 aaaaatttgc cattggtaac ctgaacactg cgaataccaa attcttctgt ataggtatcg
     1021 atcactttat tatcatcaat taaactaatt ttcatactat ataaataagc gtctagaggt
     1081 tcccaaaaaa ttggattctt aagctgaatc attcctttct ttccttcagc ttcagctaca
     1141 agttgagcct cttcgtcata tatcgctatt aagatttttc cggtccattc tgtatcgata
     1201 ttgaattcta cacttgcatc caaatcagtt attcttgtat taatagtaat atcacttata
     1261 tagtctttcg gagttgtata taacttaaca ggtctatgaa tacctgcata attgaagaaa
     1321 tcaaaattaa ctaagttttc cttttttata tccccatttt cctgtactgt ttccttcata
     1381 atccccacag gtaaagtact atcgtcaata atattgttta cagcaactgt aattcgatta
     1441 ttcccgtctt ttacaaaccg attaatttca gactcaaatg gtgtaaaccc acctttgtgt
     1501 tgagtaataa actttccatt caaaaaaact tttgcttcat gtgttgcaga tccaaaacga
     1561 agaactaatc tttcttctaa taatctttta ggaagagtca cttctttttc ataccacacc
     1621 catccaacat gatttctaat gttctctgtt tcaaataaat cattataaga agagggaact
     1681 gaaatcggat aactctcttt ttcatttatt ggttctaaag caagttcttc ctgtaagtca
     1741 tcatttgtat ctaatttaaa tctccagaga ccgcttaagt caattacttg cctcgtctct
     1801 gtcataatgg gatacaacat ataaaccact cctatctaaa tattaaataa atgtatcctt
     1861 taaaccacaa taagcatcta ttggacttac tcccttaaat tcagaaagac ccattagttt
     1921 gtccaccaat atttctaaag tttcttgttt tccatcataa gtattaatat aagtctttac
     1981 ttgaggtaca tcagccaaat gaaacggaca ctgtacagat acaaaaattg ttggaatttc
     2041 atgaatgtaa aacggtatat ccggagttcc tttacttgct ggccagacaa ttcgttgaac
     2101 tgtgcctgga ttaacattag ctatattaat gatcaagtca tattttttag ttaaattttt
     2161 aattggtctc ttagcagcat atattccagc tatattttta attttttctt cttgcgataa
     2221 tcctttcaat tcttcttcag gtgaaatgta tttttcaact tcaaatcctt ttgctgacaa
     2281 aatctgtttc aataaatcaa tggcttgagg tctatttcca gcaactactt caccaaaccc
     2341 accttttacc ccatgtacat ctactagtaa aatcctttta tatt
//
LOCUS       DAZUMK010000206         2377 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000206 DAZUMK010000000
VERSION     DAZUMK010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.215"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            495..1526
                     /locus_tag="ACG808_002839"
     CDS             495..1526
                     /locus_tag="ACG808_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5048411.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            1635..2060
                     /locus_tag="ACG808_002840"
                     /pseudo
     CDS             1635..2060
                     /locus_tag="ACG808_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
     gene            2173..>2377
                     /locus_tag="ACG808_002841"
     CDS             2173..>2377
                     /locus_tag="ACG808_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFS5048412.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQR"
BASE COUNT          860 a          408 c          384 g          725 t
ORIGIN      
        1 tttaatttat ccaatccatc aataagcaaa caacttatat aaaaaaggat taattctcaa
       61 tataattgct cctaccatag aataaatctt catgtaaagt aatgaagtac caactatttc
      121 taatacttct ttaagaatta tcccaacaat atcgttagac aaaccattat tgaataaatg
      181 gagagccaat cgattctgaa aaaatgtttt ctaatttttc ggaaataaaa gaaagagcct
      241 cctgccatta gaacgaacca aatccatttc attgctcatg aagtaaccac atagctaaaa
      301 agaggataaa tcaattaaaa gatcaaaaga atgactgcac taataaaata actaaaaatg
      361 tatcttctta cttcatatct tttagctagt acttagttaa aaatttgctt cctaagatca
      421 agacaaactg tctatcttca tttgatgaaa tgcggtaaaa tatagaaaat gaacactatt
      481 ggagggaatt tgtaatggac agcaaaacta gaaaacaact gcaagaaaag ttaaagcaac
      541 gtacacactt gctcccttcc gtctttcaac agtttttaga agaaaatcgt tcttcccttt
      601 cattaggcag tcgctatgaa tacgcaaaag attttcatct gtttactgat tatttgagaa
      661 aagtattttc tgaagaagtg gatgatcagc tgattatcca tttagaaaaa caagattatc
      721 agaatttctt agctaaaatc tatcgacata ctcgttcgtt ccaaactaca gcgaaccaag
      781 aaacggagca attgtttgaa aatagctatt acgggatttc aaggaagcaa tattcattaa
      841 atcatttttt acgatttttg tatcaaaaag gactgattga agaagagatt tctcttttgg
      901 gtacaagttt accggaaaca accaaagcag ctccgcgata tatcgatgca gaacttttca
      961 aagattttga gcatttgttt atcccgattg aaggctttca aacaagcaga gaagctagct
     1021 ttgaagaaaa gaatagaccg aggaaccttg caattactgc cattcttctg tattgcggat
     1081 taaagatcca tgaagtagta gaacttaaac gtaaagatgt atctttgacc aagcagattc
     1141 ttaatctgaa tagaaaagaa aacagactga ataaagtacc aataccaaaa gaagcgatgc
     1201 catttctgga gaactatttt aagttaacag cacaaaatac ggacgaaaat tcttttgttt
     1261 ttcgttcctc acatgatcag caaatttctc ctcggactat tcgccaaatt ttgagcaaaa
     1321 ttacagtata taaaaacgta tcgccagaac tatgcagaaa aacatatcgt tattatgcag
     1381 aactatattt agatgatgct gacgcggtaa attatttatg tggtaatcga tacctctcat
     1441 cccattcttt tcaagatatc tgggaaaaga tggttgattt ttcttatcac gaattagcgg
     1501 cattcgttcg ggtttcttcc atataaaaca aatgtatgat ctttttttca caataaacta
     1561 tgttattata taaaggatta tgttacgatt atcttgaatt tgtatattac ttaacaaaag
     1621 ataatggagt gtgaatgatg gagaccataa cgtattcccc tttgaactta tttacgaatt
     1681 ttcaagaagc tgcagctaac ttttccgaga ccccgattat ctttgatgag ccactttcag
     1741 ctttcccaga attaggactt gaaacaactt acaaaaaatg ctgcgaaagt gtgttgaaac
     1801 gggcttatca attggctcat cttggagtga aatccggaga caaagttatt atttataaga
     1861 gttccgcttt tgacacatac cttctagctg tttctgtgtc ttaccttggt gcagtgcctg
     1921 tcatgacttc ttatcatcta ccaacaacaa caatggaagt atttatcgat cgtttagaag
     1981 accctttcat tttatttgat gatgagacaa aaaagcgagt gagagaaatt tcaaatggaa
     2041 caaaatcaaa acagattcct aacccgtggt gctagttaat tttttcctag aggaaaatgt
     2101 ctaaacatgt tggtaaatca acaaaaaaac gcatatagta gtctcaaaac tcccaagaaa
     2161 tgaggactac atatgcgttc atcaaaatat actgaacatt atacagatac ttttataaca
     2221 ttcaaggaaa ttatgagaac tgtttctaac atataccaca attgtgtacc agataagatt
     2281 aaaaaccgaa gaaatactga tcaactgaag caacgcgata cagtgattat tgcttgtgtt
     2341 atatggggca taattaatgg ctatactagc caaagag
//
LOCUS       DAZUMK010000207         2368 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000207 DAZUMK010000000
VERSION     DAZUMK010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.216"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..2144
                     /locus_tag="ACG808_002842"
     CDS             <1..2144
                     /locus_tag="ACG808_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFS5048413.1"
                     /translation="FMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFFIIFT
                     LFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKANQAI
                     TYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKRLSAK
                     EKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGVTDQE
                     LAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLAKGYS
                     RNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSMNAEF
                     QSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEITENA
                     LGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRSGQVA
                     LDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFGLGTT
                     TGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQYAST
                     VANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGFHQVV
                     HGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEIAISV
                     VLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          807 a          427 c          525 g          609 t
ORIGIN      
        1 attttatgaa aaaaatatta aatacttctt ggttagaaaa aataagaaaa ggtgcacaag
       61 aacctaagaa caaaacgttt cgccggtccc atgttccttt tcgcttaaat tttctttttt
      121 tcattatatt tacgttattt gttgcgttga ttgctagatt gggttatttg cagatcgtga
      181 acggcgaaga gatggaggcg cgagtgaagt ctacttctac gattacggtc caagaaagta
      241 ctcctcgcgg aatgatctac gactccacag gaaaagcttt agttacgaat aaagcgaatc
      301 aggccatcac atatacgaga ggaacacaag ttacggctaa agatttgctt gaaatcgcaa
      361 cgaagctgaa ctcgctgatc gatgttccgg tagatgaaaa tctgacagat agagataaaa
      421 aagatttttg gctggctgat cccaaacatt tgaaagaagc cacaaaaaga ctttcggcaa
      481 aagaaaagca attagggaac agcgaacaat atagtgcgat ggtcaataaa gtcacagctg
      541 atgagattaa tttaaacgag gagcaattaa aaattgctac catctttaaa cggatgaata
      601 gtgcatatga attaaacact gtctatatca aaaactctgg tgttactgat caagagttag
      661 ctgttgtggc agaacgcgct tctgaacttc caggagtttc gactggcaca gactggacac
      721 gagaatataa tgcagcaagt tcactgaaaa gtattcttgg cagtgtgacg actgaaaagc
      781 aaggacttcc agcagatgaa gcagagaaat acctagcaaa aggttattct cggaatgacc
      841 gtgtaggaca aagttatttg gaaaaacaat atgaagatgt cctgcaagga accaaaaccc
      901 aatatgaagt ttcccttgat aatgaaggaa atgtttcgaa tcaaaaagaa atcttctctg
      961 gggaaaaagg ttctaattta atgttatcta tgaatgcaga attccaatca aaagtagaag
     1021 aaattttgaa aaggaattat caaacattga tcaacaacgg caaagcacag tattcgcctg
     1081 gtgcttatgc agtggctatg aatcctcaga caggagaagt gctagcaatg actggtttca
     1141 gtcatgaaca aggttccaaa gaaatcaccg agaatgcttt aggcacgatt actagcgcct
     1201 ttgatccagg gtctgtagtc aaggctggta ctttgacagc aggctgggca agtaacgcga
     1261 tcagcggaaa tcaagttttg attgatgagc cgattcgtct gcaaggtgct tcagaaaaaa
     1321 gctctgtctt taatcgaagc ggacaagttg ctcttgatgc agtcaaagca tcggaattgt
     1381 cttctaatac gtatatgatc aaagtggcgt taaagatgtt aggactagac tatacacctg
     1441 gaatgggact acctagttta gatgaagaag ctaaagctta tcagcaattg agagactctt
     1501 tcaaagagtt tggtcttggt acaacgactg ggatcgattt gccgaatgaa tctcctggga
     1561 tctcacgttc tgttgattat atgaagaagt tcaatgcgga taatggcaaa gaatggtatg
     1621 ctccaggtaa ctttaccgat ttggcatttg gacagtttga cacatataca ccaatccaat
     1681 tagctcaata tgcttcaaca gtcgctaatg gcgggaaacg agtacagccg cacctagtca
     1741 aagcgatcta tggcaatgac gaaaatggaa acttgggtga agtgaagaaa gaaattggca
     1801 caacagtaga aaatacggtc aatatttctg cagaaaatat ggcgatcctc cgtgaaggat
     1861 tccaccaagt tgtccatggt accgatcctt acacaacagc caaaccatta gccagtgcga
     1921 aaatggacct ttccgcaaaa acagggactg cagaaacggt tgcggaaggt catccagata
     1981 ttgcgacagt caatagtaat atcgtggcat acggaccaac agacaatccg gaaattgcca
     2041 tcagtgttgt cttgccgaac ttattagacg aacaagacca cataaacttg acgattgcta
     2101 aagaaataat ggacgcgtat tatgatatgt tcatggcaaa ataaaaaaga ggtcgtgaaa
     2161 aagccgctta gtttcgagta tgaagaaaaa ttttgtgaaa atagcttctc acatttttgc
     2221 ttaaatttga cttcataccg agaaactggc ttttgaacac cgtttactcg gcaaaaaagg
     2281 ttgtgacaaa aattggctct ttgtcaataa ggactgatga gttgtgcaaa atcaaatctg
     2341 agtcagaatg aaccacattc tggctcag
//
LOCUS       DAZUMK010000208         2365 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000208 DAZUMK010000000
VERSION     DAZUMK010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.217"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            150..1451
                     /locus_tag="ACG808_002843"
     CDS             150..1451
                     /locus_tag="ACG808_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS5048414.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            complement(1555..1755)
                     /locus_tag="ACG808_002844"
     CDS             complement(1555..1755)
                     /locus_tag="ACG808_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS5048415.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            2151..2300
                     /locus_tag="ACG808_002845"
     CDS             2151..2300
                     /locus_tag="ACG808_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048416.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
BASE COUNT          768 a          454 c          408 g          735 t
ORIGIN      
        1 cgtacaaaaa aaccgtcttt tcttggctct ataatttcta ggactgatag aaaatcattc
       61 tatcggtccc ttttgagcat gaaaaaacaa aacatacaga agctccgtta gaattgaatc
      121 accacaacac aatcaaagga gcgttctgta tgcctgatct tcatcatatc aaactactac
      181 ttggaatcaa agataaaaac attttcatca caaatgtgga atcgaagagc attaagaata
      241 tcaagagtct tgttgtttct gcgacgttaa gtaaagaaat tcgtcgctgt cctttgtgta
      301 aacagatgaa tcatgaaggc atgatcgtta agaacggaaa gaaaaaatcg ctcattcaac
      361 ttaataaatg tgcgaaccaa ctgacttatc ttgctttagc gaaacaacgc taccactgtc
      421 gtggctgcca cacctatttt acggcgaata cctacattgt cgatagaaac tgctttatcg
      481 ctaaacaagt gcgctataaa attttagaag agctaactga aaaacaagca atgacgacaa
      541 ttgctaagca ttgcggcgtt tcttggtcga ccgtttctcg gacactcgct tctctgatcc
      601 ccatgacaaa agtaaaaaga aattggttac cgcgttgttt attaatggat gaatttcgtt
      661 cactgaaaaa tcaagtcggt ccctactcct tttcttgtat ggatggcgat actggaaaat
      721 tactggatat tcttccttca cggaagaaaa aggctctcgt ttcttatttt atgcaatttg
      781 aacgacgcgc acgattgaag gtaaaggtca tcgtgacgga catgaatgtt tcttatgcct
      841 ctctgataaa ggaatgtttt ccaaaggcaa aactcgttgt ggatcgtttt cacattgtca
      901 aacatctcat tcgtaaattt gaggatattc gcgtacgaat catgaaaagc tttgatcgaa
      961 acgatccaac acaagccaaa tattatcgac aattaaaggc gttgtctcgg cttctgatca
     1021 aacgacagga caagcttgtt tatgacaaat ggaccaaatg gcgcaatttt ggttgggcgt
     1081 atctcaccga aagtgaagtg gtggatcgtc tgctttcgat ctctgacgaa ttacgaatcg
     1141 cctattttta ctatcaagaa attcttcaag cgtttcacga caaagaggcg gacactttct
     1201 ttaaacttgt aagaacgatg cccaattccg ttccaaaaga actgcattct ataaaaaaag
     1261 cttttataaa ctatgaatcc ggcattcgtt tagccctcga attgccttat tcaaatgcca
     1321 aaatagaaaa tctgcatacc cacatcaaag cattgaaacg agtcgcttat ggctttcgaa
     1381 gctttcggaa aatgaagacc agaattttct tactaaataa tctgattacc tatgaatcaa
     1441 aaaacatcta aagtagaaaa ggaattctac cttagatgtt cttatctcac tctatcagtc
     1501 ctatttgaca aagagccctt ttctttcgaa aagacggtta aaagtcaatt tttattaagc
     1561 tttaacaatg tttgttgctt gagggccacg ttgaccatct tcaacatcgt aagacactgc
     1621 ttggccttct tctaaagttt tgaagccgtc accttggata gctgaaaaat gtgcaaatac
     1681 atcatttcca tcttcaccag tgataaagcc aaaacctttg tctgcgttga accattttac
     1741 tgtaccgtta ttcataaata aattcctcct gatacgtatc aatgtacgtt ttttcgttgc
     1801 aataaatatt tgataggcaa aaggagtttt atctatgtag atatatcgct caaattacgt
     1861 ttcaaaatag attactcatt taatatatca tgctttttct aataacacaa ggataatgca
     1921 atgaaatata aaattcattg tcttctattg tcttcgaatc gaagaaaaag ttgaataacc
     1981 gcgattgatt cactttttgt tttcttgttt atttgctttt atctgcaact tttgacttta
     2041 aaatttctta actatgaaaa cctttcaatt ggtggtatac ttataaatgg aatttcgtta
     2101 tcaacgcgtt cgttgccttt cagcaagtat ttaaagaaag gagaatggct atgacattct
     2161 ataacaaaga tatacaatat cgagtaacct tggatacaga tttgaatcta tttgtcgttt
     2221 ttgacaaaga aaataccaat catgtagcga caggtgttac gatcgaacaa gcagtgcaag
     2281 aattaaaaaa atctgcataa ggagtggaaa ccctcatatg gacgattctc gttcttctct
     2341 ttaaacgcat ctactggtag ataac
//
LOCUS       DAZUMK010000209         2268 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000209 DAZUMK010000000
VERSION     DAZUMK010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.218"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..2109
                     /locus_tag="ACG808_002846"
     CDS             <1..2109
                     /locus_tag="ACG808_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFS5048417.1"
                     /translation="SSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAA
                     LKYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGT
                     PIFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLEN
                     KIYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPD
                     NAYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRT
                     MEDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINN
                     RLARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRT
                     YPDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLK
                     EVSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLE
                     EQIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSS
                     DAREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIH
                     VFSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEM
                     KRAWRKMLDDELIPLENELR"
BASE COUNT          826 a          368 c          470 g          604 t
ORIGIN      
        1 tcttccggga acgccaaagc gaatagcttg atcgttggaa cgggaagtgc caaagaatta
       61 agtgaaaccc tgttggcaga tgccaatgca aatgttgtaa tcattacaac ccgtcaaaaa
      121 ctagatgcgg cgttgaaata tgcaaagaaa caagaagaga aaaaaggaac caatcgcttc
      181 caaaaattaa tgggccaaca tatcgtgttt gtagtagatg agtgtcatcg agcgttaagt
      241 gctgaaaata tggaagaaat caaaaaaatg tttccgaaat ccacttggtt tggctttaca
      301 ggtacaccga tttttcctga gaatcggaaa caagccaaag ggcaattggc acgtacaacc
      361 catgatcagt atggagaggt cttgcatacc tataccatta agaatgcgct agaagatggc
      421 tctgtcttag gatttcaagt agagcatgaa aacaccgtgg agccgacttc actagagaat
      481 aaaatctatc ggaaattaaa agaagtagaa acatatgcag agtattcatc agaacagatc
      541 aatcgtatga ttgatcaaat ggaacctgtc aaaaaagaaa gttatttgga cccagctgtt
      601 tttgaagcag atgaacatat ccaaaaagtc attcataaaa tgttccgtcc agataatgcc
      661 tatacaaaat ttgattttcg aaatggcagg ccaacgaaat ctgcaatctt aaccacgagt
      721 tccattgaca tggccaaaaa gtattatcgg gccatcaaag agatgacgaa agagcctgat
      781 tggttaacaa aagagtttcc caatcagctc attcgtgaag gacgaacgat ggaggatcct
      841 gattttccac gaatcgctat tacctattca ttagaagaaa atagccgaga ttcaagtgcc
      901 caacaagaag aaatgcaaaa aattattgag gaatataatc agtattatga tacttcctgg
      961 tcattagcag acattgagcg ttataacgga gacatcaata atcgtttggc acgaaaaaga
     1021 gcggaattca aacaatttgg gaaacaaatc gatttagcga ttgtagtgga tcgtctatta
     1081 acaggttttg acgcccctac gattcaaacc ctatttgtgg atcgtaatct agagtatgca
     1141 ggattgatcc aagctttttc acgtacgaat cgtacgtatc ctgataagac aaaaggattg
     1201 attgtgacgt ttcgtaagcc agcgacaatg gagaagaatg tcgaggatgc gacaaaactg
     1261 tattcaaaag caaaagaaga atcaggtttg atttatccaa gttatcagga atccaaaaaa
     1321 agattcaaac aagcatatgg taagttaaaa gaggtttcat ccatggaatc cattgatgaa
     1381 catacgccac ttgaaatccg tatcgagtac gtcaaagcat ttcaagaatt gaatcgtgct
     1441 tatgaatcgt tagtgactta tgacgattac aatgatgaca tggtgaaatc tccaactttg
     1501 aacaatcaaa ttcaactttt ggaagaacaa attggcgtgt acgagaccgt gaagggttca
     1561 ttaatagagg aagaaccaga aaaagaagga gaagattttt caacgatcga attttatagt
     1621 gaaaatacca ctaagttgta tgatattgat tcaacttata ttgatcaatt actaggaact
     1681 tatgctgcga atagttcaga tgcacgagaa gaaattgaaa aagctttggc taaattgaat
     1741 aaaacagaag gagtcaaaga agtttaccga caaattttaa atgcatttga tgtggggact
     1801 ttagatagta atgaagatat ctttgtcatt aaacgtcgct attttacaca agcgagtgat
     1861 gatttgattc atgtcttttc caatgagtgg tttgtttctg agaaagaatt acatgcttct
     1921 aatctgcaat atctaccagg tgaagatcca attcctaata tgaaagcaat tatcaacagt
     1981 aaggattacg aaggatataa agccaaacat ccagaagcga agccatttaa atacccacaa
     2041 gaaatgaagc gagcatggcg taaaatgtta gatgatgagt tgattccact tgagaatgaa
     2101 ttgaggtaaa cgagaagagt taagaaaatt gtacgattca aagatagtag taaagtggaa
     2161 gtgtagaaaa tagggataga tgtcttttta cttgttcaca ttttataacg gagtatttaa
     2221 gaaagctatt gaataatgat tcttccatct ttttcattca tttaccgg
//
LOCUS       DAZUMK010000210         2221 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000210 DAZUMK010000000
VERSION     DAZUMK010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.219"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1379
                     /locus_tag="ACG808_002847"
     CDS             <1..1379
                     /locus_tag="ACG808_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFS5048418.1"
                     /translation="GQFYVNKEVNLEKLSNLLHVCKTTLLNDINNIKKEFEEQIAYTH
                     REKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDEPDYLQLTECEFISVSKA
                     YSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRLGGFNSWELPESFFERAD
                     RFIESVTENSGRFYDKENKEILLIGFAISFLRQQVCAVTIDSKFIEEIKKRPIYNYVE
                     SAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDFQQLHQVFIDNTPEIKEL
                     VATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKFYLLNEEQTNLYKEVQTI
                     FSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYIVTNSDVHYLIYREALEA
                     VTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDAVQYENIIPISINTIDRA
                     IISAVQNK"
     gene            complement(1471..1836)
                     /locus_tag="ACG808_002848"
     CDS             complement(1471..1836)
                     /locus_tag="ACG808_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFS5048419.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1833..2198)
                     /locus_tag="ACG808_002849"
     CDS             complement(1833..2198)
                     /locus_tag="ACG808_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFS5048420.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
BASE COUNT          735 a          429 c          362 g          695 t
ORIGIN      
        1 gtgggcaatt ctatgtaaat aaagaagtca atctagaaaa gctctccaat ctcctgcatg
       61 tttgcaaaac cactttgctc aatgatatca ataacataaa aaaagaattt gaagaacaga
      121 tcgcttatac acatcgggaa aaggaccgtt acactttata tttttcagaa catatacctc
      181 gatgcaagat catgcagcaa ttgtctcaaa attcgttgtt tttaaaaact tgtctgcttt
      241 atttggaaga agatgaacct gattatcttc aattaactga atgtgaattc atctctgtca
      301 gcaaagctta ttcattaaaa aaacaagtat tagcttattt taatgactgc gggatcgaaa
      361 tcgaccgcta ctcccctcga ttcactgaaa tggaaagacg tctgctgctt cttaatgtgt
      421 cgtatcgttt aggcggtttt aattcttggg agctacctga atcgtttttt gaacgtgccg
      481 atcgtttcat tgaatccgta acggaaaata gtggcagatt ttatgataaa gaaaataaag
      541 aaatcttatt gattggtttt gctatcagtt tcctacgcca gcaagtgtgt gctgtgacaa
      601 tagattcaaa atttatcgaa gaaatcaaaa aacgtcctat ttacaattat gtcgaatcag
      661 catgggaaaa cactgatttc caaacctatt ataaaaaaga agaattcgcc tttattttga
      721 cactattcaa tctttgtaat tatggtttcc attcttatca gttgattgcg gaagactttc
      781 aacagctaca ccaagtgttt attgacaata ctcccgaaat aaaagaacta gttgctacct
      841 ttgaatctca cttcaatcaa gaattattcg gaaaccaacc attcgaacgt gcgctgattc
      901 atctgatgcg ttcggcttgg gataactatc aattattcat gccagaaaaa ttttatcttc
      961 taaatgagga acagacaaat ttatataaag aagtccagac aattttttcc agctggtcca
     1021 gccagttacc ttatgatcta cggttgaatc ctaattgcat gcgggctttt gtgatcgaat
     1081 tgagcggaat tctccgtctg acaaaagaac atctgactat ctatatcgtc acaaattcgg
     1141 atgttcatta tctgatttat cgggaagcct tggaagctgt aactactttc gattttcaag
     1201 ttgcaccaac aatttattca agtatatctg atataaagaa gtatgcgcag cagtcctcaa
     1261 accgggtatt atgtgaacgt acattatata caccagatgc ggtccaatat gaaaatatta
     1321 ttcctatttc gatcaatacg atcgatcgag ctatcatttc agctgtgcaa aacaaataag
     1381 ggactgtgac aaaaatcatg tcacagcctc ttatttgttg aagatgccca tttctgtaac
     1441 aatctcttga tacacatcag gctcacaacc tcacttattt tggacatcta cgggtccgat
     1501 ctctttattt aaaaaataag aaatgatggt tcggataact accaacgccg ctagttggaa
     1561 aatatcctgc aaggtaggtt tgataatcga ttcgatgatc ccagctgaaa caagaatttc
     1621 caaacttaga agtatatagc ctcccaacat ctttttaata gcattgttct cgctgactcg
     1681 gtcgctttgc acttttgtat ggacaaataa atatttcaaa aaatccttga ttgatagaca
     1741 aacaccccaa accaataaac agatagcaaa gatatcaagt cccagtacaa gtttttcaaa
     1801 atatggaaga agaaactgca tcaactctgt cgtcatccta cctctcctct tctttgtttt
     1861 tctttgtctt ctatttcctg attcaagaaa taagaaatga ctgttcgaat gataataata
     1921 agtgcaagct tcaaaatatc tgtccaagtc ggcttgataa ctgactcgat gatatcagca
     1981 acgactagta cttccaagct taacaaaata taacttgcta gcagttttct tgtatctgca
     2041 ttacgagtat tacgttctgc atagtttttc ttgacatgag accaatcaaa aagtttgaat
     2101 agaacaagaa cgatcccgac aattaagata ccgatagaac aaatatttaa aatagcaatg
     2161 atccattcta gaaattcaat taaatttgag cgaaacatat acgctaccta ctttctttta
     2221 a
//
LOCUS       DAZUMK010000211         2206 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000211 DAZUMK010000000
VERSION     DAZUMK010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.220"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..211)
                     /locus_tag="ACG808_002850"
     CDS             complement(<1..211)
                     /locus_tag="ACG808_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048421.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIEL"
     gene            304..653
                     /locus_tag="ACG808_002851"
                     /pseudo
     CDS             304..653
                     /locus_tag="ACG808_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(925..1215)
                     /locus_tag="ACG808_002852"
     CDS             complement(925..1215)
                     /locus_tag="ACG808_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048422.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1423..2114)
                     /locus_tag="ACG808_002853"
                     /pseudo
     CDS             complement(1423..2114)
                     /locus_tag="ACG808_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          616 a          421 c          324 g          845 t
ORIGIN      
        1 atagttctat aataattctt gaactccatg tcttataatg ttctatcgaa gcacccaaat
       61 agccaagttc atctaatcta cttgcataaa ctacgtcatc cgctctcata gtcggcatat
      121 acatattaaa gagtaaaaag ataccccaaa tccctattag taacaagatc agttctttgt
      181 tattcgttat ttttctatta tgtttgttca tttttatact ccttcttata tttcctaata
      241 gagtataaca tagctttaag cattacttaa agataactta aaagttatct tttaaatact
      301 taattgttaa ataccttatt tgatgcaaaa gaaatctacg atagtcaaca aaaaaatttt
      361 cattatatcc agaaaaattt ttcttagtta aggacctatg gattgcagta atgcagaatt
      421 cgtcgaataa actgccaaag acatacaatc atattgcttt taaaattgac gaacaagata
      481 tagattcgtt tgtctcaaaa atccaaatgt taggtttaac tgtcgaacca ggacgttctc
      541 gtgtgaaagg cgaggcttct tcaatttact tttacgacta tgataatcac ctcttcgagt
      601 tacataccgg tactctccag gaacgtttaa aatcctacaa ttccactacc tgagactagt
      661 aatgactagt ctctttttat ggcctctgat ccccccattg agcgaatact aatagggctt
      721 cgaattgatc tgttcaatct ttaagcaata tccctcaaat gagtttggaa agtttagtga
      781 ttcatcacaa atacttaaat acatgtagtt atatatagat tgaggttctt gtgagcgtat
      841 gctcgtgtgg gttcaaatcc catcgtctgc atccatcttt ccaaaacaag cggctaggac
      901 atttttgtct cagccgcttg ttttctagtt tattctttca tcttgtcgga ccttttttct
      961 tgggtataga cttttctcat attctaccac aattttgggt ttccctaaca catgatagga
     1021 tctcacaaca aattgctttc tattattata ataacctgta acgactaact tcatcccttc
     1081 gtctacttca gctaggaagt tcaaactatg ggaagcaatc aaacaattga ccccatttaa
     1141 actgaaacgg atcaacggtc tggcactaac ttgcaaagta cggatcttat ctactgttcc
     1201 atattttttc tccataacct tcacctcaat accattatac gaacatacat tccctttgta
     1261 aagaagaaac tctgttttat ctttttgact ttttgtctat tacctttctt aaaatcatat
     1321 tggcacttcc tgcgaagatg aaggacatgt actaaaaaaa agaggcagca gtatagatta
     1381 ctgttgcttc tttactaatt taggtttact tgttaagaaa gattatttac gcaaatgttc
     1441 ttcaacaatt tgctgagatt tatcagctgt atcttccact tcatcttttg ctttttctaa
     1501 cttttgaata tattgtttca agcttgagtt ttcagcagtt gcttcgtcta atttcttttg
     1561 tgtatctgtt aactgatttc tacatttgtt ttttcttgtg tcagtgttgc gatttgttga
     1621 ttcaaaccat ttacagtatt ttgtaattct gaaagttttg cttgtgcttc ttgtagtctt
     1681 gctgtccaat ctgcattcag atgattgatt tcatcatttt tttggttgat ctcatctagt
     1741 ttgacattga tttcattttg tttgctttgt agttgtgcat ccttgctgac taattgactt
     1801 tctaagtttt gtttttctgc tgccgattga tttttttggt tttctaattc atttactttt
     1861 gtttggtatt cttggacatc tgcctttgaa tcttgcaatg tctgttcttt ttgtgctagt
     1921 cgagcggtaa gatctgtaat cgcttgctct ttatctgtca atgtatgatc aagctgtgtc
     1981 agattctcat tgattgtatc aatatctgtc tctccacccc acaaaataat ttcatttgcg
     2041 taccgcgtgc ctgtgtagcc aattagacat agaaatgcaa cgacaatccc accagtaata
     2101 aatttctttt tcatttgcat agctccttaa tccaatactt ttacatgcta aaagtaagtg
     2161 atctcaaaca caatgtcaag cgtttttaaa ttacatatgt aatttt
//
LOCUS       DAZUMK010000212         2204 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000212 DAZUMK010000000
VERSION     DAZUMK010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.221"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..522
                     /locus_tag="ACG808_002854"
     CDS             4..522
                     /locus_tag="ACG808_002854"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFS5048423.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            650..1333
                     /locus_tag="ACG808_002855"
     CDS             650..1333
                     /locus_tag="ACG808_002855"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFS5048424.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1642..1715
                     /locus_tag="ACG808_002856"
     tRNA            1642..1715
                     /locus_tag="ACG808_002856"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1676..1678,aa:Arg,seq:tct)
     gene            1874..>2204
                     /locus_tag="ACG808_002857"
     CDS             1874..>2204
                     /locus_tag="ACG808_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFS5048425.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLA"
BASE COUNT          734 a          346 c          447 g          677 t
ORIGIN      
        1 acgatacctg attacaaaaa aataactttt ataagttcta taacgttgat tatattttta
       61 attcttattc ttctcgtcgt cttggttccc cgaatttttg gtttgactcc ttatattatt
      121 gaggacaaag aaatgagtcc atattatgca caaggaaatc tgatatatgt acgaccgaaa
      181 aatacaaatg agattttagt cggagatatt attacatatt atgaaaattc aggaaaaaaa
      241 atctcgacgc gtcgcgtaat agccgttgaa aacaaccacg aagattttta tacaaaagct
      301 gatacaaaga cttatattga gcctggagta gtaaaaaaga gaaatattat cggtagtccc
      361 atctttcaga ttccatatat tggactagtc ctttcaaaaa cggcatttgc atggattcag
      421 gggttattct ttacgattgc tttttgccta acaggaatca cagcatggaa tacctttgta
      481 gagttgaaaa agaagcggag acgaacacag aaatttttgt aagaatgtta aaaacataaa
      541 tgtattccag agatccaacg actcttctga acatgaaaca cgagaaaaca ctcatgcagc
      601 ttgtagattt agtcagttgt tcgtataatg tgtgaagagg aggagtagta tgcgcaatga
      661 aatgatgcat cgtcctgtag tcaaagaaga actattggat tttatgcgta cccagcaaaa
      721 aaaattaccg ggtgagctag gcaaactgga agaagaagct catgtagcag aagtaccagt
      781 tattccccat gaaacagttg tatttttgaa atttttactt ggacagcttc agcctgaacg
      841 aatcttggag atcggagcag ctatcggttt ttcatcaagt gtcatggcca cgacaatcgg
      901 agaaaacgct catgttacga caattgatcg tttcgatgtc atgatcgaga aagctaaaaa
      961 aaactatgaa cgtctaggac tgacagataa agtgactttg cttgaaggtc aagcagcaga
     1021 tatcttgccg gagttgtctg gtccgtatga ctttattttt atggatagtg caaaatcaaa
     1081 atatattgaa tttttacctg aatgcttacg tttgttaaga aaaggtggcg ttttgatggt
     1141 agatgacatc ttccaaggag gaacgatctt gctcccagat gaagagattc cccgtggcaa
     1201 acgagctatc catcgtaaat taaatgaatt tcttcgtgtt gtgatggatc atcccgattt
     1261 gacatcgaca atcttgccat taggtgatgg tgtgatctta atgacaaagg aatcagaaac
     1321 aatcgagttg tgatacatga ttatggcagc ctgtagataa tcgattgaat cgagggctca
     1381 attcgaactt gatgtaaaag tttctatatt tgtaagtcaa ttaaaggata caggaaatct
     1441 aaataaacag gagtagcatg aagtcaatgt agctcctgtt ttcaattgat aaccttagaa
     1501 cagctgaagg agaatgtctc gttttaaaag attgatttag tagttgggcc aaaataattc
     1561 attatttgct aattaaataa ctatttcgta ttgacagaag caatttaatt atctataatt
     1621 attgatgttc cgctgattct tgtcatagta gctcagctgg atagagcatc cgccttctaa
     1681 gcggacggtc gggggttcga atccctcctg tgacgctaaa aaaccgttag aaactgaggt
     1741 ttctaacggt ttttctttgt tttttgtgcc aatattgttt gttttcaaag gcaaataaaa
     1801 taaaaaaaca tccttatcga gtacaattat aaggagcaaa aaaataaaac tcatcagaaa
     1861 agaggaagaa gatatgacga tactatctag aattttagtg actctagttg caattgaatt
     1921 tttttatatc atgtatatcg aaacggtgag aactgattct gacacgacaa gtagggtttt
     1981 taaaatgagt aaagaagagt taagcagaaa atctgttcaa acattgttta agaaccaagg
     2041 agtgtacaat ggactattag gagtgggact actttatgga gcttatcttt cttccgcatc
     2101 taaagaaatc acaagtatgc tattaatatc tatttttttt gttgcattat atggcagttt
     2161 gactagcgat cgttggattt tactcaaaca aggtgggcta gctc
//
LOCUS       DAZUMK010000213         2202 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000213 DAZUMK010000000
VERSION     DAZUMK010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.222"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1390)
                     /locus_tag="ACG808_002858"
     CDS             complement(<1..1390)
                     /locus_tag="ACG808_002858"
                     /inference="COORDINATES: protein motif:HMM:NF014437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5048426.1"
                     /translation="MSNKEEAKSIINFVGGKENIAALTHCVTRLRFALRDDTKVDKAG
                     LENLEDVKGTFNANGQFQVVIGPKVADIYEEVISQSGLSGANKEDVKKVAAELNLNWF
                     QRAVKILGDVFVPILPAIVTAGLLMGLNNLLTNPGIFFEQSLIEVYPNIADVADMINL
                     IANTSFQFLPALITWSTVKYFKGNPLLGIVLGLVFVHPDLMNAWNYGQAVTTGDIPHW
                     TIFGLSISKIGLQAQVLPALVSGYVLAKTELFLKKRVPETIQLLVVAPVAILFTSVIT
                     FIIIAPITIAIGNTIVAGIVWLFQKVPFIGGFAYGIGLAPLVVTGMHHAFLPVHIQLM
                     ANGGDYIWPIGVMSSLAQGSAAIAMNFLIQNKKLKGVGLAAGLSAYMGITEPAMFGVN
                     LKYKFPFVVSILTSGIIGGFMASQHVIATSVGISGIPGFLSIPFEFWPIFFICMLASC
                     VVSFGATYLYGKN"
     gene            complement(1383..1751)
                     /locus_tag="ACG808_002859"
     CDS             complement(1383..1751)
                     /locus_tag="ACG808_002859"
                     /inference="COORDINATES: protein motif:HMM:NF025668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III-like domain-contianing
                     protein"
                     /protein_id="HFS5048427.1"
                     /translation="MSYTTFSMSPISIKEKGKNCLKVTTTVSNKGSRDGYEVVQLYIG
                     AQYGRYIRPTKDLVAYKKVFVKSGDSVTVSFELTNKDFKYYDEEFNEIIDDGAYWIMV
                     GNDSSQALTNKEEIEVRINE"
     gene            complement(1755..1994)
                     /locus_tag="ACG808_002860"
                     /pseudo
     CDS             complement(1755..1994)
                     /locus_tag="ACG808_002860"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
BASE COUNT          720 a          466 c          346 g          670 t
ORIGIN      
        1 tatttttccc gtaaaggtaa gttgctccaa atgagacgac acaacttgct agcatacaaa
       61 taaagaatat tggccagaat tcaaacggaa tagacaagaa accaggaatt ccactgatac
      121 caacagaagt tgcaatgacg tgttgtgacg ccataaatcc tccaattatt ccagaggtca
      181 gaatggaaac cacgaatggg aatttatact ttagattaac cccgaacatt gctggctcag
      241 tgatacccat ataagcagac aaaccggcag ccagtccaac acctttcaac tttttatttt
      301 ggattaagaa attcattgca atagcagcag aaccttgtgc caaacttgac atgactccaa
      361 taggccaaat ataatctcca ccgttcgcca tcaattgaat atgaactggt aaaaacgcat
      421 gatgcatccc tgtcacgact aatggcgcaa gaccaatacc ataagcaaat ccgccaataa
      481 atggaacttt ttggaataac catacaatac ctgcgacaat cgtattacca atagcaattg
      541 taatcggcgc tataataata aatgttataa cactagtaaa aagaattgca actggtgcaa
      601 caaccaacag ttggattgtt tcaggcacac gctttttcaa gaatagctct gttttagcta
      661 acacatagcc ggaaactaga gctggcaata cttgtgcttg tagtcctatc ttagagatgg
      721 acaatccaaa gattgtccaa tgtggaatat ctcccgtggt tactgcttgc ccgtaattcc
      781 aggcattcat caaatctggg tgtacaaaga ctagacctaa cacaatccca agaagaggat
      841 ttcctttaaa gtattttaca gttgaccatg ttattaacgc aggcagaaat tgaaaggaag
      901 tgtttgcgat aagatttatc atatcagcga catcagcaat attaggatat acttcaatga
      961 gtgactgctc aaaaaagatt cctgggtttg ttaataaatt attcagtccc atcagtaaac
     1021 cagctgtaac gatcgctggt aaaatcggca caaatacatc acccaatatt ttaactgcac
     1081 gttgaaacca attcaaattc aactcagccg ctactttttt aacgtcttct ttgtttgccc
     1141 cactcagccc agattggctt ataacctctt catagatatc tgctacttta ggaccgataa
     1201 caacttgaaa ctgtccatta gcattgaagg tacccttcac atcttccagg ttttccaatc
     1261 ctgccttatc aactttagtg tcatctcgca aggcaaacct cagacgtgtc acacagtgag
     1321 tcagtgctgc aatattctct tttccaccaa caaagttaat gattgattta gcttcttctt
     1381 tattactcat taattcttac ctctatttct tctttattag ttaatgcttg agatgaatca
     1441 ttgcctacca tgatccaata agcaccatca tctataattt cgttgaactc ttcatcataa
     1501 tacttaaaat ctttgtttgt aagttcaaaa gaaacagtca cactatcccc tgacttaaca
     1561 aaaaccttct tgtaggctac caagtctttt gttggtctta tataacgacc atattgagct
     1621 ccaatatata gttgaacaac ctcataacca tctctacttc ctttatttga aacggtagta
     1681 gtcaccttca aacagttctt acccttctct ttaatgctaa ttggtgacat agaaaaagtg
     1741 gtataactca acccataccc aaagggaaat tgtggttcat tggatacatc taagtatttg
     1801 gtcgtattaa acttttcact tgaatacggt ctaccagtat tgaaataatt ataataaata
     1861 gggatttgtc ctaaatgtct cggaattgtt attgtaagct ttccggctgg gtttattttg
     1921 ccacaaagga cgtccaaaat agcattgcca gcctctgctc ctagatgcca agcctcaact
     1981 aatcccttac atatagattt ttaaattaca cactagttta aactaaaaca aacaaggaaa
     2041 aacttaccat ttaatcaaaa aattagctca tatgaatata ttagaatgta aattagactg
     2101 gtcaaaatct ggtcaaaaaa gaggacgaat acaggtagtc ctcttttttg ttattttgat
     2161 attctaaatc attttttatt ggattattcc cttgttcgat at
//
LOCUS       DAZUMK010000214         2163 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000214 DAZUMK010000000
VERSION     DAZUMK010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.223"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            124..972
                     /locus_tag="ACG808_002861"
                     /pseudo
     CDS             124..972
                     /locus_tag="ACG808_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1325..>2163)
                     /locus_tag="ACG808_002862"
     CDS             complement(1325..>2163)
                     /locus_tag="ACG808_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS5048428.1"
                     /translation="YQLVETQAPTGYELDATPIEFEIERSQTAVVELTKANRLTPGGV
                     VLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAP
                     TGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDA
                     NGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIF
                     LTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKR
                     NI"
BASE COUNT          606 a          456 c          372 g          729 t
ORIGIN      
        1 tcatatggcg ttgtcatttt cttgtcaact atttttccta ttcatgtttt tataaaaaaa
       61 catatcgttt tcttgtatga ttaaatcacc acaaaataaa caactgagag aacgatatgc
      121 tttatgaatg gttctatcaa aaaaattctg agatcaatag acaaaaattt gatgattaca
      181 gaggtctctt acgaaacctt acagaagaaa aaaacattga tcgttgctgc agttctcttc
      241 cctgctccat gtgtctgtag aaattgtggt tctgctgtgg tagatggaaa taggaaatta
      301 atggtggtga aaaacaagaa gaaggaaacc attgtccact ttgaataata taatcatatg
      361 ccaatggtta tacgcctaaa aaagcagcgc tatacctgta aaaactgtcg aagccattgg
      421 aacgctcaaa gttattttat ccgtccaagg cactcgattt caaatcatgt cagacataaa
      481 atcacttctt tactgactga aaaagtgtcg ttatttttta tttcgaaaag ttgttaggta
      541 tctttgacaa ccgttattcg cacattgaaa gagttgagaa gctatttacc aaagtcttcc
      601 aaaagaattc tgtcttgagt gttgagggtt gtgaatttcg ttctcacgtt tccgttgagg
      661 ataaactgag ttttatttcc gcggatggcg agacagaaaa attaatcgag aaaatttacc
      721 tacacaaaaa attcctcgat taacaaacta tttccttagc tgcactaatc ctaaagaaat
      781 agaattctta gttacagaca tgaacgccac ctactttcag ctcaccaaac atgttctacc
      841 aaatgcgaaa ttggtgattg atcgattact tggtttttcc acgcctttca aggaggcgta
      901 acctttattc acgagttggt tgaagctttc cgagacaaaa gacccgactt gtttttctcc
      961 ttattgacaa agagccatta aaactagaga aaaaattaca actgatcgct gattatttgt
     1021 agaaagaaaa tcaaagtatt acccaataaa cttgagtcat taggcaaaaa acactgcgaa
     1081 actcaagaat gattttcgca gtgtttttgt atatttttta ttaactagta ttactacaaa
     1141 ggtttatttt tcacttctca acgcagcaca aaattgtctt tacttattta ctggttgggt
     1201 tcaaccttct ctactccgat actaatattt gtttgtatac cccttctaaa atcggtagtt
     1261 tccattcaat tgatgggtac tagttgattt taaaaactat gcttattcaa ttttttattt
     1321 tagctcatat atttcgtttc tttctaaatt ttatactgat gaaaacaagt agcacaccca
     1381 atattgacaa tataaactgg ctaatgatgg tttctccagt ttttggaaga tgcttatttc
     1441 cactgcttgt tggattgttt cctcttatat ctgagctatc agcaactttt ctattctctt
     1501 ttgttaaaaa gatcgccact tcttgttctt tttcgatctt gaaagttact ggtgtcgcat
     1561 ccaattcata ccccgttggc gcttgagttt ctaccagctg atatgctcct ggtgctaagc
     1621 catctatcgc cagttttcca tctgctcctg tggttagacc tgactgcaga acggttccat
     1681 tggcatcttg taactcaaaa acagctcctt gcaggacttc tccgctttgg tcatcgatct
     1741 ttgtcaacac gacaccgcct ggtgtaagtc gattttcttt cgtcaattct accaccgctg
     1801 tctgacttct ttcgatctca aactcgattg gtgtcgcatc caattcatac cccgttggcg
     1861 cttgggtttc taccagctga tacactcctg gtgctaagcc gtcaatcgcc agttttccat
     1921 ctgctcctgt ggttagacct gactgcagaa cggttccatt ggcatcttgt aactcaaaaa
     1981 cagctccttg caggacttcc ccgctttggt catcgatctt tgtcaacacg acaccgcctg
     2041 gtgtaagtcg gtttgctttc gtcaattcta ccaccgctgt ctgacttctt tcgatctcaa
     2101 actcgatcgg tgtcgcatct aattcatacc ccgttggcgc ttgagtttct accagctgat
     2161 atg
//
LOCUS       DAZUMK010000215         2152 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000215 DAZUMK010000000
VERSION     DAZUMK010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.224"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            37..>2152
                     /gene="acm"
                     /locus_tag="ACG808_002863"
     CDS             37..>2152
                     /gene="acm"
                     /locus_tag="ACG808_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFS5048429.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLL"
BASE COUNT          846 a          343 c          418 g          545 t
ORIGIN      
        1 gttttatttt tagttaaaat aggggagata gaaaaaatga aaaagtgtag aaatattatt
       61 ttatcaatgt tatttattat tactaatata acatcactga ttcctgttca tgtctatgcg
      121 gatgcaggca gagatatcag cagtaatgtc acttcgttga ccgtggaccc aacgaatatt
      181 acagatggag gaaacataaa agtaaaattc tcgtttgacg aaaaaaaaca aaatatccaa
      241 ccaggtgatt atctttggat caattggcca agtgaaggaa atattcgcgg tgaaggattc
      301 caaaaagaaa tcccattaat gattgaaaat aaaaatgttg gaactttaac ggtcagaaaa
      361 gattctgcgc aggttgtgtt taatgaaaat attaaaaacc tagactcagt agaaggttgg
      421 gggcaatttg agatacaggc cagaaacgta accgataccg gtgaagaaaa tacgggacca
      481 ttcaccgtga cgagcggtga taaaacagct actgtaaatg tgacaaagcc agcttctggt
      541 tcttcttcta gcgtttttta ttataaaaca ggagacatgc tacctgaaga tactaagcac
      601 attcgatggt ttttgaatat taacaataac ggcacttatg ttgaacaacc agttaaaatc
      661 agtgatgaaa tccaaagtgg acaaagatta gatccaagta cgtttgaaat taatcaaata
      721 cacttaggtg aacagaaggt ttatagagga gaagagggaa tccaacaatt tttacaagat
      781 ttccccagcg ctacatttaa tttttcagta actgataatt acattgaaat aactatacca
      841 aaaaactttg tcaatcttag gaaaataatg gtgagttata agacgatcat agaaaaccca
      901 gaacaaataa attttgagaa tcattcggaa gcttggttta aagaatttaa taaacctgct
      961 gttgatggag aaagtttcaa tcatacagtt aaaaatattt cagcttctgg tggagtcaac
     1021 ggtacagtta gaggcgaatt gaagattttc aaatatatca atgacacaga gattggtatc
     1081 ccaaatgtga ctttcgaatt aagacgagca gatgaacagc ctattcaagg acagtctagt
     1141 atcctgttga cttcgaatga acagggagaa gctagtatca aaggcctgca agtaggagac
     1201 tatgttgtaa aagaaaaaga agcacccaat tggattgatt ttgatccact aagttcaaat
     1261 gaactcaaat tctccataaa cgaaaatgat acagagggag tcagtttacc tatttataac
     1321 aagaaaaagg ttacgaacat cacagcaact aaaatttgga acggtggaac cactccaaga
     1381 ccgagtattt attttaaatt attcagagca agtacaaaca attgggaacc agttcctgat
     1441 gcagaaacaa aacgattaga caatggaata acttcagtga cttgggaaga tatccaacag
     1501 tatgatgata gtggaaacga atatacgttt aaagtgcaag aagtagatca aaacggaaat
     1561 gattatgtcc cttcaggtta tcaaaaaatt gaaaacgggc tagtcgttac aaatgagaat
     1621 aaagaagtca tttcagtctc aggacaaaaa acatgggaag acaatgagaa ccaagacggc
     1681 aaacgaccga cgacgatcac agtaaatcta ttagccgacg gtcaaccgat ccaacacaaa
     1741 gaagtaagtg aaaaagatga ttggcgctat cgtttcacaa atctgccaaa atacagagac
     1801 gggcaagaaa tcatctatac tgtaacggaa gacaacgtgc ccgaatacag tacgacgatt
     1861 gacgggtata acatcaaaaa cagttataca ccggaaaaaa caaatatatc tataccaggt
     1921 agccatataa caccgtggaa aaattttcca gagaagacag agagagagaa aattagaaat
     1981 ctattctctc aaaaattgca aatatttgct catacaggag aatccagcga acaaagaagc
     2041 aattatatta gtaagagagc tgtacctaaa cagataacaa ataaatcaaa aagcattctt
     2101 cctaaaacaa gtagtaaaaa atctagtttt gctgtaatca ttggtttatt ga
//
LOCUS       DAZUMK010000216         2140 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000216 DAZUMK010000000
VERSION     DAZUMK010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.225"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..567
                     /locus_tag="ACG808_002864"
     CDS             <1..567
                     /locus_tag="ACG808_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048430.1"
                     /translation="EEQLYLESETSQTKVTVSHNRLDVYKRRPETVKKGTELLRLETE
                     KYIMNVYLERSELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATID
                     DYYLVFTRFLTCGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTY
                     TYDDTEEIQNELDRKRMPYQRFERKKRQ"
     gene            complement(614..850)
                     /locus_tag="ACG808_002865"
     CDS             complement(614..850)
                     /locus_tag="ACG808_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048431.1"
                     /translation="MKQNTSIAKNTTKFKCTAAAWRISISPTNRTNNRGMILFIWISF
                     FLLGYRVLPAYFAPPKRRGRLERLVRNKNHSLSY"
     gene            948..1127
                     /locus_tag="ACG808_002866"
     CDS             948..1127
                     /locus_tag="ACG808_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048432.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            1258..1527
                     /locus_tag="ACG808_002867"
     CDS             1258..1527
                     /locus_tag="ACG808_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS5048433.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            1520..1777
                     /locus_tag="ACG808_002868"
     CDS             1520..1777
                     /locus_tag="ACG808_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFS5048434.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
BASE COUNT          787 a          299 c          429 g          625 t
ORIGIN      
        1 gaagaacaat tgtatttgga aagtgaaacg agtcaaacga aggtcacagt cagccataat
       61 cgtttagacg tgtataaacg tcgtcctgaa acggtaaaaa aaggaacgga gcttctacga
      121 ctagaaacag aaaaatacat catgaatgtt tacctagaaa ggagtgagct ggtcgaaaac
      181 tttgtcagct acacgatttg caggaaacac tattactcag aatcagaata ttattcaatt
      241 tttgagagca tggaattaat ggaagaagaa atggaagagc taaacagcaa agatgcgacg
      301 atagacgatt attaccttgt atttactaga tttttgacgt gtggaacgga atctatcgtg
      361 ttaatgggaa tggattatga actcgctgta cccgaagcat ttttgaggga atatgtagaa
      421 gaagtagata acccaggcga cgactggtac acgactttaa ctgatttttt agacacctat
      481 acttacgacg atacagaaga aatccaaaat gaacttgacc gtaaacgaat gccatatcaa
      541 cgatttgaaa ggaaaaaaag gcaataatca tttataccga aaagtgagaa tgtgtgaaat
      601 aaggacaaaa aacctagtaa ctaagggaat ggttcttatt tcgcaccaat cgctccaacc
      661 gcccccgccg cttaggtggt gcgaaataag cgggcaacac ccgataacct aagagaaaga
      721 aggagatcca tatgaataaa atcattcctc tattgttagt cctatttgtt ggcgaaatac
      781 ttatgcgcca tgctgcagct gtgcatttaa acttcgtagt atttttggca atactggtat
      841 tttgtttgat cttttggcgc tttgtattag cccttgtgag tctactattc aagatgattg
      901 cagccatatt tttcataggg ttatttcgat gaaaggagag agtactaatg ccaattgcaa
      961 taggaaataa acggttaccc gttacgttgg atgaaaagag acaaaaagaa ttgcagcaac
     1021 taaagcagaa gtacggcaaa agtgaatcca ggattatgtg tattgcgtta gatttattga
     1081 ttgcccaaga aaaagcagga tttgaggtac cagcactcaa aaagtgacgt cactttttat
     1141 cctaaaaact aaaagtgata gcacttttaa ttataagaag ttagaatatt aatcatttgc
     1201 ttaattgtac aatataatgt acaattgttt tatagaaata aataaggggt gaaaggaatg
     1261 gaagcagtag cttattcaaa tttccgccaa aatttacgta gttatatgaa acaagttaat
     1321 gaggatgctg aaacacttat tgtaacaagt aaagatgtag aagatacagt tgttgtatta
     1381 tcaaaaagag attatgattc tatgcaagaa acgttgagaa cactttctaa taattacgtc
     1441 atggaaaaaa ttcgtcgagg agatgaacaa ttctccaaag gtgcatttaa aacacatgac
     1501 ttaatcgagg ttgaatctga tgattaaggc ttggtctgat gatgcttggg atgattatct
     1561 ttattggcat gagcaaggaa acaaaagcaa tataaaaaag attaacaagt taataaaaga
     1621 tatcgatcgt tccccctttg ctggattagg aaaacctgag ccattaaagc atgatttatc
     1681 tggaaaatgg tccagaagaa ttacagatga acatagactg atatatagag ttgaaaatga
     1741 aacgatattt atttattctg caaaagatca ctattaacca atcggaagta aggaaagggt
     1801 cagaaactta aaagtttttg atccttattt tatttaccct agtcatttaa aaagctaata
     1861 tagcttagtg ttgattgtta ttaatgaatg tgtttgttac gcgtattacg gatataaggt
     1921 tagtaaaatc atttctaaag ttgaggaaaa gtaaatataa atggcttaaa tttcaacaat
     1981 ttgaagttga atagatatgt tataatacta ttgtagtgtg ggatgttagt tactaaagga
     2041 tgacgcttat atatatgact gaatagaata agcaataggt ttaataatct attttaaatt
     2101 ttttgtacta gttttagtta attagcaaaa acaacaaaaa
//
LOCUS       DAZUMK010000217         2082 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000217 DAZUMK010000000
VERSION     DAZUMK010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.226"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..323
                     /locus_tag="ACG808_002869"
     CDS             <1..323
                     /locus_tag="ACG808_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFS5048435.1"
                     /translation="VAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPFLIIGGFLWV
                     FSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILIALSAIFMLF
                     SLLG"
     gene            complement(393..2054)
                     /locus_tag="ACG808_002870"
     CDS             complement(393..2054)
                     /locus_tag="ACG808_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS5048436.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
BASE COUNT          514 a          456 c          378 g          734 t
ORIGIN      
        1 cagtagcttt tctactgatt ctggttattt taataaaaaa tctcatcaac cattccttta
       61 cactcattca attatctaat gatctattct tatggtcatt gccgtttctg attatcggcg
      121 gttttttatg ggtcttttct tcgggtttct tcgatcattt tcagcgatcg gtccatcttg
      181 cacgaacaag gaaccgaaag aaaaaaccag aattttcctc gctctcgtct gccagctacg
      241 gtatgtacag cttttggctg atcattgccg gaatactaat tgctctttct gctattttca
      301 tgcttttttc tttgttagga taagtgaata tgagaacgag gttgcgacat ttgccgcagc
      361 ctcgtttttt ctcatgctca cggtattaac gattattctt caacgtatgt ccatttgtaa
      421 tcccatgaag cgcctgccgc atggtgaacg atacctttga ttttttcatt cactaggtga
      481 ccttctgctt tttggtacac cggaacgaca cccatatcat cagagatgat cttgattgct
      541 ttttggaaat catcccaacg ggcttgttca tcgccagcgt ctgttgatga agctgcttta
      601 actgcttcgt catattctgc attgctccag cgaccacggt tgtatgaatt acctgtgaca
      661 aacaaatcag taaagctgct tgggtctgca tagtcagcag cccatccgcc tagtactgcg
      721 tcaaaatcac ccgaagttga gcggtcaaga cgtacagaga atggaacagg tgttggtttg
      781 actgttacgc catccaagtt ttcttggatt gagttttgaa catattcgat tacttttttc
      841 gatgaatcat catcagaagc catcaaatca aattctaaag aatcaacgcc caattctttt
      901 ttcgcttttt cccaatattc ttttgcttta tctttgtcat aagaaactaa ttttccagct
      961 tcttttgcaa agtcttcgtt cgttgttgga ctttttacca tatctgatgg gatcaatcct
     1021 gtagagacaa ctgatccgtc acctagtact tgttttacta aagcttcacg atcgatagaa
     1081 taagaaatag cttttcttag gttcacattg ttgaatggtg aattcttttc acgttgattg
     1141 aattcgatat agcttgtccg cgcttcttta acagaagtat aagctggatc gtttgcgttt
     1201 tgctgtgcta actcaccaga caagatcacg tcatctgctt ggccatcttt gaataagttc
     1261 aatgcagtag aagattcttt tactacactg actttgattt cagaaagttt tactgtatct
     1321 ttgtcccagt aatgatcatt tttcttataa gtccattctg tatctgttcc tggtccatca
     1381 aaatcttcta atgtgaatgg tccattataa acagctttat cacttgcaga tgcataggca
     1441 tccccatttt cttccactac ggattgtttt tgtgggaaaa atgatgggaa agcaagcaga
     1501 tattggaaat atggtgtttg tttcgtcaat ttgatttcta attcatagtc accaactgct
     1561 ttgatcccta gttctgattt atctttcttt ccagctgtaa tgtcttctgc attttcaact
     1621 ggcgcataca ggtaagcata ttcagaagct gtagctgggt ctactgttcg ttgccagcca
     1681 taaacgtaat ccgctgctgt aacaggatca ccatttgacc attttgcgtc ttctcgtaat
     1741 ttgattttgt aagttaatcc gtcttcactg acttctgcca actcgctagc tcctgccggc
     1801 tgtggtttgc tgtcttcatc aagacggtaa attccttcat aaacgttatt caatgctgta
     1861 aaactgattg tatctgttgc aactgataag tcagctgtag gcatttcttg aggcacgact
     1921 aagttaaata cgcctcctcc atctgctgaa ccgctttttg ctgaactact gcttgtggct
     1981 gaactgccgc cataacatcc agcaagtaca aggccgcata ccgcaaccaa accaaatgta
     2041 aatttctttt tcattttacg taccccttta ttggaatttt ca
//
LOCUS       DAZUMK010000218         2058 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000218 DAZUMK010000000
VERSION     DAZUMK010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.227"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(101..708)
                     /locus_tag="ACG808_002871"
                     /pseudo
     CDS             complement(101..708)
                     /locus_tag="ACG808_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            1231..1905
                     /locus_tag="ACG808_002872"
                     /pseudo
     CDS             1231..1905
                     /locus_tag="ACG808_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
BASE COUNT          739 a          326 c          356 g          637 t
ORIGIN      
        1 tatgaatata aatagagtgt atcttctttg ataaatgtca gaaaaagtgt gacaaataac
       61 catcagtaaa attacttgtc atactattga taaggagtat ttaattgaag agaatgatta
      121 ggagaaagga tctccgtact ttttatagta atcatagaat ccaagtttgt atagacgaat
      181 ctttttttct cttatttctt ctgtttcaaa atggactgct tgaagaatca agtctgtgaa
      241 ttctatagga gctgttccat tcgcagtaat caaattacga tctttcacag cttgttgttc
      301 tataaaatct tctttattct gataattttc cgattctaac caaggaaatt gcgcatttcc
      361 ggtgtgtttg taaccttgca ataatccgct ctgagctaaa taatcaactg ccccacaaat
      421 agcagctact ggtacgtctg tttttaacaa tttatcgatt atttgatata aatgatgatt
      481 ttttatgtcc catgaattgc caccgatcat aataaaaagg tcaacttttt caggtatttg
      541 ttcgattaag taatcgattt ttgtttttaa tcctccaatg gaagtgacct ctgattggat
      601 agaagcagtt ttgttttgcc atccctcttt ttggtttagt gtgctagtaa tataagcacc
      661 ttcccaatcg gcatattcct ctaatagaaa cgtaatcgca gttttcattt tcaagctctc
      721 ctttactatc tatgacaagg acaacagttt taacatacag acattcatta aatttggata
      781 gcgacagcga aaacaacaat tcttaagttt tagacagagt atacagacag tcaacttaat
      841 taaatacgaa ctaaataaaa atgacaggta atcatttttg tttaccagca ttataacatt
      901 ttgcatacat ggatgacaaa agaaccaatt cccacttgct tgagtacaat cttaaaagaa
      961 actggaaggc aaaaagtaat tatagaaacg aaaaataaat catatcgggc actcagacat
     1021 gaaaacaacc ctagaaatat ataactagtc caatgaaaaa aaagcgaatg ccgtagaaaa
     1081 caaggattct gcgtacattc atatctcatt ttgcgagcat atctgctatt actgtgacta
     1141 aatgttaata tggttaaaat gaccgttata ttttttatat aaatatcgta taattagtat
     1201 gaatatagaa gaaggaaaga aggtgcaatc ttggaaccta aagacaagta tttagattac
     1261 gctagggtca ataaagataa atttattgaa acaatcatca agggaaaaat ccctgatgat
     1321 gaaaaagatg cggtattcat ggctggaagc ccaggagcag gtaaaacaga ggttgcacta
     1381 ggtttagctt aaaattatga caaccatgtt gtgattgatg cggactattt tagagcacaa
     1441 tttccagaat acaatggaaa gaactcaagt gtttttcaga aagcttcttc ttggttagtt
     1501 gaacaatcat taaaatatgt actggaacat ggctattctt tcatactaga ggatactttt
     1561 gctatcctta gtgcagagaa aaatattatt cgagccctta aaaataactt tcgcgttacg
     1621 attttctatg tttaccaaga tcccaaagtt gcctgggatt tcactcgaaa acgtgaacta
     1681 gctgaaggac gtcatgtgcc aaaagaaatg tttatcaatg cgttttttaa gtccagagag
     1741 aatattgaaa aagtcaaaaa tagacatcct gaagtagtcc ttcacataat gattaaggat
     1801 tatcaaaacg acatttctga agtgcattat gcagcagata atattcaatt agtactacca
     1861 atacaatata cttcaaaaga gttggaggag gaattacatg actgagaaag aacaagtaca
     1921 acaaatcgtg aagaagtata ataaaagtat cgctgattta tcagaaaacg cctcagcgaa
     1981 agaatttaaa acagtaatga attacgtagc agatgaagca aatcgtaagc agcgaaaact
     2041 agttgggttg gataaata
//
LOCUS       DAZUMK010000219         2042 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000219 DAZUMK010000000
VERSION     DAZUMK010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.228"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(482..859)
                     /locus_tag="ACG808_002873"
     CDS             complement(482..859)
                     /locus_tag="ACG808_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048437.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(1159..2040)
                     /gene="galU"
                     /locus_tag="ACG808_002874"
     CDS             complement(1159..2040)
                     /gene="galU"
                     /locus_tag="ACG808_002874"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFS5048438.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          597 a          435 c          313 g          697 t
ORIGIN      
        1 gcattgaacg aatacgccca ataccttgac atagcaaata aaacaacgtg cagaccatgg
       61 aaaatatcta cataggggta gaattttcac cctagattag gtacaataaa ttaggtcgat
      121 gcactgcatt gactatggca aaatgatcgg tcagggtgta tgtgtgtgac tggtcattcc
      181 ctgattctta gcgccaacta agggtcaggg agcctttttt tattcagctg tttctatctg
      241 aatttattgt aagtaatctc ttatccaagt gcaagcaaga aaatacaaaa aaacattggt
      301 atgatttata tatatataaa taacataaaa aaaggaatgg cttatcccga tacaacaagc
      361 attcctttaa aaaagattct tttatataag tgaaaaaagc ttttttccac caattaaata
      421 aatctctcat ctcaatagtt tatccattat tttatctttt tttgcgtata cctactaaat
      481 attaccgtct tcttctcgtt cttctacttt gttcgagaga atctgtacta gaaaaagtag
      541 ttgtatgtct accttttctg cctatccgag actttctttt catatatggg atataaatga
      601 ttagatacag tacagcaaaa aataaagcaa atacgcaagc ttcccaacta cctaacgcca
      661 attcaaccat caaggtagca accccaacgg atgctgcata ccagataaaa atcagaaacg
      721 tttctttttt tctgaccatg cgaaacatga taaatagact aataagcaat agtggcaaca
      781 caaatatcca attgcttcct tccatcagcc acttgagtat atcaagcagc gactgtgcaa
      841 cagcgactaa ttttcccata gtgaacacac accttttttt ataattaaaa aattcgcctt
      901 tttattctac tatgaatctt ctggcttggc tttcatttct cttatgtaat cattttcttt
      961 tatacaactt aaaaaaaaat taaaccatta gcataatatt ttctgaatct attcacgaaa
     1021 gtctttttct atctttcgtt ttccacgact caactgatct agagtcaaaa aactaaaacg
     1081 acagttgcgc aaatgacgca caagctgccg ctttaataac aaatagcctt tcattctcag
     1141 caggccactt atttccagtt aactttctgt tcttagcttt ttactcaact ggataatata
     1201 tgcacttaat gaagtcccaa tttccgaatg ttgaagtcca tattcaatat ttgtcttcat
     1261 atatcctaac ttatcgccta cgtcgtatct ttttccttta aactctctcg catagaccgc
     1321 ctctgtctgg ttaagagtct gaagtgcatc cgttagctgt atctcgtttc ctgctcctgg
     1381 ttcttgagtt tctaatatct caaatatttt aggagtcagt agataacgtc cgataatggc
     1441 taaattactt ggtgcttttc ccatttcagg tttttctaca aaccctttta ctctgcaacg
     1501 acccttttcg ctatctgttt taggatcaat aattccgtat ttagatgttt cattttctgg
     1561 tacacgcatg acggcaacag cggaagcttg tgtttcttca taatccatca tcaattgttg
     1621 agtcaatggg atatcatctt tcatcaaatc atcccccagc atgacgacaa acggttcatt
     1681 tcctataaat gcttttgctt gaagcacggc atcccccagt cctcttggat gagattgacg
     1741 gatgaagtga agattgactt gagtcgtttc ttcaactaaa gcgagtaatt ctgttttttc
     1801 cttttcccgc aaattgtttt ccaattcgat atttgcatca aagtgatctt caatgggtct
     1861 tttttctttt cccgttacga tcaagatgtc ttcgattccc gaatccaatg cttcttccac
     1921 aataaactgg atcgttggtt tatccacgat tggaagcatt tcttttgcca tggcttttgt
     1981 tgccggcaaa aaacgtgttc ctagcccagc ggcaggaatg actgcttttt tgaccttcac
     2041 tt
//
LOCUS       DAZUMK010000220         2036 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000220 DAZUMK010000000
VERSION     DAZUMK010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.229"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..153)
                     /locus_tag="ACG808_002875"
     CDS             complement(<1..153)
                     /locus_tag="ACG808_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFS5048439.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
     gene            332..844
                     /locus_tag="ACG808_002876"
     CDS             332..844
                     /locus_tag="ACG808_002876"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5048440.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            820..1878
                     /locus_tag="ACG808_002877"
     CDS             820..1878
                     /locus_tag="ACG808_002877"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS5048441.1"
                     /translation="MGGPRGRMISSSDRKQAVELIQEANRNGARLTYACNELNISVRT
                     YERWTREGTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLAD
                     CSIYIASESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPI
                     KGLFYRLYLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGS
                     PMKAATFQVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARK
                     WTKEFVDWYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVR
                     NWTPHQSVALNPLKETES"
BASE COUNT          722 a          394 c          410 g          510 t
ORIGIN      
        1 cacccaacga taaatcgtag tatgacaaac atttattcca cgatcatata acaattcctg
       61 aacttcacga tagcttagat tgtaacgcag gtagtaacca acagcgacaa taatgacgtc
      121 ttttttgaat tgtttgcctt taaaatgatt cattactctg tcctctctgt cttttttctc
      181 aattttacac taaaatagat tttttggaaa actttgcaac agaacccttc aaacaaaatt
      241 taaagctaaa gcactacaag accccctatg ttaggctagt tgtcgtatag gataataagt
      301 tataataaag actatacgga ggatgattac catgacaaat aaaaacaatg aacgttactc
      361 taccgaactg aaagcgacag ccatcaagaa aatgatgcca cccgaaaata gggggattcg
      421 ccaattaagt caaactttgg gcattccaga agcgactctt tattcttggc gtaaaaaagc
      481 tagaattcaa gggaatacta cacccggaac gaaaaaaaat gtcgaaaaat ggtcaagtga
      541 agataaattt ttaattgtga tggaatcatt ctcaatgaac caagtagaac ttgcggaata
      601 ttgccgaaag aagggcttat ttaaagaaca aattgaggct tggaagaaaa cgtgcttatc
      661 tgcgaatgaa caggaagaaa atcgaacgag agaacttgca actgaactaa aagaagagaa
      721 aaaacaagcg cgtcaattag agaaagaatt acgtcataag gaaaaagcat tagcggaagc
      781 cgctgctctc ttattactaa gaaaaaaagc acacgcgatt tggggggacc aagaggaaga
      841 atgatttcat cttcagatcg caaacaagct gttgaactta ttcaagaagc caatcgcaat
      901 ggggcccgtc tcacctatgc ctgtaatgag ctgaatatta gcgttcgaac ctacgaacga
      961 tggacgcgtg aaggtacgat agcgcatgat caacgccctt tggcgaaacg tcctgtaccc
     1021 aaaaataaac tgacggatca agaacgcgaa aaaataatag aaaccgtttc aaaaaaagaa
     1081 tttatgaatt tgccacctag ccaaattgta ccaaaacttg cggattgctc gatttatatt
     1141 gcatcagagt ctagcttcta tcgtgtattg cgagaaaaaa atatgcaaca tcaccgtggc
     1201 cgaagtcaag tttcacaaaa acgcataccg ccaagtcacc ttgcgacgaa gccaaatgaa
     1261 gtttggactt gggatattac ttggctaaaa ggtccaataa aaggactctt ttaccggtta
     1321 tatctcatca ttgatttatt tagccgtaaa atggtaggct gggagatttg ggaatcagaa
     1381 gaagctcgtc atgccgaaaa acttattaaa cagacggtgc ttaaggaacg tatccaagga
     1441 cagccgttgg ttttacactc cgataatggc agccctatga aagccgcgac ttttcaagtc
     1501 ttactcgaaa aattaggaat tcaaagttct tattcaagac cacgagtgag taatgacaat
     1561 ccttattcgg aagcggcttt ccgaacgtta aaatatcgac cagaatatcc caataacggc
     1621 tttaaaacaa tagatgatgc tcgaaaatgg acgaaagagt ttgtcgattg gtataacgat
     1681 tctcatttgc atagtgggat tcaatttgtg tctcctgcag cgtgtcatga aggacgacat
     1741 ctcgctattt tagagaaacg aaagcaggtt tacgaggacg ctaaaaagcg ccatcctgaa
     1801 cgctggtcaa aaggtgttcg aaattggact ccacatcaat cggtggcgtt gaatccttta
     1861 aaagaaacag aaagttaaca ggagcataat ctttcaagat tatcaaataa ccctcgtcac
     1921 ccaatagggg aaaaagttat tcttccccta ttggataacg aggccaaccg accataggga
     1981 ggttctgctt tttgataggt ggaaaataaa aatacgacaa ctatattgac aaacac
//
LOCUS       DAZUMK010000221         1940 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000221 DAZUMK010000000
VERSION     DAZUMK010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.231"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            109..>1940
                     /locus_tag="ACG808_002878"
     CDS             109..>1940
                     /locus_tag="ACG808_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS5048442.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETF"
BASE COUNT          761 a          265 c          336 g          578 t
ORIGIN      
        1 tgaaataatc aagaatatta gaaacttcta cattaatcaa gaactttctt ctgtatgaaa
       61 atagagataa taattgtaca cttaagctgt attcaggagg tgaagataat gggcatcgaa
      121 tttagcgaac ggcaaatgaa aatactatac tttttgattg aaaatgagca cacaacttac
      181 aaagaattat cagaacaatt atatgtatct acgaaaacaa ttagcaaaga tattttaatt
      241 ttgaaagaaa taatccaaaa aatgccagat gtacaaatgt tggttcaacc taaaaaaggc
      301 gtttcgctta ttggaaaaaa agaaacactt cgaaatttat tatccagtca taaaatcaat
      361 atcgcagact ctccggagga ccgtctaact tatgtgtttg ctaaattatt gaatactaat
      421 gattctctaa agattcaaaa tcttgctgac gaattatacg tgagcaggtc gacggtagaa
      481 agcacaatga aagatgttag aaaaagacta aacaagcatg ggatgtcctt agtttcagat
      541 cgaaatggcg tgcgcatcaa agctgatgaa aaaagtaaaa gaaggttgat gtcagaagtt
      601 gtgaactatt attggggtgg ctttacagca tctaacagta aaacagatct cttagacttg
      661 aatatcaatt tcattggtga agcgaataat ttcttaaata aggacatatt gaaaaaagta
      721 gccgaattgc ttaatcgata catgaataag acacaattga aatttacaga ttatgaattt
      781 caaactttag ctatccacct tgctattgcg atagaaagga ttgaaacaag caatttctta
      841 tcacgtagta ataattctga agaagaaatc tcaaagaata cacttatttt aatcaaaatg
      901 attgaaaaag cattttcaat tattgtgcca atagatgaac aagcttatgt tgacattcac
      961 attacttcga ttgaaaaaaa tgctatgaat cacgacttag atcaaacgaa gctttcctat
     1021 ttagatatgg gtgttgagct acaagagtta gtaagaatta aattaaatga attttatcct
     1081 gatgaagaat tgatcaataa tctagtatta catctcaatg cagcagttaa aagactcaaa
     1141 ttgggagtaa atatctataa cccttatacg gacaaaatca aatattcttt taaaaggtca
     1201 tttatgataa gtgtagattt attagaggaa atcgaagaga gattctgtat acattttaat
     1261 gaagatgaaa tcgcttacgt aacactccat attcaatcac ttttggatcg ttacaaacct
     1321 gacaaaacga aagtgatatt agtttgtagt agcggatatg gaacatcaaa attattagag
     1381 caaagaataa ataacggttt tgcagatatg gttgagatag aagatgtact aagcatcaat
     1441 gaactacaag atagaaaagt cactgatgaa cttgttataa gcacgattcc gattgaaaat
     1501 acaaattttc ctgttattgt tgttagtcct ttgatgttag gggcagatgt cagaaaagta
     1561 aaacagtatc tacagttaaa tgaacaaaca gaagatgctt ttctaaattt attagataaa
     1621 cgattttgct tttttaccca ccaaataaag gataatcaag tgaacgtttt aaatataatt
     1681 accaaaaaat tggtaaaaga aaattacgcg aaagaaggga tactagaggg agcaatcaaa
     1741 agagaaaaac tatcttcaac agcgatgaat gattttgcta tgccgcatat cgaagtagat
     1801 tttataaaaa agccagtaat ctgtgtctac gttaatccag aaggaattga ttgggatggg
     1861 acacttgttc attctgtttt cttttttgca cttaatgaaa aagtaaaacc tgaattgaat
     1921 aaaatttatg aaacattcaa
//
LOCUS       DAZUMK010000222         1934 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000222 DAZUMK010000000
VERSION     DAZUMK010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.232"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(75..1001)
                     /gene="cysK"
                     /locus_tag="ACG808_002879"
     CDS             complement(75..1001)
                     /gene="cysK"
                     /locus_tag="ACG808_002879"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFS5048443.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLGTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(1141..1934)
                     /locus_tag="ACG808_002880"
                     /pseudo
     CDS             complement(1141..1934)
                     /locus_tag="ACG808_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
BASE COUNT          527 a          441 c          334 g          632 t
ORIGIN      
        1 tcataaattt ttgtgtgttc ttttcatctt taaaagtttt ttacataaac ctatctggag
       61 aaaaaccaag tgccttatat ctcttggtaa agggctgtag ataaataacg ttcaccattg
      121 tcgggaacca cagctaatac cttcttgcct tttcctaatt cttttgcgac ttttagcgcg
      181 gcagcaattg cagcgccaga tgagatgcct actaaaatcc cttcttttcg cccaacttct
      241 ctagctgttt ccatcgcttc gtctccacta atagaaagga ctttatcata cacagaagta
      301 cccagtgttt taggaacaaa acctgtaccg attccttgga ttttgtgagg tcccgcttct
      361 ttcccttcta aaatagcaga ttctgctggt tctaccccga ttaattcgat tttcggatac
      421 acacgtttta attctcggcc agcgccagtg atcgttcctc ctgtaccgat acctgcaaca
      481 aacgcgtcca agccatttac tccaaatgct tgatgtatct caggaccagt cgtcttctca
      541 tgcacaagag gatttgcttc attttcaaat tgtaatggta aaaagtagcc gttttctttt
      601 gccaaacgtt ctgcttctct gattgatcca gaaataccat ccgcaccagg agtcaaaatc
      661 aactctgcgc cgtacccttt catcaataaa cgtctttcga tactcatcgt ctcaggcatc
      721 acgatgatta ctttatatcc ttttgcaact cctaccatcg acaatccgat tcctgtattt
      781 ccagaagtag gttcaatgat agtatcacct ggtttcagta gaccatcgtg ttctgctttt
      841 tctatcatac ttaaagcaat acgatctttt acacttcctc ctggattaaa aaactcaagc
      901 tttacaaaaa catctgctga atcttctggt acgattttat ttaatttgac gattggcgtt
      961 ttgccaatca attcggtgac tgagttatag atagctgtca tcttcaattc ctccttgtct
     1021 ttgctcttct tacttatcat aatacaatct gttcatttat ggcgagtgat tcgtctttga
     1081 ctagcaaaaa aaagacaaag atataccata tatggtagtt tctttgcctt tttatctaaa
     1141 tcaaacgttt agttcttgac caactaataa agaataactg gacatagaca tgccattttt
     1201 cgccattaga tcatcaacag acatgccaaa tttttgtgca atatcccata gcgtatcacc
     1261 tgacacgact gtatatgtct gaccattatt tgtatttaca tctgttgttg aagatgatga
     1321 cgttgaagtc gtcgtagtag tcgttgttgt tgtacttgtg gtagccgaac ctgttgatgg
     1381 cgtatcaaaa cgggttaaat tataagtatt aatcaatgag ttcaatttgg ccccataatt
     1441 tggatcggta gcataacgac ctgtcaagta cgctgttgca tcataaaaac ttgttgtatt
     1501 gcttttccac acgccagcat aatgataatc gcccgtggaa aaactggttg aacgaagaac
     1561 attcgcatgg tcttggaagg attcccaata agaagaatat tttcggaatg gttcattcat
     1621 cgttacccat tgtccgttac atattctttg gttgccatat aaacgacctg tccgttatag
     1681 cttcctttca caccgaatag attataattt ggcgcactag ctaatccgga agtgccatat
     1741 gcactttcca acaacgcttg agcgatcatg actgaagcat acaagtcatt acttgcagca
     1801 acttctgacg cagaccagcc gatttgatcg ataaacgctt gaggatcagc aactgtttga
     1861 gatccttgtt catctgcatg aacatgttgc ggtaccgctg taaccatcat cggcccaact
     1921 aaaagactcg ttcc
//
LOCUS       DAZUMK010000223         1928 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000223 DAZUMK010000000
VERSION     DAZUMK010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.233"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            198..1163
                     /locus_tag="ACG808_002881"
     CDS             198..1163
                     /locus_tag="ACG808_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFS5048444.1"
                     /translation="MSKKGIIVNEWEKLNRISNRRLNVQNLNTPLKLQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMQLNEGNGFVKSVGKNGWQIVDQYEENIAYIELLQF
                     HEGKARIDFNPNKIGQFLASSMKNFIHDLFLEPHFSRADIACDMVNVPDEFIMQYRVV
                     DPVSFKPIYGRSGKLESAYWGSRASERQIRLYNKKLEQETKRKVVPKEVETWWRLEMQ
                     LRRGKATDWHNMVQESLNSFASLHFLPIDTTPTDRIMLAGLMSDQNFWSLLSRNSKYK
                     YRELLKEESQNDELTNHLRETFAESADNLKQELDTWLLGLDVTEK"
     gene            1363..1545
                     /locus_tag="ACG808_002882"
                     /pseudo
     CDS             1363..1545
                     /locus_tag="ACG808_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          630 a          294 c          438 g          566 t
ORIGIN      
        1 aaaaaaatag tcgagcagtg ttgacgcact ctcgaccttt acggaaacta cttcacctag
       61 ttccgtaact caagttcatt atacgaatat ttttaggttt cttcaagcta gagatttttg
      121 gatgtggatt tttagagtta cgtgaactct ggaaattctt tccaagaatc tcttttttcg
      181 ttgaggagga gctatttttg agtaaaaaag gcataatcgt aaatgagtgg gaaaaactta
      241 accgtatttc taacaggcgg ttaaatgttc aaaatttgaa caccccccta aaattacagt
      301 ggtcaatcga ccgaattacc attgttggaa aattgaaaga aaatatttat tatcacacac
      361 caaatgatgt cttgatttta aattttgagc aattgatgca attgaatgag gggaatgggt
      421 ttgtcaaatc agttggtaaa aatggctggc aaattgtcga ccaatacgaa gaaaatatcg
      481 cgtatattga gctcttgcaa tttcatgaag ggaaagcacg aattgatttt aatcccaata
      541 aaattggtca gtttttagcc agttcgatga aaaatttcat tcatgatttg tttttagaac
      601 cccatttttc gcgggcagat atcgcttgcg atatggttaa tgttccggat gagtttatta
      661 tgcaatatcg tgtggttgac ccggtatcgt tcaaaccgat ttacgggcgt tctggaaaac
      721 tagaatcagc ttattggggt agccgtgcca gcgaacgaca aattcggcta tacaataaaa
      781 aattggaaca agaaaccaaa cgaaaagttg taccaaagga agttgagact tggtggcggt
      841 tagaaatgca actacggcgt ggcaaggcaa ccgattggca taatatggtt caagaaagtt
      901 tgaatagttt tgcgagcctg cattttttgc cgattgatac aacacctaca gatagaataa
      961 tgctcgctgg tttaatgtct gatcaaaatt tctggtctct tttgtcgagg aattcaaaat
     1021 ataaatatcg ggaattgctg aaagaagaaa gtcaaaatga tgaattgacg aatcatttgc
     1081 gagaaacgtt tgcagaatcg gcagacaatt tgaaacaaga attagacacg tggcttttgg
     1141 gtttggacgt gacagagaag tgataccgaa taagaaaagt cagatgagtg agagtaaatt
     1201 tacgtcaatg tttaatgacg tgagtttacg ataagcgatt attttgcgtg aagcaaaata
     1261 aagcgtaaaa ggagttgaga gcgtgacacg ctttcggctc cttttttcgt tgtcacttgt
     1321 gacagcgaat accctcggag agcatttgga attgtgacag ccatgtcaca attaggaaaa
     1381 tgttactctt tgtcaaagag taacagaaag gtttacactc tttttattag cgacagcgcg
     1441 aaggaggggt ttaacttggg gtttagtatt tcgttcaaaa agggaacaaa aaatacaagc
     1501 atttctcata ataatcgtga gttaactgat agccaaaaaa ttaatagttt gtctggtaga
     1561 aaataattag cagatttaca agtgggaaat aaaaagctga aagaagaaaa ttgcatttca
     1621 aactacattt tctacaataa ctctaaaatg gcatattttg cgttctgagc ggttttagcg
     1681 tttatttcgt ttagttatcg gcacaatcgg tgaaacacgt tagaggagcg aaaatggagc
     1741 ctgtaaggcg actggtagcg gaacggtcgt gttagattgt gtagccgata gctgaacgaa
     1801 ataaatgctg gcagcgccat tctatttcgg caggaggagg ctcaaggggt tcggggaatg
     1861 aaattccctg aggtcttttt attttttcta gaggagtgct ataatcaaag aaaatgagaa
     1921 aaaagagt
//
LOCUS       DAZUMK010000224         1907 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000224 DAZUMK010000000
VERSION     DAZUMK010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.234"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..435
                     /locus_tag="ACG808_002883"
     CDS             <1..435
                     /locus_tag="ACG808_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFS5048445.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="ACG808_002884"
     tRNA            complement(759..842)
                     /locus_tag="ACG808_002884"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="ACG808_002885"
     CDS             1370..1870
                     /locus_tag="ACG808_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5048446.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
BASE COUNT          616 a          360 c          328 g          603 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtc
//
LOCUS       DAZUMK010000225         1859 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000225 DAZUMK010000000
VERSION     DAZUMK010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.235"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..129
                     /locus_tag="ACG808_002886"
     CDS             <1..129
                     /locus_tag="ACG808_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFS5048447.1"
                     /translation="SMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQPMEN"
     gene            624..1223
                     /locus_tag="ACG808_002887"
     CDS             624..1223
                     /locus_tag="ACG808_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5048448.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            1287..>1859
                     /locus_tag="ACG808_002888"
     CDS             1287..>1859
                     /locus_tag="ACG808_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbiH family protein"
                     /protein_id="HFS5048449.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIGD"
BASE COUNT          664 a          290 c          356 g          549 t
ORIGIN      
        1 tcgatggaac gtttcttagt ctcgtctttt gatacttaca accaaaaatt cctaggtcgc
       61 agtcataaag gtttccaaca ggccgaaggc gagctcgaac aaatgctgag tcaaccgatg
      121 gagaattaga aacaatctac agtagggaag aaccattgac acaaaattat tgacgctccc
      181 aactaaaagt gtcaaaataa tgatgaaatc atttagttct tataaattag aaaagagaaa
      241 ataaatattc catatctact ttattggatc cgtaacatga ctaaattctg aatacttttt
      301 aaagcttatc atttggtaaa actgaatata agttaattat atagactgaa aattttgaat
      361 tttcagaata gaataactta aaagtactga caaaattttt taaaggggca atattaatgt
      421 tctgagataa tcaaatccta ccgaaaatat caaaaatcca atgtgacaca atcagattgt
      481 gtctcattcg agggaatcac acaatcccac gtggctctag gcagaattga atttttgctt
      541 ggggcttttt ccttatattg attttgaatg agacttatta tatgttataa tgcaattaag
      601 tatcattgga gagggcgaag aaaatgaaga cggtagaacc tattcgagac aaaaaaaaga
      661 tcgatgcgat gaaggcaatc ttggcttcgg gaaaatatgg ccaacgaaat ttggttcttt
      721 tttcgattgg gatcaacacc gcctatcgca tttctgattt gcgtcaattg aagctggccg
      781 acgtgctgga aatttcacgc ggtcgcgtgg tggtcaaaga tcgattagcg atgaaggaac
      841 aaaaaacagc taaacataat tcggtctata tttccaacaa attgcgaaaa acgattttgg
      901 agtacgttca acaggaattt cctgatcaac tccttcagca agattttgac ggttatttgt
      961 tccctagtcg aaaagggacg aatgaaccgc tcaaccgcca aagtctgtgg cgaatcattc
     1021 atgatgctgg cacggcaatc ggattgaaag acattggccc acattccatg cgaaaaacgt
     1081 tcggttactt tctctataaa caggggacca aaacagaaat cattcagtcc ttgttgaacc
     1141 attcttctca gcgcgaaaca ttgcggtata ttggtattac acaagaagac aaagatacag
     1201 ctgtaaaaag cttagattta taatataaaa gttaagttta aacatggata gttttaaatt
     1261 agtagattga gtgttgggag gaaagaatga gtacattata tattatagga aacggttttg
     1321 atttaagtca tggtttaaaa actagataca ctgatttttc aaaatgggtt gaaaaaagtc
     1381 atagtgaaat attcgataaa atagacacaa tgataaatga aaataatgag gtgagaagta
     1441 cagctgatga taagataggt tggtttaatt ttgaaagtag tttgcatgag ttaattcaaa
     1501 gtccagtctt tgaagattat gtagaagagt ctaaggatta tctcataggt tatggacatg
     1561 aagattggtc agattctgcc catcatgatt ttcaaaatat ggtttatgat tatgttgttt
     1621 ctctagaaga attacaacaa cttttcaagg aatggatacg caaagaaata cagccgacaa
     1681 tagaacataa aattccaaaa taccaatttt cgtccgatgt actattcttt acattcaatt
     1741 atacagatgt tttggaaatt ctatacaagc aaccgaaaga aaacatatat catattcatg
     1801 gcagttatga cgatataata atcggccact ccgagaattt tgacaacttt ataggagat
//
LOCUS       DAZUMK010000226         1843 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000226 DAZUMK010000000
VERSION     DAZUMK010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.236"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(372..1541)
                     /locus_tag="ACG808_002889"
     CDS             complement(372..1541)
                     /locus_tag="ACG808_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFS5048450.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          623 a          286 c          254 g          680 t
ORIGIN      
        1 aaggggttta gtttatgatt ttatatcatt tcagtaactc agaatattcc gacttttttg
       61 tacaaaaaaa ttaaccttac ttatatgaag attctaagct cttatttaaa ttttagacgg
      121 gcagttatcc gtagaagttc ttagaatagc taatttacat aattaaatta tatgaaattt
      181 aagtttgaac aatatactaa gaactcccct tactataaag tgaaataaat tagcatatga
      241 ataaccgtgc taccaagctt ttcattaata aaatattttt attaatattt tataacttta
      301 cgcaagatca taattttgtc gggagcgtca ataattttgt gtaaatggaa aagcccattc
      361 tgttgatcaa gttaaaacat tgattctaat gtatcatgta tttctttaaa acctttatga
      421 actcggcata gaaacttttg attatagttg ttgaattgag atactagaaa tctctccaga
      481 gattcttcat taggaaattg ctcttttctt ttggtatatt tctttaattg tttgttaaaa
      541 ccttcaatca aattagttga ataaatagtt cttctgattg acggagggaa attataaaag
      601 gttaatatag caggattcat gagcaattta accactcgtg gatactgctt tttccattta
      661 tctatcataa accttatttg attatttgct tcttcttttg aacttgcttg ataaactaat
      721 ttgaaatcgc tacagatttc ttgtcgatca ctaacacgaa ccttatgagc gatatttcta
      781 gatatatgga cacaacattg ttgaaattga gcatttggat aaacactatg aatactatcg
      841 ttaatgccac ttaaaccatc agttacgact aataaaacct cttttaaacc acggtctttt
      901 aggtcttgaa gaatctcttt ccaaacataa gcagattcag ttggagcaag actaaaccca
      961 agaacctctt tggttccatc taatcgaatg ccaattacaa tataaactgc ttcttttgaa
     1021 acagtttgtc tttttaaagg aatgtgagta gcatccataa aaatgacaga gtatctggat
     1081 tctaaggacc tttctttaaa ggcaataata tcttcagaaa ctattttagt catatttgaa
     1141 atggtttgtg gcgtatagtg atgcccgtac attttttcaa tcaattcaga gatttcagac
     1201 atagtaattc ctttttgaaa tagctgaata atagtggttt ctaaagagtc attggttctt
     1261 ttataagcag gtaaagtttg ttgagaaaac ttaccatttc tatctctagg aattaccaaa
     1321 tttaattctc catactctgt tctgaatgaa cgtgaataat tcccattacg ggaattacct
     1381 gaattgaacc cagcacgatc atatttttca taatcaagaa aagctgttaa ttcagcctgc
     1441 aataatgaat taacagctaa ttctaaatga catcgaaata attcatctaa atcaccttta
     1501 ttgattagtg tttccataat ttctgtagta aaattgggca tgagaaagtc ctcctataaa
     1561 atttcggttg tggtaacttt aattttacag aatggacttt ctttttttct accctaaata
     1621 tctatttaca caaaatattt tacgctatca ttttgtctgt gtagatattt cgttatatca
     1681 ctaaagataa taagatttaa tgacctcatt aatattgttt gctaggtcca aactcttatt
     1741 aaactttctt tgctataaaa cccaaacata gtaataaaag aataacttat tacctttaaa
     1801 ttatactacc aacaaatggt agtatattat taatttatat aat
//
LOCUS       DAZUMK010000227         1778 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000227 DAZUMK010000000
VERSION     DAZUMK010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.238"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            15..497
                     /locus_tag="ACG808_002890"
     CDS             15..497
                     /locus_tag="ACG808_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048451.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            694..1584
                     /locus_tag="ACG808_002891"
     CDS             694..1584
                     /locus_tag="ACG808_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFS5048452.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT          586 a          308 c          370 g          514 t
ORIGIN      
        1 ttgggggaac gttaatgaga agaaaagcag taatcgatcg gctttattta tttttttggt
       61 tttttcctat cgttttgcta ccttttacat gggtaaatgc tgttgtacaa ggtgcggaga
      121 tattaggcct gatccaagga agcggatttg tggtttctag gaattttcca gctttgattg
      181 ttttctattt agtgataagt gtaggtctct tattaccaaa atctctgatc aatcactaca
      241 attggcagct gctttacttt tcttgcttat tagtatttac atctatgttt tctttgctgc
      301 ctcgcttgat tatcggccct aatggtttga gcgagagtgg attgaaggat ttagcttatt
      361 cctccatgta tttcttgacg atcaaaccat ggttctattt agtaattgcc tcatttattc
      421 ttgcaacatt tttttacttc tggacagaaa tcaggataaa aagtgaacga tctgtatcta
      481 taaaaaataa ctattgatgg tggatcatca gtaaaaaaac atatgagctg cgtaaaatgc
      541 atgtttctca tatgcttttt ttattattat gtaaagaatg cgaaaaacgt ttacatgatt
      601 tttacataac ttaactatat ttttacagca accatacgag ggatttacag tcgactgata
      661 aactttagtt gtaaataaga aaggagcaat aaaatgaaaa aagtattgtt tactctaggt
      721 atgatgggaa ttcttttagc cggatgcggc tcaggatcag gaaatacaga tggctctgcc
      781 acacaaaata attcaagtga ctcaaatgaa caagtaaaaa tcgttgcggt aggttcgaca
      841 gcattacagc cgttagtcga tgcagcacaa gaaagctttg tacaagaaaa tccaaattat
      901 caaatctctg tacaaggagg aggaagcgga actggtttga gccaagtcga agctggagca
      961 gttactatcg gaaactctga tgtatttgca gaagaaagag acggagttga tgcttcaaaa
     1021 ttagtggatc ataaagtagc agtggtcggg atggcaccaa tcgtcaataa agatacagat
     1081 gtcaaagata tcaccaaaca ggaattgatt gatatcttta caggaaaaat cactaactgg
     1141 aaggaagttg gcgggaaaga tcaaaaaatc aacgtcgtga accgtgcaaa cggcagcggt
     1201 acccgcgcga cattcgaaaa atggggactg gatggtgcta cccctgtgca gtcccaagaa
     1261 caagattcat caggaacagt tcggcagctg gtaagtcaaa caccaggagc aatcagctat
     1321 ttagctttct catatctcga tgattccaca caagcactaa gtatcgacgg tgtagaacca
     1381 aaagaagaga atgtagcaga taacagctgg ggaatctggt catatgaaca tatgtacacg
     1441 aacggaaaac cttcccctga agtccaaaaa ttcttagact acatgatgac agaagaaatc
     1501 caagaaggac ctgtcaaaga attgggctat ttaccaatca caatgatgga agtagaacgt
     1561 gatcacgaag gaaatatcaa ataactagca gataaacatg ccacggtagt cgttactatt
     1621 aaggaaaaat caactatgtt tccccacata tacatttttc ctttaattgt gaaaaatagt
     1681 tcctcatatt ttgccgtttt ttgagctaat accgagggtc agctgattga acaccgttta
     1741 ttcgaaaaaa agagctacga catagaactg tcgtaact
//
LOCUS       DAZUMK010000228         1749 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000228 DAZUMK010000000
VERSION     DAZUMK010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.239"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      188..354
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            528..1634
                     /locus_tag="ACG808_002892"
     CDS             528..1634
                     /locus_tag="ACG808_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFS5048453.1"
                     /translation="MIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLSWSVIFLAR
                     SVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQFQPSELANL
                     FLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIAWVIFWAAA
                     VPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDSFIDSHGAG
                     YQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIGALCVLFLL
                     FSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGVPLPFVSYG
                     GTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
BASE COUNT          513 a          284 c          360 g          592 t
ORIGIN      
        1 cacataaaac taaaaaatta tcaaagaatc ctggtgcaac tgaaagaatt tgcaacggga
       61 ttctttcaac ttatttgtta tttttttcta aaaaatatta aagattgtta aatttcacta
      121 tttcatttca aattccattg cctaatcatt aggaaaagtc tatcatgaac tcgtaaatag
      181 aatatagtaa taaccttgtt aggtgaggct cctatacaaa cataggctgt tgcccagaaa
      241 cgtcgaaagt cgccaatggg taaaacagaa actgttggat taaggctttt tccaatagct
      301 aagaatgttt tctttacgtt gtatagtgct aaaactcgga cgatgagatg atgctaggtc
      361 agaattcttg ttcgaaaagg tttctgacct tttctttata tttttgtgac tgacttattt
      421 gatgagacaa actcgctcct cacctgacaa gtggattgct gtgtgaggag gagatgcggt
      481 gaaaaaagat cgattggtgg attttgggtc cttatttgac tttgtccatg atcggtcttt
      541 tagaagtata cagtgccagt tcttatcgtt tattgcaagc cgatgaaaat acaaaaagtc
      601 tattattgcg tcaactaatt ttcatatttt tgagttggag cgtgatcttc ttagctcgtt
      661 cagtcaaact acactattta cttcacccta aaatagcagg atacggttta gccttatcga
      721 ttttcttttt agtattagta agaataggga tattcggtgt cactgtcaac ggcgcacaac
      781 gttggatctc tctgtttggc attcaattcc agccttctga actggcaaat ctttttttga
      841 ttttttattt aagctggttt tttcgtgacg gaaatagtag cccaaaagat ctaaaaaaac
      901 cattcctgat tacagtaggt ataacttttc tgattttatt tcagccaaag attgctggag
      961 cattgatgat cctttcgatt gcgtgggtca tattttgggc agcggcggtt ccatttaaaa
     1021 aagggatcta tctaatcgtt actttttctg cattgctgat tggagcagca ggcggggtat
     1081 tatatttagg aaataaaggt tggcttccac agatgtttaa tcatgcttat gaaaggatcg
     1141 cgactttaag agattctttt atcgatagtc atggtgcggg ctatcaaatg acccattcgt
     1201 tttatgcact atataatggt ggcatatttg ggcggggatt aggaaatagt atcacaaaaa
     1261 aagggtatct tccagagaca gaaacagatt ttattttttc gattattaca gaagagcttg
     1321 ggctaatcgg tgcattgtgt gtgcttttct tattgttctc attatgcatg agaatctttt
     1381 gtttaagcag tcgctgtaaa aatcagcaag ctggattgtt tcttttagga ttcggaactt
     1441 tgctattcgt tcagacgata atgaacgtgg gaagtatcgc ggggctgatg ccaatgactg
     1501 gtgtgccatt gccatttgtt agctacggag gaacaagtta tctgatcctt tctttaggaa
     1561 tcggaatcac gttgaatatt tcttcaaaga tacaggccga agagttggcg ctctatcgac
     1621 ctgaaaaaca ataaagagaa actgagaaac tctcagtttg aattatcata aagtacaatc
     1681 ttttattttt caagcgagag gttcttttag aaagaggaat tcgcaatgaa agtatctcaa
     1741 aaaatcata
//
LOCUS       DAZUMK010000229         1745 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000229 DAZUMK010000000
VERSION     DAZUMK010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.240"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            242..1534
                     /locus_tag="ACG808_002893"
     CDS             242..1534
                     /locus_tag="ACG808_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFS5048454.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
BASE COUNT          560 a          294 c          355 g          536 t
ORIGIN      
        1 atgtgtatca atattaaaac tgaaaaagat atggataata ggatcaaagc cctcattgga
       61 acactcaata tattatcaga tttaaatttc aaatagataa tttcattgtt tagtaatatc
      121 gaaagatact tttttaatga tgagagtgta atataaaccg tgtaagtaac cattaatcct
      181 aggcaggtgg ttgcttcgcg gtttttcttt ttctacaata agatcaagag gtgattacac
      241 gatggctaga aagaaaagaa atcctgatgc cgaaaagtta gctgaatcca ttctgaatgc
      301 ctatcaacct gaatctgtcg atgacatgca agatgctttg aaagatgtgt ttgggcccct
      361 ttttgaaaaa atgcttcaag gagaattgaa taatcattta ggttatgatg cccattctaa
      421 agagcctaag gaacacgata accgtcgaaa tggctatgga actaaaacgc ttaaaaccag
      481 ttttggtgaa gtagctattg atgttcctag agaccgggaa gcttcctttg aaccagagtt
      541 aattcctaag agaaaacgag atgtctccga catcgaaggg aaggttcttt ccatgtatgc
      601 acgaggaatg agtcaacgag atattgccgc aaccgtcgaa gctatttatg gctttgatat
      661 ttcccatgaa atgatttcag atatcactga cgctgtcctt cctgaattgg aagaatggca
      721 agcccgcccc ctagcaaagt gttatgcctt tctattcgtt gattgtatgt atgttacttt
      781 acgagaaaat tatgaagcca aagaatatgc tgtatacacc attcttggct atgatctcaa
      841 aggaaataag gagattttag gattatggtt aaatcaaaca gaatctaaaa atcgctggat
      901 gcaagtattt gatgaactga aagcacgtgg tgtcgaagat gtctttttca tttcgatgga
      961 cggtgtttct ggcctcgaag aaggcgcaaa agcgatcttt ccatcggtaa ttgttcagcg
     1021 ctgtattgtt catcttgttc gaaatgcatt gcgctatatt ccaagtaagg actataagga
     1081 agtctgccga gatatgaaaa agttctacgg tgcttcttct ctaaacgctg cacatgctgc
     1141 ttttgacagt tttcaaaatc ggtggtctca ttattctggt gctgtagatg tctggaaacg
     1201 gaactttgca catgttgaac aactatttga ttatggtagt gccattcgaa aaattatgta
     1261 cactaccaat gcggttgaaa gtgtccactc cagcttcaga aaagtcacaa aaaaaggagc
     1321 gttctctaat gagaatgcac tcctaaaact actttattta cgcacgaaag aattacacgc
     1381 gaagtggtca ggtggtcgaa ttcaaaactg ggctatggtg cttaatcagt taatgatcaa
     1441 cgagaccttc tcctctagaa tgaaaagtat gcgatttacc ttccgtaaca acggtaggtt
     1501 tattcaacta tgtgtccgat caaaatttat ctgatttcaa aatagccgag agaattatgg
     1561 tggttaaaaa ctcttaacta tttaattacg gttttcatag tttttggatt caggaggatt
     1621 aaaacgaatt ctataaaaaa agatttggtt taaatgaatg attaaacgac aatttagcaa
     1681 cgtagtcaga gtaaaacagc tctttctttt cgtatttttt aaagttgtta cgaaaaagtt
     1741 gctat
//
LOCUS       DAZUMK010000230         1740 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000230 DAZUMK010000000
VERSION     DAZUMK010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.241"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(136..1560)
                     /locus_tag="ACG808_002894"
     CDS             complement(136..1560)
                     /locus_tag="ACG808_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFS5048455.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
BASE COUNT          497 a          372 c          271 g          600 t
ORIGIN      
        1 acacatcgtg aacggacgta caaataatgg ttttttctta gtttctttat atcgtattct
       61 ttttccgaaa aaaaagaaac agcttgaaat ttaaatccaa gctgttatat tctttaatct
      121 atttactttg tgaaattatt ctgttaacca cttttctaga attttcatct ttgaataaaa
      181 caacggtatt tggttttcat ccagcctact gtaattgatt ctgattccat ttatatcttg
      241 cccaaaaagt agacttggtg caattccaag acccatatct actacttttt ctgctagttc
      301 ggggtctagt tttttcccat cccaagtaat ccaggcatag taaccaccta aaattgaatg
      361 aagcttgaaa aacgattgtg aatctcccca agcgcttatc agtcggcttt tttctttcaa
      421 cttttttctt acatcttgca ttttcaaatc aattgcttca ttaaatacat tagccactgt
      481 gatttgtgta aggatattca tcgaaaactc catcatttct tgcgcttgag ctagttgcga
      541 gataaaagtc tcaggtccta tcacccaacc gattttcgtt gtttttccta aaaccctaga
      601 taacgaaccg acgtataaca cattatccgg atcaatcgtt cgtataggaa ggacttcttc
      661 ttggtttaga aatgaaagat ccgtaaatac atcgtcttca ataattggaa gccgatactg
      721 tcgacacaat tctactagct tttctcgtcg tttttgactc atcacttttc ctgtagggtt
      781 ttgaaaattc gggttgataa gcactgcttt caatttattt ttatggatcg attctttcag
      841 tttttctaag tcgatccctt cttcatccat cggtatccca aataaacgcg ttcctgaagc
      901 tttgaaaaga ggtaatcggt aaaaaaaaga aggtgactct acagctatcc cctctccagc
      961 tgaaagaatc gtttgtaaaa tcaaaaagat tgcttgctga ccgccaccag tcacaagaag
     1021 ctggttgggg gaaaatgcca attcaaattt actttccaaa taccgtttga ttttgttacg
     1081 taacggagta tagcctaaaa gatccatttc tttttcttcc gctaagattt cttcaagaga
     1141 ataagaagga aactcaaaat tcggcaaaag ctcatctggc atctcgctag cgtataaatc
     1201 aagaaacgat gttttttgga tctgctttgc ttctcttatt ttttgctcat accagtccgt
     1261 ctgctcataa cgaggagaga tcatttgccg ccaatcgatt cttggaacag cgaatttccc
     1321 ccattcagtc tgattaacaa accatccgct gccacgtttg ctcgttagta ccccaagctc
     1381 cctaagctct tccaatgcat ggacaacggt ggaacgattc acaccaaaaa gttgccctag
     1441 ttttcgttct gattcaagtt gacttccagg attgatcctt ccgttttcga tttctctcat
     1501 aatctgttcc ataatttgac gataaatcgg tccttgtttt ttctctactt tttcccacat
     1561 aagcttctct tttctattgt ctattttttc ttttgatttt accgaattta cgagaaaaaa
     1621 gaaaaacttt ttttgcaaaa cataagggac tgtgacaaaa atcttatcac agtcccttat
     1681 tctgaataat gatgttcaaa agcttgtatc tgcgataata attccagcca aacaaaacat
//
LOCUS       DAZUMK010000231         1735 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000231 DAZUMK010000000
VERSION     DAZUMK010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.242"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            46..129
                     /locus_tag="ACG808_002895"
     CDS             46..129
                     /locus_tag="ACG808_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002497446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HFS5048456.1"
                     /translation="MGIFSIFVINTVHYQPNKKYDKVRIIV"
     gene            207..1379
                     /locus_tag="ACG808_002896"
     CDS             207..1379
                     /locus_tag="ACG808_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HFS5048457.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
     gene            1497..>1735
                     /locus_tag="ACG808_002897"
     CDS             1497..>1735
                     /locus_tag="ACG808_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HFS5048458.1"
                     /translation="MNKKNIKDSQNFITSKHHINEILRNVHLNTNDNIIEIGSGKGHF
                     TFELAKRCNYVTAIEIDPKLCRITKNKLIEYENFQV"
BASE COUNT          663 a          286 c          297 g          489 t
ORIGIN      
        1 cttgtgctat aattattcta atttcataaa aaggagaatt aatatatggg catttttagt
       61 atttttgtaa tcaacacagt tcattatcaa ccaaacaaaa aatatgataa agtccgtata
      121 attgtgtaaa agtaaaaagg ccatataaca gtccttttac ggtacaatgt ttttaacgac
      181 aaaaacatac ccaggaggac ttttacatga cccaagtaca ttttacactg aaaagcgaag
      241 agattcaaag cattattgaa tattctgtaa aggatgacgt ttctaaaaat attttaacaa
      301 cggtatttaa tcaactaatg gaaaatcaac gaacagaata tattcaagca aaagaatatg
      361 aacgaacaga aaaccgacaa agtcaacgaa atggctatta tgagcgcagc tttacgacac
      421 gtgtaggcac gctagaatta aaagtaccca gaacacgtga tggccatttt tcacccacag
      481 tgtttgaacg ttatcaacga aacgaaaaag ccctcatggc ttcaatgttg gaaatgtatg
      541 tatcaggcgt ttcaactcgt aaagtatcaa aaattgtgga agaactttgt ggtaaatccg
      601 tctctaagtc cttcgtttct agcttaacag aacagctaga acctatggtt aacgagtggc
      661 agaatcgttt attatcagaa aaaaattatc cttacttaat gaccgatgta ctctatataa
      721 aagtacgaga agaaaatcga gtactctcaa aaagctgtca tatagcgatt ggaataacca
      781 aagatggcga ccgtgaaatt atcggcttca tgattcaaag tggcgaaagc gaagagacct
      841 ggacaacatt ttttgaatac ctaaaagaac gcggtttaca aggtacggaa ctcgttattt
      901 ctgatgcgca caaaggatta gtctctgcca ttagaaaatc cttcaccaac gtaagttggc
      961 aaagatgcca agttcacttc ctaagaaata tctttaccac cattcctaaa aaaaattcaa
     1021 aatctttcag agaagctgtt aaaggaattt ttaagttcac agatattaac ttagcgcgtg
     1081 aggctaaaaa tcgattgatt catgattata tcgatcaacc aaaatattca aaagcttgcg
     1141 catcattgga tgatggattc gaagacgcct ttcaatatac cgtacaagga aattcccaca
     1201 atcgactaaa gagtaccaat ctaattgaac gactgaatca agaagtacgc agaagagaaa
     1261 agattattcg catcttcccc aatcaaacat cagccaatcg cttaattgga gccgttctta
     1321 tggacctaca tgatgaatgg atttattctt caagaaaata catcaatttt gataagtaga
     1381 aatggtaaaa acattgtata gcattttaca caggagtctg gacttgacta aaaataagtg
     1441 gttataatgg attgttaata taaacattca ttataacctc ataggagtgg gttataatga
     1501 acaaaaaaaa tataaaagat agtcaaaact ttattacttc aaagcatcat ataaatgaaa
     1561 ttttgagaaa tgtacattta aatacaaatg ataatattat tgagattggt tcagggaaag
     1621 gtcatttcac gtttgaatta gctaaaaggt gtaattatgt aaccgccatt gaaatagatc
     1681 ctaaattatg taggataact aaaaacaaac ttattgaata tgagaacttc caggt
//
LOCUS       DAZUMK010000232         1712 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000232 DAZUMK010000000
VERSION     DAZUMK010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.243"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            11..838
                     /locus_tag="ACG808_002898"
     CDS             11..838
                     /locus_tag="ACG808_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5048459.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            complement(1111..>1712)
                     /locus_tag="ACG808_002899"
     CDS             complement(1111..>1712)
                     /locus_tag="ACG808_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS5048460.1"
                     /translation="YGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINS
                     SSTALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNT
                     ISSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSK
                     FKIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
BASE COUNT          581 a          264 c          308 g          559 t
ORIGIN      
        1 ggaaattgtt ttgattaaag tagatggagc attactaaaa gaataccgaa agaaaaaggg
       61 gttaacacaa gtacaattag cagaaggcat ttgtgaacaa gcaactgtca gcaatatcga
      121 aaacaagaat atgtcgccag gcatcaatat cttaagtgcg ctttgcaaaa agctgaatgt
      181 cgagatctcc gatatattag tagaaacaga agaagacaaa atcaggaaaa aaataaatga
      241 gattcaatca ttgaattctg tatcaaaata ccaagaatct tataaaattt tacaaacgat
      301 cgatcaggaa aaagtaagta attctgatct tttaaatcaa attttatttt atcaaggact
      361 gcttgatcat ctttatgcga aaaaaccaga tgcagcatta ttttatttta atcaagtgtt
      421 ggaacaaacg accgaaacag atatccatca cttacaagca gctgcaaacg tggcaatgat
      481 ctacttgtca aaaggagaat tagattttgc gaaagtttat gtggagcgta cacttaaaat
      541 attgagtgag actgattttg ataatctgat ggtctgcatt gtatattatg atatcgccac
      601 ctattaccgt aaaatcgagg attatgataa agcaatccaa ttatgtgaga agggtattga
      661 gtataataag aaacataaat cgacttatgc attggaatac ttgctttatg aaattgcttc
      721 ttgccataag caattaggag aagatgatta tttagaaaga tatatggacg caaaaaaaat
      781 agcgcgtttc aacggaaatg attacgcggt aaaggtgatt gaaaacgacc tgaaataaag
      841 tggttgtgaa atcagtgttc tgatttgaat accgtttatt cgaaaaaagg aggtgtgact
      901 tttgtcatac ctcctttttt cgatattgtt tgattcctat ttaggcaaca tataacggtt
      961 ataaaagaag caatagatta tgatggtatc gattggatgg atacatcttg ggattccttt
     1021 atcaatttga gcgtagcgtg tttttcattt atcgataaat ttgttattca tttttttgat
     1081 aatcctattt acatatttac gtcctggttc ttaaaatgaa tctacttgaa tggtttttat
     1141 ccccgtattt ctgatctcct ctaatacttt ttcagaacaa aatttatcag tgatcaaata
     1201 atcaaagact gaaatatcag caatcttgaa ctttgatgtc agtccgattt ttcgatgatc
     1261 agcgactaaa atcttacatt tgattgtctt gttgaccatc atttcgttga tcgttgattc
     1321 atttaatatc tttgttgtga ccccatgtac tacatcgact ccgctacagc caatgataca
     1381 aatgtctgca ttgactgaag aaatcgtatt taatgcgatg tcgcccacta gtgcttcttt
     1441 tggaaagcgc atctctccac cagttaaaat ataactacaa ttatctttgt gcggagagta
     1501 attgattttc acgttgtttg taatgactgt caaatggtta gattgaagaa aatccagtgt
     1561 tttcaacgca gtcgagctag aattgataaa cagcgtatca taatcgttaa tgtatttact
     1621 agcttctctg gcaatcgctt gtttaatcct ttcaggacca gattgatcaa aatcagtctg
     1681 gtttttgtaa ttataagaac aataaccgta at
//
LOCUS       DAZUMK010000233         1663 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000233 DAZUMK010000000
VERSION     DAZUMK010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.244"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..835)
                     /locus_tag="ACG808_002900"
     CDS             complement(<1..835)
                     /locus_tag="ACG808_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFS5048461.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SS"
     gene            complement(994..1260)
                     /locus_tag="ACG808_002901"
     CDS             complement(994..1260)
                     /locus_tag="ACG808_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5048462.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1250..1606)
                     /locus_tag="ACG808_002902"
     CDS             complement(1250..1606)
                     /locus_tag="ACG808_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS5048463.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          476 a          319 c          200 g          668 t
ORIGIN      
        1 aagaagaatc gtattcttcc tttaaaattc ccgatttact taatttttct agtacatcaa
       61 atactctatc actcaagtaa ctccttaaat tgttatcact ttcccattga actacttttc
      121 tataagttga attatcagtt gaaaagatag aattttcatc cataaacgca tatattagca
      181 gcaatctttc atcattgtca aattctattg gatcaaaaat atctaagttg tcatcaaaat
      241 ctttatcagt tccggtaact tttttgttaa ttactgaagg tgtcttgtaa gaaatagaat
      301 ctaaatcaaa gcgttttgat tctaaattat tttgtgcttt ctcaattaaa taattcactt
      361 tttttgtgag aaaccttctg atattgtctt tgctatatgt tccataaaaa cccttatctt
      421 ctaaagagtc cctgtacttc ttaacttcta tatactcttc agccaaaact tgtggaactg
      481 gttcatctaa gaaaaaaacc cctacctcgc ctttgctatt tttataaaag taatcaattt
      541 ctgctatagt tcccgatcta taattatcaa ctggagaacc caacttatgg ccaaaaatcg
      601 ctaataatat atcactattt tctacaattt gtttattaat aacactttgc ccagagctat
      661 tatttatata agtaggaatc gcattcgttt cccatcgaat tggcatcaaa attacttttg
      721 tattatctgt attttctata ttccatctat atatggtttc ttctacttca ttcctctttg
      781 ctgctatatc acctggtgat gctataaata cttttattag atttatggtt ttcatttatt
      841 gtacctttct ctttatgtct ctttaaagta atttttgtat aaaatttatt gacttttaca
      901 ctataggaca acttttatct atttagtaca aaaaggccca ctaaactaat tgattagtta
      961 ggagcctatt acaggtatat tttcaactcg cttttaagca aattctacta ccagcttttt
     1021 gcctaatgcc ctagcgatat catcaagttt atcgaaagat acgttatccc ctctctcaat
     1081 tcgagcaata gtagattgag ttgtatgtgc tttttgtgca agctccgctt gagttaggcc
     1141 tgcttcctcc cgtgcatgat aaagagcaac cgcagtttcg agttttcttc cttcacggtc
     1201 atatgcttct gcaaaatctt tgtctttttg cttttcttca atcaattttt taatcatcat
     1261 ttgtgaacca ctcctctcta agttcttttg catgtttaag ttcacttatt ggtgttttct
     1321 ccgttttttt agtaaatcca tgggtaatga cataccttcc attgaccaca tggaaataga
     1381 tagctctttg gatgtttgat cctaccttac ttcttaattc gtacaaatta gtgtctaatt
     1441 ttttcaccca tttcaaccgt tgagctacta gcatcccatt cttttctgtt tcttctattg
     1501 tacgtaaaag cttcgcacta tctttttttg gtaatgtttc caaccattct aaaaattcat
     1561 catgaccatt aggtctttta aatgtttcaa aagatatttt ttccataccc aattatatca
     1621 tatattgatt ttatattata gtatataaca taaaaattcg aat
//
LOCUS       DAZUMK010000234         1643 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000234 DAZUMK010000000
VERSION     DAZUMK010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.245"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..707
                     /locus_tag="ACG808_002903"
     CDS             <1..707
                     /locus_tag="ACG808_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS5048464.1"
                     /translation="ELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLKKVRFS
                     FEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEINKTKK
                     FVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYYVVRIE
                     EFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFSS
                     LTSDLPKISLRNWLEE"
     gene            complement(844..1260)
                     /locus_tag="ACG808_002904"
     CDS             complement(844..1260)
                     /locus_tag="ACG808_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048465.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1263..1547)
                     /locus_tag="ACG808_002905"
     CDS             complement(1263..1547)
                     /locus_tag="ACG808_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048466.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
BASE COUNT          518 a          247 c          283 g          595 t
ORIGIN      
        1 cggaattaac agtcaaatat caaaatgaat tgaatttagt accgctgaaa aatttcaatg
       61 ctaagcagat ggatttattt tttgcattat gcgctcgtat gaaagatcgt ggtttgaaaa
      121 aagttagatt ttctttcgag gaattaaaag agttaagtga ttataagatg actgctacaa
      181 aagcctttgt cggtgattta gaaaagctct ataaagatat gcttaatctt acttaccgta
      241 ctgaagatga ggattctatt cgctactttg ttttgtttac tggttttgaa atcaataaaa
      301 caaaaaagtt tgttgaagtc agtgttaatc cggacctaga atatattatt aatggtttaa
      361 cagctgaatt tagtagattt gaattatctg ctttcacgtc gttgcgttct acgtatgcaa
      421 aaactctatt ccgactgctt atgcagtacc gttctactgg ttattatgta gttcgtattg
      481 aagagtttag gagcttaatg gatatcccag aatactatca aatgggtaac attgatcaaa
      541 aagtattaaa tcctgcatta cgagaactaa aaaaatactt caatgatctt aaagtcacaa
      601 aaataaaagc aaaaaagggc aataaaatcg ccaaattaga atttactttt tcaagcctaa
      661 cgtcagattt gccaaagatt tctctgcgta actggttaga agaataaata aaaaagaact
      721 cgcccccctt acattaagca agtagacaag tcccttacaa ccgactaaga ttgctagtac
      781 attctagcat attgtatggg gcttctaaat aaaaaacaga taggaataac tcctatctgt
      841 tttttatcgt tttagaaaat ttgttgggtc gaaatttcta gaagtgactg tttgactggt
      901 ttctttttga tgattctttg gtcgatttcg atttgatgga tttagtttag cttcgatttt
      961 ttctttcact tcttgatata ctttagaatt tgtgccaata agaacgcctg tatcttttag
     1021 atataaatca gcaaattctg cgatgatttc agtcatgatg gaaatttctt ttcccatagc
     1081 atttagtttt gcctgattgc tggtagtggt cgtgttggta tcgggttcat aactcataca
     1141 taaaaagcga atggcttccg agtagttatt gatatctgga tttttttcga tgatggaatc
     1201 taaaacgtca acatgactag ggtacaaagt aacatgtttt tggacagaag tttttctcat
     1261 ggttatatca ttcctccgat aagcatttca atgatttgat tatttgtgta gacatactcg
     1321 cttaatattt gagtattttc tgtttggata gcaataaatt tatcgagcaa taaatcgaat
     1381 tggtcttttt tgtaagtcgt aagttgacgg tcaaaatcac tttcgattaa aacttttaga
     1441 tatttatttc gactccattt ttctcctgtt ttattcgtga gttcttggca acgtttgtct
     1501 attgttgcaa caaactttgg atcaatattc cgaactaaaa tgtccataaa aaatctcctt
     1561 ttgcaattat gtataattta tgtataccat acaaatacta tatttgtata gctgtttttt
     1621 gccaaaatgc tatacataag cta
//
LOCUS       DAZUMK010000235         1608 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000235 DAZUMK010000000
VERSION     DAZUMK010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.246"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            66..662
                     /locus_tag="ACG808_002906"
     CDS             66..662
                     /locus_tag="ACG808_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFS5048467.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(720..1202)
                     /locus_tag="ACG808_002907"
     CDS             complement(720..1202)
                     /locus_tag="ACG808_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048468.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1308..1505)
                     /locus_tag="ACG808_002908"
     CDS             complement(1308..1505)
                     /locus_tag="ACG808_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048469.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
BASE COUNT          543 a          335 c          249 g          481 t
ORIGIN      
        1 tgctatactt catttcgtaa tcaattaaaa aaatgcaaaa cgcaaatcaa aggagaaatt
       61 ttattatgac aacttttact gacactttga aaaaccgccg ttcaatctat catctaggac
      121 gtaatgtttc actttctaat gaagaattaa caactttgat caaagaagca attaaagaaa
      181 gcccaactgc tttcaatgct caatcaacac gcgcagttat ccttttcgga gatgcacatg
      241 aaaaactttg ggaaatcact gaagaagcac ttcgcccatt gactccagca gaagctttcc
      301 caaatacaca aaacaaatta gctggtttca aaaatggtta tggtacagta ttattcttca
      361 aagatactga cgtggtaaaa ggtctacaag aacaatttga attatatgca gacaacttcc
      421 cagattggtc agaacaatca aacggaatcg caacagctaa tacttgggta gcattagttg
      481 ataaaggatt gggcgcaaac ttgcaacatt acaacccagt tattgacgaa gcagttgcaa
      541 aagaatggaa tatcccttca aactggaaac tacgttcgca attagtattc ggttcaccag
      601 aaacaccggc tggggaaaaa gaatacatga acgatgccga tcgcttccgc gtatttggat
      661 aattttattt gaaataaaaa acagaggttg tgacaaacat cttgtcgcaa cctctgtttt
      721 tattctggat caatcataat atcaatctct gcctcatcgt ttaagcgttg ggacaaaaat
      781 ggaactaatt cttctaatga tgcagaaaat ttcagcatcg gtcctcgcca tccgtttcgg
      841 aaaaaattca tttttacgat tttagaacct tttggctgat ataatacaat tttattgatc
      901 tcagaaaaag gaacaccttt tttgtcaaag ctattcaaaa caagtccttc ttctgtgatt
      961 cctctactgt ccaacaaaaa actaataaaa atcattgctg acaagatccc ttttgccaat
     1021 tcatctgctg taggatgact gatgagtgta taccaaatga ctagtgacac taagaagggt
     1081 atcaccaagt agacccatcg atttcgcaca gtccgataaa taatttcttt tcgaaccagc
     1141 caaagaaata aaaacatcac aacaacgaat aaaaacaata gaaccgtaaa gaatgttacc
     1201 atgacaactc gcctcgcttt ttcttctcca gaaaatagga aaagaatact gatttacctt
     1261 ctcaaagaaa ttggacttgc agcacgccgc acgtccaatt gttttcttta ttcaaatgta
     1321 tcatattttg caacaaccat gcttgctttt tcatctaata attcttggtc ctctgcagca
     1381 cattgggcaa ctttgaccac ggcgcagttt gtcatttttg atacaacttc accaatgacc
     1441 gtttttgtaa aaccaattgg tttgcatgca tattgttctc ctaaattaat catttcttcc
     1501 gtcatcaaaa tcctcctaaa ctgctggaac ttttctccac attaccactg ctatttcttt
     1561 ggagatttaa ctcattgatt tttatttaca gttaaaaatc aaccttac
//
LOCUS       DAZUMK010000236         1594 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000236 DAZUMK010000000
VERSION     DAZUMK010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.247"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..266)
                     /locus_tag="ACG808_002909"
     CDS             complement(<1..266)
                     /locus_tag="ACG808_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048470.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISA"
     gene            552..791
                     /locus_tag="ACG808_002910"
     CDS             552..791
                     /locus_tag="ACG808_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048471.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(1153..1413)
                     /locus_tag="ACG808_002911"
     CDS             complement(1153..1413)
                     /locus_tag="ACG808_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048472.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
BASE COUNT          546 a          279 c          213 g          556 t
ORIGIN      
        1 attgcagaaa taaggattcc aataatagat ataaaatttg acatggaaac gtctataaca
       61 ttaaaagaat aaagtgttga agaaagtaaa ctgattgaaa taacccctcc gtaaaaaata
      121 gtctgtgaat atacccactt tttttcagaa gacgtacttc gtttggttct aaaacctatc
      181 cctgcatttc tactcttatc aaaaaaccaa atatttagaa gtaataatat tgctattatt
      241 aagataaaat tactgtaaaa atccaaaagc tatcctccta gcaactattt aaatacccaa
      301 aaaaattacc tctagataat atcataccta cgttttcact tataaatttt ttcataaaaa
      361 aagcgtttag aggcataatc gttaatacag atggtaacca atgtatctgg tttgaactgg
      421 gatatcaccc tctccatata tgtattacta agactaatag atactataaa agacttgata
      481 gttttgcatt gattttgcct aatactgagc ataggtgtat gctaaacgat actataagga
      541 gtgtgtacat aatgaaaaga attctgttta tcagtttatc aaatttattg ttacttactg
      601 cttgtgggac ttctgcatca aagaacatac cttcagatac gcaaatttcg aatagtatcg
      661 attcaacaat ttctacaaat gagaaaaatt ctccggtaga aacttccgat ataaaagttt
      721 ctacctctgc aaaaaacagg gtaaagaaac tgtcagcacg tctcctttat ttcaaacaaa
      781 ctctgaatta gcttcttcat cttcttcagc aaataatgat gatagtttcg cgtttactcc
      841 taactaaatg caacctttac aaatgcttta cgataacaag cctgagctaa atgatcctga
      901 tatcagtttt gcattttttg aaatgattgg atcggatgat ctttttaaaa ctactagttt
      961 gtctatccga aaataggaca gttctggcag tataggtttt tatcgagttt ctccaccagg
     1021 cctcgttaca gagacagatt ctctaggcga accttttgaa taaatcaaaa atcatgaaca
     1081 atcactgcca actaaataaa gtgccttcaa aagcttggtc ttctcgcttt tgaaggcact
     1141 ttatttaaat ctttatgaaa ctaaagtatg ttcatatttg ttgctatagt tatatagata
     1201 ttctgttgtt tccaactcat aaacgtgagt atattttgcc actacttttc cgcatttttc
     1261 taatatttca ttgcgatcga cgtcatgata ttcgtctacg cagatcacaa cacaattttc
     1321 taatttactg attacttctc caatcacttt ttgtttcaag ccaataggtt ggcaaatata
     1381 tttttttcct aaaatgatag tttcgttatt catatttcat ccatccttaa tgttttttca
     1441 ttatttaaaa tgataataac attaaaatat gattatgaaa atcaataaca cttgtgattt
     1501 tataaaaaaa taaaaaaacc gaaaaacaga aatgcccact gtctttccgg ctctttttat
     1561 tcttgatttc tcttcttttt tgtttcaata gacg
//
LOCUS       DAZUMK010000237         1591 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000237 DAZUMK010000000
VERSION     DAZUMK010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.248"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..613)
                     /locus_tag="ACG808_002912"
     CDS             complement(<1..613)
                     /locus_tag="ACG808_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFS5048473.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFL"
     gene            901..1554
                     /locus_tag="ACG808_002913"
     CDS             901..1554
                     /locus_tag="ACG808_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5048474.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT          573 a          223 c          296 g          499 t
ORIGIN      
        1 gcaggaagta ttgagttagt ttgtaaagtc tgcgatcttc aaggatcgta aatagctgat
       61 gggttgtccc atcaacacaa ataaaactca tggaaccaga aacagacttc attgatttga
      121 actcatccat acacaacacc gtcggaagga agttcatacg tggctggtaa tgcttcgttg
      181 cttcacgcaa gatccgtgtc accgtcccat ccgagacgaa gtacttctta gcgattagtt
      241 tacgtgattg attttcgtac agttctatga gaatcgcctg cttcagttcc tttgttagtt
      301 gatgatgttc atcaactaag tccgttttcg cagaaaaggt ctgcccacaa tcatagcata
      361 gaaaacgggt acgttttaat tctaaaatag tcaacttttc tttgactttc aatatctgag
      421 tacgtgtcgt ataggaaccg ttgcggataa tacgaatcga tccacagtgt tcacaatggg
      481 aaggtgagtc atccaatctc cctttaataa actgcgcttg aacgccttta tattcttttt
      541 caatcagcca ttcttcatca aaaaataaat gtttatctgt taatccgagt aattttctag
      601 tatgattatc catagagcga ctcctttggt agggtgatgt tttggtcgac taaattttac
      661 caaaagtgag tctctttttg agttgaaacg aagaaaaagt gttagtagaa aatcgccttc
      721 gattttccaa caacacaaaa tattatagaa ccctttattc taaaggattt caaggtgtct
      781 gtttgcattt attagaaaaa agaaaaagta ttggcaacag aaggtgtcac caacactaga
      841 tattatagag ccataatatg ctgtataata tctagtgtat aggcttaagt ggaggtatat
      901 atgaaaatat taactgttga agatgataat atgataagag agggaataag tgaatatctt
      961 tcagaatttg gatatactgt tattcaagct aaagatggaa gagaagcctt gtcaaaattt
     1021 aatagcgata taaatttggt tatcttagac attcagatac cttttataaa tggtttagaa
     1081 gtgttgaaag aaattagaaa gaaaagcaat ttaccaattc taatcttgac tgcatttagt
     1141 gatgaagaat ataaaattga tgcatttact aatttagtag acggatatgt agaaaagcca
     1201 ttttcattgc cggtcttaaa agctagaata gattctttaa ttaagaagaa ttatggacat
     1261 ttagagaagt ttgaatataa ggatctatcg gttaatttta atagctatac agctaaaata
     1321 aatggtgaag aaatagatgt aaatgcaaaa gaactagaag tattaaaatg tttattggat
     1381 aataatggac aagtgttaac aagaatgcaa attattgatt atgtatggaa agatagcgaa
     1441 gaaactcctt atgatcgagt aatagatgta tatataaaag aacttagaaa aaaattacaa
     1501 ttagactgta taactactat aagaaatgta ggatataaat tggagagaaa atgaaaaaat
     1561 taaagatatt tccaaaaatg ttcatacaaa t
//
LOCUS       DAZUMK010000238         1579 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000238 DAZUMK010000000
VERSION     DAZUMK010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.249"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(57..1535)
                     /locus_tag="ACG808_002914"
     CDS             complement(57..1535)
                     /locus_tag="ACG808_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5048475.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT          511 a          289 c          218 g          561 t
ORIGIN      
        1 gaaaaaaaca taaatcagct aataaatatg tgagacaaag ctgccatcca taaatactag
       61 ctgatttttt cagttaaatg tctatttttt tctctgatga tggtatttcc aaaatattcc
      121 cagtcatctg gagtcggtgg atttttccaa attatctgtt ctttattaaa tttttcaagt
      181 aaacaatcag ataaataaat attcgtacga gaagtgattc gactctcaat cacaatattg
      241 taatttttaa atcctttcac ttgagaaata atgtaatcgg tataagcaga tccttgcgaa
      301 aaatcaatac agacatgaat cggtgcttca aaatatgata ccggacaaat accgaccaat
      361 aaaaagagat aatcataaaa taagcgaatc atgaaacgtt catcttttcc caagaaatag
      421 cgtttattct cttgtatgac cttttggatt gctgtactga atattggata cgtctcgaag
      481 aaaaactgta gctgcttttt agacgtaaag ctagtcatct caaagtcgaa tatccaattt
      541 tttcgattga catgcatcat acccatacga agttcttttc gtatatatag tctttcttct
      601 tcagagattt caatcgaaaa tggtaattga tcaatcaatt ccactgacat tttttgacta
      661 atcatgtgga tatcatgaaa atgagcttcc gcaagtccaa atgcgccatc taattcaaat
      721 tcttcttccg tgatcaaaaa caacatgata taagaaatct catcaatgat ctgctcttta
      781 gggatatgaa gaaaagaaat atgtgataaa tccttgatga tcttcattcc ttctgagctc
      841 ttaaagtatt catctgaaaa gaaacgtttt ccgattggtt gattcccata ggccctcaac
      901 aaactaatag tggttagcaa attgaatttg attcgttttg ttgcagacag ttttaaattg
      961 aacagatatt caaaatattg cacgatactc tttgcttcat tcatgacgct ttctctaaat
     1021 ggcatagaaa aaccattaaa gaccccgaaa tataccccaa ataatacgtt ccgtatgttt
     1081 atttcttccc caactaatat gtttttcttt aactttattt gatgctgttc aagaaaagag
     1141 actaattctc ttctttttac ataggctctt gatatgctta aaaaatgatt ctctgcaaat
     1201 ttttcgatcg ttacttttga atccacgatc tccgttaaca gtcgagcaat ttttgatcgt
     1261 tgaaagtaga ttcttcttaa atttttgata tccgtcaaat tcagacgttc gacatggatc
     1321 tctgcttctt ctgaaatttg gatctctggc tttgttttga attcagtaaa ttctttattt
     1381 aattctatga tataatttct tgttttaaat ttagacaaac ctaatgtttc atataactta
     1441 ggcagcacta cataattctt tttctcaaat tctaaatatc tagctagcag cagtttttct
     1501 ctatcttcct cttctataag gtaatccaac ttcatatcct acacctcaaa aaatgcctca
     1561 taccaattac ttgatatgc
//
LOCUS       DAZUMK010000239         1538 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000239 DAZUMK010000000
VERSION     DAZUMK010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.250"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..1488
                     /locus_tag="ACG808_002915"
     CDS             22..1488
                     /locus_tag="ACG808_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFS5048476.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          422 a          289 c          326 g          501 t
ORIGIN      
        1 taaagaattg gggtatagat tatggaaaaa cagcaagaca agggcttctt cgggcagccg
       61 aagggattgt caactttgtt cttcactgag atgtgggaac gatttagtta ttacggtatg
      121 cgtgcattac ttatctatta tatgtacgat acagttgcca acgggggact tggattacca
      181 cgagcaacag cattagctat catgtcgatc tacggatctt tgatctacat gtctagtatc
      241 gtcggcggat gggtatctga ccgagtgtta ggagcaaaac gcactgtatt cttcggtgga
      301 gttttcatta tgcttggcca catcgtcttg gctttaccat ttggtgtaag tacattgttt
      361 gtttcgatgg cattgattat tatcggaacc gcctttttga aaccaaatgt ttctggcatg
      421 gtcggacatc tttattcaaa gactgacttg cgaagagatg caggattttc gattttttac
      481 atgggtgtca acttaggggc attattagca ccactaatcg ttggtacaat cggtcaagaa
      541 tataactatc atttagggtt ctctttagct gctattggga tgttctttgg attattacaa
      601 tacatcatcc aaggacgcaa gacattagac ggagtgggta caacaccgcc gaatcctttg
      661 acaactgaag aacgtaaagg attcatccgt aacttgatca tcgcacttgt tgttatcgct
      721 gcagtattcg gcggggcaca actaactggt catctgacaa ttgacttttt tgtcaataca
      781 atcagtgtat tggggattat cttaccgttg tattacttta tcaaaatgct gacttcaaaa
      841 gatgtaactg ctgaagaaaa accaagagta tgggcttata tcccattatt ccttgcagca
      901 atcattttct ggtcattaca agaacaaggt tcatctatct tagctttatt tgctagtgaa
      961 cgtacacaat ctacgctatt tggtgtaacg atccaagcaa gctggtatca atcattgaat
     1021 ccaattttca tctttattct gacaccagta ttcgtaacga tgtggacgaa acttggtgaa
     1081 cgtcaaccat ctacagtagt gaaattctct cttggacttt tatttgcagg attatcgtac
     1141 ttgctattga tgttgccggg tatgctttat ggtacaagtg gaaaagtaag tccattgtgg
     1201 ttgttgggaa gtttcttcat cgtggttatt gctgaattgt gtctttcacc agttggtttg
     1261 tctgtgacaa caaaattagc accacgagca tttgaatcac aaacaatcgc tatttggttc
     1321 ttagctgatg cttcttcaca agcaatcaat gcacaaattg cacgcttcta cacaccggga
     1381 acagaatctg cttacttcgg tattatcggt gcagtagcaa tcgtcggtgg tatcttgcta
     1441 tttactgtga aagaaccaat caaaaaacta atgggctcta ttcattaatc aatgaacata
     1501 aaaacaggaa ttgcttattt gcagcagttc ctgttttt
//
LOCUS       DAZUMK010000240         1530 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000240 DAZUMK010000000
VERSION     DAZUMK010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.251"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            284..1474
                     /locus_tag="ACG808_002916"
     CDS             284..1474
                     /locus_tag="ACG808_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HFS5048477.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
BASE COUNT          487 a          297 c          320 g          426 t
ORIGIN      
        1 aaaataaaag tgcaataaaa gtgcaatttt tgcacctaat catactctgt ttatatatga
       61 aaactagatt ctgaatccaa tatttggtta acgagaggcg ttcgaatttg aacgtctttt
      121 atatattact ttcaaataaa tatgaggtag aatactatct attttctttt taacccaatt
      181 gttataatgg agagtgtaaa atattttgtg taaatagaaa aaaggaagtc ccttctgtag
      241 aatagagtta ccacaacaca ttcacagaag agaggacttc cctatgaacg attttactac
      301 agaaattcta aagactctag cgaacaaagg cgatttgaat gaattattcc gtgtccattt
      361 ggagaaagca gtcaatacgc ttctcaaaac ggagttaacg gctttcctag attacgaaaa
      421 gtatgatcgc attggtttta acacgggtaa ttctcgtaac ggctcctatg accgtacggt
      481 caagaccgag tatggggaac ttcatctcca gattccgcgc gaccgcaatg gtgagttcaa
      541 gcaacagact gttcctgctt atagacggac gaatgacacg ttagaggaga ccgtcattca
      601 cctcttccga aaaggtatta ccatgtcgga aatcgcagac ttgattgaga aaatgtatgg
      661 gcattactac acgccccaaa ccatgtccaa tataacaaaa tcatttacag aagaggtaac
      721 ggcgtttaat gggcgggagc ttcatgaccg ttatgctgct atttatatgg acgcaacgta
      781 tattccgtta aagcggaaaa ccgtcgccaa ggaagctatt catatcgcag ttggcattcg
      841 cccggacgga tcaaaggaag tattgagcta tgcgattgca ccgactgaat ccatcacgat
      901 ttgggaggaa attttattgg accttcaaga gcgcggtttg aaaaatgtcc tcctgttcat
      961 cacggatggc ttaaagggga tggtaggagc gattagtcgg ttctatccca aagctcgttt
     1021 tcaacattgt tgtgtacacg tttcccgtaa tatcagtcat aaagtgcgtg tcgatgatcg
     1081 taaggaagtc tgtgatgatt ttaaaatggt gtatcaagcc tcatctaaag aggtggcgtt
     1141 ggaagcacgt ggtacttttg cggaaaaatg gaaaaccagc tatccaaaag tggttgaatc
     1201 gattctttcg aacgatcact tgctcacttt ctatgatttc cccttggcca tacgcaagag
     1261 tatttattct acgaacttga ttgaatcctt taataaacaa atcaagaaat acagccaccg
     1321 caaggaacag ttccaaaatg aagagtcgat ggaacgtttc ttagtctcgt cttttgatac
     1381 ttacaaccaa aaattcctag gtcgcagtca taaaggtttc caacaggccg aaggcgagct
     1441 cgaacaaatg ctaagccaac tgattgagaa ttagaaacaa tctgcagtag ggaagaatca
     1501 tttacacaaa attattgacg ctcccttata
//
LOCUS       DAZUMK010000241         1520 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000241 DAZUMK010000000
VERSION     DAZUMK010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.252"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..624
                     /locus_tag="ACG808_002917"
                     /pseudo
     CDS             1..624
                     /locus_tag="ACG808_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406632.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
BASE COUNT          521 a          276 c          242 g          481 t
ORIGIN      
        1 gaaatatctg ttttttgaat gtttgcataa gttaaaagat gagcgtgctt tagtattctt
       61 tagaatagct tcttatacag gggaacgaaa aggagaaata ctcggactga catgtagaga
      121 catagatttc gataatcaaa ctatctgctt caacaaaacg ttagtagaac tatccgaggg
      181 ctctctatgt attaatccta caaaaacaga caaatcaaag ggagtaattt caattgataa
      241 tggaacctta cgtattctaa aaaatggaaa cgttctgtaa cataaaagaa cctttctaga
      301 gggattcgga tagataatat agatgatata cctgtaatca gcactatcaa agggaataaa
      361 aaaagggact tcctttacaa aggctaccct aaccatgtca tggataaagt agcacggaag
      421 ttcccagata tgaagataat caaagtccat ggcttcagaa agactcatgc tacccttctc
      481 tttgaagcag gtgctcaagt aaaagatgta caagataggc taggacattc ttcttctaaa
      541 acaatcatgg acatttatat taaagtatca aaaaatcggc aaaatgaaac agtcgaatta
      601 tttgattctt acatgaacgg atgataagcg aggtcaaaaa cggagtcaga ggatattatg
      661 gggatacctt tttacctctt aatcctttaa tatcaatcct ttaagcttcg acttttgcaa
      721 tcttcccttg ttcgatatag acccaaatcg atctatcaat aattatcaaa ctgagtcata
      781 tagagacaaa aatttcaaaa acgacctatt aattggtcat cgaatatcat ttaaaatcaa
      841 tacgacggag tcaaacacgg agtcaaaaca agcccgctga gagtaaaaaa tatacagtcc
      901 cgtaggtaaa gtgccacagg cctgtatatt ttttatttga gattacctga aatgattcca
      961 tttacttttt atctcatttt ttcctactag ttagtatcaa atactctcct attttctcaa
     1021 taaactattt tgggttacat ggtatctgaa acagctaaaa ccttgtcatt acagcaaaaa
     1081 atcactttat ctctgtttca tttcagatac atttcactta agcggattcc aaaaaatcaa
     1141 aagaaaaagg aacccctagt tagcctatgc tttttattgg tttctctcga aacacggtca
     1201 tataacaacg gggtggggta aatgaatgaa aaaggtggag gaaacattat tcaatagctg
     1261 tcttaaatac ttcgttataa aatgtgaaca aataaaagac atctatccct attttctaca
     1321 cttccacttt actactatct ttgaatcgta caattttctt aactcttctc atttacctca
     1381 attcattttc aagtggaatc aactcatcat ctaacatttt acgccatgct cgcttcattt
     1441 cttgtgggta tttaaatggt ttcgcttctg gatgtttggc tttatatcct tcgtaatcct
     1501 tactgttgat aattgctttc
//
LOCUS       DAZUMK010000242         1503 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000242 DAZUMK010000000
VERSION     DAZUMK010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.253"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(606..962)
                     /locus_tag="ACG808_002918"
     CDS             complement(606..962)
                     /locus_tag="ACG808_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS5048478.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(976..1386)
                     /locus_tag="ACG808_002919"
     CDS             complement(976..1386)
                     /locus_tag="ACG808_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS5048479.1"
                     /translation="MIKVYTSPSCTSCRKAKAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
BASE COUNT          462 a          273 c          214 g          554 t
ORIGIN      
        1 tgtttgttgt cttgaattga atcaagacgc cctacacatt tggtttgtct tactttgttc
       61 agttttcaaa ggtctacttc atcaggagtg ttccccgatt cgtttttggc tgccgtagca
      121 acttttatat catatcagag agacaagtaa ttgtcaactc ttttttaaaa gtttttttga
      181 tttcgttttt cgaaatctta cagcaactca tttatcataa cttatcattt gttatttgtc
      241 aagaactttt ttgaagtttt tttcgataga atcaagcttc acaagtggtt cttttttaac
      301 aactcagtta tcttatcatg tcatttctaa catgtcaaca actattttat atgtttttta
      361 aaatactatt ttattgcttt gctacttaaa acaacagaaa tgaatatatc atgtaaattt
      421 acacatgtca atatattttt aactctatat ccccacctag atttgaattt tgaccacata
      481 aattgagcat gtctctatag tagaaaaata aagtcaaaaa aagaacacat aacacgcttg
      541 atgctaagtg ttcgactgtt acaactgtat gaaatgctca gtgacttgat taaaacagtt
      601 aaagtctatt tatttaagat aagctgttcc atcaactgct cattgtatcc aataaataga
      661 acttcatcga taataatcaa aggacgtctg aggagattgg tatgttgtgt gcataaaaaa
      721 atcagttgtt tttttcctac acaggaaagg tcaatcgcaa tgttgtgaaa agcctcagac
      781 caagtcgcaa atagctgttc tgcttgcact tcttcaaaac tgcataattc ttctgcttct
      841 tctgtactta gatcgcgttc ttctaagtca acaaattgat aaggcaattg atggtgttct
      901 aaccattgtt tcgccttcag acttgttaca cagtgcgctg atccatagac tttcacttcc
      961 acgaaggctc cctccctatg aatttaagat gttattgttt tgtaaataga tcagatttct
     1021 ggcaacacat cgaatttcac gagggacaaa tgaccggatc tcatcttcat taaatccgat
     1081 tagtattctt ttttcatcaa tcaaaatagg tcgtctcaat aatgaaggat attctttgaa
     1141 taagacgatc atttgcttta aagttaattc gttaaaatct atgcccagct tttcgtaaat
     1201 tttcgaccga gtggaaataa tatcttgaac tcctccttca gtcgctgcca agatcttttt
     1261 gatctcactt tctgttaacg gttcggcaaa aatattcttc tcttcaaatt caagttgatt
     1321 cgcttgcaac catgcttttg cttttctaca ggaagtacag ctaggcgaag tatatacttt
     1381 tatcattttc tcactccttt ttaattataa tccaattcta cagtaatttc ttttaaaata
     1441 ccaattatta gctaataaaa tataaaaaaa atcaaaataa ctgattattt acgtttgaat
     1501 aat
//
LOCUS       DAZUMK010000243         1480 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000243 DAZUMK010000000
VERSION     DAZUMK010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.254"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..727
                     /locus_tag="ACG808_002920"
     CDS             <1..727
                     /locus_tag="ACG808_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5048480.1"
                     /translation="IDQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMN
                     NYVFPDYYNDAQGIVLMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYL
                     ILFLIKASNMYVFLILSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVY
                     SFARKIGQALAGGVGGWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLIL
                     FFLYPLSKDKVLENCELLKQKHK"
     gene            737..>1480
                     /locus_tag="ACG808_002921"
     CDS             737..>1480
                     /locus_tag="ACG808_002921"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5048481.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVD"
BASE COUNT          522 a          162 c          277 g          519 t
ORIGIN      
        1 aattgaccaa tctcaataca aagtttcatt gttaaaatct ttaaaagggt tagttaaaaa
       61 tagggcatta ctttctataa ttggtgcggc tattttctta ctccttgctc aattattaat
      121 tatgagtatg aataattatg tatttcctga ttactataat gatgcacagg gaatagtttt
      181 aatgaatttt atcaatccta ttttggtttt aatagttgtt tcgcctttaa ctttgttttt
      241 aagtaaaaaa tatggaaaaa aagaactggc atcagtagga atgttctttt cagcaatagt
      301 atatttgatc ttatttttga taaaagctag taatatgtat gtattcttaa ttcttagttc
      361 ggttggctat atgggtttgg gtgtgtttaa tactgttatt tgggcaaata ttactgatgt
      421 tattgatgat caagaagtaa agtctgatca aagagaagat gggaccgtat atgcggtata
      481 ttcttttgct agaaaaatag gtcaagctct agctggaggc gtcggaggtt ggactctttc
      541 tattataggc tatgactctt tagcaaatgt acaaacggga gatgttttag aaaagttata
      601 taacgcttca acattaattc catcaatttg ttttctgatt gtaggattaa ttttattttt
      661 cctttatcca ttatctaaag ataaagtctt agaaaattgc gagcttttaa aacaaaaaca
      721 taaataggag agaaatatga ataattggat attttgtatt gattcagatg gctgtgttat
      781 ggatacgatg acttataaac acaatctctt tttcggacca attgctgcag agatttttga
      841 tgttcatcat agagaaaaat tcctacagga ttggaataat attaatttat attcagagac
      901 tcgtggaata aacagatttt taggtttagt taaaacatta gaaaaaaatg atgtaacatc
      961 gattaatcga ttaaagcact gggtatctct aacaaaagaa ttgtctaatc catctttgaa
     1021 gagggaaata gaatattctc agtcagagga tttattaaaa gctttaaaat ggtcggaaga
     1081 agtcaatcaa cagattagtc aagcaaaagg attagataaa ccctttttaa gtgctagaga
     1141 tactataaaa gctctgaaaa aatatgggaa gataattatt gtaagctcag ctaataagga
     1201 agctgttcaa gaagaatggg aaagacatga attattatca ttagttgatg aactgtgttg
     1261 tcaagataaa ggaaaaaaag aagatattat tcgaagtgta attgaaaatg gttgtgattt
     1321 ggataagata ttaatgattg gtgattctcc tggtgatcta gaagctgcaa ataaaaataa
     1381 agttttcttt tatcctattt tggtcaataa agaaaaagaa tcatgggaaa acttaagaac
     1441 tgatgtttta tttaaatgtt tatcgggaga ttatgtagat
//
LOCUS       DAZUMK010000244         1472 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000244 DAZUMK010000000
VERSION     DAZUMK010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.255"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(141..1433)
                     /locus_tag="ACG808_002922"
     CDS             complement(141..1433)
                     /locus_tag="ACG808_002922"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFS5048482.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT          400 a          329 c          264 g          479 t
ORIGIN      
        1 ttcggaaaaa gggactgtga cacggttttt gtcacagtcc cttattctga ataaacagtg
       61 ttcaagttat tattatcgta tagataaaca cttcgactcg ctctggtaag aacgtcctga
      121 ttcagtcgtc gtttttatcc ttagatttgc gcccagacgc tttctaagat gtttgtttgt
      181 gttctatcag gtccgacaga aaatgttgag atacgtacac caacaagctc tgagatacga
      241 cgtacatagt tacgtgcgtt ttctggcagt tctgcaagtg ttttgcatcc tgtaatgtct
      301 tctgaccagc ctggcaattc ttcatagatt ggtttgcagc ggttcaactc tttcaagctt
      361 gctggatagt ggtagattag ttcgccatct aattcataag ccgtacaaat ttttactgtt
      421 tccaatccac ttaatacatc gatcgagttt aaagataggt tggtaatacc tgaaactcgt
      481 ttagaatggc gcataactac agaatcaaac cagcctacac gacgcggacg accagttgtc
      541 gtaccgtatt ctcgtcctac ttcgcggatt tgttctcctg tttcatcaaa taattcagtt
      601 ggaaatggtc catcaccgac acgtgacgta taggctttgc agacgccgac aactttattg
      661 atttttgaag gccctacacc gcttccgatt gttaccccac cggctactgg gttagaagaa
      721 gtaacaaaag gataagttcc ctggtcgata tccagcatta caccttgtgc gccttcaaac
      781 aacacgcgtt tgcctgcatc caaagcatca ttcaagataa cggaagtatc tgtgacgtat
      841 tgtttgattt gttgtccgta ttcataatat tcttcaaaaa tatcgtcaaa atcaagtggt
      901 tctgaatcaa acatttttac aaattgacgg tttttgtcat ctagattcaa acgaagacgt
      961 tcttcaaaaa tttctttatc taataagtct gcaatacgga tacctacacg agctgcttta
     1021 tccatatagg caggtccgat tcctttaatc gttgtaccaa tcttattttc gcccttagca
     1081 tcttcttgta attggtccaa tttgatatga taaggcaaga ttacgtgcgc gcggtcagaa
     1141 atacgcaaat tacttgtttc tacacctttt tcatgtaaat aagctaattc tttaaccaat
     1201 gatttaggat tgactacaac gccgtttccg atcacactga tcttgtcttt ataaaaaatc
     1261 ccagaaggaa tcaaatgcag tttataagtc acaccgttaa acttaattgt gtgccctgca
     1321 ttgtcgccac cttggtaccg tgcaatcact tcagcatttt cactgagaaa gtctgtgatt
     1381 tttcctttcc cttcatcgcc ccattgcgta cctactacta ctacagatga catgtgaaca
     1441 cctcgttcta tattatttaa tcctgaacca tt
//
LOCUS       DAZUMK010000245         1467 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000245 DAZUMK010000000
VERSION     DAZUMK010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.256"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            236..>1467
                     /locus_tag="ACG808_002923"
     CDS             236..>1467
                     /locus_tag="ACG808_002923"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFS5048483.1"
                     /translation="MNKNGEWNAARRIFIILMDVLVLNVSIYLSFLLKFQGEVPVRNL
                     ETFQHSAIFISIIFIALNILLGAYVFYNRMISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVILISFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVAIDNYYENTKKLIDDMDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFD"
BASE COUNT          483 a          233 c          274 g          477 t
ORIGIN      
        1 tatgaatatt gtaacttgtg aataaggaat tagcaggaga atactagttc agcataaagg
       61 aagtgatggg acttctgaga caactttctg ttatgcagaa atacacatat aaacagacaa
      121 taaacgcttt tccccgtttc ttttaatttc ctttacaatt tggttataca ctaggttttt
      181 aaactttgct atggtaagat gatagaggca ttattttatg gttaggagtg tatatatgaa
      241 taaaaatggg gagtggaatg cggcacgcag aatattcatt attcttatgg atgtacttgt
      301 attaaatgta tcgatctatc ttagtttttt gctgaaattc cagggagaag taccagtgag
      361 aaacttagaa acctttcagc attcagcaat ttttatttcc atcatcttta ttgcgttgaa
      421 tattttgtta ggagcttatg tattctataa ccgaatgatc agtgatattg tcttcattac
      481 tgttattgga caagtactaa tagcattggg gatcatggta gttacttttg ctggccgatg
      541 gtttgcattt ccgcgtggcg tcattttgat ctcgtttatc ctaggtacga tattacttag
      601 tgtttaccgg atagtcgttt acaaactgta cttaagagtc agtcgtgata agaaagtcct
      661 tatcgtgggt ttggagaaag atgtcgcacc agctatccaa aattttcaat ctaagaaaaa
      721 caataaacat aaggttgaag cagtggcaat cgataattat tacgaaaata caaaaaaatt
      781 gatcgatgat atggatatcg tctatttggc ttctaaaatc ccagaagatg aaaaaatcaa
      841 catttatcaa ttagtgacta aaaaagagaa gaaattattt ttaaatacga cttttgagaa
      901 tctgacaatg ataaatccga atattatgaa ttttgaagat gaaagcatca ttgaagcttc
      961 tgggttcaga atccaaccag aatacgaact attcaagcgt ctctttgata tcatcgtttc
     1021 gcttatttta ctgatcctcg cctcaccttt tatgctaatc acagcgattt tggtaaaaac
     1081 aacttcacct ggaccaatca tctataaaca agtacgtatt acgaaaaatc aaaaggaatt
     1141 ttcaatctat aaattccgtt ccatgagtgc gacagctgaa gcaaaatcag gaccagtctt
     1201 ggctaaaagt aacgatgcac gtgtgacacc agtaggtaaa tttatccgtg ctgtacgttt
     1261 tgacgaatta cctcaaatct ttaatgtatt gaaaggcgat atgtcgattg tcggtccaag
     1321 gccagaaaga ccatttttcg ttgatcagtt caatgaagaa aatccttatt attacttaag
     1381 acacaatgta cgtgcgggaa ttaccggata tgcacaagta tacggaaaat atgtatcaga
     1441 ttataacagt aaactacgtt ttgattt
//
LOCUS       DAZUMK010000246         1448 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000246 DAZUMK010000000
VERSION     DAZUMK010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.257"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            677..763
                     /locus_tag="ACG808_002924"
     CDS             677..763
                     /locus_tag="ACG808_002924"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFS5048484.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(1065..1289)
                     /locus_tag="ACG808_002925"
     CDS             complement(1065..1289)
                     /locus_tag="ACG808_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048485.1"
                     /translation="MEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNMTPERKRS
                     LEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT          434 a          296 c          230 g          488 t
ORIGIN      
        1 cgttttttca tccattcctt tcttcccccc ttacaaataa ataataacct ttaacaacca
       61 actcgtaaat aggttggttg ttattataca aaaggatatt agtactcaga taaaaagttg
      121 ttagtactca gataaaaagt tgttagtact cagataaaaa gttgttagta ctcagataaa
      181 aagttgttag tacccttctg tatccgttgg gagagtaagg ctcaactgcc ccctaaaaag
      241 ggttctaaaa aatctttaaa aaataaataa aataaacgcg cgcgcgcgag caaaatttcc
      301 cgccaaaaaa tcacccactt tttaaaataa acaggttcgc gcatgatatt aaactgccaa
      361 tctagctccc tatggtcgct gtaagaagga gtgatcgcta cgctctcaca ttttacttgc
      421 agcagtcgcc tttaggctcc ttcgccaaaa ataattcatt agggactcct ttgtggcgtc
      481 ctgagagctc gtctaagccg ttctaagaca agattcgata aaaactatgt ccttcgttct
      541 aaagcgcctt acaggcggtc agggcaagcc ctaacaaccc caaggcaata aaaaacttag
      601 gcttattttt gcttttttat tgtataattg attgtcgcct atccgacagt cttgcgactg
      661 gaaggaggtg tgtttcgtgt tcactaactt tatttgctcc cttcttgttg ggcttttaag
      721 cgctttgttc agttactggt tgaataaccg aaacaagaag taaaacttaa ttaaggcgac
      781 tcagcccaca aaaaaagccc cagagaatcc gacctctctg gggctttcgt gtgtttcgtg
      841 ttcacttatt tgcaatcaaa ttataacaga aataaatatt tatttcaaaa atatcctaca
      901 aaagtctaaa atccgtaatt atttcttata tgcaacattt acaaataaag tcgctctgag
      961 agctcgtatt tttcgattta agacgtttta tttagttttt ggtgtatttc attggtccaa
     1021 gctaaaaaac tcttagaagc gattttagag ccatttaaac ccacttaatt atattctcca
     1081 tgaaattcgc tccatgcgtc aaaagctgcg tctgaaattc cgccgtcttc gttcactagt
     1141 tgcattaatt tttccaacga tcgttttctt tcgggtgtca tatttttata ttccaacaat
     1201 ttttcgtctg ttaaatcgaa cttgattgct tgaattgctt gctctaattt ttctaagttt
     1261 tgaaaatatt cgtaaggaaa tttttccatt ttttctttct ccagtctcac aatttttcta
     1321 gaaataaaat cacttctttg ccgtctatac tctcaaaaaa aatgtcgttt gaaaaactaa
     1381 ataaattttt gatcgtgcat ttttaaattt ggttatcgat tacacgtgca gcactccccc
     1441 cccgaatt
//
LOCUS       DAZUMK010000247         1424 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000247 DAZUMK010000000
VERSION     DAZUMK010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.258"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..1337)
                     /locus_tag="ACG808_002926"
     CDS             complement(150..1337)
                     /locus_tag="ACG808_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFS5048486.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DAZUMK010000248         1376 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000248 DAZUMK010000000
VERSION     DAZUMK010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.259"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(25..109)
                     /locus_tag="ACG808_002927"
     tRNA            complement(25..109)
                     /locus_tag="ACG808_002927"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(73..75),aa:Leu,seq:caa)
     gene            complement(127..198)
                     /locus_tag="ACG808_002928"
     tRNA            complement(127..198)
                     /locus_tag="ACG808_002928"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Gln,seq:ttg)
     gene            complement(208..280)
                     /locus_tag="ACG808_002929"
     tRNA            complement(208..280)
                     /locus_tag="ACG808_002929"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(245..247),aa:His,seq:gtg)
     gene            complement(342..426)
                     /locus_tag="ACG808_002930"
     tRNA            complement(342..426)
                     /locus_tag="ACG808_002930"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(390..392),aa:Leu,seq:caa)
     gene            complement(447..517)
                     /locus_tag="ACG808_002931"
     tRNA            complement(447..517)
                     /locus_tag="ACG808_002931"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(483..485),aa:Cys,seq:gca)
     gene            complement(563..634)
                     /locus_tag="ACG808_002932"
     tRNA            complement(563..634)
                     /locus_tag="ACG808_002932"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(600..602),aa:Gln,seq:ttg)
     gene            complement(646..718)
                     /locus_tag="ACG808_002933"
     tRNA            complement(646..718)
                     /locus_tag="ACG808_002933"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(683..685),aa:His,seq:gtg)
     gene            complement(721..794)
                     /locus_tag="ACG808_002934"
     tRNA            complement(721..794)
                     /locus_tag="ACG808_002934"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(760..762),aa:Trp,seq:cca)
     gene            complement(801..881)
                     /locus_tag="ACG808_002935"
     tRNA            complement(801..881)
                     /locus_tag="ACG808_002935"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(845..847),aa:Tyr,seq:gta)
     gene            complement(883..958)
                     /locus_tag="ACG808_002936"
     tRNA            complement(883..958)
                     /locus_tag="ACG808_002936"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(923..925),aa:Phe,seq:gaa)
     gene            complement(994..1066)
                     /locus_tag="ACG808_002937"
     tRNA            complement(994..1066)
                     /locus_tag="ACG808_002937"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1031..1033),aa:Asp,seq:gtc)
     gene            complement(1076..1148)
                     /locus_tag="ACG808_002938"
     tRNA            complement(1076..1148)
                     /locus_tag="ACG808_002938"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1113..1115),aa:Val,seq:tac)
     gene            complement(1164..1235)
                     /locus_tag="ACG808_002939"
     tRNA            complement(1164..1235)
                     /locus_tag="ACG808_002939"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1200..1202),aa:Glu,seq:ttc)
     gene            complement(1250..1339)
                     /locus_tag="ACG808_002940"
     tRNA            complement(1250..1339)
                     /locus_tag="ACG808_002940"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1303..1305),aa:Ser,seq:gga)
BASE COUNT          387 a          360 c          320 g          309 t
ORIGIN      
        1 agaaagttgc tatattgata attataccgg cggccggggt cgaagcggca cgtcctcaac
       61 ggacactgga ttttgagtcc agcgcgtctg ccaattccgc cacgccggca aatttaaaaa
      121 taataactgg ggtagctgga ttcgaaccaa cgcatgaggg agtcaaagtc ccttgcctta
      181 ccgcttggct ataccccaag aataaagggc gaatgatggg aatcgaaccc acgaatgccg
      241 gagccacaat ccggtgcgtt aaccacttcg ccacattcgc catcataaaa gatgttattt
      301 aatttctcaa taaaaaacta agatgaagac atcttagtca ataccggcgg ccggggtcga
      361 accggcacgc cctcaacggg cactggattt tgagtccagc gcgtctgcca attccgccac
      421 gccggcaagt tgatttatta gtactaaggc ggtaaccgga tttgaaccgg tgatgaaggt
      481 tttgcagacc tctgccttac cacttggcta taccgccatt atactattat caatctataa
      541 aataaagaat aaattataga aactggggta gctggattcg aaccaacgca tgagggagtc
      601 aaagtccctt gccttaccgc ttggctatac cccaagaata aaaagggcga atgatgggaa
      661 tcgaacccac gaatgccgga gccacaatcc ggtgcgttaa ccacttcgcc acattcgcca
      721 tggcaggggc agcaggaatt gaacccacac taacggtttt ggagaccgct gttctacctt
      781 taaactatgc ccctataaaa tggaggagag tggattcgaa ccactgaacc ctaaggaacg
      841 gatttacagt ccgtcgcgtt tagccacttc gctactcctc catggtggcg cgggacagaa
      901 tcgaactgcc gacacacgga gcttcaatcc gttgctctac caactgagct accgcgccat
      961 attttaaaat tattaattga ataacaagaa cgacggttcc gacgggaatc gaacccgcga
     1021 tctcctgcgt gacaggcagg catgttaacc cctacaccac ggaaccagtt cattatggag
     1081 gttaacggga tcgaaccgct gaccctctgc ttgtaaggca gatgctctcc cagctgagct
     1141 aaacctccaa cattcaatta aaatgacccg tacgggattc gaacccgtgt taccgccgtg
     1201 aaagggcggt gtcttaaccg cttgaccaac gggccaggta aatttgctac ggagagtaag
     1261 ggattcgaac ccttgagaca gtttggcacc atctacatga tttccaatca tgctccttcg
     1321 gcctctcgga caactctcca gagtgaaaga agctcaatac tctttctcaa aatgag
//
LOCUS       DAZUMK010000249         1364 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000249 DAZUMK010000000
VERSION     DAZUMK010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.260"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>1364
                     /locus_tag="ACG808_002941"
     CDS             82..>1364
                     /locus_tag="ACG808_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFS5048487.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          465 a          262 c          257 g          380 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taat
//
LOCUS       DAZUMK010000250         1359 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000250 DAZUMK010000000
VERSION     DAZUMK010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.261"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            313..855
                     /locus_tag="ACG808_002942"
     CDS             313..855
                     /locus_tag="ACG808_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048488.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            1011..>1359
                     /locus_tag="ACG808_002943"
     CDS             1011..>1359
                     /locus_tag="ACG808_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFS5048489.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          525 a          166 c          260 g          408 t
ORIGIN      
        1 ctgcgcaagg cttaccagcg agatagagat cgacgatttg ttgtttgaat tcttttgaat
       61 aggttcttcg ttgacgtctt ggcatgaaaa atcctccagt gtgtttttta ttattctaca
      121 caccttattt tttctgtcta gtctagtgta gccgattcaa gttcttcgtg ttctcttaga
      181 cataaaaatt cctcctggta tgttttcttc tagtctacac accttaattt ttctgtctag
      241 ttaattgtag cctatccagt ttggaaaaat aaaaaattat atttcatcaa cgtttcataa
      301 aaggtgtgta gaatgttatt gaaagtagct ataaaatgct attgtgtaaa aaataaagga
      361 ggaataattt atgaaaaaat agtaccaaga ggagcatgcc tattacttgc attatgtttg
      421 agcttaagcg cagttgggtg tcaaaaaagt aatgaaaaca ataaaataaa agaaggacaa
      481 tccataagtt ctgaagagga aggcatgtca attaatgcaa atggcaaaaa tgaaactttt
      541 aagccatcag attatacatt ggaggcaaag aaagaatatg tttatgaata tttagggctt
      601 aaatttaaac tctcagacaa atttagaaat tatatagctg ataaaaagat agcgatgtta
      661 gatgatcaaa gtcctataga taaagaacta aagtatgcta ttttgacatt tgagaaaatg
      721 acagaagaac aaaagaatgc agttatagaa aagatgggcg atggatataa aaattggcaa
      781 aatgagcttg aaagaattgg gactattggt atatttgaaa aaaatacatc agaagaaaaa
      841 aatctaaaat tataaaatgt gatactcata ctaaaatagg agtttcaagt gatggaaaat
      901 acgattgcta tttaagcaca aatagcggag ctgaaagtaa tcttcttgat gaattaaaaa
      961 gaactgagat tcaaattata gataaaaaag aacgtcctaa aaatggtttt gtgctatcag
     1021 aaaaaactga tttagaaaat acagaagcat ttaacaaaga atcggttaaa gatttacgta
     1081 aactaccaac aaaggacata aatggcaagg attttacaag taaagatttt gagaagtatg
     1141 atcttactat ggtaaatgta tttgcaactt ggtgtacagc ttgtgtaaag gaaattcctg
     1201 atttggttga ggttcagaat gaaatgaaaa gcaagggtgt aaatatcgtt ggagttgtta
     1261 cagatcctgt tgatgataac ggtgaaaaca aggaagcaat tgaaaagtca aagttaatac
     1321 atgaaaagac aaaggcttca tatcctttct taatgccag
//
LOCUS       DAZUMK010000251         1312 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000251 DAZUMK010000000
VERSION     DAZUMK010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.263"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            135..1220
                     /gene="xerS"
                     /locus_tag="ACG808_002944"
     CDS             135..1220
                     /gene="xerS"
                     /locus_tag="ACG808_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFS5048490.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
BASE COUNT          522 a          219 c          195 g          376 t
ORIGIN      
        1 ctatacctat ttatctgctt ttttgaaata aattaataaa tttcagagta taccaattga
       61 caaaacactc tataattggt atagtccaat tatagagata ctcctctccc ctacttatcg
      121 aaaggatgaa taacatgaaa aatacagaaa tatatcaaaa aatagataca atcatacttc
      181 atatgcccga ttatataaaa gattttgttc aagatcgtga ggataaagat caatcacctc
      241 gaactacttt agaatatcta aaaaattata agctctttta tgaatggctt ctgagtgagt
      301 ctatcgttcc cgatacttca atcacctcga taagacagtt aactgcctat gatctaaact
      361 tgtacaaatc tcatttgaaa cgtcgcacaa aggaaaatgt aaaaaaagag acaacaaaaa
      421 ctaagctcaa agataataat aacttaggtc tatctacttc tactatcaat agaaatatca
      481 cagcattaaa ggttctattt aaatatcttt cgaaaagttc caataatcct ttaggaaaac
      541 catacttaga aaataatcct atggatcaag tagcaactat cacagataaa actacgttag
      601 ctgcccgtgc aaacgcaatc gagaaaaaat tatttctaga cgaagataca caaaattatc
      661 tagattatat tgccaatgaa tataaaaaaa ctttgagcaa acgagctttg atctattatc
      721 aacgagatgt agaaagagac ctggcgatca atgcattgat tttaggatct ggattaagac
      781 tctccgaggt tgtaaatatt aatttagagg acctatcttt agaaaagaat aatgtggtag
      841 taactagaaa aggaaataag agagacgcag taaatatagc agcatttgca atggaatatt
      901 tagctaatta tctagctatt cggaaagaac gatacaaagt aacagaaaac gaaaaagctt
      961 tatttctagc aatttatcaa ggagaagcca agcgtatatc cggtattgca atcgaacgga
     1021 tggtagcaaa atattcaaaa ggattcagag tccaagtcag tccacataaa ttacgtcata
     1081 ctttggctac tagactctat caacagacaa attcattggt attaactgcc caacaattgg
     1141 gacatacaag tacaaataca acaactttat atactcatat agacaatgca gctacaattg
     1201 atgctttgaa cgctttgtaa atgtagtaaa aaatagtata ataatgaagc ctgtctctaa
     1261 aattagataa aagcccaact ttaaaggcaa gcttcattat catttttata tt
//
LOCUS       DAZUMK010000252         1298 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000252 DAZUMK010000000
VERSION     DAZUMK010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.264"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(53..787)
                     /locus_tag="ACG808_002945"
     CDS             complement(53..787)
                     /locus_tag="ACG808_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFS5048491.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1113..>1298
                     /locus_tag="ACG808_002946"
     CDS             1113..>1298
                     /locus_tag="ACG808_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFS5048492.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNN"
BASE COUNT          440 a          240 c          232 g          386 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaagaaacat caacacgaaa
      841 agcatcggtg cagtgtttcg tgtcggaagg atttctgcag tcttaacaaa tgtgaataaa
      901 aaaatgaatt ccattactta taagaaacca gaccaacagt aaaactccaa ctctttttaa
      961 aagttctcca caaatcacca agcttaacta taaatatacc aaaaaaaaaa cgtttttttt
     1021 ctcaaaaatt gtatcttaga aacagcatgt tttgaaactt tcagaaaagg tttgttagta
     1081 aaatgaaagc tatacgtaag gagtgttgtt tgatgaaatt ttttcaagag tttttcatta
     1141 ttattttctt ttctgtatta ggagaaggca tacggatttt tgctcattta cctattcctg
     1201 gaagtatctt aggtattctt tttttatttt tagcatttga aatgcatctt gttgatcctg
     1261 aaaaaatcgg agcaactggt gattttttat tgaataat
//
LOCUS       DAZUMK010000253         1297 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000253 DAZUMK010000000
VERSION     DAZUMK010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.265"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(162..1202)
                     /locus_tag="ACG808_002947"
     CDS             complement(162..1202)
                     /locus_tag="ACG808_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFS5048493.1"
                     /translation="MTDFNFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCSVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKLDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKIFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
BASE COUNT          381 a          206 c          182 g          528 t
ORIGIN      
        1 acgtaaaagg ctacgtttgt gatttttatc ataatatttt aacttataaa ttctattaaa
       61 atagctatta ttttgtaata ttcgtaatat aattagaata ataaaaaatt tttaatttta
      121 atgttacttc atgaagcaac aatcactttg tcagtaaagt ttcactctaa ccagtctttc
      181 gtaatagtgc ttggggagct ttcataattc aaacgttttt cacttgatac tttagcagct
      241 attggttttt cgataaacca attttttaca tagacaaaaa tcaggttctg gatattttcg
      301 actttttcgg tttttaattt ttgataggct tttaacagtg tcgtgtataa ttcttgttct
      361 gcattgacat aataactatc gagttcttca aaaactatcg aaataccact ttctatttca
      421 gcttttttct ttgcattatg aatagtctgg acggtttttt gagcttcact gtaacttgaa
      481 gaaaatattt ttatcaaatt taatactcgt tcagggacaa acgtagcaat gctgtcgttt
      541 gtcataattg tctcaaaatt gtctaacaac aattgatctt gtaggcggtc tttttcagtg
      601 tctatattgt gtctattagt gtctaatata ttattattaa gttctggtat gattttcata
      661 ccctctgatt tatcaacgtt ttggcacggt atgattttca taccctcttg agccccaagg
      721 gattcagcgt ctaatttttc gtcgagggtg ggtatgattt tcataccctc tgatttatca
      781 acgttttctc tattctctct atcaaatttt tcgagtaagt agatatcatt ttctgagact
      841 tttaattctg ctagatatag tctattagga ttatttttcc ctaactgttt gttaaatccc
      901 atctgctttt gtaataataa accaaaggat tccaactctt ttttgattgc tatcgctttt
      961 tgtttttggc agtttaatac gctacatagt tcatttgtcg tataagtaaa ataaacatta
     1021 ccttcttcat cgatttgccg tttgctgatt gcgatctcta atcttgcttt taaaagcata
     1081 taggcaatct tagttgaatc tttcattttt ttgtattcgt cagaaacaat aaagactttt
     1141 ggaaattgat aaaataattc gttgtaaact ctatccgcct taaaaaaatt aaaatcagtc
     1201 attgtagatc ctctactttc acgttgtttt atttatgtaa aattacctac tcttttcgaa
     1261 aatagcaaag ataaataaaa aatgcaaacg cattaaa
//
LOCUS       DAZUMK010000254         1285 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000254 DAZUMK010000000
VERSION     DAZUMK010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.266"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(14..1153)
                     /locus_tag="ACG808_002948"
     CDS             complement(14..1153)
                     /locus_tag="ACG808_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFS5048494.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT          335 a          281 c          239 g          430 t
ORIGIN      
        1 tattaaaaaa atcttacgct aagtaggata aaataattcc ttgatatttt tcgatcatat
       61 ccaaataatt atcgatttca acatactcat ctacttgatg aggcaaagta acgacaccag
      121 gcccaaagac gacaaagtcg aaggaatggt tagctttcgt aaattcggct gcatccgttg
      181 ttgctgccgc tccaactaaa ggtaatggct ggctgaattg ttgctgaatg caatgaatca
      241 aaggagaatc tggatcagct ttgacaggta ttttgttgta atcgatcatc aattccaaat
      301 gatagtctgt ctcttgattc aattcattga cgattgattg cagcaatgca atgattttat
      361 catttgggta ttctggaatc gatcgaatgt tcccttggag ctgcgcatgg cttgggatgc
      421 tgttgacttg attcccgcca ctgatcaatg taacattatg gattgttttt cctaggacag
      481 gattctcgat tgtttcagcg aggtgattca tttcagcatt tgctttcgta atgaattcat
      541 ttaggtgatt gattgcatta taaccttggt ctggcataga gctatgcgct tcttttccgt
      601 gcgaggtgac tgtatagttg attgatccca tgtgcgtata catcaaactg taattggttg
      661 gctctccgat gatcaaggca tctaagtcat ctacatagcc tgctttcgtg agctgttccc
      721 cacctagttc acccacttct tctccgacag tggctaataa tttgactgtt ccgttaaatg
      781 gttttcctga ctctttcagt tcgatcatgg caatcaccat tgctgccaag ccactcttca
      841 tatctgtagc accacgtccg tacaaacggt tcccatggat ttctgctgca aagggtgcat
      901 aggtccaaga cgattcatca ccagccgcca cgacatccat atgcccagat aggcctaaca
      961 cttttcctga ttggcccttt tggtaacgag caatcaaatt atctctacca tctctgtaag
     1021 aaacaatctc tcctgttatg tcatgctttg ccaaaagctt attcaaatag gctgctactt
     1081 caccttcgtt tccattcact gatttaattc ggataatctc ttgtaaaatc gcgatttttt
     1141 cttctttttt catctaatcc tctcccttgc catttctttt tattgtattt gagtgtgctc
     1201 gaattttcaa ataggagcaa ccaaagtaaa aatgaagaaa gagagccact ttatgaaaaa
     1261 tatgctgcta cttccctcct atttt
//
LOCUS       DAZUMK010000255         1240 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000255 DAZUMK010000000
VERSION     DAZUMK010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.267"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            110..982
                     /gene="scrK"
                     /locus_tag="ACG808_002949"
     CDS             110..982
                     /gene="scrK"
                     /locus_tag="ACG808_002949"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFS5048495.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          370 a          226 c          287 g          357 t
ORIGIN      
        1 aaaaatagta gttatttact tgtcatgtta ctaaataaaa aaataattta aatatgatat
       61 atagtcattt atttagtgat ttcattctaa aataaaggga gaaatgtcta tgctatacgg
      121 tgcaattgaa gcaggcggca caaaatttgt gtgtgccatt ggcgatgaag aaatgacgat
      181 caaggaaaga gtgagctttc cgacaacgac acctgaggaa acaatgcctt tagtcattga
      241 cttcttcaaa caataccaag cagatttagc agggattggg attggctcat ttgggcctat
      301 cgatatccat cgcgattccg ccacttatgg gtacattacc tcgaccccga aattggcatg
      361 gcaaaatttt gatttcatcg gtaccatgaa aaaagaattt ccgatcccga tttcttggac
      421 aacagatgtc aatgcggctg cctatggtga gtatgtcttc ggcagtggca aaggattgag
      481 cagtgtcgtt tactatacga tcggtacggg tgttggcggc ggtgccttac aagaagggcg
      541 ctttatcgaa gggtttagtc atccagaaat ggggcatatg ctcgtcgtcc ctcatccaaa
      601 agatgacttt gctggcagct gtccatttca tggcaactgt ttagaaggga tggccgctgg
      661 acccgccatc gaaaaacgct tagggaaaaa aggacaagag gttgcagaag atgatcctta
      721 ttgggaaatc gaagcctcct atattgctca atgtgcctac aatacaacct tgatgttttc
      781 tccagatgtg atcatttttg gcggtggtgt gatgaaacaa cgccatatgc tgcaaaacgt
      841 ccacgacgca tttgcaaaat tggtcaatgg gtatgtcgaa acacctgagt taagcaaata
      901 tatcgtgacc ccagcattag aagacaacgc tggaacgctc ggttgtttgg cattggctcg
      961 agaagcggtc ttacattcat aaacgaaaaa aggctcttcg acatttagtg ttggtagaga
     1021 aagatacaac tgaaaattta gtcataacca gttaggcaga gtgcttaact ggtttttgtt
     1081 ttacttggaa gatcaagcct tgaatgagat aaattcttgc tcgaaagtta tggaaatttc
     1141 gatagccgta ggccacttgt attgtgcaat agttttttac aaaaaaaagg gggtgcggat
     1201 atttttttgg acctttttaa caaatatatc tttgcttctt
//
LOCUS       DAZUMK010000256         1200 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000256 DAZUMK010000000
VERSION     DAZUMK010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.269"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..153)
                     /locus_tag="ACG808_002950"
     CDS             complement(<1..153)
                     /locus_tag="ACG808_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFS5048496.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
     gene            complement(475..696)
                     /locus_tag="ACG808_002951"
     CDS             complement(475..696)
                     /locus_tag="ACG808_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048497.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(794..1039)
                     /locus_tag="ACG808_002952"
     CDS             complement(794..1039)
                     /locus_tag="ACG808_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048498.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
BASE COUNT          368 a          233 c          149 g          450 t
ORIGIN      
        1 tacccaacga taaatcgtag tatgacaaac atttattcca cgatcatata acaattcctg
       61 aacttcacga tagcttagat tgtaacgcag gtagtaacca acagcgacaa taatgacgtc
      121 ttttttgaat tgtttgcctt taaaatgatt cattactctg tcctctctgt cttttttctc
      181 aattttacac taaaatagat tttttggaaa actttgcaac agaaccgttt ttactatata
      241 cttcttccca gaacttgtcc gtctccttca aattatctct acctctcgag ttcattgggt
      301 ggtttgccca tttattaaac atggaatcac ctttctcata ctcatatatt tatgtcccat
      361 ttcaattata tttatatatg cttaaataaa tttgtatcct gaaatttatt gtaatatgaa
      421 gctgtatttt acatatagta accaaagagt taataaaata aaaagagcga accattatag
      481 atcagctctt ttttcattgc tgtttttaat cttatctaga atcacttcaa atgtttcaaa
      541 ggtatttaca tctaactgac ataaccaaag caagtacttc ttttctttgt aagtccaatc
      601 aatattacgg taagaggaca ttagattatg gtaccttgct ctttgttcaa ctgaagccaa
      661 ttcccagcgt ttttcaaata ttttttcttc ttccattatt gatccctcgc aagacgctac
      721 ctcaaactta aataatgggc agcgaaagtc taattcctta gatctaagta tagcaaacaa
      781 atatgaaaaa aacttagaag gtagtaaaag ttcttccgtc ttttgctttg cttctattct
      841 taatgtgttt cgttatcggt gccctacaaa gattttcatt aattacacac tcatttattg
      901 ttgtgtattt tctgacattt ctctcttgat caattatttt aattccatgg gcatcctgat
      961 agttcatagg agaggagcgg ctatcagaaa tagttgcacc tatctcttga acttctttta
     1021 caacttctgg aaaatccatc catcctgctt cagctgtcaa aagtaataat ctataatatt
     1081 tatctttcct tttcaatcta agaaatcgtc accccattct ttttcttcta ttataaacta
     1141 tttaactttt agaattcaaa aaccgtttgt ctatttagag tttaccctaa atagacaaaa
//
LOCUS       DAZUMK010000257         1195 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000257 DAZUMK010000000
VERSION     DAZUMK010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.270"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1076
                     /locus_tag="ACG808_002953"
     CDS             <1..1076
                     /locus_tag="ACG808_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFS5048499.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DAZUMK010000258         1187 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000258 DAZUMK010000000
VERSION     DAZUMK010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.271"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(196..1011)
                     /gene="thiD"
                     /locus_tag="ACG808_002954"
     CDS             complement(196..1011)
                     /gene="thiD"
                     /locus_tag="ACG808_002954"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFS5048500.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT          309 a          258 c          198 g          422 t
ORIGIN      
        1 catgagaagc tgttttttga acttgttcct tgtgactcgg agctggacgg caggctcacg
       61 acctctttct taaacggtgt tcaaaagctt gcatctgcgg taataagtcc aaccaagtta
      121 aaaacataag aagctgtttt tacttggctg ttcttattac ttgatgcagg gcaacttttt
      181 cacaacttct ttcttttact ctaattcacc agctgcccaa tgattgatgg ggccatattt
      241 gtgccctaca tttatttctt cacgaattgc acggtttaca tagtcttttg ccaatcggat
      301 cgcttgttca actgtttttc ctttcgctaa ctcagctgca atacatgcag atagcgaatc
      361 gccagttccg ttgatccggt cggtaggata aaatggtgca tttaaccaaa atgattttcc
      421 tgattcgaac aatacgtagt cattgactgt ttcttgctgc gcatttttag catgatgtcc
      481 tttgatcata acgttttttg cacccatatc ttgcagttct tttgctgctt gcacaaaatc
      541 ttcttcagct gctaatgtcc gtccagtcag cttttctgct tcataaaaat taggtgtcag
      601 tacttcggcc aaagggacaa gcttttcttt caagctttgc aaagcagatt cttctagtaa
      661 aagatttcca tgtttcgtta cgattacggg atccacgact aatggtccaa aatcatattt
      721 ttggtaattt tttacaactg tctcaatcaa ctttgaatct gctagcatac ctgttttagc
      781 tgcacgtatt tgaaagtcct tcgcaatcag ttcaaattct ttgtcgataa aatctgttgg
      841 caatgtcaca cttgctccga ttccgtaaga attacctgca acacatgcgg tcatgacaga
      901 aacaccgtaa acatttcttg taaagaatgt atgcatatct gcttgcatcc ctgcgcttcc
      961 atcgctatct gatcctgcga tcgtcattgc ttgtacatac tgatcttcca tcaaatcgat
     1021 tcctccattt ttataaaaag atttttcata atactgttac tgtttctatc atatgttttt
     1081 caaagtatcc ctcagtaaat ataccactac cttttcttgg taatcaaatt tatttgaaaa
     1141 aacatttgca ataaatcaga aaatagtggt cgtttatttg tattcgc
//
LOCUS       DAZUMK010000259         1171 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000259 DAZUMK010000000
VERSION     DAZUMK010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.272"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            330..>1171
                     /locus_tag="ACG808_002955"
     CDS             330..>1171
                     /locus_tag="ACG808_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5048501.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILT"
BASE COUNT          436 a          138 c          195 g          402 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaaga attcttacca t
//
LOCUS       DAZUMK010000260         1167 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000260 DAZUMK010000000
VERSION     DAZUMK010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.273"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..700
                     /locus_tag="ACG808_002956"
     CDS             <1..700
                     /locus_tag="ACG808_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFS5048502.1"
                     /translation="KELQREIMPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITK
                     NFPSNIPNNYLEYYLYPDMVVEHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVL
                     NFNFGEHGQYIVDIAISLLNDDRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEP
                     ITLRKPISDFHKGLMEAQVAVEKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILD
                     EMIKVNKKYWPALK"
     gene            713..>1167
                     /locus_tag="ACG808_002957"
     CDS             713..>1167
                     /locus_tag="ACG808_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFS5048503.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFE"
BASE COUNT          436 a          173 c          241 g          317 t
ORIGIN      
        1 taaagaattg caacgagaaa ttatgccaga gattattgaa aaactaataa caaaagaaat
       61 gcaagtcgcc gattttaata taggagataa aacttggcaa aaaacctttc aaatgatgtc
      121 agtcatcacg aaaaattttc caagtaatat cccgaataat tacttagagt attacttata
      181 tcctgatatg gtcgttgagc atactgacaa agagtataca cgtgctaata tggtcatgga
      241 tggacgagag aaaaatacca aagaaatggc tgataaaata cgccgcggta ttgtggaaga
      301 agtattgaac tttaattttg gggagcatgg acaatatatc gtcgatatag ctatttcctt
      361 gttgaatgat gatcgtcgtc gctttatgct aatcgttcct aatcaagggg cgattccgaa
      421 tctacggtca gatgcagttg ttgaaatacc ggcatatgtt ggtgcaactg gtgtggagcc
      481 aattacacta agaaaaccaa tcagtgattt tcataaagga ttgatggagg cacaagttgc
      541 agtagagaag ttactagtag atgcttactt tgaaggttct tatcaaaaag ctttacaagc
      601 atttacattg aatcaaaccg taccaaatgc cagagtcgca aaaaaaatac tagatgaaat
      661 gatcaaagta aacaaaaaat attggccagc attaaagtag aggtgataaa aagtgcattt
      721 ctttaaaaca agaaacattc taaaagctgt agctagtggt caaattattc cattaacagc
      781 agtaaaagac gaggtctttt cttcaaaaat gataggagtt ggatacgcta taacgaatca
      841 tgatggatat atccatgcgc cagttaaagg aataatcgat aatatatttc ctacccgaca
      901 tgccataacg atcgaaagtg aatctggcag aaaaatactg atccatatgg gtttagatac
      961 agttgaactc gaaggaagtc cgtttcagat acgggttgaa agtaaccagg aggtaaaaga
     1021 agggacactg ttagctatta tagatttgaa gaagttggag caagcacaaa aagatacggt
     1081 gatcattgta gtttctccag gcatttttga aggaaattta ttaaaacaaa accaagaagt
     1141 aaagattaaa gatagttgtt ttgaatt
//
LOCUS       DAZUMK010000261         1164 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000261 DAZUMK010000000
VERSION     DAZUMK010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.274"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            49..603
                     /locus_tag="ACG808_002958"
     CDS             49..603
                     /locus_tag="ACG808_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5048504.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
     gene            659..>1164
                     /locus_tag="ACG808_002959"
     CDS             659..>1164
                     /locus_tag="ACG808_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048505.1"
                     /translation="MKITEFSEVFKTFSNIYNRIPEIEQNVVVDNDYYIENDFNKPRK
                     YDNLYNFIHNRTTINNNSNTRVLINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAI
                     NNGNLPSNKKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIF
                     ASIHDYIG"
BASE COUNT          476 a          118 c          212 g          358 t
ORIGIN      
        1 tatatatata atcatgaact cagaatattt ttaagaaggg aaacaatagt gagctataat
       61 gtacaaccgt taaggacaaa tgaagagata caagattttc agttttggct aagaagaaca
      121 agtaatccag aaagagatag ttttttattt ttgtttggaa tcaataatgg gttacgtatg
      181 tcagatatta taggattaaa agttggagac atacgaggta aaagtaagcc tattattgtt
      241 gaacataaaa caggtaagcg aaaaccgatt tttatagata atttgagaga agagatttta
      301 ttatatactg aaggtaaaga agaaaatgat tggttatttc caagtcggca acaaggtcga
      361 catataacaa gggatcgtgt ctatcagata tatgcagata tagcagaaaa attaggaaga
      421 gatgatatag gaacacatac attaagaaaa acttttgggt atcactacta caagaaaact
      481 agagacattg ctaccttaat gtttattttt aatcatagct cgcaagcaat tacaaaaagg
      541 tacattggta taactgaaga tgaaataggg gcatcgttac gtggttttaa attgggaggc
      601 taattgagat attaggatat attttttgtg tataatgaaa acttagaagg tgagattaat
      661 gaagataact gaattttcgg aagtatttaa aacgtttagt aacatttaca atagaatacc
      721 agagatagaa caaaatgttg tagttgataa tgattattat atagagaatg attttaataa
      781 accaagaaaa tatgacaacc tatacaattt tatacacaat agaacaacaa taaataacaa
      841 cagtaacact cgagttttga ttaattcagc atattttcat attaaatatt taaagaaata
      901 tgagtatgaa gaagttgtat tagattatgg gctaaataga cagtcatttt taatttatgc
      961 aataaataac ggaaatttgc caagcaataa aaagaacata attattgaaa catatgccta
     1021 tctggagggg ggatctaaaa aactaattaa gtcatgtaat atagagtatc aattatcagt
     1081 aggagaaatt aaatgtgttt tcgaagaaga tttagaactt gacttaaaaa aaatttttgc
     1141 cagcattcat gattatatag gttt
//
LOCUS       DAZUMK010000262         1123 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000262 DAZUMK010000000
VERSION     DAZUMK010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.275"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1123
                     /locus_tag="ACG808_002960"
     CDS             <1..>1123
                     /locus_tag="ACG808_002960"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HFS5048506.1"
                     /translation="DLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGSSRDKN
                     YLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGACTDGTE
                     VGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISSRSFGQ
                     NPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWDNSFGK
                     VYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGKLGFNG
                     LVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKGIITDD
                     RLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLI"
BASE COUNT          385 a          147 c          233 g          358 t
ORIGIN      
        1 gatttacgaa aaaaaccata taatttagat gaagatgcta tttcttggat tgaagagacg
       61 attgattcta tgactttgga agaaaaaata ggtcaattgt ttgttaatat gggatctagt
      121 cgtgataaaa attacttgag taatatggtc aatcagtacc atattggagc tgtacgatat
      181 aatccaggaa ctgctgatga ggtatatgat caaaactata tcttacaaac agaaagtaaa
      241 attcctctat taattgctgc taatacggaa gcaggtggta acggagcttg tacagatggt
      301 actgaagttg gtgtagaagt aaaaattggt gcaacaaatg atgcgaaata tgcttatgaa
      361 ttagggagag tttctgggat tgaagcatcg gctattggat gtaactggag ttttgctcct
      421 attgtagata ttaatagaaa ttggagaaat cctatcattt ctagtcgatc ttttggacaa
      481 aaccctgata aagttttaga acttagttta gcttatatga aaggtattca ggagagtgga
      541 attttgcctg cagctaaaca tttccctggt gacggtattg acgaacgaga tcagcatcta
      601 tcttttagta ttaatagttt aggtacggaa gaatgggata attcttttgg aaaagtatac
      661 tcaggcttaa tagaagctgg attaccttca attatggcag gtcatatcca tatgccagct
      721 tattcaaaac attttaatcc tgatctgaaa gatgaagatt tacttcctgc aactttatca
      781 aaagaattaa tcactgactt acttagagga aaactcggat ttaatggatt agtagttact
      841 gatgctagtc atatgttagg aatgactggg gcaatgaaac gatcagaatt attgcccact
      901 tctattgcag ctggatgtga tctatttcta ttttttaatg atccagatga agactttaat
      961 tatatgatgg aaggctatcg taaaggaatt atcactgatg atcggttatt agatgcctta
     1021 aaaagaattt tagggttaaa agctaaattg ggattacaca aaaaagcaaa agaacaaatt
     1081 ttattaccta agcaagaagc tctggaaaaa attggtttaa ttg
//
LOCUS       DAZUMK010000263         1099 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000263 DAZUMK010000000
VERSION     DAZUMK010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.276"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..979
                     /locus_tag="ACG808_002961"
     CDS             <1..979
                     /locus_tag="ACG808_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5048507.1"
                     /translation="PLITVLKTMIAGLGVILLAGVLIPNLPIITYILGGILVVSMQSF
                     LNALAFEYSNTGYRINFGFSRAGGSFSYAVTSFLLGQLLAIYSAAILPILVIVEAVIF
                     FVILHLLPDVKNQEVFPMTKEQDRSVSLRKKYPFLILLFLGFSFLFTFHTMINSFLAQ
                     IFENIHGGSKEVGIALMIAALCELPGMIFFEQLVKKKSSKFWLSVASISFAVRGFMML
                     LASSILMMEMTHLLQAFSFALFIPASAYLFNQFLADDDRVFGQTMIIIATTLGGVIGN
                     VLGGSLLQHFGVWEMLIFGTGFAVLGALLTILGIRKIPKTGNDSDSLVE"
BASE COUNT          290 a          193 c          235 g          381 t
ORIGIN      
        1 cccactgatc accgtgctga agacgatgat agcaggatta ggagtgattc tcttagctgg
       61 ggtattgatt ccgaatttgc cgatcattac ttatatcttg ggtgggattt tagtagtttc
      121 tatgcaatca tttctcaatg cgctggcatt tgagtattca aataccggct acaggatcaa
      181 ctttggcttt tcaagagctg gtggttcatt ttcttatgct gtaacttctt ttttactggg
      241 acagctgtta gcgatttatt ctgccgcgat tttgccaatc ttggttatag ttgaagcagt
      301 gatatttttc gttattttgc atctgctgcc ggatgtaaaa aatcaagaag tttttcctat
      361 gacaaaagaa caggatcgct cagtttcttt gagaaaaaaa tatcctttcc tgattttgtt
      421 atttttaggt ttcagttttc tttttacttt ccacaccatg atcaacagct ttttggcaca
      481 gatctttgaa aatatccacg gcggcagtaa agaagttggc atcgcattga tgatcgccgc
      541 tttatgcgaa ttaccgggaa tgatcttttt tgaacagctt gtcaaaaaga aatccagcaa
      601 attttggctg tctgttgcca gtatttcatt tgctgtcaga ggattcatga tgctgctggc
      661 cagttcgatc ctgatgatgg aaatgacgca tctcctacaa gccttcagtt tcgctttgtt
      721 tattccagct tcggcttatt tgttcaatca atttctggcg gatgatgatc gtgtatttgg
      781 acaaacgatg atcatcatcg ccacaactct tggtggggtg ataggaaatg tcttgggcgg
      841 cagtctgctg cagcatttcg gtgtatggga gatgctgatt ttcggtactg gttttgcggt
      901 gctaggtgct ttattaacta ttttgggcat tcgaaaaata ccgaagacag gaaacgatag
      961 tgattctctt gttgagtagc ataaaaatac ttagatacta gttttatata aataacatat
     1021 agtgattatt ggcgtaatta caaaaaacga gaataactta agtaagcatt tctaaataat
     1081 tgagaaacct catctaaaa
//
LOCUS       DAZUMK010000264         1092 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000264 DAZUMK010000000
VERSION     DAZUMK010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.277"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="ACG808_002962"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="ACG808_002962"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFS5048508.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGETKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          384 a          136 c          187 g          385 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaaact aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgatga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt ttcttgatgt gatatccttg tcttttttgt
     1081 aatgttttta ac
//
LOCUS       DAZUMK010000265         1037 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000265 DAZUMK010000000
VERSION     DAZUMK010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.278"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(20..91)
                     /locus_tag="ACG808_002963"
     tRNA            complement(20..91)
                     /locus_tag="ACG808_002963"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="ACG808_002964"
     tRNA            complement(113..200)
                     /locus_tag="ACG808_002964"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="ACG808_002965"
     tRNA            complement(225..298)
                     /locus_tag="ACG808_002965"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="ACG808_002966"
     tRNA            complement(312..382)
                     /locus_tag="ACG808_002966"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="ACG808_002967"
     tRNA            complement(384..459)
                     /locus_tag="ACG808_002967"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
     gene            complement(478..551)
                     /locus_tag="ACG808_002968"
     tRNA            complement(478..551)
                     /locus_tag="ACG808_002968"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(515..517),aa:Met,seq:cat)
     gene            complement(564..653)
                     /locus_tag="ACG808_002969"
     tRNA            complement(564..653)
                     /locus_tag="ACG808_002969"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(615..617),aa:Ser,seq:tga)
     gene            complement(677..750)
                     /locus_tag="ACG808_002970"
     tRNA            complement(677..750)
                     /locus_tag="ACG808_002970"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(714..716),aa:Ile,seq:cat)
     gene            complement(764..837)
                     /locus_tag="ACG808_002971"
     tRNA            complement(764..837)
                     /locus_tag="ACG808_002971"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(801..803),aa:Met,seq:cat)
     gene            complement(865..937)
                     /locus_tag="ACG808_002972"
     tRNA            complement(865..937)
                     /locus_tag="ACG808_002972"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(902..904),aa:Ala,seq:tgc)
     gene            complement(957..1030)
                     /locus_tag="ACG808_002973"
     tRNA            complement(957..1030)
                     /locus_tag="ACG808_002973"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(994..996),aa:Pro,seq:tgg)
BASE COUNT          281 a          285 c          246 g          225 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca gtgtttttag tttttaattg
      481 cgggagcagg atttgaacct acgaccttcg ggttatgagc ccgacgagct accagactgc
      541 tccatcccgc gataatatga aaaaggagga taagggattc gaacccttgc acggttttac
      601 ccgcctgacg gttttcaaga ccgttccctt cagccggact tgggtaatcc tccatatgaa
      661 acttacaacg ccacaatgga ccttgtagga ctcgaaccta cgaccggacg gttatgagcc
      721 gtctgctcta accaactgag ctaaaggtcc aggctcttaa aaatcgcggc ggaggggatc
      781 gaacccccga cctcccgggt atgaaccgga cgctctagcc agctgagcta caccgcgaaa
      841 aagatattca ttatttaaat gaaatggagc ctagcgggat cgaaccgctg acctcctgcg
      901 tgcaaagcag gcgctctccc agctgagcta aggccccgtg tttaaattta taattatcgg
      961 gaagacagga ttcgaacctg cgaccccttg gtcccaaacc aagtgctcta ccaagctgag
     1021 ctacttcccg tttaatt
//
LOCUS       DAZUMK010000266         1026 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000266 DAZUMK010000000
VERSION     DAZUMK010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.279"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(288..902)
                     /locus_tag="ACG808_002974"
     CDS             complement(288..902)
                     /locus_tag="ACG808_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS5048509.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          331 a          208 c          159 g          328 t
ORIGIN      
        1 caagaaagaa tccatatgga ttctttcttg tctctttttc cactactttt tatttttctg
       61 acactacata ttcttataac tcatttctca taactgtgaa acaacttata aaaataacaa
      121 actttaaaca aaacaagctt aaaatcaaag atgatccatc ttaaaataac taaaacaaaa
      181 aaagtagtcg ttgttaatcc tcattaataa tcgttttctt tttttcaaac gctccatttt
      241 tatgtggaac attatttttt taaggaactt tgtcaacatg tggataacta ttttccactt
      301 gtgtcctgga ctagctgttt tgctgtaaaa tacaaaactt gctgcagcaa aaagatcgtt
      361 gcaagagagg ctgttccgca gatcgctgga tctagatttg gcaaagtcaa gactgcatca
      421 tgtaaattgg cgtggttgtc caaaaccaaa tcagcaaatt caaataatcg caaaccactg
      481 ctatgttgcg atttgatact tcgtgacaaa tcaaatccag taacaatgat taatggatga
      541 ccattttctt tgatccactg agctagttcg atgattgccg ggtttcttcc ttcattcgaa
      601 agaagaagaa aaatgtcttc tgatttgaca gaaagctgat caataattgc ctgtcctgca
      661 ccttctagcg tttcatagac acctccggct ggattttcaa tcaatatact tggtacaaga
      721 tccggggttt gatcccaaaa ttcaaatgct acaccactta gtgtgcggct agcatatatc
      781 tgcaatctac caccttgatt aatttgtttc gcgatccaag tgccagcttg aagcatcagc
      841 ggctcttcta tccttaattt ctcttccaat tggtgataat agtcaaaata acataaactc
      901 atttatctct cccgcctagc tatacttgac atgaatagaa acagctgcct aatctaagga
      961 agtacataaa aaaacagtag ttagacagta acacatgtgg tctttgctgt ttcaaacaca
     1021 aaaaag
//
LOCUS       DAZUMK010000267         1008 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000267 DAZUMK010000000
VERSION     DAZUMK010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.280"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..599
                     /locus_tag="ACG808_002975"
                     /pseudo
     CDS             1..599
                     /locus_tag="ACG808_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          371 a          167 c          162 g          308 t
ORIGIN      
        1 ttagagtttc atccatcgat caaaatgaac aacgtcaaat tgaagaaatg aaaaagtttg
       61 gcgctgaaaa aatttttatc gaaaaacaat ctggagcgac gattagtcat cggccgattt
      121 tccaggaagc tttagatttt gtcagagagc aagatatttt tattgtagaa gccattgatc
      181 gacttggacg caattatgac gagattatcg attcagtgaa ttatctaaag aagaagaatg
      241 tgcgacttat tatcaccagt ttaccgatca tggctgaagc aattggaaat cctctcctag
      301 ataaattcat caaagactta atcatttaaa tctcagccat gattgctgaa caagaacgta
      361 ctgaatcgaa acgacgccag gcacaaggaa ttaaaatcgc taaagcaaac ggcgtctata
      421 aaggtcgtcc taagttatac agtgcagaca ccaaagatcc tcaacgtcgc cttgtctata
      481 ggagtatcgt tcaggatctt gagaatgggg ttgctatctc aaagattgca acagactaca
      541 atgtgactag gcaaacaatt taccgaataa aaaaagaaat cgaccaacta attgtatgat
      601 ttcttttttg aatcacgtaa ttattaatta gctttgtcat caatacagac aatcatttta
      661 gattcatcta gctaagttga ttacatttat gttaaaaaga atttagttaa cataaatata
      721 attgatgcat aaatcgtcct tatttaaagg catttaacat tgacaactta gtataatgta
      781 tcttatgtaa actaataaga tttgaatttt attttataca cactcgataa tagatcaatt
      841 ttatggtccc ccgcatttac gattgcacaa gagaaacata aagtcgacct attagcattt
      901 cttttcaatt caaacattaa aaaagtttat acctctgcta ctaaaaaatg aaattatcta
      961 tttaaaattt aaaacggtaa atctgattca tccaaattgg aaaatgga
//
LOCUS       DAZUMK010000268          890 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000268 DAZUMK010000000
VERSION     DAZUMK010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.282"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..888
                     /locus_tag="ACG808_002976"
     CDS             <1..888
                     /locus_tag="ACG808_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFS5048510.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          386 a          116 c          144 g          244 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DAZUMK010000269          883 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000269 DAZUMK010000000
VERSION     DAZUMK010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.283"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..662
                     /locus_tag="ACG808_002977"
     CDS             <1..662
                     /locus_tag="ACG808_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFS5048511.1"
                     /translation="YENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDTIRSEKNYS
                     IIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEIGK
                     TKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMHITIYNYIS
                     KRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNI
                     S"
     gene            679..>883
                     /locus_tag="ACG808_002978"
     CDS             679..>883
                     /locus_tag="ACG808_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048512.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESIS"
BASE COUNT          324 a           96 c          163 g          300 t
ORIGIN      
        1 tttatgaaaa taaaatactg gaatatgtta aaaatggaga tattcgaaat ttagaaaaca
       61 tggtttttaa tctgagtaat ggaataattc caagtgttag tggagatacg attcggtctg
      121 aaaaaaatta ttctatcatt gtttttgaaa agttagcaca aacgtctata acattaggaa
      181 tggatataat agaagcgtat caaagtagag atgctttgat acaagaaaac gaattagctg
      241 tatcgttacc tgaggtctta aaagtgaggg attctgggat tgtttattat actaaggaaa
      301 tcggaaaaac taagatagag catctttctc ccttaatttc ttctgtcgtt caattcatcg
      361 gcttaaatat ctataaaaga attactgtaa aggaaatagc taattatttt tctgtaagtg
      421 aaactaaatt aagagaaagc tttaagaatg aaatgcatat tacgatttat aattatatct
      481 ctaagcgaaa aatttctacg gctaaaataa tgttgaaatc aaaccatact atcagtgaag
      541 tttctttagg tttaggattt tcagattctt cacacttttc gagagtattc aaaaagtacg
      601 caggtgtatc tcctaagcaa tatcaattag gtttagtgga caatttcaac aacattagct
      661 gagaggtttt attttattat gagactatat catttacgta gtttggagga gttgaggaaa
      721 ttggatttta acaaaaaatt tttttgggaa atttctgatt tgagttgttt cgtagaatct
      781 gagcgtctaa gtaatggtaa taatagtgtg ttaaatgaat atgacaaagt agtaattaat
      841 aaagtaaaaa gtttatgtga agaagctaga gaatctatca gtc
//
LOCUS       DAZUMK010000270          851 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000270 DAZUMK010000000
VERSION     DAZUMK010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.284"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..669
                     /locus_tag="ACG808_002979"
     CDS             <1..669
                     /locus_tag="ACG808_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS5048513.1"
                     /translation="GYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVFES
                     SISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIATSH
                     STDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSSLA
                     KLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHTIS
                     EIKE"
BASE COUNT          281 a          151 c          155 g          264 t
ORIGIN      
        1 ggttataatg gatttaaaga ttttaaaatg gccatgttga tccaagaaaa tgatctatct
       61 gctatttcta tccacgaaaa tattcagaaa acagatactg agctcactat ggcgcaaaag
      121 gtgttcgagt ctagtatttc aactttaaat gacacaaaaa agttgttaca acaaaaggat
      181 ctaaaaaaag cggctactat tatcaatgaa tctaaacgtg tctatttttt cggtgttggc
      241 ggttcagaga ttgtagcaac tgacgcttat cataaatttt tgcgttcccc tatcgcaact
      301 tctcacagca cggattacca tattcaattg atggaagctt ctttattgac ggaaaaagac
      361 tgtgctgttt tgatttctca cacagggcaa tcaaaagaaa caatccatat tgctgaaacc
      421 gtaagaaaaa ctggagcaaa aacaatcgtt attactagcc aagctaattc ttcccttgct
      481 aaacttggcg atgtcgtttt tatttctatt tctgaagaaa cagaattccg atctgaagct
      541 ttagcttccc gtatttctca actaagcatt ttggactctc tatatgtgat tttgatgttt
      601 tacaacgaaa aagaatcacg tcgttctatc gctaaagttc gacatactat ttctgaaatc
      661 aaagaatagc accataacag aaaaagcgaa aaatccataa aacaaaaaaa gccactacca
      721 aatatttggt agtggctttt ttggttctgc aagggtggta cagaacatca atagtcggtg
      781 ggttagaacg tatgtcatcg catctttgga gaaggttgta ttggtattat tcgagttact
      841 tgttagatgc g
//
LOCUS       DAZUMK010000271          847 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000271 DAZUMK010000000
VERSION     DAZUMK010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.285"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>847)
                     /locus_tag="ACG808_002980"
     CDS             complement(<1..>847)
                     /locus_tag="ACG808_002980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS5048514.1"
                     /translation="VVGRIAGLKCKVKKVIYQAHGFHFYSGAPKINWFIYYPMERWLA
                     HYTDVLITINHEDYTLAQKLRLRKGGKAYYIPGVGIDTNRFMSSTSIRNFRESICKEL
                     NIPDTAKIILSVGELNENKNHLSVIHALQKIPLHTDIYYIIAGVGEMRETLQNIANEL
                     GLHQRVFLLGYRSDIPKLLAAANIYILPSLREGLNASLMEAMASGLPCIVGNIRGNVD
                     LIEDGKGGFLINPKDEEEIAGAINRLADNKLLCESMGAHNLDKIKDFDLSVVINQINN
                     VYHEIV"
BASE COUNT          266 a          165 c          111 g          305 t
ORIGIN      
        1 ttacaatttc atggtaaaca ttatttattt ggtttatcac aactgataaa tcaaaatctt
       61 ttatcttgtc taaattatgt gcccccatag attcacacaa taatttgtta tcagccaatc
      121 tatttatcgc accagcaatt tcttcttcgt ctttaggatt gattagaaat ccacccttac
      181 catcttctat aagatctaca tttcctctta tattaccgac aatacatggt aaaccactag
      241 ccatcgcttc cattaaagag gcgttgaggc cttcccttaa tgagggtaga atatatatgt
      301 ttgcagcagc taataatttg ggtatatcac ttctatatcc tagtaaaaaa actcgctgat
      361 gtaatcctaa ttcattagca atattttgta gtgtctccct catttctcct accccagcga
      421 taatataata gatatctgta tgcagcggaa ttttttgtaa tgcatgtatt actgataaat
      481 ggtttttatt ctcatttagc tctcctacag ataaaatgat ttttgctgta tcaggtatat
      541 taagttcttt acatatactt tctctaaaat tccttattga tgtggaagac ataaacctat
      601 ttgtatctat acccacacct ggaatataat aagcttttcc acctttccta agacgaagtt
      661 tttgagctaa agtataatcc tcatgattta ttgtaatcaa aacatctgta taatgagcta
      721 gccaacgttc cataggataa tatataaacc aatttatttt aggagcacct gaataaaaat
      781 gaaatccatg tgcctggtaa attacttttt tgactttaca tttaagacca gcaatccgtc
      841 cgacaac
//
LOCUS       DAZUMK010000272          843 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000272 DAZUMK010000000
VERSION     DAZUMK010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.286"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>843
                     /locus_tag="ACG808_002981"
     CDS             <1..>843
                     /locus_tag="ACG808_002981"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS5048515.1"
                     /translation="LNIRGLSYLPLKKFSRKEIDRAVHELNPDIIHAHDFNASVRASR
                     YKKQIILSHLHNNPLWLPKLDIRTIIYSLCSSRFNSVIGVSNSVKEEFRYKQLIKDKF
                     VLLPNTVNTERIIKMGNEFVEDDIDILYVGRMAEPKNPIGAINVLENVVKKYSKHLRV
                     VMIGEGPLFDEVKMQIQKRNLTDVIELKGFKSNPYKYMKKSKVLLMPSLWEGFGLVSV
                     EAMLLETPVVAYNVGGLKDIIDETNGFLCGSEVELSNSLMKLLNSEELRKKMGENAAI
                     SAKK"
BASE COUNT          319 a           98 c          160 g          266 t
ORIGIN      
        1 tttaaatatt agaggtttaa gttacttacc attaaaaaaa ttttcgagaa aagaaattga
       61 cagagccgta catgaattaa acccagatat aattcatgct catgatttta atgctagtgt
      121 aagagcaagt agatataaga agcaaatcat cctttcccat ttgcacaata atcccttgtg
      181 gttacctaaa ctagacataa gaactattat atattcttta tgttcgtcta ggtttaattc
      241 agtaattggt gtttcgaatt ccgttaagga agaatttcgt tacaaacaat taataaaaga
      301 taaatttgtg ttgttaccta acactgtaaa tactgaaaga attataaaaa tggggaatga
      361 atttgttgaa gatgatattg atattttata tgtaggacgc atggcagaac ctaaaaatcc
      421 aataggtgca attaatgttt tagaaaatgt agtaaaaaaa tattcaaaac atttacgtgt
      481 cgtgatgatt ggtgaaggac cattatttga tgaagttaag atgcaaatac aaaaaagaaa
      541 cttaacagat gttattgagt taaagggatt caaaagtaat ccctataagt acatgaagaa
      601 atctaaagtt ctattaatgc catctctttg ggaagggttt ggcctagtat cagtagaggc
      661 aatgttgtta gagacgcctg ttgtagctta taacgtagga ggattgaagg atattatcga
      721 tgagacaaat ggatttctgt gtggtagtga agttgaatta tctaattcac taatgaagct
      781 attaaattca gaagaactta gaaaaaaaat gggagaaaat gctgctatta gtgcaaagaa
      841 att
//
LOCUS       DAZUMK010000273          841 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000273 DAZUMK010000000
VERSION     DAZUMK010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.287"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            167..361
                     /locus_tag="ACG808_002982"
     CDS             167..361
                     /locus_tag="ACG808_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048516.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            630..>841
                     /locus_tag="ACG808_002983"
     CDS             630..>841
                     /locus_tag="ACG808_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048517.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFA"
BASE COUNT          349 a          106 c          135 g          251 t
ORIGIN      
        1 aagaacaata gccttttatt atatatatta ttcaaattag ttatatcgag tgaaaaaaac
       61 tttcaaattg attcttttta tttatcataa agatatagat acataatcat tttcacttac
      121 atatatttga atattaacta aaaacagcaa gaaagaagag gatcaaatgt attatgtaga
      181 agtaaaaaca aaaggcgtca aaaacaaaca atatgtgaaa ggtatcagta acgaatatcc
      241 tttacttggt tcatggaaag aagctgctcc tttttccaaa ccatgtgcca taaaaataaa
      301 gaacgagcta gaaaaagaac tgacttgtgg taaagctgtt gtagatatca tcgaaaagta
      361 aatccaatgt atagaaagga agatcacaca tgaaaagcga cgtacaaatt gctaagatta
      421 tagaattaga agaagaagac gcagtgttga caattgaaaa aaaataaatg tttgtatacc
      481 atatgattat ttcaatatgt atcctggaat tggagatctc gtagagctat atcaagatga
      541 atcaaatata cttgtcattc atcatgaaat atagagactt ataaaattgt ttatttttat
      601 aatgtatcag tatagaaagg aaataaaaca tggattatac tgttgaattt gacagtgaac
      661 tacttaagca atttccacaa gaagaacatt tcatgtataa agaagcaatt tcccacaaag
      721 tgacagagta tttggagcat aatttttttc gtataaagcc agtgcgtact cgttcggcaa
      781 aaggaatata cgagatgaaa gtcaaaatta ataaaaaaaa ttatcgcata gcatttgctt
      841 t
//
LOCUS       DAZUMK010000274          836 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000274 DAZUMK010000000
VERSION     DAZUMK010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.288"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>836)
                     /locus_tag="ACG808_002984"
     rRNA            complement(<1..>836)
                     /locus_tag="ACG808_002984"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          183 a          261 c          188 g          204 t
ORIGIN      
        1 tctctcaggg atcttacttt cttaaagaaa tgggaaatct catcttgagg tgggcttcac
       61 acttagatgc tttcagcgtt tatcccttcc ctacatagct acccagcaat gcccttggca
      121 gaacaactgg tacaccagcg gtaagtccat cccggtcctc tcgtactaag gacagctcct
      181 ctcaaatttc caacgcccgc gacggatagg gaccgaactg tctcacgacg ttctgaaccc
      241 agctcgcgtg ccgctttaat gggcgaacag cccaaccctt gggaccgact acagccccag
      301 gatgcgacga gccgacatcg aggtgccaaa cctccccgtc gatgtggact cttgggggag
      361 ataagcctgt tatccccagg gtagctttta tccgttgagc gatggccctt ccatgcggaa
      421 ccaccggatc actaagcccg actttcgtcc ctgctcgact tgttggtctc gcagtcaagc
      481 tcccttctgc ctttacactc ttcgaatgat ttccaaccat tctgagggaa cctttgggcg
      541 cctccgttac cttttaggag gcgaccgccc cagtcaaact gcccatctga cactgtctcc
      601 caccacgatt agtggtgcgg gttagagtgg ccataacgca ggggtagtat cccaccagcg
      661 cctccatcga aactagcgtt ccgatttcta cggctcctac ctatcctgta catgcggtac
      721 agacactcaa tatcaaacta cagtaaagct ccatggggtc tttccgtcct gtcgcgggta
      781 acctgcatct tcacaggtac taaaatttca ccgagtctct cgttgagaca gtgccc
//
LOCUS       DAZUMK010000275          831 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000275 DAZUMK010000000
VERSION     DAZUMK010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.289"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(200..703)
                     /locus_tag="ACG808_002985"
     CDS             complement(200..703)
                     /locus_tag="ACG808_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFS5048518.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
BASE COUNT          328 a          127 c          144 g          232 t
ORIGIN      
        1 agcctttaag ctaaaaaaaa accctttttg ttaaataagg ggaatatagc ctgtcagttt
       61 caaactgcaa gtcgtcctgc tgcaataata tagtagatta gagatgaaaa tagatttttt
      121 taaaccaatg actgaataaa aagaccgtca tagatacaca ttggaatgtg tctattacgg
      181 tcttttttta cgaacgattt tattgaagac ggacagttcg ggtcaaaaat aaaaaagcta
      241 cgatcagaaa taagaataaa atgtatccca aaataatggc tagacggtct aacttatttg
      301 tcgatagatg tttgtttaga agttgatagg cccataaacc tatcagtcct cctgttgtat
      361 tagttatcaa atcagtgatg tccgctacgc caataccgaa taggaattga atcaattctg
      421 ccaagaagct tacaaggaaa ataatcccta attttttaga aaaagaaagt ctcttgaaat
      481 tgacattcaa aagcacccca aaaggaaaaa agacaatcaa gttataagtg gtctcacgta
      541 ataccgtcgt attttcaaaa gaaaaagtag aaaaagggac gagattgaca ttgctgtaag
      601 aataagtgag aaattgtgga agattaaatg atagcttgaa taaaatcaac cataatagaa
      661 agaataaata aatccctaat aaaaattttg atacataccg catagacatc tcctccattt
      721 aaaaagtata ccatcgaaaa ccgctgaaaa atcataaaaa agtatgaagt ttaaggacca
      781 acgtagttat cgtacgaaaa ttagaagctt ttttttgttg ggaatccggt t
//
LOCUS       DAZUMK010000276          817 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000276 DAZUMK010000000
VERSION     DAZUMK010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.290"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            137..310
                     /locus_tag="ACG808_002986"
     CDS             137..310
                     /locus_tag="ACG808_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048519.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
BASE COUNT          274 a          131 c          103 g          309 t
ORIGIN      
        1 tttttcggag aattaaaaaa tacaataaat aactttagtt actttaatgc gttcttactt
       61 tatttttata ttatcatttt tcttattcac ttctttggtt tatactattc ttataaataa
      121 aaacgaggta tgtcctatga tgaaaagatt gtactattca ttgatcataa cgattggcta
      181 tttgattgta tcaaatctag gaaatatggt atttggtatc agtaaagaat tcagttggac
      241 tactacatta tgggaatctc tattcttttt catcttcgta tttttgttac aaaattatcg
      301 aaaaaaataa acatgtgcca tctatctgtt ggcacatgtt ttaggattta tggtactttt
      361 tattcggcaa tccattttat ttttctactt cttcttaata cctctaacac ttttgattta
      421 taaactaatc caatgcatct acttgcgctt attaagctat ttcagctaga tagtaccacc
      481 ccatgcgtca atgcttgatt ttccttttgt ataacattgt ataagcaata aaggacaaat
      541 aagtggtgat tacaccaaac cattgaataa gaaaaattct gatacgtaaa taaacatgaa
      601 cttgaaaaaa ttaatgaatc ggttagctac tacgtagttg tcttttttct catacagttc
      661 tgttaactta cacttctctt cgctagataa taaaaaatcc tccaactgta ttgtccaata
      721 taatcccagg catctaaact agcattatag aagagacaca catagaatct aaaataactg
      781 gttttaaatg tttcttacaa tcaaaaaaag ctgttaa
//
LOCUS       DAZUMK010000277          747 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000277 DAZUMK010000000
VERSION     DAZUMK010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.292"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(44..502)
                     /locus_tag="ACG808_002987"
     CDS             complement(44..502)
                     /locus_tag="ACG808_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5048520.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKLTRV"
BASE COUNT          220 a          128 c           97 g          302 t
ORIGIN      
        1 gacttccttt tttgtatgga ttttttcatt tacacaaaat attttatact ctcgtcaact
       61 ttctcttttc aaaaaaaatt tgatattttt ttagatttct ttttagtctt tgtaatactt
      121 tataagccac agaataactc acgtgatgct gtttagcaaa attttctact gagatatatt
      181 tttcctgaaa gaaagccaat accattttca tagataaaga atccgttata taacgagata
      241 acagagcttt gctagaacat tttccattaa tttccagaca tatatccaat tcactgacgg
      301 ttactcgcaa atttttctct agatcgaact tttgtaaatc atgtttcagc ttatcgatcg
      361 tttttttcaa gagaaatgtc gatagcgaca actcttcttg aacattcgat attgactctg
      421 ataaatttga gcttgcttct aaatatttaa gtaatgaata acttctgtga tcttctttgc
      481 ttaaaaaaaa ctcataattc attagtcatc agctctttct acgtatatta ctttccatgc
      541 attcaccaac tagctgggga taaaactgat ttcctggatc cgagtcactg ttgtcagcga
      601 ttcttgtttg tataatatac atcgtcctgt ctttttttta acattttgga gagcgtttta
      661 tttggccttt ctcgcgatca aacctgttat tagaattata tcatttacta gtatgcattt
      721 ttattaataa aatggtatat tttatat
//
LOCUS       DAZUMK010000278          746 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000278 DAZUMK010000000
VERSION     DAZUMK010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.293"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..668
                     /locus_tag="ACG808_002988"
     CDS             <1..668
                     /locus_tag="ACG808_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
                     /protein_id="HFS5048521.1"
                     /translation="NIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDI
                     ASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNA
                     TTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIG
                     TNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRT
                     YKD"
BASE COUNT          323 a           48 c          125 g          250 t
ORIGIN      
        1 ctaatattga atattcgtat attagtgatg aattatctat actaggttat aaagaaatta
       61 aaggaacttt tttaacacca gaaatttatt ctactatgtc agaagaagaa caaaatttgt
      121 taaaacgaga tattgccagt tttttaagac aaatgcacgg tttagattat acagatatta
      181 gtgaatgtac tattgataat aaacaaaatg tattagaaga gtatatattg ttgcgtgaaa
      241 ctatttataa tgatttaact gatatagaaa aagattatat agaaagtttt atggaaagac
      301 taaatgcaac aacagttttt gagggtaaaa agtgtttatg ccataatgat tttagttgta
      361 atcatctatt gttagatggc aataatagat taactggaat aattgatttt ggagattctg
      421 gaattataga tgaatattgt gattttatat acttacttga agatagtgaa gaagaaatag
      481 gaacaaattt tggagaagat atattaagaa tgtatggaaa tatagatatt gagaaagcaa
      541 aagaatatca agatatagtt gaagaatatt atcctattga aactattgtt tatggaatta
      601 aaaatattaa acaggaattt atcgaaaatg gtagaaaaga aatttataaa aggacttata
      661 aagattgatt atataatata tgaaaagcta ttataaaaga cattagtatt aaatagttta
      721 aaaaaatgaa aaataataaa ggaagt
//
LOCUS       DAZUMK010000279          731 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000279 DAZUMK010000000
VERSION     DAZUMK010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.295"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            40..731
                     /locus_tag="ACG808_002989"
                     /pseudo
     CDS             40..731
                     /locus_tag="ACG808_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612146.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          296 a          124 c          139 g          172 t
ORIGIN      
        1 gtgtgtagac tagaagaaaa cataccagga ggaattttta tgtctaagag aacacgaaga
       61 actttttcac aagaattcaa gcaacaaatc gtcaatcttt acttagctgg aaagccacgt
      121 gtagaaatca ttcgagaata tgaactaacg gcttcagcat ttgacaaatg ggtaaagcaa
      181 tctaaaacga gtggttcatt caaagaaaaa gataatctta cgcctgaaca aaaagaattg
      241 ttagaactac gtaaaagaaa ccagcaatta gaaatggaaa atgatatttt aaagcaagca
      301 gcgctgatat tcggacgaag agacaagtaa tcgatgcgaa taagcatctt taccctatat
      361 cagcgatgtg cagaatatta ggtctatcac gtcagtccta ttattatcaa tcaaaaccaa
      421 agaaagacga atcagaactt gaagaagtag tcgctgaaga atttatccgc agccgaaagg
      481 cctacggctc aagaaaaata aaaaaagcct tatcaaaacg aggcattcag atcagccgac
      541 gaaaaattag tagaatcatg aaaaatagag gattaaaatc gagctatact gttgcttatt
      601 ttaaagtaca tcattctact tgcaatgaag ccaaaacgac aaacgtattg aatcgtaaat
      661 tcttaagaga caacccatta gaagcgatcg taacagactt gacttatgta cgagtcggga
      721 aaaaatggaa t
//
LOCUS       DAZUMK010000280          730 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000280 DAZUMK010000000
VERSION     DAZUMK010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.296"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>730
                     /locus_tag="ACG808_002990"
     CDS             <1..>730
                     /locus_tag="ACG808_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFS5048522.1"
                     /translation="QINNMLNLNLKTYELNKIDLSLSHDFKINEVQYTQVSEMYHTML
                     EKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTRELQYNVIAKLLQN
                     NPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCYRSNQRLVDFYS
                     NFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIESGIHEKDICVLA
                     PQHYPLFSLGEYLKKELPYCNFDAI"
BASE COUNT          276 a           99 c          122 g          233 t
ORIGIN      
        1 caaattaata atatgctgaa tttaaatctc aaaacgtatg aactgaacaa aattgatttg
       61 tcactttctc atgattttaa aattaatgaa gttcagtata ctcaagtttc tgaaatgtat
      121 cacactatgt tggaaaaaaa taaagaaatt gattttgata tgattttact agtagcgtat
      181 aaaattttaa ctgtaaacag taacgttgca cgtaatattg ctagtaatat aagggcgatt
      241 tatgtggatg aatttcaaga tactagagaa ttacaataca atgtaatagc taaattactt
      301 caaaacaacc ctcaaattca gtcaatgttt gttggagata ttgaccaagc aatttatgga
      361 tcattagatg gtatagctaa atcagttgaa gaactagaaa tgctaactag tcacactttt
      421 caatcaaaga cgctacatgg ctgttacagg tctaatcaga gattagtaga cttctactct
      481 aattttcaga gttgtcctta cgagattgaa agcagaggga ataataaaaa cgaccgtggt
      541 tttataagtt atgattgcaa agttactaag gaggatttac ccaacataat aaaaaatata
      601 ataaaggaaa agattgaaag cggcattcat gaaaaagata tttgtgtact tgctcctcaa
      661 cactatcctc tgttttcact gggtgagtat ttaaagaaag aattaccgta ttgcaatttt
      721 gatgctataa
//
LOCUS       DAZUMK010000281          720 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000281 DAZUMK010000000
VERSION     DAZUMK010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.297"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..375
                     /locus_tag="ACG808_002991"
     CDS             <1..375
                     /locus_tag="ACG808_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048523.1"
                     /translation="DVESQQFGTNVINKVINPALSINAPLIPTALSFSVTAVVSEIED
                     TEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLVEEAGEHYAVVK
                     FNGTEIGRQIFDIKVNKPVEKN"
     gene            389..>720
                     /locus_tag="ACG808_002992"
     CDS             389..>720
                     /locus_tag="ACG808_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048524.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVL"
BASE COUNT          262 a           98 c          132 g          228 t
ORIGIN      
        1 gatgtagaaa gtcagcaatt tggcactaat gttattaata aagtaattaa tccagctctt
       61 tctattaatg cgcctttaat tcctactgct ttatcatttt ctgtaacagc tgtggtgtct
      121 gagatagagg atacagaaaa gataaaaata gtagaaattg aagttttaaa caagaatgaa
      181 aaacaaattt tttctacggg tgaagtatcg gtaagcttgc ctcctcaagt taacgatata
      241 aactttaata taaacgccag aaatgtcttg gttgaagaag caggagagca ttatgctgtt
      301 gttaaattta atggaactga gattggaagg caaatctttg atatcaaggt caacaaacca
      361 gtggaaaaaa attaaggaga cgatacggat gcttgatata gtaccatcaa atacacatcg
      421 gagtggtagc ttaataaatt ttgctgcgat ttttgcttgc gcatcggtaa ttctagcatc
      481 tccagttgtt gtggatgcaa gttctactcc gaaaactaaa aatgataatc aacttgtaat
      541 tactacaaaa aatgagatta acacaatttc aagtaatgat ggcaaaattt ttgatgtgat
      601 aaatacggtt ttaaaaagaa aagctgaaat caatataaaa tatgatgaag atttaaactt
      661 gtatttcttt gttatcaaaa caacatctga attgttttcc tcagattacg atgttttgga
//
LOCUS       DAZUMK010000282          696 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000282 DAZUMK010000000
VERSION     DAZUMK010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.298"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(53..628)
                     /locus_tag="ACG808_002993"
     CDS             complement(53..628)
                     /locus_tag="ACG808_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFS5048525.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          229 a          151 c          125 g          191 t
ORIGIN      
        1 atgaaaaaac agagccagat tagcattatt ttgctaatcc agctctgttt attcagccta
       61 tttttctttt tgaatatatc aagcgttggg gcaacatagt gatgactaat gaaacaacga
      121 cgatagacaa caagcgatca aggtagtctg tacccgcctg taccaagatc acacttgttg
      181 tctgacttaa acctaaccca tgtaatagct gaacgatcat actggatcct gaggaggtga
      241 tcccgccaaa taactgaacg gtaatgatcg aagaaacgat cgttccaggt accgacaaaa
      301 tcaaagcgag taataacagt ttcaagctgt ttttatcttt gccgagatat cttggcaaaa
      361 tcaatccagc aagcaagcct gtgagaattt ggattggcga ataatacaga gaaaaaatat
      421 ctgtagtcac tccacttaaa ataccgctgg acaatcctgt cagcattcca ggtacaggcc
      481 caagcaatgc accagcaaaa atcgttccga tagaatcgag atagattggc aaacgaagcc
      541 ataaagcaat cgttcctcca atataattga tcccaataca caaagccatc actgtaagta
      601 cgcgagttgc cattttgttt ttattcattt tgttagccca tctttaacct ttccaaatct
      661 ttcataatcg tttcttcttg tgcatttaaa agaacg
//
LOCUS       DAZUMK010000283          688 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000283 DAZUMK010000000
VERSION     DAZUMK010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.299"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..387
                     /locus_tag="ACG808_002994"
     CDS             <1..387
                     /locus_tag="ACG808_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFS5048526.1"
                     /translation="QSEQYLERNLAMLKLANHVGKGWFAIELGEKIDFLIKIPKYILN
                     AIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSASLKTSDTADLNIAIISLLEYF
                     GKDEDVVYLINQIENYTKILYAEVSK"
     gene            384..>688
                     /locus_tag="ACG808_002995"
     CDS             384..>688
                     /locus_tag="ACG808_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFS5048527.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     "
BASE COUNT          261 a           86 c          124 g          217 t
ORIGIN      
        1 caaagtgaac aatatttaga aagaaattta gcgatgttaa aactagccaa tcatgttggt
       61 aaggggtggt ttgcaattga gttaggtgaa aaaattgact ttttaatcaa aattcctaag
      121 tatattctta atgctattgc ttttgcatgt caggaaatag tagatctctc aatttataaa
      181 aaaattctac tccatacaat cgacttgtac gaggaaacta cagaattgtc agctttatca
      241 gctagtttaa aaacaagtga tacagctgat ttgaatatag caataatttc tttattagag
      301 tactttggta aagatgagga tgttgtgtat ctaatcaatc agatagaaaa ttacacaaaa
      361 atactttatg ctgaggtttc aaaatgacaa atatcttcga aaaaatcgta aatgatttaa
      421 atttagaaca aaaagatgtt gtggaaagtg aagataacct ttatgtaaat gcatgtcctg
      481 ggagtgggaa aacaagggta cttacaagga aaattgctta ccaagcaatt aaacacgatg
      541 gatcactaaa aagaataatt gctatcacat acactaatag agctgctgaa gaaattaagg
      601 aacgcctaag tttacttagt atagatgatg atattaatat ttgggtgggg acaatacatc
      661 agttttgtct tgaatttatt atttatcc
//
LOCUS       DAZUMK010000284          670 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000284 DAZUMK010000000
VERSION     DAZUMK010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.301"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(19..216)
                     /locus_tag="ACG808_002996"
     CDS             complement(19..216)
                     /locus_tag="ACG808_002996"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFS5048528.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(362..>670)
                     /locus_tag="ACG808_002997"
     CDS             complement(362..>670)
                     /locus_tag="ACG808_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS5048529.1"
                     /translation="LGVKGQNLLADDSFWQIEAFYLAQCAYNTTLMFSPDRIIFGGGV
                     MKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGIIGGLALARKAVRNKQP
                     "
BASE COUNT          230 a          134 c           95 g          211 t
ORIGIN      
        1 tacggctgaa aaaatctatc accaaaaaaa ttcaaacatt aagccaagga ttatgaagtc
       61 aacatttcga aaaatgaaca tacattggta agcaactata actattacca cagacataaa
      121 gtaacacaat atattactat ttaagtgtac gccaccagtg aatatcctca aggtatacag
      181 cgcacaacaa aaagaaaaaa ggaaacgcag aagcatttcc tttcagtgat ttaatgaaaa
      241 caacacgatt tgacttagag cctttttatt tgaattcatt ttcgctttat ccaacttcat
      301 ttgatcattt gattactcgg ttaaatactt ttgacatgag tatcaatgaa acattcatta
      361 attatggttg cttatttctt accgcttttc tggccaatgc taatccgccg attattccgg
      421 cattatcacc taattcgggc gtaataatgt agctatcaat tggtggtatt tcaacatatc
      481 catttataag ctcaacaaat ttatcctgaa cttttttctt catgtgttct tgtttcatta
      541 caccaccgcc aaaaataatt ctatctggcg aaaacatcag tgttgtattg tatgcacatt
      601 gggcaaggta aaaggcttca atctgccaaa acgaatcgtc agccaataaa ttctggcctt
      661 ttacacccaa
//
LOCUS       DAZUMK010000285          662 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000285 DAZUMK010000000
VERSION     DAZUMK010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.302"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..624
                     /locus_tag="ACG808_002998"
     CDS             85..624
                     /locus_tag="ACG808_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048530.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
BASE COUNT          242 a           86 c          123 g          211 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 ca
//
LOCUS       DAZUMK010000286          662 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000286 DAZUMK010000000
VERSION     DAZUMK010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.303"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..639
                     /locus_tag="ACG808_002999"
     CDS             <1..639
                     /locus_tag="ACG808_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFS5048531.1"
                     /translation="IAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRGVQEVLL
                     FITDGLKGMKDTIHQIYPKAKYQHCCIHVSRNIAHKVRVKDRKEICDDFKAVYQANSK
                     EEANTFLSGMIEKWKKNYPKVTQSLIENQDLLTFYDFPPSIRRTIYSTNLIESFNKQI
                     KRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          243 a          121 c          114 g          184 t
ORIGIN      
        1 attgccattg gtatacgaga agacggcact aaagaagtac tgagttatgc gattgctcca
       61 actgaatcaa catacgtttg gaatgagctg ctacaggata ttaactccag aggagttcaa
      121 gaagtcttgc tttttattac ggacggctta aaaggcatga aagatactat ccatcaaatt
      181 tatcctaaag caaaatatca gcattgttgt atccatgtat ctcgtaacat cgctcataaa
      241 gtacgtgtca aagaccgaaa agaaatctgt gatgacttta aggctgttta tcaagctaac
      301 tcaaaagaag aagcgaatac cttcttatcc ggcatgattg agaaatggaa gaaaaactat
      361 cctaaagtga cgcagtcact catagaaaac caagacttat taacttttta tgattttcca
      421 cctagcattc gtagaaccat ttactcaacc aatctaatcg agtctttcaa taagcaaatt
      481 aaaagataca gccgtagaaa agagcagttt caaaatgaag aatcactaga acgctttcta
      541 gtcagcattt ttgatacata caatcaaaaa tttctaaaca gaagccataa aggttttcaa
      601 caggtaaccg atacattagt ttcaatgttt actgagtaac taattatttt gcaggaggac
      661 aa
//
LOCUS       DAZUMK010000287          641 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000287 DAZUMK010000000
VERSION     DAZUMK010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.305"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          216 a          111 c           98 g          216 t
ORIGIN      
        1 aaaacctatt tcgcaaaaat tatttgtaag cattaaggat tttctgctga ctttcactgg
       61 aaggataacc caagaattct gaccaaagtg aaagattaag aaccatcttt gccagtctat
      121 ctatgtgcta ttgtaactgt caacatcatg cgggcagtta ttattattta tttgaataat
      181 atttttatta taaatcagtt ttttagattg tgcataatca ctaattgctg tgattaatta
      241 cagaatatgg ctctctggga aaatgtgcgc gtcatgtatt caattttaaa ttctaatttg
      301 tacaaaatga atcgtcacga ctcgatctga cgcaactcca aatgggggcg ccgacctgta
      361 ccaagtacag tggcatgttc tttaaacatt tgaagcaaaa tctgtccatc aaaaacctgt
      421 attttcaaag tgaacaaggt gcgctaaatc aggaaatact tacactgatc atttcttaat
      481 ttacctactt aaccaaaatt taaatcaata tgaaagcaac acttttcaaa ctaaagaggt
      541 ctttttaccg cttgatgttt gcttcgataa aaaagttgat gtcggattag ttttcatttt
      601 aataaaacgt actattcctg aaaaaaattt atccactact a
//
LOCUS       DAZUMK010000288          636 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000288 DAZUMK010000000
VERSION     DAZUMK010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.306"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>636)
                     /locus_tag="ACG808_003000"
     rRNA            complement(<1..>636)
                     /locus_tag="ACG808_003000"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          128 a          209 c          134 g          165 t
ORIGIN      
        1 ccaacactta gcactcatcg tttacggcgt ggactaccag ggtatctaat cctgtttgct
       61 ccccacgctt tcgagcctca gcgtcagtta cagaccagag agccgccttc gccactggtg
      121 ttcctccata tatctacgca tttcaccgct acacatggaa ttccactctc ctcttctgca
      181 ctcaagtctc ccagtttcca atgaccctcc ccggttgagc cgggggcttt cacatcagac
      241 ttaagaaacc gcctgcgctc gctttacgcc caataaatcc ggacaacgct tgccacctac
      301 gtattaccgc ggctgctggc acgtagttag ccgtggcttt ctggttagat accgtcaagg
      361 gatgaacagt tactctcatc cttgttcttc tctaacaaca gagttttacg atccgaaaac
      421 cttcttcact cacgcggcgt tgctcggtca gactttcgtc cattgccgaa gattccctac
      481 tgctgcctcc cgtaggagtt tgggccgtgt ctcagtccca atgtggccga tcaccctctc
      541 aggtcggcta tgcatcgtgg ccttggtgag ccgttacctc accaactagc taatgcaccg
      601 cgggtccatc catcagcgac acccgaaagc gccttt
//
LOCUS       DAZUMK010000289          621 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000289 DAZUMK010000000
VERSION     DAZUMK010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.307"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>621
                     /locus_tag="ACG808_003001"
     CDS             <1..>621
                     /locus_tag="ACG808_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS5048532.1"
                     /translation="LGAGIYGLAVTGINIPSKLADGGVTGIALLLHNLFGFAPSVTSL
                     LFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFAVDSLLINSLMTGIL
                     SGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGILVVSLLYLDLTHFL
                     YTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLEQL"
BASE COUNT          151 a          113 c          117 g          240 t
ORIGIN      
        1 ttttaggcgc aggaatctac ggattagctg tgacaggaat caatattcca tctaagctgg
       61 cagatggtgg ggttactggt attgctttgc ttttgcataa tctttttgga ttcgctcctt
      121 ctgtcacttc actgcttttt aatttacctt tattgcttat ctcattgttt ctttttggaa
      181 aacacgcttt tctccggacg atcgtcggaa catttgcgct agttttcttt ttacatttat
      241 gggagagcct tcatatacat tttgctgtag atagtctact gatcaatagt ttgatgacag
      301 gtattctttc tggcattggt tgcggtctcg tctttcgatt cggcggaagc acaggcggaa
      361 cagatatcat ttatcaagcc attgaaaaat attttcatat taagattggc aaaagtcttt
      421 tcttgattac ttttggtatt ttagttgtct ccttgcttta tttggatctc acacattttc
      481 tgtatacatt acttagctgt tctattctat cttatacatt gaataaagtg aagtatctgg
      541 aaattggatc tgttttgccc caattggaag caaaacagac tacagaaaca tttgaaccac
      601 ttgatcctct tgaacagtta g
//
LOCUS       DAZUMK010000290          598 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000290 DAZUMK010000000
VERSION     DAZUMK010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.310"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            141..506
                     /locus_tag="ACG808_003002"
     CDS             141..506
                     /locus_tag="ACG808_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048533.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
BASE COUNT          178 a          104 c           85 g          231 t
ORIGIN      
        1 gaaaaacttt ttttttagag ataatggcat gagagtttat tcatttaatc tagaaataat
       61 gaaaaagtaa ttgattattg caggattcaa tttacaataa aactaacaaa ataataacac
      121 ttgttaggtg gtgatgaaag atgataaaaa cttgctggaa aaaccttccg ttgcttttat
      181 cttttgttcc ttatgtgcat ttcgccttac tgcttgattt tcgttatcat tccgtaagcg
      241 ggttcatcac attgatattt ttatctcttt ttgctggata ttattttcaa aggaacaggc
      301 gtattatatc tttattcatt gccaatatta tatctactgt cacttcctat ttattttgcg
      361 ccaattttac tgaatggcgt tacttctatc atcctttaaa accgacccag ctgatcttgc
      421 ttcttgcagg tatttactta gttccacaaa tccttgggtc gttatgggcg gtagccttat
      481 cttataaaaa agctcggcat ccataaaaac aaaccatata tgtacaaaga gaagctactt
      541 tcttcttttt atacatatat gtttttctta tatcaccaaa actatcacta gtttttct
//
LOCUS       DAZUMK010000291          588 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000291 DAZUMK010000000
VERSION     DAZUMK010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.312"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(65..150)
                     /locus_tag="ACG808_003003"
     tRNA            complement(65..150)
                     /locus_tag="ACG808_003003"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Leu,seq:taa)
     gene            complement(167..238)
                     /locus_tag="ACG808_003004"
     tRNA            complement(167..238)
                     /locus_tag="ACG808_003004"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(204..206),aa:Gly,seq:gcc)
     gene            complement(250..322)
                     /locus_tag="ACG808_003005"
     tRNA            complement(250..322)
                     /locus_tag="ACG808_003005"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(287..289),aa:Thr,seq:tgt)
     gene            complement(340..421)
                     /locus_tag="ACG808_003006"
     tRNA            complement(340..421)
                     /locus_tag="ACG808_003006"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(385..387),aa:Leu,seq:tag)
     gene            complement(429..501)
                     /locus_tag="ACG808_003007"
     tRNA            complement(429..501)
                     /locus_tag="ACG808_003007"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(466..468),aa:Lys,seq:ttt)
     gene            complement(505..577)
                     /locus_tag="ACG808_003008"
     tRNA            complement(505..577)
                     /locus_tag="ACG808_003008"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(542..544),aa:Val,seq:tac)
BASE COUNT          149 a          169 c          138 g          132 t
ORIGIN      
        1 cccctaacct tttgatccgt agtcaaacac tctatccagt tgagctatgg gcgcatattt
       61 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
      121 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      181 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      241 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      301 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      361 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      421 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      481 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      541 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DAZUMK010000292          585 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000292 DAZUMK010000000
VERSION     DAZUMK010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.313"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..>585
                     /locus_tag="ACG808_003009"
     CDS             51..>585
                     /locus_tag="ACG808_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFS5048534.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNK"
BASE COUNT          216 a           92 c          116 g          161 t
ORIGIN      
        1 tactatcaaa taaggtagaa taatagaaaa agatagcagg aggaatgacg atgaatcatt
       61 ttaaaggaaa gcaatttcag caggatgtga ttattgtagc cgtgggctac tatcttcgtt
      121 ataaccttag ctatcgtgaa gttcaagaaa tcttatatga tcgtggcatt aacgtttctc
      181 atacgacgat ttatcgttgg gtgcaagaat atggcaaact actctatcaa atttggaaaa
      241 agaaaaataa aaaatccttt tattcatgga aaatggatga aacgtacatc aaaattaaag
      301 gaaaatggca ttatttgtat cgagccatcg atgcagatgg tttaaccttg gatatttggt
      361 tacgtaaaaa acgggacaca caagcagcct atgcttttct taagcggtta gtgaagcagt
      421 ttgatgaacc gaaggttgta gtcacagata aagccccctc tattacaagt gcctttaaga
      481 aactaaaaga atacggcttt tatcaaggga cagaacatcg taccattaaa tacctgaata
      541 atttgattga acaagaccat cgtccagtaa agagacgcaa taaat
//
LOCUS       DAZUMK010000293          583 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000293 DAZUMK010000000
VERSION     DAZUMK010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.314"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..286)
                     /gene="tnpB"
                     /locus_tag="ACG808_003010"
     CDS             complement(<1..286)
                     /gene="tnpB"
                     /locus_tag="ACG808_003010"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFS5048535.1"
                     /translation="MRKGIDGLASLIQQEYQLELYEDAVFLFCGNRQDRFKLLYWDGD
                     GFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLSIDQPRAILPGKK"
     gene            complement(333..527)
                     /locus_tag="ACG808_003011"
     CDS             complement(333..527)
                     /locus_tag="ACG808_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048536.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          168 a          123 c          100 g          192 t
ORIGIN      
        1 ctttttttcc tggcaaaata gctcgaggtt gatcgatact taatccttct aacaaccatc
       61 gaacctgttg gttcgttaag gttcgaactt cgtctttcgt ccgaggccat tttaacttgc
      121 cgttttctat ccgcttataa cagagaagaa aaccatcacc gtcccaatag agaagtttga
      181 aacggtcttg acggttcccg caaaataaaa aaacggcatc ttcgtaaagc tcgagttgat
      241 actcttgttg aataagactg gcaagaccat caattccttt tcgcaaatct gtttttccgc
      301 acacaatata aatatgtttg gtttgaagta aatcataaag cattggacaa cagttctttc
      361 aaaatagctt gaagaatata attgttaata ccgttataga tcaaaacttc agaaccattc
      421 tctcttttga tgcgcatcgc taacttggca gccgcgggtt tacctttttc tggtgcactc
      481 ttttcgtttc ctaattggac aggaacaatc tcatttaatt tatacataga aaacctccag
      541 acttcttgat agctcaagta tatcttgagg tgcatttgtt agt
//
LOCUS       DAZUMK010000294          577 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000294 DAZUMK010000000
VERSION     DAZUMK010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.315"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>577
                     /locus_tag="ACG808_003012"
     CDS             <1..>577
                     /locus_tag="ACG808_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS5048537.1"
                     /translation="VVQGWTPDVIVGRAAFPISCSARTIYRMFKKGLFDSSDLPMKGK
                     RKPNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHLEGDTIVGLKHKSAVITLVERLS
                     KVIITLKPCGRQAIDIEKKLNQWFESVPKNLFKSITFDCGKEFSNWKQISNVNDIAIY
                     FADPGTPSQRGLNENSNGLLRRDGLLKSMDFN"
BASE COUNT          186 a           98 c          119 g          174 t
ORIGIN      
        1 gttgttcaag gatggacacc cgatgtgatt gttggtcgtg cagcgtttcc tattagttgt
       61 tctgctcgta ccatttatcg tatgttcaaa aaggggctat ttgattcttc tgacttaccg
      121 atgaaaggca agcgtaaacc gaatggacat caagaaagac gtggaaaaca aactttccgc
      181 cgctctattc atgaacgtga aaaggattat agccaattct caaatgagtt tggtcacctt
      241 gaaggtgaca ctatcgtagg tctgaaacat aaaagtgctg taattacctt agttgaacga
      301 ttatcaaaag ttatcatcac attgaaaccg tgtggtagac aagcgattga tattgaaaaa
      361 aaattaaatc aatggtttga atctgtaccg aaaaacctat tcaaatccat cacttttgat
      421 tgtggaaagg aattttcaaa ttggaaacag atcagtaatg tcaatgatat tgccatttat
      481 ttcgctgatc caggaacgcc gtctcaaaga ggcctaaacg agaattctaa cggattgtta
      541 cgtagagatg gtttattgaa atctatggat ttcaatt
//
LOCUS       DAZUMK010000295          576 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000295 DAZUMK010000000
VERSION     DAZUMK010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.316"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>576
                     /locus_tag="ACG808_003013"
     rRNA            <1..>576
                     /locus_tag="ACG808_003013"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          184 a          114 c          166 g          112 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatcta
//
LOCUS       DAZUMK010000296          565 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000296 DAZUMK010000000
VERSION     DAZUMK010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.317"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            425..>565
                     /locus_tag="ACG808_003014"
     CDS             425..>565
                     /locus_tag="ACG808_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS5048538.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYL"
BASE COUNT          207 a           89 c           84 g          185 t
ORIGIN      
        1 cgtttctaaa tattccaatt tgggaatcca ataaaaattt tcgttcaaat acgtgtctat
       61 tcgaactctt ttctagttgt taattatgaa gggggaagaa ataaaaaata caatctatac
      121 acgaataaga atattatcca aaaggaggtt ttagaaaaga tttattatac gtaaaaagct
      181 caagacattt tcttagccat aaggacatgt ttgatattta agaagatata tgaattttct
      241 tttctaagta taaagataac tatccattat caactaaaaa aacatttttg aaaataaaca
      301 tcagcagtcc tatcaatgca tgtgagctta tagaagttat tcgcagcgca gcataattgc
      361 attagcttct tttcgtgttt tttgcctctt ttccctttaa atcttaatgg aggaggtgtg
      421 caatatgtca gataacgacg aacgcgtaaa tgcccagttg attcgattct ttgaaaaggt
      481 cattttgaac accgctaata cttattataa aaaacaagaa gaaattcaaa aacatgaaca
      541 acttgatcaa ctcccaccat atttg
//
LOCUS       DAZUMK010000297          549 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.319, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000297 DAZUMK010000000
VERSION     DAZUMK010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.319"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          197 a          101 c           79 g          172 t
ORIGIN      
        1 aaaaatttca aaaatgacct gttaaatggt catcgaatat catttaaaat caatacgacg
       61 gagtcagaca cggagtcaaa acaagcccgc tgagagtaaa aaatatacag tcccataggt
      121 aaagtgccac aggactgtat atttttttat ttgagattac ctgaaatgat tccatttaat
      181 ttttatctca ttttttccta ctagttagta tcaaatactc tcctattttc tcaataaact
      241 attttgggtt acatggtatc tgaaacagct aaaaccttgt cattatagca aaaaatcact
      301 ttatctccgt ttcatttcag atacatttca cttaagcgga ttccaaaaaa acaaaagaaa
      361 aaggaacccc tagttagcct atgcttttta ttggttcctc tcaaaacacg gtcatataac
      421 aacggggtgg ggtaaatgaa tgaaaaaggt ggaggaaaca ttattcaata gctgtcttaa
      481 atacttcgtt ataaaatgtg aacaaataaa agacatctat ccctattttc tacacttcca
      541 cttgactac
//
LOCUS       DAZUMK010000298          546 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.320, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000298 DAZUMK010000000
VERSION     DAZUMK010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.320"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>546
                     /locus_tag="ACG808_003015"
     CDS             <1..>546
                     /locus_tag="ACG808_003015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS5048539.1"
                     /translation="SLISNLDKKIISFTARYDINIKGFDYLCEVAKNLPVGWAIRIAG
                     DGTEEERKELKRLIEKNGVLDKIICAGALFGDELINHYIESSIFISTSRSEGLPLVLI
                     EAMTYGLPVISFDHGGAKEILQDGNYGILIKNFDVQDMNNEIEKLSNNRLLMEKYQKF
                     SLERAKDFNEKKIIGEWQTLL"
BASE COUNT          213 a           48 c          107 g          178 t
ORIGIN      
        1 ctagtttaat aagtaacctt gataaaaaaa ttatttcatt tactgccaga tacgatataa
       61 atattaaagg gtttgattat ttatgtgaag tagctaaaaa cttacctgtt ggatgggcta
      121 taaggattgc tggagatggt acagaagaag aacgtaaaga gctgaaaaga ttaattgaga
      181 aaaatggtgt gttagataaa attatttgcg caggtgcatt atttggagat gaattaatca
      241 atcattatat agaaagttct attttcatat ctacttctag aagtgaggga ctaccattag
      301 tgttgataga agcaatgact tatggattac ctgtaataag ttttgatcat ggtggtgcta
      361 aggaaatatt acaagatgga aattatggaa ttttaattaa gaattttgac gtacaagata
      421 tgaataatga gatagaaaaa ttgtctaata atcgtttact tatggaaaaa tatcaaaaat
      481 tctctttaga aagagctaag gattttaatg aaaaaaaaat aattggtgaa tggcaaacat
      541 tgttaa
//
LOCUS       DAZUMK010000299          545 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.321, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000299 DAZUMK010000000
VERSION     DAZUMK010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.321"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(165..464)
                     /locus_tag="ACG808_003016"
     CDS             complement(165..464)
                     /locus_tag="ACG808_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFS5048540.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          174 a          101 c           65 g          205 t
ORIGIN      
        1 atactagctg tttttgtgtt aattaaagga taaaatacaa tataaagaaa catctgtttc
       61 tttatttaga ataaaaaaat acagactctg ttcaaataaa tgaacctagc ctgcattatg
      121 agattgacta tactttttaa ttcattgaga tgaataactc gtttttataa atgcccaaaa
      181 gtaatcggtg ccgaagccat tcctcgacct aattcttttt caaattttcc tattgttgat
      241 atctccatat aaaacggtga tactggttta gacaattctc ccctgattgc taacggacgt
      301 aaactacgtt ccaaattatt tatgacttta ggataatact gacctttttc taagcctgtc
      361 tttgcgtcta tcaaaagtat acgttccttt tctttgatct ctggatttaa aacgagatca
      421 tataatttta gtaccatgtt ttctatttct tcacttttag acatcacgat ttcctcccaa
      481 aaatatattt cttcccacta cattctataa acaaagtata cgctacatct agattttttt
      541 aaatc
//
LOCUS       DAZUMK010000300          539 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000300 DAZUMK010000000
VERSION     DAZUMK010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.322"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..506
                     /locus_tag="ACG808_003017"
     CDS             <1..506
                     /locus_tag="ACG808_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
                     /protein_id="HFS5048541.1"
                     /translation="YLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDIW
                     LRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVKY
                     LNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEIK
                     VLMGITA"
BASE COUNT          183 a           99 c          114 g          143 t
ORIGIN      
        1 attatctttg gaagaagaaa aatagacaat ccttctattc atggaaaatg gacgaaacct
       61 atatcaaaat taagggacgt tggcattatc tttatcgtgc aattgatgcg gacggcttaa
      121 ccttagatat ctggttacga aagaaacggg atacgcaagc agcctatgct ttcttaaaac
      181 gactccataa acagtttggt gagccgaaag caattgtgac cgataaagca ccttctcttg
      241 gctccgcctt tagaaagtta cagagtgtgg gtttatatac taagacagag caccgaactg
      301 tgaagtatct taacaattta atagaacaag accatcgacc tattaaacga cgaaataaat
      361 tttatcaaag tctccgtaca gcctcttcca cgattaaggg catggagacc attcgaggaa
      421 tatataaaaa gaaccgaaga aatggaacgc tcttcggctt ttcggtgtct actgaaatca
      481 aggtattaat gggaataaca gcctaagata tttggagttc agagagggcg cgtttgatt
//
LOCUS       DAZUMK010000301          531 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.323, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000301 DAZUMK010000000
VERSION     DAZUMK010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.323"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            136..>531
                     /locus_tag="ACG808_003018"
     CDS             136..>531
                     /locus_tag="ACG808_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS5048542.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSF"
BASE COUNT          178 a           91 c          100 g          162 t
ORIGIN      
        1 gctctataat ttatgggact gatgaatcag aattgattct tcagccccat ttttatttta
       61 ggtattaaaa aacatatcgt tctctagtat gattaaatca cgacaaaata aacaactgag
      121 aggacgatat gctatatgaa tgattctatc aaaaaaatgc tgcgattaat agagaaagat
      181 ttgatgatta cagaggtctc ttacgagacc tttcagaaga aaaagacgtt gatcgtcgat
      241 gctgttttct cacctgctcc ccatacctgt agaaattgtg gttctactgt ggtagatgga
      301 aacgggaaag taattgtagt gaaaaatggg aagaaggaaa cgattgtccg ctttgaacaa
      361 tacaatcaca tgcctttggt tatgcgctta aaaaagcagc gctatacctg taaaaactgt
      421 cgaacccatt ggacggctca aagttatttt gttcaaccca gacattcaat cgcgaatcat
      481 gtcagatata aaattgcttc tttactgact gaaaaagtat ctttatcttt t
//
LOCUS       DAZUMK010000302          528 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.324, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000302 DAZUMK010000000
VERSION     DAZUMK010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.324"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            52..>528
                     /locus_tag="ACG808_003019"
     CDS             52..>528
                     /locus_tag="ACG808_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5048543.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSV"
BASE COUNT          199 a           89 c           85 g          155 t
ORIGIN      
        1 attctgtaaa attaaagtta ccacacagaa attttatagg aggactttct catgactaaa
       61 tttactatag aaattatgca aacactactc aataaagggg atttagatga attatttcag
      121 gatcatttag agcgagcgat taattcactt ctacaagcag agctaactgc ttttttagat
      181 tacgaaaaat atgatcgaaa tggattcaat tcaggaaatt cccgtaacgg gaattattca
      241 cgtacattta aaacagaata tggcgagttg aatttaacta ttcctagaga tcggaatggc
      301 gaattttctc aacaaacact accagcatat aagcgaacca atgattcatt agaaacgacc
      361 attatccaat tatttcaaaa aggcattact atggctgaaa tctccaaatt aattgaaaaa
      421 atgtatggtc accactatac gccacaaaca atctccaaca tgagtaaatt ggtggctgaa
      481 gatgttttag cttttaaaga aagaacgtta gaagccaatt attccgtt
//
LOCUS       DAZUMK010000303          517 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.325, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000303 DAZUMK010000000
VERSION     DAZUMK010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.325"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(113..238)
                     /locus_tag="ACG808_003020"
     CDS             complement(113..238)
                     /locus_tag="ACG808_003020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048544.1"
                     /translation="MHLHRISALAVQIGRYHHSETRAVMVTRCCLLALVIAAANA"
BASE COUNT          169 a           87 c          117 g          144 t
ORIGIN      
        1 tatccataaa actaatataa agcttggtac gattctgttt gtaagacata cgaatagtat
       61 cagaggtagg agccaagctg cctgatatta aattatcgat aaagtagaaa ggttaagcat
      121 tagccgctgc aataactaat gctaggagac agcaacgtgt aaccattacc gcacgtgttt
      181 ctgaatggtg gtagcgaccg atttgaacgg ctagggcgga aatacggtgc aaatgcacgg
      241 tggtttcatg caatgcttac agggcgtatt ttgtaagggt atgtaacgga gataattccg
      301 acggtaatgg atgtgagagg ccaagccgac gctgacggta gggcaaacaa acatagttgc
      361 actctagcga tcgttattta tttttaacgg tcaaactagg gtacaactat gcttaaaaca
      421 cttcgcctca acttctggta gcaaaagtga ttttgaaagt caaaatattt tttatgagtt
      481 atttacaatt ctaaaaaaca agtgtattaa tctacta
//
LOCUS       DAZUMK010000304          514 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.326, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000304 DAZUMK010000000
VERSION     DAZUMK010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.326"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACG808_003021"
     CDS             complement(<1..132)
                     /locus_tag="ACG808_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFS5048545.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DAZUMK010000305          498 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.328, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000305 DAZUMK010000000
VERSION     DAZUMK010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.328"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            258..498
                     /locus_tag="ACG808_003022"
                     /pseudo
     CDS             258..498
                     /locus_tag="ACG808_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611995.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          169 a           91 c           69 g          169 t
ORIGIN      
        1 ttttacactc tctattattc ttttcataaa tattgtaaat agaagtatta aaaatatata
       61 caaaaagtat tatttcgaga aagataaata ttttcaaaaa ccttttcata atcctcaccc
      121 tttcttacac tgataaatca tgcgtctaat attcaaatta tactcgatat gctttagcta
      181 atcagccaac tctgtttgtc tactatcatt atatcaatag acaatatctg ctcccgtctt
      241 tatgatcgtg ccaaagcaat caaaagaaaa ttgcgaatca cttagatcga aagtttactc
      301 aaacaaaagc attgtcagcg gttgtcacgg atttaactta tgttcgggtc gggacaaatt
      361 gggcttatat ttgtttgatg attgatttat tcaatcgtga aattattggc cattcttgcg
      421 gaaataaaaa agatgcgcag cttgtaaaaa gggccattca aagcattcct tactcacttc
      481 aagaaatcga actcttcc
//
LOCUS       DAZUMK010000306          495 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.329, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000306 DAZUMK010000000
VERSION     DAZUMK010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.329"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(36..>495)
                     /locus_tag="ACG808_003023"
     CDS             complement(36..>495)
                     /locus_tag="ACG808_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048546.1"
                     /translation="FYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLIS
                     EPVKIIIDFSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESAS
                     QVVVWLDPPRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          155 a           89 c           79 g          172 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaat
//
LOCUS       DAZUMK010000307          479 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.332, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000307 DAZUMK010000000
VERSION     DAZUMK010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.332"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            73..234
                     /locus_tag="ACG808_003024"
     CDS             73..234
                     /locus_tag="ACG808_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFS5048547.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            253..>479
                     /locus_tag="ACG808_003025"
     CDS             253..>479
                     /locus_tag="ACG808_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5048548.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEK"
BASE COUNT          187 a           48 c           82 g          162 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatt
//
LOCUS       DAZUMK010000308          462 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.334, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000308 DAZUMK010000000
VERSION     DAZUMK010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.334"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          159 a           63 c           64 g          176 t
ORIGIN      
        1 ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg tacattatgc
       61 tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac ttaatccata
      121 gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca ccccaaacca
      181 cttccccata agtgtattac gctttaaact cttttatatt tgaattcact aagaagcata
      241 ccatatattt gaatttacta agaagcatac catatttttg aaaaaaagtg agaaaaaagg
      301 cttataatta ggatttagag taattaatta gatgtttatt ttataggact gatactatag
      361 attacaaata gtatcgtatt tcgcaatctt aaaattatct tcttactgtg aataagataa
      421 taaaatttgt atttgtcctt tttaggtaac taattttaat ct
//
LOCUS       DAZUMK010000309          447 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.336, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000309 DAZUMK010000000
VERSION     DAZUMK010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.336"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          124 a           97 c           78 g          148 t
ORIGIN      
        1 ttttcaatag ctataaatta tttaatagat aagttaaggg gtgcataaac tgcatccctt
       61 aacttatctt attttgctta aacaatcttg gcccgtaaac tgcataactc atatgtaaat
      121 cgctcctttt taggtggcac aaatgtgagg cattttcact atttccggca attacttcca
      181 tgtaaagtat aacccactat actttgcaag caaagtgtgg gctcgccgag gggtcttgca
      241 gacggctctt tcggctcttt tgagccgagt atcagcgcac ccttatggtg caattagctg
      301 atacctgttt gacgatttgc tgccctacct tttcggtagg tatgcaaacc tcaaaccaaa
      361 ataaaaaccc ccttcgactt cgtcaggggg tttttattta tataaatctc cgtaaataat
      421 cgcgatttaa taacatcttc aatttta
//
LOCUS       DAZUMK010000310          442 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.338, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000310 DAZUMK010000000
VERSION     DAZUMK010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.338"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(201..323)
                     /locus_tag="ACG808_003026"
     CDS             complement(201..323)
                     /locus_tag="ACG808_003026"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048549.1"
                     /translation="MNMNRDVLKMNEFDQSLSYRNLQFRSIGLSINEKVKKNMF"
BASE COUNT          135 a           87 c           52 g          168 t
ORIGIN      
        1 attacgttat taaaaaaaca gatgaaagat caggttttat tagatctcat tctttcatca
       61 cttgaaggga tttaatgaac tactttttcc tctttgtcgt ttttatatat tcgtattcct
      121 tcaaacagta ggatagcctc tttcaatgct tgatatttga actgtccgtt acttaaaaat
      181 ttagatgtgc cgccctcttc ctagaacata ttctttttta ctttttcatt aattgacaga
      241 ccaatcgaac gaaactgtaa atttcgatag gataatgatt ggtcaaactc attcattttc
      301 aagacatctc gattcatatt caagatagga atctttttat caacatcact ttattcgtat
      361 tttcctctac cataaaagct tcacgctcca caaaattgtt ttatacacta ctattcagcc
      421 cattcaacga ccaatgaatt tt
//
LOCUS       DAZUMK010000311          437 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.339, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000311 DAZUMK010000000
VERSION     DAZUMK010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.339"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          128 a           76 c           58 g          175 t
ORIGIN      
        1 agaaattgtg cattatttct tcacatttat taggttatag tttaagaaag tttaaacatt
       61 gcgcaattta attattttag ctgtttcttt tcataaaaaa acatgttgtg actctgagac
      121 aaatcatagt ccatagtgat gtctctagag taactacgcc taatattacg aggcatcata
      181 ttaatctatc agcactagta cctaaactag catccctttc gaatattttt tcttatataa
      241 taaagtttgc tttcactcct ctaatctcac caaatcgact ggcttgcgtt gtgtgatact
      301 aggaactgat tggtttagaa cgacttttct attgtaatca tatttttcgc catcctcgtt
      361 gtcttctaat ccttcaatga atgatcccta atgtgtgtat taaataattt aatttttttg
      421 ttcctattta tttaaca
//
LOCUS       DAZUMK010000312          408 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.341, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000312 DAZUMK010000000
VERSION     DAZUMK010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.341"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>408
                     /locus_tag="ACG808_003027"
     CDS             <1..>408
                     /locus_tag="ACG808_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5048550.1"
                     /translation="NTLLRTELTAFLDYEKYDRTGFNSGNSRNGSYFRSIKTEYGELT
                     LEIPRDRNGEFKQQTLPAYKRTNDTLETTIIHLFEKGVTMSEIADLIEKMYGHHYTPQ
                     TMSNMTKVLTEEVNAFKSRALNDKYVAIFMDAT"
BASE COUNT          148 a           75 c           67 g          118 t
ORIGIN      
        1 aaacacactc ctacggactg aattaacggc ttttttagat tacgaaaaat atgatcgcac
       61 tggttttaat tcaggtaatt cgagaaacgg ttcttacttt cgatcaatca aaaccgaata
      121 tggtgaatta acattggaaa tacctagaga tcgtaatggt gagtttaaac aacaaacttt
      181 accagcctac aaaagaacaa acgatacatt ggaaaccact attatccatt tattcgaaaa
      241 aggtgttacg atgtctgaaa ttgctgattt gatcgaaaaa atgtacggtc atcactatac
      301 tccacaaacc atgtccaaca tgactaaagt tctgactgaa gaagtaaatg cctttaaatc
      361 cagagcctta aatgataagt atgtcgctat ttttatggac gctactta
//
LOCUS       DAZUMK010000313          401 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.343, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000313 DAZUMK010000000
VERSION     DAZUMK010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.343"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>401
                     /locus_tag="ACG808_003028"
     rRNA            <1..>401
                     /locus_tag="ACG808_003028"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          125 a           87 c          117 g           72 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt c
//
LOCUS       DAZUMK010000314          379 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.345, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000314 DAZUMK010000000
VERSION     DAZUMK010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.345"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>379
                     /locus_tag="ACG808_003029"
     CDS             <1..>379
                     /locus_tag="ACG808_003029"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5048551.1"
                     /translation="KLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLPE
                     HELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYLV
                     NNEYIFKTKFSTNKKKGYAKEKAI"
BASE COUNT          164 a           33 c           65 g          117 t
ORIGIN      
        1 taaattgatt tttgaatttt tgaaaaaaga aagaaatgct aatgcagtta ttttagaccc
       61 tcataaaaat aatccaagag caataagggc ataccaaaaa tctggtttta gaattattga
      121 agatttgcca gaacatgaat tacacgaggg caaaaaagaa gattgttatt taatggaata
      181 tagatatgat gataatgcca caaatgttaa ggcaatgaaa tatttaattg agcattactt
      241 tgataatttc aaagtagata gtattgaaat aatcggtagt ggttatgata gtgtggcata
      301 tttagttaat aatgaataca tttttaaaac aaaatttagt actaataaga aaaaaggtta
      361 tgcaaaagaa aaagcaata
//
LOCUS       DAZUMK010000315          356 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.347, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000315 DAZUMK010000000
VERSION     DAZUMK010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.347"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>356)
                     /locus_tag="ACG808_003030"
     rRNA            complement(<1..>356)
                     /locus_tag="ACG808_003030"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           79 a          105 c           87 g           85 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcgg
//
LOCUS       DAZUMK010000316          343 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.348, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000316 DAZUMK010000000
VERSION     DAZUMK010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.348"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            120..308
                     /locus_tag="ACG808_003031"
     CDS             120..308
                     /locus_tag="ACG808_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048552.1"
                     /translation="MLKKTMLVLGSISLGVLGFSPMLLSVIGKNNSKGELKLNPNYYN
                     SPNEPMFISEKQNDGQDK"
BASE COUNT          126 a           60 c           50 g          107 t
ORIGIN      
        1 ttctttattt aaaactttgc aacagaacca tttttacaat ttaaacattt gacaaactat
       61 agaacagtac ttaaaatgtg tatacaagtg acagcgcttt aaaaaaagag gtgcagccta
      121 tgttaaaaaa aactatgttg gtactaggtt ctatttctct aggagtatta ggttttagcc
      181 ccatgctatt atccgtaatt ggaaaaaata attccaaagg ggaacttaaa ttaaatccaa
      241 attactacaa tagtcctaat gaacctatgt ttatttcaga aaaacaaaac gacggtcagg
      301 acaaataatc ctgccgtcgt tttgctctaa cttctaccag tac
//
LOCUS       DAZUMK010000317          335 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.349, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000317 DAZUMK010000000
VERSION     DAZUMK010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.349"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>335
                     /locus_tag="ACG808_003032"
     CDS             <1..>335
                     /locus_tag="ACG808_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5048553.1"
                     /translation="FPSRSRFTRLSSNLAYTIKIIRYFFIKKLTKGELVGIIDSFPSP
                     LCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLKIHMIVTKTGFPITYSITNPGVHDVK
                     VLETLSEEA"
BASE COUNT          115 a           62 c           55 g          103 t
ORIGIN      
        1 ctttcctagt aggagtcgat tcactcgctt aagttcaaac ttagcttaca ctatcaagat
       61 tattcgatac tttttcatca agaagctcac caaaggtgaa ctagtcggaa ttatagatag
      121 ttttcctagt ccattatgta aaccagttag aaatagacaa gcaaaactat tgaatcaaat
      181 agccaaagtt ggctataact caacaaaaaa atcttatttt tatggtctta aaattcatat
      241 gatcgttact aagactggct ttccgattac ttactcaatc acgaatccag gtgtccatga
      301 cgtgaaagtg ttagagactt tatcggaaga agcga
//
LOCUS       DAZUMK010000318          332 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.350, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000318 DAZUMK010000000
VERSION     DAZUMK010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.350"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>332
                     /locus_tag="ACG808_003033"
     CDS             <1..>332
                     /locus_tag="ACG808_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048554.1"
                     /translation="IILSFLGKVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAE
                     YTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQ
                     GLYGWNVT"
BASE COUNT          103 a           53 c           78 g           98 t
ORIGIN      
        1 attatacttt ctttcttagg gaaagtacaa atggaagtga ttagtgcact gttgcaagaa
       61 aagtatcatg tggagataga actaaaagag cctacagtca tttatatgga gagaccgtta
      121 aaaaatgcag aatataccat tcacatcgaa gtgccgccaa atcctttctg ggcttccatt
      181 ggtttatctg tatcaccgct tccgttggga agtggaatgc agtatgagag ctcggtttct
      241 cttggatact taaatcaatc atttcaaaat gcagttatgg aagggatacg ctatggttgc
      301 gaacaaggat tatatggttg gaatgtgacg ga
//
LOCUS       DAZUMK010000319          325 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.351, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000319 DAZUMK010000000
VERSION     DAZUMK010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.351"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          135 a           47 c           41 g          102 t
ORIGIN      
        1 atatttatta aatcaatttt tttcattaga tatttctcct ttattaaatc tgaagtactt
       61 atattaaaat aattctaaac tatttacctc aaaaagtgca ataaattatt tctgaacaat
      121 aaaatgatag tcaaaacaaa aaaatattgt taatcgttca gagccatccg agagtgtaaa
      181 atattttgtg taaatgaaaa aatccataca aaaaaggaag gcgcttctgt agaataaagt
      241 taacgacaac caattcacag aaaagaggac ttccctatga atgattttac tacagaaatt
      301 gtgcaaactc tagtcactaa aggcg
//
LOCUS       DAZUMK010000320          324 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.352, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000320 DAZUMK010000000
VERSION     DAZUMK010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.352"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>324
                     /locus_tag="ACG808_003034"
     rRNA            <1..>324
                     /locus_tag="ACG808_003034"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           98 a           62 c           94 g           70 t
ORIGIN      
        1 gaacccacgt acgttgaaaa gtgcggggat gaggtgtggg tagcggagaa attccaaacg
       61 aacttggaga tagctggttc tctccgaaat agctttaggg ctagcctcgg aattgagaat
      121 gatggaggta gagcactgtt tggactaggg gcccatctcg ggttaccgaa ttcagataaa
      181 ctccgaatgc cattcattca tatccgggag tcagactgtg agtgataaga tccatagtcg
      241 aaagggaaac agcccagacc accagctaag gtcccaaaat atatgttaag tggaaaagga
      301 tgtggggttg cacagacaac tagg
//
LOCUS       DAZUMK010000321          302 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.355, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000321 DAZUMK010000000
VERSION     DAZUMK010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.355"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           98 a           46 c           47 g          111 t
ORIGIN      
        1 aattatgaat aattatgtta atcttccgaa atttttttcc aaaattaagg gatcaagctg
       61 gtgttataga ttgtttaagg ttggcaaaaa aataacttct ttttaaacaa ttatagaaaa
      121 ccgatactct gttaagttgg ttgacacggt gtcgattttt atgactttct tattctgaaa
      181 cttcactaac ggacagtacc taaaaagtct atagtatgga cgtttacgag tactatttta
      241 tcaaaaatgc tgctcatttc aacttatatg ttttagtgaa ctacccccac ttagcttgga
      301 ta
//
LOCUS       DAZUMK010000322          280 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.357, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000322 DAZUMK010000000
VERSION     DAZUMK010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.357"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>280)
                     /locus_tag="ACG808_003035"
     CDS             complement(<1..>280)
                     /locus_tag="ACG808_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5048555.1"
                     /translation="IHEKLALKGITISVPPRKNMDKSEKLDHSLLGKQRKTVETVFSS
                     LEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT           82 a           52 c           48 g           98 t
ORIGIN      
        1 atatcctaaa gaatatctca aagttccttc aaaacgtcgt tgtgctcgac ttaatagaac
       61 actgtaagcc agtaatatgc tttcaaatct actctctagc cctttaacag aacgtgagtt
      121 gaaattttga caacctaatt tttctaagga agaaaaaaca gtctcaactg tttttctttg
      181 ttttccaagt aagctgtggt ctagtttttc tgatttatcc atgtttttac gtggcggaac
      241 ggatatagta atgcccttaa gggcaagctt ttcatggatt
//
LOCUS       DAZUMK010000323          268 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.359, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000323 DAZUMK010000000
VERSION     DAZUMK010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.359"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..172)
                     /locus_tag="ACG808_003036"
     CDS             complement(<1..172)
                     /locus_tag="ACG808_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFS5048556.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFL"
BASE COUNT          107 a           52 c           39 g           70 t
ORIGIN      
        1 ccaagaaccc atctgctgaa atatgaaaat ttttgaatgc tacgacattc gccattacag
       61 gtatgtaaaa gaaaaaaatc atccaaagca tacccggtac agccatcagg acaagtgctt
      121 tataacgcca tagattcata aaaaaatgat tgaacccctt tcgttttttc atctaaaaac
      181 ccctttcgat tcattcgcaa cgtaattata gcgctttcaa taatgagaat aaataaacaa
      241 agtataggga aaatgaaaca aagtatag
//
LOCUS       DAZUMK010000324          262 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.360, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000324 DAZUMK010000000
VERSION     DAZUMK010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.360"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>262
                     /locus_tag="ACG808_003037"
     CDS             <1..>262
                     /locus_tag="ACG808_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS5048557.1"
                     /translation="VITLVERLSKAIIALKPEGRKAVDIENSINEWLQSVPKNLFKSI
                     TFDCGKEFSNWKSISNTNDIDIYFADPGTPSQRGLNEHSNGLL"
BASE COUNT           88 a           54 c           46 g           74 t
ORIGIN      
        1 cgtcatcaca ctcgttgagc gactttcaaa agccattatc gccttgaaac cagaaggccg
       61 taaggcagtt gatattgaaa attcgattaa tgaatggctt caatccgtac ccaaaaatct
      121 tttcaaatca attacctttg attgtggaaa agaattctct aattggaaaa gtattagtaa
      181 tacgaatgat attgatattt atttcgcaga cccaggaacg ccttcccaac ggggattaaa
      241 tgaacattca aacggactcc tc
//
LOCUS       DAZUMK010000325          248 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.361, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000325 DAZUMK010000000
VERSION     DAZUMK010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.361"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(44..>248)
                     /locus_tag="ACG808_003038"
     CDS             complement(44..>248)
                     /locus_tag="ACG808_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HFS5048558.1"
                     /translation="KVEFVHQYQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQI
                     RQQRLAKRILDNERGSDTSGEAA"
BASE COUNT           63 a           62 c           49 g           74 t
ORIGIN      
        1 atcctcggat ctgattttct ccggcagaag cacaatcatt caattacgct gcctctccag
       61 aggtatcaga tccgcgctca ttatcaagga ttcgcttcgc caatctctgt tgtcgaattt
      121 ggatcggtgt ttcatacgcc aacgtgccat gtaagcgtag atagttccac caatgcacat
      181 aatcaaacaa ttctagacgt agctgtgcca gtgtctcaaa ttggtattga tgcacgaatt
      241 ctactttg
//
LOCUS       DAZUMK010000326          244 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.denovo.363, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000326 DAZUMK010000000
VERSION     DAZUMK010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.denovo.363"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>244)
                     /locus_tag="ACG808_003039"
     rRNA            complement(<1..>244)
                     /locus_tag="ACG808_003039"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           52 a           81 c           49 g           62 t
ORIGIN      
        1 ttggacagac atttccgatc gtctgcattc cttagcctcc tgcgtccctc cattgctcaa
       61 acaaaaacaa cgggtacagg aatatcaacc tgttatccat cgcctacgcc tgtcggcctc
      121 ggcttaggtc ccgactaacc ctgggcggac gagccttccc caggaaacct tagtcattcg
      181 gtggacagga ttctcacctg tctttcgcta ctcataccgg cattctcact tctaagcgct
      241 ccag
//
LOCUS       DAZUMK010000327        19145 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.guided.047, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000327 DAZUMK010000000
VERSION     DAZUMK010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.guided.047"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..177
                     /locus_tag="ACG808_003040"
     CDS             <1..177
                     /locus_tag="ACG808_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016228883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HFS5048559.1"
                     /translation="ALDTDHVVDWCRNRISSVEASISRKGKNWYITVDGCIITVNAYS
                     YTIITAHKVQEQQR"
     gene            213..938
                     /locus_tag="ACG808_003041"
     CDS             213..938
                     /locus_tag="ACG808_003041"
                     /inference="COORDINATES: protein motif:HMM:NF025502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4304 domain-containing protein"
                     /protein_id="HFS5048560.1"
                     /translation="MTSREKKNKVIQQVIKPALKEAGFHSLRGTQAFSIHKEACTIAV
                     NIQSSQFNSNVTGFSFWLNIVVDTPDLSDEQLQDVTFFKSFTEACFLPHQGRLHPLRD
                     IRGYVIDGYKNYKPMDTPYEKIQAIIANDMQQYIIPGLSEIDSIADYQECVALWQEES
                     KSQFVRLVDYFSEAHMLTLPFQKDTWIGTVNMKELVEKKKTLCLTTDEIMQNKSIYCK
                     VRELSTHPQEDTWPLICMTLSAE"
     gene            1165..1980
                     /locus_tag="ACG808_003042"
     CDS             1165..1980
                     /locus_tag="ACG808_003042"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS5048561.1"
                     /translation="MDHSNSKIVFDEKLNIEVLTLTGMIESLPPHFHDYYELGYIESG
                     SRRVICQGKEYTIGKNDLLLFNPKDNHTCLELKKELLDFRCFHITTERMEELTLECLG
                     FAICPYFEPQIVYQSDLIPQITELIDLIENGSYCDLQKEELLYMIIGDLVADYSQPLE
                     CEQIEASDIVERACEYIERHYASNISLCDLSTFTGFSKYHFIRMFTREKGISPYRFIE
                     CTKMTEAKKMLKAGEDLSNITYQLGFSSQSHFTNFFKKYAMVTPKQYSKLYNA"
     gene            2091..2534
                     /locus_tag="ACG808_003043"
     CDS             2091..2534
                     /locus_tag="ACG808_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008689502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFS5048562.1"
                     /translation="MLLNVQQRQALIKYLLSQEKKDTDSADSPCDSFQIHDPQAEVGG
                     VFTEIEMGELYFCLEQRLVRVRGQVIELTAKEFDILALLLTHPKRVFTYELIMELVWN
                     EDYTFYSKKAVSNHMSNLRKKLKVTPDALDYIKNIVGVGYKFEAP"
     gene            2708..3406
                     /gene="vanR-D"
                     /locus_tag="ACG808_003044"
     CDS             2708..3406
                     /gene="vanR-D"
                     /locus_tag="ACG808_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-D"
                     /protein_id="HFS5048563.1"
                     /translation="MNEKILVVDDEKELADLVEVYLKNDGYTVYKFYNGKDALKCIES
                     VELDLAILDIMLPDVDGFQICQKIREKFYFPVIMLTAKVEDGDKIMGLSVADDYITKP
                     FNPLEVVARVKAQLRQYMRYKQPSLKQEAECTEYDIRGMTISKSSHKCILFGKEIQLT
                     PTEFSILWYLCERQGTVVSTEELFEAVWGERYFDSNNTVMAHIGRLREKMKEPSRNPK
                     FIKTVWGVGYTIEK"
     gene            3396..4541
                     /gene="vanS"
                     /locus_tag="ACG808_003045"
                     /pseudo
     CDS             3396..4541
                     /gene="vanS"
                     /locus_tag="ACG808_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_028530133.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS"
     gene            4641..5732
                     /gene="vanY-D"
                     /locus_tag="ACG808_003046"
     CDS             4641..5732
                     /gene="vanY-D"
                     /locus_tag="ACG808_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_049898469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transpeptidase-like D-Ala-D-Ala carboxypeptidase
                     VanY-D"
                     /protein_id="HFS5048564.1"
                     /translation="MWKGVKRNVERQNNNENQYGRNRRKDKRRKLFFYRAACAMLGLL
                     IVCVIFGAVYFLRESKDPALPSKENTKTSKDYSFLADGQSEEESPASEPAISNRANAI
                     DLNIIAANAIVVNKDTDALLYQKNGTDRIAPASTAKMITALTVLEYCSPEDEMRVGAE
                     IEMIHSDSSTAWLMKGDTLTVRQLLIALMLPSGNDAAYTLAVNTGKTIAGDNSLSNQQ
                     AIQIFMDKVNEKAKAIGVIDSNFVVPDGYDAEGQYTTAYDLAIIAKACLEDPIISEIV
                     ASNTSYERWPNGREVTYNNSNELLDPNSPYYRPEVIGLKTGTSSLGGACVVSAAVIDG
                     ETYICVVMGSTKESRFQDSVDILDKIKAR"
     gene            5752..6723
                     /gene="vanH-D"
                     /locus_tag="ACG808_003047"
     CDS             5752..6723
                     /gene="vanH-D"
                     /locus_tag="ACG808_003047"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023043160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-D"
                     /protein_id="HFS5048565.1"
                     /translation="MNKKMDITVFGCESDEMEVFQKISYELGVTATLIKDSISESNAG
                     LADGCCCVSVSHKAELSEPILLALKNAGVKYISTRSIGFNHIDIQAAGLLGMVVGTVE
                     YSPGSVADYTVMLMLMLMRGTKSILRETQRQNYCLNDLRGKELRDMTVGVLGTGRIGQ
                     AVMERLKGFGCKVLAYDRNQKAGTDYVSFHELLKKSDIVTLHIPLAEDTRHMIGCEEL
                     EMMKQEALLINTGRGALVDTAALVEALKGQKIGGAALDVLEGEEGIFYHDCTQRTIEH
                     PFLSVLQGMPNVIVTPHTAYHTDRVLVDTVSNTIRNCLNFERSLGNV"
     gene            6716..7747
                     /gene="vanD"
                     /locus_tag="ACG808_003048"
     CDS             6716..7747
                     /gene="vanD"
                     /locus_tag="ACG808_003048"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023043159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanD"
                     /protein_id="HFS5048566.1"
                     /translation="MYKLKIAVLFGGCSEEHDVSVKSAMEVAANINKEKYQPFYIGIT
                     KSGAWKLCDKPCRDWENYAGYPAVISPDRRTHGLLIQKDGGYESQPVDVVLPMIHGKF
                     GEDGTIQGLLELSGIPYVGCDIQSSVTCMDKSLAYMVVKNAGIEVPGFRVLQKGDSLK
                     AETLSYPVFVKPARSGSSFGVNKVCRAEELQAAVTEAGKYDCKILVEEAVSGSEVGCA
                     ILGNENALMAGEVDQIELRHGFFKIHQEAQPEKGSENAVIRVPAVLPDEVRERIQKTA
                     MKIYRILGCRGLARIDLFLREDGCIVLNEVNTMPGFTSYSRYPRMMTAAGFTLTEILD
                     RLIELSLRR"
     gene            7753..8361
                     /gene="vanX"
                     /locus_tag="ACG808_003049"
     CDS             7753..8361
                     /gene="vanX"
                     /locus_tag="ACG808_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023042861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX"
                     /protein_id="HFS5048567.1"
                     /translation="MKKNFAFLDEMIPGIRWDAKYATWDNFTGKPVDGYMVNRVMGTK
                     ELGVALRKAQKMAEKLGYGLLLWDGYRPQCAVDCFLNWTSQPEDNLTKKRYYPNIKRN
                     EMVAKGYVASKSSHSRGSTVDLTIFHLNSGMLVPMGGDFDYMDERSHHTASGLSEEES
                     KNRQCLRYIMESSGFEAYRYEWWHYVLVDEPYPDTYFDFCIA"
     gene            8522..9433
                     /locus_tag="ACG808_003050"
     CDS             8522..9433
                     /locus_tag="ACG808_003050"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5048568.1"
                     /translation="MKSNRMFGILCILLERKKITAQELAEYFEVSVRTIHRDLLDLSS
                     AGFPVTTQQGIGGGISLLPNFKYSKSALNKEDIDLIVSGIQGFASIDESSKIKTLLAK
                     LRLGQEDKLLLENDIIIDFTSWNHKSTTIEKIKIIRSAIASRRLLELEYYSSNGYSKR
                     TVEPYKLIFKEEYWYLFAYCTQRQDFRVFKLNRISKLLLCEQIYTERTDYEIPVLQSA
                     FSNGVGQLVTVRMDKSYEFLAIDFFGQSNIREENNSLYISFYTEYPEWIVSTFASLGD
                     KAEIIEPKTLRDDIKAFLEQARKQYDK"
     gene            9576..10217
                     /locus_tag="ACG808_003051"
     CDS             9576..10217
                     /locus_tag="ACG808_003051"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5048569.1"
                     /translation="MTDTKEKILAVALQLFAKDGYEAVSVSKIAEKLGITKGALYKHY
                     ENKKDIFNSIVARMSQSDHEIARKYGVPENVFDCAPEQYEKIGLKNLKNFAVSQYRFW
                     TSDEFAADYRKMLTLEQYRNPELNKLYQDSLVRGPVLYLKDIFREMIRCGVLKENDPY
                     QLAIEFFSPLFLLINMSDENESYVDIEKRLIKHIDFFIESHTKERENKNEIYS"
     gene            10201..10917
                     /locus_tag="ACG808_003052"
     CDS             10201..10917
                     /locus_tag="ACG808_003052"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HFS5048570.1"
                     /translation="MKYIRSQKYNIPELQAKIMGPNPVKLEEELLLHCKISQNAIVCD
                     LGSGQGLTSLFLVKEYGFTVYATDLWSDPEDNRKFFRKMGLSDEQIIPVKADATALPF
                     EEEFFDAIISTDSYNYFGRDPKFLDDKLLPYLKHGGYVYIAIPGMKKDCHSNLPPELL
                     LSWTPDQLDYMHDIMYWENIIRHCKGARVISIHEMESNEEVWNDWLKQENEYAISDRK
                     AMEAGGGKYLNFISIVLQRL"
     gene            11246..11689
                     /locus_tag="ACG808_003053"
     CDS             11246..11689
                     /locus_tag="ACG808_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008689502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFS5048571.1"
                     /translation="MLLDARQRQELVNYLLGKEKQNGGSADILCIRSGAYELLTENEG
                     IFTEIHMGDLYFCLEQRLVRVDGQVVELTAKEFDILALLITHPQRVFTYELIMELVWD
                     EAAACYSRKAVSNHMSNLRKKLKITPDGLEYIKNIVGVGYKFEIP"
     gene            11881..12258
                     /locus_tag="ACG808_003054"
     CDS             11881..12258
                     /locus_tag="ACG808_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5048572.1"
                     /translation="MEIIISSNTSKPIYEQITSQIKAMIMSGELQTGDPIPSMRALAK
                     SIHVSVITVQKAYEDLQRDGFIETTVGRGSFVAAQNKDFYQEEQQRLAEEHLQEAADI
                     GRTSGISLGKLVELLTMFYQEEE"
     gene            12261..13121
                     /locus_tag="ACG808_003055"
     CDS             12261..13121
                     /locus_tag="ACG808_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005933713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5048573.1"
                     /translation="MDTILQVENLTKQYEGFQLDHVSFDLPKGTIMGLIGENGAGKST
                     TINAILDLIKKDDGTVTFWGQELSSSKQLKEDIGVVFDGVNFYETLTPAKVGKISGSA
                     YKQWDEYLYRDYLKRFGLPLDKEIKTLSKGMKMKLCIAVALSHKPKLLILDEATSGLD
                     PVMRDDILDVFLDFVQDENHSILMSSHITTDLEKVADYITFIHQGKVLFCKTKDELRY
                     KYGIIRCGAAVFDQIDKAEILAYRKDDYQWNVLVADKEKARKKYKAAVVDDATIDDIL
                     LLYVKGERAK"
     gene            13118..13765
                     /locus_tag="ACG808_003056"
     CDS             13118..13765
                     /locus_tag="ACG808_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HFS5048574.1"
                     /translation="MKSLILKDLYNIGHNTKSMLFILAVFAVAFIPTSGVGAYIFICG
                     ILCSMMIVTTFSFDDNSKWARYAMIMPISKRDLVIGKFIVLAIFCAIGSVFGLAVGLI
                     GGGIAGKITFSPAAIVELLFLTLAATVISLIFGSMSISLVFKFGAEKGRVLLLVSFLI
                     PAAICFGAYKLLAILGVELTDQKVFILLCCSPLIALVWSYGMYQISYRIFLKQEL"
     gene            13782..13967
                     /locus_tag="ACG808_003057"
     CDS             13782..13967
                     /locus_tag="ACG808_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HFS5048575.1"
                     /translation="MKKYEYVNIEYSAKGVMFSYVEKHREIIDSYAEKGFRYVGFVPT
                     EVGANGCIRKIDLVFEK"
     gene            complement(14331..14933)
                     /locus_tag="ACG808_003058"
     CDS             complement(14331..14933)
                     /locus_tag="ACG808_003058"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5048576.1"
                     /translation="MPRQFKQARLINKLKMTEAAEKLGISQPTLSAWEGERKSPSIDG
                     LEKMSVLYGVTTDFLLGRSEQGISVQSVPIAPETLPIFHGKPVWSAEYGWMLVDAGNH
                     TLLLPNGQTVPFADAKRLFTLPQLFSEPSLPSGKPLILSEIQQFTRIWLEPISPDSNL
                     RTELRGWYQVKKHFVQNEYGNRFYLDTYRAKWLAFHPEQQ"
     gene            complement(14947..16308)
                     /locus_tag="ACG808_003059"
     CDS             complement(14947..16308)
                     /locus_tag="ACG808_003059"
                     /inference="COORDINATES: protein motif:HMM:NF016232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5048577.1"
                     /translation="MPEMQIIEWKEIWKDDYLAWICGFANAQGGTLYIGKNDNGETVG
                     LSNSHKLLEDLPNKIRDAMGIIVPVNLHTDGETEYIEISVQPYPIAISYKGVYYYRSG
                     STNQKLTGLELESFLLRKQGATWDNLPLPSFTISDIDNGVVKRFIKWAAKKGRIDESA
                     LDEQKETLMKKLHLMNNDYLTNAAMLLFANDPEEWQLGAFTKIGYFESDSELRYQDEI
                     HGSLLDQIDRIIETIHLKYMKAKITYEGMQRIERYFVPDAALREALLNALCHKDYARG
                     IPIQVSVYNDRLYIANCGRLPENWSAEKLMEKHSSEPFNPNIAHVYYLAGFIESWGRG
                     IEKICDACREDGVPLPEYDITGNSVMIKFIAPKDRIVYGPGETANGTVNGTVNGTVNL
                     SDKEKEILALLSEDPAYTYQNLADKLNIGRKAVFGRIKNLKEKGIIERIGSDKNGYWQ
                     INE"
     gene            complement(16301..16507)
                     /locus_tag="ACG808_003060"
     CDS             complement(16301..16507)
                     /locus_tag="ACG808_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFS5048578.1"
                     /translation="MKFISTTEAGKKWGLSSRRVGILCSEGRIPGTQKTGNTWLIPED
                     AEKPADARIKSGKYIKKCEEQSNA"
     gene            16730..17587
                     /locus_tag="ACG808_003061"
     CDS             16730..17587
                     /locus_tag="ACG808_003061"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5048579.1"
                     /translation="MYEEVGISSNNVPITLSIWYTERRSPTIVFLPGTMSHPLMYENF
                     LCGLSERGFNIIGVHYLSHGKSPKIKKNYTMEDMLHNVYDATTYGIENFGENIAVMGS
                     SQGGILAAMAAGKDTRLKAVFPHNIMLTTLKETMSLTKYPAWTHRFMGLIQWGFRVGG
                     KLLPNYKVPGDAYLDYDRVFYSEQAKQDCLNDPMLLPYYPLRFVASLFNADLSCLENG
                     SIQCPVILITAKGDPLFSFDYTSKVFDLIHAPQKELLTLELNRHMIFNEDTDTTINAL
                     EPTLHKYLG"
     gene            17714..18247
                     /locus_tag="ACG808_003062"
     CDS             17714..18247
                     /locus_tag="ACG808_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5048580.1"
                     /translation="MIEGEKLFALHGLKKVTVDDLVAAVNISKGSFYAFYPSKEHLYV
                     EINFRLQKELFESIETTIKKKKYKNHRDLAKDAIKLSLTGLITSPILSQIDLSIMDYL
                     QRKLSPDIFESHMHNDIRILEILEDLGVVFLVPHTVIIKSLYSVLSCLEQFKEDEELN
                     MIQNLLVNGIIQQVVAE"
     gene            18350..>19145
                     /locus_tag="ACG808_003063"
     CDS             18350..>19145
                     /locus_tag="ACG808_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5048581.1"
                     /translation="MIDVKKLYFSYTEKPFVEDVSFHVGRGEIFGFLGPSGAGKSTIQ
                     KVLTGLNTKYKGSVKVAGIEIRERTNRFYENIGVDFEFSTCYEKFTARQNLAYFASLY
                     EKQPRSIDELLHMVGLENDGDKKVADFSKGMRSRLNFIKALIHDPDILFLDEPTSGLD
                     PTNNRLMKDIILAEKKRGKTIIITTHNMYDATELCDQVAFIVAGKVSALDSPHNLIMS
                     RGAAKIQYTYYDNGEKTGECLLDRTTEDKMLKNLISENRLLSIHSSE"
BASE COUNT         5800 a         3495 c         4486 g         5364 t
ORIGIN      
        1 gctttggata ccgatcatgt tgttgattgg tgcagaaata ggatatcttc tgttgaagca
       61 tccatatcga gaaaaggaaa gaactggtat ataacggttg acgggtgtat cattacggtc
      121 aacgcttata gctatacgat catcacggcg cataaggtac aggaacagca aagatagcaa
      181 taaagaactg acagaaagag cggtacaaga atatgacttc acgagaaaag aaaaataaag
      241 taattcaaca agttatcaaa ccggcgctca aagaagcagg ttttcacagc cttcggggaa
      301 cacaggcttt ttccatccat aaggaagcct gcacaatcgc cgtaaatatt caaagctccc
      361 aatttaacag caatgttact ggattttcct tttggctgaa tatcgtagta gatacgcctg
      421 acctttcaga tgaacaactg caagatgtta cttttttcaa atcttttaca gaagcttgct
      481 ttttgccgca tcaaggcagg ttacacccgc tccgtgatat acggggttat gtcattgacg
      541 gttataaaaa ctacaagccg atggataccc cctatgagaa gattcaagcc atcattgcaa
      601 atgatatgca gcagtatatc attccggggc tatccgagat tgacagcata gcagattatc
      661 aagaatgcgt tgcgctgtgg caagaagaat caaaaagtca gtttgtacgt ctggtagatt
      721 acttttcgga agcgcatatg ctgacattac cgtttcaaaa agatacttgg attggcaccg
      781 taaatatgaa agagctcgtt gagaagaaaa agacactatg ccttaccaca gatgaaatca
      841 tgcaaaataa atcaatttat tgcaaagtgc gtgaattatc aacgcatccg caagaggaca
      901 catggccatt gatttgcatg acgctctctg cagaataaga tgatttgaaa tgtgcggaac
      961 ttcctttgag aaggaggagc aaataatgaa gcgttatgga aatgattcgg gatcattgcc
     1021 tccgtaacgc ttgttcgtat ttttatacag gtataaaagt acgatattcg acagacctta
     1081 acacaaatag caacattatg caagaaaaag aaatacgaat acactataat gatattattg
     1141 gagtaatttt tgcggaggtg acttatggat cattccaaca gtaaaattgt ttttgacgaa
     1201 aaactgaata tagaggtatt aacactgaca ggaatgattg aaagtttgcc tccgcatttt
     1261 catgattact atgaattagg ctatatagag agcgggagcc gacgagtaat atgtcaaggt
     1321 aaggaataca ccataggaaa aaatgatctg ctccttttca acccgaaaga taatcatact
     1381 tgtttggaat tgaaaaagga actgttggat tttagatgtt tccacatcac tactgaacgc
     1441 atggaagaat tgacattgga atgtttagga tttgccatat gtccgtattt tgaaccgcag
     1501 attgtttatc agagtgatct tattccgcaa atcacagagt tgattgactt aattgagaat
     1561 ggttcatatt gcgatttaca aaaggaagaa ttgctttata tgatcattgg cgatttggtt
     1621 gcagattatt ctcaaccatt ggagtgcgaa caaatagaag cttcggatat tgtagaacgt
     1681 gcttgtgagt atattgaaag gcattacgcc tcaaacatat ctttatgcga tttaagtacc
     1741 tttacgggtt tcagcaagta ccactttatt cggatgttta cgagagaaaa gggaatatct
     1801 ccttatcgct ttattgaatg tactaaaatg actgaagcca aaaaaatgct gaaggccgga
     1861 gaagatttat caaatattac ctatcagctt ggttttagca gccaaagcca ctttaccaat
     1921 ttttttaaga aatacgccat ggttactcca aaacaatata gtaagcttta taacgcttaa
     1981 aatgtatttt ttgttgagta tagatccata ctaacaggag atgatgatat gggaaattag
     2041 ccgtgataga aatctctgag tcaggagata aaattggaga aaaactgctt atgctgttaa
     2101 acgtccagca gcggcaggca ttaataaaat accttcttag tcaagaaaag aaggataccg
     2161 attcagctga tagcccatgt gacagctttc aaatccatga tccgcaggcg gaggtcggtg
     2221 gagtattcac agagatcgag atgggagagc tatacttttg tttggagcag cggcttgtac
     2281 gtgtgagagg acaagtgatt gagttaacag caaaagaatt tgatattctt gcgctattgc
     2341 tcacacatcc caaaagggta tttacttatg aattgatcat ggagttggtc tggaacgagg
     2401 attatacttt ttattccaaa aaagcggtaa gcaatcacat gagtaacctg cggaagaaac
     2461 tcaaagttac accggatgct ctggactata ttaaaaacat tgttggtgtc ggctataaat
     2521 ttgaagcacc ataaaaaaca tataatttgc cattgttagc agaatagcgg taatcgtcaa
     2581 gaatctggat aaactggccc gtcagctcgc cgtgccttga cgattatttg cgattctgct
     2641 attgttgttt catatacggt tttgacacat aataaagaca gattatcgca atgtaaggag
     2701 taatgcaatg aatgaaaaaa tcttagtggt tgatgatgaa aaagaattgg ccgacttagt
     2761 tgaagtatat ctgaaaaacg atggatatac cgtttataaa ttttataatg gcaaggatgc
     2821 actaaagtgt attgaatccg tggaactgga tttagccata ttggatatca tgcttccgga
     2881 tgtagacggg tttcagatct gccagaaaat ccgggaaaag ttttacttcc ctgttatcat
     2941 gctgacagca aaagtggagg acggggataa aatcatggga ctgtctgtgg cagatgatta
     3001 tattacgaag ccgtttaacc cgttggaagt ggttgcgagg gtaaaggcgc agctaaggca
     3061 gtacatgcgg tacaagcagc ccagcttaaa gcaagaggct gaatgcacag aatacgatat
     3121 cagagggatg acaattagca agagcagcca taagtgtatc ttgtttggaa aggagattca
     3181 gctgacgcca acggaatttt cgattctttg gtatctgtgc gagcgtcagg ggacggttgt
     3241 ttctacggag gaattatttg aagcagtatg gggggaacgg tattttgaca gcaataatac
     3301 cgtgatggcg catatcggac gtctccggga aaaaatgaag gaaccgtcaa gaaatccgaa
     3361 atttataaaa accgtgtggg gagtgggata taccattgaa aaatagagat aaaaccagtc
     3421 atgaagatga ctatttactt tttaaaaaca gactgtccct taaaatactg ctgataatgg
     3481 catgctccat tctaattata gcggcagtgt atctattcgt tttaaaagat aattttgcaa
     3541 atgtggtggt agccatttta gatagcttta tctatcatga tcgggatgag gcggtggctg
     3601 tttatctgag aaccttttag gcgtatgaga tatggctttt tctgatagcg gttatgggtg
     3661 tgttttttat gatctttcgc cgttatctgg acagtatctc aaagtatttt aaggagatca
     3721 accgggggat tgataccctg gtgcatgaag ataccaatga tatcgccctg cctccggaac
     3781 tggcttcgac cgaaagaaaa atcaattcca tacggcatac actgacgaaa cggaaaacgg
     3841 acgccgagct tgcggaacag cggaaaaacg accttgttat gtacctggct catgacttga
     3901 agactccgct ttcatcggtt atagggtatt taaacctgtt aagggatgag aatcaaatct
     3961 ccgaggaact ccgggaaaaa tatctgtcca tttcactgga taaagctgaa cgtctggaag
     4021 agctgatcaa tgagtttttt gaaattacga ggtttaatct ttcaaacatc acgcttgtgt
     4081 acagcaaaat caatctgacg atgatgctgg aacagctggg gcacgagttt aagccgatgt
     4141 tggccgggaa aaatctcaaa tgtgaatttg atgttcagcc ggacatgatg ctgtcctgtg
     4201 atgccaacaa actgcagaga gtgttcgata atgtgctgag aaatgccgtc agctactgtt
     4261 atgagaatac taccattcag gtgaatgcca ggcaggcgga agactatgtg ctcatccaag
     4321 tcataaacga aggggatacg attcccgggg agagattgga aagaatcttt gagcagtttt
     4381 accgcctgga tgtgtcacga agctcaagca ccggcggagc cggtctggga cttgcgattg
     4441 caagggagat tgtggaactg caccatggac agatcactgc ccgcagcgaa aacggtatca
     4501 ccagttttga ggttacattg cccaccgtag gaaaatcgta agaaattccg agataaaccg
     4561 tgtgttatcc ataaaataat gcgaaaaaat aaatcgctcc attctggtat tctttatatc
     4621 agagggagcg atttttttgt atgtggaaag gagttaagag aaacgtggaa cgtcaaaata
     4681 acaatgaaaa ccagtatgga aggaaccgca gaaaagacaa aagaagaaaa ttgttttttt
     4741 acagagcagc atgtgccatg ctcggtctgc tcatagtctg tgtaattttt ggagctgtgt
     4801 attttctgag agagagtaaa gatcccgctc ttccatccaa agaaaataca aagacaagca
     4861 aggactattc atttctggcc gacggccaga gtgaggagga gtctccagct tcggagccag
     4921 ccatatccaa ccgggcgaat gcgattgacc tgaacattat agcagcaaat gccattgtgg
     4981 tgaataaaga caccgatgcg ttattgtatc aaaaaaacgg cacggacaga attgcgccgg
     5041 ccagtacagc caaaatgatt acagcgttga ccgtgcttga atattgttct ccggaggatg
     5101 agatgagagt cggtgcggag attgaaatga tccatagtga ttcgtcaacc gcatggctta
     5161 tgaaaggcga taccctgact gtcaggcagc tcctgattgc cctgatgctt ccgtccggca
     5221 atgatgcggc ctataccctt gcagtcaata ccggaaagac cattgcgggt gataatagcc
     5281 tgtccaatca gcaggccatt caaatattca tggataaggt aaatgagaag gccaaggcaa
     5341 ttggcgttat agactcgaat tttgtggttc cggatgggta tgacgccgag gggcagtata
     5401 ccacggccta tgatcttgcc atcattgcaa aggcatgttt ggaggatccc attatttcag
     5461 aaattgtggc gagcaatacg tcatatgaaa gatggccgaa cggaagggag gtcacttaca
     5521 acaattccaa tgagcttctt gatccgaaca gtccctatta tcgtccggag gtcatcggcc
     5581 tgaaaaccgg gaccagcagc cttggtggtg cctgtgtcgt ctccgcagca gtgattgacg
     5641 gagaaaccta tatctgcgta gtcatgggct ctacgaaaga aagcaggttt caggacagcg
     5701 ttgatatttt agataaaatc aaagcccggt aataggataa ggaggaaacg aatgaataaa
     5761 aaaatggata ttactgtttt tggatgtgag tcagacgaaa tggaggtttt tcaaaagatt
     5821 tcttatgagc ttggtgttac agctacactc ataaaagatt ctatatcaga aagcaatgct
     5881 ggattagctg atggatgctg ctgcgtaagc gtaagccata aagcggagct gtcagaaccg
     5941 attcttcttg cactaaaaaa tgcaggggta aagtatatca gtacccggag cattggcttt
     6001 aaccatattg atatacaggc ggccgggttg ctgggtatgg ttgttggcac ggtagaatac
     6061 tcgccgggaa gtgtggccga ttataccgtc atgctgatgc ttatgctgat gcgcggcaca
     6121 aagtcgattc tgcgtgaaac ccagaggcag aattattgcc tgaatgacct gcgcggaaaa
     6181 gaactgcggg atatgaccgt cggtgtgtta ggaactgggc gaatcggaca ggcagtcatg
     6241 gaacgcctga agggattcgg ctgtaaggta ttggcgtatg accgaaatca aaaagcagga
     6301 acggactatg tttcgtttca tgaactgctg aaaaaaagtg atattgttac actgcatatt
     6361 ccgttggcag aagatacccg ccatatgatt ggatgtgaag agctggaaat gatgaagcag
     6421 gaggcgcttc tgatcaatac aggacggggc gctttagtgg ataccgcagc attggtagaa
     6481 gcattaaaag gacagaaaat cggcggtgcc gccctggatg ttttagaggg cgaagaaggt
     6541 atcttttacc atgactgcac tcaaagaacg atagagcatc ctttcctgtc tgtcctgcag
     6601 ggaatgccga atgtcattgt tacgccgcac acagcctatc atacggatcg ggtactggtc
     6661 gataccgtga gcaataccat ccgaaattgt ctgaattttg aaaggagtct tggaaatgta
     6721 taagcttaaa attgcagtcc tgtttggagg ctgctcagag gaacatgatg tttcagtgaa
     6781 atctgcgatg gaggttgcag caaatataaa caaggaaaaa taccagccgt tttatattgg
     6841 aatcacaaaa tccggcgcat ggaaactatg cgataagccc tgccgggact gggaaaacta
     6901 tgcgggatac ccggctgtga tttctccgga cagaaggacc catggcctgc tgatacaaaa
     6961 ggatggcgga tatgagagcc agcctgtaga cgtggtgctt ccgatgattc atggaaaatt
     7021 tggcgaggac ggaaccatac agggtctgct tgagctgtcc ggcattcctt atgtgggatg
     7081 cgacattcaa agttctgtaa cctgtatgga taagtcgctc gcttatatgg ttgtgaaaaa
     7141 tgcgggaatt gaggtacctg ggtttcgagt tctacaaaag ggggacagcc tgaaagcaga
     7201 gacactctcg tacccggtct ttgtaaaacc tgcccgttcc ggctcctctt ttggcgtgaa
     7261 taaggtatgc cgggcagagg aactgcaggc agcggtcaca gaggcgggca agtatgactg
     7321 caaaatattg gttgaggagg ccgtttccgg gagtgaggta ggctgtgcca tactgggaaa
     7381 tgaaaatgct ctcatggctg gcgaggtgga tcagattgag ctgagacacg gtttttttaa
     7441 gattcatcag gaagcacagc cggagaaagg ttctgaaaat gcagttatca gagttccagc
     7501 tgtcttaccg gatgaggtaa gagaacggat tcagaaaaca gcaatgaaga tttaccggat
     7561 acttggctgc cgaggattgg ctcgtattga tctgtttttg cgggaggacg gctgcattgt
     7621 gctgaatgaa gtgaatacca tgccgggttt tacttcctac agccgttatc cccgcatgat
     7681 gacagcagcc ggttttacgc ttactgaaat actggatcgc ttgattgaac tttcacttag
     7741 gaggtaactg ccatgaaaaa gaactttgcc tttttagatg aaatgattcc cggaatccga
     7801 tgggatgcta aatatgccac atgggacaat ttcaccggga aaccggtaga cggatacatg
     7861 gtaaaccgtg ttatgggaac gaaggagctg ggagttgctt tgcgtaaggc tcagaagatg
     7921 gcggagaaac tgggatatgg tttgctctta tgggacggct atcgccccca gtgcgcagtg
     7981 gattgttttc tgaattggac ttcccaaccg gaagacaatc tgacgaaaaa gcgttactat
     8041 ccaaatatca aaaggaatga gatggttgcg aaggggtatg tggcctcaaa atccagccac
     8101 agccgtggaa gtacggttga ccttacaatt tttcatttga atagcggtat gcttgttcct
     8161 atgggtggag attttgacta tatggatgaa cggtcacacc ataccgcaag cggtctgagc
     8221 gaagaagaat caaaaaaccg gcagtgtttg cgttatatca tggagagcag cggatttgaa
     8281 gcctatcgtt atgaatggtg gcattacgtc ttggtggacg agccataccc ggatacatat
     8341 tttgattttt gtatcgcctg atgaatctgc ccagatgcgc cgcagcaagc tgacggggta
     8401 tctggcgtgg aaacataagc tccgtaatct tttgccatag ataggttcac tttgtctgtt
     8461 acagcggaaa cttgacattt gatgtcttat ttctgtaaaa ttgacagagg aggtgccttg
     8521 aatgaaaagc aacagaatgt tcggtatatt atgtatttta ttggagcgga aaaaaataac
     8581 ggcacaggag ctggcggagt attttgaggt ctctgtgcgc acgatccata gggatttgtt
     8641 ggatttgtcc agtgcagggt tcccagtgac tacacagcag ggcattggcg gcggtatatc
     8701 tttgctgcca aattttaaat acagcaaatc agcactgaat aaagaggaca tagatctaat
     8761 tgtgtccggc atacaagggt ttgcaagtat tgatgagagt tcaaaaataa agacactgct
     8821 tgcgaagctg cgtcttgggc aggaagataa gctgctgctg gagaacgata tcatcattga
     8881 tttcacatcc tggaaccata agagtacgac cattgaaaaa atcaaaatca ttcgttctgc
     8941 gattgcctcc cggcgcctgt tggaactgga gtattacagc agcaatggat actccaaaag
     9001 aaccgtggaa ccctataagc tcattttcaa agaagaatac tggtatcttt tcgcctactg
     9061 cacacaaagg caggatttcc gtgtttttaa gcttaacaga atatcgaagc tgttgctttg
     9121 cgagcagatc tatacggaac ggacagatta tgaaattccg gtattgcaga gcgcattttc
     9181 aaatggcgtg gggcaattgg ttacagtcag aatggataaa tcctatgagt ttctggcgat
     9241 tgatttcttt ggccagagca atatccggga agaaaataat tctctttaca tttcctttta
     9301 tacggaatat ccggagtgga tcgtaagcac ttttgcaagc ttgggggata aagcggaaat
     9361 tatagagcca aagacgctgc gggatgatat caaggcattt ttagagcagg ccagaaaaca
     9421 atatgataaa tgagaaccag cgattctcac ttttacgata ggaaatgctt tgcacagatt
     9481 ggctcataaa aatcctcttg acaagtaaac gagcgtttac tataatatca aaaagaagag
     9541 gtaaacgatt gtttacattg taggaggtac atagaatgac tgatacaaag gaaaaaatat
     9601 tggccgttgc attgcaatta tttgcaaaag acggatatga agcagtttcc gtcagtaaaa
     9661 tagcagaaaa attgggaatt acaaaagggg cgctgtataa gcactatgaa aataagaagg
     9721 atatttttaa cagtattgtg gcgcgtatgt cacaatcgga tcatgaaata gcacggaaat
     9781 atggtgtgcc agaaaatgta tttgattgtg caccagaaca atatgagaaa atcggattga
     9841 aaaatcttaa aaattttgcc gtttcacaat ataggttttg gacttcagat gaatttgccg
     9901 ctgattatcg taagatgctc acattagagc agtatcgtaa tccagaatta aacaagttgt
     9961 atcaggatag ccttgtgcgt gggcctgttc tttatttaaa agatattttt cgtgagatga
    10021 ttagatgtgg tgtgttaaag gagaatgacc cataccaact tgcaatagaa tttttctccc
    10081 cgcttttttt acttatcaat atgtcagatg aaaatgaatc ttatgtggat attgaaaaac
    10141 ggctaataaa gcatattgat ttttttatag aatctcacac aaaggaaagg gaaaacaaaa
    10201 atgaaatata ttcgtagtca aaaatataac attcctgaat tacaggctaa aattatgggg
    10261 ccaaatccag tcaaactgga ggaggaactt ttacttcatt gcaaaatctc ccaaaacgcc
    10321 atagtatgtg atcttggcag cggtcagggg ttgacttccc tctttcttgt aaaagagtac
    10381 gggtttactg tatatgccac ggatttgtgg agcgatccgg aagataatcg aaaattcttt
    10441 cgcaaaatgg gtttgtccga tgagcagatt attccggtta aggcagatgc cacggcactt
    10501 ccgtttgagg aagaattttt cgatgcaatc atcagtacgg attcctataa ttattttggg
    10561 cgagatccta aattccttga cgataaattg ctcccatatc ttaaacatgg tggctatgtt
    10621 tatattgcta ttcccggaat gaaaaaagat tgtcatagta atctgccacc agaattactt
    10681 ctctcatgga caccagatca gcttgattat atgcacgata taatgtattg ggaaaacatt
    10741 atccgccact gcaaaggggc cagggtaatt tccattcatg agatggaaag taatgaggaa
    10801 gtatggaacg actggttaaa gcaagaaaac gagtatgcaa tcagtgatcg taaagctatg
    10861 gaggctggcg gaggtaaata tttgaatttt atttctattg ttcttcaaag attatagcaa
    10921 taaaatcaag agacggccga gtttgattat gaagaagccg gatgctgcct tgatacttca
    10981 ccgggaagag tcatgtactt ttttatgatc cggaacagta ttttaagtat ataaggccag
    11041 tgcgaaaagg gtaggggcat aaagcccagg taaggtgtca ttgcacaaaa aagatatgaa
    11101 atatgacaga acgctgtcat gtttatagtt tatcatagaa tgctgaagga cggaggggcg
    11161 taaagggaac ggaggtgata tctttgggaa attagctgta ctggaaatat ccgagccggg
    11221 agataaaatc ggagaaaagc tgcttatgct gttggacgca agacagcggc aggagttagt
    11281 aaactatctt cttggcaaag aaaagcagaa tggtggttca gctgatatcc tgtgtatccg
    11341 ctccggagct tatgagctgt tgacagagaa tgaaggtatc ttcacagaga ttcatatggg
    11401 tgatttgtac ttttgcctgg agcagcggct ggtacgtgtg gatggacaag tggtcgaact
    11461 gacggcgaaa gaatttgata ttcttgccct gctgatcacg catccccaac gggtgttcac
    11521 atacgagctg atcatggaac tggtctggga tgaggctgct gcctgttact cacgcaaagc
    11581 ggtaagcaac catatgagca atctgcggaa gaaattgaag ataacaccgg atggcttgga
    11641 atatatcaaa aatattgtcg gtgtaggtta taaatttgaa ataccataac aaagatcaca
    11701 aggattttac ttcccctgtg gtcttgtttt gtttatgctc cttttccaaa gaacagatcc
    11761 catttgttat tgttctcata agaattttgc cacctgccat taccttattg acagttgtga
    11821 ctgaagttgc tataatgtac tcgttcaata agtacattat atgacggatg gagggatcct
    11881 gtggaaatta taataagcag taatacgagt aaacccattt atgagcagat tacctcacag
    11941 ataaaagcta tgattatgag cggtgaactg cagacgggcg atcctattcc ttctatgcgg
    12001 gcattggcaa agtcaatcca tgtaagcgta attaccgttc aaaaggcata tgaagatcta
    12061 caaagggatg gttttatcga aacgacagtc gggcgtggaa gttttgtagc ggcacagaat
    12121 aaagactttt atcaggagga acagcagcgg ttagcggaag agcatttaca ggaggccgct
    12181 gatattggca gaaccagtgg aatatcgttg ggaaaattag ttgagctttt aactatgttt
    12241 tatcaagagg aggagtgact atggatacaa tcttacaggt ggaaaattta acgaaacagt
    12301 atgaaggctt tcagctggac cacgtttcgt tcgaccttcc gaaaggaacg attatgggac
    12361 tgattggcga gaatggagcc ggaaagagta caacgatcaa tgcaattctt gatctgatta
    12421 agaaagatga cggtacggtt actttttggg ggcaggaatt atcttcatcc aaacagttga
    12481 aggaggatat cggcgttgta ttcgatggag tcaattttta tgaaacattg acgcctgcga
    12541 aagtggggaa gatctctgga tccgcctata aacaatggga tgagtacctg taccgggatt
    12601 atctgaaacg tttcgggctt ccgttggata aagagatcaa aacactttcc aaaggcatga
    12661 aaatgaaatt gtgtatcgct gtggcgcttt cccataaacc gaagctgttg attttagacg
    12721 aggcgaccag cggtcttgac ccggttatgc gggatgatat tcttgatgtc tttttggatt
    12781 tcgtgcagga tgaaaaccat tccatcctga tgtcctccca tatcaccacg gatttggaaa
    12841 aggttgccga ctatattact tttattcatc agggaaaagt cctgttttgc aagacaaagg
    12901 atgaactgcg ctacaaatat ggaatcatcc gctgcggtgc agccgttttc gatcagattg
    12961 ataaagcgga gatcctcgct taccgcaaag acgattacca gtggaatgtg ctggtggctg
    13021 ataaagaaaa ggccaggaaa aaatataaag cggccgtcgt tgatgatgct acgattgacg
    13081 atattctgct gctgtatgtg aaaggagagc gggcaaaatg aaaagcctta tattgaaaga
    13141 tttatataat atcggacaca atacaaaatc tatgctgttt atacttgcgg tgtttgctgt
    13201 ggcttttatt cctacctctg gtgtgggagc atatattttc atctgcggca tcttatgcag
    13261 tatgatgatc gtaaccactt tttcttttga cgataactcc aaatgggcgc gatatgcgat
    13321 gataatgccc atttcaaaaa gagatttagt gattggaaag tttattgtct tagcaatctt
    13381 ttgtgcgatt ggaagtgtat tcggcttggc tgtaggcttg atcggcggag gcatagcagg
    13441 caaaattaca ttcagccctg ccgctattgt ggaactgctg tttttaacct tagcggcaac
    13501 ggtaatttcc ctcatttttg gaagtatgtc aatctcgctg gtgtttaaat ttggagccga
    13561 aaaagggcgc gttttgcttt tggtatcgtt cctcattcca gcagcaattt gttttggggc
    13621 atataagctg cttgcaatac tgggcgtgga gctgacagat cagaaggtgt ttatcctttt
    13681 atgttgttcc ccgcttatcg cgttggtttg gagttatgga atgtatcaga tcagttatcg
    13741 gatttttttg aaacaagaat tgtaagatag gagagattgc tatgaaaaaa tatgagtatg
    13801 ttaatatcga atattcagca aaaggagtga tgttttctta tgttgaaaag catagggaaa
    13861 tcatagacag ttacgcggag aaaggctttc gatacgtcgg ttttgtccct acggaagttg
    13921 gggcaaacgg ctgcatcaga aagattgact tggtttttga aaagtgatta tgttttcaat
    13981 cgaaaatatg ggcctggcgt attatcgagc agtttgaggc gaaatgattc aaccataaat
    14041 ttaaaattca caaaaaatag gacaatagaa ggatacatcg ggaaagaaag cagaatctgc
    14101 cctgatacaa tttacacaac aaaagcagat ggaagaagtc cactgcttaa gtcaggtgaa
    14161 ttgaagggga ggaggtgcag tatccttata gagggaataa aaattctcct tacactgttg
    14221 tccgtggttg aacataagtc ttacaactga atattgtttg gcggcctgtt caaaagcgta
    14281 aacaacaaat gcaccgatgc ctgatattat atcgggtatc ggtgctttct tcattgctgt
    14341 tcaggatgaa aggcaagcca ttttgctcta taggtatcca aataaaaccg attcccatac
    14401 tcattttgga caaaatgctt tttgacctga taccagcccc gcaattcagt ccgcaaattt
    14461 gagtctggag agattggctc cagccatatc cgggtaaact gctggatctc agataagatt
    14521 agcggttttc cggatggcag actcggttcg gaaaataact ggggcagcgt aaacagcctt
    14581 ttggcatcag caaacggaac ggtctgtcca tttgggagca atagagtatg attgccggca
    14641 tcaaccagca tccatccata ctccgcagac cagactggtt ttccgtgaaa gattggaagg
    14701 gtttccggag caattggtac agattggacc gagatcccct gctctgaccg tccgaggaga
    14761 aaatccgttg ttacaccata gagaacagac attttctcaa gtccgtcaat ggaaggagac
    14821 ttgcgttctc cctcccatgc gcttagggtt ggctgagaga tgccgagttt ttcggcagcc
    14881 tctgtcatct ttaatttgtt gatcagccgt gcctgtttga attgtcttgg catctgttca
    14941 ccacctttat tcgtttattt gccaataacc atttttatct gagccgattc tttcaataat
    15001 acctttttcc tttaagtttt tgattctccc aaatacagct ttccttccga tattcaattt
    15061 atcagcaaga ttttgatagg tgtaagcagg atcctcagaa agaagagcaa gaatctcttt
    15121 ttctttgtca cttaaattta cagttccgtt tacagttcca tttacagttc catttgcagt
    15181 ttcccctggt ccatatacaa tccgatcttt tggtgcaata aatttgatca taaccgaatt
    15241 tccggtaata tcgtattccg gcaatggtac accatcttca cggcaggcat cacagatctt
    15301 ttctatgccg cgcccccagc tttcaataaa accggcaaga taatatacat gggcaatatt
    15361 agggttaaag ggctcagacg agtgcttttc catgagcttc tctgcagacc agttttcagg
    15421 cagtcggccg cagtttgcaa tatataatct gtcattatat acacttacct gaatcggtat
    15481 gccacgggca tagtccttat ggcacagggc attcaagaga gcctcgcgca gtgccgcatc
    15541 tgggacgaaa taacgctcga tccgctgcat tccttcgtat gtgatctttg ctttcatata
    15601 cttcagatgg atagtttcga taatccgatc aatctggtca agcagtgaac catgtatttc
    15661 atcctgatac cgaagttcag aatcactttc aaaataaccg atttttgtaa aagcaccaag
    15721 ctgccactct tcgggatcgt ttgcaaaaag gagcatggca gcatttgtta agtagtcatt
    15781 attcattaag tgcagctttt tcatcagcgt ctctttttgc tcatctaaag cgctctcatc
    15841 aatacgtccc tttttggccg cccactttat aaaacgctta acaacaccat tatcaatatc
    15901 agagattgtg aaggacggca aaggaaggtt gtcccaggtt gcgccctgtt tacggagcaa
    15961 aaagctctcc agttccaatc cggttaattt ctgattcgta ctgccactgc ggtaataata
    16021 aacgcctttg tatgagatag caataggata gggctgtaca gaaatttcaa tatactcggt
    16081 ttcgccatct gtatgaaggt tgacaggcac aataataccc atagcatcac ggattttatt
    16141 cggaagatcc tcaagcagct tatgggaatt agacaggcct acggtttcgc cattatcgtt
    16201 tttgccgatg taaagcgtgc cgccctgtgc attcgcaaaa ccacagatcc atgccagata
    16261 gtcatctttc caaatttctt tccattctat aatctgcatc tcaggcattg ctttgttcct
    16321 cgcatttctt tatatattta ccgcttttaa tgcgtgcatc ggctggcttt tctgcatctt
    16381 caggaataag ccatgtgtta ccggtcttct gagttccggg gatacgaccc tcgctgcaga
    16441 ggatacctac acggcgagaa gaaagtcccc actttttgcc ggcttccgtt gttgaaataa
    16501 atttcataat ttatcgcccc cttaacttta tatattatat tcggtttgcg gaataatatc
    16561 aagggttttg ctaaaaatgt cagaatgatt tccggctaca cgttttcctt ttcttatttc
    16621 ttacctcatt tttatacata aatcatatat ttatagctat tgactttccg cttgtaacaa
    16681 gttataatat gactataaaa tcaatttagt catataggag gcataaggta tgtatgaaga
    16741 agttggaatt tcttcaaata atgttccgat aacactttct atttggtata cagaaagaag
    16801 gagtcctacc attgtttttt tgccaggtac tatgtcacac cctctcatgt atgagaattt
    16861 tctatgtgga ttgagtgaaa gaggatttaa tataataggc gttcattatc tttcacacgg
    16921 aaaaagccct aaaatcaaga aaaattatac aatggaagat atgttgcata atgtatatga
    16981 cgcaacgact tacggaatag aaaatttcgg tgaaaacata gctgttatgg gttccagtca
    17041 aggcgggatt ttagctgcaa tggcggcagg taaagataca agattaaaag ccgtttttcc
    17101 acataatatc atgctgacta cgctaaaaga aactatgagc ctaacaaaat atcctgcatg
    17161 gacacaccgt tttatggggc tgatacaatg gggctttcgc gtgggcggaa agttgttacc
    17221 taactataaa gtaccgggag atgcttattt agactatgac agagtatttt atagtgaaca
    17281 ggcaaaacaa gattgcttaa acgaccctat gcttttacct tattatcctt taagatttgt
    17341 cgccagttta ttcaatgctg acttatcgtg cctggagaat gggtctatac aatgtcctgt
    17401 tattttaatt actgccaaag gcgatccgct ttttagcttt gattatacca gcaaagtatt
    17461 tgatttaatt catgcgccgc aaaaggaatt acttacttta gagcttaatc gccacatgat
    17521 ttttaatgag gatacagaca cgacgataaa cgcattggaa cctacattgc ataaatatct
    17581 cggatagtat attgatcata tgagcagtaa atgttatact tttcgtgacg atactaacaa
    17641 tttagtcata aggaggttat gagaattgcc acggtttaca gaacaagaga aagagatcat
    17701 cagcggtaaa cttttgatag agggagaaaa gctgtttgca ttacacggtt tgaaaaaagt
    17761 aacggttgat gatttagtgg ccgccgtaaa catatcaaag gggtctttct atgcttttta
    17821 tcccagtaaa gaacatctat atgtggaaat caattttcgc ttacaaaaag agctatttga
    17881 aagtatagaa acaacaatta aaaagaaaaa atataagaac catagagatt tagcaaagga
    17941 cgctattaag ctaagtttaa cgggtttgat tacttcacct attctttcgc agattgactt
    18001 atctataatg gactatctgc aaagaaaatt atcaccggat atttttgaaa gccatatgca
    18061 caatgatatt cgtatactgg agatactgga agatctaggg gttgtatttt tggttccgca
    18121 tacggttatt ataaaatccc tgtattctgt tttgtcctgt ttggaacaat tcaaagagga
    18181 tgaagaactg aatatgatac aaaacctttt agtgaatggg attattcagc aagtagtagc
    18241 agagtaacaa ttttgtttgg ttatttgaca ggcaggtagc caaacaaatt ttttacattt
    18301 tatatgacta tatatctata agcgtcataa gattaaagga ggaaaaataa tgattgatgt
    18361 aaaaaaactg tatttcagct atactgaaaa gccctttgtt gaagatgtga gtttccatgt
    18421 tggacgtgga gaaattttcg gctttttagg accctctgga gctgggaagt caaccataca
    18481 aaaagtattg actgggctta atacaaaata taaaggcagc gtaaaagttg caggtattga
    18541 aataagggaa cgtacaaatc ggttttatga gaatattgga gtggactttg aattttctac
    18601 ctgttatgag aaatttacgg cacgacaaaa ccttgcctat ttcgcttcgc tgtatgaaaa
    18661 gcagccccgg tctattgatg aattattgca tatggtaggc ttggaaaatg acggagacaa
    18721 aaaggttgcc gacttttcca aaggtatgcg ttctcgcttg aattttatca aagcgttaat
    18781 ccatgacccc gatatattat ttcttgatga accaacaagc ggccttgacc ctacaaataa
    18841 tcgcttgatg aaagatatta tccttgcaga gaagaagcgc ggaaaaacta tcataatcac
    18901 cactcacaat atgtatgacg caacggagct atgcgatcag gtagctttta ttgttgcggg
    18961 aaaagtcagc gcgttagaca gcccacataa tttaatcatg tcaaggggtg ctgcaaaaat
    19021 acaatatacc tattatgaca acggcgaaaa aaccggggaa tgtctgcttg atagaaccac
    19081 agaggataag atgttaaaaa atttaatttc tgaaaatcgt ctgctttcta tccatagtag
    19141 tgaac
//
LOCUS       DAZUMK010000328        14217 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.guided.066, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000328 DAZUMK010000000
VERSION     DAZUMK010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.guided.066"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..548
                     /locus_tag="ACG808_003064"
     CDS             <1..548
                     /locus_tag="ACG808_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFS5048582.1"
                     /translation="NSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAEKYKQEFTKRG
                     VTTNYFILPLRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQKK
                     LVTDNQAIAHYQFYHSENIFLLQERDLDELPEFLHTPFQPIPSELLEFFDAENWAKRF
                     FTTDSAIYEQYEYDLSSVKNK"
     gene            complement(691..1632)
                     /locus_tag="ACG808_003065"
     CDS             complement(691..1632)
                     /locus_tag="ACG808_003065"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFS5048583.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            1758..2879
                     /locus_tag="ACG808_003066"
     CDS             1758..2879
                     /locus_tag="ACG808_003066"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFS5048584.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            3137..4639
                     /gene="eat(A)"
                     /locus_tag="ACG808_003067"
     CDS             3137..4639
                     /gene="eat(A)"
                     /locus_tag="ACG808_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFS5048585.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            4706..5233
                     /locus_tag="ACG808_003068"
     CDS             4706..5233
                     /locus_tag="ACG808_003068"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS5048586.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQALAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELISYE"
     gene            5445..6506
                     /locus_tag="ACG808_003069"
     CDS             5445..6506
                     /locus_tag="ACG808_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFS5048587.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQNRLIELGSEAKKNKLELMVDISGEALKRAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPNDIDELKEAAADFSQIEAWHNYYPRLETGLDKEWYQEK
                     NQWLKTQGFLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSEHVQEQFASFQKEQTVLLHTEPVDEVFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPVRNTAKRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            6509..7405
                     /gene="murQ"
                     /locus_tag="ACG808_003070"
     CDS             6509..7405
                     /gene="murQ"
                     /locus_tag="ACG808_003070"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFS5048588.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELPSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISEHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEAAQAKLAEANGFVKGTL"
     gene            7419..9401
                     /locus_tag="ACG808_003071"
     CDS             7419..9401
                     /locus_tag="ACG808_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFS5048589.1"
                     /translation="MAKNELTLEELAKQIYQGAGGMGNVESIVHCMTRVRMKVINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQEMVDLAGVKLGEKLPSASATTAS
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAGTWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALATGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKAVPQASA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVISSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAADK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9471..9917)
                     /gene="nrdI"
                     /locus_tag="ACG808_003072"
     CDS             complement(9471..9917)
                     /gene="nrdI"
                     /locus_tag="ACG808_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFS5048590.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEYEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQRFGFPFLADYELRGTPSDVTRVYQILAEKNK"
     gene            10128..11552
                     /locus_tag="ACG808_003073"
     CDS             10128..11552
                     /locus_tag="ACG808_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFS5048591.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNVASIVMSTIFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFINNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGFLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(11729..12502)
                     /locus_tag="ACG808_003074"
     CDS             complement(11729..12502)
                     /locus_tag="ACG808_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5048592.1"
                     /translation="MTLLIKQELFKLIKKKSTAVLSVLLVALLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMESQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSIYLYLLAIIVTVLMKLILFPSISFTEKISTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIALFLALGYLVFKRKNV"
     gene            complement(12499..13404)
                     /locus_tag="ACG808_003075"
     CDS             complement(12499..13404)
                     /locus_tag="ACG808_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5048593.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSEGTIRIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVKQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDQAARTILTDAGYPLEDPDKIKLLKKNEELLGTVIQLLSAENVHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(13565..13885)
                     /locus_tag="ACG808_003076"
     CDS             complement(13565..13885)
                     /locus_tag="ACG808_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFS5048594.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGLLLISIIGIKI
                     TSGH"
BASE COUNT         4516 a         2556 c         3025 g         4120 t
ORIGIN      
        1 ataatagttc cttttattcg aaacatatgg atttttcttc tagcgaacca aagacggatc
       61 tattttttgg tgcaacaaat aacggcagaa aagaacgggc agaaaaatat aagcaggaat
      121 ttacaaaaag aggtgttacg accaattact ttattcttcc gcttcgagga aatgaacaac
      181 ctggatacct tacttatgaa gaatatctga aaaaaaccgg ggaatcgagg gggattctag
      241 aactgttgcg ggaagggcag caaggagtga ctttgcgaac gtttgaatct atgtattttc
      301 aaaagaaact agtgacagat aatcaagcca tagctcatta tcagttttat cattctgaaa
      361 atattttttt gcttcaagaa cgtgatttag atgaattacc ggaatttttg catacgccat
      421 ttcaaccaat cccatcagaa ctgttggaat tttttgatgc agaaaattgg gcaaaacgtt
      481 ttttcactac tgactccgct atttatgaac aatatgaata cgatctttcc tctgttaaaa
      541 ataaataaaa gtgccaacca atcatagatg aaaataaaca agcgcaattc ttatggcaac
      601 atgagaattg tgtttttctt tttgaacttt catgggggta aaaaaatgag aaccagctgc
      661 tgatccagct gattctcatc agagagcctt ttattctgcg ccaaggattc cttcttttgc
      721 atggactaat tgggtcaaaa aggcacaata tggtgtagca atatcatatt tttgtccttt
      781 tcgccaaacg gcaccattga tgtagtcgat ttcagtcaaa cgatggtttt tgatcaggtc
      841 ttggtacata gatgggtaat gcaacccgat gttgtcagga tcataagtag aagcaatatg
      901 ttggtacact tcttcttgat ctaatgtgat tccttctttt gctgcgatag aagcaaactc
      961 agatacaatc gttcgaacca tgccctctgc cgctttttgc gcacctaatt cagcaatatt
     1021 acaatccaat atcgtgcata atccatttaa tgttccgttt acacaagctt ttcgccaaat
     1081 ggaataacga acattatgac tatagattgg attcaatttt gcttcttgaa agacttctac
     1141 aacttttttt gtaaatgctt cgccttctgg ttccaagttc tctagttcga tttctccatc
     1201 acctaataat gtgacttttc cgggaccagc tagaccagct gtccacatcg tgatcccaaa
     1261 taagatattt ttctttggta catatttttc cagtacatct tcatgaccta atccattcag
     1321 taaacacaaa acataagtat tctcagtaat aatggattgg atcgaacgaa acatatcatc
     1381 taattgattc gcttttgtca aggctacgat caaatccacg tgtttgttgc tttcgatgat
     1441 ttcttctggt gaatagattg gcagttttgc aactacttct ttgccattaa agtcagcaat
     1501 caatccattt gtacgaatgg cttcaatgtg agcaggccag cgatcgatca agacgacctc
     1561 atttccgctt tgatgcaaca ttaatccgat tcggctgccc attgctccgg ctccagctat
     1621 tgcgattttc atgttttttc tctcctttat tcaatgagtt cccttttagt atatagaaaa
     1681 acagaagaaa atagcattgt tttgtttgaa aaaagtgaaa aaaagtgtct tacttaagaa
     1741 gaaagtgagg gatatagatg gatattttgg cggcaatcga ttcatttaaa ggaagtgctt
     1801 cttcatttga attaaatcag gcagttctaa atggttggga agaccgtaaa ggaagaaaaa
     1861 tcaatgtgcc gattgcagat ggcggagaaa atactgcaga agcaatctat tacgcgctcg
     1921 ggggaacctg gcgtacgatt gatgggatgg atctcttatt tcgtcagcga aaaatcagtt
     1981 atttattgac gaactggcaa ggaaaaaata ccgcagtcat tgaatctgca caagtattag
     2041 gattagattt acttgataaa ccaacggatc aaaccatccg acttgcttct agttatgcct
     2101 tgggagagct tatcaaagat acactgcttc aagaagtgga acaaatcatt gtcactttag
     2161 gaggaagcgg atgtagcgat ggtggtctag gattactcca atctttgggt gcttctttag
     2221 aagggtacac agaaggaaat cctttgttat ctactagaaa acttcatttg gatcgaagga
     2281 acgttcttgt ttctaaagta aaagtgatca tcgctgcaga cgtgaccaac ccatatacag
     2341 gtcaacaagg agctgcttgg caatttggaa gacaaaaagg aggaacggat acgacgttag
     2401 ctttcttgga taaacaagct gaaaagattg ctcagcagat aaaagatgtt tgggaggtgg
     2461 atttagatga catcccagga agcggagcag caggcggtat tggcggagcc ctttttttgt
     2521 taggagcgaa gatggtctct ggttttgagt tggtcagcca aatcactgga ttagaaaaac
     2581 agatcgaaca agcggatgtc atcattacag gagaagggcg gattgatcag caaacaatcc
     2641 acggaaaagt tccatatggc gtagcagtgc ttgcaaaaaa atatcgaaaa ccaataatcg
     2701 ctatttgcgg aagtagagac cgagagctgg aaggactctt tgattttctc cccattgtct
     2761 taagcattca attaggacca atcgatttag aggaagcaat ggaacacagg caaacattag
     2821 aaaaaacaga aatcttagga cagacactca gcagattttt caacattctc ccgaagtagt
     2881 tgacaaaaac aatttcttta tgctatcgta atttcaaata taggacaaag tgagggatcc
     2941 gtgcattgaa aaattaagtc gattgatgaa agctgcattc agactgtacc ttgtggagac
     3001 aagggagcag tgtgcagatt ttcgggattg gcttggttct ttcgatgtat gggtcttttt
     3061 gtgtgcccca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     3121 acgaggtgat ggaataatgt ctaaaatcga aataaaaaat ctgacattcg gctatgacag
     3181 ccaaggcaca ttattatttg aacaagccaa tctaaacttt gacacacaat ggaaactagg
     3241 acttatcgga cgaaacggtc gaggaaagac aactttactg aatatcctgc aaaacaaact
     3301 cccttatcaa gggcaagtga tccatcagca agcgtttgtc tatttcccgc agcaaacaaa
     3361 agataaagag cgtttgacct attatgtgtt aaatgagatt atggattttg agatatggga
     3421 aattgaaaga gagctccaac tgatgcaaac agatcctgaa atcttatgga gagaattcag
     3481 cacactatca ggaggagaga agacaaaagt cctactggca ctcttatttg tggatgacac
     3541 tcatttcccg ttaatcgatg aaccaacgaa tcatttggat atctctggca gaaaacaagt
     3601 agcggcttac ttgaaaaaga aaaaacaagg attcatcgtg gtgagtcatg atcggggatt
     3661 tatcgatgaa gtagtggacc atgttttagc aatcgaaaag agccaactgg aactttatca
     3721 agggaatttc tctatctatg aagaacagaa aaaacttcgt gatgaatttg aaatagctaa
     3781 aaatgaaaaa ttgaaaaaag aagtcagcag gctaaagaaa acagcagctg aaaaagcaga
     3841 atggtctcgt tcccgagaag gggacaaaac aaagaaaaaa gtcggattca tcgatacgga
     3901 atctaggcga gtgaataaag gagcagtagg cgctgatgct gcacggacga tgaaacgatc
     3961 taaagcaatc gtgaatcgga tggaaaccca gatcagtgag aaagaaaaat tattaaaaga
     4021 tatcgagtat atcgatgctt tgacgatgaa tagccaagtg tctcatcata aacggctgtt
     4081 aagcgtagaa aatcttcaat tagggtatga aaatctgtta ttcgagccag ttcattttac
     4141 gatcgaacct catcagcgga tggcaatttc aggtcctaac ggcgcgggaa agtcatccat
     4201 tatccattat cttctgggga cattcaacgg caaggttgta ggagaaaaat cccagcccaa
     4261 acatttgacc attagttacg tacgccaaaa ttatgaagac aatcgaggga cattggcgga
     4321 attcgcgaag aaaaaccgag tagactatca agcatttttg aataatctcc gaaagcttgg
     4381 gatggaaaga gatgtttttc ataataaaat cgaacagatg agtatgggac aacggaaaaa
     4441 agtggaattg gctaaatctt tatcccaacc agctgagctc tatatatggg atgaaccatt
     4501 gaattatttg gatgtcttca atcaagaaca attagaacaa ctgattttga acgtgaaacc
     4561 tgctatgtta ctagtggaac atgatcaaac ctttctagat aaagtatcta ctgagattat
     4621 ttctcttgag aaaatctaaa ccttgtaatc tcaaagacaa caaggtatgg ttaaggagaa
     4681 ctttgtcaac gaaggagaga atcacatgct ttatgtaacg agacacggag aaacaacttg
     4741 gaacgcacaa ggattagttt gcgggcgagc agatgtgccg ctgactgaaa aaggacagat
     4801 gcaggcacag gcactagcag aaaaggtagt agatttgcct gttccaatca cgaaaatcat
     4861 ccattcccct cttcaacgag caagagatac agcacaagca gtagttgatc gtctcaattt
     4921 gccgatgacc gctgatgagc gattggtaga aatggatttt ggggattatg acggactgcc
     4981 aagcaaagat gaaaactttc aaaaagcgcg tttagcgttt gctgtacgtt tcccaaatgg
     5041 agagtcggtc ttagatgtgt acgcacggat cgttccttta ttgaaagaat gcatggaaga
     5101 cgaagaaaat gtctacttgt tagtctgtca taatgccttg ataagagtga tcaatgctta
     5161 ttttcatcca atgcctaatg aagggttctt tacattcatg gtagacaata cagaattaat
     5221 ttcttatgag taattagact tttgtgttat ttaaataaaa tgtttcaagg cactgctttt
     5281 gcttagcagt gccttttttg tggctgtgtc aattcttttg gcattataaa tccaattttt
     5341 agaaataaaa tttaattatt ttaacaaaag tattgaaatt ttgtttcttt agatatatac
     5401 tctagtcata gaaatgaaga cggatacaga aaaagaggga taatatgttt ggcttttcaa
     5461 tatttatgaa tgattctttg tcagaagaga agaaacagta tatccgccaa atggcatcca
     5521 atggctttgt cggtattttc acttccatgc atataccgga agatgatgtt tcggcttatc
     5581 aaaatcgatt gatcgaacta ggaagcgaag cgaaaaagaa taaactagag ttgatggtcg
     5641 atatttcagg agaggcattg aaacgagctg gcttttcatt agaagatcta acacccttga
     5701 aagcaattgg tgtaacaggg ttaagaatgg attatcatat ttctaatcag cagatcgccg
     5761 actggtcaca tcaattgaaa atcagtttga atgccagcac gatcacgcct aatgatatcg
     5821 atgagttgaa agaagcagcg gctgatttct cacagattga agcttggcat aattattatc
     5881 cacgtcttga aacagggttg gacaaagaat ggtatcagga aaaaaatcaa tggctcaaaa
     5941 ctcaaggatt ccttgtacaa ggctttgtac caggagatac agagttgaga ggtccactgt
     6001 atcaaggatt accaactttg gaagaacatc gagggatcca tcctctggca ggtgctcttg
     6061 atctattagc aagtaacacg gatattgttt atatcggaga tgcaggtctg tcagaacacg
     6121 ttcaggagca atttgcttct ttccaaaaag aacagacagt cttgctccat acggaacctg
     6181 tagatgaagt tttttacgaa tatatattag gaaaacatac gaatcggcaa gatgatgcaa
     6241 gagatgtcat acgaagcgca gatgcgcgtt ttcgagagat tccgccaatc ccagtaagaa
     6301 atacagctaa gcgtatgaaa ggatctatta ctttagataa cgaaaagtat ttgcgctata
     6361 tgggtgagat ccaattaact aaatatgatt tgccagcgga tgaaaaagtg aatgtagtag
     6421 ccaaagtcat caaagaagaa ctaccgctga tcaaccaaat caaagctggt atgaactatc
     6481 aatttataag aaaagaagga agataaaaat gagcaaaatc aatttagaga acctaacaac
     6541 ggaacgcaga aaccaagaaa cttttggatt agatgaaatg agtatttctc aagcagtcca
     6601 actcatgaac aaagaagatc agaaagtggc cgctgctgtt gaaaaagaac tgccaagtat
     6661 cgagccagtg atcgccaaca cgattgaatc ctttaaaaaa ggtggtcgct tgatttatat
     6721 gggagctgga acgagtggta gacttggtgt attagatgct gctgaatgtg taccgacatt
     6781 cggtgtagaa ccggaaatgg tcataggcct aattgctggt ggagaagaag caatgactgt
     6841 agccgtcgaa ggagccgaag attcttatga attagggcaa gaagatctaa tcaagctgaa
     6901 tttaacaaaa aatgatatag ttattggtat cgcagctagc ggtcgtacac catatgtgat
     6961 tggtggatta gattacgcaa aatcgattgg tgctgttaca ggaacgattt cttgcaataa
     7021 acaagcagaa atcagcgagc atgcggattt cccaatcgaa gtcgatgcag gaccagaatt
     7081 tttgacaggc tctactcgat tgaagtcagg aacagcacaa aaactgattt tgaatatgat
     7141 ttctactatc agtatgatcg gtatcggtaa agtattcaat aacttgatgg tcgacgtcaa
     7201 accaacaaat gaaaaactaa ttgaacgctc aaaacgaatc atcatgcaag cgacagaagc
     7261 tgactacgaa acagctgaaa agtactttga ggaagcagat caaaatgtca aactggctat
     7321 cgtcatgatt ttgacaaata gtgataaaga ggcagctcaa gcaaaattag ctgaagcaaa
     7381 cggttttgta aaaggaacct tatgaggggg aaaagaccat ggcaaaaaac gaattgactt
     7441 tggaagaact ggcaaaacaa atttatcaag gagcaggcgg tatgggcaat gttgaaagca
     7501 ttgtccactg catgactcgt gtacgtatga aggtcatcaa tgatgatctt gtaaatgtct
     7561 cggaattaaa agcgattcca ggagtacttg gcgtagtaga ggatgaacag ctccaagtca
     7621 tcatcggacc tgggaaagtg aacaaagtag cgcaggaaat ggttgactta gcaggcgtga
     7681 aattaggaga aaaacttcct tctgctagtg caactacagc aagttcttct gcatctggaa
     7741 aagaccgtgt aaacgaacgg gcacaagaaa tgaaagcggc acaaaaatcg aaacaaaaac
     7801 cctctaaaat caaatcggta ttaaaagaca tctctaatat ctttgtacca atgatccctg
     7861 catttgtcgg cactgggatc gtggcaggga ttgccgcagt tttagcaaac ttagtcacag
     7921 ctggaacact agacgcagga acatggcaac aatatatcga tgtgatgaac atcttgaaaa
     7981 acgggatgtt tagctatcta gttatctata caggtatcaa tgctgcacaa gtttttggtg
     8041 caacgccaac gcttggtgga gttatcgggg ctgttgtgat gctgacaggg atgaatccag
     8101 aagcaccact gaaaaacttg tttaccggtg aagctcttgc aactggacaa ggcgggatct
     8161 taggtgttat cttcgcagtt tggttattgt caatcgttga gaaaaaactg cataaagtag
     8221 tacctgatgc ggtagatatc atcgttacgc caactctttc cctgattgcc atcggtctga
     8281 tcgaaatttt cttcatcatg cctttagcag ggttcatttc tgacggcatg gtcggtggga
     8341 tcaactgggt acttggtgtt ggaggagcat ttgccggctt cgttctaggt acattattct
     8401 tgccaatggt catgttcgga ctacatcaga tcttgacgcc aatccatgta caaatgattg
     8461 atgaaactgg tcgtacctta ttgcttccaa tccttgcgat ggccggagca ggacaagtgg
     8521 gtgcagcatt ggcattatgg atccgctgca aaaaagacaa agaattaaca gaaatgatca
     8581 aaggggcttt accagtcgga atcttaggaa tcggcgaacc attgatttac ggagtaacat
     8641 tacctctagg acgtccattt attactgctt gtatcggtgg aggtatcggt ggggcagtga
     8701 tcggaatgat cggaaatgtc ggagcaatcg ctattggacc ttcaggagcc gctttgattc
     8761 cattgatctc tgatggtaaa tggttaggct atatccttgg tttattggct gcttacgctg
     8821 gtggcttcgt tgctacattc ttcttcggta ttcctaaaga ccgtttagaa aaagaagaac
     8881 tagcgaccga aacagccaca gtaaatgaag tacctgaaaa agcagtacct caagcttcag
     8941 ctgaaagtgt gactatctta tcatcagttg ctgacggaac agttgtttct ttagataaag
     9001 caagtgaccc agtttttgca caaaaaatga tgggtgaagg ttactttgta gaaccagaaa
     9061 acggacatat ctattccccg gttactggag taataagttc tgttttccca acaaaacatg
     9121 ctatcggcat tacgacacca aatggcttag aaatcttgct tcacatgggc atcaacacag
     9181 ttgacttagg cggtacgcca ttcgatgtga aagtaacaga aggacagcaa gtgacacctg
     9241 atacgcttgt agcagaagct gacttagaag caatcaaagc agcagacaaa gaaacctcca
     9301 tgatcgtctt ggtaaccaat atggatcgtg taaaaaactt tgtcttggaa aaaacaggta
     9361 aagcgaaagc aaaagcacct gtgatggatg tcaaagctta ataagaatag aaacaataga
     9421 gaacataaaa agaagaagcc gaaatttatt tttcagcttc ttcttttgtg ttatttattt
     9481 ttttctgcca aaatttgata aacgcgtgtc acatcagatg gtgttccccg taattcataa
     9541 tctgccaaga aaggaaagcc gaatctttgt gcgtattgtt tggctgtcag acaatattga
     9601 ttgttgaagt ttttattacc gctaccgact acaccaaggc aaagtttatg attgtcttcg
     9661 tattccagat attcgcgcat cgtttcggtc aagatttctg tgtcaccgtt gtctaaccca
     9721 ttcccgccgt ctaaataagt aggaacgaaa gtaaaaaatg gttctgtttc tttcgtaaaa
     9781 tcactatttt catggatttc ttttaaagta acttcaggat tgacaggatc ttctgcatgc
     9841 attttttcag catattctgc tagatgtttg gcaaaagcac gcgtatttcc tgaaatcgat
     9901 atatataaaa tgttcatgga tcatccccca attctttccc tttagtatag catcttttta
     9961 agtttttttg gtggtgtaat ttggaaaggg actaggcgct ttgcttgtat aatgagaaaa
    10021 aagtcggtag aattatattt ctattaaagt ttttattaat taagtaaaaa taaaaaaggt
    10081 ttgttatact ttttgataaa agcggtttta ctttggagga tgcttacatg gatacagagc
    10141 aacagaaaaa aggaattggt ttgattccac ttgctgcctt agtgattggt tcagcgattg
    10201 gaggtggcgt atttggtatt atgacggaca tctccggatc ggctggtggc ccagctatct
    10261 tcagctgggt actcgtagga acagcaatgt tgatgctctc cttatcgatc aataatatca
    10321 ataaaaaacg tccagattta aaaggcggga tctttagtta cgcagaagaa ggttttggcc
    10381 gtttcgctgg ttttatcagc ggttggggtt actggctcac atgctggtta ggaaacgtgg
    10441 cgttcgcaac attgttgatg agtgcattag gttatttctt ccccgtattc gaaggtggtc
    10501 aaaatgtggc atcgatcgtg atgagtacga tcttcttatg gggatatgca tggctagtga
    10561 atcgtggagt tgaaaatgct agctttgtca atgctatcgt cacattttgt aaattagtgc
    10621 cattattttt atttattgta gtagctatca gcaccttttc gatccaacaa ttcattaata
    10681 acttttctag cgatatcata ttgaatgcga atgggcagcc gatcccgttt tctagtcaag
    10741 tattgagttg catcatggtg atgctctggg tattcgttgg tattgaagga gcttctgtcg
    10801 taggttcacg agcaaaaaaa caatcggatg ttgggaaagc aacgattcta ggaatattcg
    10861 gtttgatcat tatttacgtc ttggtttcca tgcttccata tggcacaatg acgatgaatc
    10921 agctgcaaac gatcaaagcg caacctgcta tgggatatgt atttgaaatg atggttggta
    10981 aatggggggc ggtcgtgatc aacggcggtt tgatcatttc tttagttggg gtttggctct
    11041 catggacaat cttgccgatt gaaacaatgg ggaatatggc tgaggacgga cttttgccta
    11101 aagcatgggg aaaactcaac aaaaatgggg caccaactta tgctatcgta ttgacgacgt
    11161 tatgtacgaa tgtcttttta ctttcattat tattcactga cagtgcatat aactttgctt
    11221 atacacttgg tacagcagcg attttcttca cgtggctttt cttagggctg taccaaatga
    11281 agctttctta tcagcggaaa gagtgggtcc aatttttagt agggttttta gcaagtgctt
    11341 ttcaagtatg ggccatgcta gttgtagcct ttaatgaagt gatgcttgtt cttgttcttt
    11401 tcttaccagg tatttatttt tacctcaaag cacgtaaaga acaaaacgaa cctgtacaac
    11461 atatccactt agataagatt ttacttatcg gtgtgacgat ggtcggagta atcgcaatgg
    11521 ttttatttgc cactgggatg cttcacgtat agaagtataa agagaggctg tgaagcctgt
    11581 ggtttattca aaaaagaagc ggtgacaaca ttcgtgtcat cgcttctttt tttgaataaa
    11641 tggtgttcaa gaagtagctt cttctaaaaa taagccgaaa tttcttgaaa ctggccactt
    11701 ctgtcacaac ttctttgtta atgaaccatc atacattttt tcgcttgaat acgaggtatc
    11761 ccaatgctaa gaacaaagca atgtaagcta aattgccaaa cagcatttga ttagtagaaa
    11821 gatgagtcaa ttgcttcata atctcttcgt tgccaatttg gttttgcaga ttcagcatac
    11881 tgattggatt ccatttgatc cactcccatt tttgaatgag tgcgatgagg ataccagata
    11941 tgattgatga agcaaaataa aagacgatcc ctgctgagat ggaggcaccg gaactattga
    12001 taaaacaagc aatcatcaac actaaactta aaattagcca taaaccgata taactaccca
    12061 aagtataagt gaataagctt tggatcagtg tctgaccagt agaaatcttc tccgtaaagg
    12121 aaatcgaagg gaaaaggatc agtttcatca gcacggtgac aataatagct aaaaggtata
    12181 aataaataga ataaataact aatgtgatcc atttacttac taatatttct cctcgagaat
    12241 actttcgata aaggagattt ttcaatgtac catattgtga ttccattgaa atgatcgtac
    12301 ttgcagcagc aatcatgata aagacaatcc aagatgtggc actgaaaagc tgagcagtca
    12361 tttcaactgg atcaatgatc gtcgtgtatt ttttggctag aagtgctgtt cctatgagca
    12421 aagcaactaa tagaacagat aaaacagcag ttgacttttt cttgatcaat ttaaataatt
    12481 cttgttttat aagcaacgtc atttttcttc ttcccccttt actgaatcca gaatttccaa
    12541 tagggaagat tccaaatcat tcgtcagatg ctggacatct gtcacatgga cattttctgc
    12601 agacaataac tggataacag ttcctaataa ctcttcgttt ttttttagaa gcttgatttt
    12661 atcaggatct tccaagggat atccagcgtc tgtcaagatc gttcgagcag cttgatcatt
    12721 tgtcgtagag ataacgatga aatcttgcgc tgtttggttc aattcagcca ttgtacattg
    12781 tttgatgatt tttccttgat tgatcaggat cagctcatcg accatttttt caagctcgct
    12841 gagtaagtga ctggagatca agaaagaggt gccctgagta gccaagtcgg taatgatttt
    12901 tcgcacatct cttgttgctt gtggatccaa accgttcatc ggctcatcta aaataacgat
    12961 agacggatgg ttcagtaagg caagagcaat tcccaatttt tgtttcattc ccagcgagta
    13021 ggatttcgct tttttgtgaa tgtatttttc catcttgagc tgtttcacga tagtgtcgat
    13081 agcagataca tcgtttgttt cagagaataa cttcagatga tcataacctg ttaagaatgg
    13141 atagataccc ggatattcga tcaatgctcc tacttgttct aatcctttat gactagaggg
    13201 agaaatctca ttttcaccaa tacggatcgt tccttctgag taatggatca gtcctaaaat
    13261 agctttcatg atcgttgttt ttccagcgcc attaggacct actaatccaa cgattttgcc
    13321 aggagataag ctaaaactga catcgctgag ggcttgtttg tgaccaaagt tcttggataa
    13381 ttcttggacc gaaagacttt tcaaaaatat tcactccttt tctatagata gtacaagcat
    13441 acacctgatg agtagagaag tcgctaggtc attagtctta ttttcttgtt ttttcataga
    13501 aagaggaata tcaaataaaa agccccgctt tttctgatca gtccttgtct gcttgtctaa
    13561 gtatctagtg accgctagtg attttgatgc caatgatgct gatgagcagc aacccgacaa
    13621 agaaccaagt ggaaagattc agcttatcat gaaacaagac cacaccaata atgaccgaac
    13681 caactgcgcc aatccctgtc caaataggat aagagagact cataggcaaa cttttgattg
    13741 cctttgacaa aaagtaaaaa ctagcaatca ttccaatcac tgtgtaaaaa gaataattga
    13801 ttttagaaaa accatcactc catttcatca tcgttgacca gaatacttcc aaaattcctg
    13861 ctatgattaa ttccatccat gccatacata cgttcctcct tcaatataaa aaatacacaa
    13921 aaagaggaga atcatccctt caaagaccta atggaatgat catcctcttt tttactaccc
    13981 ggtggcagat cgtatatctt tttctgtagt atagcacacc tatccagata tcggcaatct
    14041 catacttttt agtaaagtgt aacaagcatt gacggatata tcgttagcag aaaagaagat
    14101 gaatgctttg caatttcata gaaaggagtt gttatttatt aatcatttaa aaaaacagta
    14161 actagacctt atttttgtat attagcaggc aataaattaa aaaaagcttt atcttac
//
LOCUS       DAZUMK010000329         6236 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.guided.134, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000329 DAZUMK010000000
VERSION     DAZUMK010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.guided.134"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..209
                     /locus_tag="ACG808_003077"
                     /pseudo
     CDS             1..209
                     /locus_tag="ACG808_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            447..683
                     /locus_tag="ACG808_003078"
     CDS             447..683
                     /locus_tag="ACG808_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048595.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            690..2642
                     /locus_tag="ACG808_003079"
     CDS             690..2642
                     /locus_tag="ACG808_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFS5048596.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2714..3211)
                     /gene="dfrG"
                     /locus_tag="ACG808_003080"
     CDS             complement(2714..3211)
                     /gene="dfrG"
                     /locus_tag="ACG808_003080"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFS5048597.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3486..4613
                     /locus_tag="ACG808_003081"
                     /pseudo
     CDS             3486..4613
                     /locus_tag="ACG808_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein"
     gene            4711..6084
                     /gene="tet(L)"
                     /locus_tag="ACG808_003082"
     CDS             4711..6084
                     /gene="tet(L)"
                     /locus_tag="ACG808_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HFS5048598.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
BASE COUNT         2070 a          983 c         1154 g         2029 t
ORIGIN      
        1 tggaactcga acaagaggaa agcataagat ttcagaattg ttctctgttc cctctttatc
       61 atggaagtgc aaaaagtaat atagggattg ataaccttat agaagttatt actaataaat
      121 tttattcatc aacacatcga ggtccgtctg aactttgcgg aaatgttttc aaaattgaat
      181 atacaaaaaa aagacaacgt cttgcatatt aagtagttca gttttggagt acaaaaagtg
      241 aaaatgtaaa cctaaatgag tatcaaattg caactgaatt tgatactcgt tttttattgt
      301 taaatcaacg tttgttgtta atttacacca caaaatgtaa gttaaaatgt atataaaaat
      361 gcgaacgtga aataattatt gtaggaatgc aaattggtgt gttaaaatgg ttacaattta
      421 ctaagcaaaa aggggtgcat tttcctatgc aatttcttga gccaaaaaat aagaacgcta
      481 agtctgtgga ttgggaaata tcagagcaag taagggtgat agttaagcaa tacgctgaat
      541 atgcagagcg tactgaaagc gaagcagtgg atgaattctt attgaacatt ttagacgata
      601 agaagtttat tgaatggatt gccaacaagc gaagtaataa gcggattgtt gaaaaaatgg
      661 gtatcaaaga tagagtgggt tgatagacaa tgaccaagct taaaagatta gccacgaagg
      721 aagatgaaat tgtagatgtt aaaattccga tttcaaacga agaattaaaa gatagggcta
      781 aacaatatga tttattaact ccaaaacgat ttgcaacaag gtataacaaa atgttgttct
      841 tgccaacgtc ttttaaatgg aacggttctg aatatcccat tcaatataat tactgcatca
      901 atccgttttg ttgtaatttt gggaaagaac agcacaaatt caaagatgta aaagggaaac
      961 caagtcgata taaaatgact ggtagttcca aagataaagg tcataaaggt atgtactgca
     1021 atgacaatcc tattggcaga ggtgtatcgc aaaactgtac agtaacccct ttatctaatt
     1081 ggtctgttgt tgaagaaatt aaacgcctta tagaaatcaa tagcatccaa gatgttgagc
     1141 cagattatca atttcataaa gaaggctgct cggaagaaga atcaacccca ttcaatgagc
     1201 cgaagcaatt ttacaaaagg gggaaaagta ggggcaaatc tcaacgctat caatgtaagg
     1261 cgtgtaagaa gttcactaat gttctaccaa aaagagaaga aaccactact tatcaccaac
     1321 aaaagaacac cattcttcca atgttcgcta agatggttgt tggaagggtt tcagtcagtc
     1381 gtacttgtga tattttagga attggagttg gaacctacta tcataagctg gagtggctgt
     1441 ataggcgttg tttggagttt ttagaacgat atgagacaca accccttcaa acaaagaaat
     1501 ttaacgagat gtggcttaat acggacaaga tgcactacta cctaaataac gtccgtaaga
     1561 aaggacaagg ttcaaagaaa tatacggggt ttgaagacct taatatgcaa acgtatatag
     1621 ttgtatctgc tgaggtctta tcaagatatg tgttccgttc tgatgttgcc tatgattgga
     1681 atatttcaat ggatgaatta aatgaagata caagaaaatt caaagaagac catctcaata
     1741 cttttagtcg taaaaatgat aggttagatt ggtcttatta tccacaagaa ccatccgcta
     1801 acgatagcga aaataggaat gcttatcttc acgaattagg gaaaattact aataggagta
     1861 gatttgtcga tggtttaaat gttgatgctc cttatactac aactgctcac tattggttaa
     1921 ttaaacaaat ggttaatgcc gacgaatggc gaatgattag tgatgatgac ttctctatta
     1981 gaaacgcctt ttatagagta ttcacgaaag aattaaggtt gagcgatgca catcatttta
     2041 tttgccaagt taacaagacg aaaagcagaa aacaatgctt aaaagaattc ggacaagcaa
     2101 aagcagaact gctcgattgg ggagatatca gaggattcaa aacaaagttt ctacggactt
     2161 tggcatctca ttatctaact gaattactaa caagtcatca attccacgaa gaggctatta
     2221 gtaaagatgg agaaagatat cgcaaatatg ccgataaccc tatcaaacat ccattagcca
     2281 ctaaggataa aggattttat tcagttgatt gtagaacgga tttatctgct ctggaaccaa
     2341 acgaaatcgc aaagatgcta ttaaatgtta atgaccattc gaccaatagc tttattcaac
     2401 agataagacg ttatatatca tctcttgaaa gaccattaac aacagctcgt ggtgataaga
     2461 agagctatat ttacgctaac ttcaatccga agtatgctca atttgcgatt acaattctaa
     2521 gaacttacta caacttttgt cgccctttta aatcagcgga taaaaaggtt ttgacacctg
     2581 ctcaaagatt agggattaca gataaacaat ttgattggaa agatataatt tatttcaaat
     2641 gacaaaaaga gcttggagta atcccaagct cttgtgcttt atctttactg ttcttcttca
     2701 attaacgcac ccgttaactc aataagtttt ttctttcata tacatgaaaa tagtagttat
     2761 acggattttt atcattcttt atcccttttt gggcaaatac ctcattccat tcctcataat
     2821 tcacttctgg aaaaaaagta tctccttcga attcatgatg tatttttgtg atgtacattt
     2881 tctcaacata agggaaaaac aaattataaa tctgttctcc tccgaaaata aaaatttctt
     2941 cttcgttttt acataactca aaaacatctt ctattgaatg aacaatttca caaccattaa
     3001 aggtaaaccc cttatctctc gtcagaataa tatttcttct gtcaggtaag gctcttccga
     3061 ttgattcaag gttcttccta cctaatatta tcggatgtcc ctttgtagta tttttaacat
     3121 attcccagtc cttgggaatc ctccaaggaa tgtcattctc tttgccaatc actctattct
     3181 tatccatcgc agcaatcaaa gaaactttca tttataacac agccttttta actaatttac
     3241 catctaattt cttatttcta tcaaaaaacc ttttctcatt atcctgcccg ttagttgaat
     3301 aagcaatttt ttctataagt tatattatca taataccaca aaatcacaaa ttttcattca
     3361 attatcattt tcaattgcat tttataaatc attttgtatg taatttggta tgtaagttaa
     3421 aatgaaaaaa gtagtccatt ttgaagtaca ttttgtcatt ttgtactcca aaactgaact
     3481 acttacttgc atatatacgc ctttatagtg gagtactaca tttacgagat tcggttagag
     3541 tatcagaaaa agaaaaaata aaagttacag aaatgtatac ttcaataaat ggtgaattat
     3601 gtaagattga tagagcttat tctggagaaa ttgttatttt gcaaaatgag tttttgaagt
     3661 taaatagtgt tcttggagat acaaaactat tgccacagag aaaaaagatt gaaaatccgc
     3721 accctctact acaaacaact gttgaaccga gtaaacctga acagagagaa atgttgcttg
     3781 atgccctttt ggaaatctca gatagtgatc cgcttctacg atattacgtg gattctacga
     3841 cacatgaaat tatactttct ttcttaggga aagtacaaat ggaagtgatt agtgcactgt
     3901 tgcaagaaaa gtatcatgtg gagatagaac taaaagagcc tacagtcatt tatatggaga
     3961 gaccgttaaa aaatgcagaa tataccattc acatcgaagt gccgccaaat cctttctggg
     4021 cttccattgg tttatctgta tcaccgcttc cgttgggaag tggaatgcag tatgagagct
     4081 cggtttctct tggatactta aatcaatcat ttcaaaatgc agttatggaa gggatacgct
     4141 atggttgcga acaaggatta tatggttgga atgtgacgga ttgtaaaatc tgttttaagt
     4201 atggcttata ctatagccct gttagtaccc cagcagattt tcggatgctt gctcctattg
     4261 tattggaaca agtcttaaaa aaagctggaa cagaattgtt agagccatat cttagtttta
     4321 aaatttatgc gccacaggaa tatctttcac gagcatacaa cgatgctcct aaatattgtg
     4381 cgaacatcgt agacactcaa ttgaaaaata atgaggtcat tcttagtgga gaaatccctg
     4441 ctcggtgtat tcaagaatat cgtagtgatt taactttctt tacaaatgga cgtagtgttt
     4501 gtttaacaga gttaaaaggg taccatgtta ctaccggtga acctgtttgc cagccccgtc
     4561 gtccaaatag tcggatagat aaagtacgat atatgttcaa taaaataact tagtgtattt
     4621 tatgttgtta tataaatatg gtttcttgtt aaataagatg aaatattttt taataaagat
     4681 ttgaattaaa gtgtaaagga ggaattaatt atgaagtgta atgaatgtaa cagggttaaa
     4741 tacaaccaaa ttttaatttg gctttgcgtt ttatcttttt ttagcgtatt aaatgaaatg
     4801 gttttgaacg tctcattacc tgatattgca aatgatttta ataaaccacc tgcgagtaca
     4861 aactgggtga acacagcctt tatgttaacc ttttccattg gaacagctgt atatggaaag
     4921 ctatctgatc aattaggcat caaaaggtta ctcctatttg gaattataat aaattgtttc
     4981 gggtcggtaa ttgggtttgt tggccattct ttcttttcct tacttattat ggctcgtttt
     5041 attcaagggg ctggtgcagc tgcatttcca gcactcgtaa tggttgtagt tgcgcgctat
     5101 attccaaagg aaaatagggg taaagcattt ggtcttattg gatcgatagt agccatggga
     5161 gaaggagtcg gtccagcgat tggtggaatg atagcccatt atattcattg gtcctatctt
     5221 ctactcattc ctatgataac aattatcact gttccgtttc ttatgaaatt attaaagaaa
     5281 gaagtaagga taaaaggtca ttttgatatc aaaggaatta tactaatgtc tgtaggcatt
     5341 gtatttttta tgttgtttac aacatcatat agcatttctt ttcttatcgt tagcgtgctg
     5401 tcattcctga tatttgtaaa acatatcagg aaagtaacag atccttttgt tgatcccgga
     5461 ttagggaaaa atataccttt tatgattgga gttctttgtg ggggaattat atttggaaca
     5521 gtagcagggt ttgtctctat ggttccttat atgatgaaag atgttcacca gctaagtact
     5581 gccgaaatcg gaagtgtaat tattttccct ggaacaatga gtgtcattat tttcggctac
     5641 attggtggga tacttgttga tagaagaggt cctttatacg tgttaaacat cggagttaca
     5701 tttctttctg ttagcttttt aactgcttcc tttcttttag aaacaacatc atggttcatg
     5761 acaattataa tcgtatttgt tttaggtggg ctttcgttca ccaaaacagt tatatcaaca
     5821 attgtttcaa gtagcttgaa acagcaggaa gctggtgctg gaatgagttt gcttaacttt
     5881 accagctttt tatcagaggg aacaggtatt gcaattgtag gtggtttatt atccataccc
     5941 ttacttgatc aaaggttgtt acctatggaa gttgatcagt caacttatct gtatagtaat
     6001 ttgttattac ttttttcagg aatcattgtc attagttggc tggttacctt gaatgtatat
     6061 aaacattctc aaagggattt ctaaatcgtt aagggatcaa ctttgggaga gagttcaaaa
     6121 ttgatccttt ttttataaca ggaattcaaa tctttttgtt ccattaaagg gcgcgattgc
     6181 tgaataaaag atacgagaga cctctcttgt atctttttta ttttgagtgg ttttgt
//
LOCUS       DAZUMK010000330         5533 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.guided.152, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000330 DAZUMK010000000
VERSION     DAZUMK010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.guided.152"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            52..516
                     /locus_tag="ACG808_003083"
     CDS             52..516
                     /locus_tag="ACG808_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS5048599.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            500..1129
                     /locus_tag="ACG808_003084"
     CDS             500..1129
                     /locus_tag="ACG808_003084"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFS5048600.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            1483..2961
                     /gene="msr(C)"
                     /locus_tag="ACG808_003085"
     CDS             1483..2961
                     /gene="msr(C)"
                     /locus_tag="ACG808_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFS5048601.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            3008..3454
                     /locus_tag="ACG808_003086"
     CDS             3008..3454
                     /locus_tag="ACG808_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFS5048602.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(3653..4168)
                     /locus_tag="ACG808_003087"
     CDS             complement(3653..4168)
                     /locus_tag="ACG808_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS5048603.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            4285..5487
                     /locus_tag="ACG808_003088"
     CDS             4285..5487
                     /locus_tag="ACG808_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5048604.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1731 a          924 c         1191 g         1687 t
ORIGIN      
        1 attattgaca agtcaatcgt tgacgagtca attattagaa aggaggaaat tatgtcttta
       61 gaagagatta gtcacctttt atatcaaatc aaattagccg atcaaaaaat cgtccagatc
      121 tttgaagaaa atatcggtat aagtcttact agatacgaga tcatgatgag actgaaagaa
      181 gaaaatccat tgctacaaag cagtttgcaa gaaaagttgc agattgatca aggagcagtt
      241 actcgtcatc tgaaggtatt ggaagcaaat ggctatgttt cgcgtcagag aaatcctcag
      301 aacaaccggg aagtgtatgt agcgctaaca gaaaaagccc agaagaaaat caagcattgt
      361 gaaatggacc ataaagatac aggttccgtg ctatccccgg ctttcagcga tgtagaaatg
      421 aaacaatttc taacactttt agataaattg aatacccaac tttgcaatat aacaacagaa
      481 acgaaagagg ttaataataa tggaacaaaa aaatgatttt tcaacaatta tgactggtag
      541 aagatccgtt cgcgtatatg atgaaacata caaaatccca cacgaagaaa tgctggaaat
      601 gatcaaagaa gcgacgactg caccttcttc tgtcaatatg aaaccatggc gctttgtggt
      661 cgctgaaagc gaggaagcaa aagaaatctt gcgtcctttg atccgtttca atacgagaca
      721 aaatgatact tcttctgcaa tggtcatgat ttttggcgat atgcaatgtt acgaatatgg
      781 cgaagaaatc tacaaccaag cttacgaatc agggaagatg ccaaaagaag tcagagatca
      841 acaattagct gcgatcattc cttattacaa gagtttttca agagaagaaa tgaacgatgt
      901 agtcaaagtc gattctagct tagcggctat gcaattcatg ttagttgcgc gtgatcatgg
      961 ctatgaaaca aatccaattg gtggattcga agcagatcag ttggcagagg catttggttt
     1021 ggataaagac cgttatgttc cagttattat tttatcaata ggaaaagcgg tagaagaagg
     1081 ttacgagtct gttcgtttag caccagaaaa gatcactatg ttcgaataaa aggttttcag
     1141 ttactattca gtagctattg ctttttttga atcgtgtgtg gtaacctaat aaaaaaggag
     1201 gtcggaaaaa tgactgcatc gatgaaatta cgtttcgaac ttttgaataa caactaaata
     1261 aattcaaaag acatgtataa acaacaattt catggatgga gtatgtccat tttttcgaac
     1321 gacagtagaa taggtgaaag ctatgacaga tttggtgatt tagtcatagt tatttcgttc
     1381 agagtaggaa aagatgtgat gcagtttttt tgcaaacaga tttttctgct cttttttagt
     1441 atcgataatt gttgttttac atgaaatagg agtgtaaaac atatggaaaa tttagcagta
     1501 aatataacaa atctacaagt cagctttggc aaccagctag aactatctat tgattctctt
     1561 cgtgtctatc agcaagatcg tatagggatc ataggagaaa atggtgtcgg taaatcgact
     1621 ttgctcaaac taatagccgg tgaacttttt cccgatcatg ggaaaatcca aacagagatc
     1681 accttcaact acctgcctca attaacctat ctcgctgaag caaaggacct aaatttggaa
     1741 ttagccagtc atttccagtt aagactggaa gaaacttcag agcggaaatg gagcggaggg
     1801 gaagaacgaa agatcgagtt gatacgtctt ctttcttctt atgaacaagg gatgcttcta
     1861 gacgagccaa caacccatct agatagaaaa agtattgatc gactgattga agagcttcgt
     1921 tattattatg gtacgctggt ttttgttagt catgatcgct attttctaga tgagctagca
     1981 tcgaaaatct gggaagtaaa agacggagaa atccgagagt tttcggggaa ttatagtgcc
     2041 tatctcactc aaaaggaatt ggagaaaaag actcagctac gagaagcaga gtcgatcatg
     2101 aaagagaaaa aacgattgga aaaatcgatc caagaaaaga aaaaacaagc ggaaaagttg
     2161 gaaaaagtgt ccagtaaaaa gaaaaagcaa caaatcagac tggatcggtt gtcttcctct
     2221 aaacaaaaag acagtgtaca aaaagccatc caaaagaatg cgaaaacatt agagagaaga
     2281 ctccaaaaaa taggagaaac aaccaagccg cagcagatga aacaaatccg ttttccagta
     2341 ccaaaatctc ttgaactcca cagccgctat cccatcatgg gacaaaatgt ccaattggaa
     2401 agaagtggga gaacattact ggtaaatggt gattttcagt tttctttagg taaaaaaatc
     2461 gcgattgtcg gtgaaaatgg ttcaggtaag acaaccttat tagaacatat ccgcaaacaa
     2521 ggagaaggaa tccttctctc tccgaaagta agctttcaag tatatcagca aaagggttat
     2581 caaatgacat ctgaagaatc catcattcgt tttgtcatga gacaaacaga gttttcagaa
     2641 tcgcttgtcc gtagtttgct gaatcactta gggtttgctc aggaaacttt gacgaaaccg
     2701 ttatgtacat taagtggggg agaagcgacc cgtctgacga ttgctttgct ttttactaag
     2761 ccaagtaatg tgttgctgtt agatgaaccg actaatttta ttgatatggc aacgatcgaa
     2821 gctttagaga agctaatgca aatatatccg ggaacgattt tgtttacttc tcatgattct
     2881 tactttgtcg agcgtacggc tgatgaagtt tatgaaataa aagggcagaa aataaaaaaa
     2941 gtacttacga gaaattttta aatgtgctac gatgattttc gtgacaaatt taaaggagtg
     3001 gtacagattg cctacaaata aaaaagaagg aataattttt acaacaatga tgtgtttttt
     3061 gatggtcttg gggatgagcg cgtataattt atggctgcat caagatcttc atttatcaag
     3121 tttgttgatt ggtctagtgc ctggattcgt ggtggctttc attttggatg tcttcatcat
     3181 cggtgtgctg gcgaaaaaaa ttgcctttaa actaccagta aatccagaaa gcaaggtcca
     3241 aatgatctta actatttcat gcttgatggt tttaggaatg gtgacatgta tgtctttatt
     3301 cggtgtgatc atggaacaag gaatacctga tcaattattc tctgcttata tgaatgcttg
     3361 gaaaatgaat gtgattgccg ctttaccgtt gcagttgatc atcgttggcc cgctttcaag
     3421 aagtgtgcta aaagcaattc aaagtaatga atgatggaac actgttattt gaaaaaaggc
     3481 cttggcatgc tttttgtcat agtctcttga atgcagttct agaaaaatag tgatccgaac
     3541 gataggtttt ggcctgtcgt tcggatcgct ttttcttttc caactgtatt gctcttttat
     3601 tgaagataat ttgctcttca atagatattt ctagaaaggt tctgtttatc ccttactcaa
     3661 aaaactgctg catttgttct cttacttgct gacaccattg gagttcctgc tgacaaagca
     3721 taattctgcg ctggatcaca acataacgtc caaattcttg tttcttgtct agcgagaggt
     3781 cattcaattt tttttgattt ttttgcatga ttttgttcag ctgttgttct ctagtgaata
     3841 gcagtccagt agctgttgtt ttatcgagcg tcgaaaaagc atagattttt gctagaaatt
     3901 catctttttg cgtaggagcg ggagtttcct ctttgatcca ttcctctaca agagcacgtc
     3961 cttcatcagt caagtggtag atcttttttt gagagtgctc gtctttgtcc aaaacggtca
     4021 cataaccctg ctggtctaac ttggcaagcg ttgtatagat ctgactgtga tgggcttccc
     4081 aaaaaattgt gatcagctgc ttcaattcat acccagaacg tggtttggat aaaagcacgc
     4141 ataataatat ataggctaaa gtattcataa aaatcctacc tctcgtctta ttttctttat
     4201 tatatcacag taaagtttcc acgttttaca tgtgcatatt gacatgtaaa ataaatcatg
     4261 tatgatgcta atagagagaa agggatggaa atattggaga atcaaataga tatacaagaa
     4321 caaacaaatg aagaaacaaa ggggaaaagt tcagcgctag ttgtagcagc tacctgtatg
     4381 atcgctttta tgggagtggg tctagtagac ccaatcttaa aaacgattgc tgcacagtta
     4441 aacgctacgc cagctgaaac gaccctgtta tttactagtt acatgttagt cactggtgtg
     4501 gtgatgctat ttacaggttt cttatctagt cggatcggtt tgaaaaaaac attgatgaca
     4561 ggattgttta tcatcgttgt attcgctggt cttggcggat tatctgggaa tatcggaatg
     4621 ctgattgggt tacgtgccgg ctggggtgtc gggaatgctt tatttgtctc tactgcttta
     4681 gcaacgatcg tcagtatttt agttggtaac acagaaaaag caatcatgat gtatgaagca
     4741 gcacttggct gcggaatggc tgttggccct ctagtcggtg gggtcttggg cagtggttca
     4801 tggcgttatc cattttttgg ggtagcattt ttgatgtttt gcggttttat agccctagcg
     4861 gtacttttac ctgaaactga gaaaccaaag agaaaagtag gcctttggga aggtgtcaaa
     4921 gcgttaagca atcataaact tcgttcagtt ggactaatcg cattgttata taatttcgga
     4981 ttctttactt tacttgcgta tgcgccattt ttattgattg gctattctga attagaagtc
     5041 ggttttgtat tttttggctg gggtgtgctt ctggctattg cttcgatttt cgttgcaccg
     5101 gctcttgagc ggaagtttac gacttatcgg acaattttag cagcatttgt cttattcatt
     5161 atttgtttgc tgttattagg agctggaact tctatgccag tccttgtacc gatcagcatt
     5221 gtgttagcag gatttttcca aggaattatc aatacactgt tgacgacgat tgctatggaa
     5281 ataccaggtc tgcaacgaaa cgttgcttcg tccagctata gttttgtccg ctttttcggt
     5341 ggagctttag caccatttat tgcaggtgtg attggtgaac atattggtga aagctatgcg
     5401 ttttattttg caggtgccat tgttcttatc agtgtatgtt gtatcattta tcaccgtaat
     5461 tattttgcta caaatgaaga agaataagaa atcagaggtt gtgaaaaagc tgtcctgcat
     5521 caagtcataa gaa
//
LOCUS       DAZUMK010000331         5206 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.guided.158, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000331 DAZUMK010000000
VERSION     DAZUMK010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.guided.158"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..209
                     /locus_tag="ACG808_003089"
                     /pseudo
     CDS             1..209
                     /locus_tag="ACG808_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            447..683
                     /locus_tag="ACG808_003090"
     CDS             447..683
                     /locus_tag="ACG808_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048605.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            690..2642
                     /locus_tag="ACG808_003091"
     CDS             690..2642
                     /locus_tag="ACG808_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFS5048606.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2714..3211)
                     /gene="dfrG"
                     /locus_tag="ACG808_003092"
     CDS             complement(2714..3211)
                     /gene="dfrG"
                     /locus_tag="ACG808_003092"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFS5048607.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3486..4613
                     /locus_tag="ACG808_003093"
                     /pseudo
     CDS             3486..4613
                     /locus_tag="ACG808_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
     gene            4711..4899
                     /locus_tag="ACG808_003094"
     CDS             4711..4899
                     /locus_tag="ACG808_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFS5048608.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(4957..>5206)
                     /locus_tag="ACG808_003095"
     CDS             complement(4957..>5206)
                     /locus_tag="ACG808_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5048609.1"
                     /translation="EIDPRYLTNIENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLI
                     KSTRRLQVEKYMDSFTDKELSLMEALAKGINEARNIEG"
BASE COUNT         1805 a          835 c          926 g         1640 t
ORIGIN      
        1 tggaactcga acaagaggaa agcataagat ttcagaattg ttctctgttc cctctttatc
       61 atggaagtgc aaaaagtaat atagggattg ataaccttat agaagttatt actaataaat
      121 tttattcatc aacacatcga ggtccgtctg aactttgcgg aaatgttttc aaaattgaat
      181 atacaaaaaa aagacaacgt cttgcatatt aagtagttca gttttggagt acaaaaagtg
      241 aaaatgtaaa cctaaatgag tatcaaattg caactgaatt tgatactcgt tttttattgt
      301 taaatcaacg tttgttgtta atttacacca caaaatgtaa gttaaaatgt atataaaaat
      361 gcgaacgtga aataattatt gtaggaatgc aaattggtgt gttaaaatgg ttacaattta
      421 ctaagcaaaa aggggtgcat tttcctatgc aatttcttga gccaaaaaat aagaacgcta
      481 agtctgtgga ttgggaaata tcagagcaag taagggtgat agttaagcaa tacgctgaat
      541 atgcagagcg tactgaaagc gaagcagtgg atgaattctt attgaacatt ttagacgata
      601 agaagtttat tgaatggatt gccaacaagc gaagtaataa gcggattgtt gaaaaaatgg
      661 gtatcaaaga tagagtgggt tgatagacaa tgaccaagct taaaagatta gccacgaagg
      721 aagatgaaat tgtagatgtt aaaattccga tttcaaacga agaattaaaa gatagggcta
      781 aacaatatga tttattaact ccaaaacgat ttgcaacaag gtataacaaa atgttgttct
      841 tgccaacgtc ttttaaatgg aacggttctg aatatcccat tcaatataat tactgcatca
      901 atccgttttg ttgtaatttt gggaaagaac agcacaaatt caaagatgta aaagggaaac
      961 caagtcgata taaaatgact ggtagttcca aagataaagg tcataaaggt atgtactgca
     1021 atgacaatcc tattggcaga ggtgtatcgc aaaactgtac agtaacccct ttatctaatt
     1081 ggtctgttgt tgaagaaatt aaacgcctta tagaaatcaa tagcatccaa gatgttgagc
     1141 cagattatca atttcataaa gaaggctgct cggaagaaga atcaacccca ttcaatgagc
     1201 cgaagcaatt ttacaaaagg gggaaaagta ggggcaaatc tcaacgctat caatgtaagg
     1261 cgtgtaagaa gttcactaat gttctaccaa aaagagaaga aaccactact tatcaccaac
     1321 aaaagaacac cattcttcca atgttcgcta agatggttgt tggaagggtt tcagtcagtc
     1381 gtacttgtga tattttagga attggagttg gaacctacta tcataagctg gagtggctgt
     1441 ataggcgttg tttggagttt ttagaacgat atgagacaca accccttcaa acaaagaaat
     1501 ttaacgagat gtggcttaat acggacaaga tgcactacta cctaaataac gtccgtaaga
     1561 aaggacaagg ttcaaagaaa tatacggggt ttgaagacct taatatgcaa acgtatatag
     1621 ttgtatctgc tgaggtctta tcaagatatg tgttccgttc tgatgttgcc tatgattgga
     1681 atatttcaat ggatgaatta aatgaagata caagaaaatt caaagaagac catctcaata
     1741 cttttagtcg taaaaatgat aggttagatt ggtcttatta tccacaagaa ccatccgcta
     1801 acgatagcga aaataggaat gcttatcttc acgaattagg gaaaattact aataggagta
     1861 gatttgtcga tggtttaaat gttgatgctc cttatactac aactgctcac tattggttaa
     1921 ttaaacaaat ggttaatgcc gacgaatggc gaatgattag tgatgatgac ttctctatta
     1981 gaaacgcctt ttatagagta ttcacgaaag aattaaggtt gagcgatgca catcatttta
     2041 tttgccaagt taacaagacg aaaagcagaa aacaatgctt aaaagaattc ggacaagcaa
     2101 aagcagaact gctcgattgg ggagatatca gaggattcaa aacaaagttt ctacggactt
     2161 tggcatctca ttatctaact gaattactaa caagtcatca attccacgaa gaggctatta
     2221 gtaaagatgg agaaagatat cgcaaatatg ccgataaccc tatcaaacat ccattagcca
     2281 ctaaggataa aggattttat tcagttgatt gtagaacgga tttatctgct ctggaaccaa
     2341 acgaaatcgc aaagatgcta ttaaatgtta atgaccattc gaccaatagc tttattcaac
     2401 agataagacg ttatatatca tctcttgaaa gaccattaac aacagctcgt ggtgataaga
     2461 agagctatat ttacgctaac ttcaatccga agtatgctca atttgcgatt acaattctaa
     2521 gaacttacta caacttttgt cgccctttta aatcagcgga taaaaaggtt ttgacacctg
     2581 ctcaaagatt agggattaca gataaacaat ttgattggaa agatataatt tatttcaaat
     2641 gacaaaaaga gcttggagta atcccaagct cttgtgcttt atctttactg ttcttcttca
     2701 attaacgcac ccgttaactc aataagtttt ttctttcata tacatgaaaa tagtagttat
     2761 acggattttt atcattcttt atcccttttt gggcaaatac ctcattccat tcctcataat
     2821 tcacttctgg aaaaaaagta tctccttcga attcatgatg tatttttgtg atgtacattt
     2881 tctcaacata agggaaaaac aaattataaa tctgttctcc tccgaaaata aaaatttctt
     2941 cttcgttttt acataactca aaaacatctt ctattgaatg aacaatttca caaccattaa
     3001 aggtaaaccc cttatctctc gtcagaataa tatttcttct gtcaggtaag gctcttccga
     3061 ttgattcaag gttcttccta cctaatatta tcggatgtcc ctttgtagta tttttaacat
     3121 attcccagtc cttgggaatc ctccaaggaa tgtcattctc tttgccaatc actctattct
     3181 tatccatcgc agcaatcaaa gaaactttca tttataacac agccttttta actaatttac
     3241 catctaattt cttatttcta tcaaaaaacc ttttctcatt atcctgcccg ttagttgaat
     3301 aagcaatttt ttctataagt tatattatca taataccaca aaatcacaaa ttttcattca
     3361 attatcattt tcaattgcat tttataaatc attttgtatg taatttggta tgtaagttaa
     3421 aatgaaaaaa gtagtccatt ttgaagtaca ttttgtcatt ttgtactcca aaactgaact
     3481 acttacttgc atatatacgc ctttatagtg gagtactaca tttacgagat tcggttagag
     3541 tatcagaaaa agaaaaaata aaagttacag aaatgtatac ttcaataaat ggtgaattat
     3601 gtaagattga tagagcttat tctggagaaa ttgttatttt gcaaaatgag tttttgaagt
     3661 taaatagtgt tcttggagat acaaaactat tgccacagag aaaaaagatt gaaaatccgc
     3721 accctctact acaaacaact gttgaaccga gtaaacctga acagagagaa atgttgcttg
     3781 atgccctttt ggaaatctca gatagtgatc cgcttctacg atattacgtg gattctacga
     3841 cacatgaaat tatactttct ttcttaggga aagtacaaat ggaagtgatt agtgcactgt
     3901 tgcaagaaaa gtatcatgtg gagatagaac taaaagagcc tacagtcatt tatatggaga
     3961 gaccgttaaa aaatgcagaa tataccattc acatcgaagt gccgccaaat cctttctggg
     4021 cttccattgg tttatctgta tcaccgcttc cgttgggaag tggaatgcag tatgagagct
     4081 cggtttctct tggatactta aatcaatcat ttcaaaatgc agttatggaa gggatacgct
     4141 atggttgcga acaaggatta tatggttgga atgtgacgga ttgtaaaatc tgttttaagt
     4201 acggtttata ctatagccct gttagtactc cagcagattt tcggatgctt actcctattg
     4261 tactggagca agcctttaga aaagctggaa cagaattgtt agagccatat cttagtttta
     4321 aagtttatgc accacaggaa tatctttcac gggcatataa cgatgctccc aaatattgtg
     4381 caaatatcgt aaatactcaa ctgaaaaata atgaggtcat tattattgga gaaattcctg
     4441 ctcgatgtat tcaagattat cgcaatgatt taactttttt tacaaatggg cttagtgttt
     4501 gtttagcaga gctaaaagga tatcaggtta ccactggcga acctgtttgc cagacccgtc
     4561 gtctaaatag tcggatagat aaagtaagat atatgttcaa taaaataact tagtgcgttt
     4621 tatgttgtta tataaatatg gtttcttatt aaataagatg aaatattctt taatatagat
     4681 ttgaattaaa gtggaaagga ggagattgtt attataaact acaagtggat attgtgtcct
     4741 atttgtggaa ataaaacaag actacgaata cgagtggata ctatacttaa aaatttccct
     4801 ttatactgcc ccaaatgtaa gaacgaaact ttaattaatg ttcaaaaaat gaatataata
     4861 acaatcaaag agccagacgc caagacgcag agccgataat ttgagaaatg aaactctcat
     4921 cttatcggct ctttttgttt atctgaattt tactgactag ccttcaatat ttcttgcttc
     4981 attgatacct tttgctagtg cttccattaa ggatagttct ttgtctgtaa agctatccat
     5041 gtatttttct acctgtaacc ttcgagtgct tttaatcaaa ttatctgtag gtaagaaaaa
     5101 ttcatcaata gatacatgga gtaatgatac aagatcatac agcacttgtg tacttgggtg
     5161 ttgtcctttg ttttctatat ttgttaaata acgtgggtca atttca
//
LOCUS       DAZUMK010000332         3564 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.guided.188, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000332 DAZUMK010000000
VERSION     DAZUMK010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.guided.188"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="ACG808_003096"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="ACG808_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFS5048610.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2039..3412
                     /gene="tet(L)"
                     /locus_tag="ACG808_003097"
     CDS             2039..3412
                     /gene="tet(L)"
                     /locus_tag="ACG808_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HFS5048611.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
BASE COUNT         1128 a          541 c          728 g         1167 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg aattaattat
     2041 gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc tttgcgtttt
     2101 atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg atattgcaaa
     2161 tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta tgttaacctt
     2221 ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca aaaggttact
     2281 cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg gccattcttt
     2341 cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg catttccagc
     2401 actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta aagcatttgg
     2461 tcttattgga tcgatagtag ccatgggaga aggagtcggt ccagcgattg gtggaatgat
     2521 agcccattat attcattggt cctatcttct actcattcct atgataacaa ttatcactgt
     2581 tccgtttctt atgaaattat taaagaaaga agtaaggata aaaggtcatt ttgatatcaa
     2641 aggaattata ctaatgtctg taggcattgt attttttatg ttgtttacaa catcatatag
     2701 catttctttt cttatcgtta gcgtgctgtc attcctgata tttgtaaaac atatcaggaa
     2761 agtaacagat ccttttgttg atcccggatt agggaaaaat atacctttta tgattggagt
     2821 tctttgtggg ggaattatat ttggaacagt agcagggttt gtctctatgg ttccttatat
     2881 gatgaaagat gttcaccagc taagtactgc cgaaatcgga agtgtaatta ttttccctgg
     2941 aacaatgagt gtcattattt tcggctacat tggtgggata cttgttgata gaagaggtcc
     3001 tttatacgtg ttaaacatcg gagttacatt tctttctgtt agctttttaa ctgcttcctt
     3061 tcttttagaa acaacatcat ggttcatgac aattataatc gtatttgttt taggtgggct
     3121 ttcgttcacc aaaacagtta tatcaacaat tgtttcaagt agcttgaaac agcaggaagc
     3181 tggtgctgga atgagtttgc ttaactttac cagcttttta tcagagggaa caggtattgc
     3241 aattgtaggt ggtttattat ccataccctt acttgatcaa aggttgttac ctatggaagt
     3301 tgatcagtca acttatctgt atagtaattt gttattactt ttttcaggaa tcattgtcat
     3361 tagttggctg gttaccttga atgtatataa acattctcaa agggatttct aaatcgttaa
     3421 gggatcaact ttgggagaga gttcaaaatt gatccttttt ttataacagg aattcaaatc
     3481 tttttgttcc attaaagggc gcgattgctg aataaaagat acgagagacc tctcttgtat
     3541 cttttttatt ttgagtggtt ttgt
//
LOCUS       DAZUMK010000333         3564 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.guided.189, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000333 DAZUMK010000000
VERSION     DAZUMK010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.guided.189"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="ACG808_003098"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="ACG808_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFS5048612.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2039..3412
                     /gene="tet(L)"
                     /locus_tag="ACG808_003099"
     CDS             2039..3412
                     /gene="tet(L)"
                     /locus_tag="ACG808_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HFS5048613.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
BASE COUNT         1127 a          541 c          729 g         1167 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg aattaattat
     2041 gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc tttgcgtttt
     2101 atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg atattgcaaa
     2161 tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta tgttaacctt
     2221 ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca aaaggttact
     2281 cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg gccattcttt
     2341 cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg catttccagc
     2401 actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta aagcatttgg
     2461 tcttattgga tcgatagtag ccatgggaga aggagtcggt ccagcgattg gtggaatgat
     2521 agcccattat attcattggt cctatcttct actcattcct atgataacaa ttatcactgt
     2581 tccgtttctt atgaaattat taaagaaaga agtaaggata aaaggtcatt ttgatatcaa
     2641 aggaattata ctaatgtctg taggcattgt attttttatg ttgtttacaa catcatatag
     2701 catttctttt cttatcgtta gcgtgctgtc attcctgata tttgtaaaac atatcaggaa
     2761 agtaacagat ccttttgttg atcccggatt agggaaaaat atacctttta tgattggagt
     2821 tctttgtggg ggaattatat ttggaacagt agcagggttt gtctctatgg ttccttatat
     2881 gatgaaagat gttcaccagc taagtactgc cgaaatcgga agtgtaatta ttttccctgg
     2941 aacaatgagt gtcattattt tcggctacat tggtgggata cttgttgata gaagaggtcc
     3001 tttatacgtg ttaaacatcg gagttacatt tctttctgtt agctttttaa ctgcttcctt
     3061 tcttttagaa acaacatcat ggttcatgac aattataatc gtatttgttt taggtgggct
     3121 ttcgttcacc aaaacagtta tatcaacaat tgtttcaagt agcttgaaac agcaggaagc
     3181 tggtgctgga atgagtttgc ttaactttac cagcttttta tcagagggaa caggtattgc
     3241 aattgtaggt ggtttattat ccataccctt acttgatcaa aggttgttac ctatggaagt
     3301 tgatcagtca acttatctgt atagtaattt gttattactt ttttcaggaa tcattgtcat
     3361 tagttggctg gttaccttga atgtatataa acattctcaa agggatttct aaatcgttaa
     3421 gggatcaact ttgggagaga gttcaaaatt gatccttttt ttataacagg aattcaaatc
     3481 tttttgttcc attaaagggc gcgattgctg aataaaagat acgagagacc tctcttgtat
     3541 cttttttatt ttgagtggtt ttgt
//
LOCUS       DAZUMK010000334         2534 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.guided.208, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000334 DAZUMK010000000
VERSION     DAZUMK010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.guided.208"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="ACG808_003100"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="ACG808_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFS5048614.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            2039..2227
                     /locus_tag="ACG808_003101"
     CDS             2039..2227
                     /locus_tag="ACG808_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFS5048615.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(2285..>2534)
                     /locus_tag="ACG808_003102"
     CDS             complement(2285..>2534)
                     /locus_tag="ACG808_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5048616.1"
                     /translation="EIDPRYLTNIENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLI
                     KSTRRLQVEKYMDSFTDKELSLMEALAKGINEARNIEG"
BASE COUNT          863 a          393 c          500 g          778 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtac ggtttatact atagccctgt tagtactcca
     1561 gcagattttc ggatgcttac tcctattgta ctggagcaag cctttagaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa gtttatgcac cacaggaata tctttcacgg
     1681 gcatataacg atgctcccaa atattgtgca aatatcgtaa atactcaact gaaaaataat
     1741 gaggtcatta ttattggaga aattcctgct cgatgtattc aagattatcg caatgattta
     1801 acttttttta caaatgggct tagtgtttgt ttagcagagc taaaaggata tcaggttacc
     1861 actggcgaac ctgtttgcca gacccgtcgt ctaaatagtc ggatagataa agtaagatat
     1921 atgttcaata aaataactta gtgcgtttta tgttgttata taaatatggt ttcttattaa
     1981 ataagatgaa atattcttta atatagattt gaattaaagt ggaaaggagg agattgttat
     2041 tataaactac aagtggatat tgtgtcctat ttgtggaaat aaaacaagac tacgaatacg
     2101 agtggatact atacttaaaa atttcccttt atactgcccc aaatgtaaga acgaaacttt
     2161 aattaatgtt caaaaaatga atataataac aatcaaagag ccagacgcca agacgcagag
     2221 ccgataattt gagaaatgaa actctcatct tatcggctct ttttgtttat ctgaatttta
     2281 ctgactagcc ttcaatattt cttgcttcat tgataccttt tgctagtgct tccattaagg
     2341 atagttcttt gtctgtaaag ctatccatgt atttttctac ctgtaacctt cgagtgcttt
     2401 taatcaaatt atctgtaggt aagaaaaatt catcaataga tacatggagt aatgatacaa
     2461 gatcatacag cacttgtgta cttgggtgtt gtcctttgtt ttctatattt gttaaataac
     2521 gtgggtcaat ttca
//
LOCUS       DAZUMK010000335         2534 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.guided.209, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000335 DAZUMK010000000
VERSION     DAZUMK010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.guided.209"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="ACG808_003103"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="ACG808_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFS5048617.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            2039..2227
                     /locus_tag="ACG808_003104"
     CDS             2039..2227
                     /locus_tag="ACG808_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFS5048618.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(2285..>2534)
                     /locus_tag="ACG808_003105"
     CDS             complement(2285..>2534)
                     /locus_tag="ACG808_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5048619.1"
                     /translation="EIDPRYLTNIENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLI
                     KSTRRLQVEKYMDSFTDKELSLMEALAKGINEARNIEG"
BASE COUNT          862 a          393 c          501 g          778 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtac ggtttatact atagccctgt tagtactcca
     1561 gcagattttc ggatgcttac tcctattgta ctggagcaag cctttagaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa gtttatgcac cacaggaata tctttcacgg
     1681 gcatataacg atgctcccaa atattgtgca aatatcgtaa atactcaact gaaaaataat
     1741 gaggtcatta ttattggaga aattcctgct cgatgtattc aagattatcg caatgattta
     1801 acttttttta caaatgggct tagtgtttgt ttagcagagc taaaaggata tcaggttacc
     1861 actggcgaac ctgtttgcca gacccgtcgt ctaaatagtc ggatagataa agtaagatat
     1921 atgttcaata aaataactta gtgcgtttta tgttgttata taaatatggt ttcttattaa
     1981 ataagatgaa atattcttta atatagattt gaattaaagt ggaaaggagg agattgttat
     2041 tataaactac aagtggatat tgtgtcctat ttgtggaaat aaaacaagac tacgaatacg
     2101 agtggatact atacttaaaa atttcccttt atactgcccc aaatgtaaga acgaaacttt
     2161 aattaatgtt caaaaaatga atataataac aatcaaagag ccagacgcca agacgcagag
     2221 ccgataattt gagaaatgaa actctcatct tatcggctct ttttgtttat ctgaatttta
     2281 ctgactagcc ttcaatattt cttgcttcat tgataccttt tgctagtgct tccattaagg
     2341 atagttcttt gtctgtaaag ctatccatgt atttttctac ctgtaacctt cgagtgcttt
     2401 taatcaaatt atctgtaggt aagaaaaatt catcaataga tacatggagt aatgatacaa
     2461 gatcatacag cacttgtgta cttgggtgtt gtcctttgtt ttctatattt gttaaataac
     2521 gtgggtcaat ttca
//
LOCUS       DAZUMK010000336         2003 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.guided.230, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000336 DAZUMK010000000
VERSION     DAZUMK010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.guided.230"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            70..419
                     /locus_tag="ACG808_003106"
                     /pseudo
     CDS             70..419
                     /locus_tag="ACG808_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(691..981)
                     /locus_tag="ACG808_003107"
     CDS             complement(691..981)
                     /locus_tag="ACG808_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5048620.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1189..1880)
                     /locus_tag="ACG808_003108"
                     /pseudo
     CDS             complement(1189..1880)
                     /locus_tag="ACG808_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          560 a          378 c          300 g          765 t
ORIGIN      
        1 ctaatagagt ataacatagc tttaagcatt acttaaagat aacttaaaag ttatctttta
       61 aatacttaat tgttaaatac cttatttgat gcaaaagaaa tctacgatag tcaacaaaaa
      121 aattttcatt atatccagaa aaatttttct tagttaagga cctatggatt gcagtaatgc
      181 agaattcgtc gaataaactg ccaaagacat acaatcatat tgcttttaaa attgacgaac
      241 aagatataga ttcgtttgtc tcaaaaatcc aaatgttagg tttaactgtc gaaccaggac
      301 gttctcgtgt gaaaggcgag gcttcttcaa tttactttta cgactatgat aatcacctct
      361 tcgagttaca taccggtact ctccaggaac gtttaaaatc ctacaattcc actacctgag
      421 actagtaatg actagtctct ttttatggcc tctgatcccc ccattgagcg aatactaata
      481 gggcttcgaa ttgatctgtt caatctttaa gcaatatccc tcaaatgagt ttggaaagtt
      541 tagtgattca tcacaaatac ttaaatacat gtagttatat atagattgag gttcttgtga
      601 gcgtatgctc gtgtgggttc aaatcccatc gtctgcatcc atctttccaa aacaagcggc
      661 taggacattt ttgtctcagc cgcttgtttt ctagtttatt ctttcatctt gtcggacctt
      721 ttttcttggg tatagacttt tctcatattc taccacaatt ttgggtttcc ctaacacatg
      781 ataggatctc acaacaaatt gctttctatt attataataa cctgtaacga ctaacttcat
      841 cccttcgtct acttcagcta ggaagttcaa actatgggaa gcaatcaaac aattgacccc
      901 atttaaactg aaacggatca acggtctggc actaacttgc aaagtacgga tcttatctac
      961 tgttccatat tttttctcca taaccttcac ctcaatacca ttatacgaac atacattccc
     1021 tttgtaaaga agaaactctg ttttatcttt ttgacttttt gtctattacc tttcttaaaa
     1081 tcatattggc acttcctgcg aagatgaagg acatgtacta aaaaaaagag gcagcagtat
     1141 agattactgt tgcttcttta ctaatttagg tttacttgtt aagaaagatt atttacgcaa
     1201 atgttcttca acaatttgct gagatttatc agctgtatct tccacttcat cttttgcttt
     1261 ttctaacttt tgaatatatt gtttcaagct tgagttttca gcagttgctt cgtctaattt
     1321 cttttgtgta tctgttaact gatttctaca tttgtttttt cttgtgtcag tgttgcgatt
     1381 tgttgattca aaccatttac agtattttgt aattctgaaa gttttgcttg tgcttcttgt
     1441 agtcttgctg tccaatctgc attcagatga ttgatttcat catttttttg gttgatctca
     1501 tctagtttga cattgatttc attttgtttg ctttgtagtt gtgcatcctt gctgactaat
     1561 tgactttcta agttttgttt ttctgctgcc gattgatttt tttggttttc taattcattt
     1621 acttttgttt ggtattcttg gacatctgcc tttgaatctt gcaatgtctg ttctttttgt
     1681 gctagtcgag cggtaagatc tgtaatcgct tgctctttat ctgtcaatgt atgatcaagc
     1741 tgtgtcagat tctcattgat tgtatcaata tctgtctctc caccccacaa aataatttca
     1801 tttgcgtacc gcgtgcctgt gtagccaatt agacatagaa atgcaacgac aatcccacca
     1861 gtaataaatt tctttttcat ttgcatagct ccttaatcca atacttttac atgctaaaag
     1921 taagtgatct caaacacaat gtcaagcgtt tttaaattac atatgtaatt ttaatataaa
     1981 tactatcaaa tactcattta atc
//
LOCUS       DAZUMK010000337         1843 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.guided.237, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000337 DAZUMK010000000
VERSION     DAZUMK010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.guided.237"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..636
                     /locus_tag="ACG808_003109"
     CDS             <1..636
                     /locus_tag="ACG808_003109"
                     /inference="COORDINATES: protein motif:HMM:NF045597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3072 family TudS-related putative desulfidase"
                     /protein_id="HFS5048621.1"
                     /translation="YSSTVEIGKSDWNNHTLIFGLIIKICEFVRVYTMFNDGRSKKVV
                     FVAHCFLNQNSISDGTAIYPAANKDVVDFFLNADIGIVQMPCPEFCCLGLDRGNIHGA
                     DSPVVVENTRIRSAMQNDESNLKLTRLADYVVQHIMEYKKYGFEVIGIIGANRSPNCG
                     VETTSDNDAEINGMGLFIDKIFRRLLRENISIPMIGIKGTDNIQEKLQQLI"
     gene            722..1213
                     /locus_tag="ACG808_003110"
     CDS             722..1213
                     /locus_tag="ACG808_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside adenylyltransferase"
                     /protein_id="HFS5048622.1"
                     /translation="MDKKETATKEDLMAVVSLFENMGILYWVDGGWGVDLLAGKQTRD
                     HRDIDINFDAQYTERLLDILSEHGYKVETDWKPVRIELYSDEYGYLDIHPFVLNDDGT
                     SKQADLEGGWYEFEKDYFGSAVFEGRTIPCISLKGQKIFHSGYELRDKDKHDLSVLES
                     ISE"
     gene            1232..1780
                     /locus_tag="ACG808_003111"
     CDS             1232..1780
                     /locus_tag="ACG808_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HFS5048623.1"
                     /translation="MEEATRYIALLRGVNISGKNKVPMAELKKIFEELGFGAVKTYLN
                     SGNVIFSSDEENIGSLTDRIETTIKKWFGLDIPIFVISKEDLEDILQHAPAWWGDENK
                     EIYDNLIFIMPPATFAEVWGEIGEPREGLEKIKNYKAAVFWSFSRKDYQKTNWWRETA
                     SANISGKLTIRTANTVRKIVGM"
BASE COUNT          613 a          252 c          460 g          518 t
ORIGIN      
        1 tacagttcca cggtggaaat tggtaaaagt gattggaaca atcatacact gatttttggg
       61 ttaataatta aaatttgtga atttgtgaga gtatatacta tgttcaatga tggaagaagt
      121 aaaaaggttg tttttgttgc acattgcttt ttaaatcaaa attccatttc agatggaacg
      181 gcgatttatc cggcagcaaa taaagatgtt gttgattttt tcttaaatgc agatattggt
      241 attgtccaaa tgccctgtcc ggagttttgc tgtttaggac ttgatagggg aaatattcat
      301 ggagctgata gcccggtagt ggtagagaat acacgcatac gttcagcaat gcaaaatgac
      361 gaatcgaatt tgaaactaac aagactggct gattatgtag tacaacatat catggaatat
      421 aaaaaatatg gatttgaagt tatcggtatt attggggcaa atcgttctcc aaattgtggt
      481 gtcgaaacaa cttcggacaa tgatgcagaa atcaatggta tgggattgtt tatagataaa
      541 atatttcgcc ggcttttgcg agaaaacata tctattccca tgataggcat aaagggaacg
      601 gacaatatac aagaaaaact tcagcagtta atatgagtga gagccgaacc aaacagcggt
      661 tgctaccgtg aatatcaaaa aatatgattg atagtttcag aattggaaaa ggaggagttg
      721 catggataaa aaagaaacgg caacaaaaga agacttgatg gcagtcgtta gcctgtttga
      781 aaatatggga atactatatt gggtggacgg tggctggggc gttgatctat tggcgggtaa
      841 acagacaagg gatcatagag atattgatat caactttgac gctcaatata cagaaaggct
      901 attagatata ctttcggagc atggttataa agtagagacg gattggaaac ctgttagaat
      961 agagttatat agcgatgaat acggttattt ggacatccat ccatttgttt tgaatgatga
     1021 cgggacgtca aaacaagccg atttggaagg cggctggtac gagtttgaga aggactattt
     1081 tggtagtgct gtttttgagg gtagaacaat tccctgtata tctttgaaag gacaaaaaat
     1141 ttttcactcg ggttatgagc taagggataa ggacaagcat gatctttctg tccttgaaag
     1201 tatatcagaa taaggcgaaa tggtgatttg catggaggaa gcaacgagat atattgcgct
     1261 tttgcgcggt gttaatataa gtggaaaaaa taaagttccg atggctgaac tgaagaaaat
     1321 ttttgaggag cttgggttcg gagcggttaa gacctatctg aacagtggca atgtgatttt
     1381 ttcaagtgat gaagagaata tagggagcct tacagaccgg attgaaacaa cgataaaaaa
     1441 gtggtttggt ctggatattc cgattttcgt tatatcaaaa gaggatctcg aagatatttt
     1501 gcaacatgcg ccggcttggt ggggcgatga aaataaagaa atctatgata atctgatttt
     1561 tattatgcct ccggctacat ttgctgaagt atggggcgag atcggagaac ccagggaagg
     1621 attagaaaag attaagaatt ataaggcagc ggtattctgg tctttcagcc gaaaggatta
     1681 tcaaaaaaca aactggtgga gggagacggc aagtgcgaac atcagcggca aactgacaat
     1741 aagaacggca aacactgtca ggaagattgt cggaatgtag ttgtttctca aattccggtt
     1801 tgtggggtga aaaatatgca gaacaacgaa ttactgaatc att
//
LOCUS       DAZUMK010000338         1211 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.guided.268, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000338 DAZUMK010000000
VERSION     DAZUMK010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.guided.268"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1092
                     /locus_tag="ACG808_003112"
                     /note="aph(2'')-Ia"
     CDS             <1..1092
                     /locus_tag="ACG808_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HFS5048624.1"
                     /translation="IKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          522 a           92 c          200 g          397 t
ORIGIN      
        1 attaaattga tttttgaatt tttgaaaaaa gaaagaaatg ctaatgcagt tattttagac
       61 cctcataaaa ataatccaag agcaataagg gcataccaaa aatctggttt tagaattatt
      121 gaagatttgc cagaacatga attacacgag ggcaaaaaag aagattgtta tttaatggaa
      181 tatagatatg atgataatgc cacaaatgtt aaggcaatga aatatttaat tgagcattac
      241 tttgataatt tcaaagtaga tagtattgaa ataatcggta gtggttatga tagtgtggca
      301 tatttagtta ataatgaata catttttaaa acaaaattta gtactaataa gaaaaaaggt
      361 tatgcaaaag aaaaagcaat atataatttt ttaaatacaa atttagaaac taatgtaaaa
      421 attcctaata ttgaatattc gtatattagt gatgaattat ctatactagg ttataaagaa
      481 attaaaggaa cttttttaac accagaaatt tattctacta tgtcagaaga agaacaaaat
      541 ttgttaaaac gagatattgc cagtttttta agacaaatgc acggtttaga ttatacagat
      601 attagtgaat gtactattga taataaacaa aatgtattag aagagtatat attgttgcgt
      661 gaaactattt ataatgattt aactgatata gaaaaagatt atatagaaag ttttatggaa
      721 agactaaatg caacaacagt ttttgagggt aaaaagtgtt tatgccataa tgattttagt
      781 tgtaatcatc tattgttaga tggcaataat agattaactg gaataattga ttttggagat
      841 tctggaatta tagatgaata ttgtgatttt atatacttac ttgaagatag tgaagaagaa
      901 ataggaacaa attttggaga agatatatta agaatgtatg gaaatataga tattgagaaa
      961 gcaaaagaat atcaagatat agttgaagaa tattatccta ttgaaactat tgtttatgga
     1021 attaaaaata ttaaacagga atttatcgaa aatggtagaa aagaaattta taaaaggact
     1081 tataaagatt gattatataa tatatgaaaa gctattataa aagacattag tattaaatag
     1141 tttaaaaaaa tgaaaaataa taaaggaagt gagtcaagtc cagactcctg tgtaaaatgc
     1201 tatacaatgt t
//
LOCUS       DAZUMK010000339          942 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.guided.281, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000339 DAZUMK010000000
VERSION     DAZUMK010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.guided.281"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            329..877
                     /gene="aac(6')"
                     /locus_tag="ACG808_003113"
     CDS             329..877
                     /gene="aac(6')"
                     /locus_tag="ACG808_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFS5048625.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          336 a          135 c          189 g          282 t
ORIGIN      
        1 ccctttgctt ttttgtaatt atctaaaaat tctaaaaatt aagattatta tatgaaaaca
       61 ggttttattt gtcaattcat aattggtttt ttttaaagca aaagataatc tatatcatga
      121 tgaacaaaca gagttttttc ctccaattga tggctttcct gatctttcct attttgaata
      181 aagtgtttct gtataatttt tttattttat aagtaagata aataatgaaa gaatagagat
      241 actgattggt agctaaagat ttttgagcga gaacatttcg ttttttcaaa aggactgtta
      301 gaataagcaa gaacaagggg gaagacgtat gataatcagt gaatttgacc gtaataatcc
      361 agtattgaaa gatcagcttt ctgatttact gagactgact tggccggaag aatatggaga
      421 cagctcggca gaagaagtag aagaaatgat gaatccagaa cgaatcgcgg tagcagcggt
      481 agaccaagat gagttagtag gatttattgg tgcaatccct caatacggta tcacaggttg
      541 ggaattgcat ccattagttg tagaaagctc ccgacgaaag aaccaaatag gtactcgatt
      601 agtcaattac ttagaaaaag aagtagcttc cagaggagga atcacgattt atttaggtac
      661 ggatgattta gaccatggaa caacgttaag tcaaacggac ctgtatgagc atacatttga
      721 taaagtggct tccatccaga accttcgtga acatccgtat gaattctatg aaaaattagg
      781 ttataaaatc gtaggtgtct taccaaatgc aaatggctgg gacaaaccgg atatttggat
      841 ggcaaaaacg attattcctc gaccaaattc tcaataataa aaagaggttg tgacagaagt
      901 aatcagcttc aagaaataag aaggaattta cgaaaattac tt
//
LOCUS       DAZUMK010000340          737 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5071
            SAMEA115707476-rid22580343.guided.294, whole genome shotgun
            sequence.
ACCESSION   DAZUMK010000340 DAZUMK010000000
VERSION     DAZUMK010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707476
            Sequence Read Archive: ERR13197205
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288035.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288035.1
            Genome Coverage       :: 137x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,051
            Genes (coding)                    :: 2,959
            CDSs (with protein)               :: 2,959
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 5 (16S, 23S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 38 of 92
            Pseudo Genes (incomplete)         :: 48 of 92
            Pseudo Genes (internal stop)      :: 26 of 92
            Pseudo Genes (multiple problems)  :: 15 of 92
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707476-rid22580343.guided.294"
                     /isolate="VRE5071"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>737
                     /gene="aac(6')-Ie"
                     /locus_tag="ACG808_003114"
     CDS             <1..>737
                     /gene="aac(6')-Ie"
                     /locus_tag="ACG808_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ie"
                     /protein_id="HFS5048626.1"
                     /translation="NEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLESLKKH
                     YTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFIGEPNY
                     WSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLPEHELH
                     EGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEY
                     IFKTKFSTNKKKGYAKEKAIYNFLNTN"
BASE COUNT          313 a           58 c          127 g          239 t
ORIGIN      
        1 aaaatgaaat atgtataaga actttaatag atgatgattt tcctttgatg ttaaaatggt
       61 taactgatga aagagtatta gaattttatg atggtagaga taaaaaatat acattagaat
      121 cattaaaaaa acattataca gagccttggg aagatgaagt ttttagagta attattgaat
      181 ataacaatgt tcctattgga tatggacaaa tatataaaat gtatgatgag ttatatactg
      241 attatcatta tccaaaaact gatgagatag tctatggtat ggatcaattt ataggagagc
      301 caaattattg gagtaaagga attggtacaa gatatattaa attgattttt gaatttttga
      361 aaaaagaaag aaatgctaat gcagttattt tagaccctca taaaaataat ccaagagcaa
      421 taagggcata ccaaaaatct ggttttagaa ttattgaaga tttgccagaa catgaattac
      481 acgagggcaa aaaagaagat tgttatttaa tggaatatag atatgatgat aatgccacaa
      541 atgttaaggc aatgaaatat ttaattgagc attactttga taatttcaaa gtagatagta
      601 ttgaaataat cggtagtggt tatgatagtg tggcatattt agttaataat gaatacattt
      661 ttaaaacaaa atttagtact aataagaaaa aaggttatgc aaaagaaaaa gcaatatata
      721 attttttaaa tacaaat
//
